LOCUS       DAHHRR010000001        71197 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.001, whole genome shotgun sequence.
ACCESSION   DAHHRR010000001 DAHHRR010000000
VERSION     DAHHRR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 71197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71197
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.001"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(161..1096)
                     /locus_tag="NBI42_000001"
     CDS             complement(161..1096)
                     /locus_tag="NBI42_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCD1776468.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIVEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEQQSPLTYGTMAV
                     DLNHVTGKPANAYFATTVDVEEVWNLIDHKLRTYE"
     gene            1459..2988
                     /locus_tag="NBI42_000002"
     CDS             1459..2988
                     /locus_tag="NBI42_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1776469.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKVAAQQVEATERAQTIVAGLGGEDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEVEELLG
                     D"
     gene            complement(3309..4262)
                     /locus_tag="NBI42_000003"
     CDS             complement(3309..4262)
                     /locus_tag="NBI42_000003"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCD1776470.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(4573..4680)
                     /locus_tag="NBI42_000004"
     CDS             complement(4573..4680)
                     /locus_tag="NBI42_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776471.1"
                     /translation="MSVCTSTIARIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            4835..5980
                     /locus_tag="NBI42_000005"
     CDS             4835..5980
                     /locus_tag="NBI42_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HCD1776472.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSTFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLGVIKPVIMPVAYKHCALQVYAEHYPIVGENL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKDIYDQMMWIGFKPFKITNIKQE
                     SADIKSFTVETDAYDFSQFTPGQYITVDVSSDKLPYRAKRHYSIVSGDKNHLTFGVKR
                     DVTIDHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMF
                     EAASAKGIETQMVQVAENEEHLPFKDNFDSIARRHGNANVYTHLKDEQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            6006..6179
                     /locus_tag="NBI42_000006"
     CDS             6006..6179
                     /locus_tag="NBI42_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776473.1"
                     /translation="MKLNQRYVKVFALYFVSIITANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            6291..6647
                     /locus_tag="NBI42_000007"
     CDS             6291..6647
                     /locus_tag="NBI42_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCD1776474.1"
                     /translation="MTIDIGRIYNHKERTDAIRVLVDRVWPRGMSKSTANLDYWLKDV
                     APSTELRKWFHHNPKLFGAFKEKYKKELRDNDAQNAAFKELKALVEKHNHVLLLYAAK
                     DIEHNQAVILQQLLNT"
     gene            6845..8323
                     /locus_tag="NBI42_000008"
     CDS             6845..8323
                     /locus_tag="NBI42_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1776475.1"
                     /translation="MKFKSLATIIAAATLVLSGCGSTHSSGKDLNISLPLKTKSIAPY
                     ETDVPVKIGAAESLFKTNDQGKIEKVLVKSYHQPNDTTLDLELKDNIKFQNGQKLTAE
                     KVKSSLENSMKKSDLVKFSLPISSITAKGQKLTIKTKSAYPELVSELANPFMAIYDTD
                     AKSDVNQTPVGTGPYEIKDYKQSQKISLAKFKDYWQGKPKLDHITVTYQEDGNNRVRN
                     LESKKADLITDVPVNKVEDITSDKHLKVSKKSGFRTSLLMYNHTNKKMTKPVREALDN
                     VIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIDKAKDLLAKEGYTKNHPL
                     KIKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLTDRSAWDATMYSFGT
                     IPRGDTGYYFNQAYKKDGAINKGNYDNSKVDDLIDQLNHTVNVDERHNISNDIIKLSS
                     QDVPNSYIAYNDQIVAANSNVKNYKVTPEGIYLIDYQTTIER"
     gene            complement(8725..9627)
                     /locus_tag="NBI42_000009"
     CDS             complement(8725..9627)
                     /locus_tag="NBI42_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1776476.1"
                     /translation="MEKVNFKSNNLNVVANMYFPENFDENNQYPAIVVNHPAGGVKEQ
                     TAGLYAERLATLGYVTMAYDASYQGESEGQPHNLENPSSRVEDVRAAVDYFNTLDFID
                     DSRIGALGICAGGGYTIKAAQTEKRIKAVVGISAADIGQNFRKGWTGNQDEKDINPLL
                     EQVAEQRKAEANGAPQKLVGFVPEEPTEDMDQETKDGWEYYRTPRAQHERSINQFPFI
                     SFDRIIEFTAFDLVDKLLTQPVLFIAGSEAGTLWQSENAYERALEPKDIHIVEGANHF
                     DMYDKEPFVTEAVEKMKSFYGQYL"
     gene            complement(9640..10284)
                     /locus_tag="NBI42_000010"
     CDS             complement(9640..10284)
                     /locus_tag="NBI42_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1776477.1"
                     /translation="MEKKNILIIGASGQISQELIDLLLDKTEHHLTLYLRNKAKLMNI
                     EDGKRVTLIEADATDEAALENALDQIDVVFASLAGNIDEQAHAIVSAMEKKNVQRLIF
                     VTALGIYDEVPGAFGKWNNDNIGPYLPPYKKAAEIIEASSLDYTVLRPAWLTNNDEIE
                     YETTIKGEDFKGTEVSRKSVAALGLEIIENPSIHVNESLGVNKPNTDGDKPHWL"
     gene            10438..10989
                     /locus_tag="NBI42_000011"
     CDS             10438..10989
                     /locus_tag="NBI42_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1776478.1"
                     /translation="MDKRIKKTQRTLKQTMITLLKEQHIEDITVTDICDKSEIVRRTF
                     YAHFNDKYDLIEHIMSDYISDFVEICHFKEQINFKLGNIKWFEYLYNNKDIFSVLFKS
                     SISALFSKKLETVIYKQLENKISESVINEGISKSTLLCFLTSAILGVMVNFALNGDSD
                     YQNKADETLLLLKPYLKDDILNS"
     gene            complement(11118..12260)
                     /locus_tag="NBI42_000012"
     CDS             complement(11118..12260)
                     /locus_tag="NBI42_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCD1776479.1"
                     /translation="MSSAITQSTKTSDIHNESIDKQMEAKAHETAQNADLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDILDKRFLIFFLSAVGLHGIWDTSLTI
                     LGSDTLKIFILIVVVWILVFILMGAGLKQVNTLQKEFKEQRKKVDE"
     gene            12701..14278
                     /locus_tag="NBI42_000013"
     CDS             12701..14278
                     /locus_tag="NBI42_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCD1776480.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLLYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNTKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALPGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVKKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEVYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            14304..15809
                     /locus_tag="NBI42_000014"
     CDS             14304..15809
                     /locus_tag="NBI42_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCD1776481.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLIDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILDQHMRSHLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            15921..17132
                     /locus_tag="NBI42_000015"
     CDS             15921..17132
                     /locus_tag="NBI42_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCD1776482.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDCPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            17318..19579
                     /locus_tag="NBI42_000016"
     CDS             17318..19579
                     /locus_tag="NBI42_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HCD1776483.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIIVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKLSIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFENGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDVIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDSGFYKQDETIEYATPVSHFVKDELWDKGDAKLSITHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENEHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPIVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILCTSHKFDDKQASMTKVLTNIAFARVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPKPKYKYIGLGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            19609..20793
                     /locus_tag="NBI42_000017"
     CDS             19609..20793
                     /locus_tag="NBI42_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCD1776484.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRTGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKNEIIPIQVNSVEYTNAGPKIHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GYKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            21091..21198
                     /locus_tag="NBI42_000018"
     CDS             21091..21198
                     /locus_tag="NBI42_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776485.1"
                     /translation="MCVRTRLISSSSVRLSKAIIIAVIVVYHIDVRGLF"
     gene            complement(21380..23356)
                     /gene="coa"
                     /locus_tag="NBI42_000019"
     CDS             complement(21380..23356)
                     /gene="coa"
                     /locus_tag="NBI42_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HCD1776486.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSKESRVNENSKK
                     GATVSDYYYWKIIDSLEAQFTGAIDLLEDYKYGDSIYKEAKDRLMTRVLGEDQYLLKK
                     KIDEYELYKKWYKSSNKNTNMLTFHKYNLYNLTMNEYNDIFNSLKDAVYQFNKEVKEI
                     EHKNVDLKQFDKDGEDKATKEVYDLVSEIDTLVVTYYADKDYGEHAKELRAKLDLILG
                     DTDNPHKVTNERIKKEMIDDLNSIIDDFFMETKQNRPNSITKYDPTKHNFKEKSENKP
                     NFDKLVEETKKAVKEADESWKNKTVKKYEETVTKSPIVKEEKKVKEPQLPKVGNQQEV
                     KTTAGKADETTQPVAQPLVKIPQGTIYGETVKGPDYPTMENKTLQGEIVQGPDFPTME
                     QNRPSLSNNYTNPTLTNPILEGLEGSSSKLEIKPQGTESTLKGIQGESSDIEVKPQAS
                     ETTEASHYPARPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYNVT
                     THGNGQVSYGARPTQNKPSKTNAYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGN
                     GQVSYGARPTQNKPSKTNAYNVTTHANGQVSYGARPTQNKPSKTNEYNVTTHANGQVS
                     YGARPTYKKPSETNAYNVTTHADGTATYGPRVTK"
     gene            23546..23890
                     /locus_tag="NBI42_000020"
     CDS             23546..23890
                     /locus_tag="NBI42_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCD1776487.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQRQALV
                     DQLHELIANTDLNKLSCLNLDAYQKRDILAAHYIAKSAIRTKNLDQMTKAKHRLESIY
                     DSISNPLHSQNN"
     gene            complement(24054..25817)
                     /locus_tag="NBI42_000021"
     CDS             complement(24054..25817)
                     /locus_tag="NBI42_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HCD1776488.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWMEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQVITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMVAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATFLAIYVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSKFFIVSMLLAVTCFIGYNMYLLYN
                     STINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDVVGLKMRQNGREAKLVSLDEFMETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(26138..26893)
                     /gene="pflA"
                     /locus_tag="NBI42_000022"
     CDS             complement(26138..26893)
                     /gene="pflA"
                     /locus_tag="NBI42_000022"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCD1776489.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            complement(26916..29165)
                     /gene="pflB"
                     /locus_tag="NBI42_000023"
     CDS             complement(26916..29165)
                     /gene="pflB"
                     /locus_tag="NBI42_000023"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCD1776490.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            29753..30721
                     /locus_tag="NBI42_000024"
     CDS             29753..30721
                     /locus_tag="NBI42_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD1776491.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQDHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIKGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            30718..32274
                     /locus_tag="NBI42_000025"
     CDS             30718..32274
                     /locus_tag="NBI42_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1776492.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     SRQFALIVDQYDNILYANDDRFTVGEKYRPQQFGFMNESVKLNHANHRLIIYKDIHEN
                     IEDGITLLIVMAIVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            32267..33025
                     /locus_tag="NBI42_000026"
     CDS             32267..33025
                     /locus_tag="NBI42_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1776493.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(33384..34763)
                     /gene="uhpT"
                     /locus_tag="NBI42_000027"
     CDS             complement(33384..34763)
                     /gene="uhpT"
                     /locus_tag="NBI42_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCD1776494.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            34996..35490
                     /locus_tag="NBI42_000028"
     CDS             34996..35490
                     /locus_tag="NBI42_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HCD1776495.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            complement(35849..36817)
                     /locus_tag="NBI42_000029"
     CDS             complement(35849..36817)
                     /locus_tag="NBI42_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCD1776496.1"
                     /translation="MKLGVFSVLFYDKNFEDMLDYVAESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPTEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(36872..37912)
                     /locus_tag="NBI42_000030"
     CDS             complement(36872..37912)
                     /locus_tag="NBI42_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1776497.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIEAGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGAIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            complement(37937..39013)
                     /locus_tag="NBI42_000031"
     CDS             complement(37937..39013)
                     /locus_tag="NBI42_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1776498.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAACA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRPDSQFLYKSAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLNEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHDAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            complement(39087..39926)
                     /locus_tag="NBI42_000032"
     CDS             complement(39087..39926)
                     /locus_tag="NBI42_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1776499.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            complement(39928..41196)
                     /locus_tag="NBI42_000033"
     CDS             complement(39928..41196)
                     /locus_tag="NBI42_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1776500.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYVINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            complement(41199..42470)
                     /locus_tag="NBI42_000034"
     CDS             complement(41199..42470)
                     /locus_tag="NBI42_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD1776501.1"
                     /translation="MSKILKCITLVVVMLLIVTACGPNRSKEDIDKALNKNNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLASEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            complement(42483..43580)
                     /gene="ugpC"
                     /locus_tag="NBI42_000035"
     CDS             complement(42483..43580)
                     /gene="ugpC"
                     /locus_tag="NBI42_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCD1776502.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVQPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMASDKITLAFDMNKCHFFDEKTGNRIV"
     gene            complement(43963..44541)
                     /locus_tag="NBI42_000036"
     CDS             complement(43963..44541)
                     /locus_tag="NBI42_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCD1776503.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GKGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGKQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            44750..45376
                     /locus_tag="NBI42_000037"
     CDS             44750..45376
                     /locus_tag="NBI42_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCD1776504.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPEDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAANFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            45566..46339
                     /locus_tag="NBI42_000038"
     CDS             45566..46339
                     /locus_tag="NBI42_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1776505.1"
                     /translation="MKAYKIFWLNLAAIIIISIVVSGGMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNGQDKWYIESSYSVVKSDELLKEDRMLLEVDKNT
                     HKITGDYDTTINDRKNVTHSTHKSYPIKVVNNKIVCTKDVKDPELKQKIGNYQFLIQS
                     GDLPSILNSNDLKITHDPTTDYYNLSGKLSNDNPSVKQLKRRYNIPKNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYQESLTYNKISEH"
     gene            complement(46637..48643)
                     /gene="ggt"
                     /locus_tag="NBI42_000039"
     CDS             complement(46637..48643)
                     /gene="ggt"
                     /locus_tag="NBI42_000039"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCD1776506.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPAHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHSANWATEKYS
                     RQQHARLTNYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLANYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSKL
                     IDSNKANIDIEHGVVSNCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVAGGINEIAPYKRPLSNMAPTIVMHHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIARGHAME
                     HKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDNDGF
                     RVYFNDVQLPLYAEQVRWMHGKFWIEESVIRIIFPEVSVHIEDLRSYEIAGENYIDIV
                     WLARKNAYQVALKDNGLYLNDEAYHSVKRNTNAYYRYDRDSITR"
     gene            complement(48681..50456)
                     /locus_tag="NBI42_000040"
     CDS             complement(48681..50456)
                     /locus_tag="NBI42_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1776507.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKSTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSRDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDNSIYDKLIKAYNVKSLTTDGQKVNNKGGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(50473..51636)
                     /locus_tag="NBI42_000041"
     CDS             complement(50473..51636)
                     /locus_tag="NBI42_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1776508.1"
                     /translation="MVKLTTKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHYDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGICIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(51642..53120)
                     /locus_tag="NBI42_000042"
     CDS             complement(51642..53120)
                     /locus_tag="NBI42_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1776509.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKIKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTNSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTIYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            53077..54669
                     /locus_tag="NBI42_000043"
     CDS             53077..54669
                     /locus_tag="NBI42_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1776510.1"
                     /translation="MSNLLEVNSLNVQFNYDEATVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLTNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVLFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTEASIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYKSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQSFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAFIAR"
     gene            complement(54933..56045)
                     /locus_tag="NBI42_000044"
     CDS             complement(54933..56045)
                     /locus_tag="NBI42_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HCD1776511.1"
                     /translation="MKICEKCFNNTEIVEIIANDNSKFDNCDIDNDHLGVKIFDTTKD
                     IDKLELIRDYLRPALELYDISINLPDTFSLKEGKKIEIALKDDWSIFNVEEAQISCIL
                     NELFKDDENLDRRVLEDLVGAKIINDKKYTNKNLIVANNDWDGFCESLKYKNRFHNNM
                     INLENLAFFLDITTNYYSIEEFRERFEPLYRSRIVKFITEKSELMSPPKGFATAGRLN
                     SKWISVLYLSTKEQVSIEEVKPKHNDIIYIGKVKLQKNVTKEKLKIANLTNLTSNAIK
                     AGDDGFRKYFVNHQTLKKIHKGITNPSDEQGIDYLPFQYLADYIRSLKYDGIMYESIL
                     QDGTFNFVFFDQDLFECVNYERKRVSDVKYTLTKLS"
     gene            complement(56032..56955)
                     /locus_tag="NBI42_000045"
     CDS             complement(56032..56955)
                     /locus_tag="NBI42_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCD1776512.1"
                     /translation="MYYPYLRGKQNELFAIRELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVEHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFVPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQFEELNENN
                     KYPGLGLIKRLSIMHHLEIMNRYLESQNENM"
     gene            complement(56957..57799)
                     /locus_tag="NBI42_000046"
     CDS             complement(56957..57799)
                     /locus_tag="NBI42_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCD1776513.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTMLNKLLLGRHSVNTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TSRNTLSEVQKPKENNSLLNTKAIYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPTEIKFLLYFAKMTKKDKNKLYHF
                     LKED"
     gene            complement(58004..60793)
                     /locus_tag="NBI42_000047"
     CDS             complement(58004..60793)
                     /locus_tag="NBI42_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCD1776514.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNKRINILQSFTETFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAAEALVQQATETGNNGYIWHTTGSGKTLT
                     SFKASQILSQQESIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVARHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTVAGIFTFKPNEDDRDGEVPYHSREKLEKMISDYNKKFETNF
                     STDTHNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDRSLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDADTILMRSYEEYKKEFM
                     EAYHELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKILLRLTAFDEFEFTVDEIGMD
                     AQENEDYKSKYLAVYDQVKRSTAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNREQIRRILDHADDPTLRLKRDLIKEFIDNVVPSLNKDDNIDEAYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHQLKQMIGDLPLKEKRKARK
                     AIESFVAETTEKYDV"
     gene            61006..61287
                     /locus_tag="NBI42_000048"
     CDS             61006..61287
                     /locus_tag="NBI42_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776515.1"
                     /translation="MVLITYQIILFLIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFILVHLALKLFFSI"
     gene            complement(61481..62356)
                     /locus_tag="NBI42_000049"
     CDS             complement(61481..62356)
                     /locus_tag="NBI42_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1776516.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(62359..63813)
                     /locus_tag="NBI42_000050"
     CDS             complement(62359..63813)
                     /locus_tag="NBI42_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1776517.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHNHNDSEKMDYK
                     SYAADKAKTNKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGIVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            complement(63825..64721)
                     /gene="murQ"
                     /locus_tag="NBI42_000051"
     CDS             complement(63825..64721)
                     /gene="murQ"
                     /locus_tag="NBI42_000051"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCD1776518.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAIISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            complement(64721..65776)
                     /locus_tag="NBI42_000052"
     CDS             complement(64721..65776)
                     /locus_tag="NBI42_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCD1776519.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYEHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSGLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            66362..68407
                     /locus_tag="NBI42_000053"
     CDS             66362..68407
                     /locus_tag="NBI42_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCD1776520.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNVGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGDIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRKHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            complement(68386..68486)
                     /locus_tag="NBI42_000054"
                     /pseudo
     CDS             complement(68386..68486)
                     /locus_tag="NBI42_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            68677..70317
                     /locus_tag="NBI42_000055"
     CDS             68677..70317
                     /locus_tag="NBI42_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HCD1776521.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQAGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEMPTPFEVTPAQHTDAPKYIELLTSKLQQAKQPIIITGHEINSF
                     HLHQELEDFVNQTHIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDTVSLPSLLKQLSNVSYTNS
                     ASFPVYQRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMSVEDAPKKLIDIAKAFSQQNK"
     gene            70442..70939
                     /locus_tag="NBI42_000056"
     CDS             70442..70939
                     /locus_tag="NBI42_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCD1776522.1"
                     /translation="MDYAPYTAENVVQNANKLIDIFRKNNGFIVFVRVKFYDGKDALK
                     PNSMVSLPPKEGDDYSRFHHLLDKRDGDFVIDKRQFSAFVGTDLDLQLRRRGIDTIVL
                     GGVATHVGVDTTARDAYQLNYAQYFVTDMMSAQNETLHQFPIDNLFPIMGQTVTTSDI
                     LNILN"
BASE COUNT        22839 a        13105 c        11330 g        23923 t
ORIGIN      
        1 atatttttgt attttgtatc ttgtgttttt tgaaacattt ttaacataca tatctcaatc
       61 attatcaaat tgtcatgacc attgtaatcc aatacaaaaa ccctaaggac gcttaaatca
      121 ggcaccttag ggctaactgt atctatttaa ttaagtatta ttattcgtat gtacgtaact
      181 tatgatctat caagttccac acttcttcaa catcaactgt tgtagcaaaa taagcattgg
      241 caggcttacc tgtaacatga tttaaatcga cagccatggt gccataagtt agtggacttt
      301 gctgttcaat gtcgatatta acgggtacca ttgtaaacaa ttctggttgt aacaaataca
      361 aaattgtaca agcatcatgt attggaccac cgtccatatt aaagtgagtc ttgtatgtct
      421 tcttaaagaa ttgcaataat tctacgacga actgtgcaac tggattattg atactttcaa
      481 agcgttcaat cacgtgatcg tcggctaaaa cttgatgtgt tacatctaaa ccaaacacat
      541 ttatagtaat cccactttca aaaacacgct tcgctgcttc agcatctacc caaatattga
      601 attctgctgt aggcgtccaa tttccaaatg taccaccacc catcaaagta atagattcaa
      661 tatgctcaac gattcttggc tcacgaatca atgccgttgc tacattcgta agaggacctg
      721 tcgctacaat tgttacaggt gtatcactcg tcatcacttt gttaataatc acatctgatg
      781 ctggcattgc aactgcttga cgtgatggtg tcgacggtag tttcggacca tctaatccag
      841 attccccatg tatttcagaa gcaaaggcag ctggtttaat taacggccta tccgcacctt
      901 tcgctactgc tatatcttgg cgtcccataa tatccaatac gttcaaggcg tttgtcgtat
      961 tcttgtcaac tgattgatta cctgcgactg tggttacagc taatatctct agtggactgt
     1021 caattgcccc cgctaaaatt aatgctattg catcatcgtg tcctggatca caatccataa
     1081 taatctttct tttcatttat atatccacct ttcttaagtt gttatcgata gcttatgtat
     1141 atttatttat gtggtgaatc atgtttattt tgaaaaatag ttttaacttt ctcatagttt
     1201 tggatacaaa cactatttat ctattttatg gcttataaat ttatccgata tgctttgtca
     1261 ccctacctcg ctaaaaaata ggatgtctac agatctatac cgacttttgt caactcattt
     1321 tcacaacaat ataaacagca atttatatga ttgttacatg attcaaacaa tttttatgaa
     1381 aaatattttc atccacagaa tatatattga tattaaattt cccaaaagct atattgagaa
     1441 taattaggag ggatgttgat gaaatcttta tttgaaaaag cacagcagtt cggcaagtcc
     1501 tttatgttac ctatcgcaat cttaccagct gcaggtctat tgttgggtat cggtggtgca
     1561 ttaagtaatc caaacaccgt taaagcatac cctattttag atattacctt attacaaaat
     1621 atttttacat taatgtcagc tgcaggtagt attgttttcc aaaatttacc agtcatcttt
     1681 gcaattggtg tcgcaatcgg attatctaga agcgataaag gtactgctgg tttagctgca
     1741 ctgctcggtt tcttaattat gaacgcaact atgaatggtt tattaactat cacgggcaca
     1801 ttggcaaaag atcagcttgc acaaaatgga caaggcatgg tgctcggtat acaaacggtt
     1861 gaaaccggtg tttttggcgg gattatcaca ggtattatga ccgcaatact tcacaacaaa
     1921 tatcacaaag tggtattacc accgtattta ggtttctttg gtggctctag atttgtccct
     1981 attgtcacag catttgccgc aatcttttta ggtgtattga tgtttttcat ttggccaagc
     2041 atacaagccg gcatttatca tgttggtgga tttgtaacga aaacaggtgc catcggtact
     2101 tttgtttatg gcttcatctt aagattgtta ggtccactcg gtttacatca tattttttac
     2161 ttaccgtttt ggcagacggc acttggtggt actttagaag tcaaagggca cttagttcaa
     2221 ggtacgcaga acatcttctt tgctcaactt ggtgatccag atgtgacgaa gtattattca
     2281 ggtgtgtcac gctttatgtc aggccgtttt attacgatga tgttcggctt atgtggtgcc
     2341 gcacttgcga tttatcacac agctaaacct gaacataaaa aagttgtcgg cggtttaatg
     2401 ttatccgctg cactcacttc atttttaaca ggtatcaccg aacctttaga gtttagtttc
     2461 ttgtttgtcg cacctattct ttatgtaatc catgccttct ttgatggatt agcatttatg
     2521 atggcagaca ttttcaacat tacaattggt caaaccttca gtggaggctt tatcgatttc
     2581 ttactttttg gtgtgctaca aggtaatagt aaaacaaact acctatacgt catacctatt
     2641 ggaattgtgt ggttctgttt gtattacatc gttttcagat tcttaattac gaaatttaat
     2701 ttcaaaacac ctggtcgtga agataaagtt gcagcacaac aagttgaggc tactgaaaga
     2761 gcacaaacta ttgttgctgg tttgggaggc gaagataaca ttgaaatcgt tgactgttgt
     2821 gcaacgagac tacgcgtcac acttcatcaa aatgacaaag tcgataaagt attactcgaa
     2881 agtactggtg ccaaaggtgt aatccagcaa ggcactggtg tgcaagtaat ttatgggcct
     2941 cacgttacag ttatcaaaaa tgaagttgaa gaattactcg gggattaaga ctaaccgaaa
     3001 tatcaacaga actaatggca acgatgtacg aagtaagaag tgacatcgtt gcttttattt
     3061 ttaatgttac atttgaagca ttaagttcat catgcactgt agtaagcccg caaatcgcct
     3121 ctgctagaca atcatcttaa tgctatgatt aaagcttaag tgccagactt gaatttaatt
     3181 tcaacaacga ctttcactac attaaaaata gggctactcg acacatatag ttgtatcaaa
     3241 tagcccttta tacaattttt tggggtaagg ttttacaatt tttggaatgg tatagatttt
     3301 ataaaaagtt atttaagttc ttctgcttca gccataatat cttttaatgt tttagctgaa
     3361 tgtgcaaact tgctttgttc ttcgtcgttt aatgggattt ctacgacgtt gcgaataccg
     3421 tttctattga tgactgctgg aacaccaata taaacatctt cttcatcata ttcgccttct
     3481 aataatgctg atacagtcaa tacggcatct tcatttctga aaatcgcttc agtaattcta
     3541 gctaatccca ttgcaacacc ataataagtg gcacctttag cttgaataat gtcatatgct
     3601 gcatcacgtg tttgaacaaa aatttgttca atttgcgctt tgccctcagg acgttgttca
     3661 agtaatgtct tcaaaggttg acccgcaata ttagcgtgtg accatactgg taattcagtg
     3721 tcaccatgtt caccaataat ttgagcatcg acactacgtg gcgcaacatc gaacgcttcg
     3781 cttaacaata atctaaagcg tgcagagtct aaaattgtac cagaacctat aacacgttct
     3841 ttaggtaaac cagagaattt ccatgttgca tacgctaaaa tatcaacagg atttgtagct
     3901 accaagaaaa taccatcaaa ttttgatgcc attacttcac caacaattga tttgaatatt
     3961 ttcaagtttt tagatactaa atctaaacgt gtttctccag gtttttgtgc agcaccagca
     4021 cagatgacaa ctagatccgc atcatgacaa tcactgtatt cgccagcttt cacacgaact
     4081 gttgttggag aatatggtgt ggcatgtttt aaatccataa catctcctcg aactttttca
     4141 gtgtctaaat caatgatgac taattcatca acaatgcttt ggttcactaa tgaaaatgca
     4201 tagcttgaac ctactgcacc attacctatt aatacaactt tgttcccttt aaatttgttc
     4261 attacaaaaa ctcccttatg attagttcac taacatacgt gtagtttcaa atatgttagt
     4321 ttaatgctgc ttattgacga tacaaaagca aataaacatc tcttttactt tcaacgcata
     4381 acttaaaagg tcatgtgtca tccgctttta agtttgtgat ttatttcaca tataaaatgt
     4441 aacatgcatt aagcactggg tcaatattaa attgtgattt atttcacatt ttattttaat
     4501 ttttacacct ttttaatttg tatacgatta catcttagat ttctttagtc ttcgtacttc
     4561 gccagtgatt atttacactt tcacattttt attatcatgc ttactttttt ctaagaaaac
     4621 aacaatgttt tttgaattag tcaaataaat gcgcgcaatc gtcgatgtgc aaacagacaa
     4681 ttgtacacaa cgcttattga taagtattta aaaaattaaa aatgtcatac aattatcaaa
     4741 tttgccattt tatttatatt ttctcaaacc aattaattga atatcgaaat ttttagtaga
     4801 ataatcaaaa tagacagatt aaaggaggag tatcatgctt acagaacaag agaaagacat
     4861 aatcaaacaa acggtgccat tgcttaaaga aaaaggaaca gaaattacgt caacttttta
     4921 tccgaaaatg tttaaggcac atcctgaact tttaaatatg tttaatcaaa cgaaccaaaa
     4981 aagaggcatg caatcttcag cattagcaca agcagtaatg gctgcagcag ttaatatcga
     5041 caacttaggt gtaatcaaac ccgtcattat gccagttgca tataaacact gcgcgttaca
     5101 agtttacgct gaacattatc caatcgtggg ggaaaattta ttaaaagcaa ttcaagatgt
     5161 tactggatta gaagaaaatg atcctgtcat tcaagcttgg gcaaaagcat acggtgttat
     5221 agcagatgtg tttatccaaa ttgaaaaaga catttacgat caaatgatgt ggattggttt
     5281 caaaccattc aaaatcacta acattaagca agaatctgca gacattaaat catttactgt
     5341 tgaaactgat gcatatgact ttagtcaatt tacaccaggt caatatatca cagttgatgt
     5401 ttctagtgat aaacttccat atagagctaa acgtcactat tctatcgtat caggtgataa
     5461 aaatcattta acttttggtg ttaaacgtga tgttacgatt gaccatgaag gtgaagtttc
     5521 tacaatttta catgacgaaa ttaaagaagg cgatatgatt aatttagctg cgcctgtagg
     5581 tggttttgta ttagagaata cgactgaacc acaactcttc ttaggctctg gcattggtgt
     5641 tacaccttta gttgcaatgt ttgaagctgc tagtgcgaaa ggtatagaga cacagatggt
     5701 tcaagttgct gaaaatgaag aacacttacc atttaaagac aacttcgaca gtatcgcgcg
     5761 tcgtcatggc aatgctaacg tatatacaca tttaaaagat gaacaaggct atattggtgc
     5821 tgaagaatta caagtatttt tagcaaataa acctgaaatt tatatctgtg gtggtacaaa
     5881 attcttacaa tctatgatcg aagcacttaa atctttaaat tacgatatgg atcgcgtaca
     5941 ctacgaaaca tttattccaa gactaagcgt tgcagtataa ctgagaaaag aggtttagtg
     6001 ctcatatgaa acttaatcaa cgttacgtaa aagtatttgc attatatttc gtaagtatta
     6061 ttactgcaaa tattattgtt aaaaataata atttaattaa aacattgata caaaccatag
     6121 ctgggtacac ggtctttgca gttggtttga agtatttaac taaacgtaaa aataaatgac
     6181 atctatacaa aaacagctat ggctttactt gtcgtggctg ttttttaact ttaaaacatt
     6241 tttaggataa aagaatgcgc attgaatttc ataagaaagg agtttacaaa atgaccattg
     6301 atataggacg aatttacaat cacaaagaac ggaccgatgc tattcgtgtg ttagttgata
     6361 gagtgtggcc tagaggtatg tcgaaaagta ctgcgaatct tgattattgg ttgaaagacg
     6421 ttgccccctc tactgagtta agaaagtggt tccatcataa tcctaaactt tttggtgctt
     6481 ttaaagaaaa gtataaaaaa gagttgcgtg ataatgatgc tcaaaatgct gctttcaaag
     6541 aattaaaagc tcttgttgaa aaacataatc atgttctatt gttatatgcc gccaaagata
     6601 ttgaacataa ccaagctgtg atacttcagc aattactcaa tacttaagca tatgtgtatg
     6661 gcataaatca ctatacatgc cgttaacttc atttctaata ttagattttt tgaaaatata
     6721 attactaagg ttaatagtat ttttaaaatt actaagctta agcaatattt tactttatac
     6781 aaacaaagtt ctaaaataat gtttgctggg tttaaattag taattagaaa gagagtgtta
     6841 tgaaatgaag tttaaaagtc ttgcgacaat tatcgcagca gcaactttag tattatcagg
     6901 atgtggttca acacattcat ctggcaaaga tttaaatatc tcgttacctt taaaaacgaa
     6961 gtccattgcg ccttatgaaa cagatgtacc tgttaaaatt ggtgctgcag agtccttatt
     7021 caaaacaaat gatcaaggta aaatcgaaaa agtattagta aaatcatatc atcaaccaaa
     7081 tgacacgaca ttagatttag agttaaaaga caatataaaa tttcaaaatg gacaaaaact
     7141 aactgctgaa aaagtaaaat caagtctgga aaatagcatg aagaaaagcg atttagttaa
     7201 attttcatta cctatatcat ctattactgc taaaggtcaa aaactaacga ttaaaacgaa
     7261 atcagcttat cctgaacttg tatcagaatt agcgaatcca tttatggcga tttatgacac
     7321 ggatgcaaaa tcagatgtga atcaaacacc tgttggtaca ggaccatatg aaataaaaga
     7381 ttataaacaa tcacaaaaaa tatcgttagc aaaatttaaa gactattggc aaggtaaacc
     7441 taaattagat catattactg taacgtatca agaagatggt aataatcgtg tcagaaattt
     7501 agaatctaaa aaagccgatt taattacgga tgtacccgtt aataaagtag aagatattac
     7561 gagcgataag catttgaaag tatcgaaaaa gtctggattt agaacttctt tacttatgta
     7621 taatcataca aataaaaaaa tgactaagcc cgttcgcgaa gcactagaca atgtcattga
     7681 tagacaaggt attgcagatc atatttatca aggttatgcg aaacctgcaa caagtccatt
     7741 taatgataaa ataccttaca ttaaagagcc taaattgacc aagcaaaata ttgataaagc
     7801 taaggattta ttagccaaag aaggttatac gaaaaatcat ccattaaaaa ttaaattaat
     7861 cacttatgat ggtcgtccag aattatctaa aattgcacaa gtactgcaat cagatgctaa
     7921 aaaagcaaat attgaaatcg acattaaaag tgttgatgac atagaaggtt acttaacaga
     7981 ccgttctgca tgggatgcaa cgatgtatag tttcggaacg attcctcgtg gagatacagg
     8041 ttattacttt aatcaagcat ataaaaaaga tggtgccatt aataaaggaa actatgataa
     8101 tagtaaggtt gatgatttaa ttgatcagtt gaatcatacc gttaatgttg atgaacgtca
     8161 caatatttct aatgacatta tcaaattatc tagccaagat gtgccaaata gttacatcgc
     8221 ctataatgat caaattgtcg ctgcaaattc taacgttaaa aattataaag ttactcctga
     8281 aggcatatat ttaatagatt atcaaacaac gattgaacga tagagatcct atacgactcg
     8341 cctaaacctt aaaaaaccac accatagtca ttcataatga ttgtgatgtg gtttacttta
     8401 tttttgtgat tgtattactt ttttgtttta atactccaat aatattgaat attacatact
     8461 ttcatgaaac atgttcattt taaaaaaact aattgtttaa cgtgaaacac tttttgaaaa
     8521 ccaatgttct atttcattat caagatttta attccctgac aaaccctatt tagtttgcgc
     8581 ctcttctctt tatattgatt attaatcttt tactttcaca tttactattt aataatctgt
     8641 tcattataaa aaatagctag tttcgctata acacaaaacc agccatttct cataaataat
     8701 attatttcaa tctcatagga agtcctataa atattgacca taaaatgatt tcattttttc
     8761 aaccgcttca gttacaaatg gttctttgtc atacatatca aaatgattcg caccttctac
     8821 aatgtggata tctttcggtt ctagtgcacg ttcatatgca ttttcacttt gccataatgt
     8881 accagcttca ctaccagcga taaatagtac aggttgtgtt aataatttat ctactaaatc
     8941 aaatgcagta aattcaatga tacgatcgaa actaataaat ggaaattgat taattgaacg
     9001 ttcatgttgt gctcttggcg tacgataata ttcccatcca tcttttgttt cttgatccat
     9061 atcttcagtt ggttcttctg gaacaaaacc tactagcttt tgtggtgcac catttgcttc
     9121 tgcttttctt tgttctgcaa cttgttctaa taaaggatta atgtcttttt catcttgatt
     9181 acccgtccaa cctttacgga agttttgacc gatgtcagca gcactaatgc ctactacagc
     9241 tttaatacgt ttttcagttt gcgctgcttt aattgtatag ccaccacctg cacaaatacc
     9301 aagtgcacca atacgactat catcaataaa atcaagtgta ttaaagtaat ccacagcagc
     9361 tcttacatct tctacacgtg atgatggatt ttctaaatta tgtggttgtc cttcactttc
     9421 accttgataa cttgcatcat aagccatcgt gacataacct aaagttgcta gtcgttctgc
     9481 atataaacca gctgtttgtt ctttcacgcc tcctgctgga tgattaacga caatagcagg
     9541 atattgatta ttttcatcaa aattttctgg gaaatacata ttagctacta catttaagtt
     9601 attactttta aaatttactt tttccatgtc aaaaactcct tataaccagt gtggcttatc
     9661 gccatctgta tttggtttat ttacgcctaa actttcgtta acgtggattg aaggattctc
     9721 aatgatttct aatcctaatg cagcaacact tttacgcgaa acttctgtgc ctttaaaatc
     9781 ttcgccttta attgttgttt catattcaat ctcatcgtta ttcgtcaacc atgcgggacg
     9841 taatactgta tagtctaagc tagatgcttc aataatttct gctgctttct tatatggtgg
     9901 aagatatggc ccaatattgt cattgttcca ttttccaaat gcgccaggca cctcatcata
     9961 tatacctaat gcagttacaa aaataagtcg ttgcacattt ttcttttcca ttgctgatac
    10021 aattgcatga gcttgttcat ctatattacc ggctaaacta gcgaatacaa catcaatttg
    10081 atccaatgca ttttccaaag cggcttcatc tgttgcatct gcttcaatta gtgttacgcg
    10141 ttttccatct tctatattca tcaattttgc tttatttctt aagtaaagtg ttaaatgatg
    10201 ttctgtttta tctaataata agtcaattaa ctcttgagaa atttgaccac ttgcacctat
    10261 gattaaaata tttttctttt ccaactaaca tcacctcttt tattactcac ttgtgtttta
    10321 aaaaacaagt gttacactaa tatatacctt catctatcat ttttaaactt aaaaggtaat
    10381 gaaaaaagac ttattgcaca ggtacttaat tttcgagcaa tatttaggag ctatatcatg
    10441 gataaacgaa ttaaaaaaac acaacgaaca ttgaaacaaa caatgattac gttgttaaaa
    10501 gagcaacaca tcgaagatat cacagtgaca gatatatgtg ataaatctga aattgttaga
    10561 agaacctttt acgcacattt caatgataaa tatgatttaa ttgaacatat tatgagcgat
    10621 tatatttcag attttgtaga gatatgtcat ttcaaggagc aaataaattt taaacttggt
    10681 aatattaagt ggtttgaata tttatataac aataaagaca ttttcagtgt tttattcaaa
    10741 agttcaattt cagcactatt cagtaaaaaa ctagagacgg ttatttacaa acaacttgaa
    10801 aataaaattt ctgaaagcgt cattaatgaa ggtataagta aatcaacatt attatgtttt
    10861 ttaacttcgg ctatactagg tgttatggtg aattttgcat taaacggtga ttcagattat
    10921 caaaataaag ctgatgaaac cttattacta ttaaaacctt atttaaagga tgacatactc
    10981 aactcgtaag tatcatgctt ataactatga tgacatataa atctatatca tttaatttta
    11041 cacttcgtat taaaattttt acgacgtacg aaaaaagctt atgtcgctat aacacatagc
    11101 aacataagct ttaattatta ttcgtctact tttttccgtt gttctttgaa ttctttttgt
    11161 agtgtattca cttgtttcaa acctgcaccc attaaaatga atacgagtat ccatacgact
    11221 acgattaaaa tgaatatttt taaagtatca ctgccaagaa tcgttaatga tgtatcccaa
    11281 atgccatgta acccaacagc tgataaaaag aatattaaaa atcgtttatc taaaatatct
    11341 ttaaactcga aaccatgttg ctctttagca ataacaatag cagccccaac aatcgctgac
    11401 caaactaaat gaccaccaat cgcagtccac gcacgtgtga agacaatatc taataatggg
    11461 acattttctc ctaaagcgaa attcaaaata taacctgctg attcaaaaac tgcgaaccct
    11521 gcaccaatag cagcaccgat taataatcca ttcaaaatct tatttgtttt caatttattg
    11581 acgaaataaa caataatgag tgcttttcca gtttcttcaa ctaatcctac taaaaatgca
    11641 tcgaaaaatg ttaaagaacc aaacctttcg aattgatcac taaaaacgac aaatctatat
    11701 aataccatcg tacttagtaa tgaaaatact ccgccaataa agaacatgat aataacttca
    11761 aaaatgctaa tatttttaaa cgcatttgat tcataaaaga agaacaaacc cgataatggt
    11821 actgttaaag cccctataaa aatgagcccc ggaatcgcgt tagtgttatt aaaaattgct
    11881 gccatgaccc ataatccaat aaatgttact gtgaaagcga tgaatactcg agaaaagagc
    11941 catggcttcc cccattcttc tgatatgtca caaattgctg gcgtagtttt agcagtaccc
    12001 gctataaata tttcatctgc ttcgttttta gtatgtggct taaatacttc agaaaacata
    12061 tcttttaaat taagatttaa gctttcatca ttgcccgcta gtctaccaat tgatttggtt
    12121 gcattatcaa ataaacttct tgcttcgttt tttaaatctg cattttgcgc tgtttcatgc
    12181 gctttggctt ccatttgttt atcgatagat tcattatgta tgtcggacgt cttagttgat
    12241 tgtgtaattg ctgatgacat cccctcttca cttgtattag gtgcaactga ttgcgtttgt
    12301 gttatctctt ctttttctgt atttcttcga tgattaagac cttccataga cttacctact
    12361 ttcacttttc ataataaaac tattttaatt taatattgat ggattttcca tattttaata
    12421 taaatacttt gttcgattgg gcgctttgta ttccatattt gaaaatcaaa acctatttaa
    12481 atttttatat tccgagcaat atttttatat aagtatttta atgatattca attcattaca
    12541 aacccttgat attacaattg ttatcgacta ttaaaatgtg tctatactgt gtctattatc
    12601 agaataaaaa gatatttcta attgatttta aacgccgtta tgttatattt cttgtaaggg
    12661 tttacacaaa gtgtaaaaac gctatatttt atcaaatgtg atggaggtga cttgcttgaa
    12721 acaaatcaca tggcacgact tacaacatat cattaaagat ggtgatgtga ttggtttacc
    12781 agcattagct gtagccaact tacccgccga agttctacgt gctgtgttag cgcaacatga
    12841 cacatatcat acgcccaatg atttaacgtt tatattagcg aatgatatcc atagtttagg
    12901 tgccgcaccg gatttagatg attttataga acgtcgtatg attaaacgtg tcattatgag
    12961 cattttaacg gcttcttcca aaacggcaca agcaatgaaa aataatgaca ttgaagctta
    13021 ttttttacca caaggtatca ttgcaactca ttatcgtcag agtaatcaat tattacctgg
    13081 agttattact aaaatcggat taaacacagc tgttgatcct agatacggtg gcggtaaagt
    13141 aaatacacga acaactgatg atttagtttc attagtaacc atcaacgatg aaacatactt
    13201 actctacaca ttccctagcg ttgatgtggc actactgaga ggaacatacg cagatcaaca
    13261 aggtaacatt tatttaactc aagaagcgta cttgagcgag tgttatcatg tcgcattaaa
    13321 cacgaaagcc aatcatggga aagttattgt gcaagttaaa gctttagttg acgactatca
    13381 actaaaaccg aatgaagttg ttatcccagg aaatcttgtc gattatgtat acgtcacaga
    13441 agatgaaaag aatcaccgcc aagtaattca aagtcattat ttaccagcct tgcctggaga
    13501 agaacgaatt gatggaatac ctgaacccgc attacctttt aatagtcgca aattgattct
    13561 ccgacgtgct gctcagtttt taacttatgg cgatacaatt agcatcggtt atggcatcaa
    13621 taatgaactc tctaatttat tgcatgaaga atgtgttgaa catgatgtgc aaccgatttt
    13681 agatgttggc attttcggtg gattcgttgg gagtcgtgaa cattttggta tgaattacaa
    13741 tgcagatgtg cgcatgcctc atgatcgagc atgggatttt atttataaca atggtgtatc
    13801 agttgcctat cttagctttg ctgaggttga tcaatacggc aatgtcaacg tgtcttactt
    13861 caatgaccga ctaaatggat gtggcggctt tatagacatt acacaatctg taaaaaaaat
    13921 tatcttttca ggtacttttg tagctggcag tcatgtctca tgtcataatc aacgattaaa
    13981 cattgaaact gaaggacaaa accagaaatt tgtatcagat gtgagccata tcgactttaa
    14041 tgcacaatat tcacaatcac tcgagcaaga agtctatttt gttactgatc gtgcagtatt
    14101 cgagctcacc aatcaaggct tgaaactaat tgaaattgca ccaggtcttg atttgcataa
    14161 agatattttg aatcaaatgg cttttaaacc aattattgct gatcatttaa aattaattga
    14221 taccagcatt tacaaagaaa aatggggaca acttaaacaa tcaattcata aagtatgaaa
    14281 gtgaagctaa agggggtata ctaatgaatt tcgattggat aaaaacgcgt tcagacttcg
    14341 atgatgacaa gcctgccgtt attgatcacg caaaacaaac atcttggaca taccaacaac
    14401 tcaatgcacg cgctgataat atggcacatt atttaacatc tcaaggtgtt aaaaaaggcg
    14461 atgttatcgg tatttttgcg ccaaatgata ttgcaatatt agatttattg tttgcttgtt
    14521 ttaaaacagg tgcggttttt ttaccattga attggcggct taacccaaaa gaaattgcag
    14581 ccattgtgga agatgcacaa ttaaaactgc tcttctatgc tgaaaaacat ttaagttcac
    14641 tcatcgatat tgaccaaaac ttattgcata tggatattga tgtagcgcaa tatgatgaaa
    14701 tcgtaaatcc agactatcac caaccttttc aagcaacacc tgttgaacca caagatcttg
    14761 cagcattaat ttatacaagt ggtacaactg gatcacctaa aggtgtgatg ttctcatatg
    14821 aatcatttgt tcataacggc gcaaacttag agctgacgta taagttcaat tcaaactata
    14881 ttacgattgt atcaacacca atgttccatg ttttagggtt taacgatact gtattaccag
    14941 tattgatgtc aggtggcaca cttattcttc aacgttactt taacggtgaa gaattgaatg
    15001 acatgattgc acaatatcac cctacattta ttatcatgat tccgacaatg tattatagta
    15061 cgctacgtgc tagtaatttt aatcctgaaa attttagagc tatggattat atcatccaag
    15121 gtggttcaca accattacca agtatccaag cagcttttaa acaatatggc ataaacatta
    15181 ttaacggcta tggtttaact gaagcacctc ttgtacttgt taatacaccg gaaaattcaa
    15241 aacgtaaacc aatgagtatt ggtaaagcag tcatgtttgt tgatgcacgt atccttgatg
    15301 ataacggtga agaagtacct actggtgaga ttggcgaact tgcaattaag gctaaaaatg
    15361 tcacgccagg atattggaat aaaccagcag agactgccaa agcatttcat ggtcgttatt
    15421 tattaactgg tgacttagcg aagatggacg acgatggcga tatatttatt attgaccgca
    15481 aaaaagaatt aatcataact ggtggcgaaa atgtcttacc atccgaagtc gaaaatgctt
    15541 tagctgagca tccactagta gaccggtgtg tggtcgttgg ctatgatcat ccaaaatatg
    15601 gtgagtcaat tgctgcagca attatacttc gcgaagatga acctcattac gctgaaattt
    15661 tagatcaaca tatgcgaagt catttagcag gttataaagt tccaagaatg tatgtaccag
    15721 tgacacatat gccgttaaac agtacgcaga aaccagataa acttgcgatt cgacaaatga
    15781 tgaatgacaa agtctcacaa acactttaaa ggtggtaaaa atttttgaca tttagtgtaa
    15841 gcgtttacat gtgaagcgtg ttgtttttga attaaatgca tttcacatta gtattcatat
    15901 tatttttagg aggaatttat atgacatttg aaaaagaaac agtcttaaaa acattatttc
    15961 ctgaagatgt acttagtatt gctaaaggtt taacagacgg tgaagttgaa tttttacaac
    16021 aagtagattc attgctagaa agtaagtacc gtgaaaatat taatcaacat tggatagacg
    16081 ctactgtacc cgaggactat tttaaagatc tgggagaatt aaattatttt aacaatccat
    16141 tgctatacaa ggattgtcca aacgccaaaa tgcctagtca actatttcag tttttcatgt
    16201 cttacctact cgcgcgattt gatatttcct tagctaccct gctcggtgtt caccaaggtt
    16261 tagggcataa cactttctat ttcggaggta gcaaagaaca aattgcgaaa tatgtaccta
    16321 aattacagtc acatgaactg cgtacatgct ttgctttaac tgaaccagaa cacggttcgg
    16381 acgttgcggg aggtcttgaa acagtcgctg aacgccaggg cgatacttgg gttatcaatg
    16441 gtgaaaagaa atggattggt ggtgcacatg tatctgatgt cattccagta ttcgcagtaa
    16501 ataaagaaac tggcaaaccc cattgctttg tagtcagacc agaacaagat ggcgtcgata
    16561 ttgaagtcat tgataataaa atcgcacttc gcattgttcc taacgcacta attaaattaa
    16621 ctaatgtcaa agtagatgaa gcggatcgct tacaaaacat aacaagcttt aaagatattg
    16681 ccaaaattct ttattcaacg agagcaggcg ttgcttatat ggctacaggt ggtatggctg
    16741 gcgctttacg cgccacatta gattatgtca cagagcgtaa gcaattcggc aaaccaatta
    16801 gtaagtatca gctaatacaa gaaaagctag caatgatgca aggtaattta gctcaagcaa
    16861 tggcaacatg tgctcaatta gctaatatgc aagcacatgg tgaatatgac gaggttgcaa
    16921 cttcaacggc gaagatgatg aatgcattac gtttgcgtga gacagtagct atgggccgcg
    16981 gtattacagg tggtaatggc atactagctg acgattatga tattgcacgt ttcttctctg
    17041 atgcagaagc gatttacacg tacgaaggta cacatgaaat taatgcctta gtaattggac
    17101 gcgctttgac tggagattct gctttcgtat aaatagcaaa gaattatatg agatgcagta
    17161 atatcactaa aaaagactta ttttaagcat aaagcttttt ccttaaatac gaggctaaga
    17221 tgactgtcaa agatactcaa tcaattttat aaaatagcaa cgttattcca attatcttaa
    17281 tggttatctt atcctcaact aaattggagg aatcactatg acaattaata aagtaaccgt
    17341 tcttggcgca ggcacaatgg gcgctcaact ggcagcactc tttgtgaatg ctggacttaa
    17401 agtaaaacta ttagatatta tagtggacaa aaacgatcca aatctcattg cgaaaaaatc
    17461 ttacgataaa attacagata agaaacggcc gctactattc gacttaaatc tagcgagtca
    17521 tttaacatat ggtaattttg atgatgactt ggtaaatgat gatgctgatt tatatatcga
    17581 agcagtcaaa gaagatattg aaattaagca tgctgtttgg caacaagttc tacaacatgc
    17641 taaagaagat gctttattcg ctacaaatac atcaggtatt ccaattaatg cgattgctca
    17701 agcatttaac gagaaggatc aagaacgatt ctttggtcta catttcttta acccaccacg
    17761 tattatgaaa ttagtggagt taatacctac gtcacacacg aaggaatcta ttatattaga
    17821 tgtaaaaaat ttcgcgcaaa atgtgttagg taaaggtgtc attgtcgtca atgatgtgcc
    17881 tggctttgtc gcaaatagag tcggcacgca aacaatgaat gatattatgt atcgcgccga
    17941 gcaacacaag ttaagcattg tagatgtgga tgctttaact gggcaagcga ttggtcgtcc
    18001 taaaacaggt acatatgccc tatctgacct agtcggttta gatattgcag tgtctgtaat
    18061 taaaggcatg caacaagtac ctgaagaaac accttatttt catgatgtca aaattgtaaa
    18121 tacgttgttt gaaaatggcg cactcggacg taaaacgaaa caaggatttt acaaaaagga
    18181 taaagaaact aaagctcgac ttgtttacga tgttgaaaaa caagattatg tacctgtatc
    18241 gcaaccacaa ttaccaattt taaatgaatt taataaagac ttagtgcata accttgatgt
    18301 catattcaat gcgcaagacg aagcgggact atttttatgg gagacattac gtaataattt
    18361 ctattactct gccatcaatg tacctaaagc taccgatgat ttccgagaca tagaccgtgc
    18421 gcttgtctgg gggttcaact ggaaacttgg tccattccaa ttatgggatg caatgggata
    18481 tgaacgtgtt aaaacacgta tggaagacga acttggagac ttaccacaat ggattagtga
    18541 tttagatagt ggcttttata aacaagatga gaccatcgaa tatgcaacac ctgtttctca
    18601 cttcgtaaaa gatgaacttt gggataaagg tgatgccaaa ctttccataa ctcatgatga
    18661 tcaactgtta ctgaaattac aaagtaaaaa taatgtcatt accgatgaat tcaacgatgc
    18721 gttagttgat gcgattgatt tactggaaaa tgaacattac acaagtatgg ttatttatgc
    18781 agatggtaat aatttcagtg taggtgctaa tcttttctta atgaaaaagg cgcatgaaga
    18841 cggtcttgta gatgatgtcg ttgcacaatc aattgataaa ttacattata gctttaatcg
    18901 tttgaagtat agtttgaaac caatagtcac agctgttcaa ggtcgtgcct taggcggtgg
    18961 ctgtgagctt gtactttact cgcctatcgt tgtagcagca agtgaaacat atatcggtct
    19021 tgttgaagca ggtgttggct tattaccgag tggcggtggc cttgcagaaa tggctgatcg
    19081 catattatgc acatcgcata agtttgatga caaacaagct tccatgacaa aagtactgac
    19141 gaatatcgca tttgcgagag tctctacaaa tgcctttgag gcacgtcgtt atggttattt
    19201 acgtgataca gatacgatta ttttcaatac agcacaacgt gtcgaagttg cactcaaacg
    19261 tgcgaaatat gaagcagaaa caaactatat acctaaacca aaatacaaat atatcggctt
    19321 aggcgaagac ttcaaagcat tgatccaagg acaattagat gcgcaaagac ggggtcattt
    19381 tattagcgac catgattatc atatcgcctt aaatatcgcc acaattttag cgggtggtga
    19441 tttaccaaga aacacattta tcaatcaacg ttacattcaa tcgttggaga aaattggctt
    19501 tattgactta ctaaaatcta aaaaatcata tgaaagaatt gcgcatatgt taaaaactgg
    19561 taagccatta cgtaattaaa agatagtcat taagagagga tgataaccat gcaagaagca
    19621 tacattgtag cttatgggcg ttcagccgca gcgaaagcaa agcaaggcgc attattccac
    19681 gaaagacctg atgatgtcgc agccaaagta ttacaaggcg tattgaaacg tattgacgga
    19741 aaattcaata agaatatgat tgaagatgtc attgttggta cggcttttcc agaaggatta
    19801 caaggacaaa acattgcacg aacgattgca ttgcgtacgg gattatctga cacggtaccg
    19861 ggtcaaacag tgaatcgcta ctgctcatca ggattacaaa ccatcgcgat tgcagccaat
    19921 caaattatgg ctggtcaagg agatatactt gtagctggtg gcgtggaatt gatgagtgcc
    19981 gtaccaatgg gtggcaacga gcccacaaac aatcctacct tacaatatga tgatataggt
    20041 gcgtcatatc ctatgggttt aactgctgaa aatgtagcat cccaatttga cgtatcacgc
    20101 gaagatcaag atgcttatgc tgtcagaagt catcaacgtg cctatgacgc ccaacgtgat
    20161 ggtcggttca aaaatgaaat tattccaata caagtaaact cagttgaata tacaaacgca
    20221 ggaccaaaaa tacacacaaa tatctttgac caagatgaat ttatacgacc tgacaccacg
    20281 atggaggcat tagccaaatt acgtacagta tttaaagctg acggcactgt gactgcggga
    20341 acatctgccc cactttctga tggtgcagga tttgtagttt taatgtctgg agataaagtg
    20401 aaagaactag gcgtgacacc tattgcacga ttcgttggtt ataaggcagt aggcgttgac
    20461 ccgaaaatta tgggtattgg gcctgcatat gcgattcctg aagtattgtc actcagcaat
    20521 ctatctgttg aagacattga tttgatcgaa ttgaacgaag catttgcttc tcaaacgatt
    20581 gcttctatta aagaagtagg tctagatata tcacgtacga atgtgaatgg tggcgctatt
    20641 gctttaggtc atccattagg tgctacaggg gcaatgttaa ccgcgcgttt acttaatgaa
    20701 atgggtagac gtcccgatag ccgttacggc atggttacga tgtgtattgg tgtcggtatg
    20761 ggtgcagctg ctatatttga gtatgtgcgt tagaatggtt gactttggat gaagcggatt
    20821 cgtttggtta ttgaatgaag taggctgaag ttgaagcgga ttgaagcagt ttcgttttat
    20881 tattgaagct atgtgaaata tagtgattga accaaatagt ggtttaatag gatagcgggt
    20941 atttccgttt tagaatttaa catttacacg tctaatttta atcattgttt taaattttat
    21001 gaatcgaagc cctttgattt aataatattt gctaatacta gtatcttatc tgattgttca
    21061 tgtttaaaat aaagaaaacc actcacatca gtgtgtgttc gaactagact tataagttcc
    21121 agttcggtac gactttctaa agcaattatt attgctgtga ttgtcgtata tcacatagat
    21181 gtgcgtggtt tattttaata ggttagtaat atattaggtc atgttatgtt taagactata
    21241 atgaataaat aatttagaaa tatgcttccg attgttcgat gctttaattc agttagaagc
    21301 atcatcgaat gcatgattac tgttgtaaag atacataata ttttgtattg actgtatgtc
    21361 tatggataga gttataaact tattttgtta ctctaggccc atatgtcgca gtaccatctg
    21421 catgtgttgt tacattgtat gcatttgttt cgcttggctt cttgtatgtc ggacgagctc
    21481 catatgatac ttggccgttt gcatgtgttg ttacattata ctcgtttgtt ttgcttggct
    21541 tgttttgtgt cgggcgagcg ccatatgata cttggccgtt tgcatgtgtt gttacgttat
    21601 atgcatttgt tttacttggt ttgttttgtg ttgggcgagc accatatgat acttggccat
    21661 ttccgtgtgt tgttacgtta tatgcattcg ttttgcttgg cttgttttgt gtcggacgag
    21721 cgccatatga tacttggccg tttgcatgtg ttgttacgtt atatgcgttt gttttacttg
    21781 gcttgttttg tgtcgggcga gcgccgtatg atacttggcc atttccgtgt gttgttacgt
    21841 tgtatgcgtt tgtttcactt ggcttattga atcttggtct cgcttcatat ccaaatgttc
    21901 catcgttgta ttcacggata cctgtaccag catctctata tttaacatat ttaggtgttt
    21961 tgttaaattg aggtctcgct ggataatgtg atgcttctgt tgtttcagat gcttgaggtt
    22021 taacctcaat atcacttgat tctccttgaa tacctttcaa cgttgattca gtaccttgtg
    22081 gttttatttc aagtttagat gagctacctt caagaccttc taaaataggg ttcgttaacg
    22141 ttgggtttgt ataattatta cttaaagatg gtctgttttg ttccattgtt gggaaatcag
    22201 gaccttgaac gatttcacct tgtaacgttt tattttccat agttggatag tctggacctt
    22261 ttacagtttc accatagatt gttccttgtg gaatttttac taatggctgt gccactggtt
    22321 gtgttgtttc atcagcttta ccagcagtag ttttaacttc ttgttggttt ccaactttag
    22381 gtaattgagg ttctttaact ttcttctctt cttttacaat aggagatttt gttacagttt
    22441 cctcgtattt tttgacagtt ttatttttcc aagattcgtc tgcttcttta actgcttttt
    22501 ttgtttcttc aactaattta tcaaaattag gtttattttc actcttctct ttaaaattgt
    22561 gttttgttgg atcatatttt gttatagaat tcggtctatt ttgtttagtc tccataaaga
    22621 aatcatcaat aattgaattt aagtcatcga tcatttcttt ttttatacgc tcatttgtaa
    22681 ctttatgtgg attgtctgta tctccaagga ttaagtccag ttttgctcgt aactctttcg
    22741 cgtgctcccc ataatcctta tcagcataat aagttacaac taatgtatca atttcagaaa
    22801 caaggtcata aacttcttta gttgccttgt cttctccatc tttatcaaac tgcttcaagt
    22861 caacattttt atgctctatt tctttaactt ctttattaaa ttgataaact gcatctttca
    22921 aagagttaaa aatatcgtta tattcattca ttgttaaatt gtaaagatta tatttatgga
    22981 aagtaagcat attagtgttc ttatttgaac ttttatacca ctttttataa agctcatatt
    23041 catctatctt tttctttaat aaatactggt cttctcctaa tactcttgtc atcaatctat
    23101 ctttcgcttc tttatagata gaatctccat atttataatc ttccaataag tctattgctc
    23161 cagtaaattg tgcctctaaa ctatcaatta ttttccaata gtaataatct gaaacagtag
    23221 ctcccttttt actgttctca ttcactcttg attctttact ataatccttt gttactatcg
    23281 catctgcttt gttatcccat gtaaataagc tagatgcaac tgctaatgcg cctagcgaaa
    23341 ttatttgctt tttcataatt ttttaattcc tccaaaatgt aattgcccaa tctacattaa
    23401 agaaacaaaa tattaaaaga cattaactat atattaacta gaataaacaa agcattaact
    23461 atctttttgt tatagttaat tagctttgca atatacaata gtgtataatt tttttgatta
    23521 atgtacatca aaggagtaac aaagcatgac aacacaaatg aaaatcaaaa catatttagt
    23581 tgctggtatt aaagcggcgc tccttgatac gactggtatt aaattagcaa gcaaatctga
    23641 aactacatca catacgtatc aacgtcaagc gcttgtagat caattacatg aattaatagc
    23701 aaacactgac ttaaataaat tatcgtgcct aaatttagat gcgtatcaaa aacgcgatat
    23761 tttagctgcg cactatattg ctaaatccgc tatacgcact aaaaatttgg atcaaatgac
    23821 taaagcgaaa catagattag aaagtattta cgattcaatt tctaaccctt tgcattcaca
    23881 aaacaattag caacttatca ataaatcatg taagtgtttg tgacgccaaa ttgccttaca
    23941 aatccttgtc atatgattat aaacgaatga aacgattgcc taccccatag aaggcaacgt
    24001 taaaaagacc tctgaaggta tcatcttgat aactttagag gtctttgttt atattattca
    24061 aataatattc ttattgaacg atcgaagtat gtttcgtctt tcttttcttc tttaatctga
    24121 tcagctaatg ctgggtcatc tgtgataata ccatcaacat tggtttgtaa gtatttcgtt
    24181 aaatcttctt cgccgttaat ggtccaagta tagacttctt tattttccaa gtgtgcttga
    24241 ttaacaagtc ttggcgaata agaaaaatct tcgatgacaa agaaatctaa tgatgtttct
    24301 ttaaaatgac caaactgcaa cggaatgata taaccacact tgagatatgg cgcttctttt
    24361 ttcaacttag tcatcacatc ataatccaat gacatcacac gatattgatg ttcaacacca
    24421 tgctttttca aaatatcaat aacacgttgt gtataatctg ctggttcttt accatgtggt
    24481 tttaactcta ctagtagctt cacatttgat tgtttagccg tttccataaa ttcgtctaag
    24541 gatacaagtt ttgcttcacg tccattttga cgcattttca aaccgacgac atctttgaaa
    24601 ttagattcag aaatattttt attaacacct gttaaacgtt tcaaattgtt atcatgacta
    24661 acaacaaatt gtttatcttt cgtcataatt gtatctaact caacgtattc gacattcgct
    24721 tttgcagcag ctttcaatga cggaatagaa ttttcaacgc ctttatcttc gaaaccacga
    24781 tggccaataa tggagatatt tgtattgata gtactattgt aaagtaaata catgttataa
    24841 ccgataaaac atgtcaccgc taacaacatc gatacaatga aaaatttaga cttacgtttt
    24901 ggttttgggt atttaaattc taatcccggt tgatctaata cattttcttg ttttaagtga
    24961 agtactaaca cactgattaa tgataatttc gtaaataaat aatagaagaa taaagcgctt
    25021 ttcagtacaa caaataaaat ggatgatact aaaaacttat ctccttcttc atctacataa
    25081 atggctaaaa aagttgcccc ggaaataatt aatgttaaaa tcgcaccaat gatgagttct
    25141 aatataacta tttctattac tagtcgaaat ttattccgct tcgtaatttg ccagctcaat
    25201 ctcatatttt taaataatga ttgcctattt aaaatcgtca gcggtaaagt gaagattaat
    25261 ttaaagttta atataaatac agcaaccata aaggttccgt atatgataat cccctttgtc
    25321 gttttcataa gttcttctgt taaaaattta ggtatgtaaa tattttttgt taatactgaa
    25381 cttagtccta ggttggcaat gggtatcatt aacattaaat aaatgacaaa gaaaataact
    25441 ggtacaccta tgagtttacg cacatttaca aaggcatttt taaaaatgga tttaaatgta
    25501 ataacctgtc gatcaaagcc ggcataaacc atataaacta acaatgaaaa ctctacataa
    25561 atcagaaaag caacacttaa tatgaatata ataagaagta tcacactggc aggatgactt
    25621 acgatttcca tccaattgtt aatcgtaagt tggctttgcc cagctacttt taacatcata
    25681 ttaaatagta aaattaagta tgtactacta ataaaaatca tgattaactg caatagtaag
    25741 gcattaatgc taaaacgccc tttattttga tacagtaatt taaatactgc ccatatatct
    25801 ttactaattc tcttcataat cacgctccgc attgctttaa tattaagttt catcttaata
    25861 tttttcatta ctcagggtca ataaaaattt gaaaagactc atattcatat gcaagtagca
    25921 attaataacc cattcgatga tattggaacc ccacctttaa caaggcatca cattctttat
    25981 cagcatagct acttacaaaa acgtctcctt caaccattgt agaagttgtc tcatatacct
    26041 actacgtctt gctaatactc tatactcaat tgaaaaagaa gcatatgccg cttcactctt
    26101 gaagttgcat atgctttctt ttcggtctga attgtattta taattcaacg ggaattttcc
    26161 ctttgaagtt aacataacgg taggctgctt taacagcttc atcatcgggc gcttcgacat
    26221 cttctaattc atatgcaatg cccaatgttt tccacttatg aacacctaac tgatgatatg
    26281 gcagaatttc aaacttttcg acgttatcaa gagaattaat aaattcccct agtttaatta
    26341 aatcgtcttt atcatcagaa taaccaggca caaggacatg tcgaatccat acaggttgtt
    26401 tcatatctga cagtttgcgc gcgaagttaa ggatgtgtgt attaggcttt cctgtcaatc
    26461 taatatgttt gtcattatca atatgtttta tatctaataa tatcaagtct gtatgttttt
    26521 gtaattcttc aaaatgcctt tgaaatgctt ttgtatcatt agcacatcca gccgatgtgt
    26581 ctaagcaagt gtgcacacca ttttctttta attctgcaaa taatttttct aagaatggca
    26641 tttgtaacaa tggttcgcca ccactgactg ttacaccgcc acccgatgca tcaaagtatg
    26701 gtttgtatgg taatatttca ttcaccattt catcaactgt gacttctctt gatggctcac
    26761 taattttcca agtatctgga ttgtggcaat acaagcatct aagtaagcat ccttgtgtaa
    26821 ataatatata tcttaatccc ggtccatcga cagtacctaa actttcgaca gaatgtaagt
    26881 gtcccttaag catagtgctc ccaccttaaa ttttgttaca tactttcatg gaatgtacga
    26941 gaaattacat ctaattgttg ttcacgtgtt aatttaatga agttaacagc gtaaccagat
    27001 acacggattg ttaactgtgg atattcttct ggatgttcca ttgcatctat taatgtttca
    27061 cggttaaata cgttaatatt taagtggtga ccacattgca ttgcgtaacc gtctaacata
    27121 ctagttaagt tacggttttg atcttctggt tctttaccta gtgattttgg tacgatactg
    27181 aatgtatttg aaataccatc tttacagcaa tcgtaaggga tcttagctac agaacttaat
    27241 gaagataatg cacctttttg gtcacggcca tgcattgggt ttgcacctgg tgcaaatggt
    27301 tcgccagctt tacgtccgtc tggtgtgtta ccagttttct taccgtatac aacgtttgaa
    27361 gtaattgtta atacactcat tgtatgttct gaatcacgat atgttttatg actacgtaat
    27421 ttagtcataa agcgttctac taaatcaact gcgatatcat ctacacggtc gtcattgtta
    27481 ccgtatttag ggaagtcgcc ttcgatttca aagtctacta caagaccttc ttcgttacga
    27541 attggtttaa cttgtgcata tttaattgca gatagtgagt cagctgctac tgataaacca
    27601 gcgatacctg ttgccattgt acgtacaatt tctgtatcat gtaatgccat ttcaatacgt
    27661 tcatagctgt atttatcgtg catgtagtga ataacattta atgagttaat gtaaacacct
    27721 gctagccaat ccatcatttg atcaaatttc ttgaatactt cgtcatattc taatacttcg
    27781 ctgttaatac cttcgaagtt tggaccaact tgtgcaccag atttttcatc tttaccacca
    27841 ttgatagcgt aaagtaatgt tttagctaag ttcgcacgtg caccgaagaa ttgcatttgt
    27901 ttaccaattg tcatcgctga tacacaacat gcgataccat agtcatcgcc atagctttca
    27961 cgcataatgt catcattttc atattggata gaacttgttt taatactcat ttttgcacag
    28021 tatgttttga agttgtcagg taaacgtact gaccataata ctgttaagtt tggttctggt
    28081 gctggaccta agttatctaa tgagtgtaag aaacggaatg agtttttcgt aacaagtgga
    28141 cgtccgtcaa tacctacacc accgatagat tcagttaccc aagttgggtc tccagagaat
    28201 aattcattgt aatcaggtgt acgagcaaat ttaacaatac gtaatttcat gatgaagtgg
    28261 tcaataattt cttgaacttc gctttcagta ataacacctg ctttaaggtc acgttcagca
    28321 tagatatcta agaatgttga tgtacgacct aaactcattg ctgcaccgtt ttgttcttta
    28381 attgcagcaa ggtatgctaa gtataaccat tgaactgctt ctttgaagtt ttctgctgga
    28441 cggcttaaat cgaaaccata tttttgtcca agttctttta attcttttaa tgcacgatat
    28501 tgttctgata attcttcacg taaacgaatt acatcttctg acatttctgt agacatcgtg
    28561 ttgaagtcat gcattttttc ttccattaag aaatctacac catataaagc tacacgacga
    28621 tagtcaccga taatacgtcc acgtccatat gcatcaggta aaccagtgat tacacctgct
    28681 ttacggcagt tcaacatttc tctagaatat gcatcgaata caccttggtt atgtgtttta
    28741 cgataatctg taaagatttt ttcagtttct tcgtctaatt cgtaaccgta agcttcacaa
    28801 gctgctttcg ccatacgaat accaccgaat ggttgcattg aacgtttgaa tggcttttca
    28861 gtttgtacac ctacaattgt ttctaaatct ttgtctaaat aaccagcatc atgagatgtg
    28921 attgttgaag ctactttcgt gtccatatcc cacatgccgc cacgttcacg ttcttctttc
    28981 gataactgca ttacttgttc ccaaagttta gaagttgctt ctgttggtcc tgctaaaaat
    29041 gaatcattac cttcataaag agtgtagttt aattggataa actctcttac atctacgtgt
    29101 ctgttccatc ttccattttt aaatccttgc caagctgttg catgattttt atttgtttct
    29161 aacatactaa acgcctccat ctgtataatg ttgtgaattt gaaatgctat gttaacgttt
    29221 tcatagacat tgtagcatgt ttgttgtgat ttttttcaca taaattatgt acaattttca
    29281 tcaaatgtat gtcaaaacat agaaactgta ctaatttgtg taacagtaat atgctaattg
    29341 cattctgtta cacaacagtt ataatgttca ctattttcat tgctttaaaa gttaatttag
    29401 gtggatatat ttgtttataa aaaatgattg ctaataagcg ttaaatcaat acttagaaag
    29461 atttactttt atatatgaag agacaggatt aaatactttt attgacgtaa aaatttactt
    29521 ttgaaccgtt caatatcttg ccgattttta tataacagct tcaaataaaa tataacagtt
    29581 tgactttaca gcctcggtaa gtcgtcttga caaacaaaaa ttatgtgcta tcacaacatt
    29641 tgcaacgtct taacaagtca tctataaaca tttctaaata tttaacatta cttatgcgtc
    29701 atttattgct aaaattattg tattaaaata tacatagaat tgatgggata tcatgaaatc
    29761 aaaaatttat atcttgctat tatttctcat ttttttatca gcatgcgcta atacgcgtca
    29821 ctctgaatca gataaaaatg tattaacagt ttattctccg tatcaatcaa acttgattcg
    29881 tccaatttta aatgaattcg aaaaacaaga gcatgtcaaa attgaaatta aacacggatc
    29941 tactcaagta ctgctttcaa acttgcataa cgaagatttt tcggagcgtg gtgatgtctt
    30001 tatgggtggt gtgttatcag aaacaattga tcatccagaa gattttgttc cttatcaaga
    30061 tacatctgta acacagcaat tagaggatta tcgctcgaac aataaatatg ttactagttt
    30121 tctattaatg ccaacagtta tagtagttaa ttcagattta caaggagata ttaagattcg
    30181 aggttatcaa gatttattac aacctatact taaaggtaaa attgcgtact caaatccaaa
    30241 tacaacaacg acaggctatc aacatatgcg cgctatttat agcatgcatc atcgagtaag
    30301 tgatgtgcat caattccaag accacgcgat gcaactgtca aagacgtcta aagtcattga
    30361 agatgttgct aaaggtaaat attacgcagg tctaagctac gaacaagatg cacgcacatg
    30421 gaaaaacaaa ggttatcctg tatcaatcgt ttatccaatt aaaggaacaa tgttaaatgt
    30481 tgatggtatt gctttagtta aaaatgcaca ccctcatcct aagcgtaaaa agttagtaca
    30541 atatttaaca agccgctcag tacaacaacg attagttgca gagttcgatg ccaagtcaat
    30601 tcgaaaggat gttagtgaac aaagcgatca gtctatcgaa aatcttaaga acataccttt
    30661 aataccgaaa tctaaattac cggacattcc acatcataaa tttttggaga tgattcaatg
    30721 acggcataca aaccttatag acatcaattg agacgatcgt tatttgcgtc tactatattt
    30781 ccagtatttt tagtcattat tattggacta gtaagcttct atgccatcta tatttggatt
    30841 gagcatcgta cgattcatca acatgttgat gaaagtcagt catctttaca tcacactgaa
    30901 aagcagattc agacatttat tacacagcat aacaattcgt ttcaagagct cgatttaaca
    30961 aatcatcatg atgttaccgc tacaaaacgt gaattattaa aattaataca ccaacagccc
    31021 gcaacacttt attatgaatt atctggacca aatcagttta taaccaataa ttatgaacat
    31081 ttaaacacta aaaatatgta tttattttca acgcatcagc ttaaattcaa aaatagtaca
    31141 tatatgctta aaatttatat ggctaacacc ccacgtttaa gtgaaattaa gaaagatagc
    31201 cgacaattcg cacttatcgt tgatcaatat gacaatattt tatacgccaa cgatgaccgt
    31261 tttactgtag gtgaaaagta tagaccgcaa caattcggat ttatgaatga atctgtaaaa
    31321 ttaaaccatg ctaaccatcg tctcattatt tataaagata tacatgaaaa cattgaagat
    31381 ggtatcacgt tgttaattgt tatggccata gttcttgttt tactagtaat atttggtttc
    31441 attagcgcag ataatatggc taaacgacaa acaaaagata tcgaaacgat tattcaaaaa
    31501 atttactatg ccaaaaatcg tcatctaggt acatatacgc ctttaaaaaa caacagtgaa
    31561 ctagaagaaa ttaataatta tatttacgat ttatttgaat caaatgagca actgatccat
    31621 tctatcgagc atacagaacg tcgcttgcgt gatattcagt taaaggaaat agaacgccaa
    31681 ttccaacctc atttcctatt caatacgatg caaacaatac aatatttaat tacgttatct
    31741 ccgaaattag cacaaactgt ggtccaacaa ttatcgcaaa tgttacgtta ctcattacgt
    31801 acaaactcac atacagtgga attaaacgaa gaattgaact acattgaaca gtatgttgcg
    31861 atacaaaaca tccgttttga tgacatgatt aagctccata tcgaaagttc tgaagaagcg
    31921 cgtcaccaga caattggtaa aatgatgctt cagccactca tagaaaatgc tattaaacat
    31981 ggtagagata ctgagtcatt ggacattact atacgcctga cattggcacg tcagaattta
    32041 catgttttag tttgcgacaa tggtattggg atgtcgtcat ctcgcctgca atatgtacgc
    32101 caatctctta ataatgatgt atttgatact aagcatttgg gattaaatca tttacacaat
    32161 aaagcaatga ttcaatatgg ttcacatgca cgactccata ttttttcaaa gcgtaatcaa
    32221 ggtacgttaa tttgttataa aataccactt tcaaggggga atgtagatgt ttaaggtagt
    32281 tatttgtgat gatgagcgaa ttattcgaga aggtttaaag caaatcattc cttggggaga
    32341 ctatcatttc aatacaatat acactgctaa agatggtgtt gaagcattat cactaattca
    32401 acaacatcag ccagaattag taattactga tattcgtatg ccacgtaaaa atggcgtcga
    32461 tttactcaat gatattgcgc atctagattg taatgtcatc atattatcga gttacgatga
    32521 ctttgaatat atgaaagccg gcattcaaca tcatgttctt gattatttat taaaaccagt
    32581 ggaccatgca caattagaag ttattttagg aagacttgtc cgtaccttat tagagcaaca
    32641 atcacagaac ggccgtagct tagcatcttg tcatgatgcc ttccaaccat tattaaaagt
    32701 agaatacgat gattattatg taaatcagat tgttgatcaa attaagcaat cttatcaaac
    32761 gaaagtgact gtttcagatt taattcaaca tatcgatgtt agtgaatcgt atgcgatgcg
    32821 aacatttaaa gatcatgttg gtattacaat tgtcgattat ttaaatcgct atcgtatttt
    32881 acaatctctg caattgttag atcgacatta taagcactat gaaattgcag acaaagtagg
    32941 cttttcagag tataaaatgt ttagctatca ttttaaaaaa tatttacaaa tgtcgccaag
    33001 tgattattgt aagcaagcaa aatagattaa caaataacta atgattatgg cgcctccatt
    33061 ttgttctatg aaaaagcgga ctggtagaga agttttcact tttctttcag tccgcttttt
    33121 agagagttta aatataaaac aatactccct acaatattga caccgctata tgtctttatt
    33181 gtaaggagta aattcattga gtcaccctaa gtcgggcaaa taaggatact tcataacagg
    33241 gaattatgat ctgccccgag acatagagat gatatatttg caagggtaga agcagtatct
    33301 ttcatctctt cacgcacatg ccatcggggc aggattgcca actatactgc aataaattgg
    33361 cgatactttt aatccgattg atattagatt tttaaacttc taattttctt ttcttcatag
    33421 aaagcaacga ttcctaatag aatcatgcct aggaataatg caacatagaa gacgatgaaa
    33481 acatctgtcc atccacttaa tgtatatcca aagatgttta aaccgttacg tgttggatca
    33541 gcaatagccg ccaaaccaac ttttgccatt gagtcaccga atagatacgc gaatgaacct
    33601 gtcattccgt ttgctacact gatggcattt ttaggaacaa aaccagtcaa tgatacacca
    33661 attaataatt gcggaccaaa gattaacgca cctaatgcaa acaatgaaat gttaaccatc
    33721 attacacttg tagcatttgt gtagaataag acaacaaatg taatcataaa catacagcca
    33781 atagctacaa ttgcacgacg accttttaat aagtctgata cgtagcccca taataaactt
    33841 gcaactaaag caccaatttc aaagtagaat atcgtattaa ctgcatcgcc tttactaaag
    33901 tgtaaatgct ctgacacata taacggtgcc cagttatcaa taccgattcg tacaatgtat
    33961 acaaagacgt ttgaaacaca tagaatccat ataacaggat ttcccaggat atatttttta
    34021 aagatctccc atttcgtcat accttgagaa tcaatatttt ctttatcgac cggctcttcc
    34081 caaatttctt cagcacgatt ccatcctaat tcttctggat catcttttcc gataaataat
    34141 gttgcgatac caataagtaa tgcaatcacc gatgggaaaa tgaacatccc tataacattt
    34201 ccatggaaga atacattagc accccaaagt gcgacaccac ctgcaatggc accaccgata
    34261 ttatgtgatg tattccaaaa tcctaagtat cggccacgtt tcgttcttgg cgcccatctt
    34321 gaaatcgttg aataacttgc aggtccacca actgattgga acaccccgtt aagtccccaa
    34381 agtacaatta ataatcccat tacagaacca aaataactta aaacaaatcc cataattaaa
    34441 actgtaatcg cagataagat aagtaagaac gagataatac gttttgtgtt acgtccatcg
    34501 acaaaatatc caagtaatgt tttccctaaa ccgtacgtga tactaaatgc taatccgata
    34561 taaccaagtt ctaatgtaga taatccgatt tcctctttta aaaacggttg tgccgcctta
    34621 aagttgtttc gaattaaata catagccata taaacaaaga acactacgaa gaaagcttgc
    34681 atgaagtttc taagccataa tttacgttgt accgataatg gaatgccttt gttcggaatc
    34741 ttatggatat caaaaaagtt catctctgtc acctcaatca ttttcgttaa gccaaatata
    34801 tcacgtcggt gtaagcgctt aatatcctta aaattattaa attatccaaa atactgaaca
    34861 taaacacata cttagaaaca tatttatgta ctaaaattaa taaatagtta atagtatttg
    34921 acaagtttta tctataaaat actttatgtt gtcaaaatca acgcttatac atatgacata
    34981 atgacggagg acatcatgat tctatctata ttacttatct ttttctgtat cagacttgtc
    35041 agcttaaaga tatctattaa tcactcaaaa caattaaaag cagacggtgc agttgaatat
    35101 ggcgttaaaa attcgaagtt tttagcaata acacacgttt taatttatgt attggctggt
    35161 gtagaggcat ttattaataa agatacattt agctttgcaa atggtattgg cttagttata
    35221 ttaatctttg cttatatcat gttatttatg gttattaaaa ctttaggtgg tatttggaca
    35281 ttaaaattat tcattttacc taaccatcct attattaaat ctggattata taaaattaca
    35341 aaacacccaa attacttctt aaacatcatt cctgaattaa tcggtgtatt attattaaca
    35401 cacgcaacat atacaactat tttattagta ccgtatgcgt atttcttata tgtacgtatt
    35461 aaacaagaag agaaattaat gaatatttaa aaatgaaagc ctgtcatcac attagtcgcc
    35521 atcgacgtcc atctttaaag tgcgctctga aatattatat tcagaaacct ttaaagtatg
    35581 tcgtgtctat gaccattgtg acaacaggct ttttttatta tcatatacct cactattgaa
    35641 aaggttatat gctcttttca atggtgagga ttgttactgt gaatgggtat gaccatcgct
    35701 atatacttat ataaagtaca ccttctttac aaacaatgac ttgatttaag gtgtgaaatt
    35761 tttggcaatg gcttggcgac atatgcattt attcaacact gccaccagtc ttacttcaga
    35821 caaacttcag actaacctcg agttcgtatt atgcccacca catgtctgct ggcttgtcgt
    35881 aaatattaac agatttcaaa gtttgacaag ctttttggaa accttcttct actgacataa
    35941 taggatcttc atgttcaata cttaatacat aatcatatcc attaataata agttgactta
    36001 tgatatctgc ccatacatac ggactatgtc cataaccaac tgtacggaat gtccatgctc
    36061 ttgtcgcaac gttaccatat ggttgcatat cagttagacc atacatattt acgttttctt
    36121 gattaatata cgtatcttta gcatggaagt gatgaattgc atttgcttgg cctaatatgc
    36181 gaatcgcagc aattgggtca ataccttgcc accataaatg actaggatct aagttagcac
    36241 cgatatattc atttgtagcc tcacgcaact tcaacatcgt atatggtgta tgaactaaaa
    36301 atcctgcatg caactctatg gcaattttta catcttgctc ttttgcaaac tcagctaaat
    36361 cttgccaata tggtatcaac ttttcattcc actgataatc ataaatttca gagtaggctg
    36421 ttggccaagg tgtaacaggc caattaggct ttttagcggt atcatctgat cctgcaatgc
    36481 cagaaaatgt attaacaact ggcacgtcta atagatttgc taaacggatt gttttacgta
    36541 acgtttcatc cgcttctttc gcttctgtcg gatctggaga aattggattg ttatgacaac
    36601 tgaaaccact tatttgtaag cctctgtctg tgattgactt cataaatgct tggcgcttgt
    36661 cttcattttc taacaactca tctaacttac aaaatttatc tcctgggtta ccacctgttc
    36721 caacttcaat catatccaat ccagattctg cgacataatc taacatatct tcaaaatttt
    36781 tatcgtaaaa taatactgaa aatacaccta gtttcattga aaaacactcc ttctttttat
    36841 attgagaatt ttgtattata tataataatc attaaagttg tatgctcttg ttatcaagat
    36901 cgctacgata aatcgcttct ataagggcat ttacattgcg tgcttcttcc ggtttcacca
    36961 caatctcttc tatccctaaa cacgcattga caaagttacg cgcctgtctc tcaccagcaa
    37021 tgtcttcgtt atgctcaaca ttagctttac tttcaaaaat agctccaaag cggggctcat
    37081 atatttcaaa tggaaataaa ttgataccgc catcttcgcc tgataaacta acgtgtacct
    37141 tatcttcttt gatgtttgca gaccacgaac attcaaactg catgctagct cgattggcaa
    37201 atgtcatata actagtaaca tgatcatcga catcaaattt agtatgatca aatgttcccc
    37261 aatcattaat ttgattcggt tgtttgctca attgattata tgtttttcct agcacttcat
    37321 gcggcaccat atctttacct agtagccaca aagataagtc taacaagtgg caaccataat
    37381 cgattaaact accgccacct tgcaatgctt tattggtaaa aacaccccac ccaggcactt
    37441 tacgcctacg catcgcttgt acacgtgcta ctaatggttt accaaccaca cctgcttcaa
    37501 ttgctttttt agcagtaatt gccacatctg tgtgacgata atgataagcg acagttaata
    37561 atttgtgatt tttattagcc gcttcaatca tgcgatcaca ctcttccgtc gtcattgcca
    37621 ttggcttttc acacaataca tggacgccat gattcaatgc ttctatagaa agatcagcat
    37681 ggaatttatt aggtgtacaa atgaccaccg catcaacaag tttaaacagc tcgctaggtg
    37741 tctcaactgc atgaggtata ttaaagcgct tcgcaacatc aatcatctgc actgtattaa
    37801 tatcttgtac tgcaactaat gagactgtgt ctttgagttt cagcaatgct ggaatatgac
    37861 ggtcttgtgc aataccacca acacctatca caccaacttt taattttgtc atgatgtgcc
    37921 tccttaccgt atgatgttat tcaaagtaaa ttgctttgcc tgattttgca gactgataaa
    37981 tcgcttcaag aatttttgta actaccattg cttgttccgg tttcacaact ggttcagtat
    38041 catttacaac tgcatcaatc caagcttttg cttcttcttc agcttcatcc acttcattac
    38101 cttcataaaa gtcgacgcct ttgttttcca attcaacgtg tttggtataa agtgtgccca
    38161 tatcttcacc atgaatacgt agaccatctt tcatatcagc acctgctttc gttcctgata
    38221 atgaacattt tgcctcatcc acttctaaag aattaatcgc ccaagcggat tctaaaatga
    38281 tcgtcgctcc attcttcatt ttaataaagc caaacgcaga atcttcaact gtaaattcat
    38341 ctggattcca tgaaccccaa gcgtttgcag catcatgctg tttatttaat ttatggaatg
    38401 ttgaacccat cactgattct ggttcataat tatccatcat ccataacgtt aaatctaaag
    38461 cgtgtgtacc gatatcgatt aatggtcctc caccttgggc ttcttcgttt agaaagacac
    38521 cccatgtcgg tactgcacga cgacgaatag catgtgcctt tccgaaataa atgtcaccta
    38581 agtcgccacg ttgtgctgat ttatataaaa attgactatc tggtctgaag cgattttgat
    38641 aacctattgt taatttttta cctgttgatt tagctgtatc tatcattttt tgagcttctg
    38701 ctgtcgtttt agccattggt ttttcacaca tcacatgttt accggcatgc aaccctgcta
    38761 cagtaatttc acaatgcgag tcatttggcg tacaaacatg gataacatca atcgtgtcat
    38821 cttttaacaa tgctttgtaa tcatcataaa cctttgcatt gtcagtaccg tatgcttctg
    38881 ccgcacacgc tgctttcgaa atgtctacgt cacaaaatgc gatcatttca acattttcaa
    38941 ctttttgtaa acttggcatg tgcttgccat tcgcaatacc accacaccca atgattccaa
    39001 ctttaatcgt catcataacg tcctccttta aatctttatc gtcattagtg gttctttatc
    39061 atttctgccc tactcacatt ttcaaactaa ccttttgtcg cacctgttgt taaacctgat
    39121 actaagtagc gttgcaagaa caagaatacg attgctatag gtactgcaat cataattgcc
    39181 cctgctgcaa acactgtgaa attatttgca tacttatcat taataaagtt gaacaatcca
    39241 actgctaatg tgaatttttc aggacttctt aatagtattt taggtaatat aaagtccata
    39301 aatggcccca taaaattgaa caaagcaaca actgctaaaa tcggcttagc taatggaagc
    39361 ataatttgca agaaaatacg catatggcca gcaccatcaa ttttggcaga ttcatcaagt
    39421 tcttttggaa tcgtatcgaa gtaacctttc actaaaaagg cattcatcgg tattgaccca
    39481 ccaatatata caagtgttag tccaaataaa gaatctaata atccaattgt atttagcaaa
    39541 atatagattg cgaccattgc cattaataca gggaacattt gtaaaatcaa aaatgtaatc
    39601 agcccgtatt tacgaccaac aaagcgatat ctagaaaaag catatgctgt taacgtgaca
    39661 aatatcacac taaacagtgc atttgcagat gctacgataa gcgtattttt ataccaagtc
    39721 aggtattgac tactgtcatc gaatagtaag aatgcataat ttttaaatgt tgcattgtct
    39781 ggtatcattt tggcaccata caagttcgta cctggattaa gggaaatgcc aaatgtccac
    39841 agtagtggat ataaaatgat gacaaacatc atcgctataa aactgtaaat accgattgct
    39901 tttaatatgt ttttcttctt tgtcatctta taaacctccc tcatctttaa acgtacttgt
    39961 acgtctgaat tgaataaatg cgacaatagc aacaataaat ccaataatta atgacacaac
    40021 tgcacccatg ttgaagttgt taaactcaaa tgtcagatta tacacccaag atatcaagat
    40081 atctgtacta ccagcattct gccctgacac tggtggacca cctttattaa atagataaat
    40141 aagattaaaa ttattgaaat tacctgcata ttgcataatt aacaatggtg ctgtggcgta
    40201 aatgacatgc gggaatgtga tgtttctaaa cttttgccaa ctagacgcac catccatatc
    40261 tgctgcttcg taccaatcag atgaaatact ttgcagtact ccagtgaaca gtgcaaagac
    40321 aaatgggaaa ccaagccata cttgaatgcc gattaatgcc acttttgccc aaaacggatc
    40381 acttaaccat gctggtgcta cacctaataa aggttgcaaa atatcattat ttatcgcacc
    40441 aaattcatca ttaaataacg ctacaaatat taaaattgtc acaaatgatg gtacagccca
    40501 aggtaggatt aacacagtac ggataaattt cttacctttg acgacagggt gatttacaat
    40561 aattgccaaa aacagcccta atgcaatttg aagtgtcgtt gcaacaagcg tccatactaa
    40621 tgtccaagta ataacactga aaaatgtttt acgccaaacg ccaattgtga ataacgtttt
    40681 aaagttatct aaaccaaccc attctaatgt gtgtctcgga ggcgcgttgt ataaattgta
    40741 atttgtaaat gctactccaa acataaatat taatggaaat acaactacaa atatcaataa
    40801 gaatgtacct ggtgagatca gtaagtatgg gaacgtcttg tcccatgttg caaccatacg
    40861 tgccttcgga tcctttattt cctcattgcg attaaatcgt tcagcattac gatatgcatc
    40921 taaaatatta atgacatata gcatgattgc gaaagcaacg agtaagatag aaataatacc
    40981 ttgtgcaagt aagacacgag aatcgtctaa cttaggtact gtccctaatg tgaacaatcc
    41041 ccaaaaacca atatttaaaa agctataaaa aacagaaata aaactgatga aaaagataaa
    41101 aaatatcgtc cctttaatgg gtcttttatt ataaaactgt cccaaaccag gtataacaga
    41161 taataatgcc gctaatttag ggttacgttt cgtcataact aatctccttt cttatcattt
    41221 tgtgacggat gaagaatctt aatattttgt gttatatcat tcgtcgcctc atctaacgct
    41281 tgtttaggat tcttaccatt tgagataaat atgcttgcat tgcccatcgg ttcccaaact
    41341 tgtcgcattt caggaatatt aggcatcggt tcagcatgac gtgcttgctt ttcaaacact
    41401 tttaaatttg gattagatga tttcacgtca acacgtccag taatttcgct catttcatct
    41461 gtatattttt gtaatgtatc tttactagtg atatacagca ttaaatcttt agcccaatac
    41521 ttatgtttac tatattcaga taaataccaa ccacgtacac cgagaaatgg tttcataggt
    41581 ttgccaccat ctgtaggcaa tgttgttact cctaaatctt taccaaacgt ttcttgatat
    41641 tcattaatgt tccatggtcc agtgacaaat tgtcctactt ttccttcttt aaaaagacca
    41701 atcatgacat catgtgttgc tgccttagga agataccctt tgtcgtacca tttttgtaat
    41761 cgttcagcat tcttgacgac atgttttgaa tttagtccta gctgatgaat atcatattca
    41821 ctgccatttt tcttgaaaat ataatcatca ttgccgaata aaaacggata attaaaatag
    41881 aaatttttag catcaaataa cataccgtat tgtttctttt tactatcagt tagcttggca
    41941 gcattagctt caacttcttc taacgtttgc ggtgcatttt tcactaattt tttattataa
    42001 aaaagtgcgg ttgtttcaac gatagctggc aatgctagtt gcttattgtc ataattcatc
    42061 gctttaagtg cttgcttatt gaaacctttc aactcatctt ttgatagttt gatttcagaa
    42121 gctaagcctt gtagataggc acttccagta ttatcatgtg ctaagaaaaa gatatctgga
    42181 ccttttccag caggagcgtc tagcgaaata ttttctagtt gatcattttg accaatattt
    42241 acaagcttta cttttatgcc agttttttta gtatattgat ccgtaatttt tttataaaac
    42301 gccatttgct tgtcgccatc cacccacatc gtaagttggt taggcttgtc tttagaatta
    42361 tttttattca atgctttatc aatatcttct ttcgaacgat tagggccaca tgcagttacg
    42421 attaataaca ttaccacgac taacgtgata cattttaaaa ttttagacat gaatactccc
    42481 ccttagacga tacgatttcc tgttttttca tcaaaaaagt gacacttatt catatcaaat
    42541 gctagtgtaa tcttgtcgct cgccatcact tgtgttcttg aatctaattt agaaatcaat
    42601 tccattcctt ggaatgtgct atgtaccata atttctgaac ctaacagttc ggatacaact
    42661 acttcagatt caaattgtgt ctctggagaa gtttgaataa atattggttc ttcatgaatg
    42721 tcttcagctc gaatacctaa aataatttcc ttgtctaagt agccagcagc ttttaacttt
    42781 tcaaattttt tattatgtaa tttaaaatga tgtgttccta ccttcaatcc gtccatttca
    42841 actgtagcat tcaacatatt cattgctggt gagccgataa attgcgccac aaatatgcaa
    42901 ttaggggcat catatatttc tctaggtgtg ccgacttgca taatgtcgcc atctttcaaa
    42961 acaacaattc gactagccat cgtcaatgct tcagtttgat catgtgtaac ataaattgtc
    43021 gtagtattaa gtcgcttatg taatttcaat atttctgtgc gcatttgtac tcgaagcttc
    43081 gcatcaagat tcgataatgg ttcatccatt aaaaagactt tcgcatccct aacaatagct
    43141 ctgcccaaag caacacgttg acgctgtcca ccagataacg cttttggttt acgaccaaga
    43201 tactcagtta accctaatat ttcagctgct tcattaactt tttgttcaat ctctttttta
    43261 tttactttac gtagctttag cccaaatgcc atattttcaa aaacagtcat atgtggatat
    43321 aatgcatagt tttgaaatac catcgcaata tctctattct ttggttgaac atcgttcatg
    43381 cgttccccat caatataaaa atctccagat gtgatagact ctagtccagc aaccattcgt
    43441 aatgttgttg atttaccaca tcccgatggt ccaacaaata caatgaattc tttgtcagta
    43501 atatgtagat taaaatcttt cactacagta ttgttgttat catacgtctt tttaatatgc
    43561 tctaacttta gttctgccat gttatgcctc ccatactttg tttacatttt gattgtgcaa
    43621 tacactttgt gaaaacgttt gcacaaatac ttacataaat catatagtca ctcattatta
    43681 atgtcaatca taaattgtaa acgtttacat tactgtcaga attaaacttt tatacaggga
    43741 attgatatag tacacaacta tttttagtgt gacaataatg attttttacg tgcgaaattt
    43801 aactgccaat gctagcagcc ttgtcttaca ttttaagtaa tatctaaatc aaacaagtat
    43861 gccttagcat ttcgattttc attgaaaaaa gcaaccatta tgtaagtatt atactcacac
    43921 aatgattgct taatttttta gactgcgggc actcttctat aactacaaat catataggct
    43981 acgttcccca tcaggtaact gatcgataaa cggttttgga tcttctgcat atgcattacc
    44041 tacgccaccc ttcattcttt gaaaatgtaa atgtgcgcct gtcgtttgtt tacctgtatt
    44101 gcctgaatat gcaataatat cacctgcttt gactcgatca cctactttga cattatattt
    44161 gtctaagtgt agataccact ggtgatattc tccattgtct tcggcaatct gtaatacctt
    44221 gccgcctaat tcattattaa agatacgtgt tactttacca ttcgtcggcg ctttaattgg
    44281 tgtacctttt ggcaatgcaa aatcaatgcc ataatgctta ccatcaaatg gtgaatgttg
    44341 atatttacca aaagtctcag taatccgact ccctctaaaa aagtcttccc attttgagtg
    44401 tccttttcct ttatgatttt gtgtcaattt atcggtatat tggttaaacc atttttgaat
    44461 gtcatctata taaataatcg taaataatag tgaaagaaaa atgataattg ttgccaaaat
    44521 acgttttggt cgctttgtca tagtggcatc catccttcat atattttggg tattaagtat
    44581 atattctaaa tcaactattt cataattaaa tgccctcata ctttagtagt actgccaaat
    44641 atagatgaag aaaatgatgg taatttgaat attttgattg atattaaatt ttatatcgag
    44701 tataataatt atacttaagc aaataacaga aacagaggag aattttcaaa tggcaaaagt
    44761 attatatatt acagcacacc cttttaatga actggtatca aattctatgg cggctggtaa
    44821 agcatttatc gaaacatatc aacaacaaca tcctgaagat gaagtgaaac atattgattt
    44881 atttgaaact tatattccag ttattgacaa agatgtatta actggttggg gaaaaatgag
    44941 taatggcgaa acgttaactg atgatgaaca aatgaaagtt tcaagattga gtgacatttt
    45001 agaagaattt ttaagtgcag ataaatatgt atttgtaacg ccgatgtgga acctttcttt
    45061 cccacctgta gttaaagcat atattgatgc aatttcaatt gcaggtaaaa cttttaaata
    45121 ttctgctgaa ggaccacaag gtttgttaac tgacaaaaaa gtattacaca ttcaatcacg
    45181 tggtggatat tatactgaag gccccgctgc taatttcgaa atgggcgacc gttatttaag
    45241 aacaattatg acattcttag gtgtgccatc atatgaaaca atcattattg aaggacacaa
    45301 tgccgaaccg cataaaactg aagaaattaa ggcaactagt attaataatg ctgaaaaatt
    45361 ggcaacaaca ttttaattgt tatctacact gtgtgactaa tcaaacgtca tacagtgttt
    45421 tttattgtat acctttcccg ttttttataa atatctatta ttttcataaa tttaaaattt
    45481 gatattcact ttaatactga tgtaaatatt tttaaaatat attattatag tgttaatgaa
    45541 gtattttaag aaacggtggg aatgtatgaa agcgtataaa attttctggt taaacttagc
    45601 agcaattatt attatttcaa ttgtagtcag tggcggtatg tttttggcaa tgaagtggga
    45661 acaaattcat ttaaaagatg gtcttaaaaa agtactaagt acatatccta ttaaaaactt
    45721 agaaacgctt tatgaaattg atggtcacga taatccccat tacgaaaata atggtcaaga
    45781 caaatggtat atagaatcct cttattcagt tgttaaatca gatgagcttt taaaagaaga
    45841 ccgtatgttg ttagaggtag ataagaacac acataaaata actggtgatt atgacacaac
    45901 aataaacgat agaaaaaatg ttactcactc gacacacaaa agttatccaa taaaagtagt
    45961 taataataaa atcgtctgca caaaagacgt aaaagatccc gaactgaaac aaaaaatcgg
    46021 aaactatcaa tttttgattc aaagtggcga tttaccaagt attttaaaca gtaatgattt
    46081 gaaaatcaca catgatccta ctactgatta ttataattta tctggtaagt tgtcgaatga
    46141 taatccaagc gttaaacaat taaaacgtag atataatatt cccaaaaatg catcaacaaa
    46201 ggtggaatta aagggaatga gtgatttaaa aggtaataat catcaagatc agaaacttta
    46261 tttttatttt tcaagtcctg ggaaagatca aattatatac caagaaagtc ttacttataa
    46321 taaaataagt gaacattaat acttatgatg taattataga aataaccaaa tcattgatta
    46381 aaatcctgca ttataaaaac acctcacatc actcgatcaa ctgtaccaaa ccacatcacg
    46441 ttagatttta gactaactat tgtgatgtac atcaaaaacg aatttgtgag gtgttgtata
    46501 ttttacaaag gtgactagcg tttcgtatag catttccaac attactacac tcaagcgtca
    46561 ctctaaagtt cgaaatcgaa tcctttcatt caataaaagc ccttatccac tacaaacttc
    46621 atatcaagcg tataaactat cttgtgatac tatctcgatc atatctatag tatgcatttg
    46681 tgttccgttt cactgaatga tatgcttcat catttaaata taaaccgtta tctttcaatg
    46741 ccacttgata agcattcttc cgtgccaacc acacaatatc tatataattt tctccagcga
    46801 tttcataact tctcaaatct tcaatatgca cactaacttc agggaaaatg attctaataa
    46861 cactctcctc aatccaaaat ttgccatgca tccatcgcac ttgttcagca tataatggca
    46921 actgcacatc attgaaatat acacgaaagc cgtcattatc atacatttgc cgatatggta
    46981 atggctgttt tctaatcact aacacctcgc cacccattac ggtgccttct ctagtatcat
    47041 cactgccacc cgaagcttca tacgttgttg ggtcaacttg tagtccatgt acatctccaa
    47101 tataggcatc tggtttatgt tccattgcat gtccacgtgc aatcaatgct aatattgtag
    47161 attgtgaaaa ttgaggctcc cattcaatgc gattaggatg gctactataa attctaggtt
    47221 catctatagc ctgctgaata tccataccaa acactaatac attgattaat gtttgagcaa
    47281 cactagcaat gatacttata gcaccaggtg cacctactgt taatattggc ttcccgtgat
    47341 gcatcacaat cgttggagcc atgttactta gtggtcgttt ataaggtgca atttcattga
    47401 tcccacccgc cacaacatca aaaccatcca ttgtggtatt caataacacg ccgtagcctg
    47461 gaatcgtgat acccgatccg taaatcatac cgattgatgt cgtaaatgaa gcaatattac
    47521 cttccttatc aatcacacaa aaatgagttg tttcggtatg attttcttca acatctgtat
    47581 gactaatgca attagacaca acaccatgct ctatatcaat atttgcttta ttgctatcaa
    47641 tgagcttact gcgtgctttt aaataatcgt catcaattaa tgactgtaca ggcacctcat
    47701 gaaaattatc atccgccaag tattgcgcgc gatcactata tgctaaatgc attgcctgta
    47761 tcaaatgatg caagtaatcg acagatcttg gacccataga tggtaaatcg acatgttcta
    47821 ataacttcaa tatttgaatt accgtgatac cgccagaact agatggtccc attgaataaa
    47881 tgtcatagtc tttaaatgtt gcactgattg gcgctttaat ctgaatgtca taattggcta
    47941 gatcctctaa agtgattgtc ccaccacatg ctttaacaac attaactaat tgtttcgcaa
    48001 tgtcaccttt ataaaatgca ttaaaccctt gttctcttaa tatttgaaat gtcttgccta
    48061 attcgggttg tacaatccaa tcaccttcac gccaatattg attttcatgc gtaaatactt
    48121 gcgccgtttc gtgataattt gtcaatcgtg cgtgttgctg gcgcgaatat ttttcagtag
    48181 cccaattggc tgaatgacct tcaatggcta gttcaattgc aggattaatt aaatcttcca
    48241 atgacaattt agcataacgc ttgtgaatat aatcaaacag ctttggaatt gctggcacag
    48301 cgacagtttt accatgtgta gtcatatcaa aaaatgattt atattcgcct gaatcatcta
    48361 gataaaattg tttgtctaca tgtgcagggg ctgtctcacg cgcatcaaac gcagttatac
    48421 tgccagtact ttgctcataa tatagcaagt acccgccacc accaatacct gatgcaaatg
    48481 gttctaccac attcaatgcc agttgaattg caatcactgc atccatggcg ttgccacctt
    48541 gatctaatac atccttacca attttagccg caagaggatg tgatacggaa attaaccctt
    48601 ctttagatgt ttttgtctgt ttgtcattta agttaatgac catactatat cctcctactt
    48661 tctgttaaat atttaaaaca ttattgatta atggcttttt ctactttttc taaatcttga
    48721 cgttgctcgt taccagtatc gacaagtggt gtaatcggtg atgcaatttt aaatttatcg
    48781 ccacgataaa acttaataaa ttgatcctga tctatcgcat taactactgc ttgtctcaag
    48841 tttggatgcg tcttaaatat acctttttta atatttagca ttaaaaagac tgacttgcgt
    48901 ccatttttgc gaataatgct taaattttta tccgacttaa ttaaatcaaa atgtttttga
    48961 ttcacatctg ccaacatatc aattgaatga tttctaagtt ctgacaatgc attattcgga
    49021 tcaccattaa acttcaatgt aatattttta attttagctg gtccataact acctttttct
    49081 gtttcgttga atcctggatt acgttgaaac gttgcttgat atgcattttt ctgtgtcata
    49141 atgtatgcgc cacttgcata aagcgcattt ttcccatctg aatttgcagg aattgtactg
    49201 ctatccccat atccttttgg atattcttga tttacttgat taacaaattt tttagataaa
    49261 atgcctgccg aagagtgtgt taagtaattt acctctcgag gcatcgattg atctgtcgta
    49321 attttaacaa tttgatatat accgccttta ttatttactt tttgaccatc tgtcgttaac
    49381 gatttgacgt tatacgcttt aatcaactta tcatagatgg aattatcgtc cttgtctttc
    49441 tctttacgca actgatcgat gtcctcatct tttaatatct tgatgtcatt tatatgcttg
    49501 tgcatattgt aagtattatt gttaggcaca gactttttat cacgtgctct atctaaagaa
    49561 aacttaacat cttcagccga tacacgctct ccagtattac gtgcttgtcc attgaccact
    49621 ttcgcaaaat aatcatcatc tcttaacaag aaataaaacg ctttattgtc cttattcaca
    49681 gcataatcac gacttaatga acctttcgtt gttaagtggt cattttcatc taataataat
    49741 aaccttgtgt acatattcat attaattgaa tatactgacg gcgcaattga acgtattgga
    49801 tccaatgtag gaatttcacc atcttgttgt gtcatcacaa gtggccgcgt atctcgttct
    49861 ctactattgt tgtaatcaaa ttgttgccat attaatgcac gtgaatttgg caatccaaca
    49921 ctatttttat ccaacacttt attgtcatat actaaattct tttttgatcc atataatggt
    49981 gccatatatc ctttatcaaa gacaacttca tcttcaattt gtttatatgt tgattttaca
    50041 tctgcttcat tttgagtaga agctttattt aacaactggt caacatgctt atcttttaat
    50101 aaactatttg accctgtaga actaaacagt gctgtcatag catagttcgg gtcaccgaat
    50161 actgtcatcc agtcatcgat ttggatatca taattgccgg cttgacgttg tgtacgatag
    50221 cttccataat ctggttggat attcatcttc acgttaaatc ctgcattttc caattgatct
    50281 ttgacgatat tcatatcatt ttcataactt gcttgtccta ggaaatgtat cgttggtcgc
    50341 tcgcctttca cttcaacttt cgatgacttt tgagccactt ctgatttcgt agggacacca
    50401 caaccactta ataccaacgc taaaactata attgcgatac taatgatttt cttcacatct
    50461 atccctacct tcttaatgaa ttcttggatc tagtgcatca cgcaccgcat cacctataaa
    50521 attaaatgct aaaacgacga acataataca aataccaggt acaatagcta aattactgtg
    50581 tgtttctaaa tagttactac cggtacgcaa aatgttaccc cattccgcta cgtcaggcgc
    50641 aacgccaagt cctaagaaac ttaaactact tgttgttaac acaactacac ctatatttaa
    50701 cgaaaaacgt acaatcattg gcgcaatcgc attcggtaaa atataacgcc atatgatatt
    50761 ccaagtgttt tcaccagtga tacgtgctgc atctacatat tccatgcgtt taatttctaa
    50821 aacactggca cgcattgtcc gtgcaaatga tggtatatta ccgatactta atgcaataat
    50881 taaatttgga atacttgctc caaatgatgc aataattgcc accgctaaca ataatgatgg
    50941 aattgcaaac acgacatcta aaattcgcat tattaaatta tcaatatgat taaaataacc
    51001 tgcgatagtg cctagtaaca caccaaaaat aactgcaata actactgaaa taattgaaat
    51061 tgaaaatgtc agtttcgttc cgacaactac gcgtgtaaat aagtctctac caaaatcatc
    51121 agtaccaaac ggataggcaa gactcggtcc atgtaacagt gcattgaact gatttttagt
    51181 agccaatgtc gtatcaaatg taaattgtga cacaattgat aatgtcagca tgtagactaa
    51241 aataagtaac ccgataatcg caatacgatg tctagtggtt tttcgtataa acgattccca
    51301 cccgttataa ctatgtattt gcgatgtacg ttggtaacgt ctaatactta caaacattaa
    51361 taatgtaaat acgttgcctg ttaatgtcat caacaataac aacacttcga cgatacgtcg
    51421 ccataggtca taatgcttcc atgtttgttc cgttgttaaa atgataatta aaatgatggt
    51481 taaaacgatt agcaatgttt cagcaatata gaacgtatcg gccacataac ctttaaaaag
    51541 atttaatgca ctcgttaata taactaaaat ataagttgca atagcataac ttgcgaatag
    51601 ttttaaggaa gctatctttg ttgtaagttt taccatatgc ctcacttcct ttcgttaatt
    51661 tcactacgta attttggatc gattaaagca taaaatatat caataattaa gtttgctaaa
    51721 gatattacaa ttgatatata tacgacccca cccatgactg ctggaatatc aggtattagt
    51781 tgtttttgga cgatataacg accgatacca ttaatgttaa atacttgttc cgtcactgct
    51841 gaaccgccta gtaactctgc cactagaaga ccaactagcg ttacaattgg aataatcgca
    51901 ttttttaaaa tatgctttat aactacttgt gtcgttgata gtccttttgc ataagcagtt
    51961 aaaacataat cactgcgcat tacttcaagt acagaagacc ttgtcatacg cgtgatagaa
    52021 gcagcaatac ttgttccaat gacaagtaca ggtaaaatca acgatattgg atgttctggc
    52081 atataagatg gtggcaaaat atccaatttc aatgagaacg ctaaaatgaa taataaccct
    52141 tgccagaaac ttggaataga taaaccaatt aatgcaatta tcattaacgt gatatcaagc
    52201 caactatttc gcttcatcgc actgataatt ccaattggta ttgcaataat taatgccaca
    52261 attagcgcta atactgcgac aattattgta attggaattc tttcgccaac tgctttagtc
    52321 acaacctcat tccctttgta agtcgtacct aagtcaaaag taaaaacacc cttgatggta
    52381 tcccacaatt gaataaaata aggctcgtta agatgatgta atacattgaa ttgatgtatc
    52441 tgagcctttg ttgcattttg tcccagtatg ctataagccg catcaagcgg tgaaaaatac
    52501 agaatggtaa acacactgac aataacacca atgatgacaa tcacagccat gacaattcgc
    52561 tcaaaaatat atctaactaa tggctgtaaa taaatagtca ataagatgaa catcggcaat
    52621 gccaatatca ctttgatcat gatgaactta tgaaataata cattttcaaa gtatgttgaa
    52681 aaatgtgctt gttcaatatt ctttgaattc gtattagaac tttgtgcctt gaatattttt
    52741 aatgcttctt tatgtatttg tgtggatgac ttttgctgcg ataaatattt atatttttga
    52801 tgtaacgcct gttcaatttc tgaaatttca gaattattgg cgtaaaaatt ttttatctta
    52861 gcagaaaaga aaaactttat cactgcatat aaaaatattg gcaagcttaa taccgataat
    52921 acaaacttgt aaaatgcctt gtgatacaac gacttaaaag attctaaata agtaattatg
    52981 ataatcactc cttgtaaaat aatcttgagt agattactat gatatacaaa agtatagaat
    53041 aaatttacac atttgtgaat agggaggcac aacatcatgt caaatttatt agaagtcaac
    53101 agtctgaatg tacaattcaa ttatgatgaa gctacagttc aagcggtaaa aaacgtctct
    53161 ttcgaattac gaaaaaaaca tatcctaggt attgttggtg aatcaggatc aggaaaaagt
    53221 attaccgcta aatctatttt agggctacta ccagattatc cagatcacac gttaacagga
    53281 gaaattattt ttaatgggca atcgttaact aatttatcaa cttcagcatt acaacaaatt
    53341 cgaggtaagg atatttcaat gatttttcaa gatccactct cttcgttgaa tccaagatta
    53401 acgattggca aacaaattac agaagtacta tttcaacata aacgtgtatc taaatctgaa
    53461 gcaaagtcta tgacaataga cattttagaa aaagtaggta taaaacatgc aactcgacaa
    53521 tttgatgctt atccacatga actttctggt ggtatgcgtc aacgtgtcat gatagcaatg
    53581 gcattgattt taaagccaca aattttaatt gcagatgaac caacaacggc attagatgcc
    53641 agtacacaaa atcaattact gcagttaatg aagtcccttt atgagtacac agaagcgtct
    53701 attattttta tcactcacga tttaggtgct gtgtatcaat tttgcgatga tgtgattgta
    53761 atgaaagatg gaagtgtcgt tgaaagtggc acggttgaaa gtatttttaa atcgccacaa
    53821 catacctata cgaaacgctt aatagatgcg attcctgata ttcatcaaac gcgcccgcca
    53881 agaccgttaa acaatgatat tttattaaaa ttcgatcgcg tgagcgtgga ttacaagtca
    53941 ccaagtggca gcctataccg agcagttaat gatattaact tggctattag aaaaggcgaa
    54001 acattaggca ttgtcggtga gtcaggatca gggaaatcga cattagctaa gacggtcgtc
    54061 ggtctaaagg aagtgtcaga aggctttatt tggtataacg aattaccatt aagtttattt
    54121 aaagatgatg aattgaaatc tttacgacaa gagatacaaa tgattttcca agatccattc
    54181 gcatctatta acccaagatt taaagtcatt gatgtgatta aacgaccact tatcattcac
    54241 gggaaagtca aagataatga tgacattatt aaaactgtcg tatcgttgtt agaaaaagtt
    54301 ggcctagatc aaagtttctt atatcgctat ccacacgaat tatctggtgg gcaacgccaa
    54361 cgtgtaagta tcgcaagagc acttgctgta gaacctaaag tgattgtttg cgacgaggca
    54421 gtgtcagctt tagacgtatc aattcaaaaa gatatcatcg agttattaaa acaattacag
    54481 ttagacttcg gcatcactta tttattcatc acacatgaca tgggtgttat caatgaaata
    54541 tgtgatcgag ttgcagttat gaaaaatggc gaaatcgttg aactgaataa cacagaagat
    54601 attatcaaac atccgcaatc agactatgca aagcaactta tttcagaagt agcatttatt
    54661 gctagataaa agttatgcgt tgtgcaactt tatcactgta tggtctggaa taaatcgggc
    54721 aacttctgat gtgttatgtt agatgacttt gtcatactaa ctatattgtg ctatgttagg
    54781 gctcggcacc atatcaagtg ttagatgctt tgccatgatt taagggtcac acccatactt
    54841 tgggcatttt aacacatgat aaatcccccg tcactatata aagtgcgggg gattatttgc
    54901 attttattag aatattcaga cttttaagtg tgtcaactta gcttagtcaa tgtatattta
    54961 acgtcactta ctctttttct ttcataatta acacattcaa ataaatcttg atcaaaaaac
    55021 acaaagttaa aagtaccatc ttgcaatatg ctctcataca ttatcccgtc atatttaagg
    55081 cttcgaatat aatcagctaa atattgaaat ggcaaataat ctattccttg ttcatcgctt
    55141 ggatttgtta ttcctttatg aatctttttt aatgtttggt gatttacaaa atactttcta
    55201 aatccatcat cgccagcttt gattgcatta ctagttaaat tagttaaatt cgcaattttc
    55261 aatttctctt ttgtaacgtt tttttgtaac ttaaccttac ctatataaat aatgtcatta
    55321 tgcttaggtt taacttcttc tatactgacc tgttcttttg tactaaggta taatacgctt
    55381 atccatttag aattcaatct tcctgccgtt gcaaatccct ttggtggtga cattagttca
    55441 cttttctctg taatgaactt aactattcta gatctatata atggttcaaa tctttctcta
    55501 aattcctcaa tactatagta attagtagtg atatcgagaa agaacgctaa attctctaaa
    55561 ttgatcatat tattatgaaa tctattttta tacttcaagc tctcacaaaa tccatcccag
    55621 tcattatttg ctacaattag atttttattt gtatattttt tatcgtttat gattttagcg
    55681 cctactaaat cttccaacac tcgtctatct aaattttcat catctttaaa aagttcattt
    55741 aaaatacaac ttatttgagc ttcctcaaca ttaaatatac tccagtcgtc ttttaatgct
    55801 atttcaatct ttttaccttc ttttaggcta aaagtatctg gtaaatttat actaatatca
    55861 tataattcta atgctggtct taaataatct ctaataagtt ctaatttatc tatgtcctta
    55921 gtcgtatcaa atattttaac accaagatga tcgttatcaa tatcacaatt gtcaaatttg
    55981 ctattatcat ttgcaatgat ttctacgatt tcagtattat taaaacattt ttcacatatt
    56041 ttcattttga gactccaagt atctattcat aatttctaga tgatgcatga tagataacct
    56101 tttaattaaa cctaatcctg gatacttatt attttcattt aattcttcaa attgtccaag
    56161 cgcataagat ctatttttaa tatctaagtt ttttgaccat gttactaatt tatctacagc
    56221 ctcaaaaaac tttttacctg gatttgatct atcattatta gaatcagaga caaaatgttt
    56281 aactcttagt tcattagcct catcaaaata gacaatatgt atcgcaatag ctaatggcga
    56341 aaagccaccg tctacatatt ctccacctat aacagaatag tctgaaaatg ctacgtatcc
    56401 atcttcctta taataaagat gatcgtcgct ataaaactcg tcatcattat ctaaataatc
    56461 aacattacga cttagtttat taaaaggatc cataatgatg cctttgtttg gaatattacg
    56521 caatttacgt ttaatgtgtc tatcatctgg tacaaaaacc atttcaagtt tatttttatc
    56581 tccaaacatt tcccagtcat ctacacttgt taaaatagct aaactattac ttgaaaattg
    56641 ctgtttcaac ctatcattgc cttcatcaga aggacccaag taagcttttt gaataacact
    56701 tttgtttttt gttattattt cagttaaatg ttcaacagtt tcgttactaa tctcttcttc
    56761 agttaacttt gaatttacta ctaaatttat agagaatgct ttttcaccac agtattgcaa
    56821 agtattttta aatgtggttg tatatttaat tggttcaatt ataggttgaa tacaatcacc
    56881 aatcaaacct ttctctaaca attctctaat tgcaaatagt tcattttgtt tcccacgcaa
    56941 ataaggataa tacatattaa tcctccttaa gaaaatgata tagtttattt ttatctttct
    57001 ttgtcatttt tgcgaaatat aacaagaact taatttcagt cggagctgct aaaaattcat
    57061 ctttgtattc tttcatgttg cctctttttt tcaactcaga aatcatattg ttatgcagtt
    57121 tcgtcattgg tatttcttta aacacgtcaa ataacacatc atactctgtg aaatcattat
    57181 aagttggcac atccatatga ttttgtgcaa taactctttt tctttcttct tttcgtaaaa
    57241 agttgtatat cgcttttgtg tttaagagac tattgttttc tttaggtttt tgaacttcac
    57301 tcagtgtatt tctagaagtt aacactaaaa ttccaactgt tgtatctttg tatctagcca
    57361 taactttatt cagatgtttg tcatttgtaa tgactacgac ataattaaac acttcataat
    57421 aatcattaat ttgattatct aatctatcca gcttatctaa ttctgtttta atctcataga
    57481 caacgccttt gccgtttaac aatataaaat cagcaatact tttccctatg ggcatctcag
    57541 aaagtgcagt agttgtatta acagaatgtc gtcctagaag gagtttatta agcattgtgt
    57601 ttttgtaaaa atattcgtta cgataagact ttttaagata attatataaa gatttcatct
    57661 tctgtattac attgatattt ttaggtccct gtatataaaa atcatatgcc tcatcaacaa
    57721 catcactttt atttttgatt gcaatttgat gtaatatctt ttgactaaaa acacgattaa
    57781 tcatatttct attatccata gctacgcacc tccttcaaca tacatttaat acttatcctg
    57841 ttaatattat aaactatttt aaatgcctat agacattatt tctatacact tacgatgata
    57901 aaattgtatt gaaaccaaat gatgattgcc aaacgcatgg taactaacaa aagatagaaa
    57961 ataataaatc cccctacact aatctagcga gggggctgaa tcattacaca tcgtattttt
    58021 cagttgtctc tgcaacaaaa gattcaatgg cttttcttgc tttacgtttt tctttcaatg
    58081 gcaaatcacc aatcatttgt ttaagttggt gagggtttac gacaccacta tactggtagt
    58141 catttgaaat tgtttttagg gcttgttcat cgatagatct ctctccagca aatcctttga
    58201 actccgcttc ttttttaata ctttcgaaat taacatatgc ttcatcaata ttgtcatctt
    58261 tatttaatga aggtacaaca ttgtcgataa attctttaat taaatcacgt ttcaatcgta
    58321 aagtcggatc atctgcatgg tctaaaatac gtctaatttg ttctcgatta cgtcgttgtt
    58381 ctgctttatc ttctaaatca atttgcctca atatattcat aatgtaattc acattaatcg
    58441 tatcgttacg catcatttct atttcaaaat caatatcatt taatatggat actttatttt
    58501 tctcagccgt cgatcttttc acttgatcat acacggctaa atatttactt ttataatctt
    58561 cgttttcttg tgcatccata ccaatttcat caacggtaaa ctcaaactca tcaaatgctg
    58621 ttaaacgtaa taaaatttta gctaataaac ggtatgcttc tacaaagcgt ttcagttctt
    58681 cttcatcttg aatatcatca accatgtgtg gtgtcggcac aatcatttta agctcatgat
    58741 acgcttccat aaattctttt ttatactctt cataactgcg cattaaaata gtatccgcat
    58801 catttgtttg tgaaaatact ctcagtgcat cgtccgtctc ttttttcaaa tcgcgatagt
    58861 ttacaatttt accgaacggc ttcgattctt tttcaaccct atttgtacgt gaatatgctt
    58921 gaattaaatc atgatacatt aaactcctat caacatataa agtgttcagt actttactat
    58981 caaatccagt taagaacata ttaacaacga ttaagatgtc aatcttacta tcttttacac
    59041 cttttttaac gttttttgaa atatgattaa aatactcatt atgcgtgtct gttgaaaaat
    59101 tcgtctcaaa ctttttatta taatcactaa tcattttctc taatttttca cgtgaatgat
    59161 atggtacttc accgtcacga tcatcttcat taggtttaaa cgtaaatata ccagctaccg
    59221 ttaatggttg ttctagcttt ttattaagtc gcttaaatgt ctcataatat ttaataagcg
    59281 cgtggatgct ttgtactgta aatatacttg aatattgacg attacgtgtg tatttatcat
    59341 gattattaat gatatgtcgt gctactaatt ccacacgttt atcagctaac catacctctt
    59401 ctgtatcaat tgcttccacc atgctgttat cttctgcttt taaagcttta tttttaaaag
    59461 tattaatata gtcaaccgag aaaccaagta cattaccatc atgtatggca tctctaatta
    59521 aatacgtatg caaacatcta ccaaaaatat ctgcagtcgt acgaccatct tgactactat
    59581 tttcggggaa acgtggtgta ccagtgaatc caaaatattg ggcattttta aaatgttgtt
    59641 taactaaacg atgcatatca ccaaattgac tgcgatgaca ttcatcaata atgaatacta
    59701 ctttatttgt cttatactgc tctaataaat gggcattccc ttgaattgct tttgccattt
    59761 tttgaattgt cgtaacaata agcggtaaac ttttatcatt taattgacgt acgagctgcg
    59821 aagtattaaa agtcttatcg acagcgccct ttgcaaactt attaaattct tcttctgttt
    59881 gactatctaa gtctttacgg tcaaccaaga aaataacttt cttaatactt tcttgttgcg
    59941 acaaaatctg actcgcttta aatgatgtca acgtcttacc gcttcctgta gtatgccaga
    60001 tatatccatt attgcctgtt tcagttgctt gttgtaccag tgcttcagct gcatacactt
    60061 ggtatggacg cattgccatt agaattctat ctgtttcatt aataatcata tagcgtgata
    60121 tcatcttggc taattgacaa ggtctcataa atgtctcagt aaaagattgc aatatattta
    60181 tacgcttatt ttgtttatca ctccaataaa acatatgact ttttaataat tcactgtcat
    60241 tattggaaaa atatcttgtc tcaacaccat tactaatgat aaacatttgt atgtagcgga
    60301 ataagcctgt gtaattttgt ttacggtaac gttttacttg gttaaacgcc tcattaatat
    60361 caatgcctcg acgtttcaat tcaacttgga caaggggtag tccattgatt aatatcgtta
    60421 catcataacg tgctttatat gtatcctcga cagatacttg attcgtcact tgaaacttat
    60481 ttttacacca acttttcgta tctaaaaatg acaaataaat ctcagactca tcatcacgtc
    60541 taagtggtaa tttatcacgt aaaatacggg cactctcgaa aatacttttc ccatcaatca
    60601 tcgttaacag acgttgaaat tctttatctg ttaagggatt gccttctaat ttgtccgcat
    60661 gacgctcatt taaaatcgtt ctaaaattat caagcaattg cttattatca cgtatcgtta
    60721 ctctttcgta acccaattgt tccagttgat tcatcatttc attttctaat gcgtattcac
    60781 tttggtatgc cattcatatc cccttccgta cactttctat tgctctaaat atatcataaa
    60841 ctttaatgaa aaatgtttgt tttttatctt caaacgtaaa tttattctat ttttattgtc
    60901 ttatctttta atatttgtct ttgaggtaag tcgtatacta aaatttgaat acaaataatc
    60961 aaatcattga taaatttttt gtctacgatt aatggaggga cttgaatggt gttaattacc
    61021 tatcaaatca ttttattttt aattattagt ctaagttact atttaacttt aaatcattac
    61081 atggcagtca ctgtaggtaa cttcacttca atattcggca tgttcgcagc catactcttt
    61141 atgtactact acctactcta taaaagtccc gaatacaatc aacgcaaacg atttaaacat
    61201 ttcattcata tcactaattt gataataatt gcttttagca ccttcatatt agttcattta
    61261 gcattaaaat tattcttcag catttaattt ccatctatga aaaaagcaaa gctcaaatct
    61321 gaactttgct ttaatttgtc acgcctttat cattttcaaa atagcctcta tgccagtttt
    61381 acaaacttgt atcaacaatt tttcatcaag caactgaatc acatcaaaaa cttcaattgt
    61441 ttacgttgca actaacttta tattagaacg cacacaatat ctaatgttta aaatctatcg
    61501 tcgcaagatg cttcctgtaa ttatcaagtg ccattttcga ttgggttata caatctagaa
    61561 tcgcatgata atttaatgct acaaatcgat agtacaatat atctaccgtg aataactgtg
    61621 caaatagtga cgttgtagcc gccatacgca tttcattttc atcagttctg ccataaatca
    61681 atgcatagtc tgcaatttga gccactggat tattagctgt actagatata gttatgatgg
    61741 gaatactgta atgtgtggcc acctgtgcaa ttgactgcaa ttcactatga ctaccttgat
    61801 tcgtcacaaa aatcatgcaa tctctatcat catgtgtcgc aaatgttgac acaagtaaat
    61861 gcgtttcatg taataacctg acatttaagc caatacgaga taacttttga aaaagatcac
    61921 caatagtcaa actcgatgcg ccaaatccaa ataaaaatat tgtcctggca tttttcaaca
    61981 catcacaaat tgcatcaatt tgcgcatcca taatattagt agctacaaat cgcatcgtat
    62041 tcgttgctct agcaatcatt ttatttttca aagtttctac agattcattt tcaatcaatt
    62101 ctaaatgtgg attggttgca atatcttcgg gtaagtatcg agatatcgca atctttagct
    62161 cttgaaaacc ttgatgtgtc attttccgac taaatctaac aattgatgct gtactaacat
    62221 tcgtaacatc tgccaaatca ttcacagtca tatcaatgat tttatgtgga ttctttaaaa
    62281 tgtaatcagc gattatcttt tctgtcttcg taaaatcact caactgctta tcaatgcgat
    62341 ataaaatatt tgtcatcatt aatcacccaa caaatctgtc tgtcgcatcg cctttgtcgt
    62401 tccaaataaa tatgtacaaa cgaatccacc agcatacgca gcaagtaatc ctgcaatata
    62461 acctaaatac atattatctg agattaatgg taatagtgac acaccacttg ggcctattgc
    62521 tttggcacca atatgtccaa ttccacctat tacagcgcca ccaataccac cgccaataca
    62581 agcagttaag aaaggtcgac ctaatggcaa agtcacacca tagattaatg gttctccgat
    62641 acctaggaaa ccaactggca atgcaccttt taaagtatta cgtaatgttg tgttgcgttt
    62701 acatcttacc caaagtgcta atgcggcacc tacttgtcca gcaccagcca tcgctgcaat
    62761 tggcaataag taagtagcac cagattggtt aatcatttct atatgaattg gcgtaaaaat
    62821 atgatgaagc cctaacataa ctaatggtag gaagcttgca ccgatgataa atccactaaa
    62881 tacgccacca atactaataa ttccgttaac tactgaaact aaactgtctg aaacaatacc
    62941 tgctaatggc ataaagataa agatagttaa tagtcctaca atcaacaatg caatagtcgg
    63001 cgttacaata atatcaatcg catttggcac aattttatgc aatctctttt cgacaatact
    63061 taaaatccaa acggcaaaaa taacgccaat aattccgcct tgtccaggtt gcaatggttc
    63121 tccagtgaag acattcatta aaatattttt accagcaata cccgttaata acgttgtacc
    63181 accaatcacg ccaccaagtc ctggtgtcgc accaaattct ttagccgcat taataccagt
    63241 gaaaatagct aagtatgcta acataccgtc tttaatgaca ttaaatactg ttataagttg
    63301 cgtaatccaa gcacctgaaa tatagcctgc caccattaag ttactcagta ctgctgcaat
    63361 accaccaatt aatccagctc caataaatgc aggaatcaac ggtataaaga tattagcaat
    63421 tgatttcaat actttattca acttaccatt cttttgtttt gctttatgtg cttctttatt
    63481 cgtctttgct ttatcagctg catatgattt atagtccatt ttttcactat cattgtgatt
    63541 gtgtggtatt gggtcaccta gtttaacacc acttaattcc gccatatgat tagctacttt
    63601 attgactgta ccaggtccaa ccacaacttg aatgcgttca tcgtgtataa cacccatgac
    63661 accatcaatg tgccttagtt cttggtcatc tactttattt tcatctaata ctttaatacg
    63721 cacacgtgtc atacagttca tgacactatc tatattatcc ataccaccta ctgcagcaat
    63781 aattcgttct gcaagttgtt gttctttggt catttaaatc cctcctaagg ttgtctatct
    63841 ctgattgctc gtttaacaat gtcaccattg tttaataacc gtcttgttgc ttcttcctta
    63901 gaaatgccac acatacccat aactgtcgca actttcacat catgctcaga tacctgatat
    63961 aacgccattg cttcatcata tgtgatagca catatttctt gaataatacg cactgaacgg
    64021 tcgatcagtt tttgattggt tgctttaaca tcaatcatta ggttatcgta aacttttccg
    64081 acaccaacca ttgtgatggt tgaaatcata tttaaaatta atttttgtgc tgtaccagac
    64141 tttaaacgcg ttgaaccagt taatacttct ggaccaactt taacttctac tggatactgc
    64201 gcaatttcac ttataattgc atgttcattg catgaaatag atactgttgt agcaccgatt
    64261 gtgttagcaa atgttaaacc gcctataaca tatggcgttt tgccactcgc ggcaattcct
    64321 ataacgacat cttttgatgt taaatctata tttttcaaat cttcttccgc taattttttg
    64381 tgatcttccg caccttctac agccattgtc atagcatgtt gtccaccagc aataatacct
    64441 ataatttcat gagggtcagt attgaatgta ggtacacact ccgctgcatc taagacaccc
    64501 aatcttccac ttgtacctgc accgatatag atcaatcgtc cacccttttt atactgtgca
    64561 attgtttttt taattacttt tgtcaattgt ggtattgcct ttcgaactgc taacgggact
    64621 tgctgatctt ctttattcat cgtaattaaa gcctcttcca cagtcatttc atcaagatgc
    64681 atcgtcgctt cattacgcgc ttcggtcgta ctattttcca tcacttctta cactccctag
    64741 ttttttgaaa atcaaatgta tcattcggct cgatacaact taacagtggt aagtcttctt
    64801 taataatttg tgcaacaaca ttcacattgt catgtgcact aagcgtttgt ctcacaattt
    64861 gcatttcacc ttgataacga ccgttattca aattatcaac ggttactgaa ccaatgcgtc
    64921 gttgcgtcgt aaactgtggt tgaatcgaat gtggacatat ttgtcttgac gtttccgaac
    64981 gaatgacatt ttccggatta tccggacgta ctttatgaca catatcgaaa aggtaagtca
    65041 ctgtcgtatc aaacacttct tcaatacata acgtgaaatg cctatgcttg caaaaatcta
    65101 taagttgttt tgcctgcctc atttcaatca atgagtctcc aactaacact tcagatacac
    65161 cagtttcttg taataattta gctgcaacga caggatgact atgtctcgtt gcttcaattg
    65221 ttggcaagcc tttatgcaaa ggacctcgca aaccactccc tacaataaaa ccatatattt
    65281 gtgcctttgg attaaattga taaatgagtt catttttctt attgaccaag tcaacagata
    65341 atcccgtatc tggtcttgga taatagttat gacaaaatga aagtaatgta aaatcattca
    65401 attgttgatg taagcttgtt aacaattccc gggaaataat acttgcattc aaacagcact
    65461 ttaaaccctg tgccattatc gcttcgattg cctcaattga tgtactatga tcgatacgaa
    65521 tcataaattg tgcatcatat tgtcgaagat gctcataaaa agatggtgtt aatatagatg
    65581 gattagcatc tatgaggtaa gtcacttgtt catgttttaa taaattgagt agttttgtga
    65641 aataatgata ttttgtctcg tcatcttctt ctggtatttg tacagatgta aaaatcattt
    65701 ggtaaccttg tttaatcatt cgcttaatat acgcttcatc taaaggttgt cctaaataca
    65761 ctgaaaagcc tgtcaaagta gccctcctta acaatataat tattaggaaa atatagttga
    65821 gttgtgtaat cgcttacatt ttactataag agaaaacaca ttacaatatt aatcagttga
    65881 agcctgttca ttgtaataat cttacatatt tctgtcacaa gttaattatt acaccatcaa
    65941 agattatcct tttcttttaa gtgctgataa tagctgctac tgctggatta ttacaacaac
    66001 ttttatacat tttattcagg attatcttat attatgtttt aataataatc tgtgaataat
    66061 taagagattt gaaattgaat ttaataattg tattgaaaac gcatacttca ccatgctaaa
    66121 ataggagtcg caaacaaata agattcaata agatgtgatg gtaagctaga taaactaacg
    66181 tatcagtttt atatactact tagtcaattg cttcttttac attaattaca tacaccaacg
    66241 tgttactaag taagattagg catgagttaa attaagctgt gatggttacc aacacagtct
    66301 atttgctcgt gtctttttta ttgaatctta aataataaat acaactttgg aggttggaca
    66361 agtgaggaag aaacttttcg gtcaattgca acgtattggt aaggcactaa tgttacctgt
    66421 tgcaatttta ccagcagccg gtctgttatt agctatcggt acagctatgc aaggtgaatc
    66481 attacaacac tacttgcctt ttatacaaaa tggtggcgta caaactgtcg ctaaattaat
    66541 gacaggtgct ggtggtatca tttttgataa cttgcctatg attttcgcat taggtgtcgc
    66601 aatcggatta gctggcggtg atggtgtagc agctatcgca gcattcgtcg gttacataat
    66661 catgaacaaa acaatgggtg actttttaca agttacacct aaaaacgttg gtgatccagc
    66721 gagtggttac gctagcattt taggtatccc aacattacaa acaggtgtgt tcggcggtat
    66781 tattatcggg gccctggcag cttggtgtta taacaagttc tataacatta acttaccatc
    66841 ttatttaggt ttcttcgctg gtaagcgttt cgtacctatt atgatggcta caacatcatt
    66901 tatattagca ttcccaatgg cattaatttg gccaacgatt caatcaggat taaatgcatt
    66961 cagtacagga ttattagatt caaatactgg tgttgccgta ttcttatttg gtttcatcaa
    67021 gcgtttatta attccattcg gtctgcacca tattttccac gcaccgttct ggttcgagtt
    67081 tggttcatgg aaaaatgcag ctggtgacat tattcatggt gaccaacgta tctttatcga
    67141 acaaattcgt gaaggcgcac atttgacagc tggtaaattc atgcaaggtg aattccctgt
    67201 tatgatgttc ggtttacctg cagcagcttt agcaatttat cacacagcta aacctgaaaa
    67261 caagaaagta gtagccggtt taatgggttc tgctgcttta acatcattct taactggtat
    67321 tacagaacct ttagaattct cattcttatt cgtagcacca ttattattct ttattcacgc
    67381 agtacttgat ggtttatcat tcttaacatt gtacttatta gatcttcatc taggttatac
    67441 attctcaggt ggtttcatcg actacttctt actcggtata ctacctaata agacacaatg
    67501 gtggttagtc attcctgtag gtcttgtata cgcagtcatt tactacttcg tattccgatt
    67561 cctaattgta aaattaaaat acaaaacacc aggtcgtgaa gataaacaat cacaagcggc
    67621 tactgcttca gcaactgaat taccatatgc agtattagaa gctatgggtg gcaaagcaaa
    67681 cattaaacat ttagacgctt gtatcacacg tctacgtgtt gaagttaacg acaaatctaa
    67741 agttgatgtt cctggtttga aagatttagg cgcatctggt gtattagaag tcggcaataa
    67801 tatgcaagca atttttggtc caaaatctga ccaaatcaaa catgaaatgc aacagattat
    67861 gaatggtcaa gtagtagaaa accctactac tatggaagac gataaagacg aaactgttgt
    67921 tgttgcagaa gataaatctg caacaagcga attgagccat atcgtgcatg caccattaac
    67981 tggtgaagta acaccattat cagaagtgcc tgatcaagtg ttcagcgaaa aaatgatggg
    68041 tgacggtatc gctatcaaac cttcacaagg tgaagttcgt gcaccattca acggtaaagt
    68101 acaaatgatt ttcccaacaa aacatgcaat tggtcttgta tcagatagtg gtttagaact
    68161 attaatccac atcggtttag acactgttaa attaaacggc gaaggcttta ctttacatgt
    68221 tgaggaaggt caagaagtta aacaaggtga tttattaatc aactttgatt tagactatat
    68281 ccgcaagcat gcaaagagcg atattacgcc tattatcgtt acacaaggaa acattacaaa
    68341 ccttgatttc aaacaaggtg aacatggcaa catttcattt ggcgatcaat tatttgaagc
    68401 taaataatac ttactaaaac aggtgcgtat gcctttatca ggtgacgtgc ctgttttttc
    68461 tttgctattg tgttttgcag catcattgat agtcgctctc cccttaaatt ttgaatttca
    68521 agatcatcaa ttaacgccct cttcatattc atttcctaaa aaatattaat tgcccgctat
    68581 tgttagcgtt tccacaacaa agtcaacttc cttgacctta cactatattc gaggctatca
    68641 ttttaagtgt aaatataaag aaaaggtggt ttttttatga aacaacgcat tggagcttac
    68701 ttaattgacg ctattcatcg agcaggcgtc gataaaattt ttggtgttcc tggtgatttt
    68761 aatctcgctt ttctagacga tattatcagc aatcccaatg tagattgggt tggaaataca
    68821 aacgaattaa atgcaagtta cgcagcggac ggttatgccc gtcttaatgg actcgctgct
    68881 ttagttacta catttggtgt tggtgaactt agtgccgtca atggtatcgc aggttcttat
    68941 gccgaacgta tacctgtcat tgctattact ggtgcaccaa ctcgtgctgt agaacaagct
    69001 ggcaaatatg tacatcattc acttggtgaa ggaacatttg atgactatcg taaaatgttt
    69061 gctcatatta ctgttgccca aggttacatt acacctgaaa atgcaacaac tgaaatacca
    69121 cgattgatta atactgctat cgccgaaaga cgtccagttc atttacattt accaatcgat
    69181 gtcgcaatct ctgaaattga gatgccgaca ccattcgaag taacaccagc acaacatacc
    69241 gatgcaccaa aatatatcga gttgttaact tctaaattgc agcaagcgaa gcaacctatc
    69301 atcattaccg gacatgaaat taacagtttt catctacatc aagaattaga agattttgta
    69361 aatcaaacac atatacctgt agcacaactt tcacttggaa aaggtgcttt taatgaagaa
    69421 aatccttact atatggggat ttacgatggg aaaattgcag aagataaaat tcgagattat
    69481 gtagacaata gcgatttaat tttaaatatc ggagctaaat taacagattc tgcaacagct
    69541 ggattttcat accaatttaa tattgatgat gtcgttatgt taaatcatca caatatcaaa
    69601 attgatgatg ttacaaatga tacggtatct cttccatcat tgttaaaaca gttatccaat
    69661 gtttcatata cgaacagtgc atcgttccct gtatatcaac gtccaacatc acccgattat
    69721 actgttggca cagaaccatt aacacaacaa acttatttta aaatgatgca aaatttctta
    69781 aaacctaatg atgttattat tgctgatcaa ggcacatcat tctttggtgc ttatgattta
    69841 gcattataca aaaacaatac ttttataggg caaccgttat ggggttctat cggctataca
    69901 ttacctgcaa cattaggttc acaattagca gacaaagatc gtcgtaactt attgttaatt
    69961 ggtgatggct cattgcaact aactgttcaa gctatttcaa ctatgattag acagcatatt
    70021 aaaccggtat tatttgtgat taataatgac ggctatacgg tagaacgact tattcacggc
    70081 atgtatgaac cttataatga aattcacatg tgggattata aagctttacc agctgtattt
    70141 ggtggtaaaa atgttgaaat tcatgacgtt gaatcatcaa aagatttaca agacacgttt
    70201 aatgcaatta atggtcatcc cgatgtgatg cattttgtcg aagtcaaaat gtctgtcgaa
    70261 gatgcaccaa agaaactgat cgatatcgct aaagcttttt cgcaacaaaa taaataattt
    70321 catcacgtta gggtataagt ttaagcgaat actttattaa acgaaaagga ctctgatata
    70381 agatgattaa ttttaataaa accgctttag tgttatcgac ctgcaagaag gtattcttaa
    70441 aatggattat gccccatata cagctgaaaa tgtcgttcaa aacgctaata aattaataga
    70501 tattttcaga aaaaacaatg gttttatcgt attcgttcgt gttaaattct atgatggtaa
    70561 agatgcattg aaaccaaatt cgatggtatc attaccacca aaagaaggcg acgactacag
    70621 tcgtttccat catttattag acaagagaga tggagatttt gtcatagaca aacgacaatt
    70681 tagtgcattt gtaggaacgg atttagactt acagttgaga cgtcgaggaa ttgatacgat
    70741 tgttcttggc ggtgtagcaa cacatgttgg tgtagatacg acagcacgag acgcctatca
    70801 attaaactac gctcagtatt ttgttactga catgatgagc gctcaaaacg aaacgctaca
    70861 tcaattccca atagataatt tattccctat aatggggcaa acagtaacta caagcgacat
    70921 actaaatata ttgaactaag catacactta cccccttcga tcatgttgaa gggggtatct
    70981 ttattttaca aagtattaat acgtcgggtt gtctaacctt ctatatttaa catattctat
    71041 atctgttaaa ccgttcttaa cctacgccct tactacataa aaaaacagta tttattccgg
    71101 aattttcaaa aaatttagta tttattacaa aattatgtat cactttaggt ttaatttttg
    71161 atattatctt aattaagtag atttttataa gttctaa
//
LOCUS       DAHHRR010000002        58911 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.002, whole genome shotgun sequence.
ACCESSION   DAHHRR010000002 DAHHRR010000000
VERSION     DAHHRR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 58911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58911
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.002"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(92..928)
                     /locus_tag="NBI42_000057"
     CDS             complement(92..928)
                     /locus_tag="NBI42_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HCD1776523.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(928..1542)
                     /gene="ureG"
                     /locus_tag="NBI42_000058"
     CDS             complement(928..1542)
                     /gene="ureG"
                     /locus_tag="NBI42_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HCD1776524.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSEVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(1555..2244)
                     /locus_tag="NBI42_000059"
     CDS             complement(1555..2244)
                     /locus_tag="NBI42_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HCD1776525.1"
                     /translation="MIDHKHLRLFQFCDSQFPTGAFSHSFGLETYIQRNVIHDNQTFI
                     AWLKMFLTEQLTYSDGLAMRLVYDALENNDAQKVLNIDKLLFVQNLPKETRVGAKQMG
                     TRMVKLALELYDCQWITWYHQQMKEKNAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQQVVTHMIPYIEATRKHIFELKESDFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(2237..2689)
                     /gene="ureE"
                     /locus_tag="NBI42_000060"
     CDS             complement(2237..2689)
                     /gene="ureE"
                     /locus_tag="NBI42_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HCD1776526.1"
                     /translation="MIIEEIQGNIANLSDSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     NEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDVLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKNLGIPYVHEDRKVNKAFRHIGHSHD"
     gene            complement(2702..4417)
                     /gene="ureC"
                     /locus_tag="NBI42_000061"
     CDS             complement(2702..4417)
                     /gene="ureC"
                     /locus_tag="NBI42_000061"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HCD1776527.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDVIAAEGKIVTAGGIDTHVHFINPEQAEVALESGVTTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSNALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTQNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     IVKGGLINSAVNGDANGSIPTSEPQKYRKMYGQYGGNITSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPNLDVDPQTYEVYVDGEKITSQAATELPLTQ
                     RYFLF"
     gene            complement(4414..4824)
                     /locus_tag="NBI42_000062"
     CDS             complement(4414..4824)
                     /locus_tag="NBI42_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HCD1776528.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVKNTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGDENVNKKGGKRS"
     gene            complement(4838..5140)
                     /locus_tag="NBI42_000063"
     CDS             complement(4838..5140)
                     /locus_tag="NBI42_000063"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HCD1776529.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            5400..6305
                     /gene="yut"
                     /locus_tag="NBI42_000064"
     CDS             5400..6305
                     /gene="yut"
                     /locus_tag="NBI42_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HCD1776530.1"
                     /translation="MKMVDVLLKNISQVVLISNKWTGLFILIGLFVANWTVGLAAMLG
                     SIIAYAFARYINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTVLLSGQVKFVDTSLKLMPQNIETVNFSNNNR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIVSRKAALLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            6522..7676
                     /locus_tag="NBI42_000065"
     CDS             6522..7676
                     /locus_tag="NBI42_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCD1776531.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKAIGDTFKAKSEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLSTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNSTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITNTF"
     gene            7891..8799
                     /locus_tag="NBI42_000066"
     CDS             7891..8799
                     /locus_tag="NBI42_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1776532.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            9200..10141
                     /locus_tag="NBI42_000067"
     CDS             9200..10141
                     /locus_tag="NBI42_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCD1776533.1"
                     /translation="MIKKVYFNHDGGVDDLVSLFLLLQMENVHLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVIGYGP
                     SQGKTFECKDGRKINVINHVDNNAFFNYITALAKKVN"
     gene            10262..10816
                     /locus_tag="NBI42_000068"
     CDS             10262..10816
                     /locus_tag="NBI42_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCD1776534.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGLK"
     gene            10888..11658
                     /locus_tag="NBI42_000069"
     CDS             10888..11658
                     /locus_tag="NBI42_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1776535.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTEERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEKMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(11830..12627)
                     /gene="fdhD"
                     /locus_tag="NBI42_000070"
     CDS             complement(11830..12627)
                     /gene="fdhD"
                     /locus_tag="NBI42_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCD1776536.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPIQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            12917..13699
                     /gene="modA"
                     /locus_tag="NBI42_000071"
     CDS             12917..13699
                     /gene="modA"
                     /locus_tag="NBI42_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1776537.1"
                     /translation="MKMKRFIAIVMALFLVLAGCSNSNDNQDNKKDDAANGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            13713..14384
                     /gene="modB"
                     /locus_tag="NBI42_000072"
     CDS             13713..14384
                     /gene="modB"
                     /locus_tag="NBI42_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCD1776538.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            14385..14990
                     /locus_tag="NBI42_000073"
     CDS             14385..14990
                     /locus_tag="NBI42_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1776539.1"
                     /translation="MLKINLKYQLKNTLIRININDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIE"
     gene            15159..16163
                     /locus_tag="NBI42_000074"
     CDS             15159..16163
                     /locus_tag="NBI42_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCD1776540.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKHRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSACTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDAIHLMNLLFG
                     "
     gene            16195..16701
                     /locus_tag="NBI42_000075"
     CDS             16195..16701
                     /locus_tag="NBI42_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HCD1776541.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTINNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(16703..17197)
                     /gene="moaC"
                     /locus_tag="NBI42_000076"
     CDS             complement(16703..17197)
                     /gene="moaC"
                     /locus_tag="NBI42_000076"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCD1776542.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIYKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTIIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSGQ"
     gene            17260..18519
                     /locus_tag="NBI42_000077"
     CDS             17260..18519
                     /locus_tag="NBI42_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCD1776543.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETTTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQQDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFADGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            18516..19001
                     /gene="mobB"
                     /locus_tag="NBI42_000078"
     CDS             18516..19001
                     /gene="mobB"
                     /locus_tag="NBI42_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCD1776544.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNVVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            19015..19461
                     /locus_tag="NBI42_000079"
     CDS             19015..19461
                     /locus_tag="NBI42_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HCD1776545.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYITMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            19467..19700
                     /gene="moaD"
                     /locus_tag="NBI42_000080"
     CDS             19467..19700
                     /gene="moaD"
                     /locus_tag="NBI42_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HCD1776546.1"
                     /translation="MKVLYFAEIKDILQKAQEDIMLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            19707..20306
                     /gene="mobA"
                     /locus_tag="NBI42_000081"
     CDS             19707..20306
                     /gene="mobA"
                     /locus_tag="NBI42_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCD1776547.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            20326..21348
                     /gene="moaA"
                     /locus_tag="NBI42_000082"
     CDS             20326..21348
                     /gene="moaA"
                     /locus_tag="NBI42_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCD1776548.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDLLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(21801..21971)
                     /locus_tag="NBI42_000083"
     CDS             complement(21801..21971)
                     /locus_tag="NBI42_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776549.1"
                     /translation="MFRFISFGQLVVYSTTFVPKKDILQLNDMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            22097..22447
                     /gene="sarV"
                     /locus_tag="NBI42_000084"
     CDS             22097..22447
                     /gene="sarV"
                     /locus_tag="NBI42_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HCD1776550.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(22549..23760)
                     /locus_tag="NBI42_000085"
     CDS             complement(22549..23760)
                     /locus_tag="NBI42_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1776551.1"
                     /translation="MINQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLIYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAINLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(23753..24193)
                     /locus_tag="NBI42_000086"
     CDS             complement(23753..24193)
                     /locus_tag="NBI42_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HCD1776552.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKY
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYGI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLKEAHND"
     gene            24356..25126
                     /locus_tag="NBI42_000087"
     CDS             24356..25126
                     /locus_tag="NBI42_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1776553.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDDDEIKPPFFIQWEESDSMRI
                     KKLQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFR
                     IEDGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(25118..25369)
                     /locus_tag="NBI42_000088"
     CDS             complement(25118..25369)
                     /locus_tag="NBI42_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776554.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLAIIHMGILAHSTYVYLY"
     gene            25758..27023
                     /gene="femX"
                     /locus_tag="NBI42_000089"
                     /gene_synonym="fmhB"
     CDS             25758..27023
                     /gene="femX"
                     /locus_tag="NBI42_000089"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HCD1776555.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEIQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKELAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            27140..30304
                     /locus_tag="NBI42_000090"
     CDS             27140..30304
                     /locus_tag="NBI42_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCD1776556.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASANNISGMPTVKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQV
                     AKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLRN
                     IRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYRR
                     LTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAIA
                     FSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWIV
                     IILSTLILVATVVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAQDVEKYL
                     KQKKHVKTIQYSVGGSSPVDPTGSTNSMVIMVEYDNDTPNFDVEADKVIKHADGFKHP
                     GEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTYD
                     QYEIKVDQNKAAENGISASQLAMHLNENLPEKAVTTVKENGKTVDVKVKQNKQTDWSE
                     DKLNNITLKKPTGDTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTTR
                     QVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLAP
                     FTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQG
                     MEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISST
                     LLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(30491..30808)
                     /gene="mspA"
                     /locus_tag="NBI42_000091"
     CDS             complement(30491..30808)
                     /gene="mspA"
                     /locus_tag="NBI42_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HCD1776557.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLAGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(31053..31217)
                     /locus_tag="NBI42_000092"
     CDS             complement(31053..31217)
                     /locus_tag="NBI42_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776558.1"
                     /translation="MSLENQLAELKYDYVRLQGDLEKRESLNLDTSALVRKLKDIENE
                     IRNVRAQMQD"
     gene            31350..32258
                     /locus_tag="NBI42_000093"
     CDS             31350..32258
                     /locus_tag="NBI42_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCD1776559.1"
                     /translation="MTEQFTMIILLIALGYFLKRINYIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFGMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVHVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     PFIGMTTNLTIIISIIILYVYQAVFV"
     gene            32429..32560
                     /locus_tag="NBI42_000094"
     CDS             32429..32560
                     /locus_tag="NBI42_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776560.1"
                     /translation="MYGAYLINEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(32677..33540)
                     /locus_tag="NBI42_000095"
     CDS             complement(32677..33540)
                     /locus_tag="NBI42_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HCD1776561.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(33721..34608)
                     /locus_tag="NBI42_000096"
     CDS             complement(33721..34608)
                     /locus_tag="NBI42_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1776562.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVETFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            34674..34769
                     /locus_tag="NBI42_000097"
     CDS             34674..34769
                     /locus_tag="NBI42_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776563.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            34795..36930
                     /locus_tag="NBI42_000098"
     CDS             34795..36930
                     /locus_tag="NBI42_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCD1776564.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDENLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPLRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYYTLSL
                     TVKGFDFQLESNQRYTNKETLEQIVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            37043..38377
                     /locus_tag="NBI42_000099"
     CDS             37043..38377
                     /locus_tag="NBI42_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCD1776565.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(38569..38958)
                     /locus_tag="NBI42_000100"
     CDS             complement(38569..38958)
                     /locus_tag="NBI42_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776566.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            39315..39623
                     /gene="rpsJ"
                     /locus_tag="NBI42_000101"
     CDS             39315..39623
                     /gene="rpsJ"
                     /locus_tag="NBI42_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCD1776567.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            39651..40313
                     /gene="rplC"
                     /locus_tag="NBI42_000102"
     CDS             39651..40313
                     /gene="rplC"
                     /locus_tag="NBI42_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCD1776568.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            40340..40963
                     /gene="rplD"
                     /locus_tag="NBI42_000103"
     CDS             40340..40963
                     /gene="rplD"
                     /locus_tag="NBI42_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCD1776569.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            40963..41238
                     /gene="rplW"
                     /locus_tag="NBI42_000104"
     CDS             40963..41238
                     /gene="rplW"
                     /locus_tag="NBI42_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCD1776570.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            41271..42104
                     /gene="rplB"
                     /locus_tag="NBI42_000105"
     CDS             41271..42104
                     /gene="rplB"
                     /locus_tag="NBI42_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCD1776571.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            42171..42449
                     /gene="rpsS"
                     /locus_tag="NBI42_000106"
     CDS             42171..42449
                     /gene="rpsS"
                     /locus_tag="NBI42_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCD1776572.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            42478..42831
                     /gene="rplV"
                     /locus_tag="NBI42_000107"
     CDS             42478..42831
                     /gene="rplV"
                     /locus_tag="NBI42_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCD1776573.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            42855..43508
                     /gene="rpsC"
                     /locus_tag="NBI42_000108"
     CDS             42855..43508
                     /gene="rpsC"
                     /locus_tag="NBI42_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCD1776574.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            43511..43945
                     /gene="rplP"
                     /locus_tag="NBI42_000109"
     CDS             43511..43945
                     /gene="rplP"
                     /locus_tag="NBI42_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCD1776575.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            43935..44144
                     /gene="rpmC"
                     /locus_tag="NBI42_000110"
     CDS             43935..44144
                     /gene="rpmC"
                     /locus_tag="NBI42_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCD1776576.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            44168..44431
                     /gene="rpsQ"
                     /locus_tag="NBI42_000111"
     CDS             44168..44431
                     /gene="rpsQ"
                     /locus_tag="NBI42_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCD1776577.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            44463..44831
                     /gene="rplN"
                     /locus_tag="NBI42_000112"
     CDS             44463..44831
                     /gene="rplN"
                     /locus_tag="NBI42_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCD1776578.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            44867..45184
                     /gene="rplX"
                     /locus_tag="NBI42_000113"
     CDS             44867..45184
                     /gene="rplX"
                     /locus_tag="NBI42_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCD1776579.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            45211..45750
                     /gene="rplE"
                     /locus_tag="NBI42_000114"
     CDS             45211..45750
                     /gene="rplE"
                     /locus_tag="NBI42_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCD1776580.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            45773..45958
                     /locus_tag="NBI42_000115"
     CDS             45773..45958
                     /locus_tag="NBI42_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCD1776581.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            45990..46388
                     /gene="rpsH"
                     /locus_tag="NBI42_000116"
     CDS             45990..46388
                     /gene="rpsH"
                     /locus_tag="NBI42_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCD1776582.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            46413..46949
                     /gene="rplF"
                     /locus_tag="NBI42_000117"
     CDS             46413..46949
                     /gene="rplF"
                     /locus_tag="NBI42_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCD1776583.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            46980..47339
                     /gene="rplR"
                     /locus_tag="NBI42_000118"
     CDS             46980..47339
                     /gene="rplR"
                     /locus_tag="NBI42_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCD1776584.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            47360..47860
                     /gene="rpsE"
                     /locus_tag="NBI42_000119"
     CDS             47360..47860
                     /gene="rpsE"
                     /locus_tag="NBI42_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCD1776585.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            47877..48056
                     /gene="rpmD"
                     /locus_tag="NBI42_000120"
     CDS             47877..48056
                     /gene="rpmD"
                     /locus_tag="NBI42_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCD1776586.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            48073..48513
                     /gene="rplO"
                     /locus_tag="NBI42_000121"
     CDS             48073..48513
                     /gene="rplO"
                     /locus_tag="NBI42_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCD1776587.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            48513..49805
                     /gene="secY"
                     /locus_tag="NBI42_000122"
     CDS             48513..49805
                     /gene="secY"
                     /locus_tag="NBI42_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCD1776588.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            49822..50469
                     /locus_tag="NBI42_000123"
     CDS             49822..50469
                     /locus_tag="NBI42_000123"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCD1776589.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            50662..50880
                     /gene="infA"
                     /locus_tag="NBI42_000124"
     CDS             50662..50880
                     /gene="infA"
                     /locus_tag="NBI42_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCD1776590.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            50912..51025
                     /gene="rpmJ"
                     /locus_tag="NBI42_000125"
     CDS             50912..51025
                     /gene="rpmJ"
                     /locus_tag="NBI42_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCD1776591.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            51048..51413
                     /gene="rpsM"
                     /locus_tag="NBI42_000126"
     CDS             51048..51413
                     /gene="rpsM"
                     /locus_tag="NBI42_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCD1776592.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            51437..51826
                     /gene="rpsK"
                     /locus_tag="NBI42_000127"
     CDS             51437..51826
                     /gene="rpsK"
                     /locus_tag="NBI42_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCD1776593.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            51901..52845
                     /locus_tag="NBI42_000128"
     CDS             51901..52845
                     /locus_tag="NBI42_000128"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCD1776594.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            52862..53230
                     /gene="rplQ"
                     /locus_tag="NBI42_000129"
     CDS             52862..53230
                     /gene="rplQ"
                     /locus_tag="NBI42_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCD1776595.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            53768..54577
                     /locus_tag="NBI42_000130"
     CDS             53768..54577
                     /locus_tag="NBI42_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCD1776596.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQTITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPHDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDHAEELTTIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            54574..55434
                     /locus_tag="NBI42_000131"
     CDS             54574..55434
                     /locus_tag="NBI42_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCD1776597.1"
                     /translation="MTIRFVNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLKDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKTIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKEKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            55424..56230
                     /locus_tag="NBI42_000132"
     CDS             55424..56230
                     /locus_tag="NBI42_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD1776598.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFFMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTLSLIMIIPIAIILFVLKYSGV"
     gene            56235..57038
                     /gene="truA"
                     /locus_tag="NBI42_000133"
     CDS             56235..57038
                     /gene="truA"
                     /locus_tag="NBI42_000133"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCD1776599.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLDKMNRAAQQFKGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLLAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            57278..57715
                     /gene="rplM"
                     /locus_tag="NBI42_000134"
     CDS             57278..57715
                     /gene="rplM"
                     /locus_tag="NBI42_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCD1776600.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            57729..58127
                     /gene="rpsI"
                     /locus_tag="NBI42_000135"
     CDS             57729..58127
                     /gene="rpsI"
                     /locus_tag="NBI42_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCD1776601.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            58742..>58911
                     /locus_tag="NBI42_000136"
     CDS             58742..>58911
                     /locus_tag="NBI42_000136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776602.1"
                     /translation="MTLTVDLKKQPYEVFDLTESDCLHLKNKNFIFGKNGRGKSTLTK
                     MIENEYREGYNI"
BASE COUNT        20924 a         8891 c        11053 g        18043 t
ORIGIN      
        1 aatattgaag atgctttaat taaagttaaa gactagccat accttatttc agcttattaa
       61 gcttgaaaca aggtacacta gtctttttat tttaatattt tcttagaaaa tcaagtttac
      121 gatcataaat attttctgcg atatagcttt gtatggttcc aagtattttc tctataattt
      181 gtgtgcgata agcaaaaatt ctaactgcaa aaccatgtgt aggcaattga gaaatagcaa
      241 cacgacaatc gaatgtattg ctataagaac taatggtttc gtaaactgaa tcgattagtt
      301 tttggttaac atcttcatgt atgaagtaag cagagccata atgagagtaa tgttccatat
      361 aacctatttc attgatcgat tgtttattag gattcaataa taaattatca tatgtgacta
      421 actcatcttc aatataaatt tcatttatta aatgcatata ttgatacttg aaggcttcgc
      481 cagtttttga ataaccagga gttaaaatat cagtataaaa taatgaacta gaattattga
      541 gattgaacgt attgtgttga taaaatttag cattttcata tgcgatgatt ggatcagcga
      601 catattctaa atatgcgtta tctttcaaat taaaagtttg atactgctca acatgattgg
      661 aaggtgtctt gtatattttt gttgcacctt gagatgtcaa tgtcacttta gcgttatctt
      721 cgacattcac attcatacgg taacgatctc catctaaata accaccacct acattcacta
      781 tataaaatgt aggaatggta gattgattta gataaactgg acgtatcact ttaagtgctt
      841 tttcaaagaa aatatctctt gatactgatc gattgccatc aaaaaacact gttaaatcaa
      901 gttgcccagt ccattgttgt tcatccatta tgataatcct ttgagtaaag tgtcgcgttc
      961 aatccaatcg ataacttcat ccaaaccttc gtcggttttt aaattagtaa aagtaaatgg
     1021 acgtttacca cgaaatactt tagtatcttc agccatttgt tctaatgatg caccaacata
     1081 gggagctaaa tcagttttgt taattacaaa gaaatctgac ttaatcatac cttgaccacc
     1141 tttacgtgga atcttttcac cttgagcaac atcgataata taaattgaaa agtcaacaag
     1201 ttctggacta aatgttgctg ctaagttatc gccaccagat tctatgaaaa ttagttctat
     1261 atcgtcatga cgttctaata attcgtctat tgctgcaaag ttcatagatg catcttcacg
     1321 aatcgcagta tgaggacatc caccagtttc aacaccaatg atacgacttt caggtagaac
     1381 ttctgaattt actaatatct tttcgtcttc ttttgtatat atatcatttg taataacacc
     1441 gatactcatt tcttttgaaa gacgttttac aactttttca attaattgtg ttttacctgc
     1501 gccaacaggt ccaccaatac caattttaat cggatttgcc acaattataa cctcctatga
     1561 tatgaaaatt ctaacattga cgttttcatg cgccatttgg tttagttcta agccaggtgc
     1621 tgtcatgccg aaatcagact cttttaattc gaaaatatgt tttctcgttg cttcgatata
     1681 aggaatcata tgagtaacca cttgctgacc agcagtttgt ccaagtggga tggcacgaac
     1741 agcattttga gttaaacttg aaacattttg atataagtaa taatcaataa ttgtttcaat
     1801 atctacacct aaatggtgtc ctaacatcgt aaaacatatt gctggattta attttgcatt
     1861 tttctctttc atttgttgat gataccaagt tatccactgg caatcatata gttctaatgc
     1921 aagtttaacc atacgcgtac ccatttgttt tgcaccaaca cgcgtttctt taggtaaatt
     1981 ctgaacaaat aatagtttgt ctatatttaa tactttttgc gcgtcattat tttctaatgc
     2041 atcataaact aatcgcattg ctaaaccatc agaataggtg agttgttctg ttaaaaacat
     2101 ttttagccat gcaataaaag tttgattatc atgaatcaca tttctttgaa tgtatgtttc
     2161 aagaccaaac gaatgactga atgcgcctgt tggaaactgt gaatcacaga actgaaataa
     2221 tcttaaatgc ttatgatcaa tcatgagaat gtcctatatg tctgaaggct ttattaactt
     2281 tacgatcttc atgaacatat ggaataccta gattttttaa taaatcttct actaaataat
     2341 cgtattgtac taacatttca gtttctgtaa attgtgcggg taaatggcga tttcctagtt
     2401 gatgtgcaat gtcacccatt tcttgtagtg ttcgtggttg aatgactaag acatcttctg
     2461 aatttacatc aacaataatc atattatgat catccgcata taaaatatct ccgtattgca
     2521 agtcgattgg ttgttttaaa cggataccaa tttcattacc gtgatctgta acaactcttt
     2581 gaatacgttt aacaaggtct gagttttcaa gatacacttt ttcaacatgc ttttgctttt
     2641 ctgaatctga taaattggca atattgcctt gaatttcttc tataatcatt ttatgttcct
     2701 cctagaataa gaagtatctt tgagttaatg gtaactctgt tgctgcttga cttgtaattt
     2761 tttcaccgtc tacatatact tcatatgttt gcggatcaac atccaaatta ggggtagcac
     2821 tattattttt catgtctgct ttagataatt gtctaatatt tttaacaggg cggaccatgc
     2881 gttttaagtt taatgcacgg ttaataccat tttcatatgc agttttagat acgaatgtca
     2941 ttgatgtgct agtaatgtta ccaccatatt gaccgtacat tttacgatat ttctgtggtt
     3001 cggatgtagg aatagaacca ttggcatctc cgttaacggc tgaattaatt aaaccacctt
     3061 ttacaatcaa ttcgggttta acaccgaaga aaattggatc ccataaaacg atgtcagcaa
     3121 gtttgcctgg ctcgatagaa cctacatatt cagaaatacc atgtgtaata gctgggttaa
     3181 ttgtatattt agcaatataa cgtttaatac gattattatc attatgttca aagtcgccat
     3241 ctaatggacc acgttgttct ttcatacggt gtgccacttg ccatgttctt gtaatcactt
     3301 cacctacacg gcccattgcc tgtgaatcag aactaatcat actaaataca cccatatctt
     3361 gaagtacatc ttctgctgcg attgtttctt ttcgaatacg tgagtcagca aatgcaatat
     3421 cttctggaat agctgcattt aaatggtgag taatcataac catatctaaa tgttcatcta
     3481 ctgtattttg cgtatatggt aatgttggat tcgtagatga aggtaatata tttgaaaatg
     3541 ctgctgattt aattaaatca ggggcatgtc cgccaccagc accttcagta tgatacatgt
     3601 gtagtacacg gtcttttact gctgccatcg tatcttccat aaaacctgct tcatttaaag
     3661 tatctgcatg taaagcaatt tgaacatcaa attcatcagc aacatctaaa gcgttgctta
     3721 aagctgatgg tgttgcaccc cagtcttcat gtacttttaa accaattgca cctgcattaa
     3781 tttgttcgat aagtgcagtt gggttagttg cttgtccttt accagtaaag ccaacattga
     3841 ttggtaaacc ttctgcagct tctaacattc tatgaatatg ccaaggacca ggtgttacag
     3901 tagtagcttt cgatccctct gaagcaccag taccgccgcc aatatgcgtt gtaacaccac
     3961 tttcaagtgc aacttctgct tgttcagggt tgataaaatg aacgtgagta tctataccac
     4021 cagcagttac gattttacct tctgctgcaa taacatctgt agtagaacca ataataatgt
     4081 cgacattatc cattatatct gggttgccgg cattaccgat ggcaaagata taaccgtttt
     4141 taatacctat atctgctttt acaactttat cgtaatcgat aataacggca ttagaaataa
     4201 ctaagtctgc tacattaaca tcatcacgtg ttacacgagg attttgagcc ataccgtcac
     4261 ggatagattt accaccacca aaggtagctt cttcaccata aactgcatag tctttttcta
     4321 tttgagcaaa tagattcgta tcacctaaac gaatagaatc tccaacagta ggaccgtata
     4381 agctcgtata ttgattttgc gtcattttaa agctcatgat cttttccctc cttttttatt
     4441 cacgttttca tcaccgttat ctccgaatac acctgcatat tcatcatttt catcagttgg
     4501 gcgatagaca cgtgactcat cgataggacc attgaccata ccacgaaaac caaaaatttt
     4561 acgtttgcca gcatattcaa ctaattgaac ttctttttta tccccaggtt caaatcgaac
     4621 agctgctcca gctggaatat ctaaatgttt tccatatgcc atttcacgtt cgaaatctaa
     4681 tgctgcattt gcttcataaa aatgaaaatg tgagcccact tgaattggtc gatctcctgt
     4741 atttttaact tcgataactg tttcaggatg atggttatta atttcaacct ctgtactttt
     4801 tgtaataatt tctcctggta tcatttgact gcctccttta aacaataggg tgatgtactg
     4861 tgattaactt agtaccatcg gggaacgtag cctcgatttc gatatctgta atcatgtgtt
     4921 cgacaccatc catgacatct tctttgttta gaatttgtct accataactc attaactctg
     4981 caacggtctt accatcgcgt gcaccttcta ataattcatc gctgattaaa gctaatgcct
     5041 cagggtgatt tagtttcaaa ccacgtgctt tacgacgacg tgcaacttcc gccgccacta
     5101 caatcattaa tttgtcttgc tctcgttgtg taaaatgcaa attaaaaccc ccaatttcat
     5161 attagataca atttacaaaa tttatattaa tcctaattgt tgtgataaac aagtaatata
     5221 caaagtttaa agcggaacag taaaaaataa atttactatt tcgtatttaa aaatatatga
     5281 ttatgattaa aacaaatagc gaaagatatt tagggtttga gaaatagagt ttttaatata
     5341 actttttgta aaatggtagt atattaatga gtaaacattc aagaaaaagg gtgggcattt
     5401 tgaaaatggt agatgtgctt ttgaaaaata tatctcaagt agtgttaatt agtaataagt
     5461 ggactggttt gtttatcttg attggattat tcgtggctaa ctggacagtt ggattagcag
     5521 ctatgctagg tagtatcata gcttatgcat ttgcacgtta cattaattat agtgaagcag
     5581 agattaatga tgggttagcc ggatttaatc cagtgttaac tgccattgcg ttaacaatat
     5641 ttttagataa gtcaggatta gatattgtta taacaatgat agcaacttta ttaacgttac
     5701 cagttgctgc tgcagtgaga gaagttttaa gaccatataa agttccgatg ctgacgatgc
     5761 cttttgtcat tgtgacttgg tttacagttt tactttcagg acaggttaaa tttgtagata
     5821 catcgttaaa gttaatgcct caaaacattg aaacggttaa ttttagcaac aataatcgaa
     5881 tacatttcat tcagtcatta tttgagggat tcagtcaagt atttatcgaa gcgagtgtaa
     5941 ttggtggcgt atgtatttta atcggcatat tgatagtatc aagaaaagca gcactcttag
     6001 ctgttatagc tagtttgtta agctttatca ttgtagctct attaggtggt aattatgatg
     6061 atattaatca gggattattc ggatataact ttgtattaat ggcaatcgca ctaggatata
     6121 catttaaaac agcgattaac ccttatattt cgaccttttt gggtgtgtta ttaacagtag
     6181 tggtgcaact aggtacaaca acattacttg aaccgtttgg cttacctgca ttaacattgc
     6241 catttattat tgtgacatgg attttattat ttgctggtat taaacatgac aaagtagatg
     6301 cttgatagtt aaatcaaacc tagtattgtt tgaatatcac cttaaaccat acagcgaatt
     6361 gtatagttta aggtgtattt ttatggagaa aattaagtgc atacttaatc gaattatcag
     6421 ctttctttta attgttcaca gcacgttcct ttttattttt aaagtcgagt gttaaactag
     6481 gaataaataa tttatattgt gtgttgtgtg gggtgactaa tatgaatgat atggataatt
     6541 cctttttaat aacaacggaa attcaaagga aatggattga aaaattcaaa gcaattggag
     6601 atacatttaa ggctaagtct gaatataatg atcaacatag ccaatttcca tataaaaata
     6661 ttgaatggtt aattaaagaa ggttatggaa aattaacgtt accaaaagca tatggtggcg
     6721 aaggtgcgac catagaagac atggttattt tgcaatcatt tttaggcgaa cttgatggtg
     6781 ccacagcatt atctattggt tggcatgtga gtgtcgtagg acaaatttat gaacagaaat
     6841 tatggtctca agatatgttg gagcaatttg ctgttgaaat taataatggt gcattagtta
     6901 atagagcagt tagtgaagct gaaatgggta gtccaacaag agggggaaga ccaagtacac
     6961 atgctgttaa agctgatgat gggtatattt taaatggtgt gaagacatat acatcaatga
     7021 gtaaagcgct aacacatatt attgttgctg cttatataga agaattagaa agtgttggtt
     7081 ttttcttagt ggacaggaat ttgcctggtg ttgaaattgc cgataattgg gacgtattag
     7141 gtatgcgagc gacagaaagt catgatttaa tattaaatga tgtaaaagta ccattaaaac
     7201 atttagtgga aacagagaaa agtaaagcgc caaatggttg gatattgcat ataccaagct
     7261 gttatttagg tatagcacaa gctgctagaa attatgctgt agactttgca atccaacata
     7321 gccctaatag tattgaagga acgattgcaa cattgtcaac tgtgcaacaa aatttaggga
     7381 aaatggaaac gctattgtta tgtgcgagac aatttttatg gagtacagca aaaggctatc
     7441 aacaatataa agatgacagt cagataagaa attcaacaag tgcgagtaaa gtgatggtaa
     7501 tgaatcaagg gcttgaagtg attgatttag cgatgagaat agttggtgct aaaagcttag
     7561 aaatgaatcg accattacaa cgttactatc gtgacatgcg tgcaggatta cacaatccac
     7621 ctatggaaga tgcagcatat acaaatattg ctaagagtat tacaaataca ttttaaattt
     7681 aaaaaggtaa atcgatcgtt gattggcata aagagatgac atttgacgtt cggatttacc
     7741 ctttttttgt gggaatttat agggatgtta agatattttg ctaaattttt tgcgaaaata
     7801 aaaaaataaa tagcactagc tattgtaaat gtatatgcaa tcttgtataa ttttggaata
     7861 attgataatc attttcaata ggaggaaatt atgaaaaaac tattattacc attaataatt
     7921 atgttattag tgttagctgc gtgtgggaac caaggtgaaa aaaataacaa agctgaaact
     7981 aaatcttata aaatggacga tggcaaaacg gtagatattc cgaaagaccc taaacgcatt
     8041 gcagtagttg cgccaacata tgctggtgga cttaaaaaat taggtgcaaa cattgtagct
     8101 gtaaatcaac aagtcgatca aagcaaagta ttaaaagata aatttaaagg tgttacaaaa
     8161 attggtgatg gcgatgtaga aaaggttgct aaagaaaagc cagatttaat tattgtatac
     8221 tctactgaca aagacattaa aaaatatcaa aaagtagcac caacagtagt tgttgactat
     8281 aataagcata aatatttaga acaacaagaa atgttaggga aaattgttgg taaagaagat
     8341 aaagtaaaag cttggaagaa agattgggaa gaaacaactg ctaaagacgg taaagaaatt
     8401 aaaaaagcaa ttggacaaga tgcaacagtg tcattgtttg atgaatttga taaaaaatta
     8461 tacacttacg gcgataactg gggtcgtggt ggagaagtat tatatcaagc atttggttta
     8521 aaaatgcaac cagaacaaca aaagttaact gcaaaagcag gttgggctga agtgaaacaa
     8581 gaagaaattg aaaaatatgc tggtgattac attgtgagta caagtgaagg taaacctaca
     8641 ccaggatatg aatcaacaaa catgtggaag aatttgaaag ctactaaaga aggacatatt
     8701 gttaaagttg atgctggtac atactggtac aacgatcctt atacattaga tttcatgcgt
     8761 aaagatttaa aagaaaaatt aattaaagct gcaaaataat tcagctatat aagtcagtga
     8821 aatgagagtc tgaaacatat caattttttg atattgtatt aggctctcat ttttatagca
     8881 agaaagttag agttttgtat tttttttaaa ataataagtg ccgttgttat cattcaattt
     8941 aattaatgat agattagtat tattacagct aaagtagtat accagagtaa atagctcaat
     9001 gtatctcttc attaataagt tatatcataa ttattttagt gcatacttta tggaagccat
     9061 atcagggaat ggttatcatt taaagaagag gtttaaaagg attacaacag aatgttatga
     9121 ttttgtagaa agatatataa caacgtttta taaaaacata atattgttaa tggaaaatga
     9181 aatgtaaggg ggatttcgag tgattaagaa agtttatttt aaccacgatg gtggtgtaga
     9241 tgatttagta tctctatttc tattattaca aatggaaaac gttcatttga taggggtcag
     9301 tacaattggt gctgattgtt atttagagcc atctttgagc gcatcagtaa aaattattaa
     9361 tcgtttttca aatgaagata ttcaagttgc gccatcatat gaacgaggaa aaaatccatt
     9421 tcctaaagaa tggcgtatgc atgccttttt tatggacgca ttgccaattt taaatgagcc
     9481 agtcaaacac gttgcttcaa atgtgagcga caaagaagcc tttgaagaca ttattcaaac
     9541 tttaaagaga caatcagaaa aagtaacatt attatttaca ggcccgctta cagatttagc
     9601 aaaagcacta caaaaagatt catcaatcgt tcagtatata gaaaaattag tttggatggg
     9661 tggtaccttt ttaccaaaag gaaatgttga agaacctgag catgatggtt ctgcagaatg
     9721 gaatgcatat tgggatccag aagcagttaa aattgttttt gatagcgata tagagattga
     9781 tatggttgct ttagaaagta cgaatcaagt accgctaacg ttagatgtta gacaaagatg
     9841 ggcaaatgaa cgccaatata cgggcattga ttttttagga gtaagttatg cagcggtacc
     9901 accattaaca cactttataa caaattctac atacttttta tgggatgttt taacgactgc
     9961 ttatattggt aacaaggact tggttcattc tattgagaag aaagtagatg taataggtta
    10021 tggcccaagt caaggtaaga catttgagtg taaagatggg cgcaaaatta atgtcataaa
    10081 tcatgtagat aacaacgcat tttttaatta tataactgca cttgctaaaa aagtaaatta
    10141 acagctgtgt agaataatta atgttttaat ttatatagaa caacttattg taaacttttc
    10201 gtttcttaaa gtttacaatg gtgctataat aatggtcatg aaatacgaaa ggaagtaaaa
    10261 aatgacaaca aaacagttag tatatacagc tttaatgaca gcgattatcg ctattctagg
    10321 attggtaccg gtaattccac taccattttc ttcagtacca attgtacttc aaaatattgg
    10381 tattttctta gcaggtgtga ttttaggacg taaatatggc acattaagtg ttatcgtctt
    10441 tttattatta gtagttgctg gcttgccact gttatcaggc ggtcgcggtg gcattggtgt
    10501 attcgcaggt ccttcagcag ggtttttact attatatcca gttgtagcat tcatgattgg
    10561 tgcgattcgt gatagattca tcaatgaaat taatttctgg attttattcg ttggtatttt
    10621 agtttttggt gttatagcat tagatgttat tggtacattg attatgggca tgattattaa
    10681 cataccattt acgaaagcta tttcaatttc attagcttat ttacctggtg atatattaaa
    10741 agcaattgta gcaagtttga ttggtacagc tttacttaat cactcgcagt ttcgtcaaat
    10801 tatgggatta aaataatcat atttaagata ataaagtaat tgaataagtt gctttgaatt
    10861 ttataaaagt gaaaggagta ggtgtcaatg gctagtataa gtatgtcaga tatatattgt
    10921 aacggcacta tatttgaaaa tgacgacgag cagttgattt atttaacgcc ttcttttcca
    10981 caacgataca caagcaacac atggatatat aaaaagacgc ctaccgaaga gcgatggctg
    11041 aaagacttag aacgtcaaca tcaattacat acaaatcaag gttcaaatca ttatgcgttt
    11101 agtttcccgg aaaatgaaca acttgataat cattggatgg ctatgtttaa agatatgaat
    11161 tttgaactag gtattatgga attgtatgcc atagaaagtg atgcgcttgc taatttgcca
    11221 cgtaactctg acgttgaaat tgccatcgtt gacgagtcgc atatagatgc ctatttaaaa
    11281 gttgcatatc aatttagttt gccatttgga aaagactatg cagatgcaca tgaaaaaatg
    11341 gtaagggaac attatcaaaa agatgtgatt aaacgcttag tagcttattt aaataatgaa
    11401 cctattggcg ttgtagatgt cattgaaagt gaaaattaca ttgaattaga tggatttggt
    11461 gtattagaac aatttcggca ccaaggaatt ggatctacaa ttcaatcgtt gataggtgaa
    11521 tacgccatat caaaaaatca caaaccaatc atattagttg cagatggtga agatacagca
    11581 aaagatatgt atgcaaagca aggttatgtt taccaatcat tttgttatca aatattaaaa
    11641 gaagatattg gaaattaacg attgatgata ggtttgtatt agaataaaaa agcagtaagc
    11701 tggtttcgag tagaaatcaa cttactgctt tttaaattgt tttgagctac ttatacttat
    11761 aaaaatagtg cttttatatt gttgattcat gtagaatatc gttcattatg tcacactaaa
    11821 atgaatatgt tattgttcag aatcaatgat acgttctgga tgactgtata tattaaagcc
    11881 accatttcga ataaatccaa ctgccgtaat atttaggtca ttagctaagg ttacagcaag
    11941 cgttgtcgga gccgatttag ataaaatgac gccaacacca attttcgcgg ctttaattaa
    12001 aatttctgat gaaatacgtc cactgaaaat taatacttta tctcggacag taatatgtcg
    12061 ctgaatacaa aatccatata atttatctag agcgttatgt ctaccaatgt cttgtcgatg
    12121 tacaaaaaat gtcaaaccat cgcttatagc agcattatgt aagccacctg tttcttggta
    12181 aatatgactt gcactttgta atcgagtcat catgtttata atttgtattg gagttaaagt
    12241 gattttagac atagatgttt tagcgatagc agcatcattt tgaaaataaa actcacgact
    12301 ctttccacaa caagatgcaa tcattcgttt tgtggaatat tgaaagcgat cgcctaaatc
    12361 tttattaagt tcaacatggg caaatccttt actatcatca atcagtacag attttaattc
    12421 atctcgcttt aaaatggcac cttccgaagc cagaaatcca atgactaact cctcaaggtt
    12481 tgttggactg catataacag tcgcaaattc ttcaccattc accataattg taagtggaaa
    12541 ttctgtcaca tattgatctg ttgtattgaa taattttcca tcttcatatc taacaattgg
    12601 ttgacctaaa gatacatctt tgttcattat ctaacccctt taattagctt gaactttatt
    12661 ttaaagcaat ttgcttagaa ttttaacata tttgcttaag tttgaaattt gattgataaa
    12721 aattaatagc gagcaatcag tttgatttaa attgaattcg agaatataca tactagggca
    12781 tcaattaata aatatcaatc ttatgcaaat ttgacaattg tttgaatcaa tatataaaca
    12841 ggcaacggtt cttttcaaat atattgttaa gtgtataatg aaaatgtaaa tattattaaa
    12901 aatgggggtt cactcaatga aaatgaaacg ttttatagct attgtaatgg cattattttt
    12961 agtattagct ggttgttcta attctaatga taatcaagac aacaaaaaag atgacgcagc
    13021 caatggtaag aaacaagaaa ttcaagttgc agcggcagca agtttaacag atgtaaccaa
    13081 gaaattagct tcagaattta aaaaagagca taaaaatgct gatattaaat ttaactatgg
    13141 tggatcaggg gcattaagaa aacaaattga atcaggcgca cctgttgacg tatttatgtc
    13201 tgcaaatact aaagatgtag atgcattaaa agacaagaat aaagcacatg atacatataa
    13261 atatgcgaaa aatagtctag tattaattgg tgataaagat tcaaattata cttcagtaaa
    13321 agacttaaaa gacaatgata aattagcatt aggtgaagtg aaaactgtac cagcaggaaa
    13381 atatgcgaaa cagtatttag ataacaataa cttatttaaa gaagtcgaaa gtaaaatcgt
    13441 ttatgctaaa gatgtaaaac aagtattaaa ttatgttgaa aagggtaatg cgaaacaagg
    13501 ttttgtgtat aaaactgact tatataaaca aaataaaaaa attgatactg taaaagtaat
    13561 taaagaagta gaacttaaga agccaatcac atacgaagct ggtgctacat cagatagtaa
    13621 attagcaaaa gagtggatgg aattcttaaa atcagataaa gctaaagaaa tattaaaaga
    13681 ataccacttt gcagcataag gagttgtaat ccatgcctga cttaacacct ttttggatat
    13741 caatacgagt tgctgtaatc agtacgatta ttgtaacagt tttaggtatt tttatatcta
    13801 aatggttgta tcgtcgtaag ggttcgtggg ttaaagtatt ggaaagttta ttgatattac
    13861 ctattgtttt gccgccaacg gtattaggtt ttattctatt aatcatcttc tcgccaagag
    13921 gaccaatcgg tcaattcttt gcgaatgtac tacatttacc tgtagtgttc actttgacag
    13981 gtgctgtgat agcatctgtc attgttagtt ttccactaat gtatcaacat actgtgcaag
    14041 gcttcagagg tatagacacg aaaatgatta atacagctag aacgatggga gcaagtgaaa
    14101 cgaaaatttt cctcaaatta attttaccat tagctaaacg ctctatttta gcaggtataa
    14161 tgatgagttt tgctcgtgca ttaggtgagt ttggtgctac attaatggtt gcaggatata
    14221 ttccaaataa aaccaataca ctacctttag aaatatactt cttagtggaa caaggtagag
    14281 aaaatgaagc gtggttatgg gtattagtgc tagtcgcatt ctctattgtg gttatatcta
    14341 caattaattt attgaataaa gataaatata aggaggtcga ctagatgctt aaaatcaatt
    14401 tgaaatatca attaaagaac actttaattc gcatcaatat aaatgatacc gaaccaaaaa
    14461 tttatgcagt tcgtggtcca tctggcattg gtaaaactac tgttttaaat atgattgccg
    14521 gattacgtaa agcagatgaa gctattatcg aagtgaatgg gcaattgcta actgatacgg
    14581 caaaaaacgt gaatgttaaa attcaacaac gacgtattgg atatctgttt caagactacc
    14641 aattgtttcc taatatgacg gtctataaaa atattacttt tatggctgaa ccatctgaac
    14701 acatcgatca attaattcaa actttaaaca ttgatcattt gatgaaacaa tatcctatga
    14761 cattgtcagg tggagaggca caacgtgtag cacttgcacg tgcacttagc acaaaaccag
    14821 atttaatttt attagatgaa cctttttcta gtttggatga tactacaaaa gatgagagta
    14881 ttacattagt taaacgtatt ttcaacgaat ggcaaatacc aatcatattt gtgacacatt
    14941 caaactatga agcagaacaa atggctcatg aaattattac aattgagtaa tcatttattt
    15001 gccattaaag agtttagaac gtatttaaaa ttgtagaagt gaatgcttct atcagcattt
    15061 taatgatgtt ttaaactctt ttttaggggc agttttttga gagacattga cgcgcgtcat
    15121 ataatgaaag taatgataaa aagaaaggat aacttaatgt gagtcaagaa cgttattcaa
    15181 ggcaaatttt atttaaacaa ataggtgaaa taggtcaaag caaaataaat caaaaatgtg
    15241 cgttgattat tggtatgggc gcattaggta cacatgtggc tgaaggactt gttagagcag
    15301 gcattgctaa actaatcatt gttgatagag attatattga atttagtaat ttacaaagac
    15361 aaacattgtt tactgaagaa gatgctttga aaatgatgcc taaagtggtt gcagctaaaa
    15421 agcatttgct agcgttacgt agtgatgttg atattgatga ttatattgcc catgtggatt
    15481 attatttttt ggaaacacat ggacaggacg ttgacgttat tattgatgca accgataact
    15541 ttgaaacacg acaactgatt aatgattttg catataaaca tcgtatacct tggatttatg
    15601 gtggtgttgt acagagtaca tatacagaag ctgcatttat acctggtaaa acaccttgct
    15661 ttaactgttt ggtaccacaa ttgccagcat taaatttaac atgtgataca gtaggggtca
    15721 ttcaacctgc cgtgacgatg gcaacaagtt tacaattaag agatgcgatg aaagtattaa
    15781 cggaacaacc aattgacaca aaaataactt atggcgatat ttgggaaggt agtcattatt
    15841 catttggttt cagtaaaatg caacgttcag cctgtacaac ttgtggagat gtaccaagtt
    15901 atccgtattt aaacaaaaat gaacaacgtt atgcaacatt gtgtggtaga gacactgtac
    15961 agtatgaaaa tgcatcaatt acacacgaca ttcttgttca atttttaaaa caacatcaac
    16021 taaattatcg cagtaattcg tatatggtta tgtttgaatt taaaggacac cgcattgttg
    16081 cttttaaagg tggaaggttt ttaatacatg gcatgacacg cacatcagat gccatacatc
    16141 taatgaattt attgtttgga taaaaaaaag ataagacaaa aggagtgtaa tattatgggc
    16201 gaacatcaaa acgttaaatt gaatcgtaca gttaaagcag ccgtactaac ggtatcagat
    16261 actagagact ttgatacaga taaaggtggt caatgcgtgc gccaattgtt acaagcagat
    16321 gacgttgaag tgagtgacgc acattataca attgtgaaag atgaaaaagt agccatcacg
    16381 acgcaggtga agaagtggtt agaagaagat attgatgtca tcattacgac tggtggaacg
    16441 ggtattgcac aacgtgatgt gacgattgaa gcagtaaaac cacttttaac taaagagata
    16501 gaaggctttg gggaattgtt tagatatttg agttatgttg aagatgttgg cacgcgtgca
    16561 ttattgtctc gtgctgtagc aggtacaatt aataataaat tgatattttc gattccagga
    16621 tcaacaggcg cagttaaatt agcattagaa aagctcatta aaccagaatt aaatcatctg
    16681 attcatgagc ttacaaaata atttattgac ctgattggcg ttgaaaatct ccagatttac
    16741 cgccagactt gctttcaagg taggtttcgc caataatcat acctttatca actgctttcg
    16801 tcatgtcgta aatggttaaa gccgttgctg atgcagcggt taaagcttcc atttcaacac
    16861 cggttttgcc agttgtagag acagttgttt gaatgtttaa agtataaagg ggtgcatttg
    16921 tttcatccca gctgaagtga acatctatgc cagtcaatgg taatggatgg cacatcggaa
    16981 taattgttga tgtatttttg gcagccataa taccagcgat ttgagcagtg ttcaatacat
    17041 tgcctttggt attggtgtta ttagaaatct gcttataaat agtttcattc acagtaatgc
    17101 ttgaatgagc gacagcagtt cttttggtaa tttgtttgtc tgatacatcg accattttgg
    17161 cgtggccttg ttgattaata tgagtaaact cagtcatttt acccctccta gtgcatctag
    17221 tatatcatga aaaaataaaa gttttggaga tgatttttaa tggtagtaga aaaaagaaac
    17281 ccaatcccag ttaaagaagc aattcaacgt atcgttaatc agcagagttc aatgccggca
    17341 attacggtag cacttgaaaa aagtctaaat catatcttag cagaagatat tgtagctact
    17401 tatgatatac caaggtttga taaatcacct tatgatggtt ttgcaattcg cagtgttgat
    17461 tcacaagggg caagtggtca gaatcgcatt gagtttaaag taattgatca tattggtgca
    17521 ggttcagttt ctgataaatt agttggggat cacgaagcgg tgcgtattat gactggagca
    17581 caaataccta atggcgcaga tgctgttgtt atgtttgaac aaacgattga attagaagat
    17641 acatttacaa ttcgtaaacc attttcaaaa aatgaaaata tatctttaaa aggtgaagaa
    17701 acaacgacag gcgatgttgt tctaaaaaaa ggacaagtaa ttaatccggg ggctattgcg
    17761 gtccttgcaa catatggcta tgcagaggtt aaagttatta agcaaccaag cgtcgctgtt
    17821 attgcaacag gaagcgaatt attagatgtt aatgatgtat tagaagatgg aaaaattcgt
    17881 aactctaatg gtccaatgat tcgtgcctta gcagaaaaat taggtcttga agttggtatt
    17941 tacaaaacac aacaagatga tttagatagt ggtatacaag tcgttaaaga agctatggaa
    18001 aaacatgata tcgttattac aacgggcgga gtttctgttg gagattttga ctatttacct
    18061 gagatttata aggctgtaaa ggcggaagtg ttatttaata aagtagcaat gcgtcctggt
    18121 agcgtaacaa cggttgcatt tgcagatgga aaatatttgt ttggattatc tggaaatcca
    18181 tcagcttgtt ttacaggatt tgaactattt gtgaaaccag ctgttaaaca tatgtgtggc
    18241 gcactagaag tcttcccgca aataattaaa gcaacattaa tggaagattt taccaaggca
    18301 aacccattca cacgatttat acgtgctaaa gcaacgttaa caagtgctgg agctactgta
    18361 gtaccttcag gattcaataa atcaggtgcg gttgtagcga ttgcacatgc taactgtatg
    18421 gtcatgttac caggagggtc acgtggtttt aaagcgggtc atacagtaga tattatattg
    18481 actgaatctg acgctgcaga agaggaactt cttttatgat tttacaaatt gtagggtaca
    18541 aaaagtctgg taagacaaca ttgatgaggc atattgtctc tttcttaaag tcacatggtt
    18601 atacagttgc tactattaaa catcatgggc atggtaagga agatattcaa ttacaggatt
    18661 cagacgtcga tcacatgaag cattttgaag cgggggcaga tcaaagtatt gtacaaggat
    18721 ttcaatatca gcaaactgta acacgtgtag ataatcaaaa tcttactcaa attattgaaa
    18781 aatctgttac aattgacacc aatgtcgtat tagttgaagg ctttaaaaat gctgattttg
    18841 aaaaagtcgt agtctatcga aatgaagaag agttgcaagt attacaacag ttgtcgaatg
    18901 tttgttatag cattaatgtt agggagcatg aagattttac agcatttgag caatggttat
    18961 taaataaaat taaaaatgat tgtgatacac aattaacata gaggattgaa atgaatgaaa
    19021 caatttgaaa tcgtgacaga accgatacaa acagaacaat atcgtgaatt cactataaat
    19081 gaatatcaag gtgcagtagt tgtttttacc ggtcatgttc gcgaatggac taaaggcgtc
    19141 aaaacggaat atttagaata tgaagcgtat attacaatgg ctgaaaagaa attggcacaa
    19201 attggagatg aaataaatga aaaatggcct ggaacgataa cgagtattgt tcatagaata
    19261 gggccattac aaatttcaga tatcgctgta ttaattgcag tttcttcacc gcatcgtaaa
    19321 gatgcctatc gagcaaatga atatgcaatt gagcgtataa aagaaattgt tccgatttgg
    19381 aaaaaagaaa tttgggaaga tggttcaaaa tggcaagggc atcaaaaagg gaattatgaa
    19441 gaagcgaaga gggaggaata agagagatga aggtacttta cttcgcagaa attaaagata
    19501 tattacaaaa agcacaggaa gatattatgc ttgaacaagc attgactgta caacaatttg
    19561 aagatttatt gtttgaacgt tatccgcaaa tcaataataa aaagtttcaa gttgctgtaa
    19621 atgaggaatt tgtacaaaaa tcagatttca ttcaacctaa tgatactgtt gcattaattc
    19681 caccggttag tggaggttaa gggagcatga aagcaataat tcttgcaggt ggtcattcag
    19741 tacgatttgg taagcccaaa gcttttgcgg aagtgaacgg tgagaccttt tatagtagag
    19801 taattaagac attagaatca acaaatatgt tcaatgaaat tattattagt acaaatgcgc
    19861 aattggcaac gcaatttaaa tatccaaatg ttgttataga tgatgagaat cataatgata
    19921 aaggtccatt agcaggaatt tatacaatca tgaagcaaca tcctgaagaa gaattgtttt
    19981 ttgtcgtttc tgttgataca ccaatgatta ctggtaaagc tgtaagcacg ttgtatcagt
    20041 ttttagtttc tcatcttatt gaaaatcatt tagatgtcgc agcttttaaa gaagatggac
    20101 gttttattcc aacaattgca ttttatagtc cgaatgcatt aggcgctata actaaagcac
    20161 tacattctga taattacagt tttaaaaatg tatatcatga attatcaacg gattatttgg
    20221 atgtcaggga tgtagatgcg ccctcatatt ggtacaaaaa tataaattat cagcatgatt
    20281 tggacgcttt aattcaaaaa ttgtaagccg ttaggaggtc cacaaatggt agaacaaata
    20341 aaagataaac taggacgtcc catccgtgac ttacggttat ctgtgacaga tcggtgtaac
    20401 tttaggtgtg attattgcat gcctaaagag gtatttggag atgatttcgt atttttacct
    20461 aaaaatgaac tattaacgtt tgatgaaatg gctagaatcg ctaaggtata tgcagaatta
    20521 ggtgtaaaaa aaatacgtat tacaggtgga gaaccattga tgcgacggga tttagatcta
    20581 cttattgcta aattaaatca aatcgatggt attgaagata ttggtttgac tacaaatggt
    20641 ttgttattaa aaaagcatgg tcaaaagtta tatgatgctg ggctacgcag aattaatgtc
    20701 agtttggatg ctattgatga tacgctattt caatcaatca ataatcgtaa tattaaagcg
    20761 actacgattt tagaacaaat tgattacgcg acgtctattg gtttgaatgt aaaagtaaat
    20821 gttgttatac aaaaaggtat taacgatgat caaattatac caatgcttga atattttaaa
    20881 gataaacata tagagattcg ttttatagaa tttatggatg ttggtaatga taatggatgg
    20941 gatttcagca aagttgtaac taaagatgaa atgcttacta tgatagagca gcactttgaa
    21001 atcgatcctg tagaaccaaa atattttggg gaagtagcaa aatattatcg ccataaggat
    21061 aatggtgttc aatttggttt gattacaagt gtttcacaat cattttgttc tacatgtaca
    21121 cgcgcaaggc tgtcatcaga tgggaagttt tacggatgtt tatttgcaac tgtcgatgga
    21181 tttaacgtta aagcgtttat tcgttctggc gtgaccgacg aagaattaaa agaacaattt
    21241 aaagctttat ggcaaataag agatgatcga tattcagatg agagaactgc tcaaacagtt
    21301 gccaatcgtc aacgtaaaaa gataaacatg aattatattg gtggttaatg tgtaggaacc
    21361 actacatatt aaatcattag agattcttta atatttctgt cttactccct aaaatacaat
    21421 attatttatt aaagtaaaaa cggtcatatc tatgccagat ttaatagaaa tgatcgtttt
    21481 taatgttttt acaaatcggc ggggccccaa cacagaagct gacagaaaga cagcttacaa
    21541 taatgtgcaa gttgtcgggg ccccaacata gaaggtgacg aaaagtcagc ttacaataat
    21601 gtgcaagttg gcggggcccc aacatagaag gtgacgaaaa gtcagcttac aataatgtgc
    21661 aagttgggga acggggaccc aacatagaga atttcaaaaa gaaattctac agacaatgca
    21721 agttgtcggg gccccaacat agagaatttc aaaaagaaat tctacagaca atgcaagttg
    21781 gggaacaacg aaataaaatt ttatgaaaat atcatttcta tcccactctt aagaatcact
    21841 acataataaa tctttagtgg ttctttaaca ttgatgtcac actccatgtc attgagttgt
    21901 aatatatctt ttttaggtac aaatgttgtc gaataaacaa caagttgtcc aaaagatata
    21961 aatctaaaca agatatagcc agcaatttaa tatttgtaat agataaaatg ctaagtttga
    22021 tatataataa atttaagtaa ttgtataata atatgaatta caaacatcta agaagaaaca
    22081 taggaggcat catattatga gtaataaagt tcaacgtttt atagaagcag aaagggagtt
    22141 aagtcagtta aagcactggt taaaaacaac gcataagatt tcaattgaag aatttgtagt
    22201 actttttaaa gtgtatgaag ctgaaaagat tagcggtaaa gaattgaggg atacattaca
    22261 ttttgaaatg ctatgggata caagtaaaat cgatgtgatt atccgtaaaa tctataaaaa
    22321 agagcttatt tctaaattgc gttctgaaac ggatgaaaga caagtattct atttctatag
    22381 tacttctcaa aagaaattgt tagataaaat tactaaagaa atagaagtgt taagcgttac
    22441 aaactaaaaa cttaaaaagc atgccaatca gtacatcata attgcgtctt ggggacagac
    22501 aaatgatgaa tagagattgg catgcttttt atttttgaat ataaatattt agttcatggc
    22561 atttctagtt acatgacgtc catgaattaa gaagtaaaca agcatagtaa tgattgctaa
    22621 agcggccata aagccgaaga tttcactata tgaaaacata tgagtaaata acccaaggaa
    22681 tgatggaccg aagccgacac ctgcatctag accaacgtaa aaagtagatg tcgcgatacc
    22741 atatttaatc ggaggtgaga cttttatcgc aatagattgc attgcagatg ataaatttcc
    22801 ataccctaaa cctaggcaag ccccagcaag taatattaac cagctttgat agcttgaaat
    22861 taagcacaca aatgaaagga aaagcatgat aaatgctggg tagacaataa tattttcatt
    22921 tttatcatcc atcaatctac cagcaatagg tctagtaatt aacgatgcta tagcatagca
    22981 aataaagaaa tagcttgctg cagtgactag gtgtcgctct aaagcaaatg cttgtaaata
    23041 agttaggatg gacgcatagg taacgccaat taaaagcata attacagcaa caggaatggc
    23101 ctcttttgca ataaattgat gaatactaaa tcttggttta tcaatgacat tagtttcagt
    23161 tttgttattt gttacttcga aatcaacttt tataaataat gagataatga gtccgagtat
    23221 gcctaatatg acacaaataa taaacagtaa attaattgcg tattttgtaa taagtaacat
    23281 acctagaaat gggccaatcg ctgtacctaa tactaaactt aaggaaaata aactgatgcc
    23341 ttcacttttt ctattaacag gggtaacgta tgccgcaata gtacctgttg cagttgtcac
    23401 aactgcagtt gcgataccgt ttatgagacg tacaaagatt aaaaaagcta aagatccatc
    23461 aataaaataa attaattgcg tgataattaa agcaattaaa ccaataaata ataatcgttt
    23521 aggtccgatt tgatttacaa atttacctgt agcaaatcga ccaattaaag acccgacaat
    23581 aaatagtcct acaactaatc cggctaatga atcggaagcg tcgaaagctt gcttactata
    23641 gccagcaata actacaagta acaaatacat gcataggtat acgataaagt tgactaaaaa
    23701 gttaattgta aaacttttgg tgaaaatagg tgatttattt aatgagcctt gattaatcat
    23761 tgtgtgcctc ctttaataat atttcatgaa tttgattcat cacatttatg gtttcctgca
    23821 tttgatcttg cgaaatgttt gttttggcaa ctactgcttg ttggagcttt tgtacatcaa
    23881 gacaaacaat cccatataat tcatgccctt taggtgttaa agttaaaaac ttttgtcgtt
    23941 tatcctctaa actgttttct actgtaataa ggtcattttc tattagagct tttaaagttt
    24001 ttcttgcagt aggcttttca attgcacgtc tatgtgaaat ttcaatgaga gtagtgggtt
    24061 gatatttagc gatatcgcgt aagattaacc attgaccata atatatattg tgtttttcca
    24121 aaatcggctc tgctaatttt aaataggggc gatatattgt tataaaacta ttgaaaaatt
    24181 cttgtgatag catagtgaca tctcctaaga taaaatagtt agcttggcta acctttttca
    24241 caacaatagt aattataaaa cgggggcaat tagaaatcaa tatataatta ttaagagcaa
    24301 aaatatttat actttgttaa aataagcgta attacatgta aataggggga tactaatgat
    24361 attgaaattt gatcacatca ttcattatat agatcagtta gatcggttta gttttccagg
    24421 agatgttata aaattacatt caggtgggta tcatcataaa tatggaacat tcaataaatt
    24481 aggttatatc aatgaaaatt atattgagct actagatgta gaaaataatg aaaaattgaa
    24541 aaagatggca aaaacgatag aaggtggagt cgctttcgct actcaaattg ttcaagagaa
    24601 gtatgagcaa ggttttaaaa atatttgttt gcgtacaaat gatatagagg cagttaaaaa
    24661 taaactacaa agtgagcagg ttgaagtagt aggtccgatt caaatggaaa gagatacaca
    24721 taaagatggt aaggtaaagt ggcaattgct ttatataatg aatcaggatg atgatgatga
    24781 tgaaattaag ccaccatttt ttattcaatg ggaagaaagt gattccatgc gtattaaaaa
    24841 attgcaaaaa tattttcaaa aacaattttc aattgaaact gttattgtga aaagtaaaaa
    24901 ccgatcacaa acagtatcga attggttgaa atggtttgat atggacattg tagaagagaa
    24961 cgaccattac acagatttga ttttaaaaaa tgatgatatt tattttagaa ttgaagatgg
    25021 taaagtttca aaatatcatt cggttataat aaaagacgca caagcaactt caccatattc
    25081 aatttttatc agaggtgcta tttatcgctt tgaaccatta gtataaatat acgtaagtac
    25141 tatgagcgag aatacccata tgaataatgg caagcacaat ggaaagaatc gttaatatat
    25201 tatttaatcg tgatgactta attaaaatga aaaagattga taatataaat gtgaaaaaga
    25261 taagtataac ccgtaaacta aagtaattca cggtgagagg ttgactcaat gtcataatga
    25321 ttgcaacgat gttcataatt ataaatagac ttaaaataat tgttctcata tcaaacacct
    25381 cattgttaga ttattgacat tataacaggg ggaattgtat atgaacatta atgtggttgc
    25441 ttgaggagaa atttattcat tgaagtcaaa ttggttcatg ttagaaatga atatcgtgtt
    25501 agatgatgaa agtattttga agtataggta actagttgaa aagtattaat tgtacgataa
    25561 cattaaattt aacaggaagc atagatataa aatgattcac aattaaaatg agtaaatttg
    25621 aacttgctaa actattaatt ggagcatgga catttcaaaa ataagagttc aaatcataca
    25681 caagctctga atcgacacta taagatacaa actgtataat taaaggtatt gttaaataga
    25741 aggagatatc ataaatcatg gaaaagatgc atatcactaa tcaagaacat gacgcatttg
    25801 ttaaatctca cccaaatgga gatttattac aattaacgaa atgggcagaa acaaagaaat
    25861 taactggatg gtacgcgcga agaatcgctg taggtcgtga cggtgaaatt cagggtgttg
    25921 cgcagttact ttttaaaaaa gtacctaaat taccttatac gctatgttat atttcacgtg
    25981 gttttgttgt tgattatagt aataaagagg cgttaaatgc attgttagac agtgcaaaag
    26041 aacttgctaa agctgagaaa gcgtatgcaa ttaaaatcga tcctgatgtt gaagttgata
    26101 aaggtacaga tgctttgcaa aatttgaaag cgcttggttt taaacataaa ggatttaaag
    26161 aaggtttatc aaaagactac atccaaccac gtatgactat gattacacca attgataaaa
    26221 atgatgatga gttattaaat agttttgaac gccgaaatcg ttcaaaagtg cgcttggctt
    26281 taaagcgagg tacgacagta gaacgatctg atagagaagg tttaaaaaca tttgctgaat
    26341 taatgaaaat cactggggaa cgcgatggct tcttaacgcg tgatattagt tactttgaaa
    26401 atatttatga tgcgttgcat gaagatggag atgctgaact atttttagta aagttggatc
    26461 caaaagaaaa tatagcgaaa gtaaatcaag aattgaatga acttcatgcc gaaattgcta
    26521 aatggcagca gaagatggaa acatctgaaa agcaagctaa aaaagcgcaa aatatgatta
    26581 atgatgcgca aaataaaatt gctaaaaatg aagatttaaa acgagaccta gaagctttag
    26641 aaaaggaaca tcctgaaggt atttatcttt ctggtgcact attaatgttt gctggctcaa
    26701 aatcatatta cttatatggt gcgtcttcta atgaatttag agatttttta ccaaatcacc
    26761 atatgcagta tacgatgatg aagtatgcac gtgaacatgg tgcaacaact tacgatttcg
    26821 gtggtacaga taatgatcca gataaagact cagaacatta tggattatgg gcatttaaaa
    26881 aagtgtgggg aacatactta agtgaaaaga ttggtgaatt tgattatgta ttgaatcagc
    26941 cattgtacca attaattgag caagttaaac cgcgtttaac aaaagctaaa attaaaatat
    27001 ctcgtaaatt aaaacgaaaa tagattaacg actgaaatct gaacgctcat aagactgtca
    27061 tttgcgttca gattttttta cacaatatag aatggttgag taaaatattt ttgaatatag
    27121 tgaaagaggg ggaagtactg tgataaaaaa actattacaa ttttctttgg ggaataagtt
    27181 tgctatcttt ttaatggttg ttttagttgt cttgggcggt gtatacgcga gtgctaaatt
    27241 gaaattagaa ttactaccaa atgtacaaaa tccagttatt tcagttacaa caacaatgcc
    27301 gggtgcaacg ccacaaagta cccaagatga aataagtagt aaaattgaca atcaagtaag
    27361 atcgttggca tatgtgaaaa atgttaaaac gcaatccata caaaatgctt caattgtaac
    27421 agttgaatat gaaaataata cagatatgga taaagcagaa gaacagctta aaaaagaaat
    27481 cgataaaatt aaatttaaag atgaagttgg tcaaccagaa ttaagacgta attcgatgga
    27541 tgcttttccg gttttagcat attcattttc aaataaagag aatgacttga aaaaagtaac
    27601 gaaagtactg aatgaacaat taataccaaa attacaaacg gtagatggtg tgcaaaatgc
    27661 gcaattaaat gggcagacga accgtgaaat cacccttaaa tttaagcaaa atgaacttga
    27721 aaaatatggg ttaactgctg atgatgtaga aaactatcta aaaacagcaa caagaacaac
    27781 accacttgga ttgttccaat ttggtgataa agataaatca attgttgttg atggtcaata
    27841 tcaatctgtt gatgctttta aaaacataaa tattccatta acgttggcag gaggacaagg
    27901 gcaatctcaa tcccaaagtg acaataaaca aaattcagcc atgtcagacg ttaatcaggc
    27961 atcaccacag caaaattcaa aagcgtcagc aaataatata agtggtatgc cgacagtgaa
    28021 actaggagat ttagctgata ttacagttgg tgatgtgcgt acttctattt ctaaaacgaa
    28081 tggcaaggat gcggttaatt tacaaataac taaagctcaa gatgccaata cagttcaagt
    28141 agccaaagat gtacaacgta aaattgatac attcgttgat gaaaataaag attttaatgt
    28201 cacaaaaaca atggatactg caaagcctgt tgagaaatca ctttatacga tggttgaaaa
    28261 agcatcatta ggtacaatcg tggcaattat agttattttg ctgtttttaa gaaacattcg
    28321 tacgacggca atttctatta tatccattcc gttatcactt cttatggcgc ttattgctct
    28381 gaaattgagt gatgtttcat tgaatatact aacgttaggt gcattaacag tagcgattgg
    28441 acgtgtgata gacgattcga ttgtagttgt tgaaaatatt tatcgacgct taacagattc
    28501 agaagaacaa ctaaaaggtg aaaatttaat tatcagtgcg acaactgaag tatttaaacc
    28561 aataatgtca tcgacactag ttactattat cgtcttttta ccacttgtgt ttgtatcagg
    28621 ttcagtaggc gaaatgttta gaccttttgc attggctatt gcatttagtt tattagcatc
    28681 gttattagtg tcaattacac tcgttccagc attggcagct acactattta aaaaaggcgt
    28741 taaacgtcgt aataaacaac atcaagaagg attaggtgtt gttagtacaa cttataaaaa
    28801 agtattgcat tggtcactaa atcataagtg gattgtaatt atattaagta cattaatttt
    28861 ggttgcaact gttgtatttg gaggaccgag actaggcact agctttattt cagcaggtga
    28921 cgataaattt ttagctatta cttatacacc gaagcctggt gaaacggagc aagcagtgtt
    28981 gaatcatgcg caagatgttg aaaaatattt aaaacagaaa aagcatgtaa aaacaattca
    29041 atactcagtt ggcggtagta gtccagtaga tccaacgggt agtacaaata gtatggtaat
    29101 catggttgaa tatgataatg acacgcctaa ttttgatgta gaagcggata aggttattaa
    29161 acatgcagat ggctttaaac atcctggaga gtggaaaaat caagatttag gaacaggtgc
    29221 aggtaataaa tctgtagagg ttactgtgaa aggcccatca atggatgcca taaaatcaac
    29281 tgtaaaagat attgaacaga aaatgaaaca ggttaaagga ctagccaatg tcaaatctga
    29341 tttatcgcaa acatatgatc agtatgaaat taaagttgat caaaataaag cggcagaaaa
    29401 tggtatttct gcaagtcaac ttgcaatgca cttgaatgaa aacttaccag aaaaagcagt
    29461 tacgactgtt aaagaaaatg gtaaaactgt tgatgttaaa gtcaaacaaa ataagcaaac
    29521 agactggtca gaagataagt tgaataatat aactttgaaa aagccgactg gtgatacgat
    29581 taaattggga gatatcgcta cgttagttaa aacaacgaca ccaagtaaat tgacgcaaga
    29641 acaaggtgat tatgcaacga cggtatctgc taaagtaaca aataaagatg tgggtggcac
    29701 aacacgacaa gtgatgtcta aaataaataa tttggacaaa ccgaataatg taaaggttaa
    29761 tatcggtggt gcatcagatg atattaacaa tgcaatgaca caattagcct ttgcaatgtt
    29821 agctgcaatc attatcgtat atttaatcct agttattaca tttaaaggtg gcttagcacc
    29881 atttacaatt ttattctctt taccatttac agttatcggt gtaattattg cactattaat
    29941 cacaggagaa acaatatcag taccaagttt aattggtatg ctaatgttaa ttggaattgt
    30001 agtaacaaat gccattgtgt taatagaccg tgtcattaat aatgagcaac agggcatgga
    30061 gatgaaagaa gcattaatcg aagcaggcgg tactagaatt agaccgatac taatgacggc
    30121 gattgcaaca atcggtgcat tagttccttt gttatttggt caagatagct cgattcttat
    30181 ttcgaaagga ttagctgcta cagttattgg tggtttaatt tcatcaacgt tattaacact
    30241 tgtagttgta ccagtaatat atgaaattct ctttacttta aaaaaacgat tcactaaacg
    30301 atagataata taaaagccat aaaagcggtc atgatattga ctacttttat ggctttttaa
    30361 aatttggaat tttagacctt atttcacgtg cgatttaatc gatgtgttaa taaaacaata
    30421 ctggcattca acatgtaatc cgataataaa ataagattgc aattatgtta ttggatgttt
    30481 tgtttattac ttagaataat acgatggtta agatgaaata tatgacaaag ataaataatg
    30541 acattaaatt caagatgttt acgcctttta aatctttttt aagcatttta aatcctgtca
    30601 cttcgacatt accagctaag agtaataaga cgataaatac ccaccaattc tcttttggaa
    30661 aactcatcgt cgttaatgct aagacgaata ataacaacac acccataata attctcaaaa
    30721 tgcgagtaaa tacaacttcc attttaaaaa tactaataaa actaacaatt agataaagta
    30781 ttgctagtaa aatcagataa aattgcatat gataacttcc tctccgtttc aaagggttca
    30841 tgtagtacag cagcgtagaa aacataaata gcagagttgg gatagatatg cgtgtatgta
    30901 gttaatgaga cactaaatca taacacgaca acatcttgtg attctagatg taattgagcg
    30961 aaaaataaag caattcatca atataacgta ttcagagcaa tgctagtcaa atttatgtaa
    31021 cgaaaagcat ttattgcttg aatgatagat tattaatctt gcatttgagc gcgaacgttt
    31081 ctaatttcat tttcaatatc tttaagttta cgaacaagtg cggaagtatc taaattcaaa
    31141 gattcccgtt tttctaagtc accttgaaga cgaacataat catatttaag ttcggctagt
    31201 tggttttcta aagacatact ataacactcc tttgaaatat aattaagtgc atttaatgta
    31261 gattatagca tgaaacagta gacttctaaa atatctgtgg agtatgttaa agttataaat
    31321 tatgaaggtt attgaaagaa gggataacag tgacagaaca atttactatg attattttat
    31381 taattgcgct tggttacttt ttgaagcgca ttaattatat aaaggcaaca gatagtcaag
    31441 tcattgctac attagttctt aatgtcacac tcccatcttt agttattgtg aatttaaaca
    31501 gtgcagaatt aaaattgtca ttctccattt taccaatttt aatgattatc tatggtattg
    31561 tggcgaaaat gattgctgtc tggtttttta gaaaatatga taatcacatg cgtggatcag
    31621 taggtatgat gactggtgct atgaatattg gattgtttgc atatccacta attgaagtga
    31681 tatggcctaa aacgggactt atttattttg gtatggctga tataggtggt gcctttgtga
    31741 tgtttggaat tacttacttt gtaggtagct actttagtga aggtagtgat caattcgatt
    31801 ttaaatattt aggtaagaaa ttattacaat cagtgccact cgttacatac attgtaatgt
    31861 ttatattgaa tataagtcac atccatttac cacatgtggc aatcgatttc tttagcatac
    31921 tttcccatgc aaatatgccg ttatcaatga ttttgctagg tgtcatgttg aattttactt
    31981 tggaaagaaa gtatatacct gctactatta aatatttatg tttgcattat ggattagcat
    32041 tagttgcggg attgttagta catgtgttgt taccggtatc agatgatatg attaagacga
    32101 cattgttggt cacttggatg ttcccggttg gtgtcgcaat tatttcttat ggtattcagt
    32161 ttaaatatcg aacactgcca tttataggta tgacgaccaa tttgacaatc attattagta
    32221 tcattatttt gtacgtgtat caggcagtgt ttgtctagtt tacacatttg aaaataaaat
    32281 ccattttagt attcgtagaa aatgagagcc ccctttacca caacatgtaa atgcatattg
    32341 tgaaaagggg gcttttattt atgacatagt tgttcaactc atacattcta gcagataatg
    32401 cagtgcgtta tgcagctatc cacttcatat gtatggcgca tacttgataa atgaatatca
    32461 tcaagatttt aaaagtatcg ctaattttaa agtcttaaaa cgcatgatta atgtcatgat
    32521 gtgttattgc gttttgtttt tagagccacg tatttcctag gacatataga ggaacattca
    32581 acgtaaaatt cccgaaaatt aattttaatc attctataag tgaatggtta ttttcagaag
    32641 tgattacatt tttacgttac atgagtattt aactttctat ttcaaattac ctagaattat
    32701 agcagctatc acgataataa taatacctgc ccaaataccc gtcatctgac gacgatcttt
    32761 tctttctcct aaaatgaaaa taccgcctaa agttgaaacg ataacaagta actgtgataa
    32821 tgagaaactt gtagctacac caacttttgg ttgagaatag aacatgaaca agttaccaat
    32881 tccccaaatc acacctggca atagattaag tgctgttgat ttaagtgatg ttttatgatt
    32941 catggataag ataaagccac caattgccat accgacagat tggaagaaca atgcatcagt
    33001 tccaccaaca ccaaagatgt caccaagtac aacgaaacct acatatccaa ctgtagatac
    33061 aattaaaata cccattgctt ttttaaattc aggattatct gattgacgtt catttttagc
    33121 tttaagtgaa gtaagtgcta caccagtaac taataatatc atggcgatta aaccaaagat
    33181 aatttgagtc attgaagacc attcacctaa aaagataacg ctaaataatg ttgtaccaac
    33241 taattgcata cctgtagaaa ctggcatagt atttgataca ccgattaaac taatagattt
    33301 aagctgattc gcttgtccaa acgcccataa tgcaccagaa ataagaccga caataattac
    33361 agtaggatta ttgaattttg cgaaaccagt aataagtaaa cctaatccaa caatgagtgc
    33421 gcctagcgtt gtaccacgaa tttggttgta aggtccaccg ccgacgaaca cattaataag
    33481 aacgacactt ccccagaata aagcaggtaa aagtgcgatt aagaaatcaa gaaattgcaa
    33541 gactactcac tctcctatac tatttatgca agtgctcccc ttaattgaca cttgcatgtt
    33601 attgtaaagc attctgccct aatagtaaaa aacaactact tataattttc aatatttaca
    33661 aaaagtagaa aaacccacaa tacatgctaa tgatgtactg tggggtattt gtttggtatt
    33721 ttattcaggg attgtttgat cttttacgtt acgtgattcg aatttgaatt taatgtactc
    33781 atagttgata tggaaaccta gaccattaaa tcgttcgatg agtttattat ttttgttacg
    33841 aattttaaaa taaacttttc taatatcaaa gttatcaaaa gcatattgca atgcatacgc
    33901 aagcaactcg aaagcgatac ctttcaaacg gtaatctaca tgagaactga agtatttaat
    33961 ctcggcgatt gactgttgtg aatcaatttc aaggtataaa taacctttaa gtaaaccttc
    34021 gctaacaaat aaaaacaaat gatgatggtc gtctaaacta tcaatgatat cttgcggtga
    34081 ctgtgcctga tatttaaatg tagttgtgtg taatttgctg aaagcacgat aaaacccttt
    34141 gtgataagga atgatatttg gttggtgcat atcttcttct aatcttgtac gggcttctat
    34201 gtaatagtca gtgaagttat aacttgcttg aataactttc attaatggtt tgtattgttg
    34261 aatgccttct tcaaaagaaa agttaaagac ggcatcatca ggttggttcg aagtcattgc
    34321 tttaaacagc gtttcaaacg tttcgacaga taatacataa ttgtcagcca cgaaagggcc
    34381 tatgactttg aatttatttt tatcgtatgc aaaagacaat atgattgctt tgattgaatc
    34441 gttttcttta atcgaaaata caccaggtga ttcaatcgca tcggttatta atacatctat
    34501 ttcattgtac tgttgaggaa gtttatataa gtatgatgct gattcataat tcgaatgttc
    34561 aatgaacgat tttatttgtg taatatcata taactgaact atgctcatgt cattacctcc
    34621 gtactttttg ttacttttat tatatagtat ttcaactgaa atgaaagtta atagtgatat
    34681 taacatgtta caatacattt aacacatcat ttgcaatcat tgaatttaaa tcaaagatta
    34741 gtggaataga tgaagcacgt tcgaaataaa agaacgtatg agaaaggata atttatgaaa
    34801 tctttaatat tagctgaaaa accatcagtc gcaagagata ttgctgatgc tttacaaata
    34861 aatcaaaagc gtaatggtta ctttgaaaat aaccaatata ttgtcacgtg ggcgttaggg
    34921 catctagtga caaatgcgac acctgaacaa tacgatgaaa atttaaagga atggcgatta
    34981 gaagaccttc caattatacc taaatatatg aaaacggttg ttattggtaa aacaagcaaa
    35041 caatttaaaa cagtaaaagc gttaatttta gataataaag tgaaagatat tattattgca
    35101 acagatgctg gacgagaagg tgaactagtt gcaagattga ttttggataa agttggtaac
    35161 aaaaagccac tccgtcgatt atggattagc tcagttacta aaaaagctat tcaacaaggt
    35221 tttaaaaatt taaaagacgg tcgtcaatat aacgatttgt attatgcagc gttagcgaga
    35281 agtgaggcgg attggattgt tgggattaat gcaacgcgtg cactaacaac aaagtatgat
    35341 gcacagctat ccctgggacg tgttcagaca ccaacgattc aattagtaaa tacacgacaa
    35401 caagagatta atcagttcaa accacaacaa tactatacat tatcattaac ggtaaaaggg
    35461 tttgattttc agctagaatc aaatcagcga tataccaata aagaaacttt agaacagata
    35521 gttaataatt tgaaaaatgt cgatggtaag attaaatctg ttgctactaa acataagaag
    35581 tcgtatccgc aatcactgta caatttaaca gatttacaac aagatatgta tagacgttat
    35641 aaaattggac ctaaagaaac attgaataca cttcaaagtt tatatgagag acataaagtc
    35701 gtaacctatc caagaacaga ttcaaactat ttaacaactg atatggtaga tactatgaaa
    35761 gaacgtattc aggcgacgat ggcaacaaca tataaagacc aagcacgccc attaatgtct
    35821 aaaacatttt catcaaaaat gtcgatattt aataatcaaa aagtatctga tcaccatgca
    35881 attattccta cagaagtgag acctgtcatg tcagacttaa gtaatagaga attaaagtta
    35941 tacgatatga ttgtcgagcg ttttttagaa gctttaatgc ctccgcacga gtatgacgcg
    36001 ataactgtaa ctttagaggt tgcagggcac acatttgttt tgaaagagaa tgtaacaact
    36061 gtattaggct ttaaatctat tagacaaggt gaatctatta cagagatgca acagcctttt
    36121 tcagaaggag atgaagtgaa gatttcaaaa acaaacatta gagaacatga aacaacacct
    36181 ccagaatatt ttaatgaagg ttcgttatta aaagcgatgg agaaccctca gaactttatt
    36241 caattgaagg ataaaaaata tgcgcaaact ttaaaacaaa caggtggtat cggcacagtt
    36301 gcaacaaggg ccgacattat cgataaatta tttaatatga atgccattga atcaagagac
    36361 ggtaaaatta aagtaacgtc aaaaggtaaa caaatattag aattagcacc agaagaatta
    36421 acgtcgccac ttttaactgc acaatgggaa gaaaaattac ttttaattga acgtggtaaa
    36481 tatcaggcga aaacattcat taatgaaatg aaagatttta cgaaagatgt tgtaaatggg
    36541 attaaaaata gtgatcgtaa atataaacac gataatttaa caaccactga atgcccaacg
    36601 tgtggtaaat tcatgattaa agttaaaact aaaaatggtc agatgcttgt gtgccaagat
    36661 ccatcttgta agacgaaaaa gaatgtacag cgcaaaacaa atgcaagatg tccaaactgt
    36721 aaaaagaaat taacgttgtt tggtaaagga aaagaagcgg tatatcgttg tgtttgtgga
    36781 cattctgaaa cgcaagcaca tatggatcag cgtatgaagt ctaaatcctc tggtaaagta
    36841 tctcgtaaag aaatgaaaaa gtatatgaat aaaaatgaag gtttagacaa taatccgttt
    36901 aaagatgcat taaagaactt gaatttatag ataaaatcga acaaagttga atcagaaaaa
    36961 cgaaaagttc gcttttggta ttgtttttta ttaagaatga tattaaacta ttaaggtatt
    37021 ttaaaaaaag gagcatccat tcgtgaaaaa ctatttccag ttcgataaat atggaacaaa
    37081 ctttaaaaga gaaatcttag gcggtatcac aactttctta tctatggcct atattttagc
    37141 agttaacccg caagttttaa gtttagcagg tgttaaaggc gtatcagaag atatgaaaat
    37201 ggaccaaggt gccatttttg tagcgactgc attagcggca tttgtaggct cgctattcat
    37261 ggggctaata gctaaatatc caattgcgtt agcaccaggt atgggattaa atgcattctt
    37321 tgcatttact gttgtgttaa ccatgggtat tccttggcaa gttggtttaa caggtgtttt
    37381 attctcaggt atattctttg cgatattaac cgtcacaggg tttagggaag ttattattaa
    37441 tgcaattcct tatcaaatga agatggctgt ttcagcaggt atagggttat tcattacatt
    37501 tgtaggttta caaagttcgg gtattatagt taaaaatgaa tctacattag taacattggg
    37561 acatttaact gatccaccag tacttttagc gatattcggt atcgttatta cagttatttt
    37621 atatgcaatt aaattgccgg gttctatctt tatcggtatg atcatcacag caatcgttgg
    37681 tatgttcact ggattgattc aaatgccatc tggtattgta ggtaagattc caagtattga
    37741 accaacgttc ggtgctgcat ttgaagcgtt taaagaccca agccaattat taacgataca
    37801 gttcttaatt gttattttaa cgttcttatt tattgatttc tttgatacag ctggtacatt
    37861 agtagccgtt gctactcaag caggtattat gaaagataat aaattaccaa gagctggtag
    37921 agcattgttc tcagattctt tagctacgat tgtagggtca gtttttggaa caactacaac
    37981 aacttcttat atcgaatcaa cttcaggtgt tgctgtaggt gctagaacag gatttgctag
    38041 tattgtaaca ggttgttgtt tcctattagc gctattcttt agtccgttaa tcgctgttgt
    38101 aacaagtgca gttacgacac cagctttagt ggtggtaggt gtattaatgg ctgcaaactt
    38161 tgctgagata aattggaaaa gttttgaagt tgcagtacca gcattcatta caatcattat
    38221 gatgccttta tcatattcaa ttgcaactgg tattgcgtgt ggatttattt tctatccaat
    38281 tacaatgctt atttctaaaa aacataaaga agtacatccg ataatgtatg tattaatggt
    38341 actattcatt ctgtacttca tatttgttca tggttaaatc cagacacaaa aaacgactgc
    38401 caatgattgg tagtcgtttt tttctttgaa gtaaggataa aataagaagc gaaataacat
    38461 aaagcaatat tatgaatgtc tttaaatatc cgatataaat atacttcttt taaaattgtt
    38521 ggacgtgttt cttctatatt atataactaa ttaaaaaggt tttaagaatt attctctatg
    38581 tccatctttg tattgtgtgt aaatgacacg agcattttta ttaggtatat aggttggcgc
    38641 tgataataca actaggacaa atagaatcat taggaaaaat aaaatccaat gaattaaaat
    38701 tcctaagaag gggataatag ctaaaaaact acctactata ccaaatattg gaatgatcgc
    38761 tataggtcta atcgcatttt gtctattcac taataatata atagtaagaa ttagataaag
    38821 taatgcatta aagagtaaag gctgccatcc aaaacttaaa ataaacgttg cacctaaaaa
    38881 aggaatgcca tagaaaaact cacaaagtat taaaaagata cctataagag ctaaagataa
    38941 ttttggtcca tttttcatat tcattcacca ccgttcttat gactaattat atagaataat
    39001 tttcaatgat aataataaaa tgattcaagg tacgaattta caataaagga ggaaataaaa
    39061 aatatctcga aaatagttga actgactaag tatataaagt aaaatataaa agtatgtgtt
    39121 agacagatag aaaaattatg tgaaaagcct tgatttatcg atgtttttcg tgtaatataa
    39181 ctaacgttgg actttaagaa aagcgatgaa gcgaaaggtt actgacacac ccggccgctt
    39241 tgccatggcg ctgtgtaaga tagttttcgt ggagaagtct atcactaaat gtagacgaat
    39301 aaggagggaa aattatggca aaacaaaaaa tcagaatcag attaaaagct tatgatcacc
    39361 gcgtgattga tcaatcagca gagaagattg tagaaacagc gaaacgttct ggtgcagatg
    39421 tttctggacc aattccgtta ccaactgaga aatcagtata cacaatcatc cgtgccgtgc
    39481 ataagtataa agattcacgt gaacaattcg aacaacgtac acataaacgt ttaatcgata
    39541 ttgtaaaccc aacaccaaaa acagttgacg ctttaatggg cttaaactta ccatctggtg
    39601 tagacatcga aatcaaatta taatagacaa ttttaggagg tggactttcg atgaccaaag
    39661 gaatcttagg aagaaaaatt gggatgacac aagtattcgg agaaaacggt gaattaatcc
    39721 ctgtaacagt agtagaagct aaagaaaatg ttgtattaca aaagaaaact gtagaagttg
    39781 atggatacaa cgcaatccaa gttggatttg aagacaaaaa agcatacaaa aaagatgcaa
    39841 aatctaataa atatgctaat aaaccagctg aaggtcacgc taaaaaagct gacgcagcac
    39901 ctaagcgctt cattcgtgaa ttccgcaatg tagacgtgga tgcttacgaa gtaggtcaag
    39961 aagtctcagt agatactttt gtagctggcg acgttattga cgtaacaggc gtatcaaaag
    40021 gtaaaggttt ccaaggtgca attaaacgcc acggacaatc tcgtggacct atgtcacacg
    40081 gttctcattt ccacagagca ccaggttctg taggtatggc ttcagatgct tctagagtat
    40141 ttaaaggcca aaaaatgcca ggacgtatgg gtggaaacac tgtaactgtt caaaacttag
    40201 aagtagttca agttgacaca gaaaacaaag ttatcttagt aaaaggtaac gtacctggac
    40261 ctaaaaaagg tttagtagaa atcagaactt caattaaaaa aggtaataaa taataagaag
    40321 tgaaaggagg aaatgcataa tggctaatta tgatgtttta aaattagacg gaactaaatc
    40381 aggttcaatc gaattaagcg atgcagtatt cggtattgag ccaaataata gcgttttatt
    40441 cgaagctatt aatttacaac gtgcttcatt acgtcaaggt acgcatgctg ttaagaatcg
    40501 ttcagcagta agcggtggcg gacgtaaacc atggaagcaa aaaggaacag gtcgtgctcg
    40561 tcaaggtaca atccgtgctc cacaatggcg tggcggtggt atcgtattcg gaccaactcc
    40621 aagaagttat gcatacaaaa tgcctaagaa aatgcgtcgt ttagctttac gctcagcatt
    40681 atctttcaaa gctcaagaga atggcttaac tgtagttgac gcattcaact tcgaagctcc
    40741 aaaaactaaa gaattcaaaa atgtattatc tacattagaa caacctaaaa aagtattagt
    40801 agttactgaa aacgaagatg taaatgttga attatcagca cgcaacatcc ctggcgttca
    40861 agtgacaact gctcaaggtt taaatgtttt agatatcact aatgctgaca gcttagtaat
    40921 tactgaagct gctgctaaaa aagttgagga ggtgctcgga taatggaagc aagagatatt
    40981 cttaagcgcc ccgtaatcac tgagaaatct tctgaagcaa tggctgaaga caaatacact
    41041 ttcgacgttg atactcgtgt taacaaaaca caagtaaaaa tggcagttga agaaatcttc
    41101 aacgtaaaag ttgcaagtgt taatatcatg aattacaaac ctaagaaaaa acgtatgggc
    41161 cgttaccaag gctatacaaa caaaagaaga aaagcgattg taactcttaa agaaggatca
    41221 atcgacttat ttaactaata aaccaattac cattaaggag gtaagcgaca atggctatta
    41281 aaaagtataa gccaataaca aatggtcgtc gtaatatgac ttcgttagat ttcgcagaaa
    41341 tcacgaaaac tacacctgaa aagtcattat taaaaccgct accgaaaaaa gcgggacgta
    41401 acaaccaagg taaattgact gtaagacacc atggtggtgg acacaaacgt caataccgtg
    41461 ttatcgattt caaacgtaac aaagatggta tcaatgcaaa agttgattct attcaatatg
    41521 atccaaaccg ctcagcaaac atcgctttag ttgtatatgc agacggtgaa aaacgatata
    41581 tcattgctcc taaaggatta gaagtaggtc aaatcgttga aagtggtgct gaagctgaca
    41641 tcaaagttgg taacgcatta ccattacaaa acattccagt tggtacagta gtacacaaca
    41701 tcgagcttaa acctggtaaa ggtggacaaa tcgctcgttc agctggtgca agtgctcaag
    41761 tacttggtaa agaaggtaaa tacgtattaa tcagattaag atctggtgaa gttcgtatga
    41821 tcttatctac ttgccgtgct acaatcggtc aagttggtaa cctacaacac gaattagtaa
    41881 acgttggtaa agccggacgt tcaagatgga aaggtatccg tccaacagtt cgtggttctg
    41941 taatgaaccc taacgatcac ccacacggtg gtggtgaagg tcgtgctcct atcggtagac
    42001 catctccaat gtcaccatgg ggtaaaccta cgcttggtaa gaaaactcgt cgtggtaaaa
    42061 aatcatcaga caaacttatc gttcgtggac gtaagaaaaa ataatataat caacttattt
    42121 gggtgtgcgg cttaaagctg cacgcacata ataagaaggg aggcgcccaa atggctcgta
    42181 gtattaaaaa aggacctttc gtcgatgagc atttaatgaa aaaagttgaa gctcaagaag
    42241 gaagcgaaaa gaaacaagta atcaaaacat ggtcacgtcg ttctacaatt ttccctaatt
    42301 tcatcggaca tacttttgca gtatacgacg gacgtaaaca cgtacctgta tatgtaactg
    42361 aagatatggt aggtcataaa ttaggtgagt ttgctcctac tcgtacattc aaaggacacg
    42421 ttgcagacga caagaaaaca agaagataat atctattaag tagaggagga catcctaatg
    42481 gaagcaaaag cggttgctag aacaataaga atcgcacctc gtaaagtaag actagttctt
    42541 gacttaatca gaggtaaaaa tgctgctgaa gctattgcaa ttttaaaatt aacaaacaaa
    42601 gcttcatcac cagtaattga aaaagtatta atgtccgctt tagctaatgc tgaacataac
    42661 tatgacatga acacagatga attagtagtt aaagaagcat atgctaacga aggaccaaca
    42721 ttaaaacgtt tccgtccacg tgcgcaaggt cgtgcaagtg cgattaacaa acgtacaagc
    42781 cacattacaa tcgtcgtaag tgacggtaaa gaagaagcta aagaagctta attaactttt
    42841 aaggagggaa tactgtgggt caaaaaatta atccaatcgg acttcgtgtt ggtattatcc
    42901 gtgattggga agctaaatgg tatgctgaaa aagacttcgc ttcactttta cacgaagatt
    42961 taaaaatccg taaatttatt gataatgaat taaaagaagc atcagtttct cacgtagaga
    43021 ttgaacgtgc tgcaaaccgt atcaacattg caattcatac tggtaaacct ggtatggtaa
    43081 ttggtaaagg cggttcagaa atcgaaaaat tacgcaacaa attaaatgcg ttaactgata
    43141 aaaaagtaca catcaacgta attgaaatca aaaaagttga tcttgacgct cgtttagtag
    43201 ctgaaaacat cgcacgtcaa ttagaaaacc gtgcttcatt ccgtcgtgta caaaaacaag
    43261 caatcactag agctatgaaa cttggtgcta aaggtatcaa aactcaagta tctggtcgtt
    43321 taggcggagc tgacatcgct cgtgctgaac aatattcaga aggaactgtt ccacttcata
    43381 cgttacgtgc tgacatcgat tatgcacacg ctgaagctga cactacttac ggtaaattag
    43441 gcgttaaagt atggatctat cgtggagaag ttcttcctac taagaacact agtggaggag
    43501 gaaaataata atgttactac caaaacgtgt aaaatatcgt cgtcaacatc gtcctaaaac
    43561 aactggtcgt tctaaaggcg gtaactacgt aacatttggt gagtttggtt tacaagctac
    43621 aacaacgtct tggatcacat ctcgtcaaat cgaatctgct cgtatagcaa tgacacgtta
    43681 catgaaacgt ggcgggaaag tttggattaa aatcttccca catacaccat atactaaaaa
    43741 acctttagaa gtacgtatgg gtgctggtaa aggtgcggtt gaaggctgga tcgcagttgt
    43801 aaaaccaggt agaattttat tcgaagttgc tggcgtttct gaagaagttg cgcgtgaagc
    43861 actacgttta gcaagtcaca aacttccagt aaaaactaag tttgtaaaac gtgaggaatt
    43921 gggtggtgaa acaaatgaaa gctaaggaaa ttagagactt aaccacttca gaaatcgaag
    43981 aacaaatcaa atcttcaaaa gaagagcttt ttaacctacg ctttcagtta gctacaggtc
    44041 aattagaaga aactgcacgt attcgtacag taagaaaaac gattgcacgt ctaaaaactg
    44101 ttgctcgtga aagagaaatt gaacaaagta aggctaatca ataattaagt aagaggaggt
    44161 tacaaaagtg agcgaaagaa acgatcgtaa agtttatgta ggtaaagttg tttcagacaa
    44221 aatggacaag actattacag tacttgttga aacttacaaa acacacaaat tatacggtaa
    44281 acgagtaaaa tactctaaaa aatacaaaac tcatgatgaa aacaattcag ctaaattagg
    44341 agacattgtt aaaattcaag aaactcgtcc tttatcagca acaaaacgtt ttcgtttagt
    44401 agagattgtt gaagagtcag taattattta atacaagttt agagataagg gaggtttaac
    44461 taatgatcca acaagaaaca cgcttgaaag tagcagacaa ctctggtgct cgtgaagttc
    44521 ttacaatcaa agtattaggt ggatctggtc gtaaaacagc aaacatcggc gatgttatcg
    44581 tatgtactgt taaaaatgca acaccaggtg gcgttgttaa aaaaggtgac gttgtcaaag
    44641 ctgtaatcgt acgtactaag tcaggtgttc gtcgtaatga cggttcatat atcaaatttg
    44701 atgaaaatgc atgtgttatc atccgtgatg acaaaggccc acgtggtact cgtatcttcg
    44761 gacctgttgc tcgtgaatta cgtgaaggta acttcatgaa aatcgtatca ttagcaccag
    44821 aagtacttta attaataaca accaatacaa ataaggaggt gcccacatgc atatcaaaaa
    44881 aggtgacaac gttaaagtta tcgcaggtaa agacaaaggt aaagaaggta aagtaatcgc
    44941 tactctacct aaaaaagacc gtgtcgttgt ggaaggtgtt aacattatga aaaaacacca
    45001 aaaaccaact caattaaatc ctgaaggtgg aatcttagaa acagaggcag caatccatgt
    45061 ttctaatgta caattattgg accctaaaac aaacgaacca actcgtgtag gttacaaatt
    45121 tgttgatggt aaaaaagttc gtatcgctaa aaaatctggc gaagaaatta aatctaataa
    45181 ttaataacaa gttgaaagga ggatcccact ttgaaccgtt taaaagaaaa gtttaacact
    45241 gaagttactg aaaacttaat gaaaaaattc aattatagtt cagtaatgga agtaccaaaa
    45301 atagataaaa tcgttgtgaa catgggtgta ggtgacgcag tacaaaattc taaagtatta
    45361 gacaatgctg ttgaagaatt agaattgatc actggtcaaa aaccattagt aactaaagct
    45421 aaaaaatcaa tcgcgacttt ccgtttacgt gaaggtatgc caatcggtgc gaaagtaaca
    45481 cttcgcggtg aaagaatgta tgaattctta gacaaattaa tttcagtatc attaccacgt
    45541 gtacgtgact tccaaggtgt ttctaaaaaa gcatttgacg gacgcggtaa ctacacttta
    45601 ggtgttaaag aacaattaat tttcccagaa atcgactatg ataaagtaag taaagttaga
    45661 ggaatggata ttgttatcgt aacgactgct aacactgatg aagaagctcg tgaattgtta
    45721 gctaacttcg gtatgccatt ccgtaaataa ttatttaaag gaggctaatt aagtggctaa
    45781 aacttcaatg gttgctaagc aacaaaaaaa acaaaaatat gcagttcgtg aatacactcg
    45841 ttgtgaacgt tgtggccgtc cacattctgt atatcgtaaa tttaaattat gccgtatttg
    45901 tttccgtgaa ttagcttaca aaggccaaat ccctggcgtt cgtaaagcta gctggtaata
    45961 aaaaagagtc tgaaaggagg caacaatcaa tgacaatgac agatccaatc gcagatatgc
    46021 ttactcgtgt aagaaacgca aacatggtgc gtcacgagaa gttagaatta cctgcatcaa
    46081 atattaaaaa agaaattgct gaaatcttaa agagtgaagg tttcattaaa aatgttgaat
    46141 acgtagaaga tgataaacaa ggtgtacttc gtttattctt aaaatatggt caaaacgatg
    46201 agcgtgttat cacaggatta aaacgtattt caaaaccagg tttacgtgtt tatgcaaaag
    46261 ctagcgaaat gcctaaagta ttaaatggtt taggtattgc attagtatca acttctgaag
    46321 gtgtaatcac tgacaaagaa gcaagaaaac gtaatgttgg cggagaaatt atcgcatacg
    46381 tttggtaata aaaaataagg aggtgccata acatgagtcg tgttggtaag aaaattattg
    46441 acatccctag tgacgtaaca gtaacttttg atggaaatca tgtaactgtt aaaggtccta
    46501 aaggtgaatt atcaagaact ttaaatgaaa gaatgacatt caaacaagaa gaaaacacaa
    46561 ttgaagttgt aagaccatct gattctaaag aagatagaac aaaccatggt acaactcgtg
    46621 ctttattaaa caatatggta caaggtgttt ctcaaggata cgtaaaagta cttgaacttg
    46681 ttggtgtagg ttaccgtgct caaatgcaag gtaaagactt aatccttaac gttggttatt
    46741 ctcacccagt agaaattaaa gctgaagaaa acattacttt ctcagttgag aaaaacacag
    46801 tcgttaaagt tgaaggtatt tcaaaagaac aagttggagc attagcatct aacatccgtt
    46861 cagtaagacc tccagagcct tacaaaggta aaggtattcg ttaccaaggt gaatacgttc
    46921 gccgtaaaga aggtaaaact ggtaaataat agataaactc taaagaaagg agtattgaaa
    46981 tgatcagtaa aattgataaa aataaagtgc gtttaaaaag acatgctcgt gttcgtacta
    47041 acttatcagg tacagctgaa aagccacgtt taaacgtata tcgttcaaac aagcatatct
    47101 acgctcaaat tattgatgat aataaaggcg taacattagc tcaagcttct tcaaaagaca
    47161 gcgacattgc tactacagca actaaagttg aattagcaac taaagtcggt gaagcaattg
    47221 ctaaaaaagc tgctgacaaa ggcattaaag aaatcgtatt tgaccgtgga ggatatttat
    47281 atcacggacg tgttaaagca ttagctgaag cagcaagaga aagcggatta gaattttaat
    47341 ttaaaggagg gacaaataca tggctcgtag agaagaagag acgaaagaat ttgaagaacg
    47401 cgttgttaca atcaaccgtg tagcaaaagt tgtaaaaggt ggtcgtcgtt tccgtttcac
    47461 tgcattagtt gtagttggag acaaaaatgg tcgtgtaggt ttcggtactg gtaaagctca
    47521 agaggtacca gaagcaatca aaaaagctgt tgaagcagct aaaaaagatt tagtagttgt
    47581 tccacgtgtt gaaggtacaa ctccacacac aattactggc cgttacggtt caggaagcgt
    47641 atttatgaaa ccggctgcac ctggtacagg agttatcgct ggtggtcctg ttcgtgccgt
    47701 acttgaatta gcaggtatca ctgatatctt aagtaaatca ttaggatcaa acacaccaat
    47761 caacatggtt cgtgctacaa tcgatggttt acaaaacctt aaaaatgctg aagatgttgc
    47821 gaaattacgt ggcaaaacag tagaagaatt atacaattaa ggagggaaaa ctagttatgg
    47881 ctaaattaca aattaccctc actcgtagtg ttattggtcg tcctgaaaca caacgtaaaa
    47941 ctgttgaagc tttaggtctt aaaaagacta acagttcagt agttgttgaa gataaccctg
    48001 ctattcgtgg gcaaatcaac aaagttaagc acttagtaac agtagaagaa aaataatcaa
    48061 ggaggtgccg aaatgaaatt acatgagtta aaaccggcag aaggttcacg taaagaacgc
    48121 aatcgtgttg gacgtggtgt tgcgacaggt aacggtaaaa caagtggtcg cggacacaaa
    48181 ggtcaaaaag ctcgttcagg cggtggtgta agaccaggat tcgaaggtgg tcaattacca
    48241 ttattccgtc gtttaccaaa acgtggtttt actaacataa atcgtaaaga atatgctatt
    48301 gttaacttag accaacttaa taaatttgaa gatggtactg aagtaactcc agctttatta
    48361 gtagaatctg gtgttgttaa gaatgaaaaa tctggtatca aaatactagg taatggttca
    48421 cttgataaga aattgacagt gaaagctcat aaattctcag cttcagcagc agaagctatt
    48481 gatgctaaag gtggagcaca cgaggtgatc taatgattca aacccttgtg aacttcttta
    48541 gaacaaaaga ggttcgtaac aagattttct tcacactagc aatgttagta atttttaaaa
    48601 tagggactta tataccagct ccaggagtta atcctgcagc ttttgataat ccccaaggtt
    48661 ctcaaggtgc cactgagtta ttaaatactt ttggtggcgg agccttgaaa cgattttcta
    48721 tttttgcaat gggtattgtg ccctacatca ctgcatccat cgtaatgcaa ttattacaaa
    48781 tggatattgt tcctaaattc tcagaatggg caaaacaagg tgaagtaggt agaagaaagt
    48841 taaataacgt tactcgttat ttagcaattt ctttagcatt tatccaatct ataggtatgg
    48901 cattccaatt taataattat ctcaaaggtg cgctgattat caatcagtca attatgagtt
    48961 atttattaat agcactagtt ttgacagcag gaactgcttt cttaatatgg cttggtgatc
    49021 aaatcactca gttcggtgtt ggtaatggta tttctattat catatttgcg ggtattttat
    49081 caacgttgcc agccagtcta attcaatttg gacaaactgc gttcgttggg caagaagata
    49141 catcattagc atggttgaaa gtattaggac ttttagtgtc actgatatta ttaactgtag
    49201 gtgctattta tgtacttgaa gccgttcgta aaattccgat tcaatatgct aagaaacaaa
    49261 ctgctcaaag attaggttca caggcaactt atctaccttt aaaagtgaac tcagccggtg
    49321 ttattcctgt aatctttgca atggcgttct tcttactgcc tagaacatta acgttgttct
    49381 atccagataa agaatgggca caaaacattg cgaatgctgc aaatccttca agtaatgttg
    49441 gtatggttgt atatattgta ctaattatac tattcacata cttctatgca tttgtacaag
    49501 ttaatccgga aaaaatggct gataacctta agaaacaagg tagctatgtt ccaggtatta
    49561 gacctggtga acaaaccaaa aagtatatta ctaaagtttt atatcgttta acttttgtag
    49621 gttctatctt cttagccgtt atttcaattc taccgatatt agcaactaag tttatgggat
    49681 taccacaatc aattcagatt ggtggtacaa gtttacttat cgttattggt gtagcgattg
    49741 aaacaatgaa atctttagaa gctcaagtat ctcaaaaaga atataaaggc tttggtggta
    49801 gataatttta ggagggcact tatgaatatc attttgatgg gtttacctgg cgcaggtaaa
    49861 ggaactcaag caagtgaaat tgtcaagaaa ttcccaatac cccacatttc aactggtgac
    49921 atgttcagaa aagctataaa agaagaaact gaattaggta aagaagctaa gtcttatatg
    49981 gaccgtggcg aattagttcc tgatgaagtg actgtaggta tcgttaagga aagaatttct
    50041 gaagacgatg caaaaaaagg ctttttatta gatggcttcc caagaacaat cgagcaagct
    50101 gaggcattaa ataatattat gtctgagctt gacagaaaca ttgatgctgt catcaatatc
    50161 gaagttccgg aagaagaatt aatgaaccgt cttacaggtc gtcgaatctg tgagtcatgt
    50221 ggtacaacgt atcatcttgt atttaatcct ccgaaggtcg aaggtatttg tgatatcgat
    50281 ggtggtaaat tgtatcaacg agaagatgat aatcctgaaa cggtagctaa tcgtttgagt
    50341 gttaatatta aacaatctaa acctatttta gatttctatg atcaaaaagg tgtattgaaa
    50401 aatattgatg gttcaaaaga tattagcgac gttaccaaag atgtcattga tattttagat
    50461 catttgtaat agatcaactt ttagcggtct attttaagtg aggcatgatt taatcatatt
    50521 agatagatga gagtggcaat taacgataat taattcccgt aatttttgtt taacttataa
    50581 aattaatcac tgatagattc gtaacaataa agttgtctat tgacgattac cttactattg
    50641 caaaaagggg gaagttaatc aatggctaaa caagatgtaa ttgaattaga aggtactgta
    50701 ttagatactt taccgaacgc aatgtttaaa gtagaattag aaaatggtca tgagatttta
    50761 gctcacgtaa gtggtaaaat cagaatgaat tacattcgta ttctacctgg cgacaaagta
    50821 actgttgaga tgtctccgta cgatttaaca cgcggaagaa ttacttatcg ttataaataa
    50881 tcgtcactcc ataatatagg gaggtataaa aatgaaagta agaccatcag taaaacctat
    50941 ttgcgaaaaa tgtaaagtca ttaaacgtaa aggtaaagta atggtaattt gtgaaaatcc
    51001 aaaacacaaa caaagacaag gttaataaaa gagaggtgta aattaatatg gcacgtattg
    51061 caggagtaga tattccacgt gaaaaacgcg tagttatctc attaacttat atatacggta
    51121 tcggtacgtc aactgctcaa aaaattcttg aagaagctaa cgtatcagct gatactcgtg
    51181 tgaaagattt aactgatgac gaattaggtc gcatccgtga agttgtagac ggttataaag
    51241 tcgaaggtga cttacgtcgt gaaactaact taaatatcaa acgtttaatg gaaatttcat
    51301 cataccgtgg tatccgtcac cgtcgtggtt taccagttcg tggtcaaaaa acgaaaaaca
    51361 acgcgcgtac tcgtaaagga ccagttaaaa cggtagctaa caagaaaaaa taataggtaa
    51421 aggaggcaaa ttttaaatgg cacgtaaaca agtatctcgt aaacgtagag tgaaaaagaa
    51481 tattgaaaat ggtgtagcac acatccgttc aacattcaac aacactattg taactatcac
    51541 tgatgagttc ggtaatgctt tatcatggtc atcagctggt gcattaggat tcaaaggatc
    51601 taaaaaatca acaccatttg cagcacaaat ggcttctgaa actgcatcta aatcagctat
    51661 ggagcatggt ttaaaaacag ttgaagtaac agttaaagga cctggtccag gtcgtgaatc
    51721 agctattcgt gcattacaat ctgcaggttt agaagtaact gcgatcagag acgttactcc
    51781 agtacctcat aacggttgtc gtccaccaaa acgtcgtcgt gtataattta tgatggtatt
    51841 gttacaggtc actgagcaaa cattttaaat taagtcgacg tatataagga ggatatttaa
    51901 atgatagaaa tcgaaaaacc tagaattgag acaattgaaa ttagtgaaga tgctaaattc
    51961 ggtaagttcg ttgttgaacc actagaacgt ggctacggta ctacactagg aaactcctta
    52021 cgtcgtatcc tactatcttc attaccaggt gcagccgtta agtatattga aattgaggga
    52081 gttttacatg aattctcagc agtagacaat gtagttgaag atgtttctac aattattatg
    52141 aacattaaac aattagcatt gaaaatttac tctgaagaag ataaaacttt agaaattgat
    52201 gtacgtgatg aaggcgaagt aacagcaagc gacattacac atgatagtga tgttgaaatt
    52261 ttaaacccag agcttaaaat tgcaacagta tctaaaggtg gtcacttaaa aattcgtcta
    52321 gttgctaaca agggtagagg ttacgcatta gcagaacaaa ataatactag tgatttacca
    52381 attggtgtaa tccctgttga ttcattgtat tcacctgttg aacgtgtgaa ctatactgtt
    52441 gaaaatacac gtgtaggtca aagcagtgat tttgataaat taacattgga tgtttggact
    52501 aatggttcaa tcacaccaca agaatcagtt tcattagcag caaaaataat gactgaacac
    52561 ttgaatatct tcgttggtct tactgatgaa gcgcaaaacg ctgaaatcat gattgaaaaa
    52621 gaagaagatc aaaaagaaaa agtattagaa atgtctattg aagaattaga cttatctgta
    52681 cgttcatata actgcttaaa acgcgcagga atcaattctg ttcaagagtt agctgacaaa
    52741 tctgaagctg acatgatgaa agtgcgtaat ttaggtcgta aatctttaga agaagttaaa
    52801 tacaaattag aagatttagg attaggatta agaaaagaag attgataaag gaggttaact
    52861 catgggttac agaaaattag gtcgtacttc tgatcaacgt aaagctatgt tacgtgactt
    52921 agctacatca cttattatta gtgaacgtat tgaaactaca gaagctcgtg caaaagaagt
    52981 tcgcagtgtt gttgagaaat taatcacttt aggtaaaaaa ggagatttag cttctcgtcg
    53041 taatgcagct aaaactttac gtaatgttga aatcttaaac gaagatgaaa ctacacaaac
    53101 tgcacttcaa aaattatttg gtgaaatcgc agagcgttac acagaacgtc aaggtggtta
    53161 cactcgtatc cttaaacaag gccctcgtcg cggtgacggt gctgaatcag taattatcga
    53221 attagtataa gaatttatat aaatacacta actactttta tataaagcaa atgagtgcaa
    53281 cgataatgtt tgccacatac aaatattgtc tagctcagag taccccatca actaaataaa
    53341 gatttaaagc gtgaactcaa caaacttatg atggggtgcg cgctttttta ttgaataaga
    53401 tatcggactt atgaacagaa atgtttttaa gtccgatttc tttatatcta cttttttgag
    53461 tgcgaatcta aaaatatgat caagctagtt tgatcaattt ctctagacct gaatgaagtg
    53521 aaagtattat cgttgaatca atcgcggcct accctatatt agtaaatcat ttgttttatt
    53581 aacgtttata cataattgtt agagaggaat aaatgtcctt atcattagtt gttaaacaac
    53641 aatttaaaga ctagaaatga gttagaaaat actagtaata tatattaaac ccagtcaatg
    53701 tcatatacag cgatttataa atattgtaaa atatgataga tacacgaatc gtaaaggaga
    53761 gagacaagtg gaggataaga attcagttat tgtatttaaa aatgtttcat ttcaatatca
    53821 aagtgatgca tccttcacat tgaaagatgt ttcttttaat atacctaaag gtcagtggac
    53881 atctattgtt ggtcataacg gttctggaaa atctacaatt gccaagttaa tgattggcat
    53941 agagaaagtt aaatctggag aaatttttta taataatcaa actataactg atgataattt
    54001 tgaaaagtta agaaaagaca taggaattgt atttcagaat ccggataatc aatttgttgg
    54061 ttcaattgta aaatacgatg tggcatttgg actcgaaaat catgcggttc cacatgacga
    54121 aatgcataga agagtcagcg aagcacttaa acaagttgat atgttagaac gtgcagatta
    54181 tgaacctaat gcattatcag gaggacaaaa gcaacgtgtg gctatagcaa gtgtattagc
    54241 acttaacccc tctgtcatta tattagatga ggcgacttct atgttagatc ctgatgcacg
    54301 tcaaaattta ttggatttag tgagaaaagt taaatcagaa cataatatta caatcatttc
    54361 tattacgcat gatttatctg aggcgatgga agcagatcat gttatagtta tgaataaagg
    54421 gactgtctat aaagaaggca cagcgactga aattttcgac catgcagaag agttaacaac
    54481 aataggtcta gatttaccat tcccaatcaa aataaatcaa atgctgggac accaaacatc
    54541 attcttaact tatgaagggc tggtggatca actatgacta tacgctttgt caatgtaagt
    54601 tatacctatc aaaaagggac accataccag catcaagcta ttcatgatgt taatacagaa
    54661 tttgaacaag gtaaatatta cgccatcgtt ggacaaacgg gtagtggtaa atcaacattg
    54721 atacaaaata ttaatgcact gttaaagccg actactggga cagttacagt tgatgacatt
    54781 actattacac ataagaccaa agataaatat attagacctg taagaaaaag aattggaatg
    54841 gtatttcaat tccccgaatc tcaattattt gaggacacag tagagcgtga aatgatattt
    54901 ggacctaaaa actttaaaat gaatttagat gaagccaaaa actatgccca tcgtctgttg
    54961 aaggatttag gcttttcaag agatgtaatg tcacaatcac catttcaaat gtcaggtgga
    55021 caaatgcgta aaatagcgat tgtatcgata ttggcaatga atcctgatat tatcgtggtt
    55081 gatgaaccta cagcaggact tgatccgcaa agtaaacgac aagtaatgcg attactcaag
    55141 tcactacaaa cagatgaaaa taagacaatt atcctaattt cacatgatat gaatgaagtc
    55201 gcgcgttatg cagatgaagt catcgttatg aaagaaggta gtatcgtgtc gcaaacatca
    55261 ccaaaagagc tcttcaaaga taaagaaaaa ttggcggatt ggcatattgg tttgccagaa
    55321 attgttcagt tacaatatga ctttgaacaa aaatatcaaa caaaattaaa agatattgcc
    55381 ttaactgaag aagcatttgt aagcttgtat aaggagtggc aacatgaaaa ataaattgat
    55441 tatagggcga tatttaccaa ttaattcttt tgttcatcat cttgatccaa gagcaaagtt
    55501 aatgtttgtt tttcttttta ttatattaat atttttctgt cattcaccat taacatatct
    55561 ttgggtgttt gcacttatct tattctttat gagattagca aaaattcaat tatggttcct
    55621 aatcaaaggt ttaacaccaa tatttttctt tttaatcttc accttaatga tgcatatatt
    55681 tttaactaaa ggtggatatg tattagttga atggcatggt atcacgattg aaacaaatgg
    55741 tattttagaa gggctatata tatcactgcg tttaattggg attgtgatga ttgcaacaat
    55801 tatgacacta tctacaagtc caattgattt aacagatgcg tttgaaagat tacttgcgcc
    55861 actaaaaatg tttaaattac cagttcatca attaagtatg ataatgtcca ttgcgttacg
    55921 attcatcccg acgttaatgg atgagttaga taaaatcatt ttggcgcaaa agtcgcgtgg
    55981 ttctgaaata agttcaggga acattgcaac acgtattaaa tcatttattc cattactagt
    56041 gccactattc atctcagctt tccaacgcgc cgaagaatta gcggtcgcaa tggaagttag
    56101 gggttatgat gccaatgtta agagaacgag ctacagacag cttaaatggc aattgagaga
    56161 tacgttatct ttaatcatga taatcccaat tgcaattatt ttattcgtat taaaatattc
    56221 aggagtgtaa attcatgcgt atattagtag aaattgcgta tcaaggaaat aattttctag
    56281 gttttcaaat tcaacaaaat ggtcgaacgg tacagcaaca atttgaaaag ctattacaac
    56341 gcatgcataa aagacatgta agaatccatc cttcaagtag gactgataga ggtgtacatg
    56401 cgatacagca atactttcat ttcgacacag agctgaatat acctatgtca caatggcaat
    56461 atgcaatgaa ccgaacgtta cctgatgata tttatgtcaa taatgtagtt acagtcgatg
    56521 acgattttca ttgtcgttac gattgtgtcg gtaaacgcta tcgatataaa gtatatcaag
    56581 cacaacaccg cgatccattt caaagtggtt tgaaaacatt tattcctgaa acgttggatt
    56641 tagataaaat gaacagagcg gcacaacaat tcaaaggtac acatgatttt acgggatttt
    56701 gttcgcaaaa aactgaagta gaaagcaaag ttagaacatt ataccaaagt gaaattgtaa
    56761 agacagatga tggctttgat tatattgtca ctggatctgg atttttatat aacatggtgc
    56821 gagtcttact tgcattttta atagaagtcg gaaaagggcg acatgaagta tctgatgtac
    56881 cgaagctact tgaatcaaag aatcgtaaga atgttccttt cactgcaccg gctgaaggac
    56941 tatatttaga gaagatttat ttagatgaaa atgagttatt aaaagacttt ggcaacgata
    57001 taaaaataca tcgcaaaaaa tcattacaaa atgactgatt gccattgaca aaaagcttgt
    57061 gaaattataa gattattaac ggtattgttt tatatccacc ccacgataag ccccggaaac
    57121 ttattgtgtt acaagatata taagcagaaa cgaacaacag ttaacaaaat aaatgaaatt
    57181 aaacgtttta aaaatgaaac aaatgaaatc atctattagg ttatgaaact gtttatagct
    57241 tgaatagaag catttatttt ttaggaggac aattattatg cgtcaaacat ttatggcaaa
    57301 tgaatcaaac attgagcgca aatggtatgt tatcgatgct gaaggccaaa cattaggtcg
    57361 tttatcatca gaagtagcat ctatcttacg cggtaaaaat aaagtaactt acacaccaca
    57421 cgttgatact ggtgattatg taatcgttat taatgcatca aaaatcgaat ttactggtaa
    57481 caaagaaact gacaaagttt actaccgtca ctcaaatcac ccaggtggta ttaaatcaat
    57541 cactgctggt gaattaagaa gaactaaccc agaacgttta attgaaaact caattaaagg
    57601 tatgttacca agcactcgtt taggcgaaaa acaaggtaaa aaattatttg tatatggtgg
    57661 cgctgaacat ccacacgctg cacaacaacc agaaaactac gaattacgtg gttaattaga
    57721 aggaggaaat gactttggca caagttgaat atagaggcac aggccgtcgt aaaaactcag
    57781 tagcacgtgt acgtttagta ccaggtgaag gtaacatcac agttaataac cgtgacgtac
    57841 gcgaatactt accattcgaa tcattaattt tagacttaaa ccaaccattt gatgtaactg
    57901 aaactaaagg taactatgat gttttagtta acgttcatgg tggtggtttc actggacaag
    57961 ctcaagctat ccgtcacgga atcgctcgtg cattattaga agcagatcct gaatacagag
    58021 gctctttaaa acgcgctgga ttacttactc gtgacccacg tatgaaagaa cgtaaaaaac
    58081 caggtcttaa agcagctcgt cgttcacctc aattctcaaa acgttaattg tcggacgata
    58141 tatacaaaac acctcgatgt tatgtcgagg tgtttttttg cgtttgtgta ttaaattatt
    58201 caaagttttg gggatttaaa acctcaatgt ctttaactaa accacacttc ttacaaaaaa
    58261 tagatttaat ttccaatcca tttatcgata acttagactt tttaatcttc atgtactgtg
    58321 tggcttctgt aaactcttta tttccgcatc ttttacattg cataaataat aactcctttt
    58381 atctataata caaatttatt gtaataatac ataacattaa ttttaattta aggtataatt
    58441 atatacaaat agtatcatta gggtgtgatt gagaattatg ggataataaa ataaagaagg
    58501 ctagagtgtt atgagtatgg acatgcaaga aaactacata gtagttatta tcagtaataa
    58561 ccgttagtat aacatttgaa gttgatatca aatttgaaat gattgattaa aaaaataaag
    58621 aatataaaag taattatggg gtcaattaga aattttgaaa gtgtcttgat taatatgtgt
    58681 agtttatttg tagaagttgg cattattttg ctgtttaaat acaataaaag gggataaata
    58741 catgacatta acagtggatt taaagaaaca gccatacgaa gtttttgatt taactgaaag
    58801 tgattgtttg catttaaaaa ataaaaattt tatttttggt aaaaatggta gaggaaaatc
    58861 cactcttact aaaatgatag aaaacgaata tagagaagga tataatattt t
//
LOCUS       DAHHRR010000003        50304 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.003, whole genome shotgun sequence.
ACCESSION   DAHHRR010000003 DAHHRR010000000
VERSION     DAHHRR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 50304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50304
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.003"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            313..687
                     /locus_tag="NBI42_000137"
     CDS             313..687
                     /locus_tag="NBI42_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCD1776603.1"
                     /translation="MKRILVVFLMLAIVLAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKNGKVIVIGIQLYKDREKMYYFPYEIKDGKAEINSEID
                     PIKYMKDHKADYEDENVEVEKK"
     gene            837..1235
                     /locus_tag="NBI42_000138"
     CDS             837..1235
                     /locus_tag="NBI42_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD1776604.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLFLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(1451..2275)
                     /locus_tag="NBI42_000139"
     CDS             complement(1451..2275)
                     /locus_tag="NBI42_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCD1776605.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(2514..3821)
                     /gene="brnQ"
                     /locus_tag="NBI42_000140"
     CDS             complement(2514..3821)
                     /gene="brnQ"
                     /locus_tag="NBI42_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD1776606.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRVFGSFGNIV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFVLIFSTIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKWFMMLPLADIDLAWLVPFMLFAIIGFIIDVVIRRPKQATT"
     gene            4092..4616
                     /locus_tag="NBI42_000141"
     CDS             4092..4616
                     /locus_tag="NBI42_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HCD1776607.1"
                     /translation="MNVRFPIGQLKVPETITSAHIEGWTNEINEYSSKLGSIVNHLDE
                     EELNRTYRQGAWTVRQLVHHIVDSQLNLYLRLKLALTDNEPYVSEFNQEQWALQSDYD
                     LPVQVSVDLLRNLNIRVVALIKEISTTQLSRAFELQNTGRVTVGETIAKLSWHERHHL
                     AHIQIALSQFNEEC"
     gene            5156..6046
                     /locus_tag="NBI42_000142"
     CDS             5156..6046
                     /locus_tag="NBI42_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HCD1776608.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKVNLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEIK"
     gene            6295..7344
                     /locus_tag="NBI42_000143"
     CDS             6295..7344
                     /locus_tag="NBI42_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCD1776609.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFIVPSLIDGHKATKDNEVLADETLKNKGFKIGDTLTLSQSDEKLHIVGFT
                     ESAKYNASPVIFTNDATIAKINPRLSGDKINAVVVRDTNWKDKKLNQKLEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTLILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAVVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            7357..8034
                     /locus_tag="NBI42_000144"
     CDS             7357..8034
                     /locus_tag="NBI42_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1776610.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            8241..9272
                     /locus_tag="NBI42_000145"
     CDS             8241..9272
                     /locus_tag="NBI42_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD1776611.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            9615..10733
                     /locus_tag="NBI42_000146"
     CDS             9615..10733
                     /locus_tag="NBI42_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD1776612.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKDLVAETSNPVTSTRSIGGVARNIAEN
                     IGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAVDWIITNKDETETYLNLKIETTEDLKVAAKRWNDLGVKNIIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            10708..11631
                     /locus_tag="NBI42_000147"
     CDS             10708..11631
                     /locus_tag="NBI42_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCD1776613.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATNKDVAKVSRRDLAEIVAMK
                     RIGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QALNLEGGIVVANPIPREYALSKAYIKAIINEAVIEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            11642..12862
                     /locus_tag="NBI42_000148"
     CDS             11642..12862
                     /locus_tag="NBI42_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD1776614.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKNIDFRKTLIMIFVQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     NDVEIDNLTKSTETKSVNEKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIIFGSVDLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLISNEFVAMLDF
                     KNVLGNVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(12967..14499)
                     /locus_tag="NBI42_000149"
     CDS             complement(12967..14499)
                     /locus_tag="NBI42_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCD1776615.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLLIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYAHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARLVIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(14539..15420)
                     /locus_tag="NBI42_000150"
     CDS             complement(14539..15420)
                     /locus_tag="NBI42_000150"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCD1776616.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            15579..16439
                     /locus_tag="NBI42_000151"
     CDS             15579..16439
                     /locus_tag="NBI42_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD1776617.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQIPTPDNKSELITDKV
                     YEIVTGYMKQYHLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIDGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(16716..17516)
                     /locus_tag="NBI42_000152"
     CDS             complement(16716..17516)
                     /locus_tag="NBI42_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1776618.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            17657..18325
                     /locus_tag="NBI42_000153"
     CDS             17657..18325
                     /locus_tag="NBI42_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCD1776619.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILVIKEAVDLPVIGIVKRDYEHSDVFITATSKEVDELIESRCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(18452..19765)
                     /locus_tag="NBI42_000154"
     CDS             complement(18452..19765)
                     /locus_tag="NBI42_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCD1776620.1"
                     /translation="MNQYHSNAQQPSAWSFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAIIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPITNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNVLTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQAVLAKASAISIVEMFLPSLLVTKAAISTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVPLSLLITIPVALLIFG"
     gene            20183..22258
                     /gene="lip2"
                     /locus_tag="NBI42_000155"
     CDS             20183..22258
                     /gene="lip2"
                     /locus_tag="NBI42_000155"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HCD1776621.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVTSHEAQASEKTPTT
                     NAAAQKETLNQSGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQST
                     QNSKTIRTQNDNQVKQDSERQGSKQSHQNNATNKTERQNDQVQNTHHAERNESQSTTS
                     QSNDVDKSQPSVPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDASTDKHPNQQDT
                     HQPAHQIIDAKQDDNVHQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQA
                     PKTRSTTNAAADAKKIRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNK
                     FKVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGK
                     TYKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHQAHGGEISPLFTG
                     GHNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGF
                     KQLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVS
                     SHTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVT
                     NNEPATRRGIWQVKPILQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATE
                     GKGTQLKAS"
     gene            complement(22500..23327)
                     /locus_tag="NBI42_000156"
     CDS             complement(22500..23327)
                     /locus_tag="NBI42_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1776622.1"
                     /translation="MTEIKDKVITKDAFELPYTIIKAKHQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSGINTEPFKMANSYYAKIAQSVNETVLAQLTS
                     PTPIIQDQIAQRFLIYGYARGTGEWINMINIADYTDSKYNISPNELKTLPPVFVAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMA"
     gene            complement(23385..24599)
                     /locus_tag="NBI42_000157"
     CDS             complement(23385..24599)
                     /locus_tag="NBI42_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCD1776623.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKADL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIDHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKQ
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGEHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPQDIDLEFSMEDLENLAIPRAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDTSSKDNFS"
     gene            24695..24787
                     /locus_tag="NBI42_000158"
     CDS             24695..24787
                     /locus_tag="NBI42_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776624.1"
                     /translation="MENIFKIELMNGICRSENMNMKKKNTSEKI"
     gene            24901..25902
                     /locus_tag="NBI42_000159"
     CDS             24901..25902
                     /locus_tag="NBI42_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCD1776625.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNLGTTMVKQALDGINPKYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKYFQASTIKMD
                     AKVMASVFVIVADNEAEVAALHHALDVWLLGKLQFAEFEHFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEQVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            25936..26268
                     /locus_tag="NBI42_000160"
     CDS             25936..26268
                     /locus_tag="NBI42_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCD1776626.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            26269..27069
                     /locus_tag="NBI42_000161"
     CDS             26269..27069
                     /locus_tag="NBI42_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCD1776627.1"
                     /translation="METLKSNKARLEYLINDMRRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LPAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQQSLNHIAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFKRDAE"
     gene            27059..28003
                     /locus_tag="NBI42_000162"
     CDS             27059..28003
                     /locus_tag="NBI42_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HCD1776628.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDAAEYDMTHVFHIQGEYILRQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLQRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPKSIQDR
                     TIHLTDDISTLITTALRNDSTTKNNNIGETEDVLNRTD"
     gene            27981..29003
                     /locus_tag="NBI42_000163"
     CDS             27981..29003
                     /locus_tag="NBI42_000163"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCD1776629.1"
                     /translation="MYLIEPIRNGEYIADGAIALAMQVYVNQNIFLGEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDEHHIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVIQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDETYRDITIEAFKNLMVTQILGIN
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEQVLQGTKMTREDLLHQLKQLDIVYYFGNVTVESL
                     VEMILS"
     gene            complement(29147..30493)
                     /locus_tag="NBI42_000164"
     CDS             complement(29147..30493)
                     /locus_tag="NBI42_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCD1776630.1"
                     /translation="MQAILNFTVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAVAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATAILLKGRQKPQQ"
     gene            complement(30508..30792)
                     /locus_tag="NBI42_000165"
     CDS             complement(30508..30792)
                     /locus_tag="NBI42_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1776631.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLAEKLQQLNMI"
     gene            complement(30794..31237)
                     /locus_tag="NBI42_000166"
     CDS             complement(30794..31237)
                     /locus_tag="NBI42_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1776632.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDSKTVQQLLIATNAQDIKNILKEHD"
     gene            complement(31242..33197)
                     /locus_tag="NBI42_000167"
     CDS             complement(31242..33197)
                     /locus_tag="NBI42_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD1776633.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTANTTILNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNRENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATYKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAITVAVQPLINDGIVNSNYTAAILKQLEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            33409..33828
                     /gene="mepR"
                     /locus_tag="NBI42_000168"
     CDS             33409..33828
                     /gene="mepR"
                     /locus_tag="NBI42_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HCD1776634.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRKKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            33935..35290
                     /gene="mepA"
                     /locus_tag="NBI42_000169"
     CDS             33935..35290
                     /gene="mepA"
                     /locus_tag="NBI42_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HCD1776635.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVALPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMISMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIVYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMSNKGRMKDVIKTVIMSIGVIFVVCMIAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMTSLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            35393..35833
                     /locus_tag="NBI42_000170"
     CDS             35393..35833
                     /locus_tag="NBI42_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HCD1776636.1"
                     /translation="MYKSKILLKYIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKAGYFVTCWTKDENNCNQPYSKEAFADYLMIIVIDEELSGYFLFPRELLI
                     EKGILTTFEHKGKMAFRVYPKWCNQLNKRAEQTQKWQCKYFFEY"
     gene            complement(35917..37275)
                     /gene="glpT"
                     /locus_tag="NBI42_000171"
     CDS             complement(35917..37275)
                     /gene="glpT"
                     /locus_tag="NBI42_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCD1776637.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLVLT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            37586..38512
                     /locus_tag="NBI42_000172"
     CDS             37586..38512
                     /locus_tag="NBI42_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1776638.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSEHLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNESFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            38526..39587
                     /locus_tag="NBI42_000173"
     CDS             38526..39587
                     /locus_tag="NBI42_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCD1776639.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPFKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLNLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNTLPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYHHAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSPDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNEIIPAIKKHLPK"
     gene            39601..40167
                     /locus_tag="NBI42_000174"
     CDS             39601..40167
                     /locus_tag="NBI42_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD1776640.1"
                     /translation="MNIVLLSGSTVGSKTRTAMDYLKNELEVINEGHQIELMDLRDLE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEERDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVLEEENNQYDF"
     gene            complement(40227..41222)
                     /locus_tag="NBI42_000175"
     CDS             complement(40227..41222)
                     /locus_tag="NBI42_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCD1776641.1"
                     /translation="MASLKNKHFMIGLSFTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSRLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            41527..42072
                     /locus_tag="NBI42_000176"
     CDS             41527..42072
                     /locus_tag="NBI42_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1776642.1"
                     /translation="MFGMKVNEQIILKILEAHDTEALFNLVNRSRDSLREWLPWVDAT
                     EQPSDTHAFIKRGLLQFADGNGFQCGIWYEGRLAGVVGLHEINHMHRKTSLGYYLDKQ
                     FEGKGVMTQAVETLIKYCFEEIDLNRIEISAAVNNRKSQAIPERLGFTKEGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
     gene            42340..43194
                     /locus_tag="NBI42_000177"
     CDS             42340..43194
                     /locus_tag="NBI42_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HCD1776643.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKDLYEDNKIDDMTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQTNFDKVNQLLDKYKDKNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            43191..44420
                     /gene="efeB"
                     /locus_tag="NBI42_000178"
     CDS             43191..44420
                     /gene="efeB"
                     /locus_tag="NBI42_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCD1776644.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSVWSFKS
                     MFNTPEDPEKDAYEFHGKVQPGITTPTQKTCHFVALDLKSKDRDAIKSMFKKWTVMAD
                     RMMDGDTVGKASKNPLMPPVDTGEAIGLGASKLTLTFGISKSMMKKIGLSGKIPDTFK
                     DIPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDVVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKSNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDERTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            44401..46113
                     /locus_tag="NBI42_000179"
     CDS             44401..46113
                     /locus_tag="NBI42_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HCD1776645.1"
                     /translation="MKHYLIKIVATIITLVMVFSFGLLKSQGVEQHSISDVYSVITDA
                     KSALSSNKISNDSKKDAVKKVVSSVGKLSLDDNSEGNAVKSDLRKLKDAKSNADQTDA
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQIDGKDRAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSSASESNQFHVSQLNDELDKAIKAIDSNQLSDADTALTHFIEIWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKSHVKDGLVDINNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVNDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESVEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQVIYIIIVAFLIF
                     KFKK"
     gene            complement(46205..46903)
                     /gene="tatC"
                     /locus_tag="NBI42_000180"
     CDS             complement(46205..46903)
                     /gene="tatC"
                     /locus_tag="NBI42_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HCD1776646.1"
                     /translation="MVHFSELRHRLVKILLSFIIAVIIVYVSSFWWMTSVISCITRAN
                     VTLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSLFS
                     VLLFIFGVAFAFYIGFPMIIQFALKLSTTLNISPVIGFKAYLVELIRWLFTFGILFQL
                     PILFIGLTKLGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     MFIVKFTCRVKPPH"
     gene            complement(46923..47150)
                     /locus_tag="NBI42_000181"
     CDS             complement(46923..47150)
                     /locus_tag="NBI42_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HCD1776647.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQRERD"
     gene            complement(47249..47638)
                     /locus_tag="NBI42_000182"
     CDS             complement(47249..47638)
                     /locus_tag="NBI42_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCD1776648.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQNGTA
                     TYVHQSEDDIVTSSVKSTIPVAQKSNKTMVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLKEQAIISELIPQ"
     gene            47913..48116
                     /locus_tag="NBI42_000183"
     CDS             47913..48116
                     /locus_tag="NBI42_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1776649.1"
                     /translation="MRNRLKELRARDGLNQTQLAKLAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETIFIIEEDEA"
     gene            48113..48826
                     /locus_tag="NBI42_000184"
     CDS             48113..48826
                     /locus_tag="NBI42_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HCD1776650.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGLGIENLTFATYNNVV
                     VISIVATIIIILVEGIILMNQRRALKYKRLVDEEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAAANAMLFFLIPFFASAIFNTQFTLFNRRFDDRMPKIADK
                     NYTEKRLEILDEGERHIELVALFKTYAINLSLLILAIIFIGSYSIATGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            48851..49693
                     /locus_tag="NBI42_000185"
     CDS             48851..49693
                     /locus_tag="NBI42_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1776651.1"
                     /translation="MTTLLNVDRVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSIAFFGEEHAADDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QNIYTTWNSQKFFDLIKYFELPQQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMEILEDFVAQGGGILISSHISEDIEHLADKLVFMKDGKIILTEQKDIL
                     LAQYGIVTTEDKDVEIPNHLMIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            49693..>50304
                     /locus_tag="NBI42_000186"
     CDS             49693..>50304
                     /locus_tag="NBI42_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCD1776652.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIVYAVIGIVITSVSYILSIKIF"
BASE COUNT        17491 a         7612 c         9562 g        15639 t
ORIGIN      
        1 aatatttggt tatgaaataa atgtgtggga ggcaattttt ggagatacat agcaatgtaa
       61 aggtatcaaa tacttattgc ataatttatt atgatgacat aattttcagt agataattgt
      121 aaattaatac tgaaatagat gtttatatta agaagacttt aatatcataa ttaatcaaaa
      181 tcttgtgtca aacatttcaa cgaattttaa gtttataaca attggttaaa cattgttcaa
      241 acatcacaat gataaagcat attattagta ttgtagtgtg tggaaaatga cagccatcta
      301 aggagaaaaa tgatgaaaag aatattggta gtatttttaa tgttagcaat tgtattagca
      361 ggttgttcta ataaaggtga aaagtatcaa aaagatattg ataaagtgta caaagaacag
      421 aatcaaatga ataaaattgc ctcgaaagta caaaacacta ttaaaacaga cattaaacaa
      481 gaagacagta atacacatgt ctataaaaat ggtaaagtca ttgttattgg tattcaattg
      541 tataaagatc gtgaaaaaat gtattatttc ccatatgaaa taaaagatgg taaggcagag
      601 attaatagtg aaatagaccc aattaagtat atgaaagacc ataaagcaga ttatgaagat
      661 gaaaatgtag aagtggaaaa aaaataacgt atgctttctt taaaactttt aaccagtttc
      721 aaaaaataaa tgagctttag cgataataat ttttacaata aacacgatat aaatttaaat
      781 atttatatat aagaacacaa acgtaagttt gtaaataata ataaaggagt aagacaatgg
      841 aaaaatcgat caaaataatg acaataatag gaattgttgt tcagggttta gcaacggtat
      901 ttagtttact attgatggtt ttagcagcat caggtgtaat gactacagat gtgtcaacaa
      961 cagttaatgg tgaggttgac ccagttgatg cagaaacagc agcagcaatt ttcactgtat
     1021 tattcttatt cctatttata tttggaatca tttcaattat tttaggtgca atcggtatgt
     1081 ttaaagcatc taaaaacaaa aaaatgagtg gtatattgtt gattattgga gctgtaataa
     1141 gtggtaacat aattacattt gctttatggt taatcagtgg tattaaacta cttactaata
     1201 acaagcctaa agatgaaata agcgacttat cataaacatc gtatattgaa atttcaagat
     1261 ttcttaagta attaaataaa gtgctctctt agaagtagat tttcagtcaa taactgcttt
     1321 taagagagca ttttgtattt gataatgctc atagcatcgg acaaaaacca ttgacgaact
     1381 ttaaaaaaat acgttagaca atgcacttct aaatattaaa ggcatcgact aacgcattgt
     1441 tcaaatagag ttaattattt ttataaaatt gatgatgatc attcaagtaa gcatagaata
     1501 aacctataat gagtccgcct ccaatatagt taccgataaa agccgcagcg atattcgaaa
     1561 tagctggtat gaagtgcaat gtatctactt gataaattaa accacccata aataagcaac
     1621 tgttgtaaac gacgtgttca taacccataa aggcgaatat ggtaacaccg aacatcatga
     1681 caaacatttt tgcgagtaca tcgtcaattt gcatggcaat aactaatgaa atattgataa
     1741 agaaattggc gaatatcgct ttcattaata tacttacaaa accagtagac aacgttttat
     1801 gctcaataac tgctgataac tgatttaaca tatctggcgt cattacattt gaaaaacgca
     1861 tgaaactaaa taaaatagca gcacctaaaa tatttcctgc aaagcacaat aaaaatattt
     1921 tcaatactct agttggttta attactttat aatacaggcc tacagtaaag tacatgaagt
     1981 tactggttag tagttcggag tttgtaaata aaatgagtac taacgcaaag ctgaatgtaa
     2041 tcgcactggc catattcaca atgcctggcg gtaaatctgg ttcgtgtgtt gctttaactg
     2101 ataatacgaa gaccgtaata atcccgataa taaatcctgc catcatagcg cgtaatagat
     2161 aacgttttaa ataaacgctt tgtaatatat ctttcgttct tatcgtttcg actacgttat
     2221 ttacccagtc gtccccataa aatattttat cccatttaat atgtttctcc ttcacaatga
     2281 catcctcact tttcatttgt taatcagagt atactatgaa ttgccttatt tgtgaataat
     2341 ttcacaaaat taattttaaa aactaatatt tttgaataac agctaggatt ttttaacaaa
     2401 tcatttattt taaaataatt aatcgtcagt tagtaacaat gaataaatac agattttcat
     2461 acaaaaaaac ctagtaatga tgcgacgatc attactagga gattatgcgt ctattaagtt
     2521 gtcgcttgtt tcggacggcg tataactaca tcgatgatga aacctataat agcaaagagc
     2581 atgaatggta caagccaagc taaatcgata tctgctaaag gtaacatcat aaaccatttc
     2641 aaaataacac cgtgtaataa gttgaaactg tttagtattt gtaaaattga aataatcaat
     2701 gtaataacag ttgcgagtcg ataggcccaa ctgaatctga atgtgctaaa catgttagca
     2761 aatgatatca gtacaagtgc aatcgacacg ggatatatta atgtcaacaa tggtacagca
     2821 atttttaaaa tcatttctaa accaagtgtt gtaaataaga accctatggt agagaaaata
     2881 agtacaaata ttttataaga aaacttaggt acgtgtttct tagtaaatgt ggcgcaagca
     2941 ttgacgagtc ctatacatgt tgttaaacat gcaagtatca ctgtcatacc aaatactatg
     3001 ttgccgaatg aaccaaatac tcgtaatgag ttgtacgtca atatatctgt accatcttta
     3061 aagtttcctg gagccgttga tgccccaacg tatgcaagtg caaagtaaat cattccaagt
     3121 aatatggctg caataagacc tgaaaagcag acatatttta aaattttcat gcgatctgtg
     3181 agccctttaa acttatagcc attgacaatg actacggaaa aagctaacgc agcaacaaga
     3241 tccattgtaa aatagccttc caaacttcct gaaatgaaag gatgtgttat atatttatcc
     3301 ttaggtgcac ttagtgcaga ttcagggttg aaaatgacag caatacttaa tagagcgacc
     3361 attaatagta ataacggtgt taataattta cctaaattat cgacgatttt cgatggattt
     3421 aaactaatcc agtaaacgat agcaaaaaag attgctgcga atataattaa agtccattgg
     3481 ttatgcacag gtaaaatgtg tcttgtacca atttcgtacg cgacatttgc agcacgtgga
     3541 ataccgtaaa atgctccgat agacatgtaa atcacgacag caaaaataaa cccgaaccat
     3601 ggatgtatac gattgcctac actttcaaca ccttcatcat aaaatgcaac aacaataaca
     3661 gtaataaagg ggagtaatat gcctgtaaga gcaaagccta gcataccaat ccacatattt
     3721 tgacccgctg tatggccaag catgggcggg aatattaaat ttccggctcc aaaaaatagt
     3781 gaaaataaca tgaggcccga aataataact tgttttttca atgtaatttc cttcctaaaa
     3841 ctagatattc ataataattt aaaaaatctg aaaaataaaa acgttcttac tttatcttta
     3901 aatcacaata ctatgattct attattttat aaaaaagatt gcaataataa cgcattaaga
     3961 aaaagcataa taacagtgta aattatctgt ttttaattga attatgctgt gctaatccct
     4021 ctaaatatat gtttgcattc ttcagaaacg aaagtgtaaa atgaaaataa gacgtttaag
     4081 ggggaaagta tatgaatgta agatttccaa ttgggcaatt aaaggtacca gaaacaatta
     4141 catcagcgca tatagaagga tggacgaatg aaattaatga gtatagttca aagcttggta
     4201 gtattgtgaa tcatttggat gaagaggaac tgaatagaac ttatcgtcaa ggagcttgga
     4261 ctgttaggca attagtacat catattgttg attctcagct caatttatac ttacgattaa
     4321 agttagcttt aactgataat gaaccatatg tttcagaatt taatcaagaa caatgggcgt
     4381 tacaatcgga ttatgatttg cctgttcaag tgtcggttga tttacttcga aatttaaata
     4441 tacgagttgt tgctcttatc aaagaaatct caactacaca attgtcacgt gcatttgaat
     4501 tacaaaatac aggtcgtgtc actgtaggtg aaacaatagc taagttatcc tggcatgaaa
     4561 gacatcacct tgcacatatc caaattgctt tgagtcaatt taatgaagaa tgctaaacga
     4621 tggattatgc aaaataatga actaactggt atgtaaatat tattggtagt gtgagagaag
     4681 cgagagacag cacagataaa gaaccaataa tgatttatta tgtagtggtt cttatacatt
     4741 agttatagct aatgtgtact taaaaataga aaaacatgag taaatcacat gcataaggaa
     4801 tactaatttc taaagaaaaa gtatttcttt atccttttga aattctctat gtttgtggtc
     4861 ccgccaactt gcattgcctg tagaaatttg gcccaatttc tctatgtttg ggcccttgtc
     4921 aagaattgta aaagcttgtt acaagcgcat tttcattctg tcaacaactg ttaatatata
     4981 attgtatcct gtgacattga tacatgtccg agactctact cgattgcatg aaatgttcac
     5041 atatttgtta gcttttcaag aaataatatt aaatatcttt tcatagtata gcaatattaa
     5101 ataaatgcat ttataatttt aactgtattt taaatattaa tcatgaggtg gtaagatgaa
     5161 taaaatttca aagtatattg caatagcatc attatcggta gcggttacag tttcagcacc
     5221 acaaacgaca aattctacag cgtttgccaa aagttctgct gaagttcaac aaacgcaaca
     5281 agcttctata ccagcatcgc aaaaggtgaa tcttggtaat caaaatatta tggcagtggc
     5341 ttggtatcaa aattcagctg aagcaaaagc attatattta caaggttata acagtgcaaa
     5401 gactcagtta gataaagaga ttaaaaagaa taaaggtaaa cataagttag ctattgcttt
     5461 ggatttagat gaaacagttt tagataattc tccatatcaa ggctatgcat caatacataa
     5521 taaacctttc ccagaaggtt ggcatgaatg ggtacaagct gctaaagcta aacctgtcta
     5581 tggcgcaaaa gaattcttga aatatgctga caaaaaaggt gtcgatatct actatatttc
     5641 tgatagagat aaagaaaaag atttaaaggc aacacaaaag aacttaaaac aacaaggtat
     5701 ccctcaagct aagaagagtc atattttact aaaaggtaaa gatgataaga gtaaagaatc
     5761 acgcagacaa atggttcaaa aggatcataa acttgtcatg ctatttggag ataatttatt
     5821 agactttaca gatccaaaag aagctacagc tgaatctcgt gaagcattaa ttgaaaagca
     5881 taaagacgat ttcggtaaga aatatattat tttccctaac ccaatgtatg gtagttggga
     5941 agctacaatt tataacaata actataaagc aagtgacaaa gcaaaagata aattacgtaa
     6001 aaatgctatt aagcaattcg atcctaaaac aggcgaaatt aaataatata tgaattggac
     6061 gtctacatgt acttaaaggt atatgtaggc gtttttaaat tgatccttaa ttttaaatgt
     6121 accgtacaca aataatagac aattaattgc attttccata aatttgcttt taatataaaa
     6181 agttcgggta taatgaaaga taagtgtatg tataacatta cagagcgcaa cacgacatac
     6241 gtgcatctaa gaatataaac aatgtttaaa tcatagtagt agggagagaa atgcatgttt
     6301 ttagcttgga atgaaatacg gcgcaacaaa ttgaagtttg gactaattat tggtgtgtta
     6361 acgatgatta gttacttgct atttttatta tctggattgg cgaatggtct tatcaatatg
     6421 aataaagaag gcattgataa gtggcaagca gatgccattg ttctaaataa agatgccaat
     6481 caaactgtgc aacaatctgt ttttaacaag aaagatattg aaaataaata caagaagcaa
     6541 gctactttga agcaaacagg ggaaattgtg tctaatggtc atcaaaaaga caatgtctta
     6601 gtgttcggcg ttgaaaagtc gtcttttata gttccgagtc taatcgatgg gcataaagca
     6661 actaaagata atgaagtgtt agcagatgaa acacttaaaa ataaagggtt taagattggt
     6721 gacacattaa cactatctca atctgatgaa aaattgcata ttgtaggttt tacagaaagc
     6781 gcaaagtata atgcatcacc agtcattttt acgaatgacg ctactattgc taaaattaat
     6841 cctcgattaa gtggcgataa aatcaatgca gttgttgtgc gtgatacaaa ttggaaagac
     6901 aaaaaattaa atcaaaaact tgaagcagta agtattaatg actttattga aaatttacca
     6961 ggttataaac cacagaactt aacattaaac tttatgattt cgttcttatt tgttatttca
     7021 gcaacagtga ttggtatttt cctatatgtc atgacattac aaaagacgag tttatttggc
     7081 atattaaaag ctcaaggatt tacgaatggc tatttggcga atgtggtaat ttcgcagacg
     7141 ctcatattag cattatttgg tacggcattt ggcttactgt taacaggcgt tacaggtgca
     7201 tttttacctg atgcagtacc tgtcaaattt gatgtactaa cattactcgt atttgcagtt
     7261 gtgttaatga ttgtctccgt attaggcagt ttattctcca ttttaacaat tagaaaaata
     7321 gatccgttaa aggcgattgg gtaggaggtg tagcaaatgt tgaaatttga aaatgtaaca
     7381 aagtcattta aagatgggaa tcgtaacatt gaagcggtta aagatacgaa ttttgagata
     7441 aataaaggtg atattatagc attggttgga ccttctggct ctggtaaaag tacatttcta
     7501 actatggcag gtgctttaca aacgccgaca tctgggcaca ttttaatcaa taaccaagat
     7561 attacgacaa tgaagcaaaa agcattggca aaagttagaa tgtctgagat aggttttatt
     7621 ttacaagcta caaatcttgt gccattttta acggtaaagc aacaatttac attattgaaa
     7681 aagaaaaata agaatgttat gtctaatgaa gactatcagc aacttatgtc acaattaggg
     7741 ctaacatcat tgcttaataa gttaccttca gaaatttcag gtggtcagaa acaacgtgtg
     7801 gccatagcca aagcgttata tacgaatccg tcgattattt tagcggatga acctaccgcg
     7861 gcgttagata ctgaaaatgc gattgaggtc attaaaattc tacgtgatca agccaaacaa
     7921 agaaagaaag catgtattat tgttacacat gatgaacgac ttaaagcata ttgtgatcgt
     7981 tcatatcata tgaaagatgg cgtccttaat cttgaaaatg aaacagtaga atagttttat
     8041 taagccggta catcatgtgc cggtattttt atgtttatgt attatttgaa taaactttca
     8101 cattcaatta ataataatta ttatcgaaaa ttagaaatat tccgtgaaat ataatatttt
     8161 ttgtagtaaa atggcctcta agtattcaat atttgactat ggggattgaa tataaattat
     8221 cataatgggg gtcatgctta atggatttat taataggtac tttattttta tttttagtct
     8281 tagtcatttt tacattattt acatataaag cacctaatgg tatgcgtgcc atgggagcat
     8341 tagctaatgc agcaatcgca acatttttag tagaagcatt taataaatat gttggtggcg
     8401 aagtattcgg tattaaattt ttagaagagc taggagacgc tgcgggaggt ctaggtggtg
     8461 tcgctgccgc tggattaaca gcattagcta tcggtgtgtc accagtatat gcattagtta
     8521 tagcagccgc gtgcggtggt atggatttat taccaggttt ctttgcgggt tatatgattg
     8581 gatatgtgat gaaatataca gagaaatatg tgccggatgg tgtcgactta attggatcga
     8641 ttgtcatctt agcgccatta gctcgtctta ttgcagtatt attaacgcca gtagtgaata
     8701 gtacattgat tcgaattggt gatattatcc aaagtagtac gaatacgaat ccaattatca
     8761 tgggtatcat tttaggtggt attatcacgg ttgtcggcac agcgccattg agttcaatgg
     8821 cattgacagc tttgttaggt ttaacgggtg tacctatggc tattggtgcc atggcagcat
     8881 ttagttcggc atttatgaat gggacgctat tccatcgctt aaaattaggt gatcgtaagt
     8941 ctacgattgc agtaagtatt gaacctttat cacaagcaga tattgtatca gccaatccaa
     9001 ttccaatcta tattacaaat ttctttggtg gtgcgattgc tggtttaatt attgctatgt
     9061 caggtttaat taacgatgcg acaggtacag ctacaccgat tgcaggattt ttagttatgt
     9121 ttggatttaa tcatccgacg acaattgtga tttatggtgt agtaatggcg attgtaggtg
     9181 cgcttgcagg ttatcttggc tcaattgtat ttaaaaaata tccaattgtt actaagcaag
     9241 acatgattaa tcgaggtgca gtagacgcat agcatcatca tattgaatag taaaaacaaa
     9301 taaaacatag taacgtgatt cagtcgatgt aacagtcgat aatgagtcac gtttttttat
     9361 agaaaaatat aagacataaa aatgtcataa tttatagtca acaaatatca tactgtataa
     9421 acatttatca ttttctcaag tgccttttac gcgatggaat gaaattactt tttacgaaat
     9481 tatgcgtatt ttataaacaa atatcattga tataacgata aatgtaagcg tttacaacag
     9541 aaataactgc atgctacgat atttttgtaa attcactgat tcaattattt taagtcaata
     9601 tgaggaggga tgttatgagc gattctgaga aagaaatttt aaaaagaatt aaagataatc
     9661 cgtttatttc acaacgtgaa cttgctgagg caattggatt atctagaccc agcgtagcaa
     9721 acattatttc aggattaata caaaaggaat atgttatggg aaaggcatat gttttaaatg
     9781 aagattatcc tattgtttgt attggcgcag cgaatgtaga tcgtaagttt tatgtgcata
     9841 aagatttagt tgcagaaaca tcaaatcctg taacgtcaac acgctctatt ggtggcgtag
     9901 caagaaatat tgctgagaac ataggtaggc ttggcgaaac ggtcgctttt ttatctgcta
     9961 gtggacaaga tagtgaatgg gaaatgatta aacgattgtc cacaccattt atgaatttgg
    10021 atcatgttca acaatttgaa aatgcgagta caggttcata tacagcttta attagtaaag
    10081 aaggcgacat gacatatggc ttagcagata tggaagtgtt tgactacatt acgcctgaat
    10141 ttttaattaa gcgttcacac ttattgaaaa aggctaaatg catcattgtt gatttaaatt
    10201 taggcaaaga agcattgaat ttcttgtgtg cctataccac gaaacatcaa atcaaattag
    10261 ttattactac cgtatcttcc ccaaaaatga aaaacatgcc agattcattg catgctgttg
    10321 attggattat cacaaataaa gatgaaacag agacatactt aaatttgaaa attgaaacta
    10381 ctgaagattt aaaagtagct gctaaacgat ggaatgattt aggtgttaaa aatattattg
    10441 tgacaaatgg cgtgaaagaa ctcatttatc gaagtggtga ggaagaaatc atcaagtcag
    10501 ttatgccatc aaatagtgtg aaagatgtca caggtgcagg tgattcattc tgtgctgcag
    10561 tagtgtatag ctggttaaat gggatgtcta ctgaagatat attaattgct ggtatggtta
    10621 acgcaaagaa aacgatagaa acgaaatata cagttaggca aaacctagat caacagcaac
    10681 tttatcacga tatggaggat tataaaaatg gcaaatttac aaaagtatat tgagtattct
    10741 cgagaagttc agcaagcacg ggagaacaat caaccgattg tagcattaga atcaacaatt
    10801 atttcgcatg gtatgccgta cccgcaaaat gttgaaatgg caacaacagt agagcaaatt
    10861 atcagaaata atggtgccat tccagcaacc atagccatta tagatggcaa aattaaaatt
    10921 ggtttagaaa gtgaagattt agaaatactt gcaactaata aagacgttgc taaagtgtct
    10981 agaagggatt tagcagaaat tgttgcgatg aagcgtattg gtgctactac tgtagcgacg
    11041 acgatgatat gtgctgcaat ggctggtatt caattttttg ttactggagg tattgggggt
    11101 gtccataaag gtgcagaaca tacgatggac atttcagcag acttagaaga actgtctaaa
    11161 acaaatgtca ctgttatctg tgcaggtgcc aaatcaattt tagacttacc taagacaatg
    11221 gagtatttag aaacaaaagg cgttccagtt attggatatc aaacgaatga attgccagca
    11281 ttcttcactc gcgaaagcgg tgttaagtta acaagttcgg ttgaaactcc ggaacgcctt
    11341 gctgacattc atttgacgaa gcaagcatta aatcttgaag gtggcattgt cgtagctaat
    11401 ccaattccac gtgaatatgc cttatcaaaa gcatatatta aggcaatcat aaatgaagct
    11461 gttattgaag ccgaaaatca aggtattaaa ggtaaggacg ccacaccgtt cttgttaggg
    11521 aaaattgtag aaaaaacgaa tggtaaaagt ttagcagcaa atataaaact tgttgaaaac
    11581 aatgcggcgt tgggtgctaa aattgctgtc gctgttaata aattgttgta ggtgatgata
    11641 catgaatatt ttattcgcta tcacagggat agcatttgca ctatttgttg cgtttttatt
    11701 tagttttgat cgtaaaaaca tagacttcag aaaaacatta attatgattt tcgttcaagt
    11761 gttgatcgtg ttatttatga tgaacacaac gattggtttg acaattttaa ctgcactagg
    11821 ttcatttttt gaaggattaa taaatattag taaagcaggt ataaactttg tttttggaga
    11881 tatacaaaat aaaaatggct ttacgttctt tttaaatgta ttgctgccat tagtgttcat
    11941 ttctgtatta ataggcattt ttaattatat taaggtatta ccatttatta tcaaatatgt
    12001 aggtatcgct attaataaaa taactagaat ggggcgctta gaaagttact ttgctatttc
    12061 aacagcaatg tttggtcagc cagaagtata tttaacaata aaagatatta ttccaagatt
    12121 atctagagcg aaattatata caattgcgac gtctggtatg agtgctgtta gtatggcaat
    12181 gctaggttca tatatgcaaa tgattgaacc taaatttgta gttacagctg taatgttgaa
    12241 tatttttagt gcacttatca ttgctagtgt tatcaatcca tataaatctg atgataatga
    12301 tgtcgaaatt gataatttaa ccaaatcaac ggaaacgaaa tcagtgaatg aaaaaacagg
    12361 gaagcctaag aaggtcgcct ttttccaaat gataggtgac agtgcaatgg atggatttaa
    12421 aatagccgtt gtagtagcag tgatgttgtt agcgtttatt tcattaatgg aagcaatcaa
    12481 tatcattttt ggtagtgttg atttgaattt taaacagctt attggctatg tgtttgcacc
    12541 aatcgcattt ttaatgggga ttccatggag tgaagctgtt ccagctggct ctttaatggc
    12601 gactaaatta atttcaaatg agtttgtagc aatgcttgat tttaaaaatg tcctgggtaa
    12661 tgtatctgct cgaacacaag gtatcatttc agtttactta gtaagcttcg ctaattttgg
    12721 tacggttggt atcatcgtag gttcaattaa aggcattagt gataaacaag gagaaaaagt
    12781 tgcatccttt gccatgaggc tgttacttgg ttcaactcta gcttcaatca tttcaggatc
    12841 aatcattggc ctagtattgt aaattaatcg aagtatctaa attaaattca tggcaaatca
    12901 aaaccccatc accaaggtgg cgcaacagcg catcataact tagtgacggg gttttatcat
    12961 aacaatctac tttttcgtag ccgtttttga aatgtatgtt gatggtttat ctttttcaaa
    13021 aattgttaat cccgttatat cttttttatg ttttgaaggg acaatgaagc taagtatata
    13081 agcaaagaca aaagcaactg taaatgaaat ggtagataca tagaaaggtg agttaccttt
    13141 gccaacacca ttatagacat aagcaaagat gatacccaat attaatccac aaataacacc
    13201 gaatgtattc gtacgtttag tgaaaatacc aactgcaaat acaccagcca atggaacacc
    13261 gaataatcca gttacaaaca agaataaatc ccataagtca tttgaattag aagcaattaa
    13321 gtatagtgac attccaaaac cgaaaatacc tgcaatgatg atgaccaaac gtgcaaagtt
    13381 aacttcgtgt cgctcgctac cttttccgaa gaagcgttgc ttaatgtcga ttgaaataca
    13441 agcagatata gaatttaaac tagatgaaat ggtagactgt gcagcggcga aaatggctgc
    13501 aataagtaat cctgctacaa atggtggcat ctcagtcaaa atgaaatatg gcactacaga
    13561 tgatgtattg aagccttttg gcaaaacagc ttcatgtgca taaaatgaat acagcattgt
    13621 acccatacca taaaataagg gtgctgaaat taaagctagg ataccatttg tccataacga
    13681 tttatttgtt tcttttaaac tatcagaagc ttgataacgc tgcacgacgt cttgactcgc
    13741 tgtgtattga tacaagttgt tgaaaatatt tcctaggaaa ataattggaa tggcagctgc
    13801 tgcagtattt agtttccaat tgtctgcact aattaatttt ttgtgctcaa tcgcatctgc
    13861 aaagacagtg ccaaaaccgc ctttgatatt cataacacca agaatgatga taactaaagc
    13921 gccgcctaat aaaatgacgc cttgaatgaa atcactccaa accacacctt cgaaaccacc
    13981 taaaaatgta tataaaatac atagtaaacc aacgagtgat gcaacgatat aagggttcat
    14041 gtctgataca gatgtaatcg ctaatgttgg taagtagata acgattgcta cacgccctag
    14101 atgataaact acaaatagta aagagccaat gacacgaatg ctaggtccga aacgtgcttc
    14161 tagatattca tatgcagatg ttacctttaa ctttttaaag aatggaacat agaaataaat
    14221 gagtaatgga ataattgcta cgatagcgat gttacctgca atatatgacc aatctgttaa
    14281 aaatgctttc tctggtgtag acataaacgt aattgcactt aatgtagtgg cataaattga
    14341 aaagccaact acccaagatg gcaagcgacc acttgcggta aagaaactat tcgtactttg
    14401 actcgcgcgc ttagtaaaat aaacgccaat gagtaacata gctagtagat aaatgataac
    14461 ggcaacccag tttagtgtgc caaatccaac ttctttcatg ggcaacatcc cctttacaat
    14521 gtattgattc tttgatgtct ataaatcgta ttttgcaatg agttgatcta atgtttgtcg
    14581 atgtgcttcg ttaaaaggtt tgaaaggtct ttttggtaat cctgcatcaa tgccacgatg
    14641 acgtaatatt tctttcaatg ttggataaat ccccattgat aacactgttt cgataatgtc
    14701 gtttgaatca tgttgtagtt ggtaagcttc ttgaatttga ccttgtcttg ctaagtcgaa
    14761 gatttttcta gcgcggcgac cattaacgtt atatgtagaa ccaattgcac catctacgcc
    14821 agaaatcgta gcttgaacta acatttcatc aaagccagat aagattaatt tgtctgggaa
    14881 tgcttttcta atacgttcga gtaggaagaa gttcggcgct gtatatttaa caccaacaat
    14941 tttttcgtga ttaaaaagct cgctgaattg ttcaatagaa atattcacac ctgttaaatc
    15001 tggtattgca taaataatca tattgttctg tgttgcttcg ataatatcga aatagtaatc
    15061 tctaatttct tcaaaagtaa atggatagta gaatggtgtt acggcagaaa gtgcatcata
    15121 accgagttct gtggcatatt ttccaagttc aatggcttca tttaaatcta acgaacctac
    15181 ttgagcaatc aatttcacct tatccccaac tgcttctttg gcaaccttga aaacttgctt
    15241 cttctgctct gtatttaata aaaagttttc gcctgagcta ccatttacat aaagaccgtc
    15301 taattcttca gtttcaatgg cattttgagc aatttgtttg agtccttgtt catttacttg
    15361 accattttca tcaaaaggaa cgagtaacgc tgcatataaa ccttttaaat ctttgttcat
    15421 tatgaagtcc ctccaaaaaa tcatttgata atatagttta cagctataat tgtaaacgct
    15481 atcataaaat gtaacaatat ctttttgaaa attgtagtca tatttatgta taattaatga
    15541 aaatattttt caaaatcaat agaaatggag tgagtaaggt gtattacatc gcaatcgata
    15601 ttggaggcac tcaaattaaa tcggcggtta ttgataagca attaaatatg tttgactatc
    15661 aacaaatacc aacgccggac aacaaaagtg agcttattac tgacaaagta tatgagattg
    15721 taacaggata tatgaagcaa tatcatttga tccaacctgt cataggtatt tcatcagcag
    15781 gcgttgttga tgaacaaaaa ggcgaaattg tatacgcagg gccaaccatt ccgaattata
    15841 aaggtactaa ttttaagcga ttattaaaat ccctgtctcc ttatgtcaaa gtaaaaaatg
    15901 atgtaaacgc tgcattacta ggcgaattga aattacatca atatcaagca gaacggatct
    15961 tttgtatgac gcttggtaca ggcattgggg gtgcgtacaa aaataatcaa ggtcatattg
    16021 ataatggtga acttcataag gcaaatgaag ttgggtattt attgtatcgt ccaactgaaa
    16081 atacaacgtt tgagcaacgt gctgcaacga gtgcattgaa aaaacgcatg attgacggag
    16141 gatttacgag aagcacacat gtgccagtat tgtttgaagc agctgaagaa ggtgatgata
    16201 ttgcaaaaca aatattgaat gagtgggcag aagatgtagc agaagggatt gcccaaatac
    16261 aagtcatgta tgatccaggg cttatattaa ttggtggcgg tatatctgaa caaggagaca
    16321 atctcattaa atatatcgag ccgaaagttg cacactattt accaaaagac tatgtttatg
    16381 caccgataca aacgactaag agtaaaaatg atgcagcatt atatggctgt ttgcaatgat
    16441 ggttgaaaga aggagtcatt ctaaaataga atttgaaacc gataagagag atgagagctg
    16501 ttgttagttc cacacatcac accctatcta ggaccaatct aaactatatc aaccaacagt
    16561 gtgacacggg catattaaat tgaagaggct tgaaagtata gttctcaaaa atgtaaaaaa
    16621 atatttggta ttgaaattaa aaaagcacct agcaactcgt tgggacaatc acgatgattg
    16681 tctacagttg caggtggatt tgaatatact actagttatt tgttgtctag gataatagat
    16741 ttagtatgtt gataagtttg actcagattc gtattttcta ataaatgata actcacgata
    16801 tcgattaaaa agagtgtcgc aatttgtgtg ttgataaatt gatggtcggt attacgcgat
    16861 tgatccgttg ttaaaagtac taaatctgca caatctgtaa gtttactacc ttcaaaattt
    16921 gtgatggcaa cgacatatgc accatgagat ttggcgactt ccgctgcaga aattaattcc
    16981 gaagtattac cactatttga catagcaata aacatatccg aatgagatag tagggatgcc
    17041 gatattttca ttaaatgtga atcggtagta acattacctt ttagccccat acgaatcata
    17101 cgataataaa attcagtcgc tgataaacca gagctaccta gtccagcaaa gagtatatgt
    17161 cgacttgatt gaagtttgtc gataaaggtt tggataatgt cgttatcaat aaattcacca
    17221 gtttgttgaa tgatttgttg atgatattta tgaattcttt gaatgattgg gctattttca
    17281 ataactgtct ctgtcatttc ttgttgaata ttaaatttta aatcttggaa attctcataa
    17341 tccagcttat gactaaagcg tgtcatcgtt gctggtgatg taccaatcgc atgggctaag
    17401 gagttaatcg ttgaaaaggc atcgctataa ccattttgtc ttatataatt gacgatgcgt
    17461 ttatcagttt ttgtaaataa atgttgataa cgttgaacac gattctcaaa tttcattgtg
    17521 tcaccccttc atcttaatga ttactattat atatgaaaaa tattttcaag atagtaaaaa
    17581 gcattgataa aaattatctt aatgatatat tgtaaatgac tttacgtgaa aaaaacaact
    17641 tatggagtga ggaataatgt taccacatgg attaatagta tcttgtcagg cactaccaga
    17701 tgaaccatta cattcatctt ttattatgtc gaaaatggca ttagctgcgt atgaaggtgg
    17761 tgctgttggt attcgcgcaa atactaagga agacatttta gtaattaaag aagcggtaga
    17821 tttaccagtt attggtattg tgaaacgtga ctatgaacac tcagatgttt tcattactgc
    17881 aacgtcaaaa gaagttgatg aactgataga aagccgatgt gaagtcattg cattggatgc
    17941 aacgttacag caacgtccga aagaaacgtt agacgaatta gtatcatata ttagaacaca
    18001 tgcacctaac gttgaaatca tggctgatat cgcgaccgtt gaagaagcta aaaatgcggc
    18061 acgacttggc tttgattata ttggcacgac gttacatggc tatactagtt atacgcaagg
    18121 acaattactt tatcaaaatg actttcaatt tctaaaagat gtactacaaa gtgttgatgc
    18181 aaaagtcatt gcggaaggta atgtcattac accggatatg tataaacgcg tgatggactt
    18241 aggcgttcat tgttcagtcg ttggtggtgc gataacacga ccaaaagaaa ttacgaaacg
    18301 ttttgttcaa attatggaag attaaatgat aacgataaaa aaacgagatg accatcatta
    18361 attaaaggta cctaattatc ttaggtggct gaatgaatgt aatgggttca tctcgttttg
    18421 tttgtttatg atagtgattt tattttcaac tttatccaaa aataagtaaa gcgacgggga
    18481 tagtgattaa tagcgacaat ggcacgcgta aaaaccaaat gatgatgagt ttccagacag
    18541 gtattttaat ttcagttgct agtatacatg gcactaatgc tgagaaaaag ataatggctg
    18601 atacgcttac tacaccgacg acaaatttag tactaattgc agctttagtt actaacaaag
    18661 atggtaaaaa catttctaca atagaaatcg ctgaagcttt tgcaagtaca gcctgatcag
    18721 caattgggaa aatataaata aatggataga atatatagcc aagccaatca atgaatggtg
    18781 tatagttcgc tacaatcagc cctaaaaaac caatcgataa tatagaaggc aaaataccaa
    18841 cagtcatttc taaaccgtct ttcaaattgt cccaaacgtt cttcacgagg gatggtgtta
    18901 atacattttg tttcatcgcc tctgcatatg cagttttcag tctgcttcct tcaatagcaa
    18961 cttcttgttc tccttcttgt ccgttataat attctgttga ttcattggtg attggcggta
    19021 gccatgcagt aattgcagtc acgacaaatg tgatgactaa agttatccaa aagtataaat
    19081 tccaatgcgg cattaatcct aaagttttag caacgataat cataaaagtt gctgaaactg
    19141 ttgaaaagcc agtcgcaata atcgtggctt ctcgtttgtt gtacatccct tgcttataga
    19201 cacgattagt aatcaataat cctaaggaat aactgccgac aaacgaagcc actgcatcga
    19261 cagcggattt acctggtgtt ttaaaaatag gtctcataat tggctccata taaacaccga
    19321 caaattctaa caagccatag cccactaata aagaaagcgc aattgcacct actggaatta
    19381 agatacttaa tggcatcatt aatttttcga acaaaaacgg accatagtta gctttaaata
    19441 gtattgatgg accgatttta aatacataca ttataccgat tattgcacct gcaactttaa
    19501 ataatgtaat gaccaagttt gtgattgaag tcataaaagt acgtctcact attggtaacg
    19561 ctgtaccaat taaaatcata atcagtgcaa catagggcat aagtggacct atgattgagc
    19621 gaatggctag atgaacatga tcgacgaaaa tagtgttgtt accattaatc gtaaaaggaa
    19681 taaagaaaca tagtatgccc actaaactat agacaaaaaa actccatgca cttggttgtt
    19741 gtgcattaga atgatattga ttcattaaag caaccccttt gtttaaatga atacacaaaa
    19801 ctgtatgatg caacttcccc ttaatgagat gaatcattat tttaatttag aaaaatctga
    19861 aaacttacta taattgtata gtttgaatta ttttcagacc aatacaaatt aactaattat
    19921 atatagattg aaactatatt acttaataaa atgtttatct taaatgttgt tgtgttgatt
    19981 caacgccgca actaaaagtg tttataaatt atttggaaat gcacatattt gtaaatgatt
    20041 agtatcgatt taatatcgta ttattaaatt tttattaatt ttgtagtctt aatcaaaaaa
    20101 taatatatgt catgttatat tgaaggtgca gttgtttttc attctcaaga gggggtcaaa
    20161 aaaatacttt tgaggtgatt atatgttaag aggacaagaa gaaagaaagt atagtattag
    20221 aaagtattca ataggcgtgg tgtcagtgtt agcggctaca atgtttgttg tgacatcaca
    20281 tgaagcacaa gcctcggaaa aaacaccaac gactaatgca gcggcacaaa aagaaacact
    20341 aaatcaatcg ggagaacaag ggaatgcgat aacgtcacat caaatgcagt caggaaagca
    20401 attagacgat atgcataaag agaatggtaa aagtggaaca gtgacagaag gtaaagatac
    20461 gcttcaatca tcgaagcatc aatcaacaca aaatagtaaa acaatcagaa cgcaaaatga
    20521 taatcaagta aagcaagatt ctgaacgaca aggttctaaa cagtcacacc aaaataatgc
    20581 gactaacaaa actgaacgtc aaaatgatca ggttcaaaat acccatcatg ctgaacgtaa
    20641 tgaatcacaa tcgacaacgt cacaatcgaa tgatgttgat aaatcacaac catccgttcc
    20701 tgcacaaaag gtaataccaa atcatgataa agcagcacca acatcaacaa cacccccatc
    20761 taatgataaa actgcaccta aatcaacaaa agcacaagat gcatccacgg ataaacatcc
    20821 aaatcaacaa gacacccatc aacccgcgca tcaaatcata gatgcaaagc aagatgataa
    20881 tgttcatcaa agtgaacaga aaccacaagt tggcgattta agtaaacata tcgatggtca
    20941 aaattcccca gagaaaccga cagataaaaa tactgataat aaacaactaa tcaaagatgc
    21001 gcttcaagcg cctaaaacac gttcgactac aaatgcagca gcagatgcta aaaagattcg
    21061 accacttaaa gcgaatcaag tacaaccact taacaaatat cctgttgttt ttgtacatgg
    21121 atttttagga ttagtaggcg ataatgcacc tgctttatat ccaaattatt ggggtggaaa
    21181 taaatttaaa gttatcgaag aattgagaaa gcaaggatat aatgtacatc aagcaagtgt
    21241 aagtgcattt ggtagcaact atgatcgtgc tgtagaactt tattattaca tcaaaggtgg
    21301 tcgcgtagat tatggcgcag cacatgcagc taaatacgga catgagcgct atggtaagac
    21361 atataaagga atcatgccta attgggaacc tggtaaaaag gtacatcttg tagggcatag
    21421 tatggggggt caaacaattc gtttaatgga agagttttta agaaatggta acaaagaaga
    21481 aattgcctat catcaagcgc atggtggaga aatatcacca ttattcactg gtggtcataa
    21541 caatatggtt gcatcaatca caacattagc aacaccacat aatggttcac aagcagctga
    21601 taagtttgga aatacagaag ctgttagaaa aatcatgttc gctttaaatc gatttatggg
    21661 taacaagtat tcgaatatcg atttaggatt aacgcaatgg gggtttaaac aattaccaaa
    21721 tgagagttac attgactata taaaacgcgt tagtaaaagc aaaatttgga catcagacga
    21781 taatgctgcc tatgatttaa cgttagatgg ctctgcaaaa ttgaacaaca tgacaagtat
    21841 gaatccaaac attacgtata cgacttatac aggcgtgtct tcacatactg gtccattagg
    21901 ttatgaaaat cctgatttag gtacattttt cttaatggat acaacgagta gaattattgg
    21961 tcatgatgca agagaagaat ggcgtaaaaa tgatggtgtc gtaccagtga tttcgtcatt
    22021 acatccgtcc aatcaaccat ttgttaatgt tacgaataat gaacctgcca cacgaagagg
    22081 tatctggcaa gttaaaccaa tcttacaagg gtgggatcat gtcgatttta tcggtgtgga
    22141 tttcctagat ttcaagcgta aaggtgcaga actcgccaac ttctatacag gtattataaa
    22201 tgacttgttg cgtgttgaag caactgaagg taaaggtaca caattgaaag caagttaaat
    22261 tcatcttcgg aatttaatat gctatgtaaa tcgtgctgtt atcatggtgc agcagacgta
    22321 agtagcatca cagtgttgaa ttttaaaata gtaaagtgaa ataaagcgcc tgtctcatta
    22381 gcgaaaacta aagggacagg cgtatctgtt tatgagctta ataaattgta tgaataatat
    22441 ggttgatcga ataactgttt atcatgatga taaattgagt ttttaaaaat aatgatatat
    22501 tacgccattg ttatagcatt taagaaatca acaactttac gataaatagt gattgcttcg
    22561 tcattaggtc tacgatcaaa atcatgctcg tttttattca cgcgttcaaa tgttgaatgt
    22621 ggaacatggt tcatgatatg ttcgctttcc tcaacgggaa catcataatc gccattacaa
    22681 tgcgcaacga acaccggggg aagtgtttta agttcatttg gtgagatgtt gtattttgaa
    22741 tcagtataat cagcaatgtt aatcatattg atccactcac ctgtaccacg tgcataaccg
    22801 tagattaaaa aacgttgggc gatttgatct tgaataatcg gtgttggtga agtaagttga
    22861 gcaagtactg tttcattcac actttgagct attttagcgt aatagctatt tgccatttta
    22921 aacggttcag tattgatgcc actataacca taaaaatcaa taacaccatc aatatctctg
    22981 tctcgtgcaa ttaatagact taaatatgca cctgatgatc tgccaaaggt aaaaataggg
    23041 caattagaat attgtgattg aatcgcatcg aatgatgcgt agacatcctc aataatacaa
    23101 tcgagactta cttctggtaa taaacgataa cttagttgaa ttaaatcgta atgttccgta
    23161 aggatatcga tatactgtgg cgataaatca ttggcttttc caaacattaa accgccacca
    23221 tgaatgtaga caatggcacc ttttgttggt tgatgcttcg ctttaataat tgtgtagggt
    23281 aattcaaatg catctttagt aattacttta tctttaattt cagtcacgac tatacagact
    23341 ccttattttt gaaattgatg tcattataac attgtcttaa atttctatga aaaattatct
    23401 tttgatgatg tgtcatgata attctgttct aattgacgta aagcgtcacg ggtatgcttc
    23461 tttagacctt ccccataatc catcatttta acaatatctt taaaagcggc acgcggaatg
    23521 gctaaatttt ctaaatcttc catagaaaat tcaaggtcaa tatcttgtgg tcgctgttca
    23581 gcaagtttat gcacaaagtc aggttctgtg acaaaaggcg aagacatgcc gaccatatct
    23641 gcatgttgta aagcatctaa agcagactct ggagaattaa tcccgccact tgcaattaaa
    23701 gggatgcgac cagctaaatg ttcatagaca atttggttaa ctggccgacc gaaatgttcg
    23761 cctggtgtac gcgacgtatt ttgataaata tgtcgacccc agctagcgat tgctaaatat
    23821 tggatgtttg aaacgtccat gacccaatcg attaattgat tgaactcgtc aatggtatat
    23881 cctaaatcac tacctctggt ttcttctggc gttgctcgaa atcctaaaat aaaattgtca
    23941 ggtgcttgtt tatcaatcac ttcttgtacc gcacgcataa cttctaaaca taatcttgca
    24001 cggtttttta atgagtcggc accgtaatgg tctgtacgtt tattcgaaaa agttgagaaa
    24061 aatgtttgaa tcagcaaacg ttgtgcaatc gaaatttcca caccatcaaa acctgcttta
    24121 atcgcgcgta atgtagcatc gcgatactgc tgaatgatgc tattgatttt ctcatgagac
    24181 atggcgataa catcgtgatc aatcggtgaa tgcaacgtca tagggcttgg tccatacacc
    24241 tttccaaaat ttaaaatggc ttgatttgaa aaacgaccag catgcgctag ctggataata
    24301 gcgaggctac catgttgttt catcgtagat gccatgttag ttaatccagg gatacaagca
    24361 tcatgatcaa tattaaagcc atattcaaac aattgaccat aaggttcaat gtaagcagcg
    24421 ccggtgactt gcattccagc tgaattagag cgacgtgcag cataagccaa gtctgctttt
    24481 gtaatatagc cttcttttgt tgatgtgttt acggtcattg gtgataatac aaagcgattc
    24541 gaaattttga tgccattagg taagtggatt gattgtaaaa gtggtttgta tcggtacata
    24601 ctatgattcc ttttctattc aatattgttt tcaaagtacc atggaaagaa tgaataatca
    24661 atgatgaaca gtcttgatag aatagaattg gtacatggaa aacattttta aaattgaact
    24721 aatgaatggc atttgtaggt ctgaaaatat gaatatgaaa aagaaaaata caagcgaaaa
    24781 gatataaaag ttaattgaaa aacgttatca tatacgtggg tatatgaaga gggaatggta
    24841 ttaagaacgc taaaatgtta tgtcggtttg acatgacagg ataagtttgg agatgacgga
    24901 ttggttaaat taagcgtatt agactatgcc ttaatagatg aaggtaagga tgcacaaaag
    24961 gcattgcagg attcagtgac acttgcaaaa ttagcagatc gacttggctt taagcgaatt
    25021 tggtttacgg aacatcataa tgtaccagcg tttgcgtgta gtagtccaga acttttgatg
    25081 atgcatacat tggcgcagac aaatcacata cgagttggct ctggtggtgt gatgttgccg
    25141 cactatcgcc cttataaaat tgctgagcat tttagaatga tggcagcgtt atatccaaat
    25201 cgtattgatt taggtattgg taataatcta ggtactacta tggtaaagca agctttagat
    25261 ggaataaatc ctaaatatga tagttacgat gaatcgattt cgttattacg tgattatctt
    25321 acaataaagg ataaaccaag tgcgcatacg ttaggtgtcc aaccacacat tgatcatttt
    25381 ccagaaatgt ggttattaag tagtagcgca acatctgcca aaatagctgc cgaactaggt
    25441 ataggacttt ctgttggaac atttttgcta ccagatataa atgcgataca tgcagcgaag
    25501 gataacattg atatttacaa aaaatatttc caagcatcaa cgattaaaat ggacgcaaag
    25561 gtgatggcat ctgtatttgt cattgtagct gataacgaag cggaagtagc agcattacac
    25621 catgccttag atgtttggtt attaggtaaa ttacaatttg cagaatttga acattttcca
    25681 tcggtagaca cagcgcaaaa gtataagctt aatgatcgag acaaagagat gattcaagca
    25741 catcaagcac gtatcattgc aggtacacaa gaacaggtta aagcacaatt agatgatttc
    25801 attgctacgt ttgaagttga tgaggtgtta gtagcaccac ttattccagg cattgaacag
    25861 cgttgtaaaa cattaaaatt actcgcggaa atctatttgt agcattttaa atagaagaga
    25921 aaggatggag ataagatgaa aaagttagcc aattatttat gggtagaaaa agtaggagat
    25981 ttgtatgtgt ttagtatgac acctgaattg caagatgata ttgggacagt aggttatgtt
    26041 gaattcgtga gtccagatga agttaaagtg gatgatgaaa ttgtgagtat cgaagcatcg
    26101 aaaacggtca ttgatgtgca aacgccattg tcaggaacga ttattgagcg aaatacaaaa
    26161 gcggaagaag aaccgacaat tttaaactct gaaaaaccag aagaaaattg gttgttcaaa
    26221 ttggatgatg tcgataaaga agcattcctg gcattaccgg aggcttaaat ggaaacgtta
    26281 aaatcaaata aagcgagact tgaatattta atcaatgata tgcgtcgaga gagaaatgac
    26341 aatgacgtat tggtaatgcc atcttcattt gaagatttgt gggaattata tcgaggttta
    26401 gcaaatgtca gaccggcatt acctgtaagt gatgaatatt tagctgtaca agatgctatg
    26461 ttaagtgatt tgaatcgtca acatgttacg gatttgaagg atttgaagcc aataaaaggt
    26521 gacaatatct ttgtttggca aggtgatatc acgacgttaa aaatcgatgc tattgttaat
    26581 gctgcaaata gtcgttttct aggatgtatg caagctaatc atgactgcat tgataatatt
    26641 attcatacaa aagcgggtgt tcaagttcga cttgattgtg cagaaatcat tcgacaacaa
    26701 gggcgcaatg aaggtgtagg taaagccaaa ataacacgtg gatataattt gccagcaaag
    26761 tatataattc atacggttgg tccgcaaata cgtcgattgc ctgtttcaaa gatgaatcag
    26821 gacttgttag ctaaatgcta tttgagttgt cttaaattgg ctgatcaaca aagtttaaat
    26881 catatagcat tttgctgtat atctacaggt gtatttgctt ttcctcaaga tgaagcagca
    26941 gaaattgctg ttcgaacagt agaaagctat ctcaaagaaa caaattcaac attgaaagtc
    27001 gtgttcaatg tatttacaga taaggattta caactgtata aggaggcatt taagcgtgat
    27061 gcagagtagt aaatggaatg caatgtctct tttaatggat gacaagacaa agcaggctga
    27121 agtattgcgt actgcgattg atgaagcaga tgcgatagtg attggaattg gtgcaggcat
    27181 gtctgcatct gacggattta catatgtagg agagcgtttt acggaaaatt tcccagattt
    27241 tattgaaaaa tatcgcttct ttgatatgtt gcaagcgagt ttacatcctt atggcagttg
    27301 gcaagagtat tgggcatttg agagtcgttt tattacatta aactatttag atcaacctgt
    27361 aggtcagtct tatctcgcat taaaatcctt agtggaaggt aaacagtacc atatcataac
    27421 tacgaatgca gacaatgctt tcgatgctgc tgaatacgat atgactcatg tatttcatat
    27481 acaaggggag tatatactgc gacagtgtag tcagcattgt catgctcaaa cgtatcgcaa
    27541 tgatgattta attcgtaaaa tggttgttgc gcaacaagat atgcttatac cttgggagat
    27601 gattccaaga tgtccaaaat gtgatgcccc aatggaagtg aataaacgta aagcggaagt
    27661 tggcatggtt gaagatgctg aatttcatgc gcaactacaa cgttataatg cttttctaga
    27721 gcaacatcaa gatgataaag tgttgtattt ggaaattgga attggttata ctacaccaca
    27781 atttgtgaag catccttttc agcgtatgac acgtaaaaat gaaaatgcac tttatatgac
    27841 aatgaataaa aaagcttatc gtattccaaa atcgattcaa gatcgtacaa ttcatttaac
    27901 cgacgatatt tcaacgttaa ttacaacagc actccggaac gacagcacaa cgaaaaataa
    27961 caacatagga gaaacagaag atgtacttaa tagaaccgat tagaaatggt gaatatattg
    28021 ctgatggtgc gattgcactc gccatgcaag tgtatgttaa tcagaatatc tttttaggtg
    28081 aagatatttt attcccttat tattgtgatc caaaagtgga aattggtcgt tttcaaaata
    28141 ctgctataga agtgaatcaa gattatatag atgaacacca tattcaagta gttcgtagag
    28201 atactggtgg tggtgctgta tatgttgata aaggtgcggt caatatgtgt tgtattttag
    28261 aacaagacac ttcaatttat ggtgattttc aacgatttta tcaaccagct ataaaggcat
    28321 tgcatacatt aggtgcaaca gatgtgatac aaagcggtag aaatgattta acattgaatg
    28381 gtaaaaaagt gtcaggcgcc gcaatgacat taatgaacaa tcgtatttat ggcggttatt
    28441 cgctattact tgatgttaat tatgaagcaa tggataaagt gttaaagcct aatcgcaaaa
    28501 agattgcatc gaaagggatt aaatctgtgc gtgcacgtgt tggtcatctt agagaagcac
    28561 tggatgaaac ctatcgtgat ataacaattg aagcattcaa aaatttaatg gtgacacaga
    28621 ttttgggaat caatgacatt aaagaggcaa aacgatatga attaacagat gcagattggg
    28681 aagcgattga tgaattagct gataaaaagt ataaaaattg ggattggaat tatggcaagt
    28741 cacctaaata tgaatacaat cgaagtgaaa gattatcttc aggtacggta gacataacaa
    28801 tttctgttga acaaaatcgt atcgcagatt gtcgtattta tggggatttc tttggacaag
    28861 gtgatattaa agatgtggaa caagtgttac aaggtacaaa aatgacaaga gaagatttac
    28921 tgcatcagtt aaagcaatta gacatcgttt attattttgg taatgttacg gttgaatcat
    28981 tggttgagat gattttaagt taatattgtt taggcgttaa tgtcgcaccc tcgttaaaca
    29041 ttatttaata taatacaaaa acaccgtcca taagtaagtg gctttcctct tgataaaagg
    29101 ggcattcact caatatgtga cggtgtttta tatttttacg ttaacactat tgctgtggct
    29161 tttgacgacc ttttaataaa attgctgtcg cacctacgat aataataaat agaatcgcac
    29221 caaataatcc catatatttt actgcgttac cgaacacgat accgacagct aaaaagtctg
    29281 tatctgagaa tgttgttgca gcaccaccta attcgcctaa aaatggcaag aataataatg
    29341 gtaaaaacgt gattaggata ccatttagag cggcgccagc aacagcacct ttaataccgc
    29401 ctcttgcatt accgaataca gcagccgttg cacctaagaa gaagtgtgca actacgccag
    29461 gtaaaatgac gacgccacca aataagaata agataaacat accgatgaca cctgtaataa
    29521 agctgacaaa gaatccaatt agtactgcat tttgtgcata agggaacaca atagggcagt
    29581 ctagtgcagg tttagaattt ggtacaagct tttcagaaat ccctttaaat gctgggacga
    29641 tttcagctaa gattaagcga acgcctgtta aaataataaa tacaccagca gcaaatgtca
    29701 cgccttgaat taatgaaaag acaataaagt tttgaccatc actaatagat tcgtgtacat
    29761 aactaacgcc cgcaaataag catgcgatga agtaaagtaa tgccatcgta atcgagatac
    29821 taattgtact ttctcgtaag aaacttaagc cttttggaaa tttaatctct tccgttgatt
    29881 tagacttacc tttgaataat tgacctacag cacctgcggc aaagtaactg attgagccaa
    29941 aatgacctaa cgctacttgg tcattccctg tgatttttcg catcgtaggt tggagtaatg
    30001 caggtaagac tgccatgatt aatcctaata cgagtgcgcc gataacaatc gttagccaac
    30061 ctttaatatg actgactgtt aaaatgattg ctaaaaacgc agccatgtaa aatgtatgat
    30121 gacctgttaa aaagatatat tttaaattag tgaagcgggc aattaaaata ttaacaatca
    30181 tgccacagac catgatgagt gcagctgttg ttccaaaatc ttttaaggct agtgagacga
    30241 tagcttcgtt gttaggtacg ataccttgta caccgaatgc gtgttggaat attttgccga
    30301 atggctcaag agatcgaacg acgacatctg cacctgcact taaaattaag aagcccaata
    30361 tcgttttaat ggttcctgaa gtgattgttg cggcaggttt tttctgaacg attaagccta
    30421 taaaggcaat cagtgcaaca agaatggctg gttgacttaa aatatcgact gtaaaattaa
    30481 ggattgcttg cataggtcgt acctccttta aatcatgtta agttgttgta atttttctgc
    30541 gagcttttgt tgtaattcag ctttgtctaa aatattatca agaactaaga catcccctag
    30601 acgttcggca ttttcagcta aatctctacc acaaataaac aagtcagcca tctctggact
    30661 tgctgtcata atgtcactat gttcaacttc gatatcagat ggtgcattaa gttgcctaag
    30721 tgcttcttgt gcgttcattt ctaccataaa actacttcct aaaccgtggc cacatactac
    30781 taaaattttc atattaatca tgctccttta aaatgttttt aatgtcttgt gcgtttgttg
    30841 caattaatag ttgctggact gttttgctat cgcccagtac ggttgctaaa ttttgtaata
    30901 cagataagtg tgaatgattg tcgatggcac tcaatacaaa aatgagtgat gcgtagtgat
    30961 cttcatcaca gaatgccaca tgttggttca actttaatag acttaaacca acttgatgta
    31021 cgtcattgtt cggtcttgca tgtgcaattg caatttcagg tgcgataacg atataaggtc
    31081 caagttcatt aacgctatca atcattgctt gaacatagcc ttgttcaata atttgttctt
    31141 gtagtaatgg ctgagaagct atagttatag cttcagtcca atcatttact tgttctttta
    31201 caatgatgcg tgttgttgac aaaatgtcta atgacacgtt attaagcctc ctttgtcata
    31261 gttaaagcaa tgtgttgttt aattttaaaa atattcccat ctaagaaatc ttgccgatat
    31321 aagtcgttgc ttaagcattc gctcaactgt cccaatgcct ttaaatgtgc attggggtgg
    31381 tccgttgcta atgtaattac aaggtgaacg ggatcgttag ctttactacc aaaaataatc
    31441 ccttcagtga aataagttag tgcgaaacct acaccattct gtacataatc agtaccagcg
    31501 tgaataagtg caatatgtgg actaatgacc atatatgacc cgaattgttc taattgtttt
    31561 aaaattgcag ctgtataatt tgaattgaca atgccatcat tgattaaagg ttgcacagcc
    31621 actgtaattg ccgattcaat tgataatggt tgtttattta taatgatgcg atgttcaggc
    31681 aataaatctg cgagtgactt gccatcagtt gccattttca tgactcgttg ttctcttgag
    31741 tcattgataa tttgattcaa tttttgacga gattgctgat tgataaatgg atcgacatga
    31801 ataactggta cagctgagat ttcacaaggt actgttgaaa tgacataatc aatgttatct
    31861 tgcaataatc gactttcttc caattgataa atggaatagg catcccaaat gtgaaactca
    31921 ggatacaggt gatttagttt tgattttaaa agttgcgatg tgcctatacc agaaccacat
    31981 agtaagacaa ccttaatcat tgattgttta tatgttgcaa cacgctctat acttgatgcg
    32041 aaatgaattg taatgtatgt taattcatct tcgttgaagc gaatagcagc atcttgttca
    32101 attgggctaa tatgcttgct aacggcttca atgatttgag gatagcgacg cataacttct
    32161 tgcctcaaag gattaggttg tagcatatcg tatttaatac gatgtatagc tggtttgata
    32221 tgtgtgatca gactggtatg caacttgttg tcttttgaca tatcaatgcc taattcttgg
    32281 ctaacacaag tgatcaattc atgtatattt tgcgataaat catggtattc aaaggtaatt
    32341 gaagatgctg tatgttcggt cattttagag cctagtaaat gtaacgtgat aaagataatt
    32401 tcagactctg gaaatgtgac attacaacta cgttctaagt tttctatcat ttttgaagca
    32461 atagcatact gattagtatg tcgccattta tcaatttcat tgataggtat atcgaacgaa
    32521 aaattttcat ttaaacgctg aatggcaatg agtatatgat agattaagcc atcgatagcc
    32581 gactgaacta aatgataatt ttctctattt aatgtcttaa taatggcacg gcgaaccaat
    32641 gcgattgatt ctgaattaaa gatatcagcc tctataaaag gtgcagcttg tttcatatat
    32701 tgatgtataa agtgtgcata cgctttacga taatgatctt cctcaccaat aatattgaat
    32761 cctttattgt ggacataatt taactttaaa tggtattgat ctagttgggc ttgaatcatt
    32821 ttaatatcat ctgcaattgt ccgacgcgaa acattaacat cttgtgcaag ttgctttgtt
    32881 gaaacaggat cagttgtttc gaataacttt aaagcgatat gtgtgagtcg ctcatctttt
    32941 gaaaaatgaa tttgattcgt tagattgtgc tctaattcat ttaatatcgt tgtgttagct
    33001 gttgttactt tgatgcctgc agctttatta cggctgactt ggtaatgata agtttcagca
    33061 tattgctcaa tatatgctat atcatattga atggtacgag gtgatacacc aagttgatta
    33121 gcaatggtat tgattggaat aaacgtttgc tcatgaatta aaagatacaa aatttcgatt
    33181 tgtctataac ttaacaacgt aatatcctcc tatttgtaat tgtaagcgat ttcttaaaag
    33241 cgtagatatg caatctcttt catattttaa tccgaaaaat tgcatatcaa aatgtttatg
    33301 gcgcaagatt ttataggaac ttttaaaata aattagatat tcatgttgac aatttaaaaa
    33361 tgtcgcagta tatttagtta gacatctaac gaaaaggtgg tgcaataaat ggaattcact
    33421 tattcgtatt tatttagaat gattagtcat gagatgaaac aaaaggctga tcaaaagtta
    33481 gagcaatttg atattacaaa tgagcaaggt catacgttag gttatcttta tgcacatcaa
    33541 caagatggac tgacacaaaa tgatattgct aaagcattac aacgaacagg tccaactgtc
    33601 agtaatttat taaggaacct tgaacgtaaa aagctgatct atcgctatgt cgatgcacaa
    33661 gatacgagaa aaaagaatat aggactgact acctctggga ttaaacttgt agaagcattc
    33721 acttcgatat ttgatgaaat ggagcaaaca ctcgtatcgc agttatctga agaagaaaat
    33781 gaacaaatga aagcaaactt aacgaaaatg ttatctagtt tacaataaat gttaagcgtg
    33841 actggtagaa atcagtcact ttatctttaa tgttatagtt agatatctaa ttgttagtaa
    33901 gctaattatt gggaaagaca aggagtattg aacaatgaaa gacgagcaat tatattattt
    33961 tgagaaatcg ccagtattta aagcgatgat gcatttctca ttgccaatga tgatagggac
    34021 tttattaagc gttatttatg gcatattaaa tatttacttt ataggatttt tagaagatag
    34081 ccacatgatt tctgctatct cactaacact gccagtattt gctatcttaa tggggttggg
    34141 taatttattt ggcgttggtg caggaactta tatttcacgt ttattaggtg cgaaagacta
    34201 tagtaagagt aaatttgtaa gtagtttctc tatttatggt ggtattgcac taggacttat
    34261 tgtaatttta gttgctttac cattcagtga tcaaatcgca gcaattttag gagcgagagg
    34321 tgaaacgtta gctttaacaa gtaattattt gaaagtaatg tttttaagtg caccttttgt
    34381 aattttgttc ttcatattag aacaatttgc acgtgcaatt ggggcaccaa tgatttctat
    34441 gattggtatg ttagctagtg taggcttaaa tattatttta gatccaattt taatttttgg
    34501 ttttgattta aacgttgttg gtgcagcttt gggtactgca atcagtaatg ttgctgctgc
    34561 tctgttcttt atcgtttatt ttatgaaaaa tagtgacgtt gtgtcagtta atattaaact
    34621 tgcgaaacct aataaagaaa tgctttctga aatctttaaa ataggtattc ctgcattttt
    34681 aatgagtatc ttaatgggat tcacaggatt agttttaaat ttatttttag cacattatgg
    34741 aaacttcgcg attgcaagtt atggtatctc atttagactt gtgcaatttc cagaacttat
    34801 tatcatggga ttatgtgaag gtgttgtacc attgattgca tataacttta tgtcaaataa
    34861 aggtcgtatg aaagacgtta tcaaaacagt tatcatgtct atcggcgtta tctttgttgt
    34921 atgtatgatt gctgtattta caattggaca tcatatggtc ggcctattta ctactgatca
    34981 agccattgtt gagatggcga catttatttt gaaagtaaca atgacatcat tgttattaaa
    35041 tggtataggt ttcttgttta ctggtatgct tcaagcaact ggtcaaggtc gcggtgctac
    35101 aattatggcc attttacaag gtgcgattat cattcctgta ttatttatta tgaatgcttt
    35161 gttcggcctc acaggtgtca tatggtcatt attaattgct gagtcacttt gtgctttagc
    35221 agcaatgtta atcgtctatt tattacgtga tcgtttgaca gttgatacat ctgaattaat
    35281 agaaggttaa atatttcgta cacttctagc tgagtatatt tcggtcgaaa gtgtattttt
    35341 cgaaaaaata aatatatgat acgattatga aaaaataaag tgagattagc atatgtataa
    35401 atctaaaata ctgttgaaat atatttttag tgaagaatca gaagttaaag atttaactga
    35461 agaaaaatat aatcaagatt acgaagcatt aacatttagc tttaaagagg aaacatatca
    35521 aagtaggtta gctaagaaaa caccgactaa agcagggtat tttgttacat gttggacaaa
    35581 agacgagaac aactgtaatc aaccatactc aaaagaggca tttgcagatt acttaatgat
    35641 tatagttatt gatgaagaat taagcggtta ttttttattt cctagggaat tgttgataga
    35701 aaaaggtatc ttaactacat ttgaacataa aggtaagatg gcttttagag tttatcctaa
    35761 gtggtgtaat caattgaata aaagagcaga gcaaacacaa aagtggcaat gtaaatattt
    35821 ttttgaatac taattaaaaa gtcatatggt tgaacttata agagatgaat atatcatctt
    35881 tcgataagat aaccatatga ctttattttt tatcatttaa tgatgaacgg tttcttgtcc
    35941 tactttattc caagtgagga taaagctcaa cattgcaaac acactgattg ctgttaataa
    36001 aataaaaccg acatcccatc cgaatttatc aactacagca cctaagacga tgttggccat
    36061 tacagcacca aacagataac caaataatcc tgttaatcca gctgctgtgc cagctgcttt
    36121 tttaggtaca taatctaatg cttgtaaacc aattaacata actggtccat atattaagaa
    36181 accaatggca attaatgaga cattgtctaa ccaagcattg cctggaggat ttaaccaata
    36241 aattaataca aatactgtga cacctaacat aaagaagaaa cctgcaggtc cacgacgacc
    36301 tttgaataat ttatcagaaa tgtaaccaca taataatgta ccaggaattc cagcccattc
    36361 gtataagaag tatgcccaac ctgatgcttt taagtcgaaa tgtttttctt cacttaagta
    36421 gactggcgcc caatcaagta caccataacg cacgaaataa acaaatatat ttgcaaaggc
    36481 aattgcccat acccatttat tgttcagtac atatttaaat aaaatttctt ttgtagttaa
    36541 ttctgtttct aatgttttct tatcgcttgt agcaaagtca tttttataaa tttcgattgg
    36601 aggtaaacct tgagattgag gtgtgtctct aatcaatacg tatgaaattg cggcaataat
    36661 aagtgctaag agtgcaggat aaatgaatac accttcgaaa ccttttaaat aaccaaagtt
    36721 gataaatgct gttgttgtaa taccccaagc agcaataggt gccataatac ctccaccaac
    36781 attatgcgca acgttccaaa gggcagtctt acttccgcgt tcacttacac taaaccagtg
    36841 aacgagaaca cggcctgaag gtggccagcc cataccttga aaccatccat ttaagaataa
    36901 taggacaaac ataataccga tacctgatgt aaagaacggt acaaatccca ttaacaaatt
    36961 gacgatagca gtgagtacta atccaagaac taagaatatc cgagcattgc tccgatcact
    37021 tacagtaccc ataaagaact tactaaatcc atatgcgatg gaaacagcag aaagcgcaaa
    37081 acctagttcc gcttttgtaa aaccttgctc ttgcaatgcc ggcatcgcta atgaaaagtt
    37141 tttacgtaat aaatagtacc cagcgtaacc gatgaaaata ccaagaaata cttggagacg
    37201 taatcgttta taggtatcat ctatctgatt ttctggcaaa ggcttaatat gctttgcagg
    37261 tttaagaaaa ttcataaaat cctccttaat atgtatttat atgcattttg tgcgataaac
    37321 ttatattaga catgtatgaa agcgttgtca atatactttt gtgaaatctg cagatttagt
    37381 ttttgaagta ttttataaac gtttagaaaa aataaattgc cacacataaa tttataatta
    37441 tgcaattatt atgtcgttat acatactagc tgtgtatcta cgaatgagtg caaacatatt
    37501 tttatttgca gaaaaggggt aaatggcata taactatctt ttttatgtta gttggtataa
    37561 aatttatact aacgggaggg atagtatgaa tatagtaggg catcatcaca tatccatgta
    37621 tacaaaagat gcaaaacgta ataaggattt ttacacaaat gtccttggat tacgattggt
    37681 tgaaaagtcg gttaatcaag acaatccttc aatgtatcat ttgttttatg gggacgaagt
    37741 aggtacagct ggaacaattt taagcttttt tgaaattccc aatgcgggtc ataagcagcc
    37801 aggtactgaa acgatttatc gattttcatt attagtacca aatcaagcgg cacttcatta
    37861 ttttgaaaaa cgtcttgaga ataatggtat taagtctgaa catttgtact atcttggaca
    37921 agaaggtgtt gtctttaaag atgaagacga cttagaaatc atactgcttg ttaatgatag
    37981 ttttgaagta ccacatcaat ggcaacataa cgcttatagt gaaatacctc aagcatatca
    38041 aattttagga atagggccag tcgaattaag agttagaaat gcagcgcgta cggtagaatt
    38101 tttggaaaat gtcttaggtt atcgcaaaag agataatgaa tcattcgatg tgctgacatt
    38161 agcaccacaa ggtttatatt cagattttgt agttattgag caacagggac aacgtgaaag
    38221 acctggacga ggttatatcc atcatattgc agttaataca ccacaaatga gtgacttaga
    38281 tgcaatttac aagaaattac aacaacaacc acaaagtaat tcaggtataa ttgatcgcta
    38341 tttctttaaa tcattatact atcgtcataa ttcaattatg tatgaatttg cgactgaagc
    38401 gcctggattt actattgata cacctgttga acaattagga agtcaattga acttgcctga
    38461 ctttttagaa gcagaacgtg aacaaattga aagtaagtta cacgaaatat aaaggagaat
    38521 gtttaatggc aaaattagaa atgaataaaa atacgcctct tgagtttggt ttgtattcct
    38581 taggtgatca tttattgaat ccatttaaag gtgaaaaagt tagttatgag caacgtatta
    38641 atgaaattat tgaagcaagt aaattagcag atgaagcagg tattgatgtt tttgcagttg
    38701 gtgaaagtca tcaggagcat tttacaacac aggcacatac ggttgtgtta ggtgcaattg
    38761 cccaagcgac aaagcatata aaagtttcaa gttcttcaac gattattagt gcaacagatc
    38821 ctgtaagagt atttgaagac ttcgcgacat tagatttgat ttctcatggt agagccgaaa
    38881 ttgtggctgg tagagcatca agaacaggta tttttgattt gtttggctat gatttaaaag
    38941 attatgatga attatttgaa gaaaagttaa atctactttt agagttaaat aaaacagagc
    39001 gcattacttg gtctggaaaa tatcgaccag aacttagaaa tatgaaaata ttcccaagac
    39061 caatcgataa tacattgcca atatggcgtg ctgttggtgg tccacctgca agtgctatca
    39121 aagcgggaaa acaaggtgtg ccaatgatga ttacaaccct tggtggccca gcaatgaact
    39181 ttaaaggttc tatagatgct tatcatcatg cggcaactga ggcaggtttc gatgcttcgc
    39241 ctaagtcttt accagtaagt acagcgagtc tgttttatac agctgaaaca actcaggatg
    39301 ctatgagaga attttatcca catttgaata cagggatgtc atttattcgt ggcgttggtt
    39361 atccgaaaca gcaatttgct aattcaccag attatagaga agcgctaatg gttggaagcc
    39421 cgcaacaaat tattgaaaag atattgtatc aacacgagtt gtatggtcat caacgcttta
    39481 tggctcagtt agattttggt ggtgtaccat ttgaaaatgt tatgaagaat attgagttaa
    39541 ttggtaacga aatcatacca gctattaaaa agcatttacc aaaataggag gatagtcatg
    39601 atgaatattg tattattgtc aggctcaaca gtcggttcta aaacgagaac tgctatggat
    39661 tatttaaaaa atgaattaga agtcatcaat gagggacatc aaatagaatt gatggattta
    39721 cgagatctcg aattagaatt tagcgttgga aagaattatc tagatactac aggagatgta
    39781 tataaattaa cgacgtcgtt aatgcaggct gatgtgattt ttattggttt tccaattttt
    39841 caagcttcca tccctggtgc tttgaaaaat gtatttgatt tacttccggt caatgcgttt
    39901 cgcgataagg taataggact tgtagcgaca gcaggttcta gtaaacatta tttaattcct
    39961 gaaatgcatt taaaaccaat attgagttac atgaaagcac atacgatgca aacgtatgta
    40021 ttcattgaag agagggactt ttcaaatcaa caaattgtca atgatgatgt tgtatttcgt
    40081 ttgaaagcgt tggcacaatc cacaatgcga actgctaaag tacaacaaca agtgcttgaa
    40141 gaagaaaaca accaatacga cttttaaagt ataaaaataa gacgctcggc acactaaatt
    40201 tgtatgtgtt tgagcgtctt attatattaa ctatatagcc agtgaacgac gataaaggca
    40261 agtgatgaca agcatattga ggtaataatg attgtcataa gcggtttaag tgcacgattt
    40321 ttaagatctt taaatgcaac atttaaccct aaagcaacca tggccattaa taagcaaatt
    40381 gttgatacag tatttaaaat atttagcaat gctgacggaa tagttacata tgtattcact
    40441 aaggccataa tgacaaatcc aattaaaaag tatggaatgc ttattcgacc cttgctagat
    40501 gattctgatg aacgaaaacg cataattaaa ataagtacga tggttaatgg aatcagtaag
    40561 aatactctac caagtttacc aagaagtgcg attttaagtg catcactacc accaaagcca
    40621 ccagctaaga caacgtgtgc aatttcatga agactaacgc cggaccaagc gccataaaca
    40681 tttgtcgtca ttgaaaagat agcgtagata gctgtatata taagtgaaaa tatcgtacca
    40741 atcaatgcga tgataccgat actaatagct gtatcctttt cacgtgattt gaatattggg
    40801 gctactgcag caatagcggc agcaccacaa acgcctgtac ctacacctag taataatgcg
    40861 atgtttttgt caccatgcaa cagtttgttg acaaagagca tcattacaat actgaaaata
    40921 acgacaccta catcgatggc taatagtcta ctaccttgac cgataatatc gaatatattg
    40981 agtttaagtc catataggat gattgcaaat cttaataaat atttagatga aaacgtaata
    41041 cctgagctat attgttcagg atatcctcta aagtgacgat atagaatagc gattaatatc
    41101 gcgatagtta atgcgccaac cttatctaag attggtaatt tagctgctaa aaagctaaat
    41161 aatgcgacta taaatgtaaa tgatagtcca atcataaaat gcttgttttt caatgatgcc
    41221 atgagcagtg cctcctttaa tagcatttta gcactgtttt gtcgtatttt taaatataaa
    41281 tttggaatga ataataaaat ggtatttaaa ttaagttgtg ttgtaggtat caaggacatc
    41341 aaccaaagta ataggcacta caacgcttat tggcggggcc ccaacaaaga gaatttcgaa
    41401 aagaaattct acagacaatg caagttgggg aaggacaaca aatttaagat acaaggcgca
    41461 gtattaatat gttattatta tgataattta cagaattata tgaaaaatga atgaggatgt
    41521 gatggtatgt ttggaatgaa agtgaatgaa caaataatat taaaaatttt agaagctcat
    41581 gacacagagg cacttttcaa tttagtcaat cgttcaagag attcacttag ggaatggtta
    41641 ccttgggtag atgcaactga gcagccatca gatacgcatg cgtttattaa aagaggactt
    41701 ttgcaatttg cggatggtaa tgggtttcag tgtggcattt ggtatgaagg aaggctagct
    41761 ggtgtagtcg gtttacatga aattaaccat atgcatagaa aaacatcatt aggctattat
    41821 ttggataaac aatttgaggg aaaaggagtt atgacacaag ccgtcgagac attgataaag
    41881 tattgcttcg aagagattga cttaaatcga attgaaataa gtgccgcagt taataatcgg
    41941 aaaagccaag cgattcctga aaggcttggg tttactaaag aaggaatgtt acgtgacaat
    42001 gaattactaa atggtattta ttcatcgagt tacatctata gtttattgaa atcagaatac
    42061 gaccaaaaat aacaaattag aattataaaa gagtgatgac aattaaaatg gcatcgctct
    42121 tttatttaat ttccaaaaat aaaaggttgt tgacagtatt attttataac aatataatga
    42181 ttttgataat tattatcaac tagatggtgt ttatgggagg atgctttaaa acagccgttt
    42241 taagtgtaat gtattatttt agcgtgtagg gaatgcgaaa ataatattta taagaacaca
    42301 tctatgtgga taatagaatt tctataatga ggtgtcaaaa tgaaaaagtt aacaacgcta
    42361 ttattagcat caacgttatt aattgctgca tgtgggaacg acgatagtaa gaaggatgat
    42421 tcaaagacat cgaaaaaaga tgatggtgtt aaagcagaat taaaacaagc aacaaaagca
    42481 tatgataaat atactgatga acagttgaat gaatttttaa aaggaacaga gaaatttgtt
    42541 aaagcaattg aaaataatga tatggctcaa gcaaaagcat tatatccaaa agtgcgtatg
    42601 tattatgaac gctctgaacc agttgccgaa gcatttggtg acttagatcc taaaattgat
    42661 gcgcgtctag cagatatgaa agaagagaaa aaagaaaaag aatggtcagg atatcataaa
    42721 attgaaaaag acttatatga agataacaaa attgatgata tgactaaaaa agatgcacaa
    42781 caattattaa aagatgcaaa agaattacat gcaaaagcag atactttaga tattacaccg
    42841 aaattaatgt tgcaaggttc tgttgaccta ttaaatgaag ttgcaacttc taaaattaca
    42901 ggtgaagaag aaatttactc gcatacagat ttatatgatt ttaaagcgaa cgttgaaggt
    42961 gcacaaaaaa tttatgattt attcaaacca atcctagaga aaaaagataa aaaattaagt
    43021 gacgatattc aaacaaactt cgataaagta aaccaacttt tagataaata taaagataag
    43081 aatggtggtt atgaatcatt tgaaaaagta tcgaaaaaag atcgtaaagc atttgcagat
    43141 gccgttaatg cattaggaga gccactaagt aaaatggctg tgattactga atgacaaatt
    43201 acgaacaagt taatgacagc acacaatttt caagacgtac atttttaaaa atgttaggta
    43261 ttggtggtgc tggtgttgca attggcgcga gtggtgtggg tagtgtttgg tctttcaaat
    43321 cgatgtttaa tacacctgaa gacccagaaa aggatgcata tgaatttcat ggcaaagttc
    43381 aaccaggaat aacaacgcca acgcaaaaaa catgtcactt tgtagcatta gatttgaagt
    43441 caaaagatcg agatgcgatt aagtcgatgt ttaaaaagtg gactgtgatg gctgatcgaa
    43501 tgatggatgg agatacggtt ggtaaagcaa gtaagaatcc gctaatgcca cctgtagaca
    43561 ctggtgaagc tatcggtctt ggtgcgagca aattgactct tacatttggt attagtaaat
    43621 cgatgatgaa gaaaatagga ttatccggta aaatccctga tactttcaaa gatataccgc
    43681 attttccaaa tgatcaatta atcgatgatt acagcgatgg ggacattatg attcaagctt
    43741 gttctaatga ttcgcaagtt tcatttcatg cagttcataa tttagttcgt ccctttagag
    43801 atgtcgttaa ggtgcgatgg gcacaatctg gcttcatatc tgctaaaggt aaggaaacgc
    43861 ctagaaattt gatggctttt aaagatggaa ctattaatcc tagaaaaagt aatcaactga
    43921 aggattatgt gttcattgat gatggatggg cgaaacatgg cacatattgt gttgtcagac
    43981 gtattcaaat acacattgag acatgggacc gtaccgcatt agaagaacaa gaggcgacat
    44041 ttggtcgtaa gcgtcatagt ggtgcgcctt taacaggtgg caaagagttt gatgaaattg
    44101 atttgaaagc gaaagatagt cacggagagt acattataga taaagatgcg catacgagac
    44161 tagcgaaaga agctaatacc tccattttgc gtagagcatt taattatgtt gatggtaccg
    44221 atgaacgaac tggtaacttt gagacaggct tactttttat tgcttttcaa aaagcgacaa
    44281 aacaatttat cgatatacaa aataatttag gtagtaatga taaattaaat gaatatatta
    44341 cacatagagg gtctgcttca tttttagtat taccaggtgt tagtaaggga ggataccttg
    44401 gtgaaacatt atttgattaa aattgtagcg acgataatta ctttagttat ggtatttagc
    44461 tttggattat tgaaaagtca gggagtagaa cagcatagta taagtgatgt atatagtgtg
    44521 ataacggatg ctaaatctgc actttcgagt aataaaatat ctaatgatag taaaaaagat
    44581 gcagttaaaa aagttgtaag tagtgtgggg aaactatcac ttgatgacaa cagtgaagga
    44641 aatgctgtta aatcagactt gagaaaactt aaagatgcta aatcgaatgc tgatcaaaca
    44701 gatgcattat cacaattgac gaagtcatta attgcctatg aagagaagtt ggctagtaaa
    44761 gatgcaggtt ctaaaattaa attattgcaa cagcaaatcg atggtaaaga tagagcgatg
    44821 acaaaagcga ttaaagataa aaataaagca gagctagaat cgctgaacaa tagtttgaat
    44881 cagatttgga caagtaatga aacggtgatt cgcaattatg acgcaaatca atatggacaa
    44941 attgaagtcg cattactgca acttagaatc gcaatccata agtcgccatt agatacagcg
    45001 aaagtgtcac atgcttggac aacttttaaa tcaaatattg atcatgtcga taagaaaagt
    45061 agtgcctctg aaagtaatca atttcatgta tcacagttga atgatgaatt agataaagcg
    45121 attaaagcaa tagatagcaa tcaattgtca gatgctgata cagcacttac acattttata
    45181 gaaatttggc catatgttga aggtcagatt caaactaaag acggtgcttt gtatacgaaa
    45241 attgaagata aaataccata ttatcaaagt gtattagacg aacataataa atcacacgtg
    45301 aaagatggtt tagtagatat aaacaatcaa attaaagagg ttgttggcca tagttacagc
    45361 tttgttgatg tgatgattat ctttttacgt gaagggctag aagtgttgtt aattgtaatg
    45421 acattgacta ccatgacgcg taatgtcaat gataagaaag gaactgcaag cgttattggt
    45481 ggtgccattg ccggacttgt actgagtatc atcttagcaa ttacgttcgt agaaacttta
    45541 ggtaatagtg gcattcttcg tgaaagtgtg gaagcgggat taggtattgt tgcggtcata
    45601 ttaatgttta tcgttggtgt ttggatgcac aaacgttcaa atgcaaaacg ttggaatgac
    45661 atgattaaaa atatgtatgc taatgcgatt agtaatggta atttggtatt gttagcgacg
    45721 attggtttaa tttctgtact ccgtgaagga gttgaggtaa tcattttcta tatgggaatg
    45781 ataggtgagc tagcgacaaa agattttatt attggtattg ctttagctat cgttatttta
    45841 atcatctttg cattattatt tagatttata gttaaattaa tacctatttt ctatatattt
    45901 agagtgttgt cgatttttat ttttattatg ggattcaaaa tgcttggcgt aagtattcaa
    45961 aagttacaat tattaggtgc tatgcctaga catgtcattg aaggattccc aacgattaac
    46021 tggttaggat tctatccaag ttatgaacca ttgatagcac aagtcattta tattattata
    46081 gtagctttct taatctttaa atttaaaaaa taaaaaacag gccgagtgcc tgtttttttg
    46141 ttgtgatgtt ggaaatattc ggtattgcag tataaccata atcacagcat taattcttat
    46201 aagattagtg tggcggtttg actcggcatg tgaacttaac aatgaacata ctgaactcaa
    46261 agagcaatat gagtggcaat gtaagtaata tatttaatgt taaatcgggt ggtgcaatga
    46321 tacttgctaa tacaaagcaa gcgaagtaaa tatattttcg ataatgcttc aatgatgtgg
    46381 tatctataag accgagtttt gtaagaccta tgaataatat tggcaattgg aataaaattc
    46441 caaaagtgaa aagccaacgg ataagttcaa ctaaatatgc tttaaagcca atcactggag
    46501 agatgttcaa agtggttgat aattttaaag cgaattgaat aatcattgga aagccaatat
    46561 aaaatgcaaa agcgacacca aaaataaata aaagtacgct aaacaagcta tatttataaa
    46621 taaattgacg ttcattatta tgtaatccag gcgcaatgaa tgcccacaat tgataaaaca
    46681 tgactggtga aatgaaacaa aacgcgatga aaaatataat catcacatat atttgaatca
    46741 tttcagtgaa tgaaaaggca tgtaaagtta catttgctcg agtaatgcat gatatgactg
    46801 atgtcatcca ccaaaatgat gaaacataaa cgatgatgac tgcgatgata aacgacaata
    46861 gtattttaac taaacgatga cgtagttcac taaagtgaac cattaacgtg tgatcagtgt
    46921 tattaatctc gctctcgttg ttgcttagat tccttactgg gtgtgtcgtg agactcttta
    46981 tctaaatctt ctgttgcaga tttaaattct tttaaagtag aaccgatagc acgaccaaat
    47041 tgtggtaatt ttttcggacc aaaaataatt aaagcgataa tgctaatgac gacaagactt
    47101 gttgggcctg tgatgcctaa aataaaagtg ttagttatca tgataatcaa cctcactcat
    47161 aagtagtata tagtatttac tttataccga acgataatga ttatcaacaa attttatata
    47221 ataattaaaa tattgctata aacacaactt actgaggtat taattctgaa atgatggctt
    47281 gttctttcaa gtcgatataa gtacaagtac cagcttgaat atcgatatgc catttataaa
    47341 tgccagcttc agccatttca tcacaaaatg tttcaaaatc tgtttgccct tgttgatgtc
    47401 ttgttaagac gtcttgaacc attgttttgt ttgatttttg agcaacagga atcgtacttt
    47461 tcacagatga agtaacgata tcatcttctg attgatgaac gtatgttgca gtgccatttt
    47521 gaatgttgac gatattgtaa gtcatcccca tatctttaaa agctttgaat agttttggaa
    47581 aatcaacacc agtaaattgt tgatgtgctt gttgaattgc agataatgta aatgccataa
    47641 taatctcctt tgaatgattt aatttagtga aatgcgctat taaattactg tttgaaaatg
    47701 cgtgcataac gtgcataata ctaaaattag aatgctgtta attatgaagc aagatttagt
    47761 gaaagtaaaa aaatacggct cgcaggtgtt gagaaattcg aatcataatt taataaattg
    47821 gtgaacggtg gtattttttt gtgctaattt gacaaatata gttgtcaaaa agacaaatat
    47881 catttacagt aggattaagg agttgatgcg atgtgcgtaa tcgattgaaa gagttacgag
    47941 cacgagatgg cttaaatcaa acgcagcttg ccaaactagc tggtgtttca agacaaacca
    48001 tttctctaat tgagcgaaac aattttatgc catcagtatt aacggcaata aagattgccc
    48061 gtatcttcaa tgaaacggtg gaaactattt ttattattga ggaggatgag gcatgaaaat
    48121 actaagatat attggatatc ttttactggg tggacttgta ggaggtatca taggtggaat
    48181 tttaggtaac tttgatggat tgggtattga gaacttgacg tttgcgacat ataacaatgt
    48241 cgttgtaata tctatagttg cgacgattat tatcatattg gtagaaggca ttattttgat
    48301 gaatcaaaga cgtgcattga agtataaacg acttgtagat gaagaagtag atatcgatgc
    48361 aacagatcaa tatgaattgc ttgcgaatcg ttatgtttta aatgggagta tattaagtat
    48421 tctgcagaca gttattgctt ttttagtatt gcttattttt gtggtagggc acgctgcagc
    48481 aaatgcaatg ctattctttt taataccatt ttttgctagt gctattttca atacacaatt
    48541 tacgctgttt aatagaagat ttgatgatag aatgccaaaa attgcagata agaattacac
    48601 tgaaaagcgt ttggaaatat tggatgaagg tgaacgacac atagaattag tggcattgtt
    48661 taaaacttat gcgatcaact tatcgttatt aatactagca attattttta tagggtctta
    48721 ttcaattgct acaggaatta atcaaagctt cagtttacta cttatcattg ctattttcat
    48781 atataacgcc tttagttatt tattgaagag aagacgtttt tattaaaatt aaacaaagag
    48841 gagcatggat atgacaacat tgttaaacgt agatcgtgtg aacaaacaat acaaagattc
    48901 ggattttaaa ctacaagatg catctttaac gatttctact aatgagacag taggattaat
    48961 cgggaaaaat ggctcgggta aatcgacatt aattaatatt ctagtaggca atcgacataa
    49021 agataacggt agtattgcat tttttggaga agaacatgct gcggatgatg tcgaatataa
    49081 agaacatata ggtgttgtgt ttgatgattt gagagtacct aataaattga ctattaaaga
    49141 tatcgataaa gtatttcaaa atatttatac gacttggaat agtcaaaaat tctttgattt
    49201 aatcaaatat ttcgagttgc cacaacaaac taaaattaaa actttttcac gaggaatgag
    49261 aatgaagata gctttaacga ttgcgctttc tcatgatgtg aagttattaa tcttagatga
    49321 agcaactgca ggtatggatg tttctggcag agaagaagta atggaaatac tagaagattt
    49381 tgtcgcacaa ggtggaggca tcttaatatc atcgcatatt tctgaagata ttgaacattt
    49441 agcggataaa ttagtgttta tgaaagatgg aaaaattatt ttaactgaac agaaagatat
    49501 actgttagca caatatggaa tcgtaacgac ggaagataaa gatgtcgaaa ttccaaatca
    49561 tttaatgatt gcttctagat tatcgaaggg gaaatatcaa attttagtta aagattatgc
    49621 agaaattgaa aatgcagaac ctttaaaaca cattgatgac gctacgaaaa tcataatgcg
    49681 aggtgaagta taatgaaagg tatgttccta agtagttttt atgcaacgag aaagcaaaca
    49741 tatatttatt ttatagtcgc tattatagct gcaggatact ttgcagtatt taatccgttg
    49801 atgagttcgg caatggctgg ggttatgtta atcacaccta ttactgataa tattaaacat
    49861 gaaaaagact caagatggat gtattatgta tctactttac cggttaaacg tagtgattat
    49921 attaagtcat actttgcctt ttatttaatc ttattcggtg caagtttaat gattggatta
    49981 gttgtgacta cgattgtgac ccaaagtgtg atgattggta ttatgtcagg tttaatgagt
    50041 tttggtatca taggggcata ctctatcatt ttcccattga catttaaatt tggcgctgaa
    50101 aactccaatg tcattatgat atgtgcatct atactactac ttatttcttt cgtggtcttt
    50161 ttctttatat atggtatggt tagtggtgca tctgcattag aatttgaaaa aattagcact
    50221 gaaggatggc tagttgtcat agtatatgcg gttatcggta tagttataac aagtgtttct
    50281 tatatattgt ctattaaaat tttt
//
LOCUS       DAHHRR010000004        49862 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.004, whole genome shotgun sequence.
ACCESSION   DAHHRR010000004 DAHHRR010000000
VERSION     DAHHRR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 49862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49862
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.004"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            131..1018
                     /gene="pdxS"
                     /locus_tag="NBI42_000187"
     CDS             131..1018
                     /gene="pdxS"
                     /locus_tag="NBI42_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HCD1776653.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKENGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASDLGTAM
                     KGLDINQLSLEERMQERGW"
     gene            1022..1612
                     /gene="pdxT"
                     /locus_tag="NBI42_000188"
     CDS             1022..1612
                     /gene="pdxT"
                     /locus_tag="NBI42_000188"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCD1776654.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKHSLMYAKSTNY"
     gene            complement(1789..3003)
                     /locus_tag="NBI42_000189"
     CDS             complement(1789..3003)
                     /locus_tag="NBI42_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD1776655.1"
                     /translation="MHTLIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            3161..3622
                     /locus_tag="NBI42_000190"
     CDS             3161..3622
                     /locus_tag="NBI42_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCD1776656.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            3641..4207
                     /locus_tag="NBI42_000191"
     CDS             3641..4207
                     /locus_tag="NBI42_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCD1776657.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            4197..5204
                     /locus_tag="NBI42_000192"
     CDS             4197..5204
                     /locus_tag="NBI42_000192"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCD1776658.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKLKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            5218..7674
                     /locus_tag="NBI42_000193"
     CDS             5218..7674
                     /locus_tag="NBI42_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCD1776659.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            8154..9518
                     /gene="radA"
                     /locus_tag="NBI42_000194"
     CDS             8154..9518
                     /gene="radA"
                     /locus_tag="NBI42_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCD1776660.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSF
                     ALHS"
     gene            9543..10616
                     /locus_tag="NBI42_000195"
     CDS             9543..10616
                     /locus_tag="NBI42_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCD1776661.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKSHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            11174..12628
                     /locus_tag="NBI42_000196"
     CDS             11174..12628
                     /locus_tag="NBI42_000196"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCD1776662.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMEISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            13052..13699
                     /gene="cysE"
                     /locus_tag="NBI42_000197"
     CDS             13052..13699
                     /gene="cysE"
                     /locus_tag="NBI42_000197"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCD1776663.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            13683..15083
                     /locus_tag="NBI42_000198"
     CDS             13683..15083
                     /locus_tag="NBI42_000198"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCD1776664.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            15076..15480
                     /locus_tag="NBI42_000199"
     CDS             15076..15480
                     /locus_tag="NBI42_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCD1776665.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEVLLNKIITIVNER"
     gene            15488..16234
                     /gene="rlmB"
                     /locus_tag="NBI42_000200"
     CDS             15488..16234
                     /gene="rlmB"
                     /locus_tag="NBI42_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCD1776666.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLSTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDDLKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            16234..16758
                     /locus_tag="NBI42_000201"
     CDS             16234..16758
                     /locus_tag="NBI42_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCD1776667.1"
                     /translation="MKERYLIIDGYNMIGQSQTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            16839..17408
                     /locus_tag="NBI42_000202"
     CDS             16839..17408
                     /locus_tag="NBI42_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCD1776668.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRYDVENNYLNKLAIKELIRQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            17523..17666
                     /gene="rpmG"
                     /locus_tag="NBI42_000203"
     CDS             17523..17666
                     /gene="rpmG"
                     /locus_tag="NBI42_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD1776669.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            17722..17904
                     /gene="secE"
                     /locus_tag="NBI42_000204"
     CDS             17722..17904
                     /gene="secE"
                     /locus_tag="NBI42_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCD1776670.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            17917..18465
                     /gene="nusG"
                     /locus_tag="NBI42_000205"
     CDS             17917..18465
                     /gene="nusG"
                     /locus_tag="NBI42_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCD1776671.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            18646..19068
                     /gene="rplK"
                     /locus_tag="NBI42_000206"
     CDS             18646..19068
                     /gene="rplK"
                     /locus_tag="NBI42_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCD1776672.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            19276..19968
                     /gene="rplA"
                     /locus_tag="NBI42_000207"
     CDS             19276..19968
                     /gene="rplA"
                     /locus_tag="NBI42_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCD1776673.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     misc_feature    20056..20202
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            20240..20740
                     /locus_tag="NBI42_000208"
     CDS             20240..20740
                     /locus_tag="NBI42_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCD1776674.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     gene            20783..21151
                     /gene="rplL"
                     /locus_tag="NBI42_000209"
     CDS             20783..21151
                     /gene="rplL"
                     /locus_tag="NBI42_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCD1776675.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            21326..21934
                     /locus_tag="NBI42_000210"
     CDS             21326..21934
                     /locus_tag="NBI42_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1776676.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLKHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKTHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            22149..25700
                     /gene="rpoB"
                     /locus_tag="NBI42_000211"
     CDS             22149..25700
                     /gene="rpoB"
                     /locus_tag="NBI42_000211"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCD1776677.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            25864..29460
                     /gene="rpoC"
                     /locus_tag="NBI42_000212"
     CDS             25864..29460
                     /gene="rpoC"
                     /locus_tag="NBI42_000212"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCD1776678.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEVI
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            29597..29851
                     /locus_tag="NBI42_000213"
     CDS             29597..29851
                     /locus_tag="NBI42_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCD1776679.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            29949..30362
                     /gene="rpsL"
                     /locus_tag="NBI42_000214"
     CDS             29949..30362
                     /gene="rpsL"
                     /locus_tag="NBI42_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCD1776680.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            30428..30898
                     /gene="rpsG"
                     /locus_tag="NBI42_000215"
     CDS             30428..30898
                     /gene="rpsG"
                     /locus_tag="NBI42_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCD1776681.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            31021..33102
                     /gene="fusA"
                     /locus_tag="NBI42_000216"
     CDS             31021..33102
                     /gene="fusA"
                     /locus_tag="NBI42_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCD1776682.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            33319..34503
                     /gene="tuf"
                     /locus_tag="NBI42_000217"
     CDS             33319..34503
                     /gene="tuf"
                     /locus_tag="NBI42_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCD1776683.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(34785..35960)
                     /locus_tag="NBI42_000218"
     CDS             complement(34785..35960)
                     /locus_tag="NBI42_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1776684.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGHNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLEDVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTIKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVIDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDIHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            36131..37318
                     /locus_tag="NBI42_000219"
     CDS             36131..37318
                     /locus_tag="NBI42_000219"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCD1776685.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEQTLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMASTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            37584..38462
                     /gene="hchA"
                     /locus_tag="NBI42_000220"
     CDS             37584..38462
                     /gene="hchA"
                     /locus_tag="NBI42_000220"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCD1776686.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDDAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            38620..40257
                     /locus_tag="NBI42_000221"
     CDS             38620..40257
                     /locus_tag="NBI42_000221"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCD1776687.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPVNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAANRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPETE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQCLRVEG"
     gene            40471..41436
                     /locus_tag="NBI42_000222"
     CDS             40471..41436
                     /locus_tag="NBI42_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCD1776688.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            41772..42848
                     /locus_tag="NBI42_000223"
     CDS             41772..42848
                     /locus_tag="NBI42_000223"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCD1776689.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPFGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            43099..43782
                     /locus_tag="NBI42_000224"
     CDS             43099..43782
                     /locus_tag="NBI42_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1776690.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEKYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADIIEALN"
     gene            complement(43898..44560)
                     /locus_tag="NBI42_000225"
     CDS             complement(43898..44560)
                     /locus_tag="NBI42_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD1776691.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPDSLNPMIDKIARIIKTYRQVD
                     TR"
     gene            complement(44553..45170)
                     /locus_tag="NBI42_000226"
     CDS             complement(44553..45170)
                     /locus_tag="NBI42_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD1776692.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYRKY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            45237..45707
                     /locus_tag="NBI42_000227"
     CDS             45237..45707
                     /locus_tag="NBI42_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCD1776693.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            45854..46723
                     /locus_tag="NBI42_000228"
     CDS             45854..46723
                     /locus_tag="NBI42_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002477520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1776694.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIRNGIQRRIDNGTLKVVDNYDAI
                     EDIPGELIMKILAFDADLEKIDKASKILAESPNLAVSSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKDVMAIGDNLNDVSMLAKAGYPVAMENGADEVKEIAKFITDTNENSGV
                     GKAIMKLLREQQD"
     gene            46743..47309
                     /locus_tag="NBI42_000229"
     CDS             46743..47309
                     /locus_tag="NBI42_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD1776695.1"
                     /translation="MKGLIIIGSAQVNSHTNALATYLTEHFKTHNIEAEIFDLAENPL
                     NQLDFSGTTPSVDEIKQNIKNLKDKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
     gene            47740..>49862
                     /locus_tag="NBI42_000230"
     CDS             47740..>49862
                     /locus_tag="NBI42_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HCD1776696.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTVPTENKTTEKVDSSQQNNIEQSTTSNQPKVNESDNT
                     SVKETTEEPQNTTSTQPTKQNNDATANKDNLAAQNISTQANDVSATPKTTTIKPRTLN
                     RMAVNTVAAPQQGTNVNDKVHFKDIDIAIDKGHVNQTTGKTEFWATSSDVLKLKANYT
                     IDDSVKEGDTFTFKYGQYFRPGSVRLPSKTQNLYNAQGNIIAKGIYDSETNSTTYTFT
                     NYVDQYTNISGSIEQVSFAKRENATTDKTAYKMEVTLGNDSYSKEVIVDYGNKKAQPL
                     ISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFKIYEVTDQNQ
                     FVDSFTPDTSKLKDVTGQFDVIYSNDNKTATVDLMKGQTSSNKQYIIQQVAYPDNSST
                     DNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTNKDGKQDANE
                     KGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGYTPTTANAGT
                     DDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKG
                     VKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGTNTTEDDKDA
                     DGGEVDVTITDHDDFTLDNGYYEEET"
BASE COUNT        18081 a         7207 c         9594 g        14980 t
ORIGIN      
        1 aagttgatgg tataaagtgt ttttcatttt taaaactgac ctcctaattt tatattattt
       61 tgcacctttt taaatatcag tttatacatt acaatgaatt taatcaactt gaaaaggggt
      121 tttatataaa atgagtaaaa taattggatc agacagagtc aaaagaggta tggccgaaat
      181 gcaaaaaggc ggcgttatca tggatgtcgt taatgctgag caagcaagaa ttgcggaaga
      241 agcaggtgcg gtagcagtca tggctttaga gcgtgtacct tctgatatta gagcagctgg
      301 tggcgttgca cgtatggcaa accctaaaat tgtagaagaa gtaatgaatg ctgtttcaat
      361 tcctgtaatg gctaaagcac gtattggtca tattacagaa gcaagagtat tagaagctat
      421 gggtgttgac tatattgatg aatcagaagt attaacacca gcagatgagg aatatcactt
      481 aagaaaagat caatttacag tgccatttgt atgtggttgt cgtaatttag gtgaagctgc
      541 acgaagaatc ggtgaaggtg ctgctatgtt acgtactaaa ggtgaaccag gtacaggtaa
      601 tattgttgaa gctgtaagac atatgagaca agttaattct gaagttagtc gattgactgt
      661 aatgaatgat gatgagatta tgacttttgc gaaagatatc ggtgcgcctt atgaaatttt
      721 aaaacaaatt aaagaaaatg gtcgtttacc ggtagttaac tttgcagctg gtggcgttgc
      781 gactcctcaa gatgctgctt taatgatgga attaggtgcc gacggtgtat tcgttggatc
      841 aggtattttt aaatcagaag atccagaaaa atttgctaaa gcaattgttc aagcaacaac
      901 acattaccaa gactatgaac taattggaag attagcaagt gaccttggca ctgctatgaa
      961 aggtttagat atcaatcaat tatcattaga agaacgtatg caagagcgtg gttggtaaga
     1021 tatgaaaata ggtgtattag cattacaagg tgcagtacgt gaacatatta gacatattga
     1081 attaagtggt catgaaggta ttgcagttaa aaaagttgaa caattagaag aaatcgaggg
     1141 cttaatatta cctggtggcg agtctacaac gttacgtcga ttaatgaatt tatatggatt
     1201 taaagaggct ttacaaaatt caactttacc tatgtttggt acatgcgcag gattaatagt
     1261 tctagcgcaa gatatagttg gtgaagaagg ataccttaac aagttgaata ttactgtaca
     1321 acgaaactca ttcggtagac aagttgacag ctttgaaaca gaattagata ttaaaggtat
     1381 cgctacagat attgaaggtg tctttataag agcgccacat attgaaaaag taggtcaagg
     1441 cgtagatatc ctatgtaagg ttaatgagaa aattgtagct gtccagcaag gtaaatattt
     1501 aggcgtatca ttccatcctg aattaacaga tgactataga gtaactgatt actttattaa
     1561 tcatattgta aaaaagcata gcttaatgta tgctaaatca acgaattatt gatatttata
     1621 gatttgttga gaagacaata tctccttcaa acttagcttt ggaggagtta ttttttatgt
     1681 caaaattaaa aatgatagaa aataaagata tacataagaa aaaacccctt caaagagact
     1741 gagaatagtc aaaattttga aggggttaat tcgatgttga tgtatttgtt aaataaagaa
     1801 tccagcgatt gcagctgaaa tgaaagatac tagtgttgca ccgaataata atttcaaacc
     1861 aaagcgggca actgtatctc cttttttgtc attaagtgat ttaatcgcac ctgaaataat
     1921 accaatagag ctaaagttag caaatgatac taagaataca gatgtaacac cttttgcgtg
     1981 ttcagataaa tcactaagtt taccaagtgc ttgcattgct acaaattcgt tagataatag
     2041 ttttgtcgcc ataactgaac cggcttgaac tgcatcttgc catggcacac cgactaagaa
     2101 tgcaaatggt gcaaagacaa aaccaattaa tgtttggaaa tcccaagaaa tagcgccacc
     2161 tgaaactgta ctaaagatat tgcttacaat tccatttaat agagcgataa tggcaatgta
     2221 tccgattaac attgcgccta caatgacagc tactttaaat ccatctaaaa tatattctcc
     2281 tagcatttcg aagaatgatt gttgtctttc ttcagtttct tcaactaata atttgtcatc
     2341 ttcttcatta actttataag ggttaataat tgaagcgatg atgaaaccac caaataagtt
     2401 taagacaaca gccgttacaa catatttagg ttcaattaag gtaaagtatg caccgataat
     2461 tgaagcagaa acagtcgaca ttgctgaagc tgttaatgtg tataaacgtt gcttaggtat
     2521 gtatggtaat tgttttttaa ttgaaataaa tacttcagat tgtcccaaaa ttgctgcagc
     2581 aactgcattg tatgattcta aacgtcccat accattaatt ttagaaatta agaatcctaa
     2641 aacattaatg attaaaggta aaatctttgt gtattgaagg ataccgataa tcgctgaaat
     2701 aaatacgata ggtaataata cactgaagaa gaatggtggt tgcttaggat cgatatattg
     2761 aataccaccg aatacaaagt taacaccatc tgcagctttt aataataagt agttaaaacc
     2821 gtttgaaata ccaccaataa ccttgattcc cattgtagtt ttaagcaaga taaatgcaaa
     2881 gataagctga attgcaagta aaattcctac atatttccag cgaatatttt tcctgtctga
     2941 gctaaataga aacgcaagtg ctaaaaagaa gataattccg ataatcccaa ttagagtatg
     3001 catatatttc tcattccttt agttttttct acaatctatc atacaataaa atggaagggc
     3061 taacatcata aatttttgaa aatataaaaa caaattaatt gaaaaaggtc aaaataggtc
     3121 atataatata gtcaaagaag gtcaaaaagg ggtgatatac atgcacaata tgtctgacat
     3181 catagaacaa tacatcaaac gtttatttga agagtcgaat gaggatgtcg ttgaaattca
     3241 gagagcgaat atcgcgcagc gttttgattg cgtaccatca caattaaact atgtaatcaa
     3301 aacacgattc actaatgaac atggttatga aatcgaaagt aaacgtggtg gtggtggtta
     3361 catccgaatc actaagattg aaaataaaga tgcaacaggt tatattaatc atttgcttca
     3421 gctgattgga ccttctattt ctcaacaaca agcttattat attattgatg ggcttttaga
     3481 taaaatgtta ataaatgaac gtgaagctaa aatgattcaa gcagttattg atagagaaac
     3541 gctatcaatg gatatggttt ctagagatat tattagagca aatattttaa aacgtttgtt
     3601 accagttata aattattact aaatgaaatg aggtgttgaa gtgctttgtg aaaattgtca
     3661 acttaatgaa gcggaattaa aagttaaagt tacaagtaaa aataaaacag aagaaaaaat
     3721 ggtgtgtcaa acttgtgctg aggggcacca tccgtggaat caagctaatg aacaacctga
     3781 atatcaagaa catcaagata attttgaaga agcatttgtt gttaagcaaa ttttacaaca
     3841 tttagctacg aaacatggca ttaattttca agaagtagcg tttaaagaag aaaaacgttg
     3901 cccatcatgt catatgactt tgaaagatat tgcacatgtt ggtaaatttg ggtgtgctaa
     3961 ttgttatgca acatttaaag atgacatcat tgatatcgtc cgcagagttc aaggtggaca
     4021 atttgagcac gtcggaaaga caccacattc ttcacataaa aagatagctt taaagcgaaa
     4081 aatcgaagaa aagaatgaat atttgaaaaa acttattgaa atccaagatt ttgaggaagc
     4141 agccattgtt agagatgaaa ttaaagcact aaaagctgag agtgaggtgc aacatgatga
     4201 cgcataatat tcatgataat atcagccaat ggatgaaaag taatgaagaa acacccattg
     4261 ttatgtcttc tagaattcgg ttagcgcgta atttagaaaa tcatgtgcat ccactaatgt
     4321 atgctactga aaatgatgga tttagagtta taaatgaggt acaagatgcc ttgccaaatt
     4381 ttgaattaat gcgtcttgat caaatggatc aacaaagtaa actgaaaatg gttgcaaagc
     4441 atttgattag tcctgaacta ataaaacaac cagcagccgc agtattagtg aatgatgatg
     4501 aatctttaag tgtcatgata aatgaagagg atcatattcg tattcaagct atgggaactg
     4561 acacgacatt acaggcttta tataatcaag cttcatcaat tgatgatgaa ttagatcgaa
     4621 gccttgatat aagttatgat gaacaacttg gttatttaac tacatgtcct accaatatag
     4681 gtactggtat gagagcaagc gtgatgctac atttaccagg tctatctatt atgaaaagaa
     4741 tgacacggat tgctcaaacc attaatcgtt ttggatatac aatcagaggt atttacggtg
     4801 aaggttcgca agtttatgga catacttatc aagtatccaa ccaacttaca cttggtaaat
     4861 ctgagttaga aatcatagaa acattaacag aagttgttaa tcaaatcatt catgaagaaa
     4921 aacaaatacg acaaaagtta gacacttata atcaattaga aacacaagac cgtgtttttc
     4981 gctcgctagg tattttacaa aactgtagaa tgataactat ggaagaggct tcttatagat
     5041 taagcgaagt taaacttggt atagatttaa attacattga attacaaaac tttaaattta
     5101 atgaattgat ggtagctata cagtcaccat ttttattaga tgaagaagat gacaaatctg
     5161 taaaagaaaa acgagcagat atactaagag aacatataaa gtaggaggtc attatttatg
     5221 ttatttggta gattaactga acgtgcacag cgcgtattag cacatgcaca agaagaagca
     5281 attcgtttaa atcattcaaa tataggaaca gaacacctat tattggggtt aatgaaagaa
     5341 cctgaaggaa ttgctgcaaa agtattagaa agttttaata tcactgaaga taaagtaatt
     5401 gaagaagttg aaaaattaat tggacatggt caagatcatg ttggtacatt gcattataca
     5461 cctagagcta aaaaagtcat tgaattatcg atggatgaag ctagaaaatt acatcacaat
     5521 tttgttggaa cggaacatat tttattaggc ttgattcgtg aaaatgaagg tgttgcagca
     5581 agagtttttg caaatctaga tttaaatatt actaaagcac gtgcacaagt tgtgaaagct
     5641 ttaggaaacc ctgaaatgag taataaaaat gcacaagcta gtaagtcaaa taatactcca
     5701 actttagata gtttagctcg tgacttaaca gtcattgcca aagacggtac attagatcct
     5761 gttataggac gtgataaaga aattacacgt gtaattgaag tgttaagtag acgtacgaaa
     5821 aacaatcctg tgcttattgg agagccaggc gttggtaaaa ctgctattgc tgaaggttta
     5881 gcgcaagcca tagtgaataa tgaggtacca gagacattaa aagataagcg tgttatgtct
     5941 ttagatatgg gaacagtagt tgcaggtact aaatatcgtg gtgaatttga agagcgtctg
     6001 aaaaaggtta tggaagaaat ccaacaagca ggtaatgtca tcctatttat tgatgagttg
     6061 catactttag ttggtgctgg tggtgctgaa ggtgctatcg atgcttcgaa tattttgaaa
     6121 ccggcattag cacgtggtga attacaatgt attggtgcta ctacattaga tgaatatcgc
     6181 aaaaatattg aaaaagacgc ggctttagaa cgtcgtttcc aacctgtaca agttgatgaa
     6241 ccttcagtag tagatacagt tgctatttta aaaggattaa gagatcgtta cgaagcacac
     6301 catcgtatta atatttcaga cgaagctatt gaagcggctg ttaaattaag taacagatac
     6361 gtttcagatc gtttcttacc agataaagca attgatttaa ttgatgaagc aagttctaaa
     6421 gtaagactta agagtcatac gacacctaat aatttaaaag aaattgaaca agaaattgaa
     6481 aaagttaaaa atgaaaaaga tgccgcagta catgctcaag agtttgaaaa tgctgctaat
     6541 ttacgtgata aacaaacaaa acttgagaaa caatatgaag aagctaaaaa tgaatggaaa
     6601 aatgcgcaaa atggcatgtc tacgtcttta tcagaagaag atattgctga agtcattgca
     6661 ggatggacag gtatcccatt aactaaaatc aatgaaacag aatctgaaaa acttcttagt
     6721 cttgaagata cgttacatga gagagttatt ggtcaaaaag atgcggttaa ttctattagt
     6781 aaggcagtta gacgtgcacg tgcaggattg aaagatccga aacgtccaat tggtagtttt
     6841 attttccttg gtccaactgg tgttggtaaa acagaattag ctcgagcttt agctgaatca
     6901 atgtttggtg atgatgatgc aatgattcgt gttgatatga gtgaatttat ggaaaaacac
     6961 gctgtgagtc gtttagtcgg tgcacctcca ggatatgtag gacatgatga tggtggacag
     7021 ttaactgaaa aagttagacg taaaccatat tctgtaatct tattcgatga aattgaaaaa
     7081 gcacatcctg atgtatttaa tattttatta caagttcttg atgatgggca tttaacagat
     7141 acgaaaggtc gtacagttga ttttagaaat acgattataa ttatgacttc aaatgttggt
     7201 gctcaagaat tacaagatca acgctttgca ggattcggtg gatcaagtga tggacaagac
     7261 tatgaaacaa ttcgaaaaac gatgttaaaa gaattaaaaa attcattccg tccagaattt
     7321 ttaaaccgtg tagatgatat cattgtattc cataaactaa caaaagaaga attaaaagaa
     7381 attgtaacaa tgatggttaa taaattaaca aatcgattat ccgaacaaaa tataaatatt
     7441 attgttactg ataaagcgaa agacaaaatc gcagaagaag gatatgatcc agaatatggt
     7501 gcaagaccat taattagagc gatacaaaaa actatcgaag ataatttaag tgaattaata
     7561 ttagatggta atcaaattga aggtaagaaa gttacagtag atcatgatgg taaagagttt
     7621 aaatatgaca ttgctgaaca aacttcagaa actaaaacac catcgcaagc ataattataa
     7681 aacagtccaa aacaaattaa agttttgggc tgttttttta gtagcattgt agaaattcgt
     7741 gaaagtatcc atcaacgaaa caatctaata aaataatcat caaaggatag ttaagaatta
     7801 tatgaaacaa gttaatgagc ctacagcgat agcataaagg tatgaatttt tataaagttt
     7861 ttttgtttga agatactagc aacttacgtc aaaataaaat aggtctatat aatattgtga
     7921 taaatctaga gaaaagagtt tactaagaaa ttattaagat tttatctttg aaagataccg
     7981 actatcaaca tgatgtaagt ttattttata atattcataa aaaataaatc tgataaaaca
     8041 gtttgcgtta gtagtcatgt taaaatacaa acaaatgtaa ctcacattta atttgtcata
     8101 atgggaatgt gcgtttaaaa tagatttgtt caaaggaaag tggaggtgca attttggcca
     8161 agaaaaaagt gatttttgaa tgtatggctt gtggttatca atctcctaaa tggatgggga
     8221 aatgtcctaa ttgtggcgct tggaatcaaa tggaggaaat tgttgaaaaa gcagccaatc
     8281 ctaaacatgg agtgaaaacc aaggaattag caggtaaagt acaaaaatta aatagtatta
     8341 aacatgaaac aacgccgaga gtgttaacag attcagcaga attcaaccgt gtattaggtg
     8401 gaggtattgt gagcggatcg ttagtactta ttggtgggga tccaggtatt ggtaagtcaa
     8461 cgttactttt acaaatttgt gcatcgttat ctcaaaagaa aaaagtacta tatattactg
     8521 gagaagaatc gcttagtcag actaaattac gtgcagagcg attagatgaa gattcaagtg
     8581 aattgcaagt attagctgaa acagatcttg aagttattta tcaaacagta aaagaagaac
     8641 aacctgattt attagtagtg gattcgattc aaacaatata tcatcctgaa atcagctctg
     8701 cgccaggttc tgtttcacaa gttcgtgaaa gtacacaaag tttaatgaat attgctaaac
     8761 aaatgaacat tgcaactttt atagtgggtc atgtaacgaa agaaggtcaa attgctggcc
     8821 caagattgct agaacacatg gttgatactg tgctttattt tgaaggcgat gaacaccacg
     8881 catatcgaat tttgcgagct gttaaaaacc gttttggttc aacgaatgaa atgggaatct
     8941 tcgaaatgaa gcaaagtgga ttaaaaggtg taaataatcc atctgaaatg tttttagaag
     9001 aacgttcaac aaatgttcca ggttcaacaa ttgttgcaac catggaggga accagaccac
     9061 ttttaataga agttcaagcg ctggtaactc caacgacttt taacaatccg agacgaatgg
     9121 caacagggat tgatcataat cgattaagtt tgttgatggc tgttttggaa aagaaagaaa
     9181 attatctatt acaacaacaa gatgcttata tcaaagtagc tggcggtgta aagttaacgg
     9241 agccagcagt tgatttaagt gtaattgtag caactgcgtc tagctttaaa gataaagctg
     9301 tcgacggatt agattgctat attggagaag ttggtttaac gggtgaggta cgtcgtgtat
     9361 ctcggataga acaacgtgtg caagaggctg caaaactagg tttcaaacgt gtaattattc
     9421 ctaaaaataa tataggcgga tggacatatc ctgaaggtat acaagtaata ggtgtaacta
     9481 ctgtacatga agcattgtca tttgctcttc attcataaaa catcaagaaa ggaggacatt
     9541 gtgtgaatat cgttaaacta atggttatta ttatttactt aattattggg agcgcattag
     9601 gaataattat tattcctgaa attgcaaatg atcttggatt acaaaactcc agctttttaa
     9661 aaaatcacta tgtagatggc attatcggta gtatttttat gttcttaatt tttggtgtat
     9721 ttattagacg agttactaac gctataaaag gtttagaaca ttttattatg cgtagaagtg
     9781 ctgttgaaat actattcgca acaataggtt taataatcgg attacttatt tctgttatgg
     9841 tgtcgtttat attagaatca attggcaact ctattttcaa tcatttcatt cctgtcataa
     9901 ttacgatatt actatgttat ttcggtttcc aatttggcct taaaaaacga gatgaaatgt
     9961 taatgttttt acctgagaat atagcgcgtt ccatgtcaca acatactaaa agtgctacac
    10021 caaaaattat cgacacaagc gcaattattg atggtcgtat tttagaagtc attcgttgcg
    10081 gttttatcga tggcaatatt ttaattccac aaggtgttat taatgaatta caaattgttg
    10141 cagattcaaa tgacagtgtt aaacgtgaaa agggtaaaag aggcttagat attttaaatg
    10201 aattatatga tttagattat cctacaaaag tcatacatcc aactaaatca catagtgata
    10261 ttgatacgat gttattaaag ctagctaaac aatatcatgc aagcattatt acaacagatt
    10321 ttaatttgaa taaagtttgt catgtacatg gcataaaagc attaaatgtt aatgatttat
    10381 cagaagcaat taaaccaaat gtacatcaag gagatcagct acatatttta ttaacaaaga
    10441 tgggcaaaga gcctggtcaa gctgttggat atttggatga tggtacgatg gttgttgttg
    10501 ataatgctaa aaatctaatt gggagtcatg tcaatttaga agtagtcagc ttattacaaa
    10561 catcttcagg aagaattgtt tttgctaaaa aaatcgaaga tacagtatca ttataaaaac
    10621 tgtagttgac gaaaacaaac atagatatta gaaggtatat gaaatggcga agtttgtaat
    10681 ttgaagtata actataaata aatgcaaaac atatcagtta tcaaaaaata caaatagtgc
    10741 taatatatca ttgaaacaag ttcattaagt gttaaatttt agatattaat aaagttattc
    10801 ttatagcaat caagacatac aaaattagaa tcaattttaa cgtatttgag tagttaattt
    10861 gaagtttaat tttacagcac ataatattca tttttaagtc gttgaaatgg gtatgtagtg
    10921 cagtcttgat tcttagggat agctttttta aagtgttgaa aaaacaactg ctttctattt
    10981 aaaatttaag catattaccc agggaattat gttatgatta aacaatgaaa ttattttaag
    11041 gtcatgttaa aaagcattgt ttcgttttaa caataagacg agttacacat ttgttatgac
    11101 ggtcgaagat agcaaatgaa caatttatat gaactaactt gaaaatttga tatttaattt
    11161 aggagtgaaa aaaatgagcg atcgtataag agtaagatat gcaccaagtc caactggtta
    11221 tcttcatatt ggtaatgcaa gaacagcatt attcaattac ttgtatgcta aacattacaa
    11281 cggagatttt gtgattcgaa ttgaagatac tgataaaaaa cgtaatttag aagatggaga
    11341 aacatcacaa tttgataatc ttaaatggtt aggattagat tgggatgaat ctgtagataa
    11401 agacaatggc tacggaccat atcgtcaatc tgaacgtcaa catatctacc aaccattaat
    11461 agatcagtta ctagcagaag ataaagcata taaatgctat atgacagaag aagaattaga
    11521 agctgaacgt gaagcgcaaa tcgctcgtgg tgaaatgcct cgctatggtg gtcaacatgc
    11581 gcatttgact gaagaacaac gtcaacaatt tgaagcagaa ggacgccaac catcaattcg
    11641 tttccgagta cctcaaaacc aaacgtattc atttgatgat atggtaaaag ggaatatttc
    11701 atttgattca aatggtattg gtgactgggt tatcgttaaa aaagatggca ttccaacgta
    11761 caattttgca gtagctatag atgatcatta catggaaatt tcagatgtta ttcgtggtga
    11821 tgatcatatt tcaaatacac ctaaacaaat tatgatttat gaagcatttg gctgggagcc
    11881 acctcgtttt ggtcatatgt cattaattgt taatgaagaa cgtaaaaagt taagtaaacg
    11941 tgatggacaa attttacaat ttattgagca atatcgtgac ttaggttatt tacctgaagc
    12001 gttatttaat tttattgcgt tattaggttg gtctcctgaa ggtgaagaag aaatcttttc
    12061 taaagaagaa tttatcaaaa tctttgatga aaagcgtttg tcaaaatcac cagcattttt
    12121 cgataagcaa aaattagcat gggttaataa ccaatatatg aaacaaaaag atactgaaac
    12181 agtattccaa ttagcattac ctcatttaat taaagcgaat ttgattcctg aggtgccgtc
    12241 agaagaggat ttatcttggg gacgcaaatt aattgctctt tatcaaaaag aaatgagcta
    12301 tgccggtgaa attgttcctt tatcagaaat gttctttaaa gaaatgccag ctcttggtga
    12361 agaagaacaa caagtgatca atggagagca agtaccagag ttaatgacgc acttattcag
    12421 taaattagaa gcacttgaac catttgaagc ggctgaaatt aaaaagacaa ttaaagaagt
    12481 tcaaaaagaa acaggaataa aaggcaagca attatttatg cctattcgtg ttgctgtaac
    12541 aggccaaatg catggtcctg aattaccaaa tacaattgaa gtacttggta aagaaaaagt
    12601 gctaaaccgt ttaaaacaat ataagtaatg aaatagacaa gatcaaggta gtatatactt
    12661 gaattttata acgtaaccac atatgataaa gttgtattaa caaattacaa caatatgaat
    12721 agagtatttt atataaaacg aaggattagt aattaaattt atacgatgca gagagtgtac
    12781 ggttgctgtg agtacaacgt agaaattaat gaatgcacct tcgtaaaatg aattaaatat
    12841 ataatgagag tgatgagcat taagttgaca tagtttcctt gataatttgg aagcgcccgc
    12901 aatattatta atgttattcg ctaaattcag agtggaaccg tgcggaagcg cctctaacaa
    12961 tacaatttgt atgttagtgg tgcttttttt gatatttaat ttcgcaggta cttcaattat
    13021 gtatttacgt agttcaatca ttgaggagga aatgatcttg ttaaaaagaa tgagagacga
    13081 tataaaaatg gtatttgagc aggatccagc ggcacgttca acattagaag tcattacaac
    13141 gtatgcaggt ttacatgcag tttggagtca tttgattgca cataagttat acaaccaaaa
    13201 aaaatatgtt gcagcacgcg cgatatctca aatttcaaga tttttcacag gtatagaaat
    13261 ccatccaggt gctaaaattg gaaagcgtct atttatagat catggtatgg gcgttgtaat
    13321 aggagaaaca tgtacaattg gtgataatgt gacaatctat caaggcgtga cacttggtgg
    13381 gacagggaaa gaaagaggga aaagacaccc agatatagga gacaatgttt taatagcagc
    13441 gggtgcgaaa gttttaggaa atattaaaat aaattcaaat gtaaatattg gtgcaaattc
    13501 agttgtttta caatcagttc caagttattc aacggttgtt ggtataccag gacatattgt
    13561 taagcaagat ggtgttcgag ttggaaaaac atttgatcat cgccatctac ctgatccaat
    13621 ttatgaacaa attaagcatt tagaacgaca acttgaaaag actaggaatg gagagattca
    13681 agatgattac attatataat acacttacac gtcaaaaaga agtgttcaag ccaatagaac
    13741 caggaaaagt aaaaatgtat gtatgtggtc ctacggtata taactacatt catattggta
    13801 acgcaagacc agcaattaat tatgacgtag tgagacgtta ctttgaatac caaggatata
    13861 atgtagaata tgtatcaaat tttacagacg tagatgataa attaattaaa cgttctcaag
    13921 aattaaatca atctgttccc gaaattgcag aaaaatatat cgctgctttt catgaagatg
    13981 ttggtgcttt aaatgttaga aaagcgactt caaatccaag ggtaatggac catatggatg
    14041 acattattca atttattaaa gatttggttg atcaaggtta tgcatatgaa agtggtggcg
    14101 atgtttactt tagaacacgt aaatttgaag gttatggtaa attaagtcat caatccatag
    14161 atgacttaaa agtgggtgct cgtatagatg caggagagca taaggaagat gcacttgatt
    14221 ttacattgtg gaaaaaagct aagcctggcg aaattagttg ggatagccca tttggtgaag
    14281 ggagaccagg atggcatatt gaatgttctg taatggcatt tcatgagcta ggacctacaa
    14341 ttgatataca tgcgggtggt tcagatttac aatttccaca tcatgaaaat gaaatagcac
    14401 aatcggaagc acataatcat gcgccatttg ctaattattg gatgcataat ggtttcatta
    14461 atattgataa tgaaaaaatg agtaaatcac taggtaactt tattttagtt cacgatatta
    14521 ttaaagaagt tgatccagat gtactaagat tctttatgat tagcgtacat tatagaagtc
    14581 caattaacta taatctagaa ttggttgaat cagcacgtag tggactagag cgtattcgca
    14641 atagttatca attaattgaa gagcgcgcac aaattgctac taatattgaa aatcaacaga
    14701 catatattga tcaaattgat gcgattttaa atcgttttga aacagttatg aatgatgatt
    14761 ttaatacagc taatgcaatt acagcttggt atgatttagc aaaacttgcg aataaatatg
    14821 tactagagaa tacaacatca acagaagtaa ttgataaatt taaagcagtt tatcaaattt
    14881 tcagcgatgt tttaggtgta ccgttaaaat ctaaaaatgc agatgaatta ttggatgaag
    14941 atgttgaaaa attaatcgaa gagcgtaatg aagcaaggaa aaacaaagat tttgcacgag
    15001 cagatgagat tcgagacatg ctgaaatcac aaaacattat attagaagac acacctcaag
    15061 gggttagatt taaacgtgga taatcaacaa gataatcaca ttaaattatt gaatccattg
    15121 accttagcat atatgggaga cgcagtctta gatcaatatg tacgtaccta tatcgtttta
    15181 aagcttaaaa gtaagcctaa taaactacat caaatgtcta aaaaatatgt atctgccaaa
    15241 agtcaggcgc aaacgttaga atatttaatg gagcaagaat ggtttacaga cgaagaaatg
    15301 gatattttga agcgagggcg taacgcgaaa agtcatacta aagctaaaaa cactgatgtt
    15361 caaacatatc gtaaaagttc agcgatagaa gcagtgatag gttttcttta tttagaaaaa
    15421 agagaagaac gattagaggt attattaaat aaaataataa caatagtaaa cgaaaggtag
    15481 tgacgatgtg gaagatacgg ttattgttgg taggcatgct gttagagaag cgattattac
    15541 tgggcatccg ataaataaga tattgattca agaaggtatt aaaaagcaac aaattaatga
    15601 aattttaaaa aatgcaaaag atcaaaaaat cattgttcaa actgtaccaa aatctaaatt
    15661 agatttttta gcaaatgcac cacatcaggg tgttgcagcg cttattgcac catatgaata
    15721 tgctgacttc gatcaatttt taaaacagca aaaagaaaaa gaaggtttat cgacagtact
    15781 tatattagac ggcttagaag acccgcataa cttgggatca attttaagaa cagcagatgc
    15841 aacgggagtt gatggtgtta ttattcctaa acgtcgttca gttacattaa cgcaaacagt
    15901 tgcaaaagcc tcaacaggtg caattgaaca tgtaccagtt attcgagtaa caaatttagc
    15961 taaaactatc gatgatctaa aagataatgg cttttgggta gctggcactg aagctaataa
    16021 tgcaacagat tatagaaatc ttgaagcgga catgtcattg gctattgtaa ttggcagcga
    16081 aggacagggt atgagtcgtc tagtaagtga taaatgcgat ttttatatta agattccaat
    16141 ggttggacat gtaaacagtt tgaatgcttc ggttgcagca agtttaatga tgtacgaagt
    16201 atttcgaaaa agacatgatg ttggagaaat ataatgaaag aacgttactt aatcattgat
    16261 ggatacaata tgataggaca atcacaaacg ctaagcgcca ttgcaaaaga gaatttagaa
    16321 gaagctagaa tgcaattaat agatgcaatt gcaaattata atgcagttat ttcagatgaa
    16381 atcatttgtg ttttcgatgc ttatgaccaa tcgggtgttg aaagagaata catgtatcat
    16441 ggcgttaaaa cgatttttac caaggaaaaa gaaacagctg atagtttcat agaacgttat
    16501 gtctatgaac tttatgacaa gcacactaag catattacag ttgtaacaag tgatatgagt
    16561 gagcaacatg ctatctttgg gtcaggtgca tatagaatat catctcgcga aatgtggaga
    16621 gatttaaaag aaaatgaaat tgatgtgagt aaatcattag atgatataag tgaaaacaag
    16681 ccaagaactc gaattccgtt atcttctgaa atccttgcag aatttgaaaa aatacgaaga
    16741 ggacatcata agaaatgaca tttccgtcat cttgaaattt tgaatgtaac aatattaaac
    16801 taaccttaaa ttttagatag aaggggttag tttaatactt gaaatatgat ttgacaactc
    16861 aagacagtac aatcaaacgt aacaatgcaa taaatgataa agacttcgaa aagttagtaa
    16921 tggatctaca accattaatt attcgacgca tcaaaacatt tggatttaat cattatgatt
    16981 tagaagactt atatcaagaa atacttatac ggatgtatag gtcggtccaa acatttgatt
    17041 ttagtggaga gcagcctttc acaaattatg ttcaatgttt aattacgtct gtaaagtatg
    17101 attatttgag aaaatattta gctacaaata aaagaatgga taatttgatt aatgaatata
    17161 gagttacgta tccatgtgca ataaagcgtt atgatgttga aaacaattat ttgaataaat
    17221 tagcaattaa agagttgatt cgtcagttta agtatttgag tgcatttgaa aaagatgtca
    17281 tgtatttaat gtgtgaacaa tataagccga gagaaattgc tcaactgatg catgtaaaag
    17341 agaaagtgat ttataatgcc atacaacgat gtaaaaataa aataaaacgt tatttcaaaa
    17401 tgatttgaaa agcgccttag gacgtgaatt gaattaaaac gtgttacttc ttgatggttt
    17461 gacatttgtt ataaatttta tgtatagtat actggtatta taatgaataa aggtgaatta
    17521 ttgtgaggaa aataccttta aattgtgaag cttgtggcaa tagaaattat aatgttccta
    17581 agcaagaagg ctcggcaaca agattaacct taaagaaata ttgtccaaaa tgtaacgcgc
    17641 acacaattca taaagaatcg aaataaatac attcgaaata atactttgat aatatgttca
    17701 aaggatttgg aggttgagca gatggctaaa aaagaaagtt tctttaaagg cgttaagtct
    17761 gaaatggaaa aaacaagttg gccgacgaaa gaagagctat ttaaatatac tgtaattgta
    17821 gtttctactg ttatattctt cttagtcttt ttctatgcct tagatttagg aattacagca
    17881 ttgaaaaatt tattatttgg ttagaggagt gaagacatgt ctgaagaagt tggcgcaaag
    17941 cgttggtatg cagtgcatac atattctgga tatgaaaata aagttaaaaa gaatttagaa
    18001 aaaagagtag aatctatgaa tatgactgaa caaatcttta gagtagtcat accggaagaa
    18061 gaagaaactc aagtaaaaga tggcaaagct aaaacgactg ttaaaaaaac attccctgga
    18121 tatgttttag tggaattaat catgacagat gaatcatggt atgtagtaag aaatacacca
    18181 ggcgttactg gttttgtagg ttctgcaggt gcagggtcta agccaaatcc gttgttacca
    18241 gaagaagttc gcttcatctt aaaacaaatg ggtcttaaag aaaagactat cgatgttgaa
    18301 ctcgaagttg gcgagcaagt tcgtattaaa tcaggtccat ttgcgaatca agttggtgaa
    18361 gttcaagaaa ttgaaacaga taagtttaag ctaacagtat tagtagatat gtttggccga
    18421 gaaacaccag tagaagttga attcgatcaa atagaaaagc tttaattaac aattaaagtt
    18481 attaaactaa ccaaaagata aaaaagagta ttgatttttt aattagaaaa gtgttaaaat
    18541 tatgtggtcg cgcttttaga gcgcccattt cgtcacgaaa tgttaagagt gggagggcaa
    18601 aactgagccc tgtgaccaca tcacgatatc aaggaggtgc acatcgtggc taaaaaagta
    18661 gataaagttg ttaaattaca aattcctgca ggtaaagcga atccagcacc accagttggt
    18721 ccagcattag gtcaagcagg tgtgaacatc atgggattct gtaaagagtt caatgcacgt
    18781 actcaagatc aagcaggttt aattattccg gtagaaatca gtgtttatga agatcgttca
    18841 tttacattta ttacaaaaac tccaccggct ccagtattac ttaaaaaagc agctggtatt
    18901 gaaaaaggtt caggtgaacc aaacaaaact aaagttgcta cagtaactaa agatcaagta
    18961 cgcgaaattg ctaacagcaa aatgcaagac ttaaacgctg ctgacgaaga agcagctatg
    19021 cgtattatcg aaggtactgc acgtagtatg ggtatcgttg tagaataatt ttacgaatat
    19081 taaatttgat tacatgattt aaacgatgaa gcagataaca gagataataa tgatgaatta
    19141 taaatataat ctgaatgact agattaatga ttgatttatt cataagatta attcttctgt
    19201 tgtctgctct taacttgcat ctagcaagta atgtgggagg aaattccgct aaaaccacta
    19261 aaggaggaac tataaatggc taaaaaaggt aaaaagtatc aagaagcagc tagtaaagtt
    19321 gaccgtactc agcactacag tgttgaagaa gcaattaaat tagctaaaga aacaagcatt
    19381 gctaactttg acgcttctgt tgaagttgca ttccgtttag gaattgatac acgtaaaaat
    19441 gaccaacaaa tccgtggtgc agttgtatta ccaaacggaa ctggtaaatc acaaagtgta
    19501 ttagtattcg ctaaaggtga caaaattgct gaagctgaag cagcaggtgc tgactatgta
    19561 ggtgaagcag aatacgttca aaaaatccaa caaggttggt tcgacttcga tgtagtagtt
    19621 gctacaccag acatgatggg tgaagttggt aaattaggtc gtgtattagg accaaaaggt
    19681 ttaatgccaa accctaaaac tggaactgta acaatggatg ttaaaaaagc tgttgaagaa
    19741 atcaaagctg gtaaagtaga ataccgtgct gaaaaagctg gtatcgtaca tgcatcaatt
    19801 ggtaaagttt catttactga tgaacaatta attgaaaact tcaatacttt acaagatgta
    19861 ttagctaaag ctaaaccatc atctgctaaa ggtacatact tcaaatctgt tgctgtaact
    19921 acaacaatgg gtcctggagt taaaattgat actgcaagtt tcaaataata aatgatataa
    19981 acaattacag gctgaaagaa atatctttca gtctgtaaaa atatattgac aataagtaat
    20041 ttccaagtta tattacttat tgtgattatt ttacctaaga cagtaggagt tatttataac
    20101 ttaaaattta tcctgccgag gctaaaattg acttgaacgt gatgatctat gatctttcaa
    20161 gcactttttg ccgtgggtag aaagtgcttt ttttatttaa tttaaaaaaa gcaccaaaaa
    20221 tttaaatgga ggtgtctgaa tgtctgctat cattgaagct aaaaaacaac tagttgatga
    20281 aattgctgag gtactatcaa attcagtttc aacagtaatc gttgactacc gtggattaac
    20341 tgtagctgaa gttactgact tacgttcaca attacgtgaa gctggtgttg agtataaagt
    20401 atacaaaaac actatggtac gtcgtgcagc tgaaaaagct ggtatcgaag gcttagatga
    20461 attcttaaca ggtcctactg ctattgcaac ttcaagtgaa gatgctgtag ctgcagcgaa
    20521 agtaatttct ggatttgcta aagatcatga agcattagaa attaaatcag gcgttatgga
    20581 aggcaatgtt attacagcag aagaagttaa aactgttggt tcattacctt cacacgatgg
    20641 tcttgtatct atgcttttat cagtattaca agctcctgtg cgcaacttcg cttatgcggt
    20701 taaagctatt ggagaacaaa aagaagaaaa cgctgaataa tttttagcgt aaaaaaatta
    20761 aaaataatgg aggaattata aaatggctaa tcatgaacaa atcattgaag cgattaaaga
    20821 aatgtcagta ttagaattaa acgacttagt aaaagcaatt gaagaagaat ttggtgtaac
    20881 tgcagctgct ccagtagcag tagcaggtgc agctggtggc gctgatgctg cagcagaaaa
    20941 aactgaattt gacgttgagt taacttcagc tggttcatct aaaatcaaag ttgttaaagc
    21001 tgttaaagaa gcaactggtt taggattaaa agatgctaaa gaattagtag acggagctcc
    21061 taaagtaatc aaagaagctt tacctaaaga agaagctgaa aaacttaaag aacaattaga
    21121 agaagttgga gctactgtag aattaaaata attcaagtat cttaaactta ataatcaaag
    21181 ttttatagca agtattgcta taatataatg attctttgag aagttaaaac cccgttattt
    21241 tgataacggg gttttattca tttaaagact gagtgaaatg ttataattat aatgacgagt
    21301 tacaaagtga agatgaggtg gaataatgag tcattattat gatgaagatc caagtgtaat
    21361 tagcaatgaa caacgtattc aatatcaatt aaaacatcat aaaattgatt taataactga
    21421 taacggagtg ttttcgaaag ataaagtaga ttatggttca gatgttcttg ttcaaacttt
    21481 tttaaaaacg catccacctg gtccaagtaa gcgaattgcc gatgttggtt gtggttacgg
    21541 accaattggt ttgatgattg ctaaagtatc accacatcat tcaattacaa tgctagatgt
    21601 taatcacaga gcgctagcct tagttgaaaa aaacaaaaaa ttaaacggta ttgataatgt
    21661 gatcgtaaag gaaagtgatg ctttgtctgc tgtggaagac aaaagttttg attttatttt
    21721 aaccaatcca ccaataagag cagggaaaga aaccgtgcat cgtatattcg agcaagcatt
    21781 acatagatta gactcgaacg gtgaactatt cgttgtaatt cagaagaagc aaggtatgcc
    21841 atctgcaaag aaaagaatga atgaactttt tggaaatgta gaagtagtaa ataaagataa
    21901 aggatattac attctgagaa gtataaaagc ttgaaatgaa atgaatattc tgttatagtt
    21961 atataatgta aaaatttatg ttcaataagt gtgtactttt acgttaaata gataagttaa
    22021 ttaagaataa atatagaatc gaaaatggtg tcatcattag tgttgccgtt ttctttttgt
    22081 ctttttatta atatgcttat ggtatttagc taaaagcgga tcacataatt tttgaggggt
    22141 gaatctgttt ggcaggtcaa gttgtccaat atggaagaca tcgtaaacgt agaaactacg
    22201 cgagaatttc agaagtatta gaattaccaa acttaataga aattcaaact aaatcttacg
    22261 agtggttcct aagagaaggt ttaatcgaaa tgtttagaga catttctcca attgaagatt
    22321 ttactggtaa tttgtcatta gagtttgtgg attaccgttt aggagaacca aaatatgatt
    22381 tagaagaatc taaaaaccgt gacgctactt atgctgcacc tcttcgtgta aaagtgcgtc
    22441 taatcattaa agaaacagga gaagttaaag aacaagaagt ctttatgggt gatttcccat
    22501 taatgactga tacaggtacg ttcgttatca atggtgcaga acgtgtaatc gtatctcaat
    22561 tagttcgttc accatccgtt tatttcaatg aaaaaatcga caaaaatggt cgtgaaaact
    22621 atgatgcaac aattattcca aaccgtggtg catggttaga atatgaaaca gatgctaaag
    22681 atgttgtata cgtgcgtatt gatagaacac gtaaactacc attaacagta ttgttacgtg
    22741 cattaggttt ctcaagtgac caagaaattg ttgacctttt aggtgacaat gaatatttac
    22801 gtaatacttt agagaaagac ggcactgaaa acactgaaca agcgttatta gaaatctatg
    22861 aacgtttacg tccaggtgaa ccaccaactg ttgaaaatgc taaaagtcta ttgtattcac
    22921 gtttctttga tccaaaacgc tatgacttag caagcgtggg tcgttataaa acaaacaaaa
    22981 aattacattt aaaacatcgt ttattcaatc aaaaattagc tgagccaatt gtgaatactg
    23041 aaactggtga aattgtagtt gaagaaggta cagtgcttga tcgtcgtaaa atcgacgaaa
    23101 tcatggatgt acttgaatca aacgcaaaca gcgaagtgtt tgaattgcat ggtagcgtta
    23161 tagacgagcc agtagaaatt caatcaatta aagtatatgt tcctaacgat gatgaaggtc
    23221 gtacgacaac tgtaattggt aatgctttcc ctgactcaga agttaaatgt attacaccag
    23281 cagatatcat cgcttcaatg agttacttct ttaacttatt aagtggtatt ggatatacag
    23341 atgatattga ccatttaggt aaccgtcgtt tacgttctgt aggtgaatta ctacaaaacc
    23401 aattccgtat cggtttatca agaatggaaa gagttgtacg tgaaagaatg tcaattcaag
    23461 atactgagtc tatcacacct caacaattaa ttaatattcg acctgttatt gcatctatta
    23521 aagaattctt tggtagctct caattatcac aattcatgga ccaagcaaat ccattagctg
    23581 agttaacgca taaacgtcgt ctatcagcat taggacctgg tggtttaaca cgtgaacgtg
    23641 ctcaaatgga agtacgtgac gttcactact ctcactatgg ccgtatgtgt ccaattgaaa
    23701 cacctgaggg accaaacatt ggattgatta actcattatc aagttatgca cgtgtaaatg
    23761 aattcggctt tattgaaaca ccatatcgta aagttgattt agatacacat gctatcactg
    23821 atcaaattga ctatttaaca gctgacgaag aagatagcta tgttgtagca caagcaaact
    23881 ctaaattaga tgaaaatggt cgtttcatgg atgatgaagt tgtatgtcgt ttccgtggta
    23941 acaatacagt tatggctaaa gaaaaaatgg attatatgga tgtatcgccg aagcaagttg
    24001 tttcagcagc gacagcatgt attccattct tagaaaatga tgactcaaac cgtgcattga
    24061 tgggtgcgaa catgcaacgt caagcagtgc ctttgatgaa tccagaagca ccatttgttg
    24121 gtacaggtat ggaacacgtt gcagcacgtg attctggtgc agctattaca gctaagcaca
    24181 gaggtcgtgt tgaacatgtt gaatctaatg aaattcttgt acgtcgtcta gttgaagaga
    24241 acggcgttga gcatgaaggt gaattagatc gctatccatt agctaaattt aaacgttcaa
    24301 actcaggtac atgttacaac caacgtccaa tcgttgcagt tggagatgtt gttgagtata
    24361 acgagatttt agcagatgga ccatctatgg aattaggaga aatggcatta ggtagaaacg
    24421 tagtagttgg tttcatgact tgggacggtt acaactatga ggatgccgtt atcatgagtg
    24481 aaagacttgt gaaagatgac gtgtatactt ctattcatat tgaagagtat gaatcagaag
    24541 cacgtgatac taagttagga cctgaagaaa tcacaagaga tattcctaat gtttctgaaa
    24601 gtgcacttaa gaacttagac gatcgtggta tcgtttatat tggtgcagaa gtaaaagatg
    24661 gagatatttt agttggtaaa gtaacgccta aaggtgtaac tgagttaact gccgaagaaa
    24721 gattgttaca tgcaatcttt ggtgaaaaag cacgtgaagt tagagatact tcattacgtg
    24781 tacctcacgg cgctggcggt atcgttcttg atgtaaaagt attcaatcgc gaagaaggcg
    24841 acgatacatt atcacctggt gtaaaccaat tagtacgtgt atatatcgtt caaaaacgta
    24901 aaattcatgt tggtgataag atgtgtggtc gacatggtaa caaaggtgtc atttctaaga
    24961 ttgttcctga agaagatatg ccttacttac cagatggacg tccgatcgat atcatgttaa
    25021 accctcttgg tgtaccatct cgtatgaaca tcggacaagt attagagcta cacttaggta
    25081 tggctgctaa aaatcttggt attcacgttg catcaccagt atttgacggt gcaaacgatg
    25141 acgatgtatg gtcaacaatt gaagaagctg gtatggctcg tgatggtaaa actgtacttt
    25201 atgatggacg tacaggtgaa ccattcgata accgtatttc agtaggtgta atgtacatgt
    25261 tgaaacttgc gcacatggtt gatgataaat tgcatgcgcg ttcaacagga ccatattcac
    25321 ttgttacaca acaaccactt ggcggtaaag cgcaattcgg tggacaacgt ttcggtgaga
    25381 tggaggtatg ggcacttgaa gcatatggtg ctgcatacac attacaagaa atcttaactt
    25441 acaaatccga tgatacagta ggacgtgtga aaacatacga ggctattgtt aaaggtgaaa
    25501 acatctctag accaagtgtt ccagaatcat tccgagtatt gatgaaagaa ttacaaagtt
    25561 taggtttaga tgtaaaagtt atggatgagc aagataatga aatcgaaatg acagacgttg
    25621 atgacgatga tgttgtagaa cgcaaagtag atttacaaca aaatgatgct cctgaaacac
    25681 aaaaagaagt tactgattaa tacgcaattt acaaaacagg caaaaagata ctaagctgaa
    25741 ttttattgat gattcagttt agtactttaa gccattttaa ataaatgcaa atcaatcaaa
    25801 tagcacagct aatctaaatt gaaggaggta ggctccttga ttgatgtaaa taatttccat
    25861 tatatgaaaa taggattggc ttcacctgaa aaaatccgtt cttggtcttt tggtgaagtt
    25921 aaaaaacctg aaacaatcaa ctaccgtaca ttaaaacctg aaaaagatgg tctattctgt
    25981 gaaagaattt tcggacctac aaaagactgg gaatgtagtt gtggtaaata caaacgtgtt
    26041 cgctacaaag gcatggtctg tgacagatgt ggagttgaag taactaaatc taaagtacgt
    26101 cgtgaaagaa tgggtcacat tgaacttgct gctccagttt ctcacatttg gtatttcaaa
    26161 ggtataccaa gtcgtatggg attattactt gacatgtcac caagagcatt agaagaagtt
    26221 atttactttg cttcttatgt tgttgtagat ccaggtccaa ctggtttaga aaagaaaact
    26281 ttattatctg aagctgaatt cagagattat tatgataaat acccaggtca attcgttgca
    26341 aaaatgggtg cagaaggtat taaagattta cttgaagaga ttgatcttga cgaagaactt
    26401 aaattgttac gcgatgagtt ggaatcagct actggtcaaa gacttactcg tgcaattaaa
    26461 cgtttagaag ttgttgaatc attccgtaat tcaggtaaca aaccttcatg gatgatttta
    26521 gatgtacttc caatcatccc accagaaatt cgtccaatgg ttcaattaga tggtggacga
    26581 tttgcaacaa gtgacttaaa cgacttatac cgtcgtgtaa ttaatcgaaa taatcgtttg
    26641 aaacgtttat tagatttagg tgcacctggt attatcgttc aaaacgaaaa acgtatgtta
    26701 caagaagccg ttgacgcttt aattgataat ggtcgtcgtg gtcgtccagt tactggccca
    26761 ggtaaccgtc cattaaaatc tttatctcat atgttaaaag gtaaacaagg tcgtttccgt
    26821 caaaacttac ttggtaaacg tgttgactat tcaggacgtt cagttatcgc ggtaggtcca
    26881 agcttgaaaa tgtatcaatg tggtttacca aaagaaatgg cacttgaact atttaaacca
    26941 ttcgtaatga aagaattagt tcaacgtgaa attgcaacta acattaaaaa tgcgaagagt
    27001 aaaatcgaac gcatggatga tgaagtttgg gacgtattgg aagaagtaat tagagaacat
    27061 cctgtattac ttaaccgtgc accaacactt catagacttg gtattcaagc atttgaacca
    27121 actttagttg aaggtcgtgc gattcgtcta catccacttg taacaacagc ttataacgct
    27181 gactttgatg gtgaccaaat ggcggttcac gttcctttat caaaagaggc acaagctgaa
    27241 gcaagaatgt tgatgttagc agcacaaaat atcttgaacc ctaaagatgg taaaccagta
    27301 gttacaccat cacaagatat ggtacttggt aactattacc ttactttaga aagaaaagat
    27361 gcagtaaata caggcgcaat ctttaataat acaaatgaag tattaaaagc atatgcaaat
    27421 ggctttgtac atttacacac aagaattggt gtacatgcaa gttcattcaa caatccaact
    27481 tttactgaag aacaaaacaa aaagattctt gctacgtcag taggtaaaat tatattcaat
    27541 gaaatcattc cagattcttt tgcttatatt aatgaaccta cgcaagaaaa cttagaaaga
    27601 aagacaccaa acagatactt catcgatcct acaactttag gtgaaggtgg attaaaagaa
    27661 tactttgaaa atgaagaatt aattgaacct ttcaacaaaa aattcttagg taatattatt
    27721 gcagaagtat tcaacagatt tagcatcact gatacatcaa tgatgttaga ccgtatgaaa
    27781 gacttaggat tcaaattctc atctaaagct ggtattacag taggtgttgc tgatatcgta
    27841 gtattacctg ataagcaaca aatacttgat gagcatgaaa aattagtcga cagaattaca
    27901 aaacaattca atcgtggttt aatcactgaa gaagaaagat ataatgcagt tgttgaaatt
    27961 tggacagatg caaaagatca aattcaaggt gaattgatgc aatcacttga taaaactaac
    28021 ccaatcttca tgatgagtga ttctggtgcc cgtggtaacg catctaactt tacacaatta
    28081 gctggtatgc gtggtttgat ggccgcacca tcaggtaaga ttatcgaatt accaatcaca
    28141 tcttcattcc gtgaaggttt aacagtactt gagtacttca tctcaactca cggtgcgcgt
    28201 aaaggtcttg ccgatacagc gcttaagaca gctgactcag gatatcttac tcgtcgtctt
    28261 gttgacgtgg cacaagatgt tattgttcgt gaagaagact gtggtacaga tagaggttta
    28321 ttagtttctg atattaaaga aggtacagaa atgattgaac catttattga acgtattgaa
    28381 ggtcgttatt ctaaagaaac aattcgtcat cctgaaactg atgaagttat cattcgtcct
    28441 gatgaattaa ttacacctga aattgctaag aaaattacag atgctggtat tgaacaaatg
    28501 tatattcgct cagcatttac ttgtaatgca cgacatggtg tttgtgaaaa atgttacggt
    28561 aaaaaccttg ctactggtga aaaagttgaa gttggtgaag cagttggtac aattgcagcc
    28621 caatctatcg gtgaaccagg tacacagctt acaatgcgta cattccatac aggtggggta
    28681 gcaggtagcg atatcacaca aggtcttcct cgtattcaag agattttcga agcacgtaac
    28741 cctaaaggtc aagcggtaat tacggaaatc gaaggtgtcg tagaagatat taaattagca
    28801 aaagatagac aacaagaaat tgttgttaaa ggtgctaatg aaacaagatc ataccttgct
    28861 tcaggtactt caagaattat tgtagaaatc ggtcaaccag tacaacgtgg tgaagtgtta
    28921 actgaaggtt ctattgaacc taagaattac ttatctgttg ctggattaaa cgcgactgaa
    28981 agctacttat taaaagaagt acaaaaagtt taccgtatgc aaggggtaga aatcgacgat
    29041 aaacacgttg aggttatggt tcgacaaatg ttacgtaaag ttagaattat cgaagcaggt
    29101 gatacgaagt tattaccagg ttcattagtt gatattcata actttacaga tgcaaataga
    29161 gaagcattta aacaccgtaa gcgccctgca acagctaaac cagtattact tggtattact
    29221 aaagcatcac ttgaaacaga aagtttctta tctgcagcat cattccaaga aacaacaaga
    29281 gttcttacag atgcagcaat taaaggtaag cgtgatgact tattaggtct taaagaaaac
    29341 gtaattatag gtaagttaat tccagctggt actggtatga gacgttatag cgacgtaaaa
    29401 tacgaaaaaa cagctaaacc agttgcagaa gttgaatctc aaactgaagt aacggaataa
    29461 caaatttata acagaggcta aggctttagc ctcttgttat ttttatgtaa attatttgat
    29521 ttaatgttga cgaattctct tgttcaatgt taatatatta aaggttgatg caagcagaac
    29581 tttggaggat aaattattgt ctaaggaaaa agttgcacgc tttaacaaac aacattttgt
    29641 agttggtctt aaagaaacgc ttaaagcgtt aaagaaagat caagttacat ctttgattat
    29701 tgctgaagac gttgaagtat atttaatgac tcgcgtgtta agccaaatca atcagaaaaa
    29761 tatacctgta tcttttttca aaagcaaaca tgctttgggt aaacatgtag gtattaacgt
    29821 caatgcgaca atagtagcat tgattaaatg agaattagta agtgttttac ttactaaatt
    29881 ttatttaacc taaaaatgaa ccacctggat gtgtgggatt aaaaagtgaa gagaggagga
    29941 catatcacat gccaactatt aaccaattag tacgtaaacc aagacaaagc aaaatcaaaa
    30001 aatcagattc tccagcttta aataaaggtt tcaacagtaa aaagaaaaaa tttactgact
    30061 taaactcacc acaaaaacgt ggtgtatgta ctcgtgtagg tacaatgaca cctaaaaaac
    30121 ctaactcagc gttacgtaaa tatgcacgtg tgcgtttatc aaacaacatc gaaattaacg
    30181 catacatccc tggtatcgga cataacttac aagaacacag tgttgtactt gtacgtggtg
    30241 gacgtgtaaa agacttacca ggtgtgcgtt accacattgt acgtggagca cttgatactt
    30301 caggtgttga cggacgtaga caaggtcgtt cattatacgg aactaagaaa cctaaaaact
    30361 aagaatttag tttttaatta aatcttaaaa ttaaaatatt taatataagg aagggaggat
    30421 ttacattatg cctcgtaaag gatcagtacc taaaagagac gtattaccag atccaattca
    30481 taactctaag ttagtaacta aattaattaa caaaattatg ttagatggta aacgtggaac
    30541 agcacaaaga attctttatt cagcattcga cctagttgaa caacgcagtg gtcgtgatgc
    30601 attagaagta ttcgaagaag caatcaacaa cattatgcca gtattagaag ttaaagctcg
    30661 tcgtgtaggt ggttctaact atcaagtacc tgtagaagtt cgtccagagc gtcgtactac
    30721 tttaggttta cgttggttag ttaactatgc acgtcttcgt ggtgaaaaaa cgatggaaga
    30781 tcgtttagct aacgaaattt tagatgcagc aaataataca ggtggtgccg ttaagaaacg
    30841 tgaggacact cacaaaatgg ctgaagcaaa caaagcattt gctcactacc gttggtaaga
    30901 taaaagcttt taccctgagt gtgttctata ttaatgaatt ttcattaagc gttcatgctt
    30961 agggcatcgc catatctatc gtatttattc agtaatataa actggaagga gaaaaaatac
    31021 atggctagag aattttcatt agaaaaaact cgtaatatcg gtatcatggc tcacattgat
    31081 gctggtaaaa cgactacgac tgaacgtatt ctttattaca ctggccgtat ccacaaaatt
    31141 ggtgaaacac acgaaggtgc ttcgcaaatg gactggatgg agcaagaaca agaccgtggt
    31201 attactatca catctgctgc aacaacagca gcttgggaag gtcaccgtgt aaacattatc
    31261 gatacacctg gacacgtaga tttcactgta gaagttgaac gttcattacg tgtacttgac
    31321 ggagcagtta cagtacttga tgcacaatca ggtgttgaac ctcaaactga aacagtttgg
    31381 cgtcaggcta caacttatgg tgttccacgt atcgtatttg taaacaaaat ggacaaatta
    31441 ggtgctaact tcgaatactc tgtaagtaca ttacatgatc gtttacaagc taacgctgct
    31501 ccaatccaat taccaattgg tgcggaagac gaattcgaag caatcattga cttagttgaa
    31561 atgaaatgtt tcaaatatac aaatgattta ggtactgaaa ttgaagaaat tgaaattcct
    31621 gaagaccact tagatagagc tgaagaagct cgtgctagct taatcgaagc agttgcagaa
    31681 actagcgacg aattaatgga aaaatatctt ggtgacgaag aaatttcagt ttctgaatta
    31741 aaagaagcta tccgccaagc tactactaac gtagaattct acccagtact ttgtggtaca
    31801 gctttcaaaa acaaaggtgt tcaattaatg cttgacgctg taattgatta cttaccttca
    31861 ccactagacg ttaaaccaat tattggtcac cgtgctagca accctgaaga agaagtaatc
    31921 gcgaaagcag acgattcagc tgaattcgct gcattagcgt tcaaagttat gactgaccct
    31981 tatgttggta aattaacatt cttccgtgtg tactcaggta caatgacatc tggttcgtac
    32041 gttaagaact ctactaaagg taaacgtgaa cgtgtaggtc gtttattaca aatgcacgct
    32101 aactcacgtc aagaaatcga tactgtatac tctggagata tcgctgctgc ggtaggtctt
    32161 aaagatacag gtactggtga tactttatgt ggtgagaaaa atgacattat cttggaatca
    32221 atggaattcc cagagccagt tattcactta tcagtagagc caaaatctaa agctgaccaa
    32281 gataaaatga ctcaagcttt agttaaatta caagaagaag acccaacatt ccatgcacac
    32341 actgacgaag aaactggaca agttatcatc ggtggtatgg gtgaacttca cttagacatc
    32401 ttagtagacc gtatgaagaa agaattcaac gttgaatgta acgtaggtgc tccaatggtt
    32461 tcatatcgtg aaacatttaa atcatctgca caagttcaag gtaaattctc acgtcaatct
    32521 ggtggtcgtg gtcaatacgg tgatgttcac attgaattca caccaaacga aacaggcgca
    32581 ggtttcgaat tcgaaaacgc tatcgttggt ggtgtagttc ctcgtgaata cattccatca
    32641 gtagaagctg gtcttaaaga tgctatggaa aatggtgtct tagcaggtta tccattaatt
    32701 gatgttaaag ctaaattata tgatggttca taccatgatg tcgattcatc tgaaatggcc
    32761 ttcaaaattg ctgcatcatt agcacttaaa gaagctgcta aaaaatgtga tcctgtaatc
    32821 ttagaaccaa tgatgaaagt aactattgaa atgcctgaag agtacatggg tgatatcatg
    32881 ggtgacgtaa catctcgtcg tggacgtgtt gatggtatgg agcctcgtgg taatgcacaa
    32941 gttgttaatg cttatgtacc actttcagaa atgttcggtt atgcaacatc attacgttca
    33001 aacactcaag gtcgcggtac ttacactatg tacttcgatc actatgctga agttccaaaa
    33061 tcaatcgctg aagatattat caagaaaaat aaaggtgaat aatataactt gttttgacta
    33121 gctagcctag gttaaaatac aaggtgagct taaatgtaag ctatcatctt tatagtttga
    33181 ttttttgggg tgaatgcatt ataaaagaat tgtaaaattc tttttgcatc gctataaata
    33241 atttctcatg atggtgagaa actatcatga gagataaatt taaatattat ttttaattag
    33301 aataggagag attttataat ggcaaaagaa aaatttgatc gttctaaaga acatgccaat
    33361 atcggtacta tcggtcacgt tgaccatggt aaaacaacat taacagcagc aatcgctact
    33421 gtattagcaa aaaatggtga ctcagttgca caatcatatg acatgattga caacgctcca
    33481 gaagaaaaag aacgtggtat cacaatcaat acttctcaca ttgagtacca aactgacaaa
    33541 cgtcactacg ctcacgttga ctgcccagga cacgctgact acgttaaaaa catgatcact
    33601 ggtgctgctc aaatggacgg cggtatctta gtagtatctg ctgctgacgg tccaatgcca
    33661 caaactcgtg aacacattct tttatcacgt aacgttggtg taccagcatt agtagtattc
    33721 ttaaacaaag ttgacatggt tgacgatgaa gaattattag aattagtaga aatggaagtt
    33781 cgtgacttat taagcgaata tgacttccca ggtgacgatg tacctgtaat cgctggttca
    33841 gcattaaaag ctttagaagg cgatgctcaa tacgaagaaa aaatcttaga attaatggaa
    33901 gctgtagata cttacattcc aactccagaa cgtgattctg acaaaccatt catgatgcca
    33961 gttgaggacg tattctcaat cactggtcgt ggtactgttg ctacaggccg tgttgaacgt
    34021 ggtcaaatca aagttggtga agaagttgaa atcatcggtt tacatgacac atctaaaaca
    34081 actgttacag gtgttgaaat gttccgtaaa ttattagact acgctgaagc tggtgacaac
    34141 attggtgcat tattacgtgg tgttgctcgt gaagacgtac aacgtggtca agtattagct
    34201 gctcctggtt caattacacc acatactgaa ttcaaagcag aagtatacgt attatcaaaa
    34261 gacgaaggtg gacgtcacac tccattcttc tcaaactatc gtccacaatt ctatttccgt
    34321 actactgacg taactggtgt tgttcactta ccagaaggta ctgaaatggt aatgcctggt
    34381 gataacgttg aaatgacagt agaattaatc gctccaatcg cgattgaaga cggtactcgt
    34441 ttctcaatcc gtgaaggtgg acgtactgta ggatcaggcg ttgttactga aatcattaaa
    34501 taatttctaa tttcttagat tttatataaa aagaagatcc ctcaatcgag gggtcttttt
    34561 ttaatgtgta aattttgtaa tggctattcg atttagaaga acaataattg atgaaagact
    34621 gactaataaa acttataact gataattctg tttaaataaa attgtagagt cttggacatt
    34681 gaaaaatgct cctctcaaag ttttcatttt ttcaatgtct actttgaagg gagcatttca
    34741 ttagtttatg tctcagattc atgtctttca attaatttaa atgcttaatt tgttttaaat
    34801 acttgctcta attcaatgat ttttaaaaat acagctacag cgtattttaa tgatttttca
    34861 tcaatatcaa atttgggatt atggtgtggc gctgtaatac ctttactttc attaccacaa
    34921 ccagtcagaa agaatgcacc tggtcgtact ttcaaataat gtgaaaaatc ttctccaatc
    34981 atcattaaat ctgattcatt aaaacgtaca tgtatgtcat ttgttgcttc tttaataact
    35041 tgatatgctt tctcgttgtt atggacaggc aaataccctt taatataatt caaatcgtaa
    35101 ttaatatcat ttgctattgc taaaccttgt agtagcttat ccattttgtc tattacatga
    35161 ttctgtatat ctgaatcgaa agttctaact gtacctttac aaaatgcttg atcaggaata
    35221 acgctatctg tggtgcctgc ttgaatcatt ccaaatgaaa gtacagcttg tttaactgga
    35281 tcgattgtac gtgaaattat tttttgtgca cttaaaatga actctgccat gattactatt
    35341 gggtcaatgg tttcatgagg tttggcacca tgaccgccac gacctttaat tgtgacactg
    35401 aattcatctg gagaggccat gattgccccc gcacgtgaat gaatagttcc tgtaggataa
    35461 ccactccata aatgtgtacc gtaaattcta tctacatctt ccagacatcc agcatctatc
    35521 atttcttgag aaccacctgg catgatttct tcaccgtact ggaatattaa tacaacatta
    35581 ccttctaata aatgtttatg ttcatctaaa atctctgcta cagtaagtaa aattgcggta
    35641 tgaccatcat ggccacacgc atgcatacat cctggatttt tagacttata aggcacatcg
    35701 tttaattcct cgacaggtaa cgcatcaaag tcagctctta atgcaatggt aggtcctgtg
    35761 cccaagcctt taaatgtggc tttgatacca ttgtggccga taggtgtttc aatatcacaa
    35821 gataactggc ttaattggtt aacaatataa tcatgtgttt gaaattcttc aaaagataac
    35881 tcaggatatt ggtgtaaata acgtctgagt tgaattgttt tattttcttt attatttgct
    35941 agttggaacc aatctaacac ccttatcact actttctaaa ataatgttta tagtataaca
    36001 ttttatgaaa ttatcgtact aaatgattgc tttgagatat tttatctatg aatgataagg
    36061 ctttcaagtt atgtagaatt actgtatgat aaaggtatta ccatacaata cttaaggggg
    36121 attatatact gtggttcaat cattacatga gtttttagag gaaaatataa attatctaaa
    36181 agaaaatggt ttgtataatg aaatagatac aattgaaggt gcaaacggac cagaaatcaa
    36241 aatcaatggg aaatcataca ttaacttatc ttcaaataat tatttaggac tagcaacaaa
    36301 tgaagatttg aaatcagctg caaaagcagc tattgataca catggtgtag gtgcaggcgc
    36361 tgttcgtaca atcaatggta cattagattt acacgacgaa ttagaacaaa cactagcaaa
    36421 atttaaagga acagaagctg caatagctta tcaatcagga tttaattgta atatggctgc
    36481 tatttcggct gttatgaata aaaatgatgc tattttatca gatgagctta atcatgcatc
    36541 aattattgat ggatgtcgct tatcaaaagc taaaattatt cgagttaacc attcagacat
    36601 ggatgattta cgtgcgaaag caaaagaagc agttgaatca ggtcaataca ataaagtgat
    36661 gtatatcact gatggcgttt ttagtatgga tggtgatgtg gctaaattac ctgaaattgt
    36721 agaaattgca gaagaatttg gtttattaac ttatgttgac gacgctcatg gttcaggtgt
    36781 tatgggtaaa ggcgctggta cggttaaaca ttttggttta caagataaaa tcgatttcca
    36841 aataggtacg ctttctaaag caattggtgt cgttggcggt tatgtagcag gtacaaaaga
    36901 gttaatagat tggttaaaag cacaatcacg accattctta ttctctacat cattagcacc
    36961 tggggatact aaagcaataa ctgaagcagt taaaaagtta atggcttcaa ctgaattaca
    37021 tgataaatta tgggacaatg cacaatattt aaaaaacgga ttgtcaaaat taggatatga
    37081 tacaggtgag tcagaaactc caattacacc agtaattatt ggtgatgaaa aaacaactca
    37141 agaatttagt aagcgtttaa aagacgaagg tgtctatgtg aaatctatcg ttttcccaac
    37201 agtaccaaga ggtacaggac gtgtaagaaa tatgcctaca gctgcacata caaaagacat
    37261 gttagatgaa gcaattgcgg cttatgaaaa agtaggaaaa gaaatgaagt tgatttaata
    37321 tttatttatt cccacggcaa atattgtcgt gggctttttt aaatgtttag tttattaaca
    37381 gtaagttctt ataacaatgt ttagtgttat caactattaa agtttgaatt ttaaaagcat
    37441 cttgtataat ttagttgtat tttttacaaa gaaattcatt ttgattattt ttgataatga
    37501 gcattttaat agtaatacat gtttatagtg tgtagtatat gtctatacta gtagtaacta
    37561 tatagagaaa gtaggaataa actatgtcac aagatgtaaa tgaattaagt aagcaaccaa
    37621 cgccagataa agcagaagat aacgcatttt tcccatcacc atattccctt agtcaatata
    37681 cagcacctaa aacagatttt gatggtgttg aacacaaagg tgcctataaa gatggtaaat
    37741 ggaaagtatt gatgattgct gctgaagaga gatatgtatt attggaaaat ggaaaaatgt
    37801 tctctacggg taatcatcct gttgaaatgt tattaccttt acatcattta atggaagcag
    37861 gttttgacgt tgatgttgcg acattatctg gttatccagt taaattagaa ttatgggcta
    37921 tgccaactga agacgatgca gttataagta cttataataa attgaaagaa aaattaaaac
    37981 agccaaaaaa attagcagat gtgattaaaa atgaattagg acctgattca gactatttat
    38041 ctgtctttat cccaggcgga catgctgcag ttgttggtat ttctgaaagt gaggacgttc
    38101 aacaaacatt agattgggca ttagacaatg accgctttat agttacatta tgtcatggac
    38161 cagcagcact actttcagca gggcttaaca gagaaaaatc tccattagaa ggatactctg
    38221 tttgtgtctt ccctgactca ttagatgaag gtgcaaatat tgaaataggt tatttacctg
    38281 gacgcttgaa atggttagtt gctgatttat taactaaaca aggattaaaa gtagttaacg
    38341 acgatatgac aggaagaacg ttaaaagatc gtaaattatt aacaggtgac agtcctttag
    38401 cttcaaatga gttaggaaaa ttagcagtta atgaaatgtt aaatgcaata caaaataaat
    38461 aattaaatat taattagagg agccccatat gtaaatgtat gagggctctt tttttggcaa
    38521 aatttaagtg atacttgtaa aatagaacct attatgagta tgatttaaga aaacgcttgc
    38581 aaaactaata accgcaacta gcgatatgga ggaaacatga tgtcttatag cattggaatt
    38641 gattatggaa cagcttcagg ccgtgtgttt ttaattaata caactaacgg tcaagtagta
    38701 tcaaaatttg tgaaaccata tacacatggt gtcattgaga gtgaattaaa tggtttaaaa
    38761 ataccacata catatgcact tcaaaatagt aatgattatt tagaaattat ggaagaagga
    38821 atatcatata tagtacgtga atcaaaaata gatccagtca atatagtagg tattggtata
    38881 gactttactt catctactat tatttttact gacgaaaacc ttaacccggt acataattta
    38941 aaacaattta aaaacaatcc gcatgcgtat gtgaaacttt ggaaacatca tggtgcatat
    39001 aaagaagcag agaaattata tcaaactgct attgaaaata ataataagtg gttagggcat
    39061 tatggatata atgttagtag tgaatggatg attcccaaaa taatggaggt catgaatcga
    39121 gcaccagaaa ttatggaaaa aacggcttat attatggaag cgggcgattg gattgtaaat
    39181 aaattaacta ataaaaatgt acgctcgaat tgtggattag gtttcaaagc attttgggaa
    39241 gaagaaacag ggtttcatta tgatttattt gataaaatag accccaaatt atcaaaagta
    39301 attcaagata aagtatctgc accggttgtt aatattggtg aagcggtagg gaaactggac
    39361 gataaaatgg cacagaaatt aggattatca aaagagacta tggtaagtcc ttttattatt
    39421 gatgcccatg ctagtttatt aggtattggg tctgaaaaag ataaagaaat gactatggtg
    39481 atgggaacaa gcacatgcca tcttatgtta aatgaaaagc aacatcaagt gccaggtata
    39541 tcaggttctg taaaaggagc aattattcca gaattatttg cttatgaagc gggacaatca
    39601 gcagtaggtg atttgtttga gtatgtcgct aagcaagcac caaagtcata tgtagatgaa
    39661 gcagcaaata gaaatatgac tgtatttgaa ttaatgaatg aaaagataaa acatcaaatg
    39721 ccaggtgaaa gtgggctcat tgctcttgat tggcataatg gaaatcgaag tgtattaagt
    39781 gatagtaatt taacaggttg tatctttgga ttaactttac aaactaaaca tgaggatatt
    39841 tatagagcat atttagaagc tacagcattt ggtactaaga tgattatgca acagtatcaa
    39901 gattggcata tggaagtaga aaaagtattt gcatgtggcg gtatacctaa aaagaatgct
    39961 gttatgatgg atatctatgc gaatgtactg aacaaaaaac taattgttat ggatagtgag
    40021 tatgcaccag caataggcgc agcaatatta ggtgcagtca gtggtggcgc acataattca
    40081 attaatgatg cagttgatgc tatgaaagag ccaattttat acgaaattaa tccagaaacg
    40141 gaaaaagtac aaagatatga aacattattt aaagcatata aggctttaca tgatatccat
    40201 ggatataaaa aagctaatat aatgaaagat atccagtgtt taagagttga gggataaaaa
    40261 atttaatttg atagtaatta gtaatattca acgctataaa ttactaaaat caattaatac
    40321 aactagaatt ttcatttaca gaaatatcag caacatgtac atgtcatata tacaagaaag
    40381 cgcttttata ttataattat tatgaaaatg aatattatgt ccgcttgtga tggactatgg
    40441 ttatatagaa gagacaaagg agatatttct atgaaaaaaa ttatgattac tggtgcatta
    40501 ggacaaattg gtacagaatt agttgttaag tgcagagaga tttatgggac agataatgtt
    40561 cttgctacag atattaggga acctgaagca gactcacctg tacaaaatgg accatttgaa
    40621 atcttagacg taacagatcg tgaccgcatg tttgagttag ttagggactt tgaagcggat
    40681 agtctaatgc atatggcagc attattatca gcaactgctg agaaaaatcc aattcttgct
    40741 tgggatttaa atatgggtgg attaatgaat gcattagaag ctgcaagaac ttataatttg
    40801 cactttttca caccaagctc aattggtgca tttggagact caactcctaa agttaatacg
    40861 ccacaagtaa cgattcagca acctacgaca atgtatggtg taaataaagt agcgggagaa
    40921 ttattgtgtc aatactattt caaacgtttt ggtgtagata caagaagtgt tagattccca
    40981 ggtttaatct ctcatgttaa agagccaggt ggcggtacta cagactatgc tgttgaaata
    41041 tacttcaaag cagtaagaga aggtcattat acaagcttca tagataaagg cacgtatatg
    41101 gatatgatgt atatggatga tgcaattgaa gcaattatta agcttatgga agcagacgac
    41161 gctaaattag aaactagaaa tggttataat ttgagcgcaa tgagttttga tccagagatg
    41221 gtaaaagaag caattcaaga atactatcca aattttacat tagattatga tgttgatcct
    41281 attagacaag gtatcgctaa tagttggccg gattctattg atacaagctg ttcacgtggc
    41341 gaatggggat ttgatcctaa atatgattta gcgagcatga ctaaattaat gttagaagct
    41401 attgaacaaa aagatactgt taaaaataat aactaatcat ttccattcac tttaatacac
    41461 ggaatgatat attaaattac tctttatttt aataaactag tgcatgaatt ctaataatat
    41521 tcattataca tattgaattc gcgagcttag ttcattttaa agtaaagagt tttttgtatg
    41581 taaagtatat tagtaaaaca cagattactt gtctctgtga aattaatacg tttatactaa
    41641 aagatatagt ctttctagaa aatctgttaa ttaattttga atttttagaa aatttattga
    41701 acagcaaaat atggattgtt ataatttaag ttaaacaaat tcttatacaa tattattagg
    41761 aggcaatcac catgtcacaa gcagttaaag ttgaacgacg agaaacatta aaacaaaaac
    41821 caaatacatc tcaactaggt tttggtaaat attttactga ttatatgttg agttatgatt
    41881 atgatgcaga taaaggatgg catgatttga agatagtacc ttttggtcct attgaaattt
    41941 cacctgctgc acaaggtgtt cattatggtc aatcggtatt cgaaggatta aaagcatata
    42001 aaagagatgg ggaagttgca cttttccgtc ctgaagaaaa ttttaagcgt cttaataact
    42061 cgttagcacg attagaaatg cctcaagtag acgaagcaga attgttagag gggctaaaac
    42121 aattagttga tatagagaga gattggattc ctgaagggga aggtcaatcg ttatatattc
    42181 gtccatttgt ttttgcaaca gaaggggcac ttggcgttgg tgcatcacat cagtataaat
    42241 tattaattat tttatctcct tcaggtgcat attatggtgg tgaaacttta aaaccaacta
    42301 aaatctatgt agaagatgaa tatgtgcgtg ctgttcgtgg cggtgtaggc tttgcgaaag
    42361 ttgcaggtaa ctatgcggca agtttattag cacaaactaa tgcaaataaa ttaggttatg
    42421 accaagtatt atggcttgat ggtgttgaac agaaatatat cgaagaagtt ggtagcatga
    42481 acattttctt cgttgaaaat ggcaaagtaa ttacaccaga gttgaatggc agtattttac
    42541 ctggtattac acgtaaatct atcatcgaat tagctaaaaa cttaggatat gaagtcgaag
    42601 agcgccgcgt ttcaatcgat gaattatttg aatcatatga taaaggtgag ttaacagaag
    42661 tatttggtag tggtactgca gcagttattt cacctgtggg tacattgaga tacgaagatc
    42721 gtgaaatcgt tattaataat aatgagactg gtgaaattac tcaaaaatta tacgacgtct
    42781 atactggtat tcaaaatggt actttagaag ataaaaatgg ttggagagtc gttgtaccaa
    42841 aatattaata aaaattgaat atgatcatga aaacaatatg taaaagttaa tcatgactaa
    42901 aatccctttc aatgaagcga agcatggtaa taaattaagt ttacaatgtt tattgttaag
    42961 tattgaaggg gatttcactt attattatat ttaattcaat atttaaaaag aacaatacta
    43021 cgagttcatt ttcagggaaa taacgaacat catagaatca gcataatacg aaataaataa
    43081 ataggagtat tgatattaat ggaatggata ttatttgata aagatggtac gttaattgaa
    43141 tttgatagaa gttgggaaaa aataggggta agatttgtac aatcattgct tgagactttc
    43201 ccagtacata ataaagaagc tgctttaaga caactcggtg tcattaaaga atctattgat
    43261 ccaaaatcag tgatgggttc aggatcttta caacaaatta ttcaggcatt taatgatgtg
    43321 acgggacaag atacaaccga ctggtccaag tcaacaagtc aaaagttggt agatgaacgt
    43381 attcctgaaa ttaattgggt agaaggtgtt aaagaagcac ttatcgattt gaaagcaaaa
    43441 ggctatcaac taggtattgt tacgagtgat actaaaaaag gcgtagaaca atttttagca
    43501 cataccaatg ctacctcgtt gttcgatttg atcatttcta ccgaagcgga tgcctatgag
    43561 aagccaaatc ctaaagtatt atcgccttta tttgagaaat ataatgtaga tcctcagaaa
    43621 gtagctatag taggagacac tgctaatgat atgaagacag caagtaatgc aaatttaggt
    43681 atggcaatag gtgtattaac aggtattgca acaaaagaag aattacatga agctgatatt
    43741 attttaaata gtgcggcaga tattatagaa gctttaaatt aaaaagaaag acatagtgta
    43801 tatgtgaatt aatgtattcg atagaattga taaaataatc gatgatttct atggaagtta
    43861 cattgaatta aagcacatac actatgtctt tagtctttta tcgtgtatct acttgtcgat
    43921 acgtttttat aatacgagcg attttatcaa tcataggatt taaagaatca ggatctttgt
    43981 gtatatcata ttcattaata tttatacgta caactggaca tgcattaaag ctgttaatcc
    44041 aatcgtcata acgtttaaat aactttttcc aatattcagg atcagtattt atttccattt
    44101 cgcgaccacg ttcaataata cgatcaatga cctcatcata gttacattct aaataaatca
    44161 ttacgtcagg tttaggaaaa taaggtgtca tgaccatggc attaaataag tctgaatatg
    44221 ttttgaaatc ctctttactc attgtgcctt cttcttcatg catttttgca aaaatatcaa
    44281 catcttcata aattgatcga tcttggacaa agccaccacc atattcaaac atacgctttt
    44341 gttctttaaa acgttcagct aagaagtaaa tttgcaaatg gaaactccat cgttcaaaat
    44401 cgctgtaaaa tttatctaaa tatggattat gttcgacatt ttcaaaagac gttttaaagt
    44461 ttaatttatc tgcaagtgct tgcgttagtg ttgatttccc aacaccaact gtacctgcaa
    44521 tggttataat ggcattttgt ggaataccgt aattattcat tggtaatatc tcctatcata
    44581 ggtaatataa tatgtaatat atcttcgtaa tcttgttcat ttttaagaaa atcaatagaa
    44641 gttgtatcga ttaaaactac atttgaacca ttactttgta aggactcata atatttacga
    44701 taatcttttt ttaactttaa cagatattca tcttctattt gatgctcaaa actacggtta
    44761 cgtttagcaa ttctagattt taacacatca aggtctgcat ctaaaaagat aatcatattc
    44821 ggcataatca tatcttcagt taaaatatca taaattttac tgaatttctg aaattcaaca
    44881 gaactcaaag tatttttagc aaatatctta tttttatgta tatgataatc actaactaca
    44941 ccttgattta gttgtgttac atcttgaaat tgcttatatc tattgcataa aaagaacatt
    45001 tcagtttgaa aactccattt agagatatct tcataaaagt ctgataaaaa tggattttct
    45061 gtgatgattt ctttttcttc ataaaaatct aaagtttgac ttaatttgtg tgcaagtgaa
    45121 gatttaccta cgccaatagg accttcaatt gctataaaag gtttgttcat attcacatct
    45181 ccaaatgtta aaatagacat tgagtattgt accataagtg aggtatctgg acataaatga
    45241 caaatgatat atattttatg acattagcga ttgaagaagc taaaaaagca gctcaactag
    45301 gcgaagtacc tataggtgct atcatcacta aagatgatga agttatcgct agagcacata
    45361 atttaagaga aacactacaa caaccaacgg cgcatgctga acatattgca attgaacgtg
    45421 cagccaaagt gttaggtagt tggcgtttag aaggttgcac attatatgta accttagaac
    45481 catgtgtcat gtgcgcagga acaattgtaa tgagtcgcat tccaagagtc gtctatggcg
    45541 cagatgatcc taaaggtggt tgtagtggca gtttaatgaa tttattgcaa caatctaatt
    45601 ttaatcatcg tgcaattgtt gataaaggtg tacttaaaga agcatgtagc acattattaa
    45661 caacattttt taaaaactta agagccaata agaaatccac caattagacg atagtacata
    45721 ctaattaaaa tttgttagaa ttacattata tgataaatta atgacaatat aaatgttaat
    45781 agaaaagtat gtgcgctgag tatatattct tatttaagaa caagatttta aatttacgaa
    45841 acgaggtaac ataatgataa aactaatagc cactgatatg gatggcacgc tacttaatgc
    45901 agcacatgaa atttctcaac ctaatattga tgcgattaaa tacgctcaag aacaagggat
    45961 aacggttgtt atcgcgacag gtcgagcatt ttatgaagca caagcaccag ttgctgacac
    46021 agatttaaca gtaccatata tttgtttgaa tggtgctgaa gtacgtgatg aaactttcaa
    46081 tgtaatgagc acttcacacc ttaataaatc gttagtacac aaaattacaa atgttttaaa
    46141 agatgcaggt atttattatc aagtatacac gagtcgtgcg atttatactg aagatccaca
    46201 aagagattta gacatttaca tagatattgc tgagcgtgca ggtcaacatg caaacgttga
    46261 acgtattaga aatggtattc aaagacgcat cgataatggt acgttaaaag tcgttgataa
    46321 ttatgatgcg attgaagata tacccggcga attaattatg aaaattttag catttgatgc
    46381 agatttagaa aaaatagaca aagcgagtaa aattttagct gaatctccaa acttagcagt
    46441 atcatcgtca tcaagaggta atatcgaaat tacacattca gatgctcaaa aaggcatagc
    46501 attagaaacg atagctgaaa gattaggtat tgaaatgaaa gatgttatgg ccataggtga
    46561 taatttaaat gacgtatcaa tgttagctaa agctggctac ccagttgcta tggaaaatgg
    46621 tgctgatgaa gtaaaagaaa ttgcaaaatt tatcacagat actaatgaaa atagtggtgt
    46681 aggtaaagct attatgaaat tattgcgtga acaacaagat taataaaaaa gaggggtcaa
    46741 tcatgaaagg attaataatt attggaagtg cacaagtgaa ctcacacaca aatgcactag
    46801 caacatattt aacagaacat tttaaaacac ataatattga agcggaaata tttgatttag
    46861 ctgaaaaccc attaaatcaa ttagattttt ctggaacaac tccttctgta gatgaaatta
    46921 aacaaaatat taaaaattta aaagataaag caatggcggc agatttttta attttgggta
    46981 caccaaatta tcacggttca tattcaggta ttttaaaaaa tgcactagat catctaaata
    47041 tggattattt taaaatgaaa cctgtaggct taataggaaa tagtggtggt attgttagtt
    47101 cagagccatt gtcacattta agagttattg tcagaagttt gttaggtatt gctgtaccaa
    47161 ctcaaattgc gacacatgat tctgattttg caaaaaatga agatggttca tattacttaa
    47221 atgatagtga attccaatta cgagcaagat tatttgtcga tcaaattgta tcttttgtga
    47281 ataatagtcc atatgaacat ttaaaataat attaaaaata tgtaaatata cattaaaaat
    47341 aggtatgtag cttaaggtat ttcattgaga atgcttttaa gtcacatgcc tatttttttg
    47401 ttctaatgca ccaatagtaa gcctttgagt ttaatgttgt gagcaatgtt attaattgaa
    47461 ataagatgta atagcgaaat gaaaacaagc ttagattaaa agtaaggttt aaaaatatag
    47521 ataaataatt taaagcaatt gaagaaaaaa agtattgaaa attgcaattc ttaaactgaa
    47581 atgcatatta atatttacta caaataacat tgtaaaataa aagaaaaaaa ttaaaacatt
    47641 gtactttatt gaaaagtgta ttaagataga aaggcagaaa tttttatatg caaaatatta
    47701 tatttatcaa attttgatat tttaaaggag tattattaaa tgaataataa aaagacagca
    47761 acaaatagaa aaggcatgat accaaatcga ttaaacaaat tttcgataag aaagtattct
    47821 gtaggtactg cttcaatttt agtggggaca acattgattt ttgggttaag tggtcatgaa
    47881 gctaaagcgg cagaacatac gaatggagaa ttaaatcaat caaaaaatga aacgacagtt
    47941 ccaactgaga ataaaacaac tgaaaaagtt gatagtagtc aacaaaataa tattgaacaa
    48001 agtacaacgt caaaccaacc aaaagtaaat gagtctgata atacttcagt taaagaaact
    48061 actgaagaac ctcaaaatac aacttctaca caaccaacta aacaaaataa cgatgcgacg
    48121 gcaaataaag ataatttagc ggctcaaaat atttcaacgc aggctaatga tgtttcagct
    48181 actcctaaaa caacgactat taaaccaaga acattaaacc gtatggctgt gaacacagta
    48241 gcagcaccac aacaaggtac aaatgttaat gataaagtac attttaagga tattgatatt
    48301 gcgattgata aaggacatgt taatcagact actggtaaaa ctgaattttg ggcaacttca
    48361 agtgatgttt taaaattaaa agcaaattac acaatcgatg attctgttaa agagggcgat
    48421 acatttactt ttaaatatgg tcaatatttc cgtccaggat cagtaagatt accatcgaaa
    48481 acacaaaatt tatataatgc tcaaggtaac attattgcta aaggtattta tgatagtgaa
    48541 acaaattcaa caacatatac atttacaaac tatgttgatc aatatacaaa tattagtggt
    48601 agtattgaac aagtttcatt tgccaaacgt gaaaatgcaa caactgataa aactgcttat
    48661 aaaatggaag taactttagg aaatgactct tatagtaaag aagttattgt cgattatggt
    48721 aataaaaaag cacaaccgct tatttcaagt acaaattata tcaacaatga agatttgtca
    48781 cgaaatatga ctgcatatgt aaatcaacct aaaaatacat atactaaaca aacgtttgtt
    48841 actaatttaa ctggatataa atttaatcca aatgcaaaaa acttcaaaat ttacgaagtg
    48901 acagatcaaa atcaatttgt ggatagtttc accccagata cttcaaaact taaagatgtt
    48961 actggtcaat tcgatgttat ttatagtaac gataacaaaa cagctacagt cgatttaatg
    49021 aaaggccaaa cgagcagtaa taaacaatac atcattcaac aagttgctta tccagataat
    49081 agttcaacag ataatgggaa aattgattat actttagaca ctgacaaaac taaatatagt
    49141 tggtcaaata gttattcaaa tgtgaatggc tcatcaactg caaatggcga tcaaaaaaaa
    49201 tacaatcttg gtgattatgt ttgggaagat acaaataaag atggtaaaca agatgctaac
    49261 gaaaagggta ttaaaggcgt gtatgtcatt cttaaagata gcaatggtaa agaattagat
    49321 cgtacgacaa cagatgaaaa tggtaaatat cagttcactg gtttaagcaa tggaacttat
    49381 agtgtagagt tttcaacacc agccggttat acaccgacaa ctgcaaatgc aggtacagat
    49441 gatgctgtag attctgatgg actaactaca acaggtgtaa ttaaagacgc tgacaacatg
    49501 acattagata gtggattcta caaaacacca aaatatagtt taggtgatta tgtttggtac
    49561 gacagtaata aagatggtaa acaagattcg actgaaaaag gaattaaagg tgttaaagtt
    49621 actttgcaaa atgaaaaagg cgaagtaatt ggtacaactg aaacagatga aaatggtaaa
    49681 taccgctttg ataatttaga tagtggtaaa tacaaagtta tctttgaaaa gcctgctggc
    49741 ttaacgcaaa caggtacaaa tacaactgaa gatgataaag atgctgatgg tggagaagta
    49801 gatgtaacaa ttacagatca tgatgatttt acacttgata acggctacta cgaagaagaa
    49861 ac
//
LOCUS       DAHHRR010000005        48244 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.005, whole genome shotgun sequence.
ACCESSION   DAHHRR010000005 DAHHRR010000000
VERSION     DAHHRR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 48244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48244
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.005"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            190..588
                     /locus_tag="NBI42_000231"
     CDS             190..588
                     /locus_tag="NBI42_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HCD1776697.1"
                     /translation="MKKNITKVIIASTVIATSLLTQSNDAKAFFSYEWKGLEIAKKEA
                     DQAKKDEERADKLIKEADEKNEHYKGKTVEDLYVIAKKMGKGNTIAVVKIKDGGKNGY
                     YTFDITRPLEEHRKNIPVVGSGEISEITWQ"
     gene            complement(957..1286)
                     /gene="ecb"
                     /locus_tag="NBI42_000232"
     CDS             complement(957..1286)
                     /gene="ecb"
                     /locus_tag="NBI42_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HCD1776698.1"
                     /translation="MKKNFIGKSILSMAAVSLTVSMFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVMDAQKAVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
     gene            complement(1712..2215)
                     /locus_tag="NBI42_000233"
     CDS             complement(1712..2215)
                     /locus_tag="NBI42_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCD1776699.1"
                     /translation="MSKWIIVSDNHTESGILYQIYEKHSDADVFLHLGDSEFEYNDTE
                     LSLFNRVKGNCDFYPEFENEAVIKCNDVKVFYTHGHLYQVNRTRALLAEKAHELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEIIVDDETKHGLINFKNRQYE
                     TVSHTTF"
     gene            complement(2208..2795)
                     /locus_tag="NBI42_000234"
     CDS             complement(2208..2795)
                     /locus_tag="NBI42_000234"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCD1776700.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDCRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEEKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(2807..3607)
                     /gene="racE"
                     /locus_tag="NBI42_000235"
     CDS             complement(2807..3607)
                     /gene="racE"
                     /locus_tag="NBI42_000235"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCD1776701.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(3848..4663)
                     /gene="sdhB"
                     /locus_tag="NBI42_000236"
     CDS             complement(3848..4663)
                     /gene="sdhB"
                     /locus_tag="NBI42_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HCD1776702.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(4663..6429)
                     /gene="sdhA"
                     /locus_tag="NBI42_000237"
     CDS             complement(4663..6429)
                     /gene="sdhA"
                     /locus_tag="NBI42_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCD1776703.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(6481..7095)
                     /locus_tag="NBI42_000238"
     CDS             complement(6481..7095)
                     /locus_tag="NBI42_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HCD1776704.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIVAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(7419..9200)
                     /gene="uvrC"
                     /locus_tag="NBI42_000239"
     CDS             complement(7419..9200)
                     /gene="uvrC"
                     /locus_tag="NBI42_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCD1776705.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITGFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPVRG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFIDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGSKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            9366..9461
                     /locus_tag="NBI42_000240"
     CDS             9366..9461
                     /locus_tag="NBI42_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776706.1"
                     /translation="MLDKHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(9524..9838)
                     /gene="trxA"
                     /locus_tag="NBI42_000241"
     CDS             complement(9524..9838)
                     /gene="trxA"
                     /locus_tag="NBI42_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCD1776707.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(10011..12359)
                     /locus_tag="NBI42_000242"
     CDS             complement(10011..12359)
                     /locus_tag="NBI42_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCD1776708.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEERTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEV
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(12369..14081)
                     /gene="polX"
                     /locus_tag="NBI42_000243"
     CDS             complement(12369..14081)
                     /gene="polX"
                     /locus_tag="NBI42_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HCD1776709.1"
                     /translation="MTKKDVTKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITLDDING
                     DIHMHTTFSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLNADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNI
                     KLKK"
     gene            complement(14154..14675)
                     /locus_tag="NBI42_000244"
     CDS             complement(14154..14675)
                     /locus_tag="NBI42_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCD1776710.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIVAYQNIHIFSRAMGMIVGVFITIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(14676..14942)
                     /gene="zapA"
                     /locus_tag="NBI42_000245"
     CDS             complement(14676..14942)
                     /gene="zapA"
                     /locus_tag="NBI42_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCD1776711.1"
                     /translation="MAQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            15318..16256
                     /locus_tag="NBI42_000246"
     CDS             15318..16256
                     /locus_tag="NBI42_000246"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCD1776712.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAVLHNEAIKNVLKKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGISRLDTISKKHFKNREKAQKILKPL"
     gene            complement(16558..18960)
                     /locus_tag="NBI42_000247"
     CDS             complement(16558..18960)
                     /locus_tag="NBI42_000247"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCD1776713.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSESQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELELSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DSIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     HTSNIVIEGAIFDSVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVQVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFEKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFAMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGDGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPTLAADNDLK
                     RTYVFELNFDALMAVSVGYINYEPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(18960..20018)
                     /gene="pheS"
                     /locus_tag="NBI42_000248"
     CDS             complement(18960..20018)
                     /gene="pheS"
                     /locus_tag="NBI42_000248"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCD1776714.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEEKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(20399..21139)
                     /locus_tag="NBI42_000249"
     CDS             complement(20399..21139)
                     /locus_tag="NBI42_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCD1776715.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPQYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVTYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(21379..21486)
                     /locus_tag="NBI42_000250"
     CDS             complement(21379..21486)
                     /locus_tag="NBI42_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776716.1"
                     /translation="MVLLTLVTTIHDTDDGRFFNEHKHANNKLLKRSLI"
     gene            complement(21523..21846)
                     /gene="isdG"
                     /locus_tag="NBI42_000251"
     CDS             complement(21523..21846)
                     /gene="isdG"
                     /locus_tag="NBI42_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HCD1776717.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(21865..22599)
                     /gene="srtB"
                     /locus_tag="NBI42_000252"
     CDS             complement(21865..22599)
                     /gene="srtB"
                     /locus_tag="NBI42_000252"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HCD1776718.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQAHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKTIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDKIM
                     TLSTCEDAYSETTKRIVVVTKIIKVS"
     gene            complement(22661..23626)
                     /gene="isdF"
                     /locus_tag="NBI42_000253"
     CDS             complement(22661..23626)
                     /gene="isdF"
                     /locus_tag="NBI42_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HCD1776719.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLITKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYIIAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(23639..24517)
                     /gene="isdE"
                     /locus_tag="NBI42_000254"
     CDS             complement(23639..24517)
                     /gene="isdE"
                     /locus_tag="NBI42_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HCD1776720.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMETNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(24514..25590)
                     /gene="isdD"
                     /locus_tag="NBI42_000255"
     CDS             complement(24514..25590)
                     /gene="isdD"
                     /locus_tag="NBI42_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HCD1776721.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTSSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAAK
                     KDLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDIKE
                     RRTSLFTEKGLNDISVQKDKVQQDSNKKIENERPKASGTLKVENSPPTVKKIENDHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(25590..26273)
                     /gene="isdC"
                     /locus_tag="NBI42_000256"
     CDS             complement(25590..26273)
                     /gene="isdC"
                     /locus_tag="NBI42_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HCD1776722.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKEKIISKNTAKDERTS
                     EFEVSKLNGKVDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGATTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            26482..27534
                     /gene="isdA"
                     /locus_tag="NBI42_000257"
     CDS             26482..27534
                     /gene="isdA"
                     /locus_tag="NBI42_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HCD1776723.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILSSLVYIGADSQQV
                     NAATDATNATNNQSVQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQAVLNNASFWKEYKFYNANNQELATTVVNDDKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKQNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
     gene            27737..29662
                     /gene="isdB"
                     /locus_tag="NBI42_000258"
     CDS             27737..29662
                     /gene="isdB"
                     /locus_tag="NBI42_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HCD1776724.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAEET
                     GGTNTEAQPKTEAVASPSTTTKKAPEAKSVANAVSVSNKEVEAPTPETKEAKEVKASK
                     ETKEVKPAAKADNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKENGEQQF
                     YHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVKDYAY
                     IRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAEKLLA
                     PYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEETKKALDEQVKSAITEFQNVQPT
                     NEKITDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWKDFM
                     VEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVDKEAF
                     TKANADKTNKKEQQDNSAKKETTPAKPSKPTPSPVEKESQKQDSQKDDNKQSTSVEKE
                     NDASSESGKDKTPATKPAKAEVESSSTTPTKVVSTTQNVAQPTTASSETTKDVVQTSA
                     GSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLPLMAL
                     LALSSIVAFVLPRKRKN"
     gene            complement(29815..29988)
                     /gene="rpmF"
                     /locus_tag="NBI42_000259"
     CDS             complement(29815..29988)
                     /gene="rpmF"
                     /locus_tag="NBI42_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCD1776725.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(30068..30625)
                     /locus_tag="NBI42_000260"
     CDS             complement(30068..30625)
                     /locus_tag="NBI42_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCD1776726.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLHDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            30752..31891
                     /locus_tag="NBI42_000261"
     CDS             30752..31891
                     /locus_tag="NBI42_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCD1776727.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYSKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMNVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKHAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTESLKNIYTVTEGFENRLKSNINEATDFRHFV
                     KSLKTKRYTYTHIQRLLMNVLLNIKPTDVTRNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            complement(31953..32435)
                     /gene="coaD"
                     /locus_tag="NBI42_000262"
     CDS             complement(31953..32435)
                     /gene="coaD"
                     /locus_tag="NBI42_000262"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCD1776728.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(32437..32979)
                     /gene="rsmD"
                     /locus_tag="NBI42_000263"
     CDS             complement(32437..32979)
                     /gene="rsmD"
                     /locus_tag="NBI42_000263"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCD1776729.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRYIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            33049..33438
                     /locus_tag="NBI42_000264"
     CDS             33049..33438
                     /locus_tag="NBI42_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776730.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            complement(33441..33695)
                     /locus_tag="NBI42_000265"
     CDS             complement(33441..33695)
                     /locus_tag="NBI42_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCD1776731.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            33933..34859
                     /locus_tag="NBI42_000266"
     CDS             33933..34859
                     /locus_tag="NBI42_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD1776732.1"
                     /translation="MKKVNRNILNTVLVGAGFVSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSVHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDDPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     DLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            complement(34922..35356)
                     /locus_tag="NBI42_000267"
     CDS             complement(34922..35356)
                     /locus_tag="NBI42_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCD1776733.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(35372..36409)
                     /locus_tag="NBI42_000268"
     CDS             complement(35372..36409)
                     /locus_tag="NBI42_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCD1776734.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGNIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYRLNSNFTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            complement(36736..37197)
                     /locus_tag="NBI42_000269"
     CDS             complement(36736..37197)
                     /locus_tag="NBI42_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HCD1776735.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(37222..38133)
                     /locus_tag="NBI42_000270"
     CDS             complement(37222..38133)
                     /locus_tag="NBI42_000270"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCD1776736.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLLGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            38573..39484
                     /locus_tag="NBI42_000271"
     CDS             38573..39484
                     /locus_tag="NBI42_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HCD1776737.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            complement(39625..43077)
                     /locus_tag="NBI42_000272"
     CDS             complement(39625..43077)
                     /locus_tag="NBI42_000272"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCD1776738.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSPTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKAAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGESVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(43637..44863)
                     /gene="ftsW"
                     /locus_tag="NBI42_000273"
     CDS             complement(43637..44863)
                     /gene="ftsW"
                     /locus_tag="NBI42_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCD1776739.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(45177..45452)
                     /locus_tag="NBI42_000274"
     CDS             complement(45177..45452)
                     /locus_tag="NBI42_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCD1776740.1"
                     /translation="MAKQTTMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            45593..46075
                     /locus_tag="NBI42_000275"
     CDS             45593..46075
                     /locus_tag="NBI42_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776741.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQENGYSNRINHVTHIEVI
                     "
     gene            46077..46244
                     /locus_tag="NBI42_000276"
     CDS             46077..46244
                     /locus_tag="NBI42_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HCD1776742.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(46384..48231)
                     /gene="typA"
                     /locus_tag="NBI42_000277"
     CDS             complement(46384..48231)
                     /gene="typA"
                     /locus_tag="NBI42_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCD1776743.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPDKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
BASE COUNT        15780 a         8867 c         6713 g        16884 t
ORIGIN      
        1 caataatacc tcaaataaaa ataataatat caaattcatc attattcttt ttcgattgca
       61 ttaaacctta ttaatttgca cttaatttta attaatgaag tgttaactgg taaatgtatg
      121 aaaccgtttg taaaataaca gtgtactcgc attagtaagt acaaatctat tttacaggga
      181 gaacttatta tgaaaaaaaa tattacaaaa gtaattattg catctacagt catcgctaca
      241 agtcttctaa ctcaatctaa tgatgctaaa gctttcttta gttatgaatg gaaaggttta
      301 gaaatcgcta aaaaggaagc tgatcaagct aaaaaagatg aagaacgtgc tgataaatta
      361 ataaaagaag ccgatgaaaa aaacgaacac tataaaggta aaactgttga agacttatat
      421 gtaatagcta aaaagatggg taaaggcaat acaatagctg tagtaaaaat caaagacggt
      481 ggtaaaaatg gttattatac attcgatatc actcgcccac tagaagaaca tagaaagaat
      541 attccagtag ttggttctgg tgaaatcagc gaaattactt ggcaataatt aatatactga
      601 attaaatagt tgcacgccaa acgttagaaa acaatgctaa cgttttggcg tgctttttta
      661 ttttaagtaa cttccaattt atttagcatc tttacgactg tttaataaag cacgtatgat
      721 taacacggtt gccactatat ccgttacaat ttttatgatt gttaatacat tcgatttcct
      781 attcacaata gccacctcgc ttgttcaaaa cataaacaac tattgcattc acttttaagt
      841 aattatttat atgatttatc ccaataagct caccattcaa atgaacacaa cattataaat
      901 taattatacc tttgaatata gcaaaaagcc acactctata gcatggcttc tatcaattat
      961 tttaaagtat tatattttaa aactagatcg atttgtcttt gtaatttttt cttttcatag
     1021 ctatgttgga aatgaattaa attaacagct ctttgtgctt tacggtgtgt tgcaacagtt
     1081 cttgtacgtt tgaacaagtt tacagctttt tgtgcatcca taactttttt atttacttgt
     1141 tttttaaagt ttgtttgata ctgatcatat tttttagcag cttcaacgtt tttagtttgt
     1201 gcatgagatt caccagcaaa cattgatact gttaaactaa cagcagccat acttaaaatt
     1261 gatttcccaa taaaattctt tttcataatt aataacacct atcctcaaat atttattttg
     1321 tatatattta taataataga cagatacaaa aaactacatt aactatttat taactaaaca
     1381 ttaatatttg tgtccctaca tttctgaatt tcaagaattt tgttactcca tattttaacc
     1441 accaaattat ggatataatt tatgtcttta gaaatcctta tataaatgca tacgattcat
     1501 gatattagct aaaatttcga aaatccctta cttcttagga atttatttta tactttttaa
     1561 taatacactt ttaaccaagt aatcaactct attcaaccgt atacaactat caactgtaac
     1621 tttccgaaaa atgcaggtcc tacatttaaa aattgctcgc cataaacata taaaatcaac
     1681 taaaactaaa aaaagccata gctacttttt gttaaaaagt agtatgactt actgtttcat
     1741 attgtcgatt tttaaaatta atcaagccat gcttagtttc atcatcaaca atgatttcgg
     1801 cataagtctc ttccattgaa cttcttgatt gagaaatact tcccggattt atgacatgaa
     1861 caccattaat atactcatat tttgccacat gtgtatgtcc ataaaatgca aacaaacaac
     1921 ctaactcatg tgctttttca gctaataatg ctctagtacg attgacctga tataaatgtc
     1981 catgagtgta aaatactttc acatcattac atttaatcac agcttcattt tcaaactcag
     2041 ggtaaaaatc acaattcccc tttactctat taaataggct taactcagtg tcattgtact
     2101 caaattctga atcacctaaa tgtaaaaata cgtctgcatc agaatgtttt tcataaattt
     2161 gatataaaat acctgattca gtatggttat cactcacaat aatccattta gacattttta
     2221 tcaccttcaa gaaaagcttg aagtaaatta atcgcatttc gtctatgact aatttgcccc
     2281 ttttcttctt tagaaagttg agccatggtt ttatctaatt tcggtacata aaatatagga
     2341 tcatatccga aaccattttc accatattta ccatcagcaa tttctcctga tacagtacct
     2401 ttaaatactt tagtttccat atcagggcca ctcatactta tgacacaaac aaattgcgca
     2461 cgacaatcag ttgtattacc aagcttattt aataattttt caatatttgc ttcatcgctt
     2521 ttattttcac cagcataacg tgcagagtat atacctggct caccatttaa tgcaaaaact
     2581 tctagtccac tgtcatcagc tatgaccgtt ttattcaatg cttttgcagc agcttctgat
     2641 tttaatatag cattttcttc aaatgttgat cctgtttctt ccacatcaaa atctggtatt
     2701 agttctgaaa tacctattac gtggtaatct ggaaatatca ctttaaagtc atttattttc
     2761 ccttgattat tcgatgcaat aacaatctct ttcattaaaa atcctcctag tcatttactg
     2821 atatacgttc cacattgaca gataaattta accactcttt gataatgtta gtaatgtgag
     2881 taggatcacc tgttgcaaaa aatcgatgat ctggatgttc agtataactt gcatgttcat
     2941 tactaaatgt tagcaatgca ctaacttcac gagccgtttc taatccagac gaaatcacag
     3001 tctttttacc accaaaataa tcatagatag gtttatagag caatggatag tgggtacatc
     3061 ctaaaatgac agtatcagac tcactattac gccaacgttt cagtgtttga tgaatgacaa
     3121 tgcttgtaat tgttggatca ctatatctca tttgttctac aagtggcaca aaacctggac
     3181 aggcaacgcc atgtacctct acatgtggat ttatacgttt aatatgcgta cgatatgctt
     3241 cagatttaat tgtgccttcc gttcctagta ctaatacatt ttgatttcta gtagtcatta
     3301 ttgctgttct agcacctggt tcaattacgc caatcactgg gattgataag gtcttttgta
     3361 aatattctaa agctacagca gttgcagtat tacatgcaat cacgagcatt tttatatcaa
     3421 attccattaa tttacgagcg atttcaactg tatattgttt tacttgttct cctggtcttg
     3481 gcccatatgg acatcgccca atatcaccta agtaataaat cgtctcattt ggcaactgac
     3541 gcataatttc tttagctact gtcaaacctc cgacaccaga gtctattaca cctattggtt
     3601 tattcatatt cggtcatcct tacttttaat ttaattttta gtttatcata actaagcatt
     3661 ggattttagt attatgcact gtgtttacca tttttgtcat tataatataa tacttgtttt
     3721 aaatcagccc actatcatat tgtcatgtaa tctgcttatt aaaaaaatcc cttctaagtt
     3781 attgtgtatc tccattcaat ttaattttga aaggaacata acattttaac tcaaaaggga
     3841 ttaattttta ttctacttca tggtctgaac caaagaatga tttaaacatg tggaatgttg
     3901 tttctctgtt cattgctgca atggatgttg ttaagggaat acctttaggg caagcattaa
     3961 cacagttttg tgaattacca cattgttgta aaccgcccgt acccatcaat gcgtttaaac
     4021 gttcatcttt agtcattgat ccagttggat gtaagttaaa taaacgtact tgtgaaattg
     4081 cttgtgcacc aacgaattta ttattttcag taacgttagg acaaacttct aaacatacac
     4141 cacaagtcat acatttcgat aattcatatg ctgtttgacg tttcttctca ggcatacgtg
     4201 gtccaggacc taaatcataa gtaccatcaa ttggaatcca agctttcata cgtttcaagt
     4261 tatcaaacat tcttgaacga tcaacttgta agtcacggat aactgggaaa gtattcattg
     4321 gctctaaacg aataggttgt tctaattgat caacaatcgc agaacaagat tgtcttgcac
     4381 gaccattgat aaccatagaa catgctccac atacttcttc taagcagttc atatcccaga
     4441 caacaggtgt tgttttttca cctttaatat taactgggtt acgtctaatt tccattaaac
     4501 atgcaatgac gtttaaattt tcacgatatg gaatttcaaa tgtttcttca taaggcttag
     4561 aatcacttgt atcttgtcgt ttaatgatta attttactgt tttttgtttc ggtttagatt
     4621 gtgtttcatg ttgtggagtg tttttcactg attgttcagt cattattttt tacccccttt
     4681 agacttactt gtataatcac gtttacgtgg tggtattaaa ctcacatcga cgtcatcata
     4741 agtaaattgt ggcttttcaa atgcaccttg gaatgaggcc attgttgttt ttaaccactc
     4801 ttcgtcatta cgttctggga attcaggttt ataatgtgca cctctagatt cgttacgatt
     4861 atatgcacca atcgtgataa cacgagcaag cactaacatg ttccataatt ggcgagtaaa
     4921 gaataccgct tggttactcc aagtttgagt atcttccata tcaatatctt cataacgttt
     4981 catcaattca acaatctttt tatctgtttc taacagtttt tcattttcac gaacaacagt
     5041 tacatttgct gtcataattt caccaagttc acggtgtaat ttatatgcat tttctgtacc
     5101 gcgcatagct aataatttat caaaacgttc ttgctcttca gctttacgtt tttcaaaaat
     5161 actttcgtcc atatcagtat atgatcgatc aatatttgaa atataatcaa tcgcgtttgg
     5221 acctgctact gtaccaccat aaatcgctga taacaatgaa ttggcaccta agcggttacc
     5281 accatgttga gagaagtcac attctccagc tgcaaataac cctttaatat ttgtcatttg
     5341 atcataatct acatatagac cacccattga atagtggaca gctgggaaaa tcttcattgg
     5401 tactttgcgt gggtcatcac cagtgaattt ttcataaatt tcaatgatac cacctagttt
     5461 tacatctaac tcatgtggat ctttatgtga caaatcaaga tataccatgt tttcgccgtt
     5521 aatacctaat ttttggttaa tacatacatc gaaaatttca cgcgttgcaa tatcacgagg
     5581 tactaagtta ccataatcag gatatttctc ttctaagaag taccaaggct taccatcttt
     5641 atatgtccaa attcgtccac cttcaccacg tgctgattca ctcattagtc gtagtttatc
     5701 atcaccaggg attgcagtag gatgaatttg aatgaactca ccattagcat aaatagcgcc
     5761 ttgttggtaa acaatggaag ccgctgatcc tgtattaatc attgagtttg ttgttttacc
     5821 gaagatgata ccagggccac ccgttgccat aataactgca tctgaaccaa atgtttcaat
     5881 ctcagcagtt gtcatatttt gtgcaacgat acctcttgca ctatcatcgt cacctttaac
     5941 tatgccaagg aattcccatc cttcatactt cgtaactaat ccatctactt catatgcacg
     6001 aacttgctca tccaatgcat ataataattg ttgtccagtt gttgcccctg catatgctgt
     6061 tctgtggtgt aatgtaccac cgaaacgtct aaaatctaat agaccttcat ttgttctatt
     6121 gaacattacg cccatacggt ctaataaatg aataatttta ggtgctgcct ctgtcatcgc
     6181 tttaacaggt ggttggtttg caaggaagtc gccaccatac actgtatcat caaagtgaat
     6241 ccaaggagaa tcgccttccc ctttagtatt gaccgcacca ttaatgccac cttgggcaca
     6301 aacagagtgc gaacgcttta ctggtacaac tgagaacaaa tctacatgtg cacctttttc
     6361 tgccgcttta attgttgaca ttaagcccgc taggccacct ccgacaacaa taagatgttt
     6421 ctctgccata aaaatttcac tcccctaaat tttcaatcta tatttgttaa atgcgatgta
     6481 ttacataaag gcaataattg cagtaacacc aatatacgaa ataactaaga atacgattaa
     6541 tgaaacccat gtaaatactc gttgtgattt tggagattga agtccacccc aagtaactaa
     6601 gaatgaccat aagccatttg caaagtggaa cacaacagca acaatacaaa taatataaaa
     6661 tattgcccat ccaggatgtt gcaatgtttc gtgcattaaa tcgtaattca cttctttgcc
     6721 gtaaaatgct ttttgtaaac gtgtttgcca taaatggata ccaataaaga taaatgttaa
     6781 gataccactc actctttgga agaagaacat ccagtttcta aatatcgagt aatgtccaac
     6841 attttctttt gctgtaaatg caatgtgtat accaaacaaa ccgtgatata acaacggaat
     6901 gtatataaat aaaaattcta caataattag aaatggtaat gattccataa agttagatgc
     6961 cttattaaac gcttcagcac cttgtgttgc ttggtggttc actaataaat gaacgaccaa
     7021 aaatgcacct attgggataa tacctaataa cgagtgaata cgtcttagat aaaattcatt
     7081 ttttgattga gccaaaagga gtcccccctg tgaacgaata tttaatttat tgagctattt
     7141 atattaaacg tacgcttaac cccctaaagt gatatcgttt ctagcgttgt cattatcatt
     7201 aagcgaaaca ttttaaaaac aaatacacac tgcacgatca ccaaactgca tgtcgaacaa
     7261 tgtaacattt ggatttaata tttaagattg cttgtgatga taaactttct catttagaaa
     7321 acgcttccac gtacattcaa aaaaataact ttgttaacca tattgtaaca ttatttcata
     7381 tattttgggg catgagaatg attctcacgc cctgtaattt atttatgcaa ttgttcatgt
     7441 aggttctttg caacgttttc aggaatacct atatttttaa aatcttcaag tgtagcttcc
     7501 ttcattttct tgattgaacc gaatgaacgc aataataatg ttttacgttt gctaccaata
     7561 ccatctatat catcaaggat tgatttcaag cctgtctttt gacgtgtttg tctatgaaaa
     7621 gtgattgcga atctgtgaac ctcatcttgg atacggtgca acaaataaaa tgcttggcta
     7681 tttttcttca gtggtacaat ttctgcacta gcgccatata ataattcaga tgtttggtgt
     7741 ttatcatttt tttgcaaacc tgcaacaggg atatcaagac ctaattcgtt ttgtagcaca
     7801 tcaataaccc cgttcatatg tcctttacca ccatctacta ttattaaatc aggtaatggt
     7861 aatccttcgt ttaaaacgcg agagtatcgg cgtcttacta cttctctcat tgatttgtaa
     7921 tcatctggac ctttaaccgt tttgatttta tactttctat aatttttttt gtctggttta
     7981 ccgtcgataa atgtaaccat tgctgacact ggatccacac cttgaatatt agaattatcg
     8041 aatgcttcaa ttctaattgg tgtttgaatt cccatttgtg ttccaagttc ttcaatagct
     8101 ttaattgttc tggactcatc acgtgatatt aattcaaatt tattatttaa ggatacttta
     8161 gcgttatgtg cagctaggtc aaccatatct tttttgggac ctcgcacggg ttgaacgatt
     8221 ttagtgtcca caacagattg aatcatttct ttatccaaat tacgtggtac atgaacttcc
     8281 ttaggtaaaa tatgttggtt taagctataa aattgtccaa taaatgtata aaattcttct
     8341 tcttctgttt gctgtaatgg aatcatcgtt gtatctcgct ttatcatatt accttgtcgt
     8401 ataaagaaaa cttggataca catccatcct ttatcaacac tataaccaaa gacatcacga
     8461 atcgttttat ctgatgacat aattttttgt ttattagtta gattttgaat atgttgaatt
     8521 aaatctctat attctttagc ccgttcaaaa tcaagtgatt cacttgcagt taacattcgc
     8581 tcttctaaac tttttaaaat tgttttgtct tccccattca gaaaaccagt aatttccttc
     8641 gtcatttgtg cgtatttact caaatcaacg tcatatacac atggtcctaa acattgtcca
     8701 atatggtaat aaagacataa tttatctggc atcttatcac atttgcgata tgggtatatt
     8761 ctgtctaata attttttagt ttcttgagca gaatatgcat tcggatacgg tccgaaatat
     8821 ttgccagtac cttgttttac agttctcgtc actagtagtc taggatattt ttccttcgta
     8881 attttaataa atggataact tttatcatcc tttaataata tattatatct tggttgatat
     8941 tgtttaatca gattcaattc aagtaaaagt gactctgttt cactagacgt gacaataaac
     9001 tcaaagcgac gtatttcacc aaccagtctc gttgttttag catcatgagc gcccgtaaaa
     9061 tatgatcgca atcgatttct tagcttttta gctttgccaa catatatcac ttgatcatta
     9121 cgatctttca ttaaatagca gcctggttcc ataggtacga catttaattt atttttaatt
     9181 cgttgcttat agtcttccaa aaatatcccc ccttctaatt ttaaaatgaa tgatgctaca
     9241 aaagtgcttc aaatttatca ccacttattc tagttcataa tcaacatctt cgaatatgac
     9301 ttttcataca catcatgtaa tattgaacat tgttttatca aaccaactgc tacaaatcta
     9361 tcagcatgct agataaacat ttaacgttca tagatttatt aatcccacca ttctcaagtc
     9421 gtaaacaata tgatgactta tcattcaact ttttatatta aaaacacaaa caaaaactgg
     9481 agcaattcat taaagaaatg ccccagtcgt cattggttgt aacttataaa tgtttatcta
     9541 aaacttcagc taagttttct tttggttgga aaccaacaac tttatcaact ggttgaccgt
     9601 ctttaaagac gattaatgtt ggaatactca tcacttcata tttagctgca gttgatggat
     9661 tttcatcaac atctaatttt aaaatgtcag ctttaccttc atagtcagct gctaattctt
     9721 ctaataccgg agcgatcatt ttacatggac cacaccatgt tgcccaaaaa tctactaatt
     9781 gtacaccaga ttctactttt gaatcaaaat ctgcatctgt tacttttacg attgccataa
     9841 atgccaatcc tccttaatta ttattatgat gttcaaatta tatcagataa catgcttcat
     9901 gtatttctat ttgctcaatt tttatgctta tttcaaataa catgtttgaa atgtattctt
     9961 gttgctttac attattttaa ctgcagctat ttaatttatc aaattataat ttattttagt
    10021 gttgcaacgg taacgccaaa tccaccttcg cttggcatac cacctctaaa gtcactaaca
    10081 cttttatgct ttttcaaatg ttgttgtaca cctttttgaa gtgcacctgt acctttacca
    10141 tgaatgatat aaacttgttc gtaattactt aaaacggctt gatctaaata ttgatctagt
    10201 tcaattaaag catcctcata acggtagcct cgtaagtcaa gttcagtttt aattgtttga
    10261 cgattttgac gtgtaaccat ttttgttggt ttaacttttt ctttttgttt tttctctaaa
    10321 tcttcaatag gtaacttcat tttaataatt cccatttgaa caattgcttc ttcatcattg
    10381 acaatttcta aaacttcacc cttttgaccg taagataata cttttacttc atcaccagca
    10441 acaattttat cgtatttttg cttttgtaca ttttgcttta tagatttcac ttcataatga
    10501 tcatctaatc gtttcttctt atcaatcaat tcatgttctt taacatctgc acctttttgt
    10561 tcacgcaatt gtcttaaatc tttaataata tcgtcagctt cttttgttgc agctttaatc
    10621 ttctgatttg ctttttcttt cgcttcctct attagagatt tttcataatt ttggaattgt
    10681 tgatactgct tagataaatc atcatgcact tgctccgctt cttttacaag acggtccagt
    10741 tctaaccttt gtgtctctac acgtttgtaa ttacgctcta atgattcaat catttcattt
    10801 atttcttttt catcagtacc aatcatcgtc ttagccttat taataatatt caaactaaga
    10861 cctaactttt tagaaatatc aaaagcattt gaacgacccg gcacacccat taataactta
    10921 tacgttggac tcaaagtatc tacatcaaat tctacactcg cattcataac gccttctcga
    10981 ttataactat atgctttaag ttcaggatag tgcgtcgttg ccattactag agaaccaatt
    11041 tttctaacat ggtctaaaat gctcattgct aaggcagcac cttcacttgg atctgtacct
    11101 gcacctaatt catcaaataa aactaaacta tgtttgtctg catgctttaa aatctcaact
    11161 atattcgtca tatgagatga aaaagttgat aatgattgtt ctattgattg ttcatctccg
    11221 atatcgcaat atacattttt aaatacactt aactgactac catcaagtgt tggaatcaac
    11281 aatcctgatt gagccataac aataattaaa cctaatgttt ttaatgttac tgttttacca
    11341 cctgtattcg gtcctgtaat aattaccgtt tcaatatctt ccataaattc gatggtatta
    11401 gctacaactg tctcacgatt taataatgga tggtatgctt taggtaaata tacagtacgt
    11461 tcctctttaa atatcggctt tgttccttta atacttctac tatatctcgc ttttgcgatt
    11521 aaaaaatcta actgacccat gacttgttct gccacaagca gtgcatcttt gtccgcagcc
    11581 acataaccag ttagttgcgt taaaatgcgt tctttttcaa ttgcttcatc atgacgtaat
    11641 cgactaattt gattattcat ttcaacaact gatgatggct caatatacaa tgtttgtcct
    11701 gaagcagatt gatcatgtac aatcccatta aaatcttgtc gatattcagc tttgacaggt
    11761 ataacatttc tttcattcct aactgttaca atagcatctg ataatttttt ctgatttgct
    11821 tgacttttaa caatacggtc caaattttgt ctaatacgtt gattcgtgct agaaatttta
    11881 cttctaatcc cttgcaattc ataactcgca ttatcatata aatcgtacgt atcacatgtt
    11941 tcatttattt gttgaaaaag atctgttaac acaggtaatt gattcatctt gtcatctaat
    12001 attgggtatt taacaccttc atcttcttca accaattgat tataaaatgt cttaaattga
    12061 ttttgtactt gaattaatct ttttatcaag ttaagctctg atacatttaa aacgccgcca
    12121 atatcagcgc gatgaatgaa tgctgatact ttagataagc cactcaagct tggtaaacga
    12181 tgcttattat agatttgagc aatctcatcc gtttcttcca tttgaaaaac aaccgtttca
    12241 aaattagtag ctggcatcat ttgattgacc ttttccaagc ctaagtcact aatagtttca
    12301 ttggcaacga gtgattttat tttttcaaat tctaagacgt ctaatgtttt ttgtctcata
    12361 aaatccctct atttcttaag ttttatatta ttttcaataa agtctttaaa agcttcacgc
    12421 gataatgcgt taatcactct atcttttgtt acaaatcctt tttgtgcagt tgctacgcca
    12481 taattcataa aatctaaatg atttgtatga tgcgcatcag tgttaatagt taatttcaca
    12541 tttggatatt tacgaacgat atcagcattc agatccagtc gatgtggatt ggcattaatt
    12601 tctaatactg tattcgtttc ttcagctaat gccattaatt gttcaatatt cggtttataa
    12661 ccatctcttc tacctataat tcgccctgtt ggatgcgcta tatgtcgcac gtatggattg
    12721 cgacatgcat tagctaaacg ttccataatt tgctcttctg attggttaaa gctttgatga
    12781 atagctccaa ttacataatc aagttgtgct aaaatttcat catcataatc cagcgagcca
    12841 tcaggtaata tatccatttc tgtacctgaa taaatatcaa tttcactata ttctttatct
    12901 aaagctttaa tttcttcgtt ttgtcttaaa agtctttcta cttgtaagcc attagcaaca
    12961 cgtaaacttt gtgaatgatc agtaattacc atgaatttat aaccttttgc gatatttgct
    13021 tctaccatgt ctcgaataga aaacgcacca tcactaaacg ttgtatgcat atgaatatca
    13081 ccattaatat catctaatgt aatgatatta cttagatctt tatcaaattc gctaccatct
    13141 tctcgcatag caggtggtat aaaattcaca ttaaaatgtt catatatttt ggcttcacta
    13201 tcatattgaa ttaatgtacc atcagcttgt tcaattccat attcacttac tttttcatca
    13261 cgtgctttag caagttgtcg aattcttata ttatgttctt ttgacccagt aaaatgctgc
    13321 aatgtatgat aaaaagcact tggttcaatt aatcgaaaat cgacaccaat cgtttcatca
    13381 tcatacgcta attctaatga aacttttgtg ttccccactg caacttcttt tactttattg
    13441 ggaatattta ataattgctg ctgcactgct tttgggttat cggtacttat tatgaaatct
    13501 aaatctttgc tcatttcttt aaaacgacgg aagcttcctg cagatgaata ttgatcgata
    13561 taatttaatg tatctatata atcaatgatt tcttgattaa gtcttctcat ttgatcaatt
    13621 ggatatctat ctttcttagc accaagttgt ttcacagctt ctaatatgtt ttgttccgtt
    13681 ttcttagcaa atccgcttaa ttcactaact tttccatttt cacaagcaac ttgaagtgac
    13741 gctttatcaa caatattcaa ctctttatat agcttagcaa ttttcttgct tccaagtcct
    13801 tgaattttca aaagtggaat aagaccttcc ggaacttctt cctgtaattg ctgtaaatac
    13861 tgagattcac cggtctcacg gtaatcattg attacttctg caacaccttt accaatgcct
    13921 tttaactccg ttacatcaga tatttcatct aatggtcgtt catctaattc aagactttga
    13981 gctgcttttc gatacgcaga tattttaaaa gtattttcac cttttaattc catataagta
    14041 gcaatttgtt ctaatagttt ggtaacatct ttttttgtca taataacact ccataaaaag
    14101 aagaccagga cgtatcatta atatatacct ttgtcctgac ctcttatgat aatttataaa
    14161 ttaatggtga aagccgataa atatgggatg tgaaaaatca atgaatgact cacaatagat
    14221 attttaagct gatgttgtaa tgcttcgtta ggatataatg ccaatagata taacgtaaag
    14281 tgtaagacaa ttatcgttat aaacacacca actatcattc ccattgcacg actgaaaata
    14341 tgaatgtttt gatacgctac tattttatca aaagttacga taattagata taaaatgaac
    14401 ttacaaaaca atgtaatcat taaaaaagct acaatcgctt caaatcgatt ttgtagatga
    14461 ttaaaatgaa acgcaaaagt tgtattaaat gctgttgttt taggatatgg aataaataca
    14521 attaatcttt ctacaataga tttgtaaaat tgactggcaa tccacaatga tacaatcgtt
    14581 gcactcaaat gtatcataga taaccaaaaa cctcgtctga atccaacgat gacaaaatac
    14641 acaaagaaaa tgattatgat aaaatcaatg accatttatt gctcacgctg ctgcaatttg
    14701 tgaatttgtt gtttcaaacg acgattttct tcttctagta gtactttttc atgcataata
    14761 ttcacagcag ttagtattgc ttttcttgaa gtatctaaac ctgctgcttt ataccctaat
    14821 tcttttattt tatcatcaac taaatgtgct acatatcgta tgtgctctgg gttatcttcc
    14881 ccaacaattg taaaaagctg atcattaatt gatacattta ccttgttttt aaactgtgcc
    14941 atttataatt tctcctgatc ctttttttaa aaatctaaat tcacgttata aaatatgact
    15001 ggatagtttg tctgaatttg atactaatat tgttatattg caattatgat aaaacaacaa
    15061 cacaatctct atagatgact taatgttctt tttataatga aatgaaataa tgtaaagaat
    15121 tttctattca atactttatc atgtttaaat tgtgtcacta taacattttc ataaacatta
    15181 tacatgacca ctatgtattt tgtaagtatc cgcaattaat tctttacaac atatataaat
    15241 gtttctgacg ttactatcat ttatgatatg attatttttg ctaatgataa tgaaattata
    15301 tgaaaggatt tacacaaatg gcgaatatcg tttttaaatt gtcggataaa gacataacga
    15361 cattaatgtc acgcatttct tttgatactg agaatttacc tcaaggtatg aaagcacgtg
    15421 caaagtatca aaatacaact gtaaatattt accaatcagg taaagtcatg tttcaaggta
    15481 atcatgcaga agctgtgtct gaagaacttc taccgcagca ttctcaattg aatactaata
    15541 aaactaaaaa gaaaaacatg gctaattctt ttcttgaaca aactttaatg tatgatcaat
    15601 ttaactgtat tggtagtgat gaagctggaa gtggcgatta ctttggtcca ttgactgtat
    15661 gtgctgcttt cgttaccaaa gagcatgtcc ctattttaaa aactttgggt gtagatgatt
    15721 caaaaaaatt aaccgatacc aaaattgtag aacttgctga acaattggtt actttcatcc
    15781 cacattcact tttaacacta cacaatgaaa aatataatat tcagcaagca aaaggctgga
    15841 ctcaagtaaa aatgaaagct gttcttcata atgaagccat caaaaatgta cttaaaaaaa
    15901 ttgattcgtc tcaattagac tatattgtca ttgatcagtt cgcaaaacgc gaagtgtata
    15961 gccattacgc attatccgat ataccacttc caaagaaaac aaaatttgaa acaaaaggag
    16021 aatcgaagtc attagccatt gcagtggcaa gcataatttc tagatacgca tttgttacgt
    16081 atatggatca aatatcaaaa aatattaata tgacaattcc taaaggtgcc ggtgctaaag
    16141 tcgatgtaat cgccgccaaa attataaaaa aatacggtat ttcacgttta gataccattt
    16201 caaaaaagca ttttaaaaat cgtgaaaaag cccaaaaaat tttaaagcct ctttgatttt
    16261 taatcattag aggcttttat gcagttgtgc aaagcatcaa ctgtattccg ctgttcctct
    16321 ttgattttta atcaagataa attggatcct aataggaaat cctattaggt aagtcatact
    16381 aatttgcttt gctcataagt atgactttat atgcagttgt gcaaagcatc aactgtattc
    16441 cgctgttcct ctttgattta taaatgaatt ggaacctaat tggtgaatcc aaaaataaaa
    16501 ccccatgtta aggatatcaa tctacttcag atatccttat acatggggtt taaatcatta
    16561 tctaataaca gcaccttgtt caattaatgc tgcttcaatt tccgcttgta cttttgaaac
    16621 gcgttcatct gtcaatgttt cttctgtgtc taaataattt aatcgtattg caattgattt
    16681 tttacctttt tctaaatgtt caccctgata tacatcaaag acaagtgtgt cttttaatat
    16741 attgccaccg tgtgcatgaa tcgttgataa taaatcagct gctggaatat tttgatctac
    16801 ttctaatgca atgtcacgag acatgcctgg gaatctcgga attggctcgt aattaatgta
    16861 acctaccgac acagccatta atgcatcaaa attcaactca aaaacatacg tacgttttaa
    16921 atcattatct gctgctaatg ttggatgtaa ttcaccaata aaaccaacaa ctttattctc
    16981 taataagatt tcagctgtac gacctggatg taatccatca atatcagcac gacgataact
    17041 aaattcaagg tttaactttt cagatactcg atccacgaca ccttttgcta aatagaaatc
    17101 aaccgtttct ttcttacctt gccattgatt gactacataa tctccagtta aaataccact
    17161 taaatattca acttgatctg gtagttcacc atcaccatta gcaaagaaga cattgccgat
    17221 ttcaaataat tttacatctt tatttttgcg tgccacatta tatgatgccg cttcgattaa
    17281 atgtggtaat aaactttgac gtaatgacgc atgcgcttca ctcattggca tcaataaatc
    17341 aattgtttga cgctgttgca tcgcaaatgc agtagcatct tctttagaaa ctaacgaata
    17401 cgtaatagct tggtctaatc cagcaccttc taacacttct ttaaccattc tagttttata
    17461 ttggcgatca gttagctgac cactagtaac tttttcgaag acaggtaacg ttgatggaat
    17521 atcgtcgtaa ccatatatac gtgcaacttc ttcaattaaa tcttctttaa ttgtaatatc
    17581 tttacgacgt gatggtactt gcactgtaat aacatcatca tttatttctg tatcaaaccc
    17641 tagttgatta aaaatagtaa caatatcatt ttgtgacaaa tcaaatccaa tagtgcgatt
    17701 aattttatca gcagtgatgt cgattggtgt tataaatgca ccaagttctc ctgaagatac
    17761 tctatctttt agcacttttc cgtttgcata agtttgtaat aaataacatg cacggtcgac
    17821 tgcttcatct acaaattcag tagctattcc tttttcaaaa cgactagatg attcactgcg
    17881 taaatttaaa cgtcttgatg tatgacgaat ggaaacagaa tcaaaaatag caccttcaat
    17941 aactatattt gatgtatgtt ctttaacttc tgaaaaatcg ccacccataa caccagctaa
    18001 tgcaattgga gtttgtccat tagtaatgac aatatcgctc gttaataatt cacgttctgt
    18061 atcatctaat gttgtcattt tttcgccttc attagcttga cgaacaacaa tttgttgtga
    18121 accaatcgaa tcttgatcaa acatgtgcaa tggttgaccg tattctaata acacataatt
    18181 tgaaatgtcg acaacattat taatagggcg tatacctgct tttattaagc gtgcttgcat
    18241 ccaaattggc gagggttcaa ttgtcacgtc gtgaacaaca cgtgcactat aatatggtac
    18301 tttatcttca ttttcaattg tcacagtcag ttcatcattt gcagataact caagctcatt
    18361 tgatgttgtc tctggcttag tcatttttgt attatataat gccgcaactt cataagcagt
    18421 acctatcata cttaaagcat cagcacgatt cggcgttaaa tcaaattcca ttacttgatc
    18481 atctaaatat aaagcttgta aggcatctgt tcctggaact tgggattcac taaatacata
    18541 aatgcctgat tcaaaacttt tcggtatata gttacttgaa ataccaattt cttgtaacga
    18601 acaaatcata ccttctgaac gttcaccgcg taatttggca cgcttaattt taataccacc
    18661 aggcaatctt ccacctactt tagcaacaat gacatattgt cctgcatcaa cgttcggtgc
    18721 accacatact atttgtacag gttcgtcttc accgatatca acttggcaaa catttaattt
    18781 atcagcatca ggatgtttct cttttgactt aacgaagccg acaactaaat ttttgatatc
    18841 ttttgtgtag tcaattaaat catccacttc aatacctgtg cgcgtaatac gttctgccaa
    18901 attacttaca gaatcatcga ttgttacata ttctttcaac cattcatttg atatcaacat
    18961 tacatgtcac ctctatcttc taccgcttta aattgatcta aaaatctcac atcattagta
    19021 tagaaatgac gaatatcttc tataccatat ttcaacattg caatacggtc tggtcccata
    19081 ccaaatgcaa atccagagta ctctgaagaa tcaaaaccag ccatttctaa tacattagga
    19141 tgtaccattc cagcacctaa aatttcaatc catcctgtgt gtttacacac attacaacct
    19201 tttcctttac atttaaaaca tgacacatca acttctacag aaggttcagt gaatgggaag
    19261 taacttggac gtaaacgaat ttcacgatca gcaccaaata atttcttagc taacaattct
    19321 aaagtacctt tcaaatcact cattttaacg tttttatcaa caactaatcc ttcaatttgt
    19381 gtaaattgat gactatgtgt cgcatcatca gagtcacgac gatacacttt accagggcaa
    19441 ataattttaa ctggaccttg accatgacgt gattccatcg tacgtgcttg cactggtgat
    19501 gtatgcgtac gtaataaaat ttcatccgta atatagaaac tatcttgcat atcacgtgca
    19561 gggtgtgatt taggtaaatt cagcatttcg aagttataat gatcttgttc aacttcatat
    19621 ccattcacaa tttcataacc taatcctaag aataagtctt caatttcttc tattgtacgt
    19681 gttaatggat gctttgaacc gatttcaatg tgacgacctg gtaatgatac atcaattgtt
    19741 tcttcagcca attgcttatt taatttttct ttaactaaca tctgttgtct ttcatctaat
    19801 tcattttgaa ttgtttgacg caattcattc actttttgac caaacgcagg tttctcttca
    19861 ttcggcaaat ccttcatcaa tttcattagt ccgctaactg accctttttt acctaagtat
    19921 ttcactttaa cttcttgcag tgcacgttca tcatttgctt cattaatatc tacaagcgct
    19981 tgttgtttta actctgacat tgtttgttgt tcagacatat gtcactgacc tccctattcg
    20041 aactattcat tttctattta tcatatttgt tgtttaacta aaaaatgact ttcatcctct
    20101 taaaaaataa gggacgaaag tcatttccgt ggtaccaccc aaatttatgc ctaagcatac
    20161 acttgtttaa tattgataac ggattgtctt tccgacttaa atctctttaa gtaaccaaaa
    20221 aggtgaaaag actttaaata attggaataa cttgcagtca cgattattct ccctaaaaca
    20281 attatttata taaatgtcca tgccttttta ttggtttata ttcaattgtg tatgcaatca
    20341 cgtagaaatg ttctgttaaa caattaatta taattatatc ggaaaacttt caacacggtc
    20401 aacctttcaa atgataaagt aaaatactac ctgcaatcgc tacatttaaa ctttcagctt
    20461 taccataaat aggtatgatt aaattttgtg tagtatgtgc taataattca ggattaacac
    20521 cttctccctc attacctaat agtaatgcaa aagaatcact tgaagtaact tctttatatg
    20581 tcactgcgtt ttcaagtgct gtaccataaa caggaccatt aaattgagta ataaacgtat
    20641 cgagatcttg tgtcataact ggcaaatgga aaacactacc ttgactcgct cgcaacactt
    20701 tatcttgata aggatctgtc gtacccttct ccattattac agcatccatt ccagcagcat
    20761 ccgcagttct aattaatgtg cctaaatttc caggatcttg aacacgatcg ataagcaata
    20821 cttgttgtgc tttagaaata tcatattgtg gcttctcaat gatggcgaaa aacccttgtg
    20881 gtgtcactgt acctgataaa gattcagcaa ctttcatgtt tatttcaaaa acttcttgcg
    20941 cgtatgcgat aatttgctga tctaatcttg ccggttcaat tgcaaataat tgtgtaatta
    21001 caatcccact ttgataagct tcttcaatta aatgcacacc ttcaattaaa gctaatccag
    21061 ttttatccct ctcacgtttc ttttttagct tgttcgcttg tttaattcta ttattttgtg
    21121 cagaagtaat ttgttccata gatagctcct cgctttattt ttaaaaataa aaatattaat
    21181 cattaataag atgaaaacat ttgattgtat agttaatatt aattaatcga ttttatcact
    21241 caaattattt caaattgtat aaatttcttt tatcgatact actactataa atcatacgcc
    21301 ccaaaatatc attattaatt cttttcttct tcaaaataaa tcaaaatcat ataattgatg
    21361 attattttca aagcacattc aaatcaaact acgttttagc aatttgttgt tagcatgttt
    21421 gtgttcatta aaaaaacgac catcatcggt atcatgtatg gtcgttacta aagttaacaa
    21481 taccaatagt cataacaagt actgcaacct ctttaaattc aattatttca tgtaactata
    21541 gcctatatca tatgtaatta ctttgttatt gataatcggg ctactttcat cttcattttt
    21601 acttctaaca tgtttatgtg ctgctttaaa gacatcagat tttaaccaat ccgtaaaagc
    21661 ttgctttgat ttccaaactg ttaaaatttt cacttcatca aaatcttctt gttctaaagt
    21721 ttgtgtaaca aacatgccat caaagccttc taatgtttca atcccatgtc tcgtgtaaaa
    21781 tcgttctata atatcttttg ctgttccttt tgttaacgtc agcctatttt ctgccataaa
    21841 tttcataatt atcctctttt ctgtttaact taccttaatt atttttgtga caacaacaat
    21901 tcttttcgtt gtttcactat atgcatcttc gcacgttgat aaagtcatta ttttatcttt
    21961 taccgttaca ttaacatctg agttaatcac agatttacgt tttgtctcat ctaaaaattg
    22021 ttgataatct tgatcatttt caaaatctgt acgtatgtaa ttatctttag tagtagtttt
    22081 atatgcacta aatacttgca attgatattt accatattta ttgtcaaatt caattgtctt
    22141 gtgtttttca taaaacgatt gctttaaata atcttctaac acatcaaaca tcgtattatc
    22201 accgacatgg tgcccgtata aaatagtatt atgatttaaa ttcttcaatt catttctaaa
    22261 atccataaaa atactacctt tacgtcgatg ttctcgctca aaatctaaat ttaaataatc
    22321 gtgatttgtc ttaccttgta gtactggata attcaatgat gttcctgata attttatcca
    22381 tccaacaatg tctttattta ttttttcaag tgattcaaat tgtggtctca catgtgcttg
    22441 atgtttgctc atcagcattt gaaatttttg ttgtaatttc tcataatttg cgcgttcttg
    22501 cttgtcttca atatatgttt gaacaatttt gtaaccaaaa atgataataa ttacaaccaa
    22561 taaaatttgt acaatagtta aaaatcgctt cattctcata aaaatcctct tttattaacg
    22621 acgtttcttc agtcatcact aaaccagttg ttgtaccgtt ttagattcga tttcgttgac
    22681 tttgacaaat taagtaaatt agcattggac caccgacaat cattaaaata gcattggctg
    22741 gaatttctaa aggaggctgt atcactcgcc ctaataaatc agccactaac aatagccatg
    22801 caccaataac tgtagaaaac ggaataagta ctctgtaatt gcccccaact agctttctaa
    22861 ccacatgtgg cacaataata cctaaaaagg ctagttgtcc aacaatcgca acagttgcac
    22921 ttgctaaaaa tactgctaat aaacctgtta accatctgta acgatcaata ttaaaaccga
    22981 tacttcgcgc ttgtatgtca tctaaattta gtaaattcaa tttaggggac aatagtaatg
    23041 ttaatattaa tcccaataat gctgatactg ctattatgta tacgtcgctc catattttca
    23101 ttgttaagcc ttgaggaatt ttcattaaag ggttttgagt taaaatttct aaaacaccat
    23161 ttaataatac gaataacgca acgcctacta atatcatact tacagcattg aatctaaatt
    23221 tagaatgcaa caatataatt attaaaaatg gtattaaacc tccaataaaa cttaataatg
    23281 gtaagtaaaa gtacaattgt ggaataaaca acatacaaag tgctctcatt ataagtgcac
    23341 ctgaggaaac gccaatgata ttcgcctctg ccaaaggatt ttgtagtgct gcttgtaata
    23401 atgctccaga aactgctaac attgcgccaa ccatcaatgc aattaatata cgtggcaatc
    23461 gcaaatcaat gattgaatcc actgcttcat tgctaccagt tgtaaatttt gtaattaggt
    23521 cattaaatga caatttaatt gtaccggtta caaacgaaat ataagctgtt gcgattaaaa
    23581 tgactaacaa acataaaaat agtagtttct ttttattttt aatcatcata tcaactcact
    23641 attttttatc cttataaaat aaatcatata attgagtcat tgccttatca gcatcaacat
    23701 ttgctgtaat accgaatggc acttcctcta agtcataaac atgattattt ttcactgctt
    23761 taaaatgttt ccaaatatca ttttgtttaa attctttttg aaacattttc ttaacttctt
    23821 caggcattcc gtgtggtaat cgtaaaataa tatctggatt gatattcaac aaattttcag
    23881 tattagacga aatatattga cgatctttca ctttaataac attttctcca cctgctattt
    23941 taactaaatc accaatatat gatttatcag ttgctactaa atagctaccc ggtacaccca
    24001 ttaatattaa tactttggga tgtttctttt gtttagctgc tttattttca attttttgct
    24061 ttacagaatt taaatggtca tttaattctt ttgcttgtgc tttacgatta aattgatcac
    24121 ctaattgtgt aatcgacttt tgcatccctt ttaaactatc aaaatcataa aagtagcctt
    24181 tcatatttaa ttgtttgtaa aatggttgca tttcatcttt aatcgttgac acactcaaaa
    24241 catgtgttgg ttttaacttt ttaacagctt caacattcgt ctccattggt tgaccaattt
    24301 ccggtacatc tttataacga ttaggcaatg tcttatatga cgtgggtttg ccgacaattg
    24361 gtaaatccaa tttgtccaat gtcattgtca atgcaacagt tgttggtacg attctgaatt
    24421 cgccggattt agttgattct tgagaactgg aagattgaca gctggttaag ataacgacgc
    24481 ttatcactaa aatggttaaa tactttatga ttctcaaact tggttacctc tctttctgct
    24541 gtataaatgt gccgaaataa gtaaaatggt tgctaacaca atgccagctg caaataacat
    24601 atattgcttt ttatgatttg gttcatcaag ttctttaata tcactagata attgactact
    24661 attcttgcta tcatcatctc tattaaaagc tggtaacgct ttttcctttt caactacttt
    24721 tttcttttcc ttttttgatt tttcatcttt atgtttcggt tgctctttgt gatcattttc
    24781 aatctttttt actgttggag ggctattttc aacttttaat gtacctgatg ctttaggtcg
    24841 ctcattttca atctttttat tactgtcttg ttgcacttta tctttttgta cagaaatatc
    24901 atttaatcct ttttcagtaa aaaggctagt acgtctttcc ttaatatctg atttttgact
    24961 atcttttttg tcatcaatat cttttttaag ctgatcttga ttattttctt gattttgatg
    25021 ttttgaaggc acttcttttg catcatcatg ttgtactttt ttctctttaa aatgaataga
    25081 atacaccatt tctttgttat ctagatcttt ctttgcagcc aacgttttaa catgcatatt
    25141 gattttatcc atatgctgtt caattaaaat tcttactatt gtgtcatcct tttcatcttt
    25201 tataacaaaa ggaaattctt catttttgtc attttgaaaa acaagttgtt ctttatcaat
    25261 ttgacctttt agctttactt gtaagtaaat tttaccagtc atatctgaaa tcaattgcgc
    25321 cttttcgtca actagttgtt gttcctcagt tacattttta ttttcttgat taaaaattag
    25381 taactctaac gctttattct cttttatctt ttcatcaaaa attgatgaag ttaattctgt
    25441 tttatctttt gcttcaggtt gattaaattt ggctgatgga tattttatct catcagccaa
    25501 cacgctacca atcattgttg tatatgtaag tatacctaag ctaataatag ctataataga
    25561 ttttgtagca atttgtttaa catttctcat tattccacat tgcctttaga tttttttcta
    25621 acaaacaatg tgatcgcgat taataatgct aaggatgcaa ctggtattgc atatatatat
    25681 gcaggtgtac cagcatttgt ttgtggattt tctactttgt ctttattcga actgttagat
    25741 tcactttgac cagtagtcgc tccatcagat cctttgtcac tacctgaagc agaattttta
    25801 tcatctttac ctggtgcatt agcacctgct acatcagttg gtccattaaa tttatatgta
    25861 atattgtaat gatggtcata tttgaatggt tttccgttca ctttttcatc gatataaaca
    25921 tcaatttttc catctacttt accgtttagc ttacttactt caaattcaga agttcgttca
    25981 tctttggcag tatttttact aataattttt tctttatgcc cttctatact cattcctgta
    26041 atccaatgac tgtggtttac agttatttga acatacaatt taccattttt cttaatgtac
    26101 tttgccggtt tattaaaata gtcattagca atcgacgtgt cattggtatt gtatttgtaa
    26161 acctcataat tcaaagtgcc gctatccgcg gcatttgcag aattactgaa tgtcgcgatg
    26221 atgataatta atgctaaaat cgttgtatta aaaactttta aaatattttt caaaacataa
    26281 tcctcctttt tatgattgct tttaagtctt tactgaaatc ataaataata atgattatca
    26341 ttgtcaatat ttattttata atcaatttat tattgttata cgaaaataga tgtgctagta
    26401 taattgataa ccattatcaa ttacaatggt taatcatctc atataacaac acataatttg
    26461 tatccttagg aggaaaacaa catgacaaaa cattatttaa acagtaagta tcaatcagaa
    26521 caacgttcat cagctatgaa aaagattaca atgggtacag cgtctatcat tttaagttcc
    26581 cttgtataca taggcgcaga cagccaacaa gtaaatgcgg caacagatgc tacgaacgca
    26641 actaataatc aaagcgtaca agtttctcaa gcaacatcac aaccaattaa tttccaagtg
    26701 caaaaagatg gatcttcaga gaagtcacac atggatgact atatgcaaca ccctggaaaa
    26761 gtgattaaac aaaataataa atattatttc caagctgtat taaacaacgc atcattctgg
    26821 aaagaataca aattttacaa tgcaaacaat caagaattag caacaactgt tgttaacgat
    26881 gataaaaaag ctgacactag aacaatcaat gttgctgttg aacctgggta taagagttta
    26941 actactaaag tacatattgt cgtgccacaa attaattaca atcatagata tactacgcat
    27001 ttagaatttg aaaaagcaat tcctacatta gctgacgcag caaaaccaaa caatgttaaa
    27061 ccggttcaac caaaacctgc tcaacctaaa acacctactg agcaaactaa accagttcaa
    27121 cctaaagttg aaaaagttaa acctactgta actacaacaa gcaaagttga agacaatcac
    27181 tctactaaag ttgtaagtac tgatacaaca aaagatcaaa ctaaaacaca aactgctcat
    27241 acagttaaaa cagcacaaac agctcaagaa caaaataaag ttcaaacacc tgttaaagat
    27301 gttgcaacag cgaaatctga aagcaacaat caagctgtaa gtgataataa atcacaacaa
    27361 actaacaaag ttacaaaaca aaacgaaacg cctaaacaag catctaaagc taaagaatta
    27421 ccaaaaactg gtttaacttc agttgataac tttattagca cagttgcctt cgcaacactt
    27481 gcccttttag gttcattatc tttgttactt ttcaaaagaa aagaatctaa ataatccatc
    27541 gtcacactca taacttaata tattttttat tttaaatttt atttaaccta tgtcttagat
    27601 atttcataat ctataacata ggttattttt ttataaaata accttgcaat taactaccat
    27661 ttcaatgtac aatacaagta attaattgat aatgattatc agttgataat atacaattag
    27721 gagttgtttc tacaacatga acaaacagca aaaagaattt aaatcatttt attcaattag
    27781 aaagtcatca ctaggcgttg catctgtagc gattagtaca cttttattat taatgtcaaa
    27841 tggcgaagca caagcagctg aagaaacagg tggtacaaat acagaagcac aaccgaaaac
    27901 tgaagcagtt gcaagtccat caacaacaac taaaaaagct ccagaagcta aatcagtagc
    27961 caatgctgtc tcagtatcta ataaagaagt tgaggcccct actcctgaaa caaaagaagc
    28021 taaagaagtt aaagcctcta aggaaacaaa agaagttaaa ccagcagcaa aagccgataa
    28081 caatacatat cctattttga atcaggaact tagagaagcg attaaaaacc ctgcaataaa
    28141 agataaagat catagcgcac caaactctcg tccaattgat tttgaaatga aaaaagaaaa
    28201 cggtgagcaa caattttatc attatgcaag ctctgttaaa ccagctagag ttattttcac
    28261 tgattcaaaa ccagaaatag aattaggttt acaatcaggt caattttgga gaaaatttga
    28321 agtttatgaa ggtgacaaaa agttaccaat taaattagta tcatacgata ctgttaaaga
    28381 ttatgcttac attcgcttct ctgtttcaaa tggaacaaaa gccgttaaaa ttgtaagttc
    28441 aactcacttc aataacaaag aagaaaaata cgattacaca ttaatggaat tcgcacaacc
    28501 aatttataac agtgcagata aattcaaaac tgaagaagat tataaagctg aaaaattatt
    28561 agcgccatat aaaaaagcga aaacactaga aagacaagtt tatgaattaa ataaaattca
    28621 agataaactt cctgaaaaat tgaaagctga atacaagaag aaattagaag agacgaaaaa
    28681 agctttagat gaacaagtga aatccgcaat aactgaattc caaaatgtac aaccaacaaa
    28741 tgaaaaaata actgatttac aagatacaaa atatgttgtt tatgaaagtg ttgagaataa
    28801 cgaatctatg atggatactt ttgttaaaca ccctattaaa acaggtatgc ttaacggcaa
    28861 aaaatatatg gtaatggaaa ctactaatga cgattactgg aaagatttca tggttgaagg
    28921 tcaacgtgtt agaacaatta gcaaagatgc taaaaataac actagaacga ttattttccc
    28981 atatgttgaa ggtaaaactc tatatgatgc tatcgttaaa gttcatgtaa aaacgattga
    29041 ttatgatgga caataccatg taagaatcgt tgataaagaa gcatttacta aagccaatgc
    29101 cgataaaact aataaaaagg aacaacaaga taactcagct aagaaggaaa ctactccagc
    29161 taagcctagc aaaccaacac catcacctgt tgaaaaagaa tcacaaaaac aagacagcca
    29221 aaaagatgac aataaacaat caacaagtgt tgaaaaagaa aatgacgcat ctagtgagtc
    29281 aggtaaagac aaaacgcctg ctacaaaacc agctaaagct gaagtagaat caagtagtac
    29341 aactccaact aaggtagtat ctacgactca aaatgttgca caaccaacaa ctgcttcatc
    29401 agaaacaaca aaagatgttg ttcaaacttc agcaggttct agcgaagcaa aagatagtgc
    29461 tccattacaa aaagcaaaca ttaaaaacac aaatgatgga cacactcaaa gccaaaacaa
    29521 taaaaataca caagaaaata aagcaaaatc attaccacaa actggtgaag aatcaaataa
    29581 agatatgaca ttaccattaa tggcattatt agctttaagt agcatcgttg cattcgtatt
    29641 acctagaaaa cgtaaaaact aattaatcgt ctttatattt aattattaaa ttaacaaatt
    29701 ttaattggtg gatgaggtag cctgttacct cgtccgccaa ttatttttgc aatataaaaa
    29761 gtcccactta aaacaatcat tttaagcggg actttttata ttgagtaact aaaattattt
    29821 agctgctact tcttcgccat tgtaagaacc acagttttta catacacggt gtgataattt
    29881 gtattcgcca cagtttgggc attcagtcat acctggtact gaaattttga aatgcgtacg
    29941 acgtttgttt tttctagttt tagaagttct tctttttggt actgccatga tatatcctcc
    30001 ttagattata aacgaaaaat actaaatgtt agtttaatta acaacattat atcattaatt
    30061 aaactactta ttgctcttta tcatataatt gttgtaattt ttgaagcctt ggatcaactt
    30121 gtcgtgattc tgaatcatct tgttcttgct gtttagcaag ctcatctaat tgatcctcat
    30181 cgattacttc ccaaccatta cctactgtca acatttggtc actttgctct gaataagctc
    30241 tcattggttt ctcaataata actatatcct cgacaatatc atgaagatta accataccat
    30301 ctttaataat gtgatagtgt tcatctacat catcttgatc atcgttatac tgattatacc
    30361 cttctaaatc aaatacttct gtagtagtta catctagtgg gactttaact ggtacaagag
    30421 tacgtgcaca aggcattgta tacgtaccag taatgtgaat atccgcaacg acttctgttg
    30481 acttaatggt taactgacct tggattgtaa ttggagataa atcaattaaa tctaatgatt
    30541 cttttaaatt gtcaaaactc accgtttgat caaattcaaa tggcttacct tgatatttcc
    30601 ttaattgcgt aattgaccat ttcatatggc ttcacctcta acaagcacaa aatttatttt
    30661 aactttctaa gaacaagttg tcaagatttt ttcttaacat atcatgttct gttacaatat
    30721 taataatgaa taccgaaagg acgaattgca aatgaaaagc gttggcttaa tcacagaata
    30781 taatcccttt cataatgggc atcaatatca tattaatcaa tctaaaaaac ttacaaatgc
    30841 tgacgttact attgcaataa tgagtggtaa ctttgtcatg cgtggcgaac cagcaatcta
    30901 tagtaagttt actcgtgcaa aaatggcatt atcaacagct gatttagtta tcgaactacc
    30961 agcaactgcc agtttatcat ctggcgatca ttttgccgaa ctagcagtta aagtcgcaga
    31021 ttatatgaat gtcgatacaa ttgcgtttgg tagtgaaaat aatgatatca aaacattaaa
    31081 gcaattagct cacagcatta atgaaattga acaatccgaa tccttttcac aaaaagtaaa
    31141 agaaggtaaa agctaccctc gtatcattag cgaattatta gagcatcatg aagcactagc
    31201 aagtcctaac aacatacttg gtattagtta tctgaaagca attgctaaac atgctaaaaa
    31261 catcaatgca atttctatta agcgagaaaa tgctcaacat catgattcat taattcaaca
    31321 tcatcagttt gcaagtggta catctattag aacatctatc attagtcaag atgatcattg
    31381 gcatcatgtg gtacctaaag atattcaaca cctgtacgta acacctcata taacattaaa
    31441 tcaaatattt ccatatttga aatatcaaat cattgctatg actacagagt cattaaagaa
    31501 tatttatact gttactgaag gttttgaaaa tcgtttaaaa tctaacatta atgaagcaac
    31561 tgattttcgt cattttgtaa aatcactaaa aacaaagcgt tatacatata cgcatatcca
    31621 aagactgtta atgaatgtat tattaaacat taaacctaca gatgttacga gaaacattca
    31681 cgcagttaaa gtattagcga tgaacgaccg tggcagacaa tatttaaagc atttaaaaac
    31741 agcgttccca gaaagacaat atataaccaa tattaacaaa agcaatgcac attacttcac
    31801 aaatgaaatc aaagcaacac acatatacaa tgcaataagc gggcaacaac aaacagattt
    31861 caacacgcct gtcatacagc aatatcgtta acaaatgaaa acagtatttt aggattgtaa
    31921 accatgataa acctaaaata ctgttatttt tattacttaa atttcttctt caatgccttt
    31981 tcaacataag gtggaacgaa ttctgaaata tctgctcgat aagctgcaac ttctttaaca
    32041 atacttgaac ttataaatga ataattagta ctagacatca tatataacgt ttcaatttca
    32101 ttgttcaact ttttattcat tgaagttaag cgtaattcat attcaaaatc actgactgct
    32161 cttaaaccac gtatgattgt tttagctcct acttgttcac aataatcgac tagtaaacca
    32221 ctaaattgat gaaccttgac attaggtaaa tgtttaacag attgttcaat taaatccata
    32281 cgctcttcta aactaaacgt accttctttt ttactatttt taagaacaca gacatgaatt
    32341 tcatcaaatc tatctgtact tctctcaata atgtctaaat gaccataagt aatggggtca
    32401 aaactacccg gaatgaccgc tattgtatgt tccatgctat tctccctttt ctaataacaa
    32461 tgtgtctgtc aacccataat ggtaacgttt aatcatatta aacggttgat agtctatttc
    32521 ttcatgattg ctaaattcac aaacgatgat accattttct ttcaataaat taaactctga
    32581 aattagtttt aaagctttat caatgagacc tttattataa ggtgggtcta agaaaatgac
    32641 atcaaattga atataacgtt ttgacaatgc ttttaaagct ctatctgcat tatttttata
    32701 aacttcagat tgtgcctcta aatccaaatt cgcaagattt gatttaataa cttttacagc
    32761 tttaaaattt tgatcaacaa agataacctt atccatacct cgagagagtg cttctattcc
    32821 aagcgccccg cttcctgcaa ataaatctaa acctatacct gacacatcat ataaactatt
    32881 aaagatacct tctttaactt tatccatagt tggtctcgta ttacggcctt ccatactttc
    32941 taaagcttta cttttatgtt tacctgcaat gacgcgcatg ttgttcacac ttccaattca
    33001 tttagttatt taatataatt tattgagaaa aaggagaatg ataaaccaat gaaacaaaca
    33061 tttattacac ttggtgaagg tctaacagat ttgttcgaat tcatgacgat gattgaatat
    33121 aaccatcaac gtattgataa aattatctat tttcattcac cacaagctga aaataaaaag
    33181 tcatctgtag caatcattat gaatcctaca actggcaatc atttccaagc attttatatc
    33241 atgattaacg ctattaaata tccatatcca gattcaaata aaaagtttca aatgattaat
    33301 gattgtgctg aaaaattcga cataccaatt ttaggtatcg atgtacagcc ccctcaagca
    33361 tttcatgatt tatcgttata ttataattat ttaattagtg tgttaagact ccaaaaatgg
    33421 ataccagaac ttcaataata ttaattatat atttcgtgtt tctctttttc gtaagttttc
    33481 tttaagtatt tatatggtga gccatcaatg tgtctaacgt atttaagttg cattagttta
    33541 tgtacaattt gatcaacatc ttgctcattc acatacataa ttacaaattt acgatctcta
    33601 tttgaatgaa cgatatgtcc atattttcgg atttgtcgtt catgtttcat atgttttaaa
    33661 taaactacaa tactagttct tgggattaaa ttcatttaaa cctcactcca tttattttca
    33721 gcattaatca tatcatgtct ttattaattt taaaatatta agctacctta attatgtctt
    33781 atgtaattgc tcgataaatg tatttagatt atattaaaca tcattatatt aattcaattc
    33841 tcaaaagcat cactatatga ttaaccacac attttgctgt agtcagaact atgaatttgt
    33901 taagctataa aagtaaacga ggaggaattt ttatgaaaaa agtaaacaga aacattttaa
    33961 atacggtact agtcggtgct ggatttgtaa gtagtctgtt aatgataaat aaaaataaag
    34021 tgattactaa aaagcaaacc atccctgctt ttttcaaagg caatgcacca tatatatttg
    34081 cgcatcgtgg tggtatggct ttaagaccag agcaaacgca acttgcattt gactatgcta
    34141 aacaattagg cgttgatggc tttgaaacag atgtgagact cacaaaagat caacagctta
    34201 tcgtttttca tgacgcaact gtcgatagaa cgacgaacgg ctctggaaaa gttagtgtcc
    34261 atacattagc agaattaaag aaattagacg cagcttatca cttcaaagat atcaatggac
    34321 taacaccata tcgtggtcat gcacacacag ctatactaac cttcgatgag ttgctgaagc
    34381 aatatcctga tatgtatata aacgtagact taaaagatga tcctgaatcc tatgaaggct
    34441 caatcgcacc acaaattatg tttgatacaa ttgcagagaa tcaagcattt gatcgtgttc
    34501 ttgtgacaag tttttataaa gaacaaatcg ttcgttttaa taaaatcgca caaggatccg
    34561 ttgcaattgg tgctagccaa caggaagtta cagaggcatt tttgaaatat catttattag
    34621 gtggcaggta ctaccaacca ttagcacaaa cgttccaaat gcctactcat tttaaaggca
    34681 ttgatttgac ttcatctcgt tttatcaaat ggttaaatga tatgaatatc attccaggct
    34741 attatggcgt aaatagtata gatctgatga atgatttata ccaaaaaggt gcacacacga
    34801 ttgtgacaga tcgaccagat ttagcacaac aattcaaaca aacaatccca aacaaataaa
    34861 aacatcgagt cagatgctag ttagtggttt caataaccat ctttttctga ctcgatgttt
    34921 tttataatga acaattacag gatccgcccg tcgcacaacc actatgtgat gtttgaaaga
    34981 atggattacc tgtttctatt ttgacatgtt ctgaaacagc gtacgcaatt ttcgtaacaa
    35041 cttcatcaat taaatgttga agtgccattt ccctagcttt gtaatgcatc acaacgtcaa
    35101 gcatctcata tgctcttttg cgttgtcttg tctctaacat gactttctta taatcaggat
    35161 gatatttccc gaaacgcatt acttcatcat atttctcttt tgattttaaa aatgattgat
    35221 aaagtaaatg ggcttcgtca ttgttttcga gcgcctgttt agcttgctca aatgatgcat
    35281 aaatatctga tgcaactatc atatcagcta gttcatcaat atcatctaat atcgctagtg
    35341 aagcttcatt aatcataaat acccctcatt gttatttttc taagaaaatt agtgaataat
    35401 aatctctcat tacatctcca cccatctcat cgtattttga atttaataga cgagatcgat
    35461 gtatatctga attcatccaa ctatggatta atgttggtac atcattaaac gcataaccaa
    35521 cattttgagc agttgtttta taagtaacgt gatttttatc taattgccct cttaatgcgt
    35581 cctctgtaaa ttcaacacta tcagaaccat tagaggtcgc ttcatataag ttattagatg
    35641 caatatgtgc taaatcgcta ttgattttca atggttttaa tccttttaat tttctcattt
    35701 cattcgttac ttcataaaga gaaattaatt gatttggatt ttgctcaact ggacgcttat
    35761 tatgctcttc tgacgtaaaa ttagaattta atcgataagg ttcaatatct gctaacattt
    35821 cttttgttaa aaatcgcaca cttagcacct ttttcgattg ttgatcagaa tacacttgtg
    35881 cgtatatgtt gccatattta atcagtgttt gtgtttttaa atcttcatct gaaagttcaa
    35941 attcatattt tttaccatca actttaaagg acggttctgg attaatactt gtatgattaa
    36001 aaatttctgc agaatgttgt cctattttta acggactaac atttactttt tcacctgtag
    36061 catacactga aacgatttct tcacgtttag ttgaaacaat gtaataactg tttttgtctt
    36121 taaacacata atttttgtaa ccatctctaa aaggatagac acgatctgct tgtccaaatt
    36181 tagaagtaag cactttaata tctttaccta cccatgtacc aacaccttct ttaggctttg
    36241 gattttcagc atggttattt gatttattca cctgttcaga tctatttact ttattacctt
    36301 tattcgggtt ctctaataca tcaaatttta atctcggtga ataaaaaaga tatattaaaa
    36361 atccaactaa aaataataca ccgacgactc ttataattaa ctttttcatc aatcaaccac
    36421 ctaaaaagta ttaatactat tgtaaaaaac aacacattaa ttagcaaatt ttcaacactg
    36481 acataactgt gtcacttcga tgaaacaaaa tcattttcag gcataatgtt aacatcataa
    36541 agattttccc tacactctat atcataacct atctttatgt tttcaggttg aatttcatta
    36601 ttaggattaa aatatgtata aaaaaacgga cagttaaggt atcaaatgaa taccatcaac
    36661 tgtccgacta ttcttcatca aaaaacctga taaaacaaat agccttatca gattagtatc
    36721 atttgtataa gcgaattaat gaccaaatgt tgctttaatc agtgatgttg tttctccacc
    36781 tggatataat acatataata ataagtaaac tgctacacct gtaattgcag tacagaacca
    36841 tataactgaa gcgaatggcc cgaatttgcg gtgtacatta tatttatctt taaatgcagt
    36901 aattatttga actagaccta gaataccacc aattgttgct aaattaatgt ggaaaaataa
    36961 gaaaatcgta taatatttct taattgatgc tggtccgcca aatgctgtat taccgataaa
    37021 aatcgttcta cttgcataga ttaagaaaaa agttaaagca aaaacagctg cagctaacat
    37081 aacatttttg tgtttattta tttccctttt ccaaataagt ctccaaccaa tggcaattaa
    37141 aattgcacta atgacaatac atgtcgtact aatcgttggt aaaattggaa cgcccatatt
    37201 tttcatccta acttaattaa tctagatcaa agtaagtaat gaaacaatca cagctaacac
    37261 gaaaaagatc actaaataat ttagtgaata tataaacatt tgtgttgccc attttgtttg
    37321 atctgaattt ttcttaaatg ttgttaaacc taatgcaatc catcctaaat ttaataatgt
    37381 agctaacact acgaatacga cacctaaatt tattagtaat aaaggtactg gcaataaaat
    37441 aatcaaccag ataaacatac tgacacgtgt acgtttaaag cccttaactg atggtaacat
    37501 tggaatattt gcaagtgcat attcatcttt acgtttaata gctaaggcat aaaaatgaat
    37561 tggttgccaa caaaatacaa ctaaaaacag cgcaatcgct gttaaactaa tttgtccttc
    37621 aattgcaacc catccaatta gtggtggtac tgctccagga aaactcccaa tcactgtgtt
    37681 ccatgttgta tgtcttttag accatattga gtaataagac acataaccta caatccccat
    37741 aagaccaagt acgcctgatg gtatattcaa taaaaacaaa caaatttctc caactaacat
    37801 cataccaaaa cttaatagta ataaattttg atctgtaatt ctattattta cagttggtct
    37861 attttgttta ctaggcataa tacgatcaat atcttggtcg taataattat ttaacgcaca
    37921 tgcgccaccc ataattaaag tagatcctaa tagcattaat aaaatttgag gtattgatga
    37981 taagaaggaa tgatttgtca ttacaactgc tagccatgcg cccgcaaaag ctggaattaa
    38041 gttaccttga acaagtccca ttttaattat ctgttgcaat tctttgaagt taactctgct
    38101 aatattttgt gacaaagtat gctctttgct cataatcccc ctccttaaat ttgttcatat
    38161 aagattatga tatcttagat tgcataaaaa gactaggttt aataaaatta aattgtgaca
    38221 aattaacgac aagagaaaat gtcaattttg tgacacaaat aacatttaat ttattgctat
    38281 aatgtatatg ttagaaaatt ttaataagta gaatcatgca tctaaaagag attaatattt
    38341 aagcttcaaa tttgagtaaa cgtggattac ataattaccc caataaaaaa atcattacga
    38401 ttaaggtctt tttatgtcgt ccacttacaa tacttgtaaa attaaatcat atttcctgcg
    38461 ttggatccca tcttttcata tcctacaatc aggtcaattt atagtatcat ctcaaatccg
    38521 gctattaatt ctaattctca gtgatgcgtt tttttatgat ggggtgtata aattgtttgg
    38581 caaaaagaat ttaaaatggt taggtgtcgt agcaacgtta atgatgacat ttgtacaact
    38641 tggtggtgcc ttagttacca aaaccggatc agctgatggt tgtggttctt cttggccact
    38701 atgtcatggt gcgttgattc cagaattctt tcctattgat acgattattg agttaagtca
    38761 tagagccgtt tcagctttgt ctttattaat ggtcttatgg ttagttatca ctgcatggaa
    38821 acatataggc tatattaaag aaattaaacc tttatcaatc attagtgttg gattcttatt
    38881 attgcaagca ttaatcggag ctgctgctgt tatttggcaa caaaacgatt acgttttagc
    38941 attgcacttt ggtatatcat taatcagttt ctcatctgta tttttaataa cattaattat
    39001 tttctctata gatcaaaaat atgaagctga cgaattatat atcaaaaagc cattaagacg
    39061 tttaacatgg ttaatggcaa tcatcattta ttgtggtgtt tacactggtg cactagtgag
    39121 acatgcggat gcaagtttag catatggcgg ttggccattg ccattccacg atcttgtacc
    39181 acattcagaa caagattggg tccaactcac gcatcgtatc atggctttca tagtgtttac
    39241 gattattatg attacttata ttcacgctgt taaaaattat ccaaataacc gtactgttca
    39301 ttatggttat acagctgcat ttatacttgt tatattacaa gttatcacag gtgcattgtc
    39361 tattatgaca aatgttaact tgataatcgc actattccat gcattattta tcacttattt
    39421 atttggtatg acaacatact ttatcatgct aatgttacga tcagtgagaa gtgacaagca
    39481 ataacaaaaa agcaaaccgt aattttaatg ccacgcccat taaaattacg gtttttttat
    39541 atcaatattt aaaattaaac ctaagacaag taaaaacgag attacacgtc aattgttgtg
    39601 taatctcgtt ttattttaat cattttagtc agttgctttt tcaatttcga ttaataaatc
    39661 gcctgtcgct attgtgtcac cattatttac agttacttgt ttaatcacac cgtcaaatgg
    39721 tgcttgaatt gttgtttcca ttttcatagc ttcagtaatt agcaatggct ggtttgcttt
    39781 cacagattca cctacactaa ccttgacttc agttactgaa cctggcattt gcgcaccgat
    39841 atgacttgga ttacttttat ctgcttttgg cttaacgttc gcatttgtat gcacattttc
    39901 atctttaatg taaatacgtc tcgcttgacc attcatcgca tagtaaatcg ttctattacc
    39961 attttcatct ggttcactaa tcgtttctag tttaataatt aatcgtttac ctttatcgat
    40021 ttcgatttct actgtttcac cattacgcat tccaaagaag aatgtaggcg tatcaagtaa
    40081 cgataagttt ccgtattgat ttctagtttg aatatattgt tcatatactt ttggatataa
    40141 tacataacta ataatatctt gctccgtaac aggaccttgt tgctcttctt caagcaactc
    40201 acggactttt tcaaaatcaa ctggctctag atattcacct ggacgagctg ttaatgcttc
    40261 ttggcctttt aaaataaccg cttgtaaatc tttattaaaa ccatttacag gttgtcctat
    40321 ttcacctttg aagaacgaca ctactgattc tgggaaatct aatttataac catctgtaat
    40381 cacggattgt tcatcaagat cattttgaac catataaagt gccatatcac caactacttt
    40441 agacgatggc gttactttta cgatatcacc aaataagaag ttcactctgc gatacatatc
    40501 tttgacttca tcaaatcttt cgcctaaacc taaactttta gcttgttgac ttaaattcga
    40561 atactgtcca ccaggcattt catgttgata aatttcagta ttcggtgatt tgatatcact
    40621 ttcaaagtct gaataataag tacgtacagt tgaccaataa tgactaagtg actccatacc
    40681 ttcaatatca gttctaaggt gacgtgggaa gccatttaat gcataatata acgaattggc
    40741 gcttggctga cttgttaaac cactcattga agcaacagca gtatcaatga tatcgacacc
    40801 agcatctatt gcttgtttgt acgttaataa accattacca cttgtatcat gagtgtgaag
    40861 atggattggt aaatctacag ctgcttttaa ctcaccaatc aattcgtaag cagctttagg
    40921 ttttaataag cctgccatat ctttaatcgc taaaatatgg aaaccttcac gttctaactc
    40981 tttagctagt ttgacataat actctaaagt ataaatgttt gatcgctcag gatttaaaat
    41041 gtcacctgta taacaaatag taccttctga gattttgccc gcttcttgta ctgcttcatt
    41101 ggcaactttc atttgatcta cccagtttaa tgaatcgaaa attctaaaga catctatgcc
    41161 tgctttagca ctttcttgta cgaatttatg aataacatta tcaggatagt ttttataacc
    41221 aactgcgttt gaagcacgta acaacatttg gaataataca tttggaatag ctttacgtag
    41281 acgttcaagt cgttcccatg ggttttcctt caagaaatta tatgccacat caaatgtagc
    41341 accgccccac atttctagtg agaaaccatc tttaaatacg tccgctgttt tggatgcgat
    41401 attaatcata tccttagttc taactcgtgt agctaataat gattggtgtg catctctaaa
    41461 ggttgtatct gttagtaaga catcatcctg ctttttaacc cattcagcta caccttttgg
    41521 acctacttca tcaagcaatt gtttcgtacc actaaatgaa gcgattttac ttgaagatac
    41581 agttggaatt gatgctaatt catagtctgg tttcggacgt ttctcaacat ttgggaaacc
    41641 attaattgtt acattaccta tatattctaa tgttttagta cctctatcta gagacggctg
    41701 aatgtcgaaa agttctggtg tttcttcaat aaatttagtt gtgtaatcac cacttgtgaa
    41761 ctttttattc ttcattacat taattaagaa tggaatatta gttttaacac cacgaatacg
    41821 catttctcgt aatgaacgta ccattttttc ttctgcttgt ttaaatgata tcgcgtgtgt
    41881 agataatttt acgagtaatg aatcataata aggtgatatc tcagcacctt ggaaaccatc
    41941 tccagcatca agacgtacac caaagccacc gcttgaacga taagcaatga ttgttccagt
    42001 atccggcatg aaatcattta acggatcttc tgttgtaata cgacattgga tggcatagcc
    42061 caatgttgta atatcttttt gttgcggcat attaatctct tcaccaaata aatcggcacc
    42121 tgctgcaact aaaatttgtg tcttaacaat atcaattcct gttaccatct ctgtaattgt
    42181 atgctctact tgtacacgag ggttaacttc tataaagaag aattcgtcac cagatactag
    42241 aaactcaaca gtacctgcat tgacatattt aatattttcc atcaattgaa ttgcagcatc
    42301 acaaatacgt tgacgtaatg ttggtgataa tccaactgat ggtgcaactt ctacaacttt
    42361 ttgatgacga cgttgtactg aacaatcacg ttcaaataag tgtacgatat ttccatgttc
    42421 gtcacctatg acttgtactt caatatgctt tggattatca atgtatcttt ctatgtaaac
    42481 ttcactatta ccaaatgatt tttctgcttc tgattttgct ctatggaaag catcttctaa
    42541 ttcactttct tcacgaacga ttctcatacc tttaccgccg ccaccacttg tggctttaat
    42601 cattagcggg aaaccagctt cttctgcaaa ttcttttgct aattcatatg atttaattgg
    42661 accgtctgta ccaggaataa ctggtaaatc tgccttgata gccgttgtac gagctttaac
    42721 tttatctcca aacatatcta aatgttctaa atgaggacca ataaatttaa tcccttcttc
    42781 tgcacaacga cgcgcaaatt gttcattttc acttaaaaat ccatagccag gatgaatcgc
    42841 atccacattc gcttgttttg ctacatcaat gatacgctca atatttaaat aactttcagc
    42901 aggacctaaa tcacttccaa ctaaatagga ttcatctgct ttatatctat gtaatgaact
    42961 tttgtcttca ttcgaataaa ttgcaactgt gctgatgtct aattctgctg ccgctctgaa
    43021 tatacgaatt gcaatttctc cacggttagc aacaagtaac ttttttattt gtttcaatag
    43081 cgatacactc ctcaaactat tagaattttc taactaatta gataataaaa ttttatctta
    43141 aagcgctctg ttttgctata gttatgtttc aaattttcaa aatttaacat tcttgagaca
    43201 attaaaacct ccgcttcaaa aataataatt tcaaaaatga ttatgcaaca acaggtagtt
    43261 cacgtttttg ttgtgaaaca ttttcgattt ctacaactct aaaaaattaa aaataaaatt
    43321 gtaaaacatc aacatttatt atcaatagcg ataactttat cttatcatca tgattctaat
    43381 tacgccacta catttagtga tttttagtca taaaatttag ttataattat acgttgtttt
    43441 gtttataaaa tttgataatc atgagtaatc tcgtaatatc aaaacaaaaa ggaagttaag
    43501 cgttgtttgg ttgcctaact tccgttattg aactcatcag tctcagtcca atttgacaaa
    43561 gagtatataa atagccaatg tttagcattg cttaatgaca gaagtcatct cgtactaaat
    43621 attggctagt atttttttaa ttaaattgtc ttcttatatc aactttttgt tgttgtttct
    43681 ttcgttgctg gtctactttg atttgtttac ctacaatcag aagtaatccc atagcaatac
    43741 ttaaactaat cattgatgat ccaccaaagc tgataaatgg caatggcaca ccagttaatg
    43801 gaattgttgc cgaaataccg ccaatgttta caaacgtttg gcttccaaag tatgtggcaa
    43861 tcccaacaca cacaagttta taaaaatatg atgatgtttt atttgcaaac tggaaggcac
    43921 gatatacaat aaagaactct aaagtaataa ctagcaatcc tccgattaaa cctaattctt
    43981 cgcaaataat tgcaaatata aaatctgtat gtggttctgg taaatagccc aatttcattg
    44041 cactatttcc taatcctttt ccaaatacgc caccgttacc tatcgcaagc aatgaattgg
    44101 aaatatggta tccagttcct gattcgaatt ggaatggatc tgttagcgta ctaaatctgg
    44161 cagttaaata acttggtaac caaccagcca ttaatgcaat gacaaatact actaagaatc
    44221 ctagcactgc tggtatacca aatcttagga ctttgtttac cccaattcct gaataaaaaa
    44281 taatagcaac taaaataatt aatattagta atgtttgccc tacgtccttt tgtaagaaaa
    44341 ctaaaaacgt acaacctaat gctaatacaa taggacttaa aattaatttt ggtttactta
    44401 atactctagg cattttttta ctgatcataa atgggatata taatatgatt gcaattttta
    44461 ataactcaga tgcctgtaag ttcataaacc ctaagtttat ccaactttta gaaccattaa
    44521 tatctttacc gattactaac gtcagtaata atagcgagac gatagttata atcatacctt
    44581 tttgcacttt aatattactc agtaatttaa cattcattaa aaatgcaata aaaaatacaa
    44641 ttataaaact cattatgaca tatgctaatt gtcggttgta aaaatacgtt cctggaacat
    44701 cgataccacc agtcaatgta cctttagttg ctggaaccat actggcacta tataccatca
    44761 ctaatccaat taaactcaat acaatatatg taactaataa cggataatca ataaactttg
    44821 aggttttacc aatataccgt aaaatacttc taaaattctt catatattca gtcatccaat
    44881 tctacaattt atacttcaaa tgatttcgtt ttatacttta attttaaaaa tccatgttac
    44941 ttattatata catgacatcg tcataatcac aaatattaac aggtatcttg aggtaattta
    45001 tagaatattt gtttacttaa tatcgtgaat tgctaaaacc caataaaagt gactgacatg
    45061 tcatcaagaa gacaacataa cagccacttt aatcagtaat taaactataa atataaaatc
    45121 ggcgcagctc aattgtcgat atctcacgcc gttttaatat gcttatatat taaaccttaa
    45181 acaagtgtaa aagcttcatg taattttgaa agttctttct caagacgtaa cataatttgt
    45241 ttgccatctt cgcggtcaac taaacctaat ttaacagcaa aatcaacttc tttttgaagt
    45301 ccaaacattt gtgtatctag tacctcttca tataatgggc atgaaggtaa tgttaaatta
    45361 tctagttgaa ctttaatcag atgcaagatt tcatcagcat ctttagtcaa ttgtttcaaa
    45421 gctgcatttt tcattgttgt ttgtttcgcc atgacacgct ccccctctat ataaattctc
    45481 aagtcttact aaaagtttat cttactaaat attaaaaagc aagtagattt aaaatgtgtc
    45541 gttagcaatt tgtgttaaaa taatgctata tacattgata gggagtggga aaatgataca
    45601 aattaaagga gccgtaaagt ttcctataac attagatagt acgacttgga ttttcgatga
    45661 tagaaaagtt agcatcgaag atttagaaaa aggtgtattt gatggttcaa aaccaatcga
    45721 ctttgaagat aatcatgaat ggaatcgtgc tatcctagaa ggacaaacta atccgcctac
    45781 tttaaattca gaaataaaat acaaaaaacg tgccatgtta gaaggttcat ttgtcattaa
    45841 tatgacacca tttttcaaac attccgaacc acatgactct gcaactacta ttcgattatc
    45901 aaatgagcat gatcatttag atataccgat agatctatta ccctatttat ttttccaatt
    45961 tgcaaaagac ggtaaacgac tttacagcga taatggtgta gatagtttta tttacaatca
    46021 agaaaatggt tattcaaatc gaatcaacca tgtaactcat attgaggtga tttaatttga
    46081 gacaagtgca atgcattatc tgtgatgcaa aagtatttat agatgagcgc acaactgagt
    46141 ctaaacgact taaaaacaat cctattcgaa catttatgtg cgatgattgt aaaagtcgat
    46201 tagacacccc taaacaacgt gcgcaacact atccgctcga ttaatatctg aataaacaag
    46261 ttagaaattt gaaaatgtga aagtaacagg aactgatacc atttaaacac tttttcaaat
    46321 attatatgcc aaaagagaag ctaagcaact ttcatcgctc agcttctctt ttattatgtt
    46381 aaattattcg ttttcttgca tcatttgttt gatacgcttt gcttcttttt cacgaacatt
    46441 tttgtttaaa attttctttc ttaaacgtat gctttctggt gtaacctcaa caagttcatc
    46501 atcattaatg aattgtaatg cttcttccaa tgttagaata cgcggtctat tcattgtttg
    46561 tgtttggtct ttcgttgcag aacgtacgtt agtttgatgt tttgttttag tgatgttaac
    46621 agttaaatca ttttcacgat tatgttcacc aacaatcata ccttcataaa cttcagtacc
    46681 aggttccatg aagtttacac ctctatcttc aagtcctaaa atggcataag tacttgcaga
    46741 accttgatcc attgaaatta atgcaccatt acgacggccg ccaatttgtg ctttaatacg
    46801 tggtctaaat tcttcaaatg tatggttaat aataccgtaa cctcttgtca ttgacataaa
    46861 ttcagtcgta taaccaatca taccacgagc cggtacatta aagattaaac gtgtaagtcc
    46921 attatcagtt gtagtcatat caaccatttc acctttacgt gcacctaatg attcaataac
    46981 cgcacctgca ttttcttgtg gcacttcaca ttgcacacgt tcaaatggtt cacacattac
    47041 accatcaatt tctttaataa ttacttgtgg ttttgaaact tgtaattcat aaccttcacg
    47101 acgcatattt tcaataagga ttgataaatg taattcacct cgaccagcaa ctacccatgt
    47161 atctggagaa tctgtgttag atactttcaa agatacatct gtttctaatt gttgatttaa
    47221 acgttcttga atttgacgcg ctgttacaaa gtcaccttca cggccagcaa atggtgaatt
    47281 gttaacttta aatgtcattt caagagtagg ctcatcaata cgtagtactg gcaatgcttc
    47341 ttgatggtca tgtggtgtta cagtttcacc aacgttaatg tcttccatac ctgaaacagc
    47401 aattaaatct ccagcctgcg cttcttcaat ttctaaacgt tttaatccaa agtaaccaaa
    47461 gattttagtt acacggaagt ttttcactgt accgtctaat ttaattagtg atacattatc
    47521 tccgacacgc attttacctc tgaatacacg accaatacca atacgtccaa cataatcatt
    47581 gtagtccaac aatgctactt ggaattgtaa tggctcatca ctgttatcaa ttggagctgg
    47641 tacataatca ataattgttt catataatga ttgtaaatta tcatcttgtt tatcaggatc
    47701 taagctagct gtaccattta ctgctgaagc ataaacaaca gggaattcta attgttcatc
    47761 gtttgcttct aattcaataa ataaatctaa aacttcatct acaacaccct ctggacgtgc
    47821 tgatggttta tcaattttat taacaacaac aacaggtttc aggttttgtt ctagcgcttt
    47881 tttaagtaca aaacgtgttt gaggcattgt accttcatac gcatctacta ctaagacaac
    47941 cccatcaacc attttcataa tacgttctac ttctccacca aagtctgcat gtcctggtgt
    48001 atccaaaata ttgatacgtg tacctttata atcaacagcc gtatttttgg ctagaatcgt
    48061 aattccacgc tctctttcga tatcgttaga gtccattgca cgttcatcga catgttcatt
    48121 ttctctgaat ataccagatt gttttaacaa ctcatctact aaagttgttt taccatggtc
    48181 aacgtgagca ataattgcta tattgcggac atcttctctt ttattagtca tataaatagt
    48241 ttcc
//
LOCUS       DAHHRR010000006        47618 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.006, whole genome shotgun sequence.
ACCESSION   DAHHRR010000006 DAHHRR010000000
VERSION     DAHHRR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 47618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47618
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.006"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            153..1271
                     /gene="ald"
                     /locus_tag="NBI42_000278"
     CDS             153..1271
                     /gene="ald"
                     /locus_tag="NBI42_000278"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCD1776744.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVLVEKNAGIGSG
                     FSNEMYEQEGAKIVSHEQAWEADLVIKVKEPHESEYQYFKKDQIIWGFLHLASSKQIV
                     EKMQEVGVTAISGETIVKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVNIPGSTYVIFGGGVAATNATNVALGLNAKVIILELNDDRIKYLKEKYTDKDVTV
                     VKSTPENLAEEIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGINIYKGQVTNKGLATSHDLDFKEILNVIK"
     gene            1365..2405
                     /gene="tdcB"
                     /locus_tag="NBI42_000279"
     CDS             1365..2405
                     /gene="tdcB"
                     /locus_tag="NBI42_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCD1776745.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQN
                     ITKGNVYLKLENMQFTGSFKFRGASNKINHLSDEQKAKGIIGASAGNHAQGVALTAKL
                     LGIDATIVMPETAPIAKQNATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQAEN
                     VHGMAESFYKRDLTEHREDSTIADGCDVKVPGEKTYEVVKHLVDEFILVSEEEIEHAM
                     QDLMQRAKIITEGAGALPTAAILSGKIDKKWLEGKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2436..3758
                     /locus_tag="NBI42_000280"
     CDS             2436..3758
                     /locus_tag="NBI42_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1776746.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALAIVFATQLINLFHLSAGLLIPVAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGNITFSLIPSTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKKPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     EYGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLLIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAMIGILITALGIPVYYYKKKQKAT"
     gene            3914..5305
                     /gene="norB"
                     /locus_tag="NBI42_000281"
     CDS             3914..5305
                     /gene="norB"
                     /locus_tag="NBI42_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HCD1776747.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGTFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGEFLISLTFLPEIIYVICCIVGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            5703..37580
                     /gene="ebh"
                     /locus_tag="NBI42_000282"
     CDS             5703..37580
                     /gene="ebh"
                     /locus_tag="NBI42_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HCD1776748.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASEFKQKQQSSNEQTENRESQVQSSQNSQNSQTLSTPNENEQSNNNQTSHVGENEAQ
                     PSTTEHEQPVSQNKQSKNDANDTVAKPQSDKKETSQEQNENHVANKPVNDNEATYVES
                     HQANVVTASDATENGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKI
                     NTLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSSRIQTRSVESRSAEPR
                     SVSDYQNANSSYYVENANDGSGYPVGTYINASNKGAPYNLPTTPWNTLKASAAKEIAL
                     ITAKQTGDGYQWVIKFNKGHAPHDNMIYWFALPADQVPVGRTDFVTVNADGTNVQWSN
                     GAGAGANKPLPQMWPNGVNDSRSWDFKIRNRSGQVIYDWPTVHINSLTDLARAGDYFS
                     EAGATAATKAFGRQIFEYINGDRPTESPGVPRVYTFIGKGDASYTISFKTQGPTINKL
                     YYAAGARALEYNQLFMYSQLYVESTQDQQQRLNGLRQTVNRTYRLGTTKRVEVSHGNV
                     QTKKVLESSNLNIDDFVDDPLSYVKTPSNKVLGFYPTNANANGFRPGGVQELNEYQLS
                     QLFTDQKLQDAARTRNPIRLMIGFDYPDGYGNSETLVPVNLTVLPEIQHNIKFYKNDD
                     TQNIADKPASRQAGHPIFYVYAGNQGNASVNLGGSVTTIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRTNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVTVSNIPLDPSALVYLITPTAQTTNGGSEADQIPSNYTIL
                     ATGTPDGVHNTITIPPKDYVVFIPPEGRQIKAMIYYNKFVASNFSNGVTILPDNIPPT
                     LENPVGINAKYYRGDEVSFTIAAADRHTGLKNTTITTLPSGWTSNLAKTNSDHNGTIT
                     ITGRVAMNQAFNSDITFKVAATDNANNTTNDNQSKHVSIHVGQISEDAHPIVLGNSEK
                     VVVVNPTALSNNEKQSIINAFMNKNQNIRGYLASSNPVVVDNNGNVTIHYRDGSSTTL
                     DATNVMTYEPVVKSEYQTANAPKTATVTIAKGQSFSIGDIKQYFTLSNGQAIPSGTFT
                     NITSDRTIPTAQEVSQMNAGTQLYHITASNAYHKDTEDFYITLKIVDVKQPEGDQRVY
                     RTSTYDLTADEISKVKQAFINANRDAITFAEGDISVTNTPNGANVSTITVNINKGRLT
                     KSFTSNLANMNFLRWVNFPQDYTVSWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLG
                     TQITTNDILTMLKATTTVPGLRNNITGNEKAQAETGGRPNYRTTGYSQSNATTDGQRQ
                     FTLNGQVIQVLDIINPSNGYGGQSVANSNVRANHSNSTVVNVNEPAANGAGAFTIDHV
                     VKNNSTRNATDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTIS
                     VGNYANHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTS
                     ATTKTINLVATDTSGNTATTSFNVTVKPLRDKYRVGTSSTTANPVRIANIANNATVSQ
                     ADQTAIINSLTFTETVPNRSYVRASANEITSKTVSNVSRTGNNAHVTVTVTYQDGTTS
                     TVTVPVKHVIPEIVSNPHYTVQGQDFPAGNGSSASDYFKLSNGSAVPDATITWVSGQA
                     PNKDNTRIGEDINVTAHILIDGETTPITKTSTYKVVSSVPKHVFETNRGVVFPGVSDV
                     YDAKQYVKPVNDSWTQNAQRMNFQFTNSYGPSKDVVGISTRDIRVTYDNHQTQIIKIL
                     AKVKPDPPRIDGNSVTYKAGLTNQQIKINNVLSSSSIKLFKADNTPLTITNTTYGSGN
                     TAVVTVSDALPNGEIKARSSISMNNVTYTTQDEHGRAVDVTRTESVDSNDSATVTVTP
                     QLQATSQGPVFIKGGDGFDFGHVERFIQNPPHGATVTWQDNPDTWKNTVGNTHKTAVV
                     TLPNGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTHGTNAIDYITFDPNTNTNGITA
                     TWANRQQPNNQQAGVQSLNVDVTYPGISAAKRVPVTVNVYQFEFPQNSYTTTVGGTLA
                     SGTQASGYAQMQNATGLPTDGFTYKWNNAATGTNDANWAAMNKPNMAKVVNAKYDVIY
                     NGHTFATSLPAKFVVKDVQPAKPTVTETTAGVITIAPGANQTVNTHAGNVTTYADKLV
                     IKRNGSVVTSFTRRNNTSPWMKEASAANVTGIVGTNNGITVAAGTFNPADTIQVVATQ
                     GSGETISDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYT
                     EKVGNGAEHSKTLSVVRGQNNQWTIANKPDYVTLDAHTGKVTFNANTIKPNSAITITP
                     KAGTGHSASSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNG
                     TAMPTNLAGGSTTTIPVTVTYNDGSSEEVQETIFTKADKRELITAKNHLDDHVSTEGK
                     KPGTITQYNNAIHNAQQQINAAKTEAQQVINNERATPQQVSDALTKVRAAQTKINEAK
                     ALLQNKEDNSQLVTSKNNLQNSVNQVPSTTGMTQQSIDNYNAKKREAESEITAAQRVI
                     DNGDATAQQISDEKQRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPAS
                     ITAYNNSMHALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNDRLTQAINQLV
                     PLADNSALRTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGD
                     ATDQQIAEEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSAS
                     VATFNEKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKSALDQARNGLTVDKAP
                     LENAKTQLQHSIDTQTSTTGMTQDSVNAYNAKLTAARNKIQQINQVLAGSPTVDQINT
                     NTSAANQAKSDLDHARQALTPDKTPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKL
                     QAARQKLAEINQVLNGNPTVQNINDKVTEANQAKDQLNTARQGLTLDRQPALNTLHGA
                     SNLNQAQQNNFTQQINAAQNHAALETIKSNITALNNAMTKLKESVADNNTIKSGQNYT
                     DATQANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAK
                     TEATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTNLKHGVANH
                     NQVLQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEQA
                     LNGNTKLNAAKQEANTALGHLNNLNNAQRQNLQSQINNAHQIDAVNTIKQNATNLNSA
                     MGNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVDDVNRA
                     TSAVTSNKNALNGDEKLAQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTV
                     KQQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNQARGA
                     NKTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTPAQKTNLTNQINS
                     GTTVAGVHMVQSNANTLDQAMNTLRQSIANKDETKASEDYLDANNDKQTAYNNAVAAA
                     ETIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLATAKQNAKTYLNTLTSITDAQ
                     KNNLISQITSATRVSGVDTVKQNAQHLDQAMSNLQNGINNESQVKSSEKYRDADTNKQ
                     QEYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNTAKDALNGDAKLIAAQNAAKQHL
                     GTLTHITTAQRNDLTNQISRATNLAGVESVKQSANSLDGAMGNLQTAINDKAATLASQ
                     NFLDADEQKRNAYNQAVSAAETILNKQNGPNTAKSAVEQALNNLNTAKHALNGTQNLN
                     NAKQAAITAINGASDLNQKQKDALKAQANGAQRVSNAQDVQRNATELNTAMGTLKHAI
                     ADKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAK
                     QELNGDERLRVAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQSVQTNGQALN
                     NAMKGLRDSIANETTVRASQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMEASAIT
                     QATTQVNNGKNGLNGAENLRNAQNAAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVT
                     AAKNAATELNTQMGNLEQAIHDQNTVKQSVKFTDADKAKRDAYTNAVSRAEAILNKTQ
                     GANTSKQDVEAAIQNVSSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKI
                     DQATTVAGVEAVSNTGTQLNTAMASLQNGINDKANTLASENYHDADSDKKTAYIQAVT
                     NAENILNKNSGSNLDKAAVENALSQVTNAKGALNGNHNLEQAKSNANTTINGLQHLTT
                     AQKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNAATKNGQNYLDATES
                     NKTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATN
                     AIDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQ
                     TQKYRDAEQSKKTAYDQAVAAAKAILNKQSGSNSDKAAVDRALQQVTSTKDALNGDAK
                     LAEAKAAAKQNLGTLNHITNAQRTALEGQINQATTVDGVNTVKTNANTLDGAMNSLQG
                     SINDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTR
                     AKAALNGAENLRNAKTSATNTINGLPNLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNT
                     LNTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANA
                     INGMANQVNTTKAALNGAQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSD
                     ANNVQHTATELNSAMTALKAANADKERTKASGNYVNADQEKRQAYDSKVTNAENIISG
                     TPNATLTANDVNSATSQVNAAKTALNGDNNLRVAKENANNTIDGLSQLNNAQKAKLKE
                     QVQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSA
                     VTAAKAIINQTSNPTMEPNTITQATSQVTTKEQALNGAQNLAQAKTTAKNNLNNLTSI
                     NNAQKDALTHSIDGATTVAGVNQESAKATELNNAMHSLQNGINDETQTKQTQKYLDAE
                     PSKKSAYDQAVTAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAA
                     KQTLGTLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTT
                     LQSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGI
                     QNLERAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTAL
                     KRAIADKAETKASGNYVNADANKRQAYDEKVTNAENIVSGTPTPTLTPSDVTNAATQV
                     TNAKTQLNGNHNLEVAKQNANTAIEGLSSLNGPQKAKLKEQVGQATTLPNVQTVRDNA
                     QTLNTAMKGLRDSIANEATIKAGQNYTDASQNKQTDYNNAVTAAKAIIGQTSNPTMNA
                     QEINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTDAQKDAAKRQIEGATHV
                     NEVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQIL
                     NKAQGPNTAKDNVESALQNVQRAKNELNGNQNVENAKTTAKNALNNLTSINNAQKEAL
                     KSQIDSATTVAGVNQVSTTASELNTAMSNLQNGINDEAATKAAQKYTDADRDKQTAYN
                     DAVTAAKTLLDKTAGSNDNKAAVEQALQRVNTAKSALNGDARLNEAKNTAKQQLATMS
                     HLTDAQKANLTEQIERGTTVAGVQGIQANAGTLDQAMNQLRQSIASKDATKSSEDYQD
                     ANADLQNAYNRAVSDAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQ
                     TAKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMSNLKNALAEKD
                     NTKRSVNYTDADQPKQQAYDTAVTQAEGITNANGSNANETQVQAVLNQLNQAKSDLNG
                     NNKVAQAKEAAKRALASYSNLNNAQSTAATSQIDNATTVAGVTAAQNTANELNTAMGQ
                     LQNGINDQNTVKQQVNFTDADQGKKEAYTNAVTNAQGILDKANGQNMTKAQVEAALNQ
                     VTTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIESATTVNGVNGVKTK
                     AQDLDGAMQRLQSAIANKDQTKASENYIDADPTKKTAYDNAITQAESYLNKDRGANKD
                     KQAVEQAIQSVTTTENALNGDANLQRAKSDATQAIDNLTHLNTPQKTALKQQVNAAQR
                     VSGVTDLKNSATSLNNAMDQLKQSIADHDTIVAGGNYTNASPDKQGAYTDAYNAAKNI
                     VNGSPNVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQS
                     ITGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDEVKASQPYIDADTDKQNAY
                     NTAVTSAENIINATSQPTLDPSAVTQAANQVNTNKTALNGAQNLANKKQETTANINRL
                     SHLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFT
                     DADPEKQQAYTSAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAK
                     NNANQTLSTLDNLNNAQKAAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDK
                     DTTLSSQNFTDADPEKKNAYNEAVRNAENILNKSTGTNVPKDQVEAAMNQVNTTKAAL
                     NGTQNLEKAKQHANTAIDGLSHLTNAQKDALKQLVQQSTTVAEAQGNEQKANNVDAAM
                     DKLRQSIADNATTKQNQNYTDASPNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAA
                     GQVSTTKNALNGNENLEAAKQQATQSLGSLDNLNNAQKQAVTDQINGAHTVDEANQIK
                     QNAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGN
                     ATQAEVEQAIQQVNATKQALNGNANVQHAKDEATALINNSNDLNQAQKDALKQQVQNA
                     TTVAGVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYKQAVDKAE
                     ALISGTPDAVVTPSEITAALNKVTQAKNGLNGNTNLATAKQNVQHAIDQLPNLNQAQR
                     DEYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIADKAQIKGSENYHDADTDKQT
                     AYDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTFG
                     TLDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLSNALQDKTETLNSIN
                     YTDADQGKKDAYTNAVAHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQR
                     AKDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIS
                     DKDQTKANGNFVNADPEKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVTQAKD
                     DLNGNHNLQVAKDNANTAIDNLPNLNQPQKSALKDQVSHAELVTGVNAIKQNADALNN
                     AMGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGTPTPVLTPDTVTQ
                     AVTTMNQAKDALNGDEKLAHAKQEALANLDTLRDLNQPQRDALRNQINQAQALATVEQ
                     TKQNAQNVNTAMGNLKQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTN
                     PTLNPDEITRALTQVTDAKNGLNGDAKLATEKQNAKDAVNAMTHLNDAQKQALNGQID
                     QSPEIATVTQVKQTATSLDHAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAK
                     AESLLDKQSGTNEVQAQVERITNEVNTAKQALNGNDNLANAKQQAKQQLANLTHLNDA
                     QKQSFESQITQAPLVTDVTTINQKAQALDHAMELLRNSIADNQATLASEDYHDATAQR
                     QNDYNQAVTAANNIINQTTSPTMNPDEVNSATTQVNNTKVALDGDENLAAAKQQANNR
                     LDQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQR
                     GNYVNADTDKQTAYTTAVNEAEAMINKQTGQNANQTEVEQAINKVQTTLQALNGDHNL
                     QVAKANATQAIDALSSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRES
                     IQDNAATKANSIYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTK
                     AALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVNHDLTEAQALD
                     QLMDALQQSIADKDATHASSAYVNAEPNKKQAYDEAVQNAESIIAGLNNPTINKGNVS
                     SATQAVTSSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRDKVA
                     EIIAQAQALNEAMKALKESIKDQPQTEASSKFINEDQAQKDAYTQAVQHAKDLINKTT
                     DPTLAKSIIDQATQAVTDAKNNLHGDQKLAQDKQRATETLNNLSNLNTPQRQALENQI
                     NNAATRGEVAQKLTEAQALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQ
                     NAKDLINQTSNPTLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNN
                     PQRQALESQINNAATRGEVAQKLAEAKALDQAMQSLRNSIQDQQQTESGSKFINEDKP
                     QKDAYQAAVQNAKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVT
                     TVIALPNLNHAQQQALTDAINAAPTRTEVAQHVQTATELDHAMEALKNKVDQVNTDKA
                     QPNYTEASTDKKEAVDQALQAAQSITDPTNGSNANKDAVEQALTKLQEKENELNGNER
                     VAEAKTQAKQNIDQLTHLNADQIATAKQNIDQATQLQPIAELVDQATQLNQSMGQLQQ
                     AVNDHTNVEQTVDYTQADSDKQKAYKQAIADAENVLKQNANKQQVDQALQNILNAKQA
                     LNGDERVELAKSNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNHA
                     MDQLSQEITGNEGRTKGSTNYVNADTQVKQVYDEAVDKAKQALDKSTGQNLTAEQVIK
                     LNDAVTAAKEALNGEERLNNRKSEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASR
                     AINRAVQLDDAMGAVQQYIDEHHLDVISSTNYINADDNLKANYDNAIANAAHELDKVQ
                     GSAIAKAEAEQLKQNIIDAQNALNGDQNLANAKEKANAFVDTLNGLNQQQQDHAHQAI
                     NNADTVVGITDIVNNQIDLNDAMETLKQLVDNEIPTAEQSVNYQNADDNAKSNFDDAK
                     RLANTLLNSDNTNVNDINGAIQSVNDAIQNLNGDQRLQEAKDEAIQSINQALADKLKE
                     IEASNATDQDKLIAKNKAEELANSIINNINKATSNQAVTEVQTAGNQAIEQVHANEIP
                     KAKIDANNDVDNRVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNDINNAMTKEE
                     IEQAKERLAQALQDIKDLVKAKENAKQDVDNRVQALIDEIDRNPNLTDKEKQALKDRI
                     NQILQQGHNDINNAMTKEEIEQAKERLAQALQDIKDLVKAKEDAKQDVDKQVQALIDE
                     IDQNPNLTDKEKQALKDRINQILQQGHNDINNALTKEEIEQAKAQLAQALQDIKDLVK
                     AKEDAKNAIKALANAKRDQINSNPDLTPEQKAKALKEIDEAEKRALQNVENAQSKDQL
                     NQRLNLGLEDIRNTHVWEVDEQPAVNDISEATPEQILVNGELIVHRDDIITEQDILAH
                     INLIDQLKAEVIDTPSTATISDSLTAKVEVTLLDGSKVIVNVPVKVVEKELTVVKQQA
                     IESIENAAQQKINEINNHATLTPEQKEAAIAEVNKLKQQAIEQINNAADVHTVEEVQN
                     QEQAHIEQFNPDQFTIDQAKSNAIKSITDAIQHMIDEINASKDLTDKEKQEAISKLNQ
                     LKDQSIQAIQRAQSIVEITQQLDQFKAQLKAANPFAKELENRKKAAISKIKDISTDKI
                     DRIRNSTIGTAEERQAAMNRINEIVLETIRDINNAHTLQQVEAALNNGIERILAVQIV
                     TSDHSKHSSNSDGQSNSHLHVGYGTVNHPFNSSPIGHKKKLDQDDEIDPLHMRHFGDR
                     IGNVIKNALGVVGISGLLASFWFFIAKRRRKEDEEEELEIRDNSKDKKKGSIEGTKHL
                     PLLFAKRRRKEDEEDAIVEEKDSLNNDESLDKVKHTPFFLPKRRRKEDEEDVEVTNEN
                     TDEKVLQDNEHSPVLIAKRRRDKEEDVETTTSIESKDEDVPLLLAKKKDQKDNQSKGK
                     KSASKKTSKKVAAKKKKKKSKKNKK"
     gene            complement(37639..38040)
                     /locus_tag="NBI42_000283"
     CDS             complement(37639..38040)
                     /locus_tag="NBI42_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCD1776749.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALKHARELNVSNALLYTDSKLIADSVNAGYVKNAKFKPYFDQLEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            complement(38276..38980)
                     /locus_tag="NBI42_000284"
     CDS             complement(38276..38980)
                     /locus_tag="NBI42_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCD1776750.1"
                     /translation="MYNEILGLVTFIVTFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            39225..39419
                     /locus_tag="NBI42_000285"
     CDS             39225..39419
                     /locus_tag="NBI42_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCD1776751.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            39431..39682
                     /locus_tag="NBI42_000286"
     CDS             39431..39682
                     /locus_tag="NBI42_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCD1776752.1"
                     /translation="MQIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            39720..40844
                     /locus_tag="NBI42_000287"
     CDS             39720..40844
                     /locus_tag="NBI42_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HCD1776753.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPEFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANQVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYLEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            40860..41297
                     /locus_tag="NBI42_000288"
     CDS             40860..41297
                     /locus_tag="NBI42_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCD1776754.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVLEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            41717..42673
                     /locus_tag="NBI42_000289"
     CDS             41717..42673
                     /locus_tag="NBI42_000289"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCD1776755.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDNTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            42862..43341
                     /locus_tag="NBI42_000290"
     CDS             42862..43341
                     /locus_tag="NBI42_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD1776756.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            43356..44195
                     /gene="fakB2"
                     /locus_tag="NBI42_000291"
     CDS             43356..44195
                     /gene="fakB2"
                     /locus_tag="NBI42_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCD1776757.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGTSYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGAEIISIHLSSGLSGTFNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSVGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            44281..44814
                     /gene="msrA"
                     /locus_tag="NBI42_000292"
     CDS             44281..44814
                     /gene="msrA"
                     /locus_tag="NBI42_000292"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD1776758.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            44807..45235
                     /gene="msrB"
                     /locus_tag="NBI42_000293"
     CDS             44807..45235
                     /gene="msrB"
                     /locus_tag="NBI42_000293"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCD1776759.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            45247..45747
                     /locus_tag="NBI42_000294"
     CDS             45247..45747
                     /locus_tag="NBI42_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1776760.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            45747..45968
                     /locus_tag="NBI42_000295"
     CDS             45747..45968
                     /locus_tag="NBI42_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCD1776761.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            46150..>47618
                     /locus_tag="NBI42_000296"
     CDS             46150..>47618
                     /locus_tag="NBI42_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCD1776762.1"
                     /translation="MDDKQHTSSSDDERAGIATSNQDQQTNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQSSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNETTQFDQALENQV
                     KAFQQTNKLEVTGEFNKETNNKFTELLVEKANKHDDVLD"
BASE COUNT        18753 a         7987 c         8542 g        12336 t
ORIGIN      
        1 acattatatt tataaacaag aattttatat taaatattta ttaacaaatt atagataaaa
       61 tatgaataat taattaatgg tatttacata tgcatgtcca atagataaaa tgattgtgaa
      121 ttaattcaca aatataaaga ggagtgttgc aaatgttagt tgcagtagtc aaagaattaa
      181 aacaaggcga aggtcgtgta gcttgcacac ctgaaaatgt gcgtaagtta acagatgcag
      241 gtcataaagt actcgttgaa aaaaatgcag gtatcggttc aggcttttcc aacgaaatgt
      301 atgagcaaga aggcgcaaaa attgtaagtc acgaacaagc atgggaagca gacttagtta
      361 tcaaagttaa agaacctcat gaaagtgaat accaatattt taaaaaagat caaattatct
      421 ggggattcct acatttagct tcttctaaac aaatagttga aaaaatgcaa gaagttggtg
      481 taactgctat tagtggtgaa acaattgtga aaaatggaaa agcggaatta cttgcaccaa
      541 tgagtgctat agcaggtcaa cgttcggcga ttatgggagc gtattactct gaagcgcaac
      601 atggtggaca aggtacatta gtgactggtg ttcatgaaaa tgtgaatatt ccaggaagta
      661 catatgtcat atttggtggc ggagttgcag caacaaatgc tactaatgtt gcattaggat
      721 taaatgccaa agtaattatt ctagagttaa atgatgaccg tattaaatat ttaaaagaaa
      781 aatatacaga taaagatgtc acagtagtca aatcaacacc agaaaattta gcagaggaaa
      841 ttaaaaaggc agatgtattt atttctacaa ttttaattcc tggagctaaa ccaccaaaat
      901 tggttactcg tgaaatggtt aaatcaatga aaaaaggttc agtcttaatc gacatagcta
      961 ttgaccaagg tggaaccata gaaacaatca gaccaactac aatttctgat ccagtgtatg
     1021 aagaagaagg tgtaatccat tacggtgtac ctaatcaacc aggtgctgta ccaagaactt
     1081 caacaatggc gttagcgcaa ggaaatattg attatatttt agaaatttgt gacaaaggat
     1141 tggaacaagc cattaaagat aacgaagcat taagcactgg aataaatata tataaaggtc
     1201 aagtgacaaa caaaggatta gcaacatctc atgatcttga ttttaaagaa atcttaaatg
     1261 tcattaaata aaaagtctgt aattgaaaaa gagtgaaatg aatttttata taaataagca
     1321 atatttggac taaaaattta caaaacggaa ggatgtaatc aattatgaca accaacacag
     1381 taacattaca aacagcacac attgtaagtt taggagatat cgaagaagca aaagcaagca
     1441 ttaaaccatt cattcgcaga acgcctctta ttaaatctat gtatttaagt caaaatatca
     1501 ctaaaggtaa cgtatattta aaacttgaaa atatgcagtt tacaggttca tttaaattta
     1561 gaggtgctag taataaaatt aatcatttgt cagatgaaca aaaagctaaa ggtattattg
     1621 gcgcatcagc cggaaatcat gcacaaggtg tagctttgac agcaaaatta ttaggtattg
     1681 atgcaacgat tgtgatgcca gaaacagcac caattgctaa acagaatgct accaaaggat
     1741 atggtgcaaa agtcatttta aaaggtaaaa actttaatga aactagactt tatatggaag
     1801 aattagcgaa agaaaatggt atgacaattg ttcatccata tgatgataag tttgtcatgg
     1861 ctggtcaagg aacaataggc ttagaaattt tagatgatat ttggaatgtt aatacggtca
     1921 ttgttccagt tggtggaggt ggcctaattg caggtattgc caccgcttta aaatcattta
     1981 acccatctat tcatattatc ggcgttcaag ctgaaaatgt gcatggcatg gcagaatcat
     2041 tctataagag agacttaact gaacatcgtg aagatagcac aattgcagat ggttgtgatg
     2101 taaaggtacc aggtgaaaaa acttacgaag tagtaaaaca tttagtagat gaatttattc
     2161 ttgtatcaga agaggaaata gaacatgcaa tgcaagattt aatgcaacgt gccaaaatca
     2221 ttacagaagg tgcaggagca ttacctacag cagctatttt aagtgggaaa attgataaaa
     2281 aatggctaga gggtaaaaat gtcgttgcat tagtttcagg aggaaacgtt gacttaacaa
     2341 gagtttctgg tgtcattgaa catggattaa atatcgcgga tacaagtaag ggtgtggtag
     2401 gttaaaactt ccaatctaaa aaatgaggtg taattatgtc aaatggtaaa gaattacaaa
     2461 aaaatatagg tttcttctca gcgtttgcta ttgttatggg gacagttatt ggttcaggag
     2521 tattctttaa aatatcaaac gtaacagaag taacaggaac agcaggaatg gccttgtttg
     2581 tatggttcct aggcggcatc attaccattt gtgcggggtt aacagcagca gaacttgctg
     2641 ctgcaatccc tgaaacaggt ggcttaacga agtatataga atatacatat ggtgatttct
     2701 ggggattcct ttcaggatgg gctcaatcat ttatatattt tccagcaaac gtcgcagccc
     2761 tcgctatcgt ttttgcgaca caattaatta atttattcca tttatcagca ggattattaa
     2821 ttcctgtagc tatcgcttct gcattatcta tagtgctgat taatttcttg ggatctaaag
     2881 caggcggtat tttacaatct gtaacattag taattaaact tataccaatt atcgtcatcg
     2941 ttatatttgg tattttccaa tcagggaata tcacattttc cttaattcct tctactggta
     3001 attctggaaa tggttttttt acagcaattg gtagtggttt attagctaca atgtttgcat
     3061 atgatggttg gattcatgtt ggaaatgttg caggtgaact taaaaaacct aagcgagatt
     3121 taccattagc tatttcagta ggtatcggat gtattatggc agtatattta cttataaatg
     3181 cgacattttt attaacactt ccaattgaat tactggctgg taacttaaat gcagcttccg
     3241 atacatcaaa aatattattt ggtgaatatg gcggtaaaat tattacaatt ggtatcttaa
     3301 tatctgttta cggtacgata aatggttaca caatgactgg tatgcgagta ccttatgcta
     3361 tggctgagag aaagttatta ccatttagtc acttgtttgc aaaattaaca aaatcaggtg
     3421 caccatggtt tggagcaatt atacaattgt tgattgcaat tatcatgatg tcaatgggtg
     3481 catttgatac gattacgaac atgttaattt ttgtcatctg gttattctat tgtatgtcct
     3541 ttgttgcagt aattatttta agaaaacgag agccgaatat ggaaagaccg tataaagtac
     3601 ctctatatcc aatcatacct ttaattgcaa ttctggcagg atcatttgta ttaattaata
     3661 cattatttac gcaatttatt ctggcgatga ttgggattct aattacagcg ctaggtatac
     3721 cagtttatta ctataagaag aaacaaaaag caacataagg taagataact agcattgaga
     3781 ataaatggat ggactactaa taaatttaaa gttttacaca ttaaaatcaa aaaccattca
     3841 attattctat cgaacagaca aatttctgtt atggaatttg tctgttttca aaagtatagg
     3901 gaggcaaata gagatggaaa agccgtcaag agaggcattt gaaggcaata ataagttgtt
     3961 aataggaatt gttttaagtg taataacgtt ttggctattt gcacaatcat tggttaatgt
     4021 tgtaccaata cttgaagata gtttcaatac agatattgga acggttaata tcgccgttag
     4081 tataactgct ttattttcag gaatgtttgt agtaggagca ggtggtcttg ctgataaata
     4141 tggcagaatt aaactcacga acattggtat tatcttaaat atattgggtt cattattaat
     4201 cattatttca aatattcctt tattacttat tataggaaga ttaattcaag gactttcagc
     4261 agcatgtatt atgcctgcaa ctttgtctat tattaagtca tattacattg ggaaagatag
     4321 acaacgcgct ttaagttatt ggtcaattgg ctcatggggc ggctctggtg tttgttcatt
     4381 ttttggaggt gcagttgcaa cgcttttagg ttggcgttgg attttcatcc tatcaattat
     4441 aatttcatta attgcgctgt ttcttattaa aggcacacct gaaactaaat ctaaatcgat
     4501 ttctctaaat aaatttgaca ttaaaggtct ggttctttta gtcattatgc tcctcagttt
     4561 aaatatttta attactaaag gatcagaatt aggtgtaacc tcacttcttt ttatcacatt
     4621 attagcaatt gcaattggaa cttttagttt atttatagtt cttgaaaagc gtgctacaaa
     4681 tcctttaatc gattttaaat tatttaaaaa taaagcttat acaggtgcaa cagcttcaaa
     4741 ctttttgtta aatggtgttg caggaacatt aatagtagcc aacacatttg ttcaaagagg
     4801 tttaggatat tcttcattgc aagcaggtag tttgtcaatt acctatttag tgatggtact
     4861 tattatgatt cgtgtaggag aaaagttact tcagacactt ggttgtaaga aaccgatgtt
     4921 aattggtaca ggagtcctaa tagtcggaga gtttctgatt tcattaactt tcttaccaga
     4981 aataatatat gttatttgtt gtatcgtcgg ctatttattc tttggattag gacttggaat
     5041 ttatgcgacg ccatcaacag atactgcaat agcgaatgca ccattagaaa aagtaggtgt
     5101 tgctgcagga atttataaaa tggcttctgc attaggtgga gcatttggcg tcgcattgag
     5161 tggtgcagtt tatgcaatcg tatcaaatat gacaaacatt tatactggtg caatgattgc
     5221 attatggtta aacgcaggaa tgggaatttt atcattcgtt atcattttgt tacttgtgcc
     5281 taaacaaaac gacactcaat tatgataatt gagaattaat ttgaaatcat acaagtcgct
     5341 acaatattaa acaaaaatat aaaccgattc ttatgtatca ttattttaaa tgaacatagg
     5401 gattggtttt ttattactct tttacactac tttatttata attattataa attgtcacaa
     5461 attcaattta cctaacaata tattttgtgt tattatattc tggagcataa ataaattgtt
     5521 caacacatag ttgtaatgtg tttcaatact ttttggatag attgcgaaat tgtattgaat
     5581 cgtcatcgtt ttaaattttt aaatgagaat ggaatgagca ttacaataca caagcaatca
     5641 aaagtaaata cattcacaac acaacagaga cataacaaca agataaggag tgaacaatag
     5701 ctgtgaatta tcgtgataaa attcaaaagt ttagtattcg taaatataca gttggtacat
     5761 tttcaactgt cattgcgaca ttggtatttt taggatttaa tacatcacaa gcacatgctg
     5821 cagaaacaaa tcaaccagca agtgaattta aacagaagca acaaagtagt aatgaacaga
     5881 ctgagaatcg agaatctcaa gtacaatctt ctcaaaattc acaaaatagt caaacattat
     5941 ctacacctaa tgaaaatgaa caatcgaata acaatcaaac aagccatgta ggtgaaaatg
     6001 aagcacaacc gtctacaact gaacatgaac aacctgtatc tcaaaataag caatctaaaa
     6061 atgatgcaaa cgatactgtt gcaaaaccac aatctgataa aaaagaaacg agtcaggagc
     6121 aaaatgaaaa tcatgttgca aacaaaccag taaatgacaa cgaagcaacg tatgtagaaa
     6181 gtcatcaagc aaatgtagta actgcttcag atgcaacaga aaatggcaat gtacaacatg
     6241 atcgaaatga attacaagca ttttttgatg caaattatca tgattatcgc tttattgacc
     6301 gcgaaaatgc agattctgga acatttaact atgtaaaagg catttttgac aaaattaata
     6361 cattgctagg tagcaacgat ccaattaata ataaagactt gcaacttgca tacaaagaat
     6421 tagagcaagc agttgcttta attcgtacaa tgcctcaacg tcaacagaca agtcgacgat
     6481 caagtagaat tcaaacacgt tcagttgaat caagatcagc agaaccaagg tcagtatcag
     6541 actatcaaaa tgcaaactca tcttattatg ttgaaaatgc taatgatggt tctggttacc
     6601 ctgtaggtac gtatattaat gcttcaaata aaggggcacc atacaattta ccaactacac
     6661 catggaatac gctgaaagca tcagctgcaa aggaaatagc tctgatcaca gcgaaacaaa
     6721 ccggagacgg ttatcaatgg gttattaaat ttaataaagg acatgcacca catgataata
     6781 tgatttactg gtttgcttta ccggcagatc aagtgccggt tggacgcact gactttgtaa
     6841 ccgtgaatgc agacggtaca aatgtacaat ggagtaatgg tgcgggagca ggtgcaaata
     6901 aaccgcttcc acaaatgtgg ccaaatggtg taaatgactc tcgttcgtgg gactttaaaa
     6961 ttagaaatag aagtggacaa gtaatctatg attggccaac tgttcatatt aatagtttaa
     7021 ctgatttagc tcgtgcagga gattatttca gtgaagctgg tgcaacagct gctacgaaag
     7081 ccttcggtag acaaatcttt gaatatatta acggtgatag accaactgaa tcaccaggtg
     7141 tgcctagagt ttatactttt attggtaaag gtgatgcgag ttatacaatt tcttttaaaa
     7201 ctcaaggtcc aacaatcaat aaattgtatt atgctgcagg tgctcgtgca ttagaataca
     7261 atcaattatt tatgtacagt cagttatatg ttgaatcgac acaagaccaa caacaacgtc
     7321 ttaatggttt aagacaaact gttaatagaa catatcgctt aggtacaact aaacgagttg
     7381 aagtgagcca tggtaatgta caaactaaaa aagtacttga aagttcaaac ttaaacattg
     7441 atgacttcgt tgatgatcct ttaagttacg ttaagacacc aagtaacaaa gtgttaggat
     7501 tttatccgac aaatgcaaat gctaatggct ttagaccagg tggtgttcaa gaattaaatg
     7561 aatatcaatt aagccaatta tttaccgatc aaaagttgca agatgcagct agaacaagaa
     7621 atccaataag attaatgatt ggttttgact atcctgatgg ttatggaaac agtgaaactt
     7681 tagttcctgt taacttaact gtattacctg aaatccaaca taatattaaa ttttataaaa
     7741 atgacgatac tcaaaatatt gctgataaac cggcttcaag acaagctgga catccaattt
     7801 tttatgtata tgcaggtaac caagggaatg catctgtaaa tttaggtggc agtgtaacaa
     7861 cgattcaacc attgcgtatt aatttaacaa gtaatgaaaa ctttacagat aaagattggc
     7921 aaattactgg tattccgcgt acattacaca ttgagaattc aactaaccgt actaataatg
     7981 ccagagaacg taacattgaa cttgttggta acttattacc aggggattac tttggaacga
     8041 tacgttttgg ccgtaaagaa caattattcg aaatacgtgt taagccacat acaccaacaa
     8101 ttacaacaac agctgaacag ttgaggggaa cagcattaca aaaagtgcct gtaacagttt
     8161 ccaatattcc gttagatcct tcagcattag tttatttgat tacaccgaca gctcaaacaa
     8221 ctaatggagg tagtgaagcg gatcaaatac catctaatta tacaatactt gcgacaggta
     8281 cacctgatgg tgtgcataat acaataacga taccacctaa agattacgta gtatttatac
     8341 cacctgaagg tagacaaatt aaagcaatga tttattacaa taaatttgta gcatcgaact
     8401 ttagtaatgg tgtcacaatt ttgccggata acattccgcc aacattggaa aatccagttg
     8461 gtatcaatgc aaaatattat cgtggtgatg aagttagctt tacaattgca gcagctgata
     8521 gacatactgg attaaaaaat acgacaatta ctacattacc aagcggatgg acatccaatt
     8581 tagctaaaac taatagtgac cataatggaa caattacaat tacaggtaga gtcgctatga
     8641 atcaggcgtt taatagtgat attactttta aagttgcagc aactgataat gcaaataata
     8701 cgacgaatga taatcaatct aaacatgtgt caattcatgt aggacaaatt agtgaagatg
     8761 ctcatccaat tgtattagga aactcagaaa aagttgtagt agtaaatcca actgctctat
     8821 ctaataatga aaagcaaagc attattaatg cttttatgaa taaaaaccaa aatataagag
     8881 gttatttagc atcatcaaat ccagttgttg ttgataataa tggaaatgtc acaattcatt
     8941 atcgtgatgg ctcatcaaca acacttgatg ctacaaatgt gatgacatat gaaccagttg
     9001 ttaagtctga ataccaaact gccaatgccc ctaaaacggc aacagtaaca attgctaaag
     9061 gacaatcatt tagtattggt gatattaaac aatatttcac tttaagcaat ggacaggcta
     9121 ttccgagtgg aacatttaca aatattacat ctgatagaac gattccaaca gctcaagaag
     9181 taagtcaaat gaacgcaggc acacagttat accatataac tgcttcaaat gcgtatcata
     9241 aagacactga agatttctat atcacattga aaatagttga tgtgaaacaa cctgaaggcg
     9301 atcaacgtgt ctatcgtact tcaacatatg acttaacagc tgatgaaatt tcaaaagtta
     9361 aacaagcatt tattaatgcg aatagagatg caattacgtt tgctgaaggt gatatttctg
     9421 taactaatac accaaatgga gccaatgtaa gtacaatcac agtcaatatt aataaaggtc
     9481 gcttaacgaa atcattcacg tctaatcttg caaatatgaa tttcttgcgt tgggttaatt
     9541 tcccacaaga ttacacagta tcttggacaa atgcgaaaat tgcaaatcgt ccaactgatg
     9601 gtggtctatc ttggtctgat gatcataaat cattaattta tcgttatgat gcgactttag
     9661 gtactcaaat tacgacgaat gacattttaa caatgttaaa agcaacaact acagtgcctg
     9721 gattgcgtaa taacattact ggtaatgaaa aagcacaggc cgaaactgga ggaagaccta
     9781 actatagaac gactggttat tcacaatcaa atgcgacaac tgatggtcaa cgccaattta
     9841 cgttgaatgg tcaagtgatt caagtattag atatcattaa cccttcaaac ggttatggag
     9901 gacaatccgt tgcaaactct aatgttcgtg ctaatcatag taattctaca gttgttaatg
     9961 taaatgaacc ggcagctaat ggtgcaggtg catttacaat tgatcatgtc gtgaaaaata
    10021 attctacacg taatgcaact gatgctgttt ataaagcaca gttatactta acaccttatg
    10081 gtccaaaaca atatgttgaa catttaaacc aaaatacagg taacactact gatgcaatca
    10141 atatttattt tgtaccaagc gatttagtaa atccaacaat ttcagtaggt aattacgcaa
    10201 atcaccaagt gttctctggg gaaacattta caaatacaat tacagcgaat gataactttg
    10261 gtgtgcaatc tgtaactgta ccaaatacat cacaaattac aggtactgtt gacaataatc
    10321 atcaacatgt ttctgcaaca gcaccaaatg taacatcagc aactactaag acaatcaatt
    10381 tagtggcaac tgatacaagt ggcaatactg caacaacgtc attcaatgta acagttaaac
    10441 cattacgtga taaatatcga gttggaacat catcaacgac tgctaatcct gttagaattg
    10501 ccaatattgc gaataatgcg acagtatcac aagctgatca aactgcaatt attaattcgt
    10561 tgacgtttac tgaaacagta ccaaatagaa gttacgtgag agcaagtgca aatgaaatca
    10621 ctagtaaaac agtgagtaat gtgagtcgta caggaaataa tgctcatgta acagtaacag
    10681 ttacttatca agatggcaca acatcaactg ttactgtacc agttaagcat gtcattccag
    10741 aaattgtttc aaatccacat tacactgtac aaggacaaga tttcccagca ggtaatggtt
    10801 ctagtgcatc agattacttt aaattatcaa atggtagtgc agttccagat gcaacgatta
    10861 cgtgggtaag tggtcaagca cctaataaag acaacactcg tattggtgaa gatatcaatg
    10921 taacagcaca tatcttaatt gatggtgaaa caacaccgat tacgaaaaca tcaacataca
    10981 aggtagtaag ctcagttccg aaacatgtct ttgagacaaa tagaggcgta gtgttcccag
    11041 gtgtatctga cgtgtatgat gcgaaacaat atgttaaacc agttaatgat tcttggacac
    11101 aaaatgcgca acgtatgaat ttccaattta caaattcata tggaccaagt aaagatgtag
    11161 tggggatttc aacaagggat atccgtgtta cttatgataa tcaccaaaca caaattatta
    11221 agattttagc taaagttaag ccagatccac cgagaattga tggaaactct gtgacttata
    11281 aagcaggact tactaatcaa caaataaaga ttaacaacgt acttagtagt tcatctatta
    11341 aattatttaa agcagataat acgccattaa cgattactaa cacaacatat ggtagtggta
    11401 atacagcggt tgtaacggta agtgatgcgt taccaaacgg agaaattaaa gcaagatctt
    11461 cgatttcaat gaacaatgta acgtatacga cacaagatga gcatggtcga gcagttgatg
    11521 tcacaagaac agaatctgta gattctaatg atagtgcaac agtaacagtg acaccacaat
    11581 tacaagcgac atctcaaggt cctgtattta ttaaaggtgg cgacggtttt gatttcggtc
    11641 atgtagaacg atttattcaa aatccaccac atggtgcaac ggttacatgg caagataatc
    11701 cagatacatg gaagaataca gtcggtaaca ctcataaaac tgcggttgta acgttaccaa
    11761 atggtcaagg tacacgtaat gttgaagtgc cagtcaaagt ttatccagtt gccaacgcaa
    11821 aagcaccatc tcgtgatgta aaaggacaaa acttgacaca tggtacaaac gctattgatt
    11881 acattacgtt tgatccaaat acaaacacaa atggtattac agcaacttgg gcaaatagac
    11941 aacaaccaaa taatcaacaa gcaggcgttc aaagcttaaa tgtcgatgta acatatccag
    12001 gcatttcagc tgctaaacga gttcctgtaa ctgtgaacgt atatcaattt gaattccctc
    12061 aaaatagtta tacaacaaca gttggtggca ctttagcaag tggtacacaa gcctctggtt
    12121 atgcacaaat gcaaaatgct acaggtttac caacagatgg atttacgtat aaatggaata
    12181 atgcagcaac aggtacaaat gacgcaaact gggcagctat gaataaaccg aatatggcta
    12241 aagtcgttaa tgcaaaatat gacgtcatct ataacggaca tacatttgca acatctttac
    12301 cagcgaaatt tgtagtaaaa gatgtccaac cagcgaaacc aactgtgact gaaacaactg
    12361 caggagtgat tacaattgct cctggtgcga accaaacagt caatactcat gctggtaatg
    12421 ttacgacata tgctgacaaa ttagttatta aacgtaatgg aagtgttgta acttcattta
    12481 cacgtcgcaa taatacgagt ccatggatga aagaagcatc agcagctaat gtaacaggta
    12541 ttgttggaac taataatggt attactgttg cagcaggtac tttcaatcct gctgatacaa
    12601 ttcaagttgt tgcaacacaa ggtagtggtg aaacaatcag tgacgagcaa cgcagtgatg
    12661 attttacagt tgtcgcacca caaccgaacc aagcgactac taagatttgg caaaatggtc
    12721 atattgatat cacgcctaat aatccatcag gacatttaat taatccaact caagcaatgg
    12781 atattgctta cactgaaaaa gtaggtaatg gtgcagaaca tagtaagaca cttagtgttg
    12841 ttcgtggtca aaataatcaa tggacaattg cgaataagcc tgactatgta acgttagatg
    12901 cacatacagg taaagtgact tttaatgcca acactatcaa gccaaattca gcaattacca
    12961 ttacaccgaa agcaggtact ggtcactcag caagtagtaa tccaagtaca ttaactgcac
    13021 cggcagcaca tactgtcaac acaactgaaa ttgtgaaaga ttatggttca aatgtaactg
    13081 ctgctgaaat taacaatgca gttcaagttg ctaataaacg tactgcaacg attaaaaatg
    13141 gtacagcaat gccaactaat ttagctggtg gtagtacaac gacaattcct gtaacagtga
    13201 cttacaacga tggcagtagc gaagaagtac aagagaccat tttcacaaaa gcggataaac
    13261 gtgagttaat cacagctaaa aatcatttag atgatcatgt gagcacagaa ggtaaaaagc
    13321 caggtacgat tactcaatac aataacgcaa tccataatgc gcaacaacaa attaacgctg
    13381 ctaaaacaga agcgcaacaa gtgattaata atgagcgtgc aacaccacaa caagtttctg
    13441 acgcactaac taaagttcgt gcagcacaaa ctaagataaa tgaagctaaa gcattacttc
    13501 aaaataaaga agataatagc caattagtaa catctaagaa taacttacaa aattctgtga
    13561 accaagtgcc atcaactact ggtatgacgc aacaaagtat tgataactat aatgcgaaga
    13621 aacgtgaagc agaatctgaa attacagcag cacaacgtgt cattgacaat ggtgatgcaa
    13681 ctgcacaaca aatttcagat gaaaaacaac gtgtcgataa cgcgttaaca gcattaaacc
    13741 aagcgaaaca tgatttaact gcagatacac atgccttaga gcaagcagtg caacaattga
    13801 atcgtacagg tacaacgaca ggtaagaaac cagcaagtat tactgcgtac aataattcaa
    13861 tgcatgcgct tcaaagtgac ttaacaagtg ctaaaaatag tgccaacgct attattcaaa
    13921 agccaataag aacagtacaa gaagtgcaat ctgcattaac aaatgtaaat cgtgtaaatg
    13981 accgattaac gcaagctatt aatcaattag tacctttagc tgataatagt gctttaagaa
    14041 ctgctaaaac gaaacttgat gaagaaatca ataaatcagt aactactgat ggtatgacac
    14101 aatcatcaat ccaagcatat gaaaatgcta aacgtgcggg tcaaacagaa tcaacaaatg
    14161 cacaaaatgt tattaataat ggtgatgcga cagatcaaca aattgccgaa gaaaaaacaa
    14221 aagtagaaga aaaatataat agcttaaaac aagcaattgc tggattaaca ccagacttgg
    14281 caccattaca aactgcgaaa actcagttgc aaaatgatat agatcaacca actagtacga
    14341 caggtatgac aagtgcatct gttgcaacat ttaatgaaaa actttcagct gctagaacta
    14401 aaattcaaga aattgatcgt gtattagcat cacatccaga tgttgcaacg attcgacaaa
    14461 acgtgacagc agcgaatgcc gctaaatcag cacttgatca agcacgtaat ggcttaacag
    14521 tcgataaagc gcctttagaa aatgcgaaga ctcaactaca acatagtatt gatacgcaaa
    14581 ctagtacaac tggtatgacg caagactcgg taaatgctta taatgcgaag ttaacggctg
    14641 cacgtaataa gattcaacaa atcaatcaag tattagcagg ttcaccgact gtagatcaaa
    14701 ttaatacaaa tacgtctgca gcaaaccaag ctaaatctga tttggatcat gcacgtcaag
    14761 cgttaacacc agataaaacg ccgcttcaaa ctgcgaaaac gcaattagaa caaagcatta
    14821 accaaccaac tgatacaaca ggtatgacaa ctgcttcgtt aaatgcgtat aaccaaaaat
    14881 tacaagcagc gcgtcaaaag ttagctgaaa ttaatcaagt gttaaatggc aatccaacgg
    14941 tccaaaatat caatgataaa gtgactgaag caaaccaagc taaggatcaa ttaaatacag
    15001 cacgtcaagg tttaacttta gatagacaac cagcgttaaa tacattacat ggtgcatcta
    15061 acttaaacca agcacaacaa aataatttta cgcaacaaat taatgctgct caaaatcatg
    15121 ctgcgcttga aacaattaag tctaacatta cggctttaaa taatgcgatg actaagctaa
    15181 aagaaagtgt tgcggataat aatacgatta aatcaggtca aaattacact gacgcaactc
    15241 aagctaataa acaagcgtat gacaatgcag tcaatgcagc taaaggtgtc attggagaaa
    15301 cgactaatcc aacaatggat gttaacacag tgaaccaaaa agcagcatct gtgaaatcaa
    15361 ctaaagatgc tttagatggt caacaaaact tacagcgtgc gaaaacagaa gcaacaaatg
    15421 cgattacgca tgcaagtgat ttaaaccaag cgcaaaagaa tgcattaaca caacaagtga
    15481 atagtgcgca aaatgttcaa gcagtaaatg atattaaaca aacaactcaa agcttaaaca
    15541 ctgcaatgac aaacttaaaa catggtgttg ctaatcataa ccaagtccta caaagtgata
    15601 attatgtcaa cgcagatact aataagaaaa atgattacaa caatgcatac aaccatgcga
    15661 atgacattat taacggtaat gcacaacatc cagttataac accaagtgat gttaacaatg
    15721 ctttatcaaa tgtcacaagt aaagaacaag cattgaatgg taatacaaaa ttaaatgctg
    15781 cgaagcaaga agcgaacact gcattaggtc atttaaacaa tttgaataat gcacaacgtc
    15841 aaaacctaca atcacaaatt aataatgcgc atcaaattga cgcagttaat acaattaagc
    15901 aaaatgcaac aaacttaaat agtgcaatgg gtaacttaag acaagctgtt gcagataaag
    15961 atcaagtgaa acgtacagaa gattatgcgg atgcagatac agctaaacaa aatgcatata
    16021 acagtgcagt ttcaagtgcc gaaacaatca ttaatcaaac aacaaatcca acgatgtctg
    16081 ttgatgatgt taatcgtgca acttcagctg ttacttctaa taaaaatgca ttaaatggtg
    16141 atgaaaaatt agcacaatct aaaacagatg ctgcaagagc aattgatgca ttaccacatt
    16201 taaataatgc acaaaaagca gatgttaaat ctaaaattaa tgcagcatca aatattgctg
    16261 gtgtaaatac tgttaaacaa caaggtacag atttaaatac agcgatgggt aacttgcaag
    16321 gtgcaatcaa tgatgaacaa acgacgctta atagtcaaaa ctatcaagat gcgacaccta
    16381 gtaagaaaac agcatacaca aatgcggtac aagctgcgaa agatatttta aatcaagcac
    16441 gtggcgctaa taaaacaaaa gatcaagtta ctgaagcgat gaatcaagtg aattcggcta
    16501 aaaataactt agatggtacg cgtttattag atcaagcgaa gcaaacagcg aaacagcagt
    16561 taaataatat gacgcattta acacctgcac aaaaaactaa tttaacaaat caaattaata
    16621 gtggtactac tgttgctggt gttcatatgg ttcaatcaaa tgccaataca ttagatcaag
    16681 cgatgaatac attaagacaa agtattgcca acaaagatga gactaaagca agtgaagatt
    16741 acttagatgc taataatgat aagcaaacag catataacaa tgcagtagct gctgctgaaa
    16801 cgataattaa tgcgaatagt aatccggaaa tgaatccaag tacgattaca caaaaagcag
    16861 agcaagtgaa tagttctaaa acggcactta acggtgatga aaacttagct acggcaaaac
    16921 aaaatgcgaa aacgtactta aacacattaa cgagtattac agatgctcaa aagaacaatt
    16981 tgattagtca aattactagt gcgacaagag tgagtggtgt tgatactgta aaacaaaatg
    17041 cgcaacatct agaccaagca atgtctaatt tacaaaatgg tattaacaac gaatctcaag
    17101 tgaaatcttc tgagaaatat cgtgatgctg atacaaataa acaacaagag tatgataatg
    17161 caattactgc agcgaaagcg attttaaata aatcgacagg tccaaacact gcgcaaaatg
    17221 cagttgaagc agcattacaa cgtgtcaata ctgcgaaaga tgcattaaat ggtgatgcaa
    17281 aattaattgc agctcaaaac gcagcgaaac aacatttagg tactttaacg catatcacta
    17341 cagcacaacg taatgattta acaaatcaaa tttcacgagc tacaaactta gctggtgttg
    17401 aatctgttaa acaaagtgcg aatagtttag atggcgctat gggtaactta caaacagcta
    17461 ttaacgataa agctgcaaca ttagcaagcc aaaacttctt ggatgctgat gagcaaaaac
    17521 gtaatgcata caatcaagct gtatcagctg ccgaaaccat tttaaacaaa caaaatggac
    17581 cgaatacagc gaaatcagca gttgaacaag cacttaataa tttaaatact gctaaacatg
    17641 cattaaatgg tacgcaaaac ttaaataatg cgaagcaagc agcgattaca gcaatcaatg
    17701 gcgcatctga tttaaatcaa aaacaaaaag atgcattaaa agcacaagct aatggtgctc
    17761 aacgcgtttc taatgcacaa gatgtacaac gtaatgcgac tgagctgaac acggcaatgg
    17821 gcacattaaa acatgccatc gcagataaga cgaatacatt agcaagcagt aaatatgtta
    17881 acgccgatag cactaaacaa aatgcttaca caactaaagt taccaatgct gaacatatta
    17941 ttagcggtac gccaacggtt gttacgacac cttcagaagt aacagctgca gctaatcaag
    18001 taaacagcgc gaaacaagaa ttaaatggtg atgaaagatt acgtgtagca aaacaaaacg
    18061 ccaatactgc tatagatgca ttaacacaat taaatacacc tcaaaaagct aaattaaaag
    18121 aacaagtggg acaagccaat agattagaag atgtacaatc tgttcaaaca aatggacaag
    18181 cattgaacaa tgcaatgaaa ggcttaagag atagtattgc taacgaaaca acagtcagag
    18241 ctagtcaaaa ctatacagac gcaagtccga ataatcagtc aacttataat agcgctgtat
    18301 caaatgcgaa aggtatcatt aatcaaacta acaatccgac tatggaagct agtgcgatta
    18361 cccaagctac aacacaagtg aataatggta aaaatggttt aaacggtgct gaaaacttaa
    18421 gaaatgcaca aaacgctgct aagcaaaact taaatacatt atcacactta acaaataacc
    18481 aaaaatctgc aatctcatca caaattgatc gtgcaggtca tgtaagtgag gtaacagcag
    18541 ctaaaaatgc agcaactgag ttgaatacgc aaatgggtaa cttggaacaa gctatccatg
    18601 atcaaaacac agttaaacaa agtgttaaat ttactgatgc agataaagct aaacgtgatg
    18661 cgtatacaaa tgcggtaagt agagctgaag caattctgaa taaaacgcaa ggtgcaaata
    18721 cgtctaaaca ggatgttgaa gcggctattc aaaatgtttc aagtgctaaa aatgcattga
    18781 atggtgatca aaacgttaca aatgcgaaga atgcagctaa aaatgcatta aataacttaa
    18841 cttcaattaa taatgcgcaa aaacgtgact taacaactaa aattgatcaa gcaacaactg
    18901 tagctggtgt tgaagcggta tctaatacag gtacacaatt gaatacagcg atggctagct
    18961 tgcaaaatgg tattaatgat aaagcgaata ctttagcgag cgaaaactat catgatgctg
    19021 attcagataa gaaaactgct tatattcaag ccgttacgaa cgcagaaaat attttaaata
    19081 aaaatagtgg atcaaattta gataaagctg ccgttgaaaa cgcgttgtca caagtaacaa
    19141 atgcgaaagg tgccttaaat ggtaaccata atttagaaca agctaaatca aatgcaaaca
    19201 ctactataaa cggacttcaa catttaacaa ctgcacaaaa agataaattg aaacaacaag
    19261 tgcaacaagc acaaaatgtt gcaggtgtag atactgttaa atcaagtgcc aacacattaa
    19321 atggtgctat gggtacgtta agaaatagca ttcaagataa tgcggctacg aaaaatggcc
    19381 aaaactatct tgatgctaca gaaagtaaca aaacaaacta taacaatgct gttgatagtg
    19441 ctaatggtgt cataaatgca acaagcaatc caaatatgga tgccaatgca attaaccaaa
    19501 tcgctacaca agtgacatca acgaaaaatg cattagatgg tacacataat ttaacgcaag
    19561 cgaaacaaac agcaacaaat gccattgatg gtgctactaa tttaaataaa gcacaaaaag
    19621 atgcgttgaa agcacaagtg acaagtgcgc aacgtgttgc aaatgtaaca agtatccaac
    19681 aaactgcgaa tgaacttaat acagctatgg gtcaattaca acatggtatt gatgatgaaa
    19741 atgcaacaaa acaaacgcaa aaatatcgtg atgctgaaca aagtaagaaa actgcttatg
    19801 atcaagctgt agctgctgcg aaagcaattt taaataaaca atctggatca aattcagata
    19861 aagcagcagt tgaccgtgct ttacaacaag taacaagtac gaaagatgca ttgaatggtg
    19921 atgcgaaact ggcagaagcg aaagctgcag ctaaacaaaa cttaggtact ttaaaccata
    19981 ttacgaatgc acaacgtact gcgttagaag gtcaaatcaa tcaagcgacg actgttgatg
    20041 gcgttaatac tgtaaaaaca aatgccaata cattagacgg cgcaatgaat agcttacaag
    20101 gttcaatcaa tgataaagat gcgacattaa gaaatcaaaa ttatcttgat gcagatgaat
    20161 caaaacgaaa tgcttatact caagctgtca cagctgctga aggcatttta aataaacaaa
    20221 ctggtggtaa cacatctaaa gcagacgttg ataatgcatt aaatgcagtt acaagagcga
    20281 aagcggcttt aaatggtgct gaaaacttaa gaaatgcgaa aacttcagca acaaatacga
    20341 ttaatggttt acctaactta acacaattac aaaaagacaa cttgaagcat caagttgaac
    20401 aagcgcaaaa tgtagctggt gtaaatggtg ttaaagataa aggtaataca ttaaatactg
    20461 ccatgggtgc attacgtaca agtatccaaa atgataatac gacgaaaaca agtcaaaatt
    20521 atcttgatgc atctgatagc aacaaaaata attacaacac tgctgtaaat aatgcaaatg
    20581 gtgttattaa tgcaacgaac aatccaaata tggatgctaa tgcgattaat ggcatggcaa
    20641 atcaagtcaa tacaacaaaa gcagcgttaa atggtgcaca aaacttagct caagctaaaa
    20701 caaatgcgac gaacacaatt aacaacgcac atgacttaaa ccaaaaacaa aaagatgcat
    20761 taaaaacaca agttaacaat gcacaacgtg tatctgatgc aaataacgtt caacatacag
    20821 ctactgaatt gaacagcgcg atgacagcac ttaaagcagc taatgctgat aaagaacgaa
    20881 caaaagcgag cggtaattat gtcaatgctg atcaagaaaa acgtcaagcg tatgattcaa
    20941 aagtgacgaa cgctgaaaat attattagtg gtacaccgaa tgcaacatta acagccaatg
    21001 acgtaaatag tgcgacttca caagtaaatg cagctaaaac agcattaaat ggtgataaca
    21061 acttacgtgt tgcaaaagaa aatgccaaca atacaattga tggcttatca caattgaata
    21121 atgcacaaaa agcaaaatta aaagaacaag ttcaaagtgc aactacttta gatggcgttc
    21181 aaactgttaa aaatagttct caaacgttga atacagcgat gaaaggctta agagatagta
    21241 ttgcgaatga ggcaacaatt aaagcaggtc aaaactacac tgacgcaagt ccaaataatc
    21301 gtaacgagta cgacagtgca gttactgcag caaaagcaat cattaatcaa acatcgaatc
    21361 caacgatgga accaaacact attacgcaag caacatcaca agtaacaact aaagaacagg
    21421 cattaaatgg tgcgcaaaac ttagctcaag ctaagacaac agcgaaaaat aacttgaata
    21481 acttaacatc aattaacaat gcacaaaaag acgcgttaac gcatagcatt gatggtgcaa
    21541 caacagtagc tggtgtaaat caagaatctg ccaaagcaac agaattaaat aacgcaatgc
    21601 atagtttaca aaacggtatc aatgatgaaa cacaaactaa acaaactcag aaatatcttg
    21661 atgccgagcc aagtaagaaa tcagcttatg atcaagcagt tactgcagca aaagcaattt
    21721 taacaaaagc tagtggtcaa aatgtagaca aagcagcagt tgaacaagca ttacaaaatg
    21781 tgaatagtac taagacggcg ttgaacggtg atgctaaatt aaatgaagct aaagccgctg
    21841 cgaaacaaac gttaggtaca ttaacgcaca ttaataatgc acaacgtaca gcgttagata
    21901 atgaaattac acaagcaaca aatgttgaag gtgttaatac agttaaagcc aaagcgcaac
    21961 aattagatgg tgctatgggt caattagaaa catcaattcg tgataaagac acgacgttac
    22021 aaagtcaaaa ttatcaagat gctgatgatg ctaaacgaac tgcttattct caagcagtaa
    22081 atgcagcagc aactatttta aataaaacag ctggcggtaa tacacctaaa gcagatgttg
    22141 aaagagcaat gcaagctgtt acacaagcaa atactgcatt aaacggtatt caaaacttag
    22201 aacgtgcgaa acaagctgct aacacagcga ttacaaatgc ttcggactta aatacaaaac
    22261 aaaaagaagc attaaaagca caagtaacaa gtgcaggacg cgtatctgca gcaaatggtg
    22321 ttgaacatac tgcgactgaa ttaaatactg cgatgacagc tttaaaacgt gccattgctg
    22381 ataaagctga gacaaaagct agtggtaact atgtcaatgc tgatgcgaat aaacgtcaag
    22441 catatgatga aaaagtgaca aatgcagaaa acatcgttag tggtacacca acaccaacgt
    22501 taacaccgtc agatgttaca aatgcagcaa cacaagtaac gaatgcgaag actcagttaa
    22561 acggtaatca taatttagaa gtagcaaaac aaaatgctaa cacagcaatt gaaggcttaa
    22621 gttctttaaa tggtccgcaa aaagcaaaac ttaaagaaca agtgggtcaa gcgacgacgt
    22681 tgccaaatgt tcaaactgtt cgtgataatg cacaaacatt aaacactgca atgaaaggtc
    22741 tacgagatag cattgcgaat gaagcaacga ttaaagcagg tcaaaactac acggatgcaa
    22801 gtcaaaacaa acaaactgac tacaacaatg cagttactgc agcaaaagca atcattggtc
    22861 aaacatcaaa tccaacaatg aatgcgcaag aaatcaatca agcgaaagac caagtgacag
    22921 ctaaacaaca agcattaaat ggtcaagaaa acttaagaac tgcgcaaaca aatgcgaagc
    22981 aacatttgaa tggcttaagt gacttaactg acgctcaaaa agatgcagcg aaacgtcaaa
    23041 tcgagggtgc aacgcatgtt aatgaagtaa cacaagcaca aaataatgcg gatgcattaa
    23101 atacagctat gacgaacttg aaaaatggta ttcaagatca aaatacgatt aagcaaggtg
    23161 ttaacttcac tgatgcagat gaagcgaaac gtaatgcata tacaaatgca gtgacgcaag
    23221 ctgaacaaat tttaaataaa gcacaaggtc caaatactgc aaaagacaat gttgaatctg
    23281 cattacaaaa tgtacaacgt gctaaaaacg aattgaatgg taatcaaaat gttgaaaatg
    23341 ctaaaacaac tgcgaaaaat gcattgaata acctgacatc aattaataat gcacaaaaag
    23401 aagcgttgaa atcacaaatt gatagtgcga caacagttgc aggtgtaaat caagtgtcta
    23461 caacggcatc tgaattaaat acagcaatga gcaacttaca aaatggaatt aatgatgaag
    23521 cagctacaaa agcagctcag aaatatactg atgcagatag agataaacaa acagcatata
    23581 atgatgctgt aacagcagct aaaacgttat tagataaaac agctggttca aatgacaata
    23641 aagcagccgt tgaacaagca ttacaacgtg tgaatactgc taagtcagca ttaaatggtg
    23701 atgcgcgatt aaatgaagct aagaacacag ctaaacaaca attagcgaca atgtcacatt
    23761 taactgatgc tcaaaaagca aacttaacag aacaaattga acgcggtacg actgttgcag
    23821 gtgttcaagg tattcaagct aatgccggta ctttagatca agcaatgaat caattaagac
    23881 aaagtattgc ttctaaagat gcgactaaat caagcgaaga ttatcaagac gcgaatgcag
    23941 atttacaaaa tgcatacaac cgtgcagtat ctgatgctga gggtattatt agtgcaacga
    24001 ataatcctga aatgaatcca gatacgatta atcaaaaagc gagtcaagtg aacagtgcga
    24061 agtctgcatt gaacggtgat gaaaaattag cagcagcaaa acaaactgcg aaatcagata
    24121 tcggtcgttt gacagacttg aacaatgcac aacgaactgc ggcaaatgct gaagtggatc
    24181 aagcaccaaa ccttgcagct gtcacagcgg ctaaaaataa agcaacatca ttaaacacag
    24241 cgatgagtaa tttgaaaaat gcacttgctg aaaaggataa tacgaaacgt agtgtcaatt
    24301 acacagatgc ggatcaacct aaacaacaag cgtatgatac tgcagttaca caagcagaag
    24361 gaattactaa tgctaatggc agcaacgcga atgaaacaca agttcaagca gtgcttaacc
    24421 aattgaatca agctaaaagc gatttgaatg gtaacaacaa agttgctcaa gctaaagaag
    24481 cagcgaaacg tgcattagct tcatatagta acttgaataa tgcgcaatca actgcagcaa
    24541 ctagtcaaat tgacaatgca acgacagtag caggtgtaac tgctgcacaa aatactgcta
    24601 acgaattaaa tacagcaatg ggtcaacttc aaaatggtat aaatgaccaa aacactgtaa
    24661 aacaacaagt gaactttaca gatgctgacc aaggtaagaa agaagcttac acaaatgctg
    24721 ttacgaatgc gcaaggtatt ttagataaag caaacggtca aaatatgaca aaagcacaag
    24781 ttgaagctgc attaaatcaa gtaacgactg ctaagaatgc tttaaacggt gatgcaaatg
    24841 taagacaagc aaaatcagat gcgaaagcaa acttaggtac attaacacat ttaaataatg
    24901 cgcaaaaaca agatttaaca tcacaaatcg aaagcgcgac tacagttaac ggtgtaaatg
    24961 gtgtaaaaac gaaagcacaa gacttagatg gtgcaatgca acgattacaa tcagctatcg
    25021 caaataaaga tcaaactaaa gcaagcgaaa actacatcga cgcagatcca actaagaaaa
    25081 cagcatatga caatgccatt acacaagctg aatcttactt aaataaagat cgcggtgcga
    25141 ataaagataa gcaagctgtg gaacaagcaa ttcaaagtgt aacaactact gaaaatgctt
    25201 taaacggtga cgcaaattta caacgcgcta aatctgatgc tacacaagct atcgataact
    25261 tgacacattt gaatacacca caaaaaacag cattaaaaca acaagtgaat gctgcgcaac
    25321 gtgtatcagg tgtaactgat ctgaaaaata gtgctacatc acttaataat gcgatggatc
    25381 aattaaaaca atcaattgct gatcatgaca caattgtagc tggtggtaat tacactaacg
    25441 caagtcctga taagcaaggt gcttacacag atgcatataa tgctgcgaaa aacattgtaa
    25501 atggttcacc taatgtgatt acaaatgcag cagatgttac agcggcaaca caacgtgtca
    25561 ataatgctga aacaggttta aacggtgata caaacttagc aactgcgaag caacaagcta
    25621 aagatgcatt acgtcaaatg acacatttat ctgatgcgca aaaacaaagt attactggtc
    25681 aaattgatag cgcgacacaa gtaactggcg ttcaaagtgt aaaagacaat gcaacaaatc
    25741 ttgacaatgc aatgaatcaa cttcgaaata gtattgcgaa taaagatgaa gtaaaagcga
    25801 gtcaaccata tattgatgca gatacagata aacaaaatgc atacaataca gctgttacaa
    25861 gtgctgaaaa tatcattaat gcaacgagtc agccgacact tgatccatct gcagtaacac
    25921 aagcagcaaa tcaagtgaac actaacaaaa ctgcgcttaa tggtgcacaa aacttagcaa
    25981 ataaaaagca agaaacaact gctaacatca accgattaag tcatttaaac aatgctcaaa
    26041 agcaagattt aaatacgcaa gtgacaaatg caccaaatat tagcacagta aatcaagtga
    26101 agactaaagc tgaacaatta gatcaagcaa tggaacgttt aatcaacgga atccaagaca
    26161 aagatcaagt gaaacaaagt gttaacttta cagatgcaga tccagaaaaa caacaagcat
    26221 ataccagtgc ggtaactgct gctgaaaata ttattaatca agcaaatggt acaaatgcga
    26281 atcaatcaca agttgaagca gcactttcaa ctgtaacaac tactaaacaa gcgttgaatg
    26341 gtgatagaaa agtaacagat gctaaaaaca atgcaaacca aacattatca acgttggata
    26401 atttaaacaa tgcacaaaaa gctgctgtta ctggaaacat caaccaagcg catactgtag
    26461 ctgaagtaac gcaagccatt caaacagctc aggaactgaa tacagcgatg ggtaacttga
    26521 aaaatagctt gaatgataaa gacactacac ttagcagtca aaacttcact gatgcagatc
    26581 cagagaagaa aaatgcatac aatgaagcgg ttcgaaatgc tgaaaatatt ttaaataaat
    26641 ctacaggtac aaacgtgcct aaagatcaag ttgaagcagc tatgaatcaa gtgaatacta
    26701 caaaagcagc gcttaatggt actcaaaacc ttgaaaaagc taaacaacac gcaaatacag
    26761 caattgacgg tttaagccat ttaacaaatg cacaaaaaga tgcattaaaa caattggtac
    26821 aacaatcgac aactgttgca gaagcacaag gtaatgaaca aaaagcaaac aatgttgatg
    26881 cagcaatgga caaattacgt caaagtattg cagataatgc gacaacaaaa caaaaccaaa
    26941 attatactga tgcaagtccg aataaaaagg atgcgtacaa taatgctgtc acaactgcac
    27001 aaggtattat tgatcaaact acaagtccaa ctttagatcc gactgttatc aatcaagctg
    27061 ctggacaagt aagcacaact aaaaatgcat taaatggtaa tgaaaaccta gaggcagcga
    27121 aacaacaagc gacacaatca ttaggttcat tagacaactt aaataatgcg caaaaacaag
    27181 ctgttactga tcaaattaat ggtgcgcata ctgttgatga agcaaatcaa attaagcaaa
    27241 atgcgcaaaa cttaaatact gcgatgggta acttgaaaca agcgatagct gataaagatg
    27301 ctacgaaagc aacagttaac ttcactgatg cagatcaagc aaaacaacaa gcatataaca
    27361 ctgcagttac aaatgctgaa aatatcattt caaaagctaa tggtggcaat gcaacacaag
    27421 ctgaagttga acaagcaata caacaagtta atgctacaaa acaagcatta aatggtaatg
    27481 ccaacgttca acatgcgaaa gatgaagcaa cagcattaat taataactca aatgacctta
    27541 atcaagcaca gaaagatgca ttaaaacaac aagtacaaaa tgcaacaact gttgctggtg
    27601 taaacaatgt taaacaaaca gctcaagagt taaacaatgc gatgacacaa ttaaaacaag
    27661 gcattgcaga taaagaacaa acgaaagctg atggtaactt tgtcaatgca gatcctgaca
    27721 agcaaaatgc atataaacaa gcagtagaca aagctgaagc attaattagt ggcacacctg
    27781 atgctgtcgt tacacctagt gaaattactg cagcgttaaa taaagttacg caagctaaaa
    27841 atggtttaaa tggtaatacg aacttagcaa cggcgaaaca aaatgttcaa catgctattg
    27901 atcaattgcc aaacttaaac caagcgcaac gtgatgaata cagcaaacaa atcacgcaag
    27961 caacacttgt accaaacgtc aatgctattc aacaagcggc gacaacgctt aatgatgcga
    28021 tgacacaatt gaaacaaggt attgcggata aagcgcaaat taaaggtagc gaaaactatc
    28081 atgatgctga tactgacaag caaacagcat atgacaatgc agtaactaaa gcagaagaat
    28141 tgttaaaaca aacaacaaat ccaacaatgg atccaaatac aattcaacaa gcattaacta
    28201 aagtgaatga tacaaatcaa gcacttaacg gtaatcaaaa attagctgat gccaaacaag
    28261 atgctaagac aacgtttggt acactagatc atttaaatga tgctcaaaaa caagcgttaa
    28321 caactcaagt tgaacaagca cctgatattg caacagttaa taatgttaag caaaatgctc
    28381 aaaatctgaa taatgcaatg actaacttaa gtaatgcatt acaagataaa actgagacat
    28441 taaatagcat taattatact gacgcagatc aaggtaagaa agatgcttat acaaatgcgg
    28501 ttgcacatgc agaaggtatt ttatctaaag caaatggcag caatgcaagt caaactgaag
    28561 tcgaacaagc gatgcaacgt gtcaacgaag cgaaacaagc attgaatggt aatgacaatg
    28621 tacaacgtgc gaaagatgca gcaaaacaag tgattacaaa tgcaaatgat ttaaatcaag
    28681 cacaaaaaga tgcgttaaaa caacaagtcg atgctgcgca aactgttgca aatgtaaaca
    28741 cgattaagca aacagcacaa gatttaaacc aagcaatgac gcaattgaaa caaggtattt
    28801 cagataaaga ccaaacaaaa gcaaatggta actttgtcaa tgcagatcct gaaaagcaaa
    28861 atgcttataa taatgcggta gcacatgctg aacaaatcat tagcggtaca ccaaatgcaa
    28921 acgtagatcc acaacaagtg gctcaagcgt tacaacaagt gactcaagct aaggatgatt
    28981 taaacggtaa ccacaactta caagttgcta aagacaatgc gaatacagca attgataact
    29041 tgccaaactt aaatcaacca caaaaatcag cattaaaaga tcaagtatca catgcagaac
    29101 ttgttacagg tgttaatgct attaagcaaa acgctgatgc attaaataat gcaatgggta
    29161 cattgaaaca acaaattcaa gcgaatagtc aagtaccaca gtcagttgac tttacacaag
    29221 cggatcaaga caaacaacaa gcatataaca atgcggctaa ccaagcacaa caaatcgcaa
    29281 atggcacacc aacacctgta ttgacgcccg atacagtaac acaagcagtt acaactatga
    29341 atcaagcgaa agatgcatta aacggtgatg aaaaattagc acatgcgaaa caagaagctt
    29401 tagcaaatct tgatacgtta cgcgacttaa atcaaccaca acgtgatgca ttaagaaacc
    29461 aaatcaatca agcacaagcg ttagctacag ttgaacaaac aaaacaaaat gcacaaaatg
    29521 tgaatacagc aatgggtaac ttgaaacaag gtattgcaaa caaagatact gtcaaagcaa
    29581 gtgagaacta ccacgatgct gatgccgata aacaaacggc atatacaaat gcagtgtctc
    29641 aagcggaagg tattatcaat caaacgacaa atccaacgct aaacccagat gaaataacac
    29701 gtgcattaac tcaagtgact gatgctaaaa atggtttaaa cggtgatgct aaattagcaa
    29761 ctgaaaagca aaatgctaaa gatgctgtca atgcaatgac gcatttaaac gatgcacaaa
    29821 aacaagcatt gaacggtcaa attgatcaat cacctgaaat tgcgacagta actcaagtta
    29881 aacaaacagc aacgagccta gatcatgcaa tggatcaatt atcacaagct attaacgata
    29941 aggctcaaac attagcggat ggtaattact taaatgcaga tcctgacaaa caaaatgctt
    30001 ataaacaggc agtagcaaaa gctgaatcat tacttgataa acaaagcggt actaatgaag
    30061 tacaagcaca agttgaacgc atcactaatg aagtgaacac agcgaaacaa gcattaaatg
    30121 gtaatgacaa tttggcaaat gcaaaacaac aagcgaaaca acaattggcg aatttaactc
    30181 atttaaatga tgcacaaaaa caatcatttg aaagtcaaat tacacaagcg ccacttgtta
    30241 cagatgtcac tacaattaat caaaaagcac aagcgttaga tcatgcaatg gaattattaa
    30301 gaaatagtat tgcggataac caagcgacat tagcgtctga agattatcat gatgcaactg
    30361 cgcaaagaca aaatgactat aaccaagctg taacagctgc taataatatc attaaccaaa
    30421 caacatcacc tacgatgaat ccagatgaag ttaatagtgc aacgacacaa gtgaataaca
    30481 cgaaagttgc attagatggt gatgaaaacc ttgcagcagc taaacaacaa gcgaacaata
    30541 gacttgatca attagatcat ttgaataatg cgcaaaagca acagttacaa tcacaaatta
    30601 cgcaatcatc tgatattgct gcagttaatg gtcacaagca aacagcagaa tctttaaata
    30661 cagcaatggg taacttaatt aatgcgattg cagatcatca agcagtagaa caacgtggca
    30721 actatgtcaa tgcagatact gataaacaaa ctgcttatac tactgcggta aatgaagcag
    30781 aagcaatgat taataaacaa actggccaaa atgctaacca aacagaagtg gaacaagcta
    30841 ttaataaagt tcaaacaaca cttcaagcgt taaatggaga tcataattta caagtggcta
    30901 aagcaaatgc gacgcaagca attgatgctc tatcaagttt aaatgatcct caaaaaacag
    30961 cattaaaaga ccaagtcaca gctgcaacat tagtgactgc agttcatcaa attgaacaaa
    31021 atgcgaatac gcttaaccaa gcaatgcatg gcttaagaga aagtattcaa gataacgcag
    31081 caactaaagc aaatagcatt tatatcaatg aagatcaacc agagcaacaa aactatgatc
    31141 aagctgttca agccgcaaat aatattatca atgaacaaac tgcaacatta gataataatg
    31201 cgattaatca agcagctaca actgtgaata caacgaaagc agcattacat ggtgatgtga
    31261 aattacaaaa cgataaagat catgctaaac aaacggttag tcaattagcg catctaaaca
    31321 atgcacaaaa acatatggaa gatacgttaa ttgatagtga aacaactaga acagccgtta
    31381 accatgattt gactgaagca caagcattag atcaacttat ggacgcatta caacaaagca
    31441 ttgcagacaa agatgcaaca catgcgagca gtgcctatgt caatgcggaa ccgaataaaa
    31501 aacaagccta tgatgaagca gttcaaaatg ctgagtctat cattgccgga ttaaataatc
    31561 caactatcaa taaaggtaat gtatccagtg cgactcaagc agtaacatca tctaaaaatg
    31621 cattagacgg tgttgaacga ttagcccaag ataagcaaac agctggaaat tcattaaatc
    31681 atttagatca attaacacca gcacaacaac aagcgctaga aaatcaaata aataatgcaa
    31741 caactcgtga taaagtggct gaaattattg cacaagctca agcattaaac gaagcaatga
    31801 aagcattaaa agaaagtatt aaagatcaac cacaaacaga agcaagtagt aaatttatta
    31861 atgaagatca agcgcaaaaa gatgcttata cacaagcggt acaacacgcg aaagatttga
    31921 ttaacaaaac aactgaccct acattagcta aatcaatcat tgatcaagct acacaggcag
    31981 tgactgatgc taaaaacaac ttacatggtg atcaaaaatt agcgcaagat aagcaacgtg
    32041 caacagaaac gttaaataac ttatctaact tgaatacacc acaacgtcaa gcactagaaa
    32101 atcaaatcaa taatgcagca actcgtggcg aagtagcaca aaaattaact gaggcacaag
    32161 cacttaacca agcaatggaa gcattacgta atagcattca agatcaacag caaacggaag
    32221 cgggtagcaa atttatcaat gaagataaac cgcaaaaaga tgcttaccaa gcagcagttc
    32281 aaaatgcaaa agatttaatt aatcaaacta gcaatccaac gcttgataaa gcacaagttg
    32341 aacaattgac acaagctgtt aaccaagcta aagataacct acacggtgat caaaaacttg
    32401 cagacgataa acaacatgcg gttactgatt taaatcaatt aaatggttta aataatccgc
    32461 aacgtcaagc acttgaaagt caaataaaca atgcagcaac tcgtggcgaa gtagcacaaa
    32521 aattagctga agcaaaagca cttgatcaag caatgcaatc attacgtaat agtattcaag
    32581 atcaacaaca aactgaatct ggtagcaagt ttatcaatga agataaacca caaaaagatg
    32641 cttaccaagc agcagttcaa aatgcaaaag atttaattaa ccaaacaggt aatccaacac
    32701 tcgacaaatc acaagtagaa caattaacac aagcagtaac aactgcaaaa gataatctac
    32761 atggtgatca aaaactagct cgtgatcaac aacaagcagt aacgactgtt attgcattac
    32821 caaacttaaa tcatgcacaa caacaagcat taactgatgc aattaatgca gcgcctacaa
    32881 gaacagaggt tgcacaacat gttcaaacgg ctactgaact tgaccatgcg atggaagcat
    32941 taaagaataa agttgatcaa gttaacacag ataaggctca accaaattac actgaagcat
    33001 caacggataa aaaagaagca gtggatcaag cgttacaagc tgcacaaagc attacagacc
    33061 ctactaatgg ttcaaatgca aataaagatg ctgtagaaca ggcgttaact aagcttcaag
    33121 aaaaagaaaa tgagttaaat ggtaatgaga gagtcgctga agctaaaaca caagcgaaac
    33181 aaaatattga ccaattaaca catttaaatg ctgatcaaat cgcaactgct aaacaaaaca
    33241 ttgatcaagc gacgcaactt caaccaatcg ctgaattagt agatcaagca acacaattga
    33301 accaatcaat gggtcaatta caacaagctg ttaatgatca tacaaatgtt gaacaaacag
    33361 ttgattatac gcaagcggat tcagataagc aaaaggctta taaacaagcg attgctgatg
    33421 ctgaaaatgt attaaaacaa aatgcgaata agcaacaagt agatcaagcg cttcaaaaca
    33481 ttttaaatgc aaaacaagca ttaaatggtg atgaacgtgt tgaacttgct aaatcaaatg
    33541 gtaaacatga tatagatcaa ttaaatgcat taaacaatgc acaacaagat ggatttaaag
    33601 gtcgtattga tcaatcaaat gacttaaatc aaatccaaca aattgttgat gaagctaagg
    33661 ctctaaatca tgcaatggat caattgtctc aagaaattac tggcaatgaa ggacgtacga
    33721 aaggtagcac gaactatgtc aatgcagata cacaagttaa acaagtgtat gatgaagcgg
    33781 ttgataaagc gaaacaagca cttgataaat cgacaggtca aaacttaact gcagaacaag
    33841 ttatcaaatt aaatgatgca gtcactgcag ctaaggaagc attaaatggt gaagaaagac
    33901 taaataaccg taagtctgaa gcattacaaa gattggatca attaacacat ctaaacaatg
    33961 ctcaaagaca attagcaatc caacaaatta ataatgctga aacgctaaat aaagcatctc
    34021 gagcaattaa tagagctgtt caattagatg atgcaatggg tgcagtacaa cagtatattg
    34081 atgaacatca tcttgatgtc attagcagta caaattacat taatgcagac gataacttga
    34141 aagcaaatta tgacaacgcc attgcgaatg cagcacatga gttagataaa gtacaaggta
    34201 gtgcaattgc gaaagctgaa gcagagcaat tgaaacaaaa tattatcgat gctcaaaatg
    34261 cattaaatgg agatcaaaat cttgcaaatg ctaaagaaaa agcaaatgca tttgttgata
    34321 cattaaatgg cttaaatcaa caacaacaag atcatgcaca tcaagcaatt aacaatgcag
    34381 atactgtagt aggtataaca gatattgtta ataatcaaat tgacttaaat gatgcaatgg
    34441 aaacattgaa acagttagtt gacaatgaaa ttccaactgc agaacaatct gtaaattacc
    34501 aaaacgctga cgacaatgct aaatcaaact ttgatgatgc aaaacgttta gcaaatacat
    34561 tgcttaatag tgataacaca aatgtgaatg acattaatgg tgcaatccaa tcagtaaacg
    34621 atgcaatcca aaatcttaac ggtgatcaac gtttgcagga agctaaagac gaagcaattc
    34681 aatcgattaa tcaagcatta gcagataaat tgaaagaaat cgaagcatca aatgcgacgg
    34741 atcaagacaa acttattgcg aaaaataaag cagaagaact ggcaaacagc atcattaaca
    34801 acattaataa agcaacaagt aatcaagctg taacggaagt tcaaactgct ggcaaccaag
    34861 cgattgaaca agttcacgca aatgaaatac cgaaagctaa aattgatgca aacaatgatg
    34921 ttgataatcg agttcaagca ttaattgatg aaattgatcg aaatccaaat ctaacagata
    34981 aggaaaaaca agcgcttaaa gatcgaatca atcaaatact gcaacaaggt cataacgaca
    35041 taaacaatgc gatgactaaa gaagaaattg aacaagcaaa agaacgcctt gcgcaagcat
    35101 tacaagacat taaagattta gtgaaagcta aagaaaatgc gaaacaagat gttgataacc
    35161 gagtgcaagc attaattgat gaaattgatc gaaatccaaa tctaacagac aaggaaaaac
    35221 aagcgcttaa agatcgaatc aatcaaattc tacaacaagg tcataacgac attaacaatg
    35281 cgatgactaa agaagaaata gaacaagcaa aagaacgcct tgcacaagca ttacaagaca
    35341 ttaaagattt agtgaaagct aaagaagatg cgaaacaaga tgttgataaa caagttcaag
    35401 ctttaattga cgaaatcgat caaaatccaa atctaacaga taaggaaaaa caagcactta
    35461 aagatcgtat taatcaaata ttgcaacaag gtcataacga cataaacaat gcgctgacta
    35521 aagaagaaat tgagcaggca aaagcacaac ttgcacaagc attgcaagac atcaaagatt
    35581 tagtgaaagc taaagaagat gcgaaaaatg caataaaagc cttagctaat gcgaagcgtg
    35641 atcaaatcaa ttcaaatcca gatttaacac ctgagcaaaa agcaaaagca cttaaagaaa
    35701 ttgacgaagc tgaaaaacga gcattacaaa atgttgaaaa tgcacaatct aaagatcaat
    35761 taaatcaaag attaaattta ggcttagaag acattagaaa tacgcatgta tgggaagttg
    35821 atgaacaacc tgctgtaaat gatatttctg aagcgacgcc tgaacaaata cttgttaatg
    35881 gtgaactaat cgtacaccgt gatgacatca ttactgaaca agatatttta gcgcacataa
    35941 acttaattga tcagcttaaa gcagaagtta ttgatacacc atcaactgca acaatttctg
    36001 atagtttaac agcaaaagtt gaagtaacat tgcttgatgg atcaaaagtg attgttaatg
    36061 ttcctgtgaa agtagtagaa aaagaattga cagtagtcaa acagcaggcg attgaatcaa
    36121 ttgaaaatgc ggcacaacaa aagattaatg aaatcaataa tcacgcaaca ttaacacctg
    36181 aacaaaaaga agctgcgatt gcggaagtta ataagcttaa acaacaagct attgagcaaa
    36241 ttaacaacgc agcagatgtt catacggtgg aagaagttca aaaccaagaa caggctcata
    36301 ttgaacaatt taatccagat caatttacaa ttgaccaagc taaatcaaat gctattaaat
    36361 caattactga tgctatccaa catatgattg atgaaattaa tgctagtaag gatttaactg
    36421 ataaagagaa gcaagaagct atatctaaat taaatcagtt aaaagatcaa tcaattcagg
    36481 cgattcaacg tgcgcaaagc atcgttgaaa ttacgcaaca attagatcaa tttaaagcgc
    36541 aactgaaagc ggctaatcca tttgctaaag aattggaaaa ccgtaaaaaa gcagcgattt
    36601 caaaaattaa agatatttct acagataaaa ttgatcgcat tagaaatagt acaattggaa
    36661 ctgctgaaga aagacaagca gcaatgaatc gaattaacga aattgtgctt gaaacaatta
    36721 gagatattaa taatgcgcat acactacaac aagttgaggc tgcattgaat aatggtatcg
    36781 aacgaatttt agcagtacaa attgtaacat ctgatcatag caaacattca tcaaattcag
    36841 atggtcaatc taatagtcat ttacacgttg gttatggcac tgtaaatcat ccattcaata
    36901 gttcgcctat tggacataag aagaaattgg atcaagatga tgaaatcgat ccacttcata
    36961 tgcgtcattt cggtgaccga attggtaatg ttatcaaaaa cgcacttggc gttgttggta
    37021 tttctggatt gcttgctagc ttctggttct tcattgcgaa acgtcgtcgt aaagaggatg
    37081 aagaggaaga attggaaatt agagataaca gcaaagacaa gaagaaaggt tcaatagaag
    37141 gtactaaaca tttaccactt ttatttgcga aacgtcgcag aaaagaagat gaagaagatg
    37201 ctattgttga agaaaaagat tcgctaaaca atgacgagtc actcgataaa gttaaacata
    37261 cgccgttctt cttacctaaa cgtcgtcgca aagaagatga agaagatgtg gaagttacaa
    37321 atgaaaacac agatgaaaaa gtgttgcaag ataacgaaca ttcaccggtc ttaattgcaa
    37381 aacgccgtcg cgataaagag gaagatgttg aaacaacaac tagtattgaa tctaaagatg
    37441 aggatgtacc tttattattg gctaaaaaga aagatcaaaa agataaccaa tccaaaggga
    37501 aaaagtcggc atcaaaaaaa acttctaaaa aggtagcagc taaaaagaag aaaaagaaat
    37561 ctaagaaaaa taaaaaataa tttgtttctt cgataaaaag aggagcaccg attgaaatta
    37621 tatcagtcgg tgctcctttt atttgttctt tttaattaat ttatacaatg cttgctgtgc
    37681 gtgttgattc gcttctttgt tttgttctct cggtatccat ttaacaaata ataaatcaaa
    37741 atctttttca aatatttcca attgatcaaa ataaggttta aattttgcat ttttaacata
    37801 accagcgtta acactatccg caattaactt agagtcagtg tacaacaacg cattgctaac
    37861 gtttaattca cgggcgtgtt tcaatgcata aatacatgca gcccattctg cagtgtggtt
    37921 atccatttcg cctaactcat gtgtatatgt ataatgctgc tcatcttctt tgattacaat
    37981 ggcacatgta cttatgcctg gatttccttt cgtcgcagca tcaaaattta ttttcgccat
    38041 aataaaccta ctttctattc aatacttagt taaagttact attactgtaa tacaaaatat
    38101 gttgggtaat ccattaaaaa acacgcatca cttaaataag taacacgtgt cgtaaaataa
    38161 gctatgaatt tgaggcgtta tctgattcaa tttcagttgt agatattttc ctaattgaaa
    38221 agcactacat tactgtttct gaaaaggtct caaattaact aacgaatact attatttaat
    38281 agccttgatc taacttatca attttacctt tacgataaaa tgatttagca atatatccaa
    38341 atggtacatt gaaaactgtt gaagctaatt ttagtacgta cgttgtaata aatatttcaa
    38401 atacaaccgt accaggtaaa cttccgataa atgcgatagc tacaaataaa gctgtatcaa
    38461 ttattgagct taaaaatgta ctaccatatg cacggatgaa aaacgtttta tcagaactaa
    38521 atactttttt aattagtgaa aagataaata catcaatatg ttgaccaata atatatgcga
    38581 cgattgagcc taaagcaata cgtggcacaa catcaaagat tgcgtgtaat gctttttgtg
    38641 ccatatcttc tggtgcagga ataaaatgta atgacaattg cataacaata atcataatta
    38701 atgttgatga aaagcctaac caaactgctc tttttgcaac tctacgccca taaatatcgt
    38761 ttaatatatc tgttgctaaa taaatagaag caaacatgac attacctaaa gttgcagaaa
    38821 taccaaaaat ttctactgtt ttaattactt gaatgttggc aatgattgtg ccaatagcaa
    38881 cccatgcaat taaaccttgc tttccgaaaa aacgatacat taacaccata agaacgaatg
    38941 tcacaataaa tgtaacaagt cctaaaattt cattatacat attaaaatgt cctcctaaat
    39001 tttgatcatg cgggtgttta gaaaccgctc aataaataac ttactcattt tactgaaaaa
    39061 tcgtttgcga tgcaatagca tttgaatata aatacatttt acgtatagaa ttgtaaaagg
    39121 tttcattcaa atcttagggt caaaaatgtt ataatatttt tatgtcaaat ttaaaacagt
    39181 aacacttatt tacaaggttg caatattttg aagtaataaa ggaagtgtcg cgtattttaa
    39241 ctttttcaga gcaaaatgca ctcgcgaaaa tagatgattt aatgaatact tattgcaatc
    39301 aatgtccaat caaaactcgt ctgcgtaaat tagaggggaa aacgaaggcg catcattttt
    39361 gtatcaatga gtgttcaata gggaaagaaa taaaacaatt aggaaatgaa cttcaatagg
    39421 aggaagtcaa atgcaaatta tatcaatatc agaaacaccg aaccacaaca caatgaagat
    39481 tacacttagt gaaagcagag aaggtatgac atcagatacg tatactaaag ttgatgattc
    39541 acagccagca tttattaatg acatcttaaa ggttgaaggt gttaaatcaa ttttccatgt
    39601 tatggacttt atttcagtag ataaagaaaa tgacgcaaat tgggaaacag tattgccaaa
    39661 agtagaggct gtattcgaat aaatttttca tcaactagta ttcgggggga ataaagtata
    39721 tggaaatttt acgtatagag ccaacgccaa gtccaaatac aatgaaagtt gttttgtcat
    39781 atacaagaga agacaagtta tctaatactt ataaaaaagt agaagaaaca caaccagaat
    39841 ttataaatca gttgttatct atagatggta tcacttccat ttttcatgtc atgaacttct
    39901 tagctgttga taaggcacca aaagctgatt gggaagtcat attacctgat attaaagctg
    39961 ctttttctga tgcgaatcag gtattagaat ctgtaaatga acctcaaatt gacaatcatt
    40021 ttggtgaaat taaagctgaa ctattaactt ttaagggtat accgtatcaa attaagctaa
    40081 cttctgctga ccaagaatta agagaacaat taccacaaac atatgttgat catatgacgc
    40141 aagcgcaaac agcacatgac aatattgttt ttatgcgtaa atggctagat ttagggaatc
    40201 gctatggaaa tattgaggaa gtaatggatg gtgtcctaga agaagtgcta gctacctatc
    40261 cagaatcaca gttacccgta ttggtaaaac atgctttaga agaaaatcac gcaactaata
    40321 attatcattt ttatcgacat gtctctttgg atgaatatca tgcgactgat aattggaaga
    40381 ctcgattacg aatgttaaac cattttccaa aaccgacttt tgaagatata ccgctgcttg
    40441 atttagcttt atctgatgaa aaagtaccgg ttagacgtca agcgattgta ttattaggta
    40501 tgattgaaag taaagaaatt ttaccgtatt tatataaggg gcttcgtgat aaaagtcctg
    40561 ctgtaagaag aacagcaggg gattgcataa gcgatttagg gtatctagag gcactaccag
    40621 aaatggtact actattagat gatccacaga aaatcgttag gtggcgtgct gctatgttta
    40681 tctttgatga aggtaatgca gagcagcttc ccgcacttaa agcccatatt aatgacaatg
    40741 cgtttgaagt taaattacaa attgaaatgg ccatatcccg tattgaaaac ggagatgaag
    40801 cacttggttc agtatggaaa caaatggcaa acagaacaat ttaattggag gaattaaata
    40861 tgaatgcata tgatgcttat atgaaagaaa ttgcgcaaca aatgcgtggc gaattaactc
    40921 aaaatggttt tacaagttta gaaacgagcg aagcagtatt agagtatatg aaccaagtaa
    40981 atgctgatga cactactttt gtagttatta actctacatg cggctgtgca gctggattag
    41041 caagaccagc tgcagtagca gtagcaacac aaaatgaaca tagacctaca aatacagtta
    41101 cagtttttgc tgggcaagat aaagaagcaa ctgctacaat gcgagaattc attcagcaag
    41161 caccatctag tccttcgtat gctttattca aaggtcaaga tttagtttat tttatgccta
    41221 gagaatttat cgaaggcaga gatattaatg acattgcaat ggacttaaag gatgcctttg
    41281 acgaaaattg taaatagtac acataaatat aaaggttaac acattttata atattaaaaa
    41341 tggtgtctgt cattgaaaat agagaatata gttgtattcc atttgttata taaagtccgt
    41401 tcttaccaac tatattttct agaaatttaa ctgttttaat aggacatcaa acataatatt
    41461 taaatcatgt gttaacctct tttttaaaat tttttagcat taaagttata gatttgggta
    41521 aacaattagc aattggaaac atatatcacg ttacaatgtg gtaggtactt aatcagcatt
    41581 ttataaatta agtaacggaa tccatgatat taatatcaca ttcctaaaat gagtgataac
    41641 aaaatgctac ataaagttaa attatgtcga actaaatata catactataa ataatgaaaa
    41701 tgaggtgtta tcgcatatgt tgaattcatt tgatgcagca tatcacagtc tttgtgaaga
    41761 agttttagaa ataggaaata cacgaaatga tcgcacaaat acaggtacga tttcgaaatt
    41821 tggtcatcaa cttcgctttg acttatctaa aggatttcca ctattaacga caaagaaagt
    41881 ttcttttaaa ttagtagcaa ccgaattatt atggttcatt aaaggagata caaacatcca
    41941 atacttatta aaatataata ataatatttg gaacgaatgg gcttttgaaa attatatcaa
    42001 atcagacgag tataaaggtc cagatatgac tgattttggg catcgtgcat tgagtgatcc
    42061 tgaatttaac gaacaatata aagaacaaat gaaacaattt aagcaacgta ttcttgaaga
    42121 taatacattt gcgaagcaat tcggtgattt aggaaatgtt tatggtaaac aatggcgaga
    42181 ttgggttgat aaagatggta atcattttga tcaacttaaa acagtaattg agcaaattaa
    42241 gcataatcca gattcaagac gacacatcgt atctgcatgg aatccaacag aaattgacac
    42301 aatggcactt ccgccttgtc ataccatgtt ccagttttat gtccaagatg gtaagttaag
    42361 ttgccagtta taccaacgta gcgcagatat ctttttaggt gtgccattta atatcgcaag
    42421 ctacgcttta ttaacacacc ttattgccaa agaatgtgga cttgaagtag gagaatttgt
    42481 gcatacattt ggagatgcac atatttattc aaatcatatt gatgcgattc aaacacaatt
    42541 agcacgtgaa agcttcaatc ctccaacatt aaaaattaac agtgataagt ctattttcga
    42601 cataaattat gaagatttgg aaattgttga ctatgaatca catccagcaa taaaagctcc
    42661 aatagcagtg tagtcattgc atagttagct aactatatag acatcaaaat attttcaagt
    42721 gcaaaaatgt acttttttgt gttaaacgtt ttcataaatt atgcaaaatc attatttcta
    42781 tcacacttta tgataaaaat tgtgttaaat taaagataac ttagtaataa aaaatgaaat
    42841 gatagaagaa ggaggataat tatgacttta tccattctag ttgcacatga cttgcaacga
    42901 gtaattggtt ttgaaaatca attaccttgg cacctaccaa atgatttgaa gcatgttaaa
    42961 aaattatcaa caggtcatac tttagtaatg ggtcgtaaga catttgaatc gattggtaaa
    43021 ccactaccga atcgtcgaaa tgttgtactt acttcagata caagtttcaa cgtagagggc
    43081 gttgatgtaa ttcattctat tgaagatatt taccaactac cgggccatgt tttcatattt
    43141 ggagggcaaa cattatttga agaaatgatt gataaagtgg acgacatgta tattactgtt
    43201 attgaaggta aattccgtgg tgatacgttc tttccacctt atacatttga agactgggaa
    43261 gttgcctctt cagttgaagg taaactagat gaaaaaaata caattccaca tacctttcta
    43321 catttaattc gtaaaaaata agggggaaaa tgaccatgac aaaacagatt atagtgacag
    43381 actcaacatc cgatttatct aaagaatatt tagaagcaaa caacatacat gtaatccctt
    43441 taagtttaac tattgaagga acttcatacg ttgaccaagt agatattaca tcagaagaat
    43501 ttattaatca tattgaaaat gatgaagatg taaagacaag tcaaccagcc ataggtgaat
    43561 ttatatctgc ttatgaagaa ctaggaaaag atggcgctga aatcataagt attcatcttt
    43621 cttcaggatt aagtggtaca tttaacactg cttatcaagc aagtcaaatg gtagatgcta
    43681 atgtaactgt tattgattca aaatctattt cttttggttt agggtatcaa atacaacacc
    43741 tagtagagct tgtaaaagaa ggtgtctcaa cttctgaaat agttaaaaag ttaaatcatt
    43801 taagagaaaa cattaaatta tttgtagtta tagggcaatt gaatcaatta attaaaggtg
    43861 gcagaattag taaaacaaaa ggtttgattg gtaatcttat gaaaatcaaa ccaattggta
    43921 cactagatga tggtcgctta gagcttgtgc acaatgcgag aactcaaaat tctagtatcc
    43981 aatacttgaa aaaggaaatt gctgaattta taggagatca tgaaatcaaa tccgttggtg
    44041 tcgcacatgc taacgtcatt gaatatgttg ataaattgaa gaaagttttt aatgaagctt
    44101 ttcatgtgaa taattacgat ataaatgtaa ctacaccagt tatttctgca catactggtc
    44161 aaggtgcgat tggcctcgta gtccttaaga agtaaattta atcttttcag tgttaattac
    44221 ttccatttca atcctttata gactaaattt ataattagat agatagagga ggtaattcat
    44281 atgacaaaag aatatgcaac attagcagga ggatgtttct ggtgcatggt taaaccattt
    44341 acatcatatc caggcatcaa gtcagtcgta tctggttata gtggcggtca tgttgacaac
    44401 ccaacttatg aacaggtatg tacgaatcaa accggccatg tcgaagcagt acaaattacg
    44461 tttgatccag aggttacttc ctttgaaaat atattagaca tatatttcaa aacatttgac
    44521 ccaactgatg atcaagggca atttttcgat agaggcgaaa gctatcaacc agtcattttc
    44581 tatcatgatg agcatcagaa aaaggctgct gagtttaaaa agcaacaatt aaatgaacaa
    44641 ggtattttca agaaaccagt gattacacct attaaaccat ataaaaattt ctatccagct
    44701 gaagactacc atcaagatta ttataaaaag aacccggtac attattacca atatcaacgt
    44761 ggttcaggta gaaaagcgtt tatagaatca cattggggga atcaaaatgc ttaaaaaaga
    44821 taaaagtgaa ctaacagaca tagaatatat tgttacacaa gaaaatggca ctgaaccacc
    44881 atttatgaat gaatattgga atcattttgc taaaggaatt tatgtagata agatttctgg
    44941 taaaccttta tttacatctg aagaaaagtt tcattctgaa tgtggatggc ctagcttttc
    45001 caaagcgctt gatgacgatg aaattataga attagtcgac aaatcatttg gtatgttgag
    45061 aactgaagtg cgttcagaag aatcaaatag tcatttagga catgtcttta atgatggacc
    45121 taaagaaagt ggcggcttaa gatactgtat caattccgct gcaattcaat ttattccata
    45181 tgaaaaatta gaagaattgg gttatggcga tttaatatca cattttgata agtaggagtg
    45241 gaaagtatgt ttaaaaaatt attcggaaaa ggcaaagaag ttcagaaaga tatcgcaatt
    45301 tatgcaccat taactggaga atacgtgaaa attgaagata ttccagatcc tgtattcgca
    45361 caaaaaatga tgggcgaagg gtttggtatt aatccaactg aaggagaagt tgtgtctcca
    45421 attgcaggac gtgttgataa tgtctttcca actaagcatg cgattggttt aaaagcagat
    45481 aatggattag aattattagt tcatatcggt ttagacacag ttcaattaga cggtgaagga
    45541 tttgaagtgt tagtgtctag tggtgacgaa gttaacgtcg gagatccatt agtaagattc
    45601 aaccttgaat atatcaataa taacgctaaa tctgttattt caccaattat tattactaac
    45661 acagatcaag cggcatcaat taatatttat gatgaaaatg ctgtgattaa aggtgaaaca
    45721 aaagtgattg atgtgacaat gaactaatga aaaactattc gttttatcaa tttgtcatga
    45781 cagttcgtgg gcgacacgat gataaaggtc gtctagcaga agagatattt gacgatcttg
    45841 ctttcccaaa acacgatgat gattttaaca tactgtctga ttatattgag acacatggtg
    45901 atttcacatt gccaatgtct gtatttgatg atttatatga agaatatacg gaatggctaa
    45961 aattttaata taataatagt tagaaccagg gtgatgcaag ttgttatcct ggttttaatt
    46021 taaaataaac taagttgtga ctaaaaatta atcaattata gtgaaatatg gtgcgctatc
    46081 ttgcataaat tgatatgatt aactacacag aatttaaaag tacataatac ataataagga
    46141 agtgatacaa tggatgataa gcaacacaca tcttcatccg atgatgaacg cgctggaatt
    46201 gctacaagca atcaagacca acaaactaat tcgtcgaaac gcgttcactt aaaacgttgg
    46261 caattcatat caattttaat tggtacaatc ctaataacag ctgtcatcac agttgttgca
    46321 tatattttta taaatcaaaa aataagtggt ttaaacaaaa ctgatcaagc aaacttaaat
    46381 aaaattgaaa atgtgtataa aatcttaaat agtgattatt ataaaaaaca gagctctgac
    46441 aagttgagta aagctgcaat tgatggcatg gtcaaagaat taaaagatcc ttattctgaa
    46501 tatttaacaa aagaacaaac gaaatccttt aatgaaggtg tttcaggtga ttttgtaggt
    46561 attggtgcag aaatgcaaaa gaaaaatgat caaattatgg ttactagccc tatgaagggt
    46621 tctccagcag aacgtgctgg catccgtcct aaagatgtca ttactaaagt aaatggaaaa
    46681 tcaattaaag gtaaagcatt agatgaagtt gtcaaagatg ttcgtggtaa agaaaacact
    46741 gaagtaactt taactgttca acgaggcagt gaagaaaaag acgttaagat taaacgtgaa
    46801 aaaattcatg ttaaaagtgt tgagtataag aaaaaaggta aggttggagt tattactatt
    46861 aataaattcc agaatgatac atcaggtgaa ttgaaagatg cagttcttaa agctcacaaa
    46921 gatggtttga aaaagattgt tttagattta agaaataatc caggtggact actagatgaa
    46981 gctgttaaaa tggcaaatat ttttatcgat aaaggaaaaa ctgttgttaa actagaaaaa
    47041 ggtaaagata ctgaagcaat tcaaacttct aatgatgcgt taaaagaagc gaaagacatg
    47101 gatatatcca tcttagtgaa tgaaggttca gctagcgctt ctgaagtgtt tactggtgcg
    47161 ctaaaggact ataataaagc taaagtttat gggtcaaaaa cattcggcaa aggtgtcgta
    47221 caaactacaa gagagtttaa ggatggttca ttgttaaaat atactgaaat gaaatggtta
    47281 acgccagatg gtcattatat tcacggtaaa ggcattaaac cagacgttac tattgacaca
    47341 cctaaatatc aatctttaaa tgtcattcct aatacaaaaa catttaaagt tggagacgat
    47401 gataaaaata ttaaaactat taaaattggt ttatcagctt taggttataa agttgataat
    47461 gaaacaacgc aatttgatca agctttagaa aatcaagtta aagctttcca acaaacgaat
    47521 aaactcgagg taactggtga atttaataaa gaaacgaata ataaatttac tgagttatta
    47581 gttgaaaaag ctaataaaca tgatgatgtt ctcgataa
//
LOCUS       DAHHRR010000007        46805 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.007, whole genome shotgun sequence.
ACCESSION   DAHHRR010000007 DAHHRR010000000
VERSION     DAHHRR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 46805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46805
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.007"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            85..684
                     /locus_tag="NBI42_000297"
     CDS             85..684
                     /locus_tag="NBI42_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HCD1776763.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQVRTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            719..828
                     /locus_tag="NBI42_000298"
                     /pseudo
     CDS             719..828
                     /locus_tag="NBI42_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            908..1027
                     /locus_tag="NBI42_000299"
     CDS             908..1027
                     /locus_tag="NBI42_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776764.1"
                     /translation="MGFCPPVTMDACALLQKFDFYNNISHFRHFFAIKQPIER"
     gene            1354..2580
                     /locus_tag="NBI42_000300"
     CDS             1354..2580
                     /locus_tag="NBI42_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1776765.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWSKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDTIFVVDRDKHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDRQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            2577..3212
                     /locus_tag="NBI42_000301"
     CDS             2577..3212
                     /locus_tag="NBI42_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1776766.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIILTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLILGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGIDALLALVEKWVVPKGLKVSG"
     gene            3229..4170
                     /locus_tag="NBI42_000302"
     CDS             3229..4170
                     /locus_tag="NBI42_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1776767.1"
                     /translation="MKKIKYILVVLVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKDPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            4170..4865
                     /locus_tag="NBI42_000303"
     CDS             4170..4865
                     /locus_tag="NBI42_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1776768.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            5159..6559
                     /locus_tag="NBI42_000304"
     CDS             5159..6559
                     /locus_tag="NBI42_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1776769.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLIAVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEMWYIVSSIVGYLLFGIGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAII
                     AFLVILLLVPKNQTNL"
     gene            6787..7722
                     /locus_tag="NBI42_000305"
     CDS             6787..7722
                     /locus_tag="NBI42_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCD1776770.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYGVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEAMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            8045..8896
                     /locus_tag="NBI42_000306"
     CDS             8045..8896
                     /locus_tag="NBI42_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD1776771.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDEPVKGSEFAEWYKAQLGVEPNIDIQPAQPFERGVSNEKFKAQGGSLIYKTWK
                     DGMNPIK"
     gene            complement(8987..10483)
                     /locus_tag="NBI42_000307"
     CDS             complement(8987..10483)
                     /locus_tag="NBI42_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase FLP"
                     /protein_id="HCD1776772.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQTETITIKQLLAQTSGIPSDITSEDA
                     VTNKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNNKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKNQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGKIILRKSKLTTFLSWLTLCSCIAIALILYALPY
                     LILGSNNWSFVLTWLPIEIKLTLITTLIALFSTLIVILLFLHTKITKT"
     gene            10715..12544
                     /locus_tag="NBI42_000308"
     CDS             10715..12544
                     /locus_tag="NBI42_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCD1776773.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSANNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     MPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWLHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(12683..14761)
                     /locus_tag="NBI42_000309"
     CDS             complement(12683..14761)
                     /locus_tag="NBI42_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCD1776774.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKD"
     gene            complement(14900..16309)
                     /locus_tag="NBI42_000310"
     CDS             complement(14900..16309)
                     /locus_tag="NBI42_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1776775.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            16595..17401
                     /locus_tag="NBI42_000311"
     CDS             16595..17401
                     /locus_tag="NBI42_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCD1776776.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKNLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQRGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVMDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(17464..18120)
                     /locus_tag="NBI42_000312"
     CDS             complement(17464..18120)
                     /locus_tag="NBI42_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCD1776777.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            18233..18706
                     /locus_tag="NBI42_000313"
     CDS             18233..18706
                     /locus_tag="NBI42_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1776778.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GSVPKAVQQKMEEVHHYIHQYQAETVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(18829..20040)
                     /locus_tag="NBI42_000314"
     CDS             complement(18829..20040)
                     /locus_tag="NBI42_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCD1776779.1"
                     /translation="MQSSKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSVMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(20223..20882)
                     /locus_tag="NBI42_000315"
     CDS             complement(20223..20882)
                     /locus_tag="NBI42_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776780.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     EVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(20942..22084)
                     /locus_tag="NBI42_000316"
     CDS             complement(20942..22084)
                     /locus_tag="NBI42_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD1776781.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVFGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDTRLKEVTFKVACDVNNPLLGEHGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            22352..22738
                     /gene="gtxA"
                     /locus_tag="NBI42_000317"
     CDS             22352..22738
                     /gene="gtxA"
                     /locus_tag="NBI42_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HCD1776782.1"
                     /translation="MKLTQTHAEILKFVIVGGINTLNYYVVYLLLLKLFRIEYMISHI
                     TGFIVAFVISYYLNCYFVYRVKPTWRKFISFPITQLVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLVITIPITFVLSKWILKDSN"
     gene            22872..22979
                     /locus_tag="NBI42_000318"
     CDS             22872..22979
                     /locus_tag="NBI42_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD1776783.1"
                     /translation="MFNLLINIMTSAISGCLVAFFAHWLRTRSNKKGDK"
     gene            23166..23333
                     /locus_tag="NBI42_000319"
     CDS             23166..23333
                     /locus_tag="NBI42_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776784.1"
                     /translation="MQLLILTDIFYRIAVYICPNSVRSLSHFNSKVKILPKFIVIRRS
                     LNSCFLEKHAP"
     gene            23388..24074
                     /gene="bioD"
                     /locus_tag="NBI42_000320"
     CDS             23388..24074
                     /gene="bioD"
                     /locus_tag="NBI42_000320"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HCD1776785.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYYAMKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMADQIFLNKQRVLEKVKVLDK
                     EFDIVLIEGAGGIGVPLYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            24052..25410
                     /gene="bioA"
                     /locus_tag="NBI42_000321"
     CDS             24052..25410
                     /gene="bioA"
                     /locus_tag="NBI42_000321"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCD1776786.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSLYRSNYETEAEMMAAILQNIEQILIDRNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCNKYNILLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSQKIYDAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENINLYEKRKLLSQIKKTSTTLEKQLQTLKRHPNIGDVRGRGLMFGVELVADKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            25412..26419
                     /gene="bioB"
                     /locus_tag="NBI42_000322"
     CDS             25412..26419
                     /gene="bioB"
                     /locus_tag="NBI42_000322"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCD1776787.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRA
                     N"
     gene            26397..27512
                     /locus_tag="NBI42_000323"
     CDS             26397..27512
                     /locus_tag="NBI42_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HCD1776788.1"
                     /translation="MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVEKERFVNFTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSVAYEEIESMLSGWLGYSACTIFN
                     NGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLAE
                     CPNQNIQKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGI
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDVDRLFEILHQED"
     gene            27523..28215
                     /locus_tag="NBI42_000324"
     CDS             27523..28215
                     /locus_tag="NBI42_000324"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HCD1776789.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIIEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMRYIENETV
                     YTGAIILSATSGKRLDSFGQRGIRATHFSFEDINCKANLTERVKDALAIASCVNAHSY
                     VKGELCVSDDLTYTTGYFASAKIGYHRLFDIKPVNTRYGGRIIFVDDSIDLDHYISFL
                     ESTPKQVIYETV"
     gene            28226..28693
                     /locus_tag="NBI42_000325"
     CDS             28226..28693
                     /locus_tag="NBI42_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCD1776790.1"
                     /translation="MTSKDMTQISAIAAILTILAVLKIPSILPGLDFQLSAPVALLIL
                     AFFGIKKYFLGGLLSSIILLILGVFNPINVMISLTFRLIAIFVVYLLKINVLSLVLAS
                     VLGSLLSRLLLSIILNLPVWAVLLNTIPGIIFTIILAIPLYLTLRKRMAVLLR"
     gene            complement(28744..29721)
                     /gene="hlgB"
                     /locus_tag="NBI42_000326"
     CDS             complement(28744..29721)
                     /gene="hlgB"
                     /locus_tag="NBI42_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HCD1776791.1"
                     /translation="MKMNKLVKSSVATSMALLLLSNTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKAAGNINSGYEKPNPKDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSSYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHKQNGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETEINK"
     gene            complement(29723..30670)
                     /gene="hlgC"
                     /locus_tag="NBI42_000327"
     CDS             complement(29723..30670)
                     /gene="hlgC"
                     /locus_tag="NBI42_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HCD1776792.1"
                     /translation="MLKNKMLATTLSISLIAPLATPLFENAKAANDTEDIGKGSDVEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTAYYNYKKSNHVKA
                     MRWPFQYNIGLKTNDPNVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKGVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            complement(31237..32166)
                     /gene="hlgA"
                     /locus_tag="NBI42_000328"
     CDS             complement(31237..32166)
                     /gene="hlgA"
                     /locus_tag="NBI42_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HCD1776793.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDPNVDLINYLPKNKIDSADVSQKLGYNIGGNFQTAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGIKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
     gene            complement(32703..34016)
                     /locus_tag="NBI42_000329"
     CDS             complement(32703..34016)
                     /locus_tag="NBI42_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B domain-containing protein"
                     /protein_id="HCD1776794.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKQQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQASKAKERQNIENANKA
                     IQDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKAANDAI
                     TKLQKDDSIENRRLAQREVNKAPMDVKEHLQKQLDTLVAQKDAKKKSSPKVEVPQIQS
                     PQIEKPKAESPKVEVPKIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNKYYQYKGLIDQTVLTTIGSGYGSYIKPLEVSKESGNLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTITGLFNSFWK"
     gene            34111..34215
                     /locus_tag="NBI42_000330"
     CDS             34111..34215
                     /locus_tag="NBI42_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776795.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            34714..35580
                     /locus_tag="NBI42_000331"
     CDS             34714..35580
                     /locus_tag="NBI42_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD1776796.1"
                     /translation="MSHNENLKLAQHGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            35908..36594
                     /locus_tag="NBI42_000332"
     CDS             35908..36594
                     /locus_tag="NBI42_000332"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCD1776797.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(36748..36852)
                     /locus_tag="NBI42_000333"
     CDS             complement(36748..36852)
                     /locus_tag="NBI42_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCD1776798.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            37026..38465
                     /gene="mdeA"
                     /locus_tag="NBI42_000334"
     CDS             37026..38465
                     /gene="mdeA"
                     /locus_tag="NBI42_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HCD1776799.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYERFGPRKLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            38586..39365
                     /locus_tag="NBI42_000335"
     CDS             38586..39365
                     /locus_tag="NBI42_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCD1776800.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            39346..40065
                     /locus_tag="NBI42_000336"
     CDS             39346..40065
                     /locus_tag="NBI42_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD1776801.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            40062..40793
                     /locus_tag="NBI42_000337"
     CDS             40062..40793
                     /locus_tag="NBI42_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD1776802.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GTPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(40890..42140)
                     /gene="fmhA"
                     /locus_tag="NBI42_000338"
     CDS             complement(40890..42140)
                     /gene="fmhA"
                     /locus_tag="NBI42_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HCD1776803.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGEVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKQNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDTLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLKEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            42395..42757
                     /locus_tag="NBI42_000339"
     CDS             42395..42757
                     /locus_tag="NBI42_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCD1776804.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQDFDS
                     RRYYQQHDADYHEENMTN"
     gene            42776..43375
                     /locus_tag="NBI42_000340"
     CDS             42776..43375
                     /locus_tag="NBI42_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCD1776805.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKSGKPLIVIYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            43382..43482
                     /locus_tag="NBI42_000341"
                     /pseudo
     CDS             43382..43482
                     /locus_tag="NBI42_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            43647..43898
                     /locus_tag="NBI42_000342"
     CDS             43647..43898
                     /locus_tag="NBI42_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD1776806.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            43898..44164
                     /locus_tag="NBI42_000343"
     CDS             43898..44164
                     /locus_tag="NBI42_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCD1776807.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALK
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            44458..46005
                     /gene="adcA"
                     /locus_tag="NBI42_000344"
     CDS             44458..46005
                     /gene="adcA"
                     /locus_tag="NBI42_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HCD1776808.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHGGYDPHVWLDPKI
                     NQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHE
                     SIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETI
                     RKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESK
                     HDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDL
                     KAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFKL
                     VDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQI
                     KEEMLAH"
     gene            46191..46658
                     /locus_tag="NBI42_000345"
     CDS             46191..46658
                     /locus_tag="NBI42_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCD1776809.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSEFNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
BASE COUNT        15780 a         6629 c         8658 g        15738 t
ORIGIN      
        1 caaaagcatt tgtaaaatta gttttttata aaattaggat atataataca atgtgtattg
       61 aaaaataaaa tggaagtgat gactatgagt gagaaacaat cgaatgaaca agttgaggca
      121 tttttaaata aagagagtca atggcaagaa gaatataaat atttgagagc gttaatcttt
      181 aatgaaacag aattagagga agcgtataaa tggatgcatc cttgttacac gttgaataat
      241 aaaaatgtag tacttatcca tggcttcaaa aattatgttg cactattatt tcataaaggc
      301 gccattttgg aggataaata tcatacactc attcaacaga ctgaaaaggt gcaagcagct
      361 cgtcagttac gatttgaaaa tttaacagag attcaagtac gtaccgaaga aattaaatat
      421 tatctagctg aagcaattaa agctgaaaaa gctggtaaaa aagttgaaat gaagaaaact
      481 gaggaatatg ttattcctaa agagttagaa gctaagttcg aagaaatgcc acagctagag
      541 tcgtcatttt ataaattaac gccaggcaga caacatcaat atatatatca tattggacaa
      601 gctaaacgca gtgaaacaag acaaaagcgt gttgaaaagt atattaacca aatactagaa
      661 ggtaaaggga tgcatgataa gtaattaatg agtaaaagca taccagttat acaacaacat
      721 acaagatgac acgaaacaac caatggctca tgctgttggt tgttttttag gtgtgcctgt
      781 catgggcaac actttgacgg tggaattccg ttacagactt gggagtagga actgttagca
      841 aaaggcaagg gtgtctacaa tgaatgatga agatattaaa atataaggat gactttgtgg
      901 gtggcggatg ggcttttgtc cgcctgtaac aatggatgcg tgtgcattat tacaaaaatt
      961 cgacttttac aataatattt cacatttccg acactttttt gctataaaac aaccaattga
     1021 gcgataataa attcgctttt gaaaaatatg agttatctat ttagttgcaa aagataaaat
     1081 gataatgttt aataacatca tatagagtat gttagtttta aatgtcgaat atacgaatgt
     1141 gcaaacaaag taatcggtag aaattcaaca tacatagcgc cgtttactgt taagtattca
     1201 cattacagat gaaaaatata aaattctaca taatcaagac catgatgtgt acttgtttaa
     1261 cttatgactc tatttgttta acaattgcga tgatggtctt tttattttat gcgtatcatt
     1321 cgtcatattt tttatgagga aggagaaatg attatgttaa gtattaagca tttaacgaaa
     1381 atttattctg gtaataaaaa ggcagtagat gacatctctt tagatattca atctggggaa
     1441 tttatcgcat ttattggaac cagtggaagt ggcaaaacga ctgctttaag aatgataaat
     1501 cgtatgattg aagcgacaga aggacaaatt gaaattgatg gtaaagacgt acggagtatg
     1561 aatcctgtcg aattacgtag aaatattggc tatgttattc aacaaattgg cttaatgcct
     1621 catatgacga ttaaagagaa tattgtgttg gtacccaaat tgttgaaatg gtctaaagaa
     1681 gaaaaggata aacgtgcaaa ggaattaatt aaacttgtgg atttaccgga gtcattttta
     1741 gaacgttatc cagcagaact atcaggtggg caacaacaac gtatcggtgt cgtaagagca
     1801 cttgcggccg aacaagatat tattttaatg gatgaacctt ttggtgcatt ggatcctatt
     1861 acgagagata cgttacaaga tttagttaaa acgttacaac gaaaattagg caagacgttt
     1921 atctttgtaa cacatgatat ggatgaagcg attaaattag cagacaaaat ttgtattatg
     1981 tcagaaggta aggtggtgca atttgataca ccagacaata ttttaagaca tcccgcaaat
     2041 gattttgtac gtgattttat aggacaaaat agactgattc aagaccgtcc caatgacaag
     2101 actgtagaag gtgtaatgat taaaccaatc acgatacaag cagaagcaac actaaatgac
     2161 gccgttcata ttatgagaca aaaacgtgtt gatactattt ttgtagtaga tagggataag
     2221 catttactag gatttttaga tattgaagat ataaatcagg gtatacgtgg acacaaaagt
     2281 ttacgagata ccatgcaaca acatatttat accgttcaaa ttgattctaa gttacaagat
     2341 tctgtacgta cgattttaaa aagaaacgtt aggaatgtac ctgtcgtaga tgataggcaa
     2401 cgtttagtag gactgattac gcgtgccaat gttgttgata ttgtatatga cacgatttgg
     2461 ggcgatagtg aggatacagt gcaaacagaa catgtggggg aagacactgc gtcctcaaaa
     2521 gtgcatgagc aacacactac taatgtcaaa gtacgtgata taggagatga taaatcatga
     2581 tcgagttcct acatgaacat ggtggacagt tgatgtcgaa aacactggaa catttctata
     2641 tttctatagt ggcattatta cttgccatca ttgttgcagt acctataggc attttattat
     2701 caaaaacaaa gcgaactgcc aatattatat taactgtggc aggtgtctta caaactattc
     2761 caacactagc tgtacttgct ataatgatac cgatttttgg tgttggtaaa acgcctgcaa
     2821 ttgtagcgct atttatttat gtattattac ctattttaaa taacacggta ctcggtgttc
     2881 aaaatattga tagcaacatt aaagaagctg gaaaaagtat gggaatgacg caatttcaat
     2941 tgatgaagga tgtggaatta ccattagcct taccgcttat actaggcggt attcgcttgt
     3001 cgtctgtgta tgtaattagt tgggctacac ttgcaagtta tgtaggtgcg ggtggattag
     3061 gtgatttcat tttcaatggt ttaaatttat atgatccact gatgattgta actgcaacgg
     3121 tactcgttac tgcactagca ttaggtattg atgccttatt agctttagtt gaaaaatggg
     3181 tagttcccaa aggcttaaaa gtatctggat aattaggagg ctaagataat gaagaaaatt
     3241 aaatatatac ttgtcgtgct tgtcttatcg cttaccgtat tatctggatg tagtttgccc
     3301 ggactaggta gtaagagcac gaaaaatgat gtcaaaatta cagcattatc aacaagcgaa
     3361 tcgcaaatta tttcacatat gttacggttg ttaatagagc atgatacaca tggcaagata
     3421 aagccaacat tagtaaataa tttagggtca agtacgattc aacacaatgc cttaattaat
     3481 ggggatgcta atatatcggg tgttagatat aatggtacag atttaacggg agctttgaag
     3541 gaagcaccaa ttaaagatcc taagaaagca atgatagcca cacaacaagg atttaaaaag
     3601 aaatttgatc aaacgttttt tgattcgtat ggttttgcga atacgtatgc attcatggta
     3661 acgaaggaaa ctgctaaaaa atatcattta gagacagttt cagatttagc aaagcatagt
     3721 aaagatttac gtttaggtat ggatagttca tggatgaatc gtaaaggcga tggctatgaa
     3781 gggtttaaaa aagagtatgg tttcgacttt ggtacagtga gaccaatgca aataggtcta
     3841 gtctacgacg cattaaactc agagaagtta gacgttgcat taggttactc tacagatggt
     3901 cgaattgccg cgtatgattt gaaagtactt aaagatgata aacaattttt cccaccttat
     3961 gctgcgagtg ctgttgcaac aaatgaatta ttacgtcaac atccagaact taaaacgacg
     4021 attaataagt tgactggaaa gatttcaact tcagagatgc agcgcttgaa ttatgaagcg
     4081 gatggtaaag gtaaagaacc tgctgtcgtc gcagaagaat ttttgaaaaa acaccactat
     4141 tttgataaac agaaaggtgg tcataagtaa tggaaggtaa tttattacag caattattca
     4201 attattatgt tacgaacttt ggctatctat gggatttatt ttttaaacac ttattaatgt
     4261 ctgtctatgg tgtgctattt gcagctttaa ttggtattcc attgggaatc ttgcttgcaa
     4321 gatacacaaa actttctgga tttgtaatta caattgcaaa tataattcaa acagttccag
     4381 tcattgcaat gttagctatt ttaatgttag tcatgggctt aggctcagaa acagtagttt
     4441 taacagtgtt tttatatgcg ttacttccaa ttataaaaaa cacttatact ggtatagcta
     4501 gtgttgatgc gaatattaag gatgctggca aaggtatggg aatgacacgc aatcaagtgc
     4561 tacgaatgat tgaattaccg ttatctgttt cggttattat cggtggcatt cgtattgcct
     4621 tggttgttgc gataggtgtt gttgccgttg gatcatttat aggagcacct acgcttggtg
     4681 acattgtgat tcgtggtaca aatgcgacgg atggcacaac gtttatttta gcaggtgcga
     4741 ttccgattgc tatcattgca atcgtcattg atgtactatt aagattttta gaaaaacgat
     4801 tagacccaac gacacgacat cgtaaaaatc aatctaatca tcgtccgcaa agtattaata
     4861 tgtaatagta gaagatgttt ataatttagc gattccgttt catgatttat aaaaaatgag
     4921 gctactcaag gagctcaaat aatctttgag tagccttttt ataggttgta tttgtatgcg
     4981 tttacactaa aatagcaatt attatcatga aagtttttgg ataaaaagcg ttaattattg
     5041 taaaaatact aaataatgag atgttttatt tataattttc tgcaaattta tgatattgtt
     5101 tcttaatata tcatattaaa aatttgtttt tcttaaacat aggaggctta tctaattcat
     5161 ggacacatca aaacaattta gaggtgacaa ccgattgctt ttgggtatcg ttttaggggt
     5221 tattaccttt tggctattcg cgcagtcact tgttaatctt gttgtcccat tacaatcaac
     5281 atatagtagt gacgttggaa cgataaatat cgctgttagc ttatctgcct tatttgctgg
     5341 tttgtttatc gtaggtgctg gtgatgttgc tgataaattt ggtcgcgtca aaattactta
     5401 tgtaggattg atattaaatg ttgtaggttc attactcatc attattacac ctttgccagc
     5461 atttttaatt ataggtagaa taattcaagg tttgtctgca gcatgtatta tgccatccac
     5521 acttgctatt attaacgaat attatattgg tacaagaaga caacgtgcct taagctattg
     5581 gtctattggt tcttggggtg gtagtggtat ttgtacgttg tttggtggct taatggctac
     5641 atatataggt tggcgttcaa tatttgttgt ttcaattcta ttaacattat tagcaatgta
     5701 cttaatcaaa cacgcacctg agactaaagc agaaccaatc aaaggtatga aagcagaagc
     5761 taaaaagttt gacgttatcg ggttagtcat tttagtagtg acaatgttaa gtttaaatgt
     5821 aatcatcaca cagacgtctc attttggttt agtttcaccg ttaattctag gtttaattgt
     5881 agtgtttatc tgttcattag ttggttttgt atattacgaa aataaaataa agcatccact
     5941 tgttgatttt tcaattttta aaaatagagg atacagtggt gcaactattt caaacttctt
     6001 attaaatggt gtagcaggtg gtgcacttat cgtcattaac acgtattatc aacaacaatt
     6061 aggatttaat tcttcgcaaa cgggttatat ttcattaacg tatttaatag cagtgttgtc
     6121 aatgattcgt gtaggtgaaa agattttatc tcaacatggt ccgaaacgcc cactattact
     6181 aggaagtggc tttacagtga ttgggttaat cttattgtcg ttaacatttt taccagaaat
     6241 gtggtatatc gtatctagta tagttggata tttattattt ggtattggtt taggattata
     6301 tgctacacca tcaactgata cagcagttgc tagtgcgcca gatgataagt cgggtgttgc
     6361 ttcaggcgta tataaaatgg cgtcatcatt aggaaatgca tttggagtgg cagtatctgg
     6421 tacggtttat actgtgttag cagctaattt aaatttgaac ttaggtggtt tcacaggtat
     6481 gatgtttaat gccttgctag caattattgc atttttagtc attttactat tagttcctaa
     6541 aaatcaaacg aatttgtaaa actgaaatga aagcaagtta acatgtacgg attttaaagg
     6601 aaattttgtg gaagtaagta tatcatacac acttaatgtt gcgtattgac gtttaatgtt
     6661 aggtgtgttc ttttatagac gataaaagct gtgtgtgtat attaagcgaa tgattttcaa
     6721 attgacgcta atatgcgaaa gtagtatttt aaaaatgaac aacaacgatg aagaggggtt
     6781 tatagaatga aaattgcaat tgctggatcg ggtgcattag gtagtggctt tggtgccaaa
     6841 ctatttcaag caggatatgg tgtcacactt attgacggat atacatctca tgttgaagcg
     6901 gttaagcaac atggattaaa tataacgatt aatggagagg cattcgagtt aaacattccg
     6961 atgtatcatt ttaatgatca accggacgaa agcatttacg atgtggtctt tctatttcca
     7021 aagtctatgc aattaaaaga agtgatggaa gctatgaagc cacatattga taatgaaacg
     7081 attgtcgtat gtacgatgaa tggtttgaag catgaagaag tcattgcgca gtatgttgct
     7141 caatcacaaa ttgtcagagg tgttacgact tggacggcag gtcttgaaag ccctggacac
     7201 agtcatttac ttggtagtgg accagttgaa ataggtgaac tagttgatga aggtaaagaa
     7261 aatgttataa aagttgctga tttacttaac gaagcggaat tgaatggtgt cattagtaaa
     7321 gatttatacc aatcgatttg gaaaaagatt tgtgttaatg gtacggcaaa tgcattaagc
     7381 acagtgttgg agtgtaatat ggcatcactg aatgaaagta gttatgcgaa gtgtttgatt
     7441 tataaattaa cgcaagaaat agtgcatgta gcgacgattg ataatgttca tttaaatgtt
     7501 gatgaagtat ttgaatattt agttgattta aatgaaaaag ttggtgcgca ttatccatcc
     7561 atgtatcaag atttaattgt taataataga aaaactgaaa ttgattatat taatggcgca
     7621 gttgcaacat taggtaaaca acgtcatatt gaagcgccag tcaatcgctt tattactgat
     7681 ttaattcata ctaaagaaag tcagcgacat gcacaggatt aattagtgtt tttaggtatg
     7741 attgcaagga aatgacaacc acgtgataat tacggtcatt attaaaattg aaatgtaata
     7801 aataaagaac agcagtaagg tactttcaaa ttgaaatgat cttggtgctg tttttcttga
     7861 ttgatcttcg tcataattca gatttgtcat aggtctacga catactacta gcatttgtta
     7921 gacagttttt acgacgacac tttgaaaaat tttgaggcaa atcatttgga agtctcacgt
     7981 gaattttgta cactcatcaa gcaaataatt atattaaaag acaaatagag aaaaggtgtt
     8041 tataatgagt aaaatttttg taacaggtgc aacgggcctt attggcatta aattagttca
     8101 aagactaaaa gaagaggggc atgaggttgc tggttttact acatctgaga atggtcaaca
     8161 aaagctagct gctgttaatg taaaagcata tattggtgat atattaaaag ctgatactat
     8221 tgatcaagcg ttagcagatt ttaaaccaga aatcattatc aaccaaatta cggatttaaa
     8281 aaatgttgat atggcagcca atacgaaagt acgtattgaa ggttctaaaa acttaattga
     8341 tgcggcgaaa aagcatgacg ttaagaaagt aattgcccaa agtattgcct ttatgtatga
     8401 acctggcgaa ggattagcaa atgaggaaac ttcacttgat tttaactcaa ctggtgatag
     8461 aaaagtaacg gttgatggtg tggttggtct agaagaagaa acggctcgta tggatgaata
     8521 cgttgtttta cgttttggct ggttatatgg cccaggtact tggtacggaa aagatggcat
     8581 gatttataat caatttatgg atggtcaagt gacactttca gatggcgtaa catcatttgt
     8641 gcatcttgat gatgcagttg aaacatctat tcaagctatt cattttgaaa atggtattta
     8701 caatgttgct gatgatgaac ctgtaaaagg ttctgaattt gcagaatggt ataaagcaca
     8761 actaggtgtt gagccaaata ttgatattca acctgcacaa ccatttgaac gtggtgtgag
     8821 taatgagaag ttcaaagcac aaggtggctc tttaatttat aaaacatgga aagacggcat
     8881 gaatccaatt aaataataat ttatccgttt aatatgtaaa aaataaagac ttggtcgaat
     8941 cgtggatgat atattatcaa acgcacggct cgaacaagtc tttttattat gtcttcgtta
     9001 tctttgtatg aaggaataac agaatgacaa ttaatgtact gaataatgca attaatgttg
     9061 tgattagtgt taatttaatt tctattggca gccaagtcag tacaaaagac cagttattgc
     9121 taccgaggat gagatatggt aatgcatata atatgagtgc tatagcgata catgaacata
     9181 aagttagcca acttaaaaat gtggtaagtt ttgatttgcg caggatgatt ttcccgtgac
     9241 gcatttgcca agcacgataa gctgagaata taaatgctaa taaaataagt gttgtcatca
     9301 aaacggttac aatattaaat tgatttttat attgattgag catagacgca actgtcgaat
     9361 atcgcttgtg atttacaatt tgtgtgttaa gatgctcaac taatttgggt acatattccg
     9421 aatttagatt tgcaagcaca acaattccat aattttgttt tggatttagt aaaataaatg
     9481 atgaaaagtt atcgagcgtt cctgaatgaa acactaaatg ttcatcatta ttggtaaacc
     9541 agccggaagc atatgcattg gcattaggtt caccaattgt tgatgataaa tttttatgtg
     9601 attgttgaac taatgatttg tatttatcag gtggattaag ttggaatttt atccaatgtt
     9661 ccaaatcttc agttgatgtc atcatatatg ctgatggtgt atcccaaagg ttaaattcag
     9721 gtttagagac gacaggtgtc gaaccttgta attcgtagcc aatagcatca tgttttgatt
     9781 tattattagt ttgtttgaat gatgtatgtg tcatatgcaa aggcttgagc catgaatttg
     9841 taatatattt tgtataggat tgcttcgtaa cgttttggat aattaaacct aataaatcat
     9901 agttcatatt tgagtattca aattcttctc cgggcttatg atgtaattca tcacccataa
     9961 ttgcacgggt tacatcattt aaacgattat ttttgtttgt tacggcgtct tcgcttgtaa
    10021 tatcactagg tataccactt gtttgagcca aaagttgctt aatcgtaata gtttcagttt
    10081 gaccattata gttcatttta aaatgaggca catgtttgga tacagcatca tttaagttta
    10141 atcgaccttc ttgagctaat tttaaaattg caagacctgt gaaagctttc gtattagaag
    10201 caatttcata ctttgttgtg ggagaagctt tgactttttt atcaacatct tgataaccat
    10261 aacctttatt taaaaaaact ttgccatttt ttactattaa aattgatgct ccaggaatat
    10321 gtcccttttg taaatcatgt tcgataattg tatctatttg ttgttgcgaa tcattggtta
    10381 accgtgtctt cgtattgcta tttaatgtta tatatatagc aattgaaatg gcgactaaaa
    10441 tgataataga aatggataga aaatacagtt ttttagtagt cataaacgtt cctttttata
    10501 tgttaaattt acttaagtgc ctaatgagat ttattttatt ataccagttc tattgaataa
    10561 attaattaaa gaggtatatt taccattatt tttgacacct tatatgttaa taggtgtata
    10621 attacataaa aaatattaat taactctcta tactttttgg aaagataggc gtatagtgat
    10681 atccatcaaa aagtataaaa aggaatagga gattatgttc aatcaattta aaagacttat
    10741 tatagggcaa cctaaaaaaa acagagaact aaaagacgaa aagatatcga agtttaaagg
    10801 gttagcgatt ctttcgtcgg atgcattatc ttcagtggct tacggtcccg aacaaatact
    10861 gattacatta tcagtagtgg gtgcagttgc aacttggtat actttaccga ttgcaggtgc
    10921 tgttctgatc ttattagctg ctttaattat gtcttatagg caaattattt atgcatatcc
    10981 taaaggtggc ggcgcgtata tggtatccaa gaccaattta ggagaaaaat ggggattgct
    11041 tgcaggaggc tcattattag tcgattacat actgactgtt gctgttagta tatcatctgg
    11101 tgctgatgca tttgttgctg cattcccaag tttatatggg cataaagtac tgattgcatg
    11161 tttattggta ctttttattt taatattaaa tttacgtggt ttaactgaat ctgccaccgt
    11221 attatcctat ccagtatatc tcttcattat agggttagtg atattaatat ttataggtac
    11281 tttccgtgtg gcgacaggag atattcaacc acatatgcat gcatcagtag gaactgcggt
    11341 tcctggagta acattattct tgctattaaa agcattttca tcaggggctt catcattaac
    11401 aggtgttgaa gctatttcaa atgcggtaac caattttaga gaaccaagtg ctaataatgc
    11461 tgtaaaaacg ttaattgcta tgggttcaat tttagcgttt ttattagttg gtattgttgg
    11521 tttagcatat gtttatggaa ttatgccaca aacggaaacg acagttttat cacaattagc
    11581 tatgcaaata tttggtgata atgctgcctt ctattttgta caggcaacga ccgtgatgat
    11641 tttggtatta gcagccaaca ctggatttac agcttttccg atgttagcgg cttcaatgtc
    11701 taaagataaa tatatgccta gaatgtttac tgttagaggt gatcgcttag gctactctaa
    11761 ctcaattatt attttaggtg tattagccat tattttaatt attgtatttg atggtatgac
    11821 tgaagattta attccattgt atgctgttgg tgtatttatt ccatttacac tagcacaatt
    11881 cggtatggtt attaaatggc ttcatgaacg tcctaagaat tggttaagta agttgtctgt
    11941 gaacttactt ggtggtatcg tgacatttat tgtttttatg attttactca taactaaatt
    12001 tagccaagta tggccgatac ttattttctt gccgttcgtg gtgattttct tcttgaaaat
    12061 taacaaacat tatcgagata tcgcagaaca attacgttct gacattgatg ttcttaatgt
    12121 ggacgtggta gatcgtaatt tagcaattgt gccgataact agtattacaa cagcagttga
    12181 taagtctatt tattacgcac aaatgcttgc gaataatgat gtcattgcgg tacatgtatc
    12241 atttggagat gaagatgaaa aagcattcca agagaaatgg aaacgtcatt ttccagatgt
    12301 aagattagtt attttacact cggaatatcg cagtattata cgaccaattt cacgttttat
    12361 cgataagatt aatcgtaaag ccaacgatca aaactatatg attacagtag tgataccaga
    12421 atttattact aagaaacgtt ggcataattt attacacaat caaactagtt tgcgtatgaa
    12481 attgtattta atttatcaaa agaatgttaa cgtatgtaca attccattta aacttaaaaa
    12541 atagattcta taatcaaatc ggattgatga aaggttatta cttttcatca atccgatttt
    12601 ttgatgttga atataaaacc cccgtacata tgagatgata atgactttgt ctcatatgta
    12661 caggggtttt gttatgtctt gcttaatctt tggactgtaa agacaagaca atttcgttgt
    12721 aattgatatt ttctcgaatt tgagctgcaa cgatattgtt gattttattt ttgcgaatta
    12781 attcatctaa aagatgacgt tgtaagtaca atgcttccaa tttgtggttg tggaacatag
    12841 cttgcggtat gatttgattg ctattttctt gacgttcttt acgacgttgt gcacgccact
    12901 ttaatgagcg actcatatca tagtaatgat taatgactaa gctaacctca agtacatttg
    12961 tactattttg ttcttctttt aagcgtaaaa taatattgtg attcacaata cgcataattt
    13021 tttgaacatc tttgaatgaa ttttcaaatt cttgcttctt agattctttg tttaaggctt
    13081 tgtagtgctt catcgcttta agtctaagcc ataacatctt gaagaagttt gcaaattttt
    13141 caacaaatga agcagtacgg tgtgtctctg ttaattcgac aatattgcgg taattattaa
    13201 tatcctgata tgttgcctta cctttatcaa ttaaacgctc aagtgtagat gtttcgatta
    13261 cttttgcaat atcttctaaa cgtttgagtt cttttgtatt ttgattatca ggttctgaat
    13321 ttaataaaaa taacaattct ccatagtatt ggtttaatac tgggcgataa tttgtatcgt
    13381 ttttgtcttt tttactttcg tttttaaaat gctggatcac tttttgaaca atgaaaattt
    13441 tggcagattg ataagtcata cctttaaaag tagtatcttc ggcagatggt gtaattaaag
    13501 gtaaaacaat ttgcgctaag attaaactaa ttaataccat gaaagatgca ataaacaata
    13561 aatcattacg gtattcgaat gcttgtcctt ttgtaatgat aaatggtaat gtaagtgcca
    13621 ttgaaagtga aattgtaccg tgaataccac acatggtcat aataaatgcg taacgcacac
    13681 gagaaggtgg tgtttcatgt gaatcatgtt cctcgtctag ataagattgt atatttttcg
    13741 ggaaataaaa atctttgtac cagaaataaa cccaaacaaa tcgacatgca taaattgcaa
    13801 tagcgattaa tatagtgatc actattaaaa atgaaaagtt gtcaggctct gtttggaaaa
    13861 tatctatgac tacagtagga atcataaaac ctaatacaac aaatacaaaa ccatttaagg
    13921 cataactaaa tgtgttccaa atttgatggt aattcatttg tagttccgtt tgtgctctaa
    13981 ttaaacggtc gcgttctaaa ccatggatta gacctgcgat tacaactgca atgatacctg
    14041 aagcatgaac ttcttctgct aaaaagtata cgacaaaagg agttaataat tgaatgaaag
    14101 ttaaggtatt gttatctttt aaacctttat tagctgttaa gtctatacgt attctaacga
    14161 caacgaatcc aataattgca ccaataagta cacctagtat tgttgaaatg ataaattgtt
    14221 caacagcttg gaataatgaa aaggtaccag ttactaatgc agtaacagca attttaaatg
    14281 aaatgatacc tgctgcatca ttgagtaaag attcaccttc taaaatcgtc atagaacctt
    14341 taggtaataa ttttccgcgt gtaatagcag atactgctac tgcatcagta ggacataaaa
    14401 ttgctgctat tgcaaaagcg gctggcattg gtaaggcagg ccaaatccaa tgtataaaat
    14461 agcctacacc gactacagtt gcaaacacta gtgccattga cattaatagt ataggtttac
    14521 gatattctaa taattttgtt cgagagacgt gggtaccttc cacaaaaagt agtggcgcga
    14581 taacggcaaa cataaatact tcagaattga attggaaatc aacttgtatt ggaataatga
    14641 aaataacgac acctaatgca atttgaataa aggcagtagg aatttgtggg aatcgattat
    14701 tgataaccga actaataatc acagcaaaaa taaaaattaa aaatgcttct aatagtgcca
    14761 tacaatactc ctcaaaattt taatagttaa tattttatca cttttaaggc ataatgacat
    14821 agatatattg ataaaatgaa gttattttca aaaaaaactc tagtatcggt tgaactgata
    14881 ctagagcgag atgtttaaat tattgattgt catatctgaa atgaccgctg tcattttgtc
    14941 gttgttcata cgcgagcttt tcagcattcg ttttgtattt tttataaaag aaaaataaaa
    15001 atataaacca gaatggcgaa atataaatag ctgctcttgt ttcgtcacta aagaataata
    15061 aaatgaatac aaagaagaag aacgctagaa taatgtaagc aataggcttg ccaccaatca
    15121 acttaaattt actgttttta tgtgcctcag gatgcttttt caaatacatt agataagcga
    15181 caataatcat tgcccaaaca actaaaaaca acacagttga taacgttgta acgtatatga
    15241 atagttgaat tgcattaggg aaaatgtagt ttaacaaggc tgctataagt aatgcaattg
    15301 atgaaactaa cattgataaa taaggcacgc catgactatt cgttttattt aaaactttag
    15361 gacctaaccc ttgttgtgac agtccgaata agatacgact attcgaatag ataccactat
    15421 ttgtagcaga ggccgcggca gttagcacga caaagttaac gacacctgct gcaaatggca
    15481 cgccgattaa tgtaaagagt ttgacgaaag ggctactatt tggatcgata tcattccaag
    15541 gtatgactga cataattact aatagaccac cgatatagaa taataaaata cgtataggta
    15601 cattattaat tgctttcggt aaggtttttt caggatcttt cgtttcacca gcagttacac
    15661 ctataagttc aataccaatg aatgaatata cagcaatttg gaatgacatt aagaaaccaa
    15721 atgttcctcc agggaacatg ccgccgtgac tgattaagtt tgtgaatgat gcatgtccat
    15781 aatgtgtttt aaatgagaaa aagattaata caagaccaac gataaccatc gcaataattg
    15841 tgacaacttt aataatcgag aaccaaaact ccagttcacc aaataatctg gcacctaata
    15901 agttgaagct aatcaagatt aaaacaataa atagcacggt aatccaattt gggacttgtg
    15961 gataccaaaa tgcaaagtat tgtcccatcg cagttaggtc tgacatactt gatacaatcc
    16021 aacataacca gtaagtccag ccaatgacaa agccaccaaa tggtcccaaa tattcatttg
    16081 caatatcaac aaatgaatta aatcttgtat tgctcaacaa caattcgcct aatgcacgca
    16141 taaaagcgaa tagtacaacc ccaataatca tgtatgtgaa taacagtgat ggaccagtta
    16201 aagatattgt ttgacctgat cctaggaata acccagttcc aattgcgccc ccaattgcta
    16261 tcaattggat gtgtctgtta ttcaactctc tatgcaattt tcttgccata attttcctcc
    16321 tcaggataaa gcaaataaaa aagttgtgtg taagcgtttt cttaattata aatactaaag
    16381 aaatccattt aataaagcaa ttattttttt aaatataaga aaaaacattt gataggtaag
    16441 gttatgataa ctaaaactta tcgatttttt gaaaagatat aagtttttat gcaaaaatat
    16501 aaaataatca tctaaatgaa gcagttaact atatatatta gataagctat ggcataataa
    16561 ataacttttt aatttgagta aagaaggtat gggtatgaag atagggacaa tcgccgattt
    16621 acatattgat cgtcataata aaaaaacatc cgaagattac ttagaggcat tggttgaaat
    16681 agttaagtat aaaaatttgg atatattgtt gattgcgggg gatatctcaa atcattatca
    16741 attgacgcac caatttataa cgcaattgac aaaacaatta gatatacctg tgaaatttgt
    16801 tcctggaaac catgatttat gggaagttga aagtatgact acgcaagaca tttggaataa
    16861 ttataagagt atgtcacagt gcttggtagg aaaaccattt atagtaaatg aagaatgggc
    16921 aataatagga catacaggtt ggtatgatta tagctttgca gcacaacgat tttcattaga
    16981 tgagttacaa agaggaaaac attatggtgc gacttggcaa gataaagaac gaatatcttg
    17041 gggcatatca gatcaaaatt tatctaaaat agcggctgaa caagtgaaga aagatatatt
    17101 agaagtagga aatagacgag tgattttagt cacacatgtt gtgacgcacc ctgatttcat
    17161 tgttcctatg ccgcatcgta tattcgattt ttataatgca tttattggga caagtgattt
    17221 caatccttta tatgcgatgt tcgatatacc atatagtatt atgggccatg ttcattttcg
    17281 gaaaagtgtg atggatgatg gcagatgtta tctctgtccg tgtctaggct atccaagaca
    17341 atggcgttca gaagatattt accaggaaat aaatgagacg atacaaataa tagaaattta
    17401 aaatgcgcaa acttgacaca gtttgcgcat tttatgtttt acacacgcga gtaatgtgtt
    17461 tacttacgtg tgtttatttt gttgttgatt ttcaattgta tatgaatgtg gttgcacata
    17521 aatgcacttt cttcctggtg aattaaagct gtattccatt ttctctttac gaattttaat
    17581 aatttgtttg cgatttggaa tgatggcagg taaaactagg ccacgacgaa tatgactcca
    17641 aagtggatcg atataatagt aatagtaatc atcgtaccca atgagtaacg ttacgtgaat
    17701 atttgaaact tgtttcgctg gcttgttatc aaagcggaaa acacgtagta atggtttaga
    17761 accaagatta gtatggtata ttaacacagg ttgaccttga tcgataatac ctttaagatc
    17821 ttctaaggat ttaccagtgc cgtctacgat attaggattg tatttttgta aaaatggtac
    17881 atatgcttct ggaaatatcg tttgatgata attgccaagc ttaatgaata agtgatgtcc
    17941 aacataacct ttatgtggat tgttcggatg tgtcggccaa tgtctcataa tttctgtagc
    18001 agggatatgt tggttattgt attgcaacat catggctgcg gaaacacctt cacaccccat
    18061 gaccataggg ataggaaata gctgactgat aggtttaact ggtaatattt ttcggttcat
    18121 aatatagtcc tcgcattgat tcaataaata tttaatataa ttatatagcg tcaatgcaaa
    18181 atgtcctaaa catatgtttt acatgagtga ataaaattaa tggagtgatt aaatggaata
    18241 tcaattacaa caacttgcgt cgttaacgtt agtaggtatt aaagaaacgt atgaaaatgg
    18301 acgacaggct cagcaacata tagcagggtt ttggcaaaga tgttatcaag agggagtaat
    18361 tgcggattta cagttaaaaa ataatggtga tttagccggg atacttggct tatgtatacc
    18421 cgaattagac ggtaagatgt catatatgat tgcagttacc ggagataata gtgctgatat
    18481 tgaaaaatat gatgtcataa cattagcaag ttcaaagtat atggtatttg aagcacaggg
    18541 ctcagtacct aaagcagttc aacaaaaaat ggaagaggtt catcactaca tacatcaata
    18601 tcaagcagaa acggtaaaat cagcaccatt ttttgagttg tatcaggatg gtgatactac
    18661 aagtgaaaaa tatattacag aaatttggat gcctgttaaa gggtgattga atagaaatgt
    18721 acttgatatg aaggcgtaat tttagttgaa gattggatct cataatttat aaagaagaac
    18781 cccgacaaag atagatatct ttgctagggt tctaattatg ttgaagtgtt atgcgacatg
    18841 ttgcttttta atcattttaa agtaaatgat ttgtagactg actaatagaa tggctgtaat
    18901 gaagataata atcatatatg gtgatgtatt aaattcgcct tttaagccaa ctaatggtgc
    18961 aacagcgcca cctaagataa attggaataa gccaagtaaa cttgatgcgt taccactgcc
    19021 acctgttcgt tcttccatag ccattgtgaa accaagcggt ccaattgacg tcacaggaca
    19081 cacatttaag aagaatgcga tgagtaagac ccaaagtggt aaatggaatg taagtgtcag
    19141 gataattaaa gcaacaccta ccacttgtat aatagttaaa atgattaata atatgtgacg
    19201 atgtaatttt tctactaata aagcaacgac ttgactgaca atgattaaac ctacaccgtt
    19261 aacagcaaac atgacactaa attgttgggg tgtcatatta tacaattttt gagtaataaa
    19321 tggcgatgca gatgaataac taaatagcat tacataagtt aaaccttgta acagcattgg
    19381 aataataaat gctggttttt tcaaaagact tccaaagtct ttaatgacgc tactaaaatt
    19441 cacctgcttt aattcatctt tagatgtttt aggtaattga gaagcgacgc caattaaaat
    19501 gatgagtgct acaatagtta aaattgtgaa aatagaacgc catgttgcta cggataaagc
    19561 taatccaccg gctaatggtg caagaatagt gatgatgcca ttaactacca ttaaagatgc
    19621 taaaaattta gcgagtgcgt tgccactaaa tttatcacca gcagaggctt tggcaatcac
    19681 gatgacgcca ccacctgtca aaccttgaat aaatcgtaaa gttaagaata atggcaattg
    19741 atcaacaaac atagacaaac ctgataccaa aatgaaaatg attaaaatgg atacagcaat
    19801 tcgtttgcga ccaaaagcat cggataaagg tccaaagata aattggccta gtgcaagacc
    19861 aatcattgtg aatgataatg ttaattgaat ttctgatgta gtagaaccaa aatcaccttg
    19921 cactttaggt aatgatggaa cgtacatatc tatagttagt gggccaaaag cggtcatgac
    19981 gcctaacgtg attagcaata aaaatgaaaa atgcttcgtt ttggttttcg aagattgcat
    20041 gatcagactc ctttaaatga tataattata taaattatat acgttttgtg tgtatttgta
    20101 tgtagttaga atacaagttt atataaaaaa tccctatact cttaatgtat catcacgaat
    20161 aacgtagtga tacagataaa gtatagggaa ttaatgttta tcatcaaaag caggtttaat
    20221 aattaattag cgccaccatt gtcatcttta aataagcttt ctaatttaga tttaatactg
    20281 tcgaattttg tattaatatc atttaacatg ctttcaattt taccttggct tgcattgttt
    20341 ttaagtgcat tatttgcctc tgtcaatttg ttgatttggt cttgataaaa ttgagttagc
    20401 ttagaatcag ctttgttttg ctcttgctgc ttcaagttgt cgatttgatt ttgtaaattt
    20461 ttaataatgt catcagtttt ttggttgttt ttaatatttt ctttagcatt atctacttgc
    20521 tggttaattt gaccgtcttg attttcaact tgttttttat aattttgaga gacttcaggg
    20581 tcattactaa ctttatcgct atcattgtta agtaaggcgc gtgtgacaaa aatagcgaca
    20641 ataattaaga gaatggcaat aatgatatat aaccattttt ttgaacggcg ggatttctcg
    20701 ttttcttctt caagaatttg ttctcttcga taatctctac cgttatagta tcgttccggg
    20761 tgttcttcag gatcgccttt agcatattgt tgtctatacg atgcgtcttc agattgacga
    20821 cgatgatttt gattttgatt gtcgtattga tatgaatcac ggtatttatc agttcttttc
    20881 atgggtttca cctcattcaa gttttaactg caaataataa agctgatgat ttactttgac
    20941 attattgtaa tttcagaata cgagcaatgt cagttgcagt gtttaataaa ttttgttcgc
    21001 tattttgtag gacatcttct aaagtgctag gtgaagagat tatagaatag gcactatcaa
    21061 taccgaaatc gtaaacatgt tgataatttt cgcctagact gccacaaatc gcgatgacag
    21121 gaatatgata ttgttttgca gctaacgcaa cgcctacggg tgttttacca aagatggtct
    21181 gataatccat gcgtccttct ccagtaataa cgaggtctgc atctttaatt ctttgatgaa
    21241 aatctgtaat gtcaaagacg acatcaatac cttttgttaa agttgtctca caaaacgcta
    21301 ataatgctgc gcccataccg ccagctgcac cagaacctgg tatttgatta acagactttc
    21361 ctgtgcacat ttttatctta tcatgataat gcgacattgc gaaatccaac tttggtatca
    21421 tctttgcatc agcgcctttt tgaggaccat aaatgtaggt agcgccgtgt tcacctagta
    21481 atggattatt aacatcacat gcaactttaa atgtaacttc ttttagcctt gtatcaagat
    21541 tggttatatc gatttgtgca atgtgagcaa gattagcgcc attcatttgt aataagtccc
    21601 cgtttacatc agtaaacttt acgcctagtg cacttagcat acctgtaccg ccgtcatttg
    21661 ttgcactgcc accaatccct aaaataatgg tcttagcacc atgatttaat gcatctttaa
    21721 ttagttcacc ggtaccatat gatgatgtgt ataaaggatt acgttcctct ttttctaata
    21781 aatccaaacc tgacgctgcc gccatttcaa taattgcaat ttgctgttcg tctgcgcgtg
    21841 cataacatgc ttcaataggt cgcattaaag ggtcattaac gatgactgta tacttagtgg
    21901 caccagttgc atgcattaaa gcatctgtgg taccttcacc accatcagcc atcggaatga
    21961 tatcataatg aagggtattc ccaaaaacat tagtaaatgc ctgctttata atattgccaa
    22021 cttgctgtgc ggtcatactt tccttaaatg agtcaggagc gatgacaatt ttttgtaatt
    22081 gcatagtaga catcctctca ttactgttgt cgttcttatt gtatataaaa ttactagtga
    22141 actaaagtaa tctctatagt gctaatgagt cgcgcgataa atcagttaca ttataatggc
    22201 gtataaggat gaggggaaag agactaaatt tgctgtgaaa gggtataaaa attgaagtaa
    22261 gtatatttga agagaagcgg attggcacgc atattcgagt atgttcgaga acgtagttat
    22321 acatatattt tgatgttgag gagttacaaa tatgaaacta acacaaacgc atgctgaaat
    22381 tttaaaattt gtcattgttg gcggcattaa tacgttgaat tattatgttg tctatttatt
    22441 gctgttaaaa ttatttcgca ttgaatatat gattagtcat attaccggat ttatagtcgc
    22501 ttttgtgatt tcttattatt tgaattgtta ttttgtctac agagtaaaac ctacttggag
    22561 aaaattcatt agttttccaa ttacgcagct tgtcaacgta agcttacaaa cagttttatt
    22621 atatgttttt gtatcctggc taaatttgcc agcggaaatt gcaccatttg caggtttagt
    22681 gattacgata cccatcacat ttgtattatc taaatggatt ttaaaagata gcaattaaag
    22741 tagcgtaaac cactaaaaaa tactttggac gggtcttaat attgcgtgaa acataataaa
    22801 aaaatgatat aatataatgg tcgcctaaca tctcgggcgt tactttaacg cagagaggag
    22861 gtgtatgacg gatgttcaat ttattaatta acatcatgac ttcagctata agcggctgtc
    22921 ttgttgcgtt ttttgcacat tggttacgaa cgcgcagcaa taaaaaaggt gacaaataac
    22981 caacacctaa ataaatcccc tcactattgc actagtgagg ggacttggtg tatttagatg
    23041 ttcaatttat tatgtaacta attatagcat taatgaacaa ggtatgcaat gtatttttac
    23101 aatattacaa ttaatgaatt gtttcacaac atggtaaagc taggatgtct ttctataatg
    23161 aaataatgca attattaatt ttaactgaca tattttatcg aatcgctgtt tatatctgcc
    23221 cgaattccgt aaggagcctt agtcatttca atagcaaggt caaaattttg ccaaaattta
    23281 tagttatacg gagaagttta aattcatgtt ttcttgaaaa acatgctcct taaatgtaaa
    23341 cttattaatt ataaaagttt acatttggat tgaggtgctt attttttatg aggattttta
    23401 ttacaagtac gaatactgat gtaggcaaaa cctatgttac aaagcattta tactatgcta
    23461 tgaaaacacg tggtcatcgt gtttgtattt tcaaaccttt tcaaactgaa gaacgccaag
    23521 acgggacgtt tccagattta gaagtattta aaaatgaatg tgatttgagc tatgacataa
    23581 cgtcactcta tacttttaag caacctgtat caccacacct tgcatttaaa atggcagatc
    23641 aaatttttct aaataagcag cgtgtattag aaaaggtgaa agttttagat aaggaatttg
    23701 atattgtctt aatcgaaggt gcaggaggca taggcgttcc actatatgaa ggtacagatg
    23761 atttctacat gactaaagat ctaatcaatg attgtgcaga ttgtgtcatc agtgtgttgc
    23821 catcaaaatt aggtgctatt agcgatgcca ttgttcacca agattatgtt aatcagaatg
    23881 tatcggcgag taatttttta ataatgaatc gctatacaga cagctatatt gaaaaagaca
    23941 atcaaatgac gattggaaaa ttaacaaata aaacagtcta tacatttgaa gaacatgcca
    24001 cgtatgaaaa tttctcagaa gcatttttaa aacaattaat aggagttaaa aatgaattac
    24061 acacaacaac ttaaacaaaa agactcagaa tatgtttggc atccatttac acaaatgggt
    24121 gtatatagca aagaagaagc aatcattatt gaaaaaggaa aaggtagcta cctttatgat
    24181 acgaatggca ataaatattt agatggctat gcttcgttgt gggttaatgt tcatggtcat
    24241 aataacaaat acttgaataa ggtaattaaa aagcaactca ataaaattgc ccattctacg
    24301 ctgctaggat catcaaatat tccatcaatc gaacttgcgg aaaaattaat cgaaatcacg
    24361 ccaagtaatc taagaaaagt attttattct gatacaggca gtgcgtctgt tgaaatcgca
    24421 ataaagatgg catatcagta ttggaaaaat attgatagag aaaaatatgc caagaaaaac
    24481 aagtttataa cgctaaatca cggctatcat ggagatacga ttggtgcggt aagtgttggt
    24541 ggtatcaaga cctttcataa aatatttaaa gacttaatat ttgaaaatat tcaagtagag
    24601 agtccgtcat tatatcgaag taattatgaa acagaagctg aaatgatggc agcgatttta
    24661 cagaatatcg agcaaatatt aattgataga aatgatgaaa tagcagggtt tattttagaa
    24721 ccattaattc aaggtgcgac aggcttattt gtccatccta aaggcttttt gaaagaagtt
    24781 gaaaaattgt gcaacaaata taatatctta ttaatttgtg atgaggtagc agttggtttt
    24841 gggagaactg gaaagatgtt tgcatgtaat catgaagatg ttcaaccgga tattatgtgt
    24901 ttaggtaagg cgattacagg tggctactta ccacttgcag ctacattgac ctctcaaaaa
    24961 atatatgatg catttttaag tgattcgcac ggtgtgaata cctttttcca tggtcataca
    25021 tatacgggaa atcaaattgt atgtacggta gctttagaaa atataaatct gtatgaaaaa
    25081 cgtaagttat tgtctcaaat taaaaaaacg tcaacaacgt tggagaaaca actacaaaca
    25141 cttaaacgac atccaaatat cggtgatgtc agaggacggg gcttaatgtt tggtgttgag
    25201 ctcgttgcag ataaagatag taaaacgccg ttagaaattg aaaaagttga acgtattgta
    25261 cgtaattgta aagaaaacgg gctaatgatt agaaatttag aaaatgtcat tacgtttgtg
    25321 ccagtgttaa gtatgtcaaa taaagaagtg aaaacgatgg tacgtatttt taaaaaggca
    25381 gtacataaca ttttagatag gaagtgttaa tatgaatttg gctaaacgca tattacaagg
    25441 ggaacaatta acaaaagaga ctgtattgaa aatttatgag gatactaata ttgatacctt
    25501 agatttatta aatgaggcgt atattttaag aaaacattat ttcggtaaaa aagtaaaatt
    25561 aaacatgatt ttaaatgcta aaagtggtat atgtcctgag aattgtgggt actgtggaca
    25621 atcacgagat attaaacaaa aacagcgata tgctttaatt ccagaggaac aaattatcga
    25681 tggagcaaag gtggcacatg ataatcatat tggaacatat tgtattgtta tgagtggtag
    25741 aggaccgagc gataaagaag ttgatcatat tagtaacact gtaagaacga ttaaatctca
    25801 acacccgcaa ctaaaaatat gtgcatgttt aggattaacg aatgacgaac aagctaagaa
    25861 acttaagtca gctggtgtag acagatataa ccacaatatt aatacaagtg aaaattacca
    25921 tgataacgtc gtgacaacgc atagttataa agatagaaca gatacgatag aactaatgaa
    25981 agcgaataat atatcaccat gttctggcgt gatttgtggt atgggagaat ctaatcaaga
    26041 tattgttgat atggcatttg ctttgaaaga aatggatgcc gacagtattc cgattaattt
    26101 tttgcatcca atcaaaggca caaagtttgg aagcatggat gatttaacac caatgaaatg
    26161 tttaagaatc gtagcattat tccgattaat caatcctacg aaagaaattc gtattgctgg
    26221 aggaagagag gtcaacttac gttcgttaca gccattagca ttaaaagcgg cgaattcaat
    26281 atttgtcggt gattatctaa ttactggtgg gcaaccgaac caattagact acgatatgat
    26341 taatgatttg ggctttgaaa ttgattatga cacttgtgaa aataaggaaa acaagaatga
    26401 tgtttcaaga gcaaattgat ttattacaac agaaagggtt atatcggtcg cttaaatcgg
    26461 tagggtatat agatagacag tatattgaag tagaaaaaga acgatttgtt aattttacat
    26521 ctaatgatta tttaggatta ggtcaaatag cctttgataa ggatgatttt gaaagattta
    26581 tgcgtaaata tagttatcat ttatcaagtt caagattgat tagtggaagt tcggtagctt
    26641 atgaagagat tgaatctatg ttatcaggtt ggctaggata tagtgcgtgc acgattttta
    26701 ataatggtta tgatgcgaat ttggcgttat ttaatatttt caaaaataca aattgtgtcg
    26761 tgttttcaga tcaagaaaat catgcgagta ttattgacgg tattaagtta agtggtttag
    26821 aaaaagtgat atataagcat ttagatattg ctgatttaga aaaaaggtta gcggaatgcc
    26881 ctaatcaaaa tatacaaaaa ataatcatat ctgatagtgt attttcaacg aatggcgacg
    26941 ttgtggatat tggtcaatta gtctcattaa agcataaata taacgcaaca ctgatactcg
    27001 atgtttcaca tagttttgga atagagaatt actcgaatta tcaaggcata gatatactca
    27061 cttctagttt atctaaagca tgtggtgcat acgggggtgt gattttaagt tcaaatgatg
    27121 tgaaggatat gttaatcaat cacggtagac cacttatcta ctcaagtagt ttgccaattt
    27181 ataatttgta ttttataaaa agaaatattg aaaagttaat aaatgctgat gatagacgca
    27241 ctaaattaaa tagtttgagt aaatatttta accaaaagtt aaaagcactc aatgttaatt
    27301 ataatagttc aaactcgccg attaagttta ttgagtttga tgacatagaa gcggctgaga
    27361 acattcatca aacattatta aagcatcatg tgtttacaag ttatttaagg tatccaactg
    27421 tgactaagcc aatgttaaga atatcattgt cgtattttca tactgaacaa gatgtagata
    27481 ggttgtttga aattttgcat caagaagatt gaggtgatcg tcatgtatag tataaaaatg
    27541 cgttctagca atcaagatgt tcatattagt ggtgctgaaa cgatttgtga gtttgataaa
    27601 atagaacaga cggtacaaag gttttataat aaaggttttt ttcatgaaaa tgggcaaccg
    27661 gattttctca atataaaaat acaaaaaatt atagaaccga ttcaacaaat aaaagcatta
    27721 caaattattg aggatgataa agcaaatctc caacatttaa cacaagaatg tggtgtcact
    27781 gaacaagcgc taaatcaagg gatgagatat attgaaaatg aaacggttta tacaggtgct
    27841 attattctat ctgctacatc tggtaaacgt ttagattcat ttggacaaag ggggattagg
    27901 gcaacgcact tttcgtttga agatataaat tgcaaagcga acttaactga aagagtgaag
    27961 gatgccttag caattgcaag ttgtgtcaat gcgcattcgt atgtcaaagg agaactttgt
    28021 gtgtctgatg acttaacgta tacaacagga tattttgcct ctgctaaaat tggttaccat
    28081 cgattatttg atattaaacc agttaatacg agatatggag gcagaataat atttgtagac
    28141 gatagtattg atttagatca ttacatatca tttttagaaa gcacaccgaa gcaagttatt
    28201 tatgaaacgg tataggagtt ttagtatgac atcaaaagat atgacgcaaa taagtgcgat
    28261 tgcagcgata ttaacgattt tagcagtttt gaaaatacct tctattttac cgggactaga
    28321 ttttcaatta tctgcaccag tggcattatt gatactagct ttctttggaa ttaaaaaata
    28381 ttttttaggt ggattgttat ctagtatcat tttgcttata ctcggcgtat ttaatccaat
    28441 caatgtgatg atatctttaa cttttagatt aattgcaata tttgtagttt atttattgaa
    28501 aataaatgta ctttcgttag ttttagcaag tgttttggga agtttattat ctagattatt
    28561 gttatcgatc attttaaatt tacctgtatg ggcggtattg ctaaacacga ttccaggaat
    28621 tatatttaca attatattgg caattccatt gtatttaact ttaagaaaaa gaatggcagt
    28681 cttattaaga taataaatta aaaaacggtc gtctcaatta atagtgacga ccgtttttcc
    28741 tacctattta ttgatttcag tttcttttgt atctaacaat ttcactttgt gattttccca
    28801 atcaatttca tatgttgatt taaatgttct tgttttaaag tttttataat tcgcgcctgc
    28861 ccagtagaag ccattccaac gaatttggta taagtccatt tcacgctgat atgttacggt
    28921 gattttagat tttttagcgc cattttgttt atgtgacaat acacttaaaa attccggatt
    28981 aaagttactt cttgataata atggcatttg atgttgcgca atgaagtttt ggcctgcgta
    29041 tgaactgctt tgtctaccag ccaagaagag ttcattgcca tatgttggat ggaaactatc
    29101 tcgtccataa ggtccccaac cgttattcat aattttatgt gcttcaacac cccagccaac
    29161 atttttataa tttgtgttgc ggcttaatgt tgttctatag ctttcttgtt tataattaat
    29221 tgtttctgaa aacgcagtgt ttccgttaag tccaccagat aagccattag agatactaat
    29281 gtctccaccg aatgtgtaac ctaaagtatt ttggacttga aactcttcat tttgattttt
    29341 aggtgcataa tcaacgacgt ttactgaatc gttagattgt gaactaattg atacattata
    29401 ttttgcaccc caatataatt ttgaaaaatc atagtcttta gggttaggtt tttcataacc
    29461 tgagttaata ttacctgcag ctttaagcac taaagtatct ttatcataac ttttatcttt
    29521 gatgaaattg aatgttaaaa tttgtgaaat tttaaattta tcagaatctg ctgtggctgt
    29581 tgttttgtat agagtaactt tgtcatcgac ttttttgacg cttactggtg tgattttgcc
    29641 ttcagcatta gcagtattag aaagtagtaa taatgccata gacgtagcaa cggatgattt
    29701 gactaattta ttcattttca tatcaattct gtcctttcac cttgatttca tgagttttcc
    29761 aattgacctc gtatttcaca gtatagtttc tatttacaaa tgcattatgg actctatgtc
    29821 catctaaata actgttgcca taatgtgttg atcttttaat ggcgtgagtg acgtccatat
    29881 ttctaccgta agtaatttca aactcgcttg tatcacctga tcctttttca tgagacactg
    29941 ttgcgataaa tgaagggtta aaaccacttt gtactaaagg tggcaattcg ctgtctggaa
    30001 caaaataatc tctagggtct ttactgtgag gtttataacc tacaaataga tcgctatcaa
    30061 atgctgactt ctgacctgat tcagttgcaa atgaatttgc tttaacgccc catagaacac
    30121 ctttagagtt ttgttgttct acttcactga cataattttg ttgtgtatag ctaatcgatt
    30181 tagagtagtt aaatgatcca ttaccaccga gtgatggggc tgactggaaa ttaccaccga
    30241 tattgtatcc taatgtctga ctaacatttg tagattcaat tttattttta ggtaaataat
    30301 taattaatga gacattaggg tcgtttgttt ttaaaccaat attgtattgg aagggccaac
    30361 gcatagcttt aacatgatta ctttttttat agttgtaata tgctgttcta gagctaataa
    30421 agccttgcat ttttaaaatt aaagcatctt tgttatattt tttatccttt acaaagtcga
    30481 attggatgtt ttgagttaca ccccatttat tacttgtttt atcttctgtc cttttgataa
    30541 tttctacatc gcttccttta ccgatgtctt ctgtatcgtt agcagcttta gcattttcga
    30601 ataacggagt ggcaagggga gcaattaaac taatagataa tgttgtagct aacattttat
    30661 ttttaagcat aagtttcact ttctttctat aattttattt aacttaatta aataataata
    30721 tattttgaac gttattataa ttaactgttc attaacaatt gactattatc aactttcttt
    30781 gaaattaagt taaaaatgcc ataattccgt tcaataatat ctttttgttt ggaatgacaa
    30841 aaactagttt tgtgagaaat acacttaata attccttact attgttgaca gctataaaca
    30901 ttttttagga aattgcttac acgaatatct taaatttatt tttaattaat gtttgtaaaa
    30961 ataaaatttt gaattttgag attttattta tctactgcta aaatttaatt atttattaac
    31021 tgtagaataa ttatttaagt tttaatttca aattgattcg tttgtattat tagtgtatct
    31081 attattgtgc gatactttgt tcttataaaa atcagttgtt attattaaat ttaatgtacg
    31141 ataaaataat ttaatgttat gaggctgatt caatatgtta aaaaatcctg agacaagtat
    31201 gaagtgtccc aggattttgt aaaatgattg aactgtttac ttaggtgtga tgcttttaat
    31261 ttttacttca tgtgttttcc agttcacttc atatttaact gtaacgtttc ggtttttaaa
    31321 agcatcatgt tttctatcaa cggctaaacg atgtcttgtc acgtatgcat atgtagcatc
    31381 catgtttctg ccgtaagtga tttcaaactc gcttttatca cctttacctc tttcgtgtga
    31441 caatgttgta ataaatgatg gattaaagcc actttgaatt aaaggaggta gttgattatc
    31501 tgggacgaaa tagtctcttg ctgctggacc agttgggtct tgtgcgaata aatattgatc
    31561 ataggcagaa acttgaccat tcggtgtaac gaatgaattt gctttaatac cccatttaac
    31621 acctttagag ttctggcttt ctacttcagt aacatagttt ttttgattat agctaatggt
    31681 tttagagtag ttgaatgagc cactgcctcc aattgatggg gctgtttgga agttgccgcc
    31741 aatattatag cctaatttct ggcttacatc tgctgagtct attttatttt taggaaggta
    31801 attaattaaa tcaacattag ggtcttttgt ttttaaacta atattatatt ggaaaggcca
    31861 tatcattctt ttaatatatg gatatttttt taagtctgaa taagtagttc tagagctaat
    31921 gaagccttgc atcttaacaa ctagggcatc tttgttatat tttttatcct ttacaaaatc
    31981 aaattgaatg ttttgagtta tagctaatcg tttgctagta atgtcttgtg ttcttttgat
    32041 gatttctgca ccttggccga tatcttctat cttattttct gctttagaaa tttctataaa
    32101 tggattggct aaaggtgcga ttaaaccaac tgctaaagtt gctgttaata ttttattttt
    32161 aatcatagaa atcactttct ttctatttaa ttttaagttc atatatataa taatctattt
    32221 gtaagcgttt tcgaattaac tgttcgttaa aagttaatta ctatcaacat gtattgaaga
    32281 gacattttta gcattgtcaa atgatgtttt tgcattatgt tatgtttaag agatttattg
    32341 gttgcaggcg tttatatagc tacataacta tgttttgcaa gtcttattct attgaattgt
    32401 tgtttaatta tatggtgctt gtagtggaag aggaatggat tgcgcaatag tttgtgaaaa
    32461 tttgaagtta ataaagttga gctgattcaa ctaaatgatg aactattcgt taacgatgta
    32521 aggattattt aacaagttgc aggtaaaaaa gtgggctgat aaatagcgaa gaaacattta
    32581 attgatggaa aatgaaaaag aaccgcagat tcgattgaga atcaacggtt ctttttaatc
    32641 acggtttatt acgaaaatta tttaaaaaat gatgtaaaac tagtgaagat atttttgatt
    32701 gattatttcc agaatgagtt aaataatcca gttattgtat ttttgattga tgatgcagtt
    32761 tcttgagctt taatagctgt ttttactaat gttgggtttt ggtagaaagt atataatact
    32821 ttaccagtat tgattgactc agttacatag tttcttactt gtgcatatga tttagctaaa
    32881 ttcccgcttt ctttgcttac ttcaagaggt ttaatgtatg aaccataacc actaccgatt
    32941 gttgttaaaa ctgtttggtc aattaaacct ttatattggt aatatttatt atagtagtac
    33001 tttgctgtat catatttttc tttatagctt ttataagtat ctgttaaata cttataacca
    33061 tagttaaatg aatcttttaa tgattgatag tagcctagta attttgattg aggaacttca
    33121 actttaggtg attgaatttt aggaacctca actttaggtg attcagcttt aggtttttca
    33181 atttgtggtg attgaatttg agggacctca acttttggtg atgatttctt tttagcatct
    33241 ttttgagcta ctaatgtatc taattgtttt tgtaagtgtt ctttaacatc cattggtgct
    33301 ttgttaactt cacgttgtgc taaacgtctg ttttcaattg aatcatcttt ttgtaatttt
    33361 gtgattgcat catttgctgc tttaacacgt tcatcatgac gaacgattgc tttttcaata
    33421 gaaacttttt ctacaaaacg attgtttttg tcgcgtaaat ctttaggcaa ttttgaattt
    33481 gcttcatatg ctgctgattt atcatgtggc gctttgttat cttggaaatc ttggattgcc
    33541 ttattagcat tttcaatatt ttgacgttct tttgctttag aagcttgaac tgattcaacc
    33601 caaacttgtt ggcttctatc tggattttct ttaatttgtg caatgtaatt atttttttct
    33661 tgttcagtta agttatcatt tttaagaaca ttgtaaaaag cgttttgttg tgcaacacgt
    33721 cggtctgggt tcttgctgtc tttaagtgat tcagagaata cttcttgtgc acgttctggg
    33781 tgttcgcgta atgttttgat gtattggtta cgttgttctt ctgtgatgcc tttcaaatgt
    33841 aatacttggt aaaatgcttt ttgttgatca gttacatagt tgttttgagt tgtttgttgt
    33901 ttagttgaag tttgttgagt gttttcactc gcttttgctt ccccatttga aatcattgta
    33961 gctaaagtaa ttgttgctgc cccaactagc aacttcgata tatatttatt tttcatgtgt
    34021 attccctttc tttttacata ttaaatttat tgtatgcttt tagaatttat cacatgagaa
    34081 tacaaatgga aattaactaa ttattaacta atgttaacta atagattaac tagtgttaac
    34141 tatgtaaaaa agcgtttaat tcagaacatt cagcgtattt taagtaagga agtactatat
    34201 ggagaaatgc aatagtttgc taataaatag aggagaaaat tgtctttcga agagagtttg
    34261 gtagagtaga aagctcattt aatttgtcac tgatgcactt tttcttctat ataattaggt
    34321 cacgacatta gatagcttcg caactaatgc atagggtccc aacatagaaa ctttcgagac
    34381 gaaagttaac aaacagtgag agttggacgc ctttctagct cataaaattc gcagaaaata
    34441 aagggacgca gttggacgta aaacgtcaac tgcataagcc tttctagctc ataaaattcg
    34501 cagaaaataa aggaacgcag ttggacgtaa aacgtcaact gcataagcct ttctaacaaa
    34561 taaatttgaa gaaaggctaa aaaatatttt taggggttgc gaatgaataa attagtttaa
    34621 ttttataaaa atttataaaa attaaaaacg ttgtaaattt aaaataaatc attcataata
    34681 ttgttactat actaattcaa ggtggttcaa cgcatgtctc ataatgaaaa tcttaaattg
    34741 gcgcaacacg gtgcttattt gagtttaatt gtatatataa tcttatctat tgttaagtat
    34801 gtcacgggtt ttgtctttaa ctctgcagct gtaagagccg acgcattaaa caatatgaca
    34861 gatattatcg tttctttagc tgttattatt ggattgaaaa tttcaattaa acctgccgat
    34921 cgaaatcatc cttatggcca tttgaagtct gaaaatattt cttcattatt ggtgtcattt
    34981 gtcattatgt ttgtaggtat ccaagtagtt attcaaaatg cacctcgttt gttcaaagaa
    35041 gatgacgttg tacctaacgc aataacaatt atcgtcagct taatcagtgg tcttgtaatg
    35101 ttgattgtat ttgcagtcaa tcaaagacta gcgaaaagaa cgaaaagtag ttctttaaat
    35161 tctgctgcca aagataattt atcagatagt ttagtcagca ttggaacggc gataggatta
    35221 atttttacgc aaatcggttt cccaattgtc gatattattt tagcaacatt gctaggctta
    35281 ctaatcgttt atactggttt tggtattttt aaagaagcaa tttttatgtt gagtgatggt
    35341 ttcaatgaaa ctgaattaga agcgtatcgc aatgatattt tagaagttga tgaagtacaa
    35401 gaagttaaaa gtattaaagg gcgttaccat ggaagtagtg tgtttattga tgtcacaatt
    35461 gttgtagatg ctaacctttc cttagtagaa gcgcatcaaa tttgtgacaa tgtcgaacat
    35521 catttgcaca aaaaaggtat ttcctcagta tacgttcacc cagaaccaga ccatctataa
    35581 tgtatttaaa cgtcttcata gatatataaa tatctgagga ggcgttttta ttacgtttat
    35641 ttttaggtgc gcctaaaaat attttttaaa ctttagaaaa aatagcgaac aaaggtgtat
    35701 aatatgattt gttattaggt ggttattgat tcatttatga attaaaaaat gaaaatgggg
    35761 aataggattt atgtatttaa tatttagtga taattgattt aatcattgaa ttttaaaaca
    35821 tattgaaaag aaaattacta agattaaatc atcttaaaat atccctgaaa taacgtccta
    35881 aagattaaag gaaagaggtt ataagttatg ccaaaattaa ttttatgtcg tcatggacaa
    35941 agcgagtgga atgctaaaaa cttatttact ggatgggaag atgttaattt atctgaacaa
    36001 ggtattaatg aagcgactag agcaggtgaa aaagtaagag aaaataacat tgccatcgat
    36061 gtagctttta catcgttatt aacacgtgct ttagatacaa cgcattatat tttaactgaa
    36121 tctaaacaac aatggattcc tgtatataaa agctggcgtt taaatgaacg ccactatggt
    36181 ggattgcaag gcttaaataa agatgatgct agaaaagaat ttggagaaga acaagtacat
    36241 atttggcgtc gttcttatga tgtgaaaccg cctgctgaaa ccgaagaaca acgtgaagct
    36301 tacttagctg atcgtcgata taatcattta gataaacgta tgatgcctta ttctgaaagt
    36361 ctgaaagata ctttagttcg agtgatacca ttttggacag atcatatttc acaatatttg
    36421 ctagatggtc aaacggtatt agtttctgca cacggaaatt caattcgcgc attgattaaa
    36481 tatcttgaag atgtgtcaga tgaagatatc attaattatg aaattaaaac aggtgcaccg
    36541 cttgtttatg aattaacgga tgatttagaa gttatagata aatactactt ataaaaaaag
    36601 agctgcatgt acacaaggag tgagtgtata tgcagctctt aaatatgtga agtaatgtaa
    36661 ggaaatagtt aagtatagag tttatattaa cgagctaggg atactcgaaa atatagttag
    36721 acatacaata tagtcaaatt aaaacaatta tttcgctctt ttatgttgct taataatctt
    36781 taaagcacgc tttcttgttt taatgttagg gctatttaaa ttacgacgag cagtctgtaa
    36841 atctaatttc atctctatcc ctccttgtaa atatattatg accgataact actcatatgt
    36901 aaatagtaat gattacgttt taaagaaatt gtaataaagt cgtgctaatt tttttgaaaa
    36961 tgggtataat taccggatat ctaaaaatga gtgtcgtttt ttagatggtg agggggaagc
    37021 tttaaatgtc gaagaaacaa aaattaacga tgattattac tatgctgatg ggtggatttt
    37081 ttggattatt aaatgaaaca ctattagtga cggctttacc aagtattatg aaagattttg
    37141 aaatttcata tacacaagtt caatggctga caacagcttt tttattgact aatgggattg
    37201 ttattccttt gtccgcgctt gttatacaac gttatacaac aagacaagtg tttttagtgg
    37261 gtatttctat ctttttctta ggtacattac tcggcggctt gagtccgcac tttgcaacat
    37321 tgttagttgc tagaattatt caggcgttag gcgcaggtat tatgatgcca ttgatgatga
    37381 caacgatttt ggatgttttc caaccgcatg aacgcggtaa atatatgggg atatttggtt
    37441 tggtaattgg tttagcacca gctattggac ctactctttc aggttacctt gttgaatatt
    37501 ttaactggag atcgcttttc catgttgtcg ctccaattgc agctgtgaca tttttaattg
    37561 gatttaaaac gataaaaaat gttggaacta caattaaagt acctattgat tttatttctg
    37621 tcattttttc tgtactaggt ttcggcgggt tattgtatgg aacgagttca atttcagaaa
    37681 aaggttttga taatcctatc gtattagtat ctatgattgg aggcgttgtt ttagtcgcat
    37741 tatttgtatt acgtcaatat cggctatcaa caccattatt aaattttgct gtatttaaaa
    37801 ataaacaatt tacagttggt atcattatta tgggtgtcac aatggtatcg atgattggtt
    37861 cggaaacgat tttacctatc tttgtgcaaa atttattgca tcgttcagct ttagattctg
    37921 ggttaacttt attgccagga gcaattgtta tggcatttat gtcgatgact tcgggtgctt
    37981 tgtatgaaag gtttggtcct agaaagcttg ctttagtagg tatggcgatt gttgttatta
    38041 ctacggctta ttttgttgta atggatgaac aaacatcaac aattatgttg gcaacagttt
    38101 atgcgattcg aatggttggt atcgcgttag gattaattcc agtaatgacc catacgatga
    38161 atcaattaaa gccagaaatg aatgcacacg gttcatctat gacaaataca gtacaacaaa
    38221 ttgctggttc tatcggaaca gctgcattaa ttactatttt atcccatgca agcaaaaact
    38281 tttctccaac tatgtcagac tataatggca tgaacaaaat tgatatgatg aatcaaatta
    38341 aagttgatac gatgttgcat ggttatcacg ctggattttt atttgcatta ttgattacag
    38401 ttgttagttt cttttgttca tttatgttac aagggaagaa aaaagaagtt gattctcgtc
    38461 aataaatata agttgctagc tatataaaga tttttagcaa aagttcaaca tattgactta
    38521 tccggcattt cagattaaaa tatttttatt ccgattagaa taataagaat aaggagatat
    38581 attctatgaa aagactttta tttgtgatga tagctttcgt tttcatattg gctgcatgcg
    38641 gaaacaattc gtcgaaagac aaggaagcta gtaaagatag caagacaatt aatgttggta
    38701 ctgaggggac ttatgcacca tttagtttcc acgataaaga tggtaaatta actggttatg
    38761 atattgatgt tattaaagca gtggctaaag aagaaggttt aaaacttaaa tttaatgaaa
    38821 cttcttggga ttctatgttt gcaggtttag acgcagggcg ttttgatgta atcgcgaacc
    38881 aagtaggtat taatcctgat agagaaaaga aatataaatt ttctaagcct tacacattct
    38941 caagtgctgt tttagttatt cgtgaaaatg aaaaagatat taaagatttt gatgatgtta
    39001 aaggtaagaa gttagcacaa acattcacat ctaattatgg taaattagct aaggataaag
    39061 gtgctgatat tacaaaagtt gatggcttta accaatcaat ggatttatta ttgtctaagc
    39121 gtgttgatgg tacatttaat gatagtctgt catacttgga ttataaaaaa caaaaaccta
    39181 atgctaagat caaagcaatc aaaggtaatg ctgaacaaag tagatctgca tttgcatttt
    39241 ctaaaaaagc agatgatgaa acagttcaaa aattcaacga tggcttgaaa aaaatcgagg
    39301 aaaacggtga attagctaaa ataggtaaga aatggtttgg tcaagatgtt tctaaatcta
    39361 aatagcgaac aacaacatgc gctagatgct gcaaaacaag ctttcggtcc tatgctagaa
    39421 ggtttggtca aatattcaat tcctattaca ttagttacat ttgttttagg attgattatt
    39481 gcattattta cagcattaat gcgaatttca acgagtaaaa ttttaagaag tatttcacgt
    39541 gtctatgtat ctattattcg aggaacacca atgatagtac aactatttat catattttat
    39601 ggtattccag aattaggtag attattaaca aatgacgctg acaaccaatg gacattggca
    39661 cctgtagtgg ctgctattat tggtttatca ttaaatgtag gtgcgtatgc ttcggaaatt
    39721 attcgtggcg gtattatttc tataccgaaa ggacaaacag aagctgcata ttctatcggt
    39781 atgacgtatg gtcaaacgat acaacgtatc attttaccgc aggcaattcg agtgtcgatt
    39841 cctgcactag gtaatacatt tttaagttta atcaaagata catcattatt aggatttatt
    39901 ttagtggctg aaatgtttag aaaagctcaa gaagttgcgt ctacaacata tgaatattta
    39961 acaatttatg tgttagttgc gctaatgtac tgggtggtat gctttattat ttcaattatc
    40021 caaggtatct atgaatctta tattgaaaga gggtatcgct catgattcaa ttgaacaata
    40081 tccataaatc atttaatgat gttgaagtca tcaaaggtat tgatttatct gttgaacaag
    40141 gtgaggttgt aaccttaatc ggtcgatctg gttcaggcaa aacaacattg ttacgtatga
    40201 ttaatgcgtt agaaattcca actgaaggta cagtttatgt taacggcaaa acatatacat
    40261 ctaaagataa aaaatcacaa atagaagttc gtaaacagtc tggtatggta tttcaaagtt
    40321 ataacctttt tccgcataag acggcattag aaaatgtaat ggaaggtctt atcacagtta
    40381 aaaagttgaa aaaggatgag gcacgtggga aatcacttga gttacttgag aaagttggtt
    40441 taacacatgt caaagatcaa cgtccacatg cattatcagg tggtcaacaa caacgtgttg
    40501 ctattgcaag agcactagca atgaacccta aagtgatgtt gtttgatgaa ccaacatctg
    40561 cacttgatcc tgaacttgtg aatgatgttt taaaggttat taaagatttg gctaatgaag
    40621 gcatgacaat ggtcattgtg acacatgaaa tgcgttttgc taaagaagta tctaataaca
    40681 ttgtatttat tcatgaaggc atgatcggag aacaagggac tccagaagag atgttcaatc
    40741 gtccgaaaac agaagaatta agacgtttct taaatgttat aaatgaagaa taatcagata
    40801 aaaccacgta tcatgtttta gtatggcgat gaagccatat acatgatgcg tggttctttg
    40861 ttatgttgtc ataatcttgg agcgatattt taacgacgtt tatgatttaa gtacgtttgt
    40921 accttatata gtaatggttt gataggctta ataaagtcac cgatatactc ttctacatgt
    40981 gcgttaaagc cttttttgaa ttgttgtacg ccataatctt ctgatgactc tgaaaagtca
    41041 ccggtaatac catagaaatt atagcgatca atatgatgcg ctttagcaaa cttaatcatt
    41101 tcccactgca aatggtaggc acccatataa gcattatatt tagggtttga accactagat
    41161 aagtaataaa cttcatgctc attgtagata aacaaagcag aagctaagtt taagactgca
    41221 ccatcttgtt caatttgttc tattgtattg tcgattttac gcttattgct atttagctgt
    41281 tgttctagct gtgtgcgttt cgttttattt ttctttgaat taggactttc ttccaatgcc
    41341 tcttcaacac ctgagagttc agctgttaat tgctgttgct ttaattgtaa cgtttttaaa
    41401 tactccttta aatcaatata cgccaacttt aacatggcgt ggtcatcgta taacttttgc
    41461 atttcttcaa agtatggtaa ctcacggaat ttgaaaccat gcttttcttc ggccatatgg
    41521 aataagtcga aaaaagtttg cgtttcatca atcgttaacg ttttagtttt gacaccaata
    41581 tcatatgttt ttttaatatt acgtctcgtt tgataatcca tttcttttaa taactggtct
    41641 tctgttttat cctttaaatc taatacagat aaccaacgga tttgactcat tgaatcataa
    41701 ccgactggaa aaccttgatg cttatagcct aatgtgtcca ttgttctaac aaatgctcgg
    41761 ttgtcataag acttaacaat ttcaccgtct gcattgcgta aattttcaat taaatatgga
    41821 tctacaagga catataaaca attttgtttt tttaaatatg acgttaatgc tttaaagaaa
    41881 aatgctacta atgattgatt tgtataatcc atcactggcc cgcgatgtgt ataaaaatat
    41941 ttgaaaaatt taagtgtgcg tgcttctgtc aataagcatc ctgcaatcac ttccccattg
    42001 tcatctttaa cccctacaag atgcacatcg ccttttaaat caactctatg attgtaatga
    42061 atagctgatt gtgtgtaatg tgaaaaatgc tttgttgtaa acgcattgaa ctcatccgaa
    42121 gtcaacgtta caaagttcat tttatcccat ccctctctga attgaactgt aatttttgaa
    42181 tttattaata atgctatttt tttattttat caaaaactga ttgcaaatac acatagaaaa
    42241 tgaaatatat ttttctttga ctatacataa ctgttatttc tttgaatatt tatattaaat
    42301 gcattggtag tagttgtagc gcattgatgt ttgacggtgt atagtattaa ttatattaaa
    42361 agttagttgg aagttgatag taggagtggg agcattgaag cgatttgtgg cgacggtatt
    42421 attattacta gtctttatat caggatgtgg taatgataaa tatgtgaaag aaatagatga
    42481 agcagttaaa attcaaaatc aaaaacaaga acaacttgcc aaaaaaggca acggtgatcg
    42541 tgttgatcat tttgaacgca aagatgctaa tatttatgtc tacgataagg ataaaattat
    42601 cattttagct tataaacctt tgagtaatga tgatgaagtg cattattatg catatgattt
    42661 tagtgataaa cgtgtatcat ataagcaaga ttttgattcg agacgatatt atcaacaaca
    42721 tgatgcggat tatcatgaag aaaatatgac gaactagata tgaacaggag ttattatgac
    42781 taaaaaatta ctaacattat ttatagtgag catgttaatt ttaacagctt gcggtaaaaa
    42841 agaatcagca acgacatctt cgaaaagtgg taagccatta attgtcatct atggcgacta
    42901 taaatgtcct tattgtaaag aattagatga aaaagtcatg ccaaagttgc gtaaaaatta
    42961 tatagataat cacaaagtgg aataccaatt tgtcaattta gctttcttag gtaaagactc
    43021 aattgttggt tcgcgtgcga gtcatgcagt attgatgtat gcacctaaat catttttaga
    43081 ttttcaaaag caattatttg ctgcccagca agatgaaaat aaagaatggt taacaaaaga
    43141 actattagat aagcatatta aacaactgca tttagataaa gagacggaaa ataaaattat
    43201 aaaagattac aagacaaaag atagcaagtc ttggaaagct gcagagaaag ataaaaaaat
    43261 agctaaagat aatcatataa aaacgacacc aactgcattt attaatggcg agaaagttga
    43321 agatccatat gattatgaaa gttatgaaaa attattaaaa gataaaatca aatagtctta
    43381 attgttgaga taagtaagtg ataccaattt attaggacaa tatgaaaggc aatttctatt
    43441 agcgatatga tagagattgt ctttttaatg tatgaagtct aaataggtgg aaattatcat
    43501 gtaaacattg tttagtaggg aatcagtaca ggataggtat tgattcttcc ccgtgcaaaa
    43561 tcttgattag tagttttttt ctgtgccgct tgttttattg tacagatatt tgtacaattg
    43621 aggtgtgtta aaaggaggcg ataaatatga ttattaaaaa ttattcatac gctcgacaga
    43681 atttaaaggc acttatgaca aaagtaaatg atgatagtga tatggtaact gtaacatcta
    43741 ctgatgataa aaacgtagta atcatgtcag aatcagatta taactccatg atggaaacac
    43801 tttacctcca acagaaccca aataatgctg aacacttagc tcaatcaatt gcagatctag
    43861 aacgtgggaa aactataacg aaagatatag atgtataatg gctaggttaa atattacgtt
    43921 ttcgcctcaa gcctttgaag attataagta ttttcagcag aacgataaaa aaatggtgaa
    43981 gaagattaat gagttactta aaagtattga cagaaatggt gcattgaaag gtataggtaa
    44041 gcctgaaaag ttaaaatcga atctgactgg gtattatagt agacgtatca atcacgaaca
    44101 tagattggtt tatacagtag atgacaatca tataaaaata gcatcatgta aataccatta
    44161 ttaaatctaa aagtcacact ataaaaatag ggtaagttat tttaggacaa tatgaaaagg
    44221 caatttctat tagcaatatg atagagattg tcttttttct tatattacga cgttcgtaaa
    44281 tactataaag ttttctgtat catatttgta caatagtcat gtattagata aaacgaaaag
    44341 tggtaaatac ctaaagtatg attcgacttt atattattta taaaaacagt gcttgtattt
    44401 taagtagcat gagtatataa ttttaaaacg taaagattac tatttaggag ggtgactatg
    44461 aaaaagaaat taggtatgtt acttcttgta ccagccgtaa ctttatcatt agccgcatgt
    44521 gggaatgatg atggaaaaga taaagatggc aaggtaacaa ttaaaacgac agtttatcca
    44581 ttgcaatcat ttgcagagca aattggtgga aaacacgtga aggtatcatc aatctatcca
    44641 gcagggacag atttacatag ctatgaacca acacaaaaag atatattaag cgcaagcaag
    44701 tcagacttgt ttatgtatac aggggataat ttagatccgg ttgctaagaa agttgcatct
    44761 actatcaaag ataaagataa aaaactgtct ttagaagata aattagataa agcaaagctt
    44821 ttaactgatc aacacgaaca tggtgaagag catgaacatg agggacatga tcatgagaaa
    44881 gaagaacatc atcatcatgg tggatatgat ccacatgtat ggttagatcc taaaattaac
    44941 caaactttcg ctaaagaaat taaagatgaa ttagtgaaaa aagatccaaa acataaagat
    45001 gactatgaga aaaactacaa aaaattaaac gacgatctta agaaaattga taacgatatg
    45061 aagcaagtta caaaagataa gcaaggtaat gcagtattca tttcacatga atcaattgga
    45121 tacttagctg atcgttatgg ttttgttcaa aaaggtattc aaaatatgaa tgctgaagat
    45181 ccatcacaaa aagaattaac taaaattgtt aaagaaatta gagatagcaa tgctaaatat
    45241 attctttacg aagataatgt tgcgaataaa gtgactgaaa caattcgtaa agaaacagat
    45301 gcgaagcctt taaaattcta caacatggag tctttaaata aagaacaaca gaaaaaagat
    45361 aatattacgt atcaatcatt aatgaaatct aatattgaaa atatcggtaa agctttagac
    45421 agtggtgtta aagtgaaaga cgacaaagct gaaagtaaac acgacaaagc aatttctgat
    45481 gggtatttta aagatgagca agttaaagac cgtgaattaa gcgattatgc tggtgaatgg
    45541 caatctgttt acccttactt aaaagacggt acgcttgatg aagtgatgga acataaagct
    45601 gaaaatgatc cgaagaaatc tgctaaagat ttaaaagctt attatgacaa aggatataaa
    45661 actgatatta ctaacattga tataaaagga aatgaaatta catttactaa agatggtaag
    45721 aaacacactg gtaaatatga atacaatggt aagaaaacat tgaaatatcc taaaggtaac
    45781 cgtggcgtga gatttatgtt taaattggtc gatggtaatg ataaagactt accgaaattc
    45841 atccaattta gcgatcacaa cattgcacct aaaaaggcag aacacttcca tatctttatg
    45901 ggtaatgata atgacgcgtt attaaaagaa atggataact ggccaacata ttatccttca
    45961 aaattaaata aagaccaaat caaagaagaa atgttagcgc attaattcaa caggtttgca
    46021 cactctactc aactgttaat agatgaatag agtgtgtttt ttatttcgta gttatcaatt
    46081 tcaacagagg gaatgattaa tattactata gtttataaca aatggtttgg taacatatgt
    46141 tactcaatgc atgttaagat gagtaaatat aaaagatagg gagtgggaag atgacgatta
    46201 aagttgaaga taataaaatt attttttcaa gaacgataga agcaccgatt gaaaaggtgt
    46261 ttgatgccta tacgacaaag gcattatttg aaaaatggtt tcatccaaaa gacgctagta
    46321 ccaaagtgtt tcgctttaat gctgtttcag gcggtgatgc attttacgcg attaaaacac
    46381 ctacaatgac aagctataca ttagcagaat ataaaacggt taagcgtcca tatttaattg
    46441 aatatattga ctcatttgcg acacctcaag gtgcaaaaga tacaaaaatg ccaagcatga
    46501 agattacttt gtcattttcc gagttcaata caacgaaaac gacagtgaca tcaacatcgg
    46561 tatttccaac aaaagaagcc gctcaacaag tcataaacat gggtgttgag caagggatga
    46621 atcaaacatt agatcaactt gatgctttat tgaaataaag ttgttgaaaa tatgtaaaaa
    46681 attttatatg ccattcagtt tataataata acgtaatatt attaattgaa tgaaggatga
    46741 ttcgatggca caacgttaca ttaaagtatt aatattatat atttttacta cgcttttatc
    46801 ttcaa
//
LOCUS       DAHHRR010000008        46524 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.008, whole genome shotgun sequence.
ACCESSION   DAHHRR010000008 DAHHRR010000000
VERSION     DAHHRR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 46524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46524
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.008"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..654
                     /locus_tag="NBI42_000346"
     CDS             <1..654
                     /locus_tag="NBI42_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HCD1776810.1"
                     /translation="LFLLGYFLPIGIDKVKNLSYSQFFFSSYTVATQLGFLLFSFVIA
                     YFINKEYSNKNILFYKLIGDNIFTFFYKKVSVLFFECLVFIILSITIISIIYSNFSHY
                     LLLIILFSLVILQYILVVGTISMVSPNILISLGISIVYWIGSVILVAINKNIFGIVAP
                     FEASNTMYRAVEKILNNESTFICPTEIINTVLFFVLLLIVNTIVLLLSRKRWLKIGM"
     gene            complement(816..1436)
                     /gene="srtA"
                     /locus_tag="NBI42_000347"
     CDS             complement(816..1436)
                     /gene="srtA"
                     /locus_tag="NBI42_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HCD1776811.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            1630..2121
                     /locus_tag="NBI42_000348"
     CDS             1630..2121
                     /locus_tag="NBI42_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1776812.1"
                     /translation="MIRCAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERIAWFET
                     KQHNHEPIFVFESDGNVLGFATFGSFRPWPAYQYTIEHSIYVDASARGKGIASQLLQH
                     LIVEAKAKGYRALIAGIDASNKASIQLHQKFGFKHAGTLTNVGFKFNQWLDLAFYELD
                     LQD"
     gene            complement(2270..2440)
                     /locus_tag="NBI42_000349"
     CDS             complement(2270..2440)
                     /locus_tag="NBI42_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776813.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(2502..2600)
                     /locus_tag="NBI42_000350"
     CDS             complement(2502..2600)
                     /locus_tag="NBI42_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776814.1"
                     /translation="MDIKKHLSGLLVPDRCFLIIYDEVEKFMANAC"
     gene            complement(2618..3517)
                     /gene="sdaAA"
                     /locus_tag="NBI42_000351"
     CDS             complement(2618..3517)
                     /gene="sdaAA"
                     /locus_tag="NBI42_000351"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCD1776815.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(3531..4211)
                     /gene="sdaAB"
                     /locus_tag="NBI42_000352"
     CDS             complement(3531..4211)
                     /gene="sdaAB"
                     /locus_tag="NBI42_000352"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCD1776816.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLQVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(4214..5257)
                     /locus_tag="NBI42_000353"
     CDS             complement(4214..5257)
                     /locus_tag="NBI42_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCD1776817.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            5806..6009
                     /locus_tag="NBI42_000354"
     CDS             5806..6009
                     /locus_tag="NBI42_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776818.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            6192..7127
                     /locus_tag="NBI42_000355"
     CDS             6192..7127
                     /locus_tag="NBI42_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCD1776819.1"
                     /translation="MRYLVNNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGIAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPKEIDE"
     gene            complement(7209..8177)
                     /locus_tag="NBI42_000356"
     CDS             complement(7209..8177)
                     /locus_tag="NBI42_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1776820.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRSKR
                     RFMYKEEAKKALEKMAPQTAGEYEGTNYQFKMPVKMDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQIKNIIN"
     gene            complement(8310..8654)
                     /locus_tag="NBI42_000357"
     CDS             complement(8310..8654)
                     /locus_tag="NBI42_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1776821.1"
                     /translation="MVKQLNSVEAFQAFINQHPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSHVEAIAGELNIFTVPVDLIFMDGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(8695..9105)
                     /locus_tag="NBI42_000358"
     CDS             complement(8695..9105)
                     /locus_tag="NBI42_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD1776822.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTVFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWIFKFEIVSQS
                     GIHCIGTQIGGFYRLSDKKITRVPQVFQDILTTK"
     gene            complement(9485..11551)
                     /locus_tag="NBI42_000359"
     CDS             complement(9485..11551)
                     /locus_tag="NBI42_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCD1776823.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKSAKELSQAAKDPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIVTGVVLSFAWPPIQDGLNGLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTDHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGSTDIYAPGVGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKEHAPSIVTPIIITNLEDKELVIEDVQDVDPGK
                     LIMTVK"
     gene            complement(11848..13587)
                     /locus_tag="NBI42_000360"
     CDS             complement(11848..13587)
                     /locus_tag="NBI42_000360"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCD1776824.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQDADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPNNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(13634..14323)
                     /locus_tag="NBI42_000361"
     CDS             complement(13634..14323)
                     /locus_tag="NBI42_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCD1776825.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(14316..14711)
                     /gene="cidA"
                     /locus_tag="NBI42_000362"
     CDS             complement(14316..14711)
                     /gene="cidA"
                     /locus_tag="NBI42_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HCD1776826.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(14926..15804)
                     /locus_tag="NBI42_000363"
     CDS             complement(14926..15804)
                     /locus_tag="NBI42_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1776827.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            15971..16189
                     /locus_tag="NBI42_000364"
     CDS             15971..16189
                     /locus_tag="NBI42_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCD1776828.1"
                     /translation="MSFYEFMQNFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            16579..17010
                     /locus_tag="NBI42_000365"
     CDS             16579..17010
                     /locus_tag="NBI42_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1776829.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(17150..18430)
                     /locus_tag="NBI42_000366"
     CDS             complement(17150..18430)
                     /locus_tag="NBI42_000366"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCD1776830.1"
                     /translation="MQNLDKNFRHLSREEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGTLPVGLLPNIIVDEKSYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVEDTEKLSADIKTLEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQIDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQERQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKQEPRANTQVAERILQDLRSQQ"
     gene            18684..19850
                     /locus_tag="NBI42_000367"
     CDS             18684..19850
                     /locus_tag="NBI42_000367"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCD1776831.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKNIGMVIVATESAIDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VITHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLLSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVDFDEQQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(20021..20542)
                     /locus_tag="NBI42_000368"
     CDS             complement(20021..20542)
                     /locus_tag="NBI42_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCD1776832.1"
                     /translation="MVYKSYYDSPVGRLELVSDGVSLIAVLFENQQDDGTREENTSLA
                     IFKEATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQIPYGTLTTYGAIAKKVG
                     KLLDKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            20905..23013
                     /gene="clpL"
                     /locus_tag="NBI42_000369"
     CDS             20905..23013
                     /gene="clpL"
                     /locus_tag="NBI42_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HCD1776833.1"
                     /translation="MNNNFFNSDFDSILRRMMQDMQNSNQTSNKKYYINGKEVSPEEL
                     AQFTQQGGNHSAEQSAQAFQQAALRQQGQQSGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISI
                     DISSLEAGTQYRGAFEENIQKLVEGVKSSQNTVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNIMKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAIDKVA
                     TEKRISELEHDKRKAVSAEKYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDANDIERLKNISSRLRSKIIGQDKAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKEALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGTDQEYDDIMQEMKKFFRPEFLNRFNGIVEFLHLDKESLQEIINLLL
                     DDVQVTLDKKGITMNVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDLDIEDNELLVKGKS"
     gene            complement(23080..23250)
                     /locus_tag="NBI42_000370"
     CDS             complement(23080..23250)
                     /locus_tag="NBI42_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCD1776834.1"
                     /translation="MSVILNILIFLAIFGYALYTLVKFFKRSKQGKCGTCEIDNDCCS
                     TEQHTANHFPGK"
     gene            complement(23263..25257)
                     /gene="feoB"
                     /locus_tag="NBI42_000371"
     CDS             complement(23263..25257)
                     /gene="feoB"
                     /locus_tag="NBI42_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCD1776835.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKHLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSIRDQVAEQVEAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTVLKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVTYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(25269..25496)
                     /locus_tag="NBI42_000372"
     CDS             complement(25269..25496)
                     /locus_tag="NBI42_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCD1776836.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(25712..28201)
                     /gene="farE"
                     /locus_tag="NBI42_000373"
     CDS             complement(25712..28201)
                     /gene="farE"
                     /locus_tag="NBI42_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HCD1776837.1"
                     /translation="MAKFLYKMGTFIAKHKWSALIAWIVIVAAIFIPIATNAPKFDND
                     IKMTGLKSLDTNEKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPSITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDDSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTDIGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAVGTAG
                     SAVIFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVIFAVFSALTLLPALISIFHKRI
                     KVNKLQSNFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNEYALMVVIPKHGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDVHGQDDARSIVLDDKTMALYQEVKQQSASSLFLYDALMDYQNKHQL
                     NSKQQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            28344..28892
                     /gene="farR"
                     /locus_tag="NBI42_000374"
     CDS             28344..28892
                     /gene="farR"
                     /locus_tag="NBI42_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HCD1776838.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTISDTINNKEDLREIAEFQ
                     EEDIEFNKVLKNVCIKIMHDDIKNNRDRIDIDSDVPDNLIFYIYDSLIEGFMHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(28965..29132)
                     /locus_tag="NBI42_000375"
     CDS             complement(28965..29132)
                     /locus_tag="NBI42_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776839.1"
                     /translation="MRYIISIMMGIVLAIWSFKQLSQSHLDSGFIFFFIIYVMYISYF
                     NRDKHTKNKKR"
     gene            complement(29244..30788)
                     /gene="pruA"
                     /locus_tag="NBI42_000376"
     CDS             complement(29244..30788)
                     /gene="pruA"
                     /locus_tag="NBI42_000376"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HCD1776840.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKEDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVHEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNNFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(30986..31579)
                     /locus_tag="NBI42_000377"
     CDS             complement(30986..31579)
                     /locus_tag="NBI42_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCD1776841.1"
                     /translation="MTEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVELAIPFDTDYGWNVKLGKNVFVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPNSLAVGNPCKVIREIDNEYPGGQFCGKD"
     gene            31818..32009
                     /gene="cwrA"
                     /locus_tag="NBI42_000378"
     CDS             31818..32009
                     /gene="cwrA"
                     /locus_tag="NBI42_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HCD1776842.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            32249..34657
                     /locus_tag="NBI42_000379"
     CDS             32249..34657
                     /locus_tag="NBI42_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCD1776843.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPSIFMNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNELMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVAPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERAHQIDTIVLDKTGTITNGRPVVT
                     DYHGDDQTLQLLATAENDSEHPLAEAIVNYAKEKQLTLTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            34948..35154
                     /gene="copZ"
                     /locus_tag="NBI42_000380"
     CDS             34948..35154
                     /gene="copZ"
                     /locus_tag="NBI42_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCD1776844.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSAEVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(35244..36242)
                     /locus_tag="NBI42_000381"
     CDS             complement(35244..36242)
                     /locus_tag="NBI42_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCD1776845.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYS
                     PNSIAEFAVNQAINLVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLRVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGLIKGAALDTYEYERKLFPSDQRG
                     KPLNDPLLESLIGREDVILTPRIAFYTEAAVENLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(36265..37419)
                     /locus_tag="NBI42_000382"
     CDS             complement(36265..37419)
                     /locus_tag="NBI42_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1776846.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQQALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKDTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKARRDRFEAMLAKADLPFVHAKGGIYVWLETPSGYDSEQFEQ
                     FLVKEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(37846..39354)
                     /locus_tag="NBI42_000383"
     CDS             complement(37846..39354)
                     /locus_tag="NBI42_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1776847.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNSHVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYKDYIELKQLRYIYDVYFDRDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLIQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVHIELDAEIEKIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPNVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIEQGV"
     gene            complement(39366..40229)
                     /locus_tag="NBI42_000384"
     CDS             complement(39366..40229)
                     /locus_tag="NBI42_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HCD1776848.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPKNQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQIIEKHPLEHHHFQSDRRIMLALQYVAQHNQIAFQ
                     SFYNLIDTVYKDQHFTMFETDTELLGYCYGVAGTVGEVLTPILSDYTTQQTYDVARRL
                     GESLQLINILRDVGEDLENERIYFSKQRLNQYEVDIAEVYHKGIDERYIALWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKKKKADL
                     FHEINSKYHKV"
     gene            complement(40266..41393)
                     /gene="crtQ"
                     /locus_tag="NBI42_000385"
     CDS             complement(40266..41393)
                     /gene="crtQ"
                     /locus_tag="NBI42_000385"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HCD1776849.1"
                     /translation="MKWLSRILTVIVTMSMACGALMFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNSIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRCYEGFSAIFNLMTVVGMNVFSTLDDGQTNQQAFGPVTLTNKTDYYTTDGHKNANQ
                     HIIEGFALGNAYASQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTNPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVGKMLTVYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYQLTKEK"
     gene            complement(41399..42892)
                     /locus_tag="NBI42_000386"
     CDS             complement(41399..42892)
                     /locus_tag="NBI42_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1776850.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSNKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKMDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRCVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLLHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRNKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYHSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREVKNRK"
     gene            complement(42885..43373)
                     /gene="crtO"
                     /locus_tag="NBI42_000387"
     CDS             complement(42885..43373)
                     /gene="crtO"
                     /locus_tag="NBI42_000387"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HCD1776851.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPNKYFRQKHIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNRNIYDQRHLMTINSDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(43548..44327)
                     /locus_tag="NBI42_000388"
     CDS             complement(43548..44327)
                     /locus_tag="NBI42_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1776852.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLEQNNTYYYNNYNTYNNSYNNYSYNNYNNYNHSYQY
                     NNYTNNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTS
                     GQCTYYVFDRVGGKIGSTWGNASNWASAAASSGYTVNNTPKAGAIMQTTQGYYGHVAY
                     VEGVNSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(44688..46499)
                     /locus_tag="NBI42_000389"
     CDS             complement(44688..46499)
                     /locus_tag="NBI42_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1776853.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQAANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKNQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASE
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
BASE COUNT        14821 a         8873 c         7033 g        15797 t
ORIGIN      
        1 cttttcttac ttgggtattt tttaccaata ggtatagaca aagttaaaaa tttaagctat
       61 agccagtttt tcttcagttc atatactgtt gcaacacaat tagggttctt actattttca
      121 tttgttattg catattttat taataaagag tattcgaata aaaatatcct gttttataaa
      181 ttgattggcg ataatatctt taccttcttc tataaaaagg tatcagtgtt attttttgaa
      241 tgtttagtgt tcataattct aagtatcaca attatttcaa ttatatattc gaatttctca
      301 cattacttac tattaataat cttattttca ctcgttatct tacaatatat cttagttgtt
      361 ggaacgatta gtatggtttc acctaacatt ttaatcagct taggtatcag tattgtatat
      421 tggattggtt cagtcatctt agttgccatt aataaaaata tatttggtat cgttgctcca
      481 tttgaggcga gcaatacgat gtatcgtgcc gtagaaaaaa tattgaataa cgagtcaaca
      541 tttatatgtc ctactgaaat tataaataca gtattattct tcgttcttct acttatagtt
      601 aatactattg tcttattact aagtagaaag agatggttga aaatcggtat gtagtacata
      661 aaaaggagcg gcatccattg tcgctccttt ttattatgta tttttacaat ctccaccaat
      721 gacacgtcgt cattgttgcc tcacaccaaa caaaaaaaca gtctcgcaat caagactgtt
      781 ttccactcaa tatattcatc cattatcaca atggattatt tgacttctgt agctacaaag
      841 attttacgtt tttcccaaac gcctgtcttt tcattgtaat catcacaagt aattaatgtt
      901 aattgtttat ctttaccttt ttgttcatct agaacttcca catctgtagg cttaacatct
      961 cttatacttg tcattttata cttgcgtgtt tcattaccaa ctttaaagta caccatacta
     1021 ccttttttgg ctgctttaag atttgtaaat tgatagttcg gacggtcaat gaaagtgtgt
     1081 cctgcaattg aaatattttg atcatctagt gattcatttt cttctgcaaa gcttacacct
     1141 ctatttaatt gttcaggtgt tgctggtcct ggatatactg gttctttaat atcagcatct
     1201 ggaatttcaa tatagcctgc cactttcgat ttatctttcg gaatttgagg tttagcttgc
     1261 tgctttttat ccttactcgc ctgttctttt acatttttat catattgttc aatcttttca
     1321 tctttatctt tatcgtgaag ataattatcg atatgtggtt tagcaaacaa atatgctgcc
     1381 actaggataa gtaccacacc agcgattgtc attaatcgat ttgtccattt tttcatacgt
     1441 taaggctcct tttatacatt tcacaatgcc tattaacatt tcatatagta taccattaat
     1501 ttcaaaatga ctcatagcaa ttcattttat actataaaat ttacatgtat acttttacgt
     1561 tagatttcat tacacatatt tgcattcaaa taacgaaacg cttttaataa ttactaaggg
     1621 ggaattgtta tgattagatg cgctaaaaaa gaagatttaa acgctatatt agcgatatac
     1681 aacgatgcca ttattaatac aacagctgtt tatacttatg aaccgcaaac catagacgaa
     1741 cgtattgcat ggtttgaaac gaaacaacat aatcatgagc ctatctttgt atttgaaagt
     1801 gatggtaatg ttttaggatt tgcaacattt ggttcgttta gaccttggcc agcatatcaa
     1861 tatactatcg agcattctat ttatgtcgat gcttcagcta gaggtaaggg gatcgctagt
     1921 caattattac aacatttaat tgtagaggca aaagctaaag gttatcgtgc gctaattgca
     1981 ggcattgatg cttccaacaa agcgagtatt cagttgcatc aaaagtttgg ttttaagcat
     2041 gccggcacac taaccaatgt aggttttaaa tttaatcaat ggttagattt agcattttac
     2101 gaattagatt tacaagacta gtaatgtttg aatcacataa tatcaacaag acgaccatgt
     2161 caattcactt aacataacaa gccaacagat aaaattttaa acttctcagg gacaatttac
     2221 atcgtccctt ttatttattc ttttcaaata ctatccccat agctttaatt taacgctttt
     2281 tctcaataac aaaacgaata tagtagaaca tgaaaacgat aatcatgctg agtgataaag
     2341 atttaaataa tagattgacc cacgttccct cagtcgtata tccatatgta atcgttgtgt
     2401 taatgatgaa tgctataaag atgattgata gtcttagcat atcatcactc cttttaagtt
     2461 attttagata tacgggggcg cttttgcaat cactattttg attagcatgc atttgccata
     2521 aatttttcaa cttcatcata gataattaag aagcatctat ctggtactaa taatccagac
     2581 agatgcttct ttatatcaat atttcagtgt tatttcatta attattttta accatatctt
     2641 cagctgtgcc aaagatttta cgtttaattg cttcgccagt tggtgtacct gctaatccac
     2701 ccaatccagt ttcacgtaat gatgcaggaa ggttacgacc aaccttatcc attgcttcaa
     2761 taacttcatc aacaggaatt ctactttcaa tacctgctaa tgctaaatct gctgaaatta
     2821 aagcattacc tgaaccaatt gcatttctca taacacaagg aatttcaaca agtccggcta
     2881 ctggatcaca aactaaacct aataaattac ttatcgctaa tgccattgca tgcccggatg
     2941 cttctggtga tcctccgaat atagctactg ctgcagctgc cgccattgca gatgctgaac
     3001 caacttcagc ttggcagcca cctgttgcac cagctacact tgcattgttt gctacgacac
     3061 gcccaaacaa tgctgaagtg aataagaagt caatcatttg ctcttctgtt aaatcatgtg
     3121 ttttttctaa tttaaaaagt gcaccgggaa tggtacccga ggaaccagct gttggcgttg
     3181 cacaaataat acccatcgca gcattgactt catttgttgc aatggcacct ttgactgcat
     3241 caatcatttc atatccagac aaagcatgat gtgtttcatt ataatcacgt agtttagcag
     3301 cgtcatgacc agtgtagccc gttacacttt caacgccatc acctgtcgtc cctttgatta
     3361 ctgcgtctcg catgacatct aaattttgtt tcatttgcgc tcgcacttca tcacgtgatt
     3421 taccgcttaa ttccatttct tctttaacca tgatatccgc aaatgacata ttattttcta
     3481 cggcataatc tatagtctct ctaattgaat caaacatgtt tattccccct ctaatttata
     3541 taggaaacgt ttacgtcact gtatttctct ttaattgtat ttaatgtcga ttctgagatt
     3601 gctttattta atggtattac aaccaagcat ttatcttcat ctattttaat aaattcatct
     3661 ttacagtcta atttcatatc gttgatatca ttaatgaaat gatttacttg tgctttagtc
     3721 atatttccgt caacaactaa aattggtaat ccatgattta aatctacttg tagtccattt
     3781 atatgaatac ctttaatttt aattgtacca ccaccgattg aaataccgat aatttcaatg
     3841 tagcgaccat cattacgaga tgatttaata taagcacagt ttggatgttg accaatacta
     3901 tcgccttctt cttcgataat atctatttta ataccatcat cagctgcaat ttctaatgaa
     3961 gatttaattc ggttatcaaa tgttgaatat cccattgctc cacccacaat agcgacatct
     4021 gtaccatgtc cttggtgtgt ttgagcaaat gattcataat aatgtatttc aatattttta
     4081 ggaatatctc ccaatattgc gcgtgctgaa ttcccaatct ttactgcacc agccgtatga
     4141 gaacttgaag gtcccatcat aactggtccg ataatatcga aagcactttg ataatcatag
     4201 ctctttgcca taattaaaca ctctccttaa tatgattctt tttgctcggc attttaaagt
     4261 tgatattcat taaattaaac ttattaatta gtgttccaat aatataggct aagacaatgc
     4321 tgacaataat cactgttgcg attgttacaa ttgatgttac tgcattatta aagccaaaca
     4381 atacgatggc acctgcaatt ggtgttgcca tacctttgac acctattact agtccgctaa
     4441 atgtcacgat acatgcgttg acgaccccaa tcagtgcatt tgtaccatat agttgtactg
     4501 gatattgcgc tattaaatca atttgcgtca atggctcaat acaaactgca aatgcttttg
     4561 acggtccacc aatatttaat tttcggaata aaataaggtt aacaaatgag ctacctgtac
     4621 atgttagtgc tccaatagcc ataggaacac ctgtaagtcc taataaactt gttaatacca
     4681 ttgaacttag cggcgtcata cctgtaacag gaatcactag tcctaaaatg accgctaatg
     4741 catatggatt attgtcacct actgctgtga cagcactacc tatttgtttt aatgttgcta
     4801 acacaccagg tgtgataatt gatgcaagtc cgaaggcaat tgctggtgca aataagatca
     4861 ccacaattaa gtccaagcct tctggaactt tcttttcaat ccatttaatt aaaaaagcta
     4921 cgccataagc tgcgatgaat gctggtaata atttaaagtc atgtaatact aaaccaacaa
     4981 tgaccgcaaa tactggtgca acgcctaagt ttaagcacgt tagaatacct actgcgatac
     5041 cgcttaaact tcctgctaaa tccccaatat cttgtagaaa tttaatatca aatacgccac
     5101 caatagcgta acttaagaat gcttgtggta gaaatgtcgc acaagctgca ccggataatg
     5161 cttgtagtcc ttgtttaccg tacggtgcat actttaaaaa tagcgtcatg atcactaaaa
     5221 ccaagactaa tgtacctaca cctaacagaa tatccatttc ccaaaaacct ctctctatgt
     5281 ttattttatt ttcagaccat aaacatcgta cacccttaag aaaacgtttt caacttttat
     5341 ctgttatcaa atcaaatatt taagtgaaat atttcatatt tgtgaaagat ttttaaaatg
     5401 gattgtttca aaaactactt atgttgtcat taatatttac taattaactt tttactctat
     5461 atttcaaaca gttgtgtgac agttttttga taactttttt acatctgaaa gtaagtaata
     5521 tttctaaaaa cttttaatat ttatacactt tatctttcga gctatttatt tttgtttttg
     5581 tgctgtgttt ataaaaccgc acactatatt caaaaataac ttattacaca tatatttgca
     5641 ataagataat taaagtagga tattattttt agttttctga taggaatgat gatagtctat
     5701 aggttgaatc tttacttttt ttaaagctaa atttacatca acttaacaat ggctggttat
     5761 actgaagatg aaaatattta gtataactta gtggaggcgg taaaggtgca attgagtcat
     5821 tccgttaaag ttgcaatttc tatctattta gcacttatct ttataacgtt cacttcttat
     5881 ttagtcatta ttttatatac gagtatgact ggacatgatg tatcacattt cgtgttagat
     5941 agtcagcatt ctcatcatgg atctcttacg caaaaacatt tgagtcttcc tgaaatctca
     6001 tttaaatagt ttatcctctg tgtttcaaca ttcatatccc atatcgattc atttatctat
     6061 catctagacc actacatctt agatgatttt tttattttct catttcactc tttcttaaaa
     6121 gtcgatataa tgaattaaat cattatcata cactgacata ttttatgttg ttggtgttaa
     6181 gttttaaagg ggtgagatac ttggtgaata atcattcagc tttacgttta aaaggaatta
     6241 tacttgctat tgtcggtgct tgtttatggg gattaggtgg tactgtttct gatttcttgt
     6301 tcaaatataa gaatattaat gtcgattggt acgttactgc tcgacttgta gtcagtggtg
     6361 ttttcttact tattatgtac aaaatgatgc aacccaaacg ttctatattt agcgtattcc
     6421 aagatcgacg tatgttaggc aaattactta tcttcagtat actgggcatg ttagttgtac
     6481 aatatgctta tatggcatct attaatacag gtaatgctgc gattgcaaca ttactacaat
     6541 acattgcgcc agtttatatt attatttggt ttgtcataag aggcgttgca aaactaacat
     6601 tatttgatgt gcttgctatt atcatgacac tattaggaac atttttatta ttaacgaatg
     6661 gttcattttc taatttagtc gtcaatcctg caagtttatt ctggggtatt ttagctggtg
     6721 tagcactcgc tttttacaca atttatcctt cagacctact taaccgcttc ggttcaattc
     6781 taattgtcgg gtgggcgatg cttatatctg gcattgcaat gaatttacgc catccaattt
     6841 ggcacattga tatcactaaa tgggacatat caattatatt atttttaatc tttggaatta
     6901 tcggcggtac tgcactcgca ttttatttct ttatcgacag tttacaatac atatcagcga
     6961 aagaaacaac attattcgga actgttgaac ctgtcgtagc agtcatcgca agcagtctat
     7021 ggttacacgt ggcattcaaa ccatttcaaa tcgtaggcat cattcttatt atgattttga
     7081 tattattact atcacttaaa agacaaccta aagaaataga tgaataagaa aactctgatt
     7141 atcactttag caagtatcta ctatttaaca acgtagctac attgtaggtg atatcagagt
     7201 tttttatttt agttaataat atttttaatt tggtataaaa agcgtcggcg ctctggtatt
     7261 gggaatactg gataaatatg aaattgataa tatccaggta taaagtcatg ttcgatacct
     7321 tttgcactca acaattgtga taagttcaat gcatcaggat acagcacttc ttttgtacca
     7381 acagttaagg taattcgtcc taaaccatct aagtcaccat taattggtga tactttgtag
     7441 ttatctaaag gtgtgtcccc tgcccattgt tcagctaaga acacactgcc atccacacct
     7501 accattgggt ctttctttaa gtagtcagga atttcaggat gctgcatcgt agcatctaaa
     7561 actggtgaaa ttaatacaat atgtcctggt tgcacaatat gtttttcttt taacaattga
     7621 gcaaatgata aagcaatttg accgcccgca gagtcaccca ttacaacgat ttgtttagaa
     7681 tctgctactt gattcaataa atcatggtac aacttttcaa aaagtacata cgtcgcttga
     7741 taatcttgat gcggaatctt cggatatact ggcatgatga ctttagcatt gagtgtttct
     7801 gcaagttcat caataaattc gaaatgaatt ttgagtgggt cttggaacca tgcgccccca
     7861 tgtgcatata atacaacgcg ttgatgctta tcttgtttat cgttaacggt ataaacggtt
     7921 gaaccgaagt gcttatccat ttttactggc atcttaaact gataattggt gccctcatat
     7981 tcacctgctg tctgtggcgc cattttttct agtgctttct tcgcttcttc tttatacata
     8041 aaacgacgct tagatcttga taatcttata ccttgttcta acatataact tttaacactg
     8101 cgtttttctt taattctaat atgtgcgtat gccgctacta aaaatccaaa cgcaagtgta
     8161 gaccattttt tacgcatttg tcgtcactcc tctttgatgt tttctactat agtattaatg
     8221 taccatgctc attcttatta ctaccaatat ttactgggaa ataagttatg atgtctacat
     8281 aatcttagca atgctaatca tttgcgatat taatgctcat cgacatcgtt attcacacta
     8341 tcattcattt gcttaagatg atgttcaaaa cgttgcatat cgataaatcg gccttggcga
     8401 tgcatttctt ttccatccat aaaaattaaa tcaacaggta ccgtgaaaat atttaactct
     8461 ccagcaatag cttccacatg actttgattt atcacagcta atggcacatt gggatatgat
     8521 tgcatcaagt cttcaatttg tggtaaaacg gcatgacaca cgctacattg gtctctcatg
     8581 acatgtacga ctgctaacgg gtgctgattt ataaatgctt ggaatgcttc gacactattt
     8641 aattgcttaa ccatttcgaa caccttctta cgttttttat ttttaatacg gtcattattt
     8701 ggtagttaaa atgtcttgaa atacttgtgg aacacgtgta attttcttat cgctcaatct
     8761 ataaaaaccg cctatctgtg taccgatgca atgtatacca ctttgagaca caatttcaaa
     8821 cttaaatatc catttcattt tacttgcttt aactaaatat atgcgaccta ctggtttatc
     8881 atgaatcgtt actggtactt tgtattccgc ttcagttttc ataagtatag gtgaaatgcc
     8941 ttgattgatc attgttgagt aattcatatg ctgatttata aaaactgttc tcaaatcttc
     9001 gaaccatcta atataaacga tattacttac gacacccatc gcatcaatgt cgtaaccatt
     9061 tacttcgatt tctttctctg caactaacaa tccttctgtt gtcataatga caaatctccc
     9121 aacattacaa attgatttaa aatcatgtta atttattttt aatactttca tgaaaaaaat
     9181 acaagcaatt gaactttatt acggaaaatc aaacttatca atgacgctat taatttgact
     9241 agtcgcaaag atgtattttt ctaaaatgcg acaaaaacat aagcaatgtt ttctatatct
     9301 aacactaata tttaccttat tctcatcaac acacctgcta tacatcattg cgcactaaaa
     9361 aggcagtagc ttcattttaa gctactgcct tttgtaacgt attctcttcc aaatctctaa
     9421 ttcacgtatg aactacgaat gaatagaact tcattgattt gatatgttgt tgcattttca
     9481 atcattattt gactgtcata attaatttac caggatcaac atcttgtaca tcttcaatga
     9541 caagttcttt atcttcaagg tttgtaataa taattggtgt tacaatgctt ggcgcatgct
     9601 ctttcaagta tgctaaattc actttcatta acggttgacc ttgtgtcact ttttcatcca
     9661 cattaattaa actttcgaat ccttcaccat tcagtttcac tgtatcgata ccaatatgaa
     9721 taagtacttc gacaccactt tcagattcta aacctatcgc atgttttgta gggaagattg
     9781 ttttcactgt accgtcaaac ggcgccacaa tttcaccttt ttcaggaata aagccaacac
     9841 catcacccat cattttacca gcgaatactt gatcaggtac ttctgataat ggaatgattt
     9901 gaccgacacc tggtgcataa atatctgttg aaccaagagc ttctacgtga actggttcat
     9961 ctgacatttc ttcagtcact gtcgtttcac ttggtttcgt aatttcacca ctcataatct
    10021 tcgccatatc atgtttaatt tgatctgatt ttggaccgaa gatagcttgc atattgttac
    10081 ccacttctaa tacacctgaa gcacctaaag ctttaatgcc tgctacatcc acttttgatt
    10141 tatcaaccac ttctacgcgc agacgtgtaa tacatgcatc taaatgttta atattttctt
    10201 ttccacccat cgcatctaag acatcaaatg gtaatttagc aacgctagag ttacgaattt
    10261 cagtttcttc atcttcacga cctggtgttt tcaatttaaa cttacgaatt gcaaagtcga
    10321 ataagaagta atacacaatt gcgtatacaa taccgactgg aataactaat aatgcatgtg
    10381 agcgatccca gtttaataaa ccatataaaa tataatctat gaaaccacct gagaatgtca
    10441 taccaatttt aacgcctaat aaatgcatta ctaagaatga tgtaccagct aataatacgt
    10501 gaattccata aagtactggt gctacaaata agaatgaaaa ttctaatggc tcagtgatac
    10561 cagttaaaaa tgcagttaat cctgctgata acattaaacc accaacaact tttttacgtt
    10621 ctggtcgtgc atttttataa atagcaaatg ctgccgctgg taaaccaaac atcatgaatg
    10681 ggtatttacc agtagtaaat gcaccagcag taaatggtac gccatctttc aattgtgcca
    10741 tccaaatacg ttggtcacca cgaactaatt cacctgcatg atctgtataa cttccgaatt
    10801 caaaccagaa cggtgaatag aaaatatgat gtaaaccaaa tggaattaat gaacgctcaa
    10861 taataccgaa tataaacgtt gttaatgtta agtttttatt taataagaaa ttcgataaac
    10921 catttaatcc gtcttgaatt ggaggccatg caaagcttaa aactacacct gttacaatag
    10981 ctacaaccga agtcacaatc ggaacaaatc gtttacctgc aaagaatcct aaaaatggtg
    11041 gtaacgttat gttataaaac ttgttgtaac accatgctgc taaagcaccc attataatac
    11101 caccaaatac acccgtttgt aatgttggaa tccctaatac taaagcatgt gctggatctt
    11161 tcgctgcttg acttaattct tttgcacttt tggcatatga gaaaatatca tcaattgtaa
    11221 tatgcaacac ttttcccatt gttgcattca taattaagta acctactagc gctgctaatg
    11281 ctgcaacacc gtctcctcct gctaatccaa gtgctgtacc aactgcaaat aatagtggca
    11341 agttatcaaa tacaacttgt cctgcagctt ccatgaccga cgaaatcatt acaatgatat
    11401 cgttttttaa ccatggtgca atttctacta attgttcgtt gtgcattgcg ttaccaaacg
    11461 ctaataaaat accagctgct ggtaaaatcg caacaggtaa cattaatgct ttaccgatac
    11521 gttgcaattg tccaaacaat ttcttaaaca tataaacatc ccctatctca atttccgctt
    11581 ccatgtacaa aaaagcaggc atgaacaaac aagactattc tcatagtacg gttgtttgct
    11641 catgcctgca ttaccagtaa cacgcaagtg atatgtaatt catagctatt atacatattt
    11701 ataaaagcgt tttcaacaac ttttttaaaa tattataaac actataatac gacgctttat
    11761 gaaaacaaat aaaacggtaa cttctattta attcaaacag aaattaccgt ttatagctaa
    11821 attgaattct gaaagccaat caacagatta taagaaacgt tttgctgcaa ctgaaatagg
    11881 tggaatttga tctaaatcta aatgtttatc ttcagtaata gatctaaatg cccatttacc
    11941 ataaccaagc gcttcttcat ttacaatttt acctggtaat ggcgcagcat taggatcaac
    12001 ataaacatct acaattgttg gtacatcttg tgctagtgcc tcttctacaa tagcatctac
    12061 ttcgctagca cttttaattg tataaccttt accacctgct gcttcagcaa atttggcatg
    12121 atccatatca gagaaatcaa ccgcatattc taattcacca gcagcttgtt gttcatattt
    12181 aataaacgct aattgcttat tatttagaac aaacactgtt aaaggtaaat catattgtac
    12241 agctgttgca aagtcctgca tcaccatttg gaatgcaccg tcaccagcaa ttgcgatggc
    12301 ttgtctattt ggatatgcaa tttttgatgc cattgcacct ggaagaccgc aacccattgt
    12361 acctaaccaa cttgaaatga tgaacttgtt atttacacca aggtttaagt atcgagttga
    12421 ccaaactgtt gctgtaccta catctgctga aatcactgca tcatctttaa taaatttatt
    12481 gattgatgcc attaatcgtt ctggacgtaa tggtttacta ttattatttt tatcttgttc
    12541 catccattta tcccaaaccg ctttacgttc taacgttttg tttaagaatg gtctttcagc
    12601 aacatgttta atattttcag ttaactgatg caacgcaatt ttactatctc caacaattcc
    12661 tacattaata ttaaaacgat gtccgatatt gttaggattt gtgtcaattt gaatggcttt
    12721 aatatttttc ttaggtaagt aatccacata tggatagttt gtaccaacca taattagtaa
    12781 gtcagcatcc tgcatcgttt gatatgatgt tttcgttcca attttaccta agttaccgat
    12841 actatatgga tgatcatccg gcaagattgt tttagctggt aatgaatgaa tgacaggaat
    12901 tttagccatt tcaataaatt cacgtagctc atctttcgca tgtttcgcac ctacaccaat
    12961 taacatgaca ggctttttac ttttattaat tagtttaacc gcttttttga tgtctttata
    13021 ttttggtgat acaactgttg gtcttgatgt atctactggt ttattcgttg tatctttaat
    13081 tttttcagtt aataagtcgt taggacaaat aacaacagcg acaccttttt gttcatatgc
    13141 cgtacgaatt gcttcgttaa cgatttcaaa cacattatca cctttttcaa tttggtgatt
    13201 ataaacggct acatcttcac ataatttttg taaatttgtt tcttggaatg ctttcgttcc
    13261 aagtgctgta ctattcgttt gcccagataa aattaattgt ggtacgttat ccattttggc
    13321 atcatacata ccattcaata aatggattaa accaggtcca ccaatactta atgcaacacc
    13381 gattttacct gtaagtttcg tataacccgc agcagctaaa cttgctactt cttcatgtcg
    13441 tacatgataa aacttaaatt gatctctcac tgtacgtaaa ctatcaacaa ctgcgtcgat
    13501 tgagtctccc ggaataccat ataagtgatc tatatcccat gcttgtaatg cttttactaa
    13561 tgcttcattt gctttaattt ttgccattat tactaatagc ctccctttct gtttatcatt
    13621 ttaaatttct aaattaatag aataataaaa ttagaacagg tattaacact gaactaataa
    13681 ctgcagttaa aatcatccca attgaactaa atgcaccaga ttctatatcc atttccagcg
    13741 ctttagctgt accaaaggca tgtgacgcat tcccatacgt taaaccttta gcaatagaag
    13801 attcaaatct tccaaatctt aataacatcg atcctaaaat acttccaatt aaaccagttg
    13861 tgattataaa aagtacggtc atcgtatctg taccacctaa ttcatgtgac acttcaatac
    13921 cgacagcggc tgtgattgag cggggcaata atgttactat gacatcttta gaataaccaa
    13981 acgctttaag tgttaagaac actaacatga aattcagcat gacgccagtt aatacacttg
    14041 caaaaatgat agagacattg tctttaattt tctctctatt tttataaagt gggtatgcta
    14101 aacatacgac cgttgcgttt aaaatatggt tgatccaact gccacctttc atatacccgt
    14161 tataactaat tccaaagata agtaagacaa aaataattcc taaagaagca attaatgctg
    14221 gattcaaaaa tgggttcgga tatttttgtt gtaacctttt agcgaaataa tataaaacga
    14281 cagtcaacaa aatcattaat aaggcttgca cgtaatcatt cataagcgtc tacaccttta
    14341 cgatgtttat gtttaacaga cattttttca gcaatataac ctgaagataa tgcaacgata
    14401 catgttccta tgataatgac tgcgaaaaag agtatataat ttagcgtaat ttcggaagca
    14461 acatccataa tacctacaac tgacggtatg aagaaaaaga ccatcgtctt taataaaaag
    14521 tttgcaccgt cttctaccca agttagcggt acaatcttaa attgtaatag taaataaaat
    14581 aaaaatagac caacaatgct gccggctaaa ggaagatgga aaatcttttg aatttctgtg
    14641 ccaatataag taatcactat gatgattcct agttgtaata acagtttgat tattaattgg
    14701 actttgtgca tggcgccatc cctttctaaa tatgtctaaa ttgttacaat aactattata
    14761 aagatggcgt acacaatata aaaatactct ttttttatga tttatattac tattttctat
    14821 ggaaacgctc tctaaatttt acttcatttt catattaaaa aacactcatc atttcaatga
    14881 cgcacataat gatgcgtctt aaataatgag tgctctatta atatgttacc ctaaacgatc
    14941 tttcaaaaat tcaatccatt tcgttgtggc atgacttaat tgtttatcct tcttccaaac
    15001 aacacctaat tcccaatgta catgtgcatc ttcaatacgt aacagtttca cgtctccgtt
    15061 caactgttct gatatcgatg ttggtaatat actaattcct aattcatttg tgactaaatc
    15121 ttcaataaca tgccattgcg aaatttgcgc tacagtatta ggtacaaaac ctacgttttt
    15181 agcattctct ataattttat cgtttaaata aaaatcttta ttgaataaaa tgaagtcttc
    15241 acctgctaaa tcttcgagtt taacggtttc atattttgcg agtcgatgct ctctgctcac
    15301 gataagtcgc aaatcttcct tatctagggt agtataatcg aaaatatgat gatcgactgg
    15361 caaagtggtc acgcctatat ctacttcatc attaataatt tgctgctcaa ttgttttgcc
    15421 accattttct atcaaattat aagtaacatt tgggtactgc tgatggaacg caccaagaat
    15481 attaataaga atcttcatat tcatgaccgc ggacattccc atattaatat gtcctgtttc
    15541 cagtccattc aagcgttcca tttcagatgg taaataatca tacagtgcaa caatttcttt
    15601 acttttctca taaaaaattt gacctgcatc ggtaagtatt aaatgtcttt tacttctatc
    15661 aaataagggc gtgcccattt cattttcaat atctttaatt gctttactaa ttgtaggttg
    15721 tgcaatatat aatgatttgg aagcattagt catgcctcct tgcttaacga cttcaataaa
    15781 atatttcata tgtttgatat ccactaacat catcctaact atttcaacac gtttatttca
    15841 ttatacacaa cttcttaaac attcaataaa tttagattta taaaattgct ttataaaaag
    15901 tttcatgtaa aatacacatt cttttatgtt attataataa tggctcaagt atattaatcg
    15961 aggtgaggta ttgagctttt acgaatttat gcaaaatttt cttggtgatg acacaccatt
    16021 aggtgaattg gttgattgga ttaatcaaga tagcaatttc cctagagaag tgaagagcca
    16081 tagtgaaata ttgtcatatt ttcgaacaaa tccatgtcct gaaagcatct cagtacctgt
    16141 aattaaacgg gcactatcag ttttcaacca attcacaaat gtacaatgat acatgaatac
    16201 atggagtcac atcatctcat gttagcgctc tgattgcagg gcgctatttt taatgttatc
    16261 gcttcattta ttatgttgtg attgctattt tctttcagtt atagaaccag gtatcttttc
    16321 ttttaaatca ttttatattt tcccactaat atccgctgtt aatcaatcct gacatccttg
    16381 tatcactatg acacttaatt gttagataca tgaatttcta cattttatga aaaaatccat
    16441 ttttattaca atttaacact ttatatgaca acttcattac agttactttt attgttgatt
    16501 gcttgcattg ttttctaaaa aaattttgtt atcataaata acgttgaata aagaaaaaaa
    16561 ttaacttggg agataaaaat ggaatataaa aagatactaa ttcgtttatt aattgctttt
    16621 gcagtacttt tctcagcaga tttcacttat caatccgtcg aacaaacgca tcaatcgcat
    16681 gccgcagtta attattatag taaaaaccaa tgtacatggt gggcatttaa acgtcgcgca
    16741 caagtcggca aacctgtttc taatagatgg ggcaatgcta aaaattggta ttacaatgca
    16801 cgtaaatcaa aatatgcgac tggtcgtaca ccaagaaaat ttgctgtcat gcaatcaact
    16861 gcaggatatt atggacatgt cgcagttgtt gaacaagtat ataaaaacgg tagtattaaa
    16921 gtttcagaat acaactttta tcgcccatta aaatacaata cacgtgtact aagcaaaaag
    16981 gcagcacgta actttaacta tatttactaa tcaaaaaact tctatcacga acgcttcaaa
    17041 tttctctatg cgtgtgatag aagtttttta ttttttgttc atattaattt atttaagcgc
    17101 tacgatggac tgacttaaac ttttctttaa aatcgctatt cgcaatttac tattgttggc
    17161 ttcttaaatc ttgtaaaatg cgttcagcta cttgtgtatt cgcacggggt tcttgcttca
    17221 atgcttcagc tactttagca atttcatcac cttttgcccc tacaacgata gctaatgatt
    17281 tatattgtaa actcatgtga ccttgttgaa taccttctga cacaagtgca cgacatgctg
    17341 caaagttttg agctaatcct accgccgcaa ctacatgccc taattcttgc gctgactcta
    17401 catttagtag ctctaatgat gctttagcta ttggcaatac tttcgttcca ccgccaacaa
    17461 ttgctaatgt catcggcact tcaattgtac caattaaacg ttgacgttct tgatcgtaac
    17521 gccatgtagc aataccacga tactgtccgt cacgactcgc atatgcatgc gcacttgctt
    17581 ctgcaccacg cgtatcattt cctgttgcta aaacaacggc atgtatgcca ttcataacac
    17641 ctttattatg tgttgcagca cgatgaatat caatttgtgc caatacagaa gcacgttcca
    17701 ttcgtttggc aacctcttct ccagttctct cgccccttgc taaatcttta acgtcaattt
    17761 cgccttgaac tttaacaacg gacgctgttg catgatttga taaaatactc attaaaatgt
    17821 cgctttgcgg aaattcattt tttaaaaatg cagttatggc ctctaaaatc gtattaagca
    17881 tattagcgcc catcgcatct tttgtatcaa caaatacttt caaagatagt aattgttgtt
    17941 cagggaatgt atcaatagct atacgttggt aaccaccacc acgcgcttta atagaaggat
    18001 atgcctcatc tgcaatttta tgaatttgct tttctaaagt tttaatgtct gctgataatt
    18061 tttcagtatc ctcgacacca tcaaagacga tttgacctat cataatacgc tctgaagata
    18121 cagttttaaa tccgccagtc tgattaacta atttagcgcc ataacttgca gcggcaacaa
    18181 ctgaaggctc ttccaccatc ataggcacaa cataagactt ttcgtctaca ataatatttg
    18241 gcagtaaccc aactggcaac gtaccttgtg caataacatt ttcaattaaa ctattggcta
    18301 cttcttcatc gattaatggg tgattcagta aaatgtcgaa ttgttcttct gataaccatt
    18361 gcttatcaac caattgttgt aacttttctt cacgagataa atgtcgaaaa ttcttatcta
    18421 aattttgcat ggacatactc cttttacttc acataatttt taacatttca atcactacta
    18481 tttttaccac aaaataacgt cattcgtctt aaaattcaat tgaataattg tcgttttgac
    18541 tttaaaataa aacaaggtaa attaaaacgc ttacaagaaa cgacaaatca tttttaaatt
    18601 tagtatattt ctttgtaaaa aattagcata ttctgatatg atacaagtgt tgcttttata
    18661 aatttgaaag gatgtaaaac cttatgacaa taggtatcga taaaataaac ttttacgttc
    18721 caaagtacta tgtagacatg gctaaattag cagaagcacg ccaagtagat ccaaacaaat
    18781 ttttaattgg tattggtcaa actgaaatgg ctgttagtcc tgtaaaccaa gacatcgttt
    18841 caatgggcgc taacgctgct aaggacatta taacagacga agacaaaaag aatattggta
    18901 tggtaatcgt ggcaactgaa tcagcaattg atgctgctaa agcagccgct gttcaaattc
    18961 acaacttatt aggtattcaa ccttttgcac gttgctttga aatgaaagaa gcttgttatg
    19021 ctgcaacacc agcaattcaa ttagctaaag attatttagc gacaagacct aatgaaaaag
    19081 tattagttat tgctacagat acagcacgtt atggattaaa ttcaggtggt gaaccaacac
    19141 aaggtgctgg tgcagtcgcg atggtaatta cacataatcc aagcatttta gctttaaatg
    19201 aagatgctgt tgcttacact gaagacgttt atgatttctg gcgtccaact ggccataaat
    19261 atccattagt tgatggcgct ttatctaaag atgcttatat ccgttcattc caacaaagct
    19321 ggaatgaata cgcaaaacgt caaggtaagt cgctcgctga cttcgcatca ctatgcttcc
    19381 atgttccatt tacaaaaatg ggtaaaaagg cattagagtc aatcatcgat aacgctgatg
    19441 aaaccactca agagcgttta ctatcaggtt acgaagacgc tgtagattat aaccgttatg
    19501 tcggcaatat ttacactgga tcattgtatt taagtttaat ttcattacta gaaaaccgtg
    19561 atttacaagc tggagaaaca attggtttat tcagttatgg ttcaggttca gttggtgagt
    19621 tttatagtgc gacattagtt gaaggctaca aagatcattt agatcaggct gcgcacaaag
    19681 cattattaaa taaccgtact gaagtatctg ttgatgcata tgaaacattc ttcaaacgtt
    19741 ttgatgacgt agactttgac gaacaacaag atgctgttca tgaagatcgt catattttct
    19801 acttatcaaa tattgaaaat aacgttcgcg aatatcacag accagagtaa tctgtgtatt
    19861 tgaaagacat ttaaatataa cctaaaaaca gcagtaagac agcttctcat tgaaatcgtc
    19921 ttattgctgt tttctattta taatgcttca tcttgaatga attcattatg cctatttgcc
    19981 atattattga agttttccta atgcctggat catttataat ttacggcttc gtgctatgtt
    20041 ttggtacata aagctttgac atatcgatat tctccaactc taacagctta attttattat
    20101 taatcgttcc accgaatcct gttaagctac ccgttttacc gacaacgcga tgacatggca
    20161 cgataattga taatggattg cttccaactg cacctccaac cgcttgggct gacatttttg
    20221 gcttatcaag cagcttgcct acttttttgg caatagcacc atacgttgtt aacgtcccat
    20281 aaggaatttg ccttaattca ttccaaacac actgttgaaa atgactacct gttggtttta
    20341 aaggtattgt gatttcagga ttgtcacctt taaaatacgc gtctaaccac tgtgtcgcct
    20401 ctttaaatat cgctaaagac gtattttctt ccctagtacc atcatcttgt tgattttcaa
    20461 acaaaacagc aatcagactt accccatcac tcacaagttc caatcgtcct acaggcgaat
    20521 catagtaact cttatatacc ataaacacac actccttttg catcatcata ctattcattt
    20581 aactttatga aaatgattaa cactttatgc tttatcaaga tcaactattc acttcaaaat
    20641 caaaatttac atagactacc aacacaatat accatttctt aattaactat gtatcaacac
    20701 atacatttct taaccattga ttaatattta cttttaaatt cttacttact tcaaaaatta
    20761 cacaaaaagt tcataaagtg cctacaatgc gccgttttta aaactacaat ggtggaaaaa
    20821 tagaattgcc attgtttata gtttgcatat agtttcaatg tattttcttt tacttattag
    20881 tttaaaaatt tagaggtgat gtttatgaat aacaattttt tcaatagtga cttcgattct
    20941 attttacgaa gaatgatgca agacatgcaa aactcaaatc agactagtaa caagaaatac
    21001 tatattaatg gtaaagaagt ttcaccggaa gaattagcac aatttacgca acaaggtggt
    21061 aatcattctg ccgaacaaag tgcgcaagct tttcaacaag cagcactaag acaacaaggt
    21121 caacaaagtg gcaatggcaa ttatttagaa caaattggtc gtaacttaac gcaagaggct
    21181 cgtgacggat tattagatcc agtcatcggt cgtgataaag aaatccaaga aactgcagaa
    21241 gtcttaagta gacgcactaa aaacaatcct atcctagtcg gagaagctgg agttggtaaa
    21301 actgcgattg tcgaaggttt agcgcaagca atcgttgaag ggaacgtacc tgcagcaatc
    21361 aaagataaag aaattatatc tatagatatt tcgtcgcttg aagctggaac gcaatatcgt
    21421 ggcgcctttg aagaaaacat ccaaaagtta gttgaaggcg ttaaatcatc acaaaatact
    21481 gtgctcttct ttgacgaaat tcaccaaatc atcggttccg gcgcaacagg aagtgattcc
    21541 ggtagtaaag ggttatctga tattttaaaa ccagcattaa gtcgtggtga aatttcaatt
    21601 ataggtgcaa cgactcaaga tgaatatcga aacaatatca tgaaagacgc tgcattaaca
    21661 cgtcgtttta acgaggtgct ggttaacgaa ccaagcgcaa aagatacggt tgaaatttta
    21721 aaaggtattc gcgaaaaatt cgaagaacac catcaagtca aattaccaga tgacgtatta
    21781 aaggcatgtg tagacttatc cattcaatat attccacaac gcttattacc tgacaaagca
    21841 atcgatgtat tagatattac agctgcacat ttatctgcac aaagccctgc tattgataaa
    21901 gtcgcgactg aaaaacgtat ttctgaattg gaacatgata aacgtaaagc tgtaagtgct
    21961 gaaaaatata aaaaagcaga tgacattcaa aatgaaatca aatctctaca agataaatta
    22021 gaaaacagta atggtgaaca tactgcagta gcaacggtgc atgatatttc tgataccatt
    22081 caacgattaa ctggtattcc tgtatctcaa atggatgcca atgacattga acgtttaaaa
    22141 aatatttcta gtcgtttaag aagtaaaatc atcggtcaag ataaagctgt agaaatggtt
    22201 tctcgtgcca ttcgtcgtaa tcgagctggt tttgatgatg gcaatcgtcc aattggtagc
    22261 ttcctatttg tcggtcctac tggtgttggt aaaacagagc ttgccaaaca gttagccatt
    22321 gatttatttg gtaataaaga agcactgatt cgacttgata tgagtgaata tagtgatact
    22381 actgctgttt ctaaaatgat tggtacaact gctggttatg tcggttatga cgataattca
    22441 aatacattaa ctgaaaaagt acgacgcaat ccatattcag tcattctatt tgatgaaatt
    22501 gaaaaggcta atccacaaat attaacatta ttactacaag tgatggatga tggtaattta
    22561 acagatggtc aaggtaacgt catcaacttt aaaaacacaa tcattatttg tacgtcaaat
    22621 gctggatttg gtaacggtac agaccaagaa tacgatgaca ttatgcaaga aatgaaaaaa
    22681 ttcttccgcc ctgaatttct taaccgcttc aatggtattg tggaattctt acacctagat
    22741 aaagaatcac tacaggaaat cattaattta ttgttggatg atgtacaagt tacattagat
    22801 aaaaaaggta ttacgatgaa tgtctctcaa gatgcaaaag attggttaat tgaagaggga
    22861 tatgatgaag aattaggtgc gcgtccatta agacgcattg ttgaacaaca agtgcgtgac
    22921 aaaataacag attattattt agatcataca gacgttaaac atgtggattt agatatcgaa
    22981 gacaacgaat tactcgtcaa aggtaaatca tgatacttta acatatcaca tatcaaaaaa
    23041 tgagcatcag gacgccctcg cctgtgctca ttttttaaat tatttcccag gaaaatgatt
    23101 cgctgtgtgt tgttctgtac tacaacaatc gttgtctatt tcacaagtgc cacattttcc
    23161 ttgtttcgaa cgtttgaaaa atttcactag tgtatacaag gcatatccga aaattgctaa
    23221 aaaaattaaa atgttaagaa tgactgacac tttaaccact ccttaaacaa ataaatgtcc
    23281 gacttgataa aaaatgaatg ttaagacata tgcagtgact agaggatagg taactgcaag
    23341 tgccgtccat ttccatgaat aagtctcttt acggattgct gccaccgtag aaacacaagg
    23401 aatatacaat agtataaata tcataaatgc atacgcagat agcggtgtga attgattttg
    23461 aatcacatta acaaggcctg catcacctga tgaatagata atcgccatcg aacttacgat
    23521 aacttctttt gctaaaaatc ctggcactaa cgtagcacct gcttgccatg ttccaaatcc
    23581 gagcggttgc actaacatac caaagaagct accaaccata tgtaaaaaac tttgattgat
    23641 attcacattg ataccatgtg gccctacata acttaatagc caaatgacta ctgagccgcc
    23701 aaaaataaat gtacctgctt tacgaacaaa gcccttagcc ttttcccaag tactacgcca
    23761 caacgtttta atggaaggca cacggtatgt tggcaattcc acaataaaga ttgcattatc
    23821 attttttaaa actgtcttag taagtactgt actgactaaa aatgccataa taatacctaa
    23881 aacatacaag cttaatacta ctaaagattg attctctttg aaaaagatgc ctacgaacaa
    23941 tgcatacact ggcagtctag cagagcatga catgaatggt gcaattaata tcgttgttaa
    24001 acgctctttt tcattttcaa tactgcgcgc agccataata ctcggtacgt tacaaccaaa
    24061 tccgataatc atcggtataa aagacttccc gcttaaaccg aacgattcca taatacgatc
    24121 cattaaaacc gcaatacgtg ccatataacc tgaatcttct aataaagata taaagaaaaa
    24181 gagcacaaca atttgtggta caaagactaa tactgatcct acaccagcaa taatgccatc
    24241 tgtaattaaa tcttgtaaaa atggtataac gccaagataa ttcataatcg tcttcacact
    24301 atccgtaaat gtaccaccta taaatgcatc gagttgatcc gacaaaggtg tgccaatcca
    24361 tgtaaatgta gtttgaaaga ttaaccacat aatcgctaga aagataggca tccctatata
    24421 tttatgtgtt aatatcttgt ctattctaga gctaaaatat tgcttatctt catctggata
    24481 cgttaccacg tcttgcaata acgtctcaat ataatgattg cgtatacgct ccatctctcg
    24541 acgaacagat acggcctcta cttgttcggc aacttgatca cgtatactcg acaatttatt
    24601 tacaacctct gaattaagtt cgtttgcaat ttgcatgtta tttaataaga attgaatcgc
    24661 aataaatcta gcttgatact tatcatgaga tgtctctgtc attattattt gacacatatt
    24721 tttaattgtc tcttcaatct tctcaccata attgatttta aaatgcggtt gatacccttc
    24781 ccctagatgc tttatttcgc caagtaaatg tttagttcct ttgcctgtac gtgccacaac
    24841 tggaaaaata ggtgttttta actttttcat caatttatga taatcgattt ttattccgcg
    24901 ctttgtagct acatcaatca tatttaatcc gatgtatatt ggttgattaa gttctaacaa
    24961 ctgtactgtt aattgcatat ttctttttag ttgactcgca tcaacaatgt taatgattcc
    25021 tgaaaatgaa tcgtttaata aatagtctgt cactacagtt tcatctttag aaatcggcga
    25081 taaatcatat gtacctggta aatcaattaa ttgtcctaca ttttctttta gcttccctac
    25141 ttttttctct accgttacgc cactccagtt gcctatgtat tcatacgaac cagttaaagc
    25201 gttaaacaaa gatgttttac caacattagg atttcctaaa atacaataat tttccattcg
    25261 tccggctcct attcttctaa tgcaatagaa caagcatcgc aatgtctaat acttaactgt
    25321 tgtccgttta cttcaataat acatggccct ttaaataaac atttttgttt aatcgttatg
    25381 atagcgtcat ctgttaaccc aaaggcactt agacgataca acatattctc attagcaata
    25441 tccattcgct ttattttata agccttattc atttcaccat ttttaatatt taacatacta
    25501 tttcgctctc ctattagaaa taataatcat tatcacttaa aaatcataac ctttaaaatt
    25561 gtagctcgca atactttatt taaataattt tcatttttaa tgtaaaattt gtgacattgc
    25621 aaaaatattt tgtataatcg cttccatgca aaaaacctcc gcttcaaatg tcattgatag
    25681 acatttaaag cggaggcatg tacatgttat tttatgattt atttcttaag tttttttcta
    25741 acaattgatt gagcttttca atatttttat tcaactgttc tatgttcgtt gcttgttgtt
    25801 ttgaatttag ttgatgcttg ttttgataat ccattaaagc atcatataag aacaatgaac
    25861 ttgctgattg ctgtttcact tcttggtaca atgccatcgt tttatcatct agtactattg
    25921 aacgtgcatc atcttgtcca tgtacatctt gatgtttcga tacatttgtt gcaccttgtg
    25981 gatatacttt aaaggttgtg tcgtatgttt ccttactatc tttagcttca gattcctgtg
    26041 attcaaccgt tttatatttt tcaagtgcat gcccttcaat atcaactcgt ggaataatgc
    26101 gattcaacca tgctggtaaa taccacgaac cttttccaaa caatttagtt aatgcaggaa
    26161 ttaacatcat tctgactacg aaggcatcaa agagtacacc aaacgctaat gccataccca
    26221 ttgatttaat catgacatct tcttggaata caaacgcaaa gaagacacta aacatgatta
    26281 atgcagctgc tactataaca ggaccgcttt ctttcaatcc tactttgata gaataatcat
    26341 tatcccctgt tttactatat tcttcatgaa ttcgcgacat aaggaagact tcgtaatcca
    26401 ttgctaagcc gaatagtata ccaattgtaa taaccggcaa gaatgctagc attggtcctg
    26461 tcgtttcaat accaaacaga cctttcataa agccatcttg cattactaat gttgtaaaac
    26521 ctagcgttgc cattaatgat aagacaaacc ctagtactgc ttttaaaggt attaagattg
    26581 aacggaaaac tatcattaat aagaaaaatg ctaatacaac aatgactgag gcaaataaag
    26641 gtatcgcctc atttaacttt ttggacatat caatattaat tacactttgt cctgaaattt
    26701 ctgtcttaaa tccatactta tcttgcgcat ctttatgata atctcgcaaa tcatgtacta
    26761 agtcatttgt actttctgca ttaggtccat gtttaggtat caccaccatc aatgcatatt
    26821 cattatcttt actcatttgt ggcggagtaa ctatatccac atttttctta tctttaatat
    26881 ctttatacac agattgtaaa tcttgttgta acccttgtgg atcatctttt ttatctttaa
    26941 cgttaatcag catcggtatt tggccattaa atccttcacc gaacttatct gaaataatat
    27001 cgtaagcctt tttctgagta gaatctgctg gctttacgcc atcatccgga atgcctagac
    27061 gcatatgact aactggaata gccgctgcaa ctaatataat taaacctaat aatactgctg
    27121 caagtgcatt accagtaata aatttagacc acggcgtatc aatatctttt ttaaaattag
    27181 actgcaattt gttcacttta atgcgtttat ggaaaatgct aattaacgca ggtaataaag
    27241 tcagtgcact gaatacagca aatataacac taattgccga agcaaacccc atcactgcta
    27301 agaaatctat gccaactagc gataaaccac atacggcaat cacaactgtc acaccagcga
    27361 agatgactgc gctacctgct gttcctactg caagaccaat gcctttaatg taatctgttt
    27421 cagttttcat aacttgtcga tatctgaata aaataaataa agcataatcg ataccaactg
    27481 ctagtccaat cattacggct aatgtcagtg tgacatttgg tatatcgaat gcataagtta
    27541 acaaactgat aatacctaca ccagaagcta aaccaattaa tgcacttata attggtaatc
    27601 ctgcagcaat aactgaacca aatgtgatta acagtacaac aaatgcaaca ataataccaa
    27661 ctaattcaga attaccacct atatctgtag atgtcattcc tgtaccagtt aactctgttt
    27721 gtacattatg attgtcacgt aagtctttca aatgactttt aacattatct ctagagtcat
    27781 cttttaaaga tgtttgacta acgtcatatg tgatatctgc aaatgcagtt gttttatctt
    27841 tactaatttg cttattttca taaggatctg atattttatc aatgtgcttg tcatcttttt
    27901 taatatcatc taacgttttc ttaatatctt ttgtaatact cggttgtaca atcccatcat
    27961 ctttagtcgt cttaaagacc acacgaattt gtgccttttc actatcttga ttaaaatgct
    28021 tttctatttt ttcatttgta tctaatgact ttaatccagt cattttaata tcattgtcaa
    28081 attttggtgc attcgtagca attggtataa atatcgctgc tacaataact atccatgcga
    28141 tgagtgcaga ccatttgtgc tttgcaatga atgtccccat cttatataaa aattttgcca
    28201 aagtattgcc tccttttaaa atcaacgtta tagtttaact atacagtgta gattattgtt
    28261 agattatatt atctatccct tacctcttaa agaattaaat tttaaaattt gtatactaaa
    28321 ctacacatta aggagaaatg tagatgaaag agacagattt aagagttatt aagacgaaaa
    28381 aagcattatc tagtagttta cttcaattgt tagagcagca attattccaa acgattactg
    28441 tcaatcaaat ttgtgacaat gcactcgtac accgcacaac attttataaa cacttttatg
    28501 ataaatacga tcttctagaa tatttgttca accaattgac taaagactac tttgctagag
    28561 atatcagtga ccgtcttaac catccattcc aaacaattag tgatacgatt aataataaag
    28621 aggatttgag agaaatcgca gaattccaag aagaagatat cgaatttaat aaagttctga
    28681 aaaatgtttg cattaaaatt atgcatgatg atatcaaaaa caatagagat cggattgata
    28741 ttgatagtga cgttccagat aatctcatat tttatattta tgattcgttg attgaaggat
    28801 ttatgcattg gattaaagat gaaaaaatag actggcctgg cgaagatatt gataacattt
    28861 tccatagatt aatcaatatt aaaatcaaat agtaggtcaa taacatatat tcatttacct
    28921 taaataacca aaaagataac atgcacgtta tagtacgtgt catgctatct ttttttgttt
    28981 ttagtatgtt tatctctatt aaaataactg atatacatca cataaatgat gaagaaaaaa
    29041 ataaatccgc tatctaaatg actttgactc agttgcttaa atgaccaaat tgctaataca
    29101 attcccatca ttattgaaat aatgtatctc acattcttat acctattatc cttttctaaa
    29161 attatggttg ctattactta atttttaaag ttataaataa aaagagccaa ccacaatgga
    29221 tggcccttgt tcattatgaa gcattagaac atttctgaaa caaccttttg ctctaagaaa
    29281 tgtaataagt agtctggact acctgtttta gcgtctgtac ctgacatctt gaatccacca
    29341 aatggatgat agccaacaac tgctgatgta caacctctat taagatataa attacctaca
    29401 tcaaattcat taactgcttt aatccaatgc tcgcgattat ttgtaatcac tgcaccagtt
    29461 aaaccgtaat ctgtatcatt tgcaacctca attgcttcat caaagttatt aactttcaca
    29521 aagccaacta ccggtccaaa aatttcttcc tgcataattc tatctttaga tttaagtcct
    29581 gaaatgattg tcggttctac aaagtaacct tttgaatcat cagtaccgcc accttgttct
    29641 aatttacctt cttctttacc gatttcaata tagtttttaa ttttatcaaa ttgttttttg
    29701 ttaatcactg gtcccatgta cgtattgtct acagtattgc ccaacgttaa ttcttttgtt
    29761 aatttgattg atttctctaa tacttcgtca taaacatctt tatgcacgat tgcacgtgaa
    29821 catgcagaac atttttgacc tgagaaaccg aatgctgatg taacaatggc ttctgctgcc
    29881 atatcagtat caatattttc atcgactaca atcgcatctt taccacccat ttcagcaatc
    29941 acacgtttca agaagttttg accttcatga acaacagcac tacgttcata aattctagta
    30001 cctgtcgcac gtgatcctgt aaatgtaacg aagtgtgtat ctttatgatc tactaaataa
    30061 tcaccaattt cttttggatc accaggaaca aagttaacga caccttttgg taatcctgct
    30121 tcttctaaaa tttccattaa tttataagcg atataaggtg tatcctcagc aggtttcaat
    30181 aacactgtat tacctgcaac tactggtgct aaagtcgtac cagccataat cgcaaacggg
    30241 aagttccacg gtggaattgt aacacctgta ccaattgatt tatagaaata tttattgtgt
    30301 tcaccttcac ggtcaagtac cggcttacct tgcgctaagt ccatcattga acgcgcatag
    30361 tattcaataa aatcaatacc ttcagctgca tctccaaccg cttcatccca tggtttacct
    30421 gcttcatata ccataatcgc tgcaatttct gctttacgac gacgaataat tgctgaaaca
    30481 cgtaacataa gttctgcacg atcgttagca gaccatgttt tccaagattt ataagcttca
    30541 ttagctgctt taaatgcatc ttcaacatct tcttttgttg cttttgatgc atttgcaatc
    30601 acttgtgatg tgtctgcagg attgattgat ttaattttgt catctttgaa aatcttctct
    30661 ccattaatca ctaatggtat gtcttgacct aattcttttt ccacgtcttt caatgctttc
    30721 ttaaacatat ccacattttc ttggactgaa aaatcgtaac caggttcatt tttaaattct
    30781 actaccatgt acacttaccc cctataaatt ttgaaagtgg tttaaccctt tgatttaatg
    30841 atataacatc atttaaactc attttactat gattacggcc ggttttgcaa tcgctttcat
    30901 ttatataggc tatacacctt aacttatagg atttcacata tttttacttg atgctttaga
    30961 gctacaatga catatttatt tgttatcagt ctttaccaca aaattgtcca cccggatatt
    31021 cattatcaat ctcacgaata actttacaag gattaccaac cgctaaacta tttggtggta
    31081 tatctttggt aacaacacta cctgcaccaa ttacactacc ttctccaatc gtcacccctg
    31141 gtagcacggc tacatgaccc ccaaaccaag tattactgcc aatatgaatg ggtccggctt
    31201 tttcaaaacc ttcatttcta tgatgaaaat taagtggatg tgttgctgtg tagaatccac
    31261 aattaggtcc aataaaaaca ttatcgccaa ttgtaatctg tccaccatcc ataaaataac
    31321 aattggtatt tacaaagaca tttttcccaa gtttaacgtt ccatccataa tcagtatcaa
    31381 aaggaattgc gagttcgaca ttgtccgttg ttgtttgaaa taattgatca attaattcct
    31441 ttcttttatt tgtagcactc ggtcttgtat gatttaattc aaagcaaata tctttcgctc
    31501 gtgcacgttc attgattaag tcttgatcaa agtttgcatc gtaccatttt tctgctaaca
    31561 ttttttcttt ttcagtcatt aaacctttca actcctaata acttatttac ttgtttaaaa
    31621 gttaatcaaa taaacattcg cctatgcaac taatacgcta taacattatg aaatcatgac
    31681 cttatcaccc ttatctatac aattctcaca tcaaatactg ctaaagtagt agataaattc
    31741 aatactacag acgcattcat tttttaatct attaacgtac aatgtgagta agagaaacat
    31801 aaaggagtat gatagcgatg agaatattaa ttacaggcac agttgctatc ttaatcattc
    31861 taggtttagt taaaacgata caagattacg aaatgacaaa cgacacaagt cgtcagttgt
    31921 cagacaacaa agatgacgat aaagtcatcc atcttaataa tttcaaaaat ttacatgcga
    31981 aagaatttaa cccatctgat ttcttttaag tcacctaaga attgcaaatc cagaagtcat
    32041 ttaagtttta cctttcattc atacatcctt taatattaat tacgacttct tttatataga
    32101 tgctaaatag agagattgtt gtgcaatgtt tgcacggcaa tctctctttt tctttttaaa
    32161 attggtaaaa gtaaaacgca tcgattgact tatataccta tagggggtac attagacgtg
    32221 taacaatgaa tcacagggag gcaataatgt ggctaatacg aaaaaaacaa cgttagatat
    32281 cactggtatg acttgtgccg catgttcaaa tcgtatcgaa aagaaactga ataaacttga
    32341 tgacgttaat gcccaagtga atttaactac agagaaagca actgttgagt ataaccctga
    32401 tcaacatgat gtccaagaat ttattaatac gattcaacat ttaggttacg gtgtcgctgt
    32461 agaaactgtc gaattagaca ttacaggtat gacttgtgct gcatgctcaa gccgtattga
    32521 aaaagtgtta aataaaatgg acggcgttca aaatgcaacg gtcaatttaa caacagagca
    32581 agctaaagtt gactattatc ctgaagaaac agatgctgat aaacttgtca ctcgcattca
    32641 aaaattaggt tatgacgcgt ctattaaaga taacaataaa gatcaaacgt cacgcaaagc
    32701 tgaagcgcta caacataaat tgattaagct tatcatatca gcagtattat ctttaccact
    32761 attaatgtta atgtttgtgc atcttttcaa tatgcatata ccatcaatat tcatgaatcc
    32821 atggttccag tttatcttgg ctacaccagt tcaatttatt attggatggc aattttatgt
    32881 aggcgcatat aaaaacttaa gaaacggtgg cgccaatatg gatgtacttg ttgctgttgg
    32941 tacaagtgca gcatattttt atagtattta tgaaatggtt cgttggctaa atggctcaac
    33001 aacgcaaccg catttatact ttgaaacaag cgccgtacta attaccttaa tcttattcgg
    33061 taagtattta gaagctagag cgaagtctca aacaaccaat gcgctaggcg aattattaag
    33121 tttacaagct aaagaagcac gcattttaaa agacggtaat gaattgatga ttcctctaaa
    33181 tgaagtacat gttggagata cacttatcgt taaaccaggt gaaaagatac ctgttgatgg
    33241 caaaattatt aaaggtatga ctgccatcga cgaatctatg ttaacaggtg aatctatccc
    33301 tgttgagaag aatgttgatg atactgtaat tggttcaacg atgaacaaaa acggtactat
    33361 tactatgaca gcaacaaaag ttggcgggga cactgcgttg gcaaatatta ttaaagttgt
    33421 cgaagaagct caaagttcta aagcgccgat tcagcgattg gcagatatta tttctggtta
    33481 tttcgttcct atcgttgttg gtatcgcact attaacattt atcgtgtgga ttactttagt
    33541 tgcaccaggt acatttgaac ctgcacttgt tgcgagtatt tccgttcttg tcattgcttg
    33601 tccatgcgca ttgggacttg ctacaccaac ttctattatg gtgggtactg gtcgtgccgc
    33661 tgaaaatggt attttattta aaggtggcga atttgttgaa cgtgcgcacc aaatcgatac
    33721 tatcgtttta gataaaactg gcacgataac aaatggccgt ccagtcgtga ccgattatca
    33781 cggtgacgat caaactttac aactacttgc tactgctgaa aatgattctg aacatccgtt
    33841 agcagaagcc attgtcaatt atgcaaaaga aaagcaatta acattaactg agacaacaac
    33901 atttaaagca gtacctggcc atggtattga agcaacgatt gatcatcacc atatattggt
    33961 tggtaaccgt aaattaatgg ctgacaatga tattagcttg cctaagcata tttctgatga
    34021 tttaacacat tatgaacgag atggtaaaac tgctatgctc attgctgtta attattcatt
    34081 aactggtatc atcgcagtgg cagatactgt caaagatcat gccaaagatg ctataaaaca
    34141 attgcatgat atgggcattg aagttgccat gttaactggc gataataaaa acactgctca
    34201 agccattgca aaacaagtag gcatagatac tgttattgca gatattttac cagaagaaaa
    34261 agctgcacaa attgcgaaac tacagcaaca aggtaagaag gttgcgatgg ttggtgacgg
    34321 tgtaaatgat gcacctgcat tagttaaagc tgatatcggt atcgccattg gtacaggtac
    34381 agaagttgcc attgaagcag ctgatattac tattcttggt ggcgacttga tgcttattcc
    34441 taaagccatt tatgcaagta aagcaactat tcgtaatatt cgtcaaaatc tattttgggc
    34501 attcggctat aatattgccg gtatccctat agctgcattg ggcttacttg cgccatgggt
    34561 tgctggtgct gcaatggcac taagttctgt aagtgttgtg acaaatgcac ttagattgaa
    34621 aaaaatgcga ttagaaccac gccgtaaaga tgcctagatt ccttaataat gaaggatttg
    34681 ttggtgattc tgagatgggc tagtgattgg ctatattgag tgaacgtaat gttgaggttt
    34741 acagttggat cttcgctcca actgcatata tagttacact tttcgcttgg cgaattagtg
    34801 tatcttacct aatagctttg cctattaggt tccatcattt ttccaaataa taagtacact
    34861 atggtttaca gttggatctt cgctccaact gcataagagc cactaattat tataaataat
    34921 aagtggctct aaaaggaggt gaatgctatg tcacaagaaa ttttaaatgt tgaaggtatg
    34981 agctgtggtc actgcaaaag tgctgttgaa tctgcattaa acaatattga cggtgtcact
    35041 tcagctgaag ttaaccttga aaatggccaa gtaagtgttc aatatgacga cagtaaagtt
    35101 gctgtatctc aaatgaaaga cgcaattgaa gaccaaggtt acgatgtcgt ttaattaggc
    35161 aatattcaac gtcatcaaca ccaaatcaaa aaatcgaact gatgagaaac ccaacactcc
    35221 aaattatctc atcagttcga tttttaattt actcgtaacc tagtatctcc agtctgcaat
    35281 acatctaatg ttgcatctaa tgcatcgaca attagatttt caactgcagc ttcagtataa
    35341 aacgcaatac gtggcgttaa aataacatct tccctgccaa tcaacgactc tagcaatgga
    35401 tcattcaaag gtttgcccct ttggtcactc gggaaaagtt tgcgttcata ttcatacgta
    35461 tcaagtgcgg cacccttaat caagccgtta tccaatgcat caagtaacgc cttagtatct
    35521 actaaagaac ctctcgcaca attgacaaat actgcgccct ttttaaaatg tttaaataat
    35581 tcagcattaa atagataatg attatatttc gttgcaggta cacgtaatgt cacgatatca
    35641 gcaccttcaa ccgcttcctc aatcgtatct ttataatcga catacgttgc aattttagca
    35701 ttaggaaacg ggtcatatgc gaccacatca ctttgataac cattagcaaa tatatcggct
    35761 actacacggc caattcgacc tgtaccaata acagctactt ttaaatcttt aatggatttc
    35821 gataaaatag taggttccca tctaaaatca tgctctcgca ctttcgtttg aatttgatta
    35881 aaatgacgca caagattaat agcttggttc acagcaaact ccgcaattga attcggagag
    35941 tatgacggca catttgacac aatcaagtta tacttgtttg ctaactccaa atcatatgta
    36001 tcaaatccag cactacgttg tgcgatttgt ttaataccta gttcatttaa tcgtttataa
    36061 acatgctctg ataatggtat ttgttgtgat aacgataagc catcataacc agcgacacct
    36121 tcaacattgt catcagttaa tgcttcttta gtaatatcta cctcaacatg atgtttctct
    36181 gcccacgcct tgatataagg catatcctca tcacgtacac tcatgatttt aatttttgtc
    36241 attttaacat cacccttaac tttattattc atataaatat gctagttctg ttaatcttat
    36301 tgcagcttcg tctaatttct ggtcatctaa cgccaatgaa attctcacat aacgattacc
    36361 attctctcca aatggtttcc ctggagcaac aagtattgac ttctctttca ctaaaaattg
    36421 ctcaaattgc tcgctgtcat aacctgacgg tgtttccaac catacatata tgccaccttt
    36481 agcatgaaca aatggcaaat cagcttttgc aagcatggct tcgaatcggt cacgacgtgc
    36541 tttaaataca ttgctttgtt cttctaaaaa atcatcataa tgattcaaag catatattgc
    36601 ggcatcttgt aatgcaccaa acatcccagc atttgtgtgc gtttggtact ttttcaaagc
    36661 ttgaatcata tctttattac caactgcaaa accgactcta aaacctgaca tgttataacc
    36721 tttagacaag gaataaattt caatcgcaac atctttccca ttttccgagg caagtatact
    36781 aggattttta gcgtcgaaac cgaaagcacc ataagcaaaa tcatgcacga ttttagtgtc
    36841 tgtatcttta aatttagcta tcgcttcatc aaaaacttct ttcgtagctg tcgatccagt
    36901 tggattattt ggatacgtta aataaatgag ttttgtttta tctattattt gtgaatcaac
    36961 tttggaccaa tctggcaaat aatgcggcgg ttctaaatta agtggaacag gcttgccatc
    37021 agctaaaagt acacctgcta aataatccgt gtagcctgga tcaggtagta atacatagtc
    37081 tcctggattg ataacacatg ttggtactgc cactaatcca ttttttgtac catataaaat
    37141 gcatacttca tcttctttat ctaacgtcac attatattgt ctttgataaa aatctacaat
    37201 agcttgcttg aacgcttctt taccatgaaa agcaccatat ttttgatttt caggaatagt
    37261 tagtgcttgt tgaaaatgat caataatacc ttgtggcgtg ggcccatcag ggatcccaac
    37321 tgccatatta attaatggca atggtccatg ttcgatttta cgtcccatcg ttttcccgaa
    37381 ataactatca gggatatttg ctaatttgtt agagatcatc aaattcctcc tctatcatta
    37441 aacatagcct gggcgactat cataatccta acaacttgta tcactctcat ttagatggtt
    37501 acaatgacat cgccattcac cgttatgttc aacagaactt atgacacacg ttgtattgaa
    37561 tgaatttatt ttcattttag gtaggtacaa tattattgtc aatattagga attttcagat
    37621 taatatacac tcaatcgtta tgatttaact gccatgcata tccgcatgcg caaccagtta
    37681 gatatgctta tataaagtat aacgcccatc aaggtacgta ttcaaacgtg aaccttaaca
    37741 ggcgtcattc attgttaaat aaaacttctt aagcacatac ttatttcact atgcctttta
    37801 cgttcccctt atacttttct cacatctttc tattagacta ctcccttaca cgccctgctc
    37861 aatatcttta atcatttcat ctacagttat tttcgcactc gttaagacaa taggaacgcc
    37921 tgcacctgga tgcgtacttg cacctgcaaa atataaatct ttataatctc gcgatacatt
    37981 ttgtggacga taataattac tttgcgctaa agttggcatt aaaccgaatg ccgaaccaaa
    38041 tttcgcatga tacgtttgct caaaatcatt tggcgtaaag attgtttctg aaacaatatg
    38101 cgattttata tcttcaaata cttcaattgt tgctaattta cgataaataa tttcctttat
    38161 ttgttgcgtc aaagcttcat ctgaccaatc gattccgcta cctgttttaa gttccggagt
    38221 cggcattaac acataaatac cagttttccc ttctggcgca agtgatttat cagcgaccgc
    38281 tggaacatac acataaatag aaggatcata tgataaacgt ccctcaaata tttcttcaat
    38341 attgcctcta aaatcatctg aaaaaataac attatgaagt ctcacttgat ctgtcacatc
    38401 aatatctata ccgatataca ttaaaaatgc tgaacaagag taatctaagt ctgcaatttt
    38461 atgtggtgga tactttttaa taggtgcaaa atctggcatt aatgattccg caacattagg
    38521 gaaatcagcc gtacataaaa ttttatcaaa ttttcttatg tcaccattca cttttatcgc
    38581 atcagctcgt ttaaatttag gatcaatgat aattttttca atttctgcat caagttctat
    38641 atgaacacct aaatctttat tcaattgggc tagcccttga gccatgccgt acataccgcc
    38701 tttaataaaa tgcacaccaa acatcatttc aatcataggt ataatcgaat atagtgatgg
    38761 accacgtttg gggtcaattc caatgtacag tgtttgaaag gctaacagtt tttgtatctt
    38821 ttcgttatca atataatgct caattaattg atctgcatga tttagagttt ttaatttagc
    38881 cccttgtatt agagacgtca tattataaaa atcactaggt ttgcgatacg ttctttctaa
    38941 gaaatagcga cgtgcaattt catacttttt atacacgtca gttaaaaatg acataaaccc
    39001 atgtgtagaa ccaggctcta tactttctag catctgttga agttccgcta aatctgttgg
    39061 cactgtaata cgatcatcac gatcaaaata tacatcgtaa atatagcgta attgcttcaa
    39121 ttcaatataa tctttgtaat ttttaccaca cgctgtaaaa acatctttat atacatctgg
    39181 catcatgaca attgtaggcc ccatatcaaa tgtaaaccca tctttcttca actgattcat
    39241 ccgtccgcct acatggctat ttttttcgaa gatagtcact tcatgccctt gagaagcaat
    39301 acgtgctgcc gctgctaatc ctgtgacacc tgcaccgata actgcaattt tcattattca
    39361 accacctata ctttatgata tttactattt atttcatgaa ataaatctgc ctttttcttt
    39421 ttatcgacaa aaacacgctc atgtaatgta tagtttgctt gtcgtacttc atctaatatt
    39481 tcaatatata tacgcgctgc taattctatg attggttgtg cctctatgct aaatacttta
    39541 atttgatcca tcacatcttg gaagtctttt tctgctatag ctgcataata ttcccataag
    39601 gcaatataac gttcatcaat acccttatga tatacttctg caatatcaac ttcatattga
    39661 ttcaatcgct gtttactaaa atagatacgt tcattttcta aatcttcacc aacatctctt
    39721 aatatattaa ttagctgcag cgactcccca agtcttctag cgacgtcata tgtctgttgt
    39781 gttgtataat cacttaaaat aggtgttaat acttcaccaa ctgtaccggc aacaccataa
    39841 caatatccta ataattcagt gtctgtttca aacattgtaa aatgttgatc cttatatacc
    39901 gtatcgatta aattgtaaaa agattgaaaa gcaatttgat tgtgctgtgc aacatactga
    39961 agtgctaaca tgatacgacg atcactttga aagtgatgat gctccaaggg gtgtttttcg
    40021 ataatttgta tgtcttcttt tatttgattt aaaaattgaa tatcaccata aacatcaata
    40081 ctgtcatcaa tctttcgaca tacagcataa attgcccaaa ccgctttcct ttgattcttt
    40141 ggtaataaat caaatgcata tgaaaagctt tttgaatgct tcttcatgat tttatgacaa
    40201 tacttaaaat tcatatccat cattgtcata ttaatcctcc taaatagtta tgccatcatt
    40261 taaacttatt tttcttttgt taattgatat tgacgacctt tccattcaac tacaccataa
    40321 cgatgcgttt gtttccaaga ttggatgaaa attttagtga aaaacataaa taacaatgga
    40381 tgacatacca ttaataaatt agaaaattgg ccgacccttc gatgcagata aataaattgt
    40441 gtagtatagc tcaagtaaac tgttaacatt tttcctacag acatttggcg atattttaaa
    40501 ctaagacata gccctaaaat actcgcaata gaaccgaaca accacaacac aattgctgtc
    40561 atgatcttag gatttgtgcc acctgcccca gttgacaaat gctttgtcca tccttcttgt
    40621 aatgactgaa atccttcttg atacatacgg aatgcgacaa atggaaatcc ttcataaact
    40681 gttacgggca atgattgaga agcatatgca tttcctaaag caaatccttc aataatatgc
    40741 tggttggcat ttttatgacc gtcagttgtg taataatccg ttttatttgt taatgtcaca
    40801 ggaccaaacg cttgctggtt agtctgaccg tcgtctaaag tagaaaatac attcatacca
    40861 acgacagtca ttaaattaaa tatcgctgaa aatccttcat agcagcgctt tgttatatga
    40921 taaggctgta cacttaacaa tcctttttca ccttgtaatt gatactgatt aatcaacgtt
    40981 tcaacagcat ctttccttaa gaaagttaca tcagcatcta caaaggcaat actattcgta
    41041 catgcatgcg tcacaccttg ataacaagca tgtgatttcc cataccattt gccgtcagta
    41101 tcatcaacaa catctactac agtcgcacca tatgaacgtg ctacacgtgc tgtttcatct
    41161 gtcgatccgt cattcataac aatgacatct actggaactt gctgttgtat tatcgaatgt
    41221 agtaaatgac ctattctttt ttcttcgttt ctagccggaa taataattgt taatgcttta
    41281 tgattgaagt tcagcgtttt cgcctttagc tgatgtcgac gattaaacat caatgcacca
    41341 cacgccatag acatggtcac tattactgtt aatattcgtg ataaccattt catagatatc
    41401 acttcctatt cttcacttct cgcgcgttta ttttgtctgc tacttgctgt ccacttaagg
    41461 taaccattgg cataccaccg ccgggattta ctgaaccacc tacaaagtat aagttttcaa
    41521 aatactggct ttctttagga aatttaaatc ctttgttttt ctttttatct gctacaacac
    41581 catatattgc accacgatta gaatgataat ttttttctat atcttctggc gtccagacat
    41641 cttcataaat aatatgcttt cttaagtcag tgagtcccat tttttctaat ttatccaaaa
    41701 ttttattcct aaacttcgca taatcttcag tggtaaaagg ctgatcttga atatatggaa
    41761 tatgtggtaa gactttgata ttttcataac ctactggcgc ttgtgtgtga tcagttttat
    41821 tcgtatttac tagataaatg gtcggatcat ctggcaatac cttttcatga aaaacttgtt
    41881 gataattgag ataagcattt tccgtaaaaa agaaattatg atgtgctaat tgcgggtatt
    41941 ggcaagcaac acctaaatgc atcacatatc ctgagcttgc tggctcaaat tcccgctcta
    42001 atttgtttaa tcgttgagta tcaaggtgaa gtaaatattt ataagtaggt atgacttcca
    42061 tatttgaaat aatataatct gcctttacaa actcacctgt atctaatctg acacccgtca
    42121 cacatctttg atatgtttta atattgtcca cacgtgcacc tgtatgaatt gtgacacctt
    42181 cttcacgggc tagcttttcc aaggcattgg ctaaatgatg gattccacct tctacatacc
    42241 aaaggccttg ctcttgttgc atatggaata acatagataa tacagctggc gcatcgtatg
    42301 atgaagaacc tacatatttg ataaaatagc ctaacatttg tcgcaagtat ggattcgaga
    42361 tacgcttgtc tatggcctgt tgcatagtat gtacataatc ataattaata agtgcattta
    42421 atggcccatg aaatttaata atttgagata gtgtatctaa accatagtta aaataccctt
    42481 tttcagtaat acgatcgatt cgtcttgtat aattcaaata attttgcagt tcctctatat
    42541 ccattttcga caatatcgca ttatgctgac ctgtttcttt aataccttca tacaaatcga
    42601 tagtcgttcc atctggaaaa aagctacgcc attgatgtgg caatctcttg attgtaacgt
    42661 agtctgacat ttgcttattg ctatattcga ataatttttc aaaaatataa ggcatcgtta
    42721 aaatagatgg acctaaatca aagccaaagc catctgattc atgacgattc actttgcctc
    42781 ctatatgatt attttgttca tataatgaga ccgaatagcc actttgcgcc attcgaattg
    42841 ctgcagaaat cccacctaag ccaccaccaa taacgatgat atgtttagtc atgacgttca
    42901 cctcttcgtt ttaatatgcg tagtaactgc gttaatctcg gtcgattata acgttgcaca
    42961 ataatgatag gaacattagc tatcattgca tagaaaatat ttatatactt tactaaacga
    43021 gggcctttat tgaatatgat gactggaagt atcgatatcc aatgaattaa ctctgccctc
    43081 tttgtctcta taatcatttt ttcaatttca tcagaattga ttgtcattaa atgacgctga
    43141 tcatatatat ttcgattaag ctgatgacca tctaaaatct tatgcttcca ttttcttacg
    43201 taaaaccatt tgttccaaaa ttttccatgc ttctcaaagt taaatgattt aaatattata
    43261 tgcttctgac gaaaatactt gttaggaatt cttgtaccta aatttgctat atttagttga
    43321 acaattaacc aatacatact acagaaaaat gctgttttga tatatttttt catggttttc
    43381 atctaaattg aatcactctc aatcatactg acatagtata aattatcatt ttattactta
    43441 gttccctaaa gatcagtgaa taaaccgctc tcatttatat ttatataaaa caaaaacgcc
    43501 aatactctca tgttagagaa tattgacgtc atttgattta catttgatta atgaatgaaa
    43561 ttatatgaac ctgcttggtt tgctgaaatt gtacgagatg taaccacacc agcaccatgt
    43621 ccatagttca tttctgaaac acgaacagaa ccgttgctgt taacgccttc aacgtaagca
    43681 acatgaccgt aatagccttg tgttgtttgc atgatagcac cagcttttgg tgtattgttc
    43741 actgtatagc cagatgatgc agctgcgcta gcccaattac tagcattgcc ccatgttgaa
    43801 ccaattttcc caccaacacg atcaaataca taataagtac attgtcctga agtatataag
    43861 ttacttcctg atgcataacc attagaaatt gaacgaccat ttgaagatgg cgctgcagtt
    43921 gtagtcacat gaacattatt gcttgttgtg ctataacttg cacctgaacc accagtgtaa
    43981 tagttatttg ttgctgtttg gctattgttt gtatagttat tgtattgata tgaatgatta
    44041 tagttattgt agttattata gctataatta ttatatgaat tattgtaagt attatagtta
    44101 ttgtagtaat acgtattgtt ttgttctaat tgacttggat tccaatttcc agtccaagtg
    44161 taatgataat taccttgtgc atcaattgta tacgtatagc tgtatgattg agcgtcatta
    44221 gaattatatc cattgttatt ttgttctgcg gcttgtgcat catgtcctgc aaatgcgata
    44281 gtagcaagtc ctgctgtagc gattgtagct gtaacgattt ttttcataat taaatatcct
    44341 cccaataatc aaaacactct atacatatag tcattagcga ctcaattatt ttaaatttta
    44401 tattttttat ttttttagta tgcaaataat tataactttt atagttttgc gacaaaacaa
    44461 actataacag agattaggca ccttgtggac tttgttatca taatgtaatc tgttcctaac
    44521 aaagtatcat tgaatgtaat ataccaatta gaatgtttgc aacacttttt ctgcaccgtt
    44581 aacataggat ttgaaaataa aaaagcacta aacttttaat gaaatacctc ctttttgata
    44641 ataaatatca gaagtaatgt aatatttcaa aagtttagtg cttcaagtta tttcttattt
    44701 gtagcatgtg tttccatcgt ttttacaatc aaatcagtca gcgctttact acctgcatat
    44761 tctaagtgaa taccgtcata tgcaaagtac tccggatgac cttcagaagc tttataccaa
    44821 tcgactagat gtacattaga gcgcttttca gctgcctcat aaattaattt atttattcta
    44881 ccttcataat ctctaggtac tctaatagaa actaaataaa tgtctgcttt tccaaaacta
    44941 tccaatagtt catttaattg atttttcgta aatgccccat ttgtaccaag ctctactaca
    45001 actttttgac cttttttagc ataatcttta tattgcgatt tcacaattgg tgtagcatca
    45061 acgagttgcc gtccaacttt accatcaatt tgtgcatttg gtattttctt agtaaagaca
    45121 ttaccaatat ccaccatgac cgagtcacca attaataacg gtgatgactt tttaatatcc
    45181 tctttactat tcgctgcctg tttatcctct tgcttatctt ttttaacaac ttttttcttc
    45241 gttgttttaa attccgtctt cttctctttc ttctcggctt catgttgttt gccaagtgca
    45301 tcaaactgtc cactgagcac aacgatagac ggaaccaata ataggataac taacactgtt
    45361 ctagcaaatt gccccttctt tttaggtaaa aatgcaaaag ctttaaatcc ttttttacgt
    45421 ataggtgttt caataaagcg atacgaaatt tcagccatta acgctgttaa caaaatttct
    45481 ataatataaa cgtataccgg tatttgtcct tgtacgtaat aactgttcac aaaaacaatg
    45541 ataggataat gccataaata taagctatat gatcgtttac ctataattag caaaggtttc
    45601 atacttaaaa atttagcaaa taaactagaa ggatgtaccg caattgcaat aatgaataaa
    45661 gttgcaaatg atataatgta aaatcctcca ttatatatcc attgatcttg gtctccaact
    45721 ataaagaaca aagtcattag caccgcaaaa ccagatatcc ctataatatc taatgatacg
    45781 acaatctttt tagaaatatc ttttttcaaa gcaaacggtg gccaaataaa tgctaatata
    45841 caaccaagca ataaagtttg cagtcgtgta tctgtcccaa aatacacacg tgaattatct
    45901 ccagtgataa aatgaatcac tatcataagt cctaaagaaa tcaacgatac aataaatagc
    45961 gtttgaataa tatttctcgg tttaaatcta tgtaataaga acgtgataac caatgggaaa
    46021 agcaagtaaa attgttcttc aatggctaaa gaccataaat gtttgagtgg ttcaatagca
    46081 aattggttaa aataatctac attctgtgaa atgtaccacc agtttgaaac atagaatata
    46141 gctgcaatag catctcgttt catttgtata atcaattccg gtttaaatat cagtgtgaac
    46201 gtaagcacga cacagattaa aaataacact gccggaatga gtcgtttcaa tcgtcgcttc
    46261 caaaactcta gcaaatcgat tttttgcgtc cgataatact cacttatcaa caaacttgtt
    46321 attaaataac ctgaaataac gaagaatgta tctactccta aaaagccccc acttaaccat
    46381 tgtgcattca agtgataaat aatgattcct ataactgcga atgccctcaa tccatctaat
    46441 ccgggtaagt atcgcgggga atacattttt tctaaacgtt taaagtcttt tgtatccatg
    46501 ttaataaacg ccccatttat tttt
//
LOCUS       DAHHRR010000009        40496 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.009, whole genome shotgun sequence.
ACCESSION   DAHHRR010000009 DAHHRR010000000
VERSION     DAHHRR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 40496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.009"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(85..801)
                     /locus_tag="NBI42_000390"
     CDS             complement(85..801)
                     /locus_tag="NBI42_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1776854.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDESYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNRDEHNEDAERLFFIAKK"
     gene            complement(804..1157)
                     /gene="rsfS"
                     /locus_tag="NBI42_000391"
     CDS             complement(804..1157)
                     /gene="rsfS"
                     /locus_tag="NBI42_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCD1776855.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYGQVAY"
     gene            complement(1158..1742)
                     /gene="yqeK"
                     /locus_tag="NBI42_000392"
     CDS             complement(1158..1742)
                     /gene="yqeK"
                     /locus_tag="NBI42_000392"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCD1776856.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            complement(1732..2301)
                     /gene="nadD"
                     /locus_tag="NBI42_000393"
     CDS             complement(1732..2301)
                     /gene="nadD"
                     /locus_tag="NBI42_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCD1776857.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMITFVVVNRDKNSQNVDNAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            complement(2304..2594)
                     /gene="yhbY"
                     /locus_tag="NBI42_000394"
     CDS             complement(2304..2594)
                     /gene="yhbY"
                     /locus_tag="NBI42_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCD1776858.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATRSELVQVIGSMIVIYRESKENKEIELP"
     gene            complement(2598..3404)
                     /gene="aroE"
                     /locus_tag="NBI42_000395"
     CDS             complement(2598..3404)
                     /gene="aroE"
                     /locus_tag="NBI42_000395"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCD1776859.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELDGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIIYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            complement(3418..4518)
                     /gene="yqeH"
                     /locus_tag="NBI42_000396"
     CDS             complement(3418..4518)
                     /gene="yqeH"
                     /locus_tag="NBI42_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCD1776860.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEAEDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTENANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            complement(4519..5046)
                     /locus_tag="NBI42_000397"
     CDS             complement(4519..5046)
                     /locus_tag="NBI42_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCD1776861.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            complement(5066..5752)
                     /gene="mtnN"
                     /locus_tag="NBI42_000398"
     CDS             complement(5066..5752)
                     /gene="mtnN"
                     /locus_tag="NBI42_000398"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCD1776862.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDIIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            6065..6334
                     /locus_tag="NBI42_000399"
     CDS             6065..6334
                     /locus_tag="NBI42_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776863.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIKFDAFIIIVFIFFLL"
     gene            complement(6536..7287)
                     /locus_tag="NBI42_000400"
                     /pseudo
     CDS             complement(6536..7287)
                     /locus_tag="NBI42_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
     gene            complement(7735..8964)
                     /locus_tag="NBI42_000401"
     CDS             complement(7735..8964)
                     /locus_tag="NBI42_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCD1776864.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            complement(8976..9728)
                     /gene="pxpA"
                     /locus_tag="NBI42_000402"
     CDS             complement(8976..9728)
                     /gene="pxpA"
                     /locus_tag="NBI42_000402"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCD1776865.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNEEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDRGIASVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            complement(9728..11089)
                     /locus_tag="NBI42_000403"
     CDS             complement(9728..11089)
                     /locus_tag="NBI42_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCD1776866.1"
                     /translation="MLCCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIEKLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVVAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRGSAIDKLKATLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            complement(11103..11552)
                     /locus_tag="NBI42_000404"
     CDS             complement(11103..11552)
                     /locus_tag="NBI42_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCD1776867.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMVGTFFLQDSKELTEPIVKVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            complement(11554..12564)
                     /locus_tag="NBI42_000405"
     CDS             complement(11554..12564)
                     /locus_tag="NBI42_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCD1776868.1"
                     /translation="MSIKILQPGLFSTVQDLGRKGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSVNASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHTINVPPIAECYATHTRSSIGGFKGRKLLANDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHEKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            complement(12554..13288)
                     /gene="pxpB"
                     /locus_tag="NBI42_000406"
     CDS             complement(12554..13288)
                     /gene="pxpB"
                     /locus_tag="NBI42_000406"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCD1776869.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITPSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLYTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     EFIQIERDISDGNFNVDDWVVIENVN"
     gene            complement(13614..14090)
                     /gene="greA"
                     /locus_tag="NBI42_000407"
     CDS             complement(13614..14090)
                     /gene="greA"
                     /locus_tag="NBI42_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCD1776870.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            complement(14118..14741)
                     /gene="udk"
                     /locus_tag="NBI42_000408"
     CDS             complement(14118..14741)
                     /gene="udk"
                     /locus_tag="NBI42_000408"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCD1776871.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            complement(14741..16009)
                     /locus_tag="NBI42_000409"
     CDS             complement(14741..16009)
                     /locus_tag="NBI42_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD1776872.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDSGELDVFYDQGEVKPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPENFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            complement(16021..16944)
                     /locus_tag="NBI42_000410"
     CDS             complement(16021..16944)
                     /locus_tag="NBI42_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD1776873.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            complement(16947..17585)
                     /locus_tag="NBI42_000411"
     CDS             complement(16947..17585)
                     /locus_tag="NBI42_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCD1776874.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALDQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            complement(17870..18178)
                     /locus_tag="NBI42_000412"
     CDS             complement(17870..18178)
                     /locus_tag="NBI42_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCD1776875.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            complement(18193..18621)
                     /gene="ruvX"
                     /locus_tag="NBI42_000413"
     CDS             complement(18193..18621)
                     /gene="ruvX"
                     /locus_tag="NBI42_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCD1776876.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            complement(18625..18885)
                     /locus_tag="NBI42_000414"
     CDS             complement(18625..18885)
                     /locus_tag="NBI42_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCD1776877.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            complement(18948..21578)
                     /gene="alaS"
                     /locus_tag="NBI42_000415"
     CDS             complement(18948..21578)
                     /gene="alaS"
                     /locus_tag="NBI42_000415"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCD1776878.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPSEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKVTTNEISGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITFASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAVSGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            complement(21925..24402)
                     /locus_tag="NBI42_000416"
     CDS             complement(21925..24402)
                     /locus_tag="NBI42_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCD1776879.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEASLSSNDSEEVTSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            complement(24404..25072)
                     /locus_tag="NBI42_000417"
     CDS             complement(24404..25072)
                     /locus_tag="NBI42_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD1776880.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEEDPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGIVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            complement(25644..26762)
                     /gene="mnmA"
                     /locus_tag="NBI42_000418"
     CDS             complement(25644..26762)
                     /gene="mnmA"
                     /locus_tag="NBI42_000418"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCD1776881.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            complement(26763..27905)
                     /locus_tag="NBI42_000419"
     CDS             complement(26763..27905)
                     /locus_tag="NBI42_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD1776882.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            28217..29230
                     /locus_tag="NBI42_000420"
     CDS             28217..29230
                     /locus_tag="NBI42_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCD1776883.1"
                     /translation="MAIKYSALNLVPIREGEDERTAINDMVKLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINKK
                     NTL"
     gene            complement(29467..29613)
                     /locus_tag="NBI42_000421"
     CDS             complement(29467..29613)
                     /locus_tag="NBI42_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776884.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
     gene            complement(29653..29835)
                     /locus_tag="NBI42_000422"
     CDS             complement(29653..29835)
                     /locus_tag="NBI42_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCD1776885.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            complement(29935..30357)
                     /locus_tag="NBI42_000423"
     CDS             complement(29935..30357)
                     /locus_tag="NBI42_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCD1776886.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            30442..31716
                     /locus_tag="NBI42_000424"
     CDS             30442..31716
                     /locus_tag="NBI42_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCD1776887.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDEEKGLKTYQPKIDEDAMAYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            complement(31877..32650)
                     /locus_tag="NBI42_000425"
     CDS             complement(31877..32650)
                     /locus_tag="NBI42_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCD1776888.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(32650..32777)
                     /locus_tag="NBI42_000426"
                     /pseudo
     CDS             complement(32650..32777)
                     /locus_tag="NBI42_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32842..33032)
                     /gene="ssrS"
                     /locus_tag="NBI42_000427"
     ncRNA           complement(32842..33032)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="NBI42_000427"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(33110..34876)
                     /gene="aspS"
                     /locus_tag="NBI42_000428"
     CDS             complement(33110..34876)
                     /gene="aspS"
                     /locus_tag="NBI42_000428"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCD1776889.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            complement(34892..36154)
                     /locus_tag="NBI42_000429"
     CDS             complement(34892..36154)
                     /locus_tag="NBI42_000429"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCD1776890.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIAYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            complement(36615..37490)
                     /locus_tag="NBI42_000430"
     CDS             complement(36615..37490)
                     /locus_tag="NBI42_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD1776891.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            complement(37487..37939)
                     /locus_tag="NBI42_000431"
     CDS             complement(37487..37939)
                     /locus_tag="NBI42_000431"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCD1776892.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQDSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVNINNDGPVTIIYESQDGKIQ"
     gene            complement(37951..40140)
                     /locus_tag="NBI42_000432"
     CDS             complement(37951..40140)
                     /locus_tag="NBI42_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCD1776893.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
BASE COUNT        12481 a         7868 c         5508 g        14639 t
ORIGIN      
        1 taaaaaatct ttatagcgta ataaaaattg atacaataaa accactcctc aactttatac
       61 acgtctgagc gagtggtttt acttttattt tttcgcaatg aaaaacaatc tttctgcatc
      121 ttcgttatgt tcatctctat taaaatcagt aaatgtttca acatgtttaa aaccaacttg
      181 agctaaccaa gacaaatatg tcttttcatc aaatgttctc tgaaaatgag actcatcaaa
      241 tcttgaatat gattcatctt catgtcgaat gaaaaatgtc atatcatgat aaacacttaa
      301 aggtaaatct ccttgcacag catcccatgc taaaaaaatg tcccctttat catcaatata
      361 actttgatta ttaaacaaag tcatcatttt ataaacagta tgtacatcaa aaataaatac
      421 acctgaatca gtcagatgat gataaacatt gatgaatgtt tcaataactg cagtttcatc
      481 ttgcaaataa tttagagagt cacaaaaaat agtgatgata tcaaattgtt gttgcaaatc
      541 aaaagatgtc atatctcctt caagccaatt tacatttgct gatttttgag ctgcaacagt
      601 caacatatca acacttaaat ccataccagt aacattacct aaagcttcta attgaactgt
      661 taaactacca gtaccgcatc caatatctaa aatatttgat tcatctttgc agtgattttt
      721 tacaatttca aaccattttt catatggttg atcttgagtc aattgatcgt acactaggct
      781 catttctgca tattgcgaca taattaatac gcaacctgac catatgattc taatggtgca
      841 tcttgatata acttttcaat attataataa tttctttcgt ctttatggaa aacatgtacc
      901 acaacatcag ctaagtcaat taatatccaa cgcgcttcat tgtatccttc catacgtttt
      961 acttctatat tttgttcatt ggctacttct ttcaccgctc tagcaatcgc ttgaacttgt
     1021 cgttcattat ttccgtgcgt tacaacaaaa taatctgtca tatcgctgat acctttcatt
     1081 tctaaagaaa tcgtatcttc gcctttttta ttgtcaattg catccacagc aattgctaat
     1141 aattcttgtg aattcattta atcatccttt attctttcgt cactatagtt ataataattt
     1201 aaacagtcaa tcgtcttatt atataccgta atatctttct gtattaaaaa tagtactgtg
     1261 cgtttagaaa tttcataaat tgtcttatct aaactacctt gattgtatgc catatctcga
     1321 atatcatcaa ctcctgggat tgttcttcca ggttcgatgt aatctgcaat aaaaatcagt
     1381 ttttcagttt ttgtcatttg ttgacgtcca gtagtatggt atttgatagc cattaatact
     1441 tcctcatcat tgataccata ttcatgttcc atgattgctg cacacacagg accatgcaat
     1501 atttcactac cataactcag tagatcatta cctaattcat attgtcgaac aatttgatac
     1561 attttaccta aatcatcata tttacagaaa tcatgtaata cacctgctaa ttctacttta
     1621 ctagtgtctc catcataaat ttctgctaat ttaatcgctg tttctgcaac tcttaaagaa
     1681 tgattataac gtttctctgg cagtttctct tttgcaagcc gttttgcttt ttcaatgttc
     1741 atataatcct tcccccttaa tatagttttc aacggattta ggaacaagaa cttggataga
     1801 tttcccttca ctaactcttt gtcgaatcat tgtcgaactt atatctaccc taggtatctg
     1861 aattgcaatc atagcattat caacattttg actatttttg tctcgattta caactacaaa
     1921 agtaatcatt tcttttaagt attcaatttg ataccatttc tctagttggt tatactgatc
     1981 tgtcccaata acaaagtaca actcactgtc tttgtgttgc tccttgaatg ccttgattgt
     2041 gtcataggta taactttgac caccacgttt aatttcatcg tcgcaaatat ctccaaaacc
     2101 aagctcgtcg ataatcatct gtatcattgt taatctgtgc tgaacgtcta taaaatcatg
     2161 gtgctttttc aatggagaca taaaactagg taaaaaataa aattcatccg gctgtaattc
     2221 atgaaatact tcgctagcta ctatcatatg tgcagtatga atagggttaa actgaccgcc
     2281 gtaaagtact atctttttca ttattatggc aattcaattt ctttattttc tttagattct
     2341 ctataaatca ctatcataga tccaatcact tgcactaatt cactacgcgt agcttcgctt
     2401 aatgtttcag ctaattcttt tttatcatca aagttatttt gtagtacatg tactttaatc
     2461 aattctctgt tttctaacgt atcatctatt tgtttaatca tgttttcgtt gataccgcct
     2521 tttccaattt gaaaaatcgg atcaatattg tgcgctaaac ttcttaagta tcttttttgt
     2581 ttgccagtaa gcatatgtta ttctcctttt aattgttgta aaactgctgt tttcatagaa
     2641 ttaatatcag catctttatt agtccaaatt ttaaagcttt ccgcaccctg gtaaacaaac
     2701 atatctaagc cattataaat atggtttccc ttgcgctctg cttcctctaa aataggtgtt
     2761 ttatacggta tataaataat gtcactcatt aaagtattgg gagaaagatg ctttaaatta
     2821 ataatacttt cgttatttcc agccataccc gctggtgttg tattaataac aatatcgaat
     2881 tcagctaaat acttttcagc atctgccaat gaaatttggt ttatatttaa attccaagat
     2941 tcaaaacgag ccatcgttct attcgcaaca gttaatttgg gctttacaaa ttttgctaat
     3001 tcataagcaa tacctttact tgcaccacct gcgcccaaaa ttaaaatgta tgcattttct
     3061 aaatctggat aaacgctgtg caatcctttc acataaccga taccatctgt attataccct
     3121 atccacttgc catcttttat caaaacagtg ttaactgcac ctgcattaat cgcttgttca
     3181 tcaacataat ctaaatacgg tatgatacgt tctttatgag gaattgtgat attaaagcca
     3241 tctaattctt tttttgaaat aatttcttta attaaatgaa aatcttcaat tggaatattt
     3301 aaagcttcat aagtatcatc taatcctaaa gaattaaaat ttgctctatg cataacgggc
     3361 gacaaggaat gtgaaatagg atttccgata actgcaaatt tcatttttta aatcacctta
     3421 taaaatagaa tttcttaata caacatcaac atttttagga acacgaacga ttactttagc
     3481 tcctggtcct atagttataa agcctagacc agagatcata acatcacgtt tctctttgcc
     3541 tgtttcaagt ctaacagcct ttacctcatt aagatcaaaa ttttgtggat ttccaggtgg
     3601 cgttaataaa tcgccaagtt gattacgcca taaatcattg gcgttctccg ttttagtacg
     3661 atgtatattc aagtcattag aaaagaaaca aactaacgga cgtttaccac ctgatacata
     3721 atctatgcgc gctagaccgc cgaagaataa tgtttgcgcc tcgtttaatt gatatacgcg
     3781 ttgttttatt tctttcttag gcataataat tttcaattct ttttcactaa ctaaatgcgt
     3841 catttgatga tcttgaataa tacctggtgt atcatacata aatgatgttt catctaaagg
     3901 aatatctatc atatctaaag ttgttccagg gaatcttgaa gttgttacta catctttttc
     3961 accaacacta gcttcaatca gtttattaat caatgtagat ttcccaacat tcgttgtccc
     4021 tacaatatac acatcttcat tttctcgaat attcgcaatt gatgataata agtcgtctat
     4081 gccccagcct ttttcagctg aaattaatac gacatcctca gcttccaaac catattttct
     4141 tgctgttcgt tttaaccatt ctttaactcg acgtttatta atttgtttcg gcaataaatc
     4201 caatttgttt gctgctaaaa tgattttttt gtttccgaca atacgtttaa ctgcattaat
     4261 aaatgatcct tcaaagtcaa atacatccac aacattgacg acaatgccct ttttatccgc
     4321 aagtcctgat aataatttta aaaagtcttc actttctaat cctacatctt gaacttcgtt
     4381 ataatttttc aagcggaaac aacgtctgca aatcacgtca tcacgaaaca tattatgctc
     4441 tggtacaaaa ccaggtttat ttttatcttc agattgaagt ggcgcaccac aaccgataca
     4501 ttttaaaatg tcagacaatc aattttcctc ccatgtgata taaccttttt tactgaaatg
     4561 acgtaataat cgtctttcaa ttaatctatt aaacttagta ataaagccat cagttcgttt
     4621 aactggaacg accataattg tatataaacc tcgacgatta ccaccaaata catcagtaag
     4681 catttggtca cctattacaa cagtttgatc tggtctaata ttcatcttag ttattgcttt
     4741 atcaaacgct ttccccattg gctttctcgc tttaaaaata aaatcgatgt ctaaatgctg
     4801 actaaaacta gcaacacgag actcattatt attagacacg attgtaatag tgattccttt
     4861 ttcattagct tccttaaacc atgctttaac acgttctgta ggttctttaa catcccaacc
     4921 tactagcgta ttatctaaat ctgtaataat acctttaacg cctttgtcca ctaacttgtc
     4981 taaatcaatt tgaaatattg attgaacata tgaattcggc ataaaaaact tgcgaactaa
     5041 acccatttaa ctcaccttta cctttttata attgagacac taatgcttca acagtttgac
     5101 ttgatgatac agctgctttt tctaaaaatg cttcgaagct catttccgct tctccatttg
     5161 ctaagtctga aactgcacga actacaacaa atggtacatt aaattgataa catgtttgtg
     5221 caattgcagt tgcttccatt tcaaccgcca tcgcatttgg aaatgctttt ttaatttttt
     5281 ggcgttgttc aacactaccg ataaagctat caccacttac aattaagcct actttagctg
     5341 ttaattgttg ttgttgtaca acttgagata ctttttctat taaaggttta cttgattgaa
     5401 atgctaccgg catctgtggt atttgtccat attcataacc aaatgctgtt gcatctgcat
     5461 catgatattt tacatcatca cttataagaa cgtcacctac atttaaactt tcatctaaag
     5521 ctccagcaga acctgtatta ataatgatgt ccggtttaaa cttattaatt aataatgtcg
     5581 tagaaattgc agcattaact tttccaatgc cgctttgggt aatcactact tctctatctt
     5641 ttaaaatgcc agtataaaat ttaacatgtg caactgaaat ttcgcttaat tgtgttaatt
     5701 tattttttaa tattgttact tcttcttcca tggcaccaat tataccaatc atcctttgat
     5761 tcacctcttt taaaaaatcc tagcatttgt tattttatca cattttaatc actacaacga
     5821 caagtatcat gattttcatt actatataaa acttttgctt tattttaata aaaacattca
     5881 caataacgtg gtatcataaa taattattta tgaactcata acagtgaaat cgatgcttct
     5941 aatttctgca tttttcaaaa actaatcata tcaattacat caaccaatcg tgtcaagaaa
     6001 ctttattaaa taacaggaaa atgatatgtt taaaataata atgatgaaat aagaggattt
     6061 tacaatggct gaaaataatc aaaatagtct cgtaacaaag atagctacat acggcagttt
     6121 tattgcaatt gcgtcgtttg tcattttatt tataagtata tttttaaagt tttcatttaa
     6181 tatcgaattt acagcaacaa ttatgaacat atgtcgctac actctaattt taggatttat
     6241 acttatgtcc ttacctgatg ttgttgataa aaacatcaag aaaattaaat tcgatgcatt
     6301 tattatcatc gtctttatct tctttttact atagtagttt tattttgtaa ttacacagtt
     6361 aatcatattt gtttcgtatc gaattcctgt gtgcattaaa gatcattata atagaaaaca
     6421 gcagtaagac ttttctaagt gttagtatcc ttactgctgt ttttatttgt aacaatataa
     6481 tgacatgtac tttatatttc actgtcaaag attactaatg attaacttac ttaaattatg
     6541 atttgaataa atagatatct aaatgcaatt tgtcactatt gatagtttta ttatcagcat
     6601 acatttttaa tacgtcatga ccatactcac catttaaatt ataaaaatca tattctacat
     6661 tttttcatca ttattatgat atataacata cccttaaaca atgtcgccac cttattcata
     6721 caacttatac tttttattca aataatttct taattgaaca tctaattctt gaatggtaac
     6781 aattttcttt ttcgttttta ctgcagttag ttctatttct gtttgatgtc cgtcaatcca
     6841 taaattacaa ggtatattct tatcattatc gtacttatta ttgtcaatta aagtaaccac
     6901 tccataacta cattgcgttt tattggtttc tcccccatga cattcatagc tatagcttcc
     6961 agcatatata tcgatattct tatttataaa ttcattggaa agtgactcat tttcgaattc
     7021 aactttaaaa tcctttttcg aagcatcatt taagaacatg tcatgaaata acaaatcatg
     7081 agtcaacagt ctatcgtttg tttttttatt ttcaagctga gttggattga tgaatgacat
     7141 cttagcatta cttagacgct ttgaattaag tttactttta tgatgtaagt ctgaatattc
     7201 gattgcactt gctgaatttg tcaaaattcc ttctaacaat aatattaaaa taaatcctga
     7261 taaatattta acttcaaact cccccaaaat caattattaa tttacatttg agagcaagac
     7321 ataaatgata aagaaccact tatggtttat tatgtagtgg ttcttgagcc tacaattttg
     7381 atttatgtat cacactcata atacttttca atttgaaata ccctgttcgc aattattagt
     7441 tgagttatat caaccaatat agtgtaaaca taaaaattcc aaaccgttta acaatatatc
     7501 agaaaagatg ataattattt caattatttt tattaaattt cataatgatt cgtataatat
     7561 tcgcattaaa acatcacaat actgatacac aacaaaaaga gcagtacact tgtcatgcac
     7621 cactctctct taaaatatat ctaattatgc tttataaatg aattaccgga aatcaaaata
     7681 catcttgcct aatactaaca acatttaaag tgatgctatt agtcaaaatg tcttttaaaa
     7741 taactgaagt acttctttga atgagaatat acctgtcaat attgtaacaa gtactgcaac
     7801 gataccaaag atgaacatcc aatttgggtg tttataatca ccgacaattg atttcttttt
     7861 acttgcaatc aaaattgcac ctaacgtaat aggtaagatc catccattta tcgcgcctgc
     7921 tataattaaa aggctgattg gttttccaat aaataagaaa atcattgttg aaataacgat
     7981 aaacacaatc acaatcaaat tacttctttc gttaagtgat ttatgaagtg tttttaaaaa
     8041 tgttgcgctt gtgtatgctg agccaattac tgatgacata gctgcagcaa ataacacaat
     8101 accaaaaata ttctttccaa ttggtccaat tgcgtgttca aaaactgacg ctggtggatt
     8161 ttcagaactt agtgtcacac ctgttacaac aactcctaat accgctagga atagtaacgt
     8221 tctcataata cctgtagtta aaatacctgc aattgctgat tgatttacaa atggtaaata
     8281 ttgcttacct ttaatgccag agtctaatat acgatgtgca cctgcaaagg taatataacc
     8341 acctacagtt ccaccaacta acgtaatgat tggcaagact aatttcattg gatgttctgg
     8401 cgcaaatgta tgcacaaaag catcaccata aggtggatta gaaacaaaca tcacatatgc
     8461 cacaactaaa atcatcacaa taccaagaat cattgaaaca acgtccataa ttttttggcc
     8521 acttttactt acaaagatta atattgcaaa gattgcagta atagctgcgc cccattttac
     8581 atctaatcca aaaattgcat ttaaacctaa accggcacca gcaatattac caatattaaa
     8641 agctagacca ccaaatgcaa tcaatattga gataacagta ccaagcccag gaacaacttt
     8701 atttgatatt tcttgacctc ttaaaccagt tacaactaat atgcgccata tattaatttg
     8761 tgcaccaatg tcaatgatga tagacagtaa tatggcaaat gcgaaacttg caaaaaattg
     8821 tgatgtaaat actgctgttt gcgttaaaaa tgctggccca attgcagaag ttgccatcaa
     8881 aaatacagaa cctaataata accttttatg attttttgtg aattcaaagt cactttcttt
     8941 tttaagcttt aaatttctcc ccatttttta gccccctata aggattgaat atcaatgcct
     9001 tctttcatta aaatttctct aattttcgaa acaaataata aagcatgttc tccatcacca
     9061 tgcacacaaa ttgtatctgc ttgtaacgtt acttccttat tgttttttga aataacttta
     9121 ttttccttca ccatcttcaa aacctgctta agtgcttcgt cagtatcagt aatcacagca
     9181 tcactttctt ttctactaac gagctgccca tcatcttcgt atcgtctatc agcaaacact
     9241 tcagaagctg taattaatcc gacattcttt gcttctgaaa ttagatatga atttgctaat
     9301 cctactagca ctagtgatgg atcaaagtca taaacagctt gtgctataac acttgctatt
     9361 cctctgtctt ttgcacccat ctgatacaat gcaccatgcg gtttaacatg attaatttta
     9421 acttgatgaa tgcgacaaaa cccttgtaat gcacctaatt gataaatcat caaattataa
     9481 atctcctcgt tagagatatc tatatttcgt ctgccaaaac ccttcaaatc aggtaaacca
     9541 ggatgtgcac ctactgcaac attatgtgct ttggcaagtt ttaccgtttc attcattaca
     9601 ttttcatcac cagcgtgaaa accacaagca acattcgcac ttgtaattaa cggaataatt
     9661 tgatgatcac caccaaagga ataatttcca aatgcttctc ctaaatcaca attcaaatca
     9721 actcgcatta taattccacc cctttaacaa tttgatgttt ttctaaaaat ttaatatcaa
     9781 catcttttgc atctccatca cgatacagtg gataatttaa aactgcatat aaaaaatcgg
     9841 cagttgtaga aaatccatct atcaccattt catctaaggt ggctttcaat ttatcaattg
     9901 ctgaacctct atcatgagat tttacaatta ctttagctac taaagaatca taatatggtg
     9961 aaacttgata accgtgatat agtaaagaat cgactcgcac attaaagcct tgtggtaaat
    10021 gtaacgctgt cactttacct ggtgttggtt gaaatttctt ttcaggattt tcggcattta
    10081 ttctcgcttc tatcacatga ccgttaaatt gaatatcgct ttgtgaaaaa ggtaaatgat
    10141 tatgttccaa taaatacaat tgtgctacaa ccaaatcacg ttctgctcgc atctctgtaa
    10201 cagtatgttc aacttgaatt cgagcattca tttcaataaa gtaatgtgca gtatcagtta
    10261 ctaaaaattc aatcgtacct gcacttctat aatttgctgc acgtgcaact ttaacagcat
    10321 cgttacatat ttgttgtcgc ctttcttcag ttaatgctgc acaaggagat tcttcgatta
    10381 atttttgatt tttacgttgt acagaacaat cacgttcccc taaatgtaca taattatcct
    10441 gcccatcccc cataacttga acttcaacat gttttgcaac aggtataaaa gcctcaacat
    10501 aaacacgatc atcatcaaag tatttttttc cttcactttt agcttcttta aatgcctttt
    10561 ctaaatcttc agctttcttt acaatacgta tacctttacc accaccgcca ctggcagctt
    10621 tgataacaac tggataaccg atgtctttgg caagcttctc aatttcagac acatgattca
    10681 cagcaccatt tgatcctgga atcacaggaa cacctgcatg atgaactgtt tgtcttgctg
    10741 ttattttatc ccccatcatt tccatcgttt ttttagtagg ccctataaac gctatgcctt
    10801 gttcctcaac ggtttgagca aattttgttg attctgataa aaagccatat cctgggtgaa
    10861 ttgcattagc accagtgatt tgtgcagcag atatgatgcg gtcaatattt aaataactat
    10921 ctaaagcatt agcttcccca atacatatag cttgatctgc taaatgtaca tgcaagcttt
    10981 gctcgtcccc ttttgcataa actgctacag tttcaatccc atattctctg caagctctta
    11041 taatccttac tgcaatttca cctctgttcg caattaaaca acaaagcatt tacttacccc
    11101 ctttacttaa tacgtaccaa aacttggtcg tattcaacat ttgtgccgtg atcagctact
    11161 atttcagtaa tttctccagc aacatctgtt gttacctcgt ttaatacttt catcgcttca
    11221 acatatccta taatatctcc cttgttaact ttgtcaccga ctttcacaat tggttcagtt
    11281 aattctttac tgtcttgtaa aaagaatgta cctaccattg gtgatttaat gtcatgataa
    11341 tcatttgtcg aaacatcgga gttatcattc gcttttgaag ctgtcaaatc attattgttc
    11401 atactttgat ttgattgatt actgtgtgca gccaaatgat tcgagtcagt gaagtcaatt
    11461 tctatttcat cttcaaaatt tttatattta aatttcttaa catcattttc cttcactaat
    11521 ttgattattt gttcgatttt ttcaatattc attttacaaa tcccctttta aaattgttgc
    11581 taattttttc gaagtatgtc gtaagctaga tgtatcaaaa attggagtct tttcatgact
    11641 cttaagaatt tcattaaaca gagacatttg ttcccgattc ttatctacag cttcttggaa
    11701 tgatatccat ttaaattgaa ttgtatcttg tggtttcatc tgtgctaact ttggcagatc
    11761 aaatttgcat acagttgcaa ttttggtata accacctatc gtttgtttat cattaagcag
    11821 aataataggt tgaccatcat ttggtacttg aacactgcca agagcaaccg gttcagaaat
    11881 gatatctgct tgattaattg gtgcaacgct gtcaccttcc aaacgatacc ccatacggtc
    11941 tgattgttca gtaattaaat atggatgatt tacaattttc gctctagcct cttcagaaaa
    12001 tgcctcgaat tgaggtcctt gaagaatgtg tataatatta ttttctggca ataaatcgtc
    12061 ctgtaaatga atagtctttc caatgttttc tttaaagtca ttatttattt tcactgttat
    12121 tacatcatta gctaataact ttctaccttt gaatcctcct atactgcttc gggtatgtgt
    12181 tgcataacat tcagcaattg gaggtacgtt gatagtatga ccaaaagtaa gataaccgcg
    12241 tgcacctttg gttatagcac ctatttttaa aatgtcacct ttctcagcta atatgacaga
    12301 attcattgat atagttttat tatttagcga ggcattaaca ctaccaccag ttataacaaa
    12361 tgtattttgc gtattaaatt gaatggtagg accaatcaaa gtatattcaa tcgctggacc
    12421 atcattgtta attaatgact gcgcaacctt aaaactaaat tgatccatgg cacctgcgcc
    12481 tgaaaatcca atatgttcat aaccttttct tcctagatct tgtaccgttg aaaagagacc
    12541 tggttgtaaa atcttaattg acattttcga tcaccaccca gtcatcaaca ttaaagttgc
    12601 catctgatat atctctttcg atttgtataa attcctgttc atctattgca taaaattgta
    12661 tccattctcc tgcttcatac attgacattg gttcacgctc gctgctaaat acttttaacg
    12721 gtgtgcgtcc aataatttgc catccgccag gagaatctga tggatatagt cctgtttgat
    12781 tattcgcaat acctacagaa cctgcatgaa tttttaacct tggctgatta cgtctaggtg
    12841 tatatagttg ttcatcaagt ccgcctaagt atggaaatcc aggcataaat cctagcatat
    12901 atattaaata aggtttactt gtatgttttt caataacttg ctcaacagtt attcgattat
    12961 gctttgctac ttcttcaata tcaggtccat atgtaccacc atattgaaca ggtattttaa
    13021 taatacgatt ggtttgattc acagcatgaa catttttttc attaaatttg ttaagttcta
    13081 aattttcaat taatttagaa ggtgttatag cttgttcatc aaaatatatt agaactgctc
    13141 gatacgaagg gacaatatct tgaatttcta atatttcttt ttctcgtatc caccgtacca
    13201 ttgctgtgac atttcgatat gtctcttcgg atattttatt ttcaaaataa atcataattg
    13261 tctgctcgtt aataaatctt acatccactc taaatccccc tttgtattgc aataaaccag
    13321 tattgaatac cttttcattg tatcattgag aagcacaagt tgtttaataa gtaattcaaa
    13381 tcgcatgcaa taacaaatga taatcaattt gttttctcgg aaatattttg cttttttaaa
    13441 atggagtagt taatattgaa actcttacac taatttaggt tctgctatca atcggtctga
    13501 tggaaaattt ttacttttca tctgtccgat tttttgattt tgaatataaa aaagcacgac
    13561 cgaagtatca ttaacacact tcaatcgcgc aattaaacaa tctatttgac catttattgg
    13621 atattaacaa tttttacgtt catttcgcca ccattaggta gtggaacacg aacttcatca
    13681 tctaaacctt taccaattaa cgctttagcc attggtgatt catttgaaat cttaccatta
    13741 aatgcatctg attcagctga accaacgatt tgataacttt cctcttcatc acctggtaat
    13801 tctacaaacg ttactgtttt accaatttta acaacgttgt tatctccagt atcttcaatg
    13861 attaatgcat ttcttaacat atgctcaatt ctttgaatat cttgttcgat gaatccttgt
    13921 tcatcttttg ctgcatcata ctcagagttc tctgataagt caccaaatga acgtgcaact
    13981 ttaattttct ctacaacttc aggacgctta actgttttta attcttcaag ttcacgctct
    14041 aatttttcaa aaccttcttg agtcattgga tattgctttt gattttccat attgtcatct
    14101 tcctttactg aattatacta ttgcttgcta actaaagact gaatttttgt tgtcataata
    14161 tctattgcaa ctttattgct cccaccttca ggaataatta tatcagcata tttcttagtc
    14221 ggttcaataa attggtcatg cataggtcta acaacactta aatattgatt gataacagag
    14281 tccattgaac gcccacgctc tttagtatct cgtgttaaac ggcgtaatat tctcaagtct
    14341 gcatctgtat caacatatat tttaacatcc atcatatcac gtaatacctt attttctaaa
    14401 gcgaaaatac cttctacgat aataacatct ttaggtttaa aatcaatggt aatgtcactt
    14461 cttgtatgac tagcataatc atatgtcggt acttctactg ctttaccatt tttcaagtct
    14521 ttaagatttt caattaataa atcattatcg aatgcaaatg gatggtcata attggtttct
    14581 aggcgctcgt cgaaagtcaa gtgcttttga tctttataat agtaatcttg agcaagtaaa
    14641 gcgacactat gaccttctaa gtttttcata atttcgttag ttacagttgt ttttcctgag
    14701 ccagatccac cagctatgcc aatgattgta gtagctttca ttagccaatt tcctttctca
    14761 tcatgttgtt tggatatatc gggcgatcca ctttaatttg aacgatttgt aatggatggc
    14821 gcgccgcgtc taagctgtta ccttcttcat cataaattgc ttctactact tgtgtaaatg
    14881 tttcaatttc tggaccaaag aattctattt cttgacctgg tttaaagtta tttcgttgtt
    14941 gaatagtcgc aatttttgta tcttcattat aatctaatac caaaccgcaa aaatcaaatg
    15001 gtgatttttt agattgttgt tgaccaaaca tctgttcttc ataaccaggt gttccttcaa
    15061 aaaatgctgg cgcagtatct ctatttgcac acttatctaa ttcaattagc cactctgggt
    15121 taattttgaa gttttcaggg tcagctgcat aggcatcaat aactttacga tatactgaga
    15181 ctactgtcgc aatataatga attgatttca tacgtccttc aattttcaat gaatctacac
    15241 ctatatccat catttgagga atagattcga ttaattttaa atctttcgga ctcatcgcaa
    15301 atggtttaac ttcaccttga tcataaaata catctagttc accactatca tcaacttcta
    15361 atagttcata atcccaacgg caactctgac agcaaccgcc tctgttggaa tcccttgcag
    15421 tcatatgatt acttaatgta catctacctg aataggcgat acacatagca ccatgaataa
    15481 atgcttcgat ttcaatatct actttttcct tcatttcacg catttccatc gcgccggtct
    15541 cacgtgctaa tacaacacga tccaatcctt cttctttcca atattctaca gctttgtaat
    15601 tagaaagtga ttgttgagta gataaatgaa tttcaagttt tggcgcaact tctttacatg
    15661 tttcgataat taaaggatct gcaacaatga tacctgtcgc accagtcttt tccaaattac
    15721 gcaaatatga ttctagacct tcaatattct catcatgtgc aataatattt gtcgtaacat
    15781 aaattttggc accgtaacgg ttcgcaaatt caacaccttc agctatttct tccatcgtga
    15841 aattatcagc atttgaacgt aatccatatt cttgaccacc taaaaatacg gcatcagcgc
    15901 cataatgtac tgctattttt aacttttcta agtttccagc aggtgctaat aattctggtt
    15961 tcttcataac tgttttagga gttgatttaa tctcttctat tgtcttcatg attaacctcc
    16021 ttaatatact gtttgtttat ataagaaacc ttcgtcaaat ggtcgatgat caggttgaat
    16081 ttcttcaatt gggtccatca acataaattt ctcatcttca tagatttcag gatcttcatt
    16141 gtacaaatct atcgcttgac gatactgttc cgttaccaca ttaatatatt cttccgtttg
    16201 tagaatacca tcgattttaa atgaatctat acccgcctca aaaaatggtg ctaattcttc
    16261 aattaaacaa atgtcgtttg gtgacataat gtgcgtacca ttgtaatctt cgtaaactgg
    16321 gtaattattt tgtctttctt catcataaag taataaagat tgttcatcat tgcgacgttc
    16381 aattttcatt tgacgatctt ggaacgtata ataattgcct agtagcatac gctttgattg
    16441 gaacatacaa gtcattcctt gaacttgcac ctcaatttcc acatttgaat tttcttttat
    16501 attaataatt tcatccaaat tcagctcacg tgctaagaca gctcttgatg cacctctttt
    16561 accccagtaa ttacattgaa aatgattagt tactaacgtc tctgcattcc aatgaagtgg
    16621 tattggattc tcttgcgcct tcacatacat tactactgct ggatccccga aaataattct
    16681 gtcaactcgt atttcatgta aaaaattaat ataatcttct acagcatcta aatgataatt
    16741 atgaaataat ccattcaccg ccgcatatac ttttttatcg tttttgtgag ctaatgcgac
    16801 agcctctgtc atttgttgtc tattgaattc ccctggaagt cttaaaccaa acttttgctc
    16861 gccaattaca aaagcatctg cacctaaatc aataagtgtt tccatatggc ttaatgactt
    16921 gggtgtgaca agtaattctg tcatagtcat tctcctttaa ttgaaatcgc taatccatcg
    16981 tctatattta aaaaattcgt tgtatatcct ggttgcttta ttaaccactc attataatct
    17041 tgaacctttt taaccatttg tcttacattt ctcgatctaa caatcccaat atccgataca
    17101 aaaccgtgat ataaaacatt atctgtaatt acaagacctt ggtgctttaa aagtggtgta
    17161 tatatttcaa aaaacttctt cgattgggct tttgctgcat caataaatat catatcataa
    17221 actttgtcat ttacattttc aaattgatct aatgcattac cttcaataat tcgaacctgg
    17281 ttttcaaaat gataagtagc tagattttgt ttagcatatt gaatcatcgt ttcattacgc
    17341 tctatcgttg tgacatgaat gtcatcagat atagaagcga attgcataga actatagccg
    17401 attgctgtac caatttctaa aatattttta acattattca tacgaattaa ttgcttaatt
    17461 aaatctaatg ttaaacgatc tacgattggc acttcattta cttcggcaaa ttcacgcaaa
    17521 acttcgattg aactattttg atgttgatgt aaatctatta aatatttttt atttaggtca
    17581 tccatgtttt aaacttcctt tatgtaaaat aagtcaatat gattatgaca ataaaataaa
    17641 tcagccttca caattgatta taattttgcc aaccaattaa atgactgatt tcgtgttaga
    17701 cgcaaagcta ttttatttat agaagcgaat cattcatata aaatttaact ttagatattt
    17761 taccatattt tcaataaaat tataagcgtt aattatttat acattgcttg acctaaaaaa
    17821 atactcttgc ctccccatct taaggttagc aagagtaaaa tctttttaat tattcttcca
    17881 tttcagtatt tacaacttct tcaatcatgt cccattcttc atcagtttcg attggtacta
    17941 acttaccacc gtcacctgac tcatctggtt cattgatcat tggtacaagc tcaatcatat
    18001 cgtcttcatc tgattgagca ccttcttcag ctaagataac atactctttt ttgaattcag
    18061 gatgataaaa ttctaaaact tttcggtata aaacttcatt tccttcttca tcgaataaag
    18121 ttaataattc ttcttcgtta ttaatttcta gttgtgaatc atgattatgt tcagtcatag
    18181 taaaatctcc ttttaatgta gtgaatctaa atagccttgt aaaataaata ccgctgccat
    18241 tttatcaatc acttgttttc ttttttgtct tgaaacatct gcttctaata atgatcgttc
    18301 agcagccatt gtgcttaatc tttcatccca catcacaatc tcaatagaag gataagcttc
    18361 taataatttt tctttatatg ttaacgaagc ttcgcctcga aatcctattg aattattcat
    18421 gtttttaggt agtcctatta cgactgtacc cacattatgt tttttaataa tgtctactaa
    18481 ttggtcaata cctaattcat tattttcttc attgattcgg agtgtgtcta atccttgtgc
    18541 cgtccaaccc attatatcac taattgcaat tcctaccgtt ctactaccga catcgagtcc
    18601 taaaatttta tgttgtaaca taaattattt attttgctct tttaaatagt aagaaacaag
    18661 ctcttccata ataacatctc tatcaatatg acgaatttga tttcttgctt cattttggcg
    18721 tggaatatac gcagggtcac ctgataataa ataacctaca atttggttta cggcattata
    18781 tcctcgttca tctaatgttc gataaacatt atttaaaaca tctcttacat cttgtgttgg
    18841 aagttcttca tagtcgaatt tcattgtttt atcaaagttt tccatttgcg acactccttt
    18901 aattacaaat ataactcact atcatcatac aatattatgg ctttaaatta tagattttta
    18961 atgtaatctt taataaagct taatgatttt gagatatttt caggttgtgt accgccacct
    19021 tgagccatat ctggacgacc gccaccttta ccaccaacga ttggtgccat ttgtttgata
    19081 agatcaccgg ctttaacatt atttgttaaa gatttaggga cagttgcaac catcgatact
    19141 ttatcatcaa cattacttgc aagaatgata attgtatctt gtagtttaga tttaaaatcg
    19201 tccattgtcg agcgaattgc tttcgcattt ggtacatcca cttcagtgac caatacttta
    19261 tagccattga tttcttcaac ttgatcttca atattaccca ttttaagtga tgtgatttct
    19321 ttgtcacgtt gctctaattg ttttaataat gctttttctt catcttgcaa ttgtgttaac
    19381 ttatcgacta cttgatcatc agatttcact ttcagctgtg atttcatcgt attaaatttc
    19441 tcttgaatat cttctaaata taagaacgct gctttacctg ttaatgcttc aatacgacgc
    19501 acaccagctc ctgtacctga ctcacttact attttgaata agccaatttc agaagtattg
    19561 cggacatgaa taccaccaca taattcaatt gaaaatggtg ccatatttac tacacgcaca
    19621 acatcaccat atttttcacc gaataatgcc attgcaccca tttctttagc tgaagcaata
    19681 tccatttctt gaatgttaac gtcaatacct ttccaaattt cttcatttac taagcgttca
    19741 acttgatcaa tttcatcatt agtcattgga ccaaaatgag agaaatcaaa acgtaaacga
    19801 tctgcttcta ctagtgaacc agcttggtta acatgatcac ccagtactga tttcaacgct
    19861 gcatgtaata aatgtgttgc actatggttc ttttgaatgt cacgtcgatc attttggttc
    19921 acttcagcag acactgtagc gccaacattt acttggccaa attgtactac tcctttatgc
    19981 aagttttgac catttggtgc tttggttact tcactaacag caatttcaaa attgtcatta
    20041 taaacaatac ctgtatccgc aacttgtcca ccactgactg cataaaatgg tgtttccgtt
    20101 aacatgaagt atactgtttc acccgcttca acttgtgaaa cttcttcacc attatatatc
    20161 aagtgtgtta gtgttgtttg agctgtcgca gtatcataac caacaaaagt acttgcaaat
    20221 gtaatatttt tcaatacttc actttgaact tgcattgatt gagaattttg acgtgcttga
    20281 cgtgcacgat cacgttgttg ttgcatttct gactcgaatg ttgtcatatc aactttcaat
    20341 cctgcttgca ctgctatttc ttcagttaat tcaattggga acccatacgt atcatacaat
    20401 ttaaatgcat ctttcccact aatttcattt gttgtcactt tagctttttt aattaattca
    20461 tttaaaatcg ctaaaccatc ttctaatgtt tcatggaatc gttcttcttc agactttata
    20521 acacgcttaa tgaaatctgc tttttcctta acatttggat aatatggttc cataatgtct
    20581 gcaacaatat caacaagttt gtacataaat ggctcattga ttcctaacgt ttgactaaaa
    20641 cgaacggcac gacgtaacaa tcgacgtaat acataacctc taccttcatt ggcaggtaat
    20701 gcaccatcag aaattgcaaa tgcaatcgta cgaatgtggt cagcaattac tttaaatgcc
    20761 acatcttgtt cgttgtttac taaatattgt ttacctgata ctttttcgat ttcattcatt
    20821 ataggcataa ataaatctgt ttcatagtta gtacgtacat tttgagaaac tgaggccata
    20881 cgctcaagcc ccatgccggt atcaatgttt ttattaggta atggtgtgta actatgatct
    20941 ttattatgat tgaattcact aaatactaag ttccatactt caagatagcg ttcattttct
    21001 ccacctggat acatttcttc tgacggatcg tcttgtccat atgcttctcc gcgatcatag
    21061 aaaatctcag tattcggtcc tgaaggccct tcgccaatat cccagaagtt accttcaatg
    21121 cgaataatac gactttcttc aagtccaata tctttatgcc aaatgttgta tgcttccata
    21181 tcttccggat gaatcgtaac gtacaattta tctggctcca tacccatcca tttatcactc
    21241 gttaaaaatt cccaagcaaa ttcaatcgct tcttgtttaa agtaatcacc aattgagaag
    21301 ttacctaaca tttcaaagaa tgtatggtga cgcgctgtga aaccaacatt ttcaatatca
    21361 tttgtacgaa tagctttttg agagtttaca attcttggct ttttaggtgt ttcacgtcca
    21421 tcaaaatatt tctttaatgt tgctacacct gaattaatcc ataataatgt atcatcatca
    21481 atcggtacta aaggtgctga aggttctacc atatgtcctt tttcaacaaa gaaatcaaga
    21541 tatttttgtc taatttcact cgcttttaac tttttcatca tttacacatc ctatttactg
    21601 tttttaaaat ttcattccat aaaaattgat gacacagata atcgatttaa ttataccgtc
    21661 gacaaaaaat ttgcatatgt tttatcttat aaaacataaa aaacgcccat cctcaaaagg
    21721 gacgaacgtt atcgcggtac caccctagtt ataagcatat tatgtacgct tatcacttta
    21781 aatcgactat acagttgtgc ataaagtagc gttcactaat gtttgttgta ctttacacca
    21841 accagtacat ctctgataaa caaatcatta actactcatc tttatacgaa tttaattcta
    21901 ttttagttac atttacgctt gttgtcaacg ttctataaag tcatacggcg tgatttctcc
    21961 catgttaatc attgggtcaa ttttaaacat tgtagcttcc gttaatacat ttgtatctgt
    22021 ttttgttgaa tcagacgtaa cttcttcgct atcatttgat gacaatgaag cttctacttg
    22081 atcatctatt gtcgtttgtg aagctcctgt atcattagtt gctgtgtttt ccagcatttc
    22141 ttcatcttct gaattaaaat aatttttcaa caatgtacat aattgtgtta aacgcgcttg
    22201 accatttgtt ttcaatccaa tatcaaatgc ttccggatca ccaagtaaaa ctaaactcgt
    22261 tttcgctcta gttaaaccag tatataatat cggtctttgt aacattctaa aatactgttt
    22321 aacaataggc atgataacaa taggaaattc tgaaccttgt gatttatgga ttgatgtaca
    22381 atatgcatgt gttaattcca tcatatcttg tttcgtaaat gtaatttcat taccttcaaa
    22441 atccacaaca agtacatctt tattaagggc attttctttc gcccaaaaaa taccaacaat
    22501 aactcctatg tcaccattaa atatgttatc atttggccta ttaacaagtt gtaatacttt
    22561 gtcacctttt ctaaagacta catcaccaaa ctcaatttct cgtgtgtctt tctttttagg
    22621 gtttaaaata tcttgtaaaa cttgatttaa acgtttaata ccagcatttc ctttatacat
    22681 tggtgcaagc acttgaatat cagccatagt atacccttta ttaacagcac tagtaactac
    22741 cttctcaaca actgttggta tttggtttgc ctgacagtta ataaaacttc tatcatgaaa
    22801 acgctgtgta atatcaattt tctgacccaa tttcattcga tgtgctaatt ctataatgct
    22861 tgaaccatct tgttgacgat atacttcagt cagattaact cgtggtatag ctttcgattc
    22921 aattaaatct ttaaatactt gaccaggacc tacagaaggc aattggtcct catcacctac
    22981 aaatatcaat tgtgcatcta aaggaactgc acttaaaaat tggtggaaca accaagtatc
    23041 taccatagac atctcatcaa tgattatgag tcgtgcgttt atttcatttt ctaatatatc
    23101 ctctggcttt gtgtcttgat tccaacctat taaacgatga atcgtcatag cttctaatcc
    23161 agttgactct tgtagtctct tggacgctct tcctgttggc gctgctaata caactggata
    23221 atcatcattg acataatcat cataatctaa tgataagcca tgaatctcag catataattc
    23281 tacaatgcct ttaattactg tcgtttttcc tgttcccggt ccaccggtta atagcatcac
    23341 cttagaattg atagccgttt gcaaagcttc tttttgtgaa gctgcatagt tcacttgatt
    23401 cgcatcttct atttcaccaa tatgcatttg taaatctgac tgttcaattt ccgtaagttt
    23461 atttgtatgc gtctttattc tgaataagtt ttgaacactt ttgatttcag aataatacaa
    23521 acttggaatt gcaacttgtt cattgtcaat aattagtcgt ttttcctcat ttaagtattg
    23581 caacatttcg tctaattttt caggttcgat gacttcttca tcttgataat ttaatacatc
    23641 aacggttaag tctataacaa cattgatagg caaatatgta tgtccctgtt taatacattc
    23701 ttcttctaac gtatagagca acgcagcttt taatcgttca ttatcgttat aagcgatacc
    23761 aatatttcta gcaagttgat ctgctttatt aaaaccaata cctttaatat cataaatcaa
    23821 ttgatatgga tttcgatcta aaatagtcag tgtatcaccg agataaaact gataaattgc
    23881 cattgaaagt ttaggaccaa accctaaatc atgtaaacga atcattattt tttcagattc
    23941 ttgatttgct gaaatttgtt ctgcaatttg tttctgtttc tttttagata atcccgaaac
    24001 tttttctaga actgaatggt catctaatat atcatttatc gcattatcac ctaatgtatt
    24061 aacaatattt tgagctgtct ttttacctac acctttaaac aaatcacttg ataaataact
    24121 tataattgct tctttcgttt gtggcatttc tttttcaaaa gtctctgctt ttaattgttt
    24181 accataacgg ggatgatcaa caacttgacc tttaaatgtg tagacatcgc cttcaacaat
    24241 attcggaaga aaccctacaa cagttggcat tgtatcaaag tcttcatttg tttcaatagt
    24301 atctacttta agcactgtat aaaaattatc actgttttga aacaatatcg cttcaacagt
    24361 acctttgatc attgaataat caaatagtgt agggtctgac atgttattcc tcctctttca
    24421 ttttagtgaa tgttttcagc gcatgctgac ttaataagtg tttagggtcg atagtcacag
    24481 cttctttaaa atgagttatg gcttcatcaa tatcttcatt tttcataaat aacgctaaac
    24541 ctaaattgta tcttgcatca acatgatttt tatcaatcgt taatacatgt ttaagttgag
    24601 ttatggcttc attaaacatt tctaattgac ataatacaat accatattga aattgaactt
    24661 ctgcatcttt atctttatct agttccgcag cagtcattaa atacggcaat gccagcttaa
    24721 atgattctaa ctgattaaac gccataccga tcatataatt acaatcaact tgttcaatct
    24781 ctgtttgtaa tgcttgttga tataatttaa tagcttcttg ataacgttgc tgattataat
    24841 atacatttgc tagattataa aatacgacgc cattcttcgg atctattgtt aaagcttttt
    24901 ggaaaaaacg ctctgccttt tcaatctcat tcgcatcagc aagtacgata ccggcattaa
    24961 tataattttc aataattgta ggatcttctt cgatatttcc gaacaatgct tgtaacgctt
    25021 cttctatttt tccattttgt atgtattgat aaattgtttg ttgatctatc atttacgaac
    25081 ctcatttctc atcaattata acatcttgat aaattgtatg tctcgaatca cttaacaacg
    25141 aataaaatat aatctaatat catcttcatt catgaaaaag cgggaatgga atagaaatgc
    25201 taaagaacca ttaacggttt gttatgtaat ggttcttcca cattagccac cactattatg
    25261 tacttaaaaa taggaataca taattagatt catgcatagg gagtgggaca gaaatgatat
    25321 ttttacaaaa ttaatttgtt taccccaact cgcattgcct gtagaatttc tttacgaaat
    25381 tctctatgtt ggggccccgc caacttccat tgcctgttga atttctttac gaaattctct
    25441 gtgttggggc cccgccaact tccattgcct gtttaaattg gggttccaat ttctctgtgt
    25501 tggggccccg ccaatataac attgtagagc ctaggacatt gtgatgtccc agactctatc
    25561 atcatgaatt attctcatca aaaactgttt ttcgtcattt ccaacgctaa aacttcaaat
    25621 aagtaattta ttgttgccat tgtttataca acataattta attgaccttc atttttgaac
    25681 acatcgtcaa ttgttgcacc accaagacac acatcgcctt gataaaacac aactgcctgt
    25741 ccaggcgtga ttgctcttac tggctcagca aaagtaacac gtagtgcatg gtcgttttca
    25801 cgtttcacaa aaactttcgt atctttttgg cgatatctaa atttagctgt acattcaaaa
    25861 ccttgatcta agtcattatc ttctggattt acaaatgaat agtctgaagc aattaagtaa
    25921 tcactgtata atgcatcgtg atggaatcct tgttctacat ataaaacatt atcttttagg
    25981 tttttaccga caacaaacca aggatcgcca tctccaccta tacctaaacc atgtctttgt
    26041 cctattgtgt aatacatcaa accactatgt ttacccattt tcttaccatc aagtgttatc
    26101 atatcacccg gttgcgcagg tagatattga gataaaaatg ttttaaagtt tttttcgccg
    26161 ataaaacaaa tacctgtaga atcttttttc ttagcagtaa caagtccttg ttcttcagca
    26221 attcgacgca cttcactctt ttcgatgtcg ccaatgggga acatcacttt tgaaagttgt
    26281 tgttgagata attgattcaa gaagtatgtt tgatctttat tattatctac accacgtaac
    26341 atttcaacat gaccatcttc atgacgatgt atgcgtgcgt aatgtcctgt tgctacataa
    26401 tctgcaccta aattcatcgc atgatctaaa aaggctttaa acttaatttc tttattacac
    26461 ataacgtctg gatttggagt acgacctttt ttgtattcat ctaagaaata cgtaaagact
    26521 ttatcccaat attctttttc aaaattaaca gcgtaatatg gaatgccaat ttgattacac
    26581 acttcaataa catcgttgta atcttcagtt gcagtacata cgccattttc gtcagtgtca
    26641 tcccagtttt tcataaatat gccaatgaca tcataacctt gttcttttaa gacgtgggct
    26701 gttacagaac tatctacacc gcctgacata ccaacgacaa cacgtatatc tttatttgac
    26761 aattatgact cctccttaaa tttaaaatat attttatgaa tttcagctac aattgcatta
    26821 atttcatttt cagtagtcaa ttcgttaaaa ctaaatcgaa tcgaatgatt tgatcgctcc
    26881 tcatcttcga acattgcatc taaaacatgc gacggttgtg tagagcctgc tgtacatgca
    26941 gatccagacg acacatagat ttgtgccata tccaacaatg ttaacatcgt ttcgacttca
    27001 acaaacggaa aatatagatt tacaatatgg cctgtagcat ccgtcattga accatttaat
    27061 tcaaatggaa tcgctctttc ttgtaattta actaaaaatt gttcttttaa attcattaaa
    27121 tgaatattgt tatcgtctcg attcttttct gctaattgta atgctttagc catcccgaca
    27181 atttgtgcaa gattttcagt acctgcacgg cgtttcaatt cttgttcacc gccaagttga
    27241 ggataatcta atgtgacatg gtctttaact aataatgcac cgacaccttt tggtcctcca
    27301 aacttatgag cagtaatact cattgcgtcg atctcaaatt cgtcaaactt aacatcaaga
    27361 tgtccaattg cttgaaccgc atcaacatgg aaatatgcat ttgtctcagc aataatatct
    27421 tgaatatcat aaatttgttg cactgtgcca acttcattat ttacaaacat aatagatact
    27481 aaaatcgtct tatctgtaat tgtttcttca agttgatcta aatcaatagc acctgtatca
    27541 tcaacatcta gatatgttac atcaaaacct tctcgctcta attgttcaaa aacatgtaac
    27601 acagaatgat gttcaatctt cgatgtgata atgtgattac ccaattgttc atttgctttt
    27661 actatgcctt taattgccgt attattcgat tctgttgcgc cactcgtaaa tataatttca
    27721 tgtgtatctg caccaagtaa ttgcgcaatt tgacgtcttg actcatctaa atatttacgc
    27781 gcatctcttc ccttagcatg tattgatgat ggattaccat aatgcgaatt gtaaatcgtc
    27841 atcatcgcat ctactacttc aggttttact ggtgtggtcg cagcataatc tgcataaatt
    27901 tccatgtttg gacactcctc acaattttat caatgttcca ataatagcac cttacatact
    27961 atttttctac ttttctgttt aactttattt ataatgtttt taattatatt ttaccatttt
    28021 ctacacatgc ttttcgatag gcttttttaa gtttatcgct ttattcttgt cttttttata
    28081 aattttagta tttgcagata ttttttcatt tgtaaaatgt aacgtactat tattttggtt
    28141 atgagcaatt taatatttat ctggttattc gattggtata cttcttatat cataaaaaag
    28201 gaaggacgat acaaaaatgg cgattaaata ttcagcatta aaccttgtcc ctattcgaga
    28261 aggtgaagat gaacgaacag caattaatga tatggttaag ctcgcacaac atttagacga
    28321 attatcatat gaaagatatt ggattgctga acaccataac gctcccaacc tagtaagttc
    28381 agcaactgct ttattaattc aacatacgtt agaacatacg aaacacatac gtgtaggttc
    28441 tggaggaatc atgttaccta accatgctcc attaatcgtt gcggaacaat ttggcacgat
    28501 ggcaacatta tttccaaatc gtgtcgattt aggattagga cgtgcacctg gaacagatat
    28561 gatgaccgca agtgcattaa gacgagatca acatgatggt gtttataaat ttccagaaga
    28621 ggtttcatta ttacaacaat atttcggccc cgctcaccaa caagcatatg ttcgtgctta
    28681 tccagcagta ggtaaaaatg tgcctttata cattcttggt tcttcaacag attctgcaca
    28741 tttagctgct cgcaaagggc ttccatatgt gttcgctgga cattttgcac cccaacaaat
    28801 gaaagaagct atcgaaattt acaaaacgtt atttgaacct tctgatgtct tagacgaacc
    28861 ttatgttatt gtatgtttaa atacaatcgt tgctgaaaat gatgacgaag cacaatattt
    28921 agcttcatct atggcacaag taatggttag tatcactcgt ggcagaatgc agcccgttca
    28981 accgccaaca catgaactac aaaatatatt aacgccgaga gaatacgcga tggctatgga
    29041 aagacagaaa atatcattaa taggttcaga aaatactgtt caacaaaaaa ttcaagattt
    29101 tatggaaact tatggtgaag tcaacgaaat tatggcaata agttatattt atgataaaga
    29161 tatgcaatta gactcttatc gtcggttcaa gaatgttata aatcagataa ataaaaaaaa
    29221 cactttataa tgtgataaat aaactaagtg aaagtatgta tccatgatat taataaaaat
    29281 atacagtaac agcattctga atgaaagatg tctttattgt tcaatcattt attttagtaa
    29341 tgattcaaat tcgcttaaaa ttctaataca aatatgaaag cgccccttca actttacact
    29401 gtgtaagtgt ttatttgatg gggcgctttc aaaatattga aaagcatatc caaaatttaa
    29461 agaattttat ttctctttat cttcattttc ttttttctct tcgttattcg aacctgtata
    29521 ttcatttatc ttatctttta catttttaac ttgttcatta tcgctatttt taaatttttc
    29581 tactgcgtct ttagctttat ccataaaact catattaatc gctcctctta tatttgattt
    29641 gtttaattga acttattttt taagtttatc aattgcatca gttattttgt ttttagcatt
    29701 ttcaacaact tcttttgctt taccagtcgc tttatcttgc tgaccttctt tttctaattc
    29761 tttgttgtca gtaacgttac ctactgtttc tttaacattg cctttaaatt gatcgaactt
    29821 actttcgtct gccatagtga aacctcctta gatgtatata tttatatacc acaaaggagg
    29881 ttcgctaaac attataatat gaagttttta tgttatagta tagtatttat acgattaaat
    29941 ataaaacatg tatccgtcta aatcttcact tgtatctaca tattccgcta aatatttcaa
    30001 tgttgtatta tctaaaacat ctctcactgc atctctcatg cgaatccata gttgtttttg
    30061 cgcaggtggt tctgattcaa tactttcaac aaatgtaatt ggaccttcta acagtcttat
    30121 aatatcccct gctgagattt cttccgctgg cactcttaat tggtatccac ctttagcacc
    30181 gcgtacactt cgaattaacc ccgcatttct taaaggacct acaagctgtt ctaaatataa
    30241 atcactcaaa ttattttctt cagcaattga ctttaatgat atacatcctt gcccctcttt
    30301 tttagcaaga gaaatcatca atgtaagtcc atatctccct ttagtagaaa ttttcattgt
    30361 ataacctcac ttaattcgaa tattgatatt cccattttag cattttttga gttaagatag
    30421 tataagaaag gtgtgacaaa tgtgagtaca gaaccattag catcgagaat gcgcccaaaa
    30481 aatatagatg aaatcatttc ccaacaacat ttagttggac caagaggcat tatcagaaga
    30541 atggttgata caaaaaaatt aacttcaatg attttttatg gtccacctgg tataggcaaa
    30601 acaagtattg ccaaagcaat ttcgggcagt acgcaatata aattcagaca attgaatgct
    30661 gtaactaaca ctaaaaaaga tatgcaactt gttgttgaag aagctaaaat gtctggtcaa
    30721 gttatcttgt tattagatga aatacatcga ctagataaag ctaaacaaga ctttttatta
    30781 cctcatttag aaaatggcaa aatcgtcttg atcggtgcta caacttcaaa tccttatcat
    30841 gctatcaatc cagcgattcg ttcaagagcg caaattttcg agttatatcc tttaaatgat
    30901 gaagatgtgc gccaagcgtt aactcgtgca atagaggatg aagagaaagg tttaaaaaca
    30961 tatcaaccca aaattgatga agatgccatg gcctactttt ctacacaaag tcaaggtgat
    31021 gttcgtagtg cgttaaatgc attggaatta gctgtattaa gcgcagataa tgacaaagac
    31081 ggttatcgac atgttacatt gcaagatgct aaagactgtt tacaaaaagg tgcatttgta
    31141 agtgataaag atggtgacat gcattacgat gttatgagcg ctttccaaaa atctatccgt
    31201 ggtagcgacg tcaatgccgc tttacattat ttagcacgat taattgaagc tggagattta
    31261 cctacaatag ttcgacgatt acttgtaatt agctatgagg atatcggctt agcctcacct
    31321 aatgctggtc agagaacact tgctgctatt gaatcagcag aacgtctagg cttaccagaa
    31381 gctagaatcc cactaagcca agcagtaatc gaactatgct tatcacctaa gtcaaattca
    31441 gcaatgagtg ccattgatag tgcattgtcc gatattagaa acggtcatgt gggccaaatt
    31501 ccaaaccatt taaaagatgg acattatcaa ggtgctaaag atctaggccg atctattggt
    31561 tacaaatatc cacaccaata tgttaatggc tatgtttcac aacaatattt acctgataaa
    31621 ctaaaaaaca aaatttatta tgaaccaaaa acgacatcta aaagtgaaca acaactcaaa
    31681 gaaatatata acaacttact taaacaaagg ccgtaaaaat aagattaaat cgattacctt
    31741 tgatatttta gctataatca aatcaaaact gactagtaca cgaggcatat tctactatgc
    31801 ttgcgaacta gtcagtttta gttttatggt gtggttacac ttgccttacg ttgaaaatta
    31861 tccctttcaa aattaattat tgccctttgt ctttaatgcg acgaactgga atatctttta
    31921 atacgtcatt caccacataa cttgcacaaa ttaatccaac aacacttggc acaaaggcat
    31981 ttgaagaagg tggcatttgt ccttttcgat tgatagcatt tttatctcca actatatctt
    32041 ttacatcttc tcttatgaca attggacttt catctgaaaa tacaactgga atccctttac
    32101 gaattcctag tttttttaat ttttgacgaa taattttggc cattggatcg gtatgtgttt
    32161 tagagatatc tgaaattgta aaacgtgttg gatctgtttt atttgcagca cccatactgg
    32221 aaatcacttt aatccctcgg tcaagacact ctttcattaa gtgtactttg tacattattg
    32281 tatcacttgc atctacaaaa taatctatat cgtaattatc gaaaatttct tcatatgtct
    32341 cttctgtata aaacatatgt aaaggcgtga ctttacaatc tggattaatt aatttaatac
    32401 gttcttccat caaagaaact ttactttgtc ctaccgttgt agttaaagcg tgtaattgtc
    32461 tgtttacatt tgtaatatca acatcatctt tatctattaa tataatatga ccaatattcg
    32521 ttcttgctaa tgcttcagca gcaaatgaac caacacctcc aacgccaagt atgacaactg
    32581 tttgttgctt caataaatct aaaccttgtt gtccaatcgc tagttcattt cttgaaaatt
    32641 gatgtttcat tattttacct ctttcactga tttatacata agtacatagt aacttaaaat
    32701 tttatattta gcattattgc tttgattatt ttccaaaatt caacgaggaa acaattatta
    32761 aacgctacta aagccaacta attctttatt aaaaacttaa agaaacgcat aaaaatacgc
    32821 aagacaaagt cttgcgtatc gatagagtcc gtattgccgt agttataata gcttgatcat
    32881 tcggcctgtt atatacaggt gggtgccctg tttcttgttt tgtacgtcct tcatataagg
    32941 cgtgtacgct gcaagaaaac ccattgggct cccttgatca aagagtgtta ggcccaaatt
    33001 aaaaagcaaa cttacgaaca actcagatga ctatcttatg atgttatatt accacataat
    33061 taaaattaat gaaattataa caaaccaaag tttattgatt ttttaaaatt tagtgacgaa
    33121 ttcgcaaaga aagttcttct aattgtttat cagaaacttc accaggcgca ttcgttaata
    33181 aacatgtagc agatgctgtt ttagggaatg caattgtatc tctcaagttt gttctattag
    33241 tcaataacat gactaatcgg tctaatccta atgcaatacc gccatgaggt ggtgcaccat
    33301 atttaaatgc atctagtaag aagccgaact gttcctgtgc ttgttcttta gtaaatccaa
    33361 gaacttcgaa cattttttct tgtaactcac cgtcatgaat tctgattgaa ccgccaccta
    33421 attcataacc atttaatact atgtcataag catttgcctc agcttcttct ggcgcagtac
    33481 caagcttagc aatatcagct tcttttggag atgtaaatgg atgatgtgct gcaacgtaac
    33541 gtttcgcatc ttcatcatat tctaataatg gccaatctgt cacccataag aagtttaatt
    33601 ttgtttcatc gattaaacct aattctttag ctaatttgac acgtaatgca cctaaacttt
    33661 gtgcaacgac atttggtttg tctgcaacaa acattactaa gtcaccagct tcagcaccag
    33721 ttaatgtaag taatgtttca acattttctg tttcaaagaa acgtccaatt ggacctgtca
    33781 aaccatcttc cacaacttta acccacgcta atcctttagc accatagatg tttacaaatt
    33841 ctgttaaagc atccatatct ttacgagtat attgttcagc tgcaccttta gcgacaattg
    33901 ctttaatttc accatcattt tcaacagtat ctttaaatac tttaaagtcc atatcacgtc
    33961 ctaattgaga aacgtcaatt aattccattt caaaacgcgt atctggttta tcagaaccat
    34021 agcgacgcat cgcttcttta tatgtcatgc gtgggaaagc gccattaatt tcaacgcctt
    34081 taacttcttt aacaactttt ttaagcattt cttcacccat ttgcatcaca tcttcttgat
    34141 ctacaaaact catttcaata tcgacttgtg tgaattcagg ttgacgatct gcacgtaaat
    34201 cttcgtcacg gaagcatttt acgatttggt agtatttgtc aaatccacta atcatcaata
    34261 attgcttaaa taattgtggc gattgtggta atgcataaaa ttcaccatca tggacacgag
    34321 atggtactaa atagtcacgt gcaccctcag gtgttgactt cgttagtact ggtgtttcga
    34381 tgtcaaagaa cccttcatca tccaaatatt gacgaataga acgagtaatt tgatgtctca
    34441 ttttaaatgt ttgcgctaac tcttgacgac gtaaatctaa ataacggtat tttaatcgaa
    34501 tattttcatc aacgttaaca ttttcttcat ttatagaaaa tggtggtgtc tcagatttat
    34561 taatcacttt aatatttgta acttgtactt caacttggcc agttttaatt ttagggttaa
    34621 ctgtttcagg gtcacgcttc gtaactgtac cttgaacttc tacaacatat tcagaacgta
    34681 ctgtttcagc aattttcaat gcctcttctg aaaatgcagg attaaacacg acttgcacaa
    34741 ttccttctct atctcttaaa tcaacgaaaa tcaatccacc aaggtcacga cgattgttaa
    34801 cccatccttt taatgtaatt tcttgtccta aaaatgcttc agtaactaat ccacaataag
    34861 ttgttctctt actcatattt taacgccctc tctacttctt aaaatattcg actaatgcgt
    34921 ctaattcaat tgtttcagat tcaccagttg tcatattttt aacatcgatt ttattatttt
    34981 ctaattcttg atcaccaata acgattgtaa acttggcacc caaacggtct gcttgtttca
    35041 tttgtccttt aattttacgc tgtaagtagt ctttatctgc tttaatacca ttatgtctca
    35101 aatgatttaa taacttcaca gcatatcgat ctgcttgatc acccattgta acaatgaata
    35161 aatctaagtt ttcttcaata tctaattcga taccttcttc ttcaagtgca agcaataatc
    35221 gttctatact tagcgcaaaa ccaatacctg tttcgcttgg accatctagc aattctaata
    35281 aaccattata acggccacca ccacaaagcg ttgtaatggc accatcatag ttagggttat
    35341 ccatcattaa ttcaaatgct gtatgtgtat aataatccaa tccacggact aagttaggat
    35401 cttcaatata tgcaatacct aaatcatcta aataagcttt tacttgttca taatatgcct
    35461 tagattcttc atttaagaaa tcagtgattc taggtgcagt cttaatcgct tctttatcac
    35521 ggtctacttt acaatccaaa attcgcatcg gatttgtatg caaacgtgat tgacaatctg
    35581 aacaaaattc atgaattact ggttcaaagt gtttcactaa cgcttcgtta tattcttttc
    35641 gagacgccat atcccctaca ctattaataa caagctttaa atgttttaat ccaaatgatt
    35701 gataaatatg cataaccata gctaatactt ctgcatctac gctaggattt tcagcaccaa
    35761 tagcttctac gccaaattga ttaaattgac gatagcgccc tttttgctta cgttcatatc
    35821 taaacatcgg tccattgtaa taaagtttaa ttggttggtt tggattacct tgcattttat
    35881 gttcaatata tgaacgcaca actgcagctg ttccctcagg tcttaatgta atacttctat
    35941 cgcctttatc tttaaatgta tacatttctt tttgtacgac atcggttgaa tcaccaacac
    36001 ctcttgcaaa aagatctgta ctttcaaaaa ttggtgttct tatttcttta taattataaa
    36061 atgtcattaa ttcatctaat tgattttcaa tgtaacgcca tttctttgaa tcttcaggta
    36121 aaatatcctg cgtccctcta ggtattttaa tcattttgac atctccctcg gcaaactatt
    36181 ttatttaatt acctttttaa aactaaaact atatacgacc acttaacttt gcacgataaa
    36241 tcataatttt aaaagcaaaa gaaaaagccc ttgcacagcc tgaaactgca caagggacga
    36301 atatccgtgt tgccaccctc attggtttaa aaaacatgct taattgtaat catcattact
    36361 ttttataagc gtgcatttaa taaacccact caaaacgttt aacgtacgtt taacggcttt
    36421 acttttcata aagcacctct tcttgtgttc aatgtatata aactactcag cataatcttt
    36481 cagtctatgg attatattcc tacacaatct tagttagatt gctacctatt tcatttacca
    36541 tttcaagaat acaaacggtt ttagttataa aattcaatat caatgataac tactttcaca
    36601 tatttttgca agccctacgc agaaaaataa attttaagtc catcaacaat tgcttgttct
    36661 aaaatttgtc tatgtaattg atctttaatc atcgtttcat cagttgggtt actaatataa
    36721 cctaattcta ataaaacagc aggaactttt gtttgtctta acacttgata attttcttgt
    36781 cttgaaccgc gattagaaag tagacctttc ttctgaatcg tagcgtctaa cgtatctgct
    36841 aaagctcttt gattatcatg ataccaataa actgtcattc catttgcatt agatgattct
    36901 aatgcatcat tatgtatact caaataggca tcgcctttga tatcacgatt ttctagtgaa
    36961 acatatgtat cgtctgttct tgtcatctta acagttgcgc cttctttttc taaagtgcgc
    37021 tgcaattctt ttgctgtttt caacgtataa tctttttcta agcttttata tttagtattg
    37081 cttgaagcac cctggtcact acctccatga ccaggatcaa gcactattgt tttaccttgc
    37141 aaaggatttt tctcctttgt attatccgcg acaatatcta aatttgtgtg ccatccagct
    37201 atccaacctt tttcattact ggatgtatct tcaacttcaa tccatttacc tactttacca
    37261 atctttttaa aatggtcacc tttctcaact ttgtaaatga ctggatacgc agcgtttgga
    37321 cctgtacgta attcagcatt ttcagttatc gtaatgttcc cactatcttc actattgcta
    37381 ttcagcaata aaaataaaaa gatgataaat aagacaaagg caatcactac tattagagta
    37441 cgtttatttt taagaccctt tttagataac catgcctcta tttttttcat tgaattttgc
    37501 cgtcctgact ttcataaata atagtgactg gaccatcatt atttatgtta acattcatgt
    37561 gtgttccaaa ttcacctgtt ttcacagtaa gaccatacgc tcgtagcgca tcattaaaat
    37621 actcatatat ttttactgct tgatcaggat ttttagaatt tgagaaacct ggacggttac
    37681 ctttttttac atctgcatag agagtaaatt gtgaaactga tagtatttca ccattcattt
    37741 gttggatatt aaagtttaat ttattattgt catcttcaaa taatcttgca ttagcaattt
    37801 tctttgcaat tacatctgca tcttgctctg tagagtcctg accgataccg actaataaac
    37861 aatatccttt tttgatttga ttatttaatg tatcatttgt caccgatgct tctttaactc
    37921 tttgtacaac tactttcata ttttgcacct ctagttccaa actcttgtta ctgtataaac
    37981 atcaccaagt tgtttgatct tttctaccac acgataaaca tcattcacgt ttttcaccat
    38041 gacactaata tttattattg catttttatc aatatctgaa cgtcctgaaa ctttaattaa
    38101 attgccggct gtcgagctaa cagcttgtag gacttcattc aacaaaccat ttcggtcata
    38161 cgcagttacc tctaaatcaa cctggtattt ttgagttgcg tcttttgatt ttacccattc
    38221 aacattaatt agtcgttcag tttcgttctt aatatttggg caatcagtgc gatgtacttt
    38281 aataccgtga cctttggtga tataacctac aatatcatca cctggtatag gattacaaca
    38341 ttttgacaac ttgataagca cattttctaa accttctaca tagacaccac tatcagtaat
    38401 gatgttgtct ttaataggca atgatttcgt aacttcttgt gcttcattta aagcacgttg
    38461 tttatctaaa atacgttgtc tttcagttaa tttattaaca atctgtaagg atgtcacgcc
    38521 gccaaatcct acagctgcga ataaatcatc ttcatttgca aagttatatt tttcattaac
    38581 aacctgaata tttttctctg tcaaaatatc ttcgactcta aatccttgct cttttatttc
    38641 agcttcaacc atcattcggc ctttttcaat attagatgaa cgatcttgtt ttttgaagaa
    38701 acttttaatt ttacctttgg cactagacga tttaacaatt ttcaaccaat cacgacttgg
    38761 tccatatgaa tgtttactag tacgtatttc aacaatatcg cctgtttgta aaatatagtc
    38821 aattggtaca attttgccat tcaccttggc accaatcatc ttattaccga cttcactgtg
    38881 aatcgcataa gcaaaatcaa tcggcacagc accatatggc aactcaataa catcactcgc
    38941 tggggtaaat gcgtatactt tgtcactctg taagtcatat tttaaggttt ccataaattc
    39001 ttgagcgtca gacgatgtat gatccgcttc agctaattct tttaaccaat ttaacttatt
    39061 ttgataagtt tgatcttttt cacttacttt tttaccttct ttgtaagccc agtgtgctgc
    39121 aacaccatgt tcagcaattt cgtgcatatc aaacgttcgt atttggattt cgagcgggtc
    39181 tccatttgga cctactactg tagtatgcaa tgactgatac aaattttgtt taggcattgc
    39241 aatataatct ttaaaacgtc ctggcatcgg tttccataac gtatgcacca atccaagtat
    39301 cgcataacaa tcattaatag aattgacaat aacacgtatc gccaacaaat caaaaatttg
    39361 atcaaattgc tttttctgct tcatcatttt ccgataaata ctgtaaatat gtttaggtct
    39421 accatttata tcgccttcga tattcattcg gtccatttca gtacgtattc tatcaatagc
    39481 cgtttcgata tacgcttcac gttcactacg tttcttcttc attaaattga ctattctaaa
    39541 atattgtaca ttatcaatat aacgaagagc cgtatcttct agttcccatt tgattgtatt
    39601 aataccaaga cgatgtgcta aaggtgcata aatttctaat gtttctcgag aaattctaat
    39661 ttgtttttca cgcggcatgg ctttcaaggt acgcatatta tgtaatctgt ctgctaattt
    39721 caccaaaatt acgcgtacat ctttggcaat cgcaataaat aacttgcgat gattttcagc
    39781 ttgctgttct tcttttgagc ggtattttac ttttttaagc ttcgtcacac catcaacaat
    39841 tcgagcaact tcttcattga acatttcttt tacatcttca aatgtatacg gtgtatcttc
    39901 aattacatca tgcaaaaaac ctgcgacaat cgtcggtccg tctaatcgca tttctgttaa
    39961 aatacctgca acttgtatag gatgcataat gtatggtaat ccgttttttc ggaactgacc
    40021 tttatgtgct tcataagcaa tatgatagct ttttaaaaca tactcatatt catctgctga
    40081 caaatatgat tttgctttgt gaagaacttc gtctgcacta tatggatatt cgttgttcat
    40141 tatatgatac accccattca tatttattac ttcgccttaa acaatgattt aggtactctt
    40201 gttgaatagt atttgtccca caccaatcat acgtccgtcg acgataaata tttatcctgt
    40261 cgtcattaat cgtaatatta attttacttg agcgagttta atttgtatac tattcctact
    40321 tttaaaactt ttacaaaaat tcgacctaaa tctactgttt cattttttaa atattagttc
    40381 tatgatacta caatttatga aataaataaa cgatgttatt aaggtataat gctcaatcat
    40441 ctaacatttt cagtaaataa aaaatcctaa catctcatgt taagaaaact taaaca
//
LOCUS       DAHHRR010000010        36999 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.010, whole genome shotgun sequence.
ACCESSION   DAHHRR010000010 DAHHRR010000000
VERSION     DAHHRR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36999
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.010"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..419
                     /locus_tag="NBI42_000433"
     CDS             <1..419
                     /locus_tag="NBI42_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCD1776894.1"
                     /translation="KDLNSERENVEDRKDNAQSGFVYIISNIGSFGENVYKIGVTRRL
                     EPMDRINELSSASVPFEFDVHALIFSENAFELENKLHEYFKKYKVNKVNGRKEFFKVN
                     INEIKDKVLSEHNSTVQFIDEPKALQYRETLRLTSL"
     gene            675..903
                     /locus_tag="NBI42_000434"
                     /pseudo
     CDS             675..903
                     /locus_tag="NBI42_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121211.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1004..1240
                     /locus_tag="NBI42_000435"
     CDS             1004..1240
                     /locus_tag="NBI42_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776895.1"
                     /translation="MIRFDVNGSDHANPPNNERIPTPHIHIYTEEYNNGGIAIPLKDI
                     EDLELTDEIIESLDFFMKYTNIKHDNVIIEPRLL"
     gene            1261..2037
                     /locus_tag="NBI42_000436"
     CDS             1261..2037
                     /locus_tag="NBI42_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HCD1776896.1"
                     /translation="MKTIEERMNEYFNWLKQNYIFKELDSSTEITTPFKNHLNDFIRI
                     YADTLPNNEICLSDDGLTINELEMLGIDINTKTRTKLIQNILNQFNLKLVDKEITADV
                     KNESFAQSKHNLIQGILKIYDLTLTTKSNVTNIFYEEVFEFLYDREIRGLAQVSVSGE
                     SGLKYSIDYIVSETKSQPEKLVNFTNNLDFNKITNEAYIYRDIKPNRPSRNKLEPIML
                     IIVNDVDHPINERAQTVAEHENLEILKWSNKSKIIETLTS"
     gene            2435..2928
                     /locus_tag="NBI42_000437"
                     /pseudo
     CDS             2435..2928
                     /locus_tag="NBI42_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253430.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2998..4047
                     /locus_tag="NBI42_000438"
                     /pseudo
     CDS             2998..4047
                     /locus_tag="NBI42_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456358.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(4110..4198)
                     /locus_tag="NBI42_000439"
     tRNA            complement(4110..4198)
                     /locus_tag="NBI42_000439"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4161..4163),aa:Ser,seq:gga)
     gene            complement(4253..4324)
                     /locus_tag="NBI42_000440"
     tRNA            complement(4253..4324)
                     /locus_tag="NBI42_000440"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4289..4291),aa:Glu,seq:ttc)
     gene            complement(4326..4400)
                     /locus_tag="NBI42_000441"
     tRNA            complement(4326..4400)
                     /locus_tag="NBI42_000441"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4366..4368),aa:Asn,seq:gtt)
     gene            complement(4418..4491)
                     /locus_tag="NBI42_000442"
     tRNA            complement(4418..4491)
                     /locus_tag="NBI42_000442"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4457..4459),aa:Gly,seq:tcc)
     gene            complement(4507..4582)
                     /locus_tag="NBI42_000443"
     tRNA            complement(4507..4582)
                     /locus_tag="NBI42_000443"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4547..4549),aa:His,seq:gtg)
     gene            complement(4596..4668)
                     /locus_tag="NBI42_000444"
     tRNA            complement(4596..4668)
                     /locus_tag="NBI42_000444"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4633..4635),aa:Phe,seq:gaa)
     gene            complement(4685..4760)
                     /locus_tag="NBI42_000445"
     tRNA            complement(4685..4760)
                     /locus_tag="NBI42_000445"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4725..4727),aa:Asp,seq:gtc)
     gene            complement(4781..4854)
                     /locus_tag="NBI42_000446"
     tRNA            complement(4781..4854)
                     /locus_tag="NBI42_000446"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4818..4820),aa:Met,seq:cat)
     gene            complement(5351..5905)
                     /locus_tag="NBI42_000447"
     CDS             complement(5351..5905)
                     /locus_tag="NBI42_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HCD1776897.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(6226..7626)
                     /locus_tag="NBI42_000448"
     CDS             complement(6226..7626)
                     /locus_tag="NBI42_000448"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HCD1776898.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGLDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYSNQQEDVF
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDDKDIENTYDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDSVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(7650..8573)
                     /gene="hemH"
                     /locus_tag="NBI42_000449"
     CDS             complement(7650..8573)
                     /gene="hemH"
                     /locus_tag="NBI42_000449"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCD1776899.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEYIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(8631..9668)
                     /gene="hemE"
                     /locus_tag="NBI42_000450"
     CDS             complement(8631..9668)
                     /gene="hemE"
                     /locus_tag="NBI42_000450"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCD1776900.1"
                     /translation="MVHNKNNTILKMIKGEETTHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDISVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            complement(9658..9765)
                     /locus_tag="NBI42_000451"
                     /pseudo
     CDS             complement(9658..9765)
                     /locus_tag="NBI42_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9929..10432
                     /gene="traP"
                     /locus_tag="NBI42_000452"
     CDS             9929..10432
                     /gene="traP"
                     /locus_tag="NBI42_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HCD1776901.1"
                     /translation="MKKLYTSYGTYGFLNQIKINNPSHHLFQFSTADSSVIFEETEEN
                     TVLKSPSIYEVIKEIGAFNEDHFYCAIFIPSTEDHVYQLEKKLISVDDNFKNFGGFKS
                     YRLLRPVKGTTYKIYFGFADRQTYEDFKNSDAFKDHFSKEALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            complement(10556..11779)
                     /locus_tag="NBI42_000453"
     CDS             complement(10556..11779)
                     /locus_tag="NBI42_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1776902.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFALIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFIIFALTYYLLLAPKWYIVFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(11772..12512)
                     /locus_tag="NBI42_000454"
     CDS             complement(11772..12512)
                     /locus_tag="NBI42_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1776903.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            12646..13068
                     /locus_tag="NBI42_000455"
     CDS             12646..13068
                     /locus_tag="NBI42_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCD1776904.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            13210..13575
                     /locus_tag="NBI42_000456"
     CDS             13210..13575
                     /locus_tag="NBI42_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1776905.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            14308..14865
                     /locus_tag="NBI42_000457"
     CDS             14308..14865
                     /locus_tag="NBI42_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCD1776906.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYGQYIEEHSTGINILKKIKNPHHL"
     gene            15070..16032
                     /locus_tag="NBI42_000458"
     CDS             15070..16032
                     /locus_tag="NBI42_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HCD1776907.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKNPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSDVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
     gene            complement(16152..17093)
                     /gene="yhaM"
                     /locus_tag="NBI42_000459"
     CDS             complement(16152..17093)
                     /gene="yhaM"
                     /locus_tag="NBI42_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HCD1776908.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(17090..20026)
                     /locus_tag="NBI42_000460"
     CDS             complement(17090..20026)
                     /locus_tag="NBI42_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1776909.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLSILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVINELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            complement(20016..21212)
                     /locus_tag="NBI42_000461"
     CDS             complement(20016..21212)
                     /locus_tag="NBI42_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCD1776910.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            complement(21503..21610)
                     /locus_tag="NBI42_000462"
     CDS             complement(21503..21610)
                     /locus_tag="NBI42_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776911.1"
                     /translation="MFNVSVGSLAVLTITEIAFSTEIFEKNQRTLQIKF"
     gene            complement(21984..22328)
                     /locus_tag="NBI42_000463"
     CDS             complement(21984..22328)
                     /locus_tag="NBI42_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HCD1776912.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            complement(22397..23521)
                     /locus_tag="NBI42_000464"
     CDS             complement(22397..23521)
                     /locus_tag="NBI42_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCD1776913.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTNHQNQTIASLLPNQLVTFLDQH
                     VDNATDLLCDRARKYLSSVKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDRPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEQLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIVEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            complement(23700..24164)
                     /locus_tag="NBI42_000465"
     CDS             complement(23700..24164)
                     /locus_tag="NBI42_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1776914.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            complement(24519..25142)
                     /locus_tag="NBI42_000466"
     CDS             complement(24519..25142)
                     /locus_tag="NBI42_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1776915.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            complement(25164..26276)
                     /locus_tag="NBI42_000467"
     CDS             complement(25164..26276)
                     /locus_tag="NBI42_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HCD1776916.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            26439..27260
                     /locus_tag="NBI42_000468"
     CDS             26439..27260
                     /locus_tag="NBI42_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD1776917.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTVDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKETVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISISLDD"
     gene            complement(27724..29109)
                     /gene="fumC"
                     /locus_tag="NBI42_000469"
     CDS             complement(27724..29109)
                     /gene="fumC"
                     /locus_tag="NBI42_000469"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCD1776918.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAALANFDLGKLSKAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     TLKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKVGQEISGWRYMLDRCEIMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENMGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(29305..29700)
                     /locus_tag="NBI42_000470"
     CDS             complement(29305..29700)
                     /locus_tag="NBI42_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776919.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITGILYYTLDGFYASLTGVLGTLVVL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            complement(30477..30629)
                     /locus_tag="NBI42_000471"
     CDS             complement(30477..30629)
                     /locus_tag="NBI42_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776920.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
     gene            complement(30654..31253)
                     /locus_tag="NBI42_000472"
     CDS             complement(30654..31253)
                     /locus_tag="NBI42_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HCD1776921.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNIEKHAVDFSQINILDYDDKKSYFEALGIPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            complement(31412..31882)
                     /gene="trmL"
                     /locus_tag="NBI42_000473"
     CDS             complement(31412..31882)
                     /gene="trmL"
                     /locus_tag="NBI42_000473"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCD1776922.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFETTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQNFPGLN"
     gene            complement(31887..33014)
                     /gene="queG"
                     /locus_tag="NBI42_000474"
     CDS             complement(31887..33014)
                     /gene="queG"
                     /locus_tag="NBI42_000474"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCD1776923.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDTALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPMIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            complement(33165..33887)
                     /locus_tag="NBI42_000475"
     CDS             complement(33165..33887)
                     /locus_tag="NBI42_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD1776924.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            complement(33880..35337)
                     /locus_tag="NBI42_000476"
     CDS             complement(33880..35337)
                     /locus_tag="NBI42_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCD1776925.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLLVAAALYFVLTFVLTRIMNMIEGRMNASD"
     gene            complement(35596..36657)
                     /locus_tag="NBI42_000477"
     CDS             complement(35596..36657)
                     /locus_tag="NBI42_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCD1776926.1"
                     /translation="MSNAKNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMILQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
BASE COUNT        12330 a         6444 c         5232 g        12993 t
ORIGIN      
        1 tcaaagattt aaattctgaa agagaaaatg tagaggatag aaaagataat gctcaatcag
       61 ggtttgttta tataatatca aatatcggtt catttggaga aaatgtttat aagattggtg
      121 taacccgtag attagaacca atggatagaa ttaatgaatt aagtagcgca tctgttccct
      181 ttgaattcga tgtgcacgct ttaattttct ctgaaaatgc ttttgagctt gaaaacaaat
      241 tacacgaata ctttaaaaaa tataaagtta ataaagttaa cggaagaaaa gaattcttta
      301 aagttaatat taatgaaatt aaagataaag ttttatcaga acacaatagc acagttcaat
      361 tcattgatga accaaaagct ttacaataca gagaaacttt aagattaact tcactctaaa
      421 aatttgggta gcccgcctac ccttattatt ttttgccaac ttttatgagt atgtcatata
      481 tcaaaaaaaa caaccaaatt gtaaaaggtt ttatggtttt ggatatgaat gtaattgttg
      541 taaaaagtaa ttttttatta atcaatacat tgtaatatta agtgcaaccc aataaaaatt
      601 cataaatata cccacaattg gcactttatt gtaaggtttt tacaaccacg aaaacacatt
      661 taagtattgc caatatgagc gataactatc ttatactaac tgagcacgta tagagaaaat
      721 tattctttaa gacctattgc acgtaaatta aaacaatccg catctactat cttacgtgca
      781 attagcagaa ataatgtaaa tatattatat caagctgaaa cggctcaaaa gaattatgta
      841 actacaagaa aactttgtgg tcgtcctact ggatttactc cagaacttag caatatcatt
      901 aagtaaaatt taaaaatacc actactcttc tgaattaatc gtgattcgat tttttaaaac
      961 cgattttctt ttaaaaccaa gtatttatgg tttaaccact gatatgatta ggttcgatgt
     1021 taatggtagt gatcatgcaa atccaccaaa taatgaaaga atacctacgc cacatataca
     1081 catatatact gaagaatata ataatggtgg tattgcgatc ccattaaaag atattgaaga
     1141 tttagaatta acagatgaaa ttatagaatc attagacttc tttatgaagt atactaatat
     1201 aaaacacgat aatgttataa tagaaccaag attactgtaa aagaaatgga gggatactta
     1261 gtgaagacta tcgaagaaag aatgaacgaa tatttcaact ggttaaaaca aaattatatc
     1321 ttcaaagaat tagatagttc tacagaaatt actactccct ttaagaatca tttgaatgat
     1381 tttattagaa tatatgctga tacactacct aataatgaaa tttgtctatc agatgacgga
     1441 ttgacgatta atgaattaga aatgttgggt attgatataa acacaaaaac gagaactaaa
     1501 cttatacaga atattttaaa tcaatttaat ttaaaattag ttgataaaga aattacagcg
     1561 gatgttaaaa atgagagttt tgctcagtct aaacataatt taattcaagg catattgaaa
     1621 atttatgacc ttactttaac tactaaatct aacgttacaa acatattcta tgaagaagta
     1681 tttgaattct tatatgaccg agaaataaga ggtttagcac aagtttctgt ttctggagaa
     1741 tctggtttaa aatattctat tgattatatt gtttcagaaa caaaatcaca accagaaaaa
     1801 ctagttaact ttacaaataa cttagatttt aacaaaataa ctaatgaagc ttatatttac
     1861 cgtgatatca aaccaaacag accatccaga aacaaattag aacctatcat gttaataatt
     1921 gtaaatgatg tagatcatcc gataaatgaa agagcacaaa ctgtagcaga acatgagaac
     1981 cttgagattt taaaatggtc taataaatca aaaatcattg aaactttaac ctcataaatt
     2041 agcagtagtc ccttattcat attctttaat tttcaaacca tatcgtgcaa atagaaaagc
     2101 agataacttt tttatttgca cgttttattt tagaacaaaa aagaacaagg tcaacgagtc
     2161 agaaaaactt tttcttgata cgtatttgac acttgctctt ttaagaatac taacatttca
     2221 ttgtatttct gttaaattaa cggagaatga atgatatctt tatactaata atcttaactt
     2281 tttaacatgt aaaattaata cattgtacta aagtttcaat tattttataa ttatataaat
     2341 gaatcgtata cacttagtta gaaaaatcta agaatttaga tattgtttaa ttataaaact
     2401 atcgattcac atataaataa aaggagataa cagtatgagg agattattca gtctattact
     2461 agctagtaca atgattttag tcgcatgtgg gaatgctagc aatgaaaata aggaattgga
     2521 agacgaaaaa aatcagaagt aaaaaaagaa gctaagataa ataataataa accaaagaac
     2581 gaaaagaaga atcaagatat aaacaaaaac aataatgagc aagttcaaaa tgcaaataat
     2641 aatcaaccag tcttaaataa taatagtaat cataacaatc aacaacaaag caataaaaac
     2701 aattacacag ttatagaaaa cgggaataca gttacagaaa tttataatgg acaaacattt
     2761 acaactacaa ataaccctat tagagaagga tatgtagagg gagaaacaga cccgatatat
     2821 aaaagagagt ataagcattt ttatacacct gaagaagctc aaagagcaca agaaaatagc
     2881 gaccaaattt taagagaaat gggtctcgaa ccagaaaggt atgaataaag tcaaactaaa
     2941 taatttcggg tagcccacct acccttatta tttttttgcc aattttgagg aggagaaata
     3001 aagtggcatc atttattaat acaaaaagaa gaataaacgt caacaccttg gcaatacgat
     3061 gtaaaacatc catctttcaa atcaaggagg aaaagaaagt caggattcaa aactaatgca
     3121 gaggcaacca acgccgcaca acaaatgatt aaagatttaa aggaaggtaa taaaattgaa
     3181 gataacaaaa agtttacaga atactacagt gactggataa atttaaagaa taaaaaacag
     3241 ttatccagca aacagtttta ttggtacgaa gtttcaatta agttatttag cgaggttttc
     3301 ggattaaaca tgctagtaaa aaatataact cgaagtgaat atcagaggtt tttaaataaa
     3361 tacgcacaag gtcataccga tgaaactgtg agaaaggttc acggttgttt agcaagaagc
     3421 atcagagacg cattatatga tggctattta aaaaaagacc ctacctatga cgttagtatc
     3481 aaaggcactg aaaagctaaa gatgagaagt ttaagtatat aacgattaaa gactacttaa
     3541 acttactaga gtactttaaa aaaagatgaa gaaagttata tttttctgta tctaataggt
     3601 attactggtg caagatatag cgatgttatc aatatgactt tcaacgattt aaacaaagca
     3661 aatgatataa ttcatttatc aggaacaaaa acaaagaatt cgaaacgaga cgttgaagtt
     3721 aatccaagag atattatgcg cataaattca aagttggtta gattgccacg tagaataaac
     3781 ggcaaattat ttttggttag tcatacatca gttaccaaat catttaaaaa agcaacagaa
     3841 gtaataggat taaataatga taacatcact ccctattcta ttagacacac tcacacatct
     3901 tacttattat caaaaggcat accgattgaa tatattagta aacgattagg tcactctaca
     3961 atatcacaaa cgttaaacac ctaatctcac ttacttgaag aacataaaaa agagcaaggt
     4021 caacgcgtca gaaaattatt ttcttgacac gtatttgaca cttgctagaa taaaaaactg
     4081 ttaaatcaag gttttaaagc ttatttttac ggaaggtaag ggattcgaac ccttgagacg
     4141 cttgtgacgc ctacacactt tccaggcgtg ctccttcggc caactcggac aaccttcctc
     4201 aacaataaat atgtaataaa aaaacagaag cgatatttca cttctgtttg tatgactcct
     4261 acgggactcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaagg
     4321 agccatggct ccacaggtag gactcgaacc tacgaccgat cggttaacag ccgatagctc
     4381 taccactgag ctactgtgga ataataatag aaaataatgg agcgggtgat gggaatcgaa
     4441 cccacaacat cagcttggaa ggctgaggtt ttgccattaa actacacccg ctaatgtaaa
     4501 cgattatggg gcggctgaag gggatcgaac cctcgaatgt cggaaccaca atccgatgtg
     4561 ttaaccactt caccacaacc gccatgaatt aagaatggtt caggacagag tcgaactgcc
     4621 gacacatgga gcttcaatcc attgctctac caactgagct actgaaccgt aatggtaata
     4681 ataatggcgg tctcgacggg aatcgaaccc gcgatctcct gcgtgacagg caggcgtgtt
     4741 aaccgctaca ctacgagacc tattaaatta aaactatgta ttgcgggagg cggatttgaa
     4801 ccaccgacct tcgggttatg agcccgacga gctaccgaac tgctccatcc cgcgctaata
     4861 ttattttgaa ttacctaatt aatataccat aatcaaaaac ctaaagtcaa gaactttttg
     4921 aatttaattt aaatgttatc tcttaaataa ttacttaaat atcgtagcaa catgttctct
     4981 gttgaacaca aatattagta tattcatttt tgtagtaaac gtcaacgaca ttttcaaagt
     5041 tttttgtgta aaaaaacgct tcttattccc ttttatcata taagtgtcta atagttgtca
     5101 taaatagtgt taaagcattt taaaaggtat aggaatcata aagtttataa cgcctatacc
     5161 ttctgaaaaa gaaattataa aagcttgtta caccgcatat tcattcagtc agcaactacc
     5221 aatataacgt tgtagcccta agacattgtt tgatgcctca gtctcttcat tttgttaaaa
     5281 acttaattac cacaattttt ttaaaatcag cagctaccta ctgacacaac ataacacaac
     5341 aatacattac ctatcgtgtc atgtaatctt gcatccgatc ttgcaacgct gtaaatgttt
     5401 cgaagccatc ctcttctaag aagtgccctc catcttccac gattcgcaag ttcccctcta
     5461 atgcattcat taaacgctgg gtttctttat atgaaacgta tttgtcattt ttagaactca
     5521 atccgtaaaa attgtcaact ttctttttaa tattatcgta atcaatggtt acattactta
     5581 aatcaatatc taaatctata ttttctgcat cttctttaaa gcccgctata ctgaaaaagc
     5641 cttcaatcgg ctgatcaatc atttcaatat attttaaagc tgtgattgaa cctaaaccat
     5701 gtgttacaaa atatgtatcc tttttgcgta cattaatttg tttcgtcata gcttcaatcc
     5761 actgatccac tgtcttcgct tcaggggatt caaaattaaa taatgttacg tcatatcctt
     5821 ctaaagttaa gttatgctcc aaccactgat accaatgatt tctactattt ccatgcatag
     5881 aatgtacaat gattacatct gtcatctcat tctctccttt caacttacta tttcttttct
     5941 attttaaaaa aatgaatgat tacctataat tgtaaaataa aaacacctta attagaaatg
     6001 ttatttcgca aagtgacatt tctaattaaa gtgcattgtc atcatttcaa tatcattcaa
     6061 taatagctaa acctttgtct ctgattcagt ttcatgaaat aattcccgct gaaagtatca
     6121 tgatttgcac attacttcca ccattatata acttaaaaat gactatattt catcaaacat
     6181 tatctaatgg catcgcacca acaccaacac catccagcaa ttaacttaca actctgcgat
     6241 tacttcttca gcagcaactt taccttgtgt aatacaatca ggtagtccaa ccgcttcaaa
     6301 agatgcacca gttactctaa gtcgtggata tgtttgttta atatgtgctt gaatctgtct
     6361 aatttgttga atgtgaccaa catggtactg tggcatactt ttcggcaaac gattgacaat
     6421 tgtaaattca ggatcacctt taaatgtcat catttgactt aaatctctac gtacaatcga
     6481 tactaattca ttatctgtat gatcatcaac cacactatca cctggtttac ctacataagc
     6541 acgaatcaaa accttacctt ctggtgtagt aaatggccac tttttcgatg tccaagtaca
     6601 tgcggtaatg tcagtatcac tcgttctcgc aatcacaaag ccagtaccat cataagtatt
     6661 ttcaatgtct ttatcatcaa atgccaatac aacagttgca acagtcgtac tatccatcgt
     6721 tttaaagtaa tcaaatgctg gatcttgccc gaaccaattc aaaaagactt gatgcggtgt
     6781 tgtcactaat accccatcga atacatcttc ttgttgatta ctgtaaacaa ttttatattg
     6841 cttttgagat gtaataatat catccactga cgtattgtag cgtattgtca cacctttatt
     6901 tttcacatct tgttctaatg cttcaataaa tgagcttaaa ccatgcttaa attgtttgaa
     6961 ttgtcctttt ggtgcgccag gatataattg tctttgtttc agacgcttat ttttctcatc
     7021 cttcatacct tttatcagac ttccgaatgc ctcttctttt tctttaaaat taggaaacgt
     7081 actcatcaaa cttaatttat caatatcggt accataaata ccacccatta aaggctcaat
     7141 taaattctca agtacctcat tacctaatct tgctctgaaa aatgcaccaa cagaaatgtc
     7201 accatcctgc atttgagtag gttttttaag taaatctaac cctgctctta actttccaag
     7261 tggtgaaatt aatttagttg tcacaaacgg tttgatatct gtcggaattc ccataattga
     7321 tccacctgga ataggataca atttgttttt cgcaaaaata taagattgtc cagtcgtatt
     7381 tgtaacaata tcttgttcta atccaatatc tttcgctaat tctgtcatga tcgtttttct
     7441 acctaaataa gattcaggcc ctagttcaat catataacca tctttacgat acgattgaat
     7501 ctttcccccc ggacgattcg atgcttcaaa gatggttaca tcaatattag gatcttgctg
     7561 ttttaaaaaa tatgcacttg ataaacctgt tatccccgct cctataatag ccactgattt
     7621 agtcacaacg tttcacgctt ctttcgtcat taaaatatag acttgatttc atcaacaatt
     7681 gcaccgataa ataatggatg tgtattcggc atttttggac gataataatt cgcaccaata
     7741 tcatcgcaaa caactttaca ttcataatca ttgtcataaa gcacctctaa atgctcacat
     7801 acaaaaccta ctggcgtata tataaagttt ttatactgat gtttttcata taaatcacgt
     7861 gttaaatctt gtacatctgg ccctaaccaa ggtgtacctg tattaccttc agattgccaa
     7921 ccaatcgcga tatattcaat attagattgt tctttaatta aaagcgcagt atgttctagt
     7981 tcttgtggat atggatcatt attcttttcg attaaacctt ttggcaaact atgtgccgaa
     8041 acaactaata ccgtgtcttt atgttcctct tccggtattt gagctaatgt ttcgttgact
     8101 ttattcgtcc aatattcaat aaatttaggt tgttcataat aatgtttcac atgtgtaagt
     8161 tgaataccat attttgcagc ttcttcatca gcacgtttgt catatgatcc tactgaaaat
     8221 gaagaataat gtggtgctag tactacagtg attgcttcag taatgccatc attgtgcatt
     8281 tgttcaaccg catcttcgat aaatggtgaa atgtgtttta atcctaagta tagtttaaat
     8341 tcaacatctg catatgcttt atttaatgct gaaactagcg catcagcctg gtcatctgtt
     8401 gtacctgcta atggtgataa accacctata aattcatatc tatctttcaa atcttgaagt
     8461 tcttcttcag atggacgttt accatgtcta atatctgtat aatatggctc tatgtcactt
     8521 tctttataag gtgtgccata agccataact aataatccca tttttttagt cattgataat
     8581 accttccttt aaatgaatta tctttcatgt gcttcaatgt aatactatga ttatctttgt
     8641 gtatatgtgt gtacgaattc gcttacttta cgtaacgtct ctggttgcac ttctgggaaa
     8701 acaccatgtc ctaaattaaa gatgtgttta ccgttctcca taccttgatc taatattggt
     8761 ttcaatctct cttcaatgac attccatggt gctaataaaa ttgatggatc taaattccct
     8821 tgtaatgttt tagtaacacc taattgttga gcctgattaa tagacgttct ccaatctagg
     8881 cctaatacat caatcggcaa atcattccat tcattgatta aatgactggc acctacaccg
     8941 aataaaatta ccggcacatc atgtttttct ttaacctcac tgattaatcg aatcatatgt
     9001 ggtttaatgt aacgtctgta atcctcgaca tttaatgcac ctacccatga atcgaaaatt
     9061 tgaatcaatt cggcacctgc ttcgacttga gctgttacat atttaacaga tatatcaact
     9121 aaatgattca ttaaagcaaa ccatgttgct tcatctctat acatcatcgc ttttgtaaaa
     9181 ttataatttt tcgatggtcc gccttcaatc atatatgacg ctaatgtaaa tggtgcccca
     9241 gtaaatccta ttagcggtac atttaacttt tcttctgtta aaagtttaat tgtatctaat
     9301 acatatggta catctcgttc ggggtctatt tgagaaagtt tctcaacatc ttgaattgtt
     9361 ttgataggat tatgaatcac tggaccaata cccgatttaa tttctacatc gacaccaatt
     9421 ggctttaatg gtgtcataat atctttgtat aaaattgctg catctgtatg ataattatca
     9481 actggcaaat gtgtaacata agcgcacaac tccggctgat gtgtaatatc gaatagtgaa
     9541 tatttttctt tcaattttcg atattctggt tgcgaacggc cagcttgtcg cataaaccaa
     9601 acaggtgtat gtgtcgtttc ttcaccttta atcattttca aaattgtatt gtttttatta
     9661 tgcaccataa aggcctccta aattaaaatc attcttatct atattatcat atcgctcatt
     9721 cgttcgtatt ttcaataaat aaatgtcata aaactgacat taaattacac atcatttgtt
     9781 tgtgtattta atttttaaat cttgatggat tatctccaca tctttttgtc ttgcatgatt
     9841 tattatagta actttaaccg tatattaata gtggtaatga ctagtttatc gtcgtataat
     9901 aaataaaaac ataaggggga cctttcatat gaagaaatta tatacatctt atggcacata
     9961 tggattttta aatcagataa aaatcaataa tccatctcat catttattcc aattttcaac
    10021 tgctgattca tcggtgattt ttgaagagac tgaagaaaat actgtactga aatcaccttc
    10081 aatatatgaa gttattaaag aaataggcgc attcaatgaa gatcattttt attgcgcaat
    10141 ttttattccg tcaactgaag accatgttta tcaacttgaa aagaaattaa tcagtgttga
    10201 tgacaatttc aaaaactttg gtggctttaa aagttatcga ttgctgagac ctgttaaagg
    10261 tacaacatac aaaatttact ttggctttgc tgatcgacaa acttatgaag actttaaaaa
    10321 ttctgatgct tttaaagatc atttttcaaa agaagcatta agtcattact ttggttcaag
    10381 cggacaacat tcaagttatt ttgaaagata tttataccca ataaaagaat agattcgaag
    10441 aaaacttcca ttggttatgc tgttaacgta tagatgctaa tgtaatctga acgccgtatc
    10501 atttggcgtt caacattaca tgcaccttta caacatacat gccaatggaa gttttttagt
    10561 ctcgtaataa tgtttcctga tattttaact ttttaataat acttctaatc gttaataaac
    10621 ctacaatata gaaaatgagt acaacataga ataacgtcat atgttttata ataaatgtca
    10681 ctgcaaacac agtacaaata acaaacatta atctatataa aaactgttca taccctttga
    10741 ttaccttctc ttcaggtaca ggccacactt gaggccataa gccatatgct tgttgagaat
    10801 aaaattgtgc catttgtaac aatataatat atacaaataa acacccgata attgctgtca
    10861 ctaatggata tgataaccaa accataagta aaactgcaat aattactaat ctaaagataa
    10921 tattaaacgc gtctctccct cttataaagc ttctaataaa taagaataaa tacatcgcat
    10981 tagagttaaa tttactaccc tttggaactg gtaaaagtat atctaaataa cttcttctga
    11041 ctgcagattc tttcaaatgt tttacatcgg tgaacatatt aacaaattta taataattca
    11101 tatgatgtcg atgttcgatt gcaatcattt tctcccaagg atacaaaaag ccaggtttat
    11161 attttttaac taaaaattct attaacacag gcaaagcaac catcacaaat acgatgtacc
    11221 attttggagc taatagtaag taatatgtta gagcaaagat gatgaatgat attaaattaa
    11281 cttgccatgt tttaagtccc gattgatacc attgccatct taagcgtaaa ccaacatatg
    11341 gaaaaattaa tgcactgact ccaaaacaaa tataaaatgc cacattatgt tgattaatat
    11401 tgtaaaacaa tgggaacatt acaattacaa taatgagttg gattaatatg cgcgcaaagt
    11461 aactatataa aatcgcatgg cgcataaatt gagacatgtg tttttcaaat ggtaataaaa
    11521 agattttatc cgcttctttt aacagtggtc gcattggaaa aatggatgtc aacgcaacaa
    11581 tcactgctgc tattaatgca aaattgatat tcgttggaat atgttttaac cattcaccat
    11641 atccaaaaat aaatgcaccc agcaaaataa gtaaaaagac catgaaatga ccattaaata
    11701 taaacttatt ataataattt ttctctttac gaagggcatg taatctttta ttaaataatg
    11761 tggtcgcttg gttacgcatg tacatctcca ccttgcgtca catgaatata tatatcgtct
    11821 aatgtttgat tatgtaagcc agtttgttgt ctcaatgctt ctaaatctcc aaatgcaacg
    11881 acttcacctt cgtctagtat gataaaacga tcacaataac gttcagctgt tgctaaaata
    11941 tgtgtactca ttagaacggt tctaccttcg tttttctttt caaccattaa atctaacatg
    12001 gattgaattc ctaatggatc taggccaagg aatggttcgt ctataatata caattcggga
    12061 ttaacgataa acgcacaaat aatcatgact ttttgtttca tccccttaga aaaatgactc
    12121 ggaaaaactt tcaattcatt ttctaaacgg aatgttttta ataatggcat tgctcgattc
    12181 atcgcttcat cacgatcaat atcatatgcc attgctgtca tctcaatgtg ttcctctaat
    12241 gtgagttctt cataaataac cggtgattcc ggaatataag ataactttct tctataagcc
    12301 tctatgtcat cattaatgtt gatatctgaa attgatagag atccttccat aggtgtaagc
    12361 aatcctagca tatgtttaat cgttgtactc ttaccagcac catttaggcc aataagtcca
    12421 acaatttcgc ctttgtttaa ttcaaaattt atatctttaa ttacagggcg ttttccatat
    12481 ccacctgtaa gctgttctac tttaactgtc ataaggcacc tccatgactt atattgtacc
    12541 aaaaattata aaatgctcat attaaataca catgtcctaa tatcgaattt ttagcgacaa
    12601 tgttataatt aatggtaata caagttgaaa aggagtgtag tcatcatgtc agaaacaatt
    12661 ttcggcaaaa ttttaactgg agaaattcct agctttaaag tatatgaaga cgattatgtc
    12721 tatgcctttt tagatatatc acaagttact aaaggacata cgttattaat tcctaaaaaa
    12781 gcttctgcta atatctttga aactgatgaa gaaacaatga aacatatcgg tgcagcatta
    12841 cctaaagtag caaatgctat taagcgtgca tttaatcctg atggtttaaa cattattcaa
    12901 aataatggtg agtttgcaga tcaatctgta tttcatattc atttccactt aattcctcga
    12961 tatgaaaatg atattgatgg atttggttat aagtgggaaa cacatgaaga tattttagat
    13021 aacgatgcaa aacaacaaat tgctgaacaa attcaagcac aattttaaat gtatgcttaa
    13081 tctaagctcg aacgggtata atatgattaa tattataaca attgcgtttg aagtgataac
    13141 atcaaggtta gtaattttaa acaaaatgag ttatcaagat aacagatgtt aaaagtgagg
    13201 agaatataaa tgaaagcatc acgcattcta ttcggtatcg gtgttggcgt agcagctggt
    13261 tttgtagttg cacttcaagg acgagacgac aaaagtgtca agaacaacac gatcgatcgt
    13321 actgccccta ctggttcaaa atcagaacta caacgtgaat ttgaaacgat taaacaaagt
    13381 tttaatgaca ttttaaatta tggtgttcaa attaaaaacg aaagtgcgga atttggtagt
    13441 tcaattggtg gtgaaattaa gtcattactt ggaaacttca aatctgacat taatcctaat
    13501 attgaacgtt tacagtcaca catcgaaaat ttacaaaatc gtggcgagga cattggaaac
    13561 gaaatttcta agtagcacgt tacgttctcg atcacaacta tttttattag taacagcata
    13621 tttatttttt aaaattaaat gccaaataaa cgagatgaca ttagaaatta gatatttctt
    13681 gtcatctctt ttttaaaact caaatgaact tatgtttaca aattatagga agacattgtt
    13741 tgtagtgatt tttacttaaa tcatatttat gaattgcctg aaaacattac ttaggattca
    13801 ccgtgttatg cttgcacttt gattacgctt tacttaaatc attatcgaca aacaacatac
    13861 ttatattttc attcagccga accttatata cacattacat ataccttact tgcacaaatt
    13921 attaatctgg tgtttattat cattacatat cactatattt ttagcatttg tatgacttag
    13981 ttggtcaaaa gatgcttttg catatgccct gccataccca ttttttatat tattcaagtt
    14041 ttcaaatact catgtcacac gcgccttttt cataagtgat gctttattag caagaatatg
    14101 cgttcgcaga aatttgttct gcattctact tctacgctag tcaatcagac aattttacca
    14161 atccccactt tcgcgtttca aatcaaacaa tacgtcgccc ctttcttcat atataacaat
    14221 tcttctaaca tgatatgtta ctattgaatt actgaacctg agttagttat aatctaactt
    14281 atattgaaaa gagatgaggc gtaagatatg tttttatgta aaagacaaat tgatatcaat
    14341 gcacgatttg gtttgcctag aattgcattt atgagtgcag ttgcaaccat cattatgttt
    14401 ttagttagtt atgaagtaat gtatttttta tctaatacgc cattatcaga tagacatttt
    14461 ctcatctttt tattacttgt atttatgacg tatccattac ataaaagtat acatttatta
    14521 tttttcttac catatagaaa atcgtttaaa gttcataagt tgactaaaag aaaatggctt
    14581 atattctata atacctacgt caatcaacct gtacacaaat tttatttttg cattaactta
    14641 atattgccgt taattatctt atctgcaatg ttcgtttatc taacaatttc attcccgcaa
    14701 tatggacatt attttatgtt cttattggca ttgaatttcg gtatttccat tacagattta
    14761 ttatatttaa aaataattat attttctaat tatggacaat atatagaaga acatagtaca
    14821 ggtattaata ttttgaaaaa aattaaaaat ccacatcatt tataacaaaa taattatagc
    14881 aaggtgttat tatttgtttt taggctatgt aatagcttac aatcaaatgt atatagacct
    14941 tgttttttta ttttcatcaa tttctacccc taaacctaat actctagtct gatgtcatgg
    15001 gttattgatt ggtgataata taaaactatg ttatattcac gatgattaac ttacaaagga
    15061 gtttcaacta tgaagatgat aaacaaatta atcgttccgg taacagctag tgctttatta
    15121 ttaggcgctt gtggcgctag tgccacagac tcaaaagaaa atacattaat ttcttctaaa
    15181 gctggagatg taacagttgc agatacaatg aaaaaaatcg gtaaagatca aattgcaaat
    15241 gcatcattta ctgaaatgtt aaataaaatt ttagctgata aatataaaaa taaagttaat
    15301 gataagaaga ttgacgaaca aattgaaaaa atgcaaaagc aatacggcgg taaagataaa
    15361 tttgaaaagg cccttcaaca gcaaggttta acagccgata aatataaaga aaatttacgt
    15421 actgctgctt atcataaaga attactatca gataaaatta aaatctctga ttctgaaatt
    15481 aaagaagaca gcaagaaagc ttcacacatt ttaattaaag ttaaatctaa gaaaagcgac
    15541 aaagaaggct tagatgataa agaagcgaaa caaaaagctg aagaaattca aaaagaagtt
    15601 tcaaaaaatc caagtaaatt tggtgaaatc gctaaaaaag aatcaatgga tactggttca
    15661 gctaaaaaag atggcgaatt aggttatgtt cttaaaggac aaactgataa agattttgaa
    15721 aaagcactat tcaaacttaa agatggtgaa gtatcagacg ttgttaaatc aagtttcgga
    15781 tatcatatta ttaaagctga taaaccaaca gactttaaca gtgaaaaaca aagcctgaaa
    15841 gaaaaattag tcgatcagaa agtacaaaaa aatccaaaat tattaactga tgcatacaaa
    15901 gatctattaa aagaatacga tgttgacttt aaagatcgtg atattaaatc agttgtcgaa
    15961 gataaaatct taaaccctga aaaacttaaa caaggtggcg cacaaggcgg acaatccggc
    16021 atgagccaat aacacaaaac cgagcgaccg tggttcaaaa atcataccac ggccgctcgg
    16081 tttttcgcat taaaaatcgg acagatgagc tcatgtttca gtatactcat ctgtccgata
    16141 tcttttaatt cttaatcgag tgattcagga ttgtagaatc tacgattttc aagaccaaat
    16201 attttatctg taaactgacc cttgtcagtt tttttatatg ccttttcaaa catattcatt
    16261 ctagcatcga tattatcgat atagcataaa atttctgctt cttttaagta tggcagtttt
    16321 ggcgaaccat actctaactt accatgatga gataaaatca tatgtcttaa caacatgatt
    16381 tcttctcctt caatgttcaa ttcacgagct gcttcaacta cttcatcact cgcaatcgag
    16441 atgtgtccta ataagttacc ttcgactgta tacgacgtcg caacaggacc actcaattct
    16501 ctgactttac caatatcatg caaaataata ccactatata acaaactttt gtttaacaat
    16561 ggataaatgt cacaaattga ttttgcaata cgtaacatcg ttaatacatg atagcttaag
    16621 ccactcgcaa agttatgatg atgagaacta gcagctggat atgtgtaaaa tcgttcttga
    16681 tattttttca ataaatgacg tgtgatacgt tgtaaattag cattttcaat atctagcaaa
    16741 taatgagaaa tctcttcttg tatttctgcc ggtgataaag gtgcaccatc tacaaattgt
    16801 tctgttttta attgatcttc agttgtcgct agtctaattt gattgacttt catctgttta
    16861 tttccgcgat agtttatgat gtcaccttta acatgtacaa tttcttcagg cttgattgtt
    16921 gccatatcat tttttgtagc cgtccaaaat ttcgcttcaa tttcaccact tttatcttgc
    16981 aaatgtaatg tcatataatc tttaccttgt gctgttacac cctgtgtagc tttatgcact
    17041 aagaaaaagt gatcaactga atctccggga tttagattct ctatatttct catcgtttcc
    17101 cgccttcctc tattttgttt aatgtaatca cttcttttga tggaacaata ttatctttta
    17161 cacatgtaaa gtatagtact tgatagtgtt ctgataatga tcgtaaataa ttcaacattt
    17221 tttcagtacg ttttttatca aaatgaacaa atgcatcatc aacaattaat gggaacggat
    17281 aatatggtcg tagtacctta attaaactga tacgtaaggc tacataaagt aattcttttg
    17341 tagattgact tagttcaaca ggatcatata attgaccatt aacatgttta accgtaattg
    17401 aatcttcatt atagttaatc atcgtatatc tgccatctgt taaatgcttc aatatttcta
    17461 ccgcttcatt aataacttga ggcaaacgtt tatctttaat ttgtttaatg tgttcatcaa
    17521 ctaaactttg taaataactt aaacttgccc aatcttttgc gatatcatta agttgatttt
    17581 taagactgtg atattcatgt cttaaattag caagcgttgt atcagtttcc atgtgattga
    17641 tttgtgcact taaatcactg acttgtgctt gcatttcaag atattgctca ttgtattcgt
    17701 caacttgagt agccaataaa tgatcttctt cttcaagttg tgctgttgtt ttttcactta
    17761 aactagaact taattcataa gaatagtttt ggttctcaag atatttagtt aaatcattaa
    17821 aacgactcaa attactagta taagtttggt aatcttcatg atgttgataa aaatcttctt
    17881 cagtaccaac attgataaaa tcgaatagtg ctgtaatttc tttattattt tcttctaatt
    17941 gagcatttaa atgatttaat tcatttataa caagtttggt attttcagca ttaatacgcc
    18001 atttttcatt cgtgtcttca gctgatttca accattgttg cacatcgtgg aataaagata
    18061 atttgttgaa ataaacaaat tgtgattttg taacagcttc agcatgattg tagaatgtat
    18121 ctaattcttg aactaattgc tggcgttgtt gatttaaatc actgatatgt tgatctaatg
    18181 ctttaatatt cgccattgta gaaatactat caacaattaa atcatttgaa attttagatg
    18241 ataagtataa ttcatcctta acgttctcaa ctgtcgattg caattcatca tgacgccctt
    18301 tcgcatcatt taaacgacct tcaatatact gacgtttctc ttctaaaata tctttatttt
    18361 tcaaagcttg ttgccagtga tcacgaatgc gatattgctc atcaagatca aaatctaagt
    18421 catagttttc atctaaaatg gatagttgtg ctttaatttc ttcgatttca tctgtgatgg
    18481 cctcgctata atctacttct tttgatttag acatgatgat accgataaca aatactaaag
    18541 ttaatactgc gaaaataata ccaaacaaca tgttgtttga aataaatgag aaggcagtta
    18601 aaccaatacc tactaatgtt aaaagaataa acgttgttcg taacaatttt tgacgttttt
    18661 gtttttcttg ttgctcaatt tcgaaacgtt ctttcaattt gctatacaag ttctcttttt
    18721 cgtttaattc aatgacttgt tgtgagtatt cttttttctt ttcaaaagtc tcttcaggaa
    18781 ctattttttc ttcaacagaa tctagttcgc tatgaaccgc attatcttcg attttatttt
    18841 cttctaaact acgttctaat tgcttaatgt atgccgcttg ttcttgttta ttcttgattt
    18901 gctcactgac ataacttttc attgcctcag agctatctac gtcatgatgc gtttcagacc
    18961 aaccaatatt tgcttgcaat tcatctttat cacgttgttt attcgcaata tccttttcga
    19021 ttgcagtaag ttcaaattct ttatttttaa tttcattttc ttgttgattc aaactaatga
    19081 aggcgtcaat atcagattgt ttaactggct ctaattgagt cgcttcttct ttaagttgag
    19141 ctaaacgctc atttcttaaa ccaatatctc tttctaacga ttgcttatgc gctcgtgctt
    19201 tttcgtaacg atccacacct ttttctggga atgttattgg ctcaatattt aactgttgtt
    19261 ctagagactt ccattcttgt gaatgatcat gtaaagcaac ctctttttgt ttttcttcat
    19321 gcatttttga taattgattt aaattatgct ttaaattctc taatcgacgt gatgatttat
    19381 ctcgatcatc tactaagcga tgatatgttt ctagtttagc ttcttcttca cgaatttgac
    19441 tttctagttg ttttaattgc tcaatttgtt gattaatgat cggattttta cctgattttt
    19501 tatataattc atctttttta cgattaatca cttcgcgcat tgacgtgaat tcagttgatc
    19561 ctaaagcccc tgcttgtaat aaataatctt gcaattgttt ttcatttagg tttctatgaa
    19621 tgtcttgaag ccctagtaca tcaaatgaaa agataccttg atatgtcttt ttagaaatat
    19681 aattaagttt cttttgtaac caagcatcat cacgcacagc accattaggt aaatatactt
    19741 tcacatcacc ttgagcactg cctttaattc gttcaacttc aatctctaaa ccatcatcaa
    19801 gaataagtac taatttacca ccgtattggt tacctagacg tggttctagt cttggctctt
    19861 tagacttttt agttggaaaa ccaaataata tcgaatggat gaatgcttga atcgtcgatt
    19921 tacccgcttc attttcacca aaaatttcag tgaagttttt attaaattca attttacgtt
    19981 gaacaaattg accgtaacca taaatttcaa gtgatttaat tatcattttg ttcacctctc
    20041 atttcagctt ttaatatttc ttcagcacga ttaactaatg ctgtatggtc gaatgttcca
    20101 taatcgtcta ggaacttaga tgcccttgga tttaaatata aatctgacat cgctttatca
    20161 aaaacagttt gatcgactaa taattccgct gaaaattcat taactaaagg tgactcatca
    20221 ttttgtgcat attgtatttt taactcatca atatatacaa attgattttc gttttcttca
    20281 taatctgtaa tcatttcttc aacttgtaat aaatcttgag gtgaaattaa tgtctcacta
    20341 ttaataacaa gcgttaaacg ataaaaggct tttccttctt ctctcacttg ttctttaaag
    20401 ttttgaatga cctcgtataa accttgctta gatgtcttat ccgtttcaat agttgcttct
    20461 tcaaatctaa tatactgtgt aggataaaat ttagttttta attttaagtg gtcaccctcg
    20521 attaataagc aacctttttc accttgctca ttaaaatgtc taccttgaat attacctgaa
    20581 tagttaatta caggcatatc acttaattgt tgacgttcat gtatatgacc taaagcccaa
    20641 taatgataca atttactgtt taaatcttct aaaatgaact cggtatatct ttcgttaact
    20701 gaagatttac tatacgtacc atgcaagaca ccaatatgta tgcctttttg tccttgactt
    20761 gatggatatt catcaatctt gttctcataa cttgctctat tttcataact aaatccgtga
    20821 atataaattg tttcaccaga tttagtaatt gcttcatacg tctcaacttt atttgaaaat
    20881 acagaaacat tatctggcca gtttgatgaa atctttgatg ataatgggtc gtgattacca
    20941 tgacaaacat aaacaaagat ttgttcattt tgtaaacgtt caaattgctg ttttaagaaa
    21001 atttcagcac gtaatgttct gttttcacta tcaaataaat cacccgcgat aattacaaaa
    21061 tcaacatctt gttgtaatgc aatatctaca atgtttttaa aactttcata agcacttttt
    21121 tgaacatcct caaaaatttt agggcttata tgactcttag atttgaaagg actatctaaa
    21181 tgtaaatccg aacaatgaat aaatttaacc atacatccat atctccttaa ttaaaggttc
    21241 taacatcaat tttgcaaaat ccaactatac aaagcattct aatcactata ttcatactta
    21301 taggtgtagt gattcattag ctgatttatt aaaaccagtc gccattttta tttcgttaaa
    21361 aaattattat ctacacaata ataaagtgct ttagtcaaat cgtgatgttt tatttattaa
    21421 caagtttatt atttcttcat tttaccataa tacgcttcat atcgtcgatg aacatatgaa
    21481 attgagacac atttataaca gtttaaaatt ttatttgtag cgtacgttga tttttttcaa
    21541 aaatttcagt cgaaaatgca atttccgtaa ttgttaacac tgctagtgaa ccaactgata
    21601 cattaaagat ttacatgatt actctactag ttctgctcgg cagtaatgac caattagaag
    21661 ctaaatgtca aaactatcat tgcacttaat acaccaaaca ttttcaggcc gttcttaatg
    21721 agtcattaaa agtgaaacac ttttccatca gtccgataca tgaccgaact aatagtatta
    21781 tgttgaaaac tttacactcc aaaatgcaaa aaagaagtta ggacacgttt actagcctaa
    21841 cttccgatac tgaacgcatt tgcccaattt gacatagaac ctctaacttc tgttatcaaa
    21901 actaaaaaaa ctacccacaa agtctcggac tctgtcggca gtctattccc ctattaaaag
    21961 gattaaaaaa tattatttta atattagtca gcgtaaattt cgtctaatgg tttaacgata
    22021 atttggttga tttcttggaa tacttgactc attttttgtt cagcattcat taatgcagag
    22081 atgttttcat ctttttcaat tgcttgcgct tgttcttgcg ctttttgtaa atcttcttca
    22141 gcaatttctt caccttgcat ttgtttttgt tggaagttaa tttgagtttc acggaactcg
    22201 tcgaataact ttttagattc ttcgttagct tttacattag cgaatgcttc tttgattgct
    22261 ttgtattctt cgctttctct taaagcttgt tctaattgat ttgcataatc atataaattt
    22321 actgccatgg ttatagcact cctttgttgt gatgtttatt aaaacgttcg atattacttt
    22381 accacatctc gcttttttag acaaagattg caaccaaacc ttggaaaaat ccaataatac
    22441 cacctaaaat gaaacctaat gacataatca actttagttc tttgttagca atttctacaa
    22501 ttaatttttc aatataatct aagtcaaacg tattaatttg ttcttcaatt aatcctcgta
    22561 aatccacttt tttcataatt gtagataagt gaatcgataa ttgttcaatg attaaatttg
    22621 ctaatttgct agagagttga ccttctaaat aatcaacgaa ttgaggcatt aacgtaacca
    22681 ctggcttatt cgcttgtttg cttgcatatg ttgttacata cactgataga ttttcagcga
    22741 tttcattaaa ctgagacgca tctaacagtt cattcaatgg tctatcttta aaagtttgat
    22801 attcattggt aattaacgat gtcacaattg ttcttgcttt aggatgagat gttaaacgta
    22861 taagttcttg ttgaatgcga tctgcaatgc tctcttttgt cataaacatt tgcaacatac
    22921 caattaactt acctttctca ttgaaaaaag tatccaacat atcattaata tcttgtgtgc
    22981 cttttacaga tgataaatat tttcttgcac gatcacataa taagtctgtt gcgttatcta
    23041 catgctgatc taaaaatgtt acaagttgat ttggcaataa agatgctatt gtttggtttt
    23101 gatgatttgt ataataatta ttcaattgtg attcaatata ttgatttcca ttagtttgta
    23161 atacttgttc taaatcaata tcgatttgag aagtaatttg ttttattgac aattgatctt
    23221 tcgtcaactt ttgtaactgc tgttgaatca tagattctat tgcttgctgt gattgctcgc
    23281 tttttaattt ttcattaatt aaagtttctg taagcaaatg ctcttcaatc acttggccaa
    23341 ttttagttgc aatttcttcg cgtcttttcg gtattaaacc tggtgtaaat gggactctaa
    23401 atttaaatat atagtatggt ttaaaggggt gaaacagcat tctaattgca attacattag
    23461 taatgccacc aattatcgcc cctactacaa tcataaatat gataataaat agtgcattca
    23521 taattttgac tcctttgtga caacatagtt attcattata ccactatttt tattttttaa
    23581 ttcattaatc atttcacttt ctgctcaatt tattattagt ttacatacaa aaagcgatgc
    23641 ctcacctctc gaaacaggtg tgttacatcg cttttattat ttattaaaag tatataggcc
    23701 tagatatgta ctaattcttc tttagcattt ctgttaaagt atgtttcagc ttctctcatt
    23761 ttagaagtac cgaaaattgg ttggttatct ggatttaata cacgataaat attgcttact
    23821 ttgttacttt gtaaaatgta cccattacga gtctcaatgt tattccaata aatatcactt
    23881 gttggtgcat ggtttggata tgcacagtga tttcttgaaa ttgtttgaac gatttctaat
    23941 ggatcacagc caaatgtact gtttaatact tcagagtctc tgaataatgc acaaattgaa
    24001 atacaagttg tccagtttgg taatactctc tctttttcga tttgtactaa agtcttttta
    24061 gaaagtccaa ttgtttgcgc catagtatct tgcgtataac cagcctctat acgaaccatt
    24121 ttaaattttg tttgaattaa atctgtaaaa ctctgtctat ccattctgtt atctaccttt
    24181 ctgtttgggg aattttatcc ggacacaaga aattgcaata atacacattt cttgaaacac
    24241 agattacatc ttaatatatt tttaataaaa tgaaaagagt caatttcaca tttgtattaa
    24301 attttgatca agtcaacaag agttaagttt actttataat gaagcgaaag taaatgaagt
    24361 aaaattttat gcataatcac tagatttgat aaaacttaca cttttataat gatatcaaat
    24421 ttacaaagaa aactgtataa taaaccctat cgctataaaa aatcatcaaa atcagattaa
    24481 aaattgtcta tttttcgaca aatatttaaa agctcattct aaatcaactt attttccatt
    24541 gcataaattg ctgcttgtgt acgatcgcta acttgtaatt tactaaatat atgactgaca
    24601 tgtgttttaa ttgttttttc agatacaaat aaagtttctg caatctcttt atttgtttta
    24661 cctttaacca tttcacgtaa cacttcaatt tctctctttg acaacttatt cgtgtagtgt
    24721 ggtttttggc taactgtttc gaatacatct tgtgccttag gatgtatcat tttttcaccg
    24781 ttcataactc gtctaatagt ttcaattaat tgctgaggct caacgtcttt catttcataa
    24841 ccatcagcac ctttattgat tgctgaaatt acatgttcat catcaacata acttgttaat
    24901 accaaaactt taatatccgg ataatgtgcc ttaatatatt ccgtaatttc aataccattc
    24961 atgccaggca tcactaaatc taatagcaca atatcagggt gctcatgctc ttttaaatat
    25021 tctaaaaatg tttctccatc tgcaaagtct tgtaaaactt ctatgttttc aatcgtggat
    25081 aataaaaatc gcaatccttg tcgcacaata taatggtcat ctactaatat tactttgttc
    25141 atgggttatc tccttaaatc aagctatttt ataggaattg tgaattgtat ttgtgtaccc
    25201 tttgttggct gagaatgaaa ggtcacttta cctcttaata atttaactct ttgttttatg
    25261 ttatttatac cgtgtgatga agctatctga acattatcga tctcaaatcc ttgaccataa
    25321 tcaatcacgt caatatatag tatatcgttc atttgtttta atgtaagatc cattttattc
    25381 gtatcagcat gtttcttaac attattaata cactcttgta atgctctgta tatgttttct
    25441 tcgatttcat tagataaatc gattaaacct tctacattta catttaattg tatatgcatt
    25501 aatttactat atgctgtcaa agcatgaatt aagccttgct caagtccaac tggcttaagt
    25561 tgccaaatca atgcacgcat ttcattaacg gcattttgac tcgtttcctc aatcgtcttg
    25621 aatgcttgtt tagcgatgga ttcgtttgac atgccatacg cagcatgtgc tgttagtttt
    25681 acagaaaata acatttgatt tactgaatca tgtaaatctc tagctaaacg attacgttca
    25741 ttaatttttg ctgcttcttt ttcacggtct gttaaataaa tacgtttgat ggctgaccct
    25801 aattgaaatg cgacagactc tagcaactct aaatcttcat cactatatat ttcagtattt
    25861 ggtgacgcta catttaaaat gccgaattgt tcttgacccg atttaagtgg taccgttgca
    25921 tgatgtgtaa tattgtcatt ttggctagga aatgctttag aggctaagtt aatacgagaa
    25981 caattgacga tattcgacgc tttcattagc ctacgttgat taaatgcttt cacacaccaa
    26041 caagacccat ctttaatata gtgacagtgg tctgctgtca aagattgtgg tagagccaca
    26101 tgtgatacaa gttcatgttc acctacgcta ttgatgaaaa atatccagcc tgtcgtgaaa
    26161 ttactgccct caattaaata ttttaacgca ccttgggtca tgctatacat ttcagtttct
    26221 tcgtttaaaa attcggcaat ctcttttaat aaagctagtc gcgtcctttg ttccatcaaa
    26281 tcgctccaat tcatttttac gtgtattaac tattatacat tgagttatta tatttttaaa
    26341 tcttagacgt gaacatgata aaatggcctt gattactcaa tagttatatt tcggagaact
    26401 gatttgtgat atgatattaa agactatagg aggattttat gaaatttaaa ataccagaaa
    26461 actttaatga cttaagttta cgagatattt tccaacaact taaggtacct aaaaaagatt
    26521 tgcatcattt aaatatgtct aaagatatta ctattaatga taaacctgcg cgattaatgg
    26581 ataaagtgca tactggcgac gatgtatttg ttccaaccat tgatgaaaaa agtaattatg
    26641 ttccaagtta tcgttatgca caaattaaat acgaagacga tgatatggca atcgtaatga
    26701 aacctaaagg tgttaagacc caccctaatg atttaaaaga aagcaatact ttaatgaatc
    26761 atgtgattta cacggttgat agtgactatg tcgaaccaat tcatcgactg gaccaggaaa
    26821 cagtaggatt attaattgtt gctaaaaatc ctttaatgaa aaaaattctt gatcgcatgt
    26881 tagaagacaa tgatattacg cggatataca aagcaaatgt taaggcactt ttacctttaa
    26941 aaccacaaac gattgatatg ccaattggta aagataaatt ccattcgaat aaacgacgtg
    27001 tgtctcctac tggacagcgt gcaattacac acattttaac ttcaaaaatg ataaaagaaa
    27061 ctgtgtgcca acttgaaatc aagttggata ctggacgtac tcatcaaatt cgtgtgcatt
    27121 tagctgaaat tggtcaccca gttattggtg atcctttata cggtgattca acgttaagac
    27181 aattagaact tgaaagttac aaaatagagt ttgtgcatcc cttgactaag gaagtcattt
    27241 ccatttcttt ggatgactaa tttgtttagt ttggcatgat atttaaatat gcaatactgc
    27301 attgtaacta aatcaagtat caacttaaat cggatagatg gaaaattatt aattttttca
    27361 aatgttcggt ttttttatgt tttttacgat gcttaggatt ttatattttg atattttagt
    27421 aatttttcat tttatagcat ccttggataa tgacttgtag tctttttcaa ctgcgttacg
    27481 tgtttctatg gacaatacat gacatcataa gatttttatc acaggttgtt tggccaatac
    27541 atgtacaaca attcatctta taaaaaatag gttctataat aaaacggact ccatgaaaag
    27601 ttttttcttt catggctcta tatcaaatcg gacttataag ttcaatttga catagacaaa
    27661 aaataaagct tgaacactac atctacctgt aatgcccaaa ctttatttta tattaatatt
    27721 taattaatga ggatctacca tatcttctgg tttaatccat gcttcaaatt gttcttctgt
    27781 aacatatcca gtttgaattg cagattcttt taaagttaaa ccttctttat gggctttctt
    27841 agcaatttga gcagcttttt cataaccaat atgtggattt aatgcagtaa ctaacattaa
    27901 tgattgattt aaataattat caatattctc ttcgattggt tcaatgccca ctgcacaatt
    27961 gttattaaat gtttccatac cgtcagctaa aaggtaaatt gattgtagtg tattatgcat
    28021 aataactggt ttataaacat tcaattcaaa gttaccttgt gaacttgcga agccaacaac
    28081 tgtatcatta cccattactt ggactgcaac cattgttaac atttcacatt gtgtaggatt
    28141 aactttacca ggcataattg atgaacctgg ttcattttca gggatagaaa tttctgccaa
    28201 accagctcgt ggccctgaag ccaaccatct cacatcatta gcaattttca ttaagtctcc
    28261 tgctagcgcc ttcaatgttc catgtagttg tacaacctca tcatgtgctg ttaatgcatg
    28321 gaatttattt tcagaagata caaatggata acccatattt tctgaaatat aatgtgccac
    28381 tttatcacca aattcaggat gcgcattaat accagtacca acagccgtac caccgatggc
    28441 aagatttaaa atgtgcttct tagattcaga taacattatt tcgcaacggt caagcatata
    28501 acgccagcca ctaatctctt gtcctacttt gatcggcgtt gcatcttgta aatgtgtacg
    28561 accaatttta ataattgaat caaatttatc ttctttttct ttcaaagtat ttcttaaaag
    28621 ttttaatgta ggttctaatt ttgtttcaac ctcttgatat aatgcaacgt gcatagcagt
    28681 tgggaatgta tcattcgaac tttgtgattt atttacatca tcatttgggt ggatactttc
    28741 atcactttga tgatctttta aatacatatt agcaacataa cttactactt cgttcacatt
    28801 catattactt tgtgtaccgc ttcctgtttg ccatacaact agtgggaagt gttcatctaa
    28861 ttcacctgat aaaatttgat cacatgcgta tacaatggca tctttctttg ccttgcttaa
    28921 ttttcctaaa tcaaaattag ctaatgctgc tgcacgcttt agttgtgcaa aaccataaac
    28981 tacttcgatt ggcatacgct ctttaccaac tgggaaatta cgtttacttc tttctgtttg
    29041 agcaccccaa tatttatctg caggtacttc tatttctcca aaagtatcat gttcaattct
    29101 tactgacatt caatttctcc ccttatcact gtttatttaa ctgtagtata tcattaaata
    29161 atttaattga gcaatttatg attaaaacgt tttcataatt tgaaataaaa atacactaat
    29221 cgcacgtact caccctttat taaagtgata cggtcatacg attagtgtgt tatctatcat
    29281 tatttagtta ttgttgaact aagtttaatt acgatacttt gttttagtag cttcaaccgt
    29341 agcaatagct gtaagtatat ataatacagc actaacaatt gtcgtatatg gatttagaac
    29401 aacaagcgta cctaatactc ctgttaaact cgcataaaac ccatcaagtg tataatacaa
    29461 tattcctgtt attaataaac aacaaattac acttattaca atacctgatt gattactttt
    29521 aatgaatgtt tgcgcattaa catcatcgat taatcctttt gataaattga gttgtaattt
    29581 tattactttg aaaataacag gtaaatataa tgccccaatt gccaatggaa aagctttaat
    29641 tgatattaaa cttataataa ctgttgctat caataattga atccagtatt ttcctaacat
    29701 aaatatataa atctcctcta atttcattct tcaatagcat atcataatct tggcatatta
    29761 agtaatgcgg tttaatgatt tcattaaaaa tattaatgat agatgacttc tttcaattat
    29821 aactgggcta attaattatc aatttcgttt aaatgttgtt ttaatattta aaattgaatt
    29881 tttgatatat tactatgtct ggtacacaaa tcaatgtttt atgctttaca aagttatatt
    29941 ggcagtagtt gactgcaggc ctcaaacaca gaaactggcg gaaagtcagc ttactatttc
    30001 atgcaagttg gcagggccac aacacagtga aatcggattt ccaatttcta cagacattgc
    30061 aagttgacgg agccacaaca tagtgaaatc ggattcccaa tttctacaga cattgcaagt
    30121 tgggggaacg ggccacaaac tcagaagctg gcggaaagtc agcttacaat aacgtacaag
    30181 ttggcggggc tccaacatag tgaaattgga tttctaattt ctacagacat tgcaaatttg
    30241 tggaaacggg ccgaacacag cgaaattgga tttctaattt ctacagacga tgcaagttga
    30301 cggggccaca aactcagaag ctggtggaaa gtcagcttct atgaatataa taaaaaagct
    30361 aggtaacaaa atgctaccta acttcatatt caagataatc aatcctattt gatatatgtt
    30421 ctatactata cattgtttac atgataaata gctgaatatt acacaattat aatactttac
    30481 tgactgtctt cttcagaatt cttttcttga tcattctgat cagagatttg ttccatttct
    30541 ttacctaatt cttttaaatc ttcaaaatca gttaccatac tgttttcttc ttcatgataa
    30601 tttaattttg gatctttgtc tttagacata atcaatacct cacagatttt taattaagca
    30661 aagcgtgacg taaagtaagc tttaacatct tcaggtaaac ctgcagccgc ttctttatca
    30721 agaataacat ttaccattct atgtgctttt aaatcggctg gttcgaagct tgttttacca
    30781 ttttcttgat ataatttttc aactacatct cgtttattag cacctgtcac tactaagaaa
    30841 atttctcttg cttccattag tccttgacga atactaacat ttaacttacc ttgctcatcg
    30901 atagagacaa cttgtaatgt taatttccct ttattttctt tagttttaat cttatcagcg
    30961 attaattcga ttgcatcttt ttcataagca attggataaa cttgacctgc tggtatacct
    31021 aacgcttcga aatatgattt tttatcgtca taatctaaaa tatttatttg gctaaaatca
    31081 acagcatgtt tttcaatatt tttctttaat tcatctagta ctggcgcttg atcagtatct
    31141 aaatgaaaac ctgcaattgt agtaggatta ttgttaaatt gctttctaat aatatcagca
    31201 gcatattctg ctacaagttg actattgtca aagactttaa agttcattgc catgatttct
    31261 acactcctct tagtttgcat tttacataaa aacggcgata ttaataatta ttatacccta
    31321 actttcaata tatcaaacca tttaacttta acatgcttat actctaaata tagcacttaa
    31381 gcatcatttt tataatgaaa atgagtaaat tttaattcaa tcctggaaaa ttttgttgac
    31441 gtaacgcttc ataaattaac aacgcagcag tatttgataa atttaatgaa cgaatatgtt
    31501 cactcatagg aattcttaac gctgtgtctt gatatttctc tttcacccag tctggtaatc
    31561 ctgttgtttc ttttccaaaa atgaagtaaa aatctttgtc atgatttgaa aaatcaaaat
    31621 cactataagt ctttttacca aattttgtta ataagtaata ctcgccattt gtggtttcaa
    31681 aaaatgcttc aatactatca tgatacgtaa tattcacaaa ttcccaataa tctaaaccgg
    31741 ctctttttaa cattttatca tcagttctaa atccaagagg tttaattaaa tgtaaatgtg
    31801 tgtttgtacc tgcacacgta cgtgcaatgt taccagtatt agctgggatt tctggttgat
    31861 ataaaacgat atgatttgtc atattactat tctctccttg tgtctaatcc ttttatcatt
    31921 tcattctgaa cttctgcatc ctcttgatca taatttgcat tgataaaatc tcttgcttct
    31981 tccccaagaa tttgaccaat agcccaataa gcagttgctc gaatcatcgg tctttcatct
    32041 gttgttgcaa cttttttcaa ttctggaatt gcatccactt cattaaaatg cgccaatgct
    32101 aaaatagcat ttcgttgtat cggtttttta ccacgccaag cacctgcaag gtgaccatat
    32161 gtttgtttga attctttatt agacatacgt agtaaaggta ctaatcttgg ctttaaaatt
    32221 tctggttcca aaatgatgtc atcttgttcg gtattaatac ctctattttt cgggcaaact
    32281 tgttgacacg tatcgcaacc atataatcta ttcccaattt tataacgata ttggtcaggc
    32341 atatagcctt ttgtttgcgt taaaaaacta atgcatttct gactatttaa ttggccattt
    32401 ccaactaatg cacttgttgg acaacgatca acacaaattg tacaatcacc acagctatct
    32461 aataatggat catcaggttc aaaaggtata ctgactaaca tttcaccgag gtatgtccat
    32521 gttcctagtt taggattgat gacaaagcca ttacgaccaa caaatcctaa acctgcacgt
    32581 tctgctactg ccctatctga taatacaccc gtatctacca tagatttgat ttcaacatct
    32641 ggaactttag attcaataaa tgcagctaac atgtctaatc gtttacgcat aattgtatga
    32701 taatcttgac cccacgatgc tctagcaaat aagcctctgc gatcacctct aacactctta
    32761 ggtgcacctt tcagtttgtt aggataacca actgcaattg ctatgattga ccttgctgtt
    32821 ggtaaggata atttaggctc cgttcgtaaa gcagtatcag attcttcaaa tcctgaggca
    32881 taaccatttg catgatatgc ttctagcttt tgcttcaatt catcaaaggg atcggcagta
    32941 gtaaatccaa tactgtcgat accaattgta tatgcatagt caatgatatc ttgctttaac
    33001 tgctttgtat ccaatcgtat gccccccata aaataatact atgttatttt aacacaacca
    33061 ttattcatat tgaggtttca tttcacataa ttttaataat aaaaagcttg aatatatgat
    33121 ataactttgt ggtgcgcatc atatattcaa gcttcgttag gttgttataa tactcttgct
    33181 aagaaatttt gtgttctttc atgttgcggt tgttcaaata tttcgactgg tgtgcctgac
    33241 tctacgacaa cgccatctgc cataaatatg actttgtcac ttacatcttt ggcaaatccc
    33301 atttcatgtg tcacaaccac catggtcata ccttctttgg ctaggtcttt cattactttt
    33361 aatacatcac caactacctc aggatctaat gctgaagttg gttcatcgaa taaaataaca
    33421 tctggatgca ttgctaaagc tcttgcaatt gctacccttt gtttttgacc acctgataat
    33481 tgattcggat atacatctgc tttttctttt aatcccactt tatctaataa cgacaatgct
    33541 tccttatgta attcatcgtt attatctttc tttaataatt taggggctaa aataatatta
    33601 tcgacaactt ttttatgtgg aaataggttg aagttttgaa ataccatacc cattttttga
    33661 cgtagtttat ctacttgtgt ccctttttcc gttaagtcat tgccttcaaa aatcacttga
    33721 cctttagtgg gtacttctaa taaattcata catcttaaca atgtactttt accactacca
    33781 gatggaccta ttattgctac tacttcccct tgattgattt caagattgat atcttttaaa
    33841 acttcattat ctccaaaaac tttattaaga ttgtttattt taatcactgg cattcattct
    33901 cccttcaatc atgttcataa tgcgtgtaag tacaaatgtt aagacaaagt ataatgctgc
    33961 tgccactagt aatggtgtga atgggtcaaa tgaaatacct tgaaccactt gtgcattaaa
    34021 cataatttcg ccaactccaa ttgttgacac aatagatgat tctttaatta aagtgacaaa
    34081 ttcattacct aaagctggta aaatgttttt aattgcttgt ggcataatta cacttttcat
    34141 cgtttgtcta taattcaaac ctagactacg tgcggcttcc atttgtcctt tatcaacagc
    34201 atttatacct gcacgaataa tttcagcaat gtaagctgag gaattaataa ctaatgcaat
    34261 tgttccacaa acaagagctg aaatgtctag tcctaatgca gcagttatac caaagaatac
    34321 gataaatact tgaactaaca ttggtgttcc tcttaatatt tcgatataga tagaggcaat
    34381 ccatgaaata atttttattt tacttaattt cattaacgca acgaatgcac ctaaaataga
    34441 acctaatgca acaccgataa gtgaaattaa tattgtaatc ttaattcctt tcaagaaaaa
    34501 actaccatac ttagaaataa aaccactgtc atcattcatc gcattcgcag cattagtcat
    34561 atatttatcg attaatcctt tatctttaac ctctttaatc gttttattaa tttgtgacaa
    34621 taattttggt gaatcttttg gcactgctat cactgtatct ttttcttctt cattaaattt
    34681 cacattggaa attcctaatt taggattttg ttttaaatat gcttctgcta caggtttttc
    34741 aactacagca ccttcaacct ttccactttt cagagctaat ataacgtctg gtaaacggct
    34801 taatgaagta atagatgcat tttcaatttc ggtttgagcg attttttctt gttcagttcc
    34861 cttttgtgcc cctacttttt tattattaaa gtctttgata tctttatatt cattaacttt
    34921 atctttcttt acaagcatga tatttttagt catcatatat gaatctgaaa aatcaacttg
    34981 cttcttacgt tcaggcgttg aagtcattcc ggaaataata atatcaattt ttccagtttt
    35041 aagagctcct aacaaactat caaatgacat attgacgatt tttaatttta aattattatc
    35101 tttcgcaatt tttttagcta aatcaatatc tacacctgca tactcagtct taccattaac
    35161 tgtatgctca aattccattg gtgcataatc tgcagaaaga cccactctaa gttcaccgcg
    35221 ttctttaatc ttctcccatg tttgatcttg ttcagcatgt gctgtgggat tgatatacac
    35281 catagcactg ctaattaaca accctaatac taatataaac ctaattaaac acttcattta
    35341 tacagccctc cctaattaat attcataatt atacatatta atacattttg atgcaatagt
    35401 gttttataaa tgtattttta tcaatataaa gaaaagcttc tttttaaaag actgtactta
    35461 aaaaatcgcc tattatacgc gaacaatcct tacattttaa aatcaatcaa aaaaagcgct
    35521 actatcaata tcttattgac agtaacgcct atatttatca tatatcaaaa cttattcttg
    35581 tcatcaagct ataaattatt gttttggaac ttcaaattta taaatatcat tggaatatat
    35641 aggaatgaat tttttacaca attcatgaac aataaacgcc gatacaaatg gtatgacaaa
    35701 ataaccaaga gctaataaaa taatattcat tgttggatca cctgacattc ggttaaatgc
    35761 atttattggg ccaactaatc ctgtatatcc aaatccagca gacaatggtg ttcctttaac
    35821 ctgaagaacg taagcaatga ttccagtaat tattccattt atagtcaatg gtatcgaaat
    35881 aattaaattt ttcaaataaa cagggatcat catttttgca gctcctatga gtaaaactgc
    35941 atttacgcca atagaattga cacgcaatga gccaaataag aaagtaacac acgcagccac
    36001 aatacctagg tttgctgctc cacttcctag tccgtttaaa ctaatcgcag ttgcaatcgc
    36061 tactaacgat attggtgtta ccattaataa tgaaaatgcc acactaataa gtatagacat
    36121 taacaacgga tttaagtctg taaaagaatg aattacattt ccaattgctt gagtaatttt
    36181 tcgaatgtaa ggtaatgtga ttagaccgat acccccacta acgataggta ctaaaactgg
    36241 taatataatt aattcaaaag atccaagttt gttttgtaat atcatatata taagacatgc
    36301 aataataaca accaaacttg tatttataat gtcacctata ccttttaaca taaaactatt
    36361 attgctatat acaacagcac ctgaaccaat catagctgat gtacctacta tagcagcacc
    36421 tgcgccatta aatttaaatt gatgagcagc taaaacccca ataataaatg ccataaatga
    36481 ttgaattagt atcactaact gatacgttaa ttctaaaatt tcattaccac ttttaaatat
    36541 ttttaatact tcacctaata aagcattcgg aacaagtgca ataacaacac cagcaccaat
    36601 agaatttaaa atcttactga aaaactgttt attatcagca ttctttgcgt tactcataaa
    36661 cgacctccaa tttgaacatt aaactcatca tattatagaa aattaacttt aacaatgatt
    36721 agttggctaa tttaatctaa atataacaat ccacaaaagg aataaagtaa atttgaacaa
    36781 tctactcagt aaacaatgtc atgttaattt taaaatacta ctaattacat cctgtgacta
    36841 ataatgtaat aaattgtaaa tattaaaata gccatattac ttttaagata gataaattga
    36901 ttcgacttgg tataaagcag attaaaatag cacttttaca aatactttat tagaaaagtt
    36961 aaaacaaata cataacccca gataatatag tcattatca
//
LOCUS       DAHHRR010000011        36880 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.011, whole genome shotgun sequence.
ACCESSION   DAHHRR010000011 DAHHRR010000000
VERSION     DAHHRR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36880
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.011"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            42..1097
                     /locus_tag="NBI42_000478"
     CDS             42..1097
                     /locus_tag="NBI42_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCD1776927.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEINKIRKAAELADKCIEIGVSYLKEG
                     VTEREVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPNKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            complement(1176..1289)
                     /locus_tag="NBI42_000479"
     CDS             complement(1176..1289)
                     /locus_tag="NBI42_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776928.1"
                     /translation="MNNIDLIHYMLLMNIPAYVFLACLLYIAVRLGQKFMF"
     gene            complement(1413..2531)
                     /gene="ald"
                     /locus_tag="NBI42_000480"
     CDS             complement(1413..2531)
                     /gene="ald"
                     /locus_tag="NBI42_000480"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCD1776929.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            2672..3172
                     /locus_tag="NBI42_000481"
     CDS             2672..3172
                     /locus_tag="NBI42_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD1776930.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            complement(3418..4620)
                     /locus_tag="NBI42_000482"
     CDS             complement(3418..4620)
                     /locus_tag="NBI42_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCD1776931.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(4708..5655)
                     /locus_tag="NBI42_000483"
     CDS             complement(4708..5655)
                     /locus_tag="NBI42_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1776932.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKNPSQFQGFMTELNQWMDTNRPKK"
     gene            complement(5778..6272)
                     /gene="tpx"
                     /locus_tag="NBI42_000484"
     CDS             complement(5778..6272)
                     /gene="tpx"
                     /locus_tag="NBI42_000484"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCD1776933.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSEASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALTA
                     YKNI"
     gene            complement(6370..7140)
                     /locus_tag="NBI42_000485"
     CDS             complement(6370..7140)
                     /locus_tag="NBI42_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCD1776934.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAILFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMATSMIAGSY
                     TGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            complement(7184..8407)
                     /gene="thiI"
                     /locus_tag="NBI42_000486"
     CDS             complement(7184..8407)
                     /gene="thiI"
                     /locus_tag="NBI42_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCD1776935.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTLEAMSAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVAGAGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            complement(8407..9555)
                     /locus_tag="NBI42_000487"
     CDS             complement(8407..9555)
                     /locus_tag="NBI42_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD1776936.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSIYYNPNSPHKAGLQA
                     NQLLQQAKAQINAMINSKTNYDVVFTSGATESNNIALKGVSYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAEVIKNYPKAHFHVDVVQAFGKISMDLTNVDSVSLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENLEALNAFVTELNNDVRQF
                     LNKYQGVYINSSASGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            9704..9844
                     /locus_tag="NBI42_000488"
                     /pseudo
     CDS             9704..9844
                     /locus_tag="NBI42_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9885..11579)
                     /gene="ezrA"
                     /locus_tag="NBI42_000489"
     CDS             complement(9885..11579)
                     /gene="ezrA"
                     /locus_tag="NBI42_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCD1776937.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            11716..12180
                     /locus_tag="NBI42_000490"
     CDS             11716..12180
                     /locus_tag="NBI42_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCD1776938.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQV
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQVVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            12424..13026
                     /gene="rpsD"
                     /locus_tag="NBI42_000491"
     CDS             12424..13026
                     /gene="rpsD"
                     /locus_tag="NBI42_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCD1776939.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(13242..13985)
                     /locus_tag="NBI42_000492"
     CDS             complement(13242..13985)
                     /locus_tag="NBI42_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD1776940.1"
                     /translation="MTLNKLKDELQIVSHRGLPSAFPENTMSSYRQAMELDIDMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKFDKKLLIELKSPNLYPGIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDEAHHHQLQVMPY
                     TVNKLKAGEKLRQMGVDGLITDNPKLFIK"
     gene            14065..14511
                     /locus_tag="NBI42_000493"
     CDS             14065..14511
                     /locus_tag="NBI42_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCD1776941.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPHI
                     EIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            14626..15786
                     /locus_tag="NBI42_000494"
     CDS             14626..15786
                     /locus_tag="NBI42_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCD1776942.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINYLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            15773..17377
                     /locus_tag="NBI42_000495"
     CDS             15773..17377
                     /locus_tag="NBI42_000495"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCD1776943.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHSLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAD
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            17471..17566
                     /locus_tag="NBI42_000496"
     CDS             17471..17566
                     /locus_tag="NBI42_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776944.1"
                     /translation="MKPLFQSFTSSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(17527..18651)
                     /locus_tag="NBI42_000497"
     CDS             complement(17527..18651)
                     /locus_tag="NBI42_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1776945.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKWQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFAITELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEEVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGPYFEADFIATASDVLEAEKMYPQ
                     ARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKIGA
                     TFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(18758..20224)
                     /locus_tag="NBI42_000498"
     CDS             complement(18758..20224)
                     /locus_tag="NBI42_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HCD1776946.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHAAPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAAALGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFVYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGIVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHNHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQ
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            complement(20352..20969)
                     /locus_tag="NBI42_000499"
     CDS             complement(20352..20969)
                     /locus_tag="NBI42_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCD1776947.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            21141..22415
                     /locus_tag="NBI42_000500"
     CDS             21141..22415
                     /locus_tag="NBI42_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCD1776948.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            complement(22509..23771)
                     /locus_tag="NBI42_000501"
     CDS             complement(22509..23771)
                     /locus_tag="NBI42_000501"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCD1776949.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            24152..25057
                     /locus_tag="NBI42_000502"
     CDS             24152..25057
                     /locus_tag="NBI42_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD1776950.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            complement(25037..25129)
                     /locus_tag="NBI42_000503"
     CDS             complement(25037..25129)
                     /locus_tag="NBI42_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776951.1"
                     /translation="MKINLLLFLIFGCIVVVTLKSEHQLTLFSI"
     gene            complement(25263..27944)
                     /gene="harA"
                     /locus_tag="NBI42_000504"
     CDS             complement(25263..27944)
                     /gene="harA"
                     /locus_tag="NBI42_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HCD1776952.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKSYPAADESLKDAIKNPAVENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGAETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVATNDQSSSDASNQTNTNTSNQNTSTTNNANNQPQATTD
                     MSQPAQPKSSVNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDIKTKDDDTSQNNTSEPLNVQTGQEDNVT
                     ATDTATNNSIETTPSEATDKVDVIEPESDMVKDTDSSVDKDAHLNIDHSSDMSGNTHF
                     DKYDLKEMDTQIAKDTDKGVVNSVGMSSNVDTDKGSNKNKDKVIQLAHIADKNKVNNT
                     GTETNIDTMKYHPISTIKVTDKKTTEHLPSDIHKTVDKTVKAKEKAGTPSKENKLSQS
                     KMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            27943..28032
                     /locus_tag="NBI42_000505"
                     /pseudo
     CDS             27943..28032
                     /locus_tag="NBI42_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500349.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28277..29944)
                     /locus_tag="NBI42_000506"
     CDS             complement(28277..29944)
                     /locus_tag="NBI42_000506"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCD1776953.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRDSKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELKKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVQGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGVIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(30384..32090)
                     /gene="acsA"
                     /locus_tag="NBI42_000507"
     CDS             complement(30384..32090)
                     /gene="acsA"
                     /locus_tag="NBI42_000507"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCD1776954.1"
                     /translation="MKVEVYKGVQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGSGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGILKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPDLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            32259..32891
                     /locus_tag="NBI42_000508"
     CDS             32259..32891
                     /locus_tag="NBI42_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1776955.1"
                     /translation="MNHLKTYQCEDYYIHGKQFVIEGPLTYEDLKALTFDAHLTAFRD
                     AEDQYEALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGNLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFEDYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            32916..34085
                     /locus_tag="NBI42_000509"
     CDS             32916..34085
                     /locus_tag="NBI42_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HCD1776956.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADTYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(34183..35172)
                     /gene="ccpA"
                     /locus_tag="NBI42_000510"
     CDS             complement(34183..35172)
                     /gene="ccpA"
                     /locus_tag="NBI42_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCD1776957.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLAGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(35245..35358)
                     /locus_tag="NBI42_000511"
     CDS             complement(35245..35358)
                     /locus_tag="NBI42_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776958.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISSFSLNFSKQKYVTVN"
     gene            complement(35712..36803)
                     /locus_tag="NBI42_000512"
     CDS             complement(35712..36803)
                     /locus_tag="NBI42_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HCD1776959.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLLAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT        11976 a         6759 c         5409 g        12736 t
ORIGIN      
        1 cataaaatgt aagtaatatt atgttgagga gtgtttataa aatgacaaaa atatcaaaaa
       61 taatagacga actgaacaat caacaagctg atgcagcatg gattacaaca ccgttgaatg
      121 tatattattt tactggatac cgtagcgaac cccatgaaag attatttgca ttattgatta
      181 agaaagatgg taaacaagta ctattttgtc caaaaatgga agtcgaagaa gtcaaagcat
      241 cacctttcac aggtgaaatc gttggatatt tagacactga aaatcctttt tcactttatc
      301 ctcaaacaat caataaatta ctaattgaaa gcgagcactt aacagtagca cgccaaaaac
      361 aattaatctc tggtttcaat gtcaattcat tcggagatgt tgatttaaca atcaaacaat
      421 tgagaaatat taaatctgaa gatgaaatta acaaaatacg taaagctgct gagttagcag
      481 ataagtgtat cgaaataggt gtttcttatt taaaagaagg tgtgactgaa cgtgaagtag
      541 tcaaccatat tgagcaaact atcaaacaat atggcgtcaa tgaaatgagt tttgatacga
      601 tggttttatt tggagatcat gccgcatcac cccatggcac accaggagat cgcagattaa
      661 aaagcaatga gtatgtacta tttgatttag gtgtaattta tgagcactat tgtagcgata
      721 tgacacgtac catcaaattt ggtgaaccta acaaagaagc acaagaaatt tataatattg
      781 tattagaagc agaaacatct gcaatccaag caattaaacc tggaatacct ttaaaagata
      841 tcgatcatat cgctagaaat attatttcag aaaaaggtta tggtgaatat ttccctcatc
      901 gcttaggtca tggtctagga ttacaagaac atgaatatca agatgtttca agtactaatt
      961 ctaatttgtt agaagctggc atggttatta caatcgaacc aggtatttat gtacctggtg
     1021 ttgcaggtgt aagaattgaa gatgacatac ttgtcactaa tgaaggatat gaagtattaa
     1081 cacattacga aaaataagga gtgggacaaa aatgaaaagc ttgttacaag cgcgttctca
     1141 ttcagtcaaa cactgacaat acaacattgt agcgcctaga acataaattt ttgtccaagt
     1201 ctaactgcaa tatataacaa acaagctaga aacacatatg caggtatgtt catcagtaac
     1261 atgtaatgaa tcaaatcaat attattcatg ttcgatgatt tcttcgcatt gtttctagct
     1321 ttaatttatc attatttaat tttaataacc aaggagatga taacgtcatt ctttagtacg
     1381 ctgtaatcca ttcccttttc attaaattca aattataatt gtaatgcttc ttctacagat
     1441 ttatattcca tttcaaatgc ctctgcaacg cctttattgg ttacgtgacc tttgtaagta
     1501 tttaaaccta atgataatgg ttgatttgat ttaaatgctt ctctataccc tttattagct
     1561 agcatgagcg cataaggtag cgtagcatta tttaaagcta acgtcgaagt acgcggtact
     1621 gcacctggca tatttgcaac tgcataatga accacaccat gcttaatata tgtaggatca
     1681 tcatgtgtcg taattttatc agttgtttca aaaataccgc cttgatcaat agcaatgtca
     1741 ataataactg acccattttt catttgttta atcatgtctt ctgttacaag tcttggcgct
     1801 ttagcacctg gaattaaaac tgcacctatt actaaatcac tttgtttaac atacaactca
     1861 atattcaacg gatttgacat aattgtatgt acacgtccac cgaataaatc atctaattgt
     1921 tgtaaacgct ttggattaac atctaaaatc gtaacatctg cacctagtcc tagtgcaatt
     1981 ttagctgcat ttgttccagc ttgaccacca ccgataatag ttactttacc tttaggtact
     2041 cctggaacgc cacctagtag aattcccata ccaccattaa gtttttgtag gaactctgcg
     2101 ccaacttgag ctgacattct tcctgctacc tcactcattg gtgataacaa tggtaaagat
     2161 cggtctggta actgcacagt ctcatatgca atactaatta cttttctatc tatcaaagct
     2221 tgtgttaatt tttcttcatt tgctaaatga agataagtga ataatacaag cccttcttta
     2281 aaatatggat attcagattc aagtggttct ttaactttaa taaccatatc cacatcccaa
     2341 acttttgctt gttcagcaac aatctcagca cctgcttctt tgtaatctac atcttcaaag
     2401 aacgaccctg aacccgcatt tgtttccact aaaacagtat gcccactttc tactaaagcg
     2461 tgcacaccac ttggtgataa accaacacga ttttcattat ttttaatctc ccttggtata
     2521 ccaattttca taccatccac ctccataatc atcttaacgc gaacattttg aaagcgcaat
     2581 caaaaatcca caaaattgta aaggttatta cactgacttt tccgaaaatt gtggtaaaat
     2641 ataattaaga aagaacaagg aggcacttac tatgattact tacaaaaata ttttaatcgc
     2701 agttgacggt tcacatgaag cggaatgggc atttaacaga gcagttggtg ttgctaaacg
     2761 taacgatgcg aagttaacaa ttgtgaatgt aattgattca agaacatatt cttcatatga
     2821 agtttatgat gctcaattta ctgaaaaatc taagcatttt gcagaagaat tattaaatgg
     2881 ttataaagaa gtagctacta acgctggtgt taaagatgta gaaacgcgtc tagagtttgg
     2941 ctctcctaaa tctatcattc ctaaaaagct tgcacatgaa attaatgcag acttgattat
     3001 gagtggtaca tcaggcttaa atgccgtgga aagatttatt gttggttctg tatcagaatc
     3061 tatcgttcgt catgcaccat gtgacgtgtt agttgttcgt actgaagagt taccagcaga
     3121 cttccaacca caagttgcaa caactcaatt acgtgaaaaa tatcaaaatt aaatatagat
     3181 gatcattaga taactacttg atcattatga accaagtgca actgtctgta cttggttttt
     3241 tattgtttat aaataaaatt catttaataa tgttttcata atcttcttcg actacttaat
     3301 tttttaagat attcgtgaaa agagacatta cactagttaa ttttcaaact atacaaaaag
     3361 cgtctacctc ctacatataa ttgtagcgga gatagacgct taatatttat taaaaaatta
     3421 ttttaaacca ccgaatgtca taacatcacg ggcaatcatg ctttcttcat ctgttggaat
     3481 aacgacaact ttaactggtg aatgaggata gttaataaat ccttctttac cacgtagtaa
     3541 gttttcattt ttcttaggat cccaataaac acccataaat tctaagcctt caagaacttt
     3601 cgcacgaatt tctactgagt tttcaccgat acctgctgta aatacgataa catcaacacc
     3661 atgcattctc gcagcatatg atccaatata tttgtgaatt ttagaagcaa atacatctaa
     3721 agccatttgt gaacgtgctt tacctgattc agcttcttct gataagtcac gtaaatcact
     3781 agatgtacct gataatccta ataaacctga ctctttgttt aagatttcca atacttgttc
     3841 agcagtttta ccagtttttt ccataataaa tggaattaaa gcagggtcaa tattaccaga
     3901 acgagtaccc attgttacac cagcaagtgg tgtgaagccc attgatgtat caatagattt
     3961 accgccatcg atagctgcaa ttgatgctcc attaccaatg tgacatgaaa taatacgtaa
     4021 atcttcaatt ggcttatcta acatttctgc cgctctttgt gatacaaatt tatggcttgt
     4081 accatggaaa ccatacttac gaatgccata atctttataa taatgatatg gcaagctata
     4141 tagatatgct ttttcaggca ttgtttgatg gaatgctgta tcaaaaattg ccacatgagg
     4201 gatatttggt aataatttac ggaaagcacg aatacccatc aagttagctg ggttgtgaag
     4261 cggtgctaat tcgcttaatt cttcaatttc cttttcaacc tcatcagtaa tagctactga
     4321 ttcagggaat ttttcaccac catgtacaac acggtgacct gttccatcga tatcgttaat
     4381 atcattaata atattgtgcg ctttaaaagc atccaacatg atatcaactg cctcaacgtg
     4441 atccttgata tcttgtactg ttttaacttt ttccccgttg acttcaattg taaaaattga
     4501 atccttcaat ccgattcttt ctactaaacc ttttgttact aattcctctt caggcattct
     4561 aattaattga aattttaatg atgaactacc agcattgata gccaagatta attttgacat
     4621 aagtaatgat cctccatcgt ttgcttttta tactatttca tttctcattt tatcattcat
     4681 acagttttat gactatacta ttacagatta ttttttagga cgatttgtgt ccatccactg
     4741 atttaactct gtcataaatc cttgaaattg tgaaggattt ttgaaatcag gaatatttgc
     4801 caataatact tcaactggct ttgtttcacc cgattttttc ttttgtaaaa ttaatataga
     4861 ttttcgcgct ttttcatttt taaataaagt tggtggtaaa tttaaaaatg cttgcatctc
     4921 tgtctctgtt gcaatatatt tttcaagctg ttttacatgt tcgcctgtaa aaatattact
     4981 cggtaccact agaaaggcat atccagcatc ttttaatgca tttattgctt gttctattaa
     5041 taaataatgt gaataactat gtccttcttc gaaacctagc ttaaactcct tacttctttc
     5101 atcaattgga taatagccta ctggaaaatc accaataacg atatctgctt cttctaatgg
     5161 tagtggcata atggcatctt gaggatacac atcgaaagga atttctaaga agtttgctaa
     5221 atgtacacta acacgtgata aaactggatc aacttcaatt aaatgatgca taaccgcaat
     5281 ttcaggtaac acttctttta cagtagcact taaatgacca gcaccacttg caatatcaac
     5341 aatatgtaat tcttcttggt tgttcataaa acgctcaact aaaaatccta gtatcaagcc
     5401 aattgaatct ggtgtaattt gatgatttgc ttgtatcttt tcttcctgca ttaaacttaa
     5461 atatgcaaat tggaatgctt tacgtcgatc ttgtaacgtc gattgttcta acaatcctct
     5521 ttcattggta tatacttgtt ccattgctag tccaagattt tcaataaaac tttggccatt
     5581 ttcattattt aatgttttag ctttttcatc taatgtatga aacaagcgtt ccataattgt
     5641 ttgttgttct gccatattcg tccctctcca ataaaaagtg cccataggtc ttagcaatca
     5701 tagaaaacaa ttctatttta atgctgttga caacgatgga cacgatgtat acattttaag
     5761 atttatctct ttaatgatta aatatttttg tatgcagtta aagcagcatc aaaatctggg
     5821 aaatcggtac cttcactaac gatttcttta taaacaactt tattatctgc atctaataca
     5881 aatactgcac gagctaataa gcgaagttct tccataacaa cgccatagtt ttcaccaaat
     5941 gataagtcac ggtggtcact taatgtaatg acattgtcta aacctgcaga cgcgcaccat
     6001 cttttttgtg cgaatggtaa gtctgctgag attgtaagca caatcccctc ttctttagaa
     6061 gcttcagagt tgaatttgcg agtctgctga tcacaaacac ctgtatcaat tgatggtacc
     6121 acactaatta atttcttttt accagcataa tctgctaatg ttacttgatt taagtcatta
     6181 tctaacactg taaaatcagg tgcaaaatca ccttcattaa tttgttgacc ttttaagtgg
     6241 attggtccac ctttgaatgt tatttcagtc atatacttga cctcctgcat tttaaaattc
     6301 taactacatc atacgacaat ttatggtttg attacaattt tcatgcttta atcaaaagca
     6361 aatttaattt tatacaaatt gttgaatata atcaaaagca ttttttaaaa ttaaaatcgc
     6421 agtaataata ataaataata cttttacata gccaacacct tgtttgatag caaaatgagc
     6481 cccagtatat gacccagcaa tcatgcttgt agccataatt aaacctatta cataatctac
     6541 ttgtcctaat accataaata atacaagcgc acctatatta gaagcaaagt tcaaaacttt
     6601 agcatttcct gcagcactta aaaaatcaaa accaaagact aacaatacaa aaagcataaa
     6661 tgaacctgtt cccccaccta caaatccatc ataaaagccg attaatataa aaagtgctgc
     6721 aaatagtatg gctttcttaa atgtaaattg agtaaacgta cgtgtattgc cccaatcttt
     6781 tttaagtaat gtgaatataa acaccgacga aagtgcaata atgattaaag gtttcaatat
     6841 ttgtgacgga accatcgttg caatatatgc tccacatgca gatgccaaaa atacaaaacc
     6901 aaataatttg gcaacaacat ataagtccac tttaccggac cttataaact ttatcgtact
     6961 agttaaagaa ccaaatgaac ttgccaattt atttgtacct aaagccacag atggtggtag
     7021 accgattgct aataatgctg gcgtagaaat taaaccgcca ccccctacaa ccgaatctat
     7081 aaacgccgcg ataaaaccaa ataaaattat gattataatc atcgttaagt tcaaatccat
     7141 agtttaacat ccccttatgt ttaaatttac tctaggattt tatttataaa aagtcgttta
     7201 ttaattggtt tgtttgctgt tctttaatag ttttataatc actagttatt tcaagtgttt
     7261 caatattttc aacagcacga ttaatcatct cttcaaaatc aaagacactt tcatattgaa
     7321 ctaccttatc aaagtttggt tcggttactg gatttttagg ggtgaaaatt gtacaacaat
     7381 cttcaaatgg ttgaatagat gtttcaaatg taccaatttc tttcgattta ataataattt
     7441 cttctttatc gtaagttaat aaaggacgta atacaggggt agacgtcaca ttattaattg
     7501 catacatgct atgaagtgtt tgactggcta cctgccctag gttttcacca tttacaatag
     7561 ctaaagcccc tatttgatgt actaatttat cagcaacacg catcatcata cgtctcgttg
     7621 aagtcattgt atatcttgga tgtacaactt tatttacctg tttttgcaat tctgtaaatg
     7681 gtacaatatg caatttaatt ggtccaacac gttcagctaa aatacgtgtc aattcaataa
     7741 ctttttcttt tgcttgatca cttgtaaatg gtggactatg gaaatgaatc gcttcaattg
     7801 ttacgccacg tctcatcact tccatcccag caactggtga gtctatgcct cctgaaagca
     7861 ttagcaacgt tttaccacct gtacctactg gtaaaccacc agcaccagct accacttctt
     7921 catacatata aattgcatct aatctaactt ctactctaat ttcatgatct ggtcgtttaa
     7981 cattcactga aatattgtcg aagtgcttca atactgcacc acccaattca cgttgtaatt
     8041 catacgtatc cattgggaaa tttttatcgg cacgcttcac atcaatttta aatgtgctgt
     8101 tttcttcaaa ttgctgcgca aatttaattg ccgctgcact cattgcctct aatgtttttt
     8161 ctacttttaa tactggacta atagatttaa taccgaaaat ttttgataat cgatatgtta
     8221 tttcatttat atctgcatgg tcttcaagtt caatatacat acgatctcgt ttgcctttaa
     8281 cgacaaaccc atcaagtcct tttaatgact tatttacatt atttcttaat tgatttacaa
     8341 atttctttct atttgaaccc tttaatgtta actccccgta tctaacaagc aagtgatcat
     8401 acttcattat tttagcaact ccttaatttc ctcataaatg atgataaata tttctttaaa
     8461 ccttgctata tcttctttag ttgtagtagc cccaaatgac agtcgaatac taccttcaat
     8521 agacttgtct gataatccca ttgcagccaa tacttcattt aatttattac gtttagatga
     8581 acaagcactc gtcgtagata tcataatgtc atattttgaa aaagcattaa ctaatacttc
     8641 accttttacg ccaggaaaac taatatttaa aacgaatggt gaacctgaag cggatgaatt
     8701 aatataaaca ccttgatatt tatttaaaaa ttgacggacg tcattattta actcagtaac
     8761 aaatgcattc aatgcttcaa ggttttcatt agctatcttc atcgctttaa ccattgcaat
     8821 atcatttggc aaattaactg ttccacttct aacgccatat tcttgaccac caccatggac
     8881 agttggttca acattttgaa tgtgatttac aagtaagacg ccttgacctt ttaaaccatt
     8941 aaacttgtgt ccacttaaac tcacgctatc tacatttgtt aaatccattg aaattttgcc
     9001 gaatgcttga accacatcta catgaaaatg tgccttagga taatttttta taacttcagc
     9061 catttgtgga ataggctgta tttgtcctgt tacattattt acatacatac atgttactaa
     9121 accgactttg tctgacatta attctttgaa gtgttctaag ttaatactgc catctttctt
     9181 tacatcaaca tatttaactt taaatccttc gtgcgcttcc aaatatctta caacctctaa
     9241 tacggatgga tgttctaaca cggatgtaat tatttcctta gctgtatcaa atttacgata
     9301 agacacacct ttaagcgcga tattgttaga ttccgttgca ccactagtga atacaacatc
     9361 ataatttgtt tttgaattaa tcattgcatt aatttgagct tttgcttgtt gtagtaattg
     9421 atttgcctgc aaaccagctt tatgcggact attcggatta taatatattg attgatttac
     9481 ttttaaataa gtatctaaca cttcttcaaa tgctttcgtc gttgccgcat tatctagata
     9541 tatcaaaata aacacttctc tttcattaat catcaatcgt taaaattgtt ttactcaagc
     9601 attatactat tatccgacaa aaaataaagg ttatagcgca aaataatctt atttctactt
     9661 attaaataag aattagacta tttcactcta tgcatacagc tgtgtgatta tacttttttc
     9721 actacttata ttaatttaaa caacacattt gattacgttt gtgtttaaac aattgctatt
     9781 tatatttatt gtccctttca tatatccata taaaaaagaa gctagacaac atacagttgg
     9841 ctaactccta tcacttcgta aaatacgatt aagtttaata aatgctattg cttaataact
     9901 tcttcttcaa tatgtttagt gacacctggc tcaacacttt caagagcttg ctctgcaatt
     9961 tcaatcgcac gcttatagcg attattttta aataatcgct cagcttcatt taagctctta
    10021 tcaacattgc tatagtcctt acgatatcta tttccatatt gaattaattt ctctgcataa
    10081 acagcattaa caagaacatc atttgcttca tcttcaaatg tattcatttg aatcacaatt
    10141 ttagacactt tatcttttaa ctgtttaacg tgtattggac gttcactaaa ttgttcgtta
    10201 acatcacgaa cttcatgatc aatttcattt ttcatgatga taaacctttc aggaacgctt
    10261 gttaagttag aagcaagtaa tcgacgatac acttcttctt tcttcgattg tactcgtagc
    10321 agattgtctt ctgcttctgc ttcatcttca cgcaattgaa tcagatgatt ttgtagcttt
    10381 tcttgtttgt cattaataac tgtgacatga tcttctaaat attgtaaatt atcctgaacc
    10441 tcgctatatc gtacagcaga tttagacatt tcttttaaaa tatcatcata tacagaaatt
    10501 aaactttgaa tttcattttc aaattgacga acactctgag catcagattc atttatatag
    10561 tagttttcac gtacatattc aatttctgtt tgcaatgtat aattcatgtc tttagcttta
    10621 aataagttat ccgtaatgat atcttttgtt tcttcgacat catttttagc tttaacttca
    10681 tgttcaatta aatcatacat gtcatctaac ttatcattga tattagctag tttatcatta
    10741 gcttcttcta attctaagcg gctaattaat ggttcaacga aactaagctc tgtttttaag
    10801 ctttgtaatg tactgtctac ttttacgtga tccagatcat acccttcaac tttaagatca
    10861 cggcaaccat attttaaatc ttgaaattga ccaggtaatt ctttttgagt ttctctaatt
    10921 aattctggta tttcttccat ataagatctt agctgtttca tttgttcatt caaggcagct
    10981 atatggttgt gcgcttgtac ataattacca tcagctttta gtacttcata ttgctctaac
    11041 cttggctcga atttttcaat ttcagtttca agaagacttg ctgcctcacc aaattgatga
    11101 cgatttgcta aaacatcacg tttcatttca cgataatcaa ccttacattt gtcatataat
    11161 tcatcattat ctttgtataa cgcaataatt tcatttacat cttctaattg ttgctgatag
    11221 ctttgttcgt aactatccat caactcattt gcatcatcaa tttcactttg agatgcgttg
    11281 aaactaaatt tatctaataa agcctcagca ttatggattt tttcttccac aggagctaga
    11341 tacttatttg tactttctac gttgtccttt ttcattgcat cgtatttcgt ttttgtttca
    11401 ccttttaaat tcaacttaga taattgtgca aggttttgat caaaaggtaa cgtctcaatt
    11461 tcatttttac gttcgattgc tttttcaata atttgtcgtt tatttgaacg taaatagaat
    11521 aatacaccta cagcaatcaa tataatcaca attattgcca aaatgatata taacaccata
    11581 tgcttctcct cctaatttat cattactatt ataacgtaat tttgcttaag gataaattat
    11641 aaaatgaatt tattgccata atattttcga aattctatac tattataaaa tctatgaaaa
    11701 taagaggttt taaatatgac aacaattaac ccaacaaact acacattatt aaagaaacaa
    11761 gcagcaagcc ttattgaaga tgaacatcac atgattgcta ttttaagtaa catgtctgca
    11821 ttattaaatg ataatctaga tcaagttaat tgggtcggct tttacttatt ggaacaaaat
    11881 gaacttatac ttggaccttt ccaaggacac ccagcttgtg tccacattcc aattggaaaa
    11941 ggtgtatgtg gtacagccgt ttcagaacgt cgtacacaag ttgtagctga tgtccatcaa
    12001 ttcgaaggac atatcgcttg tgatgctaat agtaagtctg aaattgtcgt tccaattttc
    12061 aaagatgata aaattatcgg tgtcttagat atcgatgccc ctataacgga tcgatttgat
    12121 gacaatgaca aggaacatct tgaagcaatt gttaaaatta ttgaaaagca actcgcataa
    12181 aaggacatca gcattttcaa taaagtgttg acagttagca ggaaaatgtt acaataaact
    12241 ttgtgtgaat taacgaaagt agcagttgta tattattgag cgctatgttg ttcccaatgc
    12301 ggacgtgtca tgtaactgtc gctataaggt gaagacacat aaaacaatat atcttagtaa
    12361 gcatgcaaca ctcttttttg tttattcata acaacaaaaa agaattaaag gaggagtctt
    12421 attatggctc gattcagagg ttcaaactgg aaaaaatctc gtcgtttagg tatctcttta
    12481 agcggtactg gtaaagaatt agaaaaacgt ccttacgcac caggacaaca tggtccaaac
    12541 caacgtaaaa aattatcaga atatggttta caattacgtg aaaaacaaaa attacgttac
    12601 ttatatggaa tgactgaaag acaattccgt aacacatttg acatcgctgg taaaaaattc
    12661 ggtgtacacg gtgaaaactt catgatctta ttagcaagtc gtttagacgc tgttgtttat
    12721 tcattaggtt tagctcgtac tcgtcgtcaa gcacgtcaat tagttaacca cggtcatatc
    12781 ttagtagatg gtaaacgtgt tgatattcca tcttattctg ttaaacctgg tcaaacaatt
    12841 tcagttcgtg aaaaatctca aaaattaaac atcatcgttg aatcagttga aatcaacaat
    12901 ttcgtacctg agtacttaaa ctttgatgct gacagcttaa ctggtacttt cgtacgttta
    12961 ccagaacgta gcgaattacc tgctgaaatt aacgaacaat taatcgttga gtactactca
    13021 agataatacc gttcaataca aacacccaca attgtgggtg tttttttatt aatttaaaaa
    13081 tgatagattc aagattatat ggtgctaccc tattttacaa tcaatttaga atttacaagt
    13141 cttatttgtt gaaaaatcta tagtatttca tcatttcttt tttaaaatat caattgcgat
    13201 atacgaatcc tcatccctct atcaaataac cattcatcgt ctcacttaat aaataatttc
    13261 ggattatctg taattaatcc atctacaccc atttgtctta atttttctcc agctttcaat
    13321 ttattaactg tgtatggcat aacctgtaat tgatgatgat gtgcttcatc aacaaatttt
    13381 ctggtcacta atgcatagtt aggattaaca taactagcaa tttgagcaat tcttgaaaag
    13441 tttggctttt tataccaata attacgttta ctacaaagca cacctaattc atatatactg
    13501 cccaatgtgt ttaacttttc gatgcattcg atatcaaaag attgtataac tacttgtgtc
    13561 gcatcgactt tcttttcttc caaaaatgcc agcaatttac attctattcc tggatataaa
    13621 ttcggacttt tcaattcgat caatagcttt ttatcaaact ttaagcataa tgaaagtact
    13681 tcatctaacg tgggtattcg ttctccctta aaagcaacat ctttataact accaaaatca
    13741 aatgatttta actgcgataa tgtgtaatca gcaatacgcc ccttaccatc cgatgttcta
    13801 tcaattgttt catcatgtat cacaacaaaa tgttggtctt tggtcaaatg aacatctatt
    13861 tctaacatat caatatctag ttccatggcc tgtcgataac tagacattgt attttcagga
    13921 aatgcactcg gcaatccacg gtgcgaaaca atttgtaatt catctttcag tttattcaga
    13981 gtcattgata agtcaccttt ctgttaaact ttgataaact attctaaaat taaactatca
    14041 caattatatt ggaggttaaa gcaattgcat caacatgact ttaaagtcca aacttcttgg
    14101 caaggtggtc gtaacaatgt cggaaacgtt caaggcgaca tactttcaga gaatatttca
    14161 atacctgctt ctttaggtgg tgttggtata ggaacaaatc ccgatgaaat gttagtatca
    14221 gccgcttcat catgttatat catctcatta gcagctactc ttgaacgtgc aaagttcaca
    14281 gatatttcaa ttgaacaaca atcgattgga acagcttgtt taaataatgg aaaattcagt
    14341 atgtcaaaaa ttgtgcacca tcctcacatt gaaattccaa gtgatcaaat agcacaatta
    14401 gaaaagcgat taccaaaatt gataacaatt gcagataata attgcatgat ttcaaatgct
    14461 gtaagaaata atgtggacat aaaaatctat cccatcattc aggccaaata aaatcgttaa
    14521 gtgataacat ttgttcgaca atatagtgtt atcactattt ttaggctttt aaattttcag
    14581 aaaatttgct aaaatagtga aatcttattt ataggaggca tataaatgta ttatcatcaa
    14641 ccgttgttat taacacctgg cccaacccct gtacctgatg ccattatgag agaaattcaa
    14701 gcacctatgg ttggtcatcg ttctaaagat tttgaagaca tcgcacaaca agcatttcaa
    14761 ggtctaaagc caatatttgg gagtcaaaat gatgtactta ttttaacatc tagcggtaca
    14821 agcgtcttag aagctagtat gttgaacatt gtaaaccctg aagatcactt cgttgtcatt
    14881 gtttcaggtg cctttggtaa ccgatttaaa caaattgcac aaacttatta caaaaatgtg
    14941 catatttatg acgtaacatg gggagaagct gtagatgtca aagatttcat caattacctt
    15001 tcaactttaa atgttgaagt taaagcagta tttagtcaat attgcgaaac atctacgaca
    15061 gtgctacacc ctattcacga gttaggaaat gccatcaatc aatttaatag taatatttat
    15121 tttgtagttg acggcgtaag ttgcattggt gctgttgatg ttgacattaa caaagataaa
    15181 attgatgtac ttgtttctgg tagtcaaaaa gcaattatgt tacctccagg attagctttt
    15241 gtagcttata gccaccgtgc aaaagaacgt ttcaaagaag taactacacc aaaattttat
    15301 ctagacttaa ataaatacat ttcgtcacaa gctgacaatt ctacaccgtt cacaccaaat
    15361 gtgtctttat ttagaggtgt aaatgcatac gttgaaaccg taaaagcaga aggtttcaat
    15421 cacgtaatag cacgacacta cgcaattaga aatgcattaa gaagcgcatt aaaagcatta
    15481 gatttaactt tattagtcaa tgataaagat gcatctccaa cggttacggc attcaaacct
    15541 aacacaaatg atgaagtgaa aataatcaaa gatgaactta aaaatcgctt taaaataaca
    15601 attgctggtg gtcaaggcca tcttaaaggt caaattttaa gaattggtca tatggggaaa
    15661 attagtcctt tcgatatttt atcggtagta tctgctttag aaattatttt aactgaacac
    15721 cgtaaagtta actatatcgg taaaggtata tcaaaatata tggaggttat tcatgaagca
    15781 atttaatgta ctcgttgcag atcccatatc aaaagatggt atcaaagcat tattagatca
    15841 cgaacaattc aatgtagata ttcaaactgg cttgtccgaa gaagcattaa tcaaaattat
    15901 accttcatac catgctttaa tcgttcgtag tcaaactacg gttactgaaa atatcataaa
    15961 tgctgctgat tctttaaaag taatcgcacg cgccggtgtt ggtgtagata atattaatat
    16021 tgatgctgca acattaaaag gtattttagt tattaatgcc ccagatggta atacgatttc
    16081 agctactgaa cattcactag caatgttatt atcaatggct cgaaatattc cgcaagcaca
    16141 ccaatcactt acaaataaag aatggaatcg aaatgcattt aaaggtactg agctttatca
    16201 taaaacatta ggtgtcattg gtgctggtag aattggttta ggtgttgcta aacgtgcgca
    16261 aagtttcgga atgaaaatac tagcttttga cccttactta acggatgaaa aagcaaaatc
    16321 tttaagcatt acgaaggcaa cagttgatga gattgcccaa cattctgatt tcgttacatt
    16381 gcatacacca ctaacaccta aaacaaaagg cttaattaat gctgactttt ttgccaaagc
    16441 aaaacctagt ttgcaaataa tcaatgtggc acgtggtggt attattgatg aaaaggcgct
    16501 aataaaagca ttagacgaag gacaaattag tcgggcagct atcgatgtgt ttgaacatga
    16561 acctgcaact gattcgcctc ttgttgcaca tgataaaatt attgttacac ctcatttggg
    16621 tgcttcaaca gtcgaagctc aagaaaaagt ggcaatttct gtttcaaatg aaatcatcga
    16681 aattttaatt gatggtactg taacgcatgc agtgaatgca cctaaaatgg acttaagcaa
    16741 tatagatgat actgtaaaat cattcatcaa tttaagccaa acagttggtg aattagctat
    16801 tcaattaatg tacaatgcac caagctctat taaaattacg tacggtggcg acttagcctc
    16861 tatcgatagt agtttattaa cacgtacaat tattactcat attttaaaag atgatcttgg
    16921 tcctgaagtc aatattatca atgctctaat gttgttaaat caacaacaag tgacattaaa
    16981 tattgaaaat aataaagcag agacaggttt tagtaactac ttagaggtag aactatcaaa
    17041 cgatagcgat tccgttaaag ttggcgcttc tgtctttaca ggtttcggtc caagaattgt
    17101 tagaattaat aatttttctg tagacttaaa gccaaatcaa tatcaaattg tgtcatatca
    17161 taatgatact ccaggtatgg taggcaaaac tggcgcattg ttaggtaaat acaatatcaa
    17221 cattgcatct atgactttag gtagaactga agcgggcgga gatgcgctaa tgattttatc
    17281 cgttgatcaa cctgtttcaa acaatataat tgatgaactt aaacaagttg gtgaatacaa
    17341 tcaaattttc acaactgaat tgacggtaca gtcataaaca atttatgtaa attcaaaatg
    17401 ccaagaagac attggattac ttccattgct tcttggcatt ttttcattat taatttcatt
    17461 gtaactcaat atgaaacctt tgtttcaatc tttcacttca agttatgctg tcaaacaaca
    17521 atcaaattac aaattatcta gtacacctct aagttcacct aaatgattaa taacatagtc
    17581 ggcatgatgc gcttctaact cacctgcagc atccttacct tttaaacctg ttaatgttcc
    17641 aataaacgtt gcacctattt tttgagcact taataagtct gctaacgaat cgcctactat
    17701 aaatacgtca tctttattta caatgttatc ttgcttattg atataagatt catatttatc
    17761 gcgattatta ccatataaag ctgcgatata actaaaagga ttcggctttc ctaatggtcg
    17821 tgcttgcgga tacattttct ctgcttctaa aacatcactt gctgttgcaa taaaatcagc
    17881 ttcaaaatat ggtcctaaat tttcaaatgg cacaacagtc tcagtataag gacgacctgt
    17941 tgcaatacct aattcgaaac cagcaccttt taaatcattc agaagtacct taacttcatc
    18001 tactggtctc aaaataattt cttgataaat ataacctgtc ttaaaagtag ttcgtgctat
    18061 tttcttttca acctcttcat acaacttcga tcctaaatac cattcttgat aaacttcttg
    18121 cgctaacgtc cataatgcac ccttcaaact aaataaagta gcatccgaaa catgtaactc
    18181 tgttattgca aactcttcca aagcagcata aatattattt ttaccaactt ttacattatc
    18241 caaaaattgt aaaggtagtt gttcatttaa attaaaacag tctgctaagt ttgtgcttat
    18301 attttgccac ttcaattcga caggctcatc ttgatacatg aatgcctcaa tttcatcatg
    18361 tgataacttt ttcaaaatat caattaaatg aatactaaaa acgataaata acatatccca
    18421 atttgaattc agccctagcg attttaattt atttaaaatc ttatcttttt gaaaaattcg
    18481 attcctaatg tcttgtatat cgttatcagt caaagtttcc caatctatat gtgaatgaag
    18541 acctaaataa cacttatcca ttaataattc atataccgtt aatgcagaga catcgaaaca
    18601 acgttcttcg cttaaaaaaa cgccatcaac atcaaataaa attttcttca caatccccac
    18661 tccatttctg aaaattcaga tataaatcat tctactattt gactaaaaaa agcgcaaacc
    18721 ctattgaagt tagatttgcg ctttagctgt ttgaatttta taaatgtttt tcaatttcat
    18781 cagcaacctg ctgtacgtgt gtaccgacaa taacttgagt tgaatgtttt ccattaacag
    18841 taacaccaac tgcaccggcg tttttaatct tctgttgatc aataatagat gtgtctttta
    18901 actctagacg caaccttgtt gcacaattgg ttaaattaac aatattctct tgaccaccta
    18961 aaccttctaa tatttgtata gcatgattat gatatttact ttgtttaata tcattttcac
    19021 caggagcaat attatctttt acaactgttg gatcaactaa ttcattttca cctctaccaa
    19081 tcgtatttaa gttaaatact tggattacta cacggaaaat cacatagtat aagatgaaga
    19141 atacaatacc ttgaacaagc aacatcaatg gatgatttga taccggatta attagtgata
    19201 acacataatc tatcaaacct gcactaaatg aaaatccagc tgtccaatgg aatgtagctg
    19261 cgataaataa agataatcct gttaataacg catgaacaac atataagatt ggcgcaacaa
    19321 acataaatgc aaattcaatc ggctctgtaa caccaacgaa aaatgctgca actgaactcg
    19381 ctaggaacca accgtaaact tgttttttct gagtagtttt agctgtatga tacattgcta
    19441 acgcagccgc tggaataccg aacatcatga ttgggaagaa tcccgcttga tagcgtcctg
    19501 taataccttt tatagcatct ttgccacttt ggaatttacc aatatcatta ataccaatcg
    19561 tatcaaacca gaacacacta ttcagtgcat gatgtaatcc tgtaggaatt aataatctgt
    19621 tggcaacacc atatacgaaa gcaccaaatg atcctaaacc aactatagat tcaccaaatt
    19681 ttacaatcca tgaataaagt agtggccata agaataacaa tatgacaact aaaaatgtac
    19741 agtaaaatgc agtcataatt ggaactaaac gtttaccact aaaaaatgat aatgctaatg
    19801 gtaattctgt ttcactaaac ttattgtatg cataagctgc tattaaacct attacaatac
    19861 ctacaaagac attgccatta ttcatctttt caaaagctga atttatttcc gaagctttca
    19921 ttcctaataa aggcgctaat ttcattggtg ataatacaac tgtaactaaa aaatatccta
    19981 acgcagctgc aagcgcgact gcaccatcat ttttctttgc cattcctata gctacaccaa
    20041 ttgcaaataa aatacctaat tgctctaaaa tcgtagtacc taccgtagta aagaacattg
    20101 cgattttcgg cgccgcatga agtgcattta acgtattacc aattccggca ataattgctg
    20161 cagccggtaa aatggcaact ggtaacatta acgaacgccc taaattttgg aaaaatttat
    20221 acattgaatg tcatccttct taaaataagg tagaaatata aagattacta atgtaactag
    20281 aataactact tcgatactcc gttatagtca cctaggctta ctaaccagct atatttctac
    20341 ctcaagttat tttataaact ttttacaatt tcatgcaatt cttgttgtaa ctttgctgtt
    20401 cgtgtttcaa tctcttttgt aatgtaatcg atacgctcgt ttcgttttaa atctttaggt
    20461 aaatcgttaa tatcgattgg tttaccaata tttatgtatg cttgtcctgt taaaagacca
    20521 tgaatcttag taggaccaac ataagcaaca ggtaatattg gtgacttact taacattgca
    20581 attgttgaag caccacgttt caaaggtgca ccttcttgcg atgtgcgaga acctgttggg
    20641 aagataccaa ctgtcttatt atctttcaac aaattgattg ggcgttttaa agtactaggt
    20701 cctggatttt cacgatctac aggaaatgca tttaaagacg ttaaaaattt accaatccat
    20761 ttatttttga acaattcttt tttagccata taatgaattt gattaggata taatgccata
    20821 cctagcataa tgacttcgtt ataactttca tgcgtacaag ttacgacata tttactatcc
    20881 ttaggaatat tatctttacc gattacgtat aatgattttg acattttaac taaaatgaaa
    20941 ttcaaaatct tactaatcac tgaatacatt gtgccaccta cttaacttat ttgtttataa
    21001 caactagatt ttatcactat atttttacta gaacaagata gtctgaaaat acatctaaag
    21061 ttgtatagtg tattactagc aataattata aaaaaaagac ataattaaag catatataaa
    21121 tcatataatg gaggttaagt atgtcagatt ttaatcatac agatcattct acaacaaacc
    21181 atagccaaac acctagatac agaagaccta aatttccatg gtttaaaaca gtcatcgttg
    21241 cattgattgc tggaatcatt ggtgcacttc tagtacttgg tataggcaaa gtattaaata
    21301 gtacaatttt aaataaagat ggttcaactg tccagacaac aaataataaa ggtggcaatc
    21361 aattagacgg tcaaagcaag aaattcggta ccgttcatga aatgataaaa tctgtctccc
    21421 ctacaattgt tggagttatt aacatgcaaa aagcatcaag tgtagacgac ttattaaaag
    21481 gaaaatcatc taaaccatct gaagccggag taggttcagg tgtcatctat caaataaaca
    21541 acaattcagc ttatatcgtt acaaacaatc atgttattga tggcgcaaat gaaattagag
    21601 tccaattaca taataaaaaa caagttaaag cgaaattagt tggtaaggat gcagtaactg
    21661 atattgctgt acttaaaatt gaaaatacaa aaggtattaa agcgattcaa ttcgccaact
    21721 cttcaaaagt acaaactggt gatagcgtat tcgcaatggg taacccatta ggattacaat
    21781 ttgctaactc tgtaacatct ggtatcattt cagcaagtga acgtactatt gacgctgaga
    21841 caactggtgg caatacaaaa gtcagcgttc ttcaaacaga tgctgctatt aaccctggta
    21901 actcaggtgg cgcattagta gatattaatg gtaatttagt tggtattaac tcaatgaaaa
    21961 ttgctgcgac acaagttgaa ggtattgggt ttgccattcc aagtaatgaa gttaaagtaa
    22021 caattgaaca acttgtaaaa catggtaaaa tagaccgccc ttcaatcggt attggattaa
    22081 ttaatttgaa agatattcct gaagaagagc gcgagcaact tcatactgat agagaagacg
    22141 gtatttatgt cgcaaaagct gatagtgata ttgatcttaa aaaaggtgat attattacag
    22201 aaattgatgg caagaaaatt aaagatgatg ttgatttaag aagctattta tatgaaaata
    22261 aaaagcctgg tgaatcagtc actgttaccg ttatccgtga tggtaaaaca aaagaagtta
    22321 aagtgaaatt aaaacaacaa aaagaacaac caaaacgtca aagccgatca gaacgccaat
    22381 cacctggcca aggcgataga gatttcttta gataatttac agacagaaaa gtacattaag
    22441 agcctactca tattcattcc taagaatgta atgagtaggc tctaatttat ttagctatgc
    22501 aattatattt attgatagtt aaccatgaag tattttttct taccgcgacg aataatcgta
    22561 aattcgccat caattttatc ttctggtgct aaagcataat taacatcttg ttgtctctca
    22621 ccattaatat aaatcgcacc attgttaaca tcttcacgtg cttgtcgttt agaaggagaa
    22681 atgcctgttt caataaggac ttcaacgata tttgttgtgt catttgataa tgtcacttga
    22741 ggcacatctt taaatccatc ttttaattct ttcgctgata atgattttaa atcaccacta
    22801 aataatgctt gtgaaatacg gattgcatca tttaatgcat cttcaccatg aataaattta
    22861 gttacttctt cagctaatgt tttttgagct tcacgtaaat gcggtgcttc atttttagat
    22921 tgttctaagc gatcaatttc ttcttttcct aagaaagtaa agtattttaa gaatttaatt
    22981 acatcttcgt ctgattgatt aatccagaat tgataaaatt cataaggact tgttttttca
    23041 gcatctaacc aaacagcacc tgactcagat ttaccaaatt tcttaccatc tgatttagtt
    23101 acaagcggaa tagttaaacc gtacgcgtct gtttgaccat acatacgacg cattaattca
    23161 ataccacttg tgatattacc ccattgatct gatcccccta cttgaatctt acaattcaat
    23221 tctctattca aatgaccgaa atcaatagct tgtaaaatcg tgtatgtgaa ttctgtatat
    23281 gaaataccat gttctaaacg actttggatt gaatctttac ctaacatgta attaacgccg
    23341 acgtgtttac catagtcacg taaaaagcta attaatgaaa tttgtcctaa ccagtctcta
    23401 ttattaacaa gcactgcacc atggtctgtt ccaaattcaa aaatattgtg catttgctta
    23461 ctaatacctt cgatattttt atctacttgt tcttctgttt gtagcacacg ttcttctgat
    23521 ttacccgatg gatcaccaat catacctgta ccaccgccaa ttaaaacgat aggacgatgt
    23581 ccatgttctt gaaaacgtct taatgttaag aatggtagta agtgaccaat gtgtaagcta
    23641 tctgccgttg gatcggcacc gcagtataac gtcacttgtt ctttatttaa taaatcttca
    23701 ataccttgtt catcagtttg ttgataaata agacctctcc attttaaatc ttcaattaat
    23761 acattcgtca ttattatttc ctcctattta taattattta ttgaatactt gttaaaatac
    23821 tttaaagctt tttgaacgta aaaaaaccct tacaacaaat atgtaagggc gcgattgcac
    23881 gttaccacca aacttaaaca taatcataag ataatgttca ctctattaat gatacgttca
    23941 ttaataaacg taggacatgt tagttataaa ggtgtattca tattattaaa aacactagtt
    24001 cacagcgacc actagctctc tgatgatttc aaataatatt acttgtcctt ttatcctatt
    24061 ctttatgcat tttccctaat tataattaac gttaaaataa aagtcaaatt gcttaaatat
    24121 ggtatactat acgtaattta ggaggttaaa gatgacgaat caagacaaca atcatcaatt
    24181 gaatcatcgt atatatcatt ttgaaaagat atataaagct atcaaacatg tcattgtttt
    24241 tatatttatg attttcattg ccatcgttgc tatcgctgtg attgcgatgt ctttatattt
    24301 tcatcattta actaaaacgt ccgactcatt atcagatgat gctttaataa aaaaagttcg
    24361 acaaatacct ggcgatgaat tattagatca taataacaaa aatttgttat atgagtataa
    24421 ccattctcaa aactcactca ttataggccc taaaacatca agcccaaatg tcattaaagc
    24481 attaacgtca tctgaagaca ctttatttta taaacatgat ggcatcttac caaaggcgat
    24541 tttaagagca atgatacaag atatttttaa tactgatcaa agttcaggtg gaagcacaat
    24601 tacacaacaa cttgttaaaa atcaagttct taccaacgaa aaaacatata gtagaaaagc
    24661 aaatgaactt cgcctagcaa ttagattaga acacctactc tcaaaagatg aaattatata
    24721 tacatattta aatatagttc ccttcggcag agattataat ggcgctaata tttccggaat
    24781 tgcatccgct tcatatagtt tatttggtat tccaccaaaa gatttatcaa ttgcacaatc
    24841 tgcatacctt atcggtttgt tgcaaagccc ttatggctat acaccctacg aaaaagatgg
    24901 aacgttaaaa tcagataaag atttgaaata tagtattcaa agacaacatt atgtattaaa
    24961 gcgtatgtta atcgaagatc aaatcactga aaaagaatac aacgatgcat taaaatatga
    25021 tattaaatca catttgttaa atcgaaaaaa gcgttaattg atgctcactt tttaaagtaa
    25081 ccacaacaat gcatccaaat attaaaaaca gcagtaagtt tattttcaat tagaaaattt
    25141 ctcactgctg ttctttattt taaatactct aaaggaaagc cattaaacca atcaatagtt
    25201 tataatttaa ttactttcac attgtctaca aagttaatca tacatttgct atatccagtt
    25261 aattatttag attcttttct gaatttagga atgaataaag ctaacatacc taataacgca
    25321 tataagcccc accatgattg gcttgaagtt gtttctccag tttttggtaa cattttagat
    25381 tgactaagtt tgttttcttt cgatggtgtg ccggcctttt cttttgcttt cactgttttg
    25441 tctacagttt tatgaatatc actcggcaga tgttcagttg tttttttgtc agtaactttg
    25501 atagtactga taggatgata tttcattgta tcaatgtttg tttcagttcc agtattatta
    25561 actttatttt tatcggcaat atgagcaagc tgtatgactt tgtctttatt tttattagag
    25621 cctttatcag tatcgacatt agatgacata ccaacgctat taacaacacc cttatcagtg
    25681 tctttagcaa tttgagtatc catttcttta agatcatact tatcgaagtg agtgttaccc
    25741 gacatatcgg atgaatgatc aatatttaga tgcgcatctt tgtcaacact actatcagta
    25801 tctttaacca tgtcagactc tggttctatc acatctactt tatcagttgc ctcacttgga
    25861 gttgtttcaa tactgttatt tgtagctgta tccgttgcag taacattatc ttcttgtcct
    25921 gtttgtacat ttagcggttc actcgtgtta ttttgtgatg tgtcatcatc ttttgtcttg
    25981 atatcctgat ttataattct gacatgatat tgaccttcat aaccaatgtt tgccacaacg
    26041 actttaacaa tcgcattgta aactgctttg tcaggtatat atgggaaaat cagcgttcta
    26101 gaattatttt taggatcttt agaaacagtt gtgacacgtt taccttctac aattaaatct
    26161 ttccagtaac tgtcatcctt tgttttcatc actacatatt tttgaccatt taaagttgct
    26221 gtatagaatg gatgttcaac aaatccatcc atgactgact cactattttc ttcactttca
    26281 aaaacaacaa aatgcgcttc ttgtaaatct gttaattgat catttgtagg tgtaacattt
    26341 tcaaattccg tcactgctga tttaacttga tcagctaact ctactctagt ttgatctaat
    26401 ttctttttat attccgcctt atatttttct ggcaatttct cttgtaattt ttctaattca
    26461 taaacttgtc tttctaacgt tttagctttg tgatacggag ctaataattt ttgtaaattg
    26521 tatgtttcct catccacata gtcgtctggg ttattagtaa taggctgtgc aaagaccatt
    26581 agcgtataat catagtcttc atggatgttc tcaccatatt caatagatga cacaatttta
    26641 acttctctcg taccattaga tactgggaaa cgaatatagg cataatcttt atcagaatca
    26701 tatgatacta attcgactgg taactttttg tcaccttcat aaacttcaaa tttcttccat
    26761 gttgaagctg tctttaaacc taattcaatt attggtcctg tttttgtaaa aatgacagtt
    26821 gctggttcaa cagtactagc ataatgataa aactgtcttt cacctttatc atttttcatt
    26881 tgaaaatcaa ttggtcgcca attatcagct gtatgttctt tatcgatgat agccgggttt
    26941 ttaattgcat cttgtagtga ttcatctgct ggtggatact gttgattaac atccgactga
    27001 ttacttgact cattcgtttt atcgttagta ttaccattag aatttgtttc atgagctggt
    27061 tggcttgacg cttgatctgc attcactgac gattttggtt gtgcaggttg actcatatcg
    27121 gtcgttgcct gcggttgatt attagcattg ttggtcgttg atgtattttg attagatgta
    27181 ttcgtatttg tttgattact tgcatctgaa cttgattgat cattcgttgc tactgcatca
    27241 tttgtatctg atttcacgag tgaaggatca ttatatatag gtttagcaaa tactaattta
    27301 gtataatcat aatttgtttc agctccatca tcaatttgag tcgaagaaac aattttcagc
    27361 tcttgcgtac catctgaaac agggaatcga atataggcat ggtcttctgg aacaggacta
    27421 tacgatacaa gtctcactgg caatttttga ttgttttcgt atacttcaaa ctttttccat
    27481 gttgaagcag tgtttatatc taattcaacc tctgctttct ttttagtgta atatacatct
    27541 gctggatctt tgatactgaa aaagtgatag tactgcgttt cattgttttt atctaataat
    27601 tgaaaattaa cttgttctct tggacctatg tcatgttctt tattttctac tgcaggattt
    27661 ttaattgcat ctttaagtga ttcatccgct gcagggtaag attttgcagt gtttgctggt
    27721 tgcgtagcag gttgtgtttg atttgtatcc tttggtggct gagttgtagt tgcattatta
    27781 ttttgatttt ccgagatttt atctgaagta tttgtatttt ctgctgcttg cgcttgatgt
    27841 tgagaagtaa ttaaaaatag tgtactgaca atgaccgatg caacgcctag agttgatttt
    27901 ctaatagaat agaaagacct taattttggg tgatgtttgt tcatgttgtt aacaactcct
    27961 ataagatagt atttaattga taataattat caatatcaat tattagtgtt aattgtagcg
    28021 catcccaatt aaatattgca acctatttta ttaaaaattt ttaatatgaa tattcattgc
    28081 atcgctttcc tattcttcaa gcccactttt tatcttcgaa tacattaatt aaccaccttc
    28141 aaacattgcc gttagatttg ccaaattgaa tctaatttta gcacagcaaa aaaccgaact
    28201 gctgaaataa tgcatctcaa acagtccggt ctattaaact atttacttat ctttattaaa
    28261 caattgccat gatatattag aataacccaa ttgcatgacc atcatcagta acatccatgt
    28321 ttaatgctgc tggtttttta ggtaaaccag gcatagtcat gatcacacct gtcaatgcta
    28381 cgataaatcc tgcacctgtt ttcgcttcta attcacgaat tgtaatttca aatcctgatg
    28441 gtgcacctaa caacgtttga tcatctgaga atgaatattg tgttttcgcc atacatactg
    28501 ggtaattatc ccaaccattt tctttaaatt gttttaattg tttttgcgct ttactgctaa
    28561 acgttacttt tgaaccgcca tagatttcag tcacaatctt ttcaatcttt tgctctaatg
    28621 gtaattctaa ttcatataaa ggtttaaatg aattaggttg atcaataact tctaatactt
    28681 catttgctaa gtcaacgccg cctttaccac ctttttccca aacttcagtt aaggcaattc
    28741 gtacgttatt ttctttagcc caagatttta catattctac ttctgcatcg gtatcatgta
    28801 taaatgcatt aattgcaaca accggttcta caccgaattt tttaatatta ttaacatgac
    28861 gctctaaatt aacaattcct gcttttactg cttctacatt ttcttctttt aaattatctt
    28921 ttgctacacc accatgcatt tttaatgcac gaatcgttgc tacaactaca actgctgctg
    28981 ggtcgaatcc agcttcacgt gccttaatgt ccatgaattt ttcagcgcct aagtctgaac
    29041 caaatccagc ttccgttaca acgatatcag ctaaatcacg tgctgtttca gttgctaaaa
    29101 ttgagttaca accgtgtgcg atattcgcaa atggtccacc atgaactaat gctggtgtcc
    29161 cttcaattga ttgtactaag tttggtttaa ttgcatcttt taaaatcatt gcaagtgcac
    29221 cttgcacttt taaatctgca actgtaactg gcttgcgatc tctagtgtaa ccaatagtaa
    29281 tacgactaat tttatctttt aagtctttaa tacttctact taaacataaa atcgccataa
    29341 tttcagacgc tactgtaata ttaaagccat cttcacgtgg tacaccattt gtaggtccac
    29401 ctaacccaac gtttacatgt ctaagtgcac gatcattcat atctaataca cgtttccatt
    29461 caatacgtct ttgatcgatt cctaattcat taccttggtg aatatgatta tctataaacg
    29521 cagacaatgc attattagca gttgtaatcg catggaaatc cccattaaaa tgtaagttga
    29581 tatcttccat aggtaagact tgcgcataac caccaccagt tgcgccacct ttaataccga
    29641 aagttggtcc taaggcaggt tcacgtagcg ctaccataac gttcttcttc aactcatgaa
    29701 atgcgtcagc taaaccaaca gtaactgttg atttaccttc acctgctggt gttggactca
    29761 tcgcagttac taaaacaact ttccctttgc tatctcttgg cgtaatttta ttaatgtcga
    29821 ttttagcttt gtaatgacca taaggttcta atgcatcctc tgaaatgcct accgatgcag
    29881 caatatcctt aattggttgt agtgttgatt gattcgcaat atctaaatct gataaatgag
    29941 tcaacttttt tcgccccttt ttgtttaatt tatgcatttt aaatatgtaa gttgtagcta
    30001 agaaattttt acttacattc atataataac gaactgttaa aaacttgtcg actaaatcgg
    30061 gttagcgctt tcatttttgc aataaattca cttattaaag ttttgcatta tacaactttt
    30121 agcgcttgat actattgata actatatttt tctattattt aaaatagcca tctttcaaag
    30181 gtcatattaa aaataaaaag caatataaag atatgccatt attgttatgt catcattttc
    30241 aaatgctttc tacgaaatga tatgacacaa gttaacagct ctttatattg cttatcgtaa
    30301 agtaaacgat tatttaaaca ttaacatgaa atatgttgtc accctttatt cttcgaaaga
    30361 aatcttctat aacattcatg tcattattcc attgtactta aatcccctgc atctaaattt
    30421 aattcccaag cttttaatac acgtctcata attttacctg agcgtgtttt aggtaattta
    30481 tctttaaatt cgatttcacg aggtgctgca tgtgccgaca aaccttcttt aacaaataaa
    30541 cgaatttctt cttttaattc gtctgttggt tcatatcctt ttctcagtgc aacaaacgcc
    30601 ttaattattt caccgcgaac cggatcaggt ttaccaataa ttcctgcttc tgcaactgct
    30661 tcgtgttcaa ccaatttcga ctcaacctca aatggtccaa ctctttcacc agctgtcata
    30721 attacatcat caacacgtcc ttggaaccag aagtaaccat cttcatcttt atatgccgaa
    30781 tcaccagata cataccagtc tccaataaaa tatgacttat atttttctgg attcttccag
    30841 atacgataca tcattgatgg ccagcctttt tttatagcaa ggttgcccat tcgatttggt
    30901 ggtaattcat tacctgcatc atcgataatt gcagcttgaa taccaggtaa tggtttgccc
    30961 attgagccaa gtttgacgtc catcgttgga tagttaacaa tcatatgtcc acctgtttct
    31021 gtcatccacc aagtatctaa caccgttaaa ccgtatactt ttttcgccca ttttataact
    31081 tcaggattta aaggctcacc tactgacaga atcgaacgta acgatgacaa gtcatatttc
    31141 tcaacaatat cgtcaccagc actcattaac attcttaaag ctgttggtgc cgtataccaa
    31201 atcgtcactt taaaatcttc aatcatacta taccactgtt ctggtgaaaa gcgaccacca
    31261 gctatacaat ttgtagcgcc atttaaccat ggtgcaaaaa taccataaga tgttcctgta
    31321 acccaacctg gatctgctgt acaccaataa acatcatctt cttgtaaatc taatacatat
    31381 tttccagaaa tatagtgcac taacatcgct tgttgaacat gcaatacacc tttaggttgc
    31441 ccagtagaac ctgatgtata atgtaaaatc aaaccatcat ctgactttaa ccattcaatg
    31501 tcaaattcat cgctagcagt ttccatcaaa ctaatgaagt ctatgtaatt gtcttctaca
    31561 tcctcatcta ctacaacaat ttttttcaag tccggtaatt tatctacagg tactcgaggt
    31621 aacaatgcct tattagtaat taacacttta gcttcactgt tctctaatct atccgcaact
    31681 gccttttcca taaatgcttc aaataacggc ccaacaattg caccaatttt taaaatacct
    31741 aacaacgcaa aatatagttc aggtgtacgc gacataaata taaatactct gtcacctttg
    31801 tcaacttctg catgttcaga caaaacattc gctgctttat tagataaccg ttgcatatct
    31861 ttataagtat acgattcttt tctgtgctca tctttgtaat ttaacgctat tttatcccct
    31921 gatccttgat ctacatggcg atctatacat tcatatgcca tgttcatttt tccagtttca
    31981 ctccaagaaa atgcttgttc tacgtctttc caatcaaaag tattatatgt ttcttcataa
    32041 tctttaaggt tatgtttgcc ttgcactcct ttataaactt cgactttcat ttgaaactcc
    32101 ccctttgtta tgtgaaaacg ctttctttct caatattata cataacattt ctaattattc
    32161 aaaattttta cttttatcct ttttctagta ttaattattg taaattttct atataatgaa
    32221 tttaatacaa tcacgacgtc gattgaggag ttgaaaatat gaatcattta aagacgtatc
    32281 aatgcgaaga ttattacatt catggcaagc aatttgttat tgaaggtcct ttaacatacg
    32341 aagatttgaa agcgcttact ttcgatgcgc atttaaccgc atttagagat gctgaagatc
    32401 agtatgaagc tttgttagaa attacaacat taccagaagg tagaatttat gttgctcgcc
    32461 aagatcaact cattgtgggt tatgtcactt tccactatcc tgatgaaatt gagcgctggt
    32521 ctacaggtaa ccttccatat ttaatcgaat tgggagcaat tgaagtcagc atcaatttta
    32581 ggcaattaca acttgcagaa aagctgatac aacttagcct ttctacacca gaattcgagg
    32641 attatatcgt tataactaca gaatattact ggcattggga tttaaaaaat tcaaagttag
    32701 atgtatttga ctataaaaaa ttaatgcagc gattaatggc aactggtgga cttgaaatat
    32761 tcgctacaga tgatccagaa ataacaagtc atccagctaa ttgtttaatg gcaagaattg
    32821 gcaaaaatat tacattagaa cagcaacaag cgtttgatga tattcgatat atgaatcggt
    32881 ttttctttta atttttggaa tatcggaggg attatatgca acaacattca tcaaaaactg
    32941 catatgttta ttcagataag ttattacaat atcgatttca tgaccaacat cccttcaatc
    33001 aaatgcgttt aaaattaaca acagagctac ttttgaatgc aaatttattg tctccagaac
    33061 aaatagtaca acctagaatt gcaacagatg acgaattaat gttaattcat aaatatgatt
    33121 acgtcgaagc tattaagcat gcttcacatg gcattatcag tgaagatgag gctaagaaat
    33181 atggattaaa tgatgaagag aatggtcaat ttaagcatat gcatcgccat agtgccacaa
    33241 ttgttggagg cgctttaact ttagcagatc ttattatgtc aggcaaagta ttaaatggct
    33301 gtcacttagg tggtggtttg catcacgctc aacctggtcg agcaagtggt ttttgtatat
    33361 acaatgatat tgcaattacc gcacaatact tagctaaaga atacaatcaa cgcgttttaa
    33421 tcatagatac cgatgcacat catggagatg gtacacaatg gagtttctat gccgataacc
    33481 atgttactac ttattctatc catgaaaccg gaaaatttct tttcccaggc tctggtcact
    33541 atactgagcg cggtgaagat atcggctatg gacacactgt aaatgtccca cttgaaccgt
    33601 atacagaaga cgcatcattt ttggagtgtt ttaaattaac agtagagcct gtcgtaaaga
    33661 gttttaaacc tgatattatt ctaagcgtaa atggtgtcga tatacattat cgtgatccac
    33721 taactcatct aaattgtacg ttacattcat tatatgaaat tccatatttt gtaaaatatt
    33781 tagctgatac ttatacgaat ggaaaggtaa ttatgtttgg tggcggaggc tacaatattt
    33841 ggagagtcgt accacgtgca tggagtcatg tattcttaag tttaattgat caaccaattc
    33901 aaagtggtta tttaccgtta gaatggatta ataaatggaa acattattca tctgaattat
    33961 tacctaaaag atgggaagat cgtttaaatg attataccta tgtcccacgc acaaaagaaa
    34021 ttagtgaaaa aaataaaaaa ttagctttac atatagcgag ttggtacgaa tctactcgtc
    34081 aataagtcac ctaatctgca acgcttttaa aagataaata aaaggcattc ttccaacacc
    34141 cgtataaatc gggtcgttag atgaatgcct aattttgtga acttattttg tagttcctcg
    34201 gtattcaatt ctgtgtggta aaactacatt cggttcttct atcttttcat cgttcatata
    34261 ttttgttaat aagcgcatcc ctactgcacc gatatcatat aatggttgaa taacactaga
    34321 aagttgtggt ctaaccatct caactaagcg tgtattattg aaactaataa tttgtaattc
    34381 ctctggaact ttaataccag catccattgc actatgcatg ataccaatag cttcttcgtc
    34441 actgatacat aatatagcat ctggaagatt acctttcatt ttagcaaaag cttttacgcc
    34501 ttctttataa ctttcagcac cagaacaatt caatgtatca cctaattgaa gaccattttt
    34561 atttaacact tcagttaaac ctgctaaaac atcttcttga gcttttttag aatgttctcc
    34621 acctactaaa gcaaatgatt tagcgccttt ttcaattaat tctcccgtaa tttctttcgc
    34681 agcttcagta aaatcaatat taacagatgc tatatgtgca tccttaccat ttgttcctga
    34741 tactactaca ggtacagatg attgatttat caattctttc atttcttcag taattgtacc
    34801 accaaggaaa ataataccat caacttgttt acttaataag ttattaaaaa tttctttttc
    34861 cttttcagga tcgttatctg aatttgaaat aattgagtga tatttataca ttgttgcaat
    34921 atcttcaagt ccacgagcaa gttgcgaata atagatatta gatatatctg gaatgatcac
    34981 acctactgtt gttgtctttt tactagctaa acctctagca acagcatttg gacgataatt
    35041 caaacgctta atgacttcgt taactttatt tttagtttct gctttaacat tttggttccc
    35101 attaacaaca cgcgacactg tggccataga gacacgcgct tctcttgcta catcatatat
    35161 agtaactgtc ataatttcct ccttgtaaac gttttattca ttataataac atgttttcat
    35221 ttcattttca tagtttacca tattttaatt tacagtaaca tatttttgtt tggaaaaatt
    35281 tagcgaaaag cttgaaataa aaacaaacgc taaatgataa cgaacgatat attttaattt
    35341 tatataaact tttttcattt ttaattgtaa tattttgaga aagccttttc aaatatcaat
    35401 tacatttgtt gaaaaatgtt cacatttcag taacaaatgt gtgaatctat tatatttaca
    35461 gataattcat agtcataatt ttaattactg ctatcagtca tagtctttaa taattgcgaa
    35521 attggacaag tgtcgaagta ttataacaat cgttaagtgt agtatagcct ttatttctat
    35581 acaaaaaata atagccactc taaaagtaat atactcttag aatggctact agtttaaaat
    35641 tgtcgattca aagataatat tctacttcat catgatatat tagttaagta gtctatagtt
    35701 tccttggaat attattttaa ctttttagcg ttatataaat cagctaaagg ctttaattca
    35761 tcataaaatg cttggaattc atctaaatcc atttgttgac ccgcatcact aagtgcaaca
    35821 gatggatctg gatgcacctc agccataaca ccatcagcac ctactgctaa tgctgctttc
    35881 gcagttggta acatgatatc tttacgacct gtactatgcg taacatctac catgactggt
    35941 aagtgtgtac cttgttttaa aattggtact gctgaaatat ctaaagtgtt acgtgtcgcc
    36001 ttttcataag ttcggattcc gcgttcacat aaaataatgt tttgattacc ttgtgaagca
    36061 atgtattcag ctgcataaac aaactcttca attgtagcag ataaaccacg ttttaataga
    36121 ataggctttt tcgtacgccc agcttctttt aataactcaa agttttgcat attacgtgca
    36181 ccaatttgga atacatctaa atattcgtcg gctacttcaa aatcattagg atttacaatt
    36241 tcactaacta catttaaatc gtatttatct ttaatctgtt taagtatttt aagtccttca
    36301 acacctaggc cttggaagtc ataaggcgat gtacgtggtt taaaagcacc gccacgaata
    36361 aacttttcac ctttggcaag taaattttta gctaccgctt ctacttgttc aaatgattca
    36421 acagagcatg gtccaaatac aaatgatttg ttgccatctc caataatacc cccattatca
    36481 aatgttacaa tcgtatcctc aggtttcaac ttacgagata cgtataagtg tttttcattt
    36541 tcagattttt gtaaatctgt agaggcttta aaaatttctt taaataattg cttaattgta
    36601 ttatcgttaa atggtccttt gttgctatca attaagtcgt taagcatttc tttttcgcgt
    36661 tgtggatcat agatacgtgt accttgtttt aatttttctt caccaatttt ttgagctaac
    36721 tcgccacgtt tagacaataa atctaaaatt tgatgattca gtgatacaat ctcacttctg
    36781 tatgattcta atttattact catccttact cacctctact aattatttca agtatattcc
    36841 actttaaagc aataaaatat aatgttaaaa aatattctaa
//
LOCUS       DAHHRR010000012        36659 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.012, whole genome shotgun sequence.
ACCESSION   DAHHRR010000012 DAHHRR010000000
VERSION     DAHHRR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36659
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.012"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..117
                     /locus_tag="NBI42_000513"
     CDS             <1..117
                     /locus_tag="NBI42_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HCD1776960.1"
                     /translation="YHLYDDRGLWLAFNNNEDYARYSEKYNDLISEFDNEDI"
     gene            104..799
                     /locus_tag="NBI42_000514"
     CDS             104..799
                     /locus_tag="NBI42_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HCD1776961.1"
                     /translation="MKISNFLMFVAIIFLLILCLFFNLTNDKPLKVAILDTGVNKASV
                     DSKVISKEFVKSSKGINKHGDCIASIIEFQNNIVIYDAKVLNNDGLGTVEDTISGIDW
                     AIKNDVSVINMSYGFTHNYEKLHKKIKEANSKGIIILVANGNDIFGQKEYPASYKETI
                     SIGVMKNKNSKSIFNSNGNADLYISSRNEIRKNFNNTSEATAIATNRLIKNYKNNLRN
                     ADKNEVISIIKQE"
     gene            complement(910..1148)
                     /locus_tag="NBI42_000515"
                     /pseudo
     CDS             complement(910..1148)
                     /locus_tag="NBI42_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121211.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1271..2011)
                     /locus_tag="NBI42_000516"
     CDS             complement(1271..2011)
                     /locus_tag="NBI42_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCD1776962.1"
                     /translation="MFNLENDLVTLKHILDQSHRIAFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTKPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNECHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISNFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            2218..3009
                     /locus_tag="NBI42_000517"
     CDS             2218..3009
                     /locus_tag="NBI42_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1776963.1"
                     /translation="MHERLNKGGNFTVNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIARKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            3377..3805
                     /gene="lacA"
                     /locus_tag="NBI42_000518"
     CDS             3377..3805
                     /gene="lacA"
                     /locus_tag="NBI42_000518"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCD1776964.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TVGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            3821..4336
                     /gene="lacB"
                     /locus_tag="NBI42_000519"
     CDS             3821..4336
                     /gene="lacB"
                     /locus_tag="NBI42_000519"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCD1776965.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            4349..5281
                     /gene="lacC"
                     /locus_tag="NBI42_000520"
     CDS             4349..5281
                     /gene="lacC"
                     /locus_tag="NBI42_000520"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCD1776966.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKSVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDQDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            5285..6265
                     /gene="lacD"
                     /locus_tag="NBI42_000521"
     CDS             5285..6265
                     /gene="lacD"
                     /locus_tag="NBI42_000521"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCD1776967.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            6287..6598
                     /locus_tag="NBI42_000522"
     CDS             6287..6598
                     /locus_tag="NBI42_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCD1776968.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            6604..8316
                     /locus_tag="NBI42_000523"
     CDS             6604..8316
                     /locus_tag="NBI42_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1776969.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            8334..9746
                     /gene="lacG"
                     /locus_tag="NBI42_000524"
     CDS             8334..9746
                     /gene="lacG"
                     /locus_tag="NBI42_000524"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCD1776970.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKMIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            10008..10877
                     /locus_tag="NBI42_000525"
     CDS             10008..10877
                     /locus_tag="NBI42_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1776971.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIITVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            11156..12157
                     /locus_tag="NBI42_000526"
     CDS             11156..12157
                     /locus_tag="NBI42_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCD1776972.1"
                     /translation="MQNKQILFNKVPEGMPQEDTFKIEDIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKPGETVVISAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKADFAKELAQAVPDGVDVYF
                     ENVGGTVGDEVFKYLNRFARVPVCGAISSYNNPEEDIGPRIQGTLIKKQVMMRGFLVS
                     EFANDFKAASEQLATWVQEGKIQSQVTIEDGFENAPRAFKNLFTGDNFGKQVIKVTE"
     gene            12391..13398
                     /locus_tag="NBI42_000527"
     CDS             12391..13398
                     /locus_tag="NBI42_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD1776973.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISQNPSENIGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDY
                     IFCTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHR
                     TPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVIN
                     I"
     gene            13706..15268
                     /locus_tag="NBI42_000528"
     CDS             13706..15268
                     /locus_tag="NBI42_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCD1776974.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATFTPLLGKKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIAIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLLKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            15443..15979
                     /gene="amaP"
                     /locus_tag="NBI42_000529"
     CDS             15443..15979
                     /gene="amaP"
                     /locus_tag="NBI42_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCD1776975.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            15992..16231
                     /locus_tag="NBI42_000530"
     CDS             15992..16231
                     /locus_tag="NBI42_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCD1776976.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            16295..16804
                     /locus_tag="NBI42_000531"
     CDS             16295..16804
                     /locus_tag="NBI42_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD1776977.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
     gene            complement(16964..18940)
                     /gene="sfaD"
                     /locus_tag="NBI42_000532"
                     /gene_synonym="sfnaD"
     CDS             complement(16964..18940)
                     /gene="sfaD"
                     /locus_tag="NBI42_000532"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HCD1776978.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNNDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSHTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVFHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPMIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWIETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKVQLNELGYNTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            19042..20235
                     /gene="sfaA"
                     /locus_tag="NBI42_000533"
     CDS             19042..20235
                     /gene="sfaA"
                     /locus_tag="NBI42_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HCD1776979.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDATRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSLLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKLL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            20222..21979
                     /gene="sfaB"
                     /locus_tag="NBI42_000534"
     CDS             20222..21979
                     /gene="sfaB"
                     /locus_tag="NBI42_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HCD1776980.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYSQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            21986..23056
                     /gene="sfaC"
                     /locus_tag="NBI42_000535"
     CDS             21986..23056
                     /gene="sfaC"
                     /locus_tag="NBI42_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HCD1776981.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVQTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLYKVYID
                     DSKIDTLLQNFDVKSPAMVNQY"
     gene            complement(23157..23282)
                     /locus_tag="NBI42_000536"
     CDS             complement(23157..23282)
                     /locus_tag="NBI42_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776982.1"
                     /translation="MYHGYIIFELIQLTPFSMTVIPVIQLILSVTILCDELLHLI"
     gene            23449..24432
                     /locus_tag="NBI42_000537"
     CDS             23449..24432
                     /locus_tag="NBI42_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1776983.1"
                     /translation="MRGLKTFGILGLIVALFLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLIKKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADVNPERMIIMTDHAKKDSPEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            24444..25475
                     /locus_tag="NBI42_000538"
     CDS             24444..25475
                     /locus_tag="NBI42_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1776984.1"
                     /translation="MAIKQISSQSAIDYKRKRRTTLTYIVSVCFLFICVYLNMAIGSS
                     KINFSDIVHYLSGHTDTKASFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFVIVLVTIIIPSLEYYALYLGVIGAFVGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKLDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            25472..26440
                     /locus_tag="NBI42_000539"
     CDS             25472..26440
                     /locus_tag="NBI42_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCD1776985.1"
                     /translation="MMTAKMIRRYSLIVILLIISIFISLCVGSVMIHPIDAIKGIFTQ
                     DNFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MIFPTAPLFVLPIGSFVGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVVLAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            26675..28045
                     /locus_tag="NBI42_000540"
     CDS             26675..28045
                     /locus_tag="NBI42_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCD1776986.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLIPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGDVMPILIVTIITLSGILTLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVIFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPIMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(28177..28437)
                     /locus_tag="NBI42_000541"
     CDS             complement(28177..28437)
                     /locus_tag="NBI42_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776987.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            28614..29129
                     /locus_tag="NBI42_000542"
     CDS             28614..29129
                     /locus_tag="NBI42_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1776988.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            29173..30360
                     /locus_tag="NBI42_000543"
     CDS             29173..30360
                     /locus_tag="NBI42_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HCD1776989.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVKVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            30379..31062
                     /locus_tag="NBI42_000544"
     CDS             30379..31062
                     /locus_tag="NBI42_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCD1776990.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGLAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            31235..32578
                     /locus_tag="NBI42_000545"
     CDS             31235..32578
                     /locus_tag="NBI42_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1776991.1"
                     /translation="MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRV
                     IQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQL
                     LLLNFLSFILAIVVAIRLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVS
                     SIIISFGIKLPLLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
     gene            32677..33150
                     /gene="sepA"
                     /locus_tag="NBI42_000546"
     CDS             32677..33150
                     /gene="sepA"
                     /locus_tag="NBI42_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HCD1776992.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIHFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            33612..35054
                     /gene="lmrS"
                     /locus_tag="NBI42_000547"
     CDS             33612..35054
                     /gene="lmrS"
                     /locus_tag="NBI42_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HCD1776993.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLI
                     IDQASWRVPFIIIVGIALVAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPVVIGAMIISSIIIYLFIRRQFNISNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTSNEIKQQSIAIGVDISF
                     GFVAVLVMIAYVIAIFIREPEEIESNRRKF"
     gene            35202..36266
                     /locus_tag="NBI42_000548"
     CDS             35202..36266
                     /locus_tag="NBI42_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HCD1776994.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVV
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            36355..36541
                     /locus_tag="NBI42_000549"
                     /pseudo
     CDS             36355..36541
                     /locus_tag="NBI42_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12512 a         5115 c         6813 g        12219 t
ORIGIN      
        1 tatcatttat atgatgatag aggtctatgg ctagctttta ataataatga agattatgca
       61 aggtattctg aaaaatataa tgatttaata tcggaatttg ataatgaaga tatctaattt
      121 tttaatgttc gtagctatta tttttctact tattttatgt ttatttttta atctaacaaa
      181 tgacaaacca ttaaaagtag caatattaga tacaggcgtt aataaggctt ctgttgacag
      241 taaagtaatt tctaaagaat ttgttaaaag tagtaaagga atcaataaac atggtgattg
      301 tattgcaagc attatagaat ttcaaaataa tatagtgatt tacgatgcta aagtactgaa
      361 taatgatggt cttggcactg ttgaagatac cataagtggc atagattggg caattaagaa
      421 cgatgtaagc gtgataaaca tgagttatgg atttactcat aattatgaaa aacttcacaa
      481 aaaaataaaa gaggcaaaca gtaaaggaat tattatttta gtggcaaatg gcaatgatat
      541 atttgggcaa aaagaatatc ctgcgtctta taaagaaaca atctctatag gggtaatgaa
      601 aaataaaaat agcaaatcta tttttaattc aaacggaaac gcagatctat atatttcttc
      661 aagaaacgaa attagaaaaa actttaataa tacttctgaa gcaactgcaa tagctactaa
      721 tcgtttaatt aaaaattata aaaacaatct tagaaatgcg gacaaaaatg aagtgatttc
      781 tattataaag caagaataat tctcgctatc aaacttgtct agtgttggct aggtagaggt
      841 agtttttata ttgttgattt gtcaatttaa gtgcaacagt tcatcacata aaaattcatt
      901 aggaaatttc tattctaaac actttcttgg tcgataatta attgagcata atacatagtt
      961 aagttgctct tgattaactt tagctaagtc tgttttctta gggggaaaaa ttctcttagt
     1021 aaaccgttgg tattttaatt tgtatctttt tgccatgtgg aatatgaatc agagaagtag
     1081 acgttaatat taaattggtt ttcaatgttt tgataacaag agagctcttt ccctctaccg
     1141 acagttaagt tttaatgaat gaactttcat tgagtgagaa ggtctaaaag taacatccta
     1201 ttcaaataaa atgcaacttc aaaataaaaa gcatttccct accattcggg aaatgctttt
     1261 tacatactga ttactctgtc attaatgatt ttacaacgga aaccatgtcg tcatgtatga
     1321 ccaaagtagc gtcgctatca taaggtgtgc ggtctttatt gataataatt aaattgtcgc
     1381 ctttaaagtt tgatattaat cctgcggcag gttgcacaac gagtgacgaa cctaatacga
     1441 caagggtgtc agcatgttca attttattta atgcacgtat gatggtaggt tgatctaaca
     1501 tctcaccgta caatacgatg tctggtcgga tggcaccacc acaattatca caatgtttca
     1561 aagttctatc aatgacatca gacttcgtat aagatttatg acattcatta caataaaaac
     1621 gatttaacgt gccatgtaat tcatcaacat gttgacttcc agcgtctgag tgcaaaccat
     1681 cgatattttg cgtgatgaca cctaaagatt gttgattacg ttctaatttt gcaatccaat
     1741 catgtacgat attgggcttc gtatcgacaa acagtaagcg cttatgacag aaattgataa
     1801 aaccttcagg atcatcttct aaataatcgc gacttaataa gtattctgga gaaagcccat
     1861 cttttgaaat ttcatcaaat aagccaccca ttgaacgaaa gtcagggaca ccgcttgcaa
     1921 cagatacacc agcacctgtg aaaaatgcaa tacgatgcga ttggtctaaa atatgtttta
     1981 atgtcactaa atcattttcc aaattaaaca ccccatcaaa ataatgaatg atttataacg
     2041 tcttttgctt ttattatagt acatcaattt tccaattatc aaagtcgctg tttaaacagt
     2101 tctttttaaa aatgaattat acatataatc aaacagttaa aatatattag tttttacgca
     2161 atttttatta tagaataagc atgtatatta aaaaataatg tctaaacatt aaaggtaatg
     2221 cacgaacgcc taaataaagg aggcaacttt acagtgaata aacatgaacg tttggacgaa
     2281 attgctaaat tagtgaataa aaagggtacg ataagaacga atgaaatcgt tgaaggttta
     2341 aatgtgtctg acatgacagt tcgaagagat ttaattgaat tggaaaataa gggcatttta
     2401 acgaagattc atggtggtgc gcgcagtaat tcaacatttc agtataaaga aatatcacat
     2461 aaagaaaaac atacccgtca aattgcagaa aaacgattta tagctagaaa agctgcatca
     2521 ttaattgaag atggggatac tttgtttttc ggaccgggaa caacagtgga actattagca
     2581 gaagaagtca atcatcatac gctcacaatt attacgaatt gtttgccggt gtataaaatt
     2641 ttgttggaaa aacaaacagc acattttcgt gtctatttaa ttggtggtga aatgcgccat
     2701 attacagaag catttgtagg tgaaatggcg aatgccatgt tagaaaaatt aagatttagc
     2761 aagatgttct ttagtagtaa cgcagtaaat aaaggtgctg tgatgacatc tacattagat
     2821 gaagcctata cgcaacaact ggcactcagt aattcaattg aaaaatactt gctaattgat
     2881 catacgaaag ttggcaaaga agattttaca tcattttgtc agttaaacga attgactgca
     2941 gtggttatgg attatgaaga tgaagaaaag gtagaaacga ttaaaacata tattgaagta
     3001 gtcgactaaa ttaaaaatat aactgcattg ttaaaatatg tgtctatatg ctcataagtg
     3061 aatgtgaatt tgcattcatt tatgggctat ttttttgtgt gaatttacgt aaatgaacgc
     3121 cccttattac tcatacttca attacagacg aacacctaat taaaataacg ccacaacatt
     3181 aatgttcact tattgtttga aaatatgcca tattcaaaca aaatacaaac atattatgtt
     3241 gatttattgt ttgtttatgt tttaatttaa acgtaatcaa attgaaagcc ttttctcaag
     3301 attttcataa cctacaaata aacatgtcat attttaagaa aagcatttca aaattaaagt
     3361 aaaaaggagt cttattatgg cgattattat tggttcagat gaagctggca aacgtttaaa
     3421 agaagtcatc aaatcatact tattagacaa taaatatgat gtcgttgacg taacagaagg
     3481 acaggaagtt gactttgttg atgcaacttt ggctgtagca aaagatgttc aaagtcaaga
     3541 aggtaactta ggtattgtta tcgatgcatt tggtgcaggt agctttatgg ttgctacaaa
     3601 aatcaaaggc atgattgctg ctgaagtatc agatgaacgc tctggatata tgactagagg
     3661 ccataacaat tcaagaatga tcactgtggg atctgaaatt gttggagaca cattagcgaa
     3721 aaatgtcgtt aaaggttttg ttgaaggtaa atacgatggt ggtagacacc aaatccgtgt
     3781 agatatgctt aataaaatgt gctagggagg aaaaattact atgaagattg cattaggatg
     3841 cgaccatatt gttacagata caaaaatgcg tgtatctgaa tttttaaaat caaaaggaca
     3901 tgaagtcatt gacgtaggaa catacgattt cacaagaaca cattatccaa tttttggtaa
     3961 aaaagttggc gaacaagttg ttagcggtaa tgcagattta ggtgtttgta tttgtggaac
     4021 aggtgttggt attaacaatg ctgtaaataa agtacctggc gttcgttcag cactagtacg
     4081 tgatatgaca tcagcgttat atgctaaaga agaattaaat gcgaacgtca ttggctttgg
     4141 tggacgtatt ataggtgagt tattaatgtg cgatattatc gatgcattta ttaacgctga
     4201 atataaacca actgaagaga acaaaaaatt aatcgctaaa attaaacatt tagaaacaag
     4261 caatgcagat caagctgatc cacatttttt tgatgaattc ttagaaaaat gggacagagg
     4321 cgaataccac gattaaggga cgacacatat gattttaact ttaacattaa atccatccgt
     4381 tgatatttca tatccattaa cagcattgaa attagatgat gttaatcgtg ttcaagaagt
     4441 aagtaaaaca gctggtggta aaggtttaaa tgtaacaaga gtacttgcgc aagtaggtga
     4501 gccagtcctt gcgagtggtt ttattggcgg tgaattaggt caatttattg ctaaaaaatt
     4561 agatcatgcc gacatcaagc atgcatttta taacattaag ggtgaaacac gtaactgcat
     4621 tgcaatttta catgaagggc aacaaacaga aattttagag cagggaccgg agattgataa
     4681 tcaagaggct gcaggattta ttaaacattt tgaacaatta ttagaaaaag ttgaagcagt
     4741 tgctatttca ggtagcttac ctaaaggatt aaatcaagat tattatgcac aaatcattga
     4801 acgatgccaa aacaaaagtg tgcctgtgat attagactgc tcgggtgcaa ctttgcaaac
     4861 agttttggaa aatccatata aaccaacagt cattaaacca aacatttctg aactatatca
     4921 attgcttaac cagccacttg atgaaagttt agaaagtttg aaacaggcag tgtcacaacc
     4981 cttattcgaa gggattgagt ggattattgt gtctttaggt gcgcaaggtg cattcgctaa
     5041 gcacaatcat acattctata gagtgaatat tccgacaata agtgtgttaa atccggttgg
     5101 ttcaggtgat tctacggttg ctggaattac ttcagcaatt cttaatcatg aaaacgacca
     5161 agatttacta aaaaaagcca atactttagg catgctgaat gcgcaagaag cacaaacagg
     5221 ttatgtcaat ttaaataatt atgatgattt attcaatcaa attgaagttt tagaggtgta
     5281 aaaaatgtca aaatcgaatc aaaaaatcgc atcaattgaa caattaagta ataacgaagg
     5341 tatcatttct gcattagcat ttgatcaacg tggtgcttta aagcgcatga tggctaaaca
     5401 tcaaacggaa gagccaactg tagctcaaat tgaacaatta aaagtattag ttgcggaaga
     5461 attaacacaa tatgcatctt caattttatt agatcctgaa tatggtttac cagcttcaga
     5521 tgcacgtaac aaagattgtg gattattact tgcttatgaa aaaactggat atgacgtaaa
     5581 cgctaaaggt cgtttacctg actgtttagt agagtggtct gcaaaacgtt taaaagaaca
     5641 aggtgcaaat gctgttaaat tcttacttta ctatgatgtt gatgatgctg aagaaattaa
     5701 cattcaaaag aaagcatata ttgaaagaat cggttcagag tgtgtagcag aagatattcc
     5761 tttcttctta gaagtattaa catacgatga caacattcct gataacggta gtgttgaatt
     5821 cgcaaaagtt aagcctagaa aggttaacga agcaatgaaa ttattctcag aaccacgctt
     5881 taatgttgat gtattgaaag ttgaagtacc agtaaacatg aagtatgtag aaggctttgc
     5941 tgaaggtgaa gtggtttata caaaagagga agcggcacaa cactttaaag atcaagatgc
     6001 agcaactcat ttaccataca tttacttaag tgctggtgtt tctgcggaat tattccaaga
     6061 aactttaaaa tttgctcacg aagctggtgc taaattcaac ggtgtacttt gtggccgtgc
     6121 tacatggtct ggtgctgttc aagtatacat tgaacaaggt gaagatgctg ctcgtgaatg
     6181 gttacgtaca actggtttca aaaatattga tgacttaaat aaagtattaa aagatactgc
     6241 gacatcttgg aaacaaagaa aataagttta aaaggggatt aatgctatga atagagaaga
     6301 agtccaatta ttaggttttg aaattgttgc atttgcaggg gatgcacgtt ctaagttttt
     6361 agaagcatta acagcagctc aagctggaga ttttgcaaaa gcagatgcac tgattgaaga
     6421 aggaaacaat tgcattgcgg aagcgcatag agcacaaaca agcctgttgg ctaaagaagc
     6481 gcaaggtgat gatattgcat acagtgtgac gatgatgcat ggtcaagacc atttaatgac
     6541 aacaatttta ctgaaagatt taatgaagca tttattagaa ttttataaaa gagggtgatg
     6601 acaatgatgc aaaaattgat cgcacaaatt gaaaaaggga agccattttt cgagaaatta
     6661 tcacgtaaca tatatttaag agccatccgt gatggattta tttctgcaat gcctgtcatc
     6721 ttattctcaa gtatctttct attaattgca tacgtgccaa atatatttgg ttttaaatgg
     6781 gataaaggca tggaagccat tttaatgaaa ccatataact atacgatggg gcttgtagca
     6841 tttttagtag caggaacgac agcgaaatct ttaacagatt catttaaccg taagctagaa
     6901 agtacaaatc aaattaactt tatttctaca atgctagcag cgatgtgtgg attcttgttc
     6961 ttagcttctg atccagccaa agatggtgga ttcttaagtg catttatggg aactaaaggt
     7021 ttattaacag cattcttatc agcatttgta actgttattg tttataactt ctgtgtgaag
     7081 cgcaatatta caattaaaat gccgaaagaa gtaccgccga atatttcaca agtatttaag
     7141 gacttaattc cattttcagc ggtaatcatc attctttatg cattagattt agtcattcgc
     7201 aacagcttta aatcaaatgt agcggaaggt attttaaaat tattcgaacc attatttaca
     7261 gcagcagatg gatggattgg tgtcacaatt atctttggtg cctttgcatt attctggttt
     7321 gtaggaattc atggtccatc aatagtagaa ccagcaattg cagccattac atatgcgaat
     7381 atcgaagcga acttcaagtt gcttcaagct ggagaacatg cagataaaat tattacatca
     7441 ggtacacaaa tgtttatcgt tacatttgga ggtacgggtg caacattggt cgtcccgttc
     7501 atgtttatgt ggatgacgaa atctaaacga aacaaagcga ttggacgtgc atcagttgta
     7561 ccaacattct ttggtgtaaa cgagccgatt ttatttggtg caccactagt attgaatcct
     7621 gtgttcttta ttccatttgt attagcgcca attgtgaacg tatggatttt caaacttttc
     7681 gttgaagtgt taggaatgaa cagctttagt gtgaacttac cttggacaac accaggtccg
     7741 ctcggcatta tcatgggtac aggttttggt ttatggtcat tcgtactagc tattactttg
     7801 attgttgtag atattattat ttactaccca ttcctaaaag tttatgatag tgaaattctt
     7861 gatgaagaag aaggacgtaa agaaagtaat tcagatttaa aagaaaaagt tgcagcaaac
     7921 tttgatacga aaaaagctga ttcaatttta gcggcaagtg gtgtatcaga cgatgcagct
     7981 aaagcttcaa atatcacaga acaaacaaat gtcttagtac tttgtgccgg tggtggtaca
     8041 agtggattat tagccaatgc acttaataaa gcagctgaag aatatcatgt gccagtgaaa
     8101 gcggcagctg gcggatatgg cgcacatatg gacattatga aagagtacca attaatcatt
     8161 ttagcgccac aagttgcgtc taactatgaa gatattaagc aagatacaga tagattaggt
     8221 attaaattag cgaagactca aggtgccgaa tatatcaaat tgacacgaga tggccaagca
     8281 gcccttgatt tcgttcaaca acaatttgaa aattaagaat aggagttttt catatgacta
     8341 aaacattacc tgaagatttt atttttggtg gagcaacagc agcatatcaa gcagaaggtg
     8401 cgacaaatac agatggtaaa ggtcgtgtag catgggatac gtatttggaa gaaaactatt
     8461 ggtacactgc agaaccagcg agtgattttt ataacagata tccagtggat ttagaattaa
     8521 gtgaaaaatt cggtgttaat ggtatccgta tttcaattgc atggtctcgt atcttcccaa
     8581 atggatatgg cgaagttaac cctaaaggtg ttgagtatta ccataagtta tttgcggagt
     8641 gtcataaacg tcatgttgaa ccatttgtaa cattgcatca ttttgataca ccagaagtgt
     8701 tacacaaaga tggcgatttt ctaaatcgta aaatgattga ctactttgta gattatgctg
     8761 aatattgttt taaagagttc ccagaagtaa aatattggac tacattcaat gaaattggac
     8821 caattggtga tggtcaatat ttagtaggta aattccctcc aggtattaaa tatgattttg
     8881 aaaaagtttt ccaatctcat cataatatga tggttgctca tgcaagagca gtaaaattat
     8941 ttaaagatgg cggctatcaa ggagaaattg gtgttgtaca cgcattgcca acaaaatatc
     9001 cgttcgatcc atcaaatcct gaagatgttc gtgcagcaga attagaagat atcatccata
     9061 ataaattcat cttagatgca acatatttag gtaagtattc tcgtgaaacg atggaaggtg
     9121 tacaacatat cttatctgtt aatggcggta aattaaacat tacagatgaa gattatgcca
     9181 ttttagatgc agccaaagat ttaaacgact tcttaggtat caattactac atgagtgact
     9241 ggatgagagg ttacgatgga gaatctgaaa tcacgcataa tgcgacaggt gataaaggcg
     9301 gttctaaata ccaacttaaa ggtgtcggac aacgtgaatt tgatgtcgac gtacctcgca
     9361 cagactggga ctggatgatc tatcctcaag gtttatatga tcaaatcatg cgtgttgtta
     9421 aagattatcc taactatcat aagatttata tcactgaaaa tggtttagga tataaagatg
     9481 aatttattga atctgaaaaa acagttcatg atgatgcacg tattgattat gtaagacaac
     9541 atttgaacgt tattgcagat gctattaaag atggtgcaaa tgttaaaggt tactttattt
     9601 ggtcattaat ggatgtgttc tcttggtcaa atggttatga aaaacgatat ggtttattct
     9661 atgtcgattt tgagactcaa gaacgttatc ctaagaaaag tgcttattgg tacaaagagt
     9721 tagccgaaac aaaagaaatt aaataattag agatattaaa aggcctatca gtcgagtgct
     9781 ggtaggtttt tatttataga aagagataaa ttaaacatgc atatacatat atattaatca
     9841 atgggtgaca caccattgaa agctaaagaa gcaatcaatt aacaaacgtt aacagacaac
     9901 tttccaaagt catcttaaaa gttaatgatg tattcaaaat caactaaagg acatttttaa
     9961 tcacatcctt tatacttata taaaagacat gaatgggagt taaaagtgtg aagaaaattt
    10021 ttgtaattat tacgacattg ttagcggtcg ctattattat aggctctatt attacggtgg
    10081 tgtttagtca acgacaggca caaacattta aaatacaaca acagcaattt gttaaaaagc
    10141 caattccaac attgttctta catggatttg gtggtagtgc caattctgaa aagtttatgg
    10201 tgaagcaagc agaaaaaaga ggggtaacta aagatatcat cactgcttac gtttcaaagg
    10261 atggtgcagt gacatttaaa gggaaactaa gaaaagatgc agtgaatcct attgtgaaaa
    10321 ttgaacttga aaataacaga caaggctatt tggataagaa tgctgcgtgg tttaaaaatg
    10381 tactgactaa gttacaatct gaatacaatt tcgataaatt caactttgtc ggtcactcaa
    10441 tggggaactt aacatttgca caatatatga tgacatatgg taacgacaag tcactgccac
    10501 agttaaacaa acaagtgaat attgcaggga catttaatgg tgttttaaat atgaatgaag
    10561 atgtcaatga aattacagtt gataaagatg gtaaaccaag tcgaatgaat caaccttatc
    10621 agcaattacg cgtgctaaaa gatatttata aaggtaaagg tatcgaagtg ctaaatattt
    10681 atggagatct gaaagatggt acacattcag acggcagagt gtctaatagt tcttcgaaat
    10741 cattaaaata tttactggga aatagtccga aaagttatcg agaatctaaa tatgaaggtg
    10801 agcctgcaca gcatagtcag cttcatgaga atgaaaatgt tgctaatgag ttgatcgact
    10861 tcttatggaa aaaataaagc aactactagt ttttctgggg taaaaaagct tgtaatatta
    10921 gagcttttca aaaattattt attgtttgag agtttccata aaatgtctaa gtcgtaacat
    10981 ttttaattta tgtttatagc aaatataatg tacttgaagt taaatgaatc agtgcttaag
    11041 tcaaaatata acaactaaga atgtatttgc atacagacat cacgaagaca caattaaaac
    11101 ttaacgctta agtaacaaat aagtaaaagt ttcaaataaa gaaaggtgat taatgatgca
    11161 aaataaacaa attcttttta ataaagtacc agaaggcatg cctcaagagg atacattcaa
    11221 aattgaagat attgacacgc ctaaacttga gagcgatggt gtacttgtac aaacgctgta
    11281 tatttcagtg gatccatata tgagaggacg tatgactaag gcagattcgt atgtacagcc
    11341 ttttgaaatt ggtaagccga ttgttagtca tgtcgttgca aaagtaattg attcgacact
    11401 cgcagattat aaaaaaggag atgtcgttgt aggtatgttg ccttggcgaa tcattaatca
    11461 tgtccaagcg gatcaaatta ctaaagtacc aacaacagat gtaccacttg acttatattt
    11521 aagtgtctta ggtatgcctg ggcaaacagc atatcatggg ttgctcgata ttggtcaacc
    11581 taaaccaggt gaaactgtag ttatttcagc tgcgtctggt gctgttggtt cagttgttgg
    11641 tcaaattgcc aaaattaaag gttgccgtgt tgtaggtata gctggtggcg ataaaaaagt
    11701 aaactattta acggagacat taggatttga tgctggtatc gattataaga aagcagattt
    11761 tgcaaaagaa ttagcgcaag cagtaccaga tggtgttgat gtttattttg aaaatgttgg
    11821 cggtacagtc ggtgatgaag tatttaaata tttgaatcgt tttgcaagag taccagtttg
    11881 tggcgcaatt tcttcatata ataatcctga agaagatatt ggtccacgaa ttcaaggcac
    11941 actgattaaa aagcaagtta tgatgagagg tttcttagta tcagaatttg ctaatgattt
    12001 taaagctgca agtgaacaac ttgcaacatg ggttcaagaa ggtaaaattc agtcacaagt
    12061 aaccattgaa gatggttttg aaaatgcgcc acgtgcattt aaaaatttat ttactggcga
    12121 taactttggt aaacaagtta ttaaagtaac ggaataataa agtataagtt ttaacaaacc
    12181 aactttgtta attgaccaaa atttaataat tttttatcta aagcaaattt gttcagtaac
    12241 atggtagaaa taagtctaaa atttaatacg aaagtcgtct gtaattattt gtatattcaa
    12301 atgaaaaagg agtcatgatg ctgtgatata agcacacatg gcttctattt tgtataataa
    12361 gaaataagtt cataaatgag gtgaaacgat atgaaaatga taggatttga aaagcccttt
    12421 aaattggaag aaggaaattt atttaaagta tacgaacaaa gaaagccaac gccagaaaac
    12481 gatgatatct tagtcaaagt aaatagcatt agtgtaaatc ctgtagatac aaaacaacgt
    12541 caaatggaag tcactcaagc acctcgtgta cttgggtttg atgctattgg tactgttgaa
    12601 gctatcggac cagacgttac attatttagt ccgggtgacg ttgtatttta tgctggatcg
    12661 ccaaatagac aaggatcaaa tgcgacatat caattggtgt cagaagctat agttgcaaaa
    12721 gcaccacaca acataagtgc taatgaagcg gttagtctac cgctaactgg tattacggca
    12781 tatgaaacgt tttttgatac atttaaaatt tcacaaaatc cttctgaaaa tataggtaaa
    12841 tccgtgttaa tcattaatgg cgctgggggt gtaggaagta ttgccactca aattgctaaa
    12901 cggtatggtt taactgtcat tacaacagca tcacgacaag aaacaacaga gtggtgtgaa
    12961 aagatgggcg cagatatcgt attgaatcat aaagaggatt tagtgcgtca atttaaagaa
    13021 aaagaaattc cattagtaga ttatatattt tgcacctata atacagactt gtattacaat
    13081 actatgattg aactgataaa accattaggg catattacaa ctatcgtagc ttttaatgaa
    13141 gatcaagatt taaatgcact aaaattaaaa agtataacat ttacacatga atttatgttt
    13201 gcgcgtccaa ttcatcgaac gcctgatatg attaaacaac atgagtattt agaagatatt
    13261 acgaaaaata tagaatcagg gcattaccaa ccaacaacga cacaagtttt tgaaggttta
    13321 tcgcctgaaa acttatatca agcacatcaa ttattggaaa aacagtcaat gattgggaaa
    13381 ttagtaatta atatatagtt tagtaagtaa agtcgcctta tgcagaaaat actgtatagg
    13441 gcgacttatt ttttgtgtct agcgactatt gattctaaac ggaaatatgg caactttgca
    13501 ggtatcaaat caataatgca ctagaaaggc attacttaaa tacaataaaa tactttgttg
    13561 ctcaatgata aataatttga gtgtgtttta tatatcattt ttaaaagatg aagcattatt
    13621 aattgtgtct atataaaatt acaaatgatt aaaatgttgc agttatgggt atgaacttac
    13681 cattacacag aaagggtttg tatttatgaa aaataaatca gtaagtctag tgttttgggt
    13741 atctttagtg atatgtacga tatttgttgc ttttggagca atatttccaa aacagttaga
    13801 aaaactcacg caaaacatta ctacatttat tgcgctacat ttttcttggt attatttatt
    13861 gctcgtttta gttattttat ttgtctgtgt gtatatttta ttttcaagat acgcaaacat
    13921 tacattaggt gaagaaggcg aagatcctga attctcattg ccgtcttggt ttgctatgtt
    13981 atttagtgct ggtatgggaa taggacttgt gttctggaca acagcagaac caatcagtca
    14041 tgccttcaaa ttaacgccta ttcataaagc tggcacgcaa tctgcaatta atgatgcaat
    14101 gcagttttca tttttccatt gggggattca tgcatgggca gtctatggta ttgtcgcgct
    14161 tgtgtttgca tattttagct ttcataaagg atatcctggc ttagtcagcg ctacatttac
    14221 gcctttatta ggtaaaaagg caatgcgtgg ccctttaggt ggtgccattg atgtattggc
    14281 agtcatagct acagtaacag gcgttgctgc aacattaggt ttcggtgcat tgcaaataaa
    14341 cgaaggttta cattttttat ttaatgtacc gagtaacttc acgatgcaag taatcttgat
    14401 tgccattgca accatattat ttacttggtc agcatggtca ggtatcgata aaggtatcaa
    14461 aacgttgagt aatatcaaca tgttacttgc ttttgtagta cttattggtc tttttattgt
    14521 tgggccaacg ctttacattt taaatacatt tacgaatggt ctaggtaatt atattgcaaa
    14581 tttctttagt atgagtttac gcattccgtc tggcggtcaa aaattccaat ggttacaaaa
    14641 ttggacaatc ttttactggg catggtggat atcttgggca ccatttgtag gtatatttat
    14701 tgcacgtgtg tcaaaaggac gtacgattaa agaatttatt ctaggtgtac tttttgtccc
    14761 ggcgttagtt tgctttatat tctttgcggt attcggcgcg tcagcaattt acttacaaga
    14821 taatcatatt gccgatattg ctaaagctgc gacagaaaca gcaacttttg caacgctaca
    14881 acattatccg ctaggttttg tattaagctt aattacttta cttgttatta tgattttctt
    14941 tgttacatca gcggattcgg caacttatgt tttaggtatg ttgagttcaa gtggtgatat
    15001 taatccgaag tcatttgtca aagtgagttg gggtatcatt atggcattat ttgcaattat
    15061 catgatctac acaggtggta cgcaagcgat tcaaaattta ttaatcattg ctgcgttacc
    15121 attttccgtg gtcattatag cgatgatatg gtcgctttta aaatctttga gtgaggagaa
    15181 accgagaaat agtaataagg ttttaattaa acatcgtgac ccagatattt tagaatatcg
    15241 ttcttcaaaa catattgatg aaaattaatt aataatgagg ttttatttac aaaaactagg
    15301 gaatatatta ctaaatggta cttcatctta tatattaaaa ataaatttaa agttattaat
    15361 ttattatgtt taagaaaaaa actgatgggt acatatttaa tatgttttta aattaacttt
    15421 aactaaggag gtgtcgttag gggtgaaacg acttaaaaac tttatccttg gcttattaat
    15481 agtagtaatc gtggggttcc tgttattcat gtatatccaa gatggacgta taactgaata
    15541 tcaagactac ttccttcaat ttgaatggtt ccaaccatta cttatttcac ttgctgcact
    15601 attgatatta ataggtctta tattagtatt tagtatcttc aaacctacgc atcgaaaacc
    15661 tggattatac aaagacttcg atgatggtca tgtttacgta tctcgtaaag ctgttgaaaa
    15721 gacggcattc gatacggttg caaaatatga tcaagtaaga caaccaaatg tagttgcgaa
    15781 actatataac aaaaaaaata aatcttatat cgacattaaa actgactttt tcgtaccaaa
    15841 taatgttcaa gttcaatctt taactgaagc aattcgttcg gatattaaaa agaatgtaga
    15901 atatttcaca gaaatgccag tacgtaagtt agaagttaat gtgagagatc agaaaacttc
    15961 tggtccacga gtgttgtaag ggaggtatat catggctaac aatcataacc aaaacggaca
    16021 agactctaca caacaagtga ttaatttttt aaaagtattt aaatggagaa tcgttggttt
    16081 cctagcgttt ctattaatcg cgatattatt cttaacgtta ggtttttgga aaacagtact
    16141 tatcatcgtt ttatgtttaa ttggtgtagg tattgggtat atgaaagacc gtaagcaaga
    16201 ttttatgaat tttttaaatc gatggagtta attcatcaat ttagaattta attaaaaaaa
    16261 tataaaaaaa caataagtta aaggagaatc tattatgact gtagataaca ataaagcaaa
    16321 acaagcatac gacaatcaaa ctggtgttaa cgaaaaagaa agagaagagc gtcaaaaaca
    16381 acaagaacaa aatcaagagc ctcaattcaa aaataaatta acattctctg atgaagttgt
    16441 tgaaaaaatt gctggtatcg ctgcacgtga agttaaaggt atcttagaca tgaaaggtgg
    16501 cttaactgat acattcacta atgcattctc aagtggcaac aacgttacac aaggtgtatc
    16561 tgttgaagtt ggtgaaaaac aagctgctgt agacttaaaa gtaattttag aatatggtga
    16621 atcagcacct aaaatcttcc gtaaagtaac tgaattagta aaagaacaag ttaaatatat
    16681 tactggttta gatgttgttg aagttaacat gcaagttgac gatgtaatga ctcaaaaaga
    16741 atggaaacaa aaacacgaaa aaaataacga aaacaataac caagaaagac aaggtttaca
    16801 ataagttatt cgataaaaag ctcggcactc aatttaagtg ccgagctttt ttgtgatttt
    16861 taataatttg aatatggaaa atgtattatt ctctcatttg tatagattgt atttaataag
    16921 ttaatgtaat ccttgagctc acgattaata aatttctata accttaatta ttttctcgat
    16981 acaaaggatt attaacttta atataagtat aatgatgtgc ttcatcttca agacgcattg
    17041 ttgtaacaca cttataatct ataaatggcg cgaacatggt atctttaatt tcatttatgc
    17101 gattctcatt tacttgatta gatttgtgtg tcgaaagtac aagttgatca aaaatgttat
    17161 ctagtacatc acgaacaata taccacatat gtctttctaa gtttgaatca tttgatgctt
    17221 tagagattgt aagaattaat tcgcctaaat ggttttgaac ggtagaataa aaggctttgt
    17281 taaacacaga cgtttttgaa tcagtaagaa ttcttgattt ttcatggaaa tgagatgtat
    17341 tgtatcccag ttcatttagt tgtactttat caattcttaa accttcaaag tctctaatat
    17401 acatcgtgtc caatagacca tctttccgaa atgttgcaat agcattttgc aagtgagcct
    17461 ctaatgcaat tccatattta gtaactagtg ggattacgag accaagcaat gctttactat
    17521 aagtttcaat ccacgatttc gccgatgatt caaaatcaga caatgatgca gctgattgat
    17581 aacgtttaat caatgtcacg ataggtgatt cattattaaa tgggtaggtt gcaactaagc
    17641 ttgaaggaat cattggtgtg acttcttgtg gaatcatttg gtatatgttt tttctaaata
    17701 atgtacctaa ttgttcactt cgttcagttt gatagtctgc ttcgtcttgc tcattataaa
    17761 aatgaatacc agcaacttca tcaataattg ttgatgcata ggacttaaat atgacatctt
    17821 tctccaaaat atcatttaaa atacgtgtca ttagtggacc attgtgcgtc gtttgttctg
    17881 ataatgtacg aatttcacct gtaatatgaa cgtttgtcga caatttgatg tgtggcgaca
    17941 tagctgggta tttaggaact aatgttctga aagataaacc ggcataataa tccaacgtat
    18001 gttttgcttc aatgattaat tctttatcta cttctgcttg ataatcagaa tgaaatacgt
    18061 catctaattg ccatggatgt acaatcataa tgtgataatc attaaagtta aactttggcg
    18121 taaattcatt ttctaattgt ttaattaagt ccggaaatag ttgatgaaca gttgtatcat
    18181 aatctttaga cagtgacata gtatgactta atttactgtg aatcagtact attttcaact
    18241 taataggttg attgaattct gaagaatata agaatgtttg taatgcattt aaacctttac
    18301 gtaatttagc cccgggatgt agcggatgac cttcaataac ggcttgctct gaacgcaagt
    18361 aactatcttc gctattttcg ataatattaa ataaaggtgc agaatcatgt tgcattgaca
    18421 gtgcttgata gctaattgca aatgtcatat tagttgcact gtttattaaa tcttgctgaa
    18481 attgatcact agcagcattt tttaaatctg gtgcttcaat taaaatacac tcaagaattt
    18541 catttggatg gtgtactcgt gtaatcgtat ttgtaatgtc atctttaatg tagaaagggc
    18601 cttcaacatc aattcgatca aaggcgtgtt ctccagtgat aggagcatat aatgtttgct
    18661 tagcttgtgg gaactggatt tctagtatat gagtcgttga gatatctaac ataatcaaat
    18721 cacgactcaa tattttctta ctttgagtgc gcgctttaac taagttttcg cgatgcattg
    18781 atgtgactaa tctctgagtg actttatctc ttccttgtaa aatcatctct ttaaaaatat
    18841 tagcccaatc gttattatgt tgttgtaaaa ataaatatgt ttcttgttct tctttgttaa
    18901 attttagtgt ctgttcttta aaaattaagt tcaagttcat aattcacctc tatgaaatat
    18961 tttacaaaag caagatagat ttgtataatc catattaatg ataatgattc ttattatcaa
    19021 cagaatgcgg gtgtaagttt tatgacaaaa tattttttta gcagttcttt tctactattt
    19081 ctaggtaatt ggattggaca aatagggcta aattggtttg tgcttaccac ttatcataac
    19141 gcagtttatc tggggattgt caatttttgc agacttgtac caatattatt actaagtgtg
    19201 tgggcagggg caattgccga taaatatgat aaagggcgat tgctgagaat tactatttca
    19261 tcatcatttt tagtaactgc aattttatgt gtgcttacgt atagtttcac tgcaattcca
    19321 attagcgtca ttattatata tgcgacatta agagggattt taagtgcggt tgaaacacct
    19381 ttaagacaag caatcttacc agatttatca gataaaatat ctacgacaca agctgtatca
    19441 tttcattcat tcatcattaa tatttgtcgt tcaatagggc ctgccattgc tggtgtcata
    19501 ttagcagtct atcatgcacc aacaacattt cttgcacagg caatttgtta ttttatcgca
    19561 gttttattat gcttaccatt acattttaaa gtaactaaaa tacctgaaga tgcaacaaga
    19621 tacatgccgt taaaagtcat tatagattac ttcaaattac atatggaagg tcgacaaata
    19681 tttataacat cattattgat tatggccaca ggtttttcat atacgacact tttaccagtt
    19741 ttgacaaaca aagtatttcc ggggaaatct gaaatatttg gtatcgctat gacgatgtgt
    19801 gccattggtg gtattattgc aacgctagtt ttacctaaag tacttaaata tattggtatg
    19861 gtaaatatgt attatttaag ttcactttta tttggtattg ctttgttagg tgtggtattt
    19921 cacaatattg tcatcatgtt catttgtatt acattgattg ggttatttag tcaatgggca
    19981 cgtacgacaa atcgcgttta ttttcaaaat aatgttaaag attatgaacg tggtaaacta
    20041 ctgagtatta ttatgatgga tagaggtatg attccattgg gaagtctgtt aatgagtata
    20101 tgtgcagatg tgtttggcat tgttagaact ttttcaataa tgggaataag tactatatgc
    20161 attacaatgg tattctattt tataaataga aagttgaagt taaagttgga ggaaagtaat
    20221 catggtatat cttgaatggg caaaggcaga tagaaatatt caatatcgtg taattaacgc
    20281 cattattaaa gaacgtattt accccgagca aacatttatt tcgcaaaaag gatctttaat
    20341 tgaaattcag tatcatatgc atgtgttgac tattgaagtt gttagaaaaa gtgcattaga
    20401 acgctatgag tttacaggtg atattactta tttaaataaa ggtgaaacgt cattaattat
    20461 aactttagaa ggtttgttag atgtgttgaa tcatgacttt gatatcccta tttcagagcg
    20521 actacgcgaa gagttaatac acagtcgaga tagtttagtt gaaacatata agcaaatgtc
    20581 tcacagacaa acattaataa gtcaaagttt taaattttca agattaccac aagatattaa
    20641 ctttttttca tggttacaac atgtaaaaga tagtgataag acagatgatt taacttattc
    20701 tgagagtttg gtaccagagg ggcatccaac acacccttta accaaaacga aattgccctt
    20761 aactatggaa gaagtacgag catatgcacc tgagtttgaa aaagaaatcc ctttgcaaat
    20821 tatgatgatt gaaaaagacc atgttgtgtg cacagctatg gatggtaatg atcaatttat
    20881 tattgatgaa ataattcccg aatactataa tcagattcgt gtgtttttga agagtttagg
    20941 tttgaaaagt gaagactata gagcgatttt agtacatcct tggcaatatg atcatacgat
    21001 agggaaatat tttgaagcat ggattgctaa aaaaatatta attccaacgc cgtttacaat
    21061 actttcaaaa gcaactttat catttaggac gatgtcttta attgataaac catatcatgt
    21121 taagttgccc gtcgatgcac aagcaacaag tgccgttaga acagtctcaa ctgtgactac
    21181 tgtagatgga ccaaagttaa gttatgcttt acaaaacatg ttgaatcaat atccaggatt
    21241 taaagttgct atggaaccgt tcggtgaata tgcaaatgtt gataaagata gggcacgtca
    21301 gcttgcttgt attataagac aaaagcctga aattgatgga aaaggtgcaa cagtagttag
    21361 tgcaagtcta gttaataaaa atccaataga tcaaaaagtt atcgtggata gttacttaga
    21421 gtggttaaat caaggaatta ctaaagaaag tattacgaca tttattgaac gatactctca
    21481 agcattaatc ccgcctttaa ttgcttttat tcaaaattat ggaattgcat tagaagcaca
    21541 catgcaaaat acagtagtga acttgggccc acattttgac attcaatttt tagtgagaga
    21601 tttaggtggt tctagaattg atttagaaac attacaacat cgtgtatcag atattaaaat
    21661 tacaaatgat agtttaatag ctgattctat agatgcagtg attgcaaaat tccaacatgc
    21721 tgttattcaa aatcaaatgg cagaattaat ccatcatttt aatcagtatg attgtgttga
    21781 agaaaccgaa ttatttaaca tagtacagca agtagtagcg catgccatta acccaacact
    21841 accacatgca aatgagttaa aagatatttt gtttggacca acaattactg tcaaagcgtt
    21901 gttaaatatg agaatggaaa ataaagtaaa gcaatattta aatattgagt tagataatcc
    21961 gataaaaaaa gaggtgtagt actacatggc acacgttaac ataaatatat cgaagattaa
    22021 atataacgcc aaagtacttc aaacagtttt tcaaagtaaa aatatgcaat tcacaccagt
    22081 aattaagtgc atagctggtg accgtacaat tgtagaaagc ttaaaagcgt taggtatcaa
    22141 tcatgttgca gaatccagat tggataacat aattagtatt gcagatcagg atttaacata
    22201 tacgttattg cgtacgcctg ccaaaaaaga gatttcagat atgatagaaa aagttgatat
    22261 gagtatccaa acagaattat ctaccataca tcaaataaat gaagtagcag aagttttagg
    22321 aaaaaaacat aagattttat taatggtaga ttggaaagat ggccgtgaag gtgttcagac
    22381 ttatgatgtt ttagattata ttaaagagat tattcacttg aaaaatatcc attttgtagg
    22441 tttagcattc aattttatgt gttttaaatc tgatgcgcca agtgatgatg atatctttat
    22501 gattaatcga ttcgtctcag ctgttgaaag agaaatagga tatcgattga aaattatttc
    22561 aggtggtaat tcgagcatgt taccacaact tttatataat gatttgggta aaataaatga
    22621 attgcgaata ggtgaaacgt tgtttagagg tgttgataca acgacaaatc aagcgatagc
    22681 tatgttatat caagatgcga ttacattgga ggctgagatt ttagaaatta aacctcgagt
    22741 taatacacaa acgcatgaat cattcttaca agctattgta gatattggtt atttagatac
    22801 aaaagtcgat aatatttcac ctatggatca gcatatcaat attttaggcg cttcgagtga
    22861 tcatttaatg cttgatttaa atggtcaagg acactatcaa gttggagatc atatatcttt
    22921 ttcattaaat tatgaagcac tttctcattc gatgtatatg aaaaatttat ataaggtata
    22981 tattgatgat tcaaagattg atacgctact tcaaaatttt gatgtgaaat ctcccgctat
    23041 ggtaaaccaa tattaatata tttatcaata agtctaagtt gacaagttat actacatatc
    23101 gtttacacgg ttgtatcata attgttcaac ttagattttt tgtatttgtt aatttatcaa
    23161 attaagtgca acagttcgtc acataaaatt gtaacagata atatcagctg aattacaggg
    23221 ataacggtca tgctaaatgg tgtcaattgt attaattcaa agatgatata gccatgatac
    23281 attatcagta ttttgtctaa ggaaatgtgc taattgtagt cataattatt agagggaata
    23341 tatatagtca tacattttag taatataaaa agattgaacg ctacttgaca atgataattg
    23401 ttatcaataa aataataaat aaagttatac atattaagga gtggaacgat gagaggtcta
    23461 aaaacttttg gtatattggg attaatagtt gccttatttt tagttgcagc ttgtggtaat
    23521 acggataatt caagtaaaaa agaatcatca actaaagata ctatttcggt aaaagatgaa
    23581 aatggtacag taaaagtacc taaagatgca aaacgtatcg ttgtattaga gtactcattt
    23641 gcagatgcat tagcagcatt agacgttaaa ccagttggta ttgctgatga tggtaagaaa
    23701 aaacgtatca ttaaaccagt tagagaaaaa attggggatt atacttctgt aggtacacgt
    23761 aaacagccaa acttagagga aattagtaaa ttaaaaccgg atttaattat cgctgatagc
    23821 agtagacata aaggtattaa taaagaatta aacaaaattg caccaacatt atcattaaag
    23881 agttttgatg gagactacaa acaaaacatt aattcgttca aaacaattgc taaagcttta
    23941 aataaagaaa aagaaggcga aaagcgtctt gctgaacatg ataaattaat caaaaagtat
    24001 aaagatgaaa ttaagtttga tagaaatcaa aaagtgcttc cagcagttgt tgctaaagct
    24061 ggtttattag cacatccaaa ctattcatat gttggacaat ttttaaacga acttggattt
    24121 aaaaatgcat taagtgatga tgtaacaaaa ggtttaagta aatatttgaa aggaccttac
    24181 ttacaattag acactgagca tttagctgac gtcaatccag aacgtatgat tatcatgaca
    24241 gatcatgcta aaaaagattc acctgaattc aaaaaattac aagaagatgc aacttggaaa
    24301 aaattaaatg cagttaaaaa taatcgtgtt gatattgtcg accgtgatgt ttgggcaaga
    24361 tctcgtggtt taatttcttc agaagaaatg gctaaagagc ttgttgaatt atcaaaaaaa
    24421 gaacaaaagt aaggtggaag taaatggcta ttaaacaaat tagtagccaa tccgctatag
    24481 attataaaag aaaaaggcgc acaacactca cgtacattgt gagtgtgtgc tttcttttta
    24541 tttgtgttta tttaaatatg gcgattggtt catcaaaaat taactttagc gatatcgtac
    24601 actatctttc tggccatacc gatacaaaag cgtcattttt attacataat gtacgcatgc
    24661 cacgtatgat agcagggcta tttattggtg gtgctttagc agtttcagga ttactaatgc
    24721 aagcgatgac acgtaatcca cttgcatctc caaaaatatt tggtgttagt tctggtgcat
    24781 cattcgttat tgttttagta actattatca ttccttcatt agaatattat gcactatatt
    24841 taggtgtaat tggtgccttt gtaggtggtt taactgtcta cacgctttca ggtgctacaa
    24901 aaggaatgac gccaattaaa ttagctttag ctggtatggc catccatttg ttttttagta
    24961 gtatgactga aggaattatt attttgaatg agaattcaaa cgaacaagtg atgttttggc
    25021 ttgtcggttc attatccagt atgaagttgg atgaaatttt gaccattcta ccatggatta
    25081 ttggtgcttt aattgtaacg atttttattg gacgtcaact aacgataatg gaactaggcg
    25141 atgacattgc taagggattg ggtcaaaata taaataaagt tagaattatc attggactat
    25201 tagtcattat tcttacaggt atgtcggtat cagtagctgg accaatagga tttgttggtt
    25261 taatcgtgcc acatattgta aaacgatacg taagtaagaa ttatttagtg atgattccat
    25321 taacatttat tattggtgcg gatttattac ttctatctga cgttttaagt cgtttaatta
    25381 cgtatcctta tgaatcacca gtaggtattg ttacatcatt tgtaggtgca ctttacttct
    25441 tatttataac aattaaaggg gtgaaaagaa tatgatgaca gccaaaatga tacgtcgtta
    25501 tagtttaatt gtaatccttt taattattag tattttcatc agtttatgcg taggatcagt
    25561 aatgattcat ccgattgatg caataaaagg aatatttacg caagataatt ttattttaaa
    25621 tgagtatcgt attccacgaa cattacttgg aattattata ggtagtagtc ttgcaatatc
    25681 aggtgcgatt atccaagctg taattagaaa cccattagcc tctccagacg tcattggaat
    25741 ttctaaaggt gcaagtttgg cagctgttat cattattatg atttttccaa cagctccatt
    25801 attcgtgtta cctattggct catttgtagg tgcatttgct gtaagtttat ttttatcatt
    25861 tttaatttct aagtatgatg ttaagggttc gaagctggct ttaattggtt tggcaatagg
    25921 cgcaatatgt acggcgattg tgcagttctt attaatcaga aatccactgg aagcaaacaa
    25981 agcgctagtt tggttaacag gtagtttata tggacatgat atgtctaata tttatacgat
    26041 attaccttgg tttttaataa cgataccgat catcatttta ttgagttatc aattagatgt
    26101 attaaactta ggggatgctg ttgcaaccgc actcggttta aaagttaaga caattaaaat
    26161 gctgttatta attttagcag tcgtattagc gggatctgca atatctgtag taggtggtat
    26221 tagcttttta ggtttaatcg caccacacat agcaaggcaa ctaataggta ataaaacatt
    26281 acatgtcatc atcatgtcag ggttaattgg tgctatattg ttaacatttg gagatggttt
    26341 agcccgtggc attcatccgc cactggatat tcctgtaggg gttatcattg caattatcgg
    26401 tgcaccatac ttcttaatat tattacgaag aatgaaataa taaatatcgt tttttaaatt
    26461 taaaggttaa gtacgaatgt tgaaattgta cttaaccttt tttagaattc accttgaaaa
    26521 aacagcaaga atagcgtata tttaattact gtaattattc attattttgc atacataaac
    26581 attttggata ttggaatgat atgaaatatg attgttaaat gaatagaatg ttgtatatac
    26641 ttaacacgaa tacatagaga aacggattga ttttatggat agcttttcaa ggaaagagat
    26701 agtcattggg agacttaaat ttatcacgat gtctttaata ggtattttgt tatttctaat
    26761 acctataccg gttgaacaag atggtcaaaa gcaaacaact ttaccagttg catttttagc
    26821 aggtgtactt aaagatgtat taggcgatgt tatgccaata ttaattgtga cgataattac
    26881 tttatcaggt attttaacgt tgatttgttc tactatttta aaagataaat tgaaacctga
    26941 tggattaatg aataatgctt ttaacgtaag aattggttgg ttaattttac gtatattagc
    27001 agtgatattt gcatggatga catttttacg tattggatca aaagtaatat attcagatga
    27061 aacaggtgga ttactatttt ctagtttatt gccaacatta gttgcggtat tcttatttgc
    27121 agcattattt ttacctttat taatggaata tggattacta gagatgttag ggccaatttt
    27181 cagacctatt atgagacctt tatttacatt gccaggacgt tcgacggtag ataatttagc
    27241 atcatttatt ggagacggaa ctgttggtgt cttaattaca agtagacaat atggtgaagg
    27301 atattattct cgtcgtgagg caacagttat ttcaacgaca tttagtgtag tttctatcac
    27361 ctttgcaatt gtagttgctg aaacagtgca catgcaaaat caattttttg ctttctattt
    27421 atctgttatc gtatcttgtc ttgttgcagc tgtcattatg ccaagaattt ggccattgaa
    27481 caaaattcct gatgagtatg ctaaggaggt acctgaaagt gcgcgtactg aggcattgcc
    27541 agaaggaaag acggcattga gacatggctt tgatactgca acagaagttg gtattaaagc
    27601 accgggcgtt attgactttt ttaagtcagg attaaaaaca gtcattgata tgtggtttgt
    27661 tattttgcct gtagtgatga gtattggtac tattgctacc attattgcga actacacgcc
    27721 cttttttgtc atattgggta aaccttttgt tccattttta gagttaatgc aaatacctga
    27781 agcggcacag gcttctcaaa cgattattat cggttttgcc gatatgtttt taccttctat
    27841 attaattgaa ggtgtacaaa atgatataac tcggtttgtt attggggcac ttagtatatc
    27901 gcagttaata tacttgtctg aagtaggtgg cgtcatactt ggttcgaaga ttcctgtaag
    27961 catcggcaaa ttgtttatga ttttcttgat tagaacaatc attacactac caattatttc
    28021 tttgatggca catcttttgt tataaatata tgtgaaaaag gacaactgtt aaaattatgg
    28081 gaacagttgt ccttttgtat ttgtttatgc tgggtgttga atcaattaaa aaaatcattc
    28141 atgaagccct caccatgaat gattttttta tacaatttat ttagttaatt cattgtaaac
    28201 tttttgatca ttttgagtat aaataatgta ttcgtggtct tcagtatcga taataacgcg
    28261 attcgtttta ccaaatgttg agccgattct agatacatct ttgctgtcta atttaggaac
    28321 tgcatgaata tcttggtctt gtgtaatatt tttaatttca cttgtaggaa ttttgatatc
    28381 agcaactctc cattgaatac gcacttcatt attatctttc tttactgtca ttgccattat
    28441 tcaaacacat cctttaaata ttttgtaaat acatcataaa cgattttgta atcgttttca
    28501 agagttatgt gatacttttt taaattgtgt catactaatg atttttaacc cgaaaatatg
    28561 ttcgtgtttt tgttagggat atggtaaaat gtgagtatta aaggagaaca aatatgacag
    28621 gtaaaacaca tgcttcatgc ggcatgctcg ttggagcgct aacaacacaa tattttcaaa
    28681 cagatatttt ttcgtcggtt acggttatca ttctagcaac tctagctagc ttattacccg
    28741 atatatgtca cactcaaagt aagattggca gaaggtttaa ggtaattagt ttttttgtga
    28801 gattgatctt tggacatcga acctttacgc attcaatatt atttatcgca atcatagcat
    28861 ttttattgca aattattcag acgcctaatt attatatggc agcaattata attggattag
    28921 tgtcacatgt tatactagat atgataacac caaaaggcgt gaaattattt tatccgttac
    28981 cgtttaatgt gaagacacca atccaattta aaacaggtgg acttgtagac ttatctttag
    29041 ctactgcgtt aatggtcgga acagtttatg ttttatttca gcctttcatc aatgatataa
    29101 taagcaactg gaatactaag tttttataga aaataaatta ttaaaggtta caaactcagc
    29161 aggaggcata aaatgctaga taaaaatcaa ttagctaaat ataaacaaga tcatttgtgt
    29221 gaatatgaaa aaataatgag taacaatgaa aaagaagcac ttgaagagaa ggtagcgtca
    29281 ttagatttag attttattgc caaattatat aacgatttat acatcaataa gaaaacaata
    29341 gatgatgtat ctgcagtttc tgaagtgaaa tatgatatta aatcacaaat gagtgatgat
    29401 gagattaagc gtttagagga acaaggactt caagctatta aagaaggaca atttgcagta
    29461 cttttgatgg caggtggtca aggaacaaga cttggttaca agggccctaa aggttcgttt
    29521 gaaattgaag gtgtaagttt atttgaactt caagctaacc aattaaaaac attgaatcat
    29581 caatctggac atacgattca atggtatatt atgacaagtg atatcaatca tgaagaaact
    29641 ttagcttatt tcgaagcaca tagttatttt ggatatgatc aagaagcaat ccatttcttt
    29701 aaacaagata atattgtggc gcttagtgaa gaagggaagt taattttaaa tcaacagggt
    29761 cgtattatgg aaacgccgaa tggtaatggt ggcgtgttta aatctttaga caaggcagga
    29821 tacttagaag aaatgtctaa taatggcgtt aagtatattt tcttgaataa tatcgacaat
    29881 gttttagtaa aagtgttgga tccactattt gcaggattta cggtagaaca tgattacgat
    29941 attacttcta aaacgattca accaaaacca ggagaaagtg ttggacgttt agttaatgtc
    30001 gattgtaaag atacagtact ggagtattca gaattagatc cagaagttgc aaatcaattt
    30061 aacaatgcta atatcggtat tcatgcattt aaacttggtt ttattttaaa cgctgtaaat
    30121 cgtgagttac cttatcattt agctattaag aatttgaaac aattagatga gaactttggt
    30181 gttattgaac aaccaacttt aaaattcgaa ttgttctatt ttgacatatt tacttatgga
    30241 actagttttg tcacgttaca agttcctaga gaagaagaat tttcacctct taaaaataaa
    30301 gaaggtaaag atagtgttgc aactgcaaca gaagatttac gtagaatggg tttaatttaa
    30361 tgataggcgg tgactatgat gtccaaatca actaaaaaaa gtaatgacac aatcgtagaa
    30421 acatttaagg atatcatccc tttatcattt ggagaagaaa ttggtaatgc ggcatctcat
    30481 gggctggcag ctttgttaac attattagtg ttaccctatg cggctgtgca cagttatatt
    30541 gcacatggta cgcttgcgtc tgtgagtatg tctatttatg ttatttctat ctttatgatg
    30601 tttatttcat ctactattta tcattctatg caaaatgaaa cacctcataa atatatttta
    30661 aggattatcg atcacagtat gatttacgta gcaatttctg gtacatatac gcctattttg
    30721 ttaacagttg taggtggttg gataggttgg acagtattta ttttattatg gggaacgaca
    30781 ctttggggta ttttatataa atccatagct gtcaaagtta accaaaagtt aagcttaatt
    30841 gtctatttag tgatgggctg ggtaggtatt atcttgttac caattatttt tattcgcaca
    30901 tcatggatat tcatattatt cattttctta ggtggtgtgt cttatacaat tggtgcatgg
    30961 ttttatgcac aaaagaatag accatatttt catatgattt ggcatatttt tattgtaata
    31021 gcatcactat tgcatttaat tgcaatctta tattttatgt agtcgtttag aaaaggtggg
    31081 ggaaactccg ctttttttat tacttaaatc attctattag gggattgtat tgtaataaaa
    31141 ttatcgttaa gctttgaaag tcaaaagtgt ttatacatat aaaaatcaat agtttgtaaa
    31201 taaaatctta taaatatata gaaggaggta tttcatgcga ttaaagtcaa tcataactgt
    31261 tatagcttta atattaatta tgtttatgtc tgcgatagaa tcatctatta tttcgcttgc
    31321 attacctaca attaaacaag atttaaatgc aggaaattta atttcattaa tttttaccgc
    31381 gtattttatc gcattagtaa ttgcaaatcc tatagtgggc gaattacttt cgaggtttaa
    31441 aattatttat gtggcaattg caggattact tttatttagt ataggtagtt ttatgtgtgg
    31501 tttaagtact aattttacta tgctaattat atcacgcgtg atacaaggtt ttggatcagg
    31561 tgtattgatg tcattatcgc aaattgttcc aaagttagca tttgaaattc cgttacgata
    31621 taaaattatg ggcatagttg gcagtgtttg gggtatttcg agtatcattg ggccacttct
    31681 aggtggtggt atattagaat ttgcaacatg gcattggtta ttctacataa atataccgat
    31741 tgcgatcatt gccattatat tagttatatg gacatttcat tttccagagg aagaaacagt
    31801 tgctaaatca aaatttgata ctaaagggct cacgttattt tatgtattta ttggattgat
    31861 tatgtttgct ttattaaacc aacagctatt acttttaaat ttcttaagtt ttatcttagc
    31921 tatcgttgtg gcaatacgtt tatttaaagt agaaaaacat gtttcttcac catttttacc
    31981 tgtggttgaa tttaatcgtt cgattacttt agtttttata actgaccttc taacagctat
    32041 ttgtttaatg ggattcaatt tatatattcc agtctacctt caagaacaac taggattatc
    32101 tccattacaa agtgggttgg ttatttttcc tttatctgta gcttggatta cattgaattt
    32161 taatttacat cgaattgaag caaaactatc aaggaaagtt atttacttac tatcatttac
    32221 attgctacta gtaagtagta ttatcatttc atttggtatt aaattgccgt tacttattgc
    32281 atttgtatta attttggcag gattaagttt tggatatatt tatacgaaag atagtgtaat
    32341 tgtccaagaa gaaactagcc cattacaaat gaaaaaaatg atgtcatttt atggattaac
    32401 taaaaatctt ggggcatcaa taggttcaac aataatggga tatctttatg cgatacaatc
    32461 aggaatcttt ggtccaaact tacacaatgt gttaagtgct gttgctgtaa ttagcattgg
    32521 gcttattgtt ttatgggtcg tattttttaa agaacagtca tctcaatcaa aagaatagaa
    32581 ttatgattaa aattgatgta gtgtaattaa agtaagaaat aataaattta ctagatgcta
    32641 gataaatcgt tcaatatttt attaataagg aggccaatga ttgtgaatta tcttaaacat
    32701 aagttttata acttgttaac tacaatgatt gttctcttta tttttgtact ttctggtgcg
    32761 atttttttaa catttctagg gtttggttta tatggattaa gtagaatact tattcatttt
    32821 aggttgggtg actttacata taatagaagt atgtacgata acctattgta ttatggcagt
    32881 tatataatct ttgggtactt catcatattt gcagtcgagc atttaatgga ttactttaga
    32941 aaaatgttgc ctgaaaatgc atattttaga ggcgcgactt ttcatttgat ttcttacaca
    33001 gttgcaacaa cgttatttta tttcatcatt catttaaatt atgtttatat caatatcgat
    33061 ttttgggtaa tcatggttat tatagggttc ttatatgttt gtaagcttca attttaccct
    33121 gaaagtaaaa atttaaataa tagaaaatag atatctgaag ttgagtgcac atatgtgttc
    33181 caacttcttt ttgttcaata gaagaatcga taatagtgag tcttagtgct caatttgtgg
    33241 taaataagtt aaataaccat tgtatgagtc tgagacataa aataagttat taggcgctac
    33301 aatgttatat tgttagtttt ttaagtcagg ctgggacata aatcaatgtt ctatgctcta
    33361 cgaagttata ttggcagtag ttgactgaac gaaaatgcgc ttgtaacaag cttttttcaa
    33421 ttctagtcaa ccttgccggg gtgggacgac gaaataaatt ttgcgaaaat atcatttctg
    33481 tcccactccc aagaaccaat aatgatttat tatgtagtgc ttctttatca tttgagtact
    33541 actcccatta tgttaatggc tatatgtatt aaagggacaa gtgtatatct gtttaaaaag
    33601 gaggaattat gatggctaaa gttgaattaa caacccggag acgaaacttc atagttgctg
    33661 ttatgttgat tagtgcgttt gtagctattt taaatcaaac attattaaat acagcgttac
    33721 ctagtataat gagagaatta aatattaatg aaagtacatc gcaatggcta gttactgggt
    33781 ttatgcttgt taatggcgtc atgatacctc tgacggcata tctaatggat aggattaaaa
    33841 ctagaccttt atacttagca gcgatgggga catttttatt aggttctatt gttgcagcct
    33901 tagctccgaa ttttggagtt ttaatgttag cacgtgtaat tcaagcgatg ggtgcagggg
    33961 tacttatgcc cttaatgcaa tttacgttat ttacattgtt cagtaaagaa catcgaggtt
    34021 ttgcaatggg gctggcaggt ttagtaattc aatttgcacc agcaatagga cctacagtta
    34081 caggattaat cattgatcaa gcgagttggc gagtaccatt tattataatt gtaggaattg
    34141 ctttagttgc ctttgttttc ggtttggttt caatctcgag ttacaatgaa gtgaaatata
    34201 ctaaattaga taagcgctca gtaatgtatt caactattgg gttcgggtta atgttatatg
    34261 cattcagtag tgcaggcgac ctaggattta ctagcccggt agtcattggt gcaatgataa
    34321 ttagttctat tatcatctac ttatttattc gtagacaatt caatataagc aatgcacttt
    34381 taaatttaag ggtttttaaa aatagaacat ttgcattatg tacgattagt tcaatgatta
    34441 ttatgatgtc aatggttgga cccgcgctgc ttataccgct atatgttcaa aacagtttat
    34501 ctttatctgc cttgttatca ggactagtta tcatgcctgg tgcaataata aatggtatta
    34561 tgtcggtttt tacaggtaaa ttttatgata agtatggtcc taggccattg atttatactg
    34621 gttttactat tttaacaatt actacaatta tgttgtgttt cttgcataca gacacatctt
    34681 atacgtattt aatagttgta tatgcaatta gaatgttttc agtttcttta ctcatgatgc
    34741 cgataaatac tacaggaatt aattctttga gaaatgaaga aatctcacat ggcacggcta
    34801 ttatgaactt tggtcgtgta atggctggtt cactaggtac agctttaatg gttacattaa
    34861 tgagttttgg tgcaaaaata tttttatcaa cttcaccatc acatttaact tcaaatgaga
    34921 taaaacaaca atcaattgct attggagtag atatctcatt tggatttgta gcagttctag
    34981 ttatgattgc ttatgtgata gcaattttta tcagagaacc tgaagagatt gaaagtaata
    35041 gaaggaaatt ttaaaataat atgagtagtt ggtctattga aaataatagg ctaacttctt
    35101 tttttattta ataaaaagtt ttatactttt agtgatagac taagcaaaaa ttgttatttg
    35161 ctatgatgta gatgtcttaa aatgattaag ggggatttgc tttgttaacg gtagatcaag
    35221 tgaaagaatt ggtaggagaa attaaagatc ctattataga tgtgccttta aaagaaacag
    35281 aaggtattgt tgaagtttct attaaggaag aaaaagaaca tgtgagtgtt aaattagcaa
    35341 tggcacaatt aggtggtgca ccgcaattag atttacagat ggcagttgtt aatgctttaa
    35401 aagaaaatgg tgcaaaaact gtgggtatac gatttgaaac gttaccagaa gaaaaagtaa
    35461 atcaatttaa accaaaagaa gaaaataaac ctaaaacgat agaagggctg ttatctcaaa
    35521 ataatccagt tgaatttatt gctattgctt caggtaaagg tggcgtcggt aaatctactg
    35581 ttgcagtaaa tttagccgtt gccttagccc gtgaaggtaa aaaagttgga ttagtagatg
    35641 ccgatatata tggatttagt gtaccagata tgatgggtat tgatgaaaaa cctggaatta
    35701 aagggaagga agtaattcca gttgaacgtc atggcgttaa agttatatca atggcctttt
    35761 ttgtggaaga aaatgcgcca gttatatgga gagggccaat gttaggtaaa atgttgacga
    35821 atttctttac agaagttaaa tggggagaca ttgaatattt aatactcgat cttccacctg
    35881 gaacaggaga tgtagcttta gatgttcata cgatgttacc ttcaagtaag gaaattattg
    35941 taacgacacc tcatcctaca gcagcatttg ttgcagctcg cgcaggtgcg atggcaaaac
    36001 atacggatca ttctattctt ggcgtagttg aaaacatgag ttattttgaa agtaaagaga
    36061 cgggtaataa agaatatgtc tttggcaaag gtggcggtac taagttagct gatgaactta
    36121 atactcaatt acttggggaa ttacctttag agcaaccatc ttggaatcca aaagattttg
    36181 caccgtcgat atatcaatca gatgatcgtc taggtaaaat ttatagctcg attgcacaaa
    36241 aagttatagc tgctacaaat aaataggtat aatatatgct aaacgtacaa aattactttt
    36301 attttgtacg tttttatatt gaattgtttt agaatgctgg taaaataaat tcttgtgtta
    36361 agcaatcaac gcattgaaaa aagaagcggt ttgcaaacac gaatgttttt caaaaaagtt
    36421 attgacttta aattataaag ttgataagat aataaagtcg tcaaaaacgg cgacaatgtt
    36481 tttaaagaag aatttcaaaa gtgtaaaact taccattgaa ataactataa aaacaatata
    36541 gaataaataa agtaatcgtt caaaaagtta ttgacttcaa caattaaacg aagtataata
    36601 aaagagattg aacattaatg aacattgaaa actgaatgac aatatgtcaa cgttaattc
//
LOCUS       DAHHRR010000013        36030 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.013, whole genome shotgun sequence.
ACCESSION   DAHHRR010000013 DAHHRR010000000
VERSION     DAHHRR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36030
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.013"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            124..1020
                     /gene="xerC"
                     /locus_tag="NBI42_000550"
     CDS             124..1020
                     /gene="xerC"
                     /locus_tag="NBI42_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCD1776995.1"
                     /translation="MNHIQEAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLQL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKSLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCKHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            1017..1562
                     /gene="hslV"
                     /locus_tag="NBI42_000551"
     CDS             1017..1562
                     /gene="hslV"
                     /locus_tag="NBI42_000551"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCD1776996.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            1628..3031
                     /gene="hslU"
                     /locus_tag="NBI42_000552"
     CDS             1628..3031
                     /gene="hslU"
                     /locus_tag="NBI42_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCD1776997.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            3056..3829
                     /gene="codY"
                     /locus_tag="NBI42_000553"
     CDS             3056..3829
                     /gene="codY"
                     /locus_tag="NBI42_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCD1776998.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFVDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            3903..3977
                     /locus_tag="NBI42_000554"
     CDS             3903..3977
                     /locus_tag="NBI42_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1776999.1"
                     /translation="MTLMIMLKSFLYFCLIFNQIDIPH"
     gene            4176..4943
                     /gene="rpsB"
                     /locus_tag="NBI42_000555"
     CDS             4176..4943
                     /gene="rpsB"
                     /locus_tag="NBI42_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCD1777000.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            4977..5090
                     /locus_tag="NBI42_000556"
     CDS             4977..5090
                     /locus_tag="NBI42_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777001.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            5125..6006
                     /locus_tag="NBI42_000557"
     CDS             5125..6006
                     /locus_tag="NBI42_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCD1777002.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            6143..6865
                     /locus_tag="NBI42_000558"
     CDS             6143..6865
                     /locus_tag="NBI42_000558"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCD1777003.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            6884..7438
                     /gene="frr"
                     /locus_tag="NBI42_000559"
     CDS             6884..7438
                     /gene="frr"
                     /locus_tag="NBI42_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCD1777004.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            7811..8581
                     /locus_tag="NBI42_000560"
     CDS             7811..8581
                     /locus_tag="NBI42_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCD1777005.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            8588..9370
                     /locus_tag="NBI42_000561"
     CDS             8588..9370
                     /locus_tag="NBI42_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCD1777006.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            9582..10868
                     /gene="rseP"
                     /locus_tag="NBI42_000562"
     CDS             9582..10868
                     /gene="rseP"
                     /locus_tag="NBI42_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCD1777007.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTERKLTK
                     VSSETKYVLGFQPASERTLFKPIVYGFESFLKGSTLIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            10888..12591
                     /locus_tag="NBI42_000563"
     CDS             10888..12591
                     /locus_tag="NBI42_000563"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCD1777008.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVSPFDVHLISINPKKDDQRELADQLYAKFNEKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            12855..17165
                     /locus_tag="NBI42_000564"
     CDS             12855..17165
                     /locus_tag="NBI42_000564"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCD1777009.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFIHAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            17455..17922
                     /gene="rimP"
                     /locus_tag="NBI42_000565"
     CDS             17455..17922
                     /gene="rimP"
                     /locus_tag="NBI42_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCD1777010.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            17943..19118
                     /gene="nusA"
                     /locus_tag="NBI42_000566"
     CDS             17943..19118
                     /gene="nusA"
                     /locus_tag="NBI42_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCD1777011.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVTDVSVETNVEKESE"
     gene            19139..19423
                     /locus_tag="NBI42_000567"
     CDS             19139..19423
                     /locus_tag="NBI42_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCD1777012.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            19420..19737
                     /locus_tag="NBI42_000568"
     CDS             19420..19737
                     /locus_tag="NBI42_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HCD1777013.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            19742..21850
                     /gene="infB"
                     /locus_tag="NBI42_000569"
     CDS             19742..21850
                     /gene="infB"
                     /locus_tag="NBI42_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCD1777014.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSSNQNQNKGQQKDNKKNQQQNNKGNKKNNRNNKK
                     NNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVANINQ
                     SLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHVDHG
                     KTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARGAQV
                     TDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTEYGL
                     IPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAELDKS
                     RGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGINDVP
                     QAGDRFVVFSDEKQARRIGESRHEASIVQQRQESKNVSLDNLFEQMKQGEMKDLNVII
                     KGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNVRPD
                     SGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKVSKV
                     GTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGITIEN
                     YNDLKEGDVIEAFEMVEIKR"
     gene            22236..22586
                     /gene="rbfA"
                     /locus_tag="NBI42_000570"
     CDS             22236..22586
                     /gene="rbfA"
                     /locus_tag="NBI42_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCD1777015.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            22756..23673
                     /gene="truB"
                     /locus_tag="NBI42_000571"
     CDS             22756..23673
                     /gene="truB"
                     /locus_tag="NBI42_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCD1777016.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLEMKGVHSADFNNDDI
                     DRLLENFKGVIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            23688..24659
                     /gene="ribF"
                     /locus_tag="NBI42_000572"
     CDS             23688..24659
                     /gene="ribF"
                     /locus_tag="NBI42_000572"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCD1777017.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            24774..25043
                     /gene="rpsO"
                     /locus_tag="NBI42_000573"
     CDS             24774..25043
                     /gene="rpsO"
                     /locus_tag="NBI42_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCD1777018.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
     gene            25413..27509
                     /gene="pnp"
                     /locus_tag="NBI42_000574"
     CDS             25413..27509
                     /gene="pnp"
                     /locus_tag="NBI42_000574"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCD1777019.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDKIEVKITE
                     IDKQGRVNASHRALEE"
     gene            27745..29418
                     /locus_tag="NBI42_000575"
     CDS             27745..29418
                     /locus_tag="NBI42_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCD1777020.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            29675..32044
                     /locus_tag="NBI42_000576"
     CDS             29675..32044
                     /locus_tag="NBI42_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCD1777021.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHRDVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNERPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQKEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            32049..32762
                     /locus_tag="NBI42_000577"
     CDS             32049..32762
                     /locus_tag="NBI42_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1777022.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNELDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            32793..34058
                     /locus_tag="NBI42_000578"
     CDS             32793..34058
                     /locus_tag="NBI42_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD1777023.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVKKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKERFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            34058..35344
                     /locus_tag="NBI42_000579"
     CDS             34058..35344
                     /locus_tag="NBI42_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD1777024.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFNTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVNPEEICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            35344..>36030
                     /locus_tag="NBI42_000580"
     CDS             35344..>36030
                     /locus_tag="NBI42_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD1777025.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSDNGRIIVISSIWGETGASMETIYSTMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMINELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVN"
BASE COUNT        13226 a         4931 c         7046 g        10827 t
ORIGIN      
        1 aattaacagg ttttccgatt agttacaatt atttaattct caaaagattt agaattgatt
       61 atcaaattac tgtaagccct ttgctgtata tgctacaatt cttattgatg gagggtaaat
      121 gtattgaatc atattcaaga agcgttttta aatacattga aagttgaacg gaatttttcg
      181 gaacacacat tgaaatcata tcaagatgac ttaattcagt ttaatcaatt tttagaacaa
      241 gaacatttac agttgaatac ttttgaatac agagatgcta gaaattattt gagctattta
      301 tattcaaatc atttgaaaag aacatctgtt tctcgtaaaa tctcaacgtt aagaactttc
      361 tatgaatatt ggatgacgct tgatgagaac attattaatc catttgttca attagtacat
      421 ccgaaaaaag aaaaatatct tccgcaattc ttttatgaag aagaaatgga agcgttattt
      481 aaaactgtag aagaggacac ttcaaaaagt ttacgggatc gagttattct tgaattgtta
      541 tatgctacag gcatccgtgt ttcggaatta gttaatatta aaaaacaaga tatagatttt
      601 tacgcgaatg gtgttaccgt attaggaaaa gggagcaaag agcgctttgt accgtttggt
      661 gcttattgta gacaaagcat cgaaaattat ttagaacatt tcaaaccaat tcagtcatgc
      721 aaacatgatt ttcttattgt aaatatgaag ggtgaagcaa tcactgaacg cggtgtacga
      781 tatgttttaa atgatattgt taaacgaaca gcaggcgtaa gtgagattca tccccacaag
      841 ctcagacata catttgcaac gcatttattg aatcaaggtg cagacctaag aacagtacaa
      901 tcgttattag gtcatgttaa tttgtcaaca actggtaaat atacacacgt atctaaccaa
      961 caattaagaa aagtgtatct aaatgcacat cctcgagcga aaaaggagaa tgaaacatga
     1021 gtaatacaac attacatgca acaacaattt atgctgtaag acataatggg aaagcagcta
     1081 tggctggaga tgggcaagta acgcttggtc aacaagtcat catgaaacaa acggcaagaa
     1141 aagtgcgacg tttatatgaa ggtaaagtgt tagctggttt cgcaggtagt gtagcagatg
     1201 cgtttacgtt atttgaaaaa ttcgaaacaa aattacaaca gtttagtggt aacttagaaa
     1261 gagctgctgt tgaattggca caagaatggc gaggcgataa acaattacgt caattagaag
     1321 ctatgctaat tgtaatggat aaagatgcta ttttagttgt cagtggaact ggcgaagtta
     1381 ttgccccaga tgatgacctt atcgctattg gatcgggagg caactacgca ttaagcgcag
     1441 gacgtgcatt gaagcgccat gcatcgcatt tgtctgctga agaaatggca tacgagagct
     1501 tgaaagtagc ggctgatatt tgtgtcttta ccaacgataa tattgttgtc gaaacactat
     1561 aataatcaga gcacgataaa taattacgag caattaattt tagttaaaag acggaggaat
     1621 gaaattaatg gatacagctg gaataagatt aactccaaaa gaaatcgtat ctaaattaaa
     1681 tgaatacatc gttggacaaa atgatgctaa acgtaaagtg gcaattgccc tacgtaatcg
     1741 atacagaaga agtttattag atgaggaatc aaagcaagaa atttcaccta aaaatatttt
     1801 gatgattgga ccaaccggcg ttggtaaaac tgaaattgca agaagaatgg ccaaagttgt
     1861 cggcgcgcca tttataaaag tagaagctac taaatttact gaagtaggtt atgtaggacg
     1921 agatgttgaa agtatggtta gagatcttgt tgatgtttca gtaagattag tcaaggcgca
     1981 gaaaaaatca ttggtgcaag atgaagcaac agctaaggcc aatgaaaaac ttgttaagtt
     2041 attagttcca agtatgaaaa agaaagcatc tcaaacgaat aatcctttag agtcactttt
     2101 cggaggtgca attccaaatt tcggacaaaa taacgaagat gaagaagaac cacctactga
     2161 ggaaattaaa acaaaacgtt ctgaaattaa gagacaatta gaagaaggca aacttgaaaa
     2221 agaaaaggta agaattaaag tcgaacaaga tcctggtgct ttaggtatgc taggtacaaa
     2281 tcaaaatcag caaatgcaag agatgatgaa tcaattaatg cctaaaaaga aagttgagcg
     2341 agaagttgct gttgagacgg caaggaaaat cttagctgat agttatgcgg atgaactaat
     2401 tgatcaagaa agcgctaacc aagaagcgct tgaattagca gaacaaatgg gtatcatctt
     2461 tatagatgaa atcgacaaag ttgcgacgaa taatcataat agtggtcaag atgtctcaag
     2521 acaaggtgtt caaagagata ttttacctat acttgaaggt agcgttattc aaaccaaata
     2581 tggtactgtg aatactgaac atatgctgtt tataggtgct ggagctttcc atgtatctaa
     2641 gccgagtgac ttgataccag aattgcaagg tcgttttccg attagagttg aacttgatag
     2701 tttatcggta gaagattttg taagaatttt gacagaacca aaattgtcat taattaaaca
     2761 atatgaagca ttgcttcaaa cagaagaagt tactgtaaac tttaccgatg aagcaattac
     2821 tcgcttagct gagattgctt atcaagtaaa tcaagataca gacaacattg gtgcacgtcg
     2881 acttcataca attttagaaa agatgttaga agatttatca ttcgaagcac caagtatgcc
     2941 gaatgcagtt gtagatatta ccccacaata tgttgatgat aaattaaaat caatttcaac
     3001 aaataaagat ttaagtgcat ttattctata aaaatataca aaaggagaaa aattcatgag
     3061 cttattatct aaaacgagag agttaaacac gttacttcaa aaacacaaag gtattgcggt
     3121 tgattttaaa gatgtagcac aaacgattag tagcgtaact gtaacaaatg tatttattgt
     3181 atcacgtcga ggtaaaattt tagggtcgag tctaaatgaa ttattaaaaa gtcaaagaat
     3241 tattcaaatg ttggaagaaa gacatattcc aagtgaatat acagaacgat taatggaagt
     3301 taaacaaaca gaatcaaata ttgatatcga caatgtatta acagttttcc cacctgaaaa
     3361 cagagaatta ttcgtagata gtcgtacaac tatcttccca attttaggtg gaggagaaag
     3421 attaggtaca ttagtacttg gtcgagtaca tgatgatttt aatgaaaatg atttggtact
     3481 aggtgaatat gctgctacag ttattggtat ggaaatctta cgtgagaagc atagtgaagt
     3541 agaaaaagaa gcgcgcgata aagctgctat tacaatggcg attaattcat tatcttattc
     3601 tgaaaaagaa gcaattgaac atatctttga agaacttggc ggtacggaag gcctattaat
     3661 cgcatcaaaa gttgcagata gagttggtat tactagatct gtaattgtaa atgcactacg
     3721 taaattagaa agtgctggtg taattgaatc acgttcttta ggaatgaaag gtactttcat
     3781 taaagttaaa aaagaaaaat tcttagatga attagaaaaa agtaagtaaa tcgatgagtc
     3841 tgggagataa ttcaatgtcc tagtcgtgac gatgttatct tggcagtagt caactcaacg
     3901 aaatgacttt gatgattatg ttgaagtcat ttctttattt ttgtctaatt ttcaatcaaa
     3961 ttgatattcc acattaacat gatagaatta cctttaaatt tatgaaaaaa tgcttgaaaa
     4021 acatttgctt tagacacggc attatgttat atttattttc ggctataaag ccaatacaca
     4081 catatcaaac gtgattgtgt aaagggtgca atatttgtat attgtcttta caatagtttg
     4141 ctatggaggt aattaaccaa taggaggaat ttataatggc agtaatttca atgaaacaat
     4201 tactagaagc gggtgttcac ttcggtcacc aaacacgtcg ttggaaccca aaaatgaaaa
     4261 aatatatctt cactgagaga aatggtattt atatcatcga cttacaaaaa acagtgaaaa
     4321 aagtagacga ggcatacaac ttcttgaaac aagtttcaga agatggtgga caagtcttat
     4381 tcgtaggaac taaaaaacaa gcacaggaat cagttaaatc tgaagcagaa cgtgctggtc
     4441 aattctacat taaccaaaga tggttaggtg gattattaac taactataaa acgatctcaa
     4501 aacgaatcaa acgtatttct gaaattgaaa aaatggaaga agatggttta ttcgaagtat
     4561 tacctaaaaa agaagtagta gaacttaaaa aagaatacga ccgtttaatc aaattcttag
     4621 gcggaattcg tgatatgaaa tcaatgcctc aagcattatt cgtagttgac ccacgtaaag
     4681 agcgtaatgc aattgctgaa gctcgtaaat taaatattcc tattgtaggt atcgttgaca
     4741 ctaactgtga tcctgacgaa attgactacg ttatcccagc aaacgacgat gctatccgtg
     4801 cggttaaatt attgactgct aaaatggcag atgcaatctt agaaggtcaa caaggcgttt
     4861 ctaatgaaga agtagctgca gaacaaaaca tcgatttaga tgaaaaagaa aaatcagaag
     4921 aaacagaagc aactgaagaa taatcaactg ttgaatctga cttagatata gtttaaatgg
     4981 gtgataagat attaatgctt atcacctttt ttaaaaagaa aatcgaggca aattacaaat
     5041 attcaattag agtattggca atcttgccta taataatgct aaaatcataa tatataaaat
     5101 gataacttat tggaggaata atgaatggca actatttcag caaaacttgt taaagaatta
     5161 cgtgaaaaaa ctggcgcggg tatgatggat tgtaaaaaag cgctaactga aactgatggt
     5221 gacatcgata aagcgattga ttacctacgt gaaaaaggta ttgctaaagc agctaaaaaa
     5281 gcagaccgta ttgcggctga aggtttagta catgtagaaa ctaaaggtaa cgacgcagtt
     5341 atcgttgaaa tcaactctga aacagacttt gttgctcgta acgaaggttt ccaagagtta
     5401 gttaaagaaa tcgctaatca agtattagat acaaaagctg aaactgttga agctttaatg
     5461 gaaacaactt taccaaatgg taaatcagtt gatgaaagaa ttaaagaagc aatttcaaca
     5521 atcggtgaaa aattaagtgt tcgtcgtttt gctatcagaa ctaaaactga taacgatgct
     5581 ttcggcgctt acttacacat gggtggacgc attggtgtat taacagttgt tgaaggttca
     5641 actgacgaag aggcagcaag agacgttgct atgcatatcg ctgcaatcaa ccctaaatat
     5701 gtttcttctg aacaagttag cgaagaagaa atcaaccacg aaagagaagt tttaaaacaa
     5761 caagcattaa atgaaggtaa accagaaaac atcgttgaaa aaatggtgga aggacgttta
     5821 cgtaaatact tacaagaaat ttgtgctgta gatcaagact tcgttaaaaa ccctgatgta
     5881 acagttgaag ctttcttaaa aacaaaaggt ggaaaacttg ttgacttcgt acgctatgaa
     5941 gtaggcgaag gcatggaaaa acgcgaagaa aactttgcgg atgaagttaa aggacaaatg
     6001 aaataatctg tcataaagta aaacaaggaa gaagacacct ttaatgttgc tttattaaaa
     6061 tgtaaatcat tctaataaaa cgacaactgt gtcttcttta cttgtatatg ttacatatat
     6121 tcacgataga gaggataaga aaatggctca aatttctaaa tataaacgtg tagttttgaa
     6181 actaagtggt gaagcgttag ctggagaaaa aggatttggc ataaatccag taattattaa
     6241 aagtgttgct gagcaagtgg ctgaagttgc taaaatggac tgtgaaatcg ctgtaatcgt
     6301 tggtggcgga aacatttgga gaggtaaaac aggtagtgac ttaggtatgg accgtggaac
     6361 tgctgattac atgggtatgc ttgcaactgt aatgaatgcc ttagcattac aagatagttt
     6421 agaacaattg gattgtgata cacgagtatt aacatctatt gaaatgaagc aagtggctga
     6481 accttatatt cgtcgtcgtg caattagaca cttagaaaag aaacgtgtag ttatttttgc
     6541 tgcaggcatt ggaaacccat acttttctac agatactaca gcggcattac gtgctgcaga
     6601 agttgaagca gatgttattt taatgggcaa aaataatgta gatggtgtat attctgcaga
     6661 tcctaaagta aacaaagatg cggtaaaata tgaacattta acgcatattc aaatgcttca
     6721 agaaggttta caagtaatgg attcaacagc atcctcattc tgtatggata ataacattcc
     6781 gttaactgtt ttctctatta tggaagaagg aaatattaaa cgtgctgtta tgggtgaaaa
     6841 gataggtacg ttaattacaa aataaattta gaggtgtaaa ataatgagtg acattattaa
     6901 tgaaactaaa tcaagaatgc aaaaatcaat cgaaagctta tcacgtgaat tagctaacat
     6961 cagtgcagga agagctaatt caaatttatt aaacggcgta acagttgatt actatggtgc
     7021 accaacacct gtacaacaat tagcaagcat caatgttcca gaagcacgtt tacttgttat
     7081 ttctccatac gacaaaactt ctgtagctga catcgaaaaa gcgataatag cagctaactt
     7141 aggtgtcaac ccaacaagtg atggtgaagt gatacgtatt gctgtacctg ccttaacaga
     7201 agaacgtaga aaagagcgcg ttaaagatgt taagaaaatt ggcgaagaag ctaaagtatc
     7261 tgttcgaaat attcgtcgtg atatgaatga tcagttgaaa aaagatgaaa aaaatggcga
     7321 cattactgaa gatgagttga gaagtggtac tgaagatgtt cagaaagcaa cagacaattc
     7381 aataaaagaa attgatcaaa tgattgctga taaagaaaaa gatattatgt cagtataaaa
     7441 ctaatataca atgacatatt aaaatgccag tattaaacga taatgtaaca tttaaaatgg
     7501 gcatgtttaa ttaaatcaaa aatgcatgtg ataatttaaa ttcacaatga gcataaaaat
     7561 ggtgtttaaa caagttaatt aaacatatac tttataaata ataggcatca ggtatattgc
     7621 tataataaag ttatgtaatt ttcaacctca gtatgtatgt cacatttctg gtgtaaactg
     7681 taccgagtca gactttggta cagttttttt atttgcttat tcaatgcatt aaatgagtat
     7741 gataaaatga taatgattgt ttagtaactt atactatatg acagagatga tcaggctcgg
     7801 aggaaagacc atgtttaaaa agctaataaa taaaaagaac actataaata attataatga
     7861 agaattagac tcgtctaata tacctgaaca tatcgctatt attatggatg gtaatgggcg
     7921 atgggctaag aagcgaaaaa tgcctagaat taaaggtcat tacgaaggta tgcaaacaat
     7981 aaaaaaaatt actagggtag ctagtgatat tggtgttaag tacttaactt tatatgcctt
     8041 ttccactgaa aattggtcaa gacctgaaag tgaagtaaat tatattatga atttgcctgt
     8101 caatttctta aagacatttt taccggaact aattgaaaaa aatgtcaaag ttgaaacaat
     8161 tggatttact gataagttgc caaaatcaac gatagaagca ataaataatg ctaaagaaaa
     8221 gacagctaat aataccggct taaaattaat atttgcaatt aattatggtg gcagagcaga
     8281 acttgttcat agtattaaaa atatgtttga cgagcttcat caacaaggtt tgaatagtga
     8341 tatcatagat gaaacatata taaacaatca tttaatgaca aaagactatc ctgatccaga
     8401 gttgttaatt cgtacttcag gagaacaaag aataagtaat ttcttgattt ggcaagtttc
     8461 gtatagtgaa tttatcttta atcaaaaatt atggcctgac tttgacgaag atgaattaat
     8521 taaatgtatt aaaatttatc agtcacgtca aagacgcttt ggcggattga gtgaggagta
     8581 gtatagtatg aaagttagaa cgctgacagc tattattgcc ttaatcgtat tcttgcctat
     8641 cttgttaaaa ggcggccttg tgttaatgat atttgctaat atattagcat tgattgcatt
     8701 aaaagaattg ttgaatatga atatgattaa atttgtttca gttcctggtt taattagtgc
     8761 agttggtctt atcatcatta tgttgccaca acatgcaggg ccatgggtac aagtaattca
     8821 attaaaaagt ttaattgcaa tgagctttat tgtattaagt tatactgtct tatctaaaaa
     8881 cagatttagt tttatggatg ctgcattttg cttaatgtct gtggcttatg taggcattgg
     8941 ttttatgttc ttttatgaaa cgagatcaga aggattacat tacatattat atgccttttt
     9001 aattgtttgg cttacagata caggggctta cttatttggt aaaatgatgg gtaaacataa
     9061 gctttggcca gtaataagcc cgaataaaac aatcgaagga ttcataggtg gcttgttctg
     9121 tagtttgata gtgcctcttg caatgttata ttttgtagat ttcaatatga atgtatggat
     9181 attacttgga gtgacattga ttttaagttt atttggccaa ttaggtgatt tagtggaatc
     9241 aggatttaag cgtcatttcg gcgttaaaga ctcaggtcga atactacctg gacacggtgg
     9301 tattttagac cgatttgaca gctttatgtt tgtgttaccg ttattaaata ttttattaat
     9361 acaatcttaa tgctgagaac aaatcaataa acgtaaagag gagttgctga gataatttaa
     9421 tgaatctcag aactcctttt gaaaattata cgcaatatta acattgaaaa ttagacgtta
     9481 tattttgtga tttgtcagta tcatattata atgacttgtg ttacgtatac agcaatcatt
     9541 tttaaaataa aagaaattta taaacaatcg aggtgtagcg agtgagctat ttagttacaa
     9601 taattgcatt tattattgtt tttggtgtac tagtaactgt tcatgaatat ggccatatgt
     9661 tttttgcgaa aagagcaggc attatgtgtc cagaatttgc gatcggtatg gggccaaaaa
     9721 tttttagttt tagaaaaaat gaaacacttt acactattag gttattgcct gttggtggat
     9781 atgttcgtat ggcaggagat ggcttagaag agccaccagt cgagcctggt atgaacgtta
     9841 aaattaaact gaatgaagaa aatgaaataa cacatatcat attagatgat catcataagt
     9901 ttcaacaaat tgaagcgatc gaagtaaaaa aatgtgattt taaagatgac ttattcatag
     9961 aaggtatcac tgcttatgat aacgaaagac atcattttaa aattgctaga aagtctttct
    10021 ttgttgaaaa tggtagctta gttcaaattg ctccgagaga cagacaattt gcacataaaa
    10081 aaccatggcc gaaattttta acattatttg cgggaccgtt atttaacttt atattagctt
    10141 tagtcctatt tattggtctt gcatattatc aaggtacacc tacgtctact gtagaacaag
    10201 tcgcagataa gtatccagct caacaagcag gcttacaaaa aggcgataaa attgtccaaa
    10261 ttggtaaata taaaatatct gaatttgatg atgttgataa ggcgttagat aaagttaaag
    10321 ataataagac aactgttaaa tttgaacgtg atggtaaaac aaagtcagtt gaattaacac
    10381 ctaaaaagac tgaaagaaaa ctgactaaag taagttcaga aacgaagtat gttctcggat
    10441 tccaaccagc gagtgaacgt acgctcttca aaccaattgt atatggattt gaaagctttt
    10501 tgaaaggtag tacactaatt tttacagctg tagtaggtat gttggctagt atatttacgg
    10561 gcggattctc atttgatatg ttaaatggtc cggttggtat ttatcataac gtagactcag
    10621 ttgttaaagc gggtatcatt agcttaattg gatacactgc gttattaagt gtaaatttag
    10681 gtattatgaa tttaattcct attcctgcac tagacggtgg tcgtatttta tttgttatat
    10741 atgaagcgat tttcagaaaa ccagttaata aaaaagcgga aacaacgatt attgctattg
    10801 gtgccatttt catggtcgtt attatgatat tagtaacgtg gaatgatatt cgacgatatt
    10861 tcttataatt taggaggata aataattatg aagcaatcca aagtttttat accaacgatg
    10921 cgtgacgtgc catcagaagc agaagcacaa agtcatcgtt tattattgaa atcgggtttg
    10981 ataaaacaaa gtacaagtgg gatttatagt tatttaccgc tagcaacacg tgtgttaaat
    11041 aatattactg caattgtgcg acaagaaatg gaacgtatcg attctgttga aattttaatg
    11101 ccagcgttac aacaagctga attatgggaa gaatcaggac gttggggtgc atatggtcca
    11161 gaattaatgc gtttacaaga tagacatgga agacaatttg cattaggtcc aacacatgaa
    11221 gaattagtta catcaatagt aagaaatgaa ttgaaatcat acaaacaatt accgatgaca
    11281 ttattccaaa ttcaatctaa attccgtgat gaaaagagac cacgttttgg tttacttcgt
    11341 ggtcgtgaat ttattatgaa agatgcgtat tcattccatg ctgacgaggc atcattagat
    11401 caaacgtatc aagatatgta tcaagcgtat agccgtattt ttgagagagt tggcattaac
    11461 gcaagaccag tagttgcaga ttcaggtgct ataggcggta gccatacaca tgaatttatg
    11521 gcattaagtg ctatcggtga ggatacaatc gtttacagta aagaaagtga ctatgctgct
    11581 aatatcgaaa aagcagaagt cgtttacgaa ccaaatcata agcatactac tgtgcaacct
    11641 ttagaaaaaa ttgaaacacc aaatgttaag actgcgcaag aattggcaga cttcttaggt
    11701 agaccagtag atgaaatcgt taaaacgatg attttcaaag ttgatggcga atatattatg
    11761 gttttagtgc gtggacatca tgaaattaat gacattaaat taaaatctta tttcggcaca
    11821 gataatattg aattagcaac acaagacgaa attgttaatt tagttggtgc aaatcctggt
    11881 tcactaggtc ctgtaattga taaagaaatc aaaatttatg cagataattt tgtgcaagat
    11941 ttaaataatt tagttgtcgg tgctaacgaa gatggttatc acttaattaa tgtaaatgta
    12001 ggtagagact tcaacgttga tgaatatggc gatttccgtt ttattttaga aggcgaaaag
    12061 ttaagtgatg gttcaggcgt tgcacatttt gctgaaggta ttgaagttgg tcaagtattc
    12121 aaattgggta ctaagtattc agaatcaatg aatgctacat tcttagataa ccaaggaaaa
    12181 gcccaaccgt taattatggg ttgttacgga attggaattt ctagaacgct aagtgcgatt
    12241 gttgaacaaa atcacgatga taatggaatt gtttggccta aatcagtatc accatttgat
    12301 gtacatttaa tttcaattaa tcctaagaaa gatgatcaac gtgaacttgc agatcaactt
    12361 tatgcgaaat ttaatgagaa atttgatgta ttgtatgatg accgtcaaga acgtgctggt
    12421 gttaaattca atgatgcaga tttaataggc ttgccattgc gaatcgtagt tggtaaacgt
    12481 gcatcagaag gaattgttga agttaaagaa cgtttaacag gtgatagtga agaagtccac
    12541 attgatgact taatgactgt cattacaaat aaatatgata acttaaaata attaatatcg
    12601 aatgaattat aagggtagga aaaagctgaa agaaatctga tgcttatgtc ctgctcttat
    12661 tatttttgat ataatgatta ttcgatgaaa aatgactgaa gacatagtat aattaaagat
    12721 aaatttgttt taacaatatt atgattagcc aaatataaag catttaattt tctatcatta
    12781 ctatgcatac ataatctaaa tattgttcga acacgtaaaa gtaatttcta tttaaggtgg
    12841 taattgtctt ggcaatgaca gagcaacaaa aatttaaagt gcttgctgat caaattaaaa
    12901 tttcaaatca attagatgct gaaattttaa attcaggtga actgacacgt atagatgttt
    12961 ctaacaaaaa cagaacatgg gaatttcata ttacattacc acaattctta gctcatgaag
    13021 attatttatt atttatacat gcaatagagc aagagtttaa agatatcgcc aacgttacat
    13081 gtcgttttac ggtaacaaat ggcacgaatc aagatgaaca tgcaattaaa tactttgggc
    13141 actgtattga ccaaacagct ttatctccaa aagttaaagg tcaattgaaa cagaaaaagc
    13201 ttattatgtc tggaaaagta ttaaaagtaa tggtatcaaa tgacattgaa cgtaatcatt
    13261 ttgacaaggc ttgcaatgga agtcttatca aagcgtttag aaattgtggt tttgatatcg
    13321 ataaaatcat attcgaaaca aatgataatg atcaagaaca aaacttagct tctttagaag
    13381 cacatattca agaagaagat gaacaaagtg cacgtctagc tacagagaaa cttgaaaaaa
    13441 tgaaagctga aaaagcaaaa caacaagata ataacgaaag tgctgtcgat aagtgtcaaa
    13501 ttggtaagcc gattcaaatt gaaaatatta aaccaattga atctattatt gaggaagagt
    13561 ttaaagttgc aatagagggt gtcatttttg atattaactt aaaagaactt aaaagtggtc
    13621 gtcatatcgt agaaattaaa gtgactgact atacggactc tttagtttta aaaatgttta
    13681 ctcgtaaaaa caaagatgat ttagaacatt ttaaagcgct aagtgttggt aaatgggtta
    13741 gggctcaagg tcgtattgaa gaagatacat ttattagaga tttagttatg atgatgtctg
    13801 atattgaaga gattaaaaaa gcgacaaaaa aagataaggc tgaagaaaag cgtgtagaat
    13861 tccacttgca tactgcaatg agtcaaatgg atggtatacc aaatattggt gcgtatgtta
    13921 aacaggcggc agactgggga catccagcca ttgcggttac agaccataat gttgtgcaag
    13981 catttccaga tgctcacgca gcagcggaaa aacatggtat taaaatgata tacggtatgg
    14041 aaggtatgtt agttgatgat ggtgttccga ttgcatacaa accacaagat gtcgtattaa
    14101 aagatgctac ttatgttgta ttcgacgttg agacaactgg tttatcaaat cagtatgata
    14161 aaatcatcga gcttgcagct gtgaaagttc ataacggcga aatcatcgat aagtttgaaa
    14221 ggtttagtaa tccgcatgaa cgattatcgg aaacgattat caatttgacg catattactg
    14281 atgatatgtt agtagatgcc cctgagattg aagaagtact tacagaattt aaagaatggg
    14341 ttggcgatgc gatattcgta gcacataatg cttcgtttga tatgggcttc atcgacacgg
    14401 ggtacgaacg tcttggattt ggaccatcta cgaatggtgt tatcgataca ttagaattat
    14461 ctcgtacgat taatactgaa tatggtaaac atggtttaaa tttcttagct aaaaaatacg
    14521 gtgtagaatt aacgcaacat caccgtgcca tttatgatac agaggcaaca gcttacattt
    14581 tcataaaaat ggttcaacaa atgaaagaat taggcgtatt aaatcataac gaaatcaaca
    14641 aaaaactcag taatgaagat gcatataaac gtgcaagacc tagtcatgta acattaattg
    14701 tacaaaacca acaaggtctt aaaaatctat ttaaaattgt aagtgcatca ttggtgaaat
    14761 atttctaccg tacgcctcga attccacgtt cattgttaga tgaatatcgt gagggattat
    14821 tggtaggtac agcatgtgat gaaggtgaat tatttacggc agttatgcag aaggaccaga
    14881 gccaagttga aaaaattgcc aaatattatg attttattga aattcaacca ccggcacttt
    14941 atcaagattt aattgataga gagcttatta gagatactga aacattacat gaaatttatc
    15001 aacgtttaat acatgcaggt gacacagcgg gtattcctgt tattgcgaca ggaaatgcac
    15061 actatttgtt tgaacatgat ggtattgcac gtaaaatttt aatagcatca caacccggca
    15121 atccacttaa tcgctcaact ttaccggaag cacattttag aactacagat gaaatgttaa
    15181 acgagtttca ttttttaggt gaagaaaaag cgcatgaaat tgttgtgaaa aatacaaacg
    15241 aattagcaga tcgaattgag cgtgttgttc ctattaaaga tgaattatac acaccgcgta
    15301 tggaaggtgc taacgaagaa attagagaac taagttatgc aaatgcgcgt aaactgtatg
    15361 gtgaagacct gcctcaaatc gtaattgatc gattagaaaa agaattaaaa agtattatcg
    15421 gtaatggatt tgcggtaatt tacttaattt cgcaacgttt agttaaaaaa tcattagatg
    15481 atggatactt agttggttcc cgtggttcag taggttctag ttttgtagcg acaatgactg
    15541 agattactga agtaaacccg ttaccgccac actatatttg tccgaactgt aaaacgagtg
    15601 aatttttcaa tgatggttca gtaggatcag gattcgattt acctgataag acgtgtgaaa
    15661 cttgtggagc gccacttatt aaagaaggac aagatattcc gtttgaaaca tttttaggat
    15721 ttaaaggaga taaagttcct gatatcgact taaactttag tggtgaatat caaccgaatg
    15781 cccataacta cacaaaagta ttatttggtg aggataaagt attccgtgca ggtacaattg
    15841 gtactgttgc tgaaaagact gcttttggtt atgttaaagg ctatttgaat gatcaaggca
    15901 tccacaaaag aggcgctgaa atagatcgac tcgttaaagg atgtacaggt gttaaacgta
    15961 caactggaca gcatccaggg ggtattattg tagtacctga ttacatggat atttatgatt
    16021 ttacgccgat acaatatcct gccgatgatc aaaattcagc gtggatgacg acacattttg
    16081 atttccattc tattcatgat aatgtattaa aacttgatat acttggacac gatgatccaa
    16141 caatgattcg tatgcttcaa gatttatcag gaattgatcc aaaaacaata cctgtagatg
    16201 ataaagaagt tatgcagatt tttagtacac ctgaaagttt gggtgttact gaagatgaaa
    16261 ttttatgtaa aacaggtaca tttggggtac cagaattcgg tacaggattc gtgcgtcaaa
    16321 tgttagaaga tacaaagcca acaacatttt ctgaattagt tcaaatctca ggattatctc
    16381 atgggacaga tgtgtggtta ggtaatgctc aagaattaat taaaacaggt atatgtgatt
    16441 tatcaagtgt aattggttgt cgtgacgata tcatggttta tttaatgtat gctggtctag
    16501 aaccatcaat ggcttttaaa ataatggagt cagtacgtaa aggtaaaggt ttaactgaag
    16561 aaatgattga aacgatgaaa gaaaatgaag tgccggattg gtatttagat tcatgtctta
    16621 aaattaagta catgttccct aaagcccatg cagcagcata tgttttaatg gcagtacgta
    16681 tcgcatattt caaagtacat catccacttt attactatgc atcttacttt acaattcgtg
    16741 cgtcagattt tgatttaatc acgatgatta aagataaaac aagcatacga aatactgtaa
    16801 aagacatgta ttctcgctat atggatctag gtaaaaaaga aaaagacgta ttaactgtct
    16861 tggaaattat gaatgaaatg gcgcatcgag gttatcgaat gcaaccgatt agtttagaaa
    16921 agagtcaagc gttcgaattt atcattgaag gcgatacact aattccgccg ttcatatcag
    16981 tgcctgggct tggcgaaaac gttgcgaaac gaattgttga agctcgtgac gatggaccat
    17041 ttttatcaaa agaagattta aacaaaaaag ctggtttatc tcagaaaatt attgagtatt
    17101 tagatgagtt aggctcatta ccgaatttac cagataaagc tcaactttcg atattcgata
    17161 tgtaaaatga aataatcaag gtatttattt aatgcgtatg gcgtagttaa agaaatacaa
    17221 aattattgtt ggacacaaag ttatgcctgt atttcttttc aatgtcttac gagtctattc
    17281 gaatgtaatg gtgaaataaa tgaacaaact tttgcaagaa tctctgatta atagtgaagt
    17341 catttgtttc aagcataaac ttatgctata attaagttgc ttaaaaatta gtgaactcag
    17401 gcagaagagt gggagattcc cgctcttttc tatttgccaa aaagggaggc ctgtatgagt
    17461 aaaattacag aacaagtaga agtgattgtt aaaccaatta tggaagactt gaattttgaa
    17521 cttgtagacg ttgaatatgt caaagagggt agagatcatt ttcttagaat ctctattgat
    17581 aaagaaggtg gcgtagattt aaatgattgt acgctagctt ctgaaaaaat aagtgaagct
    17641 atggatgcaa atgatcctat tcctgaaatg tattatttag acgtagcgtc acctggtgca
    17701 gaacgtccaa ttaaaaaaga acaagatttc caaaatgcaa taactaaacc tgtatttgtt
    17761 tctttatatg taccaattga aggtgaaaag gaatggttag gcattttaca agaagtcaat
    17821 aatgaaacaa ttgtagtaca agttaaaatc aaagcaagaa cgaaagatat agagataccg
    17881 agagacaaaa tagcaaaagc acgtcacgca gttatgattt aacgtgatga ggaggaaaaa
    17941 acgtgtcaag taatgaatta ttattagcta ctgagtattt agaaaaagaa aagaagattc
    18001 ctagagcagt attaattgat gctattgaag cagctttaat tactgcatac aaaaagaatt
    18061 atgatagtgc aagaaatgtc cgtgtggaat taaatatgga tcaaggcact ttcaaagtta
    18121 tcgctcgtaa agatgttgtt gaagaagtat ttgacgacag agatgaagtg gatttaagta
    18181 cagcgcttgt taaaaaccct gcatatgaaa ttggtgatat atatgaagaa gatgtaacac
    18241 ctaaagattt tggtcgtgta ggtgctcaag cagcgaaaca agcagtaatg caacgtcttc
    18301 gtgatgctga acgtgaaatt ttatttgaag aatttataga caaagaagaa gacatactta
    18361 ctggaattat tgaccgtgtt gaccatcgtt atgtatatgt gaatttaggt cgtatcgaag
    18421 ctgttttatc tgaagcagaa agaagtccta acgaaaaata tattcctaac gaacgtatca
    18481 aagtatatgt taacaaagtg gaacaaacga caaaaggtcc tcaaatctat gtttctcgta
    18541 gccatccagg tttattaaaa cgtttatttg aacaagaagt tccagaaatt tacgatggta
    18601 ctgtaattgt taaatcagta gcacgtgaag ctggcgatcg ctctaaaatt agtgtcttct
    18661 ctgaaaacaa tgatatagat gctgttggtg catgtgttgg tgccaaaggc gcgcgtgtag
    18721 aagctgttgt tgaagagcta ggtggcgaaa aaatcgacat cgttcaatgg aatgaagatc
    18781 caaaagtatt tgtaaaaaat gctttaagcc cttctcaagt tttagaagtt attgttgatg
    18841 aaacaaatca atctacggta gttgttgttc ctgattatca attgtcatta gcgattggta
    18901 aaagaggaca aaacgcacgt ctagctgcta aattaaccgg ctggaaaatt gatattaaat
    18961 cagaaacaga tgcgcgtgaa gcgggtatct atccagtagt tgaagctgaa aaagtaactg
    19021 aagaagatgt tgctttagaa gatgctgaca caacagaatc aaccgaagag gtaactgatg
    19081 tttcagttga aacaaatgta gagaaagaat ctgaataata ggttggagtg aagtatctat
    19141 gaaaaagaaa aaaattccga tgcgaaaatg tattctttca aatgaaatgc atcccaaaaa
    19201 agatatgatt cgtgttgttg ttaataaaga aggcgaaatc tttgcggatg ttactggaaa
    19261 gaaacaaggc cgtggcgcat atgtttctaa agatgttgct atggttgaaa aagcacaaca
    19321 aaaagaaatt ttagaaaaat attttaaagc atctaaagag caattggatc ctgtttacaa
    19381 agaaattatt agattaattt acagagaaga gatcccaaaa tgagtataga tcaaatatta
    19441 aactttttag gattagcaat gagagctggt aaagtaaaaa caggtgaatc agtcattgtt
    19501 aatgagatta aaaaaggaaa tttgaagctc gttattgttg caaatgatgc gtctgataat
    19561 acagctaaat taattacaga taaatgtaag agttacaaag ttccattcag aaagtttgga
    19621 aatcgaaatg aattgggaat agcacttgga aaaggtgagc gtgttaatgt agggattact
    19681 gacccaggct ttgctaaaaa gttgctatca atgatagatg aatatcataa ggagtgatta
    19741 tatgagtaaa caaagaattt acgaatatgc gaaagaatta aatctaaaga gtaaagagat
    19801 tatagatgag ttaaaaagca tgaatattga ggtttcaaat catatgcaag ctttggaaga
    19861 tgaccaaatt aaagcattag ataaaaagtt caaaaaagaa caaaagaacg acaataaaca
    19921 aagcactcaa aataatcacc aaaaatcaag caatcaaaac caaaataaag ggcaacaaaa
    19981 agataacaaa aagaatcaac aacaaaataa taaaggcaat aaaaagaata atagaaataa
    20041 taagaaaaat aacaagaata ataaaccaca aaatcaacca gccgctccaa aagaaatacc
    20101 atcaaaagtg acatatcaag aaggtattac agtaggcgaa tttgcggata aattaaatgt
    20161 tgaatcatca gaaattatca aaaaattatt cttacttggt attgttgcta atatcaatca
    20221 atcattaaat caagaaacaa tcgaattaat tgccgatgat tatggcgttg aggttgaaga
    20281 agaagttgtg attaatgaag aagatttatc aatctacttc gaagacgaaa aagatgatcc
    20341 agaagcaatt gagagaccag cagttgtaac aattatggga catgttgacc atggtaaaac
    20401 tactttatta gattcaattc gtcatacaaa agttacagca ggtgaagcag gcggaatcac
    20461 tcaacatatt ggtgcatatc aaattgaaaa cgatggcaaa aaaatcactt tcttagatac
    20521 accgggacat gctgcattta caacgatgcg tgcgcgtggt gcacaagtaa cagatattac
    20581 aattttagta gtagcagctg acgatggtgt tatgccacaa acaattgaag caattaacca
    20641 tgctaaagaa gcagaagtac caattattgt tgcagtaaat aaaattgata aaccaacttc
    20701 aaatcctgat cgagttatgc aagaattaac tgaatatggt ttaattcctg aagattgggg
    20761 cggcgaaaca attttcgttc cgctttctgc attaagtggt gatggtatcg acgatttatt
    20821 agaaatgata ggattagttg cagaagttca agaacttaaa gcaaatccta aaaaccgtgc
    20881 tgttggtaca gttatcgaag ctgaattaga taaatcacgt ggtccttctg catcattatt
    20941 agtacaaaac ggtacattaa atgttgggga tgcgattgta gttggtaata cttacggccg
    21001 tatccgtgca atggttaatg acttaggtca aagaatcaaa acggctggcc catcaacgcc
    21061 tgttgaaatt acaggtatta atgatgtgcc acaagctggg gatcgctttg ttgtatttag
    21121 tgatgaaaaa caagctcgtc gtattggtga atcaagacac gaagctagca ttgtacaaca
    21181 acgtcaagaa agtaaaaatg tttcattaga taacctgttt gaacaaatga aacaaggtga
    21241 aatgaaagac ttaaacgtta ttattaaagg tgatgttcaa ggttctgttg aagctttagc
    21301 tgcatcatta atgaaaattg atgttgaagg cgtaaatgtt cgtatcattc atacagcggt
    21361 tggtgcgatt aatgagtcag acgtgacact tgctaatgcc tcaaatggta ttatcattgg
    21421 tttcaatgtt cgtccagaca gtggtgcaaa acgtgctgca gaagctgaaa atgttgatat
    21481 gcgtttacac agagttattt ataatgttat cgaagaaatt gaatcagcga tgaaaggttt
    21541 acttgatcca gaatttgaag aacaagttat cggacaagct gaagttcgtc aaacattcaa
    21601 agtttctaaa gttggtacta ttgctggatg ttatgttact gaaggtaaaa ttacgcgaaa
    21661 tgctggtgta cgtattattc gtgatggtat tgttcaatat gaaggcgaat tagatacact
    21721 taaacgtttc aaagatgatg ctaaggaagt tgcaaaaggt tatgaatgtg gtattacaat
    21781 tgaaaactac aatgacctta aagaaggcga tgttatcgaa gcatttgaaa tggttgaaat
    21841 taagcgttaa ttaaataaat tacaagctaa aagtatagtt aagattgata tgctccctat
    21901 aaatattgca ctttttaagt gtctacttta tagggagcat atttgatgct agcttttggt
    21961 tttttattag aatagtttac ctattaaaag ttacgttata tagacatgat tttgtataaa
    22021 attttgtggt ggcctagaat gatttttaat gacaaaatat aatgtcgact attattggaa
    22081 aattttctgt tgaaatgcct atcttacggc aaactttatt tgattttata ggcttaattt
    22141 attaaaataa cgtgtgagct aaaataattg tttaagcatt gttacactaa aaaatgcaaa
    22201 taacaattga acttaaagat aaagaggtga caagaatgag cagtatgaga gcagaacgtg
    22261 ttggtgaaca aatgaagaag gaattaatgg atatcatcaa caataaagtc aaagatcctc
    22321 gagttggttt tattacaatt actgatgttg ttttaacaaa tgatttatcg caggctaaag
    22381 tatttttaac tgtattaggt aacgataaag aagtagaaaa tacatttaaa gcacttgata
    22441 aagcaaaagg cttcattaag tctgaattag gttctagaat gcgattacgt attatgccgg
    22501 aattaatgta tgaatatgat caatcaatcg aatatggtaa taaaattgaa cgaatgattc
    22561 aagatttaca caaacaagat agataattta gtgttaggta tctggaaaat gtttgataat
    22621 ttcttaatat cggtatatta acattaaaca gttaatacat agatgtgtag aaatagttaa
    22681 cattttccag tttttttatg aataaattca gttaatacgc tattaaaata tatttttaaa
    22741 aaagaaggtg acaacatgta taacggtata ttaccagtat ataaagaacg tggtttaaca
    22801 agtcatgacg tcgtattcaa attgcgtaaa attttaaaga ctaaaaaaat agggcacact
    22861 ggtacgcttg atcctgaagt tgcaggcgtg ttaccggtat gtataggtaa tgcaacgaga
    22921 gttagtgatt atgttatgga tatgggcaaa gcttatgaag caactgtatc gataggaaga
    22981 agcacaacaa ctgaagatca aacgggtgat acattggaaa tgaagggtgt acattcagct
    23041 gatttcaaca atgacgatat tgaccgcttg ttagaaaatt ttaaaggtgt tattgaacaa
    23101 attccgccaa tgtattcatc agtcaaggtg aatggtaaaa aattatatga atatgcgcgt
    23161 aataatgaaa cagttgaaag accaaagcgt aaagttaata ttaaagacat tgggcgtata
    23221 tctgaattag attttaaaga aaatgagtgt cattttaaaa tacgcgtcat ctgtggtaaa
    23281 ggtacatata ttagaacgct agcaactgat attggtgtga aattaggttt tccggcacat
    23341 atgtcgaaat taacgcgaat cgagtctggt ggatttgtgt tgaaagatag ccttacatta
    23401 gaacaaataa aagaacttca tgagcaggat tcattgcaaa ataaattgtt tcctttagaa
    23461 tatggattaa agggtttgcc aagcattaaa attaaagatt cgcacataaa aaaacgtatt
    23521 ttaaatgggc agaaatttaa taaaaatgaa tttgataaca aaattaaaga ccaaattgta
    23581 tttattgatg atgattcaga aaaagtatta gcaatttata tggtacaccc tacaaaagaa
    23641 tcagaaatta aacctaaaaa agtctttaat taaaggagat agaatttatg aaagtcatag
    23701 aagtgacaca tcctatacaa tctaaacagt atattacaga ggatgttgca atggcattcg
    23761 gatttttcga tggcatgcat aaaggtcatg acaaagtctt tgatatatta aacgaaatag
    23821 ctgaggcacg cagtttaaaa aaggcggtga tgacatttga tccgcatccg tctgtcgtgt
    23881 tgaatcctaa aagaaaacga acaacgtatt taacaccact ttcagataaa atcgaaaaaa
    23941 ttagccaaca tgatattgat tattgtatag tggttaattt ttcatctagg tttgctaatg
    24001 tgagcgttga agattttgtt gaaaattata taattaaaaa taatgtaaaa gaagtcattg
    24061 ctggttttga ttttactttt ggtaaatttg gaaaaggtaa tatgactgtt cttcaagaat
    24121 atgatgcgtt taatacgaca attgtgagta aacaagaaat tgaaaatgaa aaaatttcta
    24181 caacttctat tcgtcaagat ttaatcaatg gtgagttgca aaaagcgaat gatgctttag
    24241 gctatattta ttctattaaa ggcactgtcg tgcaaggtga aaaaagggga agaactattg
    24301 gcttcccaac agctaacatt caacctagtg atgattattt gttacctcgt aaaggtgttt
    24361 atgccgttag tattgaaatc ggcactgaaa ataaattata tcgaggggta gctaacatag
    24421 gtgtaaagcc aacatttcat gatcctaaca aagcagaagt tgtcatcgaa gtgaatatct
    24481 ttgactttga ggataatatt tatggtgaac gagtgaccgt gaattggcat catttcttac
    24541 gtcctgagat taaatttgat ggtatcgacc cattagttaa acaaatgaac gatgataaat
    24601 cgcgtgctaa atatttatta gcagttgatt ttggtgatga agtagcttat aatatttaga
    24661 gttgcgtata gttatataga caatctatac cacacctttt tcttagtagg tcgaatctcc
    24721 aacgcctaac tcggattaag gagtattcaa acattttaag gaggaaattg attatggcaa
    24781 tttcacaaga acgtaaaaac gaaatcatta aagaataccg tgtacacgaa actgatactg
    24841 gttcaccaga agtacaaatc gctgtactaa ctgcagaaat caacgcagta aacgaacact
    24901 tacgtacaca caaaaaagac caccattcac gtcgtggatt attaaaaatg gtaggtcgtc
    24961 gtagacattt attaaactac ttacgtagta aagatattca acgttaccgt gaattaatta
    25021 aatcacttgg tatccgtcgt taatcttaat ataacgtctt tgaggttggg gcatatttat
    25081 gttccaacct taatttatat taaaaaagct ttttacaaat attaacattt attatatgtt
    25141 aagctaatat tgagtgaata ataaggttac aatgagataa agatgatata agtacaccta
    25201 gagtaataat caagatatta aaaataaagt atgtttttta aaaaaatata acttatattt
    25261 atactgataa gggtgggacg ataagtctat tttgtaaata atagatggat atcccgctct
    25321 ctttttttcc aattcaatat tttataacta atattaaaat acgataataa atgatatgat
    25381 ataactatta gattcaagag aggagattta taatgtctca agaaaagaaa gtttttaaaa
    25441 ctgaatgggc aggaagatct ttaacgattg aaacagggca attagctaaa caagcaaatg
    25501 gcgctgtatt ggttcgttat ggagatacag tcgtgttatc gacggcaact gcatcaaaag
    25561 aacctcgtga tggagatttc ttcccattaa cagtgaacta tgaagaaaaa atgtacgctg
    25621 cgggtaaaat tcctggtgga tttaaaaaga gagaaggacg tcctggtgac gatgcaacat
    25681 taactgcgcg attaattgat agaccaatta gacctttatt ccctaaagga tataagcatg
    25741 atgttcaaat tatgaacatg gtattaagtg cagatcctga ttgttcacca caaatggctg
    25801 caatgattgg ttcatctatg gcgcttagtg tgtcggatat tccattccaa gggccaatcg
    25861 ccggtgtaaa tgtgggttat attgacggta aatatatcat taacccaaca gtagaagaaa
    25921 aagaagtttc tcgtttagac cttgaagtag ctggtcataa agatgcagta aacatggtag
    25981 aggcaggcgc tagtgagatt actgaacaag aaatgttaga ggcgattttc tttggtcatg
    26041 aagagattca acgtttagtt gatttccaac aacaaatcgt cgaccacatt caacctgtta
    26101 aacaagaatt tattccagca gagcgtgatg aagcgctagt tgaacgtgta aaatctttaa
    26161 ccgaagaaaa aggacttaaa gaaacagttt taacatttga taaacaacaa cgagatgaaa
    26221 atcttgataa cttaaaagaa gaaatcgtca atgaatttat cgatgaagaa gatccagaga
    26281 atgaattact tattaaagaa gtttatgcaa ttttaaatga attagtgaaa gaagaagttc
    26341 gacgtttaat tgcagatgaa aaaattagac cagacggccg taaacctgat gaaatccgtc
    26401 cattagattc tgaagttggt attttaccta gaacgcatgg ttcaggtcta tttacacgtg
    26461 gtcagactca agcactttca gttttaacgt taggtgcttt aggcgattat caattaattg
    26521 atggtttagg acctgaagaa gaaaaaagat tcatgcatca ttataacttc ccgaattttt
    26581 cagtaggtga aactggtcca gtacgtgcgc caggtcgtcg tgaaattgga catggtgcgt
    26641 taggtgaaag agcattaaaa tatattattc ctgatactgc tgatttccca tatacaattc
    26701 gtattgtaag tgaggtactt gaatcaaacg gttcatcatc tcaagcgtca atttgtggat
    26761 caacattagc attaatggat gcgggagtac cgattaaagc accagttgct ggtattgcta
    26821 tgggccttgt tacacgtgaa gatagctata cgattttaac tgatatccaa ggtatggaag
    26881 atgcattagg tgatatggac tttaaagtcg ctggtactaa agaaggtatt acagcaatcc
    26941 aaatggatat taaaattgac ggtttaacgc gtgaaattat cgaagaggct ctagaacaag
    27001 cgagacgtgg tcgtttagaa ataatgaatc atatgttaca aacaattgat caaccacgta
    27061 ctgaattaag tgcttacgcg ccaaaagttg taactatgac aattaaacca gataagatta
    27121 gagatgttat cggacctggt ggtaaaaaaa tcaacgaaat tattgatgaa acaggcgtta
    27181 aattagatat tgaacaagat ggtactatct ttattggtgc cgttgatcaa gctatgataa
    27241 atcgtgctcg tgaaatcatt gaggaaatta cacgtgaagc ggaagtaggt caaacttatc
    27301 aagccactgt taaacgtatt gaaaaatacg gtgcgtttgt aggcctattc ccgggtaaag
    27361 atgcgttgct tcacatttca caaatttcaa aaaatagaat tgaaaaagtg gaagatgttt
    27421 taaaaatcgg tgacaaaatt gaagttaaga ttactgaaat tgataaacaa ggtcgagtaa
    27481 atgcttcaca cagagcatta gaagaataat atttaaagtc atatgacgac aatgtatcgt
    27541 catgtgattt ttttatgcca ttttttacga agtgatccat tttgaatttg ttgtattgaa
    27601 cattttaaaa cgctttatta ttttgtgtgc aactgttaat tatcctgtat gtatagtgat
    27661 taatagtgta catcaagtgt tttttaactt ataatgaata gtgagtttat atatggacgg
    27721 gtaacaaatt taggaggtaa gattttgagt ttaataaaga aaaagaataa agatattcgc
    27781 attataccat taggcggtgt tggcgaaatt gctaaaaata tgtatatcgt tgaagtagac
    27841 gatgaaatgt ttatgttaga tgctggactt atgtttccag aagacgaaat gctaggtatt
    27901 gatattgtta taccagacat ttcatacgta cttgaaaata aagataaatt gaaggggata
    27961 ttccttacac acggacatga gcacgcgatt ggtgcagtga gttatgtttt agaacaatta
    28021 gatgcaccag tatatggatc taaattgaca atagcgttaa ttaaagaaaa tatgaaagcc
    28081 cgtaatattg ataaaaaagt tcgctactac acagttaata atgattcaat tatgagattc
    28141 aaaaacgtga atattagttt ctttaatacg acacacagta ttcctgatag tttaggtgtt
    28201 tgtattcaca cttcatatgg tgccattgtg tatacaggtg aatttaagtt tgaccaaagt
    28261 ttacatggac attatgcacc agatattaaa cgtatggcag agattggtga agaaggtgta
    28321 tttgtcttaa tcagtgattc tactgaggca gagaaacctg gatataatac tccggaaaat
    28381 gtgattgaac atcatatgta tgatgctttt gcaaaagtgc gaggtcgctt gatagtttca
    28441 tgttatgctt cgaactttat acgtattcag caagttttaa atattgctag caagctaaat
    28501 cgtaaagtgt catttttagg aagatcactt gaaagttcat ttaatattgc tcgtaaaatg
    28561 ggatatttcg acattcctaa agatttgcta attcctataa cagaagttga taattatcct
    28621 aaaaatgaag tgataattat agctactggt atgcaaggag aacctgtaga agccttaagt
    28681 caaatggcgc aacataagca taaaattatg aatatcgaag aaggcgattc tgtattttta
    28741 gcaattacgg cttctgctaa tatggaagtt atcattgcga atacattaaa tgagcttgta
    28801 cgtgctggcg cacatattat tccaaataac aagaagattc atgcttcaag tcacggttgc
    28861 atggaagaat taaaaatgat gattaatatt atgaaacctg aatactttat tcctgtacaa
    28921 ggtgaattta aaatgcagat agcacatgcg aagctagcag ctgaagcagg tgttgcacca
    28981 gaaaagattt tccttgtgga aaaaggagat gtcattaatt acaacggtaa agatatgata
    29041 ttaaatgaaa aggtaaattc aggaaatatt ttaatagatg gtattggtat tggggatgta
    29101 ggaaatatcg tgttgagaga ccgtcatctt ttagcagaag atggtatctt tattgctgta
    29161 gtaacgttag atcctaaaaa tagacgtata gctgcgggac ctgaaattca atctcgtgga
    29221 tttgtatatg tacgtgaaag tgaagactta ttacgtgaag cagaagagaa agtacgtgaa
    29281 atagtagaag ctggtttaca agaaaaacgc atagaatggt ctgaaattaa gcaaaatatg
    29341 cgtgatcaaa ttagtaaact attattcgaa agtacaaaac gtcgtcctat gattattcca
    29401 gtaatttctg aaatttaatc aaaaagtcat taacataaaa gaggtcagaa caagtcaccg
    29461 aaatataatg gttgtcatgg acaatttact tatattttat gatggtcaat tgaaaggggt
    29521 aacgattaat ctgttatctt aagtaaattg atacatagat gatattgttc taacctcttt
    29581 catcgtctgt ttggactaca tattctaaac atcaaatagg aaattatata taataacgtc
    29641 gttttaacta aggtaacata aggaggtgcg tcaattggca caagcaaaaa agaaatcgac
    29701 agctaagaaa aaaacaacat caaaaaaaag aacaaattcg aggaaaaaga agaatgataa
    29761 tccgatacgt tatgtcatag ctattttagt agttgtatta atggtgttgg gtgttttcca
    29821 attaggaata ataggtcgtc taattgacag cttctttaat tatttatttg ggtacagtag
    29881 atatttaaca tatattttag tactcttagc aactggtttt attacatact ctaaacgtat
    29941 tcctaaaact agacgaacgg ctggttcgat tgtattgcaa attgcattgc tatttgtatc
    30001 acagttagtt tttcatttta atagtggtat caaagctgaa agagaacctg tactttctta
    30061 tgtgtatcag tcataccaac acagtcattt tccaaatttt ggtggcggtg tattaggctt
    30121 ttacttatta gagttaagcg tacctttaat ttcattattt ggtgtatgta ttattactat
    30181 tttattatta tgctcaagtg ttattttatt aacaaaccat caacatcgtg atgttgcaaa
    30241 agttgcactg gaaaatataa aagcttggtt tggttcattt aatgaaaaaa tgtcggaaag
    30301 aaaccaagaa aaacaattga agcgtgaaga aaaagcgaga cttaaagaag aacaaaaggc
    30361 acgtcaaaat gaacggccac aaataaaaga tgtgagtgat tttacggaag tgcctcaaga
    30421 aagagatatt ccaatttatg ggcatactga aaatgaaagt aaaagccaga gtcaaccaag
    30481 tcgaaaaaaa cgagtgtttg atgcagagaa tagttcgaat aacatcgtaa atcatcatca
    30541 agcagatcag aaagaacaat taacagaaca aactcataac agtgttgaaa gtgaaaacac
    30601 tattgaagaa gctggtgaag ttacgaatgt gtcgtatgtt gttccaccgt taactttact
    30661 taatcaacct gcaaaacaaa aagcaacatc taaagctgaa gtgcaacgta aaggacaagt
    30721 actagagaat acattaaaag attttggggt aaatgcaaaa gtgacacaaa ttaaaattgg
    30781 tcctgcagta actcaatatg aaattcaacc agctcaaggg gttaaagtga gtaaaattgt
    30841 aaacttgcat aatgatattg cattagcttt agcagcaaaa gatgttagaa tcgaagcgcc
    30901 aatacctggt cgctctgcag taggtattga agtgccaaat gagaaaattt cattagtttc
    30961 actaaaagaa gttttagatg aaaaattccc gtctaataat aaactagaag ttggattagg
    31021 aagagatata tcaggtgatc caattactgt tccactaaat gaaatgccac acttattggt
    31081 ggcaggatcg acgggtagtg gtaaatctgt ttgtataaat ggtattatta caagtatttt
    31141 attaaatgct aagccgcatg aagttaaact tatgttaatc gatccgaaaa tggttgaact
    31201 aaatgtttat aacggaattc cacatttatt aattccggtt gttacaaatc ctcataaagc
    31261 tgctcaagct ttagaaaaaa ttgtagctga gatggaaaga cgttatgatt tattccaaca
    31321 ttcatcaact agaaatatta aaggttataa tgaattaatc cgtaagcaaa atcaagaatt
    31381 agatgagaag caaccagaat taccttatat cgttgttatt gtagatgagc ttgcagattt
    31441 aatgatggta gctggtaaag aagttgaaaa tgcgattcaa cgtatcacac aaatggcacg
    31501 tgcagcaggt atacatttga ttgtagcgac acaaagacct tctgtggatg taattacagg
    31561 tatcattaaa aataatattc catctagaat tgcttttgct gtgagttctc aaacagattc
    31621 aagaactatt attggtactg gcggcgcaga aaagttactt ggtaaaggtg acatgttata
    31681 cgttggaaat ggtgactcat cacaaacacg tattcaaggg gcgtttttaa gtgaccaaga
    31741 ggtgcaagat gttgtaaatt atgtagtaga acaacaacag gcaaattatg taaaagaaat
    31801 ggaaccagat gcaccagtgg ataaatcgga aatgaaaagt gaagatgctt tatatgatga
    31861 agcgtatttg tttgttgttg aacaacaaaa ggcaagtaca tcattgttac aacgccaatt
    31921 tagaattggt tataatagag catctaggtt gatggatgat ttagaacgca atcaggtaat
    31981 cggtccacaa aaaggaagca agcctagaca agttttaata gatcttaata atgacgaggt
    32041 gtaaaaaaat gtcagaaatg aatgcggtat ataacgttaa acaatacatt ttaaatttga
    32101 ttaagcaaaa taaattggaa tatggtgacc aacttccaag taacttatca attgccagag
    32161 aattaaatgt aaaaaccgac gatgtttatg aagcaattca ggcattgatt actgaacaag
    32221 tcattaaaga taattttgaa gagggcacaa gtgttaagtc actgccccct ttcttttatc
    32281 cattgaatga acttataagt attgggcaaa tgattaaaaa tgcaggattt gaatgcggaa
    32341 ctgaatactt aaattttgat gagcaaccag caactatgtt agatgcaaat ttgttgagcg
    32401 ttgaagaagg atatccagta accattatag aacgattacg aactgccgat ggagaaccgg
    32461 tcgtctattg tttagataaa attgctaaaa aagaattaac atgtacagag tatcaaatga
    32521 gcaatggatc gatactaagt gcgataaaag aacaaagtaa tcataatatt tgttacgctg
    32581 atacagaaat tgaagcggta aattatgaac ctcgaatatc cgaagtactt aatgcttcgc
    32641 cacacgaagg tttgatttta ttaaaaatta cgcactacaa tgaattagat gaacctattt
    32701 tgtattcatt aaattatatg aaaaatagct tagttcaatt taaaatcact agaaaaatat
    32761 agatgtaaga agatatagag gaggctatat atttgagtag acaatctcaa ccaaatatac
    32821 atatcaaagt ttcaccaaca actaaattta aaacaactac tatagttttt aaatttatgg
    32881 cacctttaga atatgacaca ataacagcta gatcattatt aagcaaatta ttagttcgag
    32941 caactaagaa atggccaacc gataagtcat ttaataatca cttagccgat ttatatggtg
    33001 cgtatgtgaa tagtacaatt tcaaaattca aagatcagca tgtcattaca ttttcattag
    33061 aaattgttaa tgaacgttat ttaagaaacg gtgaatcatt atttaatcaa ggattagatt
    33121 tattacaaga aatcatttgg aatccattaa ttgaaaataa agcattcaat gataattttg
    33181 ttaaccaaga gaaaacatta ttagccaaaa aaatagaagc aatggtagat aacaaagcac
    33241 aatattcgtt tttaaaatta ctcgaccata tgtttgaaaa tgaagcatac aaatacttat
    33301 ctacaggaca actagaacaa atcccacata ttactgctga aacactatat catacatatc
    33361 aatcaatgat taataatgat caatgttctg tttatgttgt cggcaatgta gaacctgaaa
    33421 gtgttaagaa acaaatacgt gaaaaatttg cacttaaacc attcgataaa catcaattcc
    33481 aacattctac tcatcattta cacgatgaag aagttgatta tattgttgaa tatgatgacg
    33541 tggatcaagc taaattaaat atgggatacc gttttccaac acaatatgga caaagtggat
    33601 atgctgcctt tgttgtattt aatatgatgt ttggaggaga tccttcatct gttttattta
    33661 atgaagtgcg agaaaagcaa agtttagcgt actctataca ttcacaaatt gatggcaaaa
    33721 atggctattt atttgttttg agtggggttt caagtgataa gtacgaaact gcaaaagaca
    33781 ctattataag tgaatttgaa aaaataaaag caggagattt cactgaagaa aaattagagt
    33841 tagctaaaaa agtaatcatt tctcatcgat atgaatctga agatcgtccg aaaagtatta
    33901 tagagattat gcataatcaa atattattag agcaaccaca aagcaaagaa agatttatta
    33961 atgatataca gaaggtaagt cgcgaagata ttgtttctgt tgctgaaaaa gcatttttag
    34021 atacaatcta tgtgttgaca aaaggagggg ataaataatg aaagagcgtt attatgaatt
    34081 aatagacgaa agagtattcg aacaagaatt agaaaatggt ttacgattat ttattatccc
    34141 caaaccaggt tttcaaaaga catttgtcac ttacactaca caatttggtt cattagataa
    34201 tcaattcaaa ccccttggac aagaccaatt tgttactgta cctgatggag ttgctcactt
    34261 tttagaacat aaattatttg aaaaagaaga agaagactta tttactgcgt ttgctgaaga
    34321 taatgcacaa gcaaatgcgt ttacaagctt tgatcgtaca agctacttgt tcagtgcaac
    34381 tgataatatt gaaaacaaca ttaaacgttt acttacaatg gttgaaacgc cttattttac
    34441 aaaagaaact gttgataaag aaaaaggtat tattgcagaa gaaataaaaa tgtatcaaga
    34501 acaacctgga tataaattaa tgtttaatac attgcgtgca atgtatcaac aacatccaat
    34561 acgtgttgat attgccggta gtgtagaaag tatatacgat attacaaaag atgatttgta
    34621 tctatgttat gaaacgtttt atcatccatc aaatatggtt ttatttgttg ttggtgatgt
    34681 gaatccagaa gaaatatgtc gaatagtaaa acaacacgag gatgcgcgta ataaagttaa
    34741 ccaacccaaa atcgaacgag gactcgttga tgaaccggag gatgttaaag aagcatttgt
    34801 tactgaatct atgaaaattc aatcaccaag actaatgctt ggttttaaaa ataaaccatt
    34861 acaagaagcg cctcaaaaat atgtacaacg tgatttagaa atgtcattat tctttgagtt
    34921 aatttttggg gaagaaacag atttttatca gaatttatta aacgaaggac ttatcgatga
    34981 tacatttggt tatcaatttg tactagagcc gacttatagt ttttcaatcg tgacaagcgc
    35041 tactgaagaa ccagataaat taaaaaaatt attattagat gagttgcgtg ataaaaaagg
    35101 caatttccaa gatgcagaag catttgaact tttgaaaaag caatttatag gtgagttcat
    35161 atcaagttta aactcacctg aatatattgc taatcaatat actaaattgt attttgaagg
    35221 tgttagtgtg tttgacatgt tagacattgt tgaaaatatc acgttagata gtattaacga
    35281 aacgtcatcg ttatatttaa atttagatca gcaagtcgat agtcgtttgg agattaaaaa
    35341 gtaatgaaag cattagtatt aggtggttct ggttcaattg gttctgagat agtcaaacaa
    35401 ttattaactg atggatttga agtttatgtg caatattatc gtactgatat aaatgaatta
    35461 actagcaaat ttaatgatga taaagttcgt tttatacaag cggatttatc tcaaacaatt
    35521 gatattgaca aaacatttgg tgacattaaa tcattagact gtttaatata tgcaagtggt
    35581 cagtctttat atggtgtttt acaagatatg aaagaccatg atattgatgc atgttatcag
    35641 ttaaatgtct tgcaattaat tcgattatgt agatatttcg ttgatgtttt acgtcaaagt
    35701 gacaatggaa gaattattgt aatttcatca atttggggtg agacaggagc tagtatggaa
    35761 actatttatt cgacgatgaa aagtgcacaa ttaggtttcg ttaaggcgct tagtcaagag
    35821 cttgcactaa catcagtgac agtaaatgct atcgcacctg gatttgtagc tggtaatatg
    35881 gcaagtgagt ggcaagaaga tgaacttcaa gcaatgataa atgaattacc acaacagcga
    35941 ttggttttac cgagtgaggt tgctcataca tgcgcctatt tatatcaccc aaatgctaga
    36001 agtgttactg gaactataca gaaagttaat
//
LOCUS       DAHHRR010000014        34966 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.014, whole genome shotgun sequence.
ACCESSION   DAHHRR010000014 DAHHRR010000000
VERSION     DAHHRR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34966
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.014"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..1330
                     /gene="brnQ"
                     /locus_tag="NBI42_000581"
     CDS             <1..1330
                     /gene="brnQ"
                     /locus_tag="NBI42_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD1777026.1"
                     /translation="NMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIANLGFLITAI
                     GLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPRLATTSFEIA
                     FSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLVLLAIILLFA
                     FIHPLGGISDAPISKQYQSNALFNGFLDGYNTLDALASLAFGIIIVATIKKLGIENPT
                     DIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQITQYYLGNYGI
                     VLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLFANVGLTKII
                     MYSVPVLMFLYPLAIALIVLTLFSSKFHHSKTIYQCTIFFTMIAALVDGLKASPEFIS
                     STSFSQTLINFGQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQA"
     gene            1579..2763
                     /gene="rocD"
                     /locus_tag="NBI42_000582"
     CDS             1579..2763
                     /gene="rocD"
                     /locus_tag="NBI42_000582"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCD1777027.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVITKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPNGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEINQIVAAFQDVF
                     KN"
     gene            2802..3833
                     /locus_tag="NBI42_000583"
     CDS             2802..3833
                     /locus_tag="NBI42_000583"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCD1777028.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSLDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAVGSHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLKQSPVYP"
     gene            3845..5086
                     /gene="argJ"
                     /locus_tag="NBI42_000584"
     CDS             3845..5086
                     /gene="argJ"
                     /locus_tag="NBI42_000584"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCD1777029.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASSAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DAQQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHINDANFTTPGAFNEA
                     ILTTDTCTKHIAVSLNIDGKTITIGGSAKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDSDTSTNDMVLVMANQQVEHQILNQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            5102..5872
                     /gene="argB"
                     /locus_tag="NBI42_000585"
     CDS             5102..5872
                     /gene="argB"
                     /locus_tag="NBI42_000585"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCD1777030.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEIKRFDQQYGYVGVPTTLNIDSLSYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIATSLEAPIYVLSNIAGVLINDVVIPQLPLADINQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIEAIYNNDFVGTTILKSLV"
     gene            6158..6637
                     /locus_tag="NBI42_000586"
     CDS             6158..6637
                     /locus_tag="NBI42_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCD1777031.1"
                     /translation="MIIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IVIWVLFHLIIMPIMHTVPAVWDQPFQEHISELFGHVVWMMTIELVRQHFVYRYKLN"
     gene            complement(6966..7610)
                     /locus_tag="NBI42_000587"
     CDS             complement(6966..7610)
                     /locus_tag="NBI42_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCD1777032.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAQQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(7623..14798)
                     /locus_tag="NBI42_000588"
     CDS             complement(7623..14798)
                     /locus_tag="NBI42_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCD1777033.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKFKLFQFADAAFILLDFHVSIFDDSQIDIFLDDLCNAYRDNTVIKNTR
                     QHAHLNKNDDKENQDASHLALDSNYFRLENNSDIHIDSYLPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANQIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHQMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDKLNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDTNYPSDRQAYILKD
                     ATPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEKTTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIVLEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQRPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPEAIVKDDYVLSSAQKRMYLLWKSNHKDTVYNIPFLWRLSSE
                     LNVAQLRQAVQHLIARHEILRTQYIVVDDEVRQRIVADVVADFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDKLQICDIPNDTEELLNWVNTHVNNRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPKKRQGAILEDAKVTAVMSYGIEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGDQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPAQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHQCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYLSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQLVEQDLKQYLNDHLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKQFGHHISMQTLYQYKTVRQIVNYMHQNQQSLVALP
                     DNLSELQNIVMTRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEALQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEMMLSNIEVIVGDFECMDDVILPENMDTVIHA
                     GARTDHFGDNDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTSPYNGRWHMRNIKTN
                     RFSMVMNDLLQLNCIGVSLAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTKLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(15256..16494)
                     /locus_tag="NBI42_000589"
     CDS             complement(15256..16494)
                     /locus_tag="NBI42_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1777034.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGIFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFFLYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGAFLTTLQMGLYMF
                     IVLTIGASMLVAGVFGGQNK"
     gene            complement(16852..17883)
                     /locus_tag="NBI42_000590"
     CDS             complement(16852..17883)
                     /locus_tag="NBI42_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCD1777035.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEATLR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEAFKKRYHQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLETELNS"
     gene            complement(17896..18657)
                     /locus_tag="NBI42_000591"
     CDS             complement(17896..18657)
                     /locus_tag="NBI42_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1777036.1"
                     /translation="MTRLTINKIILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWTFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYVEQFILRRFGE"
     gene            complement(18654..19640)
                     /locus_tag="NBI42_000592"
     CDS             complement(18654..19640)
                     /locus_tag="NBI42_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1777037.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKEPSKKPSKNAQHQQVIKIG
                     YLPITHSANLMMTKKLLSQHNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAM
                     KSKQKGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEEL
                     RKQLKIEPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDV
                     IPDAYCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVL
                     TQSAAWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(19654..20394)
                     /locus_tag="NBI42_000593"
     CDS             complement(19654..20394)
                     /locus_tag="NBI42_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1777038.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLEISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGFQQKISDE
                     EINAQLKLVDLEGRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTESTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYDITASHPRSRN
                     DSHLLKIRNDIMETFALNHHQVEPEYYL"
     gene            complement(20736..21248)
                     /locus_tag="NBI42_000594"
     CDS             complement(20736..21248)
                     /locus_tag="NBI42_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HCD1777039.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKASSLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEAGISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            21427..21630
                     /locus_tag="NBI42_000595"
     CDS             21427..21630
                     /locus_tag="NBI42_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777040.1"
                     /translation="MDKESVVASLARNKKIAVETMTGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKDIDENHLDNAT"
     gene            complement(21689..22648)
                     /locus_tag="NBI42_000596"
     CDS             complement(21689..22648)
                     /locus_tag="NBI42_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD1777041.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNLLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTIILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(23295..24782)
                     /locus_tag="NBI42_000597"
     CDS             complement(23295..24782)
                     /locus_tag="NBI42_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCD1777042.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVEVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGDPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            25209..25592
                     /locus_tag="NBI42_000598"
     CDS             25209..25592
                     /locus_tag="NBI42_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HCD1777043.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRIGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            25599..25925
                     /gene="isdI"
                     /locus_tag="NBI42_000599"
     CDS             25599..25925
                     /gene="isdI"
                     /locus_tag="NBI42_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HCD1777044.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TKDTDEVKILTIWESEDSFNNWLNSDVFNEAHKNVRLKSDHDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(25990..27135)
                     /gene="cap8P"
                     /locus_tag="NBI42_000600"
     CDS             complement(25990..27135)
                     /gene="cap8P"
                     /locus_tag="NBI42_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HCD1777045.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGITANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVILLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRSDKPDEFVPLRHK"
     gene            complement(27212..28474)
                     /gene="cap8O"
                     /locus_tag="NBI42_000601"
     CDS             complement(27212..28474)
                     /gene="cap8O"
                     /locus_tag="NBI42_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HCD1777046.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQKVYEEVLSSGKLKVSTTPEASDVFIVAVPTPNNDDQYRSCDISLVMRALD
                     SILPFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTKACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVDHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            complement(28528..29415)
                     /gene="capN"
                     /locus_tag="NBI42_000602"
     CDS             complement(28528..29415)
                     /gene="capN"
                     /locus_tag="NBI42_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HCD1777047.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KGYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGQVGKSDQIDTQTSMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFVDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMSSMLNKYFNKLSVFRKLFGNLTYSNTLYENNNALEVIPG
                     KMSLVIADIMDETTTKDKA"
     gene            complement(29415..29972)
                     /gene="cap8M"
                     /locus_tag="NBI42_000603"
     CDS             complement(29415..29972)
                     /gene="cap8M"
                     /locus_tag="NBI42_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HCD1777048.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            complement(29983..31188)
                     /gene="cap8L"
                     /locus_tag="NBI42_000604"
     CDS             complement(29983..31188)
                     /gene="cap8L"
                     /locus_tag="NBI42_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HCD1777049.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGSTRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQPQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKPLVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            complement(31222..32460)
                     /gene="cap8K"
                     /locus_tag="NBI42_000605"
     CDS             complement(31222..32460)
                     /gene="cap8K"
                     /locus_tag="NBI42_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8K"
                     /protein_id="HCD1777050.1"
                     /translation="MRLNKFIGDSFLMILSSGIAQVILIITTPIITRLYSPTEFGEFT
                     IFSNIAMILIPIINARYDLLIVNTKNDRSANILSQISFLISLLILLILIPIFAISAWL
                     YPNFILDFIFIIIMLFLVSLTNIFTNYLNKERKYKVLSLINVFRAGSMALLQIIFGLL
                     ALGSLGLIIGFSLSYIAGITLGYKTFKKHFNIVRDKEETKALFLENKNQLVYSTPSIL
                     LNSLSFSVVVFFIGILYTNTEVGIYGMAIRVLGIPVTIISLGLSKIFMQQANDYYIEH
                     GNFRNLLLKFSFILVIVSIILYVPLYLFSEELVNILLGHSWVDAITVIKIVIPLFVIR
                     LIVSTVSLSVIVLQKQQLELILQALFLIGTTATFVISKMLNLTFLNFVSINTIVLIVS
                     YMIFFIALYYFAKNKQFKNS"
     gene            complement(32469..33026)
                     /gene="cap8J"
                     /locus_tag="NBI42_000606"
     CDS             complement(32469..33026)
                     /gene="cap8J"
                     /locus_tag="NBI42_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8J"
                     /protein_id="HCD1777051.1"
                     /translation="MIVKTFMKSKVFRLMNTPLLLFYKKEYLTGYYFENKVAGWLWAW
                     KAVPFKLLGINTSLPFPADITVRMHNPNNIVFDKNDIHIFQSPGTYFNNFSAVIYIGR
                     GVYIAPNVGIITANHNIKNLKSHAPGEDVKIGNYSWIGMNSVILPGVELGEHTIVGAG
                     SVVTKSFPEGNVVIGGNPAKVIKKI"
     gene            complement(33023..34417)
                     /gene="cap8I"
                     /locus_tag="NBI42_000607"
     CDS             complement(33023..34417)
                     /gene="cap8I"
                     /locus_tag="NBI42_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8I"
                     /protein_id="HCD1777052.1"
                     /translation="MNKIYNVTSYVIAILMFPCLMLGDKPLLFLAPISYGVGKLFISF
                     SNNPNFKFSKIVYDVLGFLRLVFIPVMIVFFQDSTIDNLPLGQAYFNQAVIYMSVEFI
                     IGSLFILILSKLFKHEVVSRNSFTLSGSSIYYIVFGLVICGIFVAFPEVRKNISFLII
                     KTDAMGRGTEVTSGLNVLFVMLFQLALALLFLIIAYASYKKYKENPKIIYVVLPLAIG
                     ILNISLIVGERRSYQLYTMVAVLTVVSLLFSKHKRRINIIIISVGIFVLALMTLYKEL
                     YVFNYSSYSEALNSTSVSNLKIVDTLQSYFYGPSNIAASIDYLNYYNGSFKQYLFDNT
                     RAVFGFNFFVDKKQLITSQLFNQLIYGSKQLTGHLISSAGYGIIYFGPLFFYLNLIAN
                     IFFAFLSEYIIRKSHSLEVIFIGTYIYMRLITSIFSHPTPLITLISMILVVYVIAIIP
                     GIIIKKFTKKVGVE"
     gene            complement(34410..>34966)
                     /gene="cap8H"
                     /locus_tag="NBI42_000608"
     CDS             complement(34410..>34966)
                     /gene="cap8H"
                     /locus_tag="NBI42_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8H"
                     /protein_id="HCD1777053.1"
                     /translation="TDGFKKRWPINIGFIGNVRFNEINQKLIKELANDSRFHMQYFGT
                     GSEKLEVFARENFINNITFSGGFDLKETPKYLNKIDILNNLFGNQNIALDTALSIRMY
                     YALFLNKPIITTDDTFTATEANKFGLGFSVNPENLKGIGDELMDWYNNLDVMDINHKR
                     EAYRNDVIENNKQFYQEIGRIFNE"
BASE COUNT        11447 a         6524 c         5207 g        11788 t
ORIGIN      
        1 aaacatgttt ataggttcta tgttatttgg tttattcttt ggtgccggca atcttatctt
       61 cccaatacac ttgggtcaag ctgctggttc taacgttttt atcgctaact taggattttt
      121 aattacagca attggcttac catttctagg tatcattgct attggcattt caaagacatc
      181 tggtttattt gaaattgcat cgcgtgttaa taaaacatat gcttatattt tcacgattgc
      241 cttatatcta gttatcggac catttttcgc cttacctaga ctggcaacta catcatttga
      301 aattgcattt tcgccatttt tatcaccaaa gcaaatcact ttatatttat ttatttttag
      361 cttcgtcttc tttgtgattg catggttttt tgcgagaaag ccatcaagaa ttttagaata
      421 tatcggtaaa tttttaaatc cggtattctt agtattatta gcaattattt tattatttgc
      481 ttttatccat ccattaggtg gcatatctga tgcacctatt agtaaacaat atcaatcaaa
      541 cgccttattt aacggctttt tagatggata caatacctta gatgcgctag cgtcattggc
      601 atttggtatc atcattgttg caacgattaa aaagttaggt atcgaaaatc caactgatat
      661 cgctaaagaa acaattaagt ctggtactat cagtatcatt atgatgggga tcatttatac
      721 cctactagca atcatgggta cattaagtat tggtcatttt aaacttagtg aaaatggtgg
      781 tattgcctta gcgcaaatta cgcaatacta cttaggtaac tatggtatcg tcttgttatc
      841 actcatcgtt atggttgctt gtttaaaaac agctatcggt ttaattacgg cattttcaga
      901 aacatttgaa caccttttcc ctaaaatgaa ttacctagcg attgcaacag ttgtgagctt
      961 tatctcattc ttattcgcga atgttggctt aactaaaatt attatgtact cagtcccagt
     1021 gttaatgttc ttatatccat tagcaattgc cttgattgta ctaacattat ttagcagcaa
     1081 attccatcat tcaaaaacta tttatcaatg taccattttc tttacaatga ttgctgcatt
     1141 agtagatgga ttaaaagcta gtccagagtt catttcaagc acatcattct cacaaacttt
     1201 gattaacttc ggtcaaaaat atttgccatt atcagatatc ggcatgggct gggttgttct
     1261 tagtttaatt ggattcatta tcggcttcat tatttataaa attaagcatc gtaaaattcc
     1321 acaagcataa tactatgcca cagtcatatg ttaaacatat gcttgtggca ttttttattc
     1381 atactacatt aaactgcact cgtatacata tcaatgatta ttcacgaaaa atattagtac
     1441 tttcatttta cgaatcacat taatacacac actactttat ctttatatta ttaaatttac
     1501 atttgacact tatattgaac aactgtaata tattaatatt aattctttaa attgtataaa
     1561 tataaaggag ggagaccgat gaattcaatc attgaattaa ctgattatta tagttctaat
     1621 aattatgcac cgcttaagct tgtaattact aaaggtaaag gtgtcaaagt ttgggatact
     1681 gatggcaaac aatatataga ttgcatttcg ggtttttcag ttgcaaacca aggccattgc
     1741 catccaacaa ttgttaaagc gatgacagaa caagcttcaa agttgtctat catttcacgt
     1801 gtcctttata gtgacaatct cgggaaatgg gaagaaaaaa tttgtcatct tgctaagaaa
     1861 gacaaagtac tcccccttaa ctctggtact gaagctgttg aagcagccat taaaattgct
     1921 agaaaatggg gctctgaagt taaaggcatt actgacggac aagttgaaat catcgctatg
     1981 aataacaatt ttcacggtcg tacacttggc tcattatcac tatctaacca cgacgcatat
     2041 aaagcaggat ttcaccccct acttcaaggc actacaacag tagattttgg agacattgaa
     2101 caattaacac aagctatttc accgaataca gcagcaatta ttttggaacc aattcaaggt
     2161 gaaggtggcg ttaatatacc accgaatgga tatattcaag ctgtgcgtca actatgtgat
     2221 aaacatcaaa tattattgat tgcagatgaa attcaagttg gtcttggtag aactgggaaa
     2281 tggtttgcta tggaatggga gcaagttgtt ccagacattt atattttagg taaggcattg
     2341 ggtggcggct tataccctgt atctgctgta cttgcaaata atgatgtcat gcgtgttcta
     2401 acaccaggaa cacatggttc aacatttggt ggtaaccctt tagccattgc aatatcgacg
     2461 gcagcgcttg atgtacttaa agatgaacaa ctggttgaac gatcagaacg cttaggttca
     2521 tttttattaa aagcgttgct acaacttaaa catcctagta ttaaagaaat tagaggccgt
     2581 ggtttattta taggcataga gcttaacaca gatgctgcac cttttgtgga tcaactgatt
     2641 caacgtggaa tcttatgcaa agacacgcat cgtactatca ttcgattgtc accacctcta
     2701 gtcattgata aagaggaaat caatcaaatt gttgcagctt ttcaagacgt ttttaaaaat
     2761 taacaattaa tcatttatat atgatatagg agggattcat gatgattaaa gtaggtatcg
     2821 ttggcggtag cggttatggc gcaattgaat taattcgatt attacaaaca catcctcatg
     2881 taacgattgc acacatctac tcacattcaa aagtagatga accgttgaaa ttaacatttc
     2941 cacatttaca acatattatg caacatttcg aagcacttac agtggacaat aatgactgtg
     3001 atgtaatttt ctttgcgaca ccagcacccg taagtaaaac atgtatccct cccttagtag
     3061 aaaaaggtat tcatgttatc gatttatctg gcgcatttag aattaagaat cgtgaaatat
     3121 atgaagcata ttacaaagaa actgctgcag cacaagatga tttgaatcat gctatttaca
     3181 gcatttcaga atggcaatct ttagataaca atggaacgaa actcatttct aaccctggct
     3241 gtttccctac agcaacgtta ttagcattac atccacttat tagcgaaaaa attgtagatt
     3301 tgtcatctat cattattgat gctaagaccg gcgtgtcagg tgctggtcgt tcattatcac
     3361 aacgtgttca tttttcagaa atgaatgaaa atctaagcgc ttatgcagtc ggaagccata
     3421 aacacaaacc ggaaatcgag caatatttat ctatcattgc gggtcaagat gtatcagtca
     3481 tatttacacc acatctcgta ccaatgacac gaggtatttt atcaacaata tatgtcaaat
     3541 tatcatctga atatacgact gaatcattac ataaattaat gacctcttat tatgctaatc
     3601 agccatttgt cagaattaga gatattggga cttttccaac cacaaaagaa gtactcggta
     3661 gtaactactg cgatatcggt atctatgtag atgaaacaac gcaaacagca attttagtat
     3721 cagtgattga taaccttgtc aaaggggcaa gtggtcaagc cattcaaaac ttaaatatat
     3781 tatatgattt tgaagttacg actggcctaa aacaatcacc agtttatcca taaggggtgt
     3841 tagaatgaaa catcaagaaa cgacatcaca acaatataac ttttcaatta ttaaacatgg
     3901 cgatatcagt acacctcaag gcttcacggc tggtggtatg cacatcggtt tacgcgctaa
     3961 caaaaaagac tttgggtgga tttactcatc gtctttagca agttcagctg ccgtatatac
     4021 tttaaaccaa tttaaagctg caccacttat tgtcactgaa gacactttac aaaagtctaa
     4081 agggaaatta caagcacttg ttgttaattc agctaatgca aattcttgca caggtcagca
     4141 aggtatcgat gatgcacaac aaactcaaac atgggttgct caacaacttc aaataccatc
     4201 tgagcatgtt gctgttgctt caactggggt cattggagaa tatttgccaa tggataaaat
     4261 taaaactggt acagaacata ttaatgatgc taattttaca acaccaggtg cgtttaacga
     4321 ggcaatttta acaactgata catgtacaaa gcatatcgct gtatcactaa atattgatgg
     4381 taaaaccatt acaattggtg gcagtgctaa aggatcaggt atgattcacc caaatatggc
     4441 taccatgctt gcttttataa caaccgatgc atcaattgaa tcgaatacac ttcatcaatt
     4501 attaaaatct tcgactgacc atacatttaa tatgattact gttgatagcg atacaagtac
     4561 aaatgacatg gtattagtca tggcaaatca gcaagttgaa catcaaatac ttaatcaaga
     4621 ccatccacag tgggaaacat ttgttgatgc attcaatttt gtctgtacat ttttagctaa
     4681 agctatagcc agagatggcg aaggcgcaac aaagttaatc agtgtcaatg tttcaggtgc
     4741 taaatcaata tcagacgcaa gaaaaatcgg taaaactatc gtaagttcaa atctagtaaa
     4801 atcagctatt tttggcgaag atgccaattt tggtcgaatc attacagcta ttggctacag
     4861 cggatgtgaa attgatccta actgcacata tgttcaactg aaccaaatac ctgtcgtgga
     4921 taaaggcatg gctgtactat ttgatgagca agctatgtcg aatacattaa ctcatgaaaa
     4981 tgtcacaatt gacgttcagc ttggtttagg taacgctgca gcgactgcat acggttgtga
     5041 tttatcctat gattatgtgc gtattaacgc atcatatcga acataaggtg gtgttggtta
     5101 gatgaaattt attgtcatta aaattggtgg cagtacacta agtgacatgc atccatcaat
     5161 tattaacaac attaagcatt tacgatcaaa caacatctac cccattatcg ttcatggcgg
     5221 tggcccattt attaatgaag cattatcaaa ccagcaaatc gagccacact ttgttaatgg
     5281 cctaagagtg actgataaag caactatgac tattactaaa cacacgctca ttgcagacgt
     5341 taacactgca ttagtagctc aatttaacca gcaccaatgt tctgcaatag gcttatgtgg
     5401 tttggatgct caattatttg aaattaaacg ttttgatcaa caatatggat atgtcggggt
     5461 tccaaccact ttaaatatag actcattgtc ttatttatgt actaaatttg tacctatcat
     5521 caattcgatt ggtttcaata accatgatgg agaattttac aatataaatg cagacacact
     5581 cgcctatttt attgcaacat cattggaagc acccatttat gtactaagca atattgctgg
     5641 cgtgctcatc aacgatgttg ttatacctca actaccctta gccgatatta atcagtatat
     5701 tgagcatggt gatatttatg gaggtatgat tcccaaagtg cttgatgcta aaaatgctat
     5761 tgaaaatggc tgtcctaaag ttatcattgc atcaggaaac aagccaaata ttatcgaagc
     5821 tatttacaat aatgattttg ttgggacaac gatactaaaa tcattagtat gactttgaat
     5881 aagaccactc aatcttgtat ttttcggctt cgaaacatag ttgtacctaa tttgtttaat
     5941 gattatgcag cgtcaccttt tcacctatct catttcacaa aagaaatctt agaatatagt
     6001 atgatacaat ttgagatgaa aaacactatt agtgattttc aaatttgtaa cttgaaatat
     6061 tttatatttc aaaagttttt ttattgtgat tgttttcaca aaatactata atgaggatag
     6121 aaaaaagaga ggggtcctta agttgacgaa acgacaaatg ataatattca tttatgccgg
     6181 aatcattggt ggcttgttgt ctggaattgt aaaattaggt tgggaggtca tgtttccacc
     6241 tcgtacacca gaacgaaatg caacaaaccc acctcaagag ttattgcaac aattaggatt
     6301 tagtagtgaa tttacacatc agacgtatac attttcaaat atggaattac cttgggtaag
     6361 cttcattgtt cactttagtt tttctatcgt cattgcaatt atttattgca tattagttaa
     6421 aaaatacgct tacttagcaa tgggacaagg tgctgtattc ggtattgtaa tatgggtgct
     6481 attccacctt atcattatgc cgattatgca tactgtacct gctgtgtggg atcaaccatt
     6541 ccaagagcac atatcagaat tatttggaca tgtcgtctgg atgatgacta ttgaattagt
     6601 acgacaacat tttgtctatc gctataaatt aaattaattc atataaacat cgtaacgtga
     6661 gttttaaatc atcgtttgag tatgatgatt gatgctcacg ttattttatt aactgacatg
     6721 atatgattcc agccaactta cgtgagcatt aaagtctcaa atgcgtcgta acaagccatt
     6781 atttttggca atttcaatat tgctcagtat attttcacct tatcacttac tataatctca
     6841 tcatgatttt gaatgatgcc atcgtgtatt cacctttcat ttttccaata aaaaaacatc
     6901 taacagtaag catttaggca gtatagttta aaatcactgc gcaatgatac tgttagacgc
     6961 catgtttaac tactcaataa ctgaaataca gacacttttt tataacccca gggtgcctgt
     7021 cctaagaaac atacctgtac cataaactga tcaaaaataa attgtttgaa cttcacttca
     7081 cgtgattgat aaaagtgtga ttgcgtcata tcataaatgt ccaacccttt gattaaacct
     7141 tcaccaatca attttgtaaa actttctttt tgtgtccata tttgataaaa atcatttaaa
     7201 ctacatattt gctgtgcttc atttgtagag aaacacgtca ctaatgtacg ccagtctaaa
     7261 cgttgtgata tcttttcgat atcaatacca actggttctt tatcgacaac acacacgata
     7321 taaggataac tatatgataa gctcacatag atgggctgtc catcacgatt gtgttgaact
     7381 atatctgcct tacctcgtgg cgaaatgtga taatgccatt catgtggtaa taaccctgtg
     7441 tcatgttgaa ttccatattg cactaaaata tctcccaatc tgtgcatgag tttatcttga
     7501 ttgtatctat agttgactgt acgcggtttt ttatatgacc aacgactttg tgatattaat
     7561 tcttcaatac ttttcaagtt actctgtaat tgcattacaa atactgtcat aactttccct
     7621 acttacttat tgaatattgt tttgatatat tgcgcccaat gatacagcca attgttagtt
     7681 atcgttggcc atttttcact gatgtgattc attattttta atgttaattt tgtatctatc
     7741 attgctagtt gttgttcacg gtcaacacta gttaatccaa tcgtttcgta catgtcttgt
     7801 ttctgtaaaa tttcattaaa tgattcatcg ctgacgagtt caatttcttt gcgcttaacg
     7861 cattctaaca aagatttcac cggcatttta ttaggtgata gcacatggta aatgatttgt
     7921 ggtgtgttga cctgtgctaa tgcgacaatt tgtcttgcag tcgtatccac aaaagaaaaa
     7981 tctacaggca tttcagcgag gctaaccccg atacaattca gttgtaacaa atcattcatt
     8041 accattgaaa aacggttagt ctttatattt ctcatatgcc atcttccatt gtaaggactc
     8101 gtcaaattac caacacgtac aatccgacca tctaagccat tatttacagc ttctaatact
     8161 tttaattcac tataaaattt actccgtgta tatggtgatg ttagtagttg tcctttatag
     8221 acatccgctt ctgaaaatgt cacatcttct gtgtctatat caaaataagt tcccacactt
     8281 atcgtagaca catatattaa ccttgcatga tgttgttgtg ccaaacgtat gacatcaaca
     8341 gtaccttgaa catttacttt ttcaaattca tcattatcac caaaatgatc tgtacgagca
     8401 cctgcatgaa taaccgtatc catgttttct ggtaaaataa catcatccat acactcaaaa
     8461 tcaccaacaa tgacttcaat gtttgataac atcatttcaa ccgtctcttc tgaaaaataa
     8521 tcatttaaat tcgtcatcaa cttataccat gctatttcct cattatcagc acgtataaaa
     8581 caataaatgc gatgactgta tccttgtagt gcttcaatca gataagcacc taaaaaccct
     8641 gtagcaccag tcaatagtgt atttcctaga ggtcgatgac ttagactatc ctctaaaata
     8701 cccaaattat aacgagtcat aactatgttt tgtaattccg aaagattatc cggtaatgca
     8761 actaatgatt gttgattttg gtgcatataa ttaacaatct gtcgcacggt tttatattga
     8821 tataatgtct gcattgaaat atgatggcca aattgtttta aatgcgagac aactaacatc
     8881 gcctctaatg agttaccacc aagttcaaag aaatcatcgt caacaccgac atcattttgt
     8941 ttcaatacct ctccgaatac atcaacaaat gtctgctcaa tttcattaga tggttcgcta
     9001 tacactttat tagactgttg tataggtgat ggatttggca aacgcgtagt atccaccttg
     9061 tcattcgtgg ttaatggcat acaatcgata tgcgttatag tcttaggaat catatactta
     9121 ggcagatgat catttaaata ttgcttcaaa tcctgctcca ctagttgctc accgacataa
     9181 taagcattca atatatcatg cgtatcaaag tgacttactg tgacaacaca atcagatata
     9241 ccacgaatag ctaatattgc attttcaatt tcatcaagtt caatacggta cccgttaact
     9301 ttcacttgtt tatctattct tcctaaaaat tcaatttgac catcagatgt ataacgtgct
     9361 aaatcaccac taagatacaa ctttccttta ccaaatggat tattttgcca tttatcagcc
     9421 attaattctg gacggttaat atatcctatc gctaaactat cacctgcaat acacaactcg
     9481 cctggcatac caataccgca taacaaacca tctgacataa tatacacttg gatgttagat
     9541 aagggtttgc caattggaat cgtctcaggt atcaaatcac cacattgatg tgaccaatac
     9601 gatgtgatga ctgttgactc agatggtcca taggcattga aatacgtgcc acaatgcttc
     9661 tcaatatatt taacaaagga tgccgtacta gttgccccac ctgtaatcaa tttttcaata
     9721 taaaagtctt ccataacact acacatctgt aatggaatcg acgccaccgt cacacgatgc
     9781 ttattaatga gttgttgtaa ctgtgctgga ttaacacgtt cctctctatc tggaatcaca
     9841 agcgtatgac catttaacaa acaacaataa atctccataa ctgatgcatc aaaaacaata
     9901 tttgcatgtt gcaaaaatac ttcattgtcg cctaattgca attcagttga ccatgcatgc
     9961 actaaattca acaaatttcg ttgtcgtatg gcaacccctt taggcattcc ggtcgtacca
    10021 gatgtgtaaa tagcatacat ctcattatct aacatcgctg tgttttcaag ttgatcgcca
    10081 tgtaaatcat catattgttc attttccttt gattcaacaa aacctttagc attttccaat
    10141 tgaatgactg gtaatgtcgt ttcaatttca atgccgtaag acatgactgc agttacttta
    10201 gcatcctcca aaattgcacc ttgtcgtttt ttcggaaaat caatatcgat aggtatataa
    10261 gatgcaccta ctttaactgt cgccaacatc gccgcaatca tttcaaaact acgttctgta
    10321 aacaaggcaa cccgttgacc attgcccaca ccatttgata ggagcatgtg cgcaatggca
    10381 tccacatagt tgcgtaatgt ttcatatgtc attgtcaaat cattcatgac tagcgcaaca
    10441 tgattacctt gtcgtgagac aacttcatta aagtaactta tgatagattt atttcccggg
    10501 acattaagca ttcgattgtt aacatgcgta ttgacccaat ttagaagttc ctccgtgtcg
    10561 tttggtatat cacaaatttg tagtttatct tgatgcttca aaatataatc aatcataatc
    10621 atacattgat tacccatgtg acgaactgtt tctgagtgat ataaatcggt attatactcg
    10681 atattgattg tatagtcatc gcgatcttct tcaatgatga aagataaatc aaatttcgcc
    10741 gtcactgatt taggttgaat atgtgttaat ttactatgcc caaaatgagc atgattcgtt
    10801 tcattgtttt gtagtactaa catgacatca aataatggat tccgtgaggc atcatgtgat
    10861 tgatctaagt catttactaa acattcgaat gggtattctt gatgctcgta tgcctccaaa
    10921 ctcatttcct taacttcttg taaaaactgt gtccacattt tatcaggtga cggttgccct
    10981 ctatatacca acgtattagc aaacatgcct agcatttgct ccgtgccttt atgcatacgc
    11041 gcactcatta cactaccgac aacaacatca tcttttcgag catatctgct taacaacgtc
    11101 atgaccacac tcataaagaa cataaaatca gtaatttgat gcttttctac atacttttga
    11161 agtagctgtc tcatttgttg attcattgta aatgacatca ttgctccatt tgtcgtttta
    11221 atatttggtc taacatagtc tgtcggtaag cttaaaatag gtacttcatc tttgaattga
    11281 gataaccaat attgtctatg tttcgtcata tcacgatgcg acatccactc actatagtct
    11341 ttatattgta atttaagtgg taacaataat ttatgttgat ataatgcgtt aagatcattc
    11401 attaattgta tattgctcat accatcatta atgatatgat gtgtatctat aaacaggtat
    11461 gcatgtaatg gacttctaat gtatctcact ctaatttgac ttggcttttc caaattaaaa
    11521 ggtgccacaa attggcgcat gatttcttgt tcatccgtaa aatgcgtgtt aacttcttca
    11581 aagtcagcta caacatctgc cacaatacgt tgtcgaacct catcatctac aacaatatat
    11641 tgtgttcgta gaatctcatg tcgcgctatc aaatgctgaa ctgcttgtcg caattgagct
    11701 acattaagtt ctgatgataa ccgccataaa aaaggtatgt tatacaccgt atctttatgg
    11761 tttgatttcc ataataaata catacgcttt tgtgcagagc tcaacacata atcatcttta
    11821 actatagctt caggaatcac ttcatagttt tgttcttgaa ccttagcaat tgcttgtgct
    11881 agttcaaata cagttggctt ttgtaataaa tcaccaattt gtaatcgttt cccagtagat
    11941 gcctctatcc gattcaccac taacgttgct tttaatgaat ggccgcctaa ttcaaagaaa
    12001 ttatcatgaa tacctacttg attcacatgt aaaatatctg caaaaatttg gcatagcaag
    12061 tgttcggtat ctgtactcgg tgctacatag gcatccgtat cgacatagtc catgataggc
    12121 aatgccttct tatctaattt cccattaata gtaataggaa tttgctcaat atgcataaaa
    12181 ttaactggta tcatgtactc cggtaaggtc atacgtaatt gtgatttaat cttattatgt
    12241 gataatgtat gcatcgcttc ataataagca acgatatact ggtcttgatc atgattttga
    12301 acaataacaa ctgctttatt aataccttgt atacgctcga gcgcatgctc aacctctgac
    12361 aactcaatcc taaatcctcg aatcttaact tgtttgtcct ttctatataa ataatctatg
    12421 ttgccatcgg gtaatagacg aacgatatca ccacttctat acatcagctg atttatattt
    12481 gaatctttga taaatttatc tgctgtcaat tctggctgat ttaaataacc tgcagctaac
    12541 ccaaagccac ttgtacataa ttctccagga ataccaacgc cacagcgacg ctcgccttgc
    12601 atgatataaa catgagtacc aagaatcggt ttaccaatag gaatacgatt tggaactttg
    12661 ttaggtatat tatacgtcgt tgtaaatgtt gtattttcag ttggtccata accattaata
    12721 atttgaggat gcttcggtct ttgattaagc aaatctaccc acttagcatt caatacttct
    12781 ccaccaatta ataaatactt taacggtacc aatacttcta ttcgttcact agcaatctga
    12841 ttaaataatg aggaggttaa ccacatagta ttaacgtcat tttcattgat taattgttct
    12901 aacactattg gatttaataa ttgttctttt ttagcaacaa tcagctttcc accattgagc
    12961 aatgcaccat atatttcaaa tgttgcagca tcaaaggcta tagttcctga taacaaaatc
    13021 gtcgtctttt catttaatgg tacataatga ttttgatgga ccaagcgaac aatacctcgg
    13081 tgtggaatta gtgtcccttt agggttacca gttgtccccg acgtgtaaat aacatacgca
    13141 tgatcttcta acgtgttaca tttagaaaga ttatcaatat ttttccacgc tatcttgttc
    13201 aaatcaatgt gattaatatt ttgtttacca ttttcatata aagcttggta cgttattaca
    13261 actttaggcg ttgcatcttt taaaatgtag gcctgacgat cacttggata gttcgtatca
    13321 attggcacgt aagccccacc agctttcaac acacctatca tcgctattat catctcaata
    13381 cttttttcag ctatgacagc gacacgatca ttaggttcaa caccgtactg gtttctcaaa
    13441 cggtgtgcta aatcattcgc gcgtgcattc aatgtttgat atgttataaa cactccgtca
    13501 aattgcacag cgacatgatt cggcgttgct tcaacttgtt gctcaaataa ggtaacaact
    13561 gtttgcgcat catctatctc aggcaaactt aaattgatat cgtcatataa ttgaatatca
    13621 cgttctgtca tcaaattaag tttatctaca gttgttcgtt tatttccatt ttcttcagta
    13681 atttgcaaat aaatattacg aactaagtca ctcagcgtct cgattgagag caaatcatat
    13741 gctgcactgt tatatataat tttgaacccg tgttgatgtg gaaaaatttc aatatccgct
    13801 aaagatgtat gtgcatcatg tatttgatgt acatcctcaa taacatcatt acaacaagac
    13861 atcatttgat gataacaatg aaaaatagtc tctaatgaaa gcgaagactt cgcgcactgt
    13921 aattgcgaca tattttgcaa cacacatttg ttaaaatctg ttgtaaaacg ttgacataca
    13981 tcttttgcat cgattgttaa cgttaatggc acaatatttc cgtgtaaatc atttggtaaa
    14041 tgtgatggta catgtatacc taatgtgaca tcatgttgtt gactcattat ttgattagct
    14101 aaatacacac taacagccaa cgatgccata tctattgatg tcatatcatc aattaaatac
    14161 gtttgatata aagcttgctc aaatggatgc ttaattggta aataactatc aatgtggatg
    14221 tcagagttat tctctaaccg aaaatagttt gagtctaatg ctaaatgcga tgcatcttga
    14281 ttctctttat catcattttt attcaaatgt gcatgctgtc gagtattttt aataacagta
    14341 ttgtcacgat atgcattgca taaatcatca agaaaaatat caatttgact atcatcgaaa
    14401 atggacacat gaaaatctaa tagtataaat gcagcatcag cgaactgaaa caatttaaat
    14461 ttgaataaag gtgaatcatt aaaatggtaa gtacttaatt cttgcttaaa aaaagcttct
    14521 aaatcatagt ttgcggaaga agatggaact tgttttatct caataaaagg cagaaattca
    14581 tgaagtatca ttgttaaatt gtcatcggta gtaacatcaa aaaaatgtct tatagatgca
    14641 tgttgttgca caattgtcga taatgcatac atcattttag tagcttcaac atttttagcg
    14701 agtttaaccc aatacgcatt acggtgtgtc gttgattctg tattattttt gtatatacga
    14761 aaatattcct gttgaaatct caaattaccc ataatcataa aaagtccttc tttcatatca
    14821 taatactcat tacttattga aattgcatga tgatatgata accgacgaaa tgttaattaa
    14881 ctcgttatgt aatgattaat ataaaacacc attcgcaaca tatgagcgat atattctacc
    14941 ctaaaataca tcttgtatca tcgttacaat tggtatactt ttcaatgtaa attacataca
    15001 tcttcgataa atagcacatt acaactcgtt aatcactttc tcttgttcac atctcattgc
    15061 aaattcaata ttgttgttac aaaatatcca tgaagcaagt ttatattaaa caaacaactc
    15121 gcataaaaca attgttatcc ttaaatttta acaaattctt aataaattta tctctatttt
    15181 aattatgacc aaattaatag gttttcaata taaaaagatg catagaataa atatttaaat
    15241 aaattcaatt tgtattcact tgttttgtcc cccgaataca ccagcaacaa gcatgctagc
    15301 accaattgtt aaaacgataa acatatacag tcccatttgt aatgtcgtta agaaagcacc
    15361 caacacaatc cctaacctag ccagtgtttc tgaaaaatga atacctaatg cattaactgc
    15421 actatatgtt cctcttttag ctttaggaat aattttaaag cgttgttctg aaactatagg
    15481 cgaataaata atttcaccta cagtcgcaat tatcataaaa acaactaata agccaaactg
    15541 attaagatag gttagaccac tatagccaac aatataaatc agcaaaccag tgattaaagc
    15601 ttttttaaaa tctattttca atacaacttt cgagattgaa tacgtgagta aaatgacgac
    15661 gaccgtatta atcattagca agattgctaa catcttagca cctgtaatat catatgaacc
    15721 tatacttatt gtttcaaact gatcctttag tctaatagca atatatgagg agattgaaaa
    15781 ttcacccatc atgatgatac tgaaccccga aatcaataac atataattac ggtctttcaa
    15841 aactaattta taactgcgaa atatattcat tatttgtaat ttttgataac gacttgcatg
    15901 cctcttgtca tcactctgct ttacttgatt tcggtcttga ggtaaccaaa tatataaaaa
    15961 aaagagtaca attaaaaata tacaagctgc tattaagaaa agtagtaaca tactgtagcc
    16021 atacatcaag ccacctaaca atgccccaat agctaccgat aagttcgtca tccaatagct
    16081 aatcttgtaa atataatgtt ccacgtcttc ggtaattgca tccataatta atgtgtccat
    16141 aactggaaat tgtaatcccc aaacgattgt aaatatggca tatgcaacac aaaaaccaat
    16201 aatttgccac aattgatgtg acccaaatat gcccatgaac acaagcatta tcaccatcgt
    16261 cgcttgataa ataagtacta gcaacttttt cggaaatatc tcaataaggt aaccagatat
    16321 aatggacaat ggaaatttta gaaccactaa accaacaaga tatataccga caattgattg
    16381 acttaacata tctgttaaat atagtgctat aaacggtata aatgctgtcg taataattag
    16441 ctgtaaaaaa ttgctaatca atcgtacttt caaagtcgta cttaagcgtt tcattaataa
    16501 ctacacccct atccacatat atacacacta ccatttatat aaaatctatt attagattat
    16561 acgttgaaac aaagaatttt tagttataca aagttattta acaaacaaat gcatcgactt
    16621 tacaatgcat cttgcatata atacctcaaa aaaattagca ctatatgact atatattatt
    16681 ccaaatgaat gaaatttatc aataattgcg taagtgaaca catatgtgcc cgaacgttat
    16741 attataattc aggcactaaa ttttatatgc gtaatcgcag taaaaaacta ctaccatcta
    16801 taaagcggta gtagttttcg acgaatagaa taatgcacca tcttaattca gttagctatt
    16861 caactctgtt tcaagtttac ctaaatgtct taatgttggt gtcaatgctg cgaagaagaa
    16921 tccttctttt aacttgcgaa cttgtggtga atatggtgta tatgctcttg agccaccatt
    16981 gacaacagaa gcttgattca catctagcaa taaatagccg atgtccttct tcaatgatat
    17041 taattcattt aaatgtgaag ttaagttacc gtcatctaat attgcataat attcctctct
    17101 aagttgatga taacgttttt taaaagcttc aacatcatac tctaaatatt gatttattcc
    17161 gttttgcgca tttgaaaatg catcaattaa ctctaaagaa cttttaattg agcctaatcc
    17221 tattggaatt tggtaagcaa taaattgcgg gcgaatagtt gccgcaaact gcttcgcatc
    17281 atgcgtgata atttgtgatt gtggcactac gacttgattc aatgtaatct gatacgttgc
    17341 tgagccgttt acccctaaaa aatttgtttt ttcaacaaga gtgatcccat cttgattggc
    17401 acgtagaatg aacatgacaa attcatctga tgattcatgc ttcgaaatcg caccaaaata
    17461 atgatcttct tgaatattac ttacagctgg catacgtcca ctgacaacca attgtccatc
    17521 aacataagta tgttcaaggt ttaatttttc taaatcatta aatgacttca tcggattaga
    17581 caatccggta gcacctaata tttctccaga taacaattgc tgttgtaagt cattatttaa
    17641 atgtggctgc gtggcatttt ctaaatacgt tgaaaaagcc aattggcacc ataaacaaaa
    17701 tcctgttgtc aaacaagact gcgatacagc ttcgattact tcagcatttt ttctcagtgt
    17761 cgcttcacca aaatagccat ctacaaataa ttgttggata aatgttctcg gataatacgt
    17821 cccttcatca acttctacta aatgaggatc taattgttct ttgataagcg tttctaaagt
    17881 catcatcatc tctccttatt caccaaatct tctaagtata aactgctcaa cataactaat
    17941 gaatcgatca ataataaaac caaataatcc gataaagaat attgctgcta aaacatcttc
    18001 taagttcaac atatttcgtg catcaacgat taaaaaacct aatcccgatt gtgcaccaat
    18061 catttcacca gaaactaaaa atatccaact tgttcctacc gccatatgta tcccagccat
    18121 gatttgttta aatgccccgg gaaataatat attgcgatac aatgaccacc cagttaaatt
    18181 taaatttgcc gctattttta aatattgagg ttcaatgtct ctaacgcctt taatggtatt
    18241 gaacacaatt gggaaaaaag cagcgataaa aataatcgca atcgctggca aactaccaat
    18301 accaaaccat agaacaacaa atggtgccca tgctatcgga gatatcggcc taatcaattg
    18361 aaatagcggt tcgatagcgt tgtatagcca acgattcctt ccaagcaaga agcccaacgg
    18421 aatagcaacc aacaatgcga caacaaagcc cgctacaaat ctccataaac taattgctaa
    18481 atgttggaaa atttctccag taacaatgaa agtccatatg ctttttccta caagagcagg
    18541 accaggtaac aatacaggtt ggtaatgccc aataataatg accatttccc aaatgcctaa
    18601 gaaaataata aatgtgataa taggtaatat aattttatta attgtgagac gtgtcatgaa
    18661 cgtgatgcct ccttatacaa tgacggttca acaaagtcat catatgcagg tggattaaac
    18721 aaatgatgtt gttttaccaa tgtcgtaatt tcttgatagc cggatggctt aattgttaaa
    18781 tcaccatagg atgtccatgc cgctgactgt gttaaaacgt cacgactttg tttaaaatga
    18841 tgcgtcataa tgtctacact ttgcttgcga tcattcattt taaagccaga ctttttataa
    18901 tcttgtacaa atgcttgcgc tacatccttg tgttgatcaa gcaattcgcc tctcagtact
    18961 agcacacagc aatacgcatc aggtataacg tcatcaccat gtttcaaagt cttacctttg
    19021 cctaactttt cacccagtgc accgaatggt tcggctacag aataccctgt aattctgtgt
    19081 tcacttaatg cggctggcat ctccgctggc gacatttcat gatagctgaa atgacccggt
    19141 tcaattttca attgtttacg taattcctca agtaaaagat aatgtgttga ataacgatgc
    19201 ggtataccaa aatggtaatc atcgccatta ttattaaatt catttaagtg catacctttt
    19261 tgtcccataa tgacattgcc ttcatgatga cccaatgcca cagcctttat attcgagccc
    19321 ttctgctttg atttcatcgc gagctctatt aaagttgatg caccatcaat acgaccactg
    19381 tttaatgcgt ccattaaatc tggccaatta ttgaatttaa ctaattctaa tttatatttc
    19441 ggatgattgt gttgtgataa taatttttta gtcatcatca aattagctga atgtgtaatc
    19501 ggcaaatatc caattttaat cacttgctga tgttgagcat ttttagacgg ttttttagac
    19561 ggttctttag acgtcctttg ccaatcacat cctgtaatta taaagattcc aatgatgacg
    19621 attatgctta accttttcat cgtcactcac tccttataaa taatattcag gttcaacttg
    19681 atgatgattc aatgcaaatg tttccataat atcattacga atcttaagta ggtggctatc
    19741 attgcgactg cgtggatgtg atgctgtaat atcatattgc gaaataatat tgcatccttc
    19801 tcctaataga acaatgcggt cggaaagata aatagcttca tcaatatcgt gagtaactaa
    19861 gataatagtt gattccgttt tgtgttttag ttgcactagt tgatcctgaa gtttatagcg
    19921 tgtaaacgca tctaatgcac ctaatggctc atccatcaat ataacgttag gcttatgtac
    19981 atgcgctcga cataatgcta cacgttgttt catacctccg gacagttgct cgggaaaatg
    20041 ctttcctctg ccttctaaat caactaattt aagctgtgcg ttaatctctt catcactaat
    20101 tttctgttgg aatccaatcc taatgttgtc attaatcgtt ttccatggca gcaaattatg
    20161 atgttgaaat agcattaaac aatctggaga cggctgttgt ttaatttcgt tatcaataat
    20221 gacacgacca gatgatggat gaataaatcc accgataata ttgagtaaag tagacttccc
    20281 gcaaccactt ttccctataa aagtgactat ttctcccttg ctaatctcca aattaaagtt
    20341 atgaattact ttatgtgatc caaagtgatg ttgtaattgt tgaattttaa tcatgacaag
    20401 ccccctttta atccatactg ttttaatagg aattaagtgt atatctaaag ggctatgcca
    20461 aacacagccc ttaaatcgtg aattgttata tctacagatg attatggtct agtgcgaacg
    20521 ctcttataac ataagcgtta ttcaattaac gacgttatat taatttcagc tctacaaaat
    20581 ttcaataaaa tgattgtttt agccaataat tgcatatagt tcattagttt ctccatatca
    20641 actacttaca tttatagcat tgagctattc tgattaactg ccatctaaat ccacgccaaa
    20701 cctatataca tatatctcat caacttgttg tcttattaaa gtttttcgat gccatcaatc
    20761 ggtgtatcaa ctgctttgcg cgcgcgacgt accgcttctt catcaggtgc gttgtataaa
    20821 caaatacatt tagacatatc ttcacataca tatgtgcgtt taaattcaac ttctggcact
    20881 tcttcatagt gaacagaatt tttcttttta cgtgctaaat attgatccat cgtaatgcct
    20941 tccggaatgt tccaagttac aaggtaatca acatgtgcat caccattttt aacttcatct
    21001 aaatctttac caactaagcg tacttctttg actaattgaa tgctaatacc tgcttctgtt
    21061 aaaaattgtt tagcttctgc ttcatcattt gcttccacaa taaaataacc atgagttaaa
    21121 ttttcagtag cttgtacttc aattaatgtt ggaattgtct tcgtagatag tgatgatgcc
    21181 tttgcttcta gttcttcttt cgttgatgca aaatcaagat tgttagcttc taataaaaat
    21241 aatgtcatta ataagacctc ctataacaat ggttgaaaaa atattactat aattcttatc
    21301 ggattacaag gatattgagc gattttttta aatatttaga taaaattctg tgaaatatat
    21361 tttcaaagag tataaaaatc atgttagggt ataagtaaat aaacatatat atagggggct
    21421 atcattatgg ataaagaatc cgtcgtagca agtttagcaa gaaacaaaaa gattgctgta
    21481 gaaacaatga ccggtcaaag gtacatcata gaacgaattt tacatacaaa tgatgaaaaa
    21541 catattcata ttttgaaacc taaagatgtc gtgttagatg tagatagcat caaagacatc
    21601 gacgaaaatc atttagataa tgcaacataa tccagaaaaa catgcgccta tgacatagat
    21661 taatgtttta ggcgctacaa tgttttattt atgttaattt gtcgaagtat ggctcatcta
    21721 atggattcaa ttgcaagttt tcaatttgca acgttgaatg tgcaattcca tatttttctt
    21781 tcaacaatga agatacttga tcaatcgctt gataatcatc gccctcatat tttttatcta
    21841 acacaacatg ggcacttaat gaataatgct ctgttgtaat actccacaaa tgaaattcat
    21901 gtacatctaa tatgccatct atgtttttaa tatctgccat aatttgatca gtatccaaat
    21961 gttgaggcac actttccatt aaaattaacc acgcattacg cgtaatttta taaccaccac
    22021 gtaaaatgat gagtgaaatt acaatactaa tgattgggtc gatgatgcgc catcctgtaa
    22081 agtaaatcaa tacaactgca acgatgacac caatagagtt caataagtct cccatgaaat
    22141 gccataatgc actttgaata ttgatattgt cttcttgttt taaagacctt acaaggataa
    22201 tagtcaaaat aatattgacg agtaaaccaa tactagcaat cataaacata atgccacttt
    22261 caattggttg cggataaata atacgtacaa tagcttcata taaaatccag attgaaatga
    22321 caattaatgc taaaccattt aaaaatgccg ctaatatctc aaatcttaaa tatccgaatg
    22381 tgtagcgtgc agtcggcttc ttacttgcaa aataaatggc caacattgac aaaccaagtg
    22441 ctaatacatc actaagcata tgaaatgaat ctgacagtaa tgccaataaa ttagatacta
    22501 aacctccgac aaattcaatc accgtaaata acaatgtgat gataagtgat gcccatagcg
    22561 tctttttaga actttgttga attttttgat gattgacatg atgaaaatat ttgtatctat
    22621 ttatcgtatt cgcctttttc ccgttcatag tttgccactc ctaaataaat gcatcgtatt
    22681 taatattgtc gcttttaaaa ataattcttt atagcttctc agattaatat taatgcttcc
    22741 gtaattaacc atgatttata catcatcgtt ctttacatga caactcatta atctttaccc
    22801 tttatacctt tatgtaatat caccttggaa aaacttttac agcacactcg cgctttgctt
    22861 tatccctatc atatagaaaa ttttaataat gtacgagatt attgtgttag ttacataaaa
    22921 atgacaaaaa agtatctcaa atttattatt tagtttcatt aagttcaaaa aatcgcaata
    22981 gctttaatga ttgttccaca tattaaaaca aacgatgact ttgcatatag aacttcacat
    23041 ttctatatca ccacaattaa taaacaactc tgtccttata atagccacat cgcaaattag
    23101 cataattaaa aacagcagta agatgatttg caattaaaaa ccatcctact gctattattt
    23161 tagacattct atcctaatat cattatgcat gttcctaaat attttgtttc gcttaaaatc
    23221 taaacaactc cgtaaacatg ctaagactta ccgctagact gaccgacaaa cacgcctcaa
    23281 aaacgatatt tattctagta caaacctttt aaagcattac ttgtatcaat ataaatattt
    23341 ttaacttgtt gatagttact taacgcacct ttataagttt ctcgaccgat acctgatttt
    23401 ttataaccac caaatggtgc gccttctggt acttggttgt aagtgttaat ccaaatacgt
    23461 cctgtacgta cagctttagc aatatttaat gcacgtgtga tattttgaga aaatacgccg
    23521 ccagctaaac cgtactctga atcattagct atatcaattg cttcttgatc gtccttcact
    23581 ttaatcactg ttaaaactgg tccaaatatt tcttcttgcg ctaacttatg atgattatct
    23641 ggcacagcaa ttaacgttgg ttcaaagaag aatcctttat ctaacccatt ttcagttaaa
    23701 cgatgaccac ctgctaaaat ttgtgcatct gattcttttg ctgcatcaat atatgattga
    23761 attttatcta attgatcctt accagtttga ctacccattt gtgtagcttc atcttgtggg
    23821 tctccaactt taatatttga aaatgcctct tgtaaacgtg gcaccaattg atcataaatt
    23881 ttttcatgaa ctaataatcg agaacctgca ctacatactt caccttggtt gaataaaata
    23941 cctaactgaa taccttcaac tgcaaggtct aaattagcat catctaatat gatattggcg
    24001 cttttaccac caagctctaa tgtagcgggt actagatgtt ttgctgcagc ttcggcaact
    24061 tgataaccta catcagttga gcccgtaaat gataatttat ctacaccatc atgattgaaa
    24121 attgcattac ctgattctga acctttaccc gttagtatat tgacaacacc tttaggtaat
    24181 acctcttgga aaattttagc aacttccaat aaacttaatg gtgttgaaga cgaaggttga
    24241 atcacaattg tattacctgc agcaatagct ggcgcaatct tccatgcagc taatagcatt
    24301 gggaagttcc aagcaacaac agcacctacg acgccaatcg gctcatgtcg tacgatactc
    24361 attgtgtctt tatcgatatc attcactgta ccttcttctg tttcaataac acttgcgaaa
    24421 taatggaaat gtcttgcagc aaatggaata tcaattgctg ttgtctcacg aatcggttta
    24481 ccattattta atgtttcaat cattgcaatt ttatcttttt gtgccattaa tttatcacca
    24541 atatcacgca acatttgtgc acgttctgat ttagaagtta atgaccatga ttcaaatgcc
    24601 tcttgcgcca cttcgactgc atgatcgaca tctttatctt ttgctcttgt aatatgtgat
    24661 agtgtttctc cagttgctgg attggtcact tcgattgttt cgtcactgct acctttaaca
    24721 aattccccat tgataaataa accataattc tctgcaatat aatctcgaac gtttactgcc
    24781 atattatctc tcctttgttt attggctata atataaatct cttagcatca tatttaataa
    24841 tgcttttaaa cgatatgatt cacattatat agtaagcgtt tacattttat aaaataatca
    24901 gctcacagaa gtctcatttt taaaactttt gcgtcgtttt tgcacataaa aataatctta
    24961 ttttcgaaaa catattgcac ttttcaaaaa taaaacacat aatataacta taatacttga
    25021 gaataattct cattaaacag tttggttgtt atgaaagtcg cgcttaaaat gcgttaatgg
    25081 gacaaggata attcaacaac gtttcatttt actgagtcat tgcacttatc atacacatta
    25141 tatttagcat gatttatatt actaaaacaa tattacatgc gcattagtac tagaatggag
    25201 ttttttcaat gcgacttatt ttaattgtca ttggcttaat atttacagcg cttggtattg
    25261 caggtgccgt attaccttta ctgccaacga cccctttttt actcgtagca gttttttgtt
    25321 ttgctcgaag ttcagatcgc ttttacaatt ggcttattaa tcaaaaaatt tataaagaat
    25381 atgtagaaaa cttttattta catcgaggct acacgctaca acagaaaatt aaaattttaa
    25441 ttagcttata cattgtaata ggtttttcaa tttatatggt ggatgttctt gcagtccgta
    25501 taggattaat cataatggtt atcatacaaa ccgctgtact ctttacattt gtaaaaacat
    25561 tacccaaatc aaatcataaa atagaggagt gattgcccat gtttatggca gaaaatagat
    25621 tacaattaca aaaaggcagt gcggaagaaa cgattgaacg tttttacaat agacaaggta
    25681 ttgaaactat tgaaggcttc caacaaatgt ttgtcactaa aacattaaat accaaggata
    25741 cagatgaagt taaaatctta actatttggg aatctgaaga tagctttaat aattggttga
    25801 attccgatgt atttaatgaa gctcataaaa atgtacgttt aaaaagtgat cacgacggac
    25861 agcaaagtcc aatattatca aataaagtat tcaaatatga tattggctac cactatcaaa
    25921 aataaacata ttaaaaaccc ctagtcaccg ctggttgtct aaaccaactt catgattagg
    25981 ggttttttat tatttgtgac gtaaaggtac gaattcatcc ggcttgtctg aacgcaatcc
    26041 aaaataatat tctattgctt cacaaatacg tcgtgatgct tgcccatcac catatggatt
    26101 attcgcttga ctcatacgtt gatacgcttc atcatcctca atcaattgtt tcacatttcg
    26161 aacaatattg tcataatctg tgccaattac tctcgatgtt cccgcctcaa cgccttcggg
    26221 acgctctgta tgattcctta ataccaacac aggttttcca aatgtgggag cctcctcttg
    26281 aataccacca gaatctgtca gcacgaggta cgattgattt gtaaaattat gaaactcaat
    26341 cgcatctaat ggctcaatta attcaatccg atttctccca gataaatatt tttcggcaat
    26401 cgctcttacc tttggattac gatgcattgg ataaatgaag acaacatctt tatattcatc
    26461 tgccaaatct cttactgctt taaaaatctg atgcatcggt tccccaatat tttcacgacg
    26521 atgcgctgtt agtaaaataa ctttcttgcc tttatgttta ttaataatcg ttgaaacaaa
    26581 atcattttga actgttgttg acaatgcgtc aataactgta tttccagtaa taaagatacg
    26641 ctctttgtct ttgttttcaa aaagtaaatt tttagctgca attactgttg gcgcaaaatt
    26701 caattcagca atattactta ccatgactcg atttaactct tcaggaaatg gtgagtattt
    26761 ctgatgtgtt cgaagtccag cttctacatg tccgaccgga atttgatgat aaaatgctgc
    26821 caaacttcct acaaaagtcg ttgtagtatc accatgtact aaaatcatat ctggctgttc
    26881 ctcattaatg atgctatcaa gtttagcaag tgcattcgcc gtaatgcccg ctaacgtttg
    26941 ttgatcttgc ataatattta aatcatgatc agcttgaata tcaaatatac ttaacacact
    27001 atctaacata tctctatgtt gtgctgtaat cacaatgttc gcttcaaagt tcccattatg
    27061 atcaatttct tttactaatg gtgccatttt tattgcttcg ggtctcgtac cgaaaataac
    27121 cataattttt ttcataacat tatcctctct gaagttcaaa cacatgacag cttatctttc
    27181 cttaatcatg tatgccctag tttgacacat tttatttgtc gataaaatta aatatattgc
    27241 cataattata atacgataca tcttcaaatg atgatttcac aacatttttt gtatcaaaaa
    27301 tcactttatg cttcatttta tcaaaatgac tgtccgataa atttttaaat tctgagtggt
    27361 cacttaaaat caatactagc gatgcgtctt tgacagcatg tgacatatca tgatccacaa
    27421 aatctaattc aacatgtgga tcataagcac atacttctat gtctggttct tgatttaata
    27481 gctcataaat atcaaatgct ggtgattctc ttatatcatc aacatcacct ttataagtta
    27541 aaccaaatac tgtgacttta ttcccgctca acgctttgat gatttgcttc gttgtatcaa
    27601 caacataggc cggcattgaa ttattaattt cacgtccagt ttgaattaac tttgcatttt
    27661 cagggtcttt agcaataata aagtacggat caacagctaa acaatgaccg cctacacctg
    27721 gaccaggttg atggatatta acacgcggat gtttgtttgc catttcaatc acatctaata
    27781 cattaatatt taagttattg caaatttttg ttaattcatt agctaaagca atattcacgt
    27841 ctctatatgt gttttccatt agcttactca tttcagcagt acgtgcatct gtttcaatca
    27901 tttctccctg aacgaatgtg cgatagacac gtttacccgc ttcaatacaa gctttagtca
    27961 cgccgccaat gatgcgattg ttatgaacta attcttctaa aatttttcct ggcagtacac
    28021 gttctggaca atgcactaaa taaatatctt cacctattgt aaaccctaaa ttttcaatga
    28081 ctggttttac aaaatcatcc atcgttttag gcgcaattgt cgactctaca ataatggtat
    28141 ttcctttttc taaaaatggt aaaatactat ctaatgcacg cataactagc gaaatgtcac
    28201 atgaccggta ctgatcatca ttattcggcg tcggaacggc aacgataaaa acatcagatg
    28261 cttctggcgt tgtagatacc ttcaattttc ccgatgacag tacctcttca taaacctttt
    28321 gtaatccagg ttcttcaata ctaatttgac cactttgcaa cttatcaatc gtttgctgat
    28381 taatatcaac accaagcaca tcgacgccat gctttgcaaa cataattgat gttggtaaac
    28441 caatataacc taagccaact actgttaact tcatactata acctccaaat aaaacgattt
    28501 gtatgttgat tttatttaat agatgactta tgccttatct ttggttgtcg tttcatccat
    28561 aatgtccgca ataacaagtg acatttttcc aggaataact tcaagtgcat tattattttc
    28621 atataacgta ttgctgtatg ttaaattgcc gaataatttt ctaaagaccg acaacttatt
    28681 aaaatactta tttagcattg aagacatgtt gatcaatacc gttttacgat gtgattggcg
    28741 acgtatttca tacattactg acgatgtatc aaagtaaaaa ctatcttgag gatgatacac
    28801 gcctgtcact tctaatgata ttaattgatc aacaaatgct gtcagatgtt taatatataa
    28861 tgcactgcgc tgattgttaa tatttggaat gattggcaat cgctttgaca attgcattaa
    28921 ccgttggaaa tttcctgggc aatgtgcacc ataaatcatt ggtggtctca caattgctac
    28981 tttaaacgaa tcactaatca actcttgtaa tgcttgttca gcgaactttt tggaaatacc
    29041 ataattggtc gtagggttca ttgatgtttg tgtatcaatt tgatctgatt taccaacctg
    29101 accttctttt ccataaactg ccatagtact cataaaaata aattgtttaa cgtcttcagc
    29161 tttagccttt tgtgccaatt gtttcgtaag caacatattc acttgcatat aatcagatag
    29221 ccttgcttga ggtgaattgt tgtgaaccaa agctgctgta tgaattaaaa catcataacc
    29281 tttgaacgag gtcgacttcc ataattgatt cctaacatta atttgatcca cttgatgtcc
    29341 ttgttcaata agcttatctt ttaaagcatt accgatatat ccatgtacgc ctgtaattaa
    29401 aatatttttt ctcattagtg atgcacacct tctgaagtaa cgatattttt aattgtttta
    29461 tatatgatat acatatcaag catcatagat tgatgtgtta agtaataatg atcatacgct
    29521 actttttgat catcagtgat atcatctctc cccatcactt gagctagtcc tgtcacacct
    29581 ggtctaatcg tatgcacgtt cgcttttgta cgtttttcga ttaattcgta ttgattataa
    29641 agcgctggtc taggacctac aattgacatt tctcctttta aaacattcaa taattgtggc
    29701 aattcatcaa tagaggtctt acgaatgacc ttccctgtct ttgttatata cgatgttgaa
    29761 tccattaaat cagttgcaac attaggtgtg tctattttca ttgatctaaa cttataaata
    29821 ttaaacaatt cattattaat cgtcggtctt ttttgtttga aaatggctgg tccaggtgat
    29881 tccattttaa ttagtaatgc tgtaattaac agaatcggac ttaaaactac taaaccatat
    29941 attgaactca ctacatcgaa taatcgtttc ataattatct cctcacttca acagattgta
    30001 atacttcata gtattaattt ctctcgtata tttatcttta aacagttgat gtgcgcgctc
    30061 acccatctcg tgcaattcct tactcgagtg agtgttgatg aagttataaa tggcatgtgc
    30121 atcgccatta tcaatttgga tacccgcatc atattgttca acatgttgaa cgatatcaga
    30181 ttgcttatcc atgatgagta ccaacggctt cttagctgca aggtagccat aattcttgct
    30241 cggcacgcct aaaccaacgc cttctttaat cagcgatgca atacatacat ccgcaatttt
    30301 taatacgtca gcatagtctg tacctgttaa aaactcaaac attttaacat tttcaatacg
    30361 atggtcttct attgccgttt tgacatctgc aaatttctta ccatgaccac aaagtattgt
    30421 taacgtttga ggctgatcct tatttaattt taaaaatgag ataagtgtct ccatatcctg
    30481 taactgcccc atattaccgc tatacaataa aattttgtcg tattgctcac ggtaagcttt
    30541 aaatgtgtca ttatagatac gattgtcttg taactgacgc atgtcatacc agttaggaat
    30601 cacatgaata ttgtcagcat ttttagaaat ttgatgattt agtaaatagt ttttcatttc
    30661 cgtaccaagg acaatgacat tttcagcatt cttgtagaca tgtctattaa tgtaacgcat
    30721 cagtttatca atcatgctac ctggacgagt tgaacctgtc ttaatcgcat tatcaggtgc
    30781 tatatcatac accacaaaag aatatttttt cttaagcagt ctgtgtaaaa cgtctggtat
    30841 taatggcaag attggtggat tagagtaaac aagaatctga tcatatttca acattttagg
    30901 tatattaatt acgaattttg aaaataaact aaagaaattg atgatccttc caaccttact
    30961 tttgttatta aacctcgaat acttgagacg tcgaatgcga ataccacgat gcatctcggt
    31021 tttagaaacc tgtttatgat tactatattc atatggccat ccacacatga catcgacatt
    31081 aatgtgattc gcaattaaat cttccgccaa ttgtgttggt aacgtcgctg aagatacata
    31141 ttccggataa aaatactgac ataaaatcaa aatctttttt tcactcatct aatcgacgtc
    31201 ctttttatta atgaatttag actaagaatt tttgaactgt ttatttttag caaaataata
    31261 gagtgctatg aaaaatatca tgtacgatac gattaaaaca attgtattaa tagatacaaa
    31321 gtttaaaaaa gttaaattaa gcatttttga tataacaaat gttgcagtag tacctattaa
    31381 aaataacgct tgcagtatta attctaactg ttgtttttgt aatacaatca cagaaagtga
    31441 taccgttgat acaatcagtc ttataacaaa taatgggata acaattttta taactgtaat
    31501 tgcatcaacc cagctgtgcc ctaataatat attgactaat tcttcactga acaaataaag
    31561 tggcacataa agaattatag aaactataac cagtatgaaa ctaaatttaa gtaataaatt
    31621 tcggaagtta ccatgttcaa tataatagtc attagcttgt tgcataaata tttttgataa
    31681 ccctaatgaa ataattgtca ctggtatgcc tagtactctt atggccatac cataaatacc
    31741 tacttctgta ttggtataca aaatacctat aaagaacaca acaaccgaga aagacaaact
    31801 atttaataat attgatggtg ttgaataaac taactgattt ttattttcta aaaataatgc
    31861 tttagtttct tctttatctc tcacaatatt aaagtgcttt ttaaacgttt tatatcctag
    31921 tgtaatgcct gcgatatagg ataatgaaaa accaataatt aatcctagac ttcctaatgc
    31981 taaaagtccg aaaatgattt gaagtaaagc cattgatcca gctctaaaca cattaatcaa
    32041 acttaacact ttatattttc tttccttatt tagataattt gtaaaaatgt ttgttaaact
    32101 taccaaaaac aacataataa taatgaaaat aaaatctaat ataaagtttg ggtataacca
    32161 tgcactaatc gcaaatattg gtatcagtat taataaaata agcaatgata tcaaaaaact
    32221 gatttgtgaa agtatattag cactacggtc atttttggta ttcacaatca acaaatcgta
    32281 tcttgcattt attattggta ttaaaatcat tgcgatattt gaaaaaattg taaactcacc
    32341 aaattctgta ggtgaatata gtcttgtaat aattggggta gtgatgatta atatgacttg
    32401 agcgatgcca ctgcttaaaa tcattaagaa cgaatcgcca ataaatttat ttaatctcat
    32461 aattaacctc agattttctt gatgactttc gctggattgc cacctataac aacgttacct
    32521 tctggaaaac tttttgttac aaccgatcca gcccctacaa ttgtatgttc ccccaattct
    32581 actcctggca atataactga gttcattcca atccaactat aattccctat tttgacatct
    32641 tcacctggtg catgtgactt caaattttta atattatgat tagctgtaat aatacctacg
    32701 ttaggcgcta tataaacacc tctacctata tatataactg ctgaaaaatt attaaaatac
    32761 gtcccaggcg attggaaaat atgaatatca ttcttatcaa aaacaatgtt attagggtta
    32821 tgcattctaa cagttatatc tgcaggaaat ggcaagctcg tatttattcc taacaacttg
    32881 aacggaacag ctttccacgc ccataaccat ccagccactt tattttcaaa ataatatcca
    32941 gttaaatatt ctttcttata aaataataat agtggtgtat tcattaatct aaatactttc
    33001 gatttcataa atgtttttac aatcattcta cccctacttt tttagtgaat ttttttatta
    33061 taatgcctgg gataatagct ataacatata caactaaaat catagaaatc aatgtgatta
    33121 atggtgtcgg gtgactaaaa atactcgtaa taagtctcat ataaatatat gtaccaataa
    33181 atatcacttc taacgaatga ctcttccgaa taatatactc actcagaaat gcaaaaaaga
    33241 tatttgcaat taaattcaaa tagaagaata agggtccaaa ataaataatt ccatatccag
    33301 cactcgaaat tagatgacca gtcaattgtt tactaccata tatcaattgg ttaaaaagtt
    33361 ggctagtaat caactgcttt ttatctacaa agaaattaaa cccaaaaaca gctctagtat
    33421 tatcaaataa atattgtttg aacgaaccat tatagtaatt caaatagtct atagaagctg
    33481 caatattgct aggtccataa aaatatgact gcaacgtgtc aactatctta agattactta
    33541 cacttgtact atttaacgcc tcgctatatg aactataatt aaacacatac aattctttat
    33601 atagtgtcat caatgctaat acgaaaatac caacagatat aataatgata ttgatacgtc
    33661 ttttgtgctt agaaaacaag agtgacacca ctgttaaaac agcaaccatc gtataaagtt
    33721 gataacttct tctttcacca acaattaaac taatatttaa aattcctata gctagcggca
    33781 atacaacata aataatttta ggattctctt tatacttttt atatgaagca tatgcgatta
    33841 ttaagaataa taacgctaag gcaagttgaa atagcattac aaaaaggaca tttaaaccac
    33901 ttgttacttc ggttcctctt cccattgcat ctgttttaat aattaaaaat gatatgtttt
    33961 tgcgcacttc gggaaaagct acaaaaatcc cacaaataac aagaccaaac acaatgtaat
    34021 aaattgatga tccagaaagt gtaaagctat ttcttgatac cacttcatgt ttgaataatt
    34081 tagatagtat caatataaat agtgagccta tgataaactc cacactcata taaataaccg
    34141 cttgattaaa ataagcttgt cctaatggta aattatctat agttgaatcc tggaaaaaca
    34201 ctatcataac aggtataaat actaatctaa gaaaacctaa aacatcgtat acaattttcg
    34261 aaaatttaaa attcggatta ttcgagaagc ttataaagag ctttcctact ccataactta
    34321 taggtgctaa aaataataaa ggtttatcac ctaacataag gcaaggaaac attaaaatgg
    34381 caataacata tgaagtgaca ttatatatct tattcattaa atatcctgcc tatctcttga
    34441 tagaactgtt tattattttc aattacatcg tttctataag cttctctttt atgattaatg
    34501 tccattacat ccaaattgtt ataccaatcc atcaattcat caccaatacc ttttaaattt
    34561 tctggattaa cactaaaacc cagtccgaat ttattagctt ctgtagcggt gaatgtatcg
    34621 tctgttgtaa taataggttt gtttaaaaac aacgcataat acattcttat ggataaagca
    34681 gtatctaaag caatattttg attaccaaac aaattattaa gtatatcaat tttatttaaa
    34741 tatttcgggg tttctttcaa gtcaaaaccg ccagaaaatg taatattatt aataaaattt
    34801 tctcgagcaa agacttctaa tttttccgac cctgttccaa aatattgcat atggaacctt
    34861 gaatcatttg ccagttcctt tatcaatttt tgatttatct cattaaatct aacattacca
    34921 ataaaaccta tattaattgg ccatcttttt ttgaatccat cggtca
//
LOCUS       DAHHRR010000015        34207 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.015, whole genome shotgun sequence.
ACCESSION   DAHHRR010000015 DAHHRR010000000
VERSION     DAHHRR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34207
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.015"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            98..343
                     /gene="yaaA"
                     /locus_tag="NBI42_000609"
     CDS             98..343
                     /gene="yaaA"
                     /locus_tag="NBI42_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCD1777054.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GARETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            340..1452
                     /gene="recF"
                     /locus_tag="NBI42_000610"
     CDS             340..1452
                     /gene="recF"
                     /locus_tag="NBI42_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCD1777055.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            1462..3396
                     /gene="gyrB"
                     /locus_tag="NBI42_000611"
     CDS             1462..3396
                     /gene="gyrB"
                     /locus_tag="NBI42_000611"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCD1777056.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNEKKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            3433..6093
                     /gene="gyrA"
                     /locus_tag="NBI42_000612"
     CDS             3433..6093
                     /gene="gyrA"
                     /locus_tag="NBI42_000612"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCD1777057.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDSSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDADEVNEDEQSTVSEDGTEQQREAVVNDETPGNAIHTEVIDSEE
                     NDEDGRIEVRQDFMDRVEEDIQQSSDEDEE"
     gene            complement(6175..6987)
                     /locus_tag="NBI42_000613"
     CDS             complement(6175..6987)
                     /locus_tag="NBI42_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCD1777058.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            7313..8827
                     /gene="hutH"
                     /locus_tag="NBI42_000614"
     CDS             7313..8827
                     /gene="hutH"
                     /locus_tag="NBI42_000614"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HCD1777059.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQTNIEIVDEALERVKKSRDVVER
                     IIENEETVYGITTGFGLFSDVRIDSTQYNELQVNLIRSHACGLGKPFSKEVALVMMVL
                     RLNTLLKGHSGATLDLVKQLQFFINERIIPVIPQQGSLGASGDLAPLSHLALALIGEG
                     KVFYKDVEMDSADVLKQLNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHEVHAVRNFQEQINVAARMRDWLEGSTLTTRQAEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEADETFVISGGNFHGQPI
                     TFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYEEFRNIVPSITHDRQFHKDIEAVAHYLKQSIYQTSECH"
     misc_binding    8900..9115
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9205..10491
                     /gene="serS"
                     /locus_tag="NBI42_000615"
     CDS             9205..10491
                     /gene="serS"
                     /locus_tag="NBI42_000615"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCD1777060.1"
                     /translation="MLDIRLFRNEPDTVKCKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDNQLNEIDNKMTSILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFDFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKSIK"
     gene            11138..11833
                     /locus_tag="NBI42_000616"
     CDS             11138..11833
                     /locus_tag="NBI42_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCD1777061.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIIL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFEPITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMER"
     gene            11830..12159
                     /locus_tag="NBI42_000617"
     CDS             11830..12159
                     /locus_tag="NBI42_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCD1777062.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AVLRFFF"
     regulatory      12364..12462
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            12522..13490
                     /locus_tag="NBI42_000618"
     CDS             12522..13490
                     /locus_tag="NBI42_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCD1777063.1"
                     /translation="MTNYTVDTLNLGKFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDTHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYMTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALSERFKYHRHFFVPDNVGHDGFLLNFNTWAPNLYHFLNLKHFKRK"
     gene            13783..14721
                     /locus_tag="NBI42_000619"
     CDS             13783..14721
                     /locus_tag="NBI42_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCD1777064.1"
                     /translation="MGVAYVFSKIQPKATMIATITLVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LFIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            14736..16703
                     /gene="gdpP"
                     /locus_tag="NBI42_000620"
     CDS             14736..16703
                     /gene="gdpP"
                     /locus_tag="NBI42_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HCD1777065.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSEDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            16700..17146
                     /gene="rplI"
                     /locus_tag="NBI42_000621"
     CDS             16700..17146
                     /gene="rplI"
                     /locus_tag="NBI42_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCD1777066.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            17178..18578
                     /gene="dnaB"
                     /locus_tag="NBI42_000622"
     CDS             17178..18578
                     /gene="dnaB"
                     /locus_tag="NBI42_000622"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCD1777067.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDSGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            18857..20140
                     /locus_tag="NBI42_000623"
     CDS             18857..20140
                     /locus_tag="NBI42_000623"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCD1777068.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDEIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTSVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            20570..20644
                     /locus_tag="NBI42_000624"
     tRNA            20570..20644
                     /locus_tag="NBI42_000624"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20603..20605,aa:Glu,seq:ttc)
     gene            20652..20724
                     /locus_tag="NBI42_000625"
     tRNA            20652..20724
                     /locus_tag="NBI42_000625"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20685..20687,aa:Asp,seq:gtc)
     gene            21330..22031
                     /locus_tag="NBI42_000626"
     CDS             21330..22031
                     /locus_tag="NBI42_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1777069.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
     gene            22044..23870
                     /gene="walK"
                     /locus_tag="NBI42_000627"
     CDS             22044..23870
                     /gene="walK"
                     /locus_tag="NBI42_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCD1777070.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            23863..25197
                     /locus_tag="NBI42_000628"
     CDS             23863..25197
                     /locus_tag="NBI42_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777071.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLSSDFTLFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            25198..25986
                     /locus_tag="NBI42_000629"
     CDS             25198..25986
                     /locus_tag="NBI42_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCD1777072.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPASILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTAADGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGSEYQLSEISSGSVKYEQTYDNFPILNNSKAMLNFNI
                     EDNKAASYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            26375..27175
                     /locus_tag="NBI42_000630"
     CDS             26375..27175
                     /locus_tag="NBI42_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1777073.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPVDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAGHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            27402..29720
                     /gene="adsA"
                     /locus_tag="NBI42_000631"
     CDS             27402..29720
                     /gene="adsA"
                     /locus_tag="NBI42_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HCD1777074.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWHVSNAAEQHTPMKAQAVTTIDK
                     ATTDKQQVTPTKEAAHHYGEEAATNVSASAQGTADEINNKVTFNAPSNKPSTAVSTTV
                     NETHDVDAQQASTQKPTQSATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSESGKDTQPIDKDKVMNPA
                     KQPASGKVVLLPAYRGTVSSGREGSDRALEGTAVSSKSGKQLASMSAPKGSAHEKQLP
                     KTGTDQSSSPAAMFVLVTGIGLIATVRRRKAS"
     gene            30088..30567
                     /gene="rlmH"
                     /locus_tag="NBI42_000632"
     CDS             30088..30567
                     /gene="rlmH"
                     /locus_tag="NBI42_000632"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCD1777075.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNHRMTQGQSD
                     FVFVIGGSNGLHKDILQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            30889..32145
                     /locus_tag="NBI42_000633"
     CDS             30889..32145
                     /locus_tag="NBI42_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HCD1777076.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMSLNEMFAMKEYKQKYLILRIFNVSGKEPQFYFIDPYANYS
                     EFKDVDDLIDKVFNVEAIQYKVFGEK"
     gene            32560..32799
                     /locus_tag="NBI42_000634"
     CDS             32560..32799
                     /locus_tag="NBI42_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777077.1"
                     /translation="MSDNLSLFIDYINDNIIYGSEIKREKLENLFNQFAIKNVEKNIV
                     YDELKSLDITIIESQDSYKNKLKRLFSVLLQSKKI"
     gene            complement(32831..33505)
                     /locus_tag="NBI42_000635"
     CDS             complement(32831..33505)
                     /locus_tag="NBI42_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HCD1777078.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDI
                     WLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSK
                     YLNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEI
                     SIMLAS"
     gene            33559..33930
                     /locus_tag="NBI42_000636"
                     /pseudo
     CDS             33559..33930
                     /locus_tag="NBI42_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
BASE COUNT        12177 a         5117 c         6654 g        10259 t
ORIGIN      
        1 aattaattat ttatatgtaa acggtttcta cctctatttt aaatgaaatt tgtgacaaaa
       61 aaaggtataa tatattaatg acatacaaag aaatggagtg attattttgg ttcaagaagt
      121 tgtagtagaa ggagacatta atttaggtca atttctaaaa acagaaggga ttattgaatc
      181 tggtggtcaa gcaaaatggt tcttgcaaga cgttgaagta ttaattaatg gagcgcgtga
      241 aacacgtcgc ggtaaaaagt tagaacatca agatcgtata gatatcccag aattacctga
      301 agatgctggt tctttcttaa tcattcatca aggtgaacaa tgaagttaaa tacactccaa
      361 ttagaaaatt atcgtaacta tgatgaggtt acgttgaaat gtcatcctga cgtgaatatc
      421 ctcattggag aaaatgcaca aggaaagaca aatttacttg aatcaattta taccttagct
      481 ttagcaaaaa gtcatagaac gagtaatgat aaggaactca tacgttttaa tgctgattat
      541 gctaaaatag aaggtgagct tagttataga cacggcacga tgccattaac aatgtttata
      601 actaaaaaag gtaaacaagt caaagtgaat cacttagagc aaagtcgtct aactcaatat
      661 attggacacc tcaatgtggt tctatttgcg ccagaagatt tgaatattgt aaaaggctct
      721 cctcaaataa gacgacgctt tatagatatg gagttgggcc aaatttctgc tgtttactta
      781 aatgatttag ctcaatacca acgtatttta aagcaaaaga ataattactt aaagcagtta
      841 caattaggcc aaaaaaagga cttaacaatg ttggaagtat taaatcagca gtttgctgaa
      901 tatgcaatga aagtaactga taaacgtgca cattttattc aagagctaga gtcattagct
      961 aaaccgattc atgctggtat cacaaatgat aaagaagcgt tgtcgttgaa ttatttacct
     1021 agtcttaaat ttgactatgc tcaaaatgaa gcggcacgac ttgaagaaat tatgtctatt
     1081 cttagcgata atatgcaaag agaaaaagaa cgaggcatta gcttattcgg accacatcga
     1141 gatgatataa gttttgatgt gaatggcatg gatgctcaaa catatggttc tcaaggacag
     1201 caacgtacaa cggctttgtc cattaaatta gctgaaattg agttaatgaa tatcgaagtt
     1261 ggggaatatc ccatcttatt attagacgat gtactcagtg aattagatga ttcgcgtcaa
     1321 acgcatttat taagtacgat tcagcataaa gtacaaacat ttgtcactac gacatctgta
     1381 gatggtattg atcatgaaat catgaataac gctaaattgt atcgtattaa tcaaggtgaa
     1441 attataaagt aacagaaagc gatggtgact gcattgtcag atgtaaacaa cacggataat
     1501 tatggtgctg ggcaaataca agtattagaa ggtttagaag cagtacgtaa aagaccaggt
     1561 atgtatatag gatcgacttc agagagaggt ttgcaccatt tagtgtggga aattgtcgat
     1621 aatagtatcg atgaagcatt agctggttat gcaaatcaaa ttgaagttgt tattgaaaaa
     1681 gataactgga ttaaagtaac ggataacgga cgtggtatcc cagttgatat tcaagaaaaa
     1741 atgggacgtc cagctgtcga agttatttta actgttttac atgctggtgg taaattcggc
     1801 ggtggcggat acaaagtatc tggtggttta catggtgttg gttcatcagt tgtaaacgca
     1861 ttgtcacaag acttagaagt atatgtacac agaaatgaga ctatatatca tcaagcatat
     1921 aaaaaaggtg tacctcaatt tgatttaaaa gaagttggca caactgataa gacaggtact
     1981 gtcattcgtt ttaaagcaga tggagaaatc ttcacagaga caactgtata caactatgaa
     2041 acattacagc aacgtattag agagcttgct ttcttaaaca aaggaattca aatcacatta
     2101 agagatgaac gtgatgaaga aaacgttaga gaagactcct atcactatga gggcggcatt
     2161 aaatcttatg ttgagttatt gaacgaaaag aaagaaccaa ttcatgacga accaatttat
     2221 atacatcaat ctaaagatga tattgaagta gaaattgcga ttcaatataa ctcaggatat
     2281 gccacaaatc tgttaactta cgcaaataat attcatacgt atgaaggtgg tacgcatgaa
     2341 gatggattta agcgtgcatt aacgcgtgtc ttaaatagtt atggtttaag tagcaagatt
     2401 atgaaagaag aaaaagatag actttctggt gaagatacac gtgaaggtat gacagcaatt
     2461 atatctatca aacatggtga tcctcaattc gaaggtcaaa cgaagacaaa attaggtaat
     2521 tctgaagtgc gtcaagttgt agataaatta ttctcagagc actttgaacg atttttatat
     2581 gaaaatccac aagtcgcacg tacagtggtt gaaaaaggta ttatggcggc acgtgcacgt
     2641 gttgctgcga aaaaagcgcg tgaagtaaca cgtcgtaaat cagcgttaga tgtagcaagt
     2701 cttccaggta aattagccga ttgctctagt aaaagtcctg aagaatgtga gatattctta
     2761 gtcgaagggg actctgccgg ggggtctaca aaatctggtc gtgactctag aacgcaggcg
     2821 attttaccat tacgaggtaa gatattaaat gttgaaaaag cacgattaga tagaatattg
     2881 aataacaatg aaattcgtca aatgatcaca gcatttggta caggaatcgg tggcgacttt
     2941 gatctagcga aagcaagata tcacaaaatc gtcattatga ctgatgccga tgtggatgga
     3001 gcgcatatta gaacattgtt attaacattc ttctatcgat ttatgagacc gttaattgaa
     3061 gcaggctatg tgtatattgc acagccaccg ttgtataaac tgacacaagg taaacaaaag
     3121 tattatgtat acaatgatag ggaacttgat aaacttaaat ctgaattgaa tccaacacca
     3181 aaatggtcta ttgcacgata caaaggtctt ggagaaatga atgcagatca attatgggaa
     3241 acaacaatga accctgagca ccgcgctctt ttacaagtaa aacttgaaga tgcgattgaa
     3301 gcggaccaaa catttgaaat gttaatgggt gacgttgtag aaaaccgtag acaatttata
     3361 gaagataatg cagtttatgc aaacttagac ttctaagcgc tgtgaactga acttttgaag
     3421 gaggaactct tgatggctga attacctcaa tcaagaataa atgaacgaaa tattaccagt
     3481 gaaatgcgtg aatcattttt agattatgcg atgagtgtta tcgttgctcg tgcattgcca
     3541 gatgttcgtg acggtttaaa accagtacat cgtcgtatac tatatggatt aaatgaacaa
     3601 ggtatgacac cggataaatc atataaaaaa tcagcacgta tcgttggtga cgtaatgggt
     3661 aaatatcacc ctcatggtga ctcatctatt tatgaagcaa tggtacgtat ggctcaagat
     3721 ttcagttatc gttatccgct tgttgatggc caaggtaact ttggttcaat ggatggagat
     3781 ggcgcagcag caatgcgtta tactgaagcg cgtatgacta aaatcacact tgaactgtta
     3841 cgtgatatta ataaagatac aatagatttt atcgataact atgatggtaa tgaaagagag
     3901 ccgtcagtct tacctgctcg attccctaac ttattagcca atggtgcatc aggtattgcg
     3961 gtaggtatgg caacgaatat tccaccacat aacttaacag aattaatcaa tggtgtactt
     4021 agcttaagta agaaccctga tatttcaatt gctgagttaa tggaagatat tgaaggtcct
     4081 gatttcccaa ctgctggact tattttaggt aagagtggta ttagacgtgc atatgaaaca
     4141 ggtcgtggtt caattcaaat gcgttctcgt gcagttattg aagaacgtgg aggcggacgt
     4201 caacgtattg ttgtcactga aattcctttc caagtgaata aggctcgtat gattgaaaaa
     4261 attgcagagc tcgttcgtga caagaaaatt gacggtatta ctgatttacg tgatgaaaca
     4321 agtttacgta ctggtgtgcg tgtcgttatt gatgtgcgta aggatgcaaa tgctagtgtc
     4381 attttaaata acttatacaa acaaacacct cttcaaacat catttggtgt gaatatgatt
     4441 gcacttgtaa acggtagacc gaagcttatt aatttaaaag aagcgttggt acattattta
     4501 gagcatcaaa agacagttgt tagaagacgt acgcaatata acttacgtaa agctaaagat
     4561 cgtgcccaca ttttagaagg attacgtatc gcacttgacc atatcgatga aattatttca
     4621 acgattcgcg agtcagatac agataaagtt gcaatggaaa gcttgcaaca acgcttcaaa
     4681 ctttctgaaa aacaagctca agctatttta gacatgcgtt taagacgtct aacaggttta
     4741 gagagagaca aaattgaagc tgaatataat gagttattaa attatattag tgaattagaa
     4801 gccatcttag ctgatgaaga agtgttatta cagttagtta gagatgaatt gactgaaatt
     4861 agagatcgtt tcggtgatga tcgtcgtaca gaaattcaat taggtggatt tgaagactta
     4921 gaggacgaag acttaattcc agaagaacaa atagtaatta ctttgagcca taataactac
     4981 attaaacgtt tgccggtatc tacatatcgt gctcaaaacc gtggtggtcg tggtgttcaa
     5041 ggtatgaata cattggaaga agattttgtc agtcaattgg taactttaag tacacatgac
     5101 catgtattgt tctttactaa caaaggtcgt gtatacaaac taaaaggtta tgaagtgcct
     5161 gagttatcaa gacagtctaa aggtattcct gtagtgaatg ctattgaact tgaaaatgat
     5221 gaagtcatta gcacaatgat tgctgttaaa gaccttgaaa gtgaagacaa cttcttagtg
     5281 tttgcaacta aacgtggtgt cgttaaacgt tcagcattaa gtaacttctc aagaataaat
     5341 agaaatggta agattgcgat ttcgttcaga gaagatgatg agttaattgc agttcgttta
     5401 acaagtggtc aagaagatat cttgattggt acatcacatg catcattaat tcgattccct
     5461 gaatcaacat tacgtccttt aggccgtaca gcaacgggtg tgaaaggtat tacacttcgt
     5521 gaaggtgacg aagttgtagg gcttgatgta gctcatgcaa acagtgttga tgaagtatta
     5581 gtagttactg aaaatggtta tggtaaacgt acgccagtta atgactatcg tttatcaaat
     5641 cgtggtggta aaggtattaa aacagctacg attactgagc gtaatggtaa tgttgtatgt
     5701 atcactacag taactggtga agaagattta atgattgtta ctaatgccgg tgtcattatt
     5761 cgactagatg ttgcagatat ttctcaaaat ggtcgtgcag cacaaggtgt tcgcttaatt
     5821 cgcttaggcg atgatcaatt tgtttcaacg gttgctaaag taaaagagga tgcagacgaa
     5881 gtaaatgaag atgaacaatc tactgtatct gaagatggca ctgaacaaca acgtgaagcg
     5941 gttgtaaatg atgaaacacc aggaaatgca attcatactg aagtgattga ttcagaagaa
     6001 aatgatgaag atggacgtat tgaagtaaga caagatttca tggatcgtgt tgaagaagat
     6061 atacaacaat catcagatga agatgaagaa taaaaaaaaa taagacttcc ctatatgtag
     6121 gggagtctta tttttatgct agaaagtaat gctgtattca atgattaata ttgactaact
     6181 ttctaattgt ttcattgcat aaggtatttc attgataagt cttgatggtg gcaccacata
     6241 catatctttt gcaagatttt cgccaataaa actatgtgta tatgtggcac tcataaccgc
     6301 ttcttttaaa ttatcgaatt gaccaacaaa acttgtaatc ataccagcaa gtgtatcgcc
     6361 cataccacca gtcgccattg ctgggctacc gattgttaat ttaaagtctt catctttaaa
     6421 gaaaatttca gtaccatgtt ttttaagtac aacagttgcg cctaaacgat caactgcttc
     6481 acgattacgc tcatatgtct gttcctcaat aggaatacca cttaatcgct cccactcttt
     6541 gaggtgcgga gtgaatatta cacgacaagt aggtaattgc ggtttcagtt tactaaatat
     6601 tgtaatcgca tcgccgtcta cgattaaatt ttgatgcggt tgtatatttt gtagtaggaa
     6661 tgtaatggca ttattccctt tgaaatcaac gccaagacct ggaccaatta gtatactgtc
     6721 agtcatttca atcattttcg tcaacatttt cgtatcatta atatcgataa ccatggcttc
     6781 tggacaacga gaatgtaacg ctgagtgatt cgttggatgt gttgctacag tgattaaacc
     6841 actaccgcta aatacacatg cacgagccgc taacatgatg gcaccaccta agttagcaga
     6901 tccaccaatt aataaaattt tgccataatc acctttatgt gaatcttctt tacgcttagg
     6961 aatgttaata gaatttaacg tttccatagt gatataacct cccatgtaaa agccttttcc
     7021 gaatttattc aattttaaaa atatatagta acttttaaca aaatgtatta taaatttctg
     7081 aattcattat tctttgtcgt taaatacaat agaaaatact atacctgtat atgcaattcg
     7141 tcaatagata aattattaaa tatacttaca acaatattaa tatcctttaa cacgctacaa
     7201 tagtgctctg ataataggtt ataaatgtac ttaaaaccat tgtttcaata aaaatgaaaa
     7261 cgtatacttc aagaaggatg ggttacttaa tattaacaag ggggtaacat gtatgacatt
     7321 atatttagat ggagaaacac taacaattga ggatattaaa tcatttttac aacaacaaac
     7381 gaatatagaa attgttgatg aggcgttaga acgtgtcaag aaaagtagag atgtagttga
     7441 acgtattatt gaaaatgaag aaacagttta tggcattaca acaggattcg gattatttag
     7501 tgatgtgcgt atagattcaa cacaatacaa tgaattacaa gtgaatctta tacgttcaca
     7561 tgcgtgtgga ttaggtaagc cattttcaaa agaagtagca ctcgttatga tggtattaag
     7621 attaaataca ttattaaaag ggcattcagg tgctacctta gatctcgtaa aacaattaca
     7681 attctttata aatgagcgta ttataccagt gataccgcaa caaggttcac tcggtgcatc
     7741 aggagattta gcaccactat cacatttagc attagcatta attggtgaag gcaaagtgtt
     7801 ttataaagat gtagaaatgg atagtgcaga cgtattaaag caattaaata gacaaccttt
     7861 gaaccttcag gctaaagaag gattagcatt gattaatggt acgcaagcta tgacagcaca
     7921 aggtgtcata agttatatag aggcagaaga tttagggtat caatctgaat ggattgcagc
     7981 attaacacat cagtcgctca atgggattat agatgcatat cgtcatgaag tgcatgctgt
     8041 tcgaaatttt caggaacaaa ttaatgtagc agctcgcatg cgcgattggt tagaaggttc
     8101 aactttaacg acccgacaag cagaaattcg tgtacaagac gcgtacacac tacgttgtat
     8161 tccacaaatt catggcgcaa gttttcaagt atttaactat gttaaacagc aattagaatt
     8221 tgagatgaat gcggctaatg ataacccatt aatatttgag gaagcggatg aaacatttgt
     8281 catttctggt ggtaacttcc atggacaacc gattaccttt gctttggatc atcttaaatt
     8341 aggtgtaagt gaattagcga atgtatctga gcgtcgcctc gagagactag taaatcctca
     8401 gttaaatggc gatttaccag catttcttag tccggaacca ggattgcaaa gtggtgcaat
     8461 gattatgcaa tatgctgcag caagcctagt ttctgaaaat aaaactttag ctcaccctgc
     8521 aagtgtagat tctattacat cgtctgcgaa tcaagaagat catgtatcta tgggtaccac
     8581 agctgctaga catggttatc aaattattga aaatgcaaga cgtgtgctgg caattgagtg
     8641 tgtaattgca ttacaagcag cagaattaaa aggtgttgaa ggtttatcac caaaaacacg
     8701 tcgtaaatat gaggagtttc gtaatatcgt gccgtctatt acacatgacc gtcaatttca
     8761 taaagatatt gaagcggttg cacattattt aaagcaatct atttatcaaa cgtctgaatg
     8821 tcattaaatc gacatgaagt aagttcttaa tcgttacttg aaaaagcaaa tatagtttgc
     8881 tatattaaaa atagcttaat aagacattgt tcgtaggaca agtaatatat agtgttcgat
     8941 atcagagagc ttgtggttag tgtgaacaag aatcaacata tatatgaatc tacctactta
     9001 tttaaaagaa caatcggtga taaccgttat tttagtgaag tgcaatttag gtttagtgta
     9061 tctttataac ttaaattgtt aaatagggtg gcaacgcgta gaccacgtcc cttgtaggga
     9121 tgtggtcttt ttttattttc taaaaaataa agacgtacgt cattccataa taaatgataa
     9181 ttatttggga aaggatgaag gttaatgtta gacattagat tattcagaaa tgagcctgac
     9241 acagttaagt gcaaaattga attacgtgga gatgatccaa aagttgtaga tgaaatttta
     9301 gaattggatg agcaacgacg taaattaatt agtgcaacag aagaaatgaa agcgcgtcgt
     9361 aataaagtaa gtgaagaaat tgcattaaaa aaacgtaata aagaaaatgc cgatgatgta
     9421 atagcagaaa tgcgcacatt aggtgatgat attaaagaaa aagataatca attaaacgaa
     9481 atagataata aaatgacaag tatcctttgt cgtattccaa acttaattag tgatgatgta
     9541 cctcaaggtg agtctgacga agataacgtt gaagttaaaa aatggggcac accacgcgaa
     9601 tttgattttg aacctaaagc acattgggac attgttgaag aattaaaaat ggctgatttt
     9661 gatcgtgcag ctaaagtttc aggtgcgcgt tttgtatact taacaaatga aggtgcgcaa
     9721 ttagaacgtg ctttaatgaa ctatatgatt acaaaacata caacgcaaca tggttataca
     9781 gaaatgatgg taccacaact tgtgaatgca gatacaatgt atggtacagg tcaattacct
     9841 aaatttgaag aagacttatt taaagtagaa aaagaaggat tatatacaat tccaactgct
     9901 gaagtacctt taacaaattt ctatcgtaat gaaattattc aaccaggtgt acttcctgaa
     9961 aaattcactg gtcaatctgc atgtttcaga agtgaagctg gatcagctgg tagagataca
    10021 agaggattaa ttcgtttaca ccaattcgat aaagtagaaa tggtacgttt tgaacaacct
    10081 gaagattcat ggaatgcttt agaagaaatg acaacaaatg cagaagcgat ccttgaagaa
    10141 ttaggtttac catatcgtcg tgttatttta tgtactggtg atatcggttt tagcgcaagt
    10201 aaaacatatg atttagaagt ttggttacca agttacaatg actataaaga aattagttca
    10261 tgttctaact gtactgattt ccaagcgcgt cgtgcaaata ttcgcttcaa acgtgataaa
    10321 gcagctaaac ctgaattagc acatacttta aatggtagtg gtttagcagt tggacgtaca
    10381 tttgctgcta tcgttgaaaa ttaccaaaat gaagatggaa cagtaacaat tccagaagct
    10441 ttagtaccat ttatgggtgg taaaacacaa atttcaaaat caattaaata aagactttag
    10501 ctacaagctt taaaaagtat atatctacgt atacttaaag caagggcaag atactttaaa
    10561 taatatttta aaaagtggtg acgaagctgt cgccactttt tttgtgctgt aaaagtataa
    10621 tagtgagcca atgcacataa caacaataaa ttaagtttgt ggtttaatgg ggtgaacgca
    10681 tttcattata gcaacaatac gggataatta cgatgaacta aaacaatcta aaatgtaaca
    10741 agtttgagca tcattaatat aggaaaggaa gcgattaaat actgatttcg ttgatgtagt
    10801 atgagttata tcgatgcagt agggtagggg ggatgatgat tataagggat cgggacatga
    10861 atcaatatac cagactcatc attagatata aaaaattata aaattagtac ttaaaaacaa
    10921 ctacaaatcc gcagaaaata tagaggtagt cttaaataaa aaattgaaaa ttctcaaaaa
    10981 taaaaagtta atatgaagct atttaaagac tccggtatgt ctaacctcag acaaactgat
    11041 gtctaatgtt attgcttagg gtatagaact gtattagact aggtatatta ttttttcgta
    11101 attatataaa tataaagtgg caaaggaggt agttgagatg acaacacatt taagttttag
    11161 acaaggcgtg caagagtgta tcccaacatt attgggttat gccggtgttg gtatttcatt
    11221 tggtattgtg gcttcgtctc aaaactttag tattttagaa attatcttgt tatgtcttgt
    11281 tatatatgcc ggtgctgcgc aatttattat gtgcgcgttg tttatagcag gtacaccgat
    11341 atcagcgatt gtactaactg tatttatcgt aaattcaaga atgttccttt taagtatgtc
    11401 gcttgcacca aacttcaaga catatggatt ttggaaccgt gttggattag gttcattagt
    11461 aactgacgaa acgtttggcg tcgccattac accttattta aaaggagaag ctatcaatga
    11521 tcgctggatg catggtctta atatcacagc atatttattt tgggcaattt catgtgtagc
    11581 tggggcttta tttggcgaat atatctcaaa tccgcaaacg ttagggttag actttgctat
    11641 cacagctatg tttatctttt tggccattgc gcaatttgaa ccaattacta aatcgcgatt
    11701 aagaatttac atagtactca ttattgctgt catagtaatg atgttatcgt taagtatgtt
    11761 tatgccttca tatctagcaa tattaattgc agccacaatt tcagcagcgt taggagtgat
    11821 gatggaacga tgataactca tatgaacatg ttaatactta ttttattgtg tggtatcgta
    11881 acgctattaa ttcgaattat accttttatc atgatttcaa aagtgcaatt gcctgatgtc
    11941 gtggttcgat ggctatcatt tattccaatc acactattta cggcacttgt cattgacagc
    12001 attattcaac agacgcctca tggtgagggg tatacattaa acatccctta cattatcgcg
    12061 ctcattccga ccgttatttt atctataatc acgcgtagtt taacgattac aattattagt
    12121 gggattgtta tcatggcagt attacgattt ttcttttaaa aataactgaa aatcattgaa
    12181 cagatattta aacttaggta aactattagt aatcagaaaa ttctgatata caaatcgtct
    12241 atgttaagat tgggaatcgt gtatacgtaa gcgatttggc taacgcatga caatgataac
    12301 aacattttaa atgataaaag taattcatca ctgaatctca actaacacat aacaatttca
    12361 tatttcttat tgtgagaagt tgagggactt ggccctgtga tacttcagca accgacttta
    12421 tagcacggtg ctaaaaccaa cgagttactc gaatgataag tataaagact tcttactttt
    12481 caatagggtg agaagttttt ttgtttaagg aggaaagaac aatgacaaat tacacagtag
    12541 atactttaaa tctagggaaa tttattacag aatctgggga agtcatagat aacttgcgtt
    12601 tgagatatga gcatgtcggt tatcatggac aaccattagt tgtagtttgt catgcattaa
    12661 ctggcaatca tttaacatat ggaacagatg attatccggg ttggtggcga gaaattattg
    12721 atgggggata tatacccatt cacgattatc aatttttaac atttgatgtt attggtagtc
    12781 ctttcggttc aagctcacct ttaaatgaca ctcattttcc taaaaaatta acattaagag
    12841 atattgtcag agcgaatgaa cgaggtatac aagcccttgg ttatgacaag attaatattt
    12901 taataggggg aagtcttgga ggaatgcaag caatggaact actttacaat caacagtttg
    12961 aagtagataa agccattatc cttgctgcaa caagtcgaac atcatcttat agtagagctt
    13021 ttaatgaaat tgcaaggcaa gccattcatc ttggtggtaa ggaaggtcta agtattgcac
    13081 gccaattagg ttttttgaca tatcgatcat caaaaagtta tgatgaacgt ttcacgccgg
    13141 atgaagtagt cgcataccaa caacatcaag gtaataaatt taaagaacat tttgatttga
    13201 attgttatat gacactgcta gatgtattgg atagtcacaa cattgaccga ggtcgcacag
    13261 acgtaacgca tgtttttaaa aatttagaaa cgaaagtctt aacgatggga ttcatagatg
    13321 atttgctata tccggacgat caagttcgtg ctttaagtga acgttttaaa tatcatcgtc
    13381 atttctttgt acctgataac gttggtcatg acggattttt attaaatttt aatacatggg
    13441 cgcctaactt atatcatttt ttgaatttaa agcattttaa gcgcaagtaa taatgtatat
    13501 attttcaaag aatgaagcca ctaaatcaca gttatcatat tcaaaaacat ctagagtaca
    13561 tgagagtgga aatgaattgt aaatctaaat caagcttgat tgctatgtag tgggattaaa
    13621 taatcatgtt gaaaatgaaa gcacacaaaa gcgatttaaa tgagtatgtt aattacttat
    13681 aaaacatgaa agttattatt caattaaatg aaatagaagc atacaatcta taaattgtta
    13741 atttccatta aagaggttaa aataatagct atagttaaaa atatgggagt ggcttatgtg
    13801 ttttcaaaaa tacaacctaa agcaacaatg attgcaacga ttacgttggt atttgtcgct
    13861 ttagctttat atctagtgcc tggtttagga ctaatatttg cattatttgc aaccatacca
    13921 ggtatcgttt tatggaataa atcaatacaa tctttcggga ttagtgcact tattacagta
    13981 attataacaa ctgttttagg taatactttc gttttaagtg ccatcatatt agtcttaatt
    14041 gcaagtttaa ttattggtca attgctcaaa gaaagaacgt ctaaagaaag aatattatac
    14101 gtaacaacag tagcgatgag cttaatttca ttaatcgctt ttatgttact acaaacattc
    14161 ggaagaattc caccatcagc gagcatagta aaacctttca agcaaacatt acatgaagcg
    14221 attacgatga gcggtgccga tgcgaatatg acccaaatat tagaagaagg gtttagacaa
    14281 gcgaccgttc aattaccagg tttcatcatt atcattacat ttttaatcgt cttaattaac
    14341 ttaatcgtta catttccgat tttacgaaaa tttaaaatcg ctacacctgt atttaagcca
    14401 cttttcgcgt ggcaaatgag cggtatttta ttatggatat acattattgt tatcatatgt
    14461 ttattattta caggtcaacc gagtgtgttc cagagcattc ttttaaactt ccaacttgtg
    14521 ttatcattag tgatgtatat tcaaggttta agtgttattc atttctttgg taaagcgaaa
    14581 ggtttgccga atgcagtaac gattttacta ttgtttatcg gtacaatact gacacctacg
    14641 acacatattg taggactact tggtgttatc gatttaagtt tgaatttgaa gcgaatcatg
    14701 aaaaataatt ctaaaaagtg aatagaggtg gaataatgaa tcggcagtcc actaagaaag
    14761 ctttactaat accatttgtc atcatgatca tcacagcaat tgttttaatg ggtgtatggt
    14821 ttatctttaa tagtcttata gcattaattg catctatcgt tcttgtcgtg atgattattg
    14881 ttagcatcat tttattcaga caagctttaa tgaaaatgga tagttatgta gatggtttga
    14941 gtgctcaaat ttcaacaaca aataataaag caatcaaaca tttaccaatt ggtatcattg
    15001 ttttagatga aaatgatcac atcgaatggg ttaaccaatt tatgacagat catatggaag
    15061 caaacgtcat ttctgaatct gtaaatgaag tatttccaaa cattttaaag caattagata
    15121 gagtgaaatc cgttgaaata gaatataatc agtatcattt ccaagtacgt tattctgaga
    15181 atgatcattg cctctatttc tttgatataa ctgaacaagt acaaacaaat gaactatatg
    15241 aaaattctaa accaatcatt gcgacattat ttttagataa ctacgatgag attacgcaaa
    15301 atatgaatga tacgcagcgt tcggaaatca actcaatggt aacgcgtgtc attagtcgat
    15361 gggcaactga gtataatata tttttcaaaa gatacagttc cgatcaattc gtagcctatt
    15421 taaatcaaaa aatattagct gacttagaag aatctaaatt tgatatctta agtcaattac
    15481 gtgaaaaaag tgttggttat cgtgcccaat taacattaag tatcggtgtt ggtgaaggta
    15541 ctgaaaattt aatcgactta ggtgaattat cacaatcagg cctagactta gcattaggac
    15601 gcggtggcga ccaagttgca attaaaagta ttaatggtaa tgtgcgcttc tatggcggta
    15661 agactgaccc gatggagaaa cgtactcgtg taagagcacg tgtgatctca catgcgttaa
    15721 aagatatcct tgcagagggt gacaaagtca ttatcatggg acataaacgt cctgacttag
    15781 atgcaattgg tgcagcaatc ggtgtgtcga gatttgcaat gatgaataat ttagaagcat
    15841 acatcgtatt aaatgagact gacattgatc caacattacg acgcgtgatg aacgaaattg
    15901 ataaaaagcc agagttaaga gagcgattta ttacatcaga tgatgcttgg gatatgatga
    15961 catctaagac aaccgtagtg attgttgata cgcataaacc ggaactggtt ttagatgaaa
    16021 atgtcttaaa taaagcaaac cgtaaagttg ttatcgatca tcatagacgt ggtgaaagct
    16081 tcatctctaa tccgttgttg atatatatgg aaccatacgc aagttcaaca gcagaacttg
    16141 taacagaatt actggaatat caaccaacag aacaacgttt aacacgtctt gaatcaacag
    16201 tgatgtatgc aggtattatt gtagatacaa gaaactttac attacgaaca ggatcaagaa
    16261 cattcgatgc agcgagttat ctacgtgcac atggtgcaga tacgatttta acgcaacatt
    16321 tcttaaaaga tgatgtggat acttacatta atcgatctga attaattcga actgtaaaag
    16381 tcgaagataa tggcatagcc attgcgcatg gttcagaaga taaaatttat catccagtaa
    16441 cagttgcaca agcagcagat gaactgttaa gtttagaagg tattgaagca tcatatgttg
    16501 ttgcgagacg tgaagataat ctgattggta tatctgcgcg ttcactcggt tcagtaaatg
    16561 tccagttaac aatggaagca cttggtggcg gtggacattt aaccaatgct gcaacacaac
    16621 ttaaaggtgt gacagtcgaa gaggcgatag cacaattaca acaagcaatt acagaacaat
    16681 taagtaggag tgaagatgca tgaaagtaat ttttacacaa gatgttaaag gtaaaggtaa
    16741 aaaaggtgaa gttaaagaag taccagtagg ttatgcaaat aacttcttat tgaaaaagaa
    16801 ttatgctgta gaagcaacac caggtaacct taaacaatta gagttacaga aaaaacgtgc
    16861 aaaacaagaa cgccaacaag aaattgaaga tgctaaagca ttaaaagaaa ctttatcaaa
    16921 cattgaagtt gaagtatcag ctaaaactgg tgaaggtggt aaattgtttg gatcagtaag
    16981 tacgaaacaa attgccgaag cactaaaagc acaacatgat attaaaattg ataaacgtaa
    17041 aatggattta ccaaatggaa ttcattccct aggatatacg aatgtacctg ttaaattaga
    17101 taaagaagtt gaaggtacaa ttcgcgtaca cacagttgaa caataaagtt ggattgaaat
    17161 aagaggtgta accattcatg gatagaatgt atgagcaaaa tcaaatgcca cataacaatg
    17221 aagctgaaca gtctgtctta ggttcaatta ttatagatcc agaattgatt aatactactc
    17281 aggaagtgtt gcttcctgag tcgttttata ggggtgccca tcaacatatt ttccgtgcaa
    17341 tgatgcactt aaatgaagat aataaagaaa ttgatgttgt aacattgatg gatcaattat
    17401 cgacggaagg tacgttgaat gaagcgggtg gcccgcaata tcttgcagag ttatctacaa
    17461 atgtaccaac gacgcgaaat gttcagtatt atactgatat cgtttctaag cacgcattaa
    17521 aacgtagatt gattcaaact gcagatagta ttgccaatga tggatataat gatgaacttg
    17581 aactagatgc gattttaagt gatgcagaac gtcgaatttt agagctatca tcttctcgtg
    17641 aaagcgatgg ctttaaagac attcgagacg tcttaggaca agtatatgaa acagctgaag
    17701 agcttgatca aaatagtggt caaacaccag gtattcctac aggatatcga gatttagacc
    17761 aaatgacagc agggttcaac cgaaatgatt taattatcct tgcagcgcgt ccatctgtag
    17821 gtaagactgc gttcgcactt aatattgcac aaaaagttgc aacgcatgaa gatatgtata
    17881 cagttggtat tttctcacta gagatgggtg ctgatcagtt agccacacgt atgatttgta
    17941 gttctggtaa tgttgactca aaccgcctaa gaaccggtac tatgactgag gaagattgga
    18001 gtcgttttac tatagcggta ggtaaattat cacgtacgaa gatttttatt gatgatacac
    18061 cgggtattcg aattaatgat ctacgttcta aatgtcgtcg attaaagcaa gaacatggct
    18121 tagacatgat tgtaattgac tacttacagt tgattcaagg tagtggttca cgtgcgtcgg
    18181 ataacagaca acaagaagtt tctgaaatct ctcgtacatt aaaagcatta gctcgtgaat
    18241 tagaatgtcc agttattgca ttgagtcagt tatctcgtgg tgtagaacaa cgacaagata
    18301 aacgtccaat gatgagtgat attcgtgaat ctggttcaat tgagcaagat gccgatatcg
    18361 ttgcattctt ataccgtgat gattactata accgtggcgg cgatgaagat gatgacgatg
    18421 atagtggttt tgagccacaa acgaatgatg aaaacggtga aattgaaatc atcattgcta
    18481 agcaacgtaa tggtccaaca ggcacagtta agttacactt tatgaaacaa tacaataaat
    18541 ttacagatat cgattatgca catgctgata tgatgtaaaa aaagtttttc cgtacaataa
    18601 tcatgctaat gataaaattg tacggttttt attttgttct gaatgcgttg atatatgtta
    18661 agtttgtgta tttaaaacga cttaaaaaca ccgtaaatat tgctgtaaaa gggtttttga
    18721 agcatttcag catatttatg aaaatgattt ttcttttaga atgcaagaaa atcaatgttc
    18781 gatttttatt tgcattatgg tctcgatatt ggtagaatat caaatggtta aatgagaaaa
    18841 acttggaggt gctcacatgt catcaatcgt agtagttggg acacaatggg gagacgaagg
    18901 aaaaggaaag attacggatt tcttggcaga acagtcagat gttatcgcgc gtttttcagg
    18961 tggtaataat gcaggccata ccattcaatt tggcggagaa acatataaat tacatttagt
    19021 accatctggt atcttttata aagataaatt agcggtaatc ggtaacggtg tcgttgttga
    19081 tccagttgca ctattaaaag aattagacgg attaaatgaa cgtggcattc ctacaagtaa
    19141 tttacgtatt tctaatcgtg cgcaagtgat tttaccatat cacttagcac aagatgaata
    19201 tgaagaacgt ttacgcggtg acaataagat tggtacaact aaaaaaggta tcggtccagc
    19261 atatgtagac aaagttcaac gtatcggaat tcgtatggca gatttacttg aaaaagaaac
    19321 gtttgaaaga ttattaaaat caaacattga atataagcaa gcatatttca aaggtatgtt
    19381 taatgaaact tgcccatcat ttgatgaaat ctttgaagaa tactatgcag caggtcaacg
    19441 cttaaaagaa tttgtaacag atacatcaaa aatcttagac gatgcatttg tagcagatga
    19501 aaaagtactt ttcgaaggtg cgcaaggtgt aatgttagat atcgaccacg gtacatatcc
    19561 attcgttaca tcaagtaacc caattgcagg taatgttact gtaggtacag gtgtaggtcc
    19621 tacattcgtt tcaaaggtaa ttggtgtatg taaagcttat acatcacgtg ttggtgatgg
    19681 tccattccct actgaattat tcgatgaaga tggacatcat attagagaag ttggtcgtga
    19741 atacggtaca acaacaggac gtccacgtcg tgtaggttgg tttgattcag ttgtattacg
    19801 tcactctcgt cgtgttagtg gtattacaga tttatctatt aactcaattg atgttttaac
    19861 aggactagac acagtgaaaa tttgtacagc ttatgaatta gacggtaaag aaattactga
    19921 gtacccagca aacttagatc aattaaaacg ttgtaaacca atctttgaag agttaccagg
    19981 ttggacagaa gacgtaacaa gtgtgcgtac tttagaagaa ttacctgaaa atgcacgtaa
    20041 atatttagag cgtatttcag aattatgtaa tgtacaaatt tctatcttct cagttggtcc
    20101 agatagagaa caaacaaacc tattaaaaga attgtggtag aactttatat aagtcataca
    20161 caatgattat aaatacatga gccttctatc tttattggta ggaggctttt gttatgtttg
    20221 cttctgtatc gattcgatta attagataaa aattactaac gtaaaggtga tatttgctag
    20281 tcataattta aaagattaga tgatatttaa cgaaaattaa gatgaaatgc ttgaatgtaa
    20341 gaagtctgat gtcgaaaata gctattaaaa tagagtagac gtaagcgtgg atgaaagtat
    20401 ctaaaataga aaaaattcaa aaatagcgta attattataa taaatggaca gccaataaaa
    20461 tgcaattttt cacttataac attcttcaaa aaataatagc aaaattatgt aaaaaatatc
    20521 ttgtcatggc aagattggct gtgctataat ctatcttgtg cttaagaacg gctccttggt
    20581 caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgagtcc cgtaggagtc
    20641 accatttttt aggtctcgta gtgtagcggt taacacgcct gcctgtcacg caggagatcg
    20701 cgggttcgat tcccgtcgag accgtacaaa tgcctatcca agaggatagg cattttttta
    20761 cgtttaaaat tatatcaatt gaagacaaat ggacgaatga taaacaaaat gatttatcta
    20821 ttcgtccatt ttatttataa tgtataaact tctagtaatt cagttgcaat gtattaaaga
    20881 caaatgtaga aaacaaattt tttaattaga ttaccaccta tatttattaa catacttctt
    20941 catagtgata tgccaatagc atccacctaa cataaatata atagtattta ttttcctcat
    21001 atccacacca ataggaatcc cgtttttttc acacctttaa tagtataaaa tgatttcgtg
    21061 gtcatattat agggaaagac taattattat cacaatacgc cgtgagaaac taataaaaaa
    21121 tattaatata taagtttata ttggaaaata gaattaatag cttataaatg gtaaattata
    21181 taataggtta ctatacgtta taagacggaa aatgcgcaca ataacaaaaa tagtaagcga
    21241 catcctgtga ttttttacac aaacataaac gataaagaac aaaaaatgat aaaataatat
    21301 taatgattta agaaaagagg tttatgcaaa tggctagaaa agttgttgta gttgatgatg
    21361 aaaaaccgat tgctgatatt ttagaattta acttaaaaaa agaaggatac gatgtgtact
    21421 gtgcatacga tggtaatgat gcagtcgact taatttatga agaagaacca gacatcgtat
    21481 tattagatat catgttacct ggtcgtgatg gtatggaagt atgtcgtgaa gtgcgcaaaa
    21541 aatacgaaat gccaattata atgcttactg ctaaagattc agaaattgat aaagtgcttg
    21601 gtttagaact aggtgcagat gactatgtaa cgaaaccgtt tagtacacgt gaattaatcg
    21661 ctcgtgtgaa agcgaactta cgtcgtcatt actcacaacc agcacaagac actggaaatg
    21721 taacgaatga aatcacaatt aaagatattg tgatttatcc agacgcatat tctattaaaa
    21781 aacgtggcga agatattgaa ttaacacatc gtgaatttga attgttccat tatttatcaa
    21841 aacatatggg acaagtaatg acacgtgaac atttattaca aacagtatgg ggctatgatt
    21901 actttggcga tgtacgtacg gtcgatgtaa cgattcgtcg tttacgtgaa aagattgaag
    21961 atgatccgtc acatcctgaa tacattgtga cgcgtagagg cgttggatat ttcctccaac
    22021 aacatgagta gaggtcgaaa cgaatgaagt ggctaaaaca actacaatcc cttcatacta
    22081 aacttgtaat tgtttatgta ttactgatta tcattggtat gcaaattatc gggctgtatt
    22141 ttacaaataa ccttgaaaaa gagctgcttg ataattttaa gaagaatatt acgcagtacg
    22201 ctaaacaatt agaaattagt attgaaaaag tatatgacga aaagggctcc gtaaatgcac
    22261 aaaaagatat tcaaaattta ttaagtgagt atgccaaccg tcaagaaatt ggagaaattc
    22321 gttttataga taaagaccaa attattattg cgacgacgaa gcagtctaac cgtagtctaa
    22381 tcaatcaaaa agcgaatgat agttctgtcc aaaaagcact atcactagga caatcaaacg
    22441 atcatttaat tttaaaagat tatggcggtg gtaaggaccg tgtctgggta tataatatcc
    22501 ccgttaaagt cgataaaaag gtaattggta atatttatat cgaatcaaaa attaatgacg
    22561 tttataacca attaaataat ataaatcaaa tattcattgt tggtacagct atttcattat
    22621 taatcacagt catcctagga ttctttatag cgcgaacgat taccaaacca atcaccgata
    22681 tgcgtaacca gacggtcgaa atgtccagag ggaactatac gcaacgtgtg aagatttatg
    22741 gtaatgatga aattggcgaa ttagctttag catttaataa cttgtctaaa cgtgtacaag
    22801 aagcgcaggc taatactgaa agtgagaaac gtagactgga ctcagttatc acccatatga
    22861 gtgatggcat tattgcaaca gaccgccgtg gacgtattcg tatcgtcaat gatatggcac
    22921 ttaagatgct tggtatggcg aaagaagaca tcatcggtta ttacatgtta agtgtattaa
    22981 gtcttgaaga tgaatttaaa cttgaagaaa ttcaagagaa taatgatagt ttcttattag
    23041 atttaaatga agaagaaggt ctaatcgcac gtgttaactt tagtacgatt gtgcaggaaa
    23101 caggatttgt aactggttat atcgctgtgt tacatgacgt gactgaacaa caacaagttg
    23161 aacgtgagcg tcgtgaattt gttgccaatg tatcacatga gttacgtaca ccgttaacat
    23221 caatgaatag ttacattgaa gcacttgaag aaggcgcatg gaaagatgaa gaactagcac
    23281 cacaattttt atctgtaact cgtgaagaaa cagaacgaat gattcgactg gtcaatgact
    23341 tgctacagtt atctaaaatg gataatgagt ctgatcaaat caacaaagaa attatcgact
    23401 ttaacatgtt cattaataaa attattaatc gacatgaaat gtctgcgaaa gatacaacat
    23461 ttattcgaga tattcctaaa aagacgattt tcacagaatt tgatcctgat aaaatgacgc
    23521 aagtatttga taatgtcatt acaaatgcga tgaaatattc tagaggcgat aaacgtgtcg
    23581 agtttcacgt gaaacaaaat ccactttata atcgaatgac gattcgtatt aaagataatg
    23641 gtatcggtat tcctatcaat aaagtcgata agatattcga ccgattctat cgtgtagata
    23701 aggcacgtac gcgtaaaatg ggtggtactg gattaggact agccatttcg aaagagattg
    23761 tggaagctca caatggtcgt atttgggcaa acagtgtaga aggtcaaggt acatctatct
    23821 ttatcacact tccatgtgaa gtcattgaag acggtgattg ggatgaataa taaggaacat
    23881 attaaatctg tcattttagc gctactcgtc ttgatgagtg tcgtattgac atatatggta
    23941 tggaactttt ctcctgatat tgcaaatgtc gacaatacag atagtaagaa gagtgaaacg
    24001 aaacctttaa cgacacctat gacagccaaa atggatacaa ctattacgcc atttcagatt
    24061 attcattcga aaaatgatca tccagaaggt acgattgcga cggtatctaa tgtgaataag
    24121 ctgacgaaac ctttgaaaaa taaagaagtg aagtccgtgg aacatgttcg tcgtgatcat
    24181 aacttgatga ttcctgattt gagcagtgat tttacattat tcgattttac gtatgattta
    24241 ccattatcaa catatcttgg ccaagtactg aacatgaatg cgaaagtacc aaatcatttc
    24301 aatttcaatc gtttggtcat agatcatgat gctgatgata atatcgtgct ttatgctata
    24361 agtaaagacc gccacgatta cgtaaaatta acaactacaa cgaaaaatga tcatttttta
    24421 gatgcattag cagcagtgaa aaaagatatg caaccataca cagatatcat cacaaacaaa
    24481 gatacaattg atcgtacgac gcatgttttt gcaccaagta aacctgagaa gttaaaaaca
    24541 tatcgcatgg tatttaacac gattagtgtt gagaaaatga atgctatact atttgacgat
    24601 tcaaccatcg ttcgtagttc aaagagtggt gttactactt acaacaataa tacaggtgtc
    24661 gcaaactata acgataaaaa tgaaaaatat cattataaaa acttgtccga agatgaagca
    24721 agttccagca aaatggaaga aacgattcca ggaacctttg attttattaa tggtcatggt
    24781 ggtttcttaa acgaagactt cagattgttt agtacgaata atcagtcagg cgagctaaca
    24841 tatcaacgtt tccttaatgg ttatccaacg tttaataaag aaggctctaa tcaaattcaa
    24901 gtcacttggg gtgaaaaggg cgtctttgat tatcgtcgtt cgttattgcg caccgatgta
    24961 gtgttaaata gtgaggataa taaatcgttg ccgaaattag agtctgtacg ttcaagcttg
    25021 gcaaacaata gtgatattaa ttttgaaaaa gtaacgaaca ttgctatcgg ttacgaaatg
    25081 caggataact cagatcataa tcacattgaa gtgcagatta acagtgaact cgtaccgcgt
    25141 tggtatgtag aatatgatgg cgaatggtat gtttataacg atgggaggct tgaataaatg
    25201 aactggaaac ttacaaagac acttttcatt ttcgtgttta ttcttgtcaa catcgtgtta
    25261 gtatcgattt atgttaataa agtcaatcgc tcacacatta atgaagttga gagtaacaat
    25321 gaagttaatt ttcagcaaga agaaattaaa gtaccggcta gtatattgaa taaatcagtt
    25381 aaaggtataa aattagagca aattacgggg cgttcaaaag actttagttc taaagctaag
    25441 ggtgattcgg atttgaccgc agcagatggt ggaaaattat tgaatgcgaa cattagtcaa
    25501 tcggtaaagg tcagtgacaa taacttaaaa gatttgaaag attatgttaa caaacgtgtg
    25561 ttcaaaggtt cagaatatca attaagtgaa attagttctg gttctgtaaa atacgaacaa
    25621 acgtatgata atttcccgat tttgaataat agtaaagcga tgttgaactt taatatagaa
    25681 gataacaaag cggctagtta taaacaatca atgatggatg acattaagcc cacagatggt
    25741 gctgataaaa agcatcaagt gattggtgtg agaaaagcaa tcgaggcatt atattataat
    25801 cgttacttga aaaaaggtga tgaagtcatt aatgctagac tcggttacta ctcagtcgtg
    25861 aacgaaacga atgttcaatt gttacaacca aactgggaaa ttaaagtgaa gcatgacggt
    25921 aaggataaaa cgaatactta ctatgtcgaa gctacaaata ataaccctaa aattattaat
    25981 cattaatatg aatcataata agctagcatt gcaaactcat gagatatgag aagtggtgct
    26041 agctttttgc tggtatgggt tttcatggct gacatttctg tgtctctaac gtgcacactt
    26101 attcgtttat taagtagtac cgcattgtaa aattaccgta actgctattt taaaaacttt
    26161 agtatttgtc taatcattgt tataataatt aagaaattca ttgcacgtga ttatcaaaat
    26221 ttaaatataa gaaaccggtc gatgaactaa agttacataa taggaaaggt atacaaaaca
    26281 gctaatatac tgatagtttc tgtagggaaa atcgtatatt tgcactgatg tatattgcag
    26341 tcatatagag aaagtgactg tttaaagaga aaggatgagc cgcttgatac gcatgagtgt
    26401 attagcaagt ggtagtacag gtaacgccac ttttgtagaa aatgaaaaag gtagtctatt
    26461 agttgatgtt ggtttgactg gcaagaaaat ggaagaattg tttagtcaaa ttgaccgtaa
    26521 tattcaagat ttaaatggta ttttagtaac ccatgaacat attgatcata ttaaaggttt
    26581 aggtgttttg gcgcgtaaat atcaattgcc aatttatgcg aatgaaaaga cttggcaagc
    26641 tattgaaaag aaagatagtc gcatccctgt ggatcagaaa ttcattttta atccttatga
    26701 aacgaaatct attgcaggtt ttgatgttga atcgtttaac gtgtcacatg atgcgataga
    26761 tccgcaattt tatattttcc ataataacta taagaagttt acgattttaa cggatacggg
    26821 ttacgtgtct gatcgtatga aaggtatgat acgtggaagc gatgcgttta tttttgaaag
    26881 taatcatgac gtcgatatgt tgagaatgtg tcgttatcca tggaagacga aacaacgtat
    26941 tttaggcgat atgggtcatg tatctaatga ggatgcgggt catgcgatga cagacgtgat
    27001 tacaggtaac acgaaacgta tttacttatc gcatttatca caagataata acatgaaaga
    27061 tttggcgcgt atgagtgttg gccaagtatt gaacgaacac gatattgata cggaaaaaga
    27121 agtattgcta tgtgatacgg ataaagctat tccaacgcca atatatacaa tataaatgag
    27181 agtcacccga taaagttcca cactgctgtg agacgacttt atcgggtgct tttttatgtt
    27241 gttggtggga atggctgttg ttgagattaa tcggcttgat tgaaatgtaa aaaataattc
    27301 gatattaaat gtaatttata aataatttac ataaaatcaa tcattttaat ataaggatta
    27361 tgataatata ttggtgtatg acagttaatg gagggaacga aatgaaagct ttattactta
    27421 aaacaagtgt atggctcgtt ttgcttttta gtgtgatggg attatggcat gtctcgaacg
    27481 cggctgagca gcatacacca atgaaagcac aagcagtaac aacgatagac aaagcaacaa
    27541 cagataagca acaagtaacg ccaaccaagg aagcggctca tcattatggt gaagaagcgg
    27601 caaccaacgt atcagcatca gcacagggaa cagctgatga aataaacaat aaagtaacat
    27661 tcaacgcacc atctaacaaa ccatctacag cagtttcaac aacagtaaac gaaacgcacg
    27721 atgtagatgc acaacaagcc tcaacacaaa aaccaactca atcagcaaca ttcaaattat
    27781 caaatgctaa aacagcatca ctttcaccac gaatgtttgc tgctaatgca ccacaaacaa
    27841 caacacataa aatattacat acaaatgata tccatggccg actagccgaa gaaaaagggc
    27901 gtgtcatagg tatggctaaa ttaaaaacag taaaagaaca agaaaagcct gatttaatgt
    27961 tagacgcagg agacgccttc caaggtttac cactttcaaa ccagtctaaa ggtgaagaaa
    28021 tggctaaagc gatgaatgca gttggttatg atgctatggc agtcggtaac cacgaatttg
    28081 actttggata cgaccagttg aaaaagttag agggtatgtt agacttcccg atgttaagta
    28141 ctaacgttta taaagatggg aaacgcgcgt ttaagccttc aacgattgta acgaaaaatg
    28201 ggattcgtta tggaattatt ggcgtaacga caccagaaac aaagacgaaa acaagacctg
    28261 aaggcattaa aggtgttgaa tttagagatc cattacaaag tgtgacagca gaaatgatgc
    28321 gtatttataa agacgtagat acatttgttg ttatatctca tttaggtatt gatccttcaa
    28381 cacaagaaac atggcgtggc gattacttag tgaaacaatt aagtcaaaat ccacaattga
    28441 agaaacgtat tacagtcatt gatggtcatt cacataccgt acttcaaaat ggtcaaattt
    28501 ataacaatga tgcattagca caaacaggta cagcacttgc gaatatcggt aagattacat
    28561 ttaattaccg caatggagag gtatcgaata ttaagccgtc attgattaat gttaaagacg
    28621 ttgaaaatgt aacaccgaac aaagcattag ctgaacaaat taatcaagct gatcaaacat
    28681 ttagagcaca aacagcagag gttattattc caaataatac gatcgatttc aaaggagaaa
    28741 gagatgacgt tagaacgcgt gaaacaaatt taggaaacgc gattgcagat gctatggaag
    28801 cgtatggcgt taagaatttc tctaaaaaga ctgactttgc cgtgacaaat ggtggaggta
    28861 ttcgtgcctc tatcgcaaaa ggtaaagtga cacgctatga tttaatctca gtattaccat
    28921 ttggaaatac gattgcgcaa attgatgtaa aaggttcaga cgtctggaca gcttttgaac
    28981 atagtttagg cgcaccaaca acacaaaaag atggtaagac agtattaaca gcgaatggtg
    29041 gtttactaca tatctctgat tcaattcgtg tttactatga tatgaataaa ccatctggca
    29101 aacgaattaa cgctattcaa attttaaata aagagacagg taagtttgaa aatattgatt
    29161 taaaacgtgt atatcacgta acgatgaatg acttcacagc atcaggtggc gacggatata
    29221 gtatgttcgg tggccctaga gaagaaggta tttcattaga tcaagtacta gcaagttatt
    29281 taaaaacagc taacttagct aagtatgata cgacagaacc acaacgtatg ttattaggta
    29341 aaccagcagt aagtgaacaa ccagctaaag gacaacaagg tagcaaaggt agtgagtctg
    29401 gtaaagatac acaaccaatt gataaagaca aagtgatgaa tccagcgaaa caaccagctt
    29461 caggtaaagt tgtgttgtta ccagcgtata gaggaactgt tagtagtggt agagaaggtt
    29521 ctgatcgcgc attggaagga actgctgtat caagtaagag tgggaaacaa ttggctagca
    29581 tgtcagcgcc taaaggtagc gcacatgaga aacagttacc aaaaactgga actgatcaaa
    29641 gttcaagccc agcagcgatg tttgtattag taacaggtat aggtttaatc gcgactgtac
    29701 gacgtagaaa agctagctaa aatatattga aaacaatact actgtatttc ttaaataaga
    29761 ggtacggtag tgttttttta tggaaaaaag ctataaccgt tgataaatat gggatataaa
    29821 aacggggcta agtaataaga catcaaggta tttatccaca gaaatgggga tagttatcca
    29881 gaattgtgta caatttaaag agaaataccc acaataccca cagagttatc cacaaataca
    29941 caagttatac actgaaaatt gggcatgaat atcagaaaaa tatcaaaaac tgcaaaaaaa
    30001 cttggtataa taagagggaa cagtgtgaac aagttaataa cttgtggata actggaaagt
    30061 tgataacaat ttggaggacc aaacgacatg aaaatcacca ttttagctgt agggaaacta
    30121 aaagagaaat actggaagca agccatagca gaatatgaaa aacgtttagg cccatacacc
    30181 aagatagaca tcatagaagt tccagacgaa aaagcaccag aaaatatgag cgacaaagaa
    30241 atcgagcaag taaaagaaaa agaaggccaa cgaatactag ccaaaatcaa accacaatcc
    30301 acagtcatta cattagaaat acaaggaaag atgctatctt ccgaaggatt ggcccaagag
    30361 ttgaaccatc gcatgaccca aggccaaagc gactttgtat tcgttattgg cggatcaaac
    30421 ggcctgcaca aagacatctt acaacgcagt aactacgcac tatcattcag caaaatgaca
    30481 ttcccacatc aaatgatgcg ggttgtgtta attgagcaag tgtacagagc atttaagatt
    30541 atgcgtggag aagcatatca taaatgatgc ggttttttca gccgcttcat aaagggattt
    30601 tgaatgtatc agaacatatg aggtttatgt gaattgctgt tatgttttta agaagcttat
    30661 cataagtaat gaggttcatg atttttgaca tagttagcct ccgcagtctt tcatttcaag
    30721 taaataatag cgaaatattc tttatactga atacttatag tgaagcaaag ttctagcttt
    30781 gagaaaattc tttctgcaac taaatatagt aaattacggt aaaatataaa taagtacata
    30841 ttgaagaaaa tgagacataa tatattttat aataggaggg aatttcaaat gatagacaac
    30901 tttatgcagg tccttaaatt aattaaagag aaacgtacca ataatgtagt taaaaaatct
    30961 gattgggata aaggtgatct atataaaact ttagtccatg ataagttacc caagcagtta
    31021 aaagtgcata taaaagaaga taaatattca gttgtaggga aggttgctac tgggaactat
    31081 agtaaagttc cttggatttc aatatatgat gagaatataa caaaagaaac aaaggatgga
    31141 tattatttgg tatatctttt tcatccggaa ggagaaggca tatacttatc tttgaatcaa
    31201 ggatggtcaa agataagtga tatgtttccg cgggataaaa atgctgcaaa acaaagagca
    31261 ttaactttat cttccgaact caataaatat attacatcaa atgaatttaa tactggaaga
    31321 ttttattacg cagaaaataa agattcatct tatgatttaa aaaatgatta tccatcagga
    31381 tattctcatg gatcaataag attcaaatat tatgatttga atgaaggatt cacagaagaa
    31441 gatatgctag aggatttaaa gaaattttta gaactattta atgaattagc ttcaaaagtt
    31501 acaaaaacat cctatgatag cttggtcaat agcatagacg aaatacagga agacagcgaa
    31561 attgaagaaa ttagaacagc acaaaaagat aagacactca aggaagtgga agcacctaaa
    31621 ggaataattc caaaatataa aaaaggtgta tcaaagacta ctaaaaatga ttcagaaatt
    31681 gaaaaatcaa ataaagagaa taaattaacc ggtaaagttg gagaaaaatt agcgctaaat
    31741 tactttaatg agctaattga taataaaata gacgaagata agaaagaaca gtttaggaat
    31801 attttaaatg ataatccagg ctctcaacac ggtcatggct atgatttagt agcttttgat
    31861 ccaacaaata cagataaagc tgtagaaaaa tttattgaaa ttaaaacatc tacatcttct
    31921 agtattgagg aaccattttt tatgtcgcta aatgaaatgt ttgctatgaa agaatataag
    31981 cagaaatatt taatattaag aatatttaat gtttccggta aagaaccaca attttatttt
    32041 atagatccat atgcaaatta ttctgaattt aaagatgtag atgatctcat tgacaaagta
    32101 tttaatgtag aagctattca gtataaagtt tttggcgaaa aatgattact tgaacaagag
    32161 ctaaaataaa attgtgatct aataaaaata gaaactgtaa tttaaataaa actttctaaa
    32221 taagctaact gataaaaaat cagtttgtcc acagtctgaa acaagattcc tatattcttt
    32281 aggaatcttg ttttttctat ttttatggtg ataaagagca gataagataa tgtgtaataa
    32341 tcacaaaaaa gttaaatatt ttaaggcttg tttaattatt aatgatttta tatataaaga
    32401 gcagtataat aaagttgtta atatattatg aataatattc aagtaatttt attgtttttt
    32461 aatttgtcga tatttaagtt gagttaaatt taaagggtgt aatttgtttt acaatgatga
    32521 agataattag tctatcaaaa taaaggggtt gggactgtta tgagtgataa tttgtcatta
    32581 ttcattgact atatcaatga taatataatc tatggtagtg aaatcaaacg ggagaaatta
    32641 gagaatttat ttaatcaatt tgctataaaa aatgttgaaa agaacattgt ctatgatgaa
    32701 ctgaaatctt tagatattac aatcattgag tcacaggatt catataaaaa taaattgaag
    32761 agattatttt cggttctgtt gcaaagtaaa aaaatatagc taaccactaa tttatcatgt
    32821 cagtgttcgc ttaacttgct agcatgatgc taatttcgtg gcatggcgaa aatccgtaga
    32881 tctgaagaga cctgcggttc tttttatata gagcgtaaat acattcaata ccttttaaag
    32941 tattctttgc tgtattgata ctttgatacc ttgtctttct tactttaata tgacggtgat
    33001 cttgctcaat gaggttattc agatatttcg atgtacaatg acagtcaggt ttaagtttaa
    33061 aagctttaat tactttagcc attgctacct tcgttgaagg tgcctgatct gtaattacct
    33121 tttgaggttt accaaattgt ttaatgagac gtttgataaa cgcatatgct gaatgattat
    33181 ctcgttgctt acgcaaccaa atatctaatg tatgtccctc tgcatcaatg gcacgatata
    33241 aatagctcca ttttcctttt attttgatgt acgtctcatc aatacgccat ttgtaataag
    33301 cttttttatg ctttttcttc caaatttgat acaaaattgg ggcatattct tgaacccaac
    33361 ggtagaccgt tgaatgatga acgtttacac cacgttccct taatatttca gatatatcac
    33421 gataactcaa tgtatatctt agatagtagc caacggctac agtgataaca tccttgttaa
    33481 attgtttata tctgaaatag ttcatacaga agactccttt ttgttaaaat tatactataa
    33541 attcaacttt gcaacagaac cgtattatgg aatagagatg ttggtaacat ttatacagga
    33601 tcattatact taagtttaat ttcgttatta cagaaccaca cattccaacc agaagagaaa
    33661 gtatgtctat ttagttatgg ttcaggagca gtaggagaaa tctttagtgg ttcaatcgtt
    33721 aaaggatatg acaaagcatt agataaagag aaacacttaa atatgctaga atctagagag
    33781 caattatcag tcgaagaata cgaaacattc tttaacagat ttgataatca agaatttgat
    33841 ttcgaacgtg aattgacaca agatccatat tcaaaagtat acttatacag tatagaagac
    33901 catatcagaa catataagat agagaaataa actagtggcc gattgtgctt gatgagcttg
    33961 ggacataaat cctaactcga aataaataag catatcacta aactgatttt ttaaagttta
    34021 cagtgatatg cttatttttt tatcttacga ttttgtacgt gcatgcttgc ctaggggtat
    34081 ggctcgagcc attagtctct cgcacatact attccctcag gcgtcagcac ttacaaaatc
    34141 ggttgtaatt ttcattttta tacgcattct tactgagatt atactaataa gaggaatagt
    34201 aaaagca
//
LOCUS       DAHHRR010000016        34027 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.016, whole genome shotgun sequence.
ACCESSION   DAHHRR010000016 DAHHRR010000000
VERSION     DAHHRR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.016"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(1..113)
                     /locus_tag="NBI42_000637"
                     /pseudo
     CDS             complement(1..113)
                     /locus_tag="NBI42_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
     gene            264..1262
                     /locus_tag="NBI42_000638"
     CDS             264..1262
                     /locus_tag="NBI42_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1777079.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHHDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNNMMIVAPYDMMANMSTRVDGFVDTLRTNQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKISLTLLLQRLQHLDKSIDMIELPTTLKIRATTGHHFSN"
     gene            1494..2375
                     /gene="rbsU"
                     /locus_tag="NBI42_000639"
     CDS             1494..2375
                     /gene="rbsU"
                     /locus_tag="NBI42_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HCD1777080.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVILLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2390..2794
                     /gene="rbsD"
                     /locus_tag="NBI42_000640"
     CDS             2390..2794
                     /gene="rbsD"
                     /locus_tag="NBI42_000640"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCD1777081.1"
                     /translation="MKKSAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2822..3736
                     /gene="rbsK"
                     /locus_tag="NBI42_000641"
     CDS             2822..3736
                     /gene="rbsK"
                     /locus_tag="NBI42_000641"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCD1777082.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTTEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDIINAKDAIINADFVVAQLEVPIPAIIAAFEIAKSHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAIN
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3833..3967)
                     /locus_tag="NBI42_000642"
     CDS             complement(3833..3967)
                     /locus_tag="NBI42_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777083.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESSSIGLVENYF
                     "
     gene            3986..4747
                     /locus_tag="NBI42_000643"
     CDS             3986..4747
                     /locus_tag="NBI42_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1777084.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKAATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDISKKALEKAQENISAAGLGSYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTLKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(5263..6699)
                     /locus_tag="NBI42_000644"
     CDS             complement(5263..6699)
                     /locus_tag="NBI42_000644"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCD1777085.1"
                     /translation="MTNLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDFGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYNRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6715..7506)
                     /locus_tag="NBI42_000645"
     CDS             complement(6715..7506)
                     /locus_tag="NBI42_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1777086.1"
                     /translation="MGNKYKDYAQDILTAVGGVENIVNVSYDTVRMTIHMHHAIPSTA
                     NEVTQIDGVASVDENETQLVIVFNEEVKYVYQQLQLLMDDAKHQEDTNHDAVDTQETE
                     QKAQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     VSMIVPTKHAVGIQSEDGVDIVIHIGVNTVDLEGKGFKCFVKQNDRVEAGQTLLQFDQ
                     QYIQEQGYNSDVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7653..8357
                     /locus_tag="NBI42_000646"
     CDS             7653..8357
                     /locus_tag="NBI42_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1777087.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8583..10874)
                     /locus_tag="NBI42_000647"
     CDS             complement(8583..10874)
                     /locus_tag="NBI42_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HCD1777088.1"
                     /translation="MSMQSSYKRLVKVLLTILVIVFSILLVGGWYIFKNEAPRPTKIV
                     DQQGHTLVTKGELISGQAIYEKYGLTDYGSYLGNGSYLGPDYTAEALHHYLIGMRKYY
                     AQDIYHKQLHKLNHAELAVVKDKVMKEIRINRYSEKENQLVLTPGQVYGLKYLQDYYK
                     KEFVNNPKQVGLNENMIKQFQNDDYMVAGNKVEHLSQFFFWGAWLSSTDRPGKTFSYT
                     NNWPYDVDAGNTLPSAGILWTAISVTLLIAGLAFIIYIQKRYEFDMKPTYESERELPK
                     IEVDSKITDSQRKVGKYLVVVMLLFLVQILLGELLAHYYVENKFFGIEIQRLFPFNIA
                     KTWHVQLVIFWVATTWLAAGIYIVPKVLGKEPKKQGVLVDILFWALIIVVMGSMIGEW
                     GHILGWIDSHWWLFGHFGWEYIELGKFWQILFIVGMVLWVIILCRGFLPAIRNKVQAN
                     HDKRHLLTLLFVGAIAIPLFYLASLFIMPNSHVTFADYWRWWIVHLWVEGIFEAFAVV
                     LIGFLMVNMKLTTIETTIRALYFQLILLLGTGIVGMGHHYYWQGDHSIWLALGSCFSA
                     LEVVPLCLLIWEAYTHYRLYKKSDIAFPYKGTFIFLASTGLWNAIGAGALGFLINTPA
                     VNYFEHGTQWTAAHAHGSMAGVYGMFSIAIALYVFKNITKKEFWTPKVEKWIKISCWA
                     LNVGLAGMVFITLMPVGYIQLKDALEHGYWHSRLTSFYEQPLVKAIMWGRMPWDIIFT
                     VGVIILVVIFIRGYRHLKPKYRV"
     gene            complement(11215..11916)
                     /gene="lrgB"
                     /locus_tag="NBI42_000648"
     CDS             complement(11215..11916)
                     /gene="lrgB"
                     /locus_tag="NBI42_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HCD1777089.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVVIFF"
     gene            complement(11909..12352)
                     /gene="lrgA"
                     /locus_tag="NBI42_000649"
     CDS             complement(11909..12352)
                     /gene="lrgA"
                     /locus_tag="NBI42_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HCD1777090.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(12465..13205)
                     /locus_tag="NBI42_000650"
     CDS             complement(12465..13205)
                     /locus_tag="NBI42_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HCD1777091.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNSANAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPAYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(13208..14962)
                     /locus_tag="NBI42_000651"
     CDS             complement(13208..14962)
                     /locus_tag="NBI42_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1777092.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIAYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(15208..15882)
                     /gene="scdA"
                     /locus_tag="NBI42_000652"
     CDS             complement(15208..15882)
                     /gene="scdA"
                     /locus_tag="NBI42_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HCD1777093.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQISIEAASLEK
                     KNVDLNELLQRLNDIEQTNTPGSLNPKFLNVSSLIQYIQAAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGQLLVKMSDLTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(16037..17758)
                     /gene="tarS"
                     /locus_tag="NBI42_000653"
     CDS             complement(16037..17758)
                     /gene="tarS"
                     /locus_tag="NBI42_000653"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCD1777094.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLITLEILNADLEESHKDQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVLSKLRPLLHYARAKDIDNYRTVEESYRQGQYYHFDIVDGK
                     LNIQFNEGEPFFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKPYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISMKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMIMGMLSQTSENEVEILLSKDIKKRDFKNIVKLNTA
                     HMTYSLK"
     gene            complement(17791..19479)
                     /locus_tag="NBI42_000654"
     CDS             complement(17791..19479)
                     /locus_tag="NBI42_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1777095.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(19501..20526)
                     /locus_tag="NBI42_000655"
     CDS             complement(19501..20526)
                     /locus_tag="NBI42_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCD1777096.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(20519..21235)
                     /locus_tag="NBI42_000656"
     CDS             complement(20519..21235)
                     /locus_tag="NBI42_000656"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HCD1777097.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            complement(21514..22683)
                     /locus_tag="NBI42_000657"
     CDS             complement(21514..22683)
                     /locus_tag="NBI42_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1777098.1"
                     /translation="MIKTTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQHAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDNYVILLRMHYLVVTRVDEHDDFVKDV
                     SDYEDISDLYLISDVLITDYSSVMFDYGVLKRPQIFYAYDLEKYGDELRGFYMDYKKE
                     LPGPIVENQAALIETLKHIDITANDYVAARTAFYQKYCSLEDGYASQRICQTLFK"
     gene            complement(23306..25000)
                     /locus_tag="NBI42_000658"
     CDS             complement(23306..25000)
                     /locus_tag="NBI42_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1777099.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFLCRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKANYVLANEAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDETYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     YESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(25022..26047)
                     /locus_tag="NBI42_000659"
     CDS             complement(25022..26047)
                     /locus_tag="NBI42_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCD1777100.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKIYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(26040..26756)
                     /locus_tag="NBI42_000660"
     CDS             complement(26040..26756)
                     /locus_tag="NBI42_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCD1777101.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     DFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKIANAIIQGDIADD"
     gene            complement(27283..28326)
                     /locus_tag="NBI42_000661"
     CDS             complement(27283..28326)
                     /locus_tag="NBI42_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCD1777102.1"
                     /translation="MKALKLYGVEDLRYEDNEKPIIESANDIIVKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIAKSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTIPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            complement(28350..28496)
                     /locus_tag="NBI42_000662"
     CDS             complement(28350..28496)
                     /locus_tag="NBI42_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777103.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(28498..29553)
                     /locus_tag="NBI42_000663"
     CDS             complement(28498..29553)
                     /locus_tag="NBI42_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCD1777104.1"
                     /translation="MKAIVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKVQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGEN"
     gene            complement(29571..30830)
                     /locus_tag="NBI42_000664"
     CDS             complement(29571..30830)
                     /locus_tag="NBI42_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCD1777105.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQVICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQRVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(31057..31335)
                     /locus_tag="NBI42_000665"
     CDS             complement(31057..31335)
                     /locus_tag="NBI42_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1777106.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(31358..31825)
                     /locus_tag="NBI42_000666"
     CDS             complement(31358..31825)
                     /locus_tag="NBI42_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1777107.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLYQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDQAALATIINGYLEYN"
     gene            complement(31810..33906)
                     /locus_tag="NBI42_000667"
     CDS             complement(31810..33906)
                     /locus_tag="NBI42_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCD1777108.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLGNHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVINPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDNISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKDVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRIHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKETRGNGWDSN"
BASE COUNT        10827 a         6524 c         4984 g        11692 t
ORIGIN      
        1 tattctttaa aataatatct tttgtatttt tcatcacaat aaacatttta aaattcttgt
       61 tatcataatc attaaatgtt atttaacctt aataatatat tctctcgtct caaccttaat
      121 cgcatactat agacgtctgt ttgtagacaa taaaagtcat gcacctcttc atatgtcatc
      181 aaatgcttat catgatatga tgaatataat aatcgggtat ataactgtat gattaattac
      241 acaataaaga gaaggaatat aatatgaaaa aagtgtcaat taaagatgtt gctagagaag
      301 ctggtgtatc agttacaact gtgtcacata ttttaaatca tcatgatagt cgtttttccg
      361 caacaacgat aaaaaacgta catgctgttt cagaacgttt aggctatgcc cctaacaaac
      421 atgcaaaaca attgcgcggc agtaaaattc aaactattgg cgtcattttg cctagcttaa
      481 caaatccgtt tttctcagca ctgatgcaaa gtattcatga ccacaaacca tctgatgttg
      541 atttatgctt tttaacatct acagcaactg atttgtatga caatattaaa catttaattg
      601 atcgaggtat tgacggatta attatcgcac aatacatttc atccccggac gccctaaata
      661 actatctaaa gaaacatcat gtaccttatg tcgtactgga tcaaaatgac catcaaggct
      721 atacagattt tgttcggact aatgaatatc aaggcggaca acttgcagca caacatttaa
      781 tagaactcgg tcacaacaat atgatgattg ttgcaccata tgacatgatg gcgaatatgt
      841 cgactcgtgt cgatggattt gttgatactt tgcgcacgaa tcaattgccc gaaccacaaa
      901 tcgtccatac tgaattatct aaacgcggtg ggctaaccat tgttgatgac atcatggttc
      961 aatctgccac tgcaatcttc gctattaacg atgaactcgc tattggcatt ttacgaggac
     1021 taattgaaca tggcatcagt atcccgaaag atatctcatt aataggttat gacgacattg
     1081 attatgcagc gtacgtctcg ccacctttaa ctactgtggc acaacctata actgatattg
     1141 gcaaaatatc tttaacctta ttacttcaac gattacagca cttagataaa tccattgata
     1201 tgattgaatt accaacgact ttaaaaattc gtgcaacgac tggccatcat ttttcaaact
     1261 aactacgtat cttccttaat atactcatca tttttatgcc cttagcgttg ctttaatgct
     1321 gagggttttt aatcataatt atttcactaa gaaattaaaa taataatgta tgaattttta
     1381 aatgtgattt aaacgttttc agtttttatg aaaacgcatg cattttacaa ataaaaatgg
     1441 tacgatggca ctggtaaaac gttttactaa aaacaaatcg tgaggtgtat aacatgagca
     1501 ttgttgcatt acttatcggg ttaggcccct taattggctg gggcttcttc ccaacagtcg
     1561 cttcaaagtt tggtggtaaa cctgtacatc aaattatcgg tgctactgta ggtacgttaa
     1621 tcttcgctat tatattagcc gtagtcacat caagtggctt ccctactgga acaaatttac
     1681 tattcgcctt attatcaggt gcaggatggg gattcggaca aatcattaca tttaaagcgt
     1741 tcgaattagt cggttcatct cgtgccatgc ctgtcacaac agcattccaa ttattaggcg
     1801 catctttatg gggagtcttt gcattaggaa attggccggg cattggtcat aaaatcattg
     1861 gctttacagc tttagtcgtt attttaattg gtgcgcgtat gacagtttgg agtgaacgca
     1921 aagaagcaag taacgccaaa aatttacgtc gtgcagtgat tcttctgtta attggtgaat
     1981 ttggatactg gttatattca gccgcaccac aagcaacttc tattgatggc ctaactgcct
     2041 ttttacctca agcaatgggt atggtaattg ttgcagtcat ttatggcttt atgaatatga
     2101 aagcagagaa tccatttcgt aataaaatta cgtggttaca aattatttca ggtttcttct
     2161 ttgcatttgg tgccttaaca tatcttattt cagcacaacc taatatgaat ggtttagcaa
     2221 ctggatttat tctttctcaa acatccgttg tgcttgctac attaactggt atttatttct
     2281 taaaacaaca taaaacgtca aaagaaatgg ttattacaat tatcggctta gtactcattt
     2341 tagtagccgc ttctgttaca gtatttataa aataaggagt gtagatgtca tgaaaaaatc
     2401 agctgtttta aatgaacata tttcaaaagc aatcgcgaca attggtcatt ttgatttatt
     2461 aacgattaat gacgctggca tgccaattcc aaatgatcat cgccgtatcg acctagctgt
     2521 caccaaaaac ttaccacgct ttattgatgt cttagctaca gtattagaag aaatggaaat
     2581 ccaaaaaata tacttagcag aagaaataaa agaacataat cctacacaat tgcaacaaat
     2641 taaacaattg atttcatcgg aaatcgaaat cattttcatt cctcacgaag aaatgaaaag
     2701 taacttagct cacccattaa ataaaggtaa tattcgtact ggtgaaacaa cgccctactc
     2761 taatattgca ttagaatcga atgttacttt ttaaaaatta taacttgaaa ggagcataca
     2821 catgaccaac aaagttgtta ttttaggttc aacgaatgtc gatcaatttt taacagttga
     2881 aagatatgca caaccaggcg aaacattaca tgttgaagaa gcacaaaaag cattcggcgg
     2941 aggtaaaggt gccaaccagg ctattgccac tgcacgtatg caagcagaca caacatttat
     3001 tactaaaatt ggcactgatg gcgttgctga tttcatctta gaagatttta aagcggctca
     3061 tattgataca tcatatatta tcaaaacaac tgaagcaaaa acgggccaag cctttatcac
     3121 tgtgaatgca gaaggacaaa acaccatcta tgtttatggt ggtgcgaata tgacaatgac
     3181 acctgaagat attattaacg caaaagacgc tataatcaat gcagactttg tcgttgcaca
     3241 attagaagta cccatcccag ctattatagc tgcatttgaa attgccaagt cacatggtgt
     3301 gacgacagta ttaaatcctg caccagcgaa agcattacct aatgaattat tgtcactaat
     3361 cgatattatt gtgccaaacg aaacagaagc tgaattgtta tctgggatta aagtaactaa
     3421 tgaacaatct atgaaagaca atgctaatta ctttttatct ttaggcatta agactgtttt
     3481 gattacgtta ggtaagcaag gtacatattt tgctactaaa aatcaaagcc aacacatcga
     3541 agcttataaa gtaaatgcga ttgatacaac tgctgcaggc gacacattta ttggtgcatt
     3601 tgtcagtcgc ttaaacaagt cgcaagataa cttagcagat gctattaatt ttggtaataa
     3661 agcgagctca ctcactgtac aaaaacacgg cgcacaagca tctattcctc tactagaaga
     3721 agtaaatcaa gtttaaatga atcaaacaca gctatgatat gaaggtttag catataacat
     3781 gcaacattcg tatatcatgg ctgtgctttt tttctttata aaacatcatc tattagaaat
     3841 aattttccac taaacctatg cttgatgact cgtgtttagt tataaatgaa gtgacaattt
     3901 tttgtaatct ttttaacttc caaattatcg catataaata tgctatatta atgataataa
     3961 ttatcaatta acaggaggtt atgctatgtc caaagaagcc ggtcatacat ttttagctaa
     4021 attaggaaaa actcgcttac gtcctggtgg taaagcagca acagattggt taatacaaca
     4081 aggggcattt tcacaagata aacaagtgtt agaagtggca tgtaatatgt gtacgacatc
     4141 tatttatcta gctcatacat acggctgtca cattcaaggc gttgatataa gtaagaaagc
     4201 attagaaaaa gcacaggaaa atatttcagc agcaggtctt ggatcatata ttcaagttca
     4261 acaagcgaat gctgttaaat tgcccttcga tgacaatcaa ttcgatatcg ttttaaatga
     4321 agcaatgtta acaatgttac ccatcgccat aaaggaaaaa gcattacgcg agtactaccg
     4381 ggtcttaaag cctgggggta tcttgttaac acatgatatt gtcatcgtta atgaatcaca
     4441 tgccacacat gtagttaaat cattatctgc agcaattaat gtcaatgtct caccgcagac
     4501 gaaacttggc tggttagatt tatataatca agctggtttt aatcatgtgc attatcatac
     4561 tggtccaatg agtttaatga caccaaaagg tttaatttat gatgaaggta ttgtcggaac
     4621 tttaaagatt atcaacaatg ctttaaaaaa agaaaatcga ccaatgtttt gtaaaatgtt
     4681 taaaacgatg actaaactgc gtaaagatat gaattatatt acttttgtcg ctaaaaaaga
     4741 gcactaaata taatgccact aactgtactt tgtatctatg tttgactgtc actttaattt
     4801 ctttgtgaca ctaatcatct acttaacaat atcgttatcg ttgattagta ggtctcaatt
     4861 ttggttaaag acttttataa acactcaaac attaacacta tacatactta gttagtggca
     4921 ttattttttc ttaaaatttt aacatcacgg gattggaaca gaaatgttaa agggagtagg
     4981 acagaaatta tattttcaca aattttattt ctgtccctcc ccaactcgca ttgcctgttg
     5041 aaattggtgt tccaatttct ctatgttggg gccccgccaa ctcgcattgc ctgttgaatt
     5101 tcttttcgaa gttctctgtg ttggaggcct gccaactcgc acattattgt aaactgtatt
     5161 tccgccagct tctatgttgg ggatccgcca atataacact ctagggccta ctatattaat
     5221 ttatgtctcg ggctctaacc ctaaaatact tataacgtat ctttataatt tatctccatt
     5281 tgatgctatc acttgttgat accaatagaa cgaatctttt ttcaatcgtt ccatcgtgcc
     5341 atgaccatca ttatctcgat caacatatat taaaccataa cgtttcttca tttcaccggt
     5401 tgtaaatgaa acaatatcaa tgattcccca tggcgtataa ccgattaaat ctacaccatc
     5461 ttgatcaact gcttctatcg ctgctgtaat atgtgctttt aaatattcaa tgcgataatc
     5521 atcatgaata tgtccatcta ccacttcatc aactgcacca aaaccatttt ccacaataaa
     5581 aagtggcaac tgataacgat tatataacac attcaatgta tatcttaagc catctggatc
     5641 aatcgcccaa ccccaatcac tcgttgcgat atgcggattc tccacagaat gattcaaacc
     5701 accgttgacg atgttgtttt caactgtagt atcaacatca tgttttacag ccgtagacat
     5761 gtaataacta aagccaatat aatcaaccgt gccctgctgt aaaatactgt cgtccccttc
     5821 ttgccatcca aaatcatatc cttttcgtgc caacattttt ttagcatagc ttggataata
     5881 acctcttact tgaacatccg ggaaaaagaa acgtaagcga ttcgcaattt gtgcttccat
     5941 catatctttc ggatgacacg aatatggata aatgggtaca tgtgaaatca ttgttccaat
     6001 cttaaatttc ggattaatct ctttaccaag acgaaccgct aaagcactgg ctaaaagttc
     6061 atgatgtgct acttgataca agacttcttc gggattatca ttttctgtta atgctacccc
     6121 agaattcgtc cataaaaaga taggatttga tgtgtccatc tgattattaa tttcattaaa
     6181 cgtcatccaa tatgtcactt tatctttata tctttcaaat acaacacgcg caaaatgcac
     6241 aaaataatcg acaacttctc tatttctaaa tccaccataa tgtttcgcta aatgaagtgg
     6301 catctcaaaa tgtgataaag tcacaacagg ttcaatacct tgtgcaatta attcatcaaa
     6361 gatacgatca taaaaggcga gtccttcttc atttggcaca tcttcatccc catttggaaa
     6421 gatacgtgtc cacgcaatcg acgtacgtaa acatttcaat cccatttctt taaacaaggc
     6481 aatatcttcc ttataacgat gataaaaatc aataccttca tgatttggat aatagtgatt
     6541 ggggtctata gattctgtaa tctgacgtgc tttgccatgt gcaccactcg tcataacatc
     6601 aattacactt aatcctttgc cacctttatc atatccacct tcaaattgat ttgcggcaag
     6661 tgcgccaccc cacataaaat tttgcggtaa atttgtcatt attacaacac actcctagtt
     6721 tttaaatatt ttaaaaataa catcttcagt cgtaatgatt tcattcattg tcagttctac
     6781 ttttcctaaa tcggcagagt tgctaataac gacaataacg tctgagttat aaccttgttc
     6841 ctgaatatat tgttgatcga attgtaacaa cgtttgccct gcttcaacac gatcattttg
     6901 ctttacaaag cacttgaatc ctttaccttc caagtcaact gtattcacac caatatgaat
     6961 gactatgtcc acaccgtctt ctgattgaat accaactgca tgcttagttg gtacaatcat
     7021 agatactaag ccattgaacg gtgcgattac tttggattct tcatgagctt tgattgccaa
     7081 gccttcgcct accattttct ctctaaaaat tgaatctctt acttctttaa gtaaaatacg
     7141 acgaccagcg attggtgctt tcactaaaat tggggttgtt acttttactt gcgctttctg
     7201 ttctgtttct tgagtatcca cagcatcatg attcgtatct tcttgatgct tggcgtcgtc
     7261 cattaacaat tgtaattgtt gatatacata ttttacttct tcattaaata cgataactaa
     7321 ctgtgtttca ttttcatcaa ctgacgccac gccatctatt tgtgttactt cattagcagt
     7381 agaaggaatt gcatgatgca tatgaatggt catgcgtacc gtatcatacg aaacattaac
     7441 aatattttcg acacccccta cagctgtaag gatgtcttga gcataatctt tatatttatt
     7501 gcccataagt attaccccct caacttgata atagaatgga aatatatgac ttatcataat
     7561 tataattgtg ccggttcaat ttgtaaacgc cgatacaatt ataatatttt gtgctataat
     7621 aattacagac aaagtgaaac gaggacagaa tattgttaaa gtatgaacat attgctaagc
     7681 aacttaatgc gtttatacat caatctaatt tcaaacccgg tgataaattg ccaagcgtga
     7741 cgcaattaaa agaacgttat caagtaagta agagtactat cattaaagca ttaggcttat
     7801 tggaacaaga tggtttgatc tatcaagcac aaggcagtgg tatttatgtg agaaatattg
     7861 cagatgccaa tcgtatcaat gtttttaaga ccaatggttt ttctaaaagt ttaggcgaac
     7921 atcgaatgac aagtaaggta cttgttttta aggagattgc aacgccacct aaatctgtac
     7981 aagatgagct ccaattaaat gcagatgata ccgtctacta tttagagcga ttaagattcg
     8041 tggacgatga tgttttatgt atcgaatatt cttattatca taaagaaatc gtgaaatatt
     8101 taaatgatga tattgctaag ggctctatct tcgattattt agaatcaaac atgaaacttc
     8161 gtattggttt ttcagatatt ttctttaatg tagatcaact cacttcaagt gaagcttcat
     8221 tactacaatt gtctacaggt gaaccatgtt tacgttacca ccagactttt tatacaatga
     8281 ctggcaaacc ctttgattcg tctgacatcg tatttcatta tcgtcatgca cagttttata
     8341 ttcctagtaa aaagtaatac atacatcaaa acgtctatat tccagttatc aaccggagta
     8401 tagacgtttt ataggacata atttctttaa aaacagtgag aagtacattt tcaatcagaa
     8461 aattactttt tactgttctt tatatttaca taatctaaga acctatagtt tgaatcctat
     8521 tgtataatat ctcttcgtct caaacgttaa aacacactaa gatcactcag taatatgatg
     8581 atctatactc tgtattttgg ttttaagtgt ctgtatcccc ttataaaaat aactactaat
     8641 ataatgacac ctactgtgaa tataatgtcc caaggcattc taccccacat gattgctttt
     8701 acaagtggtt gttcataaaa tgacgttagt cttgaatgcc aataaccatg ttctaatgca
     8761 tctttaagtt ggatataacc tactggcatt aatgtaatga acaccatacc tgccaagcca
     8821 acatttaaag cccaacatga tattttaatc catttttcaa ctttcggtgt ccaaaattct
     8881 tttttagtaa tatttttaaa aacatacaac gcaattgcaa ttgagaacat accatacacg
     8941 ccagccatag aaccatgggc atgtgctgca gtccattgcg taccatgttc aaagtagtta
     9001 actgctggtg tgttaattaa aaagcctaat gctccagcac caattgcatt ccacaatcct
     9061 gttgaagcaa gaaaaataaa tgtacctttg taaggaaaag cgatgtcact tttcttatat
     9121 aagcgatagt gtgtgtatgc ttcccaaatt aataaacaaa gtggcacgac ttctaatgct
     9181 gaaaaacaag aacccagtgc aagccatatg gaatgatcac cttgccaata gtaatgatga
     9241 cccataccaa caatacctgt acctagtaat aggattaatt gaaaatataa tgcacgaatc
     9301 gtcgtttcaa tggtagttaa tttcatatta accattaaga aaccaatcaa cactacggca
     9361 aatgcttcaa atataccttc aacccataag tgaacaatcc accatcgcca gtaatctgca
     9421 aacgtcacat gtgaattcgg cattataaat aatgatgcaa gataaaataa cggaattgca
     9481 attgctccaa caaacaatag cgtcaataag tgacgtttat catgattcgc ttgtactttg
     9541 ttgcgaatag ctggtaaaaa ccctcgacaa agaatgataa cccataagac catacctact
     9601 ataaataata tttgccaaaa tttacctaat tctatatatt cccaaccaaa gtgcccgaat
     9661 agccaccaat gagaatcaat ccaacctaaa atatgacccc attctccaat catgctgccc
     9721 atcacaacta tgattaaggc ccaaaacaag atatctacta atacgccttg tttctttggt
     9781 tctttgccca atactttagg tacaatgtat attcctgctg ctaaccaagt cgtagcaacc
     9841 cagaaaataa ctaattgcac atgccatgtc ttcgcaatat taaatggaaa gagcctttgt
     9901 atttctatac caaaaaattt attctctaca taataatgtg ccagtaattc gcctaataat
     9961 atttgaacca aaaatagtaa catgacaacg actaaatatt tcccaacttt tcgctgacta
    10021 tcagtgattt tactatcaac ttcaatcttt ggtagttccc gttccgactc atacgtcggc
    10081 ttcatgtcaa attcataacg cttttgaata tatataatga atgccaagcc tgcaatcagt
    10141 aatgttacag atatagcagt ccataaaatt cctgcactag gcaacgtatt tcctgcatcc
    10201 acatcgtatg gccaattatt tgtatatgaa aatgtttttc caggtctatc tgttgatgat
    10261 aaccaagcac cccagaaaaa gaattgactt aaatgttcaa ctttatttcc tgcgaccata
    10321 taatcatcat tttgaaactg tttaatcata ttttcattta atcctacttg tttagggtta
    10381 ttaacaaact cttttttata ataatcttgc aaatatttta gaccgtatac ttgtcctggc
    10441 gttaatacaa gttgattttc tttttcagaa taacgattaa tccgtatttc tttcatcact
    10501 ttatctttaa ctacggctaa ctctgcatga tttaatttat gaagttgctt atgataaata
    10561 tcttgtgcat aatatttacg cataccaatc aggtaatgat gtaatgcctc tgctgtataa
    10621 tctgggccta agtaggaacc attgcccagg taggagccat aatctgttaa cccatatttc
    10681 tcatatatcg cttgcccact gattagctcg ccttttgtaa caagcgtatg accttgttga
    10741 tctactatct ttgttggacg aggtgcttca tttttaaaaa tataccaacc acctactagt
    10801 agaattgaaa acacaatcac taaaatcgtt agtagcactt taactaaacg tttatatgaa
    10861 ctttgcatac tcatttataa aatctcctct taattgcatt cttacatata agcatacgcc
    10921 gtttttttat aaagtgttga tgacattaag taatttcaag aatttgtcga aaaatattta
    10981 attgaaattt cacaaatgat ataacttaca ttttaattct tagttaaatt aattcattat
    11041 atcatttttc aaaagccgca atacataaaa cgaacgccta aataccataa aacctagtaa
    11101 aaagtaatac atacataaaa acgtctatat cccagttata aaccggagta tagacgtttt
    11161 tttacgataa taacaatggc tcaaattgct attatcttgc ttaggttttt cgttttagaa
    11221 gaatattact acaaagacag gcacaactgc tacaacaact acaccaacta acactaaagc
    11281 tatacttgcc attgattctt ctacaggtcc taattcttta gctggtgcta cacctaatgt
    11341 gtgaccactt gttccaagtg ctaatcctcg ggcaataggg ttagtaattc ggaaaagctt
    11401 caagaattta ttacctaggg cataaataat gacaccattt aaaataactg ctaatgatgt
    11461 taattctttt ataccaccga taccagctga tactggtaac gcaatcgctg tagttgctgc
    11521 ttgaggtaac attgataaaa taacatcatt ggcaaattgt gctaacttcg caaaagttaa
    11581 aataattaat aacgctacaa ctgtaccgat accaatacct ccgatgatac gatgccaatg
    11641 tttaacaagc acttcacgct ttttatataa cggaatcgca aaacagattg ttgctggttc
    11701 taagaagaag taaataatgt ctccacctat tttgtaagtc ttatacggaa tgcctgttaa
    11761 atagaggaag gccacaccaa ataccatact gacaaatagc ggtgcgaata agaagaaacg
    11821 atttgttttt tcaaataata tggtcgctaa gaaaaatggt ataacggata acagtattcc
    11881 gaagtaaggt gtgtttagtg ctaagtggtt aatcatgagc ttgtgcctcc tctattttga
    11941 tcttttttgt gactttgtca cctttagatc ttgaagtaac tttcataata atttgtgtga
    12001 catagccagt acaaataagt aatagtattg ttgagacgat tattagtcca atgattaaaa
    12061 atggtgcttg gctaatgaca cctaaagagt taacaactga gataccggct ggtacgaaga
    12121 gtaagccaat gttatttgtt agtgtcgttc ctactttttc gacttcgcct aacttaacag
    12181 caccagtaca taataataca aataataata ctaagccgat tactgatgca ggcataggaa
    12241 ttggcataaa tgattcaatt attttcgata caaagagtac taaagcaatt acaatgactt
    12301 ggtgaaaaaa gtgtgctggt tttgatgcgt ctttttgttg tttcacgacc attgcctcct
    12361 acgtttgatt taactaaagt atagatggct cactttgatt tgcgtgaatt ttagtccgaa
    12421 atacaaaata tcataggtaa aatgcataaa aaaaaggatt actgttaaag taatcctatc
    12481 gacgctttaa aatctttcat aaatgaacgt ccaacttgca tcttgacacc atttgtcaat
    12541 attaccatat aagtatagtt aaaccattgt tgtacttctt taatgtgttt cgtgttaata
    12601 atatatgaac gatgaatacg tatgaaataa gcgggattca atcgtttttc ataacgattc
    12661 aatggctctg ttgtttcgta tttatgattc gttgtatgta tggttgtaat accattatgt
    12721 gtgccaatcc caataatatt ttgttgcttt aacatgtgaa ttttatcgtc aatttcaaca
    12781 ggtaaacttt gatcaaaatt cgccgacata tcattcgcaa ttgcatttgc gctattatca
    12841 tctttggctt tagtcgcacg aactttattg actgcttgtt caatacgttt ttgaccaaac
    12901 ggtttcaaaa tatagtctgt cgcatttaat tcaaatgcct gtactgcgta ttggtcatgt
    12961 gcagttgcaa aaataatcgc aggtggctct ttcatctttt gaattttagc tcctaattcg
    13021 attccatttt catccattaa attgacatct aaaaatataa tgtcatattg atttatcagt
    13081 aatgcttcca atgtttcttt tacattttct gcctcattaa tttcttcaaa accaccaatt
    13141 tcatttaata aatatgttaa ttcattacgt gctaatggct catcatctat gattaatgct
    13201 ttcatattta ttcctcctct tgtctttcat aaggaagtac acaccaaaaa gtggtaccgc
    13261 tcgatgtcga ttcaaattgt aatgctgcgg attttccaaa taatcctttt aggcgtaagt
    13321 ttaaattttc taaagcacta ccagttccag actctgattc tacagatgtt tctcccaaca
    13381 aatgcatttt atctttagaa ataccctgac cattatcttg tacaataata cgtacatgtg
    13441 ttgcagtttc tttaatcact gacacgtcaa tatcgttacc ttgctttcga ttcgtaaacg
    13501 catgtttgat ggcattttca actaaaattt gaattaaaaa tggtggtaca agcacatcgc
    13561 gatatttgtc ttcaacatta atattgatat taaatcttcc tggataacgt gcttgttcga
    13621 gtgataagta tgcacgcact tgacttaact ctttatctaa agtaatcgta tgttgcttag
    13681 agccttgtaa attcgctctg aaaaaataac tcaattctaa tagtaactct cgtgcctttt
    13741 cgctatttat tctaactaaa gctgaaatcg tgttaattga attgaagaaa aaatgtggac
    13801 ttacttgtgc ctgtaatgac ttaatctcag catctttcaa taacttactt tgcgtttcgg
    13861 cttcaccaag ttcaatttgg ctactaaaaa tatttgccaa tccttctgca agttgacgtt
    13921 ccacaaaagt taaatcatta gggtttgtaa aatacatctt caatgtaccg acgatagaac
    13981 catgcatctc aagtggtatc acgatagctg ctctaagcgg gcaattcgga tgactacaac
    14041 caatctcttc tttagtatga acttctttca actttcctga tttcaataca tctttagaca
    14101 gacttgttaa tatttcattt gttggtatgt gatgatcact acctgcacct acatgcgata
    14161 agatttcatt tttgcttgta attgctacgg cagatacttt cattaaattt ttaataatca
    14221 tcgcaatttg ctgtgccgat tctctattca atccttcttt aaaatacggc aatgtctggt
    14281 tcatcaattg cagtacatca tgtgtttgaa cagccttcat ttgctcctct tgctttaatg
    14341 ttgaaatgat aatagacata aaaatcgccg taccaacgct attaacaata atcattggta
    14401 gtgcaattaa tgatatgagg tcaaccgcat atgctttgtc gtgggaaaat gttaaaatgc
    14461 tcaacatttg aatcatttcc ataacaattc caatcatggc acttttcgca atactcgggt
    14521 aacgcttgcg tctttgagct tgtaaaccaa aataaccagc aattataccg ataaatatag
    14581 atgagataag ataaacttgt gcatccgccc cacccatata cactctgaaa atacctgaaa
    14641 taacgccaac aaatagacct acaaaaggac caccaactaa tcctgcgaca cctatcgtta
    14701 atacacgtgt gttagctaaa gatacatcat catctaaacg gaagtacaca cttcctgaca
    14761 aactatgttg atgatcgatg acgataccag ttaaattaga cattaaggca aacaaactga
    14821 aaataataca taattgccaa cgtgctttcc atgtacgtcg acgattcatt aagtttttaa
    14881 aatatgcaat attcatcagc acataggcca aaataataat taaacctaca cgctcaagta
    14941 ataacattgt tagcgatagc acgatagcac ctcagtgaat tataattatg tttattttat
    15001 cataatagtg catgtaaaat tcatgaataa attattaaaa aaacacgcta ctatttaatt
    15061 atctatacca ttaaattttc atatcaattt ttatgctaag ttgaccattt ttcttttcaa
    15121 aataatgact atcatgtcac taaatttctg ttaagtatag cgaaaaactg ctgacattac
    15181 tttgccaaca gtttctaatc gcgttattta tgatactttt ttaaataaaa catgattctc
    15241 taaatgaaca tgctcatgtg ttaacacttc aagtgctttt aatctctgat aaacgagtcg
    15301 ccaagtacca catgcctcta tcggtggttc atagcttgat gttaaatcgc tcattttcac
    15361 taacaattgt cccgttgcaa tgtgatcaga tactaaatca tcaatcactg tattaatatc
    15421 gtctactact tcaccttgtt catatttaat tagttttgga aaatcaacat catcttcttt
    15481 ttgcatatgc tctaacatgc catttttaaa tgtatcatat gtttctttta actcgactaa
    15541 atatggatga ttaggtccat gtactttcga taatttcgtc acataaggtg ttaaattttt
    15601 aaattcttct ctaagaggtt catgatatgc tgcttgaata tattgaataa gtgacgaaac
    15661 atttaaaaat ttagggttaa gcgaacctgg tgtattcgtt tgttcaatgt cgttgagacg
    15721 ctgtaataat tcgttcaaat ctacattttt cttttctaag gatgctgctt ctatacttat
    15781 ttgtccacca cagcaaaagt ctatacctac gcttctaaaa atatctgctg ctttcggata
    15841 atctgttact acatctgcta ctatgtcatt tttatttatc attatagata acctcctcat
    15901 ttccagtgta tacaatttaa tgtatttaaa aatgataaac gaggaaatgt cataatcatg
    15961 tcataaacag caaaataaaa tcctcaaatc attgtaaaat agcgttggct cacaatgatt
    16021 tgaggggttt tattatttat tttagcgagt aagtcatatg tgcagtgttt aacttaacaa
    16081 tatttttgaa gtctcgcttc ttaatatctt tactaagtaa gatttccact tcgttttctg
    16141 acgtttggct taacattccc ataatcatgt cgtcttttaa cacgacacta gccatacctt
    16201 cttcaaatac ataaccacga tccagaccag ttaaaatgat tttgttttta ccttttaact
    16261 tcattgagat tgaatccgtt aatgtaatag ctgtaaagta taacgaaatg ttattaaagt
    16321 cttttgtgaa atacggtgtt aatctataaa aatcattatt caaatgtatt aatgcacttg
    16381 tttcagcagt atatgcatat ttattacgtt gatttccaac acgaacctca aatacttcag
    16441 tacctatttg gacttctaag aaaacatccc aaattttttc tttaggtaaa tacggtttaa
    16501 tgtcttctaa catgatgtta aaacgatatt gtttattcgc atttacactt aaaggtacat
    16561 acattgtatg cttcttattt cgtgaatgta acttaagtct gacatcataa tgtccttcgc
    16621 ctatcataaa ttcatttaat gttagctctg taacgatttt atggttatca aatttaaagg
    16681 ctgtcatttt aactttcggt ttagcaatat caataccttc aaagaatggt tcaccttcat
    16741 tgaattgaat atttaatttg ccatctacta tatcaaaatg ataatactgc ccttgacgat
    16801 aactttcttc tacagttcta taattatcaa tatctttggc tctcgcatag tgtaataatg
    16861 ggcgtaattt gcttaacact aaggcatcta cgcgttcagg cactgcttgt ataaaatcac
    16921 ctaacgcatt aatccattgt ggttggtctt ccaacttaac tttaagtgaa aaaccattcg
    16981 tacgtgaaaa actaaaatga cgatttaaaa attctgctaa gatttgatct ttatgtgatt
    17041 cttctaaatc agcgtttaaa atttctaatg taatcaatcg cataacttca taaaagtctt
    17101 ctggtgaaac atatgcacta ctcatatgtt ccccttcacg tttagtagca taataatacg
    17161 ctttatcact taacacactg attcgatttg cattcaaata tgctttcatt gtaaataatt
    17221 gatcttctgc actctttaat tcttctggaa aataaatgtc attatctttt agtaatgctg
    17281 ttctatagat tttagtcggg cttaaagtat agataattct tgaatttaac aaagtaactt
    17341 caggtgctgt ttctttaaac atagattgtg gtacaccacg accattaaca cctttcattt
    17401 taatcaataa gacatctgag tgatgttcat caataaatgc tgctgcatcc tttaaagtct
    17461 ctttgtttat atagtcatcg gagtccacaa ataatacaaa ttcaccttct gcttgtttta
    17521 acgccacatt tctaggttta cctggaccac cagaatttgt ttcgagttga cttactttca
    17581 agttcaattt attacgatac ttttcaacta tttgtaacgt ttgatctgtt gaacagtcat
    17641 caactacaac cacttcaaat tcagttttcg gaaaatcttg tttcgcaagg ctattaagta
    17701 attctgttat atacttttct gaattgtatg ttggaactat tactgaaaat ttcatcatta
    17761 tacctctccc actttgacta ctatataaac ttagctacca aataaatttc tgactaaacg
    17821 ctcacttgat cggccatctt gatatttaaa atgtttatct aagaacggaa taactttttc
    17881 cccttcataa tcttcattat ccaaggcgtc cattaatgcg tcaaatgatt gcacaatttt
    17941 acctggaaca aatgattcat atggctcata aaaatcacgc gtcgtaatat aatcttctaa
    18001 atcaaatgca tagaaaatca ttggcttttt aaatactgca tattcatata ttaaagatga
    18061 atagtcacta attaataaat ctgttatgaa cagtatatca ttaacttctc taaagtcaga
    18121 aacgtcaaca aaatattgtt tatgtttgtc tgcaatatta agtctatttt tcacaaatgg
    18181 atgcatttta aataatacaa ccgcgttatt tttttcgcaa tatcttgcta aacgttcgaa
    18241 atcaattttg aaaaatgggt aatgtgctgt accatgacca ctacctctaa atgttggtgc
    18301 gaaaagaatg actttcttac ctttaataat tggtaattca tcttccatct cttgtttgat
    18361 ctgtgtcgca taagcttcat caaatagtac atcagtacgt ggaacacctg taggcactac
    18421 atttttctct ttaataccaa atgcttcagc gtagaatgga atatcggttt cagatgatac
    18481 ataagctttt gtatagctac gatgatttaa tgaatcaata aatggtccac cctttttacc
    18541 agtacgacta aagccaactg ttttaaaagc accaacggca tgccatactt gaataacttc
    18601 ttgagaacgt ctaaaacgca ctgtataaat caatgggtga aagtcatcaa caaagatgta
    18661 gtctgccttc ccaagtaaat atggcaatct aaacttgtcg atgatgccac gtctatctgt
    18721 aatattcgct ttaaaaacag tgtgaatatc atacttttta tctaaatttt gacgtaacat
    18781 ttcattatag atgtattcaa agtttccaga catcgttggt ctagagtctg atgtaaacaa
    18841 caccgtattc ccttttttca agtggaaaaa tttcgtcgta ttaaatatcg ctttaaaaat
    18901 aaattgtctt gtattaaatg attgtttgcg gaaatactta cgcaattctt tatatttacg
    18961 aacgatataa atacttttaa cttctggagt cgttacaaca acatcaagga caaattcatt
    19021 aacatcgcta gaaatttcag gtgtaacagt ataaaccgtt ttctttgaaa tgccgccttt
    19081 tctaaattct tttaggtaag tctgcaataa gaaattgatt ttaccatttt gtgtttctaa
    19141 ttcgttgtat tcttcttctt gttctggctt tagattttga tatgcatcat taatcacatc
    19201 tgggtttaac tgtgcaatat aatcaagttc ttgctcattc actaataagt acttatcttc
    19261 aggtaagtaa taaccattat ctaagatagc tacattgaaa cgacaaacga attgattccc
    19321 atctattttg acatcattcg ccttcattgt acgtgtctca gttaaatttc ttaatacaaa
    19381 attactatct tctaaatcta ggttttcact atgtccttca acgaataact gaacacgttc
    19441 ccaatagatt ttatctatat atatcttact tttaaccaac gttaattcat ccttttctat
    19501 ttacataatc cattttaata ctgttttacc ccaagatgta gacaggtctg cttcaaaagc
    19561 ttctgtaaga tcattaattg ttgcaatttc aaattcttga ccttttaaca acgctaattt
    19621 atctacaata tctgggtatt gaatgtataa gtctacaaca tcttggaaat cttttgaacc
    19681 acttcgacta ctaccaatca acgttagtcc tttttccaat actagacgtg tattaacttc
    19741 tactgggaac tcacttacac ctaacagtgc aatgcttcct tctggtgaaa tgtaatcgat
    19801 catttgattt atggctgatt gactaccacg cccaccaacg cactcaaatg catgatcaaa
    19861 tgttaagcct tcaggtattt tattaataaa gaagacatca tcaacaaatg agaagtgact
    19921 caatttataa tctgttttac caaagacata tattttagac tctggatata atttacgtaa
    19981 taaaatggct gtaatgtaac ctaagttacc atcaccccaa ataccaaatg tatttttatt
    20041 tgaaatagat ttcttttcaa aacgacgaat agcatgcaaa cttactgtta caagctctgt
    20101 atatgaaata atacttaaat caatatcatc aggtagtggt acagctctat catgatttag
    20161 caacacaaaa tcttgcataa acccatcatg tccacttgat ctgaagtagc tcgattttaa
    20221 atagttttca gcaatgacat cgtctttttc tgtcggcgta ttcggtacca taactacttt
    20281 tgtaccttta ttaaacacac ctttactgtc aaatacgacc tcaccaacac cttcatgaat
    20341 taaagacatt ggcaatttct gagataagac attctcatca cggctaccag tataatatct
    20401 ttgatcggca gcacaaattg acatatataa aggacgtaca atgacatagt cactgtaaat
    20461 atctacgttg ttatacgtaa cttcaaattg tctaggtgca actaattgat atacttgatt
    20521 aatcatcggc aataccacct cgaataatag cattcgctac ttttaaatcg taaggtgttg
    20581 ttactttaat gttatataac tcacctttta caagtcggac cggtttgttt gtttctacaa
    20641 taatcttaca agcatcagat aaaatactct tttgctcatc actcaactgt gcatagcttt
    20701 cttttaataa attaatatta aacgattgag gtgtttgacc ttggtacatt tcattacgca
    20761 ctggaattgc atcaatcgtt tgattatctt tagatgtaac aatcgtatct atagcatcaa
    20821 tcactgtatc tactgcaccg tactctaaag cagcttgaat attttcttta ataatgcgat
    20881 gcgttaaaaa tggtctaact gcatcatgtg tcacaatgac atcgtcatcg ttaataccat
    20941 ttgttgattc aatatgttta acgatattca taattgtatc attacgatcg ctaccacctt
    21001 gaatgacttc aattctttca tcagaaattt tgaattttct aagtgtatct ttcgtatgcg
    21061 tcatccattg ttgtggcgtc gcgataataa ttttttcaaa atcattaatt aaaataaatt
    21121 tttctaatgt atggattaaa atcggtttgt tatctaaatc taaaaattgt ttaggtaaag
    21181 gtacgttacc cattcttgag cctatacctc cagctagaat accagcgtat ttcataaaat
    21241 acttcctcca ttcaactata tctatattta attatttaaa attcattgcc tattttttgt
    21301 gataactcgt ttttaaaatc aaaattttaa gcgtcaatgt attacttaaa attacacaca
    21361 ttttgcttat attttagcat attttgttta aacctatatt acattatatc agacgttttc
    21421 atacacaaat aataacatac aagcaaacat ttcgtttatt atttatatca ctaaatcaat
    21481 taatttataa tgttttattg tttttttacg gaatcactta aaaagtgttt ggcaaattcg
    21541 ctgcgatgca tatccatctt ctaatgaaca atatttttga taaaaagctg ttcgtgctgc
    21601 aacataatca ttagctgtta tatctatatg ttttaatgtc tctataagtg ctgcttgatt
    21661 ttcaactata ggaccaggca actctttctt gtaatccata taaaatcctc taagttcatc
    21721 gccatatttt tctaaatcat aagcatagaa aatttgtggg cgctttaaaa caccatagtc
    21781 aaacataaca gatgaatagt ctgttattag tacgtcacta atcaaataca aatcagaaat
    21841 atcttcataa tccgagacat ctttcacgaa atcatcatgc tcgtctacac gagtcacaac
    21901 taaataatgc atgcgtaata aaatgacata attatcatcc agcgcttgac gcaaagcttc
    21961 tatatcaaag ttaacattaa attgatatga accttctcga atcgcttcat cgtcacgcca
    22021 agttggcgcg tacataatca cttttttatc taatggaata tttaatcttg tcttaatacc
    22081 attaatatat tcagtatcat tgcgtttatg tgataattta tcatttcttg gataacctgt
    22141 ttccaaaatc ttatctcgac taacatgaaa tgcatgttga aatatcgatg tcgaatatgg
    22201 attaggtgac actagataat cccaacgttg gctttctttt ttaaagccat cttggtaatt
    22261 ttgagtattt gttcctagca ttttaacgtt actaatatcc aaaccaatct ttttcaatgg
    22321 cgtgccatgc catgtttgta agtacgtcgt tcgtggtgat ttatataacc aatctggtgt
    22381 acgtgtgtta atcatccatg ctttcgctct tggcatcgct aaaaaccatt tcattgaaaa
    22441 ctttgtaaca tatggtacat tgtgctgttg gaatatgtgt tcatatcctt ttttcacacc
    22501 ccatattaat tgggcatcac tatgttcagt taagtattca tataatgctt tggggttgtc
    22561 gctgtattgt ttaccatgaa agctttcaaa ataaattaga ttcttgtttg gcaattttga
    22621 tagtaattta aaagttgtat atatactgtg ttctatcaat tttttaattg tagttttaat
    22681 catgtcgtac ctccgacgtg tttttgtaat tatattaata tatatgagca agctcattgt
    22741 aaccatgcct attatagcat ttcatcataa aatacattta accattacac ttgtcgttaa
    22801 ttatcatacg aaatacatga ttaatgtacc actttaacat aacaaaaaat cgttatccat
    22861 tcataacgta tgtgtttaca catttatgaa ttagataacg attggattga ttattttact
    22921 ttacaaaatg acaattcagt tggaacaggc tgggacataa atcaatgttc tatgctctac
    22981 gaagttatat tggcagtagt tgactgaacg aaaatgcgct tgtaacaagc ttttttcaat
    23041 tctagtcaac cttgccggcg gggccccaac aaagagaaat tggattccca atttctacag
    23101 acaatgcaag ttggggtggg acgacgaaat aaattttgcg aaaatatcat ttctgtccca
    23161 ctcccttatg catgaaaaat caaaaagtta ttctcattgt gtagtcctgc ttctcatcat
    23221 gacatattgc tcacttcatt gtcagaaccc ttcttgaaaa ctatgcctta tgactcattt
    23281 gcatggcaag taatatatgc caacattagc gtctaaacaa atctttgact aaacgttcac
    23341 ttgagcgacc atcttgatat ttaaaatgtt tatctaagaa tggcacaact ttttcaacct
    23401 cataatcttc attgtccaaa gcatccatta atgcatcaaa ggattgtaca attttacctg
    23461 gaacaaatga ttcatatggt tcatagaaat cacgcgtcgt aatgtaatct tctaagtcaa
    23521 atgcatagaa aatcatcggc tttttaaata ctgcatattc atatattaaa gatgaataat
    23581 cactaatcaa caagtctgta acaaagagaa tatcgttaac ttcacgatga tctgacacat
    23641 cgataaagta ttgtctatgt tcacgtgaaa tattaagtct attttttacg aacggatgca
    23701 ttttgaataa cacaactgca ttatgcttct cgcagtatct tgctaaacgt tcaaaatcaa
    23761 ttttaaaaaa tggataatgt gccgtaccgt gaccattacc tctaaatgtc ggtgcgaata
    23821 gaataacttt cttacctttt ataattggca attcatcttc catctcttgt ttaatttgtg
    23881 ttgcataagt ttcatcaaat agtacatcag tacgtggtac acctgttggt acaacatttt
    23941 cttctctaat tccaaatgct tcagcataaa atggaatatc ggtttctgat gatacatatg
    24001 ctttcgtgta actacgatgg tttaatgaat cgataaacgg accacctttt ttacctgtac
    24061 gactaaagcc aactgtttta aaggcaccaa cagcatgcca cacttgaata atttcttgtg
    24121 atggtctaaa gcgaaccgta taaattaatg gatggaaatc atcaacaaaa atataatctg
    24181 ccttaccaag taaatatggc aatctaaact tgtctctcca tttacgtcta tctgtaatat
    24241 tcgccttaaa taccgtttta atatcataat caaaatctac tttttggcga agtaactcat
    24301 catatacata cttaaaattc cctgataaat tcggacgcga atctgatgtg aataatattg
    24361 ttttgcttct tttaatatgt agtaactttg taatattaaa aatagcttta aataagaaac
    24421 ttctactttc aaatgaagct ttatgacctt gtttatgaag ccagtgtgcg cttgtcgcaa
    24481 tgacccctga tttctcttga ggtaaggtga tttcaatatc aaatacaaat tcgttaacgt
    24541 cacttgctat ttccggagta atggtataga ccgtatgttg tgatacgcca cctttacgga
    24601 aaactttagc ttcgttggct aacacatagt ttgcttttcc attttgtgta gtcagctcat
    24661 tgtattcttc aatttgctct tcatttaatg ccgcataagc ttgatcaatg atatttggat
    24721 taagttgtcc aatatactct aactggtcat gataaacaaa taaatattta tccattggta
    24781 aataataccc atcgtctaat attgcgacat tgaatctaca tagaaatgta ttaccgtcta
    24841 ttttcatttc atttgcttct aatgtttttg tttcggttaa attcctcaga acaaattgtc
    24901 ctgatgttaa atctacacct tcactatgtc cttcaatata taactgaaca cgttcccagt
    24961 atatgttatc aatatgtatt gcttgtttcg tttttgtcat tgttattccc ccatcggttt
    25021 attaaatcgt ccatttcaat actgtttttc cccaagatgt cgataaatcc atttcaaacg
    25081 cttggacgat atcttgcatc gtacatacat taatttcatg tccttttaat aaggctaact
    25141 tttcaacaat gtctgggtac ttacgatata aatcaacaac ttgctcaaaa tctttagagc
    25201 cgcttcgact actaccaatc aacgttaatc ctttttcaag tactaatcgt gtattcactt
    25261 ccacgggtaa ttcacttacg cctaacaaag caatactgcc ttctggtgaa atatgttcaa
    25321 ctatttgttg aagtgcaact tgacttcctt tacctccaac acattcaaat gcatgatcaa
    25381 ttttaagatc atctggtatt tgatttactg taaagatgtc atctacaaat gaaaaatgac
    25441 ttaatttata gtctgtctta ccaaatacat aaattttagc ttctgggtac aacttacgta
    25501 gcaaaatagc tgtaatataa cctaagttac catcacccca aataccaaaa ctggttttca
    25561 aaggtataga tttacgttca aatcgttgta tagcatgata acttactgac actaactctg
    25621 tgtatgaaat cgtactcaaa tcaatgtcat taggcagcgg aacgatacga tcatgtgcca
    25681 tcacaacgta gtcttgcata aaaccatcat aaccactaga tctaaaataa ctagaggcta
    25741 agtaattctc cgcaataata tgatgttgct ctgtaggtgt attcggtacc attactactt
    25801 tcgtaccttt ttcaaatacc cctttactat caaatacaac ttcaccaaca gcttcatgaa
    25861 ctaatgacat tggtaatttt ttgcgcagta cattttcatc tcttcgacct gtgtaatacc
    25921 tttgatcagc tgcacaaata gacaagtata aaggccttac gatgacatga ttaccataaa
    25981 tatcaacatt attatatgtg acgtcgaact gtctcggtgc aacgagttga tatacttgat
    26041 taatcatcgg caatatcacc ttgaataatg gcatttgcta tttttaaatc atacggtgtt
    26101 gtcactttaa tgttgtatag ttctccacgt accaatttaa ctgcatgtcc agattcgaca
    26161 atgattttac atgcatctga taagatttct ttttgttcac tacttaaggc gcgataacta
    26221 tcttgtaata atttaatatt aaatgattgt ggtgtttggc cttgatacat ttcattcctt
    26281 acagggatac tgtgtatgtt ctgtttatct ttagacatta caatcgtatc aattgcttca
    26341 atgactgtat ctactgcacc atattttgct gctacttcaa tgttctcttt aataatacgt
    26401 tgagttaaaa atggtcttac ggcatcatga gttacaatca catcatcatt attaattcca
    26461 tttacattgc gaatatggtc gataatgttc ataattgttt cgtttcgatc cgtaccacct
    26521 gcaactactt tgacacgttg atctgtaata ttatattttt ttaaaatatc ctgtgtatgg
    26581 gaaatccact gtgctggcgt tgcgataata atctcattaa agtcactcac taaaataaat
    26641 ttctcaattg tatggattaa aatcggtttg ttatcaatat ccaaaaattg ttttggcaat
    26701 ggcacgttcc ccattctcga accaatacct cctgctaaaa tacctgcata aatcatgttg
    26761 tcctccattc tgtcattaca tcatttccat ttatacatta ctgacctatg cctgcacata
    26821 agcctaacct attgctcact tgcctctttt attaagccaa aggtagttgt cacaatagtg
    26881 tgataatttt tataaaaatg tatttttgta actgaccatt ctaagttgtt ttgccatgca
    26941 gttaatcatt aacactgacg atattaaatt gttaaaggta ttaatgttta ctctttttca
    27001 aattcattat tactgccatc attttaccat atattataat aaatttatct tattaagtgg
    27061 ctgtacttga ttttcacttt aaaaattatc aaatatagcc atctcatttt aagtatacaa
    27121 aatgcaaaac aaccgattca caagcatatt tcacacaagt aaaccggcta tttagcaacg
    27181 tatattcgag gatgaattat ttcgatagta tctgtagacc agccggcatt cgcactttca
    27241 tagctataac tataccagcg ttttcgtcct caaaggtgca tactaataaa tcgtaaacat
    27301 gactttatca aatcgttctt tcttgttaac taatttatca aatgtctccg ggcctttttc
    27361 taacggtaaa aaatgagaaa taataggctt tacattaata tctttcgtct tcatataatg
    27421 taaggttgcc gtccactctt tccccggaaa attactggac aaacagttcc aagagccaca
    27481 tactgtcaac tcattacgca gaattttttc aaaatgaact cgatcaatct caatatcatc
    27541 atatggtatt ccgagtaata ccacctcgcc acctttttta ggtatcgtca atatttgacc
    27601 aatcgtaact ttagcacctg atgattctat agctaaatcg atttgattgg cgtaatgatt
    27661 ttcgatgaat ttctcaagat tttcttcttt tgaattgatt gtttgatgtg cgcccaatga
    27721 ttttgcaata tctagtttat gcgcatctat atctatagcg atgatatgtg cagcaccaaa
    27781 tattcgtgcc cattgaatag ctaacaatcc tatactgcca caccccatta ctgcaacagt
    27841 cataccaggt tgtatattcg atttataaaa cccatgtgca acaacggctg atggttcaac
    27901 cattgctgct tcaatgtaat caacattgtc tggaaccttt aaaacatttt gtgctggcaa
    27961 tttgacatat tccgcgaacg atccaggttc atatgagcca atgacgaata acttttcaca
    28021 tcgtgcatat tcacctttta aacaatactc gcattgataa caaggtattg ctgggcaacc
    28081 tgtcactttg tcgcctacat taacatgcgt aacatcactt ccaatggcat ctacaacacc
    28141 tgaaaattca tgaccaaatg gcataccttt aatgtatggt cccatttttt tgtatcgtga
    28201 cgtgtctgaa ccacatatac cagtcgctcg tactttaaca ataatgtcat tcgcactttc
    28261 aatgattggc ttttcattat cctcataccg taaatcttcc acgccatata atttcaaagc
    28321 tttcacttgt aaatcacctc aactttgatt taattcacaa cttttttctt tttaaaaata
    28381 cctgtcgcaa aataacctgc aatgacgatg gaattactta cgagtaaatg ttccatataa
    28441 aaatcagtga tttgtctcaa tggcccaagc ataaaagtta gcattgattc taccatatta
    28501 attctctcct tcattttcat ctaaatcgag cggcttaagt aatgctttaa taccttcgcc
    28561 agattttaaa tgttgatacg cctcgtccca tttcgaaata tcatatattt ttgtcaccaa
    28621 agcttcagca tttactaaac catccgccat aagttgcaat gaaggttccc aatctgctgg
    28681 cttttgactt ctactaccaa caactgttat ttctttttga atcacttttt ccatatcaaa
    28741 tgggatttca gcatccttaa aaatacctat ttgactgtag aaaccttttt tgcgtaaaat
    28801 atccaaacct tgtcgtgctg ctggaactgc acctgaacat tcaacaacaa catctgcacc
    28861 gtaaccgtct gtaattccat tgatatacgt ttttaagtct gtttgttgta aattgactac
    28921 ataatccatg tgcaatgctt ctgctttatc taatctgact ttgtcattgt ccaatccagt
    28981 taccacaaca gttgcgcctt tacttttcaa cacttgtgct acaagtaatc cgattggccc
    29041 aggtcccatt acaactgcta catcgcctga attgacttga accttagaaa cgccatgatg
    29101 tgcacatgct aatggttctg tcatagctgc agactgatac gatacttcat ctggaatatg
    29161 atgcaaactt tcttcacgtg caatgacata attagtaaat gcgccatcaa cttgtgttcc
    29221 aatacctttt cgatggttgc ataaattata gtcttttgat ttacaatatt cacactcatt
    29281 acaaacatag aatgtcgttt cagaagtgac acggtcacca actttaaaat ctttaacgtc
    29341 tgctccaact tcaacgattt caccagaaaa ttcatgacct aatgtcactg gaaaattaac
    29401 tttataatga ccttcataag tatgaatgtc tgtgccacaa attcctgcat aatgtacttt
    29461 aatttttact ttatcatcta gcggtgttgc aacttcttta tcaagaagtt ctaagttgcc
    29521 atgtccttct cttgttttca ctatagcttt caccacaaac acctcgattt ttaattgaat
    29581 agactaaata gtttaaagat aagatagtta acgatattac caccttgatc aatacttgaa
    29641 atttcagatg aaccttttgg catttgtaca ttcgtacctt tcgccatatc tgtgaaaatg
    29701 ggtgctacgt ctgttgcaat atatagtgaa attgcaatca taatcgtacc cacaatgaca
    29761 gaatgaataa tgtttcctct tgctgcacca acaataaacg cgacaacaaa tggtatagtt
    29821 gctaaatcac caaaaggtag tacttggttt cctggtaaaa taacggctaa taaaacagtg
    29881 ataggtacta aaattaatgc tgtcgaaata accgctggat gacctaatgc tacagccgca
    29941 tccaatccaa tataaatttc acgttcgcca aaacgtttat ttagccatgt tcttgcagac
    30001 tctgaaactg gcattaaacc ttccattaag atttttacca ttctaggcat taataccatt
    30061 actgcagcca ttgacattcc taaattaatg atgtctccag gtttgtaacc tgctaacaca
    30121 ccaataccta aacctaaaat taatccgaca aagatagact ctccaaatgc gccaaaacgt
    30181 ttttgaattg tttcaggatc agcatctaac ttattcagac cgggtactct ttgtaacaat
    30241 ttaactaagt aaatacctgg tgcataagaa attgtacttc ctgtagcaat cgatacacct
    30301 ggtaaatcaa agaactcact catcatcggt gctgtccaat ctgctacttt caaacagata
    30361 acttggaaaa taactgctgc taataacgct tgccaaatac tgcctgatac ggcataaacc
    30421 attgctgctg taaacgtata atgccaaaaa ttccaaatat ctacattcat cgtctttgtc
    30481 actttagtta ctaacaatac aacgttaact atgattccga gtggaataat aaatgctgcg
    30541 acagatgatg cccaagcgat agatgatgtt gctggccaac ctacatcaat cacattcaga
    30601 ctgacgccta aatttttaac catcgcttgt gctgctggcc ctaaattttt aactaataaa
    30661 tcgatgacta agaaaatccc tacaaaagcc acacctattg ttaaaccaga cctaaaagcc
    30721 gctccaattt tctgcctaaa gaataggcca agcaagaata tgacaaccgg taaaataaca
    30781 gttgcaccta aatctaaaaa tccccttaca aaatcagtga agtaactcat atttaaaccc
    30841 tccctgttat atatgcattg tcacgatact ttccgattgt tattacatat gtcgttacag
    30901 tcatttcaac gactaccctt gctacattcg actgcaatcc ctttgaatta cagttactgc
    30961 gtttctatgt catcaacaat catttgtcgt gatagtcatt tatatgcaat ttgcatatat
    31021 ttatatgtta ttgacccaca ttacatatca attcagttat ttttgtaact ctgttaagat
    31081 ttgttgtttt gtttcttcaa taccaatacc agttaagaaa ttacgtgcgt tgataactgg
    31141 gaatttatat tctttctttg tcattgcagt tgtaactaat aaatctgcag tgtcttcata
    31201 aggtccaact tctgtaattt tgatttgttt aatatctact ttaatattgt gttcctttgc
    31261 catttcctca attgcattat ttactactgt tgacgttgca atacctgcac cacacgctac
    31321 taatacttgt ttcattttca attcctccaa ttaattttta gttatattcc aaatatccat
    31381 tgataattgt tgctaaagct gcttggtctt tcgttcttag aatctgttct agtttttctt
    31441 cactttgaaa aatctgcatc aactgctgta atagcttaag ttgatcatct accttgtcca
    31501 tagctaacat aaagacaatt ttcacttctg tctgttgatc aagtgttccc atttcaataa
    31561 acggcacttc tttttctaga acagccacac ctatcgttct atggttaata tgttcgacat
    31621 ctgtatgcgg tatagcgacc gaacatagat gcgttggtaa accagtagca aattcttttt
    31681 ctctgtcgat gactgcatct ttaaacgttg attttacaaa accgttttga tacaacacat
    31741 ctgacatttg cgataatact gattctttat cagttgccga caaattgagc attatatttt
    31801 ctttatgcac taattgctgt cccatccatt tcccctcgtt tctttatttg aataattttt
    31861 taaaatctca tttacatcag aatttttgcg actttgtatg atgcgtttaa ttgcgtcatt
    31921 gtcttgcgcc acatctctca attgtagtaa cgctcttaag tgtgtcactt tatcaacagc
    31981 agcaataggt acaataatat ggattgctgt gccatctgac atgtatattg gttcttgtaa
    32041 tatcaacata ctcatcgctg ttttatgtac atgcttttca gagtatgcat gtggaatagc
    32101 aatattttgc atgataacca tatacgaatc atcaaatacc tgttgcatct catgaatata
    32161 tttaatatca ataaaatgat tagcaactaa cacatcactt gctttagcaa tagcttcatc
    32221 aatattttca acatgatgta ttcttttcac gtgccttgct ggtatcaagt cagctaaatc
    32281 taatgtctta ttttgtgtga caatcgatcc attaatggtt gaaattgaat tataattcgc
    32341 aataaaatct tctaaaccat cacgtagttc tgtaatgtca ttaactgtcg ttgtgcgttc
    32401 aattaatgcc attaacttgt ttatttcctt atcaatgtca gccgattcct tattaatgta
    32461 cttcatcaca tctttacgta actttcgttg ctcattttca gataaagcta cttttgtgat
    32521 aaataatttt ttatgtgtta ggacaaacat tggtgaaaag acgatgtcat aatctaatgt
    32581 gtaattttca aatgttctaa gtgaaatcgc atctaagaaa ataatttctg gaaataagtt
    32641 tcgcaactcg tataacatca tttgtgatac tgacgtgcct tgtgtacaca caataatagc
    32701 ttttatcttg ccatcgaagt tttcatcttg acgtctcaaa ctacccccga acaacatggt
    32761 taaatatgct atttcattat caggcaacga ttttccgaaa tattcagtta acgattgaca
    32821 tgattgtttc accatatgaa ataaggattg ataatttcct tgtaaaggat ttattaattc
    32881 atcacgatcc gttaagttat atttaatcct ataaaaagca ggcgttaaat gtaacaagag
    32941 ttgctgtgat aatttctcct tatcttcaat gttaataaaa gtgatttgtt caaaatggtg
    33001 aatcatttga gcgatggcca tcgttaaatt cgatatgtta tctgattcct gcaaatcagt
    33061 ccattgcaca ctagttgata acaggtgcaa tgttaaatat agtttttccg cttctggcaa
    33121 atccggctca tgttgcgtca taatctccgt tgcttgatat tctttcgtat ccctcaaata
    33181 ctgataatta atatttaatg gattaatgac atgcccactt tgaattcttc tacgaatgac
    33241 acatagaaca taaggcaatg aactaagtga tttgtctata aagcgactct tcaaaaattg
    33301 ttctacctgt ttgattttgt ctttttgata tgcgatatct tcgaatgtta agttgagcgc
    33361 ctttaaaact tcgcttttag taatatcatg attcaacctt tgatcaatca acttaatgaa
    33421 gaaacggcga acttcaaatt catcaccaac aatttcataa ccatgttttc gagaatactt
    33481 aagtgacaaa ccatgatttc ccaattgctc tttcacatga tttatatcgt gaatgacagt
    33541 atttttactg acttgtaaat caattgaaaa atggtttaga gacattgcgt tttccttact
    33601 aaaaagcatg agcattaaat aataacgacg tgtttctatg ctaaaaatga cattgttgcc
    33661 gtttaacatt tgctgctccg atacatctcg cttgaataac gtcatgattt cagaacttac
    33721 aataaaattt ccttggcttg ttctttcaag ttttggataa ccctcttgtt caagccacaa
    33781 attgattttt tgaatgcgat atccaagttg tctacgagac aaaccaaata tcgattcaag
    33841 ttctttacca tgaatagtag gattcaatac aatttctctt agtatttgct gattatcccc
    33901 gttcatgttc gatcctcctt tgagcatctg agctcaattt accatcaatc gtcacaccga
    33961 tgtacacact ttgaacacat attttcaaaa tgagcatgta catcattgtg atgttttaac
    34021 aacattt
//
LOCUS       DAHHRR010000017        33094 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.017, whole genome shotgun sequence.
ACCESSION   DAHHRR010000017 DAHHRR010000000
VERSION     DAHHRR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 33094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33094
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.017"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..278)
                     /gene="deoC"
                     /locus_tag="NBI42_000668"
     CDS             complement(<1..278)
                     /gene="deoC"
                     /locus_tag="NBI42_000668"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCD1777109.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIHNGADEIDMVI"
     gene            592..1302
                     /gene="deoD"
                     /locus_tag="NBI42_000669"
     CDS             592..1302
                     /gene="deoD"
                     /locus_tag="NBI42_000669"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD1777110.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1423..1866)
                     /locus_tag="NBI42_000670"
     CDS             complement(1423..1866)
                     /locus_tag="NBI42_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCD1777111.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLEKAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(2066..2479)
                     /locus_tag="NBI42_000671"
     CDS             complement(2066..2479)
                     /locus_tag="NBI42_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HCD1777112.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFE
                     QHPEAANKNSVILQKGDRIYFESQAIIQLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            complement(2945..4318)
                     /locus_tag="NBI42_000672"
     CDS             complement(2945..4318)
                     /locus_tag="NBI42_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HCD1777113.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRMSNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPN
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSDQFLKYTR
                     IVKVKHD"
     gene            complement(4992..5222)
                     /locus_tag="NBI42_000673"
     CDS             complement(4992..5222)
                     /locus_tag="NBI42_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777114.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            complement(5253..6191)
                     /locus_tag="NBI42_000674"
     CDS             complement(5253..6191)
                     /locus_tag="NBI42_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HCD1777115.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            complement(6628..7293)
                     /locus_tag="NBI42_000675"
     CDS             complement(6628..7293)
                     /locus_tag="NBI42_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1777116.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVAFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGEHDIKDALTQFEI
                     EKD"
     gene            7445..7765
                     /gene="czrA"
                     /locus_tag="NBI42_000676"
     CDS             7445..7765
                     /gene="czrA"
                     /locus_tag="NBI42_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HCD1777117.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            7767..8747
                     /gene="czrB"
                     /locus_tag="NBI42_000677"
     CDS             7767..8747
                     /gene="czrB"
                     /locus_tag="NBI42_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HCD1777118.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            9013..10104
                     /locus_tag="NBI42_000678"
     CDS             9013..10104
                     /locus_tag="NBI42_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1777119.1"
                     /translation="MFTYFKSALKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     VQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLVGIAITTQSIIIIVVLLIKAIITWLLLIPVFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(11015..11119)
                     /locus_tag="NBI42_000679"
     CDS             complement(11015..11119)
                     /locus_tag="NBI42_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777120.1"
                     /translation="MLLLERISMSDFEILIVVLTIIGLVLISNQDHKK"
     gene            complement(11165..11383)
                     /locus_tag="NBI42_000680"
                     /pseudo
     CDS             complement(11165..11383)
                     /locus_tag="NBI42_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            11963..12055
                     /locus_tag="NBI42_000681"
     CDS             11963..12055
                     /locus_tag="NBI42_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erm Leader peptide"
                     /protein_id="HCD1777121.1"
                     /translation="MTYGSIVAILCALFMIFFIPYMEKKDKKKK"
     gene            complement(12184..13041)
                     /locus_tag="NBI42_000682"
     CDS             complement(12184..13041)
                     /locus_tag="NBI42_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1777122.1"
                     /translation="MDNVKAIFLDMDGTILHENNKASTYTKDVINQLREKGFKVFLAT
                     GRSHSEIHQLVPQDFVVDGIISSNGTIGEVDGHTIFKHGLSLTQVKQIVDLAKQQHIY
                     YEVFPFEGNRVALKEDETWMRDMIHSQDPINGVSHSEWSSRQDALAGKIEWVSEFPEG
                     EYSKIYLFSSNLEKITTFRDELKQNHVQLHISVSNSSRFNAETMAYQTDKGTGIKEMI
                     EHFGIRQDETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTTYTNEEDGAAK
                     YLAEHFLAE"
     gene            complement(13108..13890)
                     /locus_tag="NBI42_000683"
     CDS             complement(13108..13890)
                     /locus_tag="NBI42_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1777123.1"
                     /translation="MLITLNHVGRLKQGKPILQDVSWQIAKGDKWVLYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKLGYSAENVRQQIGFVSHSLLEKFQEGETVIDVVM
                     SGAFKSIGLYKNVDDDIISEAHQLINLVEMSDKAQQFIGYLSTGEKQRVMIARALMGQ
                     PRILILDEPAAGLDFIARESLLNILDSLSDTYPTLAMIYVTHFIEEITANFSKILLLK
                     DGQSIQQGAVEDILTSENMSHFFRKKVAVQRWNQRFSMMMLE"
     gene            complement(14117..15922)
                     /gene="glmS"
                     /locus_tag="NBI42_000684"
     CDS             complement(14117..15922)
                     /gene="glmS"
                     /locus_tag="NBI42_000684"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCD1777124.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     DGTPVVALATQENVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            16603..18141
                     /locus_tag="NBI42_000685"
     CDS             16603..18141
                     /locus_tag="NBI42_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCD1777125.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWFPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPIM
                     KFIMHILSVAVESLVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFFGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTKEPKQDLEAATAQMETTKGKKSSVASKLVSSDKDAKVEENASGNVSETS
                     SLDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            18176..20308
                     /locus_tag="NBI42_000686"
     CDS             18176..20308
                     /locus_tag="NBI42_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD1777126.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGADSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSSNNQCEENKIKAFVKKVEMISGMIFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDIDGNTSFENEQTSYLTSVFEKNLSDEKSEQLMHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNR
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIPDNDFMASLVSDLSEFLSLQLES
                     IDTFMEKPQELETLLRNKFLERIKNQFI"
     gene            20320..20754
                     /locus_tag="NBI42_000687"
     CDS             20320..20754
                     /locus_tag="NBI42_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1777127.1"
                     /translation="MSGLFSNDNIFLNVNVSSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEAEA"
     gene            20754..21860
                     /locus_tag="NBI42_000688"
     CDS             20754..21860
                     /locus_tag="NBI42_000688"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCD1777128.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEQIINALATEH
                     QYDVILADESKTTTRVNNVEAINSMQPSEELKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKQAVLDITGPLSDNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVIEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     DKATVLDAIEDSSIEAGLRRVLSETSKYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLP"
     gene            complement(22179..29615)
                     /locus_tag="NBI42_000689"
     CDS             complement(22179..29615)
                     /locus_tag="NBI42_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HCD1777129.1"
                     /translation="MNLFRQQKFSIRKFNVGIFSALIATVTFISTNPTTASAAEQNQP
                     ALNQPAQPADANTQPNANAGAQANPAAQPAAPANQGQPAAQPANQGGQANPAGGAAQP
                     NTQPAGQGNQADPNNAAQAQPGNQAAPANQAGQGNNQATPNNNATPANQTQPANAPAA
                     VQPAAPVAANVQTQDPNASNTGEGSINTTLTFDDPAISTDENRQDPTVTVTDKVNGYS
                     LINNGKIGFVNSELRRSDMFDKNNPQNYQAKGNVAALGRVNANDSTDHGNFNGISKTV
                     NVKPDSELIINFTTMQTNSKQGATNLVIKDAKKNTELATVNVAKTGTAHLFKVPTDAD
                     RLDLQFIPDNTAVADASRITTNKDGYKYYSFIDNVGLFSGSHLYVKNRDLAPKATNNK
                     EYTINTEIGNNGNFGASLKADQFKYEVTLPQGVTYVNDSLTTTFPNGNEDSTVLKNMT
                     VNYDQNANKVTFTSQGVTTARGTHTKEVLFPDKSLKLSYKVNVTNIDTPKNIDFNEKL
                     TYRTASDVVINNAQPEVTLTADPFSVAVEMNKDALQQQVNSQVDNSHYTTASIAEYNK
                     LKQQADTILNEDANHVETANRASQADIDGLVNKLQAALIDNQAAIAELDAKAQEKVTA
                     AQQSKKVTQDEVAALVTKINNDKNNAIAEINKQTTAQGVTTEKDNGIAVLEQDVITPT
                     VKPQAKQDIIQAVTTRKQQIKKSNASLQDEKDVANDKIGKIETKAIKDIDAATTNAQV
                     EVIKTKAINDINQTAPSTSAKAAALEEFDEVVQAQIDQAPLNPDTTNEEVAEAIERIN
                     AAKVSGVKAIEATTTAQDLERVKNEEIFKIENITDSTQTKMGAYKEVKQAATARKTQN
                     ATVSNATDEEVAEADAAVDAAQKEGLHDIQVVKSQQEVAETKTKVLDKINAIQTQARV
                     KPAADAAVENAYNTRKQEIQNSNASTTEEKEAAYAELDAKKQEARTNIDAANSNSDVA
                     TAKDNAIAAINQVQAATTKKADAKAEIAQKASERKTAIEAMNASTTEEQQAAKDKVDQ
                     AVVTANADIDNAAANTDVDNAKTTNEATIAAITPDANVKPTAKQAIADKVQAQETAID
                     ANNGATTEEKTAAKQQVQTEKTTADAAIDGAHSNAEVEAAKNAEIAKIEAIQPATTTK
                     DDAKQAIATKANERKTAIAQTQDITAEEIAAANADVDNAATQANSNIEAANSQNDVDQ
                     AKTNGETSIDQVTPTVNKKATARNEITTVLNNKLQEIQATPDATDEEKQAAETEANTE
                     NAKANQVITAATTNAEVDEAKTNAETAINAVTPKVMKKQAAKDEIDQLQAAQTAVINN
                     DQNATNEEKEAAIQQLATAVTDAKNNITAATDDNGVDQAKDAGKNSIQSTQPATAVKS
                     NAKNDVDQAVTAQNQAIDNTTGATTEEKNAAKDLVSKAKEKAYQDILNAQTTNDVTQI
                     KDQAVADIQGITADTTIKDVAKDELATKANDQKAQIAQAADATTEEKEQANQQVDAQL
                     TQGNQNIENAQSIDDVNTAKDNAIQAIDPIQAATDVKTNARAELLNEMQNKITEILSD
                     NTTTNEEKGKDIEPVRAAYEEGLNNINAANTTGDVSTAKDTAVQKVQQLHANPVKKPA
                     GKTELDQVATDKKTQIEQTPNASQQEINDAKQEVDAALNQAKTNVDQSSTNEYVDNAV
                     KEGKAKINAVKTFSEYKKDALAKIEAAYNSKVNEADNSNASTSSEIAEAKQKLAELKQ
                     TADQNVNQATSKDDIEVQIHNDLDNINDYTIPTGKKESATTDLYAYADQKKNNISADT
                     NATQDEKQQAINQVNQNVQTALESINNGVDNGDVDDALTQGKAAIDAVQVDATVKPKA
                     NQAIDAKAEDTKDSIEHSDQLTSEEKAEALATIKQITDQAKKGITDATTTAEVEKAKA
                     QGLEAFDNIQIDSTHKQQAIEELETALDKIEANVNANTDATIEEKEAFTNTLEDILSK
                     ATEDISDQTTNAEIETVKNNALEKLKGQQINPEAKKNALKEIENAVNKQKETINNADA
                     NKAAKETALNDLSRSHDRFVEDLVKVQSNAEVAELQNVTIPAIEAIVPQNDPDANDTN
                     NGTDNNDATANSNATPENTGQPNVSETTDNGKADASPTTSNNSDAATGETTATSATDD
                     ANDKPQANSNSSVDASTNSPTMDNDVTSKPEVESTNNGTTDKPVTETDNATPAESTIN
                     NNSTTTATNENAPTGSTATAPTTASTEAASSADSKDNASVNDSKQNAEVNNSAESQST
                     NGKVAQPKSENKAKAEKDDRDSTNQSMVESTTETLPSADITEPNVPSNTSKDKEESTS
                     NQTDAGQLKSETNVASNETDKSPSKADTEVSNKPSTSASSEAKDKMTSTNVSQKDDTA
                     TADTNDTQKSVGPVANNKAKDMQTNDMKASLVTVSNGSNSANQDMLNVTKTEENKANA
                     KSAQQGKVNKPKQQTKTLPDTGMSHNDDLPYAELALGAGMAFLIRRFTKKDQQTEE"
     gene            complement(29893..31248)
                     /locus_tag="NBI42_000690"
     CDS             complement(29893..31248)
                     /locus_tag="NBI42_000690"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCD1777130.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIVLDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(31275..32207)
                     /locus_tag="NBI42_000691"
     CDS             complement(31275..32207)
                     /locus_tag="NBI42_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HCD1777131.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(32209..33018)
                     /locus_tag="NBI42_000692"
     CDS             complement(32209..33018)
                     /locus_tag="NBI42_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCD1777132.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
BASE COUNT         9914 a         5850 c         5071 g        12259 t
ORIGIN      
        1 ttgatgacca tgtcaatttc atctgcacca ttgtgaatcg catcttctgt ttcaaatgct
       61 ttcgttgcgg ttgtcgacgc acctaatggg aatcctatta ccgtacaaac tagcacctct
      121 gaatcagcta gtcgctctgc tgcatatttt acatgcgttg gattcacaca tacagattta
      181 aaattgtatg ctttcgcttc atcgatgatt tgatcgattt gcgtacgtgt tgactcaggc
      241 ttcagtaaag tgtgatcaat caattttgca ctattcattt tctatctcct cctttatggt
      301 tgattataaa aatacggttg taaattagtt gattgagcgt caggttcatt taaatatcag
      361 gttagatgtc cacttttatg taaccgcata catacactat tacattaatt catttcccat
      421 aaacaaacaa tacaattgaa catgatatct tcattatgaa cgatgacttg ccaacaagct
      481 aatcaggatt atatttttat aattctttaa ttctatagta caaaaattcg caaaaaaggg
      541 aaacaaatgt tatcttaaaa ttattaatga atattaagga gaagataaca aatgacaaaa
      601 ggtacaccac atattcaacc aaatggagta aaaattgcta aaacagtatt aatgcctggc
      661 gatccgctac gcgcaaaata tattgctgat aattttttag aaaatgttga acaatttaac
      721 gatgtacgta acatgtttgg ttacactggt acatataaag gtaaagaagt ttctgtaatg
      781 ggttctggta tgggtattcc aagtatcggt atttactcat atgagttata caacttcttt
      841 gatgtagata caatcattcg tatcggttct tgtggcgcat tacaagaaaa tgttaactta
      901 tacgatgtta ttattgcaca agctgcatca actaattcaa attatgtaga tcaatacaat
      961 attccaggtc atttcgcgcc tatcgctgac ttcgaattag taactaaagc taaaaatgtc
     1021 gctgaccaaa tcggtgctac tacacacgta ggtaacgtat tatcttctga tacattttac
     1081 aatgccgatc caacattcaa tgatgcttgg aaaaaaatgg gtattttagg tatcgaaatg
     1141 gaatcagctg gtttatattt aaatgcgatt catgctggta aaaaagcact tggtattttc
     1201 acagtaagtg atcatatttt acgtgacgaa gctactacac ctgaagaacg tcaaaattca
     1261 tttacacaaa tgatggaaat cgctttagaa atcgcagagt aacttattta atttgacttt
     1321 aattgctctt taacaatgcg attaaactca aaaagccaac acattctggg cgtatcccca
     1381 tttatgtgtt ggcttttatt tatattatta cttatctgta gattagctta agtaagattt
     1441 aaacatccaa ttatgtttat ctactgatgt ttgcatacct ataaacatat cttctgatac
     1501 atcatcgcca gcattaccag caatttcaat cgctttttct aattgttttg agatattagt
     1561 gaagtcttgt gataattctt caaccatttg ttctgcagag taacctttcg cagcttcttt
     1621 aacaattgat tgctctaagc attcagttaa tgtacctaca gggtttcctc ctaccgctaa
     1681 aattctttca gctaattcgt ctacatattg acttgcttca ttatataatt cttcaaattt
     1741 aacgtgtaat gagaagaagt taggtccttt cacataccag tggaaattat gtagctttgt
     1801 ataagctact gtccagtttg ctacttgttg attcaattct tttacaacat cttgttgatt
     1861 actcatattt aatacactcc ttaaaattgt ctacgtctta cttgtttata ttaattatta
     1921 tattataata attataatct gtaaagtgtt tttactctct tttttataat tattctaatc
     1981 taattgcaaa gtatttcgct tttcaaacgc ctaaatttta atccatcatc tagtatatct
     2041 attatcaatt agcaagtaag ttttatcaat cagtaagttt tactttcgca ttatcatcta
     2101 aaaagaacga tttttcataa tcatttggtt ggtgccatgt tgttttccac attctatttc
     2161 tattattagc aaacattcga taaccgaaat tacggactgg tttaggtaca agccataaac
     2221 ctacgcccaa taatttagtt cgattaggta gtgctgtgat tagttgaata atcgcctgtg
     2281 attcaaaata tatacgatca cctttctgta aaatcacact atttttattt gccgcttctg
     2341 gatgttgttc aaaaaattgt tgtccaactt caccttttaa cgtagcaaat tgataatttc
     2401 ttggtaggtc atgttgaatt aaccaaatga catagttata acaatatata cagtttccat
     2461 cataatatac gattggcata atttattcgc tccttcaata gtacttatag tcatttttcc
     2521 taatttaaaa acatttaaac aaggttgtca cttgataagt cctcgtgaca cgaaattgcc
     2581 ataaaattta tttttcagtt ttatatttat tgtataatgt attttttatt cactttaaat
     2641 ttctcaatct acagtataat attatttctt attatcaact tagcgtttgg tcattcgtct
     2701 tattcaacat accactggaa aactcatata aagttttaat tcagtaacat aaattcatat
     2761 atattcatat actgtcttta ttatttatta aaataaaaac gagtaatcta tcataagctc
     2821 ttaattagct cttatggttg attacccgtc accttttctt tatacggtta ttcaaatatg
     2881 caattatgca attatgcaat ttttaatctt atccttactt cttttcaaaa actaccatat
     2941 atcattagtc atgtttgact ttgactatac gtgtgtattt taaaaactgg tctgaatatt
     3001 tctccttaat ttcctctaaa ctgtcatatg taatacctac aatatgccaa ttaggattga
     3061 aataatagaa agaatctttt tgtcgtgacc atttaatcat attaccgtct ttattataac
     3121 gtggtaatgt tacttcatag ccttctaaca cattaatata cgtttggaaa atgtctgtat
     3181 ctatacgatt aaaattatca ataactaaat aacttttatc ctttttaggc attaattgag
     3241 taataaatcc ttcacgatag tataatgccc ctgtttcatt tggtaaatac ttgccataaa
     3301 gcatatcttc tgaaaaatta ggatgacctg ttgttataac aggatttgca ttcgcttttt
     3361 gcaataagtc attagccgct tttaagcctt cttcttttcg atctacaaca agtaaaataa
     3421 atggctttaa cgcttcttct ttttcacttt caagcatatc tggtttttga accatttcaa
     3481 atggagattt caatccatta ttatcgctca tttcaataat tgcatcatac tgtgcttgtg
     3541 acatacttgc aatagcctgt tttgcatttt cttgaaggaa atataagttt ttcaatttag
     3601 gatgcttatt taatgtactt aatgtaatcg gcgtaatgtc tttctcataa gacacttcaa
     3661 tcactgtact atttgttcta ccaggaattg gtggtttttc atgaatatgc tttgatactt
     3721 ctccaattcc aacgacagat tgatttttcg ttcgattata aaaaataata ttgtcgcctt
     3781 cttctaactg agtataaaaa tgataaccat tacgtttaat accgttgtaa gtgtgcgtat
     3841 aaatcgtata ttggtttcca ggttcaaatt cttcagtttc agctaaaaag aaataacgtg
     3901 gtatcttaat ttcgccttta ccaagaccac ttattaaatc aaactcttct gcagtgattt
     3961 ggttgaacaa tgtctctttc atattactca tacgaaattc caaagcttca ctacgcttta
     4021 aataatctgc tgttaatggt ttcaattgtt cattaaaacg aaactgtaca cgtattttat
     4081 tttgtgcacc tgtttcaaca ctaataattt caccacatcc aagtagtcca gtatccgtct
     4141 gaacttgata aaagatgact tgatctccta ctttagcctt tttaaacgct ctaaatcctt
     4201 gagatgggtt aaaatgtgcg cctgattcaa ataaagctgt ttgtcctact aacggttcat
     4261 tatgattcca acggttatat ccacaattca accaaaaata attcgtttct gctgtcatct
     4321 taatactcct taacctgaat aaattttaga aacactatga attacattct tttagtgttt
     4381 cttatgcagt tgggcgcgta tgcgatcaac tgtatcccct ttgttcactg cgattttaat
     4441 cgcatttcct ataacattgt agcgcctagg acattaattt acgtcccaga cccttatcat
     4501 tttcacttct aagtaagtcg aactattttg ctttacaaca agtgcgactc tatatgcagt
     4561 tggacacaca catacgagca actgtatacc ttttaatcag ttttctatat tttatttatt
     4621 atatctgtct taatgataaa aattgttaca aacagtttaa catatttagc tacctatttt
     4681 gcatataaaa aacatgcttg catacactat gcaataaaaa ttacaaattt atatatgata
     4741 ccactatgct tgcttatctc tatagcacca ttgatacaca tttttaaata tctatactgc
     4801 cgttaaaatt ttatcatgtc ttaattttca ttaaatatta attacttcat tttatataaa
     4861 ctaacaaaaa ccccctcact actatgcaag tgagaggtta tgttgatgtg ctttattttc
     4921 atcaatgtca ttaacacaat catatttctt ctgttaatgc actgtattat attcaaacgc
     4981 gctatcgaca attaatttga agctacaaag gtaccgcata atggcagcac attaattaac
     5041 aatgcgctaa actttaatga ggcgctgatt tgcttgtctt taaaagtcaa catagcactg
     5101 ataattccta aaatagccac tatacgtgga attgtcatac tcggatactg cggtatatgt
     5161 aagaaagcac caagacctgc tatcacacca catactaacg caatatatag aaattgcttc
     5221 aaatcattca ctcctaaatt gttattacac tattacacat agctaatcat caatgtgaaa
     5281 tcaccttcaa agacactatc caaatcttca gaagtcaaaa taaagtttgt accagtagtc
     5341 agtttgaaaa tttcaccatc gacaatcatt tgcccttcac cttccaacac tgtaactaaa
     5401 cagaactctc taggcttcat ataatttaac gtgccagaaa tttcccattt aaccaatgta
     5461 aagaaatcat tcgatacaat gtgtgtacac ttatggtttt caataatttc actttcaggc
     5521 aaaatattag gtaatggtgc attgtactga ataacgtcta aagctttttc aatatttaac
     5581 ggtctatcat tatattgatt atcttgacga ttgaaatcat aaagtctata tgtaatgtct
     5641 gacgattgca tcgtttcgta tgctagtatt cctgaagaaa tagtatgtac tgttcctgct
     5701 ggaataaaat agaattctcc aggttttact ttaatatatc taagtatcga ctctatcgtt
     5761 ccgtgttgaa catgattcgc aacttcttct ctagactctg ctaatgtccc tataactatt
     5821 tctgcatctt cttcagcatc tataatatac caacattcag atttgccata ttgcccattt
     5881 tcatgctcat aagcataaga attatcaggg tgcacatgaa tagaaagtga ttctcttgca
     5941 tccactattt tagttagaag cggaaaatct ttgcttggga aatcaccaaa caattcacga
     6001 tgttctgacc aaatacggtc taatgtttga ccttgatatg gtccattaat aatctcgctc
     6061 gtaccatttg gatgtgctga cacacaccaa cattccccag ttgtatcatt atctaattga
     6121 tatccaaact cacttagacg ttgaccgccc cataattttg tttttaaaat tggttgtaaa
     6181 aataatggca ttgttgcacc tccattgtga ttaagtaagc aatagaactc tgatgttgtt
     6241 gttccattat attttgattt tgttctcatt tatatcgtat tataaacttc cacatttcaa
     6301 attaactatt agtgattgta ccatatttac taacattgca gtactgccaa ttaaagcgct
     6361 tcacttaaat ttacagtact ttaacatttt ttaaaattta tagcatagag attatatctc
     6421 tcttacattt gtacatattt ccctttaaat ttactcgccc attataccaa ttaataaaca
     6481 actttaatag ttgtgccata cattgttcaa attctttgta aaacgcataa aaaatacgtg
     6541 cttattcata cttataattc atcattttca aaaaaaataa cgagttacga aaaagtaacc
     6601 cgcttcaaat catatttact atccttatta atccttttca atttcaaatt gagttaaagc
     6661 atctttaatg tcatgctcac cactaataat ttgaaactct tggtgattaa aatgattgga
     6721 tgtgacaatt tcttttaata ctgtcgcaac atcttctcta ggaatttcac ctttaccgtc
     6781 aaaatattgt gcagcttcta tctttccaga tcctgctgca tttgtaagtg cccctggatg
     6841 taaaattgta taatttaaac ctgaacgtct taaatagtca tcagcgtaat gtttagcaat
     6901 tgtatatggc tttaaatcac cgctatcatc aaaagcctga cgtctcgaat catatgttga
     6961 aaccataaca tagtgtttaa tattagcctc tttactcgca atcattgatt taacagcacc
     7021 atctaaatcg acaataattg ttttatctgc acccgtgttc cctccagaac ctactgaaaa
     7081 gataacttta tcgaatggtt taaacgtctc agttaaagtc tctattgaat cattttcaac
     7141 atcaacgaga attgctttca taccttgtga ttttaacgca ttaagttgat cggattgcct
     7201 aacacctgca gtaaatgcta cattttcttt tgctaattgt tgcactagta acgaacctac
     7261 accgccatta gcacctataa ccaaaatatt catttacaac actctcctat ttattattct
     7321 ctatgccata ccactttatg agatatgtaa aacttgttac aactataaaa atcaattgac
     7381 atactactgg gaacgtatta aattaatata tgaacaaata ttcatatgaa gggattgtca
     7441 tataatgtca gaacaatatt cagaaataaa tacagataca ttagaacgcg taactgaaat
     7501 tttcaaggca ttaggcgatt acaatcgaat acgtatcatg gaattgttat cagtcagtga
     7561 agcaagtgtt ggtcacattt cacatcaatt gaatttatct caatcaaatg tctcgcacca
     7621 attaaaatta cttaaaagtg tgcatcttgt gaaagcaaaa cgacaaggcc aatcaatgat
     7681 ttattcatta gatgacatcc acgtagcaac tatgttaaag caagccatac atcacgcgaa
     7741 tcatcctaaa gaaagtgggt tataatatgt ctcattcaca tcatcatcat gaccatatgc
     7801 atagtcatgt aactacaaat aataagaaag tattgtttat atcgttttta ataatcggtc
     7861 tatatatgtt tatcgaaatc atcggcggtc tccttgctaa cagcttggca ttactatctg
     7921 acggtatcca tatgtttagc gacacattct cattaggtgt tgcacttgtc gcatttattt
     7981 atgctgaaaa gaatgccaca actacaaaaa catttggtta taaacgtttc gaagtactcg
     8041 cagcgttatt taacggtgta acgctttttg taataagtat tttgattgtt tttgaagcga
     8101 ttaaacgttt ctttgttcct tctgaagttc aatcaaaaga aatgttaatc attagcatta
     8161 ttggtttaat tgtcaatatc gttgttgcat tctttatgtt taaaggcggc gacacgtcac
     8221 acaatttaaa tatgcgtggt gcttttctac atgttatcgg agacttatta ggttcagttg
     8281 gcgccattac tgcagctatt ttaatttggg catttggatg gactatcgcc gatcctatcg
     8341 caagtatttt agtttccgtt attattttaa aaagtgcttg gggtatcaca aaatcttcaa
     8401 ttaacatttt aatggaaggc acaccaagtg atgttgatat agatgaagtt ataactacta
     8461 ttaaaaagga ttcacgaata caaagtgtgc atgattgcca tgtttggaca atttcaaatg
     8521 atatgaatgc attaagttgt catgttgttg tagaccatac attgacaatg aaagaatgtg
     8581 aattattatt agaaaacatt gagcatgatt tattacattt aaatattcac catatgacta
     8641 ttcaattaga aacgcctaat cacaaacatg atgaatcgat tatatgttca ggaacacata
     8701 gtcattcaca taaccaccat gctcatcatc acgcacatgt acattaataa ttttaaccta
     8761 ctgccattgc atcgattaaa cttttcaatg gcagtaggtt ttttatatct ttatagcgac
     8821 ttgtttggtc tttgatgatg caatgtttat taacagattt tcaactatta tttcttacat
     8881 tagtcatatt tttgacaatt tactattata attctctaac tttagtcact ttaattaatt
     8941 tttattagat attaatatga aaataacgtg ttttttgtta ttgtatttat atgaagaaaa
     9001 ggaggcacca ctttgtttac ttactttaaa tcagcattaa aaaatgctaa accacaatta
     9061 ttaataacat tgatatatgc acttatagca tttgctgtaa ttgcagttgt atatttactt
     9121 gctaatttcc agcttgctaa gtatgtacaa actattgcaa tttattcaca atttgggcaa
     9181 aaaccaccag tagatgcata tttaaaagtt atcgctgtat tacttatagc agctgtagtt
     9241 tcactgtttg ttttagttca aattttcatc ggaattacaa atgttatgaa acgagcaatg
     9301 agtcatgaaa aagtaaaatt taccgattta tttattgcat ttaaaaaagg taattattta
     9361 aagagcgttc tgatcggcct tgtatctata gcaatgatta tcgttctttc acttctgaca
     9421 tcattactat acaaattatt ttctccagta tcagaaatga ttatgaattc agtacaatca
     9481 tcatatgcag atagtacaca cctggttggt attgctatta caactcaaag catcataatc
     9541 attgttgttt tattgattaa agcaattatt acttggttat tgttaatacc tgttttcaac
     9601 tttatgacta gtttcgttga atcaactaac gataaagtaa aaacacactt agctaatgga
     9661 tttaaagcaa tgaaaaatgg tcaaaaaaca tttttcaaat ttttcattgg aatactatta
     9721 ttaaacttaa ttattatttt atttaaaaca cccgttggtt acttgatttc atttaatacg
     9781 caatcactct ctcaaagtgt tgcagaaaat atcataagag tttatacagt aatgacaatc
     9841 attttattcg ttgttattca tgcaatcatt ttaatgggta tcgttcaata ttacttaaaa
     9901 cgtggtcaaa aaattactaa agataaagta aaaacagctg acaagaataa aaaagttgtc
     9961 actgaaccaa aaaatacaaa agtagaaaat gataaagtga caacatctgt tgaaacgaaa
    10021 acagaaaaag cacaagattc attaaatgat aatacaacaa aaacgatgac ttcagataaa
    10081 ccagaagaca atcaaccaaa ataaatttta gagcccctta caactttagt tgttaggggc
    10141 tcttatgcag ttgatgcgca gcatccaact gtgttcctct attttacaac tttagttgtt
    10201 agggctctta tgcagttgat gcatagcatc caactgtgtt cctctatttt acaactttag
    10261 ttgttagggc tcttatgcag ttgatgcata gcatccaact gtgttcctct attttataac
    10321 tttagttgtt aggggctctt atgcagttga tgcgcagcat ccaactgtgt tcctctattt
    10381 tacaacttta gttgttaggg gctcttatgc agttgatgca tagcatccaa ctgtgttcct
    10441 ctatcttaca actttagttg ttaggggctc ttatgcagtt gatgcgtagc atccaactgt
    10501 gttcctctat tttacaactt tagttgttag gggctcttat gcagttgatg cgtagcatcc
    10561 aactgtgttc ctctatttta caactttagt tgttaggggc tcttatgcag ttgatgcata
    10621 gcattcaact gtgttcctct ctacttcaac tttagtagct aataccgaaa taacatgacg
    10681 ctaatttcat ggcatgctga aaaaatatat gaagaggtct gcagtgcttt ttatttagtc
    10741 cgtgattaca ttctatactt ttaattgtat tttctacttt atggatagtt ggttttatta
    10801 attctgatta tgttcaaaac tacaattcat aagaaaaatg agttaaaata tatttgtagt
    10861 aagtagaagc aaaaggtgaa aatctttatc tcttgaaaca caaaaagggc aacacttgga
    10921 aacatgttag cctaatgagc ccgttaaaaa gacagtgacc ttatatttta tttaaaaata
    10981 gccttcaaaa atgccggtca aagcgaatag aaggttattt tttatggtct tgattactga
    11041 tcaatactaa accaataatt gttaatacaa ctatcagtat ttcaaaatca gacatactaa
    11101 tcctttctag gagtaacaac tataaccata ggcatcaccc ctttattaag agattagcca
    11161 ccatctatcc aacttgctga ataagtatta taaaatgagc atgtttaaac agtaattcat
    11221 aaaataagtt tttcatttct ttaacaactc gataaacagt tgagcgacat acccctgtct
    11281 gactagctat ttcttcgcct gttatctttt gttcatcata taaaaattta atttctcact
    11341 ttttatgctc tggtaaagaa gagcaccaac cttttcggct tataagctcc cttcaaaata
    11401 gacattgaaa aatgaaaact ttgaaagaga aatcgtcagt ctattattga taagtattat
    11461 cctatcatcg aaaaattact ttcagacact agtgaacaaa atttttacta taagcgtata
    11521 ctttggcaat attcgaagga taaacatgga ttaacctgtg catatcatac atttagagct
    11581 tatatcctta aacatgatga atttagtcct tatatttaga tgaaattcat gcttaccaag
    11641 acgaattgta tttggaagag attcctaagt tttttatctt aatgaacgat ttaactacta
    11701 agttcatact agtacaggta aaatgcctat tattgcctaa aagaaagaaa aaattttctt
    11761 acaaccacta cagaatgtaa atgtaaaaat caaatataaa gttaaacata tcttacttat
    11821 ggaatgaaat gtttgatgat tccaaaatca caaatgcgat tttggatttg ttagtgtccc
    11881 ttagcgcaaa aactctagtt aaaacaacgg tagttgttat agtatattta aatgcataaa
    11941 ccatataaaa ggagaaataa ctatgacata cggttcaatt gtagctatac tctgtgccct
    12001 atttatgatt ttcttcattc catatatgga gaagaaagac aaaaagaaga aataacacta
    12061 gtatctggat tactagcata caaaagagca gtaagacatt ttctaataga aaataacttt
    12121 actgctcttt gtacattact catcaactat tgtaaattaa ataataaata actgcctatt
    12181 ttattattca gctaaaaaat gctctgctaa atatttagct gcgccatctt cttcattcgt
    12241 ataagtcgtt acatctgaag ttaatgcttg tatttcagga cgtgcatttt tcatagcaac
    12301 tgtataatga ccaaattcaa acattgccct atcattatca ctgtctccaa taaccaacgt
    12361 ttcatcttga cgaatgccga agtgttcaat catttcttta ataccagtgc ctttatcagt
    12421 ttgataagcc attgtttctg cattaaatct cgatgagtta gaaacactga tatgtagttg
    12481 cacatgattt tgcttcaatt catctctaaa tgtcgtgatt ttttctaaat tcgaactgaa
    12541 aagataaatt tttgaatatt caccctccgg aaattcactt acccattcaa tcttgcctgc
    12601 aagcgcatct tgtctagaag accattcact atgactcaca ccattaattg gatcttggct
    12661 atgaatcatg tctctcatcc atgtttcatc ttcttttaaa gctactctat ttccttcaaa
    12721 tgggaatact tcataataaa tatgttgttg tttagccaaa tcaactattt gctttacctg
    12781 tgttaaagat aatccatgtt taaaaattgt atgtccatct acttcgccaa tagtaccatt
    12841 tgagctaata ataccatcaa caacaaaatc ttgtggtact agttgatgta tttcagaatg
    12901 tgaacggcct gttgctaaaa atactttgaa tcctttttct cgcaactggt taataacatc
    12961 tttggtataa gttgatgcct tattattttc atgtaaaatc gtaccatcca tgtctaaaaa
    13021 tattgctttt acgttgtcca tattatcctc cactatcaat gctatttaat taaattgtgt
    13081 cattattact tattatttgc aaagtattta ctctaacatc atcattgaaa aacgttgatt
    13141 ccatcgttga actgctactt ttttccggaa gaaatgagac atgttttcag aagttaatat
    13201 gtcttctaca gcaccttgtt gaatactttg gccatctttt aacagtaaaa ttttggaaaa
    13261 gttggcagtg atttcttcaa taaagtgcgt aacataaatc atcgcaagtg ttggatacgt
    13321 atctgacaag gaatctaata tattcaatag tgactcacga gcaataaaat ctaatccagc
    13381 tgctggttca tctaaaatta aaatacgcgg ttgccccatc aatgccctag caatcattac
    13441 acgttgtttt tcaccggtgg ataaatatcc tataaattgt tgtgctttat cagacatttc
    13501 tactaaattt attaattgat gtgcttcact tattatgtca tcatctacat ttttatatag
    13561 acctatagat ttaaaagcac cactcattac aacatctatt accgtttcac cctcttgaaa
    13621 tttctccaat aagctatgtg atacaaatcc tatttgttga cgtacatttt ctgcagaata
    13681 acctaattta cctggcattt taccgaacag attaacagtt ccagatgtcg caggctcata
    13741 cgcatttaaa atatttagta gtgttgtctt ccctgctccg ttcaaaccat ataataccca
    13801 tttatcacct ttagcaattt gccaagaaac atcttgtaat ataggcttac cttgtttaag
    13861 acgcccaaca tgattcaatg tgattaacat atgcatccct ccctttttaa ggtaatgttt
    13921 aatactttaa aattatcgta acatataata acgaattgtc agattattat aacaaatcaa
    13981 agtatgtaga atatgatttt tggcataaat caagattacc attttaagat taaacaaaaa
    14041 caccccttta gactcaacat ctaaaagggt gccatatact ttaattttaa tacatgattg
    14101 attctaaaaa agtgaattat tccacagtaa ctgatttagc aaggttacgt ggtttatcaa
    14161 catctaaatc tctgtgtaat gctgcatagt atgaaattaa ttgtaatgca acaactgata
    14221 ctaatggcgt taataattca tgtacatgtg gaatgacata agtgtcgcct tctttttcaa
    14281 gaccttccat agaaatgata catggatgtg caccacgtgc cactacttct ttaacgttac
    14341 cacgaattga taaattaacg ttttcttgag ttgctaaagc aacaactggt gtgccatctt
    14401 cgattaatgc aatcgtacca tgtttaagtt cgccaccagc aaaaccttct gcttgaatgt
    14461 aagaaatttc tttaagtttt aacgcacctt ctaaacttac gttatagtca atagtacgtc
    14521 cgataaagaa tgcattgcgt gttgtttcta agaaatctgt agcaatttgt tccataattg
    14581 gtgcatcgtc aacaatcgct tctattgctg ttgttacttt tgctaattct ctcaataaat
    14641 caatatctgc ttcacgaccg tgctcttttg caacgatttg agacaagatt gataatactg
    14701 caatttgtgc agtatatgct tttgtagatg caactgcgat ttcaggaccc gcgtgtaata
    14761 acaatgtgtg gtctgcttca cgtgataaag ttgaacctgc aacattagtg attgttaatg
    14821 atttatgacc taatttatta gtttcaacta atacggcgcg gctatctgca gtttcacctg
    14881 attgagaaat ataaacgaac aatggttttt cagataataa tggcatgttg tagacaaact
    14941 ctgatgcaac gtgtacttca gttggtacgc cagcccattt ttctaaaaat tctttaccta
    15001 ctaaacctgc atggtagctt gtacctgctg caataacgta aatgcggtct gcttctttaa
    15061 catcattgat gatgtcttga tcaattttca agttaccttc tgcatcttga tattcttgaa
    15121 taatacgacg cattactgct ggttgttcat gaatttcttt taacatgtag tgtgcataaa
    15181 cacctttttc agcatctgat gcatcaattt cagcaatata tgaatcacgt tctacaacgt
    15241 ttccatctgc atctttaata ataacttcat cttttttaac aataacgatt tcatggtcat
    15301 ggatttcttt atattcgctt gtcacttgta acattgcaag tgcgtctgat gcgataacat
    15361 tgaaaccttc accaacacct aataataatg gtgatttatt tttagcaaca tagattgtgt
    15421 ctttgtcttc agcatctaat aaacctaatg catatgaacc atgtaataat gacacaactt
    15481 ttgtaaatgc ttcttcagtt gaaagtcctt gatttgaaaa gtattcaact aattgaacga
    15541 taacttctgt atctgtttct gaaatgaatg atacaccttg taagtattca ccttttaact
    15601 cttcatagtt ttcaataaca ccgttatgaa ctagagtaaa acggccattt gatgattgat
    15661 gtggatgaga gttttcatga ttcggtacac cgtgtgttgc ccaacgtgtg tgaccgattc
    15721 caacaggtcc atcaaaatcg ctactatcag caactttacg taattctgca atacgacctt
    15781 tttctttaaa tacagttgta ttatcatcat ttactactgc gatacctgca gagtcataac
    15841 ctctatattc taatttttct aaacctttta ataataattc tttggcatta tcatagccaa
    15901 tataacctac aattccacac ataacttcat tttcctccat attggaatag tacgcgtaaa
    15961 ttatgattta ttgccgataa tttagattga caatctgctt tcataatata aataggaaca
    16021 tgctatcatc gcattcatcc ataacaaatt aagcatagtt atttttacaa ctatacaaat
    16081 tgctcacact gtactttcca tattaatatt ttttatattc aatttctggc gatcttatta
    16141 actttgtcca ttaagtcacc ctaatgtttt acttaataag ctaacgaatg agccacatcc
    16201 gggatagcat ccgccgatct attcgatcac tatcctcctc gtctacaaat acatatattg
    16261 cactctataa aggccactca tatattaacc tttaatcttc aaatacaaat atttatttgc
    16321 acaggcgctt taactgtact gccgaacttt ccccctttcc attaatcatt attgtacaac
    16381 ggtgttgttt tgttttgcaa atattttcac aataaaattt taaaaatcct aaaacaattt
    16441 ttttgtttta ctttttcaaa atatctatac tgtcacattt atgacacttt atttaatttt
    16501 gtcacattta ttttgacaaa gttgattttt gtttatattg aggattgtgt tatgggtctt
    16561 tacactaaag atagtcacat acattaaaaa agaggtgtaa atatgtcgca aactgaagag
    16621 aaaaaaggaa ttggtcgtcg tgttcaagca tttggatcat tcttaagtag tatgattatg
    16681 ccaaacattg gtgcattcat cgcttggggt ttcatcgcgg ccatttttat agataatggt
    16741 tggttcccta acaaagattt agctacttta gctggtccaa tgattactta tttaatccca
    16801 ctattgattg catttagtgg cggtcgttta atttatgatt tacgtggtgg tattatcgcc
    16861 gcaacggcaa ctatgggggt catcgttgca ttacctgata caccaatgtt acttggcgct
    16921 atgattatgg ggcctttagt tggttggtta atgaaaaaga cagaccaatt aatccaacct
    16981 agaacaccac aaggttttga aatgctattt aataattttt cagctggtat tttaggattt
    17041 atcatgacaa ttgcaggatt taaaatttta gcaccaatta tgaaatttat catgcatatt
    17101 ttatcagtag cagtagaatc attagtgcat gcacatctac ttccacttgt aagtatttta
    17161 gtagaacctg caaaaatcgt atttttaaac aatgcaatta accacggtgt attcacacca
    17221 cttggtgctg accaagccgc aaaagctggt caatctattt tatacacaat tgaatcaaac
    17281 ccaggaccag gtttaggtat tttacttgct tatatgattt ttgggaaagg tactgcaaaa
    17341 gcaacgtcat atggtgctgg aattatccac ttcttcggag gtattcatga aatttatttc
    17401 ccatatgtat taatgcgtcc tttattattt atcgctgtta ttttaggtgg aatgactggt
    17461 gttgcaactt atcaagcaac aggatttggg tttaaaagtc ccgcgtcacc aggttcattt
    17521 atagtttact gtttaaatgc acctagaggt gaattcttgc acatgttact cggtgtcttc
    17581 cttgctgcac ttgtatcatt tgtcgtagca gctttaatta tgaagttcac taaagaacct
    17641 aaacaagatt tagaagccgc aactgctcaa atggaaacta ctaaaggtaa aaaatctagt
    17701 gttgcttcta agttagtatc ttctgataaa gatgcgaaag ttgaagagaa tgctagtggt
    17761 aatgttagtg aaacatcttc attagatgat gatcctgaag cgctattgga taattacaac
    17821 actgaagatg ttgatgcaca caattacaat aatataaatc atgttatttt tgcctgcgat
    17881 gcgggtatgg gttctagtgc aatgggcgca agtatgttac gtaacaaatt caaaaaagca
    17941 ggcattaatg atattactgt aacaaatact gcaattaacc aattgccaaa agatgcacaa
    18001 ttagttatta ctcagaaaaa attaactgac cgtgctatta aacaaacacc aaatgccatc
    18061 catatttcag tggataattt ccttaattca ccaagatatg aagaactttt aaataatcta
    18121 aaaaaagatg atcaagcata ataattaaat aaattaaaaa atggaggata ccgccatgtt
    18181 attgagtaca cgtgaaaaag aaatgatagc cctattgatt aagtaccacg gtcaatatat
    18241 cactatacac gacattgctc agcaacttgc ggtgtcctct cgtactattc accgtgaatt
    18301 aaaaggtgtt gaagcatatt taacttcatt ttcattaact ttagaacgcg caaacaaaaa
    18361 agggctacgc attgctggcg cagattctga tttaaacgat ttgaagcaat caatcgcaca
    18421 acatcaaacc attgacttat ctgttgaaga gcagaaagta attattatat acgctttgat
    18481 acaagccaag gagccagtta aacaatatag tttagcgcaa gaaattggcg tttctgtcca
    18541 gactttagca aagatgttag atgatttaga gctcgattta aataagtacc aactatcttt
    18601 atctcgaaag cgtggcgaag gtatttactt ggtaggtact gaatcaaaga aacgtgaatt
    18661 tttaagtcag ttaatggtga ataacttaaa tagtactagc gtttattcag taattgaaaa
    18721 tcattttgtc tttcattcat taaatcaaat ccacaaagac tttgttgact tggagcgtat
    18781 ttttaatgtt gaaagactat taatggatta cttaagtgcc ttaccctacc aacttaccga
    18841 atcaagttat ttaactttaa ctgtccatat cgtgctctcc atttcacgta taaaaaatgg
    18901 agagtatgtt gcattaaacg atgatattta tgattctgta caaaacacat ttgaacacaa
    18961 agtagcaagc gaacttgctg ataaacttgg tcaaatttat gatgtgacat ttaaccaagc
    19021 agaaattgca tttattacaa tccatctacg tggtgctaaa cgtaaaaatc tcaatgatac
    19081 gtcatcaaat aatcaatgtg aagaaaacaa aattaaagcg tttgttaaaa aagtagaaat
    19141 gatttccggt atgatatttg cagatttgga tactttagtc gatggactga cgctacacct
    19201 taatcctgca atcaatcgtt tgcaagctaa tatcgagacc tataatccgt taacagacat
    19261 gattaagttc aaatatccaa gactatttga aaatgtaaga ttagctttaa atgattgttg
    19321 gcctgatttg atttttccag agagtgaaat tgcttttata gttttacact ttggtggctc
    19381 gattaaaaac caaggtaatc gatttttaaa catattagtc gtttgcagca gtggtatggg
    19441 aactagtcgt ctattatcaa ctcgtctaga gcaagttttt agtgagattg agcgtattac
    19501 acaagcatca gtcagcgatt tgaagtcact agatttaagt caatatgatg gcattatttc
    19561 tactgtgaat ttagacatcg actcccccta tttaacggta aacccattat taccagatag
    19621 tgatatcagt tatgtcgcac agtttttaaa tacaaagtct acgttccaag agacgcatga
    19681 taaatcatca aacatgattg ataaggatga tgttcatgtt gaaacgaaag atattgatgg
    19741 caacacatct tttgaaaatg aacaaacttc atacttaact tcagttttcg aaaaaaattt
    19801 aagtgacgaa aaatcagaac aattaatgca tcatatgcgt tcgggtttaa ctttgcttga
    19861 ttcagtgaaa atagttagta ccgaagttaa acagtggcaa acatacatcg cagattatct
    19921 atatcaatgc gatgtaataa acgatccaac gtcattcgct gaactactag agcaacgatt
    19981 gattgacaat ccaggatgga ttttaagccc atatcctgtt gcaataccac acctaagaga
    20041 caataggatt aaacacccta tgattctaat cacagtttta gaagaaccgt taacattgcc
    20101 tagtattcaa aatgacaatc aaacaattaa atatatgatt tccatgttta ttcctgacaa
    20161 tgattttatg gcatcactgg taagtgactt gtccgaattt ttaagtttgc aattagaatc
    20221 tattgatact tttatggaaa aaccacagga acttgaaaca ttattaagaa acaaattttt
    20281 agaacgaatt aaaaaccaat ttatttagga gtgtaaaaaa tgagcggatt atttagtaat
    20341 gacaatatct ttttaaatgt aaatgttagc agtcaaaacg aagctattga aaaagcaggc
    20401 aaagccttag ttgatagtgg tgctgtaaca gatgcttaca ttcaagcaat gaaagatcga
    20461 gagcaagtgg tttcaacatt tatgggaaat ggtttagcta ttccgcatgg tacagatgaa
    20521 gcaaaaacaa atgtgattca ttcaggttta acgttattac aaattccaga aggtgttgat
    20581 tgggatggcg aagtagttaa agttgttgtt ggcatcgctg gcaaagatgg tgagcattta
    20641 gacttattat ctaagattgc tattactttt agtgaagaag aaaatgtaga ccgtatcgtt
    20701 caagcaaaat ctgcagaaga aattaaacaa gttttcgagg aggcagaagc ataatgaaag
    20761 cagttcattt tggtgctggt aatataggtc gtggatttat tggatatatt ctagcagata
    20821 acaacgtaaa agtaacattt gcagatgtaa atgaacaaat cattaatgca ttagctactg
    20881 aacatcaata tgatgttatt ttagctgatg agtctaaaac aacaacacgt gtaaataatg
    20941 ttgaagcgat taactcaatg caaccttctg aagaattgaa acaagcaatt ttagaagctg
    21001 atattattac aacagctgtt ggtgttaaca tactacctat tattgctaaa tcttttgcgc
    21061 ctttcttaaa agaaaaaaca aatcatgtta atatagtcgc ttgtgaaaat gccattatgg
    21121 caactgatac tttgaaacaa gcggttcttg atattactgg ccctcttagc gataatattc
    21181 atttcgctaa ttctgctgtt gatagaattg taccattaca aaaaaatgaa aatatattag
    21241 atgttatggt agaaccattt tatgaatggg ttattgaaaa agatgcatgg tacggtccag
    21301 aacttaatca tattaaatat gttgatgatt taacaccata tattgagcgt aaattattaa
    21361 ctgtgaatac aggacatgca tatttagcgt atgctggtaa atttgcagat aaagctacag
    21421 ttttagatgc aattgaagat agctcaattg aagctggttt acgccgtgtt ttatcagaaa
    21481 caagtaaata tattaccaat gaatttgatt ttactgaagc ggaacaagct ggttatgttg
    21541 aaaaaataat agaccgtttc aacaattctt atttatctga tgaagtgaca cgtgtcggac
    21601 gaggtacgtt acgtaaaatt ggccctaaag atagaattat aaaaccatta acatatcttt
    21661 ataataaaga tttagaacgc actggtttat taaatacagc tgcattgtta ttgaagtatg
    21721 atgatacagc agaccaagaa actgttgaga aaaataatta cattaaagaa cacggtttaa
    21781 aagcgttctt aagcgaatat gctaaagttg acgatggctt agccgatgaa ataattgaag
    21841 cgtacaattc acttccataa tttattgagc tttgtttgaa acaagaagtt tccaacgtta
    21901 ttcgttaaca atcagtaata atgtagtagt tccgttgaat taacaatatt aaatttctga
    21961 acataaaaaa tactcccttc aacatagata cttaacttgt gttatgtatg aaaggagtat
    22021 ttttgcgtta ataatttgtt tgattttcga gccacagcca cctattcaat ggctattggt
    22081 cattactaaa acaaattcat attaactgtt agacttggtt acttagtaag gaatatttcc
    22141 ctatgaaata actagatgtt cacattcttg aataaatttt attcttcggt ttgttggtct
    22201 ttcttagtga atcttctaat taagaatgcc atacctgcac ctagagctaa ttcagcatat
    22261 ggtaaatcgt cattatgtga cataccagta tctggtaaag ttttagtttg ttgtttaggc
    22321 ttattaactt ttccttgttg ggctgatttt gcatttgctt tgttttcttc agtcttagtt
    22381 acattaagca tatcttgatt agcactattg cttccatttg aaactgtaac taatgacgcc
    22441 ttcatatcat tagtttgcat atctttagct ttattgtttg caactgggcc aactgatttt
    22501 tgcgtatcat tagtatctgc tgttgccgta tcatcttttt ggctaacatt agttgaagtc
    22561 attttatctt ttgcttcaga agatgcagat gttgatggtt tattcgaaac ttcagtatca
    22621 gctttgcttg gcgatttatc tgtttcgtta gatgcaacgt tagtttcaga cttaagttgt
    22681 cctgcatcag tttgatttga cgtactttcc tctttatctt ttgatgtatt agaaggtaca
    22741 tttggttctg ttatgtctgc tgaaggcaat gtttcagttg ttgattcaac catactttga
    22801 tttgttgaat ctctatcatc tttttctgcc ttagctttat tttcagattt tggttgtgca
    22861 accttgccat tagttgattg agattcagca ctattattta cttcagcatt ttgttttgaa
    22921 tcatttacag atgcattatc tttgctatca gcagatgatg ctgcttctgt gctcgcagta
    22981 gttggagccg ttgctgttga tcctgttggt gcattctcgt ttgttgctgt agttgtacta
    23041 ttgttattta ttgtgctttc tgctggcgtt gcattatctg tttctgttac aggtttatca
    23101 gttgtgccgt tattagttga ttctacttct ggtttactag ttacatcgtt atccattgtc
    23161 ggactgtttg ttgatgcatc tacactagaa ttgctattag cttgcggttt atcatttgca
    23221 tcatcagttg ctgatgttgc tgttgtttca cctgttgccg catcactatt atttgatgtt
    23281 gtcggagaag cgtctgcttt gccattatct gtcgtctcag atacgttagg ttgtccagtg
    23341 ttttctggtg ttgcatttga atttgctgtt gcatcattat tatctgtacc attattagta
    23401 tcgttagcat ctggatcatt ctgaggtaca atcgcttcaa ttgctggtat cgttacattt
    23461 tgtaattcag caacttcagc atttgattga actttaacca aatcttcaac aaatctatca
    23521 tgacttctgc ttaaatcatt aagggctgtt tctttcgcgg ctttatttgc atctgcatta
    23581 ttaattgttt ctttttgttt gttaacagca ttttcgattt ctttcaaagc atttttctta
    23641 gcttctggat taatttgttg tccttttagt ttttcaagag cattattttt cacagtttca
    23701 atttctgcat tcgttgtttg gtcagaaata tcttctgttg ctttagataa aatatcttcc
    23761 aaagtatttg taaatgcttc tttttcttca attgttgcat ctgtgttagc attaacattt
    23821 gcttcaattt tgtctaatgc tgtttctaat tcttcaatcg cttgttgttt atgtgttgag
    23881 tcaatttgaa tattatcaaa tgcttcaagt ccttgagctt tcgctttttc cacttcagca
    23941 gttgttgttg catcagtaat acctttttta gcttgatctg taatttgttt aatagttgct
    24001 aatgcttctg ctttttcttc agatgttaat tggtcactat gctcaataga gtcttttgta
    24061 tcttctgctt tagcatcaat tgcttggttc gctttaggtt taacagtagc atctacttga
    24121 acagcgtcaa ttgccgcttt accttgtgtt aaggcatcat ctacatcgcc attatctaca
    24181 ccattattga tgctttctag tgcagtttga acattttggt taacttggtt gattgcttgt
    24241 tgtttttcat cttgcgttgc attagtgtca gctgaaatat tatttttctt ctgatctgca
    24301 taagcatata aatctgttgt agctgattct tttttacctg ttggaattgt gtaatcgtta
    24361 atattatcta agtcattatg gatttgaact tcaatgtcat ctttagaagt agcttgatta
    24421 acattttgat ccgctgtttg ttttaattca gcaagttttt gtttcgcttc agcaatttca
    24481 cttgaagtcg atgcgttaga gttatccgct tcgtttactt ttgaattata tgcagcttca
    24541 atcttagcta aagcatcttt tttgtactca ctaaatgttt taactgcatt aattttagct
    24601 tttccttctt taaccgcatt atcaacatat tcatttgttg atgattgatc gacatttgtt
    24661 ttcgcttgat ttaacgctgc atcaacctct tgttttgcat cattaatttc ttgttgtgac
    24721 gcattaggtg tttgttcaat ttgtgttttc ttatcagttg caacttgatc taattctgtt
    24781 ttacctgctg gcttcttaac aggatttgca tgaagttgtt gaactttttg taccgctgtg
    24841 tctttagcag tagaaacatc acctgttgta tttgcagcat taatattatt taagccttct
    24901 tcatatgctg ctctaactgg ttctatatct tttccttttt cttcattagt agtcgtatta
    24961 tcactaagaa tttcagttat tttattttgc atttcattta gcaattcagc tcttgcattc
    25021 gttttaacat ctgttgctgc ttgaattgga tcaatcgctt gaatcgcatt atcttttgca
    25081 gtgtttacat catcgataga ctgtgcattt tcaatatttt gattaccttg tgttaattgt
    25141 gcgtctactt gttgatttgc ttgttctttt tcttcagtag tcgcatctgc agcttgtgct
    25201 atttgtgctt tttgatcatt tgcttttgtt gctaattcat ctttagcaac atctttaatt
    25261 gttgtatctg cagtaatacc ttgtatatca gcaactgctt gatctttaat ttgtgttaca
    25321 tcatttgttg tttgtgcatt taaaatatct tgatatgctt tttctttagc tttagaaact
    25381 aaatcttttg ctgcattttt ctcttcagtc gttgcacctg tagtattatc aattgcttga
    25441 ttttgagctg tcacagcttg atcaacatca tttttagcat ttgatttaac cgctgttgct
    25501 ggttgtgtgc tttgaattga attctttcca gcgtctttcg cctgatctac accattatca
    25561 tcagttgcag ctgtaatatt atttttcgcg tctgtaactg ctgttgctaa ttgttgaatc
    25621 gctgcttctt tttcttcatt tgttgcgttt tgatcgttat taataaccgc tgtttgtgct
    25681 gcttgtaatt gatcaatctc atctttcgct gcttgtttct tcataacttt tggtgttaca
    25741 gcgttaattg ccgtttctgc attagttttt gcctcatcta cttctgcatt tgttgtagct
    25801 gctgtgatga cttgatttgc tttagcattt tcagtattag cttcggtttc tgctgcttgt
    25861 ttttcttcat ctgttgcatc tggcgtagct tgaatctctt gtaacttgtt atttaaaact
    25921 gttgtgattt cattacgtgc agttgctttt ttattaacag ttggtgttac ttggtcaata
    25981 ctagtctcac catttgtttt agcttgatca acatcatttt gactatttgc agcttcaatg
    26041 ttgctatttg cttgtgtcgc tgcattatct acatccgcat tagctgctgc aatttcttct
    26101 gcagtaatgt cttgcgtttg agcgattgct gttttacgtt cattcgcttt cgtagcaatt
    26161 gcttgtttcg catcatcttt cgttgttgtc gctggttgaa ttgcttcaat tttagcaatt
    26221 tctgcatttt tagccgcttc aacttccgca tttgaatgtg caccatctat tgctgcatca
    26281 gctgtcgttt tttctgtttg aacttgttgt ttcgctgctg ttttctcttc tgttgttgca
    26341 ccgttattag catcaattgc tgtttcttgc gcttgtactt tatctgcaat tgcttgtttc
    26401 gctgttggtt taacatttgc atctggcgta attgcagcaa ttgttgcttc atttgtagtt
    26461 tttgcattat ccacatcagt attagctgct gcgttatcaa tatcagcatt agcagtaact
    26521 actgcttgat caactttatc ttttgcagct tgttgttctt cagtagttga agcattcatc
    26581 gcttcaattg ctgttttacg ttcacttgct ttttgagcga tttctgcttt agcatcagct
    26641 ttcttagttg ttgctgcttg aacttgatta attgcagcaa tagcattatc tttagctgtt
    26701 gctacatcac tatttgagtt tgctgcatca atattcgttc ttgcctcttg ttttttagca
    26761 tccaattctg catatgcagc ttctttttct tctgttgttg aagcattact attttggatt
    26821 tcttgtttac gtgtattata tgcgttttct actgctgcat cagctgctgg tttaacccta
    26881 gcttgcgttt gaattgcatt gattttatct aaaacttttg ttttagtttc agcaacttct
    26941 tgttgtgatt ttacaacttg gatgtcatgt aatccttctt tttgagctgc atctactgct
    27001 gcatcagctt ctgctacttc ttcatctgtt gcatttgaaa ctgttgcatt ttgagttttt
    27061 cgagctgttg cagcttgttt aacttcttta tatgcaccca ttttagtttg agttgagtct
    27121 gtaatattct caattttgaa aatttcttca tttttaactc gttctaaatc ttgtgcagtc
    27181 gtagtcgctt ctatcgcttt aacacctgaa acttttgctg cattaatacg ttcaatagct
    27241 tctgctactt cctcatttgt tgtatcagga tttaaaggtg cctggtcaat ttgagcttgt
    27301 acaacttcgt caaattcttc aagagctgct gctttagctg atgtagaagg tgcagtttga
    27361 ttaatatcat tgattgcttt cgttttaatt acttctactt gtgcatttgt tgttgctgca
    27421 tcaatatctt taattgcctt tgtttcaatt ttaccaattt tatcatttgc tacatctttt
    27481 tcatcttgta atgacgcatt tgacttttta atttgttgtt tacgagttgt aactgcttgg
    27541 ataatatctt gtttcgcttg aggtttaact gttggcgtaa tcacatcttg ttctaacact
    27601 gcgataccat tatctttttc agttgtaaca ccttgtgctg ttgtttgttt attaatttct
    27661 gcgattgcat tatttttatc attgttaatt ttagtcacaa gcgctgcaac ttcatcttgt
    27721 gtaacttttt tactttgttg tgctgctgta accttttctt gagctttagc atctaattca
    27781 gcaattgctg cttgattatc aattaatgca gcttgtaatt tatttactaa accgtcaata
    27841 tccgcttgtg atgcacgatt tgcagtttca acatgattcg catcttcatt taaaatagtg
    27901 tctgcttgtt gtttaagttt attgtattct gcaattgatg ctgttgtgta atgactatta
    27961 tcaacttgtg agtttacttg ttgttgcaac gcatctttgt tcatttcaac cgctactgaa
    28021 aatggatctg cagttagtgt aacttctggt tgcgcattat taattacaac atctgaagca
    28081 gtacgatatg ttaatttttc attaaaatca atatttttag gtgtatcaat attagtaaca
    28141 ttaactttat atgataattt taaagattta tccgggaata aaacttcttt agtgtgtgta
    28201 ccacgtgccg ttgtcacacc ttggcttgta aatgtaactt tatttgcatt ttgatcataa
    28261 ttaacagtca tatttttcaa tactgtactg tcttcattac cattagggaa tgttgtagtt
    28321 aatgaatcat taacgtaagt tacaccttgt ggtaatgtta cttcatattt aaattgatct
    28381 gcttttaatg aagcaccaaa attaccattg ttaccgattt cagtattaat agtatattct
    28441 ttattgttag ttgctttcgg tgctaaatct ctatttttga catataaatg tgatcctgag
    28501 aatagaccta cattatcaat gaatgaatag tatttataac catctttatt tgttgtaatt
    28561 cttgaagcat cagcaactgc tgtattgtca ggaataaatt gtaaatctaa acgatcagca
    28621 tccgttggca ctttaaataa gtgtgcagtt ccagtcttag caacatttac agttgctaat
    28681 tcagtatttt tcttagcatc tttaataact aaatttgttg caccttgctt actattcgtt
    28741 tgcatagtag taaagttaat aattaattct gaatctggtt ttacatttac agtttttgaa
    28801 ataccgttaa agttaccatg atctgtagaa tcatttgcat tcacacgacc taatgcagcc
    28861 acgtttcctt tagcttggta gttttgaggg ttattcttat caaacatatc gcttcgtctt
    28921 aattctgagt taacgaaacc aatcttacca ttgttaatta atgaataacc atttacttta
    28981 tctgtaacag ttacagttgg atcctgtcta ttctcatctg ttgatatggc aggatcatca
    29041 aatgttaatg tcgtattaat actgccttca ccagtattgc tagcatttgg atcttgagtt
    29101 tgtacgtttg ctgctacagg tgctgctggt tgcactgctg ctggagcatt tgctggctgt
    29161 gtttgatttg ccggtgttgc attattatta ggtgttgctt ggttatttcc ttgacctgct
    29221 tggtttgccg gtgctgcttg atttccaggt tgtgcttgtg cagcgttatt cggatcagct
    29281 tgattacctt gtccagctgg ttgtgtattt ggttgtgctg ctcctcctgc tggattagcc
    29341 tgtccacctt ggtttgctgg ttgtgctgct ggttgtcctt gattggcagg tgcagctggc
    29401 tgtgctgcag gattagcttg agcaccagca tttgcgttag gctgtgtatt ggcatcagct
    29461 ggttgtgctg gttgatttag tgcaggctga ttttgctctg ctgcagacgc tgttgtcggg
    29521 ttagtagata taaaagtaac agtggcaatt aaagctgaaa aaataccgac attaaatttt
    29581 ctgatactaa atttttgttg tctgaataaa ttcatttagt catcctcctg gttgattatt
    29641 ctcgctgtta aatgatttca cttaatcaac tgttaagata agtagtagca tctgcgttaa
    29701 aaacacaaag caactatatc taattaaaat taattttatc atcattatat attgagtacc
    29761 agtgtatttt atattacata ttgattactt tgtttttatt ttgtttatat cattttacgt
    29821 ttgtactata aattatttct acaaacacaa aaaaaccgat gcatacgcat cggcttattt
    29881 gtaatacagt atttatttat ctaatcccat tttatcttga accacatcag ctatttgttg
    29941 tgcaaatctt tcagcatctt catcagttgc tgcttcaacc atgacacgaa ctaatggttc
    30001 tgttccagaa ggtcttacta aaattcgacc ttctccattc atttctactt ctactttagt
    30061 cataacttct ttaacgtcaa cattttcttc aacacgatat ttatctgtta cgcgtacatt
    30121 aattaatgat tgtggatatt ttttcatttg tccagctaat tcacttagtg atttaccagt
    30181 catttttatt acagaagcta attgaatacc agttaataaa ccatcaccag ttgtattgta
    30241 atccatcata acgatatgcc cagattgttc tccacctaag ttataattac cgcgacgcat
    30301 ttcttctact acatatctgt cgccaacttt agttttatta gatttaattc cttcttgttc
    30361 aagcgctttg taaaaaccta aattactcat aacagtagaa acaatcatgt cattattcaa
    30421 ttcttgattt ttatgcattt cttgaccaat aataaacata atttggtcac cgtcaacgat
    30481 ttgaccattc tcatctactg ctatgattct gtctccatcg ccgtcaaatg ctaacccaaa
    30541 atcactttca gtttcaacaa ctttttctgc taatttttca ggatgtgtag agccacattt
    30601 ctcattgata ttatatccat caggactaca tccaattgtt tcagtatctg cttctaaatc
    30661 accaaataag aatggcgcta atgatgatgt tgaaccattt gcaccatcta aaacaatttt
    30721 caaaccttca aagttaacat ctactgttga ttttaaataa ctcaaatatt tttgtgcccc
    30781 ttcaaagtaa tctgaataat gtacaatatc attgccaact ggtcttggta attctgggtt
    30841 ttcttgatcc aataatgctt caatttcatt ttcttgttca tctgatagtt taaaaccatc
    30901 tgatccaaag aatttaatac cattatctgc aactggattg tgagaggctg atatcattac
    30961 acctaactct gcacccatat cgcgtgttaa atatgcaaca cctggtgttg aaataatacc
    31021 taatcgcatc acttctgcac caattgaaat caaaccagct attaatgctg attctaacat
    31081 ttcacctgaa actctagtat cgcgtcctac aagtacacgt gggtgttttt cacctttatt
    31141 atgtgctaga acatagccac cgtatcttcc taatttaaat gccaattcag gtgttaattc
    31201 ttggtttgcg acacctctta ctccgtctgt accaaaatat tttcccattg tttactctcc
    31261 tttaatttag ctatttattt tacatttata taagccttcg tttcacttgg ttgtgcttta
    31321 gtgacatgtt ctggtaaatt gatttttaca gtcttttcag ttgattctga aataccatct
    31381 aaatctactt ctgcatcaac ttcgcttata ttttgcaagt catctcgact accgaagatt
    31441 tcaatttctt tatcttctaa gtcaatcgaa cttaattctt tatcatctgc taaactacct
    31501 ttctgtttaa catttacttt aacctttttg ctaaatggct ctactttaac ttgtaaattc
    31561 acttcattag gttgaatcga tacatttaat ttattcagtt ttttatcaaa agccgttact
    31621 tctgcgacat cttttgtgtc accattaatc tttttattag ttttaaaagt ggcttttaaa
    31681 taagcaatat cattcaattg ttcttctcca cctgttactt taactgtttg tggtgaaact
    31741 tcttgctttg taattttata aagtggatca atatcactct gacttacatc aggttgaact
    31801 gtcatctttt tagttacttt gttttcaaga gtaatatttg ctaatttagg ttttacagaa
    31861 taatgaatgt catcagctaa ccctttaact tgatacttct cttgatactt tccagcttta
    31921 gcatttgata aatcaatcac tactcttaaa tcttctggat tttcaatttt tattatcttt
    31981 gattgtggtc ctgaaatagt cacattaact gtttcaggcg cttttgttaa atgcaaatct
    32041 ttagtgttat aaagaatttc aacgggtaca tcttgaatcg ttttactaga cttttgacca
    32101 agattaccag tgttaaagat atttccaaaa acattgttaa cagataaaaa gaaaaacaat
    32161 gccaaaagaa aggcaataaa tctcaagccc catttacttt ctagcatatt atttcacacc
    32221 tttcttttga aagcgtgtgc caaaccaatg ttcagcaagc aactcttcaa aaatttcgtt
    32281 tgaaatgtct cgtcgtaatt ttccatcaaa tgttaccgaa atatcaccag tttcttcaga
    32341 tacaataacg gtaaatgcat cagatacttc tgaaatacca accgcagctc tatgtcttgt
    32401 acccaaactt ttagatatct taggactatc agacaatggc aaataacttg ctgctgctgc
    32461 aatcttcgtg ccttgaataa tcattgcacc atcatgtaaa ggtgtattag gtataaagac
    32521 attaattaaa agttcttgcg aaatatttga atccattgca atacctgttt caatataatc
    32581 ttgaagacct gtttcttttt caaagacaat taatgcacct atacgtcttt tagccatata
    32641 ttgcacagcc tttgaaaccg attgaatcaa tttctcttca tctttactat acgtattaga
    32701 agtatagcgt tttaaaaagc tacctctacc aagttgttct aacgcacgtc taatttctgg
    32761 ttggaatatt actattaaag ctagtacccc ccattgaata acgatatcga ataatttaga
    32821 tgttgcagtc aagttcaata tcatacttat ctgctgacca ataacaatta ctaatatccc
    32881 tttaagtaat tgtatcgctt tagttccctt aaagaccgtg atgagaagat aaagtacata
    32941 ccaaactatc agtaaatcaa ggatactcgt tacaattttt aacgtactga ggttttgaaa
    33001 aaagttggaa aaatccataa catctcctcc gggtaatatt tttccataat acccattata
    33061 ccaatcattt acatcactgt catacgaaca tata
//
LOCUS       DAHHRR010000018        32456 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.018, whole genome shotgun sequence.
ACCESSION   DAHHRR010000018 DAHHRR010000000
VERSION     DAHHRR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32456
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.018"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..878
                     /locus_tag="NBI42_000693"
     CDS             <1..878
                     /locus_tag="NBI42_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCD1777133.1"
                     /translation="LLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIFRLEASEH
                     KIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPIPNTVSDL
                     SPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLPILTISAI
                     LAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTLFAPIQAT
                     VAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMTLKYSIIF
                     VAFICVWTFILTLIF"
     gene            complement(1201..2703)
                     /gene="spa"
                     /locus_tag="NBI42_000694"
     CDS             complement(1201..2703)
                     /gene="spa"
                     /locus_tag="NBI42_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HCD1777134.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDGNKPGKEDGNKPGKEDGNKPGKEDGNKPGKEDGNKPGKEDGNKPGKEDGNKPGK
                     EDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIKPGQELVVD
                     KKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL"
     gene            complement(3124..3876)
                     /gene="sarS"
                     /locus_tag="NBI42_000695"
     CDS             complement(3124..3876)
                     /gene="sarS"
                     /locus_tag="NBI42_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HCD1777135.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDHIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
     gene            complement(4245..5243)
                     /gene="sirC"
                     /locus_tag="NBI42_000696"
     CDS             complement(4245..5243)
                     /gene="sirC"
                     /locus_tag="NBI42_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCD1777136.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSIDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTVLVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(5240..6235)
                     /gene="sirB"
                     /locus_tag="NBI42_000697"
     CDS             complement(5240..6235)
                     /gene="sirB"
                     /locus_tag="NBI42_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCD1777137.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALTGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(6251..7243)
                     /gene="sirA"
                     /locus_tag="NBI42_000698"
     CDS             complement(6251..7243)
                     /gene="sirA"
                     /locus_tag="NBI42_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCD1777138.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            7474..8454
                     /gene="sbnA"
                     /locus_tag="NBI42_000699"
     CDS             7474..8454
                     /gene="sbnA"
                     /locus_tag="NBI42_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCD1777139.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            8451..9461
                     /gene="sbnB"
                     /locus_tag="NBI42_000700"
     CDS             8451..9461
                     /gene="sbnB"
                     /locus_tag="NBI42_000700"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCD1777140.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            9482..11236
                     /gene="sbnC"
                     /locus_tag="NBI42_000701"
     CDS             9482..11236
                     /gene="sbnC"
                     /locus_tag="NBI42_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCD1777141.1"
                     /translation="MQNHTAVNTAQTIILRDLVDALLFEDIACIVSNSEITKENGQTI
                     LIYKRETQQIKIPVYFSALNMFRYESSQPITIERRTSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTAPIKRQATDMLHDKGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDISAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIARVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            11229..12485
                     /gene="sbnD"
                     /locus_tag="NBI42_000702"
     CDS             11229..12485
                     /gene="sbnD"
                     /locus_tag="NBI42_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCD1777142.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMAWCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVIGQIIGSLSGAAITSYTTPATTFIVMGIVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            12475..14211
                     /gene="sbnE"
                     /locus_tag="NBI42_000703"
     CDS             12475..14211
                     /gene="sbnE"
                     /locus_tag="NBI42_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCD1777143.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYAEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPIHPYQINVLRQYPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPTSKLSQVIEQSGLAAE
                     AWLECYLDRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPEVCYVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKASRNEVVLWKLV
                     AHRLMTWKKEYANNAVFVACVEDLYQKPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            14120..15970
                     /gene="sbnF"
                     /locus_tag="NBI42_000704"
     CDS             14120..15970
                     /gene="sbnF"
                     /locus_tag="NBI42_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCD1777144.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVIYKTSYQDGVGHFTIEGQDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKLETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDTYLKDELKTAFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYDIDEKLQWKWVKGIIEAYQEAFPELNN
                     YKHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGITDATTISET"
     gene            15945..16721
                     /gene="sbnG"
                     /locus_tag="NBI42_000705"
     CDS             15945..16721
                     /gene="sbnG"
                     /locus_tag="NBI42_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCD1777145.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDHETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDED"
     gene            16721..17923
                     /gene="sbnH"
                     /locus_tag="NBI42_000706"
     CDS             16721..17923
                     /gene="sbnH"
                     /locus_tag="NBI42_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCD1777146.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLAFKPTNHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDEVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKYRF
                     PLKHINLGGGIGVNYADLTSQFEWDNFVENFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHDAWYAILRGGTQQFRLPVSWQHNHPFEIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            17927..18691
                     /gene="sbnI"
                     /locus_tag="NBI42_000707"
     CDS             17927..18691
                     /gene="sbnI"
                     /locus_tag="NBI42_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCD1777147.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            18887..19315
                     /locus_tag="NBI42_000708"
     CDS             18887..19315
                     /locus_tag="NBI42_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777148.1"
                     /translation="MNMNLIIGKIAIWIGIVVQIFYSVVIVSITTFILFINAKGASPE
                     IAVLIALVIILILTLPPILTAINLENKMEVKGTLFIVYAIVAFCMFNYLSSILWVVCG
                     IFLIWTKYSKGESTDDDENEKVDIESTENQFDSKDKITKE"
     gene            19365..20159
                     /locus_tag="NBI42_000709"
     CDS             19365..20159
                     /locus_tag="NBI42_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1777149.1"
                     /translation="MRKNIELILIWMGIYIQIAYMAIIALLIYIFTLFFGSFFGFLFL
                     LTGNEIGDLLKTPTSFMIFATCLIIFSVLPVTVLAFDLSKRRILKGIILVVYAIIAAL
                     LYNFVSAILWFIVAISLFIRKKSLSAENTVAVQSHKGASKQEIRYKLVYKSVSIKHSD
                     DSIKVAHTVGNKKLIDDKTIDIHLDSSEILKKISTDANQASHKDTCKKELDSQDMMEH
                     PEFKNPTTKNLEGLNEEIHKDEATTKVDSDNTEPPIESKDHVSKKD"
     gene            20371..21147
                     /locus_tag="NBI42_000710"
     CDS             20371..21147
                     /locus_tag="NBI42_000710"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCD1777150.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            21291..21413
                     /locus_tag="NBI42_000711"
     CDS             21291..21413
                     /locus_tag="NBI42_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777151.1"
                     /translation="MHQLKALLVLTHQRYYKTSQKPHYLIYLIKNSQSYLILFL"
     gene            21483..22463
                     /locus_tag="NBI42_000712"
     CDS             21483..22463
                     /locus_tag="NBI42_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCD1777152.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNSHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            22426..23118
                     /locus_tag="NBI42_000713"
     CDS             22426..23118
                     /locus_tag="NBI42_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCD1777153.1"
                     /translation="MKKLQLKKWKCHKNNIEDVHINKNYNYQRKLDALEEVRKGYYPI
                     KRAIDLVLSIVLLCLTFPIMFIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            23328..24494
                     /locus_tag="NBI42_000714"
     CDS             23328..24494
                     /locus_tag="NBI42_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCD1777154.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHHDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRVVFTAHGWAFTEGVKPAKKFLYLVIEKLMSRITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVTDNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            24475..25713
                     /locus_tag="NBI42_000715"
     CDS             24475..25713
                     /locus_tag="NBI42_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCD1777155.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYVHNYVFKVIACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMLVIMLILLCFTFYNINYYLFQLSDLDGLPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            25703..27133
                     /locus_tag="NBI42_000716"
     CDS             25703..27133
                     /locus_tag="NBI42_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCD1777156.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLLLSLTVFAMYI
                     AVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASVIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFISVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQK
                     SHSLTKTLANNMNIQLILTVPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
     gene            27401..28000
                     /locus_tag="NBI42_000717"
     CDS             27401..28000
                     /locus_tag="NBI42_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCD1777157.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            28370..29095
                     /gene="sasD"
                     /locus_tag="NBI42_000718"
     CDS             28370..29095
                     /gene="sasD"
                     /locus_tag="NBI42_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCD1777158.1"
                     /translation="MKKLATVGSLIVTSTFVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(29286..30041)
                     /locus_tag="NBI42_000719"
     CDS             complement(29286..30041)
                     /locus_tag="NBI42_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1777159.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            30277..30984
                     /gene="deoD"
                     /locus_tag="NBI42_000720"
     CDS             30277..30984
                     /gene="deoD"
                     /locus_tag="NBI42_000720"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD1777160.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            30991..32343
                     /gene="tet(38)"
                     /locus_tag="NBI42_000721"
     CDS             30991..32343
                     /gene="tet(38)"
                     /locus_tag="NBI42_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCD1777161.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDFNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
BASE COUNT        10758 a         4988 c         6071 g        10639 t
ORIGIN      
        1 aattattatt aacaatattc catggtccag aactagcaga cattattcca tcactagcaa
       61 caatggtggt gttagcattt gtttgtcgta aatttaaacc gaaaaacatt ttcagattgg
      121 aagcgtctga acataaaatt caaaaacgaa cgcctaaaga aattgtcttt gcttggagtc
      181 cgttcgtcat tttaactgcc tttgtattag tatggagtgc accattcttc aaaaaattat
      241 tccaacctgg aggtgcactt gaaagtctag taataaaatt gccaattcca aatactgtga
      301 gtgatttatc gcctaaagga attgcgttgc gtctcgattt aattggtgca actgggacag
      361 cgattttatt aacagtaatt attacaattt taattacgaa gttaaaatgg aaaagtgcag
      421 gtgctttatt ggtcgaagca attaaagaat tatggttacc gatccttaca atttcagcta
      481 tcctagctat tgctaaagtt atgacatacg gtggtttgac tgtagcaatt ggacaaggta
      541 ttgctaaagc gggagcaatt ttcccattat tctctccagt attaggttgg attggtgtgt
      601 ttatgactgg ttcagttgta aataacaata ctttatttgc gcctatccaa gcgacagtgg
      661 cacaacaaat ttcaacaagt ggttcattac ttgtggcagc caacactgca ggtggcgtag
      721 cagcgaagct tatttcacca caatcaattg ccattgcgac tgcagctgtt aaaaaagttg
      781 gtgaagaatc tgcattatta aaaatgacat taaaatacag tattatattt gttgccttta
      841 tttgtgtttg gacgtttata ctaacgttaa tattctaaat ataaataatg ttgtcacttg
      901 gattcaaatg acattttaaa tctaattatt catgaatcga gctagtacga gatactatat
      961 gtatttcact agttcgattt tttacgtttg gaaacacttg ttgtgtctat agatttcgcg
     1021 atttgtttct tcgatggcag tcagaaatat gaggccaata aaaaatgcac tgagcaacaa
     1081 aagatgttgc tcgtgcattt agatgattct tatcatttca aataagaatg tgttaatcaa
     1141 cgtatataag ttaaaattgg tttggataaa atgatatcta tcgttgtgta ttgtttgttt
     1201 ttatagttcg cgacgacgtc cagctaataa cgctgcacct aaagctaatg ataatccacc
     1261 aaatacagtt gtaccgatga atggattttc ttcaccagtt tctggtaatg cttgagcttt
     1321 gttagcatct gcatggtttg ctggttgctt cttatcaaca acaagttctt gaccaggttt
     1381 gatcatgttt ttatcagcta atttgttatc tgcagcaatt ttgtcagcag tagtgccgtt
     1441 tgcttttgca atgtcattta ctgtatcacc aggtttaacg acatgtactc cgttgccgtc
     1501 ttctttacca ggtttgttgc catcttcttt accaggtttg ttgccgtctt ctttaccagg
     1561 tttgttgccg tcttctttac caggtttgtt gccgtcttct ttaccaggct tgttgccgtc
     1621 ttctttacca ggcttgttgc cgtcttcttt gccaggtttg ttgccgtctt ctttaccagg
     1681 cttgttgccg tcttctttgc caggcttgtt attgtcttcc tcttttggtg cttgagcatc
     1741 gtttagcttt ttagcttctg ctaaaatttc tttgctcact gaaggatcgt ctttaaggct
     1801 ttggatgaag ccgttacgtt gttcttcagt taagttaggt aaatgtaaaa tttcatagaa
     1861 agcattttgt tgttctttgt tgaatttgtt gtcagctttt ggtgcttgtg catcatttag
     1921 ctttttagct tctgctaaaa ggttagcgct ttggcttggg tcatctttta agctttggat
     1981 gaaaccattg cgttgttctt cgtttaagtt aggtaaatgt aagatttcat agaaagcatt
     2041 ttgttgttct ttgttgaatt tgttatccgc tttcggtgct tgagattcat ttaacttttt
     2101 agcttctgac aataggttag cactttgact tggatcatct tttaagcttt ggatgaaacc
     2161 attgcgttgt tcttcgttca agttaggcat gttcaagatt tcatagaaag cattttgttg
     2221 ttctttgttg aaattgttgt cagctttcgg tgcttgagat tcgtttaatt ttttagcttc
     2281 acctaaaacg ttagtacttt ggcttggatc gtctttaaga ctttgaatga aaccattgcg
     2341 ttgcgcttcg tttaagttag gcatgttcaa aatttcatag aaggcgcttt gttgatcttt
     2401 gttgaagtta ttttgttgcg catcagcttt tggagcttga gagtcattaa gtttttgagc
     2461 ttcacctaaa acgttagcac tttggcttgg atcatcttta aggctttgga taaaaccatt
     2521 acgttgatca gcgtttaagt taggcatatt taacacttga taaaaagcat tttgttgagc
     2581 ttcatcgtgt tgcgcagcat ttgcagcagg tgttacgcca ccagatataa gtaatgtacc
     2641 taaagttaca gatgcaatac ctacacctag tttacgaatt gaataaatgt ttttcttttt
     2701 caaattaata ccccctgtat gtatttgtaa agtcatcata atataacgaa ttatgtattg
     2761 caatactaaa atctatattt ataattaaat ttaaatgtaa gttttacaac ttataaaata
     2821 aatattttgc tcaaagattt attcaggaag taaaaggctt aaaaccacaa aatcacgcta
     2881 tttcgattaa taaaaacaga taaaatatag agatattttt tataatgaaa gcggtttaat
     2941 ttatacgagt taaaaaatat ttttaagaat aaagtggggc tttgaatgtg ctgaggttta
     3001 atttcggaaa tggttttaag agatatagaa aatagaaagt tgttcataat gtgctgaggt
     3061 tttattaaaa aatagaaaac acaagtgcac tttagaagac agtacacttg cgtaatagag
     3121 atattattca aaaacaagat gtaaatgatc tttatctgct aataattgat tcacttgagc
     3181 taataattgt tcagcatggt cttgctgcgc gtcatccata tgaattaaaa tttttctttc
     3241 atcttcagtt gagcgttctt ttattagata gccttgcttt tttaaattat tgagagctct
     3301 aacagtttga gggtatttat ggtggattgt ttcaattaaa tctttaagaa gaacgatgtt
     3361 tttattttga gaagtgataa tagctagaat tgtgaattct acaaaactta atgttagatg
     3421 ctttttgata atattcttga aatacattgt atacatcatc aagtttagaa attctttact
     3481 atcttttggt atcatctgtg attcactttg atctgcaagg ttaaattgtt taatgatgtg
     3541 atcaaacaat gtaacacgtt ctgcaatttt ctctcgttgt tcttcagata ttgaaatgta
     3601 agtattacgc tcatcaattt tacttcgaac tttactaata tatgaatgtt tcacaagtac
     3661 ttttatatgc tgtactaaat ccgattgttt ataacataaa tctgaaacaa tcttcttaaa
     3721 tggaagtgtg ttttcttgct gatgaaataa ataagtcagt aatataaatt cttttatagt
     3781 catatcgact tcaggcttga cttttttctt aaaacgaaac atatatgctt caatgattat
     3841 aaaatctcta attttgtcat ggttattata tttcattgtt ttatctcctt gtatatgcac
     3901 tttattttaa tataacacaa tataataaga aaaataatcc ataatttaca attaaaaata
     3961 atatgatatt tttattaaaa tttgtgtttt tgaataaatg acattatcaa acactatatt
     4021 taaataagaa atagttatcg atatttgaaa aatcgaagaa tattgattga tattatagaa
     4081 tatgagtcgg tgttatttgt atataaagta ttaatatgta attaagaaag tcgacgcttg
     4141 ttaacaataa cgacaactat atgtataaaa aaacctcgca actgttagtt agctgaatca
     4201 ttttgctaac gttgttgtga ggtcattttg ttttaataat atcgttataa ctttttcacg
     4261 gttaataata agtatatgaa gaatggggca ccaaaagcag caataaatac acctgctggc
     4321 acttctttag gcaagaataa ggtacgccca attaagtctg caataacaat tgatatggca
     4381 ccaatcattg ctgacattag taacttttta gcataacttc cgcgaacgat tgttttcgcg
     4441 atatgtggtg cgattaaacc gacaaaccca atgttaccta ctaaactgat tgccatagat
     4501 acgagtacag tagaagtgat taattggatt agtttcatac gttgtacatg taagcctaag
     4561 ccaatcgcta cagggtcatc aagtatagat attttcattt ttggtataac aagaaataac
     4621 aacggcacaa cagctaaaat aaccataccc aaaatgattg tatctttaaa cgtagcaccg
     4681 taaagacttc cgactagcca tgtataagct ttggcagcag ataattgctt cgttgtaatg
     4741 agtaatcctt ggacaagcgc aataaacaac gtttgcatcg aaataccgat gattatgagt
     4801 gttgtcgggc gtatttgtcc tttcgtttga aacactaata gtatcatcat tgcaactgcg
     4861 ccacctaata ctgcaaatag tggaagtaaa tgtatcgtta aatggctgaa aaatgcaata
     4921 aagacaacag cacttaagct agcaccacct gtgattccga taatatctgg tgaggcaatt
     4981 ggatttttca atacattttg taacattaaa ccactcatcc ctagtgcggc accagctaat
     5041 atcgcaagtg taatgcgagg taagcgtaat acttctaaag tgaattgatc tatactgtca
     5101 tttggattta taaagtacat caatacgcgt tgtaatggta taaagcttga accaatcatc
     5161 atacttacca ctgaaacgat ggctaaaaag attaacgcga agatgagatg gtaattgtct
     5221 tttttattaa tcttttcggt cataagcgtt gacgtccttt cttcataata tagattaaga
     5281 caatagcgcc aatgacagcg gtaacgacac cgataggcaa ctctagtggc ttaattatta
     5341 tacgagcaac aatatctgaa atgatcatta ggattgctcc agttaatgca gtaaaaggaa
     5401 ttaaatactt atagtttggt ggcaataatc gtttactaat attcggtacg ataagaccca
     5461 caaagacaat tgatccagct actgctaccg aaataccggc taacatactg atgagcataa
     5521 taatcatcca tttgattaat tttatgtttt gaccgaggcc ggttgcaatg tcgtcacttg
     5581 tcatcaagat gttgatgtgt gcagccatgc taaatgcaat taaaataagt atcaatacaa
     5641 gcggaataat ccatgggata tcccaaatgt tacgtaatga aacggagcca ctcaaccaaa
     5701 ataataggcc ttgtaagtct gtttcgttca taataagtat gccttgagta aaggctgtaa
     5761 atagcatcgc aatcgcagca cctgccaaaa tgacgcgatg aggtgaaaat agggtttgtc
     5821 taaacatacc tagtgcaacg actaagactg taacaacaat agcccccaaa aatgcgatga
     5881 caacaatcat tttaaaagat tgaatttgga taaatgtaat actaaagatg acaaaaaata
     5941 ctgcgcctgc attgacaccg aaaagccctg gtgatgctat tgggtttcgt gtaagtgctt
     6001 gcatcaacaa acctgagaca gcaagggcag caccagtcaa taacgcaatg attgttctcg
     6061 acgcccgtgc accagtgaca acatcatgta aatcgttttc actatcaaag ttgaataacg
     6121 cctgtatcac cgtacctggt gacacaagcg tatttccaat cattaaactt aagatagcta
     6181 ctattgcaag acataaacca gcaataacga tttggtattt tggtttaagt agcatcgtaa
     6241 aactccttaa ttattttgat tgtttttcaa tatttaactt ttcatataaa tcgtcaataa
     6301 gttttaatga tgatttatat ccgccagcta agttccaagt gatttcatct aaatcatcag
     6361 atacttggtt gtttttaact gcgtctaaat ttttccactc tttacttgaa gtccattcgc
     6421 tttcagtctt tttaactaat gcagcatctt tcgcatttgg atctgatttt actacaaaaa
     6481 tatgatcagc gttcattaat ggaatgcttt ctttagatgt aagttggata atatctttac
     6541 cattatcaac ttgtttttgt aagtctttat tacgtttgaa tcctaaatca tttaagattt
     6601 caccagcata tccaccagca taaattcttg tatgatcagc acggaagtta acaactgaag
     6661 ctttcaatgg ccatgcatct ttatactttg cttttgcatc tttttggaat gcagctactt
     6721 tatcatcgta ctttttaagt aaatcttcag cttctttttc tttccctaaa gctttcccca
     6781 ttaacttagt tgtatctttg aatttgaaaa ctgtatcagt agaaactgtt ggtgcgattt
     6841 tagataattg atcgtaaact ttttcatttc taacttttga cgcgacaatt aagtccggtt
     6901 ttaatttaga gatttcctct aagttaggcg caggttcttg acctacaatc ttagtatctt
     6961 ttaaatcatt ttttatgtat tcgaatttcg gtttttgtgt ccatgattct acagcaccta
     7021 caggtttaac acctaaagat acagcgacgt cagtggcacc ttgatatagc gtaacaacac
     7081 gctttggttt ccctttaatt tcagttgtac ccattgcatg tttaattgaa gttgtttcct
     7141 tatctttgtt atcagatgat tgcttatttg aattcccact acatcctgct aaaacaagta
     7201 ggaaagcaag cgtaacaaca agcattttaa ttactttatt cattgactaa ttagcctcct
     7261 tcgtgatgta tgacaatgag aatcattatc acgatttagt atgaattaaa ttttttccta
     7321 agtcaataaa atatttatga tttacatgca atttataatt atttgacata taaatgcata
     7381 aaaaatataa tcctaattac ttgatagtga gaatcattat caattaggta acacacaata
     7441 ttatagaatt ttaaatttga ggaggaagcg cttttgattg aaaaaagtca agcatgtcac
     7501 gattcattgt tagattctgt agggcaaaca cctatggttc aacttcatca actatttccg
     7561 aaacatgaag tgtttgcaaa gttagagtat atgaatcctg gaggcagcat gaaagatcga
     7621 cctgccaagt acatcattga acatggtatt aaacatggtt taatcactga gaatacacat
     7681 ttaattgaaa gtacttctgg taatttaggc attgcgttgg caatgatagc taaaatcaag
     7741 ggattaaaac tcacgtgtgt tgttgatcct aaaatatcac caacaaattt gaaaattatt
     7801 aaaagttatg gtgccaatgt agaaatggtt gaagaacctg atgcacatgg gggttattta
     7861 atgactcgta ttgcaaaggt gcaagaactg ttagccacta ttgacgatgc atattggatt
     7921 aatcaatatg cgaatgagtt aaattggcaa tcccattatc atggtgcagg cacagagatt
     7981 gttgaaacaa ttaagcaacc tatagattat tttgtcgcgc cagtcagcac gacaggtagc
     8041 attatgggta tgagtagaaa aataaaagaa gtgcatccaa acgcacaaat tgttgctgtt
     8101 gatgcgaaag ggtcagtcat ttttggtgac aaacctatta atagagaatt acctggtatc
     8161 ggtgctagtc gtgtacccga aatattgaat agatcagaaa ttaatcaagt gatccatgta
     8221 gatgattatc aatctgcttt gggctgtcga aaactgattg attatgaagg catatttgcc
     8281 ggaggttcaa caggttcgat tattgcagcg attgagcagt tgataacgtc aattgaagaa
     8341 ggtgcaacaa ttgtcacgat tttaccagat cgaggcgatc gttacttaga cttagtttat
     8401 tcagatacat ggttagaaaa aatgaaatca agacaaggag ttaaatcaga atgaatagag
     8461 agatgttgta tttaaataga tcagatattg aacaagcggg aggtaatcat tcacaagttt
     8521 atgtggacgc attaacagaa gcattaacag cccatgcgca caatgatttt gtacaaccgc
     8581 ttaagccgta tttaagacag gatcctgaaa atggacacat cgcagatcga attattgcaa
     8641 tgccaagtca tatcggtggt gaacacgcaa tttcaggtat taagtggata ggtagtaagc
     8701 acgacaatcc atcgaaacgt aatatggagc gtgcaagtgg tgtcattatt ttgaatgatc
     8761 cagaaacgaa ttatccaatt gcagttatgg aagcaagttt aattagtagt atgcgtactg
     8821 cagcagtttc agtgattgca gctaagcatt tggctaaaaa aggatttaaa gacttaacaa
     8881 tcattggatg cgggctaatc ggagacaagc aattacaaag tatgttagag caattcgatc
     8941 atattgaacg cgtgtttgtt tacgatcaat tctctgaagc atgtgcacgc tttgttgata
     9001 gatggcaaca acaacgtccg gaaattaatt ttattgcgac agaaaatgct aaagaagcag
     9061 tatcaaatgg tgaagtagtc attacatgta ccgtaacgga tcaaccatac attgaatatg
     9121 attggttaca aaagggtgca tttattagca acatttctat catggatgtg cataaagaag
     9181 tctttattaa agctgacaaa gtcgtagtag atgactggtc acaatgtaat cgagaaaaga
     9241 aaactattaa ccaattggtg ttagaaggta aattcagcaa agaagcactt catgctgaac
     9301 taggacaact tgtgacaggt gacataccag gacgtgaaga cgatgatgaa atcatattac
     9361 ttaatccgat gggtatggct atcgaagata tttcaagtgc ttactttatt tatcaacagg
     9421 cacaacaaca aaatattggg acaacattga acctatatta agaatgcgag gtgtctgaac
     9481 attgcagaat catacagcag tcaatacagc acaaacgata atattaaggg atttagttga
     9541 tgcattatta tttgaagata tagcctgtat tgtatcgaat agtgagatta ctaaagaaaa
     9601 tggacaaacc attttgatat acaaacgtga aacacaacaa ataaagatac ctgtttattt
     9661 tagtgcttta aatatgtttc gttatgaaag ttcacaacca attacgatag aaagaaggac
     9721 gtctaaacaa ccactaactg cagctgaatt ttggcaaaca atcgctaata tgaattgtga
     9781 tttaagtcat gaatgggaag tggctcgcgt tgaagaagga ctgactactg ctgccacaca
     9841 gcttgctaaa caattatcag aattagattt agcgtcgcat ccttttgtga tgtccgaaca
     9901 gtttgcaagt ttaaaagatc gtccatttca tccattagct aaagaaaaaa gaggattaag
     9961 agaagcagat tatcaagtgt atcaagctga attaaatcaa tcatttcctt taatggttgc
    10021 agcagttaaa aagacacata tgattcatgg cgatactgca aatattgatg aattagaaaa
    10081 tttgacagca cctataaaaa gacaagcgac agatatgtta catgataaag ggttatcaat
    10141 agatgactat gtactatttc ctgtacatcc ttggcaatat caacatattt tgccgaacgt
    10201 ctttgcgaaa gagattagtg aaaaattggt tgtactatta ccgttaaaat ttggagatta
    10261 tctatcgtct tcaagtatgc gttcattaat tgatattagc gcaccgtata atcatgttaa
    10321 agtaccattt gcaatgcagt cattaggcgc attaaggcta acgcctacgc gttacatgaa
    10381 aaacggagaa caagcagaac aattattacg tcagcttata gaaaaagatg aagcactagc
    10441 taagtatgtc atggtttgtg atgaaacagc ttggtggtca tatatgggtc aagataatga
    10501 tattttcaaa gatcaattag gtcatttaac tgttcagtta agaaaatatc ccgaagtgct
    10561 agccaaaaat gatacgcaac agctagtgtc aatggcagca ctcgcggcaa atgatcgcac
    10621 tttatatcaa atgatttgtg gaaaagataa tatttctaaa aatgatgtca tgacgttatt
    10681 tgaagatatc gcacgagtct ttttaaaggt aacactatca tttatgcaat acggcgcatt
    10741 accagagttg catggtcaaa atatattgtt gtcatttgaa gatggacgtg tacaaaaatg
    10801 cgtgttacgt gatcatgata ctgtcagaat ttataaacca tggctaacag cacatcaact
    10861 ttcattgccg aagtatgtcg ttagagaaga tacacctaat acgctaatta atgaagattt
    10921 ggaaacattc tttgcttatt ttcaaacatt agctgtatcg gtaaatctat atgccattat
    10981 tgatgcaatt caagatttat ttggtgtaag tgagcatgaa cttatgtcgt tgttaaaaca
    11041 aattttaaaa aatgaagtgg caactatttc ctgggttaca actgatcagc tagctgtcag
    11101 acacatttta tttgataaac agacgtggcc attcaaacaa attttattac cattgctata
    11161 tcaacgtgat agtggtggag gtagtatgcc ttcaggttta actaccgtac caaatccaat
    11221 ggtgacatat gattaatcag tctatatggc gcagtaactt tcgcatttta tggctcagtc
    11281 agtttatagc gattgctgga ctgacagtac ttgtgccatt attgccaatt tatatggcat
    11341 cactacaaaa tctatcagtc gtagaaatac agttgtggag tggtatagcg attgctgctc
    11401 cagctgtaac gacgatgata gcttcgccga tatgggggaa gctaggtgat aagattagcc
    11461 gaaaatggat ggtgttaaga gcgttacttg gtttggcggt atgcttattt ttaatggcat
    11521 ggtgtactac accattacag tttgtacttg tgaggttatt gcagggacta tttggtggtg
    11581 ttgttgatgc atcaagtgcg tttgcgagtg cagaggcgcc ggctgaagat cgtggaaaag
    11641 tattaggaag attacaaagt tcagtcagtg cagggtctct tgtaggacca ttaatcggcg
    11701 gtgttacagc ttcgatatta ggttttagtg cgttactgat gagtattgcc gttattactt
    11761 ttattgtctg tattttcggt gcattaaaat tgattgaaac gacacatatg ccaaaatcac
    11821 aaacaccaaa tattaataaa ggtattcgcc gttcatttca atgtctatta tgcacgcaac
    11881 aaacatgtcg atttattatc gttggcgttt tagcaaactt tgctatgtat ggcatgctaa
    11941 ctgcattatc accacttgct tcatcagtga atcatacagc gatagatgac cgtagtgtga
    12001 ttggattttt acagtccgca ttttggacgg cttcgatatt aagcgcgcct ttatggggtc
    12061 gctttaatga taaatcatat gttaaatcag tatatatatt tgccacgatt gcatgtggtt
    12121 gtagtgcgat actgcaaggt ttagcgacga atatagagtt tttaatggct gcaagaatac
    12181 ttcaaggatt aacatatagt gcattgattc aaagtgtcat gtttgttgtt gtaaatgctt
    12241 gtcatcagca acttaaaggc acatttgttg gaacgacgaa cagtatgtta gttattggtc
    12301 aaattattgg tagtcttagt ggcgctgcca ttacaagtta tactacacca gctactacgt
    12361 ttatcgttat gggcatagta tttgctgtaa gtagtttatt tttaatttgt tcaaccatca
    12421 ctaatcaaat caacgatcac acattaatga aattatggga gttgaaacaa aaaagtgcaa
    12481 aataaagaat taatacaaca tgcagcgtat gcggctatcg aacgcatttt aaatgaatat
    12541 tttagagaag aaaatttata tcaagtacca cctcaaaatc atcaatggtc tatacaatta
    12601 tctgagctcg aaacgttaac gggtgaattt cgctattggt ctgcgatggg gcatcatatg
    12661 tatcatccag aggtatggct tattgatgga aaaagtaaaa aaataacaac ttataaagaa
    12721 gcaattgcgc gtattttaca acatatggct caaagtgcag ataatcaaac ggcagtgcaa
    12781 caacatatgg cgcaaattat gtctgacatc gataacagca ttcatcgcac ggcgcgttat
    12841 ttgcaaagta acacaataga ctacgcagag gatcgctata tcgtttcaga acaatcttta
    12901 tacttaggtc atccatttca tccgactcct aagagtgcaa gtgggttttc agaagcagat
    12961 ttagagaaat atgcacccga atgtcataca tcattccaat tgcattattt agctgtgcat
    13021 caagatgttc tgctcacgcg ctatgtagaa ggtaaagaag atcaggttga gaaagtgttg
    13081 tatcaattag cagatataga catatcagag atacctaaag attttatttt attaccaata
    13141 catccttatc aaatcaatgt gttgcgacag tatccacagt atatgcaata tagtgaacaa
    13201 ggtttaataa aagaccttgg cgtttccggt gattcagtgt acccaacgtc ttcggttaga
    13261 actgtatttt caaaagcatt aaacatttat ttgaaattac cgatacacgt taaaatcact
    13321 aattttatac gtacgaatga ccttgaacag attgaacgga caatcgatgc cgcgcaagtt
    13381 atcgcatcag tcaaagatga ggttgaaaca ccccatttta aattgatgtt tgaagaagga
    13441 tatcgtgcat tgttaccgaa tccattaggg caaacagttg aacctgaaat ggatttatta
    13501 acaaatagtg ccatgattgt tcgtgaaggg ataccaaatt accatgctga taaagatatt
    13561 catgtattgg cgtcattatt tgaaacgatg cctgattcac cgacctctaa gttatcacaa
    13621 gtgattgagc aaagtggttt agcagcagaa gcatggcttg aatgttattt ggatcgtaca
    13681 ttattgccga tattaaagct gtttagtaac acaggcatta gtctagaagc acatgttcaa
    13741 aatacattaa ttgaattaaa agatggcata ccagaagtgt gctatgtcag agatttagaa
    13801 ggtatttgtc tatctagaac gattgctact gaaaaacaac ttgtgccaaa tgttgtggca
    13861 gcatcaagcc ctgttgtata tgcacatgat gaagcatggc atcgtcttaa atattacgtt
    13921 gtagtaaatc acttagggca tttagtatca actattggta aagcgtctag aaatgaagtt
    13981 gtgttatgga aacttgtagc acatcgtctt atgacttgga aaaaagaata cgcgaataac
    14041 gcagtatttg ttgcctgtgt agaagattta tatcaaaagc cgaccattgc ggctaaagcg
    14101 aatttgatga gtaaattgaa tgattgtggt gcaaacccta tttatacaca tataccaaat
    14161 ccaatttgtc ataacaagga ggtatcgtat tgtgaatcaa acaattctta atcgtgtaaa
    14221 gactagagtg atgcaccaac tggtatcctc acttatttat gagaatatcg ttatttataa
    14281 gacgtcatat caagacggtg tcggtcattt tacaatagaa ggacaagatt cagagtatcg
    14341 ttttactgct gaaaagacac atagctttga tcgtatacgt atcacatcac caattgagcg
    14401 tgtcgtagga gatgaggcag atacaacaac agactataca caattactga gagaggttgt
    14461 atttacattt cctaaaaatg atgaaaagct agaacaattt attgtcgagt tattacagac
    14521 agaactaaaa gatacacaaa gtatgcagta tcgagaatca aacccaccag caacacctga
    14581 gacatttaac gactatgaat tttatgcgat ggaaggtcat cagtatcatc caagttacaa
    14641 atcacgttta ggatttacgt tgagtgataa tttgaaattt ggtcctgact ttgtaccaaa
    14701 cgttaaactg caatggttag ctatcgacaa agataaatta gaaacgacgg tatcaagaaa
    14761 tgttgtagtt aacgaaatgt tacgtcaaca agttggcgat aagacttatg aacattttgt
    14821 acagcaaatt gaagcgtctg gcaaacatgt aaatgatgtt gagatgatac ctgtacaccc
    14881 atggcagttt gaacatgtca tccaagttga tttggctgaa gaaaggctta atggcacagt
    14941 actatggtta ggggaaagtg atgagctata tcaccctcaa caatcgattc gtacgatgtc
    15001 gccaatagac acgacaaaat attatttaaa ggtaccaata agtataacga atacttcaac
    15061 gaaacgagtg ttggcgcctc atacaattga aaatgcagcg caaattacgg attggttaaa
    15121 gcagatacag caacaagata cgtatttaaa agatgaatta aagacagctt ttctagggga
    15181 agtcttagga cagtcttatt taaatacaca actgtcgcct tataaacaaa ctcaagttta
    15241 tggtgcgtta ggtgttatat ggcgtgaaaa tatatatcat atgttaatcg atgaagagga
    15301 tgcgatacca tttaatgcac tttatgcgag tgataaggat ggtgtaccat tcattgaaaa
    15361 ttggattaaa caatatggtt ctgaagcttg gacaaagcaa tttttagctg tagcgattcg
    15421 tccaatgatt catatgcttt attatcacgg tattgccttt gaatcgcatg cacaaaatat
    15481 gatgctcatt catgaaaatg gttggcctac acgtattgcc ttaaaagatt tccatgatgg
    15541 tgttcgtttt aagcgtgagc atttaagtga agctgcttca cacctgacat taaagccgat
    15601 gccagaagca cataaaaaag tgaatagtaa ttcatttatt gaaacagatg acgaacgttt
    15661 agtacgagac tttttacatg atgcattttt ctttattaat atcgccgaaa tcatcttatt
    15721 tattgaaaag caatatgata tcgatgagaa gctgcaatgg aaatgggtta aaggcatcat
    15781 cgaggcgtat caagaagcat ttccagagtt gaataactat aaacatttcg atttgtttga
    15841 acctacgatt caagttgaaa agttaacgac acgtcgatta ttaagtgact ccgagttaag
    15901 aattcatcat gttacaaatc cattaggtgt aggaggtatt actgatgcaa caactatctc
    15961 tgaaacatag attaaacaat ggtgattcag tttatggcat ttttaattct ataccggacc
    16021 cattaatgat agaggttatc gcagcaagcg ggtatgactt tgttgtgatt gatacagaac
    16081 acgtggcgat taatgatgag acactagcgc atttaattcg tgcagctgaa gcagcccata
    16141 ttataccaat tgtacgtgtc actgcagtga tagatagaga tatcattaaa gtgttggata
    16201 tgggtgcgag aggtattatt gtgccacacg ttaaagatca tgagacagtt gagcatattg
    16261 tgaaattaag tcgttattac ccgcaaggat taagaagttt gaatggtggt cgcatggcaa
    16321 gatttggacg tacaccatta cttgatgcaa tggagatggc taatgagcat attatggtga
    16381 ttgccatgat agaagatgtt gaaggggtta tggccattga cgatatagca caagtcgaag
    16441 gtttagacat gatagtcgaa ggtgccgcag atttatccca gtcacttggc ataccatggc
    16501 aaacgcgtga tgatcaagta acatcacatg ttcaacatat ttttgaagtt gtgaatgcac
    16561 atggtaaaca tttttgtgca ttaccacgtg aagatgaaga tattgcaaaa tggcaggcac
    16621 aaggtgtaca aacatttatt ttaggtgatg atcgtggaaa aatatatcgc catttaagtg
    16681 catctctagc gacgtctaaa cagaaggggg atgaagacta atgcgtatag ttcaacccgt
    16741 tattgaacaa ttaaaagcac aatctcatcc agtttgtcat tatatctatg atttagtcgg
    16801 actggaacat catttgcaac atattacatc gtcattgcct agtaattgtc agatgtacta
    16861 tgcaatgaaa gcaaatagtg aacgaacaat cctagataca attagtcagt atgttgaagg
    16921 attcgaagtt gcatctcaag gtgaaatagc aaaaggtctt gcttttaaac caacaaatca
    16981 tattattttt ggtggccctg gtaagacaga cgaggaacta agatatgcag taagtgaagg
    17041 tgttcagcgt attcatgttg aaagtatgca tgaattacaa cggctaaatg ccatcttaga
    17101 agatgaagat aagacacaac acattttatt gcgtgttaat ttagcaggac catttcccaa
    17161 tgcaacgttg catatggcag gacgcccaac acaatttggt atttctgaag acgaagttga
    17221 tgaagtcatt gaagctgcgc tagcaatgcc aaagattcat ctagatggat ttcattttca
    17281 ttctatttct aacaatttag actcgaattt acatgtcgat gtagtgaaac tttattttaa
    17341 aaaagcaaag gcatggtctg aaaaatatcg atttccactc aaacatatca atcttggtgg
    17401 tggtataggg gttaactatg cagatttaac tagccaattt gagtgggata attttgtaga
    17461 aaattttaaa acacttatcg ttgagcaaga aatggaagat gtgacattga actttgaatg
    17521 tgggcgcttt attgtggcac atattggtta ctatgtgaca gaagtgctag atattaagaa
    17581 agtgcatgat gcttggtatg ccattttaag aggaggtacg caacaattta gactgccggt
    17641 atcttggcag cataaccatc cttttgaaat ttatcgctat aaggacaatc catattcatt
    17701 tgaaaaagtt tcaatttcga gacaggacac aacgttagtc ggtcaattat gtacaccgaa
    17761 agatgtcttt gctagagaag tacagataga cgcaatcagt acaggcgacg ttattgtttt
    17821 caaatatgca ggtgcatacg gatggtctat ttcacatcac gatttcttaa gccatccaca
    17881 tcctgaattt atttatttaa cgcaaacaaa ggaggatgaa taactattga atcatattca
    17941 tgaacattta aaattggtac cagtagataa gattgatctt cacgaaacat tcgaaccttt
    18001 aagattggaa aagacgaaaa gtagtattga agcagatgat tttatacgtc atcctatttt
    18061 agtgacagcg atgcaacatg gtagatatat ggttatagat ggtgtgcatc ggtatacaag
    18121 tttgaaagcg ttaggatgta agaaagttcc agtgcaagaa atccatgaaa cacaatattc
    18181 aattagtaca tggcaacata aagttccatt tggtgtgtgg tgggaaacgt tacaacaaga
    18241 acatcgcttg ccatggacta ctgagacaag acaagaagcg ccatttatta cgatgtgtca
    18301 tggtgataca gaacaatatt tgtatacaaa agatttaggc gaagcacatt ttcaagtatg
    18361 ggaaaaggtt gtcgcaagtt atagtggttg ttgttctgta gagagaattg cacaaggtac
    18421 atatccttgt ctttctcaac aagatgtact catgaagtat cagccattga gttataagga
    18481 aattgaagcg gttgttcata aaggggaaac tgtgccagca ggtgtgacac gctttaatat
    18541 ttcaggacga tgtcttaatc ttcaagtacc actggcatta cttaaacaag atgatgatgt
    18601 tgaacaactg cgcaattgga agcagttttt agcagataag tttgccaata tgagatgcta
    18661 tactgaaaaa gtatacttgg tggagcaata gttttactgt gatgttgagg gaaatatgat
    18721 gatttagcgt attgatagcg aaaatataat aaaacaatat agtgtggaga acttttgata
    18781 ttttataaat attgaagttc tccatttttg tattttgcat ataaaaatta aataaaataa
    18841 ggtatattaa ggtaaagtat aaatattaaa taaatgggga atagtcatga acatgaattt
    18901 aattatagga aaaatcgcta tttggatagg catcgtagtt caaatatttt atagcgtcgt
    18961 tattgttagt ataacaacct tcatcctttt tataaatgct aaaggagcaa gcccggaaat
    19021 agctgtttta atagcattag taatcattct tatcttaact ttaccaccta tcctaaccgc
    19081 gattaactta gagaataaaa tggaagtaaa aggtacgctt tttattgttt atgcgattgt
    19141 tgcattttgt atgtttaatt acctttcatc aattttatgg gtggtttgcg gcatttttct
    19201 tatttggact aaatattcaa aaggtgaatc gacagacgat gatgagaatg aaaaagttga
    19261 tattgaaagt acagagaatc aatttgattc taaagataaa atcactaaag aataaagtga
    19321 atatttaagg cggagtataa atattaaata aacggggaat gagcatgagg aaaaatatag
    19381 aattgatatt aatatggatg ggtatatata ttcaaatagc atacatggct attatagctt
    19441 tgctcatata tatatttaca ttattttttg gatcattttt tggattttta tttttgttaa
    19501 caggtaatga aattggagat ttattaaaaa cacccacttc atttatgata tttgctacat
    19561 gtctaattat attttcagtc ttacctgtta ctgtactagc atttgactta tctaaaagaa
    19621 gaattttaaa aggtatcata ttagttgtat atgcgattat tgctgcgtta ttatacaact
    19681 ttgtatctgc aatactttgg tttattgtag ccatttctct ttttattaga aaaaaatctt
    19741 tatctgcaga aaatactgtt gctgttcaaa gtcacaaagg agcttctaaa caagaaatac
    19801 gttataaact tgtgtacaag tctgtctcga taaaacatag tgatgattca attaaagtag
    19861 cacatacagt gggaaataaa aaactaattg atgacaaaac aattgatatt caccttgatt
    19921 caagtgaaat attaaaaaag attagtactg acgcaaatca agcatcacat aaagacacgt
    19981 gtaaaaagga acttgatagt caagacatga tggaacatcc tgagtttaaa aatcccacga
    20041 ctaaaaacct tgaaggatta aacgaagaaa tacataaaga tgaagctaca actaaagttg
    20101 acagtgataa cacggaaccg ccgattgaat caaaagacca tgtctcgaaa aaagattgat
    20161 gacaaactaa tcgagagact taaaaaaata atattcaaca taagcacttt taaaacgaca
    20221 tttaaacgca ttgctaatca ctaatggtag tgcgtttaac tataccttaa atatctgaat
    20281 attttgttaa atggagttac ctttgttgta ctattcaaat gaagaggagt aaaatgtaat
    20341 taaaggaaag taatttgagg agtgatcttt atgacaaaca acaaagtagc attagtaact
    20401 ggcggagcac aagggattgg ttttaaaatt gcagaacgtt tagtggaaga tggtttcaaa
    20461 gtagcagttg ttgatttcaa tgaagaaggg gcaaaagcag ctgcacttaa attatcaagt
    20521 gatggtacaa aagctattgc tatcaaagca gacgtatcaa accgtgatga tgtatttaat
    20581 gcagtaagac aaactgcagc gcaatttggc gatttccatg ttatggttaa caatgccggc
    20641 cttggaccaa caacaccaat cgatacaatt actgaagaac agtttaaaac agtatatggc
    20701 gtgaacgttg caggtgtgct atggggtatt caagccgcac atgaacaatt taaaaaattc
    20761 aatcatggcg gtaaaattat caatgcaaca tctcaagcag gcgttgaggg taacccaggc
    20821 ttgtctttat attgcagtac aaaattcgca gtgcgaggtt taacacaagt agccgcacaa
    20881 gatttagcgt ctgaaggtat tactgtgaat gccttcgcac ctggtatcgt tcaaacacca
    20941 atgatggaaa gtatcgcagt ggcaacagcc gaagaagcag gtaaacctga agcatggggc
    21001 tgggaacaat ttacaagtca gattgctttg ggcagagttt ctcaaccaga agatgtttca
    21061 aatgtagtga gcttcttagc tggtaaagac tctgattaca ttactggaca aacaattatt
    21121 gtagatggtg gtatgagatt ccgttaataa tcatccacta atgataaata aatccttatt
    21181 gttaagttta atcacttagc agtaaggatt ttttagtgca cttagaaggg attgtattgg
    21241 tagaaaatta ataagcgaag ttcttaagtg agttatgatg tcactgtcta atgcatcagt
    21301 tgaaagcatt actagtatta acacaccaaa gatattataa aacatcacaa aaaccccact
    21361 atctaattta tctcattaaa aattcacaaa gttatctcat tttattttta taaataaaaa
    21421 atatcgataa aaagcttaca atactttatg tttttatgat atatttttaa tgtataaaag
    21481 aggtggaaga tttggaaaga gttttgataa ctggtggggc tggttttatt gggtcgcatt
    21541 tagtagatga tttacaacaa gattatgatg tttatgttct agataactat agaacaggta
    21601 aacgagaaaa tattaaaagt ttggctgacg atcatgtgtt tgaattagat attcgtgaat
    21661 atgatgcagt tgaacaaatc atgaagacat atcaatttga ttatgttatt catttagcag
    21721 cattagttag tgttgctgag tcggttgaga aacctatctt atctcaagaa ataaacgtcg
    21781 tagcaacatt aagattgtta gaaatcatta aaaaatataa tagtcatata aaacgtttta
    21841 tctttgcttc gtcagcagct gtttatggtg atcttcctga tttacctaaa agtgatcaat
    21901 cattaatctt accattatca ccatatgcaa tagataaata ttacggcgaa cggacgacat
    21961 taaattattg ctcgttatat aacataccaa cagcggttgt taaatttttt aatgtatttg
    22021 ggccaagaca ggaccctaag tcacaatatt caggtgtgat ttcaaagatg ttcgattcat
    22081 ttgagcataa caagccattt acattttttg gtgacggact gcaaactaga gattttgtat
    22141 atgtatatga tgttgttcaa tctgtacgct taattatgga acacaaagat gcaattggac
    22201 acggttataa cattggtaca ggcactttta ctaatttatt agaggtttat cgtattattg
    22261 gtgaattata tggaaaatca gtcgagcatg aatttaaaga agcacgaaaa ggagatatta
    22321 agcattctta tgcagatatt tctaacttaa aggcattagg atttgttcct aaatatacag
    22381 tagaaacagg tttaaaggat tactttaatt ttgaggtaga taatattgaa gaagttacag
    22441 ctaaagaagt ggaaatgtca taaaaataac attgaagatg ttcatataaa taaaaattat
    22501 aattatcaaa gaaaattaga tgcactcgaa gaagtgagaa aaggctatta cccaattaaa
    22561 cgtgcgattg acttagtatt aagtatcgtt ttattatgtt taacatttcc gattatgttc
    22621 atattcgcca ttgctattgt catagattcg ccaggaaatc ctatttatag tcaggttaga
    22681 gttgggaaga tgggtaaatt aattaaaata tacaaattac gttcgatgtg taaaaacgca
    22741 gagaaaaatg gtgcgcaatg ggctgataaa gatgatgatc gtataacaaa tgtcgggaag
    22801 tttattcgta aaacacgcat tgatgaatta ccacaactaa ttaatgttgt taaaggggaa
    22861 atgagtttta ttggaccacg cccggaacgt ccggaatttg tagaattatt tagttcagaa
    22921 gtgataggtt tcgagcaaag atgtcttgtt acaccagggt taacaggact tgcgcaaatt
    22981 caaggtggat atgacttaac accgcaacaa aaactgaaat atgacatgaa atatatacat
    23041 aaaggtagtt taatgatgga actatatata tcaattagaa cattgatggt tgttattaca
    23101 ggggaaggct caaggtagtc ttaatttact taataagttc aaataaaagt tacattttaa
    23161 agattgtgac caattgttac agtataacga ggaatccctt gagacagtat caaatggcat
    23221 taagaaatat gtgccatcat tgatttgcat ggctataaat actattcatc tgatgagata
    23281 gctatgttaa gaaattgaaa gtatagcgtt aaaggggttt gtaacagttg aaaattatat
    23341 attgtattac taaagcagac aatggtggtg cacaaacaca tctcattcaa ctcgccaacc
    23401 atttttgcgt acaccatgat gtttatgtca ttgtaggcaa tcatggacca atgattgaac
    23461 aactagatgc aagagttaat gtaattatta tcgaacattt agtaggtcca attgacttta
    23521 aacaagatat tttagctgtc aaagtgttag cacagttatt ctcgaaaatt aaacctgatg
    23581 ttatccattt acattcttcc aaagctggaa cggtcggacg aattgcgaag ttcatttcga
    23641 aatcgaaaga cacacgtgta gtttttacag cgcatggatg ggcttttaca gagggtgtta
    23701 aaccagctaa aaaatttcta tatctagtta tcgaaaaatt aatgtcacgt attacagata
    23761 gcattatttg tgtttcagat ttcgataaac agttagcact aaaatatcga tttaatcgat
    23821 tgaaattaac cacaatacat aatggtattg cagatgttcc cgctgttaag caaacgctaa
    23881 aaagccaatc acataacaat attggcgaag tagttggaat gttgcctaat aaacaagatt
    23941 tacagattaa tgccccgaca aagcatcaat ttgttatgat tgcaagattt gcttatccaa
    24001 aattgccaca aaatctaatc gcggcaatag agatattgaa attacataac agtaatcatg
    24061 cgcattttac atttataggc gatggaccta cattaaatga ttgtcagcaa caagttgtac
    24121 aagctgggtt agaaaatgat gtcacatttt tgggaaatgt cattaatgcg agtcatttat
    24181 tatcacaata cgatacgttt attttaataa gtaagcatga aggtttgcca attagcatta
    24241 tcgaagctat ggctacaggt ttgcctgtta tagccagtca tgttggcggt atttcagaat
    24301 tagtaactga taatggtata tgtatgatga acaaccaacc cgaaactatt gccaaagtcc
    24361 tggaaaaata tttaatagac agtgattaca tcaaaatgag taatcaatct agaaaacgtt
    24421 atttagaatg ttttactgag gagaaaatga ttaaagaagt ggaagacgtt tataatggaa
    24481 aatcaacaca atagtaaatt actaacattg ttacttatcg gtttagcggt ttttattcag
    24541 caatcttcgg ttattgccgg tgtgaatgtt tctatagctg actttatcac attactaata
    24601 ttagtttatt tactgttttt cgctaaccat ttattaaagg caaatcattt tttacagttt
    24661 ttcattattt tgtatacata tcgtatgatt attacgcttt gtttactatt ttttgatgat
    24721 ttgatattta ttacggttaa ggaagttctt gcatctacag ttaaatatgc atttgtagtc
    24781 atttatttct atttagggat gatcatcttt aagttaggta atagcaagaa agtgatcgtt
    24841 acctcttata ttataagcag tgtgactata ggtttatttt gtattatagc cggtttgaac
    24901 aagtcccctt tactaatgaa attgttatac tttgatgaaa tacgttcaaa aggattaatg
    24961 aatgacccta actatttcgc gatgacacag attatcacat tagtacttgc ttacaagtat
    25021 gttcataatt atgtatttaa agtgattgca tgtggcattc tgctatggtc attaactaca
    25081 accggatcta agactgcgtt tatcatatta atcgtcttag ccatttattt ctttattaaa
    25141 aagttattta gcagaaatgc ggtaagtgtt gtgagtatgt tagtgattat gctgatatta
    25201 ctttgtttta ccttttataa tatcaactac tatttattcc aattgagcga tctagatggc
    25261 ttaccatctt tagatcgaat ggcatcaatt tttgaagagg gatttgcatc attaaatgat
    25321 agcggatctg agcgaagtgt tgtatggata aatgccattt cagtaattaa atatacacta
    25381 ggttttggtg tcggattagt ggattatgta catattggct cgcaaattaa tggtatttta
    25441 cttgttgccc ataatacata tttgcaaatt tttgcggaat ggggcatttt attcggtgcg
    25501 ttatttatca tatttatgct ttatttactg tttgaattat ttagatttaa catttctggg
    25561 aaaaatgtaa cagcaattgt tgtaatgttg acgatgctga tttacttttt aacagtatcg
    25621 tttaataact caagatatgt cgcttttatt ttaggaatta tcgtctttat tgttcaatat
    25681 gaaaagatgg aaagggatcg taatgaagag tgattcacta aaagaaaata ttatttatca
    25741 agggctatac caattgatta ggacgatgac accactgatt acaataccca ttatttcacg
    25801 tgcatttggt cccagtggtg taggtattgt ttcattttct ttcaatatcg tgcaatactt
    25861 tttgatgatt gcaagtgttg gcgttcagtt atattttaat agagttatcg cgaagtccgt
    25921 taacgacaaa cggcaattgt ctcagcagtt ttgggatatc tttgttagta aattattatt
    25981 gtcgctaaca gtttttgcga tgtatatcgc tgtgattaca atctttatag atgattacta
    26041 ccttattttc ctactacaag gaatctatat tataggtgca gcactcgata tttcatggtt
    26101 ttatgctgga actgaaaagt ttaaaatacc tagccttagt aatattgttg cgtctgttat
    26161 tgtattaagt gtcgttgtta tctttgtcaa agatcaatca gatttatcat tgtatgtatt
    26221 tactattgct attgtgacgg tattaaacca attacctttg tttatctatt taaaacgata
    26281 cattagcttt atttcagtta attggataca cgtctggcaa ttgtttcgtt cgtcattagc
    26341 atacttatta ccaaatggac agctcaactt atatactagt atttcttgcg ttgttcttgg
    26401 tttagtaggc acataccaac aagttggtat cttttctaat gcatttaata ttttaacggt
    26461 cgcaatcata atgattaata catttgatct tgtaatgatt ccgcgtatta ccaaaatgtc
    26521 tatccagaaa tcacatagtt taactaaaac gttagctaat aatatgaata ttcaattgat
    26581 attaacagta cctatggtct ttggtttaat tgcaattatg ccatcatttt atttatggtt
    26641 ctttggtgag gaattcgcat caactgtccc attgatgact attttagcga tacttgtatt
    26701 aatcattcct ttaaatatgt tgataagcag gcaatattta ttaatagtga ataaaataag
    26761 attatataat gcgtcaatta ctattggtgc ggtaatgaat ttagtattat gtcttgtttt
    26821 gatatatttt tatggaattt acggtgctgc tattgcgcgt ttaattacag agtttttctt
    26881 gctcatttgg cgatttattg atattactaa aatcaatgtg aagttgaata ttgtaagtac
    26941 gattcaatgt gtcattgctg ccgttatgat gtttattgtg cttggtgtgg tcaatcatta
    27001 tttgccccct acaatgtacg ctacgctgct attaattgcg attggtatag tagtttatct
    27061 tttattaatg atgactatga aaaatcaata cgtatggcaa atattgaggc atcttcgaca
    27121 taaaacaatt taagtatcgg taacgctata ctttagaaat ttaagatgaa gaagaaaagg
    27181 caatttctta ttgaaaaatg gaagttgtct tttttaattc tttttaaaag cgggaaacaa
    27241 aagcagttaa atgccttttt gcattcaata ttaaatatta tatcaatttc gaatatttaa
    27301 atttcatata attgaatata acaaataaat aataattatt gcaaaacaca cccgaaatta
    27361 attattataa aagtatattc ataaaaggag gaatatactt atggcattta aattaccaaa
    27421 tttaccatat gcatatgatg cattggaacc atatatagat caaagaacaa tggagtttca
    27481 tcacgacaaa catcacaata cgtacgtgac gaaattaaac gcaacagttg aaggaacaga
    27541 gttagagcat caatcactag cggatatgat tgctaactta gacaaggtac cggaagcgat
    27601 gaggatgtca gtccgtaata atggcggtgg tcattttaac cattcattat tctgggaaat
    27661 actatcacct aattctgaag aaaaaggtgg cgtaatagac gacattaaag cgcagtgggg
    27721 cactttagat gaatttaaaa atgaatttgc aaataaagca acaacattat ttggatcagg
    27781 ttggacttgg ttagttgtta atgatggaaa attagaaatt gtgacaacgc caaaccaaga
    27841 taatccatta acagaaggca aaacaccaat cttactattt gatgtttggg agcatgccta
    27901 ctatctgaaa tatcaaaata aacgtccaga ctatatgact gcattttgga atattgttaa
    27961 ctggaaaaaa gttgatgaat tataccaagc agcaaaataa tataacttaa tataattgag
    28021 gtggagcatc tacaaggtgt ttcacccttt tttgtactta atatctttac ttattcgttt
    28081 ttgtgtaatg ggatagcacg taaaagtggc aaaataataa gataaatttc aactcgaaat
    28141 accttattat tgttgactat aacgataatc taaatgctat atacatcaga caacactatt
    28201 gattaatcaa catattgata ttaaataaat tgacaaaata agtaattatt gtgaatctaa
    28261 agtgaaattt ttataaaaaa atgtaatgat tcaaaaattt tgttgcattt cttttgtaat
    28321 catatgataa tgtaaatgta atcaaattgt aatataaagg gaaaagacaa tgaaaaaatt
    28381 agcaacagta ggttctttaa ttgtaacaag cacttttgta ttctcaagta tgccttttca
    28441 aaatgcgcat gctgacacaa cttcaatgaa tgtgtcgaat aaacaaagcc aaaatgtaca
    28501 aaatcatcgc ccttatggcg gagtagtacc acaaggaatg acgcaagcac aatatactga
    28561 attagagaaa gctttacccc aattaagcgc tggcagtaat atgcaagact ataatatgaa
    28621 attgtatgat gcgacgcaaa atattgctga taaatacaat gtgataatta caactaatgt
    28681 aggggtattt aaaccacatg ctgttagaga tatgaatgga catgcgttac ctttaacaaa
    28741 agatggcaat ttttatcaaa cgaatgtaga tgcaaatggt gttaatcatg gtggtagtga
    28801 aatggtgcaa aataaaacag gtcatatgag tcaacaaggt catatgaatc agaacacaca
    28861 catgaaccaa cagccacaca tgcaacaagg tcatatgcaa tcatcaaacc atcaaatgat
    28921 gagtccaaaa gcaaatatgc attcatcaaa tcatcaaatg aaccaaagta acaaaaaagt
    28981 tttaccagct gctggtgaaa gtatgacatc aagtattctt actgcaagta ttgccgcact
    29041 actattagta tctgggttat tcttagcatt tagacgacgt tcaacaaata aataaacata
    29101 atacgattaa taatagaaaa atcgtgtgat tatctgagcg agcctaggac ataaatcaat
    29161 gtcctaggct cgctaatgtt atattggcag tagttgactg aatgaaattg cgcttgtaac
    29221 aagcttttcc atttcttgcc cacttcctaa ataatcatca caaaatttct tatatttcaa
    29281 taattttaat aatccgattg tcttatacgt gtcagtgtta attcagagat ttcctgtgga
    29341 atataccact tattaatcat aattggataa ggtgtttgtg cgtacaatgt ttcaataatc
    29401 agccaacaat gtgtatcacc atcaaacacg tgactatgat ttttgaagtg gggcgctttg
    29461 gtaatagaca tttttaaatc tgattgatat gcattgctat aaatcgtttg ctcaacgaac
    29521 gtcttcatgt cgtcttcgtt ttgtgtattc actttaaatg tgtcaatgac atttaacggt
    29581 ataaaggtaa agcaaaatgc atcagcttgc ttagaatgat tgtccttttt ttgataatag
    29641 cgttccattg caatgacggc agaaggatgg tttgcaaaca aatgatttgt atattcactt
    29701 tctaaatcaa cacgataatt aattgatgac atagatacgc gagctagcaa tatttgatca
    29761 agtggatgct taaattgatc catacttgaa gcgtgttggg catttgtttg tggaataaca
    29821 aagtgtccct tccctcttgt actctctacg atgccatctt cggctaacaa ttttatagct
    29881 tggcgcaaag tcatacgact gacatcaaag cgcgcacaaa gttccttttc agtaggtaat
    29941 gcatggccac tgggatattt tcctatttga atttctttat ataacgtatt ataaatcgtt
    30001 aaaaattttg gttgtgtttg cgtcacgtag acaacctcca taaagttact taatcactct
    30061 catcatacaa taatttttac tcgaattgga aaaattataa aaattaaaca tagatagact
    30121 ttgaaaatta gttttataca aggttagtag ctataactgt aaaatgttct taatattgtc
    30181 aaaatgtaat gcttgaaagc gcttttaaaa aatattatta tatacatggt tagacaaata
    30241 gacaaatcac tatacaaata ttgggaggaa tattttatga aatcaacacc acacattaaa
    30301 ccaatgaatg acgtcgaaat tgcagaaacg gttctattgc caggagatcc gttaagagct
    30361 aagttcattg cagaaacata tttggacgat gtggaacagt tcaatacagt gcgaaacatg
    30421 tttggtttta ccggaacata taaaggtaaa aaagtttctg tcatgggttc aggaatgggt
    30481 atgccatcaa ttggcattta ctcttatgaa ttaattcata catttggttg taaaaaatta
    30541 attcgcgttg gttcttgtgg cgcgatgcaa gaaaacattg atttatatga tgtgattatt
    30601 gcacaaggtg cctctactga ttcaaattac gttcaacaat atcaattacc aggtcatttt
    30661 gcgccaattg cttcttatca gttattagaa aaagcagttg aaacagcacg tgacaaaggt
    30721 gtacgtcatc atgtaggtaa tgtgttatca agtgatattt tctataacgc ggatacaaca
    30781 gcgagtgaac gttggatgcg tatgggtatt ttaggtgtag aaatggaatc agccgcatta
    30841 tacatgaatg caatttacgc tggtgtcgaa gcattaggcg tgttcacagt gagcgatcat
    30901 ttaattcatg aaacgtcaac aacacctgag gaaagggaac gtgcatttac agatatgatt
    30961 gaaattgcac tgtcattggt gtagatgatt atgaatgttg aatattctaa aataaagaaa
    31021 gcagtaccta ttttattatt cttatttgta ttcagtttgg ttatagacaa ctcatttaaa
    31081 ttgatttctg tagccattgc tgatgactta aacatttctg taacgacagt aagttggcaa
    31141 gcgacattag ccggtttagt aattggtatt ggcgctgtag tatacgcttc tttatctgat
    31201 gccattagta tacgcacact atttatttat ggcgtgatat taatcattat tggatcaatt
    31261 attggttaca ttttccaaca tcaattcgca ttacttttag ttggacgtat tattcaaact
    31321 gccggtttag ctgctgcaga gacattatat gtgatatatg ttgcaaagta tctttctaaa
    31381 gaggaccaga agacttacct tggcttaagt acgagcagtt attccttgtc attagttatc
    31441 ggtacattat caggtggatt tatttctacg tatttacact ggacaaatat gtttttaatt
    31501 gcattaatcg tagtatttac gttgccattc ctatttaaat tattaccaaa agaaaataat
    31561 acaaataaag ctcatttaga ttttgttggc ttaattctag tggcaactat cgctacaaca
    31621 gtcatgctgt ttattacgaa ctttaattgg ttatatatga ttggtgcctt aattgcgatt
    31681 atcgtttttg cgctatatat taaaaatgct caacgtccat tagtaaacaa atcatttttc
    31741 caaaataaac gttatgcttc atttttattt atagtattcg taatgtatgc tatccaattg
    31801 ggttatattt ttacgttccc attcataatg gaacaaattt atcatcttca gctagacaca
    31861 acatcactgt tattagtacc gggttatatt gtagcagtca tcgttggtgc actaagtggt
    31921 aaaatcggcg aatatcttaa ttcaaaacaa gcgattatca cagcaattat tttaatcgca
    31981 ctgagcttga ttttacctgc atttgcagta ggtaatcaca tttcaatctt cgtcatttct
    32041 atgatattct ttgcaggtag ctttgcttta atgtatgcac ctttacttaa cgaagccatt
    32101 aaaacaatag attttaatat gacaggtgtg gctattggct tttataattt aattattaat
    32161 gtggcggtat ctgtaggtat tgcgattgct gcggctctaa tcgattttaa agcattaaat
    32221 ttcccaggca atgatgcatt aagttcacat ttcggtatta ttttaattat tttaggttta
    32281 atgagtattg tcggattagt tttattcgtc atcttaaatc gttggacaca atctgaaaaa
    32341 taaatagata taaattcgcg agatatattg gtatttatag taaaattaaa taaagagatt
    32401 atataacacg aggagtagta agtatgaaat ttgagaaata tatagatcac acttta
//
LOCUS       DAHHRR010000019        32405 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.019, whole genome shotgun sequence.
ACCESSION   DAHHRR010000019 DAHHRR010000000
VERSION     DAHHRR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32405
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.019"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..357)
                     /locus_tag="NBI42_000722"
     CDS             complement(<1..357)
                     /locus_tag="NBI42_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD1777162.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQK"
     gene            complement(354..524)
                     /gene="mazE"
                     /locus_tag="NBI42_000723"
     CDS             complement(354..524)
                     /gene="mazE"
                     /locus_tag="NBI42_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCD1777163.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(609..1757)
                     /gene="alr"
                     /locus_tag="NBI42_000724"
     CDS             complement(609..1757)
                     /gene="alr"
                     /locus_tag="NBI42_000724"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCD1777164.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVVGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVAAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(1823..2182)
                     /locus_tag="NBI42_000725"
     CDS             complement(1823..2182)
                     /locus_tag="NBI42_000725"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCD1777165.1"
                     /translation="MIHGIGVDLIEIDRIKVLYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQIVLEKSAF"
     gene            complement(2186..2677)
                     /locus_tag="NBI42_000726"
     CDS             complement(2186..2677)
                     /locus_tag="NBI42_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCD1777166.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNYISIVSSVIIILLVVVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            complement(2664..4247)
                     /locus_tag="NBI42_000727"
     CDS             complement(2664..4247)
                     /locus_tag="NBI42_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCD1777167.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFVIFQLKDFDYTNPE
                     SYLWIGIVFVFFLLSYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEMAQKSST
                     EQNGNRNQDIEGNVEDIAEGRLDEVQNSDSKLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSVSVLIIILLVLILAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVSILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPFSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITVVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            complement(4240..4719)
                     /locus_tag="NBI42_000728"
     CDS             complement(4240..4719)
                     /locus_tag="NBI42_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777168.1"
                     /translation="MSVYTKLSKSPKSALLYYYITNGIEFILSVAVYVIFYFIWLRFE
                     WPNYLIYILLVLGALSTLKLFIKPLWQYHCRFYQIDQLSVQYRTSFLIYKEETSRIER
                     LQYLSIKSNPISKALNLYKVGFMTAGHTIYLPMMSHDDVKIIEARTMSNLRGVESDV"
     gene            complement(4928..5488)
                     /locus_tag="NBI42_000729"
     CDS             complement(4928..5488)
                     /locus_tag="NBI42_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCD1777169.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RVKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHISTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(5508..7535)
                     /gene="kdpB"
                     /locus_tag="NBI42_000730"
     CDS             complement(5508..7535)
                     /gene="kdpB"
                     /locus_tag="NBI42_000730"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCD1777170.1"
                     /translation="MHHVNKYYNQTMVIEALKMSFYKLNPKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMWEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFAELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQADIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIAPFLGIKLIDMIVQFFV"
     gene            complement(7554..9230)
                     /gene="kdpA"
                     /locus_tag="NBI42_000731"
     CDS             complement(7554..9230)
                     /gene="kdpA"
                     /locus_tag="NBI42_000731"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCD1777171.1"
                     /translation="MEIILFLTMMVMIAYVFSGYLYRLALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESGVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLNANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWTEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPLGGLGPMVLMMLNVVFGGEGVGLMNLLIYVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(9254..9364)
                     /locus_tag="NBI42_000732"
     CDS             complement(9254..9364)
                     /locus_tag="NBI42_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HCD1777172.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIGSEKF"
     gene            9502..12149
                     /locus_tag="NBI42_000733"
                     /pseudo
     CDS             9502..12149
                     /locus_tag="NBI42_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
     gene            12149..12844
                     /locus_tag="NBI42_000734"
     CDS             12149..12844
                     /locus_tag="NBI42_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1777173.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTAENATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(13196..14716)
                     /locus_tag="NBI42_000735"
     CDS             complement(13196..14716)
                     /locus_tag="NBI42_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1777174.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDGKSKRSKGYSSKKKGNKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(15233..16591)
                     /locus_tag="NBI42_000736"
     CDS             complement(15233..16591)
                     /locus_tag="NBI42_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCD1777175.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPVIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNTLVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLTNDLKAHDCVLVKGSRGMKLEEVVNAL
                     IS"
     gene            complement(16606..17676)
                     /locus_tag="NBI42_000737"
     CDS             complement(16606..17676)
                     /locus_tag="NBI42_000737"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCD1777176.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            17994..19196
                     /locus_tag="NBI42_000738"
     CDS             17994..19196
                     /locus_tag="NBI42_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCD1777177.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            complement(19460..19597)
                     /locus_tag="NBI42_000739"
     CDS             complement(19460..19597)
                     /locus_tag="NBI42_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777178.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(19725..19934)
                     /locus_tag="NBI42_000740"
     CDS             complement(19725..19934)
                     /locus_tag="NBI42_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCD1777179.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            complement(19946..20239)
                     /gene="csoR"
                     /locus_tag="NBI42_000741"
     CDS             complement(19946..20239)
                     /gene="csoR"
                     /locus_tag="NBI42_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HCD1777180.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            20405..21889
                     /gene="cls"
                     /locus_tag="NBI42_000742"
     CDS             20405..21889
                     /gene="cls"
                     /locus_tag="NBI42_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCD1777181.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRDKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            21917..22564
                     /locus_tag="NBI42_000743"
     CDS             21917..22564
                     /locus_tag="NBI42_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1777182.1"
                     /translation="MQQSDIISAAKKYMKSIHHNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSIEDQEHILFIINNMSY
                     RNGKNDHVTLTLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEQMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNYHD"
     gene            complement(23313..24185)
                     /gene="yidC"
                     /locus_tag="NBI42_000744"
     CDS             complement(23313..24185)
                     /gene="yidC"
                     /locus_tag="NBI42_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCD1777183.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(24271..24912)
                     /locus_tag="NBI42_000745"
     CDS             complement(24271..24912)
                     /locus_tag="NBI42_000745"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCD1777184.1"
                     /translation="MFNQSYLNVYFICGISDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHTPVGPEMIATFKEM
                     NPQLPIVAIGGINTSNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            complement(24914..25705)
                     /gene="thiM"
                     /locus_tag="NBI42_000746"
     CDS             complement(24914..25705)
                     /gene="thiM"
                     /locus_tag="NBI42_000746"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCD1777185.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYAIYKTAIVIT
                     GKEDVIVQGDKAIVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            complement(25689..26519)
                     /gene="thiD"
                     /locus_tag="NBI42_000747"
     CDS             complement(25689..26519)
                     /gene="thiD"
                     /locus_tag="NBI42_000747"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCD1777186.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMSVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSNGVIIKGGHSNDTDIAKDYLFTKEGVQTFENKRFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            complement(26512..27201)
                     /gene="tenA"
                     /locus_tag="NBI42_000748"
     CDS             complement(26512..27201)
                     /gene="tenA"
                     /locus_tag="NBI42_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HCD1777187.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLLGNIQADALRHYLQAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKLSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     regulatory      complement(27281..27383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(27726..28421)
                     /gene="sceD"
                     /locus_tag="NBI42_000749"
     CDS             complement(27726..28421)
                     /gene="sceD"
                     /locus_tag="NBI42_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HCD1777188.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGANGADMSVQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKASEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     gene            complement(28809..29204)
                     /locus_tag="NBI42_000750"
     CDS             complement(28809..29204)
                     /locus_tag="NBI42_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1777189.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSILDIDSQNIDNHDLLEI"
     gene            29398..29838
                     /locus_tag="NBI42_000751"
     CDS             29398..29838
                     /locus_tag="NBI42_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HCD1777190.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDSEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            complement(29895..30335)
                     /gene="fabZ"
                     /locus_tag="NBI42_000752"
     CDS             complement(29895..30335)
                     /gene="fabZ"
                     /locus_tag="NBI42_000752"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCD1777191.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(30369..31634)
                     /gene="murA"
                     /locus_tag="NBI42_000753"
     CDS             complement(30369..31634)
                     /gene="murA"
                     /locus_tag="NBI42_000753"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCD1777192.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGDLQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELNHLDRGYVDLHGKLKQLGADIERIND"
     gene            complement(31745..31978)
                     /locus_tag="NBI42_000754"
     CDS             complement(31745..31978)
                     /locus_tag="NBI42_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCD1777193.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            complement(32110..32202)
                     /locus_tag="NBI42_000755"
     CDS             complement(32110..32202)
                     /locus_tag="NBI42_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777194.1"
                     /translation="MLKSKFPSPLSAVDISFFESSYIFSCQDTL"
BASE COUNT        10388 a         5914 c         4825 g        11278 t
ORIGIN      
        1 tttctgatga gctactgcat tcagccctaa actaatcatt agtgcattat ctacttcttt
       61 cattttatca tcggataagt acgtcagttt ttctttcaat cgttttttat caagtgtacg
      121 aatttgttct aataatataa ctgagtcttt atccaattta tactttttct tttcaatctc
      181 tacatgtgtc ggtattttcg ctttattaat cctaccagtt attgccgcaa caataactgt
      241 aggactatat ttattaccag tatcattttg aattatgact acaggtctga ctcccccttg
      301 ttcagatccc tgtactggtg ataaatctgc taaataaaca tctcctcgtc taatcattca
      361 ttcgttgaat tagaagataa atatgtttcg ttgcaatcgc atgcttcaca ctctatcgga
      421 aaagcttcgt tcgctaggga gagatttaaa tcagccattt gtgaatatcc ttcttttaaa
      481 gattgttcta agctatgact tctattttga ctaaaagata acatatgaga acctccaatt
      541 gattccaaaa gttattaaat gacctttaat tactctaatg ataacaaatt ttatatgcac
      601 tgaccatatt attttaacaa ttcatttgtt acaaaacgtt gatcaccatc atgatagatt
      661 cgcggcaaac gtctcgacaa gttacaaagc acttcataat taatagtatg ttgcttttca
      721 gctgccacct ctaccgactg tggactttct ctatgattat ctattaaaat caccgaatct
      781 ccagctttaa cttgatctgg cacttttaca attgtctgat ccatacatac gcgaccaaca
      841 acttcgcatt gatgaccatt tacatttaca aagctacctt gcataatgcg taaatagcca
      901 tctgcgtatc caataggtaa caatgctatt gttgttggat cagtagctgt ataagttgca
      961 ccataactta cagactcacc cgcttgtagc gtctttgttt gaactacatt agcaattaat
     1021 tgcacacttg gtttaaggtg tactttaact ttttgctgta catactctga gggataatat
     1081 ccataaaggg aaattcctgg tcttattgca ttacagaatt ggcaatccat taatagagaa
     1141 cctgctgagt tctgacaatg tatatattca ggtttaattg cttcattgac catatcttta
     1201 aaacgttgat attgttcagt tgtcatatct cctggttcgt cagcgcaggc aaagtgtgta
     1261 aacacgcctt caaatacaag ttgctcatat tgttgaatga tttcaatcac ttcttgatac
     1321 gttttagtgt ctttaatacc taaacgtccc attcctgtat ctaatttaat atgcaaccat
     1381 aactttttct cttgctcacc agaaatgttt ttaattgctt ctttcaacca ctgtttagac
     1441 gggaccgtta aggcaacacg gtgttgtatc gctttatcaa tatctttagc tggtaacaca
     1501 cctaagacta aaattttagc agtaatccca tgcattctaa gttctatcgc ttcatctaac
     1561 gttgctacag caaaaaatgt ggcaccattt tccattaaat gacgtgctac tttaacacta
     1621 cctagtccat aggcattggc tttaacgaca gccatcactg ttttatttgg atgcaatgta
     1681 ctgaatactt tgaaatttga tgcaacagcg tttaaatcta cattcatata cgcagatcta
     1741 taatatttat ctgacatatt acttcctcct gtaattccca cacgttttaa aactagattt
     1801 taattatcat tgtataacaa atttaaaatg ctgacttttc taaaacaatt tggctcatcg
     1861 cataatgctc agtgtgtgag atactaacat gtacgatgaa cccttcgtaa tcaatctttg
     1921 gtttgccaag ttcgtcgttg taacaatcta tgtcattaaa tgcgacatgt tttcctaagc
     1981 ctgtgcctaa tgctttactg aacgcttctt ttgtagcaaa cctgccagct aaaaattcaa
     2041 ttttacgttg ctcatgtgtg aaattgttga atttgtgctg ttcattttta gttaaaatcc
     2101 gctcaaccaa tttgggttgc ttactatata acacttttat tcgatcgatt tcgattaaat
     2161 ctacaccaat tccatgtatc attgattatt cctcattttt attgttttct aaatgtctct
     2221 cgttatttaa ccatcgtttc aatctacgtg cttcacttct atctattaat tcaatttcag
     2281 cttgaccacc cgctgtataa agtgtcaaag ttgctaatcg atagcgtctc attataggac
     2341 caacatcgat atcaatattt tgaatacgaa aatatggtat taccttttca tccaaaaata
     2401 aaatgccgtt tcgtacacgc aaatggtgtt tttcaaacgc atatctgcag tgcttatatc
     2461 gatagactgg cgctatgaca agcgtgaaaa caacaacaag taatattata atcacactac
     2521 taacaatgga tatatagtta tctaatatct cccaaaatag ccagttcaaa atattaaatg
     2581 ctattaaaag tacaagcgct attggtatcc aaaacagcac agcacttaac ctcatcactt
     2641 ttttagcgtg tggtgacata aaattataat ccctcatcat tttcacctct caaataccat
     2701 gatttcaatt tatttgcatc ttcacttctt gcgtatttta agttaatcga ttgggctccc
     2761 acacctttag caacaataaa actaaaatta tttaaattgt tgcgtttaag taatgtatgt
     2821 tgccaagtgt caaatcctat aatgtgatgc gctttaaaat aataaatatt tcgtttcaat
     2881 agctcgaaat tctggatagt aatttgttct tctgtcattt taaaacccgc atgtttgaca
     2941 taaagatatc ctttgatcac aaataaacca ataatgacta ctgttataat cgtaaataac
     3001 aataataatt gattccaaaa ataacagcca attcctgcca tagctatgac aataatacta
     3061 ggtattaaaa agtgtctgtg aaaacctgaa aaaggcatac cttcattaac ttgttgataa
     3121 gataaatcag gtactaaatt ctggataatt tgataggctt tgtctcgttt aataaacggc
     3181 aatatcgaca cactacctga atcattgtca tcatcttcga tttttttatt ttcaaaatca
     3241 cttgtgatga taaaatgtat tgctgtgtag ccaaataatc ttcgtatata tgactgacgt
     3301 tcaacaacag cttgcaatcg tttcgttggt acagtcactt tcttcttgtt aaatagacca
     3361 tattcaattt taagttgctc attttcaagc atcaccgaaa agccataaaa tcttatcatc
     3421 gttataatcg ttccaataat atatgccagt attaatacta gtaaaatgat gattaatact
     3481 gaaacactta caatttgaac ccattgacta atttcatgat ttagcttcga ccatgggatc
     3541 aactctctta cagcgccgta aatcggtact aaagctgcta acgttacccc aatggcgcca
     3601 ctggtcattg ccataaatag cgattcttta aaattcattt gatatatagg aattcgttta
     3661 tttttctgat taagcttact atcagagttc tgcacttcat ctagccgacc ttctgcgatg
     3721 tcttccacat taccttcaat gtcttgatta cggttgccat tctgctcagt actagacttt
     3781 tgcgccattt ctgtcttcaa ctctgtttgc aattgatcaa tatagcgttc aagatattca
     3841 ccttgttttt tcgaaataac acttaacaca ataccatcac ttggcgtttt gatttgtaaa
     3901 tccacaccac caatgatttg attgacgacc ccttgtgtca tatcaacgga ctgaatacgt
     3961 tttatattta attctttacg ctttttatta aaaatacctg ttgttaaaat aaaataatta
     4021 tcctcaatcc aatatcgcgt gttcataatt ccgacaattt gagaaatgta tgataataaa
     4081 aagaatacaa atacaatacc tatccataaa tatgattcgg gattcgtata atcaaaatct
     4141 ttcaattgaa agataacaaa aataaaaaag acgactatgt tttgtttgat agcattaatt
     4201 atgccattaa aatatgaaat cggatgtaat ttttgaggtt cagacatcac tttcaacccc
     4261 tctcaaattc gacatagttc tcgcttcgat tattttaaca tcgtcatgag acatcatcgg
     4321 taaataaata gtatgacctg cagtcataaa tccaacttta tacaaattaa gcgctttact
     4381 aattggatta gatttaatcg acaagtattg taaacgttca attcgactcg tttcttcttt
     4441 atatattaaa aatgatgtac gatattgtac acttagttga tcaatttgat aaaagcgaca
     4501 gtgatattgc cataaaggct taataaataa ttttaatgta ctcagagcac ctaaaaccaa
     4561 caaaatataa attaagtaat ttggccattc aaatcttaac catataaaat aaaaaatgac
     4621 atacacagct acactcaata taaattctat gccattcgta atgtagtaat acaacaatgc
     4681 tgacttagga ctcttagaca acttagtata aactgacata tacccctctc cccaaataaa
     4741 aaattatacg gatttataat ctatttcatt ttatttatat atgttgataa ttatagcata
     4801 tggaatattt catgctaatt tattcttcct aaaggtacat ctaaaaattt agttaagcag
     4861 aaagtgcttg aattgctaaa aaggcaccat gttataattt tatcaacatg atgcctttca
     4921 tctatattca atctttcatc ttatcaagag caatatttag ttcaagcaca ttcacataat
     4981 catttgttaa cacaccacgc tgcttacgat gttgaatcaa gtcggccact cttgaagtag
     5041 atatatgacg agcatcagca atgcgaggtg cttgcttcaa tgcattttca accgtaatat
     5101 gcggatctaa gcccgaccca gaacttgttg cagcatctat tgttacattt gaattaccaa
     5161 atttaacatg atgtttcacg cgtgctatta attcagtgtt tccattcgat tcattacttc
     5221 cacctgatga tacgccgttt ttatataatt tttcaggatt catattataa tcaactgcac
     5281 tcggtctccc gtgaaaatat cgtgtctctg tccagtgctg tccaatcaat tttgatccaa
     5341 ctatacgatt gtcatacgta attaaactac catttgcttg ttgataaaaa aatatttgac
     5401 caattaacgt gatagctagc gggaataaaa atccacataa taccatagtt attattgtta
     5461 aacaaatact atttcttatc gtattcatgg tacaggctcc ttcctcttta cacaaaaaat
     5521 tgtacaatca tatctattaa tttaatgcct aaaaacgggg cgattaatcc acctaatcca
     5581 taaatcaaca tattatttat aaaaattcta tcaatgctgt aaccctttac ttttacacct
     5641 ttcatggcaa ttggaattaa ggcaacaatg attaatgcat tgaatatcaa agctgatatg
     5701 attgcagatt taggtgacga taaatgcata atatttagtg aagtcatttc tggaatcgtt
     5761 gacatcatta gtgcaggtaa aattgcaaag tattttgcta cgtcattagc caaactaaat
     5821 gtcgttaatg cacctctcgt cattaataat tgtttgccta tttttacaac ctctattaac
     5881 tttgtaggat tcgaatctaa atcaattaga ttggccgcct ctttagcact aattgtccct
     5941 gagttcatag ctaatcctat atccgcttgt gctagcgcag gtgcatcatt tgtaccatct
     6001 cctgtcatcg caacaatatg gcctttcgct tgttcatctt tgatgacttt aattttatct
     6061 tcgggtttac actctgcaac aaatctatca acacccgctt cttttgcaat tgtagctgct
     6121 gttaaagcat tatcgcccgt acacataact gtttcaatac ccatttttct caattcagca
     6181 aatcgttcta caagaccatc tttaatcaca tcttttaaat aaatcacgcc aagcatgaca
     6241 ttgttttcaa tgactattaa tggtgtgcca cctttactcg atacatccat acagagtgac
     6301 tcaatattaa gaggaatatt gccttgttgt tgtttgacaa gatttatcat actattaggt
     6361 gcacctttga ataccgatat ttcatttgta atgattccac tcattctagt ttcagctgta
     6421 aaaggcttat atgtgccatc aatgtcttta ggcagctcat ttatatacat ctgcttcgct
     6481 aatcgcacaa tactttttcc ttctggcgta tcatcgtaaa ttgatgacat ataagcagcg
     6541 actatcaatt tttcccacat ttgttgattc actggtaaaa attcactagc gatccgattg
     6601 ccataagtga ttgtgcctgt cttgtctaaa atcattacat cgacatctcc acatacttct
     6661 acagcacgcc cacttttcgc taatacattg aattgagtaa cacgatccat gcctgcaata
     6721 ccaatcgccg ataacaaacc accgattgtc gttggtatta aacatactgt taacgcaatg
     6781 agcatcgcaa taggtaaaat taaatgcagg taagatgcta ttggatataa tgttacaata
     6841 acgactaaaa atataattgt taacgttgtt aataatgtaa aaagtgcaat ttcatttggt
     6901 gttttatttc tttccgcccc ttcaactaag gcaatcattt tatctaaaaa agatgtaccc
     6961 gcttcactct caacacgtat ttctaaccaa tcagatgtta caagtgtacc gccaatgact
     7021 ccatcaaaat cgccacctga ttcttttatc acaggtgcag attcaccagt aattgcagat
     7081 tcatcaacgg ttgctaatcc atttattaca acgccatcag caggtattgt ttctccattt
     7141 tctactcgaa tattttgtcc ggcttttaac tctgtggcgt tcactatccg atacgcacca
     7201 ttttcttcta tcaatcgagc agttaaattt gattgtgctt gtcttaaact atcagcttgc
     7261 gcttttccac gaccttcagc aaaggcttct gaaaaattag caaacaatat agttattaat
     7321 aatatgataa aaattgtaat caaataacct cgcgatagat agctagttcc aaatatgtca
     7381 ggaaaacata ttaatatcaa cgttaaaatc attccaacct cgacgacaaa cattatcgga
     7441 ttttttatta attgtttagg attcagcttg taaaaactca ttttcaaagc ttctatcacc
     7501 attgtttgat tgtaatattt atttacatga tgcattattt ccatcccttt actttatttt
     7561 aaagttaaaa attcaccaat aggaccaagt aatagtactg gaataaatgt caaaccactt
     7621 agtaaaacga taaatacgat tagtgatacg ccaaaataag gtttatcaat cgctattgta
     7681 tatttatctt gatggtatga ttttttattc actaaacttg atgcaatcat taattgcaaa
     7741 ataattggta tataacgaga aagcaacata atgattcctg tagagatgtt ccagaatgtt
     7801 gtatcatctt tcagtccttc aaaccctgat ccattgttcg ctgcagctga cgtcatttca
     7861 tacataactt gtgagatacc atgaaaagac ggattcgtta tactttcact tgctccagga
     7921 atcataaaag caagtgctga aaatactaaa attaaaattg gatgtatgag aaagactaag
     7981 acaatacatt tcatttcacg ggcgccaatt ggcatattta aatattctgg tgttttacca
     8041 accatcaaac tgcatataaa caccgtcagt aagacatata tcaataaatt catgagtcct
     8101 acgccttcgc caccaaatac aacatttagc atcattaata ccattggtcc taatccacct
     8161 aaaggcgtta agctatcatg catgttatta acagaacccg ttgtaaatgc cgtcgtaata
     8221 actgtaaata gtgctgacaa gcctgctcca aaccgtacct ctttaccttc catattcggt
     8281 ccataaatgc ctaaattcgc tagtattgga ttaccacgat actcagtcca catagttaat
     8341 gtaagaattg ctataaaaat gaaaaacatt gcgacaaata atattagcgc atgacgatgc
     8401 actcgtttac catgtctact taacatgcga ccaaataaga acaacattga cataggaagt
     8461 aacatcatac tgcccatttc tataaaattg ctccaaatat ttggattttc aaaaggtgtt
     8521 gcagaatttc ctgctaaaaa tcctccacca ttcgtaccaa gatgttttat tgattcgagt
     8581 gatgcaatag gtccaaatgc aatatgttga atatgtccgc ttaaagtccg aatcattaaa
     8641 ttagcattca acgtttgtgg tacaccttga gtcatcaata aaatactaat taaacatgat
     8701 aatggtaaaa gcactcggac aataaaccga acaatatctt gataaaaatt accaatgata
     8761 ttagtcaatc cagttaaacg tctcaacatc gctatacaaa cggcgtaacc tgatgcacta
     8821 gatgtaaaca ttaaatatgt cattacaatc atttgcgtta aatatgtcac acctgattca
     8881 ccgttatagt gttgtaaatt actatttgtt aaaaaagata ttgctgtatt aaacgctaaa
     8941 tctatcgatt ggtttaaatt atgatttgga tttaaaaaaa gccattgctg aactattagc
     9001 aatacaaatg ttataaaccc cataaatcca ttaaatgcca gaaaatgttt gacatatgtt
     9061 ttagctgaca tgtgttctaa atctgtgccg ataattttaa aacacatatt ttcaaatcta
     9121 gtaaatatta aatctactct tgacgactgc accaatgcta agcgatatag atatccacta
     9181 aaaacatacg caatcataac catcattgtt agaaacaaaa ttatttccat gataaccctc
     9241 acttaataaa tttctaaaat ttttcactac caattaaggc ataaaataaa tacaaaacta
     9301 atgcaataac taccaataat aaaacgatga gcattgccat aacctcctta caacacaaca
     9361 acatcgtaac aacttgttta tgagagaaac attaattttc aaacttagtt attaagaaat
     9421 cattaagatg tgtatgcaga aataaatttt atagcattta attgtgaaga atattatgat
     9481 attgctatcg aggtgaaggt tatgtcaaac actgaatcgc taaacatagg aaaaaagcgt
     9541 ggatctttaa caatttacat cggttacagt ccaggtgttg gtaaaacatt tgagatgctt
     9601 tcaaatgcca ttgaactatt tcaaagtaac gttgatatta aactaggata tattgaaccg
     9661 catcaacgtg atgaaacaaa tgcattagct gaacaattac ctaaaatcgc aaccaatttt
     9721 actaaacatg gtagtcatca ttttcaatat ttagatgtcg accgcataat cgaagaatcg
     9781 ccgacaatag tacttatcga tgagttagca catacgaaca tttctagaga tcgtcatgaa
     9841 aaacgatata tggatattga agaaatttta aatcatggta tcgatgttca tactactttg
     9901 aacattcaac atattgaaag tttaagtagt caaattgaac tgatgaccgg tgtatatgtt
     9961 aaagaacgtg tacccgacta tttcataatg agcgccgatg tattagaagt cgtagacatc
    10021 tcacctgaac aattaattaa acgcttaaaa gctggcaagg tatataaaaa ggataggcta
    10081 gatgtagcat ttagtaattt ctttacgtat gcccacctaa gcgaactgcg tacattaacg
    10141 ttaagaacag ttgccgactt gatgagtgat aaagaaaaag tccgacacaa ccataaaacg
    10201 tcactcaaac ctcatattgc tgtggcaatt agtgggagca tttataatga agcagtaatt
    10261 aaagaggcat tccacattgc tcaaaaagaa catgcaaaat tcactgctat ttatatagat
    10321 gtattcgaaa aaaacaggca atataaagat agtcaaaagc aagtgcatca acatctcatg
    10381 cttgcaaaat cattaggagc aaaagtaaaa gtagtttata gccaaaccgt tgcattagga
    10441 ttagacgaat ggtgtaaaaa tcaagatgta accaaattaa ttatcggaca acatattaga
    10501 aataagtggc gagacttttt caataaacct ttaattgacc atttaatgtc ctttgaacat
    10561 agctataaaa tcgaaatcgt tccaatcaaa caaatacctg ttgaattgaa aatgaacaag
    10621 tcaccctatc gtcctaaagg caaacgtttc gccatagata tgttaaaaat gattttgatt
    10681 caaataattt gtgtaatgat gggactatgg atttatcaac ttgataagca tgagtctagt
    10741 acgattattt taatgatttt tctcatcggc atcattttat tatccatttg gacgcggtcc
    10801 tacatcatcg gctttttagc agcaattatt aacgtatttg tttttaatta tttttttacg
    10861 gaacctagat atacatttga agtataccgc tttgactatc ctattacatt tatcgttagc
    10921 attttaacaa gtattttaac gagtgcttta ttaaaacaaa ttaaattcca atactccatt
    10981 actaaaaagc aactctatcg tacagattta ttatttcaat ttaatgattc gattaaacaa
    11041 acttatacag ttgaaaactt actaataaat gctggatacc aaattaatca attattgcaa
    11101 caatcaatta ctatatacgt tatcaatcaa tccaaagtaa ttaaaacaat accattgcaa
    11161 aaccatatcg ataatacgac tcaacagcat gaacaagcat taagctgggt aattaaaaat
    11221 gaaagacaag ctggtgcaac aacagataca tttccaggca ttaataaatg gttgatacca
    11281 attggcacat ctccgattaa aggcattcta gccattgatt atcaaagttc tcaagtgatt
    11341 aatccatatg atgcatcaat attagaatca atgttaaatg aactttctct cgccgttgaa
    11401 aatgtgacgt tacttaaaca aacgagagaa tccatgttac aggctgaacg tcaactgaca
    11461 cattcaaatt ttttaagatc aatttcgcat gacatacgta caccgttaac aacaattatg
    11521 ggaaatttag atattttagt atcacatagt aaagatatgt ctatcataga aaaggaacaa
    11581 ttactcgtgc atagttttca agaaagtcag tatttatatc tattagtgac aaatatttta
    11641 tcattaacaa aattacagtc atcaaatgtt cagataaaat tgcaacctta tctcgttagt
    11701 gaattagtag aagaaatcga tatgatttta gaacgtcgcc atttaaaaaa gcgtataact
    11761 gtgtcatcct cagtagattt acagtttata cacattgaca gtaagttgat tttacaagct
    11821 ttattcaatt tgattgaaaa tgcagttaaa cacacttcta ctgacaccaa aatcaattta
    11881 tctattcgtt atgctagcga tgaacaaatc gaatttgccg ttatagacga aggacctgga
    11941 attagtttag aagaacaaca aaagatattc gagccttttt acacaggatc tttaaagata
    12001 accaaaaaga aagcatggga ctaggcttat atttagtaca gactatccta cacaaacatc
    12061 aatcaaactt acaatataaa ccaaaccaac cacacggcag tatattctat ttcaatatag
    12121 atacagactt taatgaagga gacatataat gcaatctaaa atattgataa ttgaagatga
    12181 tcacgcaatc acacatttac ttgatgttgc attaacttta gattattaca atgtaactac
    12241 agccgaaaat gccacacaag cacactttaa aattcaaatt gataaaccag atgtcatttt
    12301 attagattta ggtttaccag ataaagatgg attatgtttg atttcagaaa tcaggcaaca
    12361 tactgacatt cctatcattg taataagtgc aagacaagaa gaacaaacaa ttattcaagc
    12421 tttagataac ggtgcgaatg actatatgac taagcctttt aatgttgatg agcttcgggc
    12481 acgaattcga gtcatcgaaa gaatcgctaa gtcacatcaa gaaactaaca ttgtatttac
    12541 taacggtttg ctaagcatcg actttggctc caaatctgtt gttattaata atcaggaggt
    12601 acatttgaca ccaaatgaat ttagcttgtt agaactattg tcaaatcata aaggaaaagt
    12661 actcacttat gagatgatat taaaacgaat atatggctat gttaataaga ctgaaatgcc
    12721 tagtttaaga gtgcatatga catcattaag acaaaaactt tcacaatgtc atgaggacgc
    12781 aaaagatatt attaaaacac atccacgcat tggttatcaa atgttgcagt ggaaagagaa
    12841 gtaattaaat aaaaaagatc gctgccatta aacgaatgat tggcagtgat cttttttata
    12901 ttcaacttat tatttatcgc gcactagtgt cagcaaaagc ccctattgtt ttttacaaga
    12961 acatgatact gaaatttcat aaagtttatt tatgttcgat tattcttaat ttaagtagtt
    13021 gttatattct agattcatat ttatatttac acagaatgat ataaccgcac acagatctca
    13081 atccaattaa atatttaacc tttaaaacgt tagtactttg atatctacag agaatcagtt
    13141 attgaaaaca aaaaagagct caagacatta catctttaag ctcttaatct ataaattatt
    13201 tttgatggtc agcaaatgtg cgacctttca taggtctgct tccaccgctg ctcttctctt
    13261 tacggtcgaa ttttttgttg ccttttttct tgctagagta tccttttgaa cgtttactct
    13321 taccgtcaaa tttaggatta ccacgcttac tatttctgtt acgagaacca cttggtttac
    13381 cgttacggcc tttgcgagat aatggttttt caaaagttaa ttgaacttca acttcatcgt
    13441 tcgcttctac taactcttgt aaaagtgcag caactaaatc aacatcgtta tattcattta
    13501 acaactctgt agatatgcgt ttcaagcgtg attctgactc tttagacatc cagttttcaa
    13561 ctttttcttt gatgtcatct tcacgtgctt gaagtacttc tttacgatgt ggtggacgaa
    13621 gtgcgctcat ttttctacca ttagcatctt caatttgtct aatgtaatcc atttcaattg
    13681 gatttacaaa tgttaccgcg ataccttctt taccagcacg acccgtacga ccaatacggt
    13741 gtgtatagct ttcagtatct tgaggtatat caaagttata aacatgactc acaccagaaa
    13801 tatctagtcc tcttgctgct acatcagtag cgactaaaat attaatttgg tcatttttaa
    13861 atttctttaa tacttctaaa cgtttcgctt gtgtaatatc accatgtaag ccttcagctt
    13921 tatatccttt agaaatcaaa gcacttgtta attcatcaac acgacgtttt gtacgtccga
    13981 atacgattgc taattcaggt tgatgaacat ctaggaaatt tgtaaatgta tcaaatttct
    14041 ctaattcttt aacaattgta tagaattctt cgatttgagg atcagacatt tcattattca
    14101 ttgtcttaat gatttttggt gatttcataa attgttgtac taaagcttgg attgctttag
    14161 gcattgtagc tgagaacaac attgtttgac gttgtactgc tggaatttta tccataataa
    14221 atctcatatc atcgatgaat cccatattca tcatttcatc agcttcatct aaaatcaaag
    14281 tatgaattcc gtccgttttt aatgtgcgac gatttaaatg gtcgataaca cgcccaggtg
    14341 ttccgactac gatttgtggg ccttttttca aggctttaat ttggcgttcg ataggcatac
    14401 caccgaatac agtaacaact tggacacctt gtccacggct aaattctctt aattgttcag
    14461 ctacctgcat tgccaattct cttgtaggtg ctaaaatcaa cgattgaacc ccttgtttcc
    14521 ctactacttt ctcaattaaa ggaataccga atgctcctgt tttacctgta ccggtttgag
    14581 cttgtccaag gatatcaatt ccttgtaacg cataagggat actgtctttt tggataggtg
    14641 tcggctcttt aaatcccatt gattcaagtg actgaaccgt attatccgaa atccctagtt
    14701 ctttaaaatt ttgcaaaata attctccttt ttacctgttt atttctcaat atacttttgc
    14761 atataagttc attaatattt catcaacttc tatatagtac caccaaaaaa atttaatcgc
    14821 aataaagcaa tttgcgaata tttattagaa tcacattaat tgattgtcat atttttgtca
    14881 aaaagctagc attagataag atgtaagcgt tacgcatcat tcgaataatc attataaaaa
    14941 attttttata aaatacgtat atcgttaaca ttccacaaca aattttactg aaacacatgt
    15001 gaaattagac attataatac tacatctttt tgtaaaatat tatataaaca ttcaaactac
    15061 ttcctatata aagtgtgtac atagaattaa ccttgattta gtttcaaatt tatagattta
    15121 acataaaaag aaaaaggtaa ttttgtattt acacacaaaa ccacctcttc tttgtaatct
    15181 tttagtatta attcaaatca tttttataag taaaaggtcg ctcgactaat ctctatgaaa
    15241 ttaaagcatt tacgacttct tctaacttca taccacgtga tcctttaact aatacacaat
    15301 catgcgcttt taaatcattt gttaaaactt ctatcatatc gtctttagaa ttgaagtgtt
    15361 gtgctttttc gacatgttgc tggcccgaat cataaatata cttcgcttca ttaccaaacg
    15421 tatacaacac atctatatgc ttttcttcta aataattacc tacaccgata tgcatttctt
    15481 tgctattttc acctaattct aaaacatctc ctaaaattag aatgcgacgc cctgtcaaag
    15541 tactcagtgt atcaatagct gctctcatac ttgtaggact tgcattatag gcatcattta
    15601 tcacagtaat atcattttct aatgtgtgtt gctccatacg cataccagtt aagctgacat
    15661 tttttaaatt ttgatagatt gtgttatatg tcaaacctaa ttcatgacca actgcaatgg
    15721 caatcgtcgc atttttcata ttatgctttc ctaatattgg cagatcataa tgttctttat
    15781 tattaatcgt aaatgaaata cctgtagtat ctctatcatc aacagaacaa actaatgtat
    15841 tatcagtagc aacaccaata ctaatacatt ttgcattttc aacttcttta acatgcggtt
    15901 tcaataatgg ttcatcgcca tcatatataa acgtaccatt atcttttaaa cctattgtaa
    15961 tttcagattt agctttagca atcccctcgc gcgaacctaa atcttgcata tgtgactcac
    16021 caatattagt tataactgca atatctggtt gagcgaggtt tgacagaaat tcaatttcat
    16081 ggaaacctga catccccatc tccaatattg atatttcagt atcattatct aattccaaaa
    16141 tagttaaagg taaaccaatt tcattattgt aattaccttg cgttttctta actttaaatt
    16201 cggtatgcaa tacactttca atcatatctt tagtcgttgt tttaccatta gaccctgtga
    16261 cggcaattac tttagggttt acatgtctca agtaagcttg tgccaattgt tgtaatgctg
    16321 ttaatgtgtc ttcaacccat ataacaggac cgcttacatt ttcatctata ggtgtccctc
    16381 tttgataaaa agcagctcca gcaccatctt gtaatgcttt agagacaaag cgatgaccgt
    16441 caacattttc acctttaaat ggtataaata acatattttt agaaattgct cgtgaatcaa
    16501 ttgtgactcc atttatctct tgatttaaaa attgatcttc aatttcacaa ggaatccatg
    16561 attgaatttg ctttaatgta acattaatca taataacaac ctcagttagt caattttgta
    16621 tttatttttc tgtttatcct ggtgacgttc tttagcaagc tcgataagtt ttgtaatcaa
    16681 ttctggataa gataagccca tattttccca taattttgga tacatactga aagccgtaaa
    16741 tccaggcatt gcatttgttt cattaatata tatttggttg tcttctgtta caaagaaatc
    16801 agcacggact aaaccagaac aatctgtcgc tttgaatgcc tctaatgcca tatttctaag
    16861 cgttaattga acatcttcgt ctaagtcagc tggaatttgt aattgaacct taccatcttt
    16921 atattttgat ttgtaatcgt aaaacgcgac atcttttacg acttcacctg gccatgtcgc
    16981 ttcaggatag tcatttccta aaactgctac ttcaatttca cgtgcgttaa cgccttgttc
    17041 aataacaagc ttacggtcaa attggaatgc ttctttaata ccttctttaa gttccgcttc
    17101 attattacat ttactgatac ctacacttga ccctaagtta gcaggtttaa caaagactgg
    17161 gtaatttaat ttatcattta ctaattttaa aatgttatgt tcatattttt catattcaga
    17221 acgtaagaaa ctaatataag gtaactgtgg taaccctcga tgttcaaata attgtttcat
    17281 tacaagcttg tccatagaac ttgcagctga caatacacca tttcctacat atggtacatc
    17341 caaaacttca aaaagtcctt gaatcgtgcc atcttcacca ttaggaccat gtaataatgg
    17401 gaatactgca tcatatggtt gtcctgaact actttctttc aatagctgtg aaatctctag
    17461 cgcctctcca ttttctaaat gaagctcatc agtagattta atttcagctg taatattatt
    17521 ttgctttctc caatcaccat cattggtaat ataaatgata tcaacatgat atttgtcttt
    17581 atctattgca tttaatacat tttgtgctgt cagaatcgat acttcgtgtt ctgcactttt
    17641 ccctccaaaa acgatacaaa tattttcttt tgtcatttcg ttttcctcca attatatatc
    17701 agggtttgct accttaatat aaattaatat tgttttagtc ctattgttaa ttgatttatt
    17761 cgcatctttg aatcttaata gtatcacatt ttaatgctat tttgcatttt aaaaacgagg
    17821 cgttatgtgt ctgtatctat gttgattcat ttataatcag aacaatattc ccttctattt
    17881 tattcctaat tcaaattgaa atctatagct gatatcactg tgatattcta gctaattttt
    17941 aaaaagtcat gtaaaattga tataaacatt agtgagtata aaaggagttt gcaatgaatt
    18001 attcatctcg tcaacagccg gataagcatt ggcttcgcaa agtagactgg gtattagtag
    18061 ccactatagc tgttttagca attttcagtg ttctgcttat taactcggca atgggcggtg
    18121 gtcaatacag tgctaatttc ggtatcagac aaatttttta ttacatttta ggtgcaattt
    18181 ttgcaggtat catcatgttt atttcaccta aaaagattaa acattataca tatttattgt
    18241 atttcttaat ctgtctttta ttaataggct tgctcgttat tcctgagtca cctattacac
    18301 ctattatcaa tggtgccaaa agttggtaca cgtttggccc tatcagtatt cagccatctg
    18361 aattcatgaa aattatttta attttagcat tagcgcgtgt cgtttctaga cataatcaat
    18421 tcacattcaa taaatcattc caaagtgatt tgttattatt tttcaaaatt attggtgtct
    18481 cgttagtacc aagtatttta atattactgc aaaatgacct aggaactaca ttagtattag
    18541 ctgcaattat tgcaggtgtg atgttagtaa gtggtataac atggcgtatc ttagcaccta
    18601 tctttattac aggtattgtt ggtgcaatga cagtcatttt aggtattcta tatgcacccg
    18661 cattaattga aaatttatta ggtgtccaac tgtatcaaat gggacgaatc aattcatggc
    18721 ttgaccccta tacatatagt agtggtgatg gctatcattt aactgaatca cttaaagcta
    18781 tcggttctgg acagttacta ggtaaaggat acaatcacgg tgaagtttat atacctgaaa
    18841 atcatactga ctttatcttt tcagtgattg gagaggaact aggttttatc ggttctgtca
    18901 tattgatctt aatattttta tttttaatct tccatctaat aagattagct gcgaaaattg
    18961 aagatcaatt taacaaaatc tttatcgttg gtttcgtcac tttacttgtg ttccatattt
    19021 tacaaaatat tggtatgaca attcagttgt taccaatcac tggtattcca ttaccattta
    19081 ttagttatgg tggtagtgcg ctatggagta tgatgactgg aataggtata gtcttatcaa
    19141 tttattatca tgaaccaaaa cgatatgtag atttatacca tccaaaaagt aattaattta
    19201 aactattttg agtttcaaat attataactt ttcaagatga cgttatatag tctatttatg
    19261 tcgtcgattt aaaatgtcat atatagatat tactcgataa taacaatccc tctttgaagt
    19321 acacattgta aaatgtacac tctaaagagg gattttgtta tatagtactt gctttcattt
    19381 tagtaaatac agtcaaaaaa gctatcatcg aatagtccga cgatagcttt aacggtgtgt
    19441 gattcacaat cacagtcaat tattttaatt cgtgacttac tttattagat gctggcaaag
    19501 tattaataat gtctaaacga gatttagttt ttttaatatt cacacctaat gatgacaata
    19561 gtttgactac attttgaatc ttttcaggac gtttcattat tatcacctcg tttggatcgc
    19621 tttcaatatt atcttaccct attcgacata cctttactac atcatttaca aattttttaa
    19681 tttattttca aaattaaaat ctatagcgca ctctatatta taccctaaaa tacaattttg
    19741 tatccttcat catagatttc tttttcaata ctattcaaat ttgctggtgt ctcatacgat
    19801 acagttactt gaccatctat tatattaata ttcacttgat gaacacctat catatttgaa
    19861 atgcgttctg tcaattgact tacttgttct tctgtttcaa ttcccgctgt gtaaatcgta
    19921 ttttgatgta tcatcttaaa ctcctttagt ctttaatcaa tttttgaaaa gtcactaata
    19981 actcttccat tgcctcttcc tgagcacctt gattaacttt attcataata caacttttca
    20041 tatgttgttc taataacttt gtcgcaacac tgtttaacgc cgaacgtgtc gctcttattt
    20101 gcgtaaggac atcatcacaa tagacatctt cttcaatcat gcgattaatc gctctcactt
    20161 gtccttcaat tcgatttaaa cgtgatttaa gattcgtttt tatttgttca gaatgatgtg
    20221 cattatcttg ttcagtcata gcattcactc cttcatttac attccttact atactatatc
    20281 ccataaaaca caacgtcaac acaaagtgtg tttttcaaac taaattgtca tgatatttaa
    20341 attgcttcat gatacaatta ataagatata ctactcgttt aagattttcg atgggaggtt
    20401 tcaaatgata gagttattat ccattgcact caagcattct aatattattt taaattcaat
    20461 atttattggt gcatttattt taaacttatt attcgccttt accattattt tcatggaaag
    20521 acgttctgcc aattctatct gggcttggtt actagtctta gttttcttgc ctttattcgg
    20581 cttcatttta tacttactat taggacgaca aattcaacgt gaccaaattt tcaaaattga
    20641 taaggaagat aaaaaaggat tagagttaat cgttgatgag caattagctg ctttaaaaaa
    20701 tgaaaacttt tcaaattcca attatcaaat tgtaaaattt aaagaaatga ttcaaatgtt
    20761 gttatataat aacgcagcat ttttaacaac agacaacgat ttaaaaatat acacagacgg
    20821 ccaagaaaaa tttgatgact taatacaaga catccgtaat gctactgatt atattcattt
    20881 tcagtactat attattcaaa atgatgaatt aggtcgtacc attttaaatg aacttggtaa
    20941 aaaagcggaa caaggtgtag aagttaaaat tctttatgat gacatgggtt ctcgtggact
    21001 gcgtaaaaaa ggtttacgcc cgtttcgcga taaaggtgga catgctgaag catttttccc
    21061 atcaaaatta cctttaatta acttgcgtat gaacaatcga aaccatcgaa aaattgttgt
    21121 aatagatggg caaattggat atgttggtgg ttttaatgtt ggtgatgagt acttaggtaa
    21181 atcaaaaaaa ttcggctatt ggcgagatac gcatttacga attgtcgggg atgcagtaaa
    21241 tgcattgcaa ttacgattta ttctagattg gaattcacaa gccacacgtg accacatctc
    21301 ctatgatgat cgttatttcc cagatgtaaa ttctggtgga acaattggcg ttcaaatagc
    21361 ttctagtggt cctgacgaag aatgggaaca gattaaatac ggttatttga aaatgatttc
    21421 atctgctaaa aaatcgattt atattcaatc tccctatttc atacctgatc aagccttttt
    21481 agattctatt aaaattgcgg cattaggtgg tgttgatgtc aatatcatga ttcctaataa
    21541 acctgaccat ccgtttgttt tttgggctac tttaaaaaat gcagcatcct tattagatgc
    21601 tggtgttaaa gtatttcact acgacaatgg ctttttacac tcaaaaacac ttgttataga
    21661 tgatgaaatt gcaagtgtgg gaacagctaa tatggaccat cgcagtttca cattgaattt
    21721 cgaagtcaac gcctttattt atgaccaaca aattgccaaa aaattaaaac aagcttttat
    21781 agatgattta gcagtatctt ctgaattaac aaaagcacgt tatgctaagc gaagtctttg
    21841 gattaaattt aaagaaggta tttcacaatt attgtcacct atcttataaa atagaaatat
    21901 gaggagtgta actttaatgc aacaatcaga catcattagt gcagccaaaa aatatatgaa
    21961 atctattcat cataatgatt atacaggcca tgatattgcg catgtatatc gtgtcactgc
    22021 tttagctaaa tcaatcgctg aaaatgaagg tgttaatgat actttagtca ttgaactcgc
    22081 atgtttgctt catgataccg ttgacgaaaa agttgtagat gctaacaaac aatatgttga
    22141 attgaagtca tttttatctt ctttatcact atcaatcgaa gatcaagagc acattttatt
    22201 tattattaat aatatgagct atcgcaatgg taaaaacgat catgtcactt taactttaga
    22261 aggtcaaatt gtcagggatg cagatcgtct tgatgctata ggcgctatag gtgttgcacg
    22321 aacatttcaa tttgcaggac actttggtga acctatgtgg acagaacaga tgtcactaga
    22381 taagattaat gatgatttag ttgaacagct gccaccatct gcaattaaac atttctttga
    22441 aaaattactt aagttagaat ctttaatgca tacagatacg gcgaagatga ttgctaaaga
    22501 acgtcacgac tttatgatga tgtacttgaa acagtttttt acggaatgga attatcacga
    22561 ctagacattg gagttgtagt atgatgatgc gatgtaatgg cgtattgttg tggaagcttg
    22621 gtgtcatgcc atgttacttt gatgtgctgg tgctatgatg cggcgttgcg gtgttatggt
    22681 gttatagacc ggcttggtgt tgatgccatg ctactttgat gtgctgttac cgcgatgcga
    22741 cttgatgtag tgctatgatg cgacgttgcg gtgttatggt gttatagacc ggtttagtgt
    22801 tgatgccatg ttactttgat gtgctggtgc tacgatgcgg cttgatgtag tgctatgatg
    22861 cggcgttgcg gtgttatctt gttatagatc ggtttggtgt tgatgccatg ccgttacgat
    22921 tctatgatat gttgttggga cgttgcaatg tgtattatgc cgttgtgacg ttattatttc
    22981 acactgttac atgtattagt gaattgctgt ggaaatgttg cgaaatatac tgctacactg
    23041 atgaatcatt gtgtcaagat gacattgcaa tgaagagagg ctactctgtt attaaccact
    23101 ttttacatac tgaaatctcg ttaatattat ttcaaataaa aacagcagta ggatgacttt
    23161 cacatttgaa atcatcttac tgctgtttct atttatcaca tattgtataa tgtgacacta
    23221 agtttcgcta ttgaagcgaa aaataatgtg cgccctataa agttaaaatt atcttcaact
    23281 tttagggggc acaatatttg gacttgctaa ggttatttct ttttcttttt agacactact
    23341 tgtgtgtttt tgcctttttt attgctgccg ccgttgtgct ctctttcata cgcttcaatg
    23401 aaaggttgta cttctttttt agcgactttt tcataataaa tgttcgcaaa gtgtgtttga
    23461 actacaagga acgccgcact gactgaccag tacaaaccaa gtgctgatgc tgagcttaat
    23521 gaaatccaga taatcataat tggtgaaatg accatcatca tgtaacccat ttgacgttgt
    23581 tcgtctggca tcgttttact tgatacatat gcttggataa agtataaaac accggcaata
    23641 attgtaatcc aaatatcagg acgtcctaaa tcgaaccata agaagtgtgg atatttaaac
    23701 aaaccatcta caagttggtc tttaagtaca aagtataatc ccatgatgat tggcaattgg
    23761 attagcattg gtaaacaacc caacatactc ttaatcgggt tcatgtcata ctttttatat
    23821 acttgcatta attcttggtt tgcagccatt ttttcttctt gtgtacgcgc acgtttcact
    23881 ttttcttgaa ttttttcaac ttctggcttt gcaactttca ttttttgacg catcatatga
    23941 ctatttttat agtttgacaa catgaatggt aataaaataa tacgaattac caatacaagg
    24001 ataataatag ctaaaccata attgtcgttt aataagttat ttcccaacca atccaataca
    24061 tttttcattg gatctacgaa tgtattgtag aaaaacccac tacgtttttc aggtttagaa
    24121 tagtcacaac cagccaaaaa gaccataata cctaaaaata atggtagtaa cgcttttttc
    24181 ttcatttttc cacctctatc attatattca cataggattt attctatcac attaatgagt
    24241 acgtatgaaa caataagtgg aaaaatttaa ctaattatta aaaaaatctt tgaatcgatt
    24301 aacagtcttt tcaatatttt cacttttaga aatggctgaa atgactgaaa ttccattggc
    24361 acctgcttct acaatcggcg ccacgttact agtatttata ccgccaatag ctacaatcgg
    24421 tagttgcgga ttcatttctt taaatgttgc aatcatttct ggacctactg gtgtatgcgc
    24481 gtcatgtttc gacggcgtag gatagattgg tccaacacct atataatcaa catgagttaa
    24541 atcagatttt gcatactcat ctaaatcact aatacttagt ccaataattt tatcagtgaa
    24601 atattgtgct atctctttga ctttcgcatc atcttgaccg acatgtatac catccgcgtt
    24661 aatttctttt gctaaggata catcatcatt aacgataaaa ggcacatcat attgatgaca
    24721 tagatgctgt aattctttag ctaatacaag tttatcgttt ccttttaaag ctgattcacc
    24781 cttttctcga aattggaaaa gtgtaatccc tgcctttaat gctgcttcta atacttcatg
    24841 aatagttcga tgactcggaa catcggagat cccacaaata aaatacacat ttagatacga
    24901 ttggttaaac atattattcc acctcttgaa tgcgaatccg ttgttgatag gttgtttcat
    24961 ttaaatgata taacgtatca agcaacaatg gtgaaaacgt accaggacca ccacaatttt
    25021 cattttcagc agctacctca gcagcaatat taaatacgct taccgcttca attaacgctt
    25081 ctatgtctgg ttctgtttct ctaaataaaa atccagcaat aacgcctcct aataaacaac
    25141 cggctccagt tactcgtgct aataatggag atccattagc taatacgatg gctttatcac
    25201 cttgaacaat aacgtcctct ttacctgtga ttactattgc agttttatat attgcgtaag
    25261 ccttttttgc tattgcaacc gcatcaagat tagcatcact atctgtacct ttcatagttg
    25321 ctgtatcatc aatcaacgct aatatttcag atgcattacc tttaattact gatactttcg
    25381 ctgatttcaa taataattta caaaattgct ttcgatatgt agaagcacca acagctacag
    25441 ggtcaaatac aataggtaag cctgcctcat ttgccgtttg agcaatcgca ataatatctt
    25501 gttcattttg tgctgttaaa gtaccgatat tgattaatag cgcttgtgca actttgtaaa
    25561 attcttcagc ttcttcggga gcttcactca ttgcagggct cgcaccaata cttaataaac
    25621 cattcgctgt aaaattttta actacatcgt ttgtataaca aattgttaat gggttttcaa
    25681 tacgtatttt atttagataa ttcatcgtct aatccctctt tctttaaata tgcaaaatga
    25741 ttcactggac ctctaccacg accgatttca ggcgtatatt gtatactcat tgaaataaac
    25801 tttttggcct tgtgtacagc ctcaaataat ggtctacctt ttgcaagttc tgccgttata
    25861 actgctgaaa atgtacaccc tgttccatgc gtatgttttg ttttaaatcg tttattttca
    25921 aatgtttgaa caccttcttt agtaaataaa taatcttttg ctatatcagt atcatttgaa
    25981 tgaccgcctt taatgatgac accattacta ccaatctcat taataaagat gcggccagcc
    26041 tgcataattt tttcttcact atcaatggtt agtcccgtta tttcttcagc ttctggtaaa
    26101 ttcggtgtta ctacatcagc taatggcaat aacgtatgct gcaagttttg ctttgtgtca
    26161 ttatccatta atgaatcacc gcttttcgcc aacataacag gatcaattac atacggaata
    26221 gattcatgtt gcattaaata atgacgaatc gtttccatag tatctgctgt agcaatcatc
    26281 cccgttttaa tagcatgcgg taaggtatcg ttgaagacac tatcaagttg ttcatctacc
    26341 cattgatgat ttaaattatg aatatgttgt acgcccaatg tattttgagc aacaatactt
    26401 gtaacgacgc tcataccata tacaccacat gaatgaaatg attttaaatc agccattacg
    26461 ccggcgccac ctgttggatc agtacctgca atggttaatg ctattttagg tttaatcatt
    26521 tactttccct ccaaattccc attgttctag tgtcatagcc atattgaaaa atcgacgctc
    26581 atgtatgcaa ctttctaaaa atacttgctt cacttgttcc aattccttgt cagacattga
    26641 ttcggctaat ttattcatta atgcctcaaa tacgttaatt atgtcatcca tttcagtact
    26701 ataaaaatca aaccattttg ccgtatcttt ttctctatta agcttatgat cactttgaga
    26761 gagcttagct aattctgcat aaatatatgg acaaggtgcc attgcagcaa ttgtataaat
    26821 agcattttca cgactatgcg cttggaaata catatgtttt atgtaatggt cgccacttgg
    26881 aggccaaact tttgttttaa tgatttcttc gtatgattca ccaacaattt gcgctaaaat
    26941 atcatgcgca agtacttcac cttcaaccat aaattctatt tgctctacta aaaattttac
    27001 atcattcata ctattcattt tcggaattaa caaagcatat aagtttgtga attcttttaa
    27061 ataagctgca tcagcttgta aataatgacg taatgcatca gcttgtatat tgcctaataa
    27121 cattttttga ataaagtcat cctcataaat atcattaatg attggctttg cagcttggta
    27181 caatttttgt gaaaattcca ttgtaaaaaa tcctccctaa ataaaaaaac tacttccaac
    27241 atgaaagtag tttgatggca atgttgctat actagccaca tcacttcaat aactactttc
    27301 ctacgttggt actaaccaaa tcaggtcata agggtctgaa cagttcatct cagccatatc
    27361 gttaggctcc cctagtagtt ccttagtatt caattgcaaa ttaatcttag caaacggttt
    27421 caacattttc aattattgtt gctcagttgg gagtgggaca gaaatgatat tttcgcaaaa
    27481 tttatttcgt cgtcccaccc cggcaaggtt gactagaatt gaaaaaagct tgttacaagc
    27541 gcattttcgt tcagtcaact actgccaata taacttcgta gagcatagaa cattgattta
    27601 tgtcccagcc tgtataatta tatatatttg cgaaataaaa aagaccggaa catatcgaga
    27661 attccccgat atattccaat ctaaaagtta cttatataac tattggttag ctatgcataa
    27721 atggcttatg cagtaaccca atgtccagcg ccaccagtgt tatataattt tactgctgcg
    27781 gcatcttgaa cactttcagg agcatttgct ggtgatacac ctttgtattt agcaggtgct
    27841 actgaatccc aagttgattg taagaattga tacttaccag ctgcacctga tgttggattt
    27901 acagcatgaa tattgccacc tgattcacgt tgagcaattt gttttagatg agcattcaca
    27961 tttactgatg aaccttctga tgcttttgat tcagttggtg ttgcagtaac ttgtgaattg
    28021 tttgatgttg atgcttgtgg ttgttgagtt tgagcatttt gtggtgcttc aacttcttgt
    28081 gattgtactt gattagcttg aacagctgat ggtgcaacat tattagttgc aggtgcttgt
    28141 acactcatgt ctgctccatt agcacctgtt gcatggtaat tccaagcaaa gtgtgtacca
    28201 tctgattcaa agtgataagt aaacccttca tagtcaaatg tataattata agcgccagct
    28261 tcaattggtt tttgatttaa tgtttgatca tttgattgcg ccatttgcgc taaagatgct
    28321 ttatttaagt ccgcttcact tgcatgggct tcgtgacctg catttcctgc tacgattcct
    28381 aaacctactg ctaatgatga tgcgagtaat gttttcttca taatcttaaa atcctcctac
    28441 aagtaatttg tttctctaaa agttttacag agactactgt aacatctgta aaatccgtgc
    28501 aggttacaac aaaataaaaa aatagccttg ttttttacac agtgattaca acgaaatccc
    28561 ccataatttc aaatatgatt ctatcgagaa ttgtcatgac agtaaaaatg tttttcattt
    28621 gcaatttttt atgtcatcgt tttgcaatct atgtctattt tctattagaa aaactacaca
    28681 aaaagacgac ctacacaaca gtactagtca tgtaagtcgt ttttaagtga gtatatttca
    28741 agttaattag atctataaaa atttaggatg gattaaatgt atattaagga tgtttaggat
    28801 aattggattt aaatttctaa taagtcatga ttatctatat tttgagagtc aatatctaaa
    28861 atactatctg agagaatttc attattttgg gatttagggg acataaattt tattgtttca
    28921 acatataatt cagtgacaaa gtgtgtttgt ccgtctttat catactttct tgatctcatt
    28981 tgaccagtta taccaaccaa tgtaccttga ttagtatatt tttctatatt agaagctaac
    29041 ttgccaaatg ctttacagaa taagtaatca cagacgattt ctccattttc atctttataa
    29101 tttcggtgcg ttgcaacaca aaacgttgca atttttctat cctccttttc aaatatttgt
    29161 gcgtctttcg tcagtctccc gacaattacg attttattta gcatgtagtg tgtgattcac
    29221 ctcctatgcc tatatcttaa ttactagctt aaaaatggtc aaaatggcaa attttatata
    29281 attaatagct atagcatctc gttatttgtt atatttaaag aaattagtca tgaaatgacc
    29341 ttaaatcttc taaataattc aaaaatcata aatattaaac taatgggatg tgggaatatg
    29401 aaaactttta aagcagtaag gtttcaaatt gtgaatgaac acggcagaat tatagaatat
    29461 gaattagaag atggcgtaat aattaataaa gaggaaagtg gcacgggttg gttgcttgaa
    29521 attgtcattt ctaatgagca ttatgaaaca tttaaggaat atcaagacag tgaacagtta
    29581 ttggatattc gtgtcgtcat cacgcgtcca gccaatgatc cagctttatt tgaatctact
    29641 gtgaaaagta ttaaaaattt taaaacaaca atgtctatcg tatttgaatg tcatatttac
    29701 actttacgtc aacagtatgc tgaaagttta ttagaacaac ttattgatga tggattatct
    29761 ggtgaagaat taaaaaaatc atttaatcga atgatgcagt ctaaaccaaa gttaaaggat
    29821 gaaaaattag aagagtaaaa taaaaaacat tcaaagatta tgccaacaca ttaaatcttt
    29881 gaatgttttt tgttttattt tacatcttga attgcaaatg taagttcaca actacaagca
    29941 agttgaccat cgacagtagc tttagcatta cctttaccga ttggtccttt aattttagtg
    30001 atttctactt ccaacgttaa agtatcacca ggtactactt gacgtttaaa acgacattta
    30061 tcaataccag caaataaagc gattttacct ttattttctt cactatttaa aatagctacc
    30121 gcacctgttt gagctaacgc ttcagtaatt aatacgcctg gcattaccgc atactcagga
    30181 aaatgccctt gaaagaatgg ttcgtttcct gatacttgtt taatagccac acaacgttga
    30241 ccttcttcat attcaactac tttatcaatt aataaaaatg gctgtctgtg aggtataatt
    30301 tgtttaattt ggttataatc aaaaattgtt tccatggttg aaatcctcca ttatattaat
    30361 ttactgaatt aatcgttaat acgttcaatg tctgcaccta attgcttcaa tttgccatgt
    30421 aagtcaacat atcctctatc taaatgattc aattcagtga cacttgtttt accatcagca
    30481 actaatccag ctaaaattaa ggctgctgct gctcttaaat cagtcgcttt aacttgcgca
    30541 ccttgcaatt gacttttgcc ttcaagtttt gcactgcgac cttcaacatt gatattcgcg
    30601 ttcatacgtt tgaactctgc aacatgcata aaacgatttt caaaaactgt ttcggttacg
    30661 actttatgtc catttgctgt taataataat gccatcattt gtgactgcat atcggttggg
    30721 aaaccaggat gcggtaatgt tttgatgtca acaggctgta aatctccttc agctcttaca
    30781 cgaatgccgt cttcttgata gtccaattca acgcccattt cttctaattt atacactaaa
    30841 ctagccatat gttctttgat tgcaccacgt acaaaaatat cgccacgagt aatggcacca
    30901 gcgattagta atgtgcctgc ttcaattcta tctggaatga tagcatgttc tacaccatgt
    30961 aatgattcta caccattgat tgtaattgtg tctgtaccag caccagtaat tctaccgccc
    31021 atttcattaa tgtaatttgc taaatcgaca atttcaggtt ctttagctgc attttcaatt
    31081 aaagtcttac ccttagctaa tgatgctgcc ataataatat tttgtgttgc tcctacactt
    31141 ggaaaatcta aatgaattga tgtacctttt aatccatctt tagcattagc ataaatatta
    31201 ccattttcaa gatgaatttc tgcgcctaaa gcttcaaaac ctttaatgtg ttgctcaatc
    31261 ggtctacttc caattgcaca accaccaggc aatgcaacaa tagcatgtcc tagtcttgct
    31321 aaaagaggtc ccataactaa aatacttgca cgcattttac taacatattc atatggtgcc
    31381 tcttcattta gagtctttgt tgcatcaacg acaacagcgt tttcgtcctt tttgtatgta
    31441 acgtcagcat ttaaggttgt taatacatta tttattgttt ctacatcact taaagctgga
    31501 acattaacta atttactcgg tttatcagaa gctaataaag atgctgtcaa tattggtaat
    31561 actgcatttt tagcaccttc tactttaact tcacccgtta atttatttcc acctttgatt
    31621 actattttat ccatcgtaaa tcctccactt agaactccta ttactttaca tttccctatt
    31681 ttaaaattat tttaagtcat tatttaatta tatctagttt tgattacaaa tcatagagtt
    31741 agacttattt tattaaatat tttacctgcg tcgagtattg taacaaatct acaataaaat
    31801 tgcttactgc agtgcctaat aaaatagcaa caaatatcat acacacttgt aattgagtgg
    31861 cgtatccttt tttaaaaaat tgatctaatc taattgattg taaagcccaa taggcaatac
    31921 aaatacatac aacatgtaac actaaatgga taactgcata ttgtccgata taatccattt
    31981 tatcacctca taagattact gtattacatt gtacatgaaa aatttaaata atgatacaaa
    32041 gtttaatacc cgtttttagt aaagtttaag ctaacttact atataattct ttttacataa
    32101 atatcataac tataaagtat cttggcatga aaaaatgtag ctactttcaa aaaatgaaat
    32161 atcgactgca gatagtggtg aaggaaattt tgattttagc aatctattag tgattgtaaa
    32221 aagtaaaact attcttggga gtcggacata aatcaatgtc tagactctat agtgttatat
    32281 ttggcagtag tttgcggggc cccaacatag agaaattgga acagcaattt ctacagacaa
    32341 tgcaagttgg ggtgggccct aacataaaga aatacttttt ctttagaaat tagtatttct
    32401 tgagc
//
LOCUS       DAHHRR010000020        32353 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.020, whole genome shotgun sequence.
ACCESSION   DAHHRR010000020 DAHHRR010000000
VERSION     DAHHRR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32353
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.020"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(287..814)
                     /gene="isaB"
                     /locus_tag="NBI42_000756"
     CDS             complement(287..814)
                     /gene="isaB"
                     /locus_tag="NBI42_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HCD1777195.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKALGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
     gene            1072..1530
                     /locus_tag="NBI42_000757"
     CDS             1072..1530
                     /locus_tag="NBI42_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777196.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKATHK
                     KTETEENDQQDDSADKADKNQNNGNNVTNGNQSSTPSTVQNNGSTAPSNTQPAQPKDS
                     NSNKNNAHSGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQVQSPKN"
     gene            1770..3644
                     /locus_tag="NBI42_000758"
     CDS             1770..3644
                     /locus_tag="NBI42_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD1777197.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAEHVNVSNRTVRNDINVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLNELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIGYHFNKLDIGIER
                     IFIQSYIDRLIELMQQHIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TIIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKPRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            3721..5673
                     /locus_tag="NBI42_000759"
     CDS             3721..5673
                     /locus_tag="NBI42_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCD1777198.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINHDAP
                     IYQSETNYHSKDRDKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSVQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANMIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHILWFILAVIVGSL
                     VTMITVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTMVITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWESLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINASSTTEIYNLLMKI"
     gene            5687..6625
                     /gene="manA"
                     /locus_tag="NBI42_000760"
     CDS             5687..6625
                     /gene="manA"
                     /locus_tag="NBI42_000760"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCD1777199.1"
                     /translation="MALFLQPVFKDRIWGGYALKAFNYDIPNKTTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLAV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(6737..9718)
                     /locus_tag="NBI42_000761"
     CDS             complement(6737..9718)
                     /locus_tag="NBI42_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCD1777200.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAVRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFADITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVNLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQKALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFPKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFAQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            9929..11788
                     /locus_tag="NBI42_000762"
     CDS             9929..11788
                     /locus_tag="NBI42_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCD1777201.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDTDKADNNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDKQSTKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKGNQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNTQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            complement(12055..12615)
                     /locus_tag="NBI42_000763"
     CDS             complement(12055..12615)
                     /locus_tag="NBI42_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD1777202.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(12785..14692)
                     /gene="sasF"
                     /locus_tag="NBI42_000764"
     CDS             complement(12785..14692)
                     /gene="sasF"
                     /locus_tag="NBI42_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HCD1777203.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMVTSIILTNILPYDAQAASEKDTE
                     ISKEILSKQDLLDKVEKANRQIDQLKQLSASSKAHYKAQLNVAKTASQIDEIIKRANE
                     LDNKDNKGSHIEMNGQSDIDSKLDQLLKDLNEVSSKVDRGQQSGEDDLNAIKNDMSQT
                     ATTKHGEKDNKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDTAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDRRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKHAT
                     QSILESIFNKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYVLSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            complement(14933..16291)
                     /gene="gtfB"
                     /locus_tag="NBI42_000765"
     CDS             complement(14933..16291)
                     /gene="gtfB"
                     /locus_tag="NBI42_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HCD1777204.1"
                     /translation="MIKLLDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVKSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            complement(16284..17792)
                     /gene="gtfA"
                     /locus_tag="NBI42_000766"
     CDS             complement(16284..17792)
                     /gene="gtfA"
                     /locus_tag="NBI42_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HCD1777205.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFEDQEVIWLYQYFSDISIAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNENGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYTD
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            complement(17810..20200)
                     /gene="secA2"
                     /locus_tag="NBI42_000767"
     CDS             complement(17810..20200)
                     /gene="secA2"
                     /locus_tag="NBI42_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HCD1777206.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALLGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEKAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFGSFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVFFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSSLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENRDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQVALNRKNIQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(20190..21149)
                     /gene="asp3"
                     /locus_tag="NBI42_000768"
     CDS             complement(20190..21149)
                     /gene="asp3"
                     /locus_tag="NBI42_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HCD1777207.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQDDLEYKSMQVTKIG
                     DDQYGNDRINVIIAEPSDVYPTISNDFLKPFGHVWLVERWMDDNIEENIKQLKNDLQS
                     QDTLTAINLISYGSKSNVFATYVAQHLDCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(21127..22695)
                     /gene="asp2"
                     /locus_tag="NBI42_000769"
     CDS             complement(21127..22695)
                     /gene="asp2"
                     /locus_tag="NBI42_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HCD1777208.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVKLQYTFRLIQTGADGALIEQIILT
                     DDMLDSPLEIPTKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTVHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(22685..24238)
                     /gene="asp1"
                     /locus_tag="NBI42_000770"
     CDS             complement(22685..24238)
                     /gene="asp1"
                     /locus_tag="NBI42_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HCD1777209.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQTQNKTEFDDMISLMGMHLEN
                     NLDYQLIVLNHAPNIRTFLHRYDLYETKYWSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DNQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKLLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAHEDYYRLILLSRHQNDIPMWLRECITSVNEEYQVKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRILNTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(24249..25460)
                     /gene="secY2"
                     /locus_tag="NBI42_000771"
     CDS             complement(24249..25460)
                     /gene="secY2"
                     /locus_tag="NBI42_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HCD1777210.1"
                     /translation="MLKLLQQYEYKIIYKRILYTCFILFIYILGTNISIVSYNDMQVK
                     HETFFKIAISNMGGDVNTLNLFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKQRVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSIFNKSISDDMPMLTF
                     DSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSEVKPGKDTERYLNYQA
                     RRVCWFGSALVTIIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            complement(25589..32041)
                     /gene="sasA"
                     /locus_tag="NBI42_000772"
     CDS             complement(25589..32041)
                     /gene="sasA"
                     /locus_tag="NBI42_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HCD1777211.1"
                     /translation="MSKRQKEFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSIVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDIVSSEKSENVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSAN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTSAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGNGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSNSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTNIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTITIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIMTSTSVSTSKS
                     TSTSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSGSVSKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSVSMSTSGSLSKSQSLSTSTSDSASTSQSVSDS
                     TSNSISTSESLSESASTSDSISISNSIANSQSASASKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIATSQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSLSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSTSQSDSTSISTSFSDSTSDSKSASTASSESISQSASTSTSGSMSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSTSDSTSTSDSISEAISGSESTSISLSESNSTSDSES
                     KSASAFLSESLSESTSESTSESLSGSTSDSTSLSDSTSESGSTSTSLSNSTSGSASIS
                     TSTSNSESTSMFKSESVSTSLSTSTSTSLSDSTSLSTSLSDSASGSKSNSLSASVSTS
                     DSISTRKSESLSTSTSLSGSTSESESGSTSASESKSDSTSMSLSMSQSTSGSTSTSTS
                     ESLSDSTSTSLSLSASMNQSGVDSNSASQSASTSTSISTSESDSQSTSASLSNSQSTS
                     TSIRMSTIASESVSESTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGSASTSTS
                     TSDSRSTSASTSTSMRTSTLDSQSMSLSTSTSTSVSDSTSLSDSVSDSTSDSTSTSTS
                     GSMSASISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDSKSMSGS
                     TSVSDSGSLSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLSVSTSQRSSESVS
                     ESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESSSLSDSISMSDSTSTSDSNS
                     LSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNSQFDSMS
                     ISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGSISVSDS
                     SSTSTSESLSDSMAQSQSTSTSASGSLSTSISSSMSASASTSTSQSTSVSTSLSTSDS
                     ISDSTSISISGSQSAVESESTSDSTSISDSESLSTSGSDSTSTSTSDSTSGSTSTSVS
                     ESLSTSGSGSTSVSDSTSMSESDSTSVSMSQDKSDSTSISDSESVSISTSTSLSTSDS
                     TSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTSTSISTS
                     ESIAPNTNESQSTLSATSVSSKHDAEPAQSEERLPDTGDSIKQNGLLGGVMTLLVGLG
                     LMKRKKKKYENDQDDSQA"
BASE COUNT        10037 a         6002 c         5063 g        11251 t
ORIGIN      
        1 aaaaatgtta tctgtaacta aataaaattt taaaaattga gcttaattat attttaaaat
       61 ttatcaaggc tttatttttg tgaaaatatt aacaaacaca aaatagtgct ctatatactt
      121 aatcaatgct tgaaatatgt gagataccta tttaaataat catgatcaaa taaacatttt
      181 tatcaatttc aaacaactat gtaagacgca ataatcgtta ttatttaata aaaaagacta
      241 gaatgcggta tcactcctca cactctagcc ttaatatgtt attattttat ttacttgttt
      301 tgtatggtgt atgtccaact gttacttcga caactctatt atgatcaacg acaaaccaga
      361 tgcctaatgc ctttttagca ttttgatagt aaaaaatacc gctatcagct tcctttgttt
      421 taccattttc ttttttcaag tccttgccat aagctttttg gatatctttt aatgaaatat
      481 tatttttaac tacaaattgc aattgacttg cttcgtttcc ttttgcagaa acacctttaa
      541 aagtttgatc atatttctct acttttttta tcgtattcgt tttagctaat ttaataccat
      601 tgaatttcac attatcatac ttaatcgcgt taataaaatt cttatccaga ataaaattag
      661 cattattacc aatataacca ttataagtat aatatggggt tattgctgcc tgtgcctgtt
      721 tacttgttgg cgccgaattt tcaacaactg ttactccaat cagtgtgccc aatgctaatg
      781 ttgctgctac acaaacttta ctggttttat tcataattat ctctcttttc catacaaata
      841 ttttattcct tacactttta ttataacttt ttcagccata aaaatactaa tgattatatt
      901 tataaataca taacagtttt gttatactaa ttgaagtaca caaaaaacaa tgaaaatatt
      961 aatatcgcct acttttctaa tatttatttt ttaaaatgat tatggcaata ttgaaataaa
     1021 ttatttagct tcacgtatca actacttttc attaacatgg aggcgttata tttgaaaaaa
     1081 cttcttatta ccatcgtaat tatcgtatta atcggagcac ttgggttcgc aatttatgct
     1141 gcaataaatc attctacatc atcaagtaat aaccaagaac aagaaaataa agctacgcat
     1201 aaaaagacag aaaccgaaga aaatgatcaa caagatgata gtgcagacaa agcagataaa
     1261 aatcaaaata atggcaataa cgttactaat ggcaatcaat catctacgcc atcaactgtc
     1321 caaaataacg gttcaacagc accttcgaat acgcaaccag ctcaaccaaa agactcaaat
     1381 tcaaataaaa ataatgctca cagtggtggc agtcaatcgt caacaaacaa ccaaaataat
     1441 caacaacaga acacaccacc tgctaataaa tcaaataata ccaatacgcc ttctaaacaa
     1501 acacctcaag tacaatcacc taaaaattaa tcatttacct ggtacgtcga caagtatcag
     1561 gtacataaat tcagaaaaac agcagtaaga tatttttcta tttgaaatat cttactgctg
     1621 ttctttattt gcataattcg cactctgtta aaatcatata aaagttcgct ttaatgtcat
     1681 tgacgtatgt ataagctctc atgtttaata atattaatac acctttaaaa acgcttacac
     1741 gaaaaattta gaaaggaggt acatgttaaa tgcttgatag acacttaaaa ctactccaat
     1801 tttttattaa aaatccatca aagcacatta gttctaatga aattgctgaa catgtcaatg
     1861 tttcaaaccg cacagttcgc aatgatatca atgttatcaa tagcaatttt atggatgata
     1921 tcatcgtaag tattaaatct aaaggatatc aattaaatac atcgcagtat accttagaaa
     1981 cgataactga acgttacacg catattcaat catataaaga aaaactatta ttatctatgg
     2041 cgtatcaact gctaatgcac aataaatcgc agacattgca acaactagag caagattatc
     2101 tattatctaa aaccgtgctc aacgactatt tcgttcgaat tcaacaatgg tgtcaaaaat
     2161 ttaatatcgc tttaacaatc aagaaaaaac aaggtattgt ggtcgatgga acagataatg
     2221 atatcactaa tgccatcatc catttaaatc aattatcatc agggcatgta catgttgaag
     2281 acctaatttt aaatgaatta cctgattcac accaaaggat gatttcgcat attatacaag
     2341 agacacttca acaacatagt attgaaacaa ctgatattca aatacaacag cttttaattc
     2401 acctcatttt aatcattaaa cgtaagcaat ctttaaatga gctagacacg ttaaaccacg
     2461 attcaaaatt gatatctcaa tcttgcatta agcaaatcaa cgaaagatta ggttatgact
     2521 tgaacgaaaa agtaatcaat atgttttcat tctttattgg ttatcatttt aataaactcg
     2581 atattggcat agaacgtata tttatacaaa gttatattga tcgcttaatc gaattgatgc
     2641 agcaacatat tcatgtacct ttcaatgaag attcgatttt acaacaaaat ttatataatc
     2701 attttagtaa agcatattta cgtatcactc aaaacattta tctaaacaat cctttagtta
     2761 tagagattaa aaaactctat ccctttgtgt ttaacacact ttttgaagcg attgataaat
     2821 tagcaattga tactgatata gagatgagcg aagatgaaat tgccttttta acaattcatt
     2881 ttcaagctgc catcgagcgt cgtaccaaaa cacaattaaa tgtagtcatt gcttgttatt
     2941 atggtctagg tgtgtcgaat tttttagaaa ctaaaataaa taacttatcc gaagagttgt
     3001 cagtgataaa tacgattaaa ctagaaaata taacacacta tcactttgac aatgtagatt
     3061 tattaataac gacccatgat attccaaaac aaacgctaaa catacttcct aagcacctaa
     3121 cgattatcaa ggttgcacca ttattttctg aagatgaccg tcacaaaatc agacacgttg
     3181 tgaagcaaaa gcaaaatccg gttcaagcac atcatcatat ggacactgtc aatttccttg
     3241 tcgtaaatac tgaacaaaaa ccacgacata ctgtgcaaat ttttgaagaa gctcaaaaaa
     3301 tattacaagc acatcatgcg attgtagaag gatatatcga atcagcttta gagcgtgaga
     3361 aatcatcttc tacatacata ggtaatttta tggcgattcc tcatggagat cctgaaaaag
     3421 ttttacaatc acatgtgctt atttttcgca caaaagatgt ttttccttgg cgacaacacg
     3481 atgtcaaact tgtctttttc ttagcgattt cgcataaaga caaagctttt acaaaacaaa
     3541 tgatgcaatt aattgctaac ttggatgatg attcagtgaa ccatttatgt agtttagatg
     3601 atcatagttt aaagcaacag ttatttgaat acttacaaga ataattgcca ctagtaacgg
     3661 taattattct tttttttagt atcgagaata cgcttacaat atagaaaagg agatgatttt
     3721 atgaaaattg tagctattac ctcatgccca aatggcattg cacatacata tatggctcaa
     3781 gaaaaacttg agcaggtagc aaaagaaatg ggtgtggata ttaaagtaga gacacagggc
     3841 ggtgttggtg ctgaaaatgt tttaactacg caagacatcg aagaagctga cggtgtcata
     3901 attgcagctg ataaacaagt tgatctgtcc cgatttgttg gtaaacggtt gattaatgaa
     3961 aatgttcgcg aagggattca taatccgaga ggtctaattc aacgtatcat taatcatgat
     4021 gcgcctattt atcaatctga aacaaattat cattcgaaag atcgcgataa gtctaaaagt
     4081 ggtattcaaa tggtatatca acatttaatg aacggtgtat cgtttatggt tccttttatc
     4141 gtagttggtg ggctccttat cgccatcgcg ctgactctag gcggtgaaac gacatcaaaa
     4201 ggattagtca tcccagatga ttcattttgg aaatccattg aaaacattgg tagtttagcc
     4261 tttaaattta tggtcccaat actagctgga tatatcgctg ttagtatcgc tgacaagccc
     4321 ggtcttgttc ctggtatgat tggcggtgct atcgctgctg acggtagttt ttatggcagt
     4381 gatgcaggtg caggattttt aggtggtatt gttgctggtt tcttagcagg ttatatcgca
     4441 aagtggatta aagatattaa agttccaaaa gcaatggcac caattatgcc tatcattatt
     4501 attcccatta tttcatcggt cgttgtagga ttgatattta tatttttaat cggtgcacca
     4561 atttctaata tttttgaggc attaacaacc tggttaaaaa gtgtgcaagg tgctaatatt
     4621 attatacttg cgatgattat aggtgcgatg atcgccttcg atatgggcgg tccagtaaac
     4681 aaagtagcat ttttatttgg ctcagcactt attgctgaag gtaattacgc cgttatgggt
     4741 atggtcgcag ttgctgtatg tacaccacca atcggcttag gtttagcaac gtttgttcaa
     4801 aagtataaat tcaatcattc cgaaagagaa atgggtaagg cttccttcac tatgggacta
     4861 tttggtatta ctgaaggtgc tattcctttc gcagcgcaag atccactgcg cattatacct
     4921 gctaacatga ttggagcgat gattgcatca gtcattgcag ccatcggtgg tgttggagat
     4981 agagtggcac atggcggccc aattgtagcc gtattaggtg gtattgatca tatattatgg
     5041 tttatcttag cagtgatagt aggtagtctt gtcacaatga ttactgtact attattaaaa
     5101 cgcaatacac cggttatagc agtagacgca ccagcgcaac acacgcaact tcatgataca
     5161 gatataacac aacatgatac agaggttgac aatgtagata acaccgcgaa acattcacat
     5221 ctcaataaac cttctcatgt ttttgatcaa caaactatgg taattactga tcacgacatg
     5281 tctcgaaacg aagcaattga tatgttgatt cataaactta aaatttgccg ttatgtagag
     5341 cacacatcgc atttaaaaaa tgcaatacta gaacgtgaaa tggagtctac gacagctatt
     5401 ggcatgaacg tggccattcc acatgcaaag tctgatgttg taaaacaacc gattgtagct
     5461 gttatgaaaa ataatcatgg tgttaaatgg gagagtctag atggtagtct acctcaattg
     5521 atattcttaa ttgccgtacc aaacaacagt caggatacac atttaaaaat acttcagcgc
     5581 ttatctaaag cgttaatgaa tgatgagaca cgccaatcat taattaatgc aagcagtact
     5641 acagaaattt acaacttatt aatgaaaata tagaggagtt ttaactatgg cattattttt
     5701 acaacccgtt tttaaagaca gaatttgggg cggttatgca ttaaaagcat ttaactatga
     5761 tatccctaat aaaacaactg gtgaatgctg ggcaatttct gcacatccaa atggtcctaa
     5821 tacaattatc aatggtccat acaaggatat gaccctagat caactttggt cacaacatcg
     5881 tgaattattc gataatgatt cacgagatag tttccctctg ctaactaaag tattagatgc
     5941 caatgataaa ttatctgttc aagttcatcc ggatgatgat tacgctttaa aacacgaagg
     6001 cgaactaggt aaaacagaat gttggtatat tttagatgca gccccaggtg ctgaaattat
     6061 atatggcgta catgcacata ataaacagac gttaatcgac atgattgata atcatgaatt
     6121 tgacagtctt ttcaaacgta tacctgttaa gcccggtgat ttttattatg ttcctgctgg
     6181 cactgtccat gccattggtt cgggtatttt aattttagag acacaacaat catccgatac
     6241 gacataccgt atttatgact atgatagacg agatcaaaat ggtcaattgc gtgatttgca
     6301 cttagaacaa agtaaagatg tgattgaatt aggcaatcat gaaccaaata ctactcctat
     6361 aacaactcac atcgacacac acactgtaac tcaatttgta gataatcaat attttgcagt
     6421 atataaatgg gatattcatg gagatttaaa attcaataaa ccacatgctt attgcttagt
     6481 tacaattatc gatggtactg gcactttagc agtaaacgat aagcactatg acatccaaaa
     6541 aggttctagt tttattttaa ctactgaaga cgaggatatt cactttgtcg gtaatattag
     6601 tgctatagta agtcatcctg gataatagca atgataacac ccgtcccata aaagacagaa
     6661 attactatct cttctgtcta aaaatataaa aatccacaca ttaaggagct ataaccctaa
     6721 tgtgtggatt taatttttat tctgtaacgt tactttggtc aactttttca gatacgcgct
     6781 tcatcaatgg atctgttaca ggttttaaaa ttaaacctac aacgaagaat cctatgccaa
     6841 ataacgttaa tataattaat tttgtaatta agatttccgg aacaataccg cctactgttt
     6901 cacgtaatga atcaattgca tacgtaaatg gtaagtatgg cgaaatgttt tggaaaaatt
     6961 gtggcgtcgt ttgaattggg aatgttcctc caccacctgc aatttgtaat acaagcaata
     7021 cgattgcaat ggctttacct gggttaccaa gtaatgatac gcacgtatat acgattgagt
     7081 tgaaaataat cgaacagaaa atcgttataa gtacgaataa tacaggtgac tcaacccctg
     7141 cttttaggat taacaaatct ccaaccgata caatgagtgc ttgcaacata ccaagcatta
     7201 taaagaaccc tgccttacct aagaacactt gtcttgtcgt taagactgac tctagactct
     7261 tatgtttatt atcaacagtt aataaactta ccatcaagag tgcacctacc catactgaca
     7321 gtgcagtata gaatggtgtc atacctgaac cataatcctt aactgggaaa acatcaacag
     7381 tcttcttatt aataggattt gcaataatac ccgcttgctt tttcaaatca ttcttaagtg
     7441 tgtcgattaa tttgtcgact gcatcatctt tgtctaattt cttgaaaatt ttattcgctt
     7501 ttttcagatc tttttcaaca tctggcaatt tattttgtgc aaaatcagct aaatctgata
     7561 aagccttttt cgcttgtggc tgatttttat ttagtaatcc taccgcttga tcataaccat
     7621 ttaataacgt tggtaaattt ttattcacac ttgcagtcgc attggttaaa cgttgctcca
     7681 actgtggtaa atcgttacgt acaaagttag cagctttagc tacatcactt ttcactttcg
     7741 ggaagtttgc ttgagcattt ttaacagcac taatgtattg ttgttcaata cttggtaata
     7801 ctgtttgtaa acgatcgata cgtgttttac cattttttaa aatagcttgt ccactgttaa
     7861 tgacttgatc aattgtattt agcttagact gaacattaga caatgccttt tgccctttat
     7921 tcaattcatc cataatacgt tgtgatattg atactaaaga gctgttaaga tcttttttaa
     7981 cataatctct aaatgatgat aatgatgaat ctagttttga cagttgatct agtaatttag
     8041 tagcttcagc agttcctgaa ctaccattct ttaatgcatt gcttaattga ttaactaacc
     8101 ttaatgattc attaattcga ttattagctg cctttacctt atcaatttct tttgataaat
     8161 taacattttc attcttctca atctctttta atgtatcgat aaattgttga ttgtattgag
     8221 ttgtgtattc taaaccggac ttaacattat ctaaagactc tctaaaatca gaaggcttgt
     8281 ccgatgctaa aatgccatac gaaatattac gtaatgcctg actatctttt tgtgcggctt
     8341 tcgcttgggt atctaaatta tttgataatg ataataaaga gcccgttaat gccgcttcca
     8401 ttgatttaac atctttatct gtaatgtttt gtgtgttctc gttagctgtg ctgacttgtg
     8461 ttgttgttat atctgcaaaa ctttgaccat cttttttccc gcttgtactt tgtggtgtag
     8521 tcgatacagg cgcattatta ctatttggtg cggtattttg cgtaacatta ttatttggtg
     8581 cagtatcgcc acttggtgca cttggtggcg ttgatgctac accattacca gctgcaggtg
     8641 cactatttga tgtttcgttg ttcgccgcgc tattttgagg aatctgttgc tggtttcttc
     8701 gatttaaatc ttgcgcgcgt cgaactgcat cttgataatc accgactcta cttctaactt
     8761 gtgtgacgaa gcctttggca tcatttaact gtccattagc ttgatcaata ccttgactcg
     8821 caatgttaag tccttgatta attttaggga actgcgctgg cacgtcatca gctgcaaaat
     8881 ttaacgcttt ttcaattttc ggcatataat tatttaaagc taatatcaac ttagcctttt
     8941 cattaagttg cggaatagca ccattgactt cgtttaattt tttctgagca tctaaaatat
     9001 cacctttata atttcctagt tttctaaaat cattggcata tttatctaaa tccgcttggt
     9061 ggttattcaa atatacaatt ttattcgcaa agtcattaat cttaggtaaa gctttatctg
     9121 ctgaatatac cgcatttttt atcttattaa ttgtcggcac attttcttca atagttaaac
     9181 ctgctttgtt agcttcttct aataatgctc gagttactgt tttattaaat tgttcattcg
     9241 ctttttcgac gacaactgac gaaccagtat ctgttagctt agacgcaaca gcgttaatct
     9301 tctgattcac cttaaattct acatctgctt tttgaggctg cttacgtagt gtgccggtaa
     9361 tttcatgtgt aaacttagat gggatgtaaa tacctgcaaa atatttaccc atttttatct
     9421 catgatcagc tttctctcta cttacaaact gccaatcaaa acttttattt ttcttgagtg
     9481 tattaaccat cgtattaccg acattaactt ttttccctct gattgtgtcg cctttatctt
     9541 cattaacgac tgcgaccttg atgtgtcccg tgttgccata tggatcccac attgcccata
     9601 agttaaacca agcgtagaac gatggcaaaa tagctaagcc tgctaagata atccacacag
     9661 ctggcgtctt agctactttc ttcagatcca ttttaaataa tttaaatgcg ttcttcattg
     9721 tcacactcct atgtaggaat tattcatatt ttttatatat tttttgtaaa ttaatttatt
     9781 tttgcgttgt gaattagtat aatcaattta ctggaagata tttagtcgat tgatacctat
     9841 caactatttt cagcatacga taaattataa caaatcatag tttattatca cacttaatta
     9901 ttatattttt caagggagaa tacgaaatat gcctaaaaat aaaattttaa tttatttgct
     9961 atcaactacg ctcgtattac ctactttagt ttcacctacc gcttatgctg atacacctca
    10021 aaaagatact acagctaaga caacatctca tgattcaaaa aaatctaatg acgatgaaac
    10081 ttctaaggat actacaagta aagatactga taaagcagac aacaataata caagtaacca
    10141 agacaataac gacaaaaaat ttaaaactat agacgacagc acttcagact ctaacaatat
    10201 cattgatttt atttataaga atttaccaca aaccaatata aaccaattgc taaccaaaaa
    10261 taaatacgat gataattact cattaacaac tttaatccaa aacttattca atttaaattc
    10321 ggatatttct gattacgaac aacctcgtaa tggtgaaaag tcaacaaatg attcgaataa
    10381 aaacagtgac aatagcatca aaaatgacac tgataagcaa tcaactaaac aagataaagc
    10441 agacaatcaa aaagcaccta aatcaaacaa tacaaaacca agtacatcta ataagcaacc
    10501 aaattcgcca aagccaacac aaccaaatca atcaaatagt caaccagcaa gtgacgataa
    10561 aggaaatcaa aaatcttcat cgaaagataa tcaatcaatg tcagattcgg ctttagactc
    10621 tattttggat caatacagtg aagatgcaaa gaaaacacaa aaagattacg catctcaatc
    10681 taaaaaagac aaaaatgaaa aatctaatac aaagaataca cagttaccaa cacaagatga
    10741 attgaaacat aaatctaaac ctgctcaatc attcaataac gatgttaatc aaaaggatac
    10801 acgtgcaaca tcattattcg aaacagatcc tagtatatct aacaatgatg atagtggaca
    10861 atttaacgtt gttgactcaa aagatacacg tcaatttgta aaatcaattg ctaaagatgc
    10921 acatcgcatt ggtcaagata acgatattta tgcgtctgtc atgattgccc aagcaatctt
    10981 agaatctgac tcaggtcgta gtgctttagc taagtcacca aaccataatt tattcggtat
    11041 caaaggtgct tttgaaggga attctgttcc ttttaacaca ttagaagctg atggtaatca
    11101 attgtatagt attaatgctg gattccgaaa atatccaagc acgaaagaat cactaaaaga
    11161 ttactctgac cttattaaaa atggtattga tggcaatcga acaatttata aaccaacatg
    11221 gaaatcggaa gccgattctt ataaagatgc aacatcacac ttatctaaaa catatgctac
    11281 agatccaaac tatgctaaga aattaaacag tattattaaa cactatcaat taactcagtt
    11341 tgacgatgaa cgcatgccag atttagataa atatgaacgt tctatcaagg attatgatga
    11401 ttcatcagat gaattcaaac ctttccgtga ggtatctgat agtatgccat atccacatgg
    11461 ccaatgtact tggtacgtat ataaccgtat gaaacaattt ggtacatcta tctcaggtga
    11521 tttaggtgat gcacataatt ggaataatcg agctcaatac cgtgattatc aagtaagtca
    11581 tacaccaaaa cgtcatgctg ctgttgtatt tgaggctgga caatttggtg cagatcaaca
    11641 ttacggtcat gtagcatttg ttgaaaaagt taacagtgat ggttctatcg ttatttcaga
    11701 atccaatgtt aaaggattag gtatcatttc tcatagaact atcaatgcag ctgccgctga
    11761 agaattatca tatattacag gtaaataagt attatattaa acccgtaaaa tttataagta
    11821 taaacaagga gttcggactt aaacatattt ctgttcatag gtccgatttc ttattcaatt
    11881 aaacccgagg cattcagttc gaacgcctcg ggtcatttta tataaatata ttattttatg
    11941 ttcaaatgtt cctcatcata tccgtttcaa ttgccatctc acacatttta taaatatgag
    12001 caaatgtact tattctctaa cattactgcc gagctttaat tgacgttata ttaactacaa
    12061 actacttttc catgactcta cggattcaat gtcacatgag cgtgataaaa tttgttcaat
    12121 aataaagtca tgtttatcat ctgatctatc accaacagca tcttctaaaa cagtaatata
    12181 atagtcttta tctacacttt ctaatgccgt gctcaataca gctccacttg tagagacacc
    12241 cgttaatact aaatgattaa tatcatttgc acgtaaataa acttccaagt aactacctgt
    12301 aaatgcgcta aagcgtcgct tagaaataat cggctcatct tctagtggtg ctaagtcttc
    12361 aagtattcgt gtagatgcat ctgcttcagt aatcgcatat ccttgagctt taattgttga
    12421 aaatacttta ttactcgagg agacatcatt aaaatgctta tctaacacta aacgtatgaa
    12481 aatgactggt attcgatgtt gtcttgctgc ttcaattgct ctctgattcg ctttaataat
    12541 attttttatt ctaggtacac tactcgctat accttcttgc atatccaaaa ctaatagcgc
    12601 cgtttttcga gacatcttca ttctccttta cttgtgtagt tctaagtcgt taaattcatt
    12661 ataacgttaa aatgatggac aatctattca ttgcattttg catatacttc acaataattt
    12721 aagggggaaa taagacgtct tatatactta aaaaaaatat atagatgctc ttcccccaat
    12781 ataattatgc tttatttttc aacttattgc gtcgtgataa ccaaatcatt agtacaccca
    12841 ttgcaccaac aattacagat atcggcaacc aatgttcttt tatcgtttcc cccgctttag
    12901 gcaaagatac attaccatca gcatttaata atccacttaa caatccatta cctttaccaa
    12961 gtgttaacgt cttttctggc tttggtgtgg gtatatctgg aatactgtct aataaatttg
    13021 atccttgatt cattaaattt gctaacttat ttaaatctgt cgttttccca tttttattca
    13081 atctatctag taaacttgga cgatttacta ttggtgataa aacatagtct atatcttttt
    13141 ttgtttgatt gagtctcttt tgtaaattca ataaatcatc cgctttacca ttcaatgccg
    13201 atttaactaa attaaaaatt ttattttgat ctgtttctat tttagtaatt aaatctgcca
    13261 gtaattttgc cttttgtctt tctatacgtg ttgctaaaat cgtttcaatt gcttgttttt
    13321 tatctttggc attattcaaa attgctttta atatatcatc tgaagacgtg tcgccagttg
    13381 atgcaaaatg tttcttcaat tggtcaacga tttggcgatt tgataatcct ttattcgtcc
    13441 aatctttagc caatttatct gcttcagctt ttcctaattt cgtttgtaaa atttgagaaa
    13501 tcaatagcga cttatcttgt gattgatcaa tcaatgacgt taataaatca tcactcgttg
    13561 tcagagatag ttgatcaata tgacgagtaa tttgatctgc aatttgttga tctgttttac
    13621 catcaacacg tatatctttt agaattttat ctgcctcgtc tttattaaat atactttcta
    13681 aaatgctttg tgtagcatgc tttttatcat cagtacgtgc aagttcttcc aaaataatat
    13741 ttcgttgact ttttatacgc tctttcgtct tatttacttc gctcattaag tctgattttt
    13801 gatttttagg aagttgcgta tttgcaatac gtcgatctaa agattgtaac gtattcagtt
    13861 tatgatatgt gtaatgttgc gttgaggcat tacttttagc caatttttca atcatagcat
    13921 gattaatttt atcgcttcct tgtaatttat cagtgagtcg attactatgg ctttgattct
    13981 cttcatttga aagaaattta tttaacacaa cgtgtccaga accatcatta tttggcgttt
    14041 tagctacttc atgattatta tctgttgtag acactgctgt atcttccgat gtatctttcg
    14101 atgcatcttt cgatttgtgt atttgctgat tcaaatgatc taagtcttct aacgccttat
    14161 ttaccattgc ttcatcattt ttattatctt tttctccatg ttttgttgta gccgtttgtg
    14221 acatatcatt ttttattgca ttaagatcgt cctcgccact ttgttgaccc ctatcaacct
    14281 ttgaagaaac ctcatttaaa tctttaagca attgatctaa tttactgtct atatcacttt
    14341 gaccgttcat ttcaatgtga gaacctttat tgtctttgtt atccaactca ttagctcgtt
    14401 ttatgatttc atctatttgc gatgctgttt tcgctacatt tagttgtgct ttataatgtg
    14461 ctttagatga agccgataac tgttttaatt ggtcaatttg acgatttgct ttttcaactt
    14521 tgtctaataa atcttgctta gacaatatct cttttgaaat ttcagtatcc ttttcagatg
    14581 cagcttgggc atcgtacggc aagatattcg ttaaaatgat actagtcacc atcattgtcg
    14641 aacacgataa ctttacatat aattgaaacg gtttccctcg atatttagcc atcaacatac
    14701 tcctttctca cttacttcct taaaagaatt acatactatt atatacctgt ttataagaaa
    14761 tttacactta tctaactagt tattgttgtt agtaaatatc cacttattac ttagcttata
    14821 tttaagtaaa caaaaaagca tgacgtaata tcatattgtc catgtcgcta acatcatatt
    14881 acgtcaaatc ttttatatta aatgatgctt tattttagac tgctttttct ttttagcttt
    14941 cgagcgcctg tttaaaaact tgctcgaatt gttcacgcga gatttcgtgt gcatgtgctt
    15001 tttgtgctaa taaagcatct cgaaactgtt gttgatcttt caaactttct aacatttgta
    15061 ttaattggtc tttactttcc attgttatct catcattatg ctcaaataag tgctctgata
    15121 gcgttacttt agcatggtgt gctgtttgac gataacctaa aatcaataac tcatagtcaa
    15181 acgcttgttc caccgcattt aaaatttcat taccctcatt aatatcaaga taaatatcac
    15241 ataactggta tagttcattt accctgtcta tattaataga tgggtataag tgcacattag
    15301 catattgatc aagttgcatt agcttatcag acatctctgt aatagcagcg atgtgaaact
    15361 taaaatctgg taaagtttca accaatacct tgatgttacg aagttgatcc gagttagtta
    15421 atattacaat ttctttagta tatctattac gactacgata attatataga tatccgcctt
    15481 gtaaaatacg agatttaacc tttgcgtctg ctatattgag catcgtttca tattcgtttt
    15541 tatctggaat aataatatta caatgtcgtt tcatatcacc tttacacatc aattgcatat
    15601 ttcccgggac attaccatta cagtgttctt gccataccaa aacatcacta ccttttgatg
    15661 gcaaattata taacactgaa aatggtaggg ctagtgagtt aataacgaaa tgatgttccg
    15721 taatttcaag ttgcttgata aaaaataata cgaatgcgag ctttgaaggg aaaaagtaag
    15781 acttcccttg ccaatccaat atgacatcag atgttacaaa attttcataa atcacttctt
    15841 taccttctgc tgtcatatat ttcttcaaga tcgctttacg atttaaatcg taaacagttt
    15901 gtgcaaattt aataccattc ttagaataat aatcgacaaa tcggacacgt tgttggtcat
    15961 caaaccattc gacacgacta acaattctag gacgctctcc actttgataa aatattttgc
    16021 ctcgtagacg tcccatatca ttaattgtag ccgaattgtt gttaccttta atttcccaaa
    16081 aagctggtac agtaacctga ttaaaaaatc gtggtttcat attttctgta ttatgattat
    16141 ctgcaaaaaa ttgatacggt gatataacat cgtccggtaa aaagccattg tcattgagta
    16201 caattgttaa atcttcttcc aacttactgg ctttaaaaga ctcatataac tttcgtgaat
    16261 gatcgttaaa gtaatcaagt aatttaatca tgtagcacct cttgaactaa tgtttcccat
    16321 tttaaaataa tatcttgagt cataaattgc tgtgccactt cataagagat gtcatgtggt
    16381 gtctggggac cattgttaaa atacataaca atggcatgag ctagttttgc gataacatca
    16441 tccacactat cttcatcggt atcaaaaggt accaagtagc cattttcccc atctcgaata
    16501 aaggttgggt taccataatt cacatttaat ccaatcatac ctagtcctga gcctaccgct
    16561 tccattagtg ttaacccaaa accttcgcta gttgatgcag aaagaaataa ctcataatca
    16621 gtatagattt catcaagttt aacatgccct agtaaacgaa tataatcttg tgcgcggtgt
    16681 gtatcaataa ttttacgcag tcgcgtcttc tcgctacctt ctccataaat atcaaatgtt
    16741 aattctggca cttgtcgttt agccacgata accgctttga caagccaatc aatatgtttc
    16801 tcatttgcta aacgagatgc actaatcatc gcatatggct ttcttgataa tgttggatat
    16861 gataatgcat caatgcttcc caccggaata gtataaacac gtgggcgata accttgatat
    16921 tgctcaaatt gtcgacaaac catatgattt tgaatatctg ttgctgtaat aaagaaatca
    16981 atgtatttag cttttgaaaa ttgatattca taataattgt tccatagtat atgctgctcg
    17041 ctcatcatat tattactata atgatcagca tgaatcacaa caccaacttt actatcacct
    17101 ttatgctgca aaacagcctg accaatatca gaagcgcggt ctaatatgac aatatcgtct
    17161 cgggttaaat tcaatcgttg taaaaagtat gcaataaatt ccgttttgtt atacaacacc
    17221 gcatcttcaa acacatatat agagctgtct ccatcaatat attcgttata agcgatggag
    17281 ccattttcat tatagaattg tcgcatatat aatttcgctt tattatcagc tggtgcataa
    17341 tactcagaaa atatgcgtgt ataactataa aaatctttac gtactaacat actattaatt
    17401 acaaattctg cacgatccac aatgtctttt tgttcatttt gcagataaca tgttacaaat
    17461 gatgatttcc cattaaaata taggcggact atcttaccat ttctttctct aaaactaata
    17521 tcatgaccaa gctcacgttc aatgtcatct aacgtgtacg ttgttggtgc tatagaaata
    17581 tcactaaaat actgatacaa ccaaataact tcttgatctt caaacccaat gttttgcgtt
    17641 aatgtctgta tgttctctga ctgtataaaa tctaaaaaca caaatttagt gtcttgattt
    17701 gtacgtctca ataatttagc acggtaagct tgtgcatatt caacaccgct actcgcccag
    17761 cctataccaa agtttatatt atatattgtc atgcgctacc ccttttcatt tatggaaaat
    17821 gtataactgg cataccctct ttatcaaatg taatcatgct ttgacaaata tttttcacca
    17881 ttcttttttt gatatttcgt gtcataactt caaatgaatc taatgcaact ctatggtatt
    17941 caaaaatagc gttacgttgc ccattttgtc gttgattaac gcttgctttt aattgttgta
    18001 aatagtcgac ttgttctaac caacatgaat caattgcttt caaaaagact ttttgaacga
    18061 aaatattata ataatatgca ctttgtatat ttttacgatt caaagctact tgcttttcaa
    18121 attgctctaa taaaaatgtc actactgctt gcttatcttt aaaattaaca caagccacat
    18181 ctttattaaa ttggaaactt aaattttgat aaatatactc gacaacacgc gattttgtta
    18241 gcaccttttc ctcatttaca aacatttcaa atacatcttt agctaacgct ttaaaatctc
    18301 gattctcagc atcatctatt tctaaaactc gattgcgttc ctcgtataca agatctcgct
    18361 gtatactaat gcttttttca aattcattag ccatttcacg agctttaacc ccttgttctt
    18421 ccgagatacg ctgcgcttta actacaattt gcttaacttt gcgattaaac aaactacttt
    18481 gcgataatcg ttgtgcatct aatgaatata attgattatt ttccgctaaa ttactatcgc
    18541 tccatcgctt aactaaataa tcatctagtg aaatatatat acaagatgat cctggatccc
    18601 cttgtctacc agaacgacca cgtaattgcc tgtctacacg gctattttcc atatgctcat
    18661 gaataataac agctaatcca cctaatgctt cgacaccttc accaagttta atatctgtgc
    18721 ctcgacctgc catactagtc gcaacagtca tggaaccaat ttgccctgct tcagctatca
    18781 tctgcgcttc ttttgcaaca ttttgcgcaa tgagtaaatt attaggaata tccatttgaa
    18841 aaaatacttt tgaaaagtat tcagccgctt cagcagttct cgttatgagt aaaaccggtc
    18901 tccctgtttc atgaagttca actatatcat gaatcattgc gatgtttttc tcatcaactg
    18961 aacgaaacac tttatctggt tcatcgatac gttgaatcgc tttatcagtt ggtacttgta
    19021 cgactatttt tgaatacaaa tcaaagaact ctgattcgcc taattttcct gtagctgtca
    19081 tacctgaaaa tgatccaaaa agtttaaata aattctggaa ggtaattgtt gccataacac
    19141 ttttatctgt tgaaacctcc ataccttctt tcgcttcaat agcttggtga agtccagctt
    19201 gcaacttagt tcccggtaac atacgacctg taatacggtc aattaaaaca atatcaccat
    19261 tatatacaaa gtaatcgaca ttagattcaa acaaatattg cgcgcgcagt gctagattaa
    19321 tattacgcac taggaccatc gctttttcgc tatataaatc ttcaacatta aagtatgatt
    19381 gtgccgcttc aatgccttga tttaacagcc atatttcttt tttggtcttc ttcattttaa
    19441 aatgcacgtc ttcaatcaat gtatctacaa actctttcac aatatgaaat agatttgatt
    19501 gtaatcttgg tgcgcccgaa atgactaatg gtgtttgagc agcatctaaa atgattgaat
    19561 ccacttcatc aataataccg taatttaatt gtggtaaaaa ttttccttcc gcactatctg
    19621 ccaaattatc aattaaataa tcaaaaccga gacgtccatt agttgtatat ataatatcat
    19681 gttcatatat attacgtttt tccccttttt gatactcata atccacaata tcaacaaaac
    19741 ctaatgaagc agttaaacct aaccattcat ataatggttg catctcttca aaatcacgct
    19801 tcgctaaata atcattcgtt gtaattaaat atgttccttt tcccaaaagt gcatttaaat
    19861 ataaaggcat cgttgctgtt aatgttttac cttcacctgt ttgcatctcc gcaatgttac
    19921 cttcatgcaa tacaatcgct ccgattaact gaacttcttt aggatacata cctaatactc
    19981 tccagctcgc ttcacgtgcc actgcataag cttcaggtaa caatgtgtct agtgtatcaa
    20041 ctcctgatgc taaacgttct ttaaattcta ttgtcttttg ttttaacgca tcatcagaat
    20101 atgatttaac ttcatcgctc catgtattga tgcgtttaac tatttttcta atcgacttta
    20161 gtcttaattc gtttatcgta acatctagtt tatgtttcat ttacttcccc accattcagt
    20221 ttcgatacat ctaagtaatc taaaaatcgt actggattca ttaaacgtga catataattt
    20281 agatgtttgt cttgctcttc tttaaaataa acctcgacat ttgtatcttt tagttcatga
    20341 tttcctggga catgttctgt aagccatcct tttaaatcat catcttcatg gcttgtgcga
    20401 tacactttgc aatctaaatg ctgagcgaca taagttgcaa aaacatttga ctttgaccca
    20461 taactaatca aattaatagc cgttagggta tcttgacttt gcaaatcatt ctttagttgc
    20521 ttaatatttt cctcgatatt gtcgtccatc caacgttcaa cgagccaaac atgaccaaac
    20581 ggtttcaaaa aatcattcga aatagttgga tagacgtcag atggttctgc aataatgaca
    20641 ttaatcctat catttccata ttggtcatcg cctatcttcg ttacctgcat gcttttatat
    20701 tctaaatcat cttgatgcgt catctctgtg attgttaaac atctaaatat aagactcgtc
    20761 gatgctgcat tcatcatttt tattttataa gcataggctt catcaggata ttgaatcgta
    20821 atactatttg actttacaat ctcagtactt agttttgttc catttttatt ataaaaaatg
    20881 atgataaaat acactgaacc agcaggcgtt gcatcaaaat caaaatgcaa tttataatgc
    20941 tgtcctctac gcaaaattgg taaacttggc gcacttttat attttgaaaa ttgttttaac
    21001 atcaaccact catgaatcgg taatccagag ggcatcaaag gatttataaa agtcacttca
    21061 ccatttgaaa atgatacttt tgagccatac ataaatgtag tttgtgaaat ataattccaa
    21121 gtaactttaa atgttttgtt tttcagcatg ttgaactctc ccaaacttgt cttccaaaat
    21181 aatgttgtaa aaattaacaa accaacttgc aatggtaggt gaatcatcat tatgtcgccc
    21241 aggaatactg cgattcatca ctcttgcttg gtgtactgtc aatacaggta gtagctcttg
    21301 aaatgcatgt ggatcataat catcatgttg catatatgct atggcaaaaa cagtttgtga
    21361 caatgatttc ttttgaaatg tttgccaaaa tttttgattt aatgcctgta tcgacgcttg
    21421 agatgtatca ccttcattag acaccaggac gtctaatgct gtaccgaact cttctggtct
    21481 aagtaatcgc atatgttcag caatcgttcc aatattaaca agtggcttac caacaataat
    21541 tgcctgagga tttaactgag caccataata caaggcacca aatgacccca tagataaacc
    21601 agataatatt aattcatgtg atttgaaatt cagcttttct aatgtctcgt caataacatt
    21661 gataatacct tgttcgtatt cagatgaacc gatataaaag ctaccacctt caacacgagg
    21721 atcgccgata agtaaaaacg gtgcattcat acgtttcatc atataatatc cttcgaaacc
    21781 ttccgctgtt cgataaccac taaaatatac gtttagtggc ggtttcatat caccagggtg
    21841 gaaataataa ataaattcct gtcgttgact atctacgaaa cgactaccac caagtaaaaa
    21901 ttgacccatg tctaatctag accatcgttt gtgtataggt cctaaatgta ccgtcccgtt
    21961 gccacgcgcc ttaacagtta cacttatata agcatcaaat ggtttcgtag gtatctctaa
    22021 aggactgtct aacatatcat cagttaatat gatttgttca attaatgcac catcagcgcc
    22081 agtctgaatc aatctaaatg tatattgcaa cttgaccgca ccatcaatat caaattctgg
    22141 ccatatttga ataactttat ctttatcgta aacgagatta ttttgccaag atgcgatagg
    22201 tttaaattct tttccaaatt ctccactcaa cgtgagctct gaattacctt ggtaaacgac
    22261 atctccttta aaattcggat gaacaagtgc taacttagga gaaaccttat ctccatactg
    22321 tcctgagaag ctaactgcct ctaatttatt attacgttct tcaatgttcc ggtaatgtaa
    22381 tggttgaaca acgtattttt ggacattttc gtcttgttca tattcaacag accaaaatga
    22441 ttcatcaaca tacgtattgt atggttcact tatcatttgt aataaattcg ttaatgtctc
    22501 cgagtatggt gcttgaatat agataaaatc aaagcgccct tctgcttcaa caatcgcttc
    22561 aatagcctct acataaccac tatcaaattc aaacaatcca atatcgaagt aatcccaact
    22621 cacacctttt ttgtgttgga aaataggttc taaatcgtcc cctccaattt gcaaaactct
    22681 aaatttacgt ggcatcattt tcaccttcta ttaactcatc gagctgatta ataatattct
    22741 tagaagcata tgcatctatt aattttaaag aataggcgta cgcataattc caatttttca
    22801 aataaaataa ataataattt aacgcatcat ctaattcatc aactgtattt agaatacggc
    22861 cattgtcata atcagagacg taatctgttt gttggccatt aatttgtgga atcccagcgc
    22921 taattgcact aatttgtaaa tacaaatcag gctcttttga catatctatc acaagtcgca
    22981 acgtccgcaa tgcttctaca acatcatgtt cagcatgtat cgtcttaaca gcaatgatgt
    23041 catcttgatc ttcaggtgtc attaatgctg aaacattaac atccgcattc tgtttaactt
    23101 ggtattcctc atttaccgac gtaatacatt cacgaagcca catcggtatg tcattttgat
    23161 ggcgcgataa taaaattaaa cggtaataat cttcatgtgc gatataatcc acaagtcgtt
    23221 gcatcatttg ttgcaaatca gcgtcactca taccatctat ccatacacct ataaacgttt
    23281 ccatcaattg actacttata ttaggtgatt gtctcgtttc aaatggtgtg attcgaatca
    23341 ttgtattctc cctctgatac ttctcttgat gactaattaa atcacgttct agttgcacac
    23401 tatcaacaat taaatgggca ttctttacga tagattgata ttcctcatct gacacagttt
    23461 catttctatt tttaaaaaat gaataactta ataatttcgc cggaatatga ttggctattt
    23521 gtcgattatg cctagcatct gaagccacaa tcacatgatc atcttcatgt atttgttgtg
    23581 caatcattgc ttgaaatttt tcttcaatta gttgagccat attgttatat tctgtttgtt
    23641 gatagtgatg ttgatatctt tttgaaacag tgactctgcc atttttcaaa tcttcatgaa
    23701 gtacacaatc tccattaatc gttaaatatt cttggtaaga agcctctccc tgattatcaa
    23761 aataacgtat cgctgataaa taacctctgt catcaaaaat ataacgccgt tgtaactgat
    23821 ctctttcaaa ttcttcaaac caaattgaat acccttcttg actaaaataa atatttgtat
    23881 aggtctgttc actcgtcaca cattttaata aatacggcgt gtacacaaac tcaacatcat
    23941 ccggccattt taagtgatga taattaatcg cttgtggcgc atggtgactg aatccttgaa
    24001 tttcatcaaa cacagaccaa tactttgttt catacaaatc atatcgatgt aaaaatgttc
    24061 ttatattcgg cgcatgattg agaacaatca gttgataatc taaattattt tcaaggtgca
    24121 ttcccattaa actaatcata tcgtcaaatt ccgtcttatt ttgcgtctga taatacggca
    24181 cagtcgtgtc ttgccaccat cgttggtcat cataccaagc tggaataaag tatttcataa
    24241 ttacctcctt accaatactg gtttaaaaat ggcttatatt tatcaaaata taaatatgta
    24301 cgaattgttt ctgcaatatt aatactgatg taaactaata caatcagttg tactgagaaa
    24361 taaatttcag tagataaatg cggtacaaac aatgtgaaat aaagcggtat accaataatg
    24421 attgtaacta atgccgatcc aaaccaacat acgcgtcgtg cttgataatt taaataacgt
    24481 tctgtatcct taccaggttt aacttctgaa aaataattac cacttttcaa gaagtctttt
    24541 gatttttgtt tagtattgat taaaaatctc gataaaaaat aacccaataa catttgaatc
    24601 actaaatata ctgaaatacc tactggacta tcaaacgtca gcattggcat gtcatctgat
    24661 atgcttttat taaaaattga taaaataaaa tgaatgccgc tttttaagaa aacaaaagct
    24721 gaaatgctca tcattaatgt aatactgcct gcaggattaa ccttccaaga caaataagat
    24781 ttcatatttg tagccgagac attcatcaaa tcaatatacg gaattctaac ttctactaat
    24841 tcaatgaata ataggataaa caatgtgatt atcacaagaa tgattaacaa cgcaatcaca
    24901 atatgacttg catctatata ttccattttt tgatgcatca ttgatttaat aatgctaacc
    24961 attacaatcg gcattggtcc tgcgataccg taacgactat ttttgtcagc taaccaaact
    25021 aataacatcg tcccagtaac caaaatcaat attgttaagt aaatattgtc ttgatgaaca
    25081 cgttgtttcg aaacatattc atgaatcaca aaataacttt gaataacact taaaattaat
    25141 gttaaaatgc gctctttata atgcttctca agacttgttt gtttcatgta tttatccata
    25201 ttccgataag aaatcagcat caagataatc attgatgtta gccacggacc taatcccaaa
    25261 gtaaaaaggt ttaaagtatt aacgtctcca cccatattag aaatagctat tttaaaaaac
    25321 gtctcatgtt ttacttgcat atcgttatac gaaacgatag aaatatttgt acctaatata
    25381 taaataaaca agataaaaca tgtgtaaagt atacgtttat atataatttt atattcgtat
    25441 tgttgtaaaa gttttaacat gttacacctc ttttatatca aaaacattaa aaagactaag
    25501 gatatttcac taattaataa aatctaatat agatttttta gtgaacaacg ccccagtctt
    25561 tttaatttta gccagctata aattcaattt atgcttgaga atcatcttga tcattttcat
    25621 atttcttttt ctttctcttc attaaaccta aaccaactaa taatgtcata acgccaccta
    25681 gtaatccatt ttgttttatt gagtcacctg tatctggcaa tctttcttca ctttgcgcag
    25741 gttcagcatc atgttttgat gatacagatg tcgcacttaa tgtagactga gattcatttg
    25801 tatttggtgc tatggactca cttgttgaaa tagatgtgct cgtgctgtgt gtatgatgca
    25861 tactcattga gtctgacgga tgcattgagt tagattcaga tgtacttgtt gagccggaca
    25921 tacttgttga tgttgagtca gaaatgcttt gtgaaccaga catagatgta ctcagcgatt
    25981 cggatgtgct tgtcgaatcg gatgtgctca atgacgttga tgtgcttatt gacactgatt
    26041 ctgagtcgct aattgatgtt gaatcggatt tgtcttgtga cattgaaaca cttgttgaat
    26101 cggattcact cattgatgtt gagtcagata cgctcgttga acctgaacca gacgtactta
    26161 atgattcgga tacgcttgtt gaagttgaac cacttgttga gtccgatgtg cttgtcgatg
    26221 tcgagtctga gccagatgta ctcaatgatt ctgagtcact gatagaagtt gaatcacttg
    26281 tagattctga ttctacagca ctttgtgaac cactgatact tattgaagtg gaatcactga
    26341 tactgtctga tgttgataat gacgtcgaca ccgacgtgct ttgtgatgtc gatgtacttg
    26401 cacttgctga cattgatgat gatatcgatg tgcttaacga acctgatgcg cttgtgcttg
    26461 ttgactggct ttgtgccata gaatcactta gcgactctga tgtactcgtt gagctcgagt
    26521 cacttacact tattgaacct gatattgagt cacttaaact tgtcgatgtt gaaactgata
    26581 cgcttccgct cattgaatca gatgttgaaa gtgatgtgct cgttgaattt gatccactga
    26641 tgctagacga atcgcttgta gacattgagt cactttctga tgcactgatg ctcatagagt
    26701 caaattgact attacttgtt gagcttgact gcgaatcgct cacacttgtt gacgttgatt
    26761 ctgatccact catactttgc gagctactca atgattttga atcacttaat gaatcagaag
    26821 tgctaagact tgtggaacca cttaaagata ttgatccact taatgagttg gagtcacttg
    26881 tactagtaga atcactcatt gatattgaat cacttagcga gctagactcg cttacacttt
    26941 ctgaaccact taatgatgtc gaagtgctca atgaacctga tgtacttgtt gaagtcgaac
    27001 cacttgttga ttttgaatca cttaatgaat cagattcact cacgctttct gaacttcttt
    27061 gtgacgtcga tacacttaat gatgacgaat cgcttgtgct tactgaatca ctcatcgatt
    27121 gtgagccact caatgaacta gactcgctta cactttctga ttttcttaat gacgttgaga
    27181 cgcttaatga gccagaatca cttacgcttg ttgaaccact catcgatttt gagtcacttt
    27241 cggaattaga ttcacttaca ctttctgaat catttacaga ttctgacata ctttgtgaat
    27301 cagatatgct tgcgctcatt acttcactag ccgatgttga tgtgcttgtc gaatcactta
    27361 acgatataga cgcactcatc gaaccagatg tactcgtact tgttgagtct gatgttgaat
    27421 cactcacact atcagataat gacgttgaat cactcacact tgttgatgta cttgttgaaa
    27481 gcgacatact ttgtgaatca agcgtacttg tacgcatcga agtactagtt gaagctgatg
    27541 tactacgaga gtcacttgtt gatgttgatg tacttgccga tccagatgca cttgtacttc
    27601 ttgatgtgct ttgtgaatcg cttaatgatg ttgatgttga atcggattca cttgtacttt
    27661 ctgatgttga gccagattct gatgtacttt ccgatactga ctcacttgca atagtcgaca
    27721 ttcggattga tgttgatgtg ctttgtgaat tacttaatga tgctgatgtg ctttgtgaat
    27781 cggattcgct cgtgcttata cttgttgagg ttgaggcact ttggcttgct gagtttgagt
    27841 ctacgccgct ttgattcatt gatgcactta gtgacaatga tgtactcgtt gagtcagaca
    27901 aactttctga tgttgacgta cttgtacttc ctgatgttga ttgggacatg ctcaggctca
    27961 ttgatgttga atcggattta ctttcacttg ctgacgttga gccggattca ctctcactcg
    28021 ttgaaccgct taatgatgtc gatgtactta atgactctga ttttcttgta ctgattgaat
    28081 cacttgtcga cactgatgca cttagtgaat tagacttgct accacttgct gaatcactta
    28141 atgatgttga caaacttgta gagtcagaca aacttgtact cgttgacgta ctcagtgatg
    28201 ttgaaacact ctcgctctta aacattgacg ttgattcact gttacttgtc gatgttgaaa
    28261 tggacgcact accacttgtt gaattgctta atgatgttga tgtgctacca gattctgatg
    28321 tactgtctga taaagacgta ctatcgcttg ttgagccact taatgactct gatgttgatt
    28381 cggatgtgct ttcactcaat gattcactta aaaaagcaga tgcacttttc gactctgaat
    28441 cacttgtact atttgattca cttaatgata tagacgtact ctctgaacca gatatcgctt
    28501 cactgataga atcgctcgtc gacgttgagt cgctcgttga atcagactct gatgtactta
    28561 agcttgtgga atcactcata gatgttgatg tacgttctga attacttgta cttagcgatg
    28621 tcgaagtact catcgaacca gatgtgctcg tagaagcact ttgtgatatt gattcacttg
    28681 atgcagttga agctgatttg ctatcactcg ttgaatcact aaacgacgtt gatatgcttg
    28741 ttgaatcgga ttgacttgtg ctcattgaac cagatgtact ttgtgattcc gacgtacgta
    28801 ctgaagcact tgtcgacgtt gatgtacttg ttgaacctga ttgactagta cttagtgata
    28861 atgacttact atcagaatca gatgtacttt gtgaatcact taatgattct gatgtactac
    28921 ctgactgaga cgtgctcatt gaactactta cagacattga tttactgtct gatgcagata
    28981 atgacccact tgtactgata gagtcactta ctatctctga agtactcatc gagtctgatg
    29041 tacttgttga gacactttgt gatgttgcta tgcttagtga tccagaaaca gaaccacttg
    29101 tgctcgtcga atcgcttaat gattctgatg tactcatcga tttagaatcg cttgtactta
    29161 atgatattga tgtactttgt gaatctgatt tgcttgctga cgcactttga gagttggcta
    29221 tgctatttga aatactgata gagtccgagg tgctagctga ctcgctcaat gattctgatg
    29281 tactaattga atttgatgta ctatcactta ccgattgtga tgtactagcc gaatcagatg
    29341 tacttgttga taagctttgt gacttagaca agcttcctga tgtactcatg cttactgaat
    29401 cacttaacga tgttgatgtg cgcaatgaat cagatgtgct tgttgataga ctttcggatt
    29461 tttcagtact gctagagttc gaaatagaat cgcttaatga tgttgattta ctaactgaac
    29521 ctgacatgct tgatgataca ctttgtgaat tcactaaact tgtgcttgtt gagcttgata
    29581 cactattact ttcagatgtg cttaatgact tagatgcact cacagaatca gataaacttg
    29641 tacttgttga tttcgaagta ctaactgatg ttgaagtcat tattgaacca gatgtgctcg
    29701 tagaagcact ttgtgagtca gctttacttg ttgacacact ttgtgattgt tgggtacttc
    29761 ccgaagttga tacggaatca ctcatactat ttcttgttac ttcatattta aaagttgtcg
    29821 tcgttttgtt accgctcgca tctgtagaaa cgattgttat agtacttgta ccaatgtttg
    29881 ttggtgtacc actaatagta ttatttgtgc tatcgaatgt taatccagac ggcaatcctg
    29941 tgactgtatt tgttactgca ttcccactat tatcttgcgt agcaatttta atcggggata
    30001 ttggtgaata cacttctgat gattgatctc ctattggtgt cactgttggt gctgtcgtat
    30061 ccacaacatt tattgtaaaa gttgtcgtcg atttgttatt tgcttggtca gtagatacta
    30121 ctgtcactgt tgattgacca atgtttgttg gtgtcccaat gattgaattc gttgcactat
    30181 cataacttaa tccgcttggt aatcctgtaa ctgtatttgt cacagtccca gtaccattat
    30241 ccgttgtagt cagcactata ggattcattg ttttacccac ttcaatggtt tgattgccta
    30301 cagttacagt tggtgctttt acatcagtaa aataatatgt cactgattgt ccagcattcg
    30361 tcatttttac agttttattt gtatcattat aagttgacgc atatgaacta tcgacggacg
    30421 tgtagttata tcctttagca gtcaatgcag attgctgatt atcgattgtc acgacttgat
    30481 caacatttcc agaatatgtt tttgggggaa taatatcttt acctgttgtt acatcaacgt
    30541 atctcacttg tgtaacagca gattctgtat attcgaatgt tccaaattgt acttgttgta
    30601 aatttgtcgc gccacctgtt gaggctgtca ttgataatga aaagttggtc gtaccacttt
    30661 ttgcaatcca atctgaaata ttacgtgtcc atgtttgacc tgcatattta acggtcataa
    30721 cttttgtatc accattataa ttaatatcaa aatcttggaa cgtgttattt gttggttgaa
    30781 catttaactt cgcagcgtta tcagctgttg aatttgatgt gtacgttgtc gcaacgccat
    30841 aactatctgt tgtaacaaag gcaccaaacg caccaccacc agctacatta gatgggtctg
    30901 cattcgcttt tgcagctgaa tttggtttag atgtattgtg atacgtatcc aatttgaagc
    30961 caaatgcgtt acttaagcca ccaataccta ctgcggcacc gtttaaccct gtttcaccta
    31021 atacacctgg cgaaaaggca aagccgatac catctccacc atttccatta ccttcgtatt
    31081 tgttacctaa atttactttt ccagaaaaat gaaaactctt attagagtca atacgtgttc
    31141 ctaatgtaat ggcacctttt tggctgtatg catcctgtgt taacgtcaca ataccggtac
    31201 tttgatcata ggtagcatta cctgacgttg tcatatattg tttgaggttg tctttattaa
    31261 ccgtaatcgt attagcggtt actgcacttg tcgttgctgc tgacgcaaat gttgacatag
    31321 ctaagcgact gaaagttcga agttttactg gtgcggtgct agttgacgtt gtgctagttt
    31381 tgtttaagtt ggctgaagat ggcgttgtgc tttgtgaagt gttatttgat gcagtacttt
    31441 gatttgttga tgtattaatt ggttgttctg tacttgaagt tgaagctaca gatttagtat
    31501 cagaacttga tgtagtattc tttgaggatg ttgattctga tgtagatgtt aatttttctt
    31561 gttgattgct tgtactatta gttgtcgaag tgacattttc agacttttca cttgagacta
    31621 tgtcactatt tgatgtttgt accgaactac tatttttcgt tacgcttgtg gaatcggctg
    31681 ttgatgttga tgcttcgatt gtcgttgagt tttgattacc tactgtttca ctttgtgtat
    31741 ttaattcaga tgttaatggt gcatcagaag ccgcaaaagc ttgctggtca tgtagcatat
    31801 taacagtaaa tgcaccgcca ataactgccg tcgttttcag tccgtatccc gtcatttttt
    31861 tactaatgct ttgattatct tgactcacta tactatgact aataaatggt agtcccatta
    31921 ttttgaacat ttctatttct ttaattccgg atttcaccca attttttcca gatttataaa
    31981 gtcttactct tgttttttcg tttgctaagc tgtcatgaaa ttctttctgt cttttactca
    32041 tgtaataact ccttgtatta tctttacatt cattagatta taatatatgc cactattcaa
    32101 tttaatacaa ctcttttttc aaataaaaaa atacttaatt tgtcaaaatt tgtaaaaatt
    32161 cttttttatt cgtctatatg gaatcgtttt catattttta aaattacttt ttctcgttta
    32221 tgcgtataat ctttttttat ataaatttgg ctaattggct ttatgtttaa tcattataat
    32281 tgtttcgttt ttaaaataat tattgtatta atatatctat atcatccacc ttttatttat
    32341 aaatagttaa ttt
//
LOCUS       DAHHRR010000021        30225 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.021, whole genome shotgun sequence.
ACCESSION   DAHHRR010000021 DAHHRR010000000
VERSION     DAHHRR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 30225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30225
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.021"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            44..1681
                     /locus_tag="NBI42_000773"
     CDS             44..1681
                     /locus_tag="NBI42_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCD1777212.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMKHLMENGSISTYGLLAVYLIIIIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WKGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWATLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            2171..3523
                     /locus_tag="NBI42_000774"
     CDS             2171..3523
                     /locus_tag="NBI42_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HCD1777213.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESAHNKAQHFQKMMLDYLDTDDVTSLSTDDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     HTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(3585..4772)
                     /locus_tag="NBI42_000775"
     CDS             complement(3585..4772)
                     /locus_tag="NBI42_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1777214.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(5166..5942)
                     /gene="fetB"
                     /locus_tag="NBI42_000776"
     CDS             complement(5166..5942)
                     /gene="fetB"
                     /locus_tag="NBI42_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCD1777215.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLVLCVFVIIVNASWNTISRSSPVMHHVFLISFVAIFVGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            complement(5935..6597)
                     /locus_tag="NBI42_000777"
     CDS             complement(5935..6597)
                     /locus_tag="NBI42_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1777216.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLISPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDKKRAKQLIKDVGLGHYQLSSKIEHMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDINNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            complement(6845..7921)
                     /locus_tag="NBI42_000778"
     CDS             complement(6845..7921)
                     /locus_tag="NBI42_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD1777217.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            complement(8276..8542)
                     /locus_tag="NBI42_000779"
     CDS             complement(8276..8542)
                     /locus_tag="NBI42_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCD1777218.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            complement(8542..8799)
                     /locus_tag="NBI42_000780"
     CDS             complement(8542..8799)
                     /locus_tag="NBI42_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD1777219.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            9158..9613
                     /locus_tag="NBI42_000781"
     CDS             9158..9613
                     /locus_tag="NBI42_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCD1777220.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKNLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(9781..11358)
                     /locus_tag="NBI42_000782"
     CDS             complement(9781..11358)
                     /locus_tag="NBI42_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HCD1777221.1"
                     /translation="MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            11553..12302
                     /locus_tag="NBI42_000783"
     CDS             11553..12302
                     /locus_tag="NBI42_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777222.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIVIVF
                     STLGLIAIFTQQAPWDFFFPISLNISCLLAVIISLAIFRRMHEDAFVPMIRSQIVASA
                     KYKWHILISFTLSIVLFLLLNHFDIFALNVDAFTVFDIYPLIREYGSISLIAIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSVILETLYYYPYINTWWEFIPALILAS
                     SATYLYLKSNRNFMVSYFYQLAVILVLHIFM"
     gene            complement(12524..13717)
                     /locus_tag="NBI42_000784"
     CDS             complement(12524..13717)
                     /locus_tag="NBI42_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1777223.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNASNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGVSLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSLLKKPTSR
                     PANSL"
     gene            complement(13729..14478)
                     /locus_tag="NBI42_000785"
     CDS             complement(13729..14478)
                     /locus_tag="NBI42_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1777224.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKDVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(14471..15286)
                     /locus_tag="NBI42_000786"
     CDS             complement(14471..15286)
                     /locus_tag="NBI42_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1777225.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVRDVHFTVTKGETLGVIGESGSGK
                     SITCKSIVGLNPERLRVTGDITFDGKPMLSLSEAQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEHGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(15286..16152)
                     /locus_tag="NBI42_000787"
     CDS             complement(15286..16152)
                     /locus_tag="NBI42_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1777226.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVILGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDSLQVAIDPRISSK
                     EKLRSVKKGVVQ"
     gene            complement(16149..17084)
                     /locus_tag="NBI42_000788"
     CDS             complement(16149..17084)
                     /locus_tag="NBI42_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1777227.1"
                     /translation="MFKFILKRIALMFPLVIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(17097..18695)
                     /gene="cntA"
                     /locus_tag="NBI42_000789"
     CDS             complement(17097..18695)
                     /gene="cntA"
                     /locus_tag="NBI42_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HCD1777228.1"
                     /translation="MRKLTKMSAMLLASGIILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDISEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQQNKKLHSWLKISTLIDNVKVKDKYTVELSLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLDKVSFTQSQYELPFNEMQYK"
     gene            complement(18835..20139)
                     /gene="cntM"
                     /locus_tag="NBI42_000790"
     CDS             complement(18835..20139)
                     /gene="cntM"
                     /locus_tag="NBI42_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HCD1777229.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     RAYKKEKRFEVKVQNEAHRHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFSQDIEVISFSTYLGDTRILDKEVPN
                     HVLTTGVKKKLYMGSTHSNSTMCQHINALSEQLNIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDEKGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     RCQAYKDMHQDQQLSSQFNTNLFEEDKALVTKFLEINRTLSY"
     gene            complement(20132..20950)
                     /gene="cntL"
                     /locus_tag="NBI42_000791"
     CDS             complement(20132..20950)
                     /gene="cntL"
                     /locus_tag="NBI42_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HCD1777230.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASHDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKALFNYPSQETTKDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(20961..21782)
                     /gene="cntK"
                     /locus_tag="NBI42_000792"
     CDS             complement(20961..21782)
                     /gene="cntK"
                     /locus_tag="NBI42_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HCD1777231.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNNFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVNQVTTE
                     IQHLVETFVREQQWNDKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(22582..22947)
                     /locus_tag="NBI42_000793"
     CDS             complement(22582..22947)
                     /locus_tag="NBI42_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777232.1"
                     /translation="MLNEKESFRLLYQAIRELADKIGDNQLETNSISLLLLDFDFEHE
                     VFDKLYLAILKYLNTVSIENINHIDLLNLIDNTIPEDREINTFVKNKIIIGFANNYFP
                     ELQILANEIKSDMASSLKQ"
     gene            complement(23211..24029)
                     /locus_tag="NBI42_000794"
     CDS             complement(23211..24029)
                     /locus_tag="NBI42_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1777233.1"
                     /translation="MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDISEAVSE
                     TVNKIESNGDNAKAYIVDIASEQQIDNFASEIREQIGHVDVLFNNAGVDNAAGRIHEY
                     PTDVYDKIMNVDMRGTFLMTKMMLPLMMTKGGSIVNTSSFSGQAADLYRSGYNAAKGA
                     VINFTKSIAIEYGRDGIRANAIAPGTIETPLVDKLIGTSEDEEGKAFRENQKWMTPLG
                     RLGKPEEVGKLVVFLASDESSFITGETIRIDGGVMAYTWPGEMLSDDSWKGTLE"
     gene            24265..25803
                     /locus_tag="NBI42_000795"
     CDS             24265..25803
                     /locus_tag="NBI42_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCD1777234.1"
                     /translation="MTSKERQKGSIVNSFLNSVEKIGNKLPDPSVLFFLMCVGLAILT
                     WLVSLFNVSVKHPGTHQTIHVKNILSHDGFTMIMNDAIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTDPATRIVSESIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGQYDDRLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPSGP
                     IFVK"
     gene            complement(25915..26337)
                     /locus_tag="NBI42_000796"
     CDS             complement(25915..26337)
                     /locus_tag="NBI42_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCD1777235.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDKL"
     gene            complement(26789..27208)
                     /locus_tag="NBI42_000797"
     CDS             complement(26789..27208)
                     /locus_tag="NBI42_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1777236.1"
                     /translation="MSKKKILILTSIMLIILISVAGIHFKMKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYINENKEDDFVAYGSPEHNYQ
                     FGGDMIESEKLSELLKPANQLKSPDEIKEELDKKEGH"
     gene            complement(27680..28102)
                     /locus_tag="NBI42_000798"
     CDS             complement(27680..28102)
                     /locus_tag="NBI42_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1777237.1"
                     /translation="MTKKHVFIIIGVILCICIVATVIYLKVKHDEKEKQKALYYKEQQ
                     ERITLYLKHNTKEPNTIKTVHFTILKTGPMGDAVIEGYINENKKADFVAYGSPEHHYQ
                     FGGSLIKSKNLSTLLKPAHQTKTPDEIKKELESKKNDR"
     gene            complement(28354..29049)
                     /locus_tag="NBI42_000799"
     CDS             complement(28354..29049)
                     /locus_tag="NBI42_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1777238.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGVKVILAGRNKDKLQ
                     NVANQLAQDSVKVVPTDVTKKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITATYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
     gene            29349..29630
                     /locus_tag="NBI42_000800"
     CDS             29349..29630
                     /locus_tag="NBI42_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1777239.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEIKKLDDVRMPFSSAHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            complement(29890..30081)
                     /locus_tag="NBI42_000801"
     CDS             complement(29890..30081)
                     /locus_tag="NBI42_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777240.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
BASE COUNT         9645 a         5683 c         4510 g        10387 t
ORIGIN      
        1 gcatattatt ttttgtattt ttggcaataa cgaaggagta tttatgaata aagacaagca
       61 attgcacaac gacaaaatca atctatccca attagtctta ttagggttag gttctttaat
      121 aggatccggt tggctatttg gtgcgtggga agcatcatca atagctggac cagcagcaat
      181 catatcatgg gttcttggat tcctagtcat tggaaccatt gcctataact acattgaaat
      241 cggcacaatg tttcctcaat caggtggtat gagtaactat gcccagtata cacatggctc
      301 attattaggc tttattgctg cttgggcgaa ttgggtgtct ttggtgacaa taatacctat
      361 cgaagctgtg tcagctgttc aatatatgag ttcttggccg tggcattggg cgaaaccaat
      421 gaaacattta atggaaaatg gctctattag cacatacgga ttgctagctg tatatctcat
      481 cattattatt ttttcattat taaactattg gtccgtaaaa cttttaacat catttacgag
      541 tttaatttct gtatttaaat taggcgtacc catgttaacc atcatcatgt tgatgttatc
      601 aggattcgac acttcaaatt acggccattc ggcaagcaca tttatgcctt acggaagtgc
      661 accgattttt gctgcaacaa cagcatcagg gattattttt tcattcaatt ctttccagac
      721 aattattaat atgggttcag aaattaaaaa tcccgaaaaa aatatcgcaa gaggcatcgc
      781 tatctcactg tcaatcagtg cagtgttgta catcatttta caaagtacgt ttatcacttc
      841 tatgcctcaa tcaatgttac aacatagtgg atggaaaggc atcaacttca attcaccatt
      901 tgctgattta gctatcttat taggaattaa ttggctcgca attttactat atattgaagc
      961 ttttgtatca ccattcggta ctggcgtgtc atttgtcgcc gttacaggtc gagttttacg
     1021 agcaatggag aaaaatggac atatccctaa atttcttggg aagatgaatg aaaaatatca
     1081 tattccacgt gtagcaatca tctttaatgc catcattagt atgattatgg ttacgttatt
     1141 tagagattgg gctacgctag cagcagttat ttctactgca actttagtag cctatttaac
     1201 tggcccaacg acagtgattg cattaagaaa aatgggacca acaatgactc gtccatttag
     1261 agcaaaaatt ttaaaagtaa tggcaccatt atcatttgta ttagcttcat tagctatata
     1321 ttgggcaatg tggccaacaa cggctgaagt tattttaatc attatacttg gattaccaat
     1381 ctacttcttc tatgaatatc gtatgaattg gcgtaataca aagaaacaaa ttggtggtag
     1441 cttatggatt attgtatatt taatcgtgct atcaatactg tcatttatag gaagcaaaga
     1501 atttaaaggc ttaaatatga ttcactatcc atttgacttt atcgttatta ttattgtggc
     1561 gcttatcttc tattacatcg gtacaacgag ttcatttgaa agcgtctatt tccgtcgcgc
     1621 tacacgaatc aatacaaaga tgcgcgagtc actaaataat gaatcaaagt catctcatta
     1681 aatattttaa aatgcagtgc catatatgat ttcaacacac acattaacca accattgact
     1741 tcaacatctt ggttggtttt ttattttgaa aatgggttat aaataactaa cataacaaga
     1801 tggcgagcag gctgggacat aaatcaatgt tctatgctct acgatgttat attggcagta
     1861 gttgactgaa cgaaaatgcg cttgtaacaa gcttttttca attctagtca ggggccccaa
     1921 caaagagaat ttcgaaaaga aattctacag gcaatgcaag ttggggtggg acgacaaaat
     1981 aaattttgcg aaaatatcat ttctgtccca ctcccgtaga agaacatcat ataatgctga
     2041 aaatggtttg agttaaaaca gatatcaagc tcgtctgatt cagtcacaaa attgtcttgt
     2101 tatacttgtc acctatcatc tatagaccat ggtatgatta aattggggat gataaaggag
     2161 gttaataaat atgaagatta atactacagg tggtcaaatt catggtatta cacaagatgg
     2221 tttagatatc ttcttaggca ttccttatgc agaaccacca gttcatgaca atcgctttaa
     2281 acattctacg ttaaaaacac aatggtcaga gccaattgat gcaactgaaa tacaacccat
     2341 cccaccgcaa ccagacaaca aattagaaga ttttttctcc tcacaatcta caacttttac
     2401 tgaacatgaa gactgtttat atctaaatat ttggaaacaa cataatgatc agacgaagaa
     2461 acctgtcatc atttattttt atggtggtag ttttgaaaat ggtcatggca cagccgaact
     2521 ctatcaaccg gcacatttag tacaaaataa cgacattatc gttattacat gcaattatcg
     2581 tttaggcgca ctaggatatt tagactggtc atattttaat aaagattttc attccaataa
     2641 cggactttca gatcaaatca atgtcataaa atgggtgcat caatttattg aatccttcgg
     2701 tggcgacgct aataacatta ctttaatggg tcagtctgca ggcagtatga gcattttgac
     2761 tttacttaaa atacctgaca ttgagccata cttccataaa gtcgttctgc taagtggcgc
     2821 actacgatta gacacccttg agagtgcaca caataaagca cagcatttcc aaaaaatgat
     2881 gctcgattat ttagatacag atgatgttac atcattatcg acagatgata ttctcatgtt
     2941 gatggcgaag ctaaaacaat ctcgaggacc ttctaaaggg cttgatttaa tatatgcgcc
     3001 tattaaaaca gattatatac aaaataatta tccaacaacg aaaccaattt ttgcatgtta
     3061 tacaaaagat gaaggcgata tttatattac tagtgaacag aaaaaattat cgccgcaacg
     3121 ctttatcgac attatggaat taaatgatat tcctttaaaa tacgaagatg ttcatacggc
     3181 gaagcaacaa tctttagcga ttacacattg ttatttcaaa cagccgatga agcaattttt
     3241 acaacaactc aatatacaag attcaaacgc acaactatgg cttgctgaat ttgcatggca
     3301 tgatacttct agcgcacact atcgcagtgc ttaccatatt ctagatatgg tattttggtt
     3361 cggaaactta caaatattag cagcacatca atatccaaca actgctcacc ttaaattttt
     3421 aagtcgacaa atgcaaaatg atttagctaa ttttgctaaa agcggtaaaa tgccttggcc
     3481 gatgtatcat aatgaacgtc gctattatcg cacatatcaa taacgttatc gcatctaaaa
     3541 aagcacacct gaatttgata gcaggcgtgc ttttcatatg agaattatga tgatttaaat
     3601 gttttagcat aatgattatt tttcaatgtg aaaacaacga ttaatcctaa tgcgccaatg
     3661 atggcactcg tatacgtgat agcttgaaca gaaacatgtg tcatgaccaa gcctccaata
     3721 atgccaccaa caccaatacc agcgtttaaa ctagacatgt tccaactcat tacttggctt
     3781 gtgtcgcctt caacatgttg aatcacaccg ctttgcactg ctggattagt actccattgc
     3841 atgatattcc aaataaatag tcctgctaac aatagacctg aaccaggtaa gattaaattc
     3901 ataagtaaca tcatgacgat aaaaatagaa acagaaatca ttaaccaacg cttacttgta
     3961 attttatcgg agaatatacc acctaatgat gttccaataa cgccagcgat tccatttact
     4021 agaagtgcta atgaaacgaa tgacatatca tgaccattag ataaaataag tggatttata
     4081 aagacgaatg tcactgagtt tgcaatcaat actaaaaacg taataattaa atattttgct
     4141 acttcagcag gtcttaatat tttcgaagta acatgatttt catgagatgg tgcttcatga
     4201 ttcacagggc ctcgttgtat ttcctgatcc ttcggtaaat agatcatcat caagaagcca
     4261 acaataatac tcacaataat taagaataga aatgtatagc gccaacctac taaatcgccg
     4321 ataaccgtgc caattggtac accaaaaaca ttggcaccac taaaccctgt atagacaagt
     4381 ccaatcattt tgccacgatt tttaggtgct gataacatcg ctgtaatagc taatactttt
     4441 acgataatta gtgctgctgc tgcagatgag ataattctac caactactaa tattgaaaaa
     4501 tttggcgcta cagcaataat gccattacca atgataaata taagtaatgt ccataataat
     4561 acaggccttg atgaaaaacg gttcgttaat ttaaccagaa taggtccaca tatcgcaaat
     4621 gttaaagcgt acattgtcac taattgacca acgacagctt ctgatacatg taagtcctga
     4681 ctcatcaagt tcataattcc tgcaaccatc atttcaacca ttcctacgat aaaaatactt
     4741 aaaatgaatg taaatagtcg catgattgac attttaaaac tcctttcaaa caaaatttcc
     4801 gatattcatt tcccttagaa taacgattta ttaaaaaatg tgtgcaacac aattttatat
     4861 ataaccgttc tattatataa tattttgctt taattttctg tgcttaattg gaaaattatt
     4921 gaaattgttt ttcagtattt gaaaaattaa cacaagtatg tcatttaata attatgtata
     4981 attacttcag tttcaacaat gactccaaaa aagcacgcac gctttatatg tcagaacaga
     5041 taactgacta caaagtatgc gtgcctttat gaatattttc gctgcgctaa ataatagcat
     5101 tactatatgt gcatcacttc atttccaatt aaaactaatg cctaaatctg atgcggtaaa
     5161 atctatcatg actcactctt aacatccgta tttcgtgcta ctaattgatg tcttgcattg
     5221 aaaaattgac catagcttaa atatgtcgca atcaaagcag acataatggt cgcagttgta
     5281 tgaataaaca cgactaacaa ttgaaattta atcgcttgta aaggtggtac gccaccaata
     5341 attaagcctg tcatcatacc aggaatcgac acaagcccat atgttttaac cgaatcaatt
     5401 gttggcacta tagctaaacg aatactttca cgtattgcac ctttagaagc caatttaggt
     5461 gtagctgcaa gtgataattt agattcaata ttagtaccat cttgtacgaa tgcacgatct
     5521 aaattctggt aagctaaatt aattgcaatc aagccattat ttgcaagcat gccgccgata
     5581 gggataactt cattggctgt aaattggatg gcccctgtcg caatagtacc tgcaagcggt
     5641 aatgccgttc caacaaagat agccacaaat gaaatcaaaa agacatggtg catgactggt
     5701 gatgatcgac taatcgtatt ccatgatgcg ttgacaataa tgacaaatac acataatact
     5761 aatagccatt tgtcattaat tttaaaaata tagtgcagta agaatcctaa aatgattaat
     5821 tgtacgaccg cacgtagtgt cgccacaatc aaatctttaa taatatgtag accttcttta
     5881 taagaaataa taattggaat aactaatagc aatgccgtca gtcctaatgc tgtattactc
     5941 atgttggttc aactcctctg tattagaaat ttgaccatca acaattgtta tacgcttttg
     6001 aaagtgtcgc atactttggt catcgctgtg ggtaatccac ataattgcca cgccttgatc
     6061 tgctaattta aaaatgatat tttctatttt ttctttatta ttaatatcta acgcacttgt
     6121 tgattcatct aatagaagga tgtctggggt atacattaat tggcgtgcaa tcgcgatcct
     6181 ttgacgctcg ccacctgaca tatgttctat tttagaactt agctgatagt gtcctaaacc
     6241 tacatcttta attaattgct tagcacgttt cttatcaaat ttatcattac gcgcaagaga
     6301 cggaaaaatc atattatctt caatcgtttc accaaacaaa tcgctttgtt gcatcaaata
     6361 actgattcgt tgacgcaatt cttccgggtc ataatcatta tagggtttac ctttaaaata
     6421 aagttctcca ctagttggac taatcaaatt acatatttgc ttttgaaatg tacttttacc
     6481 actacctgat ggacctataa tggcaatact ctcgccttta tctactttta gactgatatg
     6541 atctagtatg attctatcgc tcgctttata cactaaatct ttaatttcta acaacatttg
     6601 caccgctcct catgtaatat atcaggtata ttattcccgt ttttttgatt tttagtgcaa
     6661 ttacatttaa gaaaaaatca ttttcttgta atatcaaaat ttaggcataa ttgattttca
     6721 gcatttaaat taaaaacaaa aaatcctatt aacatggata ctgtctaatg actaagaatt
     6781 aaagttagac aattgtttaa caacctacca tattaaaagg attctttatt atttgtggat
     6841 tgggttacca catgatattt ttataacttt catcatttaa tgaacggaca atttcagtaa
     6901 caagacggat agaattttca taatcatcta cattgagcac tgaaacatta gaatgcatgt
     6961 atcgcagcgt aacacctatt gtcatcgttg gaataccttc atttgcgaca tgaatacttc
     7021 ccgcatctgt acctccacct ggtgtcgtat cccattgtac ttcgatgtta tgttccttag
     7081 ctacatcttt aatatgctta cgcaaacctt ggtgagcaat acttgtagca tccatcataa
     7141 tgacaactgg accaccgcct agtttactat cgctcgtttg acctgacata cctggggtat
     7201 cataagcaat acctacatcg acagctatcg ccaagtctgg tttaatcgta ttcgctgcca
     7261 ctttcgcacc acgcaaacca acttcttctt gcactgtggc accactgtat aagttaatgc
     7321 caatattttc atcttttaaa cgttttaata cctcaatagc taatgcacag ccatagcgat
     7381 tatcaaatgc tttcgcagtt aaatatttat catttgcaag cacttcaaat tcactatatg
     7441 gcgtaaccat attgcctact tcaacgccag cttcttcagc ttcttccttg ctactaacac
     7501 caatatctat aaacatattt ttgatttcca ttggcttttt acgttcttca ggcgttaaga
     7561 catgtggcgg tttagaaccg atgatacctc taatttcttt gcccgaatct gttgtaatcg
     7621 ttactttttg agatagcatg acttgattcc accatccacc aactggcgta aatgaaataa
     7681 aaccatgttt atcaatcttt gttaccataa aaccaacttc atccatatga ccagaaatca
     7741 taattgagta ttgaccattc tcagcatttt tctttccaaa aatgccaccc aagttatctt
     7801 caataatttg atcactgaca ggctctatat agttacgcat tgcttcttta acttgcattt
     7861 cataaccagc aatgccgttt acatctgtta aatgtttaag taatttgact gactgattca
     7921 tgttattcat cctcctgtat ttcaacttaa aactattgta tcactaactt tcagaaaaac
     7981 gttccctacc gctttatcat tttcaggctt aaatattttt aaaattttgt gaggaatccg
     8041 cttaaaaacc tcatatttct aaaatttaat attccgaaaa agtcacaatt taaaattcct
     8101 tattataata cgttattctt ttattgttac taatttcaat ctgtttagtc ttgcgctatt
     8161 ataaaattaa aaagtgtaga gataacaacg cattaacgtc gctattttcc ctacacttaa
     8221 atactagttt ctttaattaa cttatcatta aagttattca gatattgaat attcattaat
     8281 cataatgtga ccatgccgat agtattaata cttctttatt tcgatcatct acggtataga
     8341 cgaccctgtg ctgatggtta attcttcttg aatatcgctc taaatattta ggctctaatt
     8401 tttcaaaaga ttgtgttatt ttatacggat cattttttaa agtctcaaca atttctaaaa
     8461 atgacttctt taaataagaa tgttttattt tctttaaatc tgatttcgct gaatttttaa
     8521 tcttaaccgt gtaattgctc attaaagatt atcccaatca atatcatcta tatttgttgt
     8581 accactatta tctttttctc tttctcttac tttgtccatt gtaccagtag attcaagata
     8641 tattgtctct tgtatacttt tccaatcttc taaacctata atcacagcat tattttcggc
     8701 attattgcca ctaatataaa ttggttcgtg attattatta acatttttta gtaattgata
     8761 aaaatctttt ctcgcttctg tagggctagt gataatcata taaaccctcc tttgttttct
     8821 gttacgctaa ttatagcgta cgcacttgag tacgtcaata ttaccaacga gttcatcgta
     8881 ttcttaatac ctatatgcta tcgttcacaa acttttgttc ttttcttcca acataccgcc
     8941 tataatgctc aaaaagaaat cagcagtgga acacactacg ttccctaccg ctttatcatt
     9001 ttcaggctta aatattttta aaattttgtg aggaatccgc ttaaaaaaac tcatatcttc
     9061 aaaaaaaatg acaaaattta aatttttgaa taacatcatt ttttagtaaa tgttataatt
     9121 tctttataat acaaatatcg aaagaaggcg actgactttg aaaaaattat gttcattaat
     9181 tgtagtagca ttagtttgta tcattgcatt atcagcttgt ggtaaagaac aaaccaaaac
     9241 atatgaaggt gatgttagtg gtaaacatgt actaacttct atcacatata aagatgataa
     9301 agttttaaaa cagtctacaa tcaacacaat taaatatgac gatctaggta tggataaaga
     9361 cgaagccaaa aagttattcg ccaaatctga aagtattttc aaaaacctta aaggcgtaaa
     9421 atacaaagta gactataaag ataaaaaagc aattgaacac ttagacatag attacacaga
     9481 agttgacatg aaaaaattaa ataaacgtct tggtgtttcg actaaagaaa ataaagatat
     9541 tagttttgaa aaacttgaaa agcaattaaa gcacagaggt ttaaaagaaa aagataaaat
     9601 ggacgacaaa tagtttataa cttaaaaatg ccctcagata agactaagtt tacaaacctt
     9661 aattcatatt ctgagggctt taatatttga agttcttgtg tgaccagcat ccactactaa
     9721 tataaaatta tttgcagtaa cgctaaaatc cgctgctttc aatttcccga aataattaag
     9781 ttaactaatg agttttaatt tataatcatg tatcgtttgt aactcaccat cgacttttcg
     9841 atatacaata tgatcagcag taatttctgt aggactggat acgccaacag ctgctgcaat
     9901 attgaataag ccttcatgca aacttgttac atagtttgtg acacgatatt gcttttctcc
     9961 aacaatcaat gctttttctt tcttcgcatc tgtcgttgca acacctacag gacatgtatt
    10021 catatggcat tgttgactca taatacaacc gacactaatc atcatgccac gtgcgatatt
    10081 tacaaaatct gcacctaaac ctaatgcaat cgcaatttta tctggtgtca ctaacttacc
    10141 agatgccgcc aatttcactt tatctcgaat accatatttt tctaacatgc cagacacaat
    10201 aggtagagct gtaaatagcg gtaagccaac accatcttgt aattcttgga atgttgcacc
    10261 agtaccacct tcaccaccat caatcgtaat aaagcttgga tacttatcta gttccaccat
    10321 cgtacgtaca agtgtttcaa tttctgaaac tttgcttact acaattttga atcctactgg
    10381 tttttgacct aattgctgca actgatcgac gaaacgaatc aaatcttcag cattatgaat
    10441 aaattcgtaa cggttaggtg aattgattgt tttataaggt tccacatttc ggattttagc
    10501 aatttcttcg tttacctttt cagcttccat atgaccacca cgagtcttag caccttgtgc
    10561 caacttcagc tcaaatgcac gtacgttaga taactgtgcg acctctttaa ataaaccttc
    10621 actaaaatta ccttctttat cacgaacacc aaataaaccg ggaccaattt ggaaaatgat
    10681 atccccatta ccttttaaat gatattctga taagccacct tcacctgtat tcatccaagt
    10741 gcccgcttta gctagacctt tagataaagc tgtaatggca ttttttccta aggctccata
    10801 actcatacca gattgtccta cgatacgttt taaaataaat ggatgtttta aatgttcacc
    10861 taattttatt gcatggtcat cacttaagta atacggatca atctttgtcg gtacacgata
    10921 ttcttcacga ctaaatagac gctcattcgc gattttataa atgaatgttg ataacaatgt
    10981 tgtattatct actgaaatct cattacgttg catcggaaac attgtgttct gtatgtaaaa
    11041 gccgtcttga taatctttag tagtaccgaa gctggtcata cgagagttat attttccagc
    11101 caaaacgata tttttataat cattacgtga aaaaggtttc ccttcattat ccccagaaaa
    11161 taaatactga cgtaattccg gtcccatttt ttctgaaata tatctaatac gtgctagtaa
    11221 aggataattc cttaatacac tatgttgtga ttgtctttta tctttaatta accaaataag
    11281 cccgataaca ataaccgtaa gcatgaatcc tacaacgata atgttaacta taaattgcat
    11341 gactgtaaga aacgtcatta caatacctcc ccccaaaatt tcaattcaat atttatgata
    11401 caccttacaa aacaaaacac aatggaagcg cttcatttta taaaacaatt ttatgatatg
    11461 tttttcattt taaattttaa tgtataaaac atacaataca aagtaatatg tgctaaagta
    11521 tctatataat acaactattt aagaggtata ctatgtcaaa tacaaataaa cattacatag
    11581 aagaagaata cgctactgaa caatcgcgtt ttttcaaacg tgatattgga tttattttcc
    11641 tttatatatt tttggttaac atcttgccga ttgttatcgt gttttcaaca ttaggcttaa
    11701 tcgctatctt cactcaacaa gctccgtggg atttcttttt tcctatttca ttgaatatta
    11761 gctgtttact cgcagttatt atttcactag ctatttttcg tcggatgcat gaagatgcat
    11821 ttgttccaat gatacgttcg caaatcgttg ccagtgctaa atataaatgg catattctta
    11881 tatcatttac attaagtatc gtactctttt tacttttaaa tcatttcgat attttcgcac
    11941 taaatgtaga tgcctttact gtctttgaca tttacccgct cattagagaa tatggttcca
    12001 tcagtttaat agctatattt tcgttaacca ttatattaaa tactttgatt tgccaactta
    12061 ttttccgtca tattttaatt caagaattaa gcagattaat acctttaaag attacaatag
    12121 tattatccgt tatccttgaa acactgtact attatccata cattaataca tggtgggaat
    12181 tcattccagc attgatacta gcaagtagtg cgacttactt atatttaaaa tcaaaccgta
    12241 attttatggt gagctatttc tatcaattag cagttatact cgttttgcat attttcatgt
    12301 aaaatttttc agagtattat ccattatcca tacacatcaa aaagcacact taaaagtcag
    12361 tatcacaact cacttttaag cgtgctttat ttagatatac tgtcgttcta aaaacgatcc
    12421 atatagaatg tttcatgtcc tagcatggca catgaatcgc catgctcaat atacctttta
    12481 aaatttaaat atttattaac ttaatatgct ttaattcact tcattaaaga ctatttgctg
    12541 ggcgtgatgt tggttttttc aataaactca taatcattat tacaatgact aacataccac
    12601 aaattagata catccattta aatcctacta aatcggaaat aggtcccatc aatgcgccac
    12661 ctaacgatac acctaagtct gcacaggcaa taaataaacc taacaacata ttacgaccta
    12721 cttttggtaa gacgaaactt aagtatgatg ttaatgttgg atacaccatt gcttgtgtca
    12781 ttcctattaa tattgcactg ccatagaaaa taattgcacc tacttgcgga ccaaatgcca
    12841 ctacaaatga cgcaatcact aataatgata gtacagatac catatattta ggatgccaca
    12901 taccatctga cggaatgtat ttccttaagt aaaatcttgc cgcaacaact gcgatggcct
    12961 gtattgtcaa aaagattccc gcattcgcga atcctaaact cactgtgtat aacggtacaa
    13021 atgtactaac tgcaccaaat acaatcgatg caacaatcat gataatacca ctgttcaaca
    13081 actctttatt tttgaaaaat tgtgcaaaaa cagttacagc gttaaacggc attttttcaa
    13141 tcttatctga cgtatcgggt tcctgttcag caaaggtcac gcgatagcca aagaatgttg
    13201 ttgttaatgc gataaagata atgacaattg caaatagtga aatattactt gcattccaaa
    13261 tacctacggc aactaatggt ccgattaagt ttggaatcgt tgaaaatagc gagtacaatg
    13321 atacaccttc agaacgatgt tcctctggta atgcatcaat aatacctagc tgtaaagaca
    13381 tagaaaagaa tgccgtacac acaccttgca taacacgtgc tacgaaataa ccttctaagc
    13441 cagtaaaacc ataaataatt aaagctatgg cattgataat caaaataatt cttaatactt
    13501 taatgggacc gacacgagca ataatttgtc ctgcccatgg tcgaaatacc attgctgtta
    13561 acatgtatgc ccccataacg ataccgataa ccgtattcgt tgcgcctaaa tcatgccctc
    13621 gtaaaggtat aaacacgttt aagattgcgt tggcactaaa gaacatcaat gttaatatat
    13681 ataatctcaa aaagggccaa gccattgcac ctttcattat ctcactcctt agaatgatag
    13741 ttgtttttct attaattctc ttgtataagc attgtcactt ttatgcaatg cgcttgtcgg
    13801 aatttgttct tctatttttc cgtttttaaa aataattaat tgatcacata aatacgtggc
    13861 agcttgaata tcatgtgtga taaaaatata actcaactga cgcgtttcac gtaaatgaat
    13921 caataaatct aatatttgtg tttgaattga catgtcgagt gaactaatgg cttcatcaaa
    13981 caaaatatat ttagggttaa tacatattgc acgcgcaatc gcaacacgct gcgcctctcc
    14041 acctgataac atattaggat atttatccat gtatgcctta gatagaccaa cttcttccaa
    14101 caatgtgata gcttgaacct ccattacatc tttaggttgt ccatcacatt gacacatcac
    14161 ttcaaataag atttctctaa cagtctgaaa tgggtgtaat gatgacgtat aatcttgaaa
    14221 tacagcacca atttgatgac gtctcacttt cttcttatgc atcggttgat catttaaggt
    14281 tacacaacct ttatccggtt tctcaatacc taatatcata cgactcaacg tcgatttacc
    14341 gctaccactt tctccgataa tcgcaatcgt cgcaccgatt ggacactcaa atgacacacc
    14401 tttcacgata ggtgttcgac gacgcttaaa aacatgtgcg ctttgatatg acttttcaac
    14461 atctttaatt ttaatcatgt acatcacccc tcatcacatg tttaaaatga tcattaatct
    14521 tcttcttagt tgataataaa tacttcgtat aaacatgttc tggatgatgc aagactgatt
    14581 cacgcgtacc atgctcaatc agttgaccat ttttcatcac aacaacacgg tctgcaatct
    14641 tgttaataac cgttaaatca tgtgaaatga aaatcatcgc acagtcaaag tgttttttaa
    14701 tatctataaa tgcttccagt acatcatatt gtgtaattgt atctaaagcc gttgtcggct
    14761 catcagcaat gattaacttt ggtttcaaag ctaacgctaa agcaatcatc aatcgctgta
    14821 acatccctcc tgataacatg taaggatatg attttaatat acgtttagga tctttcaaac
    14881 ttaaataatc catatattca atcaatgtct tttcaatttc ttgtgtagac attgacgtat
    14941 gtactttcat agtctcaaac atttgtttac cgacagtagt tgatgggtca aaggcacgac
    15001 taccttgttg catgaccatc gcaatatctt taccacggta ttttttcaat tgtgcttccg
    15061 ataacgacaa cattggctta ccatcaaagg ttatatcacc tgttacgcgt aatcgttctg
    15121 gatttaaacc tacaatcgat ttgcatgtaa ttgatttacc gctaccactt tctccaataa
    15181 caccaagtgt ttcaccttta gttactgtaa aatgaacatc tctaactagc ggttgatctg
    15241 tccatgtgtc tgtaatcgtc aaatgtttaa ctgttaacaa agtcattatt gcaccactcc
    15301 ttttttcaca gaacgaagtt tctctttaga agagatgcgg ggatcaatgg caacttgtaa
    15361 tgaatccgat aagaaattaa atgccattac gataatgact atggcaatac caggtgcaaa
    15421 catcatttca ggatgtgtaa acatcacttt tctcgcttca ttaagcatca taccccactc
    15481 tgcagtaggt gctttgacac ctaatcctaa aaatgaaaag ccagatattt gcaagatcat
    15541 tgaacacatc gaactactag agatgatagc aatatctgct aatgttaacg gcataatatg
    15601 tttgtgaata attttcatat cattcatacc gattgtttta gcaaatctta catggtcaga
    15661 agcagtgtac tgcataacac ttgtacgtat aacacgacag aaccatgccc aacgcgtcaa
    15721 aataaatgcc atgataatat tttcggcacc cataccaaac aatgcaatta atgctaacgt
    15781 tacaacataa cttgggaatg ccaacataac atcacacgca cgcatgatta aggcgtcaac
    15841 aaacccttgg aaatatcctg ataagaatcc taaaatagat ccaataagta cagaaacaaa
    15901 tagcgcaaca aagacatata acaaacttgg tctaatcgca taaattaacc tagttaaaat
    15961 atctctacct aaatggtccg tacctagtag atgttgaaaa ctcatgccag caaatttgtt
    16021 tgctgtatcg atatggttag gatcataaaa tgtcacaagt ggtgctgcta atcctaaaat
    16081 gacatataat acaataatgc ctaatgcgat tactgcaccc ttatcttgta ataaacgttt
    16141 taaaataatc atcgtgcgcc ctcccttaat cttggattta atagcgcatt aatgatatct
    16201 gctaatgtat taaatacaat aaataatacc gctacaatta atacatatgc ttgaatgact
    16261 ggaaaatcat gttcaagtat tgcttttaaa cttaattgac ctagtccagg ccatgcaaag
    16321 atatactcga taacaactag tccacccatt atcattggta tagacataca aaagattgat
    16381 accgcaactt gtaaagcatt acgcaacaca tgcaacatta atgtgataga tttcacaccg
    16441 cttgctctta aataaagtac atagtcttca tttaattgtt ccaccatcga gcgtctaaca
    16501 tttctaaagt aaatgccagc ataggcaatc gtaataacga tcactggcaa tatgtaactt
    16561 tctggacctg ttaatccaga agtcggcaat atgtttaact tcactgaaac gtaaataata
    16621 agtattgaag ctatccaata tgatggtaat gcagttagaa agaaagccac tgaacgtatc
    16681 gcacgatcag tgaactttcc tctttttaat gcactaacta cacctaatat aatcgatgta
    16741 atcattacca taacacttga aattatagtt aatttcaatg tattcataaa tgctgggcca
    16801 atgcgttcag caacaggatc acctgtaatg taacttgtac caaaattaaa ttgcatcgct
    16861 tcaagtaacc agtttttata ttgaattaat aatggatcat tgaaaccata cttttcattc
    16921 gtttctgcaa tcaactctgg ggttacattc ggcgttcctt gtgcatgtaa aattgtcaca
    16981 gctggatttt catttgtaat atacgtcaat agaaatgtca taaaacttac tacaatcact
    17041 aatggaaaca tgagcgcaat acgttttaag ataaatttga acatctaatt gctcctttat
    17101 ttatactgca tttcattgaa tggtaattca tactgtgatt gtgtgaatga tactttatct
    17161 aaatctttcg gtgcaacaac tgtcatgcta ccgtgagaaa tagggataaa gataccttca
    17221 tcatcaattt gtttcaagat gtttttataa gcgtctgaac gttctttacc attttggatt
    17281 ttaaatgcgt catcaatgct gttgtatatt ttatctttgt tctcaattcc tgatgttgca
    17341 ctttcataac cgtttttcgc tttaaatgct gcaatcgtac tttgtggatc gtacaataat
    17401 ccccaagttt ggttgaacat taagtcataa tcaccagaag tacgacgttc agcaatttta
    17461 tctgatgttt cgccattgat gtttagttta atacccattt tcttaaattc tgcttgtaag
    17521 tattctgctt gttctttttg acttgaagaa cctttgtcat agtacattgc catttcaagg
    17581 tttttaccat ctttttgacg aacatcgctg tctttacctt tcttccaacc agcttcatct
    17641 aataatgatt ctgctttttt aaggtcatac ttacgtgttg gcatatcgaa attaatgtct
    17701 gttacatttt tcgcaaataa ttgagttgct ggtttttctt gaccatctaa aatttctttg
    17761 gcaattttat ctctgtttac catatgacca atcgcttgtc tgactgtttt gtcactgaca
    17821 gcgttatctt ttttaccaga attgacaact aacattttcg tattcattgg ttgactacgc
    17881 ttaacttgat agtcacctgt atctttcaat tgttttaaag agtctttgtc taagctatct
    17941 gtacctctat cgtcagtaaa ggcaaagttt gtttcacctt ttttcataga taggaatgct
    18001 gtttcaccag caggcatcac tttagcttgt actttattaa gcttagattt ttcgccccaa
    18061 tattgatcgt ttttgttaaa gtctgcagac tcgtcttttt tatgttcacc taatttaaat
    18121 ggacctgtac catcaaactt tttaacgcca tctttcgttg taccgttttt aaagtctttc
    18181 ggagacacaa atacatatgg acgaggcatc gctagttcag ctaatgctgg ttgatatgct
    18241 tctttcaaac tcaattcaac cgtgtactta tctttaactt ttacattatc aatcaatgtt
    18301 gaaatcttta accaagaatg caatttttta ttttgttgaa ccgcatcaat atttttctta
    18361 actgcatcag catcaaatgg cgttccatca tggaatttca catcatctct taaatggaat
    18421 gtatatgtct tcccatcttc agaaatatcc cattttttag ctagtaacgg tttgatacca
    18481 tctttcgtgt tacgtacaag tggctcataa atcatactct cagcagacat agatccaccg
    18541 taaacatgcg gattcatatc cccgatatct ttaaccgttg tatacgttaa ttgcttattc
    18601 tctttctttt cttctaagcc tttattacca ccacagccag ttaaaattat ccctgatgca
    18661 agtaacatcg cactcatttt agttagtttt ctcattcgtt tttcctcttt ctaaattgat
    18721 aagttgcatt acttaatcaa atcgtaatga ttatttttct caattatttg cttattttta
    18781 tgtaaatgtc aaacataatt caataatttt tttcttttat aaaacttcaa acccttagta
    18841 tgaaagcgtt ctattgattt ccaaaaattt tgtgacgagc gccttatcct cttcaaataa
    18901 gtttgtatta aattgcgaag ataattgttg atcttgatgc atatctttgt acgcctgaca
    18961 ccttgcttca taacgtgtta ggaactgatc aatcattggc gttttgatac ctagcatttt
    19021 cccaatatgc tgaatcatcg ctgttctgta gtagtcttca cttggcattc ttggaatttg
    19081 aacaacatcc tgttcgtttt tatagacttg cttaaatggt accgctgaaa aatcaaagta
    19141 atgtcctttt tcgtctggct gtgaaaatgg atcaatgagg attgcggtat atcttacata
    19201 aagcagatac tcttgtaaaa tatctggcaa gatttcaaaa tgctcaatat caccttcatc
    19261 caaagtttca ggacgtactg gataattctc tttcaccata aattgaagca ggttgactga
    19321 cggcactcta aatgcttgta aaatagccat catctccttc cacattaaac gcatttcacg
    19381 gattagtgtc atcgttatcg gtccttcagg aaataactta tacacataaa ccggtacatc
    19441 cgttccttcg aaaatggctt tcaatgaaaa gtcattcata aatagtggtg ggtgcacata
    19501 aagcgaactg tttcgtgttt ctgcatgcag tggcgattca acgacttcta attgaatatt
    19561 caattgctca gacaaggcat tgatatgttg acacatcgtt gaatttgaat gtgtcgatcc
    19621 catgtacaat ttctttttca caccagttgt caaaacgtga tttggcactt ctttatcaag
    19681 aatacgtgta tcgccaagat aagttgaaaa tgaaatcact tcgatatcct gactgaactt
    19741 agacataaat tgttccacaa tcatttgcga tccaaatgtc ggtgaaatta aaatgacatg
    19801 tttgacactt tgcaatgttt ctaatgacaa ttgttgtaaa gtgtcataat aagcatctgc
    19861 tgtgcatgcc atgacaaccg tttcgtattc acccttaacg ttttttacat ctttatacaa
    19921 acgattaatt tcaaacttac cttcaaggtg tctatgcgcc tcgttctgta ctttaacttc
    19981 aaatcgcttt tcttttttat acgctctata taagcgtttt gatttttctg atgttgaggc
    20041 acgtccaacc atatcaatct cataatctga ttttaaatag caaatgtttg ctaactggat
    20101 tgcgaccgga ccagtgccta tcattaataa tttagacatc cgtaatacct acctttatag
    20161 ctgctttttt ataaagtgct atatcaaaaa tttgctgtgg tctcatatgt ttattcacac
    20221 attgccactt atctttcgtt gtttcttgtg acggataatt aaatagtgct ttgatgccat
    20281 caccaaagcg cattgctact acaacatttt catttgttaa atcatataat tcttctaaaa
    20341 tgctgtactt taaaggaatt gtcgagctga atatgatatg cgtcacatct ttgatatctt
    20401 taagttcaga taccttttga tccgtaattg ttatatcttc atttggtgct aagacgttaa
    20461 cgattctgcg tcctaggtca acggcttgtg gatcaatatc aataccgata actgaagcac
    20521 ctgtttcttt tgctacttga attaatgtca ttggatatgc acctgaacca actaacaata
    20581 atttgtcatg gctagctata ctacattgtc caaactcttc atcaatacaa tgttctatat
    20641 tgtcaaagta acctgtggta gacgcctgtc cgtctaatag acgtctcgct ctaatgactt
    20701 ccacttgttt aacacactgc gctgtaatat attgcagtga ttttattaat tgtgcttttt
    20761 cttcaacgtc acgccaagca ttaaattgtt gttcataaat aggatttaaa ataaattcac
    20821 tatagtcatc catcaatgtt tctaatgctt cgatatattg atcgtcttgt aatacacgct
    20881 cgtaatgcgc ttcaaacttt tctaaatatt gttgtaatat caatttgatt tcattattaa
    20941 agttattcat tgtaggctcc ttattctata tatgcttttc ctgtagctac agttgtaacc
    21001 tgtcctttaa ttgaagtttg atatcccaat tgatgacatc gctttgatgt cactaaaata
    21061 ctgccccctg gctgatgtac tgtaaaatct ttgcatgcgt cattacgttg ataattatta
    21121 aaaaccccaa ttgatgctgt accagaacca cagctatttt cccaaattaa actttggatt
    21181 tctggtatat agattaatgg ctgtaaaaat tgacgttgtt catcaaaaag catcatacct
    21241 actgttttat atttgtcatt ccattgttgt tcacgcacaa atgtttcaac taaatgttga
    21301 atttctgttg ttacttgatt aactggaatc acataatgta catatgtttc ataaataatt
    21361 tctattgctt tccatgaatg attacccatg ttaattgttg ttggcacaac acgatgggct
    21421 tgtggcattt gaacttcata atattggcaa tcatgaattg cgcattgcac taaatctgaa
    21481 caaccagata ctttcacctt aaactgttgg tctttaagta aatgactttc ctgtaaatga
    21541 tgtatatatg acatcgtcgc attaccgcaa aattcattac cgctcattac taagtgaaaa
    21601 ttattgccat catcattttg tgttgattct ataaagccta cctgttcaca acatacatgt
    21661 gttgcggcca ttaactgatt ggcgatagat gcatattcac tagcatcatg ttttgaatga
    21721 acaagtatcg tcatattccc cgaaggatta tacttagaaa attctataac ctgccgattc
    21781 atacgacact cctttagatg tatttttaga gcctctgtaa ttttaattac tagaggctct
    21841 tatgcagttg ttgcgaagca aacaactgta tttctctagc tcttttacca taagtaatta
    21901 gagcctctta tgcagttggt gcgaagcaaa caactgtatt cctctaaacc tgtaattttt
    21961 aattactaga aaaagaacct aatagtttcc catctattag gtaagtcata ctcattcgca
    22021 gaacgaaaag tatgacttag tactcggttg ttgcatcaac atgcaactcc aaacttattt
    22081 aaaagtgctt catgtggctc tattaagtaa atgcaattgt ccgtataact acacccactc
    22141 aaatgcgtat actgttttca cctcaactaa aacttcaaaa aagttgcaga aaacaaatcg
    22201 taataattac gatttgatta tatactatat tagttgagaa tcattgtcaa taaaggttgt
    22261 aaatgaaaat tcatatatcc aaacgagttt cttccttaaa catcacctat tcaagacttt
    22321 agagacattt tgaaaatttt taccaataat tgagtagaaa ttccgttctc gtcagttcaa
    22381 tattttacgt atgttgtctc tacgtattgg gttagtgaga ataacccaca cttgtagaaa
    22441 ctctttgttg taaatgcaca atagacactc ctactaccaa gcctgtaacg gactttcgcc
    22501 gtcaagttgt tgcccatgcc aagcgcacca aaaaagcttt gtacaatttc gtacaaagcc
    22561 ctaaaaattt atataaacat atcattgttt taatgaacta gccatatcag attttatttc
    22621 atttgctaat atttgtaatt caggaaaata attattagca aagccaatta ttattttatt
    22681 ttttacaaac gtattaatct ctcgatcttc tggaatcgta ttgtctatta aattcaaaag
    22741 atcaatatga tttatattct ctatacttac tgtatttaaa tattttaaaa tcgctagata
    22801 caatttatca aatacttcat gttcaaaatc aaagtccaat aataataaac taatggaatt
    22861 agtttctagc tgattatctc caattttatc tgcaagctct cgtatagctt gatataataa
    22921 tctaaaactt tctttttcat ttaacataaa attctcctta acttattaaa atagccaaga
    22981 tacagcttat tttatagata ctcctaaagt ttgaactcga gattctgata aattagatgc
    23041 acgacctaca atacgttaaa acaagcttta agcaatacac ttcaaatgaa caatatgtga
    23101 ttttcaaaga ttaattaatc tccgctttaa atgtctgtca ttgcaacaat atgcatacat
    23161 tgcctacttc atgccaaatt attttccact ggatatcgat tttaaaacaa ttattccaat
    23221 gtacccttcc acgaatcatc actcaacatc tcgccaggcc atgtgtaagc cataacacca
    23281 ccgtcaatac gaattgtttc acctgtaata aatgaactct catcagatgc taaaaagaca
    23341 acgagcttgc ctacttcttc tggtttgcct aaacgtccca gcggtgtcat ccatttctga
    23401 ttttctctaa acgctttgcc ttcctcatct tcgctagtac ctatcagttt atctactaac
    23461 ggggtttcaa tcgtacctgg tgcaatcgca ttagctcgaa tgccatctct accatattcg
    23521 attgcaatgg atttagtaaa attaatcact gcccctttcg cagcattata tcctgagcga
    23581 tacaaatctg ctgcctgtcc tgaaaatgaa gaagtgttaa caatagagcc acctttagtc
    23641 atcattaaag gtaacatcat tttcgtcatt aaaaatgttc cacgcatatc tacattcata
    23701 atcttgtcat atacatctgt tggatactca tgaattctac cagccgcatt atccacaccg
    23761 gcattattaa ataacacatc aacatgccca atctgttctc taatttctga tgcaaaattg
    23821 tcaatttgtt gttcgcttgc aatatccaca atatacgcct tcgcattgtc tccattactt
    23881 tcaatcttat ttacagtctc tgataccgct tcagatatat ctactgccaa tacatacgca
    23941 ccttcttgag ctaaagcgat tgctgaagct tgtcctatac ctgtacttgc ccctgttaca
    24001 actgcaactt tattttccaa acgtttcatt cagtcactcc tatactacat atcttaaact
    24061 tacagtcctt attttccact gttcgatgct tcataaacct aatagataag tcttcaggca
    24121 gtcttaacta ctctctgcta tacaatttct attgaataac tcaaaatatt taacaatatt
    24181 agttttttat tttctgaaaa ttgttacttt taaaagtatt ttttgttaca ctttaaatat
    24241 ttaacaaagg gggaggaata attgatgaca tctaaagaaa gacaaaaagg ttctatcgtc
    24301 aatagtttct taaatagtgt tgaaaaaatt ggaaataaat taccagatcc aagtgtatta
    24361 ttctttttaa tgtgtgtagg tctagcaatt ttgacatggc ttgtctcact atttaatgta
    24421 tccgttaagc atccaggtac acatcaaact atccatgtta aaaatatttt aagccatgat
    24481 ggttttacga tgattatgaa tgatgccata aaaaatttct cagaatttcc tgcattaggt
    24541 ttagtacttg ctgttatgat tggcattggt gttgcagaga aaacaggata cttcgacaag
    24601 ctaatgattt ctgttgtgaa tcgcgcacca cgtttcttaa ttttaccgac tattatttta
    24661 attggcattt taggtagtac agccggcgat gctgcgacaa ttatattgcc accgcttgca
    24721 gcaatgcttt ttattaaaat tggctatcac cctattgctg gtttaacgat ggcatatgct
    24781 tccgctgttg gaggatttgc agcaaatata gttgttggta tgcaagatgc tttagtctat
    24841 tcatttacag atccagcaac acgtatcgtt tcagagtcta ttaaaacaaa cgttgctatg
    24901 aactggtact ttatcgcagc gagcgtcgtt gtattacttc ctacgatatt gctagttaca
    24961 actaaactga ttattcctag gttaggtcaa tatgatgatc gtttaatgca tgatgatcat
    25021 gaagagacgt cttcacatat tactgacaaa gaagcacatg ctttaaaatg ggcaaatatc
    25081 agtttcatag tcacaattat tttattaatt attacagcta ttcctgaaca tagtttttta
    25141 agaaatgcta aaacaggcag cttactagac gatgcgccat taattaacgg cgtcggatta
    25201 attattcttg tcgtattttt agtacctgga ttagtttatg ggattttaag caaagaaatt
    25261 aaaaatacaa aagacttagg taaaatgttc ggcgatgctg taggatcaat gggaacattt
    25321 atcgttattg tgttttttgc agcacagcta cttgcatatt taaagtggag taatttagga
    25381 attatcgctg ctgttaaagg tgccaaatta ttagaacatc aaaacggtat tgtattgata
    25441 ctaggtatta ttgtactgag tgcaatggtc aacatgttaa tcggaagcgc atcggctaaa
    25501 tggggtattt taggaccgat attcgtacca atgttaatcc ttatcggctt tcatcccgca
    25561 ttcacacaag tcatttaccg tgtaggagat tcaattacca atccaattac accgatgatg
    25621 ccgtacttac ctttattatt aacttatgca caaaaatacg ataaacgcat gaaactcgga
    25681 gccttacttt ctagtttaat gccgtattca atcgcgctaa gtattgtgtg gacagtattt
    25741 gtcattattt ggttcttatt aggtatccct gttggaccaa gtggaccgat attcgttaaa
    25801 taacagcatt caaggggata atttaaaatg ccactataca agatttgacc tgtatagtgg
    25861 catgatttgt tatgatatag cggtcgagat gaaactctgt atttatatga acaattatag
    25921 tttatcacta ataatattta gtctgttcca accgttaatt tggtttacaa caaagacaag
    25981 atcaataatt tgttcttcat cataaaatga ttttagccgg tcaataattt caaacttctt
    26041 aaagtctttg attgaattta acatttctgc aaattcaagc gtcacttttt cttgatcagt
    26101 aaataaatct aaatgtctaa aactcaagac ttcatcaatt ttcatttgtg ttacacccaa
    26161 ttcctttaat tcttttttat ggatatcaac acaataatga caaccgttaa tttttgaaat
    26221 taataactta atcaatgcac taagttcatc attaacgggt gattgatgaa taaacttttc
    26281 accttggctc attacagaca ttaactctcc attttgtttc ttgtaattat acggcatttg
    26341 caatctctcc tttacattgt ttaataccaa tccagtgata ctacacttcg aacaatattg
    26401 gatagaattt gaaattctgt ataacatctt gtcaatctat gctttagtta aaacatttag
    26461 cgcttatatt taaaaaatat ataaaatatg aaaacagatt gaaaggatat tataccttgc
    26521 tctacccttt tttataaaaa atcacacaca tttgagaaaa tttgataatg tcaataaacc
    26581 acgtcatcga cattgttatt aggtatatat tgtttttata atggccaaac tattattcca
    26641 tttacattaa tggggttcac ttgtgttaat gtactgtaaa aaactgggga agtattggtg
    26701 atgatgaagt gatttatatt aattgcgaaa caccttcgat agcaatgaca tagattattg
    26761 gaagattaaa tcaaaaataa agaagaccct agtggccttc ttttttatct aattcttctt
    26821 tgatttcgtc aggtgatttt aattgatttg ctggttttag taattcactt aatttttcac
    26881 tttctatcat atctccacca aattgataat tatgttctgg agacccatat gcaacaaaat
    26941 catcttcttt gttttcattt atgtaacctt caattactgc atcgcccata ggtcctcttt
    27001 ttaaacttgt gaaatgtaca gttttgatgg tattgggttc tttggtatta tgcttgagat
    27061 agagtgtaat acgctcttgt tgttctttat agtatattgc tttttgtttt tctttttcat
    27121 catatttcat tttaaaatgg atacctgcaa cactgattaa tatgattaac ataatactag
    27181 ttaatattaa aattttcttt ttcgacatat atcaccagct cctaaaatgt ggtggaattt
    27241 tatacagttt acataattat gatactacat aacacgctct taaaaagtaa aagtagctat
    27301 ttagtaatga ggaaatacta tatttaaaat tgtatgttta tatcaagcta agtttaacgg
    27361 attaaaggga gcaggtctat cacctgttgc taaaacttat agactcaaaa ccaattaatt
    27421 gtatttaata tttgcagaga caaattcgaa ccacttgtta gacttcgagt tttattctgt
    27481 cgaagattca aatacaatat ttgataaaca ttaagcaatt aaaagtgttg atataaagtt
    27541 catctaagat catcggaata ttgcggttat gaagtggtta acagataaat acagtgaaaa
    27601 ttgtactaaa aattcaactt tttattttta tgtgtaagta tgattaactt gaaagcaaaa
    27661 ttaaaacgac cgcatagttt tagcggtcgt tctttttaga ctctaattcc ttttttatct
    27721 catcaggtgt tttggtttga tgtgctggtt ttaataacgt gcttaaattt ttacttttga
    27781 tcaaacttcc accaaattga taatgatgtt ctggtgaccc atatgcaaca aaatcagctt
    27841 ttttgttttc attgatgtat ccttctatta cggcatcgcc cataggtcct gtctttaaaa
    27901 ttgtgaaatg cacagttttg atggtattgg gttctttggt attatgcttg agatagagtg
    27961 taatacgctc ctgttgttct ttgtagtaaa gtgctttttg tttttctttt tcgtcatgtt
    28021 tcacttttaa ataaatgact gtagcaacta tacatataca taaaataaca ccaataatta
    28081 taaaaacatg ttttttggtc attaaagcaa ctcctaaaat gtggtggaaa atgaaaaagt
    28141 ttttatagtt ctgtaattat gcacattata gattataaat cataacacta atatcgtcgc
    28201 cttcactcac tcgattgtta aaatcaccag ttactgagaa ttgtgcaagg aataatttcc
    28261 tatcatgctt ttatatcatt attgccttac taatattaac aaaaccgata gctattacat
    28321 taaatgccta tctcccacac ttcaaacacc tttttataca ggacgcactg taatttcatt
    28381 cacgttaacg tgctttggtt gtgttaatgc atataatact gcttctgcaa tatcttgtgg
    28441 ttcaagtttt ttacggtctg ttggattgta tgtggcagtt attgctgtat ctaccattcc
    28501 tggagaaatg cttgttactt taacacctgt ctttgccaac tctttttcta atccttgagt
    28561 aatagtgtga actgctgctt tcgtcgcact ataaatcgta ctgcttttcg ttacttcaaa
    28621 gccagaaata gatgcaatgt taataagatg gccacttgat tgttctaaca tagttggtaa
    28681 tgcagcctgt gccgtgtata aagtgccttt gatattcaca tcaatcatac tatcccattc
    28741 atctacttga taatcagtaa tcttagacga caacatttgc cccgcactat tgataacaat
    28801 atccaaacca ccgaatgttt gttgtgcaat ttttatcaat tcatcgactt cttctttttt
    28861 cgttacatct gttggcacta ccttcacact atcttgagct aattgattcg ccacattttg
    28921 tagtttatct ttatttctac cggctaagat gactttcaca ccttcttcat gtagtaatgt
    28981 tgcaattgct tctccaatac cactacctgc acctgtaact actgctactt tatctgttaa
    29041 tactgtcata atgatcgact cctttgattc ttttttattt tttcagggta aatcataagt
    29101 acatattact atttatactt ttaaaaagcg tatcacaatt catattctgg ttatactaac
    29161 tcgatttatt tacactgata aagtacattt ttaagttatt cgaaatatac aaatgatcgt
    29221 tgttcatatt ttttattttt aaaaatgttg aaaaattgtc acttctttat tgtaaaagtc
    29281 gaaagttagt actaaagtta taagtattag taaacaatgg caaggctgtt agaaaagggg
    29341 gagttattgt gacatatcat gagcgtgtgt tagcactgag agcagaaagt aaaagaactg
    29401 catttgattt tagattcgag gatttattta gcaaagaaga atggctaagt atgtcgcttg
    29461 cagaaagaca aaaagccgag aaagcatttc gacacgaaat aaaaaaatta gatgatgtaa
    29521 gaatgccctt ctcaagtgca catgatgccc aagtgaaact gtataatgtt gtgtattcat
    29581 acaatggcat taaacgtaat ttcaaacaag ttgaaaatga aggattctaa tatcatttcg
    29641 tatatatatc gaggacatga tacagtttta tatataatct tgtaggtaat catactaaaa
    29701 ttcaatatag acatgactaa agccacatcc aatataggac gtggctttta gtatcgttcc
    29761 agatttgtgg ggagttaggg tttacttaat taaaggggtc tgccctcaat tgctcaccaa
    29821 cgagggcaag ttacatcaat cacacttcaa ttgccgccga ctaggggtag taatcattgg
    29881 caataagaac tagttagtga ctaacgattt acgttccata gcaaagtgat acaaacgttc
    29941 agcatcaatg taaggcatag aatcaatagt taggtaacca tctttgatgt caccaattaa
    30001 tccttgattt acaccagtgt tcacgtaaat atcatagcta cttacgtctg agaaaataag
    30061 cgcttcaatc tcttttctca taatcatcac tccaatgttt atatattatt tatataaact
    30121 ctctcgtctc tctctattta ttaacttctt acaagtctaa tattacatga atttccaaat
    30181 aaataaagag ttttgtgatg tttttcacaa atttatcact atttt
//
LOCUS       DAHHRR010000022        29421 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.022, whole genome shotgun sequence.
ACCESSION   DAHHRR010000022 DAHHRR010000000
VERSION     DAHHRR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29421
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.022"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            104..2551
                     /locus_tag="NBI42_000802"
     CDS             104..2551
                     /locus_tag="NBI42_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HCD1777241.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDY
                     VERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(2660..2758)
                     /locus_tag="NBI42_000803"
     CDS             complement(2660..2758)
                     /locus_tag="NBI42_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777242.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            2892..3182
                     /locus_tag="NBI42_000804"
     CDS             2892..3182
                     /locus_tag="NBI42_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCD1777243.1"
                     /translation="MKESTLEKYLVKEITKQNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            3163..4529
                     /locus_tag="NBI42_000805"
                     /pseudo
     CDS             3163..4529
                     /locus_tag="NBI42_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            4542..4979
                     /locus_tag="NBI42_000806"
     CDS             4542..4979
                     /locus_tag="NBI42_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1777244.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            5136..5450
                     /locus_tag="NBI42_000807"
     CDS             5136..5450
                     /locus_tag="NBI42_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1777245.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     KI"
     gene            5577..5882
                     /locus_tag="NBI42_000808"
     CDS             5577..5882
                     /locus_tag="NBI42_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCD1777246.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSD
                     LMIEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            5872..7563
                     /locus_tag="NBI42_000809"
     CDS             5872..7563
                     /locus_tag="NBI42_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCD1777247.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            7568..8806
                     /locus_tag="NBI42_000810"
     CDS             7568..8806
                     /locus_tag="NBI42_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD1777248.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            8790..9563
                     /locus_tag="NBI42_000811"
     CDS             8790..9563
                     /locus_tag="NBI42_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCD1777249.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            9575..10738
                     /locus_tag="NBI42_000812"
     CDS             9575..10738
                     /locus_tag="NBI42_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCD1777250.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            10807..11085
                     /locus_tag="NBI42_000813"
     CDS             10807..11085
                     /locus_tag="NBI42_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCD1777251.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            11097..11429
                     /locus_tag="NBI42_000814"
     CDS             11097..11429
                     /locus_tag="NBI42_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777252.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            11426..11827
                     /locus_tag="NBI42_000815"
     CDS             11426..11827
                     /locus_tag="NBI42_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777253.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYKEIIKKELAR"
     gene            11828..12223
                     /locus_tag="NBI42_000816"
     CDS             11828..12223
                     /locus_tag="NBI42_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCD1777254.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            12258..12899
                     /locus_tag="NBI42_000817"
     CDS             12258..12899
                     /locus_tag="NBI42_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1777255.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            12991..13446
                     /locus_tag="NBI42_000818"
     CDS             12991..13446
                     /locus_tag="NBI42_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCD1777256.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            13504..13854
                     /locus_tag="NBI42_000819"
     CDS             13504..13854
                     /locus_tag="NBI42_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777257.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            13896..14054
                     /locus_tag="NBI42_000820"
     CDS             13896..14054
                     /locus_tag="NBI42_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777258.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            14068..20267
                     /locus_tag="NBI42_000821"
                     /pseudo
     CDS             14068..20267
                     /locus_tag="NBI42_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            20267..21091
                     /locus_tag="NBI42_000822"
     CDS             20267..21091
                     /locus_tag="NBI42_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCD1777259.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            21100..22683
                     /locus_tag="NBI42_000823"
     CDS             21100..22683
                     /locus_tag="NBI42_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1777260.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            22683..22973
                     /locus_tag="NBI42_000824"
     CDS             22683..22973
                     /locus_tag="NBI42_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777261.1"
                     /translation="MATEEVKIKALLKNDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            22989..24899
                     /locus_tag="NBI42_000825"
     CDS             22989..24899
                     /locus_tag="NBI42_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777262.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            24899..26365
                     /locus_tag="NBI42_000826"
     CDS             24899..26365
                     /locus_tag="NBI42_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCD1777263.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            26365..26754
                     /locus_tag="NBI42_000827"
     CDS             26365..26754
                     /locus_tag="NBI42_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCD1777264.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            26747..26911
                     /locus_tag="NBI42_000828"
     CDS             26747..26911
                     /locus_tag="NBI42_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCD1777265.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            26957..27256
                     /locus_tag="NBI42_000829"
     CDS             26957..27256
                     /locus_tag="NBI42_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCD1777266.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            27392..27694
                     /locus_tag="NBI42_000830"
     CDS             27392..27694
                     /locus_tag="NBI42_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCD1777267.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            27705..29159
                     /locus_tag="NBI42_000831"
     CDS             27705..29159
                     /locus_tag="NBI42_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD1777268.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
BASE COUNT        11237 a         3997 c         5916 g         8271 t
ORIGIN      
        1 agttttcatt ttcgtattta tcataaatga tttcgcgtaa agttttcaaa atttgtcacc
       61 tctttaattt tattaaatta attatacaag aaaggagccg aatatgttag acaaagtcac
      121 tcaaatagaa acaattaaat atgatcgtga tgtctcatat tcttatgctg ctagtcgttt
      181 atccacatat tggactaatc acaatatggc ttggtctgac tttatgcaga agctagcaca
      241 aacagttaga actaaagaag atttaactga gtacaataaa atgtctaagt ctgaacaagc
      301 agatataaaa gatgttggcg gatttgtcgg tggatattta aaagaaggca aacgacgtgc
      361 tggtcaagtc atgaatcgtt caatgctaac acttgatatc gattatgctg ctcaagatat
      421 gactgacata ttatctatgt tttatgattt tgcatattgt ttatattcaa cacataagca
      481 tagagagata agtccaagac tgcgtttagt gattccttta aaacgaaatg taaatgcaga
      541 tgagtatgaa gctattgggc gtaaagtcgc agatatcgtt ggcatggatt acttcgatga
      601 tacaacttat caaccacata ggttaatgta ttggccttca actagcaatg atgcagaatt
      661 tttctttacc tatgaagatt tacctttgtt agatccagat aaaatattaa atgaatatgt
      721 tgattggact gacacattag aatggccaac gtcttcaaag gaagagagta agactaaaag
      781 attagcagat aagcaaggtg acccagaaga aaagccggga attgttggcg cattttgtag
      841 agcctatacg atagaagaag ctatatcaac ttttattcct gacttatacg aaaaacattc
      901 tactaaccgt tatacctatc atgaaggttc aactgcaggt ggattggtgt tatacgaaaa
      961 taacaagttt gcctattctc atcataatac ggatcccgtt agcggtatgc ttgtgaacag
     1021 ttttgattta gtacgcatac acttatatgg tgctcaagat gaagacgcta aaacagatac
     1081 tccggttaat cgactaccta gttataaagc aatgcagcaa agagcgcaaa atgatgaagt
     1141 tgttaaaaag caattaatta acgacaaaat gtctgatgca atgcaggatt tcgatgaaat
     1201 agtaaatagc gatgatgcat ggtctgagac gttagaaatt acttcgaaag gtactttcaa
     1261 agctagtatc ccaaatatag aaattatatt gcgtaatgat ccaaatttaa aaggaaaaat
     1321 agcatttaat gaatttacaa aacaaattga atgcttaggg aaagtgccat ggaatactaa
     1381 ttttaagaca cgtcaatggc aagacggtga tgatagcagt ttaagaagtt atatcgaaaa
     1441 gatttatgac atacaccatt caggtaaaac aaaagatgcc attataagcg tagcaatgca
     1501 aaatgcttat catccagtaa gggattatct aaataaaata tcgtgggatg gacataaacg
     1561 tcttgaaaag ttatttatca aatacttagg tgttgaagat actgaagtga atagaacaac
     1621 taccaaaaaa gcattgactg ctggaattgc tcgagtaatg gagcctggat gtaaatttga
     1681 ctatatgctt acactttatg gtcctcaagg tgtaggtaaa tctgctttgc taaaaaaatt
     1741 aggtggtgca tggttttctg acagtttagt ttctgttaca ggtaaagaag cctatgaggc
     1801 cttacaaggc gtttggctaa tggaaatggc agaacttgca gctacaagaa aagctgaagt
     1861 tgaagctatt aagcatttca tatctaaaca agttgaccgg tttcgtgttg cttatggaca
     1921 ttatattgaa gattttccaa ggcaatgtat tttcattggt acaactaata aagttgattt
     1981 cttaagagat gaaactggtg gaagacgttt ttggccaatg actgtaaatc cagagagagt
     2041 tgaagtgaac tggtctaaac taaccaaaga agagatcgac caaatttggg cagaagctaa
     2101 atattattat gaacaaggag aagagttatt cctcaaccct gaactagaag aagaaatgcg
     2161 ttcaatacaa agcaaacata ctgaggaatc tccatataca ggcattattg atgaatatct
     2221 taacacacca attcctagca attgggatga cttaactatc tttgaacgaa gacgatttta
     2281 tcaaggtgat gttgatatgt taccaacagg aaatgtagat tacgttgaaa gaaataaggt
     2341 ctgtgcgctt gaagtgtttg ttgaatgttt tggtaaagat aagggagata gtagaggatc
     2401 tatggaaatt agaaagattt caaacatctt aagacaatta gacaattggt ctgtatatga
     2461 tggtaataaa agtgggaaaa ttcgatttgg aaaagattat ggtgtacaga tagcttatgt
     2521 aagagatgaa agtttagaag atttaatata ataaatattg aataaatata cattttagag
     2581 tgttgtatca gatgttgcat cattttttga gtgatgcaac acgtgagtgt aaaaagtaat
     2641 cgtaggtgtt gcatcatttt tagtgatgca acattgatgc aacaaatgat acaacacctc
     2701 tttcccttct cgctgtaagg ttcaaccctg tttgtttcca atgttgcatc aaattcacta
     2761 taaagtttaa aaagtagtgt tagggagtaa agaggtatag gggtaaccct ctaacagcta
     2821 tttttaaaag tttggcaaga attgatgcaa catcggaaca caaatataaa ttttgtatac
     2881 aaggtgaata tatgaaagaa tcgacattag aaaaatattt agtgaaagag ataacaaagc
     2941 aaaacggttt atgtttaaaa tgggttgcac ctggaacaag aggtgtgcca gatagaatta
     3001 ttattatgcc agaaggaaaa acatattttg tagaaatgaa gcaagaaaaa ggaaagttgc
     3061 atcctttaca aaaatatgtg catagacaat ttgaaaatag agatcataaa gtatatgtgt
     3121 tatggaataa agaacaagta aatactttta tcagaatggt aggtggaaca tttggcgatt
     3181 gacttcaaac cacatagcta tcaaaagtat gcaatagata aagtgataga taatgagaaa
     3241 tacggtctgt ttttagatat gggtctaggg aaaacagtat caacacttac agcatttagt
     3301 gaattgcagt tgttagacac taaaaaaatg ttagttataa cacctaaaca agttgctaaa
     3361 gatacatggg ttgatgaagt tgataagtgg aaccatttaa atcatctgaa agtgtcttta
     3421 gttttaggaa cacctaaaga aagaaatgat gcattaaaca cagaggctga tatctatgta
     3481 accaataaag aaaatactaa atggttatgt gatcaatata aaaagaatgg ccatttgaca
     3541 tggttgtgat tgatgaactg tctacattta aaagtcctaa gagtcaaagg tttaaatcta
     3601 ttaaaaagaa attaccactc attaatagat ttataggatt aacaggaaca cctagtccaa
     3661 atagtttaca ggatttatgg gctcaagttt atttgataga cagaggtgaa agacttgagt
     3721 cttcattcag tcgttatcga gaaaggtact ttaaaccaac tcatcaagtt agcgaacata
     3781 tttttaagtg ggagctaaga gacggatctg aagaaaagat atataaacaa atagaagata
     3841 tatgtttaag catgaaagcg aaagattatc tggatatgcc tgacagagtt gatactaaac
     3901 aaacagtagt cttatcagaa aaagaaagaa aagtatatga agaattagaa aaaaactata
     3961 ttttagaatc ggaagaagaa ggaacagttg tagctcaaaa tggggcatca ttaagtcaga
     4021 aactacttca actatctaac ggcgcagttt atacagatga ggaagatgta agacttatac
     4081 atgataagaa gttagataag ttagaggaaa ttatagagga gtctcaaggc caaccaatac
     4141 tattgtttta taacttcaaa cacgataaag aaagaatact tcaaaggttt aaggaagcaa
     4201 ccacattaga ggattcaaac tataaagaac gttggaacag tggagacatt aagttgctta
     4261 tagcacatcc agcaagtgca ggacatggat taaacttaca acaaggtggg cacattattg
     4321 tttggtttgg acttacatgg tccttggaat tataccaaca agcaaatgct agattataca
     4381 gacaaggaca aaatcatacg actattattc atcatatcat gaccgataac acaatagatc
     4441 aaagagtata taaagcttta caaaataaag aactaacgca agaagaatta atgaaagcta
     4501 ttaaagcaag aatagctaag cataagtaat ggaggtctaa catggggaac acaatatatg
     4561 atatcaagcc aggaacattt aaatatattg aatcagaaat atataattta aatgagaaca
     4621 agaaagaaat aaaaagattg aggttggaaa tacttaatcc aacgaaggaa caagattcca
     4681 atattgtata tggaccatta caaaaaggcg aaccagttag aacaactgaa ctaatggcaa
     4741 ctagattatt aactaataag atgttacgaa accttgaaga aatggtcgaa gcagttgaga
     4801 gtgaatactt aaagttgcct gaagatcata agaaagtaat aaggctcaag tattggaata
     4861 aagaaaagaa gttaaagatg gaacagatag gacatgaatg ccatatgcat cgtaatactg
     4921 ttactactat acgaaagaac tttgttaaag cggtagcgta tcatgcaggt atcaaataac
     4981 attgtgcaaa gattgtgcaa aaggcctaca aatctgtagt aatatgatag tatcggaaag
     5041 atgtataaag ttatctaaaa gttatacgac acaagtacac gaggcacatc gctatgcggt
     5101 gtgtcttttg ttatgcaatc aaagaggtgt aagagatgac caagcacaat aacatttata
     5161 agcatggtcg taagtcatat caatacgatt ggttctatca ttcaaaagca tggaagaagt
     5221 taagggagat agcattagat agagataatc atctttgtca aatgtgttta cgcgaagata
     5281 ttgtaacaga tgcaaacata gtgcatcaca ttatttatgt tgatgaagat tttaacaaag
     5341 ctttagaatt agataatcta atgtcagttt gttatggctg tcataacaaa attcatgcaa
     5401 atggtaatga caaaagtaat cttaagaaaa ttagagttct aaaaatttaa ataaaaaaac
     5461 atttaaataa aattttatag ccccctgccc attggcttaa aatgtttttt cgccgggtac
     5521 cggcgggggc ccttcgcttg caacgcggat aaacttttat gaaagggggt ctttatatga
     5581 aattaacaaa aaaacagctg aaagaatata tagaggatta taaaaaatct gatgacatat
     5641 taattaattt gtatatagaa acgtatgaat tttattgtcg gttaagagat gaacttaaaa
     5701 atagtgattt gatgatagag catacaaaca aggctggtgc gagcaatatt gttaagaatc
     5761 cattaagcat agaactgaca aaaacagttc aaacactaaa taacttactc aagtctatgg
     5821 gtttaactgc agcacaaaga aaaaagatag ttcaagaaga aggtggattc ggtgactatt
     5881 aaagttttaa atgaaccttc accaaaacta ttaacaacat ggtatgcaga gcaagtcact
     5941 caagggaaaa taaaaacaag caaatatgtt aaaaaagaat gtgagagaca ccttagatat
     6001 ctagaaaatg gaggtaaatg ggtatttgat gaagaattag cgcaccgtcc tattcgattc
     6061 atagaaaagt tttgtaaacc ttccaaagga tctaaacgtc aacttgtatt acaaccatgg
     6121 caacatttta ttattggcag tttgtttggt tgggttcata aagaaacaaa actgcgcagg
     6181 tttaaagaag ctttgatatt tatggggcga aaaaatggta aaacaactac tatatctggt
     6241 gttgctaact atgctgtttc tcaagatgga gaaaacggcg ctgaaatcca tcttttagca
     6301 aacgtaatga aacaagctag aatattattc gatgaatcta aggcgatgat aaaagctagc
     6361 ccaaagcttg ataaaaattt cagaacatta agagatgaaa tccattatga cgcaacgata
     6421 tcaaaaatta tgccccaagc atcagatagc gataagttag atggattgaa tacacacatg
     6481 gggatttttg atgaaattca tgaatttaaa gactataaat tgatttcagt tataaaaaac
     6541 tcaagagctg caaggttaca acctcttctc atctacatta cgacagcagg gtatcaatta
     6601 gatggtccac ttgttgatat ggtagaagcg ggaagagaca ccttagatca aatcatagaa
     6661 gacgaaagaa ctttttatta tttagcatct ttggatgatg acgatgatat taatgattcg
     6721 tcgaactgga taaaagcaaa tcccaactta ggtgtctcta taaatttaga tgagatgaaa
     6781 gaagagtggg aaaaagctaa gagaacacca gctgaacgtg gagattttat aaccaaaagg
     6841 tttaatatct ttgctaataa tgacgagatg agttttattg attacccaac actccaaaaa
     6901 aataatgaaa ttgtttcttt agaagagctg gaaggcagac catgcacgat tggttatgat
     6961 ttatcagaaa cagaggactt tacagccgcg tgtgctactt ttgcgttaga taatggtaaa
     7021 gttgcagttt tatcgcattc atggattcct aagcacaaag ttgaatattc taacgaaaaa
     7081 ataccctata gagaatggga agaagatggc ttattaacag tgcaagataa gccttatatt
     7141 gactaccaag atgttttaaa ttggataatt aagatgaatg agcattatgt agtagaaaaa
     7201 attacttatg atagagcgaa cgcattcaaa ctaaatcaag agttaaaaaa ttacgggttt
     7261 gaaacggaag aaacaagaca aggagctttg accttgagcc ctgcattgaa ggatttaaaa
     7321 gaaatgtttt tagatgggaa aataatattt aataataatc ctttaatgaa atggtatatc
     7381 aataatgttc agttgaaact agacagaaac ggaaactggt tgccgtctaa gcaaagcaga
     7441 tatcgtaaaa tagatggctt tgcagcattt ttaaacacat atacagatat tatgaataaa
     7501 gttgtttctg acaagggtga aggaaacata gaatttatta gtattaaaga tataatgcgt
     7561 taaggaggtg aatgttatcg caaaagagaa tattgtcaca cgcataaaga aaaaattgat
     7621 agacaattgg attgatcagt cagcttctaa gctttatgac tttagcccat ggaaaaataa
     7681 atctttttgg ggtgtaatca ataatacgct tgaaactaat gaaacgatat tttcagctat
     7741 tacaaagtta tctaattcga tggctagttt gcccttgaaa atgtatgaag attataaagt
     7801 agttaataca gaagtatctg atttacttac agtgtcaccg aataattctc tgagcagttt
     7861 tgattttatt aatcaaattg aaacaatcag aaatgaaaaa ggtaatgcat atgtgctaat
     7921 tgaacgagac atctatcatc aaccatcaaa gcttttctta ttaaatccag atgttgttga
     7981 aatgttaatt gaaaaccaat cacgtgaact ttattattcc attcatgctg caactggaaa
     8041 taaattgatt gttcataata tggacatgtt gcattttaaa cacatcgtgg catctaatat
     8101 ggtgcaaggc attagtccga ttgatgtgtt gaagaataca actgattttg ataatgcagt
     8161 aagaaccttt aatcttacag aaatgcaaaa acctgattct ttcatgctta aatatggttc
     8221 caatgtaggt aaagaaaaaa ggcagcaagt gttagaagat ttcaaacagt actatgaaga
     8281 aaacggtgga atattattcc aagagcctgg tgttgaaatc gaaccgttac ctaaaaaata
     8341 tgtctctgaa gatatagtgg caagcgagaa tttaacaaga gaaagagtag ctaacgtttt
     8401 tcaattgccc tcagtattct taaatgcaag atcaaataca aatttcgcga aaaatgaaga
     8461 gttaaacaga ttttacttgc agcatacctt attgccaatc gtcaaacagt atgaagaaga
     8521 atttaatcgg aaactactta ctaaaacaga cagagaaaaa aataggtatt ttaaatttaa
     8581 cgttaaatct tatttaaggg ctgatagtgc aacacaagca gaagtgtact ttaaagcagt
     8641 tcgtagtggt tactacacta taaatgacat tagagagtgg gaagatttac caccagttga
     8701 aggtggagat aagccgctaa taagcggtga tttataccca attgacacgc cacttgaatt
     8761 aagaaaatct ttgaaaggtg gtgataaaaa tgtcaatgaa agctaagtat tttcaaatga
     8821 aaagaaaatc aaaaagtaaa ggtgaaatat ttatttatgg tgatattgta agtgataaat
     8881 ggtttgaaag tgatgtaact gctacagatt tcaaaaataa actagatgaa ctaggagaca
     8941 tcagtgaaat agatgttcat ataaattcat ctggaggcag tgtatttgaa gggcatgcaa
     9001 tatacaatat gctaaaaatg catcctgcaa aaattaatat ctatgtcgat gccttagcgg
     9061 catcaattgc tagtgttatc gctatgagtg gtgacactat ttttatgcac aaaaatagtt
     9121 ttttaatgat tcataattca tgggttatga ctgtaggtaa tgcagaagaa ttaagaaaga
     9181 cagcggattt acttgaaaaa acagatgctg ttagtaattc agcttattta gataaagcaa
     9241 aagatttaga tcaagaacac ttaaaacaga tgttagatgc agaaacttgg cttactgcag
     9301 aagaagcctt gtctttcggc ttgatagatg aaattttagg agctaatgaa atagctgcta
     9361 gtatctctaa agagcaatat aagcgtttcg agaacgtccc agaagattta aagaaagatg
     9421 tagacaaaat cactaaaatt gatgatgtag atacatctga attggttgaa acacctaaag
     9481 aaagtatgtc actagaagaa aaagaaaaaa gagaaaaaat taaacgcgaa tgcgaaattt
     9541 taaaaatgac aatgaattat taggaggaaa tgaaatgccg acattatatg aattaaaaca
     9601 atccttaggt atgattggac aacaattaaa aaataaaaat gatgaattga gtcagaaagc
     9661 aacagatcca aatattgata tggaagacat caaacaacta gaaacagaaa aagcaggttt
     9721 acaacaaaga tttaacattg ttgaaagaca agtgcaagac attgaagaga aagaaaaagc
     9781 gaaagttaaa gataaaggag aagcttatca atctttaagt gataatgaga agatggttaa
     9841 agctaaggca gagttttatc gtcacgcgat tttaccaaat gaatttgaaa aaccttcaat
     9901 ggaggcacaa cgtttattac acgctttacc aacaggaaat gattcaggtg gagataagct
     9961 cttaccaaaa acactttcta aagaaattgt ttcagaacca tttgctaaaa accaattacg
    10021 tgaaaaagct cgtctaacta acattaaagg tttagagatt ccaagagttt catacacttt
    10081 agacgatgat gatttcatta cagacgtaga aacagcaaaa gaattaaaag caaaaggtga
    10141 tacagtcaag ttcactacta ataaattcaa agtatttgct gcaatttcag atactgtaat
    10201 tcatggatca gatgtagatt tagtaaactg ggttgaaaac gcactacaat caggattagc
    10261 agctaaagag cgtaaagatg ccttagcagt aagtcctaaa tctggattag aacacatgtc
    10321 attttataat ggatctgtta aagaagttga gggagcagac atgtatgatg ctattattaa
    10381 cgctttagca gatttacatg aagattatcg tgataacgca acaatttata tgcgatatgc
    10441 agattatgtc aaaattatta gtgttctttc aaatggaaca acaaatttct ttgacacacc
    10501 agcagaaaaa gtatttggca aaccagtagt atttacagat gcagcagtta aacctattgt
    10561 gggagatttc aattattttg gaattaacta tgatggaaca acttatgaca ctgataaaga
    10621 tgttaaaaaa ggcgaatatt tgtttgtatt aacagcatgg tatgatcagc aacgtacatt
    10681 agacagtgca ttcagaattg caaaagcaaa agaaaataca ggtccattac ccagctaagc
    10741 cccaaaaggt taatgtaaca gctaaggcta aatcagctgt aatatcagcc gaataggggt
    10801 gatgaaatga gtttagaaga aattaaattg tggttgagaa ttgactataa tttcgaaaat
    10861 gatttaattg aaggtctcat tcaatcggct aagtctgaat tactattaag tggggttcca
    10921 gattatgaca aagatgactt ggaatacccg cttttttgta cagcgattaa atatatcatt
    10981 gcaagagatt atgaaagtcg tggatactca aatgaccaat ctagaagcaa ggtgtttaat
    11041 gaaaaaggat tgcaaaaaat gattttgaaa ttaaaaaagt ggtaggtgat ttttaaatgg
    11101 aatttaatga atttaaagat cgcgcgtatt tttttcaata tataaacaaa ggaccatatc
    11161 cagatgaaga ggaaaaaatg aaattgtata gttgcttttg taaaatttat aatccttcta
    11221 tgaaagatag agaaatttta aaagcgactg aatcaaaatc aggattaacc ataattgtca
    11281 ggtcttctaa aactgaatat ctaccacaaa caaatcactt agttaaaatt gacagtgcat
    11341 tatattccga taaattattc aacattgtag aaataagaat tgatacacca gatattggct
    11401 ataatacagt ggttttatca gaaaaatgag tgtagaaatt aaagggatac ctgaagtgtt
    11461 gaataaatta gaatcggtat acggtaaaca agcaatgcag gctaagagtg ataaagcttt
    11521 aaatgaagca tctgaatttt ttataaaggc tttaaagaaa gagttcgaga gctttaaaga
    11581 tacgggtgcc agtatagaag aaatgactaa atctaagcct tatacaaaag ttggcagtca
    11641 agaaagggct gttttaattg aatgggtagg ccctatgaat cgcaaaaaca ttattcactt
    11701 gaatgaacat ggttatacaa gagatggaaa aaaatataca ccaagaggtt ttggagttat
    11761 tgcaaaaaca ttagctgcta gcgaacgtaa gtataaagaa attataaaaa aggagttggc
    11821 cagataaatg aatatattaa acaccataaa aggaatttta ttatctgatg cagagctcaa
    11881 aacacatata aattctagaa tatactatta caaagtcact gaaaacgctg aaacttccaa
    11941 accctttgtt gttattacac ctgtttatga tttgccttca gactttatgt ctgataaata
    12001 tctcagtgaa gaatacttaa ttcaaataga tgtagaatct tcaaataatc agaaaacaat
    12061 tgatataaca aaacgaataa gatacctgtt atatcaacaa aatttaattc aagcatctag
    12121 tcagttagat gcttattttg aagaaactaa acgttatgtg atgtcgagac gatatcaagg
    12181 catacccaaa aatatatatt ataaaaatca gcgcatcgaa taggtgtgct ttttaatttt
    12241 taaggaggaa ataagcaatg gcagaaggac aaggttctta taaagtaggt tttaaaagat
    12301 tatacgttgg agtttttaac ccagaagcaa caaaagtagt taaacgcatg acatgggaag
    12361 atgaaaaagg tggtacagtt gacctaaata tcacaggttt agcaccagat ttagtagata
    12421 tgtttgcatc taacaaacgt gtatggatga aaaaacaagg tactaatgaa gttaagtctg
    12481 acatgagtat tttcaatatt ccaagtgatg atttaaacac agttattgga cgtactaaag
    12541 ataaaaatgg tacatcttgg gtaggagaga atacaagagc accgtatgta acagtaattg
    12601 gcgaatcgga agatggttta acaggtcagc cggtatatgt agccttactt aaaggtactt
    12661 ttagtttaga ttcaattgaa tttaaaacac gaggtgaaaa agcagaagcc ccagaaccta
    12721 caaaattaac aggtgactgg atgaatagaa aagttgatgt tgatggaacg tcacaaggta
    12781 ttgtatacgg ttatcatgaa ggtaaagaag gagaagcaga attcttcaaa aaagtattcg
    12841 ttggatacac ggacagtgaa gatcattcag aggattctgc aggttcgtta cccagctaat
    12901 ccccaaaatg ttgaagtagc agttaattca aaatctgcaa cagtttcagc agaatagggg
    12961 ctttcaaaat aaatcaaagg agaataattt atgactaaaa ctttaaaggt ttataaagga
    13021 gacgacgtcg tagcttctga acaaggtgaa ggcaaagtgt cagtaacttt atctaattta
    13081 gaagcggata caacttatcc aaaaggtact taccaagtgg catgggaaga aaatggtaaa
    13141 gaatctagta aagttgatgt acctcaattc aaaaccaatc caattctagt ctcaggcgta
    13201 tcatttacac cagaaactaa atcaattatg gtaaataccg atgacaatgt tgagccaaac
    13261 attgcaccaa gcacagcaac gaataaaata ttgaaatata caagtgaaca tccagaattt
    13321 gttactgtag atgaaaatac aggagcaatt cacggtgtag ctgaaggtac ttcagtaatc
    13381 actgctatgt ctactgatgg aagcgataag tcaggacaaa tttcagtgac agtaacaaac
    13441 ggatagggat ttaaggcgca gtatatctgc gtctttttta tttgaataaa aggagctaat
    13501 acaatgatta aatttgaaat taaagatcgt aaaacaggaa aaacagagag ctatacaaaa
    13561 gaagatgtaa caatgggcga agcagaaaaa tgctatgagt atttagaatt agtaaatcaa
    13621 gagaataaaa aagaagcacc taacgcaaca aaaatgagac aaaaagagcg acagttatta
    13681 gtagatttat ttaaagatga aggattgact gaagaagatg ttctgaacaa gatgagtact
    13741 aaaacttata caaaagcctt acaagatata tttcgagaaa tcaatggtga agatgaagaa
    13801 gattcagaaa ctgaaccaga agagatggga aagacagaag aacaatctca ataaaagaca
    13861 ttttatcgaa cattaagaaa atacaacgtt tctgtatgga gcagtatggg tggacattaa
    13921 ctgaagtcag aaaacagccg tatgtaaaac ttttagaaat acttaatgaa gagaataaag
    13981 aagagactga agaaaaacaa agtgaacaaa aagtcattac aggtacggat ttaagaaaac
    14041 tttttggaag ctagaaagga ggttaatatg aatgaaaaag tagaaggcat gaccttggag
    14101 ctgaaattag accatttagg tgtccaagaa ggcatgaaag gtttaaagcg acaattaggt
    14161 gttgttaata gtgaaatgaa agctaatctg tcagcatttg ataagtctga aaaatcaatg
    14221 gaaaaatatc aggcgagaat taaggggtta aatgataggc ttaaagttca aaaaagatgt
    14281 attctcaagt agaagatgag cttaaacaag ttaacgctaa ttaccaaaaa gctaaatcca
    14341 gtgtaaaaga tgttgagaaa gcatatttaa agttagtaga agccaataaa aaagaaaaat
    14401 tagctcttga taaatctaaa gaagccttaa aatcatcgaa tacagaactt aaaaaagctg
    14461 aaaatcaata taaacgtaca aatcaacgta aacaagatgc gtatcaaaaa cttaaacagt
    14521 tgagagatgc agaacaaaag cttaagaata gtaaccaagc tactactgca caactaaaaa
    14581 gagcaagtga cgcagtacag aagcagtccg ctaagcataa agcacttgtt gaacaatata
    14641 aacaagaagg caatcaagtt caaaaactaa aagtgcaaaa tgacaatctt tcaaaatcaa
    14701 atgataaaat tgaaagttct tacgctaaaa ctaatactaa attaaagcaa acagaaaaag
    14761 aatttaatga tttaaacaat actattaaga atcatagcgc taatgtcgca aaagctgaaa
    14821 cagctgttaa taaagaaaaa gctgctttaa ataatttgga gcgttcaata gataaagctt
    14881 catccgaaat gaagactttt aacaaagaac aaatgatagc tcaaagtcat ttcggtaaac
    14941 ttgcaagtca agcggatgtc atgtcaaaga aatttagttc tattggagac aaaatgactt
    15001 ccctgggacg tacaatgacg atgggcgtat ctacaccaat tactttaggg ttaggtgcag
    15061 cattaaaaac aagtgcagac tttgaaggcc aaatgtctcg agttggagcg attgcgcaag
    15121 caagcagtaa agacttgaaa agcatgtcta atcaagcagt tgacttagga gctaaaacca
    15181 gtaaaagtgc taacgaagtt gctaaaggta tggaagaatt ggcagcttta ggctttaatg
    15241 ccaaacaaac aatggaggct atgccaggtg ttatcagtgc agcagaagca agtggtgcag
    15301 aaatggctac aactgcaact gtaatggctt cagcgattaa ctctttcggt ttaaaagcat
    15361 ctgatgcaaa tcatgttgct gatttacttg cgagatcagc aaatgatagt gctgcagata
    15421 ttcagtacat gggagatgca ttgaagtatg ctggtactcc tgcaaaagca ttaggagttt
    15481 caatagagga cacttccgca gcaattgaag ttttatctaa ctcaggttta gagggttctc
    15541 aagcaggtac tgccctaaga gcttcattta tcaggctagc taatccaagt aaaaatacag
    15601 ctaaggaaat gaaaaaatta ggtattcatt tgtctgatgc taaaggtcaa tttgttggca
    15661 tgggtgaatt gattagacag ttccaagata atatgaaagg catgacgaga gaacaaaaac
    15721 tagctacagt ggctacaata gttggtactg aagcagcaag tggattttta gccttgattg
    15781 aagcgggacc agataaaatt aatagctata gtaaatcctt aaagaattcc aatggcgaaa
    15841 gtaaaaaagc agcagatttg atgaaagata atctcaaagg cgctctggaa caattaggtg
    15901 gcgcttttga atcattagca atcgaagtcg gtaaagattt aacgcctatg attagagcag
    15961 gagcggaagg tttaacaaaa ttagttgatg gatttacaca tctccctggt tgggttagaa
    16021 aagcttcagt aggattagca ctttttggtg cagcaattgg acctgcagtt cttgctggag
    16081 ggttattaat acgtacagtt ggaagtgctg ctaaaggata tgcgtcatta aatagacgta
    16141 ttgctgaaaa tacaatcctt tcaaatacta attcaaaagc aatgaaatct ttaggtcttc
    16201 aaacattatt tcttggttct acaacaggaa aaacgtcaaa aggctttaaa gggttagccg
    16261 gagctatgat gtttaattta aaacctataa atgttttgaa aaattctgca aagctagcaa
    16321 ttttaccgtt caaacttttg aaaaacggtt taggattagc tgcaaaatct ttatttgcag
    16381 taagtggagg cgcaagattt gcgggtgtag ccttaaggtt tttaacagga cctataggtg
    16441 ctacaataac tgctattaca attgcgtata aagtttttaa aaccgcatat gatcgtgtgg
    16501 aatggttcag aaacggtatt aacggtttag gagaaactat aaagtttttt ggtggtaaaa
    16561 ttattggcgg cgctgttaga aagctaggag agtttaaaaa ctatcttgga agtatcggca
    16621 aaagcttcaa agaaaagttt tcaaaagata tgaaagatgg ttataaatca ttaagcgacg
    16681 atgaccttct caaagtagga gtcaacaagt ttaaaggatt tatgcaaacc atgggcacag
    16741 cttctaaaaa agcgtctgat actgtaaaag tgttagggaa aggtgtttca aaagaaacag
    16801 aaaaagcttt agaaaaatat gtgcattatt ctgaagaaaa tagcagaatc atggaaaaag
    16861 tacgtttaaa ctcgggtcag atatcagaag acaaagcaaa aaaacttttg aaaattgaaa
    16921 cggatttatc taataacctt atagctgaaa tagaaaaaag aaataaaaag gaactcgaaa
    16981 aaactcaaga acttattgat aagtatagtg cattcgatga acaagaaaag caaaacattt
    17041 taactcgaac taaagaaaaa aatgacttgc gaattaaaaa agagcaagaa ctcaatcaga
    17101 aaatcaaaga attgaaagaa aaagctttga gtgatggtca gatttcagaa aatgaaagaa
    17161 aagaaattga aaagcttgaa aatcaaagac gtgatatcac tgttaaagaa ttgagtaaga
    17221 ctgaaaaaga gcaagagcgt attttagtaa gaatgcaaag aaacagaaat gcttattcaa
    17281 tagacgaagc gagcaaagca attaaagaag cagaaaaagc aagaaaagca agaaaaaaag
    17341 aagtggacaa gcaatatgaa gatgatgtca ttgctataaa aaataacgtc aacctttcta
    17401 agtctgaaaa agataaattg ttagctattg ctgatcaaag acataaagat gaagtaagaa
    17461 aagcaaaatc taaaaaagat gctgtagtag atgttgttaa aaagcaaaat aaagatattg
    17521 ataaagaaat ggatttatcc agtggacgtg tatataaaaa tactgaaaag tggtggaatg
    17581 gccttaaaag ttggtggtct aactttagag aagaccaaaa gaaaaaaagc gataaatacg
    17641 ctaaagaaca agaagaaaca gctcgtagaa acagagaaaa tataaagaaa tggtttggaa
    17701 atgcttggga cggcgtaaaa agtaaaactg gcgaagcctt tagtaaaatg ggcagaaatg
    17761 ctaatcattt tggcggcgaa atgaaaaaaa tgtggagcgg aatcaaaggg attccaagca
    17821 aattaagttc aggttggagc tcagccaaaa gttctgtagg ataccacact aaggctatag
    17881 ctaatagtac tggtaaatgg tttggaaaag cttggcaatc tgttaaatcg acaacaggaa
    17941 gtatttacaa tcaaactaag caaaagtatt cagatgcttc agataaagct tgggcgcatt
    18001 caaaatctat ttggagaggc acatcaaaat ggtttagcaa tgcatataaa agtgcaaagg
    18061 gctggctaac ggatatggct aataaatctc gcgcgaaatg ggataatatt tctagtacag
    18121 cttggtcgaa tgcaaaatcc gtttggaaag gaacatcgaa atggtttagt aactcataca
    18181 aatctttaaa agattggact ggggatatgt attcaagagc ccacgatcgt tttgatgcaa
    18241 tttcaagttc ggcatggtct aacgctaaat cagtatttaa tggttttaga aaatggctat
    18301 ccaaaacata tgattggatt agagatattg gtaaagacat gggaagagct gcggctgatt
    18361 taggtaaaaa tgttgctaat aaagctattg gcggtttgaa tagcatgatt ggcggtatta
    18421 ataaaatatc taaagccatt actgataaaa atctcatcaa gccaatacct acattgtcta
    18481 ctggtacttt agcaggaaag ggtgtagcta ccgataattc gggagcatta acgcaaccga
    18541 catttgctgt attaaatgat agaggttctg gaaacgcccc aggtggtgga gttcaagaag
    18601 taattcacag ggctgacgga acattccatg caccccaagg acgagatgtg gttgttccac
    18661 taggagttgg ggatagcgta ataaatgcta atgacactct gaagttacag cgtatgggtg
    18721 ttttaccaaa gtttcatgga ggtacgaaaa agaaagattg gctagaccaa cttaaaggta
    18781 atataggtaa aaaagcagga gaatttggag ctacagctaa aaacacagcg cataatatca
    18841 aaaaaggtgc agaagaaatg gttgaagcag caggcgataa aatcaaagat ggtgcatctt
    18901 ggttaggcga taaaatcggc gatgtgtggg attacgtaca acatccaggg aaactagtaa
    18961 ataaagtaat gtcaggttta aatattaatt ttggaggcgg agctaacgct acagtaaaaa
    19021 tagctaaagg cgcatactca ttgctcaaaa agaaattagt agacaaagta aaatcgtggt
    19081 ttgaagattt cggtggtgga ggcgatggaa gctatctatt tgaatatcca atctggcaaa
    19141 gatttggacg ctacacaggt ggacttaact ttaatggcgg tcgtcactat ggtatagact
    19201 ttggtatgcc ttctggaaca aacgtttatg ccgttaaagg tggtatagca gataaggtat
    19261 ggactgatta cggtggcggt aattctatac aaattaagac tggtgctaat gaatggaact
    19321 ggtatatgca tttatctaag caattagcaa gacaaggcca acgtattaaa gctggtcaac
    19381 tgatagggaa atcaggtgct acaggtaatt tcgttagagg agcacactta catttccaat
    19441 tgatgcaagg gtcacatcca gggaatgata cagctaaaga tccagaaaaa tggttgaagt
    19501 cacttaaagg tagtggcgtt cgaagtggtt caggtgttaa taaggctgca tctgcttggg
    19561 caggcgatat acgtcgtgca gcaaaacgaa tgggtgttaa tgttacttcg ggtgatgtag
    19621 gaaatattat tagcttgatt caacacgaat caggaggaaa tgcaggtata actcaatcta
    19681 gtgcgcttag agacatcaac gttttacagg gcaatccagc aaaaggattg cttcaatata
    19741 tcccacaaac atttagacat tatgctgtta gaggtcacaa caatatatat agtggttacg
    19801 atcagttatt agcgttcttt aacaacagat attggcgctc acagtttaac ccaagaggtg
    19861 gttggtctcc aagtggtcca agaagatatg cgaatggtgg tttgattaca aagcatcaac
    19921 ctgctgaagt gggtgaagga gataaacagg agatggttat ccctttaact agacgtaaac
    19981 gagcaattca attaactgaa caggttatgc gcatcatcgg tatggatggc aagccaaata
    20041 acatcactgt aaataatgat acttcaacag ttgaaaaatt gttgaaacaa attgttatgt
    20101 taagtgataa aggaaataaa ttaacagatg cattgattca aactgtttct tctcaggata
    20161 ataacttagg ttctaatgat gcaattagag gtttagaaaa aatattgtca aaacaaagtg
    20221 ggcatagagc aaatgcaaat aattatatgg gaggtttgac taattaatgc aatcttttgt
    20281 aaaaatcata gatggttaca aggaagaagt aataacagat tttaatcagc ttatattttt
    20341 agatgcaagg gctgaaagtc caaacaccaa tgataacagt gtaactatta acggagtaga
    20401 tggtatttta ccgggcgcaa ttagttttgc gcctttttca ttagtattaa ggtttggcta
    20461 tgatggtata gatgttatag atttaaattt atttgagcat tggtttagat ctgtgtttaa
    20521 tcgcagacat ccttattatg ttattacttc tcaaatgcct ggtgttaaat atgcagtgaa
    20581 tacagctaat gttacatcta atttaaaaga tggttcttca actgaaattg aagtaagttt
    20641 aaatgtttat aaagggtatt ctgaatcagt taattggacc gatagcgagt tcttattcga
    20701 ctctaattgg atgtttgaaa atggaattcc tcttgatttc acacctaaat atactcatac
    20761 atcaaatcaa tttactattt ggaacggttc tactgatacg ataaatccac gattcaagca
    20821 cgatttgaaa atattaatta atttaaatgc gagtggagga tttgaactgg ttaactatac
    20881 aacaggtgat atttttaagt acaacaaaag tatagataaa aacactgatt ttgttttaga
    20941 tggtgtgtat gcatatcgag atataaatag agtgggaatt gatacaaata gaggcattat
    21001 aacattagcg ccaggtaaaa atgaatttaa gattaaagga gacgtcagtg atattaaaac
    21061 tacatttaag tttcctttta tttataggta ggtgatttaa tggattatca tgatcattta
    21121 tcagtaatgg attttaatga attgatttgt gaaaatttac tagatgtaga ttatggttct
    21181 tttaaagaat attatgaact gaatgaagct aggtacatca cctttacagt ttatagaact
    21241 actcataata gttttgtttt tgatttattg atttgtgaaa acttcataat ttatcatggt
    21301 gaaaaatata caattaagca gacagcgcca aaggttgaag gtgataaagt ttttattgaa
    21361 gttacggcat atcacataat gtatgaattt caaaatcact cagtggaatc aaataagctt
    21421 gatgacgaca gtagcgaaac tggtaaaacg ccagaatact ctttagatga gtacttaaga
    21481 tatggatttg caaatcaaaa aacttcggtc aaaatgacct ataaaataat tggagatttt
    21541 aagcgaaaag taccgattga cgaattaggt aacaaaaacg gcttagaata ctgtaaagaa
    21601 gcggtagacc tgtttggctg tataatttac ccaaatgata cagagattgg tttttattct
    21661 cctgaaacat tttatcaaag aagcgagaaa gtgattcgat atcaatataa tactgatact
    21721 gtatctgcaa ctgtcagtac attggaatta agaacagcta taaaagtttt tggaaaaaag
    21781 tatacagctg aggaaaagaa aaattataat cctattagaa caactgacat taaatattca
    21841 aatggtttta taaaagaagg tacttatcgt accgaaacaa ttgggtctaa agctactatt
    21901 aactttgatt gcaagtatgg taatgaaaca gttagattta caataaaaaa gggctctcaa
    21961 ggtggaatat ataagttgat tttagacggc aagcaaatta agcaaatttc ttgttttgct
    22021 aagtcggttc agtctgaaac aatagattta ataaaaaata ttgataaagg caagcacgtt
    22081 ttagaaatga tatttttagg agaagacccc aaaaatagaa ttgatatatc ttcaaataaa
    22141 aaagctaagc cttgtatgta tgttggaact gaaaaatcaa cagtcttaaa tttaattgct
    22201 gataattcag gtcgcaatca atacaaagca attgtcgact acgtcgcaga tagtgcaaag
    22261 cagtttggga ttcgatatgc taatacgcaa acaaatgaag atatcgaaac acaggataag
    22321 ctgttagaat ttgcaaaaaa gcaaataaat gatactccta agactgaatt agatgttaat
    22381 tatataggtt atgaaaaaat agagccaaga gatagcgtat tttttgttca tgaattaatg
    22441 ggatataaca ctgaattaaa ggttgttaaa cttgataggt cacatccatt tgtaaacgca
    22501 atagatgaag tgtctttcag caatgaaata aaagatatgg tacaaattca acaagcactt
    22561 aacagacgag ttattgcaca agataataga tataactatc aagcaaatcg tataaatcat
    22621 ttatacacta gtactttgaa ttctcctttc gagacaatgg atatagggag tgtattaata
    22681 taatggcaac agaagaagtt aaaatcaaag cgctacttaa aaacgataaa cagtactttc
    22741 cagctacaca ctggaaagct ataaatggga taccttatgc aggcagtagt gatattgatg
    22801 gattgcctca agacggtatc atttcggtag atgataaaaa taaattagat aaattaaaaa
    22861 taggcgaagc aggaattatt caaaatagca ttgtacagaa atccccaaac ggtaaattgt
    22921 ggaaaataac agttgacgat agtgggaaac ttggtacagt gctattttat tagaaaggaa
    22981 ggtgcattat ggaaaatttg tatttaataa aggatttggg agctttagca ggtcgagatt
    23041 atagagctaa agaaattcaa aacctgcaaa gaatagagca atttgcgctt ggcttgacaa
    23101 cagagtttaa gttgcatcag aaagctaaaa caatgcaaca cttcgctgag caaatttatt
    23161 ataatggtag atcgcaagca gcagtaaaca aatctttaca aagtcaaatt aacgcacttg
    23221 ttgtggcacc acgtaataac agtgctaatg agattgttca agctcgagtt aatgtaaacg
    23281 gcgaaacctt tgacacatta aaagaacatt tagacgattg ggaaaccaaa actcaaatta
    23341 ataaagagga aactataaga gaattaaata agaccaaaca agaaattctt gatatcgagt
    23401 atcgttttga acctgataag caagagtttt tatttgtgac agaacttgca cctcttacaa
    23461 atgcagtaat gcaatccttc tggtttgata atagaacagg catagtatac atgacacaag
    23521 ctagaaataa tggctatatg ctaagtcgtt taagacctaa tggtcaattt atagacagct
    23581 cattgattgt aggtgggggt catggtacac ataacggtta tagatatatt gatgatgagt
    23641 tatggattta tagttttatc ttaaatggta ataatgagaa tacattagtt cgtttcaagt
    23701 atacgcctaa tgtggaaatt agctatggca agtatggtat gcaagatgta tttacaggac
    23761 acccagaaaa accctacatc acccctgtca taaatgaaaa agaaaataaa attctataca
    23821 gaattgagag acctagaagt cagtgggaac ttgaaaactc aatgaattat atagagataa
    23881 gaagtttaga cgatgttgat aaaaatattg ataaagtttt gcataaaatc agtatcccta
    23941 tgagactaac aaacgaaacc caaccaatgc agggtgtgac ttttgatgaa aaatacttgt
    24001 attggtatac aggagacagt aatccaaata atagaaacta tttaacggct ttcgatttag
    24061 aaacaggaga agaagcgtat caggttaatg ctgactatgg tggaacacta gattcatttc
    24121 ctggcgaatt tgcggaagca gaaggtttgc aaatatacta tgacaaagat agtggtaaaa
    24181 aagctttgat gctaggtgtt actgtcggtg gtgatggaaa tagaacacat cgtattttca
    24241 tgattgggca aagaggtatt ttagaaatac ttcactcaag aggcgttcct tttatcatga
    24301 gtgacacagg tggtagagtt aaacctttac caatgaggcc tgataaactt aagaatcttg
    24361 ggatgttaac agagccaggt ctttactatt tatacactga tcatacagtt caaatcgatg
    24421 atttcccatt accaagagaa tggcgtgatg caggttggtt cttggaagtt aagccaccac
    24481 aaactggcgg tgatgtaatt cagatattga cgcgtaatag ttatgcaagg aatatgatga
    24541 cttttgaaag ggtgctttct ggaagaactg gagacatttc ggactggaat tatgtgccta
    24601 aaaatagtgg taaatgggag agagtacctt cattcatcac aaaaatgtca gatattaaca
    24661 tagtaggcat gtcgttttat ttaactacgg atgatacaaa acgttttaca gattttccaa
    24721 ctgaacgtaa aggggtagct ggttggaact tatatgtaga agcttcaaac acaggtggct
    24781 ttgttcatag gctagttcgt aatagtgtta cagcatctgc tgagatacta ttgaaaaatt
    24841 atgatagtaa aacaagttca gggccatgga ctttacacga agggagaatt ataagttaat
    24901 gagtaattta gagaaatctg tagctataaa tttagaaaac acagcgcatt atgaaaatat
    24961 ttcaaatcta gatataactt ttagaacagg agagagtgat tcttctgttc ttctttttaa
    25021 tatcattaaa aataatcaac cgttattact gagtgaagaa aatatcaaag cacgaatagc
    25081 gattcgaggt aaaggagtaa tggtagttgc tccactagaa atattagatc catttaaagg
    25141 tattttaaaa tttcaattac ctaatgatgt aattaaaaga gatggaagtt atcaagctca
    25201 agtttcggtt gcagaattag gtaattcaga cgtggtagtt gtagagagaa ctatcacatt
    25261 taacgttgaa aaaagtttgt ttagcaagat tccctctgaa acaaaactac actatattgt
    25321 tgagtttcaa gaattagaaa aaactattat ggatcgcgcg aaagcaatgg acgaggctat
    25381 aaaaaatggt gaggattatg cgagtctgat tgaaaaagct aaagaaaaag gtctatcaga
    25441 tattcaaata gcaaaatctt caagtattga tgaattaaag caacttgcta atagccgtat
    25501 atctgatttg gaaaataaag cgcaagcata ttcaagaaca ttcgatgagc aaaagcgata
    25561 tatggatgag aaacatgaag ccttcaagca gtcagtgaat agtggtggtt tagtcacaag
    25621 tggttctact tcaaattggc aaaaagctaa gattactaaa gatgatggta agataatgca
    25681 gattactgga tttgatttta ataatccaga acaaagaata ggtgattcaa cccaatttat
    25741 ttatgtttcg caagctataa attatccaag agatgttagt actaacggta ctgtcgaata
    25801 tttagtagta acttcagatt acaagcgtat gacttatcga ccgaacggta caaataaagt
    25861 gtttgttaaa agaaaagaag cgggttcatg gtctgagtgg tcagaattag ctattaatga
    25921 ttacaataca ccttttgaaa ctgttcaaag tgcccaatca aaagctaata tggccgaaag
    25981 taacgctaaa ttatacgcag atgacaagtt taataaaagg tattcggtta tttttgatgg
    26041 aacagcaaat ggtgtgggct ctacattgta cttaaatgag agtttagacc aatttatttt
    26101 attaattttt tatgggactt ttccaggtgg tgactttaca gagtttggca gtccttttgg
    26161 aggaggaaag atttcattga atccctcaaa tcttccagat ggtgatggaa atggtggagg
    26221 tgtttatgag tttggattaa ctaaatctag tcgtacatct ttaactatat caaacgatgt
    26281 ctatttcgac ttaggaagtc aaagaggctc tggtgcgaac gcaaatagag ggacaattaa
    26341 caaaattata ggagtgagaa aataatgcaa atattagtta acaagcgtaa tgagataatt
    26401 tcatacgcta tcattggcgg ctttgaagaa ggtattgata ttgaaaattt accagaaaat
    26461 ttctctcaag tttttagacc taaagccttt aaatattcaa atggggaaat agtttttaac
    26521 gaagattatt cagaagaaaa agatgacttg catcaacaga ttgacagtga agaacaaaac
    26581 acagtcgctt ctgatgacat cttacgaaaa atggttgcta gtatgcagaa acaagttgtt
    26641 caaagtacaa agttatcgat gcaagttaat aagcaaaatg cactaatggc aaaacaactt
    26701 gtgacactta ataaaaaatt agaagaggtt aaaggagaga ctgaaaatgc ttaaattaat
    26761 ttcaccaaca ttcgaagata ttaaaacatg gtatcaattg aaagaatata gtaaagaaga
    26821 tatagcgtgg tatgtagata tggaagttat agataaagag gaatatgcaa ttattacagg
    26881 agaaaagtat ccagaaaatc tagagtcata ggttataatc ttatggcttt ttaatttgaa
    26941 taaagtgggt ggtgtaatgt ttggatttac caaacgacac gaacaagatt ggcgtttaac
    27001 gcgattagaa gaaaatgata agactatgtt tgaaaaattc gacagaatag aagacagtct
    27061 gagaacgcaa gaaaaaattt atgacaagtt agatagaaat ttcgaagaac taaggcgtga
    27121 caaagaagaa gatgaaaaaa ataaagagaa aaatgctaaa aatattagag acatcaagat
    27181 gtggattcta ggattaatag ggacgattct aagtacattt gttatagcct tgttaaaaac
    27241 tatttttggc atttaaagga ggtgatcacc atgcttaagg gaattttagg atatagcttt
    27301 tggtcgtgtt tctggtttgg taagtgtaag taatatttaa gagtcagtgc ttcggcactg
    27361 gctttttatt ttggataaaa ggagcaaaca aatggatgca aaagtaataa caagatacat
    27421 cgtattgatc ttagcattag taaatcaatt cttagcgaat aaaggtataa gtccgatacc
    27481 agtagatgaa gaaagtgttt catcgattat cttaacagtt gttgctttat atactacata
    27541 taaagataat ccaacatctc aagaagggaa atgggcgaat caaaaattaa agaaatataa
    27601 agctgaaagt aaatatagaa aagcaacagg gcaagcgcca attaaagaag taatgacacc
    27661 tacgaatatg aacgacacaa atgatttagg gtaggtgttg accaatgttg ataacaaaaa
    27721 accaagcaga aaaatggttt gataattcat tagggaagca gttcaatcct gatttgtttt
    27781 atggatttca gtgttatgat tacgccaata tgttctttat gttagcgaca ggcgaaaggc
    27841 tgcaaggttt atatgcttat aatatcccgt ttgataataa agcaaagatt gaaaaatatg
    27901 gtcaaataat taaaaactat gacagctttt taccgcaaaa gttggatatt gtcgttttcc
    27961 cgtcaaagta tggtggcgga gctggacacg ttgaaattgt tgagagcgca aatttaaaca
    28021 ctttcacatc atttggtcaa aactggaacg gtaaaggttg gactaatggc gttgcgcaac
    28081 ctggttgggg tcctgaaact gtgacaagac atgttcatta ttatgacaat ccaatgtatt
    28141 ttattaggtt aaacttccct aacaacttaa gcgttggcaa taaagctaaa ggtattatta
    28201 agcaagcgac tacaaaaaaa gaggcagtaa ttaaacctaa aaaaattatg cttgtagccg
    28261 gtcatggtta taacgatcct ggagcagtag gaaacggaac aaacgaacgc gattttatac
    28321 gtaaatatat aacgcctaat atcgctaagt atttaagaca tgcaggacat gaagttgcat
    28381 tatacggtgg ctcaagtcaa tcacaagata tgtatcaaga tactgcatac ggtgttaatg
    28441 taggcaataa aaaagattat ggcttatatt gggttaaatc acaggggtat gacattgttc
    28501 tagaaataca tttagacgca gcaggagaaa gcgcaagtgg tgggcatgtt attatctcaa
    28561 gtcaattcaa tgcagatact attgataaaa gtatacaaga tgttattaaa aataatttag
    28621 gacaaataag aggtgtaaca cctcgtaacg atttactaaa tgttaatgta tcagcagaaa
    28681 taaatatcaa ttatcgctta tctgaattag gttttattac taataaaaat gatatggatt
    28741 ggattaagaa aaattacgac ttgtactcta aactaatagc tggtgcgatt catggtaagc
    28801 caattggtgg agtggtagct agtgaggtca aagcgccagt taaaaacgaa aagaatccgc
    28861 cagtgccagc aggttataca ctcgataaga ataatgtccc ttataaaaaa gaacaaggca
    28921 attacacagt agctaatgtt aaaggtaata atgtaagaga cggttattca actaattcaa
    28981 gaattacagg ggtattaccc aacaacacaa caattacgta tgacggtgca tattgtatta
    29041 atggttatag atggattact tatattgcta atagtggaca acgtcgctat attgcgaccg
    29101 gagaggtaga catagcaggc aaccgaataa gcagttttgg taagtttagt gcagtttgat
    29161 aattgtatat gatgaatctt aggcaggtac ttcggtactt gcctattatt taaaattaat
    29221 aaacagttaa tttttacatg aatatattaa attttaaaaa aacaaacgtt tttagtatat
    29281 aaattatttt gtgttcgtat tgtgtgctat gattaaaaag ttgttatggt caactatatc
    29341 gtggttttat gtttattatc aatcaaaata taaattattt ataatttgtt tggtaatgaa
    29401 cgggtttttt tcgaaataat a
//
LOCUS       DAHHRR010000023        28044 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.023, whole genome shotgun sequence.
ACCESSION   DAHHRR010000023 DAHHRR010000000
VERSION     DAHHRR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 28044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28044
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.023"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            312..1571
                     /locus_tag="NBI42_000832"
     CDS             312..1571
                     /locus_tag="NBI42_000832"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCD1777269.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            1660..1995
                     /locus_tag="NBI42_000833"
     CDS             1660..1995
                     /locus_tag="NBI42_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1777270.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            2234..3661
                     /locus_tag="NBI42_000834"
     CDS             2234..3661
                     /locus_tag="NBI42_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCD1777271.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADNVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            3908..5224
                     /gene="rho"
                     /locus_tag="NBI42_000835"
     CDS             3908..5224
                     /gene="rho"
                     /locus_tag="NBI42_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCD1777272.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            5342..5596
                     /locus_tag="NBI42_000836"
     CDS             5342..5596
                     /locus_tag="NBI42_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCD1777273.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            5942..6541
                     /locus_tag="NBI42_000837"
     CDS             5942..6541
                     /locus_tag="NBI42_000837"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCD1777274.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            6542..7618
                     /gene="prfA"
                     /locus_tag="NBI42_000838"
     CDS             6542..7618
                     /gene="prfA"
                     /locus_tag="NBI42_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCD1777275.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESSGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            7605..8441
                     /gene="prmC"
                     /locus_tag="NBI42_000839"
     CDS             7605..8441
                     /gene="prmC"
                     /locus_tag="NBI42_000839"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCD1777276.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNIARNNAEKHQSQI
                     QFLTGDALKPLIKEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIQDINGHDRIVSF
                     KW"
     gene            8525..9571
                     /locus_tag="NBI42_000840"
     CDS             8525..9571
                     /locus_tag="NBI42_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCD1777277.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKPGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITDILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSDAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            9568..9987
                     /locus_tag="NBI42_000841"
     CDS             9568..9987
                     /locus_tag="NBI42_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCD1777278.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            10094..10618
                     /locus_tag="NBI42_000842"
     CDS             10094..10618
                     /locus_tag="NBI42_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCD1777279.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQFNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            10645..11883
                     /locus_tag="NBI42_000843"
     CDS             10645..11883
                     /locus_tag="NBI42_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCD1777280.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            11911..12540
                     /gene="upp"
                     /locus_tag="NBI42_000844"
     CDS             11911..12540
                     /gene="upp"
                     /locus_tag="NBI42_000844"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCD1777281.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            12564..13691
                     /gene="wecB"
                     /locus_tag="NBI42_000845"
     CDS             12564..13691
                     /gene="wecB"
                     /locus_tag="NBI42_000845"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCD1777282.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKNSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGQPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            13759..14211
                     /locus_tag="NBI42_000846"
     CDS             13759..14211
                     /locus_tag="NBI42_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCD1777283.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYLYLLIILGGLYLFTHHAFILGLIIGVTGSAINTVIFESYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYFNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            14232..14960
                     /gene="atpB"
                     /locus_tag="NBI42_000847"
     CDS             14232..14960
                     /gene="atpB"
                     /locus_tag="NBI42_000847"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCD1777284.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTQFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            15003..15215
                     /gene="atpE"
                     /locus_tag="NBI42_000848"
     CDS             15003..15215
                     /gene="atpE"
                     /locus_tag="NBI42_000848"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCD1777285.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            15413..15934
                     /locus_tag="NBI42_000849"
     CDS             15413..15934
                     /locus_tag="NBI42_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCD1777286.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            15934..16473
                     /locus_tag="NBI42_000850"
     CDS             15934..16473
                     /locus_tag="NBI42_000850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCD1777287.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLK
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            16495..18003
                     /locus_tag="NBI42_000851"
     CDS             16495..18003
                     /locus_tag="NBI42_000851"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCD1777288.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            18034..18900
                     /locus_tag="NBI42_000852"
     CDS             18034..18900
                     /locus_tag="NBI42_000852"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCD1777289.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTFRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            18922..20334
                     /gene="atpD"
                     /locus_tag="NBI42_000853"
     CDS             18922..20334
                     /gene="atpD"
                     /locus_tag="NBI42_000853"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCD1777290.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            20354..20758
                     /locus_tag="NBI42_000854"
     CDS             20354..20758
                     /locus_tag="NBI42_000854"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCD1777291.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            complement(21260..22237)
                     /gene="cobA"
                     /locus_tag="NBI42_000855"
     CDS             complement(21260..22237)
                     /gene="cobA"
                     /locus_tag="NBI42_000855"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCD1777292.1"
                     /translation="MSVDEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIVIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVGYTENTPKSYDPTKQFYVVSGSKHDALMLCEYLYDKG
                     YGCLLNPNDTSNGTYHPSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(22228..22542)
                     /gene="nirD"
                     /locus_tag="NBI42_000856"
     CDS             complement(22228..22542)
                     /gene="nirD"
                     /locus_tag="NBI42_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCD1777293.1"
                     /translation="METKEKIKVTTLDELTPLIGKKVIVKGKEIGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(22546..24951)
                     /locus_tag="NBI42_000857"
     CDS             complement(22546..24951)
                     /locus_tag="NBI42_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1777294.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEKMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAIKENGFITVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLNPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEV
                     EKV"
     gene            complement(24979..25746)
                     /locus_tag="NBI42_000858"
     CDS             complement(24979..25746)
                     /locus_tag="NBI42_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HCD1777295.1"
                     /translation="MNGNIIVAHGMRHGRQNQALLAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLVQSGVDHFKIVPLLIFSAMHYLKDIPDIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLRISKPT
                     YARTLYGEISFTHDLERISKRYQKLIVVPLFLYDGRLVNKVKQRMNNMVINPDIHITP
                     SINFDPILKQIINDRLESLNDSNENIKAQTKIKTFLK"
     gene            complement(25976..26512)
                     /locus_tag="NBI42_000859"
     CDS             complement(25976..26512)
                     /locus_tag="NBI42_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1777296.1"
                     /translation="MTNTTIRLANENDAETLQQLMYKAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRFPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(26925..27749)
                     /locus_tag="NBI42_000860"
     CDS             complement(26925..27749)
                     /locus_tag="NBI42_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCD1777297.1"
                     /translation="MIKNNKTVEDTYSTKAIVDSISSSVQMKQVMVQQTPGRYMLKSM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLVLVVLTNSELLTSNFMY
                     FTVGWYYKVVSINKMMWILLYCFLGNILGGFVLFFLMKYAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVIVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
BASE COUNT         9606 a         4568 c         5240 g         8630 t
ORIGIN      
        1 attggttgtg atataattat aaggaaaatg agcaatttat gaaaaaagtt tacgtgcaaa
       61 tcgtagaatt aaaactaaat aattgtcaaa acaacgtcaa tatttagttg aataatcaga
      121 ctttagacca tggtcaagtg gggaagaaca gcatatatta gtaaaggtga atgatttgtt
      181 attaatcaat cgaaaataga aagacaagat tttaacgatt aaaataaact attttacaaa
      241 taaagtaaaa ttaatttata atgctaataa tgcaaaaaat taaaaagtaa tggacaaagg
      301 agataaatga tatggctcaa gaggtaataa aaataagagg tggacgcaca ctaaatgggg
      361 aagttaatat cagtggcgcg aaaaacagtg ctgtagctat cattcctgca acattattag
      421 ctcaaggaca tgtgaaatta gaagggttac cgcaaatctc tgatgttaaa actttagtga
      481 gtttattaga agatttaaat attaaggctt cactaaatgg tacggaatta gaagttgata
      541 caactgaaat acaaaatgct gcattaccaa ataataaagt tgaatcatta cgtgcttcat
      601 actatatgat gggtgccatg ttaggtagat ttaaaaaatg tgtgattggt ttaccgggag
      661 gatgtccact tgggccacgt ccaattgatc aacatattaa aggatttaaa gctttaggtg
      721 ctgaaattga tgaatcaagt acgacatcaa tgaaaattga agctaaagag ttaaaaggtg
      781 cacatatttt tctagatatg gttagcgtag gtgcaacaat taacattatg ctagctgcag
      841 tttatgcaac aggacaaact gtaattgaaa atgctgctaa agaaccggaa gtagttgatg
      901 ttgctaactt cttaacaagt atgggagcta atattaaggg agcgggtaca tcaacgatta
      961 aaatcaatgg tgtcaaagaa ttacatggtt ctgagtatca agttatacct gatagaattg
     1021 aagcaggcac atatatgtgt atcgctgcag catgtggaga aaatgttata ttaaataata
     1081 ttgttccgaa acatgtagaa acattaactg ctaaatttag tgaattaggt gtgaatgttg
     1141 atgtaagaga cgaaagaatt cgcatcaata ataatgcacc atatcaattt gttgatatta
     1201 aaacactagt atatccaggt tttgctactg atttgcaaca gcctattaca ccattattat
     1261 ttatggcaaa tggtccttca tttgtaactg atacgattta tccggagcgt tttaaacatg
     1321 ttgaagaatt aaagcgcatg ggtgcaaata ttgaagttga cgaaggcaca gcaacgataa
     1381 aaccatcaac attacatggt gctgaagttt atgctagcga tttaagagca ggagcttgtt
     1441 taattattgc tggtttaatt gctgagggtg taactacaat ttacaatgtg aaacatattt
     1501 atagaggtta tacagatatt gtagaacact taaaagcttt aggtgcagat atttggacgg
     1561 aaactgtata gatttatctt gttttgtggt ataataatta tactgattaa aaaagggtgt
     1621 tcgtatacat tctaaattta ttgatgagag gtgaagatga tggaagtatg tccgtatctc
     1681 gaagaaactt ttaaaatact tggtagaagt tggaatggat taatcattaa ttatctctca
     1741 agatgtaatg actgttcagc acacttttcc gatatgaaaa gagatttgaa aacaataacg
     1801 ccacgtgctt taagtcttaa gttgtcagag cttgcacaat gggagttagt tgagaagcaa
     1861 atcatttcta cgagtccagt acaaattatt tatgtactaa ctgaaaaagg taaagcgtta
     1921 gcagaggctt tacatccaat tgaagcatgg gcgcaatcat atgtcgattt aacagatcaa
     1981 cgtactgcta aataattaca tgacgtttaa acatagcatt gattataact atttctaagt
     2041 cttcgcatta tttgcgatga tgtgggaata gttattttta tttaaaaata taaaaaaatt
     2101 agatgcagca aaattttaaa gcattttatt ttgaacatat taaaagggag cgtatcataa
     2161 tggaatgtaa tgtttatatc gtatgcatta cggataaata atatataaat cattcttgag
     2221 gagtgaaaga ataatgagag actacacaaa gcaatatatt aatggcgaat gggtagaaag
     2281 taatagtaat gaaacgatag aagttataaa tccagcaacc gaagaagtaa tcgggaaagt
     2341 tgctaaaggt aataaagctg atgttgataa agccgtcgag gcggcagaca atgtttattt
     2401 agagttccgt catacatctg tgaaagaaag acaagcatta ttagataaaa ttgtaaaaga
     2461 atatgaaaac agaaaagacg atattgtaca agctattacg gatgagttag gtgctccttt
     2521 atcattatct gagcgtgtcc attatcaaat gggactaaac cattttgttg cagcgagaga
     2581 cgcattagat aactacgaat ttgaagaacg ccgcggagat gatttagttg ttaaagaagc
     2641 tatcggtgta tctggcttaa ttacaccgtg gaacttccct acaaaccaaa catcattaaa
     2701 attagcagca gcatttgcgg ctggtagtcc agttgtactt aaaccatctg aagaaacacc
     2761 atttgcagct gttattttag ctgagatttt tgataaagtc ggtgttccta aaggtgtatt
     2821 taaccttgtt aatggtgatg gtgctggcgt tgggaatcct ttatctgaac atcctaaagt
     2881 acgcatgatg tcatttacag gatcaggccc tactggttct aaaattatgg aaaaagctgc
     2941 taaagacttt aaaaaggtat cattagagct tggtggcaaa tcaccataca tcgtcctaga
     3001 tgacgtagat attaaagaag cggctaaagc aacaacaggc aaagttgtta ataatactgg
     3061 tcaagtatgt acagctggta cacgtgtttt agtgcctaac aaaattaaag atgcattctt
     3121 agctgaatta aaagaacaat ttagccaagt gcgtgtcggt aatccaagag aagatggtac
     3181 acaagtaggc cctatcatta gtaaaaaaca atttgatcaa gtacaaaatt atattaataa
     3241 aggtattgaa gaaggtgctg aattatttta tggtggtcct ggtaaaccag aaggacttga
     3301 aaaaggatac tttgcacgtc cgacaatttt tattaatgta gataatcaaa tgacgatagc
     3361 acaagaagaa atttttgggc cagtaatgtc agttatcact tataacgatt tagatgaagc
     3421 gattcaaatt gcaaatgata caaaatatgg tttggcagga tatgttattg gtaaggacaa
     3481 agaaacattg cataaagtag ctcgttctat tgaagcaggt acagtagaaa taaacgaagc
     3541 aggtagaaag ccagacttac catttggtgg ctataaacaa tctggtttag gtcgtgaatg
     3601 gggcgattat ggtattgaag agttcttaga agtgaaatct atagctggct attttaaata
     3661 aatttgattt ttgaattaaa aatcgcaata aaacagtgca catgactaat taagttttgt
     3721 gtactgtttt aattttgcaa tttttataaa tagattttgt aattaaaata aaaatttgct
     3781 atagttattc atgtatttaa aaggttgggg attagcataa tgggattgtg ctagcacagt
     3841 tgtttatgca ttgtcatgcc tatctattac ttactaacta aaaaataatg aaatgggtgt
     3901 aaactatatg cctgaaagag aacgtacatc tcctcagtat gaatcattcc acgaattgta
     3961 caagaactat actaccaagg aactcactca aaaagctaaa actcttaagt tgacgaacta
     4021 tagtaaatta aataaaaaag aacttgttct agctattatg gaagcacaaa tggaaaaaga
     4081 tggtaactat tatatggaag gtatcttaga tgatatacaa ccagatggtt atggtttttt
     4141 aagaacagtg aactattcta aaggggaaaa agatatttat atatctgcta gccaaattcg
     4201 tcgttttgaa attaaacgtg gggataaagt aactgggaaa gttagaaaac ctaaagataa
     4261 cgaaaaatat tatggcttat tacaagttga ctttgttaat gaccataacg cagaagaagt
     4321 gaagaaacgt ccgcatttcc aagctttgac accactttat ccagatgagc gtattaaatt
     4381 agagacagaa acacaaaatt attcaacgcg catcatggat ttagtaacac cgattggttt
     4441 aggtcaacgt ggtttaatag tggcgccacc taaagcaggt aaaacatcgt tattaaaaga
     4501 gatagcgaat gcaatcagta cgaacaaacc agatgcaaag ctatttattt tgttagttgg
     4561 cgagcgtcct gaagaggtaa cagatttaga acgctcagta gaagctgctg aagtcgttca
     4621 ttcaacgttt gacgaaccac cagaacacca tgttaaagta gctgaattat tacttgaacg
     4681 tgcaaagcgt ttagtagaaa ttggggaaga tgtcattatt ttaatggatt ctataacgag
     4741 attagcacgc gcttataact tagttattcc accaagtggt cgtacattat caggtggttt
     4801 agatcctgca tctttacaca aaccaaaagc attcttcggt gcagcgagaa atattgaagc
     4861 aggtggaagt ttaacaatac ttgcaactgc attagttgat acgggttcac gtatggacga
     4921 tatgatttac gaagaattta aaggaacagg taacatggag ttacatttag atcgtaaatt
     4981 gtctgaacgt cgtatcttcc ctgcaattga tattggcaga agttcaacgc gtaaagaaga
     5041 attgttgata agtaaatctg aattagacac attatggcaa ttaagaaatc tattcactga
     5101 ctcaacagac tttactgaaa gatttattcg caaacttaaa aggtctaaga ataatgaaga
     5161 tttcttcaag cagctacaaa agtctgcaga agaaagtact aaaacgggtc gacctataat
     5221 ttaataaaca ttatataggg gcttgcgttt tgaattaatt acctttataa ttacacagta
     5281 ttgggtaaaa actcacaaat aactctgttc cagatggttc agggcaaagg agctgaaaat
     5341 aatgaaacaa ggaattcatc ctgagtatca ccaagtaatc tttttagata ctactacaaa
     5401 cttcaaattc ttaagcggtt ctacaaaaac atcttcagaa atgatggagt gggaagatgg
     5461 aaaagaatac ccagttattc gtttagatat ttcatctgat tcacatccat tctacactgg
     5521 acgtcaaaaa ttcgctgctg cggatggacg tgtggaacgt ttcaacaaaa agtttggtct
     5581 taaatcaaac aactaatata gtaatcgtta agctgtctgc atccaataat ggaattgtag
     5641 acagcttttt tatttataag cgtagtaagg ggatatactt acgactgtgt tcaaagaagt
     5701 gactcaatac catgttgtta atacataatg ataaattccc cctttattaa agtttgtatt
     5761 gagtagaaaa atcgcacgaa taagaaggaa caaagtagaa tagctgtata taaatatgca
     5821 agacaaattt ataaaataat tgttcatgta caatatcgat ataattatga aaaagacatt
     5881 tatgaatatt tgaaatatga tataataaag cgattatgtt ttgaaaaagg tggaactctc
     5941 aatgtatgaa acttaccatt ccggttggat agaatgtatt acaggtagta tgtttagcgg
     6001 taaatccgaa gaacttataa gacgtttaag aaggggcata tacgctaaac aaaaagtcgt
     6061 tgtatttaaa ccggcgattg atgatcgata tcataaagaa aaagtggtct ctcataatgg
     6121 taatgccatt gaagcgatta atatttctaa agcaagtgaa attatgacgc acgacttaac
     6181 taatgttgat gttataggaa tagacgaagt gcaatttttt gacgatgaaa ttgtaagtat
     6241 agtagaaaaa ctatcagcag atggacaccg tgttattgtt gctggattag atatggattt
     6301 taggggcgaa ccgttcgaac caatgcctaa attgatggct gtcagtgaac aagtaacgaa
     6361 acttcaagca gtttgtgctg tttgtggttc ttcatctagt agaacgcaac gattaataaa
     6421 tggtaaacca gctaaaatcg atgatccgat tatcttagta ggtgccaatg aaagttatga
     6481 gccacgttgt cgcgcgcacc atatcgtagc acctagtgat aataataagg aggaattata
     6541 agtgtttgat caattagata ttgtagaaga aagatacgaa cagttaaatg aactgttaag
     6601 tgacccagat gttgtaaatg attcagataa attacgtaaa tattctaaag agcaagctga
     6661 tttacaaaaa actgtagacg tttatcgtaa ctataaagct aaaaaagaag aattagctga
     6721 tattgaagaa atgttaagtg aaactgatga taaagaagaa gtagaaatgt taaaagagga
     6781 aagtagcggt attaaagctg aacttccaaa tcttgaagaa gagcttaaaa tattattgat
     6841 tcctaaagat cctaatgatg acaaagacgt tattgtagaa ataagagcag cagctggtgg
     6901 tgatgaggct gcgatttttg ctggtgattt aatgcgtatg tattcaaagt atgctgaatc
     6961 acaaggattc aaaactgaaa tagttgaagc gtctgaaagt gaccatggtg gttacaaaga
     7021 aattagtttc tcagtttctg gtaatggcgc gtatagtaaa ttgaaatttg aaaatggtgc
     7081 gcaccgcgtt caacgtgtgc ctgaaacaga atcaggtgga cgtattcata cttcaacagc
     7141 tacagtagca gttttaccag aagttgaaga tgtagaaatt gaaattagaa atgaagattt
     7201 aaaaatcgac acgtatcgtt caagtggtgc aggtggtcag cacgtaaaca caactgactc
     7261 tgcagtacgt attacccatt taccaactgg tgtcattgca acatcttctg agaagtctca
     7321 aattcaaaac cgtgaaaaag caatgaaagt gttaaaagca cgtttatacg atatgaaagt
     7381 tcaagaagaa caacaaaagt atgcgtcaca acgtaaatca gcagtcggta ctggtgatcg
     7441 ttcagaacgt attcgaactt ataattatcc acaaagccgt gtaacagacc accgtatagg
     7501 tctaacgctt caaaaattag ggcaaattat ggaaggccat ttagaagaaa ttatagatgc
     7561 actgacttta tcagagcaga cagataaatt gaaagaactt aataatggtg aattataaag
     7621 aaaagttaga tgaagcaatt catttaacac aacaaaaagg gtttgaacaa acacgagctg
     7681 aatggttaat gttagatgta tttcaatgga cgcgtacgga ctttgtagtc cacatgcatg
     7741 atgatatgcc gaaagcgatg attatgaagt tcgacttagc attacaacgt atgttattag
     7801 gagagcctat acagtatata gttggctttg cctcatttta tggtagaacg tttgatgtaa
     7861 actcaaattg tttgatacca agacctgaaa ctgaagaagt aatgttgcat ttcttacaac
     7921 agttagaaga tgatgcaaca atcgtagata tcggaacggg tagtggtgta cttgcaatta
     7981 ctttgaaatg tgaaaagccg gatttaaatg ttattgctac tgatatttca cttgaagcaa
     8041 tgaacatagc tcgtaataat gctgagaagc atcaatcaca aatacaattt ttaacagggg
     8101 atgcattaaa gcccttaatt aaagaaggta tcaagttgaa cggcttgata tctaatccac
     8161 catatataga tgaaaaagat atggttacga tgtctccaac ggttacgaga ttcgaaccac
     8221 atcaggcatt gtttgcagat aaccatggat atgctattta tgaatcaatc attgaagatt
     8281 tacctcacgt tatggaaaaa ggcagcccag ttgtttttga aattggttac aatcaaggtg
     8341 aggcacttaa atcaataatt ttaaataaat ttcctgacaa aaaaatcgac attattcaag
     8401 atataaatgg ccacgatcga atcgtctcat ttaaatggta attagaagtt atgcctttgc
     8461 tatgattagt taagtgcata gctttttact ttgtattatg ataaataaga aaggcgtgat
     8521 taagttggat actaaaattt gggatgttag agaatataat gaagatttac agcaatatcc
     8581 taaaattaat gaaataaaag acattgtttt aaatggtggt ttaataggtt taccaactga
     8641 aacagtttat ggacttgcag caaatgcgac agatgaagaa gctgtagcta aaatatatga
     8701 agctaaaggc cgtccatctg acaatccgct tattgttcat atacacagta aaggtcaatt
     8761 aaaagatttt acatatactt tggatccacg cgtagaaaag ttaatgcagg cattctggcc
     8821 gggccctatt tcgtttatat tgccgttaaa gccaggctat ctatgtcgaa aagtttctgg
     8881 aggtttatca tcagttgctg ttagaatgcc aagtcattct gtaggtagac aattattaca
     8941 aatcataaat gaacctctag ctgctccaag tgctaattta agtggtagac cttcaccaac
     9001 aactttcaat catgtatatc aagatttgaa tggccgtata gatggtatcg ttcaagctga
     9061 acaaagtgaa gaaggattag aaagtacggt tttagattgc acatcttttc cttataaaat
     9121 tgcaagacct ggttctataa cagcagcaat gattacagat atacttccga atagtatcgc
     9181 ccatgctgat tataatgata ctgaacagcc aattgcacca ggtatgaagt ataagcatta
     9241 ctcacccaat acaccactta caattattac agatattgag agcaaaattg gaaatgacgg
     9301 taaagattgg tcttctatag cttttattgt gccgagtaat aaggtggcgt ttataccaag
     9361 tgatgcgcaa tttattcaat tatgtcagga tgacaatgat gttaaacaag caagtcataa
     9421 tctttatgat gtgttacatt cacttgatga aaatgaaaat atttcagcgg cgtatatata
     9481 cggctttgag ctgaatgata atacagaagc aattatgaat cgcatgttaa aagctgcagg
     9541 taatcacatt attaaagggt gtgaactatg aagattctat tcgtttgtac aggtaacaca
     9601 tgtcgtagcc cattagcgga aagtattgca aaagaggtta tgccaaatca tcaatttgaa
     9661 tcaagaggta tattcgctgt gaacaatcaa ggtgtttcga attatgttga agacttagtt
     9721 gaagaacatc atttagctga aacgacctta tcgcaacaat ttactgaagc agatttgaaa
     9781 gcagatatta ttttgacgat gtcgtattcg cacaaagaat taatagaggc acactttggt
     9841 ttgcaaaatc atgttttcac attgcatgaa tatgtaaaag aagcaggaga agttatagat
     9901 ccatacggcg gaacaaaaga gatgtatgta catacctatg aagaacttgt aagtttaatt
     9961 ttaaaattaa aagatattat ttgctagtag gaattctgta taattagtat atgttaacag
    10021 ttaaataagt tataatagag gagtgtagca ctttgctata ttcctttttt atttttagga
    10081 agggtgggct tttatgaaag atttgacaat gttattagac gaattaaaag atatgtcctt
    10141 ttttaataaa ggagatatat gtttaattgg atgttcaaca tcggaagtca ttggcgaaaa
    10201 aatagggact gtcggttcga tggaagttgc agaaactatt tttaatgcat tagatgttgt
    10261 gagtaaagag acaggcgtta cttttgcttt tcaaggatgt gaacatatca acagagcgat
    10321 aacaatagaa aaatctcagt ttaatccatt aacaatggaa gaagtttccg tcgttccaga
    10381 tgtccatgct ggagggagtt tggcaacata cgcttttcag catatgaagg atccaatcgt
    10441 ggtcgaacat attactgttc cttgtggaat tgatattggt caaacgttaa taggcatgca
    10501 tattaaacat gtatgtgtac ctgtaaggac ttcagtaaag caagtaggac aggcgattgt
    10561 aactattgcc acatctaggc cgaaaaagat tggtggcgaa cgtgctaaat accaataagt
    10621 ttttttgaaa gaaggggaga ttttatgtct tatatcacca agcaagataa agttatcgca
    10681 gaagcaatcg agagagaatt tcagagacaa aatagcaaca tagagttaat cgcatcggaa
    10741 aattttgtat cggaagcggt tatggaagca caaggttcag tgttgactaa taagtatgct
    10801 gaaggctatc caggacgccg atattatggt ggctgtgagt ttgtagatgt tactgaaagc
    10861 atcgcaattg atcgtgctaa agcattgttt ggagctgaac atgtcaatgt tcaaccacat
    10921 tcaggttcac aagcgaacat ggctgtttac ttagttgcat tagaaatggg cgacacagtt
    10981 ttaggtatga atttgagtca tggtggtcac ttgacacatg gagcgcctgt taattttagt
    11041 ggtaaattct acaatttcgt tgaatatgga gtagataaag acacagaacg aatcaattat
    11101 gatgaagttc gtaaattagc gttagagcat aagcctaagc ttattgtggc aggagcatca
    11161 gcatattcaa gaacaattga cttcaaaaag tttaaagaaa tcgcagatga agtaaacgct
    11221 aagttaatgg tagacatggc acatattgca ggattagtag cggcaggttt acatccaaat
    11281 ccagtagaat atgctgattt tgtaacaact acaacacaca aaacattacg cggaccacgt
    11341 ggtggtatga ttttatgtaa ggaagaatat aaaaaagaca tagataaaac aattttccct
    11401 ggtattcaag gtggacctct tgagcatgtt attgcagcaa aagcagttgc ttttggagaa
    11461 gcgttagaaa ataatttcaa aacgtatcaa caacaagtgg ttaaaaacgc aaaagttctt
    11521 gcagaagcat taattaatga aggatttaga attgtttctg gtggtacaga taatcactta
    11581 gtagctgttg atgtaaaagg gtctatagga cttactggta aagaagctga agagacttta
    11641 gattcagttg gtatcacatg taacaaaaat accattccgt tcgatcaaga aaaacctttt
    11701 gtaacgagtg gtatacgttt gggtacacct gctgcaacaa cgcgtggatt tgatgaaaaa
    11761 gcttttgagg aagttgcaaa aatcatcagt ttagcattga aaaatagtaa agatgaagaa
    11821 aaattacaac aagctaaaga acgcgttgcg aaattaacag ctgaatatcc tctatatcaa
    11881 taaacataca caaagatagg agtaattttt atgagtaaag tacacgtttt cgatcatcca
    11941 ttaattcaac acaaactaag ctatattaga gatgtaaata caggtactaa agaatttaga
    12001 gaacttgtag acgaagttgg tatgttgatg gcatatgaag taactagaga cttagagctt
    12061 caggatgttg atattgaaac gcctgtaact aaaatgacgg ctaaacgatt agctggtaaa
    12121 aaattagcta tcgttccaat tttaagagct ggtttaggta tgactgatgg tattttaagt
    12181 ttagtacctg cagcaagagt tggacatatc ggtttgtatc gagatcccga aacattaaag
    12241 gctgtagagt attttgctaa gttacctcaa gatattactg aaagacaaat cattgttgtt
    12301 gatcctatgt tagcaacagg cgcatcagca attgaagcta ttacttcatt gaagaaacgt
    12361 ggcgctaaaa atattcgctt catgtgttta attgcagcac cagaaggcgt agaaaagatg
    12421 catgaagcac atccagatgt agatatttat attgctgcac ttgatgaaaa gctaaatgac
    12481 aaagcatata tcacaccagg gttaggcgac gctggagata gattgtttgg tactaaatag
    12541 aagaatcgga gataagtagt ttgatgaaaa agattatgac catatttgga acaagacccg
    12601 aagccataaa gatggctcct ttagttaaag ctttggaaca agaaaaaatg ctcgagccaa
    12661 ttgttgttgt tacagcacag catagagaaa tgcttgattc agtattaagt acctttgaaa
    12721 tcaaaccaaa atacgatttg aatattatga aatcaggaca gacactttct gagataactt
    12781 ctaaatcaat cactcaatta gaacaagtga ttcaattaga aaaaccagac atggtacttg
    12841 tacatggtga tacgatgacg acatttgcag gtggtttagc ggcattttat aatcaagtgc
    12901 cgattggaca cgttgaagca gggcttagaa gttatgataa atattcacct tttccggaag
    12961 aggtgaatag acaattagtt ggtgtattag ctgacttaca ttttgcacct actaaaaatg
    13021 ctgcatcgca tttgttaagt gaagggaaaa attcagaaag tgttgttgtc actggtaata
    13081 cagcgattga tgctatgaaa tatacagttg atgacaatta taaatctaac atcatggata
    13141 aataccatga caaaaaattc attcttatga cagcacaccg acgagaaaat atcgggcaac
    13201 ccatggaaaa tatatttaaa gcggttagac gcttaataga tgagtatact gatttagcac
    13261 tggtatatcc aatgcataaa aacccgaaag ttcgagaggt ggctcaaaag attttaggta
    13321 gtcatgacag aattgaattg attgaacctt tggacgtagt agatttccat aattttgcta
    13381 aaaaatctta ttttattttg acagattcag gtggaattca ggaagaagcg ccatcattta
    13441 ataagccagt tttagttttg cgaagcgtta cagaacgtcc tgaaggtgtt gaagctggca
    13501 cattaaaagt cattggaaca aataagcaaa atgtgtatca agctgcaaaa gaattaatcg
    13561 atgatgagag attatatcat caaatgtctg aagcatctaa tccttacggt gatggatttg
    13621 cttcagaaag aatagttaat catattaagt attatttgaa tttaatcact gaaaaaccaa
    13681 gcgatttctg agaaaaatag actttttgtc taattgcgtc taaattgtgt ctaactatgc
    13741 ttattttttt acaacaattt gaatagaatt gacaccacaa atcgcctata ttatcattta
    13801 gtttgtatga gtggaacggt tttcatgatt gatatcagtc acacggaggc atataaaatg
    13861 agtcgtttct acaacatttt taaacagttc attcaatatt atttgtattt attaataata
    13921 ttgggaggat tatacctttt tacacaccat gcatttatct taggattaat cattggtgtt
    13981 actggttctg caatcaatac tgttatattt gagagctact tagccaaagc taaaagacca
    14041 gacactatgc atatttcaac tggaaatatg tggcgatact tagttgcaat tattgcctgt
    14101 atgatttggt actttaataa agcgcatgta agtataatcg gtataattat tggtttaatg
    14161 atttcatatg ttgtagttat catacgtcct ttactaaagg tgagcaaata aattaagaaa
    14221 gaggtgagat tatggatcac aaatccccgc tcgtgagttg gaatttattc ggttttgata
    14281 tcgttttcaa tttatcaagt atattgatga tacttgttac ggcgtttctt gtttttctac
    14341 ttgctatcat ttgtacgcgt aatttgaaaa aaagaccaac tggcaaacaa aatttcgttg
    14401 aatggatttt tgatttcgtg aggggaatca ttgaaggtaa catggcttgg aaaaaaggtg
    14461 gtcaattcca cttcttagca gtaacgctga ttctgtacat ttttatagct aatatgttag
    14521 gtcttccgtt ttctatagta acgaaagatc acacattgtg gtggaaatca ccaacagctg
    14581 atgcaacagt gactttaacg ttgtctacaa cgataatact gttaactcaa ttttatggaa
    14641 ttaaaatgcg tggtacgaaa caatatctta aaggttatgt acagccgttt tggccattgg
    14701 caattattaa tgtttttgaa gagttcactt caacattaac gctcggtctg cgtttgtacg
    14761 gtaacatatt tgcaggtgag atactattaa cattacttgc tggcttattc tttaacgaac
    14821 cagcatgggg ttggattatt agtatcccag gattaattgt ttggcaagca ttttcaatat
    14881 ttgtaggaac aatccaagca tatatcttta ttatgctttc gatggtttat atgtcacata
    14941 aagtggcaga tgaacactaa aaatttcaat aattatatac aatcacagga ggaaattaaa
    15001 ttatgaattt aatcgcagca gcaatcgcaa ttggtttatc agcattagga gcaggtatcg
    15061 gtaacggttt aatcgtttca agaacagttg aaggtgtagc acgtcaacca gaagcacgtg
    15121 gtcaattaat gggtatcatg ttcattggtg taggtttagt tgaggcatta cctatcatcg
    15181 gtgtagtaat tgcattcatg acatttgctg gataattaac agataaaaga ggtcgggaca
    15241 aagcgcatag gacataattc atgatgcata tatagtaata tctttgaact ttattaaata
    15301 gttgagatat gaatgcacca tgcctatcgc ataaattcag taggtcctaa cctcgtcgtt
    15361 tttttctata taacactagc gattatttta atgaaaggag tgtcatgaac ccgtgactga
    15421 aacagctaac ttattcgttc ttggtgcagc tggaggcgtt gagtggggta ctgtgattgt
    15481 acaggtccta actttcatcg tgttacttgc gttacttaaa aagttcgcat ggggtccatt
    15541 gaaagatgta atggataaac gtgaaagaga tattaacaga gatatcgatg acgcagaaca
    15601 agctaagtta aatgcacaga aacttgaaga agaaaataaa caaaaactta aagaaacaca
    15661 agaagaagtt caaaagattt tagaagatgc taaggttcaa gcacgtcaac agcaagaaca
    15721 aattattcat gaagcaaacg tacgtgcaaa cggtatgatt gaaacagcac aaagtgaaat
    15781 caatagccaa aaagaacgtg ccattgcaga tattaataat caagtatctg aactatcagt
    15841 gttaattgct tctaaagttc ttagaaaaga aatttctgaa caagaccaaa aagcattggt
    15901 tgacaagtat ctaaaagagg caggcgataa ataatggtaa aagtagctaa caagtatgct
    15961 aaagcattat ttgacgtgtc attagataca aataatttag aaactattaa tgaagaatta
    16021 acagttataa atgaagcagt aaaagataaa attgagcaat tgaaaatggt tgacagtaat
    16081 ccaactcaaa cagcagaaca acgtcgtgag ttgattaatg gtgtatttac tgacattaat
    16141 ccatacatta aaaatatgat gtacgtgtta gcagataata gacatatctc attaatagct
    16201 gacgtattca aggcgttcca aagcttatat aacggacact acaatcaaga ttttgcaaca
    16261 attgagtcaa catatgaatt gagtcaagaa gagttagata agattgtcaa actagtaact
    16321 caacaaacga agttatctaa agttattgta gatacaaaaa ttaatccaga tttaattggt
    16381 ggatttagag ttaaagtcgg cacaactgta ttagatggta gtgttagaaa tgatcttgtc
    16441 caattacaaa gaaaatttag aagagttaat taattataaa gaggagtgac atagatggcc
    16501 ataaaagctg aagaaatcag tgcattactt cgctcacaaa ttgaaaatta tgagtcagaa
    16561 atgtccgtaa ctgatgtagg tactgtatta caaattggtg atggtattgc attaattcac
    16621 ggattaaatg acgttatggc tggtgagcta gtagaattcc ataacggcgt acttggttta
    16681 gcccaaaacc ttgaagagtc aaacgtgggt gtggttattt taggaccata cacaggtatt
    16741 actgaaggtg acgaagttaa acgtactggt cgtatcatgg aagtaccagt aggtgaagaa
    16801 ctaatcggaa gagttgttaa tccattagga caacctattg atggacaagg accgattaac
    16861 acaactaaaa cacgtccagt agagaaaaaa gctactggtg taatggatcg taaatcagta
    16921 gatgagccat tacaaacagg tatcaaagca attgatgctt tagtaccaat tggtagaggt
    16981 caacgtgagt taatcatcgg tgaccgtcaa acaggtaaaa caacaattgc aattgacaca
    17041 attttgaacc aaaaagatca aggtacgatt tgtatctatg ttgctattgg tcaaaaagat
    17101 tcaacagtaa gagcaaatgt tgaaaagtta agacaagcag gcgctttaga ctacactatt
    17161 gttgtagcag catcagcttc tgaaccttct ccattattat atattgcacc atattcaggt
    17221 gtaacaatgg gtgaagaatt catgtttaac ggtaaacatg ttttaatcgt ttatgatgat
    17281 ttaactaaac aagcagcagc ttatcgtgag ttatcattat tattacgtag acctccaggt
    17341 cgtgaagcat acccaggtga cgtattctac ttacatagta gattattaga aagagcagca
    17401 aaattaaacg atgacttagg tggcggttca attactgcat taccaattat cgaaacacaa
    17461 gctggtgata tttcagctta tgtaccaaca aacgttattt caattacaga tggacaaatc
    17521 ttcttacaat ctgatttatt cttctcaggt gtaagaccag cgattaatgc cggacaatct
    17581 gtatctcgtg ttggtggatc tgcacaaatt aaagcaatga agaaagttgc tggtacgtta
    17641 cgtcttgact tagcgtcata cagagaactt gaatcatttg cacaattcgg ttcagacctt
    17701 gatgaattta ctgcaagtaa attagaacgt ggtaaacgta ctgttgaagt cttaaaacaa
    17761 gatcaaaaca aaccattacc agttgaacac caagtgttga ttatttatgc attaacaaaa
    17821 ggatatttag atgatattcc agttgtagat atcacacgtt ttgaagacga gttaaaccac
    17881 tgggcagaat caaatgctac tgaactgtta aatgaaatca gagaaactgg tggcttacca
    17941 gatgctgaga agtttgacac agcaattaac gaattcaaaa aaagctttag caaatctgaa
    18001 taataaacaa gtttagtata aggtggtgag atagtggctt ctcttaaaga aatagatact
    18061 cgaataaaat caaccaaaaa aatgaagcag attacgaaag cgatgaacat ggtatcaagt
    18121 tcaaaacttc gtagagctga aaaaaataca aaacaattca caccatatat ggataaaatg
    18181 caagatgcaa ttactgcagt tgcaggggca agtagcaata caaaccatcc aatgctaaga
    18241 cctcgcaaaa ttactagaag tggatattta gttatcacga gtgataaagg tttagcaggt
    18301 gcatatagtg caaacgtgct taaaaaattg attactgata ttgaagcgaa acatcaagat
    18361 agtagcgaat acagtattgt agttttaggg caacaaggtg ttgatttcct aaaaaataga
    18421 ggttatgaca ttgagtattc tcaagtagac gtacctgatc aaccttcttt caaatctgtt
    18481 caagcactag ctaaccatgc tatagactta tacagtgaag aagaaattga tgaattaaat
    18541 atatactata gtcattatgt cagcgttctt gaaaacaagc ctacatttag acaagtatta
    18601 ccattatctc aagaggattc tagtaagggg catggtcatt tgtcttctta tgaatttgag
    18661 ccagataaag aatctatctt aagtgtaatc ttgcctcaat atgttgagag tttgatttac
    18721 ggaacaatat tagacgcaaa agcaagtgag catgcaacac gtatgactgc gatgaaaaat
    18781 gccactgata atgcaactga acttattgat gacttatcat tagaatataa cagagcgaga
    18841 caagcagaaa ttacgcaaca aattactgaa attgttggtg gttccgcagc gcttgaataa
    18901 tatttaaagg aggaaaatag catgggaatt ggccgtgtaa ctcaagttat gggtcctgta
    18961 attgatgttc gatttgaaca taacgaagtt cctaaaatta ataacgcctt ggttattgat
    19021 gtgcctaaag aagaaggtac aatacaacta acattagaag ttgcgctgca attaggtgac
    19081 gacgttgttc gtacaattgc gatggattca actgatggtg tccaaagagg catggatgta
    19141 aaagatacag gcaaagaaat tagtgtacct gttggtgacg aaacattagg tcgtgtattt
    19201 aatgtactag gtgaaacaat tgaccttaaa gaagaaatta gtgattctgt tcgccgcgat
    19261 cctatccatc gtcaagcacc agcattcgat gaactttcaa cagaagttca aattttagaa
    19321 acaggtatta aagtagtaga tttactagca ccttatatta aaggtggtaa aatcggatta
    19381 ttcggtggtg ccggtgtagg taaaacagta ttaatccaag aattaattaa caacatcgct
    19441 caagagcacg gtggtatttc tgtattcgcc ggtgtaggtg aacgtactcg tgaaggtaac
    19501 gatttatact tcgaaatgag tgacagtggt gtaattaaga aaacagccat ggtattcggg
    19561 caaatgaatg agccacctgg tgcacgtatg cgtgttgcat tatctggttt aacaatggct
    19621 gaatatttcc gtgacgaaca aggtcaagac gtattattat tcatcgataa cattttcaga
    19681 tttacacaag ctggttctga ggtatctgca ttattaggtc gtatgccttc tgcagtaggt
    19741 taccaaccaa cacttgctac tgaaatggga caattacaag aacgtattac gtctacaact
    19801 aaaggatcag ttacttctat tcaagcggta ttcgtacctg ccgatgacta tactgaccca
    19861 gcgccagcga cagcgtttgc ccatttagat gcaactacaa acttagaacg taaattaact
    19921 gaaatgggta tttatccagc cgtggatcca ttagcgtcta catcaagagc attggaacca
    19981 tcaattgtag gtcaagaaca ttatgaagta gctcgtgatg tacaatcaac acttcaaaaa
    20041 taccgtgaat tacaagacat cattgctatc ttaggtatgg acgaattatc tgatgaagat
    20101 aaacaaacag ttgaacgcgc acgtagaatt caattcttct tatctcaaaa cttccacgta
    20161 gcggaacaat ttactggtca aaaaggttct tatgtacctg ttaagacaac agttgcaaac
    20221 tttaaagata tcttagatgg taaatatgac catattccag aagatgcatt ccgtttagtt
    20281 ggtagcatgg atgatgttat tgcaaaagct aaagatatgg gtgttgaagt ataacaatta
    20341 ggaggaatgg ataatgaata cattaaacct agatattgtc actcctaatg gttctgttta
    20401 caatcgtgat aatgttgaac tcgttgttat gcaaacaaca gctggtgaga taggtgtcat
    20461 gagtggacat attccaactg tagctgcttt aaaaacaggc tttgtaaaag tgaaatttca
    20521 tgatggaact gaatatattg ctgtaagcga tggctttgtt gaagttagaa aagataaagt
    20581 ttcaatcatt gttcagactg cagaaactgc aagagaaatt gatgttgaaa gagctaaatt
    20641 agccaaagca agagcagagt ctcacttgga aaatgatgac gacaatactg atattcatag
    20701 agccgaaaga gctttagaga gagcaaataa ccgtttgcgt gtggctgaat taaaatagta
    20761 aataaagggt cgaagatgtg atttcatatc ttcgaccctt ttttgaatta tattgattta
    20821 aagatacaaa acatgagagg gggaaggaat tgataaagaa ccattaaaga tttatgatgt
    20881 agtggttctt tatcattaaa cacagctaat gtgtatttaa aaataggagg gagtgggaca
    20941 gaaatgatat ttttacaaaa tttatttcgt tgtcccaccc caacttgcat tgtctgtaga
    21001 aattgggaat ccaatttctc tttgttgggg ccccgccggc aaggttgact agagttgaaa
    21061 aaagcttgtt gcaagcgcat tttcattcag tcaactactg ccaatataat attatagacc
    21121 ctaggacatt gatttatgtc ccaagctcta tacttattgc caacctaaat atttcaaatt
    21181 tggcacaacg acaaataagg cttcaacaca aatatattct ctcggttgaa accttactta
    21241 ttcatttatt ttttataaat tagtgacata acactgtatt agcatctgca cgatcggttg
    21301 aaatatatgt tacattttct tgctgcttaa taaatgcatc atagtaatca tattgcgacg
    21361 gatgatatgt gccattcgat gtatcatttg ggtttagcaa acaaccataa cctttgtcat
    21421 ataaatattc acagagcata agggcgtcat gtttagaacc acttactaca taaaattgct
    21481 tcgtaggatc atatgattta ggagtgtttt cagtatagcc aacaacttcc ccaataatac
    21541 atatacctgg tttcgcctca attgaatagt gttgcaattt tgaaataata ttacttaaac
    21601 gccccttaac aacaaactcg ttaaaacacg atgcttgaaa gacaatcact atcgggtaat
    21661 caatatctgt gtactgttgt atctgtgtga taattttccc taaacgtttt actcccatat
    21721 aaattgctaa cgtgccacca ttcactaagg aattgacatc cacttcattt tcttctgaat
    21781 ctttaaagtg acctgtagaa aatgtcacac ttttagcaac tgtacgcatt gtcaaacctg
    21841 tctgcatagt agcaactgct gcgctcgctg atgtcacccc tggtacaatt tcaaacgcaa
    21901 tatgatgttc atttagtgtg tcgacttctt cttgcacacg accaaatatc gctggatcgc
    21961 cacctttaag tctaacaacc ttgttataac gacgcgctgc ttccacgata cagtcattta
    22021 ttttttcttg ctgaatatgt tttgcatacg gctttttacc aacatcgata atttcagtag
    22081 tcaaattcgc atattgtaaa atcaacggat tcactaatcg atcatataga atgacatccg
    22141 cttcacgtat taaacgctca gcttttttag tcaaataatt cggattacct ggtcccgcac
    22201 ctatcaagta aaccttgcca tattcatcta cagacatata tatacgttcc cgtctgtaac
    22261 ttctacctca taaacatcta cacaaccttc atcaggttct tgaacaatac ctgtatttaa
    22321 atcaattttt tgatcgtgga gcgggcaaaa tacatattcc ccactcactg tcccttcaga
    22381 caatggtcct tgtttgtgtg gacagatatt gtgaatcgca tgaattttgc cactttctgt
    22441 taaaaacaat cctatttctt tgccttttac aataaccttt ttaccaatta agggtgtcaa
    22501 ttcatctaaa gttgtcactt tgattttctc ttttgtttcc atgtattaca ccttctccac
    22561 ttcaaaaatt ttacgtgctt gcgcattgct agttattgct tcccaaggtt cagcttcgac
    22621 tgctttttta gcatccataa tgcgttcaaa tagttcattt tgtctttctg ggttaagtaa
    22681 gacttctttt acattttcaa atccaagtct tcttaaccat ggcgctgttc tttcagcata
    22741 tatacctgtt tcgcgataat attgcatcaa agcaccacat aatttgatta cttcatcttc
    22801 tgtttctaca gttgttaaaa attcagcttt ttcaacttct gtacctccat taccaccgat
    22861 atagatttgg aacccatttt caactgagat aataccaaaa tctttaacac ctgattcaac
    22921 acaacttctt gggcagcctg atacacccat tttgaattta tgaggtgtat cgatgtattc
    22981 aaatgttttt tctaaacgaa tgccaagtcg tgtcgtgtat tgcgtaccaa atcgacaaaa
    23041 ctctttacca acacagcttt taactgagcg tgttttctta ccataagctg atgctgaacg
    23101 catacctagg tcttcccata tatttggtaa ttcttctttt ttaactccat acaaaccaac
    23161 acgttgtgaa cctgtcactt taactaatgg tacatgatat ttcttagcca cttctcctag
    23221 acgaatcagt tggtctgcat ctgtaacacc tccacgcatt tgaggtataa cagaaaatgt
    23281 accatcattt tgaatattcg catggtaacg ttcgttagca aatcttgatt ctctttcatc
    23341 ttcatgatca tgtggataaa ccatgtttaa ataatagttg attgctggtc gacattttgg
    23401 acatccacct ttatttttaa agtttaaaac atgtcgaact tctttagatg tttttaaacc
    23461 tttcgctctt atttgcgtta caatttgatc gcgtgtcaaa tcagtacaac cgcatatacc
    23521 agcaggtttt gcggcaacaa agtcatctcc taaagtgtgc tgcaatattt gagcaatttg
    23581 cggtttacat ttaccacatg aattccccgc ttttgtttta gccgttactt cttcaactgt
    23641 tataaagcca ttttccttaa tcgcatttac tatagtgcct ttatcaacac cattacaacc
    23701 acaaattgtt tcatcatcag ccatatcagc aattgatagc gatgcctctt ctccaccttt
    23761 agtaagcaat gataccagtg tgtaatcttc agtagattca ccttttttca tcatgttata
    23821 aaagcgtgaa ccatcatcga tatcaccata tagtactgca ccaactacat taccgtcttt
    23881 taaaaagatt tttttatagt tattatcaac gctattaaat atttcaatac ctttaatttc
    23941 tgcattttct acaatttgac cagcgctata caagtcacac ccagaaactt ttaatgacgt
    24001 aaatgttgtt gatcccttgt atccgttcgt ttctttattt gttaaatgat cagctaatac
    24061 tttaccttgt tcatatagtg gtgcaacgag tccataaact ttgccgttat gttctgcaca
    24121 ttcaccaact gcatatacat tgctatcact tgtttgcatc acatcattga caacaatacc
    24181 acgattaaca tctagacctg attctttggc tacttctgtg tatggtcgaa tacctactgc
    24241 cataacaact aagtctgccg gaatctcgcg tccatcagcc aatttaacac cttcaacatc
    24301 atcttctcct aagatttcag ttgtgttggc ttgcatttca aacttcatac cttgcttttc
    24361 tagatctgct ttaagcatat ttccagcttt acggtctagc tgcatttcca tcaaccattc
    24421 agctaaatgt aacaccgtta cttccatacc ttgatctaat aaaccacgtg cacactctaa
    24481 acctagtaag cctccaccaa ttacaattgc tttcttttta gtcttagcaa tgttcatcat
    24541 tttttcagta tcatcaatcg ttctccaacc aataacactt ggtaatgtcg aacctggaac
    24601 aggaatgacg aatgctttag aacctgtcgc aaaaatcaat ttatcgtatg atacttcaat
    24661 accatttgca gtagtaactg attgatttgc tctatctact tcaatcacag gatcatttgt
    24721 aattaactcg ataccatgtt cctcatacca ctcataggga ttcataattg tttcttcaac
    24781 tgtcatttta ttttgtaaaa tatttgaaag catgatgcgg ttatagtttg gataaggttc
    24841 tttaccaatt accgtaatat catataaatc gttggcgcgc tctaatattt cttcgattgt
    24901 tcgaatgcct gccataccgt taccaatcat tactagtttt tgctttgcca taaaatatgc
    24961 ccctttactt cataatattt atttcaaaaa ggttttaatt ttcgtttgtg cttttatatt
    25021 ttcattggag tcattaagac tttctaatct atcgttaata atttgtttta aaatcgggtc
    25081 gaagttaatt gaaggtgtga tgtgtatatc tggattaata accatgttat tcattcgctg
    25141 cttcactttg ttaacaagtc ttccgtcata taaaaataaa ggtacgacaa tcaatttttg
    25201 ataccgtttc gagattcttt ctaaatcatg tgtaaaacta atctctccat atagcgttct
    25261 cgcatatgtc ggcttgctaa ttcgcaaatt ttgagcgcat atttgtaact cttcgtgtgc
    25321 cttagtaaac tttccattaa tattgccgtg tgcaacaacc ataactccaa cttgttgttc
    25381 gtcacctgct aatgcatcac aaatacgttg ttcaattaat cgtctcatta aaggatgtgt
    25441 gccaagtggc tcgcttactt ctacctttat gtctggatac cgtcgtttca tttcatgaac
    25501 gatatccggt atatccttga gataatgcat tgcactaaag attagcaatg gtacaatttt
    25561 aaaatggtca accccacttt gaaccaacgt cgtcattacc gtctctaaat cctgatgctc
    25621 actttctaaa aacgcaatat catagtgatg tatatcatct tttactaatt cagaaataaa
    25681 tgctaataac gcttgattct gtcgtccgtg cctcatgcca tgtgcaacaa tgatattccc
    25741 attcacattt accaaccctt tcacacgtat tgtataccaa atcatttcgt ttttgtgaaa
    25801 agaatcacat tataatgtaa aatcagggaa ttccctgatg cctgtagtca tgcatattcc
    25861 ttatacattt tccctttttg ttaaatcaaa aaaaacgacc gatatatgaa tccctactca
    25921 acattcattt gagcaagcat caatatatcg gtcgcttgta gtgtatatta ttatcttaaa
    25981 atggtggttg gcctaatatt gtttcgtcaa agcgctcagg tatcaatact ttgcgcataa
    26041 tcacacctaa atcgccatca tcattttcat gttcgctgta tatttcataa cctctttttt
    26101 cataaatttt aagtaaccac ggatgcaatc ttgcagatgt acctaaagta actgccgctg
    26161 actttaacgt atctcgcaaa aatgcttctt caacataagt aagtaattgg ctaccatagc
    26221 ctttcccttc atactcagga tttgtcgcaa accaccagac aaaaggatag cccgaaatgc
    26281 ttttcacact tccccaagga aatctaaccg taatcgtaga tataatttca tcatcaattg
    26341 tcatgacaaa tgtagtattt ttatctatat tttctttaac agcatctaaa ttagcattaa
    26401 ccgaaggcca gtcaattcca agttctctta aaggtgtgaa tgccttatac attaactgtt
    26461 gtaacgtttc tgcatcgttt tcattcgcca gtctaatcgt agtgtttgtc atttcaatcc
    26521 ccatcacttt ctttttattt ggaatgcatc atagttcatt caaactaaaa atccccttat
    26581 agcgtaccaa catttagcac atgtgtcaga taacatgcct catctaaata agataggcat
    26641 gcgataatcg gttatttcat tattgactgc atcagtaaaa aagtctagga catgaattaa
    26701 tcatatgcac ccttcaaagc tttcattttt caatgtctac tttgaagcgt acagttcata
    26761 atgtcctaga ctctacaatg ttatattggc agtagctgac tgcgggcccc aacacagaag
    26821 ctggcggaaa gccagcttac aataatgtgc aagttgggga acggggccca aaaaaggatg
    26881 attctaaatt cattgttgtc acaacatctt caattatgac tccattactg ttgctctaat
    26941 cgattgcgtt taccatttaa aaatgcataa acaagtccta caaaaatacc gccacctaca
    27001 aagttaccaa ggaatgcaaa aacgatattt tttaatacat gtaaccatga aactgcatca
    27061 aggttaaaga ataccatacc tgcatataaa ccagcattga atacaacgtg ctcatatccc
    27121 atgtatacaa agactataac accacaagca atgaagaatg cttttgtcaa tccgcctttg
    27181 aattgcatag aaataaaaat accgatatta ataaagaagt tacagaaaat accttttgta
    27241 aaaatattca accaagtcga atcgactgtc tttttctgaa ctaaggcagt taaggcttgt
    27301 gtcatttctg gtgtcataac atgtgcatat ttcattaaga agaataatac aaaaccacct
    27361 aaaatatttc caaggaaaca atataataaa atccacatca tcttattaat agaaacaact
    27421 ttataatacc agccaactgt aaaatacata aaattacttg ttaataattc agaattcgtt
    27481 agtacaacta agaccaatcc taagctgaaa gcaattgctc ccattaaatt aatgagacca
    27541 tcaacatgtg tacttgcgaa ttgtgtttta ataccaaaca taaaaacagt aacaatggac
    27601 aataagaaac ctgccatcat agatttaagc atgtatcgtc ctggtgtttg ctgtaccatc
    27661 acttgtttca tttgaacaga tgatgatatt gagtctacga ttgctttagt tgaatacgta
    27721 tcttcaactg ttttattatt ttttatcaca gcacttcccc catttactat tatcaaataa
    27781 attttaccta ttaattatat tagtatttag atgtaattca tagattaaaa ttcagaaaat
    27841 tctttttacc aattcaaaac taatatttac tcataaaaat agcgagatag atatcgcgac
    27901 ataaaacaat tggtatcatt ttaattgcgc tcatctcgct ttatgtaagt attacttatt
    27961 cttttttaaa tttgaaaagt aatgtaaacc atatgcaaat ataccgtagc ctacgactgt
    28021 acgcaaaata aatctaacaa cttt
//
LOCUS       DAHHRR010000024        27902 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.024, whole genome shotgun sequence.
ACCESSION   DAHHRR010000024 DAHHRR010000000
VERSION     DAHHRR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27902
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.024"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..769
                     /locus_tag="NBI42_000861"
     CDS             <1..769
                     /locus_tag="NBI42_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCD1777298.1"
                     /translation="LILITLLFGYSCYLLLEAKAETQNDPNISELNKASQYTGSWHNI
                     WYLYNSDPVKAKKTKLSDKFLSHDFIVPINSPGHYDYVKTELKDSTMASSFDGKEVDI
                     FGVNYFHQCYFSNENIQCDSNQGAGSKKTCMYGGITLNENNTNNRIQPIVVKVYENDS
                     VTLSFDINIDKETVTIQELDYKVRNKLISKINLYHLGGTSYETGYIKFIENANRYYWY
                     DMMPDPSFTQSKYLMIYRGNETVESAKTEIEVHLTKK"
     gene            complement(954..1940)
                     /locus_tag="NBI42_000862"
     CDS             complement(954..1940)
                     /locus_tag="NBI42_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCD1777299.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKLEL"
     gene            2249..2452
                     /locus_tag="NBI42_000863"
     CDS             2249..2452
                     /locus_tag="NBI42_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777300.1"
                     /translation="MEDLKQSLKGLGWYDLFFIVPMFLLFVYLPNYNFITIFINIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            2501..2797
                     /locus_tag="NBI42_000864"
     CDS             2501..2797
                     /locus_tag="NBI42_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCD1777301.1"
                     /translation="MATEKDINDLFLNHVNSNAVKTRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSARHQFQDNTLVSSYPGAKEMILIPDITEATNLTNLFECIKNDLKK"
     gene            complement(2860..4080)
                     /locus_tag="NBI42_000865"
     CDS             complement(2860..4080)
                     /locus_tag="NBI42_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1777302.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YEINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSFIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTDKIKYAHKEE"
     gene            complement(4073..4624)
                     /locus_tag="NBI42_000866"
     CDS             complement(4073..4624)
                     /locus_tag="NBI42_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCD1777303.1"
                     /translation="MITCLFGSSRKDGNSAIAVENLLKNLNVNFVNLYQSNIEKVTDN
                     RHSKGANFHNDDYEQILIKVLESDIIIFSTPLYWYSMSASLKLFIDRWTESLRDTQID
                     NFKEIMSQKKYLILIIGGDNPHTKAQPLVNQFKLIFEFMNITNYRFLIGEGNKPYDIL
                     NDSQFIKELANINLALKKGDIYD"
     gene            4737..5597
                     /locus_tag="NBI42_000867"
     CDS             4737..5597
                     /locus_tag="NBI42_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1777304.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLEKQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKDMKDFKLNDIQNHTLLVTEKGCSYREQLEKILYKYSFSTKQMIEFL
                     GIESLKKHLKNSDGIALLPEFIVADELQNKSLFQIDIDVDIPNLETTLIINPESSKQV
                     LESFVKDVFL"
     gene            6221..6823
                     /locus_tag="NBI42_000868"
     CDS             6221..6823
                     /locus_tag="NBI42_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777305.1"
                     /translation="MLISLVIPVFALLVIGGIIWAIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVIFTFGIAGIIFLMPYGLCF
                     LPLYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            complement(7115..8029)
                     /locus_tag="NBI42_000869"
     CDS             complement(7115..8029)
                     /locus_tag="NBI42_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1777306.1"
                     /translation="MKQSVNFKSKTNLLAANLYTPEQFDESKKYPAITVCHPGGGVKE
                     QTAGMYAEKLAEFGYVTVVYDASHQGESEGSPRYLEDPFARTEDVRASVDYLTTLDFV
                     DNDRIGALGVCAGGGYTVSAARTERRIKALATVSMVDIGALFRKGPGDVVSIDDQLAF
                     MTQVADQRTAEANGADYGITGYVPEEIDDSMPENSTMVQGHDYYLTARGQHKNAPNKL
                     LLRSFSEVLTFAPFTYIDELLTQPFLAIAGTEADTIEYSVDAVEKAAGDNNELYKIEG
                     ASHVDLYDIPEYVNQVLPKLESFYKETL"
     gene            8208..8762
                     /locus_tag="NBI42_000870"
     CDS             8208..8762
                     /locus_tag="NBI42_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCD1777307.1"
                     /translation="MNVGDLRVVKTRASIKKAFMTLLFEKDFDTISIKEITEFAQIGR
                     KTFYLHYIDKYDLLDQIVSEKLIELEQISEAKKSLGIQEGTQLWFEFFENNRTFFMKI
                     FNINNASNYKKKLLHFIEEEFKKKVPTRVATEKGLDYDLYINFISNGIIGLLDIYLNS
                     SENTEEQEKITLQVSRLLSLYDLI"
     gene            8993..9724
                     /locus_tag="NBI42_000871"
     CDS             8993..9724
                     /locus_tag="NBI42_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCD1777308.1"
                     /translation="MQNRNDFIEKLLDRAQIKEGMRVLDIGCATGEVTQLIAKRVGAN
                     GEVVGVDVNESLLKIANENNQYNNVSYQYSDIYQLPETMGYFDAIVGRRVLMYLPDAE
                     KCLQILKSILKPEGILCFQESDAINTGVGADALPLHQSATQWIWETVKQEGGNIHIGQ
                     NLYNLFNKNGMHVVDYFAEAVIQTSMDNDLAWLVDVMLPRMKAHGVINDDFSLDEFKL
                     NLEHEAMNNQCAFIRDMAFGIIGKA"
     gene            9879..10361
                     /locus_tag="NBI42_000872"
     CDS             9879..10361
                     /locus_tag="NBI42_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777309.1"
                     /translation="MKKFNNWILNAISGSQTDKNGTTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTEIIIVLILIESLIGLRFLKEYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGITDLGNTRSDLIVWSIGGIIFGLVCYTMEDKK
                     "
     gene            10566..11552
                     /locus_tag="NBI42_000873"
     CDS             10566..11552
                     /locus_tag="NBI42_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HCD1777310.1"
                     /translation="MKKCIKTLFLSVILVVMSGLYHSAHASDSLSKSPENWMSKLDES
                     KHLTEINMPGSHDSGSFTLTDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGNLNLQVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYPWPGYDDIVSEIIDSNK"
     gene            11773..12543
                     /locus_tag="NBI42_000874"
     CDS             11773..12543
                     /locus_tag="NBI42_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1777311.1"
                     /translation="MMKRLNKLVLGIIFLLLVISITAGCGIGKEAEVKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSQMAIQNKGEALKIKGMLLKINRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMIDNKFIPAEEVKDEKIKTEIQNFKFFAQYS
                     NFKDLMNYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKFILKGTG
                     DLKGSSVGYKKIEFTFVENENENIYFTDSLYFEPSEDK"
     gene            12546..13922
                     /locus_tag="NBI42_000875"
     CDS             12546..13922
                     /locus_tag="NBI42_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777312.1"
                     /translation="MTKKEYKIDPGKIKSNSEETSAIASISYEIENANDNGLNKARIT
                     EQVTYLKNQDKFPKNLDYIESYTDPSTGTTATAFLNKDTGKVTVGMAGTNFHGDQIKK
                     VALSSMSPLLFPPSKQDMKDVRGTMKDGAADLAIGVGLVNYKGKHFANTQQFIENLQK
                     KYEIDTVTGHSLGGRDAIFLGLRYNIKNVVAYNPAPLEVKSIRNKLGGQLFRNTTFPD
                     EKYLKELMDNYDGDITKVITQKDGLDYLVKHTDHLTCGDVLRINNGQGHAMENFLGEK
                     EQREIIEELMLVKGYRDANDKAFKALKKNTEKKLGKIDEIKSKLLQTNGGVLSSSQQK
                     LLETLVAFSVAEGLSKMVDQELQQLKNMFNLMDEKFEANWKDAQEASDIVGKHLSYPE
                     KVSALDNGGVNESKLATEPHNEIKEKLNKITDLSKKYNSYLKQIEKSINEIVAKDQQL
                     AGQIGDLI"
     gene            13922..14248
                     /locus_tag="NBI42_000876"
     CDS             13922..14248
                     /locus_tag="NBI42_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777313.1"
                     /translation="MFEEYKKIDDLENAYEIELKRIEREIQNLSDLKYHLRRENEQSY
                     DAFLYLKNKMNYSEESNAKVRRLVEEFDYEADSYIRQKELKLEDYKEEIRREYIQQSE
                     KIMEAK"
     gene            14252..14563
                     /locus_tag="NBI42_000877"
     CDS             14252..14563
                     /locus_tag="NBI42_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777314.1"
                     /translation="MGIPDPLSNPMSNPLLGDSWMVGDLKKRQEKELITTVIAPEHDE
                     KHRENKSKMKKSEKQLINQLINHGHTFKSDFKNVAKGDWVNKAMQDLDKLGNELKKIM
                     N"
     gene            14815..17052
                     /locus_tag="NBI42_000878"
                     /pseudo
     CDS             14815..17052
                     /locus_tag="NBI42_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            17203..18381
                     /locus_tag="NBI42_000879"
     CDS             17203..18381
                     /locus_tag="NBI42_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1777315.1"
                     /translation="MNQQLIEALKSKEDKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKAMIENGVLDGVDHV
                     LGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEALYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEADLEFGVEICEPQPPSED
                     FAYYAKERPSAFIYTGAAVEDGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            18383..19771
                     /locus_tag="NBI42_000880"
     CDS             18383..19771
                     /locus_tag="NBI42_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1777316.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGADM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRIIQGVSAACIMPSTLAIMKTYYEGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEVTNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAVVVVAFFIFLKVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVIIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIIAFIAILFA
                     IPNDDKRAKDAK"
     gene            20118..21581
                     /locus_tag="NBI42_000881"
     CDS             20118..21581
                     /locus_tag="NBI42_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCD1777317.1"
                     /translation="MKKILLTGASGYIGSHLMEKLKENHDIIAISRNIDNKKNERNVT
                     WKSADLFDLAEINDVMEGIDTAIYLVHSMMPSAKLTQASFEDMDAILADNFAKAARQN
                     GVKHIVFMSGLIPNDDQLSPHLRSRLECERILGSYGVPVTTLRAGLIIGSKGSSYPIL
                     KTLVERLPALVLPKWAYNQTVPVAIDDVIQGLYKVVSRSPKVNEAIDIGGPLHMTYKD
                     LFKQTAEVLDKRLPMIDLPIIPIWLSKFWVKLISGVPKEMVYPLMDSLIHNMTRDQKR
                     IVEGISIGQMDYKESVQQALEEASTSKKKQTSTIKTDVKDVRAISRVVLPKHMNMSAL
                     AEIYANFLNKITLNIVNSNFDEEAFIISLPLLKKELLLLEKDTQTSDEDRVSYKIIGG
                     DFALAHNGGNARLEFRRLPNSDSCIIALQEYEPTLPWWVYKYTQAKVHKSVMNLFKFN
                     LYMKRHNQKLSSVTKQGLIVGAVLGALIVKSYKSRNY"
     gene            21644..22138
                     /locus_tag="NBI42_000882"
     CDS             21644..22138
                     /locus_tag="NBI42_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCD1777318.1"
                     /translation="MTFKRLLKTIVKVVTPLLGGRLIGKYAVQNARKDYKDNAKPLLS
                     PPGYIFPIVWSILYTTIGIAYAIISGKSNNAKVKTIYYAQLSLNYLWSILYFKFKLRF
                     AALIESFILLTAVIMMTVKFFKVKRIAGIILIPYMLWSTFASYLTVGNWLLNKDNPRY
                     TEQK"
     gene            complement(22364..24025)
                     /locus_tag="NBI42_000883"
     CDS             complement(22364..24025)
                     /locus_tag="NBI42_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCD1777319.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSSNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(24352..26127)
                     /locus_tag="NBI42_000884"
     CDS             complement(24352..26127)
                     /locus_tag="NBI42_000884"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCD1777320.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENIPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKKAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKVDVTTSQKIAREILIHRHGKAE
                     SIKLTVDDLVFVTNGSITESSRYGDNNTPAPPTNELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPEKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGVPEVINSPFDLRVLMDAVYELNDHQDLREITKDSKMQK
                     LALSGFLKKVKGTYIETLLKEHKLL"
     gene            26479..26952
                     /locus_tag="NBI42_000885"
     CDS             26479..26952
                     /locus_tag="NBI42_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCD1777321.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVMNYSKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINGAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIQTIKLFLSTLNLE
                     IMSYISIFMILEIWQIQHNHQLNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            27214..>27902
                     /locus_tag="NBI42_000886"
     CDS             27214..>27902
                     /locus_tag="NBI42_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCD1777322.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISEDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILT"
BASE COUNT         9803 a         4117 c         4872 g         9110 t
ORIGIN      
        1 acttatacta atcactttgt tatttggtta cagttgctat ttgttgttgg aagctaaagc
       61 agagacacaa aatgatccaa atataagtga attaaacaaa gctagtcaat atacgggttc
      121 atggcataat atatggtatt tatataatag cgatccagtc aaagctaaaa aaactaagct
      181 gagtgacaaa tttttgagtc atgatttcat tgttccaata aatagccctg gtcattacga
      241 ctatgttaaa actgagttaa aagacagtac gatggcgagt tcatttgatg ggaaagaagt
      301 cgatattttt ggcgtcaatt attttcatca atgctatttt tcaaatgaaa atatacagtg
      361 tgatagtaat caaggagctg gaagtaaaaa aacttgtatg tatggtggta taacattaaa
      421 tgagaacaat accaataata gaattcagcc aattgttgta aaagtttacg agaatgacag
      481 cgttacgctt tcttttgata tcaatattga taaagagact gtaaccattc aagagttaga
      541 ttataaagtg agaaataaat taatttctaa aatcaattta taccatttag gtggcacttc
      601 atatgaaacg ggatacatta aatttataga aaatgctaat cgttattatt ggtatgatat
      661 gatgccagac cctagcttta ctcagtctaa atatttgatg atatatcgag gtaatgaaac
      721 agttgaatca gccaaaacgg aaatagaagt gcacttaact aaaaagtaga catagagcaa
      781 tttattaata ataattgatg gctaaattaa agtaaataaa aatattttaa tgaattaaag
      841 cgactaggaa aagttgcttt tttaattgct tctttaaaaa gtaggtgcat aagactttaa
      901 cacaaacaaa aaaggaggaa acttaaattc ctcctcaacc tttattactc atactataat
      961 tctagtttaa cgtcttcgtc catttgggct tcaaattcat ctagtagtgc tcgtgcttct
     1021 gcaattgatt gtgtgttcat caattgatgg cgaagttcac tagcgcctct tatgccacgc
     1081 acatagattt taaagaatct acgcaagctc ttgaattgtc gtatttcatc tttttcatat
     1141 ttgttaaaca atgataaatg caatctcaac agatctaata gttccttgct tgtgtgttcg
     1201 cgtggttctt tttcaaaagc gaatggattg tggaaaatgc ctctaccaat catgacgcca
     1261 tcaatgccat atttttctgc aagttcaagt cctgtttttc tatcgggaat atcaccgtta
     1321 attgttaaca atgtgtttgg tgcaatttcg tcacgtaaat ttttaatagc ttcgattaat
     1381 tcccaatgtg catctacttt actcatttct ttacgtgtac gaagatgaat ggataaattt
     1441 gcaatgtctt gttcaaaaac gtgcttcaac caatctttcc attcatcgat ttcatagtag
     1501 ccaaggcgtg ttttaacact taccggaagc ccacctgctt tagttgcttg aataatttcg
     1561 gcagcgacgt caggtcttaa gattaagccg gaacccttac cctttttagc aacatttgct
     1621 acaggacatc ccatatttaa gtcaatgcct ttaaagccca ttttagctag ttgaatactc
     1681 gtttcacgga actgttctgg cttatctccc catatatgag cgaccatcgg ctgttcatct
     1741 tcgctaaaag ttaagcgtcc gcgcacacta tgtatgcctt cagggtggca aaaactctcg
     1801 gtatttgtaa attcagtgaa aaacacatcc ggtctagctg cttcacttac aacgtgtcga
     1861 aagacgatat ctgtaacgtc ttccattggc gccaaaataa aaaatggacg tggtaattca
     1921 ctccaaaaat tttctttcat aatatattta taccctcttt ataattagta tctcgatttt
     1981 ttatgcatga tgatattacc acaaaagcct aacttataca aaaggaattt caatagatgc
     2041 aaccatttga aagggaagtc taagagtagt ctaaaataaa tgttgtggta agttgatcaa
     2101 tatacaaatc aaggattatc gtattagatt gttcattatt aatgatacac tacttattaa
     2161 tatgattcag aattttcttt agctacatat acagtaatgt gatttatacg ttatcttata
     2221 acaaagacaa atttataaag gtgatattat ggaagattta aagcaatctt taaaaggttt
     2281 aggttggtat gacttatttt ttatagtacc tatgtttcta ctattcgtgt atctgccgaa
     2341 ttataatttt ataactatat ttattaacat tgttatcatt attttctttt ccataggttt
     2401 gattttaact acgcatataa ttatagataa aattaagagc aacacgaaat gattcattaa
     2461 tacgaatgtg attaaacata aaactgaagg agcgattaca atggctactg aaaaagatat
     2521 aaatgatttg tttttaaatc atgtgaattc aaacgcggtt aagacaagaa agatgatggg
     2581 agaatatatt atttattatg atggtgtggt tataggtggt ttgtatgata atagattatt
     2641 ggttaaagcg actaaaagtg cacgtcatca atttcaagat aatacattag tatcgtcgta
     2701 tccaggtgct aaagaaatga tattaattcc agacattacc gaagcaacaa atctcactaa
     2761 tttatttgag tgcataaaaa atgatttgaa aaagtgaagt tcgaatggag agaattaagc
     2821 acatcgacaa atgatcgcat gttaaaagaa gagtgacggt cactcttctt tatgtgcata
     2881 ttttattttg tctgttttgt taacaagcag cagtgtaaca aatatgagta aggataaaat
     2941 gagtataata tagaaaccga atttatcatt aatttcatta atccatcttc ctaaaaatgg
     3001 agcaatgaaa ctttgcagca acaatgaaat tgacgtccat atcgtaaatg agcgaccgac
     3061 atatttatct gaaacagtgt tcattatagc tgtattcata taaattctga ttgatgaaat
     3121 tgagtagcct agtataaatg atcctatgaa taagtaaaat gctgagttta tccaaataaa
     3181 tagtgctgaa tttatgacta atatgaaata taacaaaaat atcaatactt tagttgagat
     3241 tttcttcgaa agaatagctg aaattaaacc tgcacataat cctccaatgc catataacat
     3301 atctgaaaaa ccaaattgta cagacgaaag ttttaaaaca ttatatacat atcctggtaa
     3361 tgatatgtta aagatcattg taaacaccat tggtatgatt gaaataactc caaaaataaa
     3421 tatcatcatg ttgtctttta aaaatttcca tcctaataaa tattcttgca ataagctatt
     3481 tgttgattct tcctctgaat gagttggttt atctacatgc aatctaaata acataaaaat
     3541 gctgattaga aacatcatta tagtcatcgc tataattaga gtgaatccat ttatttcata
     3601 taatattcct gataatccac ctgcaataaa catacctgtt tgcaaactaa tctctaatag
     3661 agaatttgca tctgtatatt gatctggttt taatatctgt ttaactaaac ttctagatgt
     3721 tgccatatac gtagtccaac ctatcccatt aacaatcgca aatccaatca ctaaataggt
     3781 ctcaaagcct atcattacga gtgctatgac aatgagtaaa tataatatta cctgaagaag
     3841 ataggtgatt aaaataatgt ttcgtctatt atatttatcg gctagtccac ctataatagg
     3901 agaagctaag aaaccggata atacatttaa agctaacata atacctaata gttgggagtc
     3961 gttagttttg tcgattaaat accaattagc ccctacagta ctaattccaa ctccgaatgc
     4021 tgaaataatt tcagctaaaa agaacaatct gaagttttta attttcaaca aatcagtcat
     4081 aaatatcacc ttttttcaat gcaagattta tatttgcgag ctcttttata aattgagagt
     4141 cgtttaaaat atcgtatggt ttattgcctt cgccaatcaa aaatctatag ttagtaatat
     4201 tcataaactc aaatattaat ttgaattggt tgactaaagg ttgtgctttg gtatgcggat
     4261 tgtcgccacc tatgattaaa atcaaatact ttttttgtga catgatttct ttgaagttgt
     4321 ctatttgcgt atctcgcaat gactcggtcc atctatcaat gaagagtttt aaagacgcac
     4381 tcatggagta ccaatagagt ggtgttgaaa aaataataat atctgattct aaaactttga
     4441 ttaaaatttg ttcgtaatca tcattatgaa aattggcacc tttgctatga cgattatctg
     4501 taaccttttc aatgttactt tgatataaat tcacaaagtt gacatttaaa ttcttcaata
     4561 gattctctac tgcgatagct gaattgccat cttttctact acttccaaat aaacaagtaa
     4621 tcatagtcat aactcctttc gatttacctt aataataata tttaatatat tattattaaa
     4681 tcagaattct tagaatgtag aattcgataa aagtgaatcc taaaggaggt actactttga
     4741 acttaaacat tttaaaatca tttttagtaa ctagtgaaac aaagaacctt actaaagcat
     4801 cagaattact taactattca cagtcaactg tatctacaca tattgaaaaa ttagaaaagc
     4861 aattagatgt taaattattt tatagaaaaa aatatggtat ggaactaacg gaagaaggct
     4921 tagcatacgt taaatacgct aaagtgattt tagatagtaa tagcgaatac gagagagaaa
     4981 taaaaggact ttacaataag aaggtaaata taagtattaa catgcaagaa agtcagtatt
     5041 tgtatcgcta ctataataag attagtgaat ggttagctga acacccatat gtaaacttga
     5101 agtttaaatc cgcacattct aatttctata ttaaagaaga aattgctaat ttcaaatcgg
     5161 atattagcct tatcacagac gaaaagatta ttaatagtaa cttaactgct attcctataa
     5221 ctgaagaacg tttaatattt gttactaaca aagatatgaa agatttcaaa ttaaacgata
     5281 ttcaaaacca tacattactt gttactgaaa aagggtgtag ttacagagaa cagttagaaa
     5341 agattctcta taaatatagc ttttcaacaa aacaaatgat tgaatttcta ggaattgagt
     5401 cattgaaaaa acacttgaaa aattcagatg ggattgcgct attgccggag tttattgttg
     5461 cagatgaatt acaaaataaa agtttgtttc aaatagatat cgatgtcgat attccgaatt
     5521 tagaaacaac tttgataatt aatcctgaat ccagtaagca agtactagaa tcttttgtaa
     5581 aagatgtctt tttataaata ttggtgaaaa cttgtagtta tggtgaaact caaagataat
     5641 catttattgg taagggcata cgaacaatta aaatttccaa acaaggtaca cgagtgcttc
     5701 tgaatttatc ggcacgcttt tacatagatc aaaagaacgc gcactaaaat aatcattata
     5761 tctatgtaac ttacatcttt gatactacag aagttcgtgc atttgttcaa tcgtctcata
     5821 accattaaat gccactaagc acgacctgac tgtttcacac tttatgaaaa tctttaaata
     5881 gcgttaatta tgaatgaaga catctataac atttatttat cttataaaat agtacaatta
     5941 caaagcacta caacggcgac tggactcacc ttaaatcata acgacaaccg ctaaaaattg
     6001 atcgcaaaac taatcattaa ttgccatact taactatttt gaaaagtatc aatttcacat
     6061 tataaaatta ttgagtatct agatttattg ttatacacaa acagtatttt tgttatgata
     6121 gagttaataa atataataaa ttgaataagt aactttacaa aactagaatg attcaattca
     6181 atcataattg tttgaaaata gaggtgagca ggtgaacgat atgttaatta gtctcgtaat
     6241 cccagttttt gctttgttag ttattggtgg tattatttgg gcaattatag aaggtatagt
     6301 acatatttca aaaaagaata aagcaattga taattttttt aaccaagtta ataaagtaag
     6361 tgagacatat aaattcgcta ctactttttt atttctaatc ttggctacgg ctggtatttc
     6421 tcaattttat ctatattata tagtatcagc gtttcttttt tggcttgtca tatttacctt
     6481 tggtattgca ggtatcattt ttttaatgcc atatggctta tgcttcctac cactttataa
     6541 gcaaaaaaag aaaaaacaga catttaaaaa atacatggtt tacactacga ttggtttgtc
     6601 aatttgtcta ggcttatctc tagttttagt tcacactacg aaaatttata tggacgaagg
     6661 tggcgtaaga tactattacg gtagttttgt aatgaaacaa gcgggcggtt atgcttattt
     6721 agctttagcg gtactttcaa cgctgttaat tgttgcgaaa aaagctacaa ataaaaataa
     6781 agaaatcgaa accgtcgaca atacaaatat aacggaaaga taattaaggg agtgctcatt
     6841 caggagtgct cttttttgat gttcaaattt agttggtaat gaagaagcag aaacatttgt
     6901 gagtggaaaa taaaagttaa gctgaaatcc tatcgaacgt atctgtaaag taaaaaatta
     6961 acgccacaat tttcatcgta attaaaaaca gacattctag taacatgctt tttcaaaaac
     7021 taatctaata atacgtccat ctataaaata tttctacgta ctaaaaaagg cggagtaaat
     7081 cgtccgcatc aatgcacaca accgcatgtt gtttttatag agtttcttta tagaatgatt
     7141 caagttttgg taacacttga tttacgtatt ctggaatatc atataaatct acgtgtgatg
     7201 caccttcaat tttatagagt tcgttgttgt cgcctgctgc tttttcaaca gcgtcaacgc
     7261 tatattcaat cgtgtctgct tctgtacctg cgattgctaa gaatggttgt gtcaacaatt
     7321 cgtcaatata tgtgaaaggt gcaaatgtta atacttctga aaaacttctt aataatagtt
     7381 tgtttggtgc gtttttatgt tgaccacgtg cagttaaata ataatcatgc ccttgtacca
     7441 ttgttgaatt ttcaggcata gaatcatcaa tttcttcagg cacatagcca gtgataccat
     7501 aatctgcacc atttgcttca gctgtacgtt gatctgcaac ttgtgtcata aatgccagtt
     7561 gatcatctat tgatactaca tcgccaggac ctttacggaa taaagcgcca atatctacca
     7621 tacttacagt tgcaagtgct ttaatgcgac gttctgttct tgctgcactt actgtatatc
     7681 caccacctgc acaaacgcca agcgcaccaa ttctgtcatt atccacaaag tctaatgttg
     7741 taagataatc aactgaagct ctaacgtctt ctgttctcgc aaatggatct tctaaatatc
     7801 taggagagcc ttcactttca ccttgatgtg atgcatcata tacaaccgta acgtagccga
     7861 attccgcaag tttctctgca tacatacctg cagtttgttc tttaacaccg ccacctgggt
     7921 gacaaactgt aattgctgga tattttttgc tttcatcaaa ttgttcaggt gtatataaat
     7981 tcgctgctaa taaatttgtt ttacttttaa aattaactga ttgtttcata gattaaatgc
     8041 ctccatcttt ttatataagt cattcatttg ttgtagatat ttattactct tgtgtgtata
     8101 atgccatact agagtattta aatgtacaat tcaattaaca aggaagcaca tatgtgtttt
     8161 aatacacagt tttataaaaa tgggttttaa aagaaaggag tattattatg aatgttgggg
     8221 atttacgggt tgttaaaacg agggcaagta ttaaaaaggc atttatgacc ttactttttg
     8281 aaaaagattt tgatactatt tcgattaaag aaattactga atttgcgcaa attgggagaa
     8341 aaacatttta tctacattat attgataaat atgatttact agatcaaata gtatcggaaa
     8401 aattgataga acttgaacaa atttctgaag ccaaaaagag tctgggtatc caagaaggta
     8461 cacagttatg gtttgagttt ttcgaaaata atagaacatt ttttatgaaa atttttaata
     8521 tcaacaacgc ttcaaattat aagaaaaagc tgcttcattt tattgaagaa gaatttaaga
     8581 aaaaagttcc gacacgtgtt gctactgaaa aaggcttaga ctacgactta tatattaatt
     8641 ttattagtaa cggtattatc ggactactag atatctatct aaacagcagt gaaaatacag
     8701 aagaacaaga aaaaataaca ttgcaggtgt cgcgcctact atctttatac gatttaattt
     8761 aaaactaagc tatctatttt gaatataatt gcgtgtagca gatatagtat gtcacttctt
     8821 gagagaattt tattataata gttttatgag ttgatattgc agtgacaaga gctaaaaaag
     8881 aattttatgt aattggcgac atgcaaagaa tacagtcgaa accattttat gagacgattt
     8941 ttaaaaaaat gtagaataac atacaaaaaa gtatatagag ggagttattt tcatgcaaaa
     9001 tagaaatgat tttatagaaa agttattaga cagagctcaa attaaagaag gcatgcgtgt
     9061 actggatata ggttgtgcga ctggtgaggt cactcaatta attgcaaaac gtgtgggggc
     9121 gaatggtgaa gtcgtcggtg ttgatgtgaa tgaatcatta cttaaaattg caaatgaaaa
     9181 caatcaatat aacaatgtat catatcaata ttctgatata tatcaattgc cagaaactat
     9241 ggggtatttt gatgcgattg ttggtagaag agtattaatg tacttaccag atgctgaaaa
     9301 gtgtttacaa attttgaaat caattttaaa acctgagggt atattatgtt ttcaagaaag
     9361 tgatgctatt aatacaggcg ttggtgcaga tgcactacct ttacatcaat cggcaactca
     9421 atggatttgg gaaacagtga agcaagaagg tggcaatatt catataggtc aaaatttata
     9481 taatttgttt aataagaatg gaatgcatgt tgtagattat tttgcagagg cagtgattca
     9541 aacgtcaatg gacaatgatt tagcatggct tgtagatgta atgctaccaa gaatgaaagc
     9601 gcatggtgtt attaatgatg atttttcact agatgagttt aaattaaacc ttgaacacga
     9661 agcaatgaac aatcagtgtg catttatacg tgacatggcg tttggtatta tcggaaaagc
     9721 ctagtttaaa aacgctgaaa gcagcatatt atcatcgcca ttgttttagt ttgtgaagtg
     9781 attaatggaa tttgaaaata acagcatttc tataggctaa tcaagtgata cttaattagc
     9841 gagcaattta tacattttaa aaggagtaaa attgaataat gaagaaattt aacaactgga
     9901 tattaaatgc aataagtgga tctcaaacag acaagaatgg aacaactgaa gaattaaaag
     9961 gggcaaaatt tatcatttta tatgcatatt caatgctcgt tttgcttgcg ttagtaattt
    10021 ctaacatatt cattcacatt ttggagccta aactttcaat taccactgaa atcatcatcg
    10081 ttttgatttt aattgaatca ctaattggac tgcgtttctt gaaagagtac gatgttaagc
    10141 gtggcaaaga taaagaaaat aagaaaaata gtaaggattt cgttaaacta aaatcaattt
    10201 tagtagcaat tttatttaca tcattggcgc tgacagcagg cactgtagct gatatatacg
    10261 gtatcactga cttaggaaat actagaagtg atttaatcgt ttggagcata ggtggtatta
    10321 tatttggcct cgtatgttac acaatggaag ataaaaaata atgataagga gctggcgatt
    10381 ataaagctag ctccttttta aacttatata tgtaaagaac tatcctaagg gtttttaatc
    10441 atatgtcaat aattcctata atacattatt aaaacatcaa ttaaataagt tttaaaattt
    10501 tacacatatt tttatttaaa aaagatgtat aattaatata ttaaatatag aaagaagttg
    10561 atattatgaa aaagtgtatt aagactttgt ttttaagtgt cattttagta gtgatgagtg
    10621 gtttatatca ttcagcacat gcgtcagatt cgttgagtaa aagtccagaa aattggatga
    10681 gtaaacttga tgaaagcaaa catttaactg agattaatat gccaggctca catgatagtg
    10741 gctcattcac tttaactgat ccggtaaaat cagtttgggc aaagactcaa gataaagatt
    10801 accttactca aatgaagtca ggagtcaggt tttttgatat tagaggtaga gcaagtgctg
    10861 ataatatgat ttcagttcat cacggcatgg tttatctgca tcatgaacta gggaaatttt
    10921 tggatgatgc taagtattac ttaagtgctt atccaaacga aacaattgtg atgtctatga
    10981 aaaaggacta cgatagcgat tctaaagtta cgaagacatt tgaagagatt tttagagaat
    11041 attattataa taacccgcaa tatcaaaatc ttttttacac aggaagtaat gcgaatccta
    11101 ctttaaaaga aacgaaaggt aaaattgtcc tatttaatag aatggggggt acatacataa
    11161 agagtggtta tggtgctgac acgtcaggta ttcaatgggc agacaatgcg acatttgaaa
    11221 cgaaaattaa taatggtaac ttaaatttac aagtacaaga tgagtataaa gattactatg
    11281 ataaaaaagt tgaagctgtt aaaaatttat tggctaaagc aaaaactgat agtaacaaag
    11341 acaatgtata tgtgaatttc ttgagtgtag cgtctggagg cagcgcattt aatagtactt
    11401 ataactatgc atcacatata aatcctgaaa ttgcaaaaac gataaaagca aatgggaaag
    11461 ctagaacggg ttggctgatt gttgactatg cagggtatcc gtggcctgga tatgatgata
    11521 tcgtaagcga aattatagat agtaataaat aaggattcaa taatgatatt aagacgagta
    11581 tgaaaatagt tagattctaa ttattttcac tactcgtttt tattttgaaa ataagtaata
    11641 attcaacaat attataaatt gaacagattg tttgtggaat ttttgataat attaaagtga
    11701 aaaagtgtta taaattgata ttaatatgta atttgcaaaa gtaaatcatt ttaaaaagaa
    11761 gagagttgta agatgatgaa acgattaaac aaattagtgt taggcattat ttttctgctt
    11821 ttagtcatta gtatcactgc tggttgtggc ataggtaaag aagcggaagt taagaaaagc
    11881 tttgaaaaaa cattgagtat gtatccaatt aaaaatctag aggatttata cgataaagaa
    11941 ggctatcgtg atgatcaatt tgataaaaat gataagggaa catggattat aaattctcaa
    12001 atggcaattc aaaataaagg agaagctcta aaaataaaag gcatgctttt gaagataaat
    12061 agaaatacaa gaagtgcaaa aggattttac tatactaatg agataaagac ggagaaatac
    12121 gaagtagctc aggataatca aaaaaaatat ccagttaaaa tgattgataa taaattcatt
    12181 cctgctgagg aagttaaaga tgaaaaaata aaaacagaaa tccaaaactt taaatttttt
    12241 gcacaatata gtaattttaa agacttaatg aattataaag atggggatat atcatacaac
    12301 ccagaggtgc cgagttattc agcaaaatat caattaacca atgatgatta taatgtaaaa
    12361 caattacgta aaagatatga tataccaaca aataaagctc caaagttcat attaaaaggt
    12421 acaggagatt taaaaggttc atcagttgga tataaaaaaa ttgaatttac ttttgtagag
    12481 aatgaaaatg aaaatattta ctttactgat agtctatatt ttgagccgag tgaggataaa
    12541 tagttatgac taaaaaagaa tacaaaatag atccaggaaa aataaaaagt aattcggaag
    12601 aaactagtgc aattgctagt attagttatg aaattgaaaa tgcaaatgat aatggtttaa
    12661 ataaagcaag aattacagaa caggttactt acttaaaaaa tcaggataag ttccctaaaa
    12721 acttagacta catcgaaagt tacacagatc ccagtacggg gaccacggca acggcatttt
    12781 taaataaaga tacaggtaaa gttactgtag gaatggctgg gacaaacttt cacggcgatc
    12841 aaattaaaaa agtagcactt agttcgatgt ctccgttatt attcccaccc tctaagcaag
    12901 atatgaaaga tgtacgaggc actatgaagg atggtgctgc ggatcttgct attggagttg
    12961 gattggtcaa ttataaagga aaacattttg caaatacaca gcagtttatt gagaatttac
    13021 aaaaaaagta cgaaattgat accgttactg gtcattcttt aggagggaga gatgctatct
    13081 ttcttgggct tcgttataac atcaaaaatg ttgtggcata caatcctgct ccgttagaag
    13141 taaaaagcat tcgtaataaa cttggtggtc aattgtttag aaatacgaca tttccagatg
    13201 aaaaatattt aaaagagtta atggataatt atgatggaga tattactaag gttataactc
    13261 aaaaggatgg attagactat ttagtgaagc atacagatca tttaacttgt ggtgatgtgt
    13321 tacgtataaa caatggtcag ggccatgcga tggagaattt tttaggagaa aaagaacaac
    13381 gtgaaataat cgaagaacta atgttagtaa aaggatatcg cgatgcaaat gacaaagcat
    13441 ttaaggcttt gaaaaaaaat actgagaaaa aactcggtaa aatagatgaa atcaaatcga
    13501 agttactgca aacgaacggt ggtgtcctct cgtcttctca acaaaaattg cttgaaactt
    13561 tggtggcatt ttcggtagct gaaggactaa gtaaaatggt tgaccaagaa cttcagcagt
    13621 tgaaaaatat gttcaatctt atggatgaga aatttgaagc aaattggaaa gacgcacaag
    13681 aagctagtga tatagtaggt aaacatcttt catacccaga aaaggtaagt gcattagata
    13741 acggtggtgt caatgaaagt aaacttgcga ccgaaccaca taatgaaata aaagaaaaac
    13801 taaataaaat tacggactta tctaagaaat ataattcgta tttaaagcaa atcgaaaaaa
    13861 gtattaatga aatagttgct aaggatcaac aattggcagg gcaaataggt gatttaatat
    13921 gatgtttgaa gaatacaaaa aaatagatga tcttgaaaat gcatatgaaa tagagttgaa
    13981 acgtattgaa agggagatac aaaatcttag tgatttaaaa tatcacctta gaagagaaaa
    14041 tgaacaaagt tatgatgcat ttttgtattt aaagaataaa atgaactata gtgaagagtc
    14101 aaatgctaag gtgagacgac tagttgaaga atttgattat gaagcagata gttatattag
    14161 acagaaagag ttaaagttag aagattataa agaagaaata agaagagaat atatacagca
    14221 atctgaaaaa ataatggagg cgaagtagct tatgggaatc cctgatccgt tatctaatcc
    14281 tatgtctaat cctttactag gagatagttg gatggtaggt gatttgaaaa aaagacagga
    14341 aaaagaacta atcacaacag tcatcgcacc tgaacatgat gaaaagcatc gtgaaaataa
    14401 aagtaaaatg aaaaaatctg aaaagcaatt gatcaatcag ttaattaatc atggtcatac
    14461 atttaagagt gattttaaaa atgtagctaa aggtgattgg gttaataaag ccatgcaaga
    14521 cctagataaa ttaggcaatg agcttaaaaa aataatgaat tagttgaatt gtcacatact
    14581 ttttcaaact aacaacgaac taaaaaatct atataaatat gattaaaaga agtcataacc
    14641 attcgtaaag gatggttatt tttaatagta aacaaaattg ctgctaactt acattttaaa
    14701 ccgagtgagg ataaataacc tcctgcttcc cacacgattc aacacgaatt tcaaatatgt
    14761 cgctgtaaaa taatgtaaaa taaacgtatt catattactg aattgaggga tagtatgcaa
    14821 cgtgattatt taattcgagt agaaactgag agtatgtcag atttcaaaag gctcaatggt
    14881 ttaatgattg gttttgttat taaaggtgag gcacatattt atgatgaaaa taatatgacg
    14941 caatgcaaca gtggcgacat tttcatcatt aaccaccgcg acttgtatcg atttcaactt
    15001 caacaagatg gcatcatatg ttatatccaa ttccaaatga aatatttagc agacaagttt
    15061 gatgatgtgc attgtctata ttttcactta acagatgcga ccacaacaaa aaatatacat
    15121 caactgagaa atataatggc aagactggtt tcaacacata ttcgacataa tgagttgtct
    15181 aaattgactg agcaacaact tgtgattcag ttgcttatgc atatgattca ttatgtcccg
    15241 cgtacatatc attcgaacca aagtatctta aatgatgata aagtgaatca agtatgcgac
    15301 tatatcgagt tacattttca tgaagactta agcctttcgg aattaagcga atatgtaggg
    15361 tggtcagaga gccatctgtc taaaaagttt acagaatcgc taggtgtagg attccagcat
    15421 ttcttaaata cgacgcgaat tgagcatgcg aaactcgatt tgacatacac agatgaaacg
    15481 attactgata ttgcattgca aaatggcttt tcaagtgcag cgagctttgc gagaacattt
    15541 aaacacttta cgcatcaaac gcctaaacaa tatcgaggtg atcgtccagc aatcactgaa
    15601 aatcaacaat cggcacaaca taattagcac gaccgtgaat tgatattact tttaaatgac
    15661 tacattgaag aaatgaatca tttcattgaa gatattgaaa aggtgaacta taaagagatt
    15721 acctttcaac caaccaatca ccaactcaat caatttaatc atattattca agtgggctat
    15781 ttgaggaatt tgctcaatac acagtatcaa tcacagttgc ttacatgtca tcatgatttt
    15841 catgtcaatg aagtattagc atatgatgtg atgccatata ttatgaaaaa gctcaatgcg
    15901 ccattcacgt atgatgcaga aatttcgaat atattttatg atatcgattt gtgtttagac
    15961 tttttattag ataataactt tagtttgacc atgcatttgg atcattatga ctcacgggat
    16021 tatatcgatg catttaaaat ttttatccat cacgttgccc tgcatgtcag tcatagaaaa
    16081 gatttgaagt tcaacttgta tgtgacgaca ttgcacacgt ctttgattga aatgattgat
    16141 tattttaaag cattattccc taatggtggc ttgtacattc acttagatca agctacggaa
    16201 agacatctac cattgttgaa acgacttgag ccacacatcg accattttgt atttgatgcc
    16261 aattcaaatg atgctgttga ttttaataaa atgaatgatg atgaatttaa aaccgcaagt
    16321 caaatgatta ttaataaaac gaattacctt atcgatttaa tgcatcgtca tcacctaaag
    16381 cgtccactca ttttactcaa ttggaataca ttgacgggtg atacatttat cacaaacggt
    16441 gaatatttta gaggtggtat catcattgag caattattaa aattaagttc taaagtagag
    16501 ggtatcgggt attggttgaa ttatgatttg cacgtcagtc attgtagaaa tgaacgggat
    16561 tatatgaatt ctattgaact gtttcatcaa tataatggaa aacgtccggt ctatttcacg
    16621 gcattgctat ttaataaatt aacaagcaat attttgtatt ctgatgatac ttgtattgtc
    16681 acgggaactg attcaaattt tcaaatattg ttatatgatg caaagcattt taatccgtac
    16741 ttagcgttgg acaatcaaat gaatatgcgt gcaacggaaa tgatccattt gaacattaat
    16801 gccctagaag acggtatgta taagattaaa cattttacct tagataaaga aaatggtgcg
    16861 ttatttaatc tttggcgcaa acatcatacg atacatggta tggacaagga ctctatagat
    16921 tacgttaatc gaatgagttt tccaaaatta gaagtgtacg atatagatgt gacggatacg
    16981 ctggcattaa atattaaaat gattacaaat ggaattcact taattgaagt aaaacgttac
    17041 ccaagttcat aaaatgatca caaatcacaa tttatgttat acataatttg tgattttttg
    17101 tattcaaagt taaaattgca aaaaattaat ggttaacatc tctgttgttg gcaatataaa
    17161 taatgattaa tcatttatga tgtaactaag gagatgaagg atatgaatca acaattaatt
    17221 gaagctttaa aatcaaaaga agacaaaatg attgagatca gacgttattt acatcagcat
    17281 ccagaattat cttttcatga ggatgaaacg gcgaaataca tcgccgaatt ttacaaaggt
    17341 aaagatgtag aagtagaaac gaatgtcgga ccacgtggaa ttaaagtaac gattgattca
    17401 gggaaacctg gtaaaacatt agcaatccgt gcagactttg atgcattacc cattactgaa
    17461 gatacaggat tatcttttgc atcacaaaat aaaggtgtta tgcacgcatg tggtcacgat
    17521 gcacatacag catacatgct tgtattagca gagacgcttg ctgaaatgaa agatagtttt
    17581 acaggaaaag tcgttgtgat acatcaacca gctgaagaag taccaccagg tggtgctaaa
    17641 gcaatgattg aaaatggtgt attagacggt gttgatcatg tattaggtgt acacgtcatg
    17701 agcacaatga aaacaggaaa agtgtattac agacctggtt atgttcaaac aggacgcgca
    17761 ttcttcaaat tgaaagttca aggtaaaggt ggccatggtt catcaccaca tatggccaat
    17821 gatgccattg ttgcaggtag ctacttcgtc acagcgttac aaacagttgt gtctagacga
    17881 ctaagtccat ttgaaacagg tgttgtcaca atcggttcat ttgacggtaa aggtcaattc
    17941 aatgtcatta aagatgttgt tgaaattgaa ggtgatgtac gtggattaac agatgctaca
    18001 aaagcaacaa ttgaaaaaga aattaaacgt ttatcaaaag gattagaagc attgtatggt
    18061 gtaacttgca cattagaata taacgatgat tatcctgcat tatataatga tccagagttt
    18121 actgagtacg tggctaagac gctaaaagaa gcagaccttg aatttggtgt cgaaatatgt
    18181 gaaccacaac caccttcaga agactttgca tactatgcta aggaacgacc aagtgccttt
    18241 atttatacag gtgcagctgt ggaagatggt gaaatttacc cacatcatca tcctaaattt
    18301 aacatttcag aaaaatcatt acttatttcg gcagaagctg tagggacagt tgttttagat
    18361 taccttaaag gagataacta acatgaatga aacgtatcgc gggggcaaca agttaatctt
    18421 aggtattgta ttaggtgtta ttacattttg gttgtttgca caatcacttg taaatgttgt
    18481 accaaattta caacaaagtt ttggtgcaga catgggaaca attagtattg cggtaagtct
    18541 aaccgcacta ttttcaggca tgtttgttgt tggagcaggc ggcctagcag ataaaattgg
    18601 acgcgtgaaa atgacgaata tcggcttatt attaagtatt attggttcag cattaattat
    18661 tattacaaat ttaccagcat tgctaatttt aggtcgtatt atacaaggcg tatcagcagc
    18721 gtgtattatg ccttccacat tggccattat gaaaacttat tatgagggtg ctgaacgtca
    18781 gcgtgcctta agctattggt ctatcggttc ttggggtgga agtggtatct gttcactctt
    18841 cggtggagca gttgcgacaa ctatgggttg gagatggatt ttcatcttct caattatcgt
    18901 tgccgtactt tcaatgttac tcatcaaagg aacgcctgaa acgaaatcag aagttaccaa
    18961 tacacataaa tttgacgttg caggtctaat tgttctagta gttatgttat taagtttaaa
    19021 cgttgttatt accaaaggtg cagcacttgg ttatacatca ttatggttct ttggtttgat
    19081 tgcggtcgtt gttgtagcat tctttatttt cttaaaagtt gagaaaaaag tagataatcc
    19141 gcttattgat tttaaattat ttgaaaataa accttataca ggtgcaacga tttcgaactt
    19201 cttattaaac ggtgttgcag gtacattaat tgtagcgaat acattcgtgc aacaaggttt
    19261 aggttataca gcattgcagg caggatactt atcaattact tatttaatca tggtgttatt
    19321 aatgattcga gttggtgaaa aattattaca aaaaatgggt tctaagcgac caatgttatt
    19381 aggtacattc attgtgatca ttggtattgc acttatttca ttagtattct taccaggcat
    19441 attttatgtt atcagttgtg tcgtaggata tttatgtttc ggactaggct taggtattta
    19501 tgcaacacct tctacagata cagctatttc aaatgcaccg ttagataaag ttggcgttgc
    19561 atcaggtatt tataaaatgg cttcatcact tggtggtgct ttcggtgtgg caattagtgg
    19621 tgcagtatat gctggtgcag ttgctgctac aagcattcat acaggtgcga tgattgcact
    19681 ttgggttaac gtattaatgg gtatcattgc atttatcgca atcttgtttg cgattcctaa
    19741 tgatgataaa cgtgccaaag atgcgaaata ataacgatgt tgcgtaactg tggtaactat
    19801 gacacaccca gtcttttaat aaatattaag atattaatag ggaatgatta aaataattag
    19861 actgctcgtt gttaagaggg cagtctttat ttatgtttaa atacttatga aagagcagtt
    19921 attggaacct ctttgagaat tttataagtt tttatagtat agaccctatg atttagggta
    19981 aatacgttat tagatagtca aatacttacg caagaaaaat catatatatt gaattgtaat
    20041 gggtaagttg atttctatta tttatgatga gtgtagctcc agattgctgc tcatcttttt
    20101 tataggaggt tatatttatg aaaaaaatcc tattaactgg ggcttcaggt tatattggta
    20161 gccatttaat ggaaaagtta aaagagaatc atgatattat tgcaatttca aggaatatag
    20221 ataataaaaa gaatgagaga aatgtaactt ggaaatcggc tgacttgttt gatttagctg
    20281 aaataaatga tgtaatggaa ggtattgata ctgccattta tcttgtccat tctatgatgc
    20341 cctcagccaa attaacacag gcaagctttg aggatatgga tgcaatatta gctgataatt
    20401 ttgctaaagc agcccgtcag aatggtgtga aacatattgt atttatgagt gggttgatac
    20461 caaatgatga tcaactatca ccacacttaa gaagtcgatt agaatgtgaa cgtattttag
    20521 ggtcatatgg tgtgcctgtg acaacattaa gagcaggttt aattatcggt tctaaaggta
    20581 gctcatatcc aatactgaaa acattagttg agcgtctacc agcattggta ctaccaaaat
    20641 gggcgtataa tcagacagta cctgttgcta ttgatgatgt cattcaaggt ttatataaag
    20701 tggtatcacg tagtcctaaa gtaaatgaag cgatagatat cggtggtccg ctacacatga
    20761 cttacaaaga tttatttaaa cagaccgcag aagtattaga taagcgttta ccaatgatag
    20821 atttacctat tattcctatt tggttaagta aattttgggt gaaactgatt tcaggtgttc
    20881 caaaagaaat ggtctatccc cttatggata gtttaattca taatatgaca cgtgatcaaa
    20941 agaggatagt agaaggtata tccataggtc aaatggatta taaagaaagt gtgcaacagg
    21001 cactagaaga ggcatcaacg tctaagaaga aacaaacaag cactataaag actgatgtca
    21061 aagatgttag agctatctcc agagtcgtat tgcctaagca tatgaatatg agtgcactag
    21121 cagaaatcta tgcgaatttc ttgaataaaa tcactttaaa tattgtaaac agtaattttg
    21181 atgaggaagc atttattatt tcattacctt tattaaagaa agaattactt ttattagaaa
    21241 aggatacaca aacttctgat gaagacagag tgtcatataa aattattggt ggcgattttg
    21301 cacttgccca taatggcggc aatgcacgat tagaatttag aaggctacct aatagtgatt
    21361 catgtattat cgcacttcaa gaatatgaac caacattgcc gtggtgggtc tataaatata
    21421 cacaagcaaa agttcataag agtgttatga atctctttaa gtttaattta tatatgaaac
    21481 gacataatca gaaacttagt agtgtgacta aacaaggact tatagtagga gctgttttag
    21541 gagcgctaat agtaaaaagt tacaaatcaa gaaattacta aattaatagt tatataaaat
    21601 atattaagac gacaattaat aaccatatga aaggagtaat ataatgacat ttaaacgttt
    21661 attgaaaacg atagttaaag tagtaacgcc tttattaggt ggtagattaa ttggtaagta
    21721 tgctgtccaa aatgcaagaa aagattataa agacaatgct aagccactat tgtcaccacc
    21781 aggatatata ttcccaattg tatggtcaat attatataca actattggta ttgcttatgc
    21841 aattataagt ggtaaatcaa ataatgcaaa agttaaaacg atttattatg cgcaattaag
    21901 ccttaactat ttatggtcta tcttatactt taaattcaaa ttacgttttg cagcattaat
    21961 tgaaagtttt atcctactta cagctgttat tatgatgact gtaaaatttt tcaaagttaa
    22021 acgaatagca ggtattatac ttattccata tatgttatgg agtacatttg cttcttattt
    22081 aactgttgga aactggctgt taaataaaga taatccacgt tataccgaac aaaaatagtg
    22141 aacgcaactg taaaaaatat agtgaagggg tcagactccg agaacagtgt ccgtcatgct
    22201 taccgcacac taataaaact ttattatata agtgtttata gccgatttaa gataacaaag
    22261 tttgctaaat ataaaagcca atccattgat gttggattgg ccttttacat gcatctgaat
    22321 ttctaatttt aaaaaaatat gaatataaat aagacagtaa aaattaaatt tcagttgttg
    22381 caatttcttc atctgtaggt acatcatcgt taagaccaac aagtgcttca gaaacatttc
    22441 gtgaatgata accgatacgt tcaagaacac caatcatatc gatatatagt aatccacctt
    22501 ttgttgtaca ttcaccacga ttaaggcgtt taatatgacc tttgcgtagt ttatgttcaa
    22561 tattaaatga ttctctacta cgttctacaa tttcatcttt tttcgttttg tcataaacat
    22621 ctaacatgtc gatggcttta tcaaatgact cagcaacatg gttgaataat ttatccatac
    22681 cgcgttgtgc gtcttctgta attcgaatat cttcatcatg ttgtcgtttt aactgagcga
    22741 catactcttc tgttagctct gctactttta aaatagagcg attgacatca aacataactg
    22801 ctaaacgctc aacgtctgcc ttcgtaatgg cttttgtaga aattctaact aaataatttc
    22861 gaatactatc attgattgtt tcaacagcct ggtgcttttg ttcaagcttt ttgatcaatt
    22921 ttttatcgtc ttttgtaatt tcgcgaatgt cttcaaacat tgataagaca atctgaccca
    22981 cattttgtaa ttctttttga gtttcttgta atgcaacacc aggtgcgtga taaacaagat
    23041 ctttgtttaa gtgctgaggt ttatagtcat cagcaatatc tttacctggg acaagctttg
    23101 taactatcca tgctaaccct gctacaaatg gtaattgaat caaagtattt gttatgttga
    23161 agataccatg tgatactgca atcgtcatcg ctggttttaa gtgccataca tcttgtaaca
    23221 aactaatcaa atgaatcaca actggcaaga aaattgtgaa gataattact ccgattaagt
    23281 taaagatgac gtgtacaagc gcagcacgtt ttgcagcgat tgagccggct aaactagcta
    23341 aaatagctgt aattgttgta ccgatgttat cgccaagtaa aactggaatt gctgcattta
    23401 aactaattaa atcttgttga taaaattctt gtaaaatacc aatcgtcgca cttgaacttt
    23461 gaacaagcgc tgttaatcct gcgccaacaa tgacagcgag tattggattt gatgacatat
    23521 caagcattaa ttgcttaaat ccatctaatg atgctaaagg tttaacggca tcacccataa
    23581 attctaaacc gaagaataga gaaccgaaac cgaatagtat acggccaatg ttattgattt
    23641 tagagcgttt aaagaaaaag attaaaaatg cacctaatgc taaaattggc attgcatatt
    23701 cgcctaaatc tataccgata ataaatgcag ttaccgttgt tccgatatta gcacccatta
    23761 tcactccaat ggcttgtttc aatgtcataa atccagctgt taccagtccg attgtgataa
    23821 ctgtcgtacc tgaactactt tgtattaaaa tagttacaac gatacctgca ataacaccta
    23881 atactggatt tgatgtaaat ttgtttaaaa tatctcgtag cctgtctcct gctgatgctt
    23941 gaagcccgtc tcccatgatt tttaagccgt aaaggaaaat acctaaacca cctaaaaagg
    24001 agaaaatgac ttctgtaacc gacatttcca ttatttcacc tcaaataagc tttatattta
    24061 gattatcgct tataattgta aatttaatgt taagattagg taaaattatt taacaatata
    24121 tgttatttgt atatgacttg taaaatatca tcactattta tgtaaatttt cagtgtgcaa
    24181 aggcaggttt gcaaacactt attttataaa atgatgcaat aaatcatgtg acaatatttt
    24241 ggcaataaaa aatcatgaga gtggaacaac gaaataattt ttataaaaac atcattttgt
    24301 cccagtctca tgattcgaaa tcaccaaata aaaatctatt aatggttttc gttataacaa
    24361 tttgtgttct tttaataatg tctcaatgta cgtacctttt acctttttaa ggaatccaga
    24421 taatgcgagt ttttgcattt tcgaatcttt agtaatctca cgcaaatctt ggtggtcatt
    24481 gagctcatat acagcatcca ttaagacgcg aagatcaaat ggactattga tgacctctgg
    24541 aacaccacga tctatattta gcaattgata aacagcttcc attgcagtac gaacagaata
    24601 ttctgttgta aatacagtgt cacgttctgt ttctgcaaag ttaccaataa atgctaaatt
    24661 ttgtgattga tgtgggacga ctaaaggtct gtcgccgata gcacgcgtca tgaaataaga
    24721 agtgatatat ggcatataaa caggaatcgt attagatgca tgttttgcta agtcttcaat
    24781 tttgtcagtt gatacaccta agtgatacag ccattcttga catatttcat taccactaca
    24841 ttctgtaatt ggctttttaa tataatcgcc gtttacatct gaatataagg cataaatcca
    24901 tgtagatatt tcattttcag gttggtcttt aaactgttgc tgacgattga ttgtaaaact
    24961 catttgccat gcagaatcat tgattgtaat aataccgcct gtaactgttt tgcctgcaag
    25021 tgggtcacgt ttacaaatac tttctattgt atcgataatt tctttattgt ttgttgtaga
    25081 agttgctgaa acaaaccaac ttttttcagg aatattttgg caaaacttgt caggattacc
    25141 aaattcagga ctttgtcgcg ctaaattttt ccataacgtc caactaccac ctaattcgtt
    25201 agttggtggc gcgggcgtat tgttatcacc atacctagag ctttccgtaa tactaccatt
    25261 tgtcacaaag acaagatcat ctacagtcag tttaattgac tctgctttgc catgtcggtg
    25321 aattaatatt tctcgggcaa ttttttgact tgtcgtaaca tctactttaa tatcttcgac
    25381 ttttacatcg tattcaaatt gaaccccatg cgatttcaaa tattcaacca taggtaatac
    25441 taaagattca tattgattat atttagtgaa ttttaaagct gaaaagtctg cgagaccacc
    25501 aatatgatga acgaatcgca ttagataacg acgcatttcc atagcagaat gccacggttc
    25561 aaatgcaaac attgttttcc agtaaatcca aaagtttgaa ttaaagaagt cgtcggaaaa
    25621 tacatccgtg attttgacat catctaaatc ttcttcattc gttaagcata aatctaaaat
    25681 ttctttaatt gcttttttgg tcaaagtgaa gtctccatcg gtaactaaac gttgaccctg
    25741 tttttcaata actcgacagc gagaatagtt agggtcttct ttgtttagcc aatagaactc
    25801 atctaataca gacgcgttat cgatttctaa tgaagggata gatctgaata agtcccacaa
    25861 acactcaaag tggttctcca tttcacgacc accgcggaca acatagcctt ttaaaggaat
    25921 attttctccg tcaagactac cacctgcttt aggtaactct tctaaaatat gaatcttcga
    25981 accttccatt tgaccatccc taattaagaa acaagctgcc gcaagtgaag ctaacccaga
    26041 cccgattaag taagcagatt tgttttctac attttcaggt tttttagggc gcgcaaatgc
    26101 ttcatagtta ccataactgt aatacatatt caccaagtcc tttcgtatta gaatacactc
    26161 aaactatacc ccttcgtgaa ttgcaaatat tagtatttat attggtaaat atgacgaaat
    26221 tagatcattt ttatattttt tatttttaaa attttatcac tcgaatgctt ttggattctg
    26281 aggtgcggga cttggaataa ttcgaatcat tattgtggca tgttaaacag ctaaatataa
    26341 gctatgcaca tttaacaagc ggatactcct gacgcaatta tattatcatt acatattgtt
    26401 taatcgtttg gggtaatgta attaaaaggt aaaataggca gagtgttata cataaaatgt
    26461 aaagaaagag gcgatagaat gtctaaaatc aggtctttta caatattaag tctacttatt
    26521 tacttagcta tgatgtgcta tacagtaatg aactattcaa aattaccaac gaaagtgcct
    26581 attcattaca atttagcagg ggatgctgat aactttgctg ataaatgggt gttgctactg
    26641 attaatggcg cttttatagt gatttggctt atatttttca tcgcaggtag atactatgaa
    26701 cgatttgcta aatggtcaca ttataatcac acaccacgtg aaattcagac gattaaatta
    26761 tttttaagta ctttaaactt ggagattatg agctatattt ctattttcat gattttagaa
    26821 atttggcaaa tacaacacaa tcatcaatta aatttattat ggtttaatat gatatttatc
    26881 attatcattg gtttgacgct tgtcatattt tgtttacttc ctacaattca taaaatgaga
    26941 gattctcaat aaaaatataa actgtcatga tgcagttttg aaacggattt cgaagattat
    27001 tataaataat atctagtatt tataattaaa atgatatatt atttattata gttaaatatt
    27061 gtgtatattt tgtgaacttt ttgtaaaaat tcgattgcct gtcacatata ggagtgttac
    27121 attttaaata tgtggtcatc gcaaaatata agttgaaata ggttgtagat taatcaggat
    27181 gataaatatt ttatataaag agagggagtc attatgacac tacttactgt aaatccattc
    27241 gataatgttg gattatcagc cttagttgca gcagtaccta ttattttatt tttattatgc
    27301 ttaaccgttt ttaaaatgaa aggcatttat gcagcattga caactttggt tgttacattg
    27361 attgtggctt tatttgtatt tgaattacca gcgcgtgtat cagcaggtgc gattacagaa
    27421 ggcgttgttg ccggtatttt cccaatagga tatatcgttt taatggcagt ttggttatat
    27481 aaagtttcta ttaaaacagg acaattttct attattcaag atagtattgc aagtatttca
    27541 gaggaccaaa gaatccaact attattaatt ggattttgtt tcaacgcatt tttagaaggt
    27601 gcagcaggat ttggtgtgcc aattgcgatt tgtgcagtat tattaattca acttggattt
    27661 gagccattaa aagcagcgat gttatgttta attgctaatg gtgcggcggg tgcctttggt
    27721 gcaattggtt taccagttag tattattgat acgtttaact taagtggagg cgttacaaca
    27781 ttagatgttg cgagatactc agcattaaca cttccaattt taaactttat tattccattt
    27841 gttttagtat tcattgtaga tggtatgaaa ggtattaaag aaattttacc tgtcatttta
    27901 ac
//
LOCUS       DAHHRR010000025        27452 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.025, whole genome shotgun sequence.
ACCESSION   DAHHRR010000025 DAHHRR010000000
VERSION     DAHHRR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27452
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.025"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            349..1686
                     /locus_tag="NBI42_000887"
     CDS             349..1686
                     /locus_tag="NBI42_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCD1777323.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDGYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRENIIEFLSYFQTSSPSYLIMASLESAAQFYKTYDSNMFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSIVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1688..2305
                     /locus_tag="NBI42_000888"
     CDS             1688..2305
                     /locus_tag="NBI42_000888"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCD1777324.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRTLNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRNQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2333..2662
                     /locus_tag="NBI42_000889"
     CDS             2333..2662
                     /locus_tag="NBI42_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCD1777325.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2876..3802
                     /locus_tag="NBI42_000890"
     CDS             2876..3802
                     /locus_tag="NBI42_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCD1777326.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQDMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIILTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3803..4606
                     /locus_tag="NBI42_000891"
     CDS             3803..4606
                     /locus_tag="NBI42_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCD1777327.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIDDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQYLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4623..4970
                     /gene="yabA"
                     /locus_tag="NBI42_000892"
     CDS             4623..4970
                     /gene="yabA"
                     /locus_tag="NBI42_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCD1777328.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5244..5969
                     /locus_tag="NBI42_000893"
     CDS             5244..5969
                     /locus_tag="NBI42_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCD1777329.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRADRLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            5962..6210
                     /locus_tag="NBI42_000894"
     CDS             5962..6210
                     /locus_tag="NBI42_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCD1777330.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            6212..7051
                     /gene="rsmI"
                     /locus_tag="NBI42_000895"
     CDS             6212..7051
                     /gene="rsmI"
                     /locus_tag="NBI42_000895"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCD1777331.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7336..9309
                     /gene="metG"
                     /locus_tag="NBI42_000896"
     CDS             7336..9309
                     /gene="metG"
                     /locus_tag="NBI42_000896"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCD1777332.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMINKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9341..10114
                     /locus_tag="NBI42_000897"
     CDS             9341..10114
                     /locus_tag="NBI42_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCD1777333.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10281..10817
                     /gene="rnmV"
                     /locus_tag="NBI42_000898"
     CDS             10281..10817
                     /gene="rnmV"
                     /locus_tag="NBI42_000898"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCD1777334.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRRDILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10828..11721
                     /gene="rsmA"
                     /locus_tag="NBI42_000899"
     CDS             10828..11721
                     /gene="rsmA"
                     /locus_tag="NBI42_000899"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCD1777335.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDSRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11821..12084
                     /locus_tag="NBI42_000900"
     CDS             11821..12084
                     /locus_tag="NBI42_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCD1777336.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12394..13242
                     /locus_tag="NBI42_000901"
     CDS             12394..13242
                     /locus_tag="NBI42_000901"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCD1777337.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFNIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            13256..14080
                     /gene="purR"
                     /locus_tag="NBI42_000902"
     CDS             13256..14080
                     /gene="purR"
                     /locus_tag="NBI42_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCD1777338.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            14097..14477
                     /locus_tag="NBI42_000903"
     CDS             14097..14477
                     /locus_tag="NBI42_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCD1777339.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNVDGKIVSAD
                     VQAQTKQVLENLKIVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPRDVKVEIELVSKIKEL"
     gene            14553..14855
                     /gene="spoVG"
                     /locus_tag="NBI42_000904"
     CDS             14553..14855
                     /gene="spoVG"
                     /locus_tag="NBI42_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCD1777340.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            15042..15172
                     /locus_tag="NBI42_000905"
                     /pseudo
     CDS             15042..15172
                     /locus_tag="NBI42_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15195..16547
                     /gene="glmU"
                     /locus_tag="NBI42_000906"
     CDS             15195..16547
                     /gene="glmU"
                     /locus_tag="NBI42_000906"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCD1777341.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTFIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            16694..17659
                     /locus_tag="NBI42_000907"
     CDS             16694..17659
                     /locus_tag="NBI42_000907"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCD1777342.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            17809..18462
                     /locus_tag="NBI42_000908"
     CDS             17809..18462
                     /locus_tag="NBI42_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCD1777343.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            18773..19345
                     /locus_tag="NBI42_000909"
     CDS             18773..19345
                     /locus_tag="NBI42_000909"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCD1777344.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            19345..22851
                     /gene="mfd"
                     /locus_tag="NBI42_000910"
     CDS             19345..22851
                     /gene="mfd"
                     /locus_tag="NBI42_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCD1777345.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEDLYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNIEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIDYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIEEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            22841..24367
                     /locus_tag="NBI42_000911"
     CDS             22841..24367
                     /locus_tag="NBI42_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD1777346.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDSHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNKVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTKKYHLHAMRRFFMNVVFGMVFMSIVVQLVL
                     FIVTTHGRITGLIELLCAAFLGIAALFYYIFKFNVLTYKELTYLPFGSKLYQMKKGRR
                     "
     gene            24367..25560
                     /locus_tag="NBI42_000912"
     CDS             24367..25560
                     /locus_tag="NBI42_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCD1777347.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYETHDQFEDVYNDIVAQLVETANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKITTPTYLYHDFDFAAEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            25557..25820
                     /locus_tag="NBI42_000913"
     CDS             25557..25820
                     /locus_tag="NBI42_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCD1777348.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            25838..26230
                     /locus_tag="NBI42_000914"
     CDS             25838..26230
                     /locus_tag="NBI42_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCD1777349.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            26335..26736
                     /locus_tag="NBI42_000915"
     CDS             26335..26736
                     /locus_tag="NBI42_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCD1777350.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDAKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            26916..>27452
                     /gene="tilS"
                     /locus_tag="NBI42_000916"
     CDS             26916..>27452
                     /gene="tilS"
                     /locus_tag="NBI42_000916"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCD1777351.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFE"
BASE COUNT         9899 a         3696 c         5497 g         8360 t
ORIGIN      
        1 caaaaaaagg atgcgatgag ccgcattgag ttttggaaat gataatttaa taatgtgcac
       61 ttttgattgt ttaaatctgt gactagaagt ataagtgaag acattcagaa gtattataaa
      121 aagtgaacag cagtaagata gtttttaatc ataaatcatc ttactgctgt ttttaggttt
      181 tatgtctaat atcttttata tcgaaataca aaaaggaaat taattattat acaatagaca
      241 agctattgca taagtaacac taacttttgt caaagaagtg ttactatata attaatactt
      301 ttgaaagtaa ctaattccaa aacagtgtaa ataaaggaag cgtatatcat gaagcaacct
      361 attttaaata aattagaaag tttaaatcaa gaagaagcga tttctttgca tgttccgggt
      421 cataaaaata tgactatcgg tcatttatct caattatcaa tgacaatgga taaaactgaa
      481 atacctggat tagatgattt acatcatcct gaagaagtca ttttggaaag tatgaagcag
      541 gtggagaaac attcagatta tgatggttat ttcttagtga atggcaccac ttcgggaata
      601 ttatctgtca tccagtcttt ttcacagaaa aaaggcgata tcttaatggc aagaaatgta
      661 cataaatctg tattacatgc gctcgatatt agtcagcaag aagggcattt tattgaaacg
      721 catcaaagtc cgttaacgaa tcattataat aaagttaatt taagccgttt gaataatgac
      781 ggtcacaaac ttgctgtgtt gacttatcct aactattatg gtgaaacatt taatgtagaa
      841 gaggttatca aatctttgca ccaattaaat attcctgtac tcattgacga agcacacggc
      901 gcgcactttg gattgcaagg atttccagat tctacattaa attatcaagc tgactatgtt
      961 gttcaatctt ttcataaaac gttaccagct ttaacgatgg gctcggtact ttatattcat
     1021 aaaaatgcac cttatagaga aaatattata gaatttctaa gctatttcca aacatctagt
     1081 ccttcatatt tgattatggc tagtttagag tcagctgccc agttctataa aacatatgat
     1141 agtaacatgt tttttgataa gagagcgcaa ttaatcgaat gtttggagaa gaaaggtttt
     1201 gaaatgcttc aagttgatga tccgttgaag ttgttgataa aatatgaagg gtttacaggt
     1261 catgatattc aaaattggtt tatgaatgca catatctatt tagaattagc tgacgactat
     1321 caagcattag cgatattgcc gttatggcat catgatgata cgtatttatt tgattcgctt
     1381 ttacgtaaaa ttgaagatat gattttaccg aaaaaatcag tttctatagt taaacaaaca
     1441 caacttttaa caactgaagg taactataaa ccaaaacgct ttgaatatgt tacttggtgt
     1501 gatttgaaaa aggcaaaagg taaagttctg gcgcgacata ttgtcccata tccgccaggg
     1561 attcctatta ttttcaaagg agaaacaata actgaaaata tgatagaatt ggtaaatgaa
     1621 tatctggaaa ctggaatgat agttgaagga attaaaaata ataaaatttt agttgaggat
     1681 gaataaaatg tcagctttta taacttttga gggcccagaa ggctcaggta aaacaactgt
     1741 aattaatgaa gtttatcata gattagtaaa agattatgat gtcattatga ctagagaacc
     1801 aggtggtgtt cctactggtg aagaaatacg taaaattgta ttagaaggca atgatatgga
     1861 cattagaact gaagcaatgt tatttgctgc atctagaaga gaacatcttg tattaaaggt
     1921 cataccagct ttaaaagaag gtaaggttgt gttgtgtgat cgctatatcg atagttcatt
     1981 agcttatcaa ggttatgcta gagggattgg cgttgaagaa gtaagaacat taaacgaatt
     2041 tgcaataaat ggattatatc cagacttgac gatttattta aatgttagtg ctgaagtagg
     2101 tcgcgaacgt attattaaaa attcaagaaa tcaaaataga ttagatcaag aagatttaaa
     2161 gtttcacgaa aaagtaattg aaggatacca agaaatcatt cataatgaat cacaacggtt
     2221 caaaagcgtt aatgcagatc aacctcttga aaatgttgtt gaagacacgt atcaaactat
     2281 catcaaatat ttagaaaaga tatgatataa ttgttagaag aggtgttata aaatgaaaat
     2341 gattatagcg atcgtacaag atcaagatag tcaggaactt gcagatcaac ttgttaaaaa
     2401 taactttaga gcaacaaaat tggcaacaac aggtgggttt ttaagagcag gtaatacaac
     2461 attcttatgt ggtgtcaatg atgaccgagt agatgaaata ttgtctgtga ttaatcaaac
     2521 gtgtggtaat agagaacagt tggtttcacc tattacacct atgggaggca gtgcggattc
     2581 gtacattcca tatccagttg aagttgaagt tggcggtgct actgtatttg ttatgccagt
     2641 tgatgcattc catcaatttt aattctataa tacaatcatc aattagagat acttaaaata
     2701 gtgtattaat aagttattaa caattttggg ttgcttgact gcgactagtt cagatgccaa
     2761 tagatttgat ttttgtggtt ctaagaataa tcacaaatca tggtcgctat tgttgcagta
     2821 attagttgct ctttggcaac ctttttatat aaaagcaaaa gggagtttgt aatgtatgga
     2881 tgaacagcaa caattgacga atgcatatca ttcaaataaa ttatcgcatg cctatttatt
     2941 tgaaggtgat gatgcacaaa cgatgaaaca agttgcgatt aattttgcaa agcttatttt
     3001 atgtcaaaca gatagtcaat gtgaaacaaa ggttagtaca tataatcatc cagactttat
     3061 gtatatatca acaactgaga atgcaattaa gaaagaacaa gttgaacaac ttgtgcgtca
     3121 tatgaatcaa cttcctatag aaagcacaaa taaagtgtac atcattgaag actttgaaaa
     3181 gttaactgtt caaggggaaa acagtatctt gaaatttctt gaagaaccac cggacaatac
     3241 gattgctatt ttattgtcta caaaacctga gcaaatttta gacacaatcc attcaaggtg
     3301 tcagcatgta tatttcaagc ctattgataa agaaaagttt ataaatagat tagttgaaca
     3361 agacatgtct aagccagtag ctgaaatgat tagtacttat actacgcaaa tagataatgc
     3421 aatggcttta aatgaagaat ttgatttatt agcattaagg aaatcagtta tacgttggtg
     3481 tgaattgttg cttactaata agccaatggc acttataggt attattgatt tattgaaaca
     3541 ggctaaaaat aaaaaactgc aatctttaac tattgcagct gtgaatggtt tcttcgaaga
     3601 tatcatactt acaaaggtaa atgtagagga taaacaaata tatagtgatt taaaaaatga
     3661 tattgatcaa tatgcgcaaa agttgtcgtt taatcaatta attttgatgt ttgatcaact
     3721 gacggaagca cataagaaat tgaatcaaaa tgtaaatcca acgcttgtat ttgaacaaat
     3781 cgtaattaag ggtgtgagtt agatgccaaa tgtaataggt gttcagtttc aaaaagcggg
     3841 aaaattagaa tattatacac ctaatgatat acaagtagat atagatgact gggtagttgt
     3901 cgaatctaaa agaggcatag agataggtat tgttaaaaat ccattaatgg atattgctga
     3961 agaggatgtt gtgttacctc ttaaaaatat tattcgcatt gctgatgaca aagatattga
     4021 taaatttaat tgtaatgaac gagatgctga aaatgcatta atactatgta aagacattgt
     4081 aagagaacaa ggtttggaca tgcgtttagt caattgcgaa tatacattag ataaatcgaa
     4141 agttattttt aattttacgg cggatgatcg tattgatttt agaaaattag taaaaatatt
     4201 agcgcaatat ttaaaaacac gtatcgagtt gagacaaatt ggtgtaaggg atgaagccaa
     4261 attgcttggg ggtatcggac cttgtggtag gtcgttatgt tgttctacat ttttaggaga
     4321 ttttgaacca gtatcgatta agatggctaa ggatcaaaat ttatcattaa atccaactaa
     4381 aatttccggt gcatgtggtc gtttgatgtg ttgtttaaaa tatgaaaatg actactatga
     4441 ggaagtacgt gcgcaattac ctgatatcgg tgaagcaatt gaaacgcctg atggtaacgg
     4501 gaaagtagtt gctttaaata tattagacat ttctatgcag gtgaagcttg agggacatga
     4561 acagccactt gaatataaat tagaagaaat agaaactatg cattaaggag gcattactac
     4621 atttggatcg caatgaaata tttgaaaaaa taatgcgttt agaaatgaat gtcaatcaac
     4681 tttcaaagga aacttcagag ttaaaggcac ttgcagttga attagtagaa gaaaatgtag
     4741 cgcttcaact tgaaaatgat aatttgaaaa aggtgttggg caatgatgaa ccaactacta
     4801 ttgatactgc gaattcaaaa ccagcaaaag ctgtgaaaaa gccattacca agtaaagata
     4861 atttggctat attgtatgga gaaggatttc atatttgtaa aggcgaatta tttggaaaac
     4921 atcgacatgg tgaagattgt ctgttctgtt tagaagtttt aagtgattaa tcaagcacac
     4981 tcaaatagtg ttataattat aaatgaatat ggtttggata agtctgagac aatgcatgtt
     5041 tcaggcttta attgtgtata acgttttggt gattgcataa gagatagcgg tactaaatgt
     5101 tattattaac tgtgcacgca gtatcattag ttataaaatg tagctgttaa aagtcaaaaa
     5161 tacatcgaat gtagttaggc atataatata aaaagagatt tcaattactc aatagaaaaa
     5221 ggttgtcttc ataggagtta aaaatgttaa aagagaatga acgatttgat caactaatca
     5281 aagaagattt tagtattatt caaaatgatg atgttttttc gttttcaacg gatgctttgt
     5341 tgttagggca ttttacaaaa cctagaacaa aagatattgt gttggactta tgttcaggca
     5401 atggggtgat acccttgtta ttgtttgcga aacatccacg acatatagaa ggtgttgaga
     5461 ttcaaaaaac acttgtcgat atggcgcgac gcacatttca attcaatgat gttgatgaat
     5521 atctaacaat gcatcacatg gatttgaaaa acgttactaa agtatttaaa ccttcacaat
     5581 atactttagt aacgtgtaat ccgccttatt ttaaagagaa tcagcaacac caacatcaaa
     5641 aagaagcaca taagatagcg agacatgaga ttatgtgtac acttgaagat tgcatgattg
     5701 cagcccgtca tttattaaaa gaaggtggca ggctaaacat ggtacatcgt gcagatagac
     5761 taatggatgt cttgtttgaa atgagaaaag tgaatattga acctaagaaa gtcgttttta
     5821 tatatagtaa agtagggaaa tcggcacaaa cgatagttgt agaaggtcga aaaggtggaa
     5881 atcaaggttt agaaatcatg cccccatttt atatttataa tgaagatggt aattatagcg
     5941 aagaaatgaa ggaagtatat tatggatagt cattttgtat atattgtaaa atgtagtgat
     6001 ggaagtttat atacaggata cgctaaagac gttaatgcac gtgttgaaaa acataaccga
     6061 ggtcaaggag ccaaatatac gaaagtaaga cgtccggtgc atttagttta tcaagaaatg
     6121 tatgagacaa agtctgaagc attgaagcgt gaatatgaaa ttaaaactta taccagacaa
     6181 aagaaattgc gattaattaa ggagcgatag tatggctgta ttatatttag tgggcacacc
     6241 aattggtaat ttagcagata ttacttatag agcagttgat gtattgaaac gtgttgatat
     6301 gattgcttgt gaagacacta gagtaactag taagctgtgt aatcattatg atattccaac
     6361 tccattaaag tcatatcacg aacataacaa ggataagcag actgctttta tcattgaaca
     6421 gttagaatta ggtcttgacg ttgcgctcgt atctgatgct ggattgccct taattagtga
     6481 tcctggatac gaattagtag tagcagccag agaagctaat attaaagtag agactgtgcc
     6541 tggacctaat gctgggctga cggctttgat ggctagtgga ttaccttcat atgtatatac
     6601 atttttagga tttttgccac gaaaagagaa agaaaaaagt gctgtattag agcaacgtat
     6661 gcatgaaaat agcacattaa ttatatacga atcaccgcat cgtgtgacag atacattaaa
     6721 aacaattgca aagatagatg caacacgaca agtatcacta gggcgtgaat tgactaagaa
     6781 gttcgaacaa attgtaactg atgatgtaac acaattacaa gcattgattc agcaaggcga
     6841 tgtgccattg aaaggcgaat tcgttatctt gattgagggt gctaaagcga acaatgagat
     6901 atcgtggttt gatgatttat ctatcaatga gcatgttgat cattatattc aaacttcaca
     6961 gatgaaacca aaacaagcta ttaaaaaagt tgctgaagaa cgacaactta aaacgaatga
     7021 agtatataat atttatcatc aaataagtta atcactttat cgattatatg aaattttaaa
     7081 cgattttata aacgtatgct gtaattttaa atggtaagtt atcattttgt attgatactg
     7141 ataaaatgat gttgacttag ataaaaaaat gatgacatcg acgtttttta atgtaaaata
     7201 aatacattga aagtaataaa taccttaaca ttgaataaga tgaaaatgag atgacgagat
     7261 aaatgttcgc gtccgttgaa atgcatagaa atcttagata ttatttgaag tgagacatta
     7321 cgaggaggaa cagttatggc taaagaaaca ttttatataa caaccccaat atactatcct
     7381 agtgggaatt tacatatagg acatgcatat tctacagtgg ctggagatgt tattgcaaga
     7441 tataagagaa tgcaaggata tgatgttcgt tatttgactg gaacggatga acacggtcaa
     7501 aaaattcaag aaaaagctca aaaagctggt aagacggaaa ttgaatattt ggatgagatg
     7561 attgctggaa ttaaacaatt gtgggctaag cttgaaattt caaatgatga ttttatcaga
     7621 acaactgaag aacgtcataa acatgtcgtt gagcaagtat ttgaacgttt attaaagcaa
     7681 ggtgatatct atttaggtga atatgaaggt tggtattctg ttccggatga aacatactac
     7741 acagagtcac aattagtaga cccacaatac gaaaacggta aaattattgg tggtaaaagt
     7801 ccagattctg gacatgaagt tgaactagta aaagaagaaa gttatttctt taatattagt
     7861 aaatatacag accgtttatt agagttctat gaccaaaatc cagattttat acaaccacca
     7921 tcaagaaaaa atgaaatgat taacaacttc attaaaccag gacttgctga tttggctgtt
     7981 tctcgtacat catttaactg gggtgtccat gttccgtcta atccaaaaca tgttgtttat
     8041 gtttggattg atgcgttagt taactatatt tcagcattag gctatttatc agatgatgag
     8101 tcactattta acaaatactg gccagcagat attcatttaa tggctaagga aattgtgcga
     8161 ttccactcaa ttatttggcc tattttattg atggcattag acttaccgtt acctaaaaaa
     8221 gtctttgcac atggttggat tttgatgaaa gatggaaaaa tgagtaaatc taaaggtaat
     8281 gtcgtagacc ctaatatttt aattgatcgc tatggtttag atgccacacg ttattatcta
     8341 atgcgtgaat taccatttgg ttcagatggc gtatttacac ctgaagcatt tgttgagcgt
     8401 acaaatttcg atctagcaaa tgacttaggc aacttagtaa accgtacgat ttctatgatt
     8461 aataagtact ttgatggcga attaccagcg tatcaaggtc cacttcatga attagatgaa
     8521 gaaatggaag ctatggcttt agaaacagtg aaaagctata ctgaaagcat ggaaagcttg
     8581 caattttctg tggcattatc tacggtatgg aagtttatta gtagaacgaa taagtatatt
     8641 gacgaaacaa cgccttgggt attagctaag gatgatagcc aaaaagatat gttaggcaat
     8701 gttatggctc acttagttga aaatattcgt tatgcagctg tattattacg tccattctta
     8761 acacatgcgc cgaaagagat ttttgaacaa ttgaacatta acaatcctca atttatggaa
     8821 tttagtagtt tagagcaata tggtgtgctt actgagtcaa ttatggttac tgggcaacct
     8881 aaacctattt tcccaagatt ggatagcgaa gcggaaattg catatatcaa agaatcaatg
     8941 caaccgcctg ctactgaaga ggaaaaagaa gagattccta gcaaacctca aattgatatt
     9001 aaagactttg ataaagttga aattaaggca gcaacgatta ttgatgctga acatgttaag
     9061 aagtcagata agcttttaaa aattcaagta gacttagatt ctgaacaaag acaaattgta
     9121 tcaggaattg ccaaattcta tacaccagat gatattattg gtaaaaaagt agcagttgtt
     9181 actaacttga aaccagctaa attaatggga caaaaatctg aaggtatgat attatctgct
     9241 gaaaaagatg gtgtattaac cttagtaagt ttaccaagtg caattccaaa tggtgcagtg
     9301 attaaataac tgtatttttt aaaaattagg agagataatt atgttaatcg atacacatgt
     9361 ccatttaaat gatgagcaat acgatgatga tttgagtgaa gtgattacac gtgctagaga
     9421 agcaggtgtt gatcgtatgt ttgtagttgg ttttaacaaa tcgacaattg aacgcgcgat
     9481 gaaattaatc gatgagtatg attttttata tggcattatc ggttggcatc cagttgacgc
     9541 aattgatttt acagaagaac acttggaatg gattgaatct ttagctcagc atccaaaagt
     9601 gattggtatt ggagaaatgg gattagatta tcactgggat aaatctcctg cagatgttca
     9661 aaaggaagtt tttagaaagc aaattgcttt agctaagcgt ttgaagttac caattatcat
     9721 tcataaccgt gaagcaacac aagactgtat cgatatctta ttggaggagc atgctgaaga
     9781 ggtaggcggg attatgcata gctttagtgg ttctccagaa attgcagata ttgtaactaa
     9841 taagctgaat ttttatattt cattaggtgg acctgtgaca tttaaaaatg ctaaacagcc
     9901 taaagaagtt gctaagcatg tgtcaatgga gcgattgcta gttgaaaccg atgcaccgta
     9961 tctttcgcca catccgtata gaggaaagcg aaatgaaccg gcgagagtaa ctttagtagc
    10021 tgaacaaatt gctgaattaa aaggcttatc ttatgaagaa gtgtgcgaac aaacaactaa
    10081 aaatgcagag aaattgttta atttaaattc ataaagttaa aagtgaggaa gatcaccgcc
    10141 ataaatgtaa acgatgctat attcgtttaa tatgctatgg ttctttctca cttttttaaa
    10201 ttaaaatatc gtgcatgtgc aatacatgcg atagagatgg ttagtgcttt gaaattaaga
    10261 attgtaggaa ggcgttttaa atgaaaatca atgagtttat agttgtagaa ggacgagatg
    10321 atactgagcg tgttaaacga gctgttgaat gtgatacgat tgaaacgaat ggtagtgcca
    10381 tcaacgaaca aactttagaa gtaattagaa atgctcaaca aagtagaggc gttattgtat
    10441 taacagatcc agatttccca ggagataaaa ttagaagtac aattactgaa catgtcaaag
    10501 gtgttaaaca tgcgtatatt gatagagaaa aagctaaaaa taaaaaaggg aaaattggtg
    10561 ttgaacatgc cgacttaatt gatattaaag aagcgttaat gcatgttagt tcaccctttg
    10621 atgaagctta tgaatcaatt gataaatctg tgcttataga gttggggtta atcgtaggga
    10681 aagatgcaag gcgccgtaga gacattttaa gtagaaaatt gcgaatcggc cattccaatg
    10741 gtaagcagtt attgaaaaag ttaaatgcat ttggttatac cgaagcggat gtaaggcaag
    10801 ctttagaaga tgaatgagga agtgaaaatg ttggataata aagatattgc aacaccatca
    10861 agaacgcgag cgttgttaga taaatatggc tttaatttta aaaaaagttt aggacagaac
    10921 tttttaatag atgtgaatat cattaataat atcattgatg caagtgatat tgatgcacaa
    10981 actggtgtga ttgaaattgg tccaggtatg gggtcgttga cagaacaatt ggccagacat
    11041 gctaaaagag tattggcgtt tgaaattgat caacgtttaa tacctgtatt aaatgataca
    11101 ttatcacctt atgataatgt gacggtgatt aatgaagata ttttaaaagc gaatattaaa
    11161 gaagctgttg aaaatcattt acaagattgc gaaaaaataa tggttgttgc aaacctgccg
    11221 tactatatta cgacgccaat tttattaaat ttaatgcaac aagatatacc aattgatggc
    11281 tacgtggtga tgatgcaaaa agaagtgggc gaacgcttaa atgctgaagt aggttcaaaa
    11341 gcatatggtt cattatcaat tgtcgtacaa tactatacag agactagtaa agtattaacg
    11401 gtacctaaat ctgtatttat gccaccacct aatgttgatt caatagttgt aaaactgatg
    11461 cagagaactg aaccgttagt aacagtagat aacgaggaag cattctttaa gttagcaaaa
    11521 gcagcatttg cacaaagaag aaagacaatt aacaataact atcaaaatta ttttaaagat
    11581 ggtaaacaac acaaagaagt gattttacaa tggttagaac aagcgggtat tgattcaaga
    11641 cgtcgcggtg aaacgctatc tattcaagat tttgctaaat tgtatgaaga aaagaaaaaa
    11701 ttccctcaat tagaaaatta aatgattgac aaagtaaagc actattgtta aaatttaaat
    11761 tttgtttgac gaaaacgctg caaatatggt attatgtaac ttgtagcgag gtggagcaat
    11821 atgccaaaat caattttgga catcaaaaat tctattgatt gtcatgtagg aaatcgtatt
    11881 gtactgaaag ccaatggagg ccgtaagaaa acaataaaac gttctggaat tttaaaagaa
    11941 acatatccgt cagttttcat tgttgagtta gatcaagaca aacacaactt tgagagagta
    12001 tcttatacat acactgatgt gttaactgaa aatgttcaag tttcatttga agaggataat
    12061 catcacgaat caattgcaca ctaaataaga catatagaga tgttagacgt ttcttagtat
    12121 aagaagtaaa tattatgata attatttgag tgttgggcat tatgttcaat actcttttta
    12181 tttacaaaat gtttaacact gatgtttcgc ttatagattt ttcagtaaat ggataattgt
    12241 atttataaac acaaatacaa gtaaatacta agtaattaga tggagaaaat tactttttta
    12301 ttaaaaaaac actaaaaaac aaattaaaat gtcaaatatt aattctcttt atgttaaaat
    12361 catcatatta agataacgaa aagagggcgg aaaatgatat atgaaacggc accagccaaa
    12421 attaatttta cgctcgatac actttttaaa agaaatgatg gctatcatga gattgaaatg
    12481 ataatgacaa cagttgattt aaatgatcgt ttaacttttc ataaaagaaa agatcgaaag
    12541 atagttgttg agattgaaca taattatgtg ccttctaatc ataaaaatct cgcatatcgt
    12601 gcagcgcaac tatttattga gcaatatcaa ctaaagcaag gtgtaacaat ttctatcgat
    12661 aaagaaatac ctgtttctgc tggcttagct ggaggttcgg ctgatgcagc agcaacgtta
    12721 agaggattga atcgactttt taatataggg gcgagtttgg aagaattggc tctactaggc
    12781 agtaaaatcg ggacagatat tccgttttgt atttataata aaactgcact atgtactgga
    12841 agaggagaga aaatcgagtt tttaaataaa ccaccttcag cttgggtgat tcttgctaaa
    12901 ccaaacttag gcatatcatc accagatata tttaagttga ttaatttaga taagcgttac
    12961 gacgtacata cgaaaatgtg ttatgaggcc ttagaaaatc gagattatca acaattatgt
    13021 caaagtttgt ctaatcgatt agagccaatt tctgtttcaa aacacccaca aatcgataaa
    13081 ttaaaaaata atatgttgaa aagtggtgca gatggtgcgt taatgagtgg aagcggaccg
    13141 actgtgtatg ggctagcacg aaaagaaagc caagcaaaaa atatttataa tgcagttaac
    13201 ggttgttgta atgaagtgta cttagttaga ctattaggat agaagggttg aaaagatgag
    13261 atataaacga agcgagagaa ttgtttttat gacgcaatat ttgatgaacc atccgaataa
    13321 attgattcca ttaacttttt ttgtgaaaaa atttaaacag gcgaagtctt caataagtga
    13381 agatgtccaa attataaaaa atacattcca aaaagaaaag ttaggtacag taattactac
    13441 tgctggcgca agtggtggtg ttacgtataa accaatgatg agtaaagaag aggcgactga
    13501 agttgttaat gaggtcatta ctctattaga agagaaagaa cgtttgttac ctggcgggta
    13561 tttattttta tcagatttgg taggtaatcc atcgctacta aacaaagttg gtaagttaat
    13621 tgccagtatt tacatggaag aaaaattaga tgctgttgtt accattgcga caaaaggtat
    13681 ttcattggca aatgcggttg ctaatatttt aaatttacca gtagtagtga ttagaaaaga
    13741 caacaaggtg actgaaggtt ctacagtttc aattaattac gtttcaggat cttcaagaaa
    13801 aatagagaca atggtacttt cgaagagaac tttagcagaa aattcaaatg ttttagttgt
    13861 cgatgatttt atgagggctg gtggctctat taatggtgtt atgaatttaa tgaatgagtt
    13921 taaagcccat gtaaaagggg tatcagtact tgtagaatca aaagaagtta aacaaagatt
    13981 gattgaagat tatacttcct tagtgaaatt atctgatgta gatgaatata atcaagagtt
    14041 taacgtagaa cctggcaaca gtttatctaa gttttcataa aaggagtttt agtattatga
    14101 aaatcattaa cacaacaaga ttaccggaag cacttggacc atattcgcat gcaacagttg
    14161 taaatggtat ggtttatact tctggtcaga ttccattgaa tgttgatggg aaaatcgtaa
    14221 gcgctgatgt tcaagcacag acaaaacaag ttttagaaaa tttaaagatt gttttggaag
    14281 aagcaggatc tgatttgaat tctgttgcga aagcgaccat tttcattaaa gatatgaatg
    14341 atttccaaaa aataaatgaa gtgtatggtc aatattttaa tgaacacaag ccagcgcgta
    14401 gttgtgtaga ggttgcgcgt ttgccaagag atgtgaaagt agaaattgaa ttagtaagta
    14461 aaattaagga attataattt tcgattaata tgtttaatca agcttctaaa taaaacagat
    14521 atatatatat atactatagg ggggctcact acatgaaagt gacagatgta agacttagaa
    14581 aaatacaaac agatggacga atgaaagcac tcgtttccat tacattagat gaagctttcg
    14641 taattcatga tttacgtgta attgaaggaa actctggctt gttcgttgca atgccaagta
    14701 aacgtacacc agatggtgaa ttccgcgaca tcgcgcatcc tattaattca gatatgagac
    14761 aagaaattca agatgcagtg atgaaagtat atgatgaaac agatgaagta gtacctgata
    14821 aaaacgctac atcagaagat tcagaagaag cttaatcaat tttatattta gcgatgtaat
    14881 acatttgcaa taagttgatt tgatactgtc gataaagcat aaagctttgt cggcagtttt
    14941 ttagtttgta ttaatatttt tttatgaaat gaaaggctaa taaatatata cgttaacaga
    15001 ttatgatgat atgaaaatta ttgattcatt gctgataaaa attgcgtttg aatgatgctc
    15061 gtatttttga agtaagaaaa agttgttttt aaaattacaa cgaattaaaa acaatgcctt
    15121 ttatatgttg aaagagtatt gcagattaaa ttataataat gacgaagtgt aaaatttaat
    15181 gggggttaat gttcatgcga agacacgcga taattttggc agcaggtaaa ggcacaagaa
    15241 tgaaatctaa aaagtataaa gtgctacacg aggttgctgg gaaacctatg gtcgaacatg
    15301 tattggaaag tgtgaaaggc tctggtgtcg atcaagttgt aaccatcgta ggacatggtg
    15361 ctgaaagtgt aaaaggacat ttaggcgagc gttctttata cagttttcaa gaggaacaac
    15421 tcggtactgc gcatgcagtg caaatggcga aatcacactt agaagacaag gaaggtacga
    15481 cgatcgttgt atgtggtgat acaccgctca tcacaaagga aacattagaa acattgattg
    15541 cgcatcacga ggatgctaat gctcaagcaa ctgtattatc tgcatcgatt caacaaccat
    15601 atggatacgg aagaatcgtt cgaaatgcgt caggtcgttt agaacgcata gttgaagaga
    15661 aagatgcaac gcaagctgaa aaggatatta atgaaattag ttcaggtatt tttgcgttta
    15721 ataataaaac gttgtttgaa aaattaacac aagtgaaaaa tgataatgcg caaggtgaat
    15781 attacctccc tgatgtattg tcgttaattt taaatgatgg cggcatcgta gaagtctatc
    15841 gtaccaatga tgttgaagaa atcatgggtg taaatgatcg tgtaatgctt agtcaggctg
    15901 agaaggcgat gcaacgtcgt acgaatcatt atcacatgct aaatggtgtg acaatcatcg
    15961 atcctgacag cacttttatt ggtccagacg ttacaattgg tagtgataca gtcattgaac
    16021 caggcgtacg aattaacggt cgtacagaaa ttggtgaaga tgttgttatt ggtcagtact
    16081 ctgaaattaa caatagtacg attgaaaatg gtgcatgtat tcaacagtct gttgttaatg
    16141 atgctagcgt aggagcgaat actaaggtcg gaccgtttgc gcaattgaga ccaggcgcgc
    16201 aattaggtgc agatgttaag gttggaaatt ttgtagaaat taaaaaagca gatcttaaag
    16261 atggtgccaa ggtttcacat ttaagttata ttggcgatgc tgtaattggc gaacgtacta
    16321 atattggttg cggaacgatt acagttaact atgatggtga aaataaattt aaaactatcg
    16381 tcggcaaaga ttcatttgta ggttgcaatg ttaatttagt agcacctgta acaattggtg
    16441 atgatgtatt ggtggcagct ggttccacaa tcacagatga cgtaccaaat gacagtttag
    16501 ctgtggcaag agcaagacaa acaacaaaag aaggatatag gaaataatca tttacgtatt
    16561 taaaatggct aggataaaag gataatccta tgtaatatta atgtaatctt tatgatttaa
    16621 tgattcgcat agtaatggag ttacatttta tatataatag taattgcgta agtaaataat
    16681 tggaggacta taaatgttaa ataatgaata taagaattcg tcattaaaga ttttttcatt
    16741 gaaaggaaac gaagcattag cgcaggaagt tgctgaccaa gtaggaattg aactaggtaa
    16801 atgttcagtt aaacgtttta gtgatggaga aattcaaatt aatatcgaag agagtattcg
    16861 tggttgtgac gtatttatta ttcaaccaac atcatatcct gtgaatctac atttaatgga
    16921 attattaatt atgattgatg cttgtaaacg tgcttctgca gcaacaatca atattgtagt
    16981 gccatattat ggatatgcaa gacaagatag aaaagcccgt agccgtgagc caatcactgc
    17041 taaattagtt gcaaacttaa tcgaaacagc tggcgcaact cgtatgattg cgttagactt
    17101 acatgcacca caaattcaag gattctttga tattccaatt gaccacttaa tgggtgtgcc
    17161 aattcttgct aaacatttca aagatgatcc gaatattaac ccagaagaat gtgtcgttgt
    17221 ttcaccagac catggcggtg ttacacgtgc acgtaaatta gctgacattt taaaaactcc
    17281 aattgcaatt atagataaac gtcgtcctag accaaatgtt gctgaagtga tgaacattgt
    17341 tggtgagatt gaaggacgta cggcaattat tattgacgat attattgata cagcaggtac
    17401 aatcacttta gctgcacaag cattaaaaga taaaggtgct aaagaggtat atgcttgttg
    17461 tacacaccct gttttatcag gaccggctaa agaacgtatc gaaaattctg ctataaaaga
    17521 attaatcgta acaaactcaa ttcatttaga tgaagatcgc aaaccatcta acactaaaga
    17581 attatctgtt gctggtttaa tcgcacaagc tatcattcgt gtatacgaaa gagaatcagt
    17641 tagcgtatta tttgactaat ctttaaaagg cgtttgacga acatattcca aacgtgtata
    17701 atagtttcgt tcgtgattat acgaataaat aaacacttgc aagcaacgat tatgttgatg
    17761 ggtaagtgag gtgctcgttt tgagcaaaaa tgaaaggtgg aaatgagaat ggcttcatta
    17821 aagtcaatca tccgtcaagg taaacaaaca cgttcagatc ttaaacaatt aagaaaatct
    17881 ggtaaagtac cagcagtagt atacggttac ggtactaaaa acgtgtcagt taaagttgat
    17941 gaagtagaat tcatcaaagt tatccgtgaa gtaggtcgta acggtgttat cgaattaggc
    18001 gttggttcta aaactatcaa agttatggtt gcagactacc aattcgatcc acttaaaaac
    18061 caaattactc acattgactt cttagcaatc aatatgagtg aagaacgtac tgttgaagta
    18121 ccagttcaat tagttggtga agcagtaggc gctaaagaag gcggcgtagt tgaacaacca
    18181 ttattcaact tagaagtaac tgctactcca gacaatattc cagaagcaat cgaagtagac
    18241 attactgaat taaacattaa cgacagctta actgttgctg atgttaaagt aactggcgac
    18301 ttcaaaatcg aaaacgattc agctgaatca gtagtaacag tagttgctcc aactgaagaa
    18361 ccaactgaag aagaaatcga agctatggaa ggcgaacaac aaactgaaga accagaagtt
    18421 gttggcgaaa gcaaagaaga cgaagaaaaa actgaagagt aattttaatc tgttacatta
    18481 aagtttttat actttgttta acaagcactg tgcttatttt aatataagca tggtgctttt
    18541 tgtgttatta taaagcttaa ttaaacttta ttgctttgta ctaaagttaa attaatttta
    18601 gtgagtaaaa gacattaaac tcaacaatga gacatcataa aaattttaat gtacttgatt
    18661 ttaaaatata tacttactaa gctaaagaat aatgataatt gatggcaata gcggaaaata
    18721 gatgttgtca ttataataat aaatgaaaca attatggtgg aggtaaacac gcatgaaatg
    18781 tattgtaggt ctaggtaata taggtaaacg ttttgaactt acaagacata atatcggctt
    18841 tgaagtcgtt gattatattt tagagaaaaa taatttttca ttagataaac aaaagtttaa
    18901 aggtgcatat acaattgaac gaatgaacgg agataaagtg ttatttatcg aaccaatgac
    18961 aatgatgaat ttgtcaggtg aagcagttgc accgattatg gattattata atgttaatcc
    19021 agaagattta attgtcttat atgatgattt ggatttagaa caaggacaag ttcgcttaag
    19081 acaaaaagga agtgcgggcg gtcacaatgg tatgaaatca attattaaaa tgcttggtac
    19141 agaccaattt aaacgtattc gtattggtgt gggaagacca acgaatggta tgacggtacc
    19201 tgattatgtt ttacaacgct tttcaaatga tgaaatggta acgatggaaa aagttatcga
    19261 acacgcagca cgcgcaattg aaaagtttgt tgaaacatca cgatttgacc atgttatgaa
    19321 tgaatttaat ggtgaagtga aataatgaca atattgacaa cgcttataaa agaagataat
    19381 cattttcaag accttaatca ggtatttgga caagcaaaca cactagtaac tggtctttcc
    19441 ccgtcagcta aagtgacgat gattgctgaa aaatatgcac aaagtaatca acagttatta
    19501 ttaattacca ataatttata ccaagcagat aaattagaaa cagatttact tcaatttata
    19561 gatgctgaag acttgtataa gtatcctgtg caagatatta tgaccgaaga gttttcaaca
    19621 caaagccctc aactgatgag tgaacgtatt agaactttaa ctgcgttagc tcaaggtaag
    19681 aaagggttat ttatcgttcc tttaaatggt ttgaaaaagt ggttaactcc tgttgaaatg
    19741 tggcaaaatc accaaatgac attgcgtgtt ggtgaggata tcgatgtgga ccaatttctt
    19801 aacaaattag ttaatatggg gtacaagcgg gaatccgtgg tatcgcatat tggtgaattc
    19861 tcattgcgag gaggtattat cgacatcttt ccgctaattg gggaaccgat cagaattgag
    19921 ctatttgata ccgaaataga ttctattcgg gattttgatg ttgaaacgca gcgttccaaa
    19981 gataatattg aagaagtcga tatcacaact gcaagtgatt atatcattac tgaagaagtg
    20041 attagccatc ttaaagaaga gttaaaaact gcatatgaaa atacaagacc caaaatagat
    20101 aaatcagtgc gtaatgatct gaaagaaacg tatgaaagct ttaaattatt cgaaagtaca
    20161 tactttgatc atcaaatact acgtcgttta gtagcattta tgtatgaaac accttcgaca
    20221 attattgatt atttccaaaa agatgcaatc attgcggttg atgaatttaa tcgtattaaa
    20281 gaaactgaag aaagtttaac agtagagtct gattcgttta ttagcaatat tattgaaagt
    20341 ggtaatggat ttataggaca aagttttata aaatatgatg attttgaaac attgattgaa
    20401 ggctatcctg tcacttattt ttcattattc gctacaacaa tgccgataaa actaaatcat
    20461 attattaaat tttcatgtaa acctgtccaa caattttatg ggcaatatga cattatgcgt
    20521 tctgaatttc aacgatatgt taatcaaaac tatcatatcg tggttttggt cgaaaccgaa
    20581 actaaagttg aacgtatgca agcgatgtta agtgaaatgc atattccatc aataacaaaa
    20641 ttgcatcgct caatgtcatc ggggcaagca gtgattattg aaggcagttt atctgaagga
    20701 tttgaactac ctgatatggg attagttgtc attactgagc gtgagctttt taaatcaaaa
    20761 cagaaaaagc aacgaaaacg tacgaaagct atctcaaatg ctgaaaaaat taagtcttac
    20821 caagatttaa atgtgggaga ttatattgtt catgtgcatc atggtgttgg tagatattta
    20881 ggtgttgaga cgctcgaagt agggcaaaca catcgtgatt atattaaatt gcaatataaa
    20941 ggtacggatc aactatttgt tccagtagat caaatggatc aagttcaaaa atatgtagct
    21001 tcggaagata agacgccaaa attaaataaa ctcggtggca gtgaatggaa aaaaacaaaa
    21061 gctaaagttc aacaaagtgt tgaagatatt gctgaagagt tgattgattt atataaagaa
    21121 agagaaatgg cagaaggtta tcaatatggg gaagacacag ctgagcaaac aacatttgaa
    21181 ttagattttc catatgaact tacgcctgac caagcgaaat ctatcgatga aattaaagat
    21241 gatatgcaaa aatcgcgtcc aatggatcgc ttgctatgtg gtgatgttgg ttatggtaaa
    21301 actgaagttg cagtgagagc agcattcaaa gctgtaatgg aaggaaagca ggttgcattt
    21361 ttagttccta caactatttt agctcagcaa cattatgaga cgttaattga gcgtatgcaa
    21421 gattttcctg ttgaaattca attaatgagt cgttttagaa cgcctaaaga gataaaacaa
    21481 actaaggaag gacttaaaac tgggtttgtt gacatagttg ttggtacaca caaattactt
    21541 agtaaagata tacagtataa agatttaggg ctgttgattg tagatgaaga acaacgattt
    21601 ggtgtacgcc ataaagagcg tattaaaaca ttaaaacata atgtagatgt actaactttg
    21661 actgcaaccc caatacctag aacattgcat atgagtatgc taggtgtgcg tgatttgtca
    21721 gtgattgaaa cgcccccaga aaatcgtttt ccagttcaaa catatgtatt agaacagaac
    21781 atgagtttta tcaaagaagc tttagaaaga gaactatccc gtgatggcca agtattttat
    21841 ctttataata aagtgcaatc catttatgaa aaacgagaac aactccagat gttaatgcca
    21901 gatgctaata ttgcagttgc tcatggacaa atgacagagc gcgatttaga agaaacgatg
    21961 ttaagtttta tcaataatga atatgatatt ttagtaacga cgacgattat tgaaacaggt
    22021 gtcgatgtcc caaatgcaaa tactttgatc attgaagatg cagatcgctt tggattgagt
    22081 cagttgtatc aattaagagg tcgtgttggt cgttcaagtc gtattggtta tgcatacttc
    22141 ttacatccag caaataaggt actaactgag actgcagaag atcgattaca agcgattaaa
    22201 gaatttacgg agttaggctc aggatttaag attgcgatgc gtgatttgaa cattcgtggt
    22261 gctggtaatt tgttaggtaa acaacagcac ggctttattg atacagttgg atttgatttg
    22321 tacagtcaaa tgttagaaga agctgtaaat gaaaagcgtg gtattgagga accagaatct
    22381 gaggtgccag aagtcgaagt tgatttaaac ttggatgcat atttgccaac agaatatatt
    22441 gcaaatgaac aagctaaaat tgaaatctat aaaaagctac gaaaaactga aacatttgat
    22501 caaattatcg acattaaaga tgaattaatt gatcgtttca atgattatcc tgttgaagta
    22561 gcacgtttgc ttgatatagt ggaaataaaa gtacacgcat tacattcagg tatcacgttg
    22621 attaaagata aagggaaaat aattgatatt catttatctg taaaagccac tgaaaatatt
    22681 gatggcgaag tactgttcaa agcaacacaa cctttaggta gaacaatgaa ggttggtgtt
    22741 caaaataatg caatgacaat tactttaaca aaacaaaatc aatggcttga tagtttgaag
    22801 tttttagtta agtgcattga agaaagtatg agaatcagtg atgaagcata aagaagcatt
    22861 taatggcgtt gtcgtgttaa ctgctgcatt aattgtcatt aaaattctaa gtgctgtata
    22921 tcgaattcca tatcaaaata tattaggcga tacaggtttg tatgcatatc aacaagtgta
    22981 tccaattgta gcattaggaa tgatattatc gatgaatgcc attcctagtg caattacaca
    23041 aaatataggg aagtatcata gtgacgaggc atatgcaaaa gcagtcgctt atatacaatt
    23101 agttggtata ttattattta ttgctatttt tgtgtttgcg aacaatattg cacatatgat
    23161 gggtgatagc catttaacac ctatgattca agcagcaagt ttaagcttta tatttatagg
    23221 tatgcttggc gtgttaagag gttattatca atctgcaaat aatatgacag ttccggctat
    23281 ttcccaggtt atagaacaag ttatacgagt aggtattatc attgttacta ttgttatttt
    23341 tgtagataga ggttggacga tatatgaagc gggaacaatt gctattttag catcaacgat
    23401 aggtttttta ggttcttcaa tttatttagt agcgcaccga ccttttaagt ttaaaatggt
    23461 aaataacact gcaaagatcg tttggaaaca gttcgcactt tcggttttga ttttcgctat
    23521 cagtcaatta atcgtaattt tatggcaagt aattgatagt gttactatta ttaagtcact
    23581 tcaagcgata cgcgtgccat tcgatgttgc cataaccgaa aaaggagtct atgaccgtgg
    23641 tgcatcattt attcagatgg gattgattgt aactacaaca tttagttttg cgctcattcc
    23701 tctgttaagt gacgcaatca aaatgaataa taaggtactt atgaatcgtt atgcaaatgc
    23761 gtcattaaag attacgattt taataagtac agcagcggga ataggattaa ttaatttatt
    23821 gcctttaatg aacggtgtat tttttaagac gaatgattta accttaacgt taagtgttta
    23881 tatgattacg gtcatttgtg tatcattaat tatgatggat atggcattat tacaagctca
    23941 acatgctgtg agacctattt ttgttggtat gacagcagga ttggttatta aatttatact
    24001 taatatcatt ttgattcgtt taagtggcat tattggtgcg agcattagca ctgttgtatc
    24061 attaattata ttcggtacga ttatccatat tgctgtaact aaaaagtatc atttgcatgc
    24121 gatgcgacga tttttcatga atgtcgtttt cggcatggta ttcatgtcaa tcgttgtcca
    24181 actcgtttta tttatagtga ccacacacgg tcgaataact ggactcattg aattgttatg
    24241 tgcagccttt ttaggtattg cagcattgtt ttactatatt tttaaattta atgtattgac
    24301 gtataaagag ttaacctatt taccattcgg ttctaaattg tatcaaatga agaaaggaag
    24361 acgttgatgg cacataccat tacgattgtt ggcttaggaa actatggcat tgatgatttg
    24421 ccgttaggga tatataaatt tttaaagaca caagataaag tttatgcaag aacgttagat
    24481 catccagtta tagaatcatt gcaagatgaa ttaacatttc agagttttga ccatgtttat
    24541 gaaacacatg accaatttga agatgtctat aatgatattg tggcgcaatt ggttgaaact
    24601 gctaatgaaa aagatattgt ctatgcggtt ccgggtcatc ctagagttgc tgagacaact
    24661 acagtgaaat tactggcttt agcaaaggac aatactgata tagatgtgaa agttttaggt
    24721 gggaaaagct ttattgatga tgtttttgaa gcagttaatg tagatccaaa tgatggcttc
    24781 acactgttag atgcgacatc attacaagaa gtaacactta atgttagaac gcatacattg
    24841 attacgcaag tttatagtgc aatggttgct gctaatttga aaatcacttt aatggaacga
    24901 tatcctgatg attaccctgt tcaaattgtc actggtgcac gaagcgatgg tgcggataac
    24961 gttgtgacat gcccattata tgaattggat catgatgaaa atgcattcaa taatttgacg
    25021 agtgtattcg taccaaaaat cacaacaccg acatatttgt atcatgactt tgattttgca
    25081 gcggaagtga ttgatacttt agttgatgaa gataaaggtt gtccatggga taaagtgcaa
    25141 acgcatgaaa cgcttaaacg ttatttactt gaagaaacat ttgaattgtt cgaagctatt
    25201 gacaatgaag atgattggca tatgattgaa gaactaggag atattttatt acaagtgtta
    25261 ttgcatacta gtattggtaa aaaagaaggg tatatcgaca ttaaagaagt gattacaagt
    25321 cttaatgcta aaatgattcg tagacaccca cacatatttg gtgatgctaa tgctgaaact
    25381 atcgatgact taaaagaaat ttggtctaag gcgaaagatg ctgaaggcaa acagccaaga
    25441 gttaaatttg aaaaagtatt tgcagagcat tttttaaatt tatatgagaa gacgaaggat
    25501 aagtcatttg atgaggccgc gttaaagcag tggctagaaa aaggggagag taatacatga
    25561 gattagataa atatttaaaa gtatcacggt taataaagcg acgtacgcta gcaaaagagg
    25621 taagtgatca aggtagaatt acaataaatg gtaatgttgc taaagctgga tcggatgtta
    25681 aagttgaaga tgtgctgacg attcgctttg gtcaaaaatt agtaacagtt aaagtaactg
    25741 cattaaatga acatgcatct aaagataacg cgaagggcat gtatgaaatc attgaagagc
    25801 gtcgacttga agaagcgtaa attggaggtg acaagcaatg aaaaataaag tagaacatat
    25861 agaaaatcag tacacgtcgc aagagaacaa gaaaaaacaa cgtcaaaaaa tgaaaatgcg
    25921 tgttgttcgt aggcgtatta cagtatttgc gggcgtatta cttgcgataa ttgttgtttt
    25981 atcaatcttg cttgttgtcc aaaaacatcg caatgatatt gatgcacagg agcgaaaagc
    26041 gaaagaagca cagtttcaaa agcaacaaaa tgaagaaatt gcgttaaaag aaaagttgaa
    26101 taatctgaat gacaaagatt atattgaaaa aattgcgcgt gatgattatt acttaagcaa
    26161 caaaggtgaa gtgattttta ggttgccaga agacaaagat tcgtctagct caaaatcttc
    26221 gaaaaaataa atccaaattg attcaaaatt atccgagtat agacattgtg aaaaaatcca
    26281 aacaaggata taataaggga aaatcgaatc aaatcgggag gatttattta acatatgtca
    26341 atcgaagttg gaaataagct taaaggtaaa gtcactggta ttaaaaagtt tggtgcattc
    26401 gtagaattac ctgaaggaaa aagtggttta gttcacatta gtgaagtcgc agataattat
    26461 gttgaaaacg tagaagagca cctttctgtt ggtgatgaag tagacgcaaa agtattatct
    26521 attgctgatg atggaaaaat tagtctttca attaagaaag ctaaagaccg tccacgtaga
    26581 caacatacga gtaaaccaag tcatcaaaaa ccagtgcaaa aagccgaaga ttttgaaaag
    26641 aaattaagca atttcttaaa agatagtgaa gataaattaa cttcaatcaa acgtcaaaca
    26701 gaatctagac gcggtggcaa aggttctaga cgttaattaa aataaataaa gactgtttcg
    26761 ataaggaata tatttagaat gatgcgtatc gaataatcga ttgcagcgtt agacaatcta
    26821 agactgtttc ttaaataagg agcagtctct tttatttgta atgatataac taagacttat
    26881 accatttttg aaaattgtaa aagtgaggtg atgttatgca gttaaatagt aatggttggc
    26941 atgttgatga ccatattgtt gtcgctgttt ctacaggtat tgatagtatg tgtttattgt
    27001 atcaattact aaatgattat aaagatagtt atagaaaact aacatgttta catgtcaatc
    27061 atggcgttag gtcagcttca atcgaggaag ccaggttttt agaagcatac tgcgaacgtc
    27121 atcacatcga tttacatatc aaaaagttgg atttgtcgca tagtctcgac cgaaataaca
    27181 gcattcagaa tgaagctcga attaaacgtt acgaatggtt tgatgagatg atgaatgtat
    27241 tagaagcgga tgtattgcta acggcgcatc atttggacga tcaattagaa actattatgt
    27301 atcgcatttt taatgggaaa tcaacgcgta ataaactagg gtttgatgag ttgtcgaagc
    27361 gaaaaggtta tcagatttat cgaccacttt tagctgtctc taaaaaagaa ataaaacaat
    27421 tccaagagag atatcatata ccatattttg aa
//
LOCUS       DAHHRR010000026        26161 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.026, whole genome shotgun sequence.
ACCESSION   DAHHRR010000026 DAHHRR010000000
VERSION     DAHHRR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26161
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.026"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            11..526
                     /locus_tag="NBI42_000917"
     CDS             11..526
                     /locus_tag="NBI42_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HCD1777352.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINRENYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            complement(672..1643)
                     /locus_tag="NBI42_000918"
     CDS             complement(672..1643)
                     /locus_tag="NBI42_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HCD1777353.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKVNPETKEIKQPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNIQDIIEDLATTYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVAPDFKTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDRDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGREEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            1887..2843
                     /locus_tag="NBI42_000919"
     CDS             1887..2843
                     /locus_tag="NBI42_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCD1777354.1"
                     /translation="MNYQVLLYYKYTTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEED
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPDITRFRDLPDW
                     IKENKELFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGQY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENEAK
                     YLGACSYECAKHERNRYVQANNISDNEWQHRLTNFDDLHQHA"
     gene            2993..3631
                     /gene="pcp"
                     /locus_tag="NBI42_000920"
     CDS             2993..3631
                     /gene="pcp"
                     /locus_tag="NBI42_000920"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCD1777355.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLENIIGTHTIDKLKLPTSF
                     KKVDTIINKTLASNHYDVVLAIGQAGGRSAITPERIAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTYVCNHLLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQIGGKPDTPSMTLENIVTGLTAAIEAISDDDDLRIALGTTE"
     gene            3734..4198
                     /gene="bstA"
                     /locus_tag="NBI42_000921"
     CDS             3734..4198
                     /gene="bstA"
                     /locus_tag="NBI42_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HCD1777356.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIGTILEGLQTLPER
                     VRKLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            4436..5854
                     /locus_tag="NBI42_000922"
     CDS             4436..5854
                     /locus_tag="NBI42_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCD1777357.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLSTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(6272..7189)
                     /gene="rarD"
                     /locus_tag="NBI42_000923"
     CDS             complement(6272..7189)
                     /gene="rarD"
                     /locus_tag="NBI42_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCD1777358.1"
                     /translation="MNTEYKKGIFLALSAYILWGILPIYWQFVDEIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGIIYVIYLWQQHQMSFGFNMSSFWL
                     LFSGAITAVPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTLSND"
     gene            7411..7986
                     /locus_tag="NBI42_000924"
     CDS             7411..7986
                     /locus_tag="NBI42_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1777359.1"
                     /translation="MNLPKIGKPATRALNAQGIHTLETASQHTKSFLSGLHGVGPKAI
                     SILEQALSEHNLHFKQESNHVLPFSLTGNVSCSHAPKCQQMIDFIVATATLDIELLRS
                     LVTPQFIWSVPGHFDIHGPQILIQELSEHQDHIESINIQSIITHGHLGALHGTQILKN
                     GTQIHFADFFEFEKHKKDAKVSKVTSYIVID"
     gene            8155..9171
                     /locus_tag="NBI42_000925"
     CDS             8155..9171
                     /locus_tag="NBI42_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HCD1777360.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGFVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFISSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSK"
     gene            9414..10220
                     /locus_tag="NBI42_000926"
     CDS             9414..10220
                     /locus_tag="NBI42_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCD1777361.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNSFFKAYLFQKGFNPQLMSATIHTPNGGRS
                     LNGSHASLVVPINNVFYVTDVGFGDLPLHAMPITSSDQTQPVTDISGTFRAIFENEHM
                     ELFYVQKFENDSWNTKYEAEFKPKQIEDFNSNIEYNQTNPDSIFVKHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFSEKYFGLNVTINRIENNNSKT"
     gene            complement(10342..10854)
                     /locus_tag="NBI42_000927"
     CDS             complement(10342..10854)
                     /locus_tag="NBI42_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HCD1777362.1"
                     /translation="MAKANQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGSQSWIVFMGVVDILFGLLVIFNVGASSAFFIYMFAFW
                     FIFSSIAGLFTFSGSGNLKLISVIFNVLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYIVDALA"
     gene            11136..11894
                     /gene="vraD"
                     /locus_tag="NBI42_000928"
     CDS             11136..11894
                     /gene="vraD"
                     /locus_tag="NBI42_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HCD1777363.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            11884..13764
                     /gene="vraE"
                     /locus_tag="NBI42_000929"
     CDS             11884..13764
                     /gene="vraE"
                     /locus_tag="NBI42_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HCD1777364.1"
                     /translation="MTFNHIIFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTILML
                     IIAYVLILFQSSLFLKRRSILSMMKDSIKTDVTTAKVTVKEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMISPFIILFLTVAGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVASQ
                     DAQQFESKLSQQHITFTKNYYETITVNNVKDQVITLENGSDSGHTNSILSANNKVTGN
                     NAIITNTKSLPNVINIHLNKDLVVKGTKNETFRVTQEDKDKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIIVIIVYTLIYIVFALIAFVHSNKLIKK
                     TI"
     gene            13858..14049
                     /gene="vraH"
                     /locus_tag="NBI42_000930"
     CDS             13858..14049
                     /gene="vraH"
                     /locus_tag="NBI42_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HCD1777365.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALAMIVIVLSTVTTPIIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            14441..14641
                     /locus_tag="NBI42_000931"
     CDS             14441..14641
                     /locus_tag="NBI42_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1777366.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(14768..15337)
                     /locus_tag="NBI42_000932"
     CDS             complement(14768..15337)
                     /locus_tag="NBI42_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1777367.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGITVAIIMGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVVGTLSLIIFLSVYLANKFL"
     gene            complement(15597..15992)
                     /locus_tag="NBI42_000933"
     CDS             complement(15597..15992)
                     /locus_tag="NBI42_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCD1777368.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLQMTHMWLLSIIVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKTGKSNSK"
     gene            complement(16060..16413)
                     /locus_tag="NBI42_000934"
     CDS             complement(16060..16413)
                     /locus_tag="NBI42_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCD1777369.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADNVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(16730..17569)
                     /gene="noc"
                     /locus_tag="NBI42_000935"
     CDS             complement(16730..17569)
                     /gene="noc"
                     /locus_tag="NBI42_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCD1777370.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(17612..18331)
                     /gene="rsmG"
                     /locus_tag="NBI42_000936"
     CDS             complement(17612..18331)
                     /gene="rsmG"
                     /locus_tag="NBI42_000936"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCD1777371.1"
                     /translation="MTVEWLAEQLKEHNIELTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(18331..20208)
                     /gene="mnmG"
                     /locus_tag="NBI42_000937"
     CDS             complement(18331..20208)
                     /gene="mnmG"
                     /locus_tag="NBI42_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCD1777372.1"
                     /translation="MAQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRKQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(20275..21654)
                     /gene="mnmE"
                     /locus_tag="NBI42_000938"
     CDS             complement(20275..21654)
                     /gene="mnmE"
                     /locus_tag="NBI42_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCD1777373.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(21797..22150)
                     /gene="rnpA"
                     /locus_tag="NBI42_000939"
     CDS             complement(21797..22150)
                     /gene="rnpA"
                     /locus_tag="NBI42_000939"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCD1777374.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(22270..22407)
                     /gene="rpmH"
                     /locus_tag="NBI42_000940"
     CDS             complement(22270..22407)
                     /gene="rpmH"
                     /locus_tag="NBI42_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCD1777375.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            23138..24499
                     /gene="dnaA"
                     /locus_tag="NBI42_000941"
     CDS             23138..24499
                     /gene="dnaA"
                     /locus_tag="NBI42_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCD1777376.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            24777..25910
                     /gene="dnaN"
                     /locus_tag="NBI42_000942"
     CDS             24777..25910
                     /gene="dnaN"
                     /locus_tag="NBI42_000942"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCD1777377.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLKLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
BASE COUNT         8697 a         4571 c         4004 g         8889 t
ORIGIN      
        1 gttttatatt atgactaact ttacttttga tggcgcacac agtagtttag aattccaaat
       61 taaacattta atggtttcta aagtgaaagg ttcatttgat caatttgatg tagcagttga
      121 aggcgatatt aatgatttca gtactttaaa agctactgca acaattattc caagttcaat
      181 taacactaaa aacgaagcac gtgataacca cttaaaatct ggtgatttct ttggtactga
      241 cgaatttgat aaaatcacat ttgtgacaaa atcagtatct gaaagcaaag ttgttggtga
      301 tttaacaatt aaaggcatca ctaacgaaga aacattcgac gttgaattca acggagtaag
      361 taagaatcct atggatggtt ctcaagtaac aggtattatt gttactggta caatcaatag
      421 agaaaactat ggcattaact ttaaccaagc acttgaaact ggtggagtaa tgctaggtaa
      481 agatgttaaa ttcgaagcat cagctgaatt ctcaatctca gaataacctc atctcataat
      541 cctcattgat tttaatatat atacaaaatg ccaactatat catccctagg tatagttggc
      601 attttttaac ttctaattaa aaatcgctaa gatttaaact tatattaaga cgcctttcat
      661 tcaggtaggt cttattgaaa ctgaaaactt tgatgacctt ttgcaaaacc attaactgta
      721 taaagcatag atccgccacc catttcaata tcatttgaac aaataatgag ttgatttgtt
      781 ccaggtataa attgtggatg tgtagaacgt aacatatgac cttcttcacg acctggtatc
      841 aatatttgtc ctattggata acccctttta ttaaatacta atactcggcc ttgaccatac
      901 attgccacat ataaattatc atctctatca atacaacatg agtctggtcc ttcatgacct
      961 gtaaaatagt acggtatggt agctccaaat ggttgtatcg tcacaccatc atcttcaagt
     1021 gcaatgcgat gtaatcgatt ggctgttgtt tctgttaccc atagtacttt ttcatctgta
     1081 cttaaagcaa taccatttgc tacgctaata ttttgaataa tcggcgtcac tgttttaaag
     1141 tccggcgcaa cataataaac gcctcctagt gggttggtag agtatcctct aaaatctgta
     1201 aaataaaatc cacctttaga atcaaatacc atgtcatcaa tacaatatgt tgttgcaaga
     1261 tcttcaataa tatcttgtat gttgtcacca ttttctgtag ctgcaaaaat gcctcctgta
     1321 gatttaaaat ctcctaaata acaaacgaat aatcggccat ctttatgtat tttgattgct
     1381 gcaggattcg ctttatgact tacaaaaggt tgtttaattt ccttcgtttc aggattgact
     1441 ttgaaaatat taccttcgaa tacatccaat aaaaatagtt gtccttgccg atcaaaattt
     1501 aatccttcta attgcaatcc tttttttgaa atttcaagcc atggttcagc tgtaattgtt
     1561 tgtaattcac tttcagatat aattggaaca gcactattag acttcccgct ataaaataat
     1621 gtaggtaaat cttgttgtga catcatgatc attcctttca tttcattcgt tttaactatc
     1681 atttttattt taaacaaggg aacatttaaa tcaaatgata acgttttcat ttatctattt
     1741 tatcggtcta gtggctgatt tcaagctaga aatattgaat gacaatacaa ctctgttaaa
     1801 atgatggacg tagacaaata tgcgtattga cgctttattt taaaaattaa catgcttata
     1861 acatgttttt agaaggagat taacctatga actatcaagt tcttttatat tataaatata
     1921 cgacgattga tgaccctgaa cagtttgctc aggatcactt agccttttgt aaagcacacc
     1981 atttaaaagg tagaattctt gtttctacag aaggtattaa cggcacatta tctggtacaa
     2041 aagaagaaac cgaacaatat atggcacata tgcatgccga tgaacgattc aaagatatgg
     2101 tgtttaaaat tgatgaagct gaaggacatg cttttaagaa aatgcatgta cgtcctcgaa
     2161 aagagattgt tgctttagat ttagaagaag acgtcgatcc aagacacaca actggtcagt
     2221 atttatcacc tgtagagttt agaaaagctc ttgaagatga tgacacagtc attattgatg
     2281 cacgtaatga ctatgaattt gatttaggtc attttcgtgg cgcaattcgc ccagacatca
     2341 cacgctttag agatttacct gactggatta aagagaacaa agagttattt gcagataaaa
     2401 aagttgtcac atattgtact ggcggtattc gatgtgaaaa attttctggc tggttattaa
     2461 aagaaggttt cgaagatgta gctcagcttc acggcggtat agctacatat ggtaaagatc
     2521 ctgaaacaaa aggtcaatat tgggatggta aaatgtatgt atttgatgac cgtatcagtg
     2581 ttgatatcaa ccaagttgaa aaaacaatta ttggtaagga ttggtttgat ggtaaaccat
     2641 gtgaacgcta tattaattgc gccaaccctg aatgtaataa gcaaatatta gtttcagaag
     2701 aaaatgaagc caaatattta ggtgcatgct cttatgaatg tgctaaacat gagcgtaatc
     2761 gttatgttca agcaaacaat attagtgata acgagtggca acatcgttta acaaactttg
     2821 atgatttaca tcaacatgca taagcaatta atacatttta aaacacgctt tgaaaatccg
     2881 atttataaag gtttttcaag gctgtttttt taagcgcata catagcgtgt tatgttttta
     2941 attatgcgca tgttatacta aattaaattt taatactgcg gggtgtctta aaatgcacat
     3001 tttagtaaca gggtttgcgc cttttgacaa tcaaaatatt aatccctcat gggaagctgt
     3061 gactcaacta gaaaatatta ttggcacaca tacaatcgat aaattaaaac taccaacctc
     3121 ttttaagaaa gtagatacta ttataaataa aacgttggca tctaatcatt atgatgttgt
     3181 actagctata ggacaagctg gtggtagaag tgccatcaca ccagaacgta ttgctataaa
     3241 tattgatgat gcgcgtattc cagataatga tgattttcaa cctattgatc aagccattca
     3301 tttagacggt gcgccagctt atttttcaaa tttaccagtt aaagcaatga ctcaaagtat
     3361 tattaaccaa ggacttccag gagcactttc aaatagtgca ggtacgtatg tatgtaatca
     3421 cttgctttat cacttaggct atttacaaga taagcactat cctcatcttc gctttggatt
     3481 tatccatgtg ccatacatac cagagcagat tggtggtaaa cctgatacac catcaatgac
     3541 attagaaaac attgttacag ggctgactgc agctattgaa gctatttctg atgacgatga
     3601 tttacgtata gctctaggca caacagaata gttataagag taacaacaaa atacattcct
     3661 taaatgacta acaaatttgt taatagggta atacttacgg gagtatgttt tatttatggg
     3721 ggaggaatta ataatgacta caaaaacggt atttgatgtc attgatatgg gattaggata
     3781 tttagtaaat gtgtatgatg cttggaaagt tgaaaaggtg cttgatgatt atcataagcc
     3841 tttttctaat accattcatt ggcaatttgg gcatgtatta acaatttttg aatcagcctt
     3901 agctgttgct ggtaaagaga atattgattt aaatatatat agacctttat tcggaaatgg
     3961 ttcgtctcca gatgaatgga aggacgaagt accgagtatt ggaacgattt tagaaggtct
     4021 tcaaacatta cctgaacgtg tacgaaaact aactgaagat gatttagcaa ttgaattgaa
     4081 acaaccaatt gtcggttgta ataacttaga agagttatta gtattaaatg ccattcatat
     4141 cccacttcat gctggtaaaa ttgaagaaat gtctcgtata ttaaaaaatt taaaataaat
     4201 atgtgcttat taaccgttaa caatacgtta acgggttttt tatttgttta aaaggccact
     4261 ctattaaatt taataaacac caatgatttc tcttcttcac tgcttctcga aaaataatta
     4321 tattaatgat ttcgttaatt gaattttata tttaattatt actgtacatc ttttgaagtt
     4381 agctttattc ttaaattgaa atatgtataa aacatagaaa gaaggacgtt ttaacatgga
     4441 aaacacggtt aaatatcgta agtttatact ccctatcgtt gtaggtctcc ttatttgggc
     4501 acttacacct tttaaaccgg atgctgtgga tccaacagca tggtatatgt tcgcaatatt
     4561 cgtcgcgaca atcattgctt gtattacaca accgatgcca atcggggctg tctctataat
     4621 tggatttaca atcatggtac tcgttggcat tgttgacatg aaaacggctg tcgctggttt
     4681 tggtaataat agcatttggt taattgctat ggcatttttc atttcgagag gatttgtgaa
     4741 aacaggtctt ggtagacgta tcgcacttca tttcgtcaaa ttatttggta aaaaaacatt
     4801 aggtttagcc tattctatcg tcggtgtaga tttaattcta gcgcctgcta caccaagtaa
     4861 tactgcgcgt gctggtggaa tcatgttccc aattatcaag tcactttctg aatcatttgg
     4921 ttcgaaaccg aaagacggat cagcacgcaa aatgggtgca tttcttgttt tcacagaatt
     4981 ccaaggtaat ttaattactg cggctatgtt tttaactgca atggccggta acccccttgc
     5041 acaaaattta gcatctagca catctaatgt tcacattaca tggatgaatt ggttcttagc
     5101 tgctttagtt cctggacttg tttccttaat tgttgtacct tttatcattt ataaaattta
     5161 tccaccaact gttaaagaaa cacctaatgc taaaagttgg gctgaaaatg aattagcgac
     5221 tatgggtaaa atcgctttag ctgaaaaatt tatgattggt atttttgtcg ttgcattaac
     5281 actatggatt gttggaagtt tcattcatat tgatgcaact ttaacggcct ttatcgcgct
     5341 agcattgtta ttatcgacag gcgtcttaac atggcaagac attttaaacg aaacaggtgc
     5401 ttggaacaca ttagtatggt tctcagtatt agtgttaatg gccgaccaat taaacaagct
     5461 tggatttatt ccttggttaa gtaaatccat tgctacaagt cttggtggct taagctggcc
     5521 tatagtactg gtcattttaa tattgttcta cttctattca cattacttat ttgcaagttc
     5581 tacagcacat atcagtgcga tgtatgcagc attactaggc gttgccatcg cagccggtgc
     5641 accaccatta ttcagtgcat taatgttagg tttcttcggt aacctattag cttcaacaac
     5701 acactatagt agtggtccag cgccgattct attctcttca ggttacgtga ctcaaaaacg
     5761 ttggtggaca atgaacttga tcttaggttt cgtctacttt attatctgga ttggtttagg
     5821 atcactttgg atgaaagtaa ttggtatatt ttaaaatatt taaattagcg ctcaaatctt
     5881 attgatttgg gtgcttttta atttgtattt aaaatctatc tttgctaaat caaagctccc
     5941 ctttttaaaa tacgttcata ctttaaatca tttgcgtgct tgactgaaaa tttgtataaa
     6001 gatttaagtc attgcataac atcacagaaa atacatttct atactattcc gcttcattga
     6061 ccagcattac gtatgccctc ataaatcatg aaataaaaaa gcaccttcgt ttaaatccat
     6121 tttaagtgcg aattcaacga aagtgattta tttcatattt aatgtttcaa atttatacag
     6181 ctgtcactgt tactgcacac atacctcagt cttaccttaa catggcacaa atagaaaatg
     6241 accacatata caatttagca actttctcac tttaatcatt tgatagtgtt tttgcgactg
     6301 gatgtttctt caatttaatg tattgagaaa gactatataa cacaatacct gtccaaataa
     6361 atataaacgt aattaattga tctatactaa aaggctcttt gaaaacaaat atgccgagta
     6421 caaacattat tgttggtcca acgtattgaa taaatcctat taacgaaagc ggaatacgtt
     6481 ttgccccggc tgagaataga attagtggta ctgccgtaat cgcacctgaa aataacaacc
     6541 aaaatgatga catgttaaat ccaaatgaca tttgatgctg ttgccataaa taaataacat
     6601 atattatgcc ggctggtgcg gtaacaatac attcaatcgt aatactgctg atggcatcaa
     6661 tatgtactac ttttttcaat aacccgtatg taccaaagga taacgctaat ataatagaaa
     6721 cgattgggaa ttctccaatc ttgagcgtca tatataatac accgatgaat gcgaataaaa
     6781 tggctagcca ttcaaattta ttgaatcttt cttttaaaaa gataagtgcg agcaaaatgc
     6841 taacaagtgg atttatataa taacctaaac ttgtttgtag gacgtgaccg ttcgttacag
     6901 cccaaataaa tgtaccccaa tttaatgtaa tgacatagcc tgctacgaca atcgctaata
     6961 gctgaatggg cttgcctaac aattgattca tatctcgttg aaatgcattg cgttgttttt
     7021 gtccaaccgc gagtatgaaa atcatgaata ttgctgaaaa tataatacga aaggctaaaa
     7081 tttcaaatgc gcctatttca tcaacgaact gccaatatat aggtagtatt ccccacagaa
     7141 tgtatgcact gagtgctaaa aatatacctt ttttatattc tgtattcact tacatacctc
     7201 cttactctcc caaattttta acatgttacg ctcacttagt taagctaata taacgatttt
     7261 actaataata tttcgataaa gatatcattt tgtttatatt tctcacattt attcaccaaa
     7321 cactaaacaa tattaatttt ataaataatt ctgtacaaat cagggtatat tgccagaaag
     7381 acaaccataa aacataaagg atgggtacat atgaatttac ctaaaattgg aaagcctgca
     7441 actcgcgcat taaatgcgca aggtattcat acattagaaa cagcctcaca acatacaaag
     7501 tcatttttat caggtttaca tggcgttggc cctaaagcta tatcaatttt ggaacaagct
     7561 ttaagtgagc ataatctgca ttttaaacaa gaaagtaacc acgtattacc attttcattg
     7621 actggtaatg tgtcatgtag tcatgcacct aagtgtcaac aaatgattga ttttattgtc
     7681 gctacagcca cactagatat agaattgtta cgatcactcg ttacacccca atttatttgg
     7741 tctgtccccg gtcattttga tatccatggc ccacaaatat taattcaaga actttccgaa
     7801 catcaagatc atatcgaatc tatcaatatt caatctatta tcacacatgg tcatttaggc
     7861 gccttacatg gtactcaaat tttaaaaaat ggaacacaaa ttcattttgc tgactttttc
     7921 gaatttgaaa aacataaaaa agacgccaaa gtgagtaaag taacatctta tattgtcatt
     7981 gattaataat gcaaactcta aataaaaatg ttaaaaaacc ttttattatt acatgtatat
     8041 ttttatataa caaagaattt tgtataacat ttttgtcata ttagtatttt ttattagagt
     8101 gattgttgta tttgcagtat gattaaatat catgttatga aagaaggata tattttgact
     8161 gtttttaaaa atgagcgctt aagctggtta ccatacatag ctattgtcat tttgttacac
     8221 gttattgggt ttagtttttt atggattgct ggaaaagacc atcatatctt atttggtatg
     8281 gggattcttg catatacatt aggtttgcgt catgcatttg atgcagatca cattgctgca
     8341 atagataata cggttcgcaa attattacag caacgcaaag atccatctgg tgttggtttc
     8401 tacttttcaa ttggacattc atctgtcgta tttttaatgg ctgttttttt aggggtatct
     8461 gtaaaatggg ctaaagatga attaccacat ttccaagata ttggtggaac gattggtaca
     8521 ctagtttcag gtttcttttt agtgcttatc ggtgtgttga atctaattat tttaatctct
     8581 ttaattaact tgttcgctaa attacgtcgt gaacacattg aagaagctga agtcgatgca
     8641 ttacttgaat ctagaggatt tgtttctcga tttgtaggac cttatttcaa attaatcaca
     8701 cgtagttggc acgtattgcc acttggcttt ttatttggac ttggttttga tacagctagt
     8761 gaaattgcgt tactcgctct ttcttcaggc gcatcacaac aagccatttc atttatcgga
     8821 atcttatctt taccaatttt attcgcatca ggtatgagtt tattggatac tttagatggt
     8881 gttgtaatga aatatgccta taattgggca ttcttcaatc ctattcgcaa aatctattac
     8941 aatataacga ttactgcgat atcagtcatg gcagcattag tgattgggat gattgaattg
     9001 ctacaaattc ttgctgataa actagactta catggtgcat tttgggcatt tattagttcg
     9061 atcgaatttg attatttagg ttatattcta gttgctttat ttttaattac ttggcttatt
     9121 tcaagtttaa tttggaagtt tggtcgcata gaacacaaat ggtctaaata aagtaacttg
     9181 tcttgcaact acctttgtat tgttacattt ttagacatcc tgcacacttc ttccattatg
     9241 ctacgctctc agtagtcatc tttaaatcgt ttcttttaga gatgacttca ttttgagagc
     9301 gtatttttct atttaaaatg tatttatcca aaattcaatg ctttcgaatt taaaatttca
     9361 gcatttctgt ttaaaattta attataatct ttaatacagg aggcgctatg cttatgaata
     9421 ttgcgaagtt agagaattat ttacaaattg attcatctcg ttataatcga ccgagtattg
     9481 aagcattaaa ttattatgca acacgtttta tgttaactgt accttttgaa aatattgatg
     9541 ttcaaaatag taagccgata tctataaata tcgatgcact ttttaataaa attgttcatg
     9601 ataaacgtgg tggtttttgt tatgaattga actcattttt taaagcatat ttattccaaa
     9661 aaggatttaa tccgcagtta atgtccgcta ccatccatac acctaatggt ggccgtagtc
     9721 taaatggttc acatgcgtca cttgttgtac cgattaacaa tgtattttat gtaacagatg
     9781 tcggttttgg agacttacct ctacacgcta tgccaattac ttcgtcagac caaactcagc
     9841 cagtcacaga cataagtggc acatttcgtg ctatttttga aaatgaacat atggaactat
     9901 tttacgtaca aaaatttgaa aatgatagct ggaatacaaa atatgaagct gaattcaaac
     9961 ctaaacaaat agaagatttt aatagcaaca tcgaatataa tcaaaccaac ccagattcaa
    10021 tttttgttaa acatttacta attacaatgc cacaatcatt tggacgcgcc acgatgtctg
    10081 aaaatcattt aactttaaca aggaatggta gctcagaaaa atttgatgtc actaaagata
    10141 actataaaca tttttcagaa aaatattttg gactaaatgt aactattaat cgtattgaaa
    10201 acaataatag taagacttaa aaatatgggc gtaacttact tattgtaggt agcgcccctt
    10261 tttttatgct tcataaatat aataaaaagg atatagcaca aacttcatgc cacacccttc
    10321 ttttgaaccg cttcatttta cttaagcaag tgcatctaca atataaataa cacctacgaa
    10381 tacaaatgca atagcaatca tcgtcgaaat aaagacgata cccattaatg gattaaatag
    10441 taaaatgaca ccgaaaacaa tacctaaaac gttaaagata actgagatta attttaagtt
    10501 accactacct gaaaatgtaa ataatcctgc aatagaacta aaaataaacc agaatgcaaa
    10561 catataaata aagaatgctg aacttgcgcc aacattaaaa ataactaata gaccaaatag
    10621 aatgtctaca acacccataa agacaatcca actttgactt ccacctacta aagctttggc
    10681 ttttctacga taaacaattt gaatcacacc attaatcaat acaaataatc caattaacca
    10741 tgtgattgca taaaagtttt caattggaaa tgtaaaaatg acgactgcca acattaataa
    10801 taatacaccc attatcaaac ttgaccattt tataccttga tttgcttttg ccataaaaat
    10861 caattccttt caaataaagc actcttttaa tttttacaaa aattttttat acaataatta
    10921 aattcccttt tcttaattta cacaaacatc gtcacttaga aagcaccatt gcttttgtct
    10981 aatatgtacg atgaatctgt atcctcactc aaaaatctac taagtagcga tagctttcaa
    11041 ctctgtaagg ttcattcatt gaattgtaag tttagataaa ggctactact ccaaacatta
    11101 tttaaaatga aattaaatac aaaaggagtg atactatgac gatattatca gtgcaacatg
    11161 tttcaaaaac atacggtaaa aagcacacat ttcaagcact taaagatatt aactttgaca
    11221 tacaaaaagg cgaattcgtt gcgattatgg ggccttctgg atcaggtaag acaaccttat
    11281 taaatgtact aagttcaatt gaccaaattt ctagcggtag cgtgattgct aacgggcaag
    11341 agcttaataa acttaatcaa aaagcacttg ccaaattccg taaagaatca ttaggtttca
    11401 tcttccaaga ttacagtatt ctgccgacat taaccgttaa agaaaacatt atgttacctt
    11461 tatctgttca aaaaatgtcg aaggcaacaa tggaagaaaa ttataaagcg atcacgacag
    11521 cattaggtat ttatgaccta ggaaataaat accctagcga attatctggt ggtcaacaac
    11581 aaagaactgc agcagcaaga gcatttgttc acaaaccaca aatcatattt gcagatgagc
    11641 caacaggcgc actcgactcg aaaagtgcaa atgacttatt acaacgtttg gaagaaatga
    11701 ataaatcgtt tgatacaact attgtcatgg ttacacatga tccggttgca gcaagttttg
    11761 cagaacgtgt catcatgcta aaagatggcc aaattcatac acaactttat caggaaggac
    11821 gttctaaaca ggccttttat gaagacattg tacatcttca atcagtatta ggtggtgtct
    11881 caaatgacat ttaaccatat cattttcaaa aacttacgcc aaaacttaaa acattatgca
    11941 atgtatttat tttcattatt ttttagcatc gtcttatatt tcagttttac aaccttacag
    12001 tttactaaag gtgtaaataa tgacgactcg atggccatca ttaagaaagg tgctttagtc
    12061 ggatcaatat ttttatttat cattattgtc atctttttaa tgtatgccaa tcatttattc
    12121 gtgaaacgcc gtacacgtga atttgcgcta tttcagttga ttggtttaac acgacaaaac
    12181 attttgaaaa tgttagcact tgaacaaatg attgtatttt taatcacagg tgttgtcggt
    12241 gtattatgtg gcattgcagg tgcacaatta ttgctttcca ttgtatctaa attgatgtca
    12301 ttatcgatta acttatcgat acatttcgaa cctatggccc ttgttttaac tattttaatg
    12361 ctaattattg cgtatgtact gattctattt caaagcagtt tgtttttaaa aagacgcagt
    12421 atcttatcaa tgatgaaaga ctcaattaaa actgatgtta caactgcgaa agttacagtt
    12481 aaagaagtca tttcaggcgt attaggtatt gctatgattg cactaggcta ttatatggcg
    12541 acagaaatgt ttggtacgtt caaagcacta actatggcta tgatatcacc gtttatcatt
    12601 ttatttttaa cagttgcagg tgcgtattta tttttcagaa gttctgtgtc acttattttt
    12661 aaaacattga aaaaatcaaa aaatggacgc gtatctatta cagatgttgt attcacatcg
    12721 tctattatgt accgaatgaa gaaaaatgcg atgtctttaa ctatcattgc aattatttct
    12781 gcagttacag taactgtatt gtgtttcgcg gcattatcta aatcaaatac agatcaaacc
    12841 cttacatcta tggcaccaaa tgaatttaac gtggtcgcct ctcaagatgc gcaacaattt
    12901 gaatctaaac taagccaaca acatattaca tttactaaaa attattatga aactatcact
    12961 gtaaataatg ttaaagatca ggttattact ttggaaaatg gtagtgattc aggtcacaca
    13021 aattcaatat taagtgcaaa taacaaggta acaggcaaca atgccatcat tacaaataca
    13081 aaatcgctgc ctaacgtgat taatattcat ttaaacaaag atttagtagt aaaaggtact
    13141 aagaatgaaa ctttccgtgt tacacaagaa gacaaagaca aggtttatcc cttaaatcta
    13201 agcttcaact cacctgtcat cgaagtaagt cctgaaaagt atcagcagtt gaaaacacaa
    13261 aataacgttc atacttttta tggatatgat attaaacaaa catcacaaaa ggaaaaagct
    13321 caagcaattg caaaacaatt tggagacaaa gtcatcactt atgatgaaat gaaaaaagag
    13381 gtcgatgcta ctaacggtat tctaatattt gttacatcat ttttaggatt agcattttta
    13441 gtagcagctg gatgtattat ttatattaaa caaatggatg aaactgagga tgaactaagt
    13501 aacttcagaa tattaaaacg cataggcttt acgcatacag atatgcttaa gggattatta
    13561 ttaaaaatta ctttcaactt cggtttacca ttattgattg ctatattaca tgcagtattc
    13621 gccgcaatag catttatgaa attgatgggt aatatatcct tcatgccggt tattatagta
    13681 atcattgtat acacactcat ttacattgtt tttgctttaa tcgcttttgt acattcaaac
    13741 aaattgatta agaaaaccat ttaacactta tgattaaaaa atgattcatt gagattttga
    13801 ttttaagcaa tgcagtataa ctattaactg attttataaa aaaggagaga ttgaatcatg
    13861 aaatttagag aattagtaaa acaatcatac gaggatttga aaaatttgac tgtaacttgg
    13921 tataacgtga ttgctttagc aatgattgtt attgtcttaa gcaccgttac aacaccaatt
    13981 ataggtattc cagctggttt attaggtggc gcctattatt taaaacgtcg tgaagaaaaa
    14041 ggtaaataaa cgcattccat cagtatatta cattgctttg accttttatg tcagttttta
    14101 atatcaacaa gtagcgttta tcgctgcttg ttttttatga attgcttata aaatcgtcat
    14161 gtttaaacga tatgttactt tgaaaacaaa caaataccat aaaagactac atttttttat
    14221 ttttataaac ccctttaaat caacgtttct aaggttttta aaaatctttt taaatttatt
    14281 ttcaaaaaac actgtacatt atgccaatat gagcgtatag ttggacttac gtaataaaag
    14341 ctcgtgaatt aaattgtagt gtatttgttt agaatatcct cttttttagt tatgaatttg
    14401 ttacaaatat taagtgcaaa agcacatgga ggttttctat atgaataacg gtacagtaaa
    14461 atggtttaac gcagaaaaag gttttggttt catcgaacaa gaaaatggcg gagacgtatt
    14521 cgtacatttc tcaggtatcg ctagcgatgg ctacaaaact ttagaagaag gtcaaaaagt
    14581 tactttcgaa atcactgaag gtcaacgtgg agaccaagca gttaacgtac aaactgttta
    14641 attttacaaa ataaaacgac tcattataaa tgaatcgctt tgattaccaa caaggttcta
    14701 aatcttgttg gtattttttt atgctatttt ggataatata aataatatga ggccatacca
    14761 cgatgattta taaaaactta tttgccagat acacactaag gaaaattatg aggctgagtg
    14821 taccaactac tgatataata gaaagcgcat ttgtcatcgt atttctagct ttagtcgttt
    14881 gtacttcact aggatctttt ccattcataa aagcaacaat tcgatcgtag ttgtccattt
    14941 tatgcacttt ttttaaatct tctattttca tagatgcata aaaacctatt cccataatga
    15001 ttgccaccgt tataccccaa gtccaattcc aataaacaac taaaggtccc attaaaattg
    15061 cagctaatgt catgaaaata agcatcacat atgtccataa gttaagactt cgttgcgctt
    15121 taatatcagg tacaaatgga atagtccctt ttactaaatc atctaaagtt acattgaaca
    15181 attcgcacat cattaataag ttatgtatgt ctggtaagct tttgtcattt tcccaattag
    15241 aaatactctg cctagataca tataactttt cagcaagata ttcttgtgaa taaccatctc
    15301 gttccctata ttttttaatt tgtttactca gattcaatta catacgcctc cattcaatta
    15361 gtacaatcat tacaatcaga ctatcagaca aagttaaatc aagtcacctg tatgattgac
    15421 taaatgtttt gacatacgac aacatcaggg ttcgaagact cccttaagac agacctgtat
    15481 gattacgcgc ttgtaatttt ctcaatgcca acttgaattt catttattaa tgatgcaact
    15541 tttccatgct attcaatgct tgcgaagtta gtcctattta agagattacg tttacactat
    15601 ttactattcg attttccagt tttccaacta tgtttttcta atcttttttg tgctataaat
    15661 tctaccgctt cgaaaataca tacagcactg ataaaccaac ttaaaaagcc aactataata
    15721 ctaagcaacc acatatgagt catctgtaac atcatccatg taactaaaat acatactaat
    15781 acccctgtca ttccaaacac tgcgccacga cttacatgca cagcgacttt atcaccatat
    15841 tgcatacctg taataaacat agacactaaa aatactgccg gaaacgttgc aaatataccg
    15901 ccaaattctt tccaaggtag tgttacagat acaatataac ttaataatac tgcaaatccc
    15961 cccacaaaaa atttcattaa tgttaatttc atgatgttct gcctccaata atttattact
    16021 taataactga atacaactaa ttatcacgca ttttccaaac tataaatgtc taatactgaa
    16081 tattgcgagg gaaataacaa accaacaaac aactgtgaat attgcgcctt tccgataacc
    16141 tttatgctta atatagagag atgtcaaaaa tacagttaat atgcaagaca gaataccgac
    16201 aattgctcca gtactaagat tcatcgacat ctccactaat tgcgtaccac gatgatctaa
    16261 tgctaatgcg ataatagccg caagaaagac tgccggcata gtagctataa tacctcctaa
    16321 cttaccacct acattatcag caataattga agccaatgca acagcgatac caccaatgac
    16381 aaaatgaaga attgcacttc caattgaaaa catcttcaac gtacctttct ataattaatt
    16441 caatacaaaa cgtatcattc acacaaagtg taaaacttaa attttctact gtaaaggcaa
    16501 ctcaaacgat gttcataaag ctttcatgat actttcataa actagcaata aagggttcaa
    16561 atgaaggtat atccagctca aattattgga aattaccatt aactgtaatt ttcaaatcaa
    16621 catataaata taaattttta tgtttcacgt aaaacaaaaa aagacaaagc tgttatgatc
    16681 ttagctttgt cttttatgtg ttagtcatca tgtatacgac atcctactac taacgtttat
    16741 atattcgaat ttttatttca taataatctt catgatcttt gtctttatgc tcaacatgta
    16801 atcctgtttg ttgaatcgct tggatacttt tgcctacctc atctcgtgct tgagtgacat
    16861 cttgtgcaaa gcgaaggttt tgtgctttga ctttttcggg ccccgttttt tggcgtactc
    16921 tatcttcagt ctgtttcaca tttagttttt gtgcaatgac ttgctcaatc aacgcttctt
    16981 gttcgctatc agacaatgat aataccgctc tcgcatggcg ttcagtaatt ttaccttctc
    17041 ttaagcgaag tagtactttc ggcgccaact tcaataaacg caacttattt gcaataaagc
    17101 tttgactttt acctaaactt tttgccaatt cactttgcgt tgtatcacca atttccaata
    17161 atttcttata ggcttccgct tcttcaacaa cagacaaatt ttctctttga atattctcaa
    17221 ttaatgccac aacagccgtc tcttcatcat ccatatcacg aataataacg tctgcttgag
    17281 gtaaatttag tgattgtatt gctctaaatc ggcgttctcc agcaataatt tcaaacatat
    17341 cttcttcaat cggtcttaca acaataggct gtaataaacc atgttcatgt attgattcag
    17401 caagttcttt aattttattt ggttcaaaca cctgtcttgg ttgataacgg ttgggaacga
    17461 tacgttcaat ttgaatggat tcaacattac tattgcgatc ttcttcaata tgtccaatga
    17521 tgtcatcttt gtttttcaaa ccaaataatt ttgaaaaagg ttttttcatt atccattcgc
    17581 tccttttatg ttagttgtaa aggattatgc attatttttc aagtaaagga gtcttattag
    17641 gcgtccctgg ttttcttgga tactttttcg gcgtctgtct ttttttatca ataatgaaca
    17701 tctggcgctc tccagcatct tctggcaatt caaaggtatg tgtttctgta acattaccac
    17761 ctaacacact aattgcaaat tttgcttctt ctaattcttc ttcacctttt gaagatttta
    17821 atgcaacaaa ctgtccacct tttttaacta gcggtaaaca caattcactc aacacggata
    17881 atctagctac tgctcttgca gtaacaacat cataagactc cctgtagaca cccttaccaa
    17941 atgtttctgc tctatcgtgt ataaagctga catcctgtaa ttgtaattct gacgctaaat
    18001 ggtttaaaaa ttgaatacgc ttatttaatg aatcaacaat cgtcactttt aactgcggaa
    18061 acattatttt taacggaata cttggaaaac cagctccagc gcctacatca catatactta
    18121 taggctgatt aaaatcaaaa taaaaactag gtgcaatgga atcataaaaa tgtttcaaat
    18181 atacatcgtg ttcatctgta atacttgtca agttcatctt ttcattccat tcaacaagta
    18241 aacgataata tgtttgaaac tgttgttttt gagtctcagt taattcaata ttatgttctt
    18301 ttaattgttc tgctaaccat tctacagtca ttagtcactc accctttgga gtttaccttg
    18361 ttctaagtaa atcaataata tagatatgtc tgctggattt acccctgata ttctagaagc
    18421 ttgtgcaata tttaaaggtt ttacttctga taatttttct cgcgcttcag tcgccaaact
    18481 atcaatctta ctataatcta agtcttctgg aattttcttc tcttccatac gcttaacttt
    18541 ctcaacttgt tgtagtgatt tattgatata accttcatat tttgtttgta tttctacttg
    18601 ttcttcaaca tctgcattca attgatgttc ttcttctaaa atttctaaaa ttatatcgta
    18661 agtcatttca ggtctgcgta ataaatcgat agctaaaata ccatctttta agcgagaacc
    18721 accatgttgt tcaataatcg cttgcgtatg ttcgtttggt ttaatacgaa tatctgataa
    18781 acgcttaatt tccgcatcaa tttgcttacg tttttcatta aaacgtgcat atcgttcttc
    18841 agaaatcata ccaagttcat atcccatatc cgtcaatctc aaatcagcat tatcatgacg
    18901 tagtaacaaa cgatattctg cacgtgatgt tagtaaacga taaggttcat tagtaccttt
    18961 agttacaaga tcatcgatta atacaccaat atatgcatct gaacgactta atatcttttc
    19021 gcctgtgttt aacactttac ctgcagcgtt aatacctgcc atcaatcctt gtcctgctgc
    19081 ttcttcataa ccagatgtac cattaatttg acctgcagtg tataagtttt taatcatttt
    19141 cgtttcaagt gtaggccata actgcgttgg cacaatcgca tcatattcaa ttgcgtagcc
    19201 ggcacgcatc atatctgctt tttcaagacc tggtatcgtc tctaacattt gacgttgcac
    19261 atgttcagga agacttgtag acaatccttg cacatatact tcatttgtat tacgaccttc
    19321 aggctctaag aaaagttgat gtcgcggctt atcattaaat cgaacaaatt tatcttcaat
    19381 tgaagggcaa taacgtggcc cggttccttt aatcatccct gaatacattg cagatagatg
    19441 taaattatca tcgataactt tgtgtgtttc agcattagta tacgttaacc aacatggcaa
    19501 ttgatctaat atatattctg ttgtttcaaa gctgaatgca cgacctacat cgtcacctgg
    19561 ttgtatttca gtcttcgaat agtcaattgt ttttgaattt acacgcggtg gtgtacctgt
    19621 tttaaaacga acaatatcaa aaccaagttc tcttaaattg tctgataatg tgattgatgg
    19681 taattggtga tttggtccac ttgaatactt catattacct aaaatgattt caccacgtaa
    19741 aaatgttcct gttgtaataa ttactgcttt agataaatac tctgtaccaa tatttgtacg
    19801 tacaccttta acttcattat cttctataat aagttcgtct accatacctt gcattatatg
    19861 caaattctct tcatcttcaa tcacgcgttt catttcttgt tgataaagta ctttatctgc
    19921 ttgcgctctt agtgctctta cagcaggtcc tttaccagta tttaacattc tcatttgaat
    19981 gtgtgtttta tcgattgttt ttgccatttg tccacctaaa gcatcaattt cacgaacaac
    20041 gataccttta gctggtcctc ctacagatgg gttacatggc ataaatgcaa tattatctaa
    20101 atttattgtt agcattaatg ttttagcacc acgtcttgca gatgctaaac ctgcttctac
    20161 acctgcatgt cccgcaccta taacgattac atcatattct tgagccacaa tataaacctc
    20221 cttatttgat atcttactag ccttctttag acggtattcc gtctaattca attactattt
    20281 acctaagcag aattgactga ataactgatc gatgagttca tcacttgcag tttcaccaat
    20341 gatttctcct aatatttccc aagttctagt taaatcaatt tgtaccatat ccattggcac
    20401 accagattct gctgcatcaa tcgcatcttg tatcgtttgt cttgcttgtt ttaataatga
    20461 aatatgtctt gaattagaaa cataagtcat atcttgattt tgtacttctc caccaaagaa
    20521 caaatctcga atttgtattt ctaattcatc aataccttct tgttttaaca ttgaagtttg
    20581 aattaatggc gtatcgccta tcatatcttt aacttcatta atatctatat tttgctctaa
    20641 atccatttta ttaacaatta cgattacatc ttcattttta accacttcat ataatgtgta
    20701 atcttcttga gtcaatgctt cgttattgtt taatacaaat aaaattaagt ctgcttggct
    20761 aagagccttt ctagagcgtt caacaccaat cttctctact atatcttctg tctcacgtat
    20821 accagcagta tcaactaatc ttaatggcac gccacgaaca ttgacgtact cttccaagac
    20881 atctctagtc gtacctgcta cctcagttac aatcgcttta ttatcttgta ttaaattatt
    20941 taacatcgat gatttaccta cgtttggttt accaacaata actgtagata aaccttcacg
    21001 cataatttta ccctgcgcac cggtatctaa taaacgatta atttcctgtt tgatttcttt
    21061 agactgctct aaaagaaatt cagtagtcgc atcttcaaca tcatcgtatt caggataatc
    21121 aatattcact tccacttgag cgagtatctc taatatagat tgacgttgtt ttttgattaa
    21181 gtcacttaga cgaccttcaa tttgattcat cgcaacttta gaagctctat ctgttttcga
    21241 gcgaataaag tccataactg cttcagcttg agataaatca atacgaccat ttaaaaaggc
    21301 acgttttgta aattcacctg gctcagccat tctagcgcca tatgtcatag taagttccag
    21361 cactctatta atagttaaaa taccaccatg acaattaatc tctataatat cttcgcgtgt
    21421 aaatgttttt ggcgctctta acacagacac cataacttct tcaactactt ctttcgactc
    21481 tggatcaata atatgaccgt aattaatcgt atgtgatgga acatcattta aaagatgttt
    21541 tcctttatat aatttgtcag caatttcaac ggcttgcggt ccagacaatc gaacaattcc
    21601 aattgcccct tcacccattg gtgttgaaat actcgtaatt gtatctaaat ccatattgct
    21661 actcgcctcc ttcaacgatg tgaatacatt ttaaagtaag ttattatacc ccttaggtag
    21721 tcttacgttt gcctgaggta agacttcggg tagtgttggg tggttaatgt tttactccct
    21781 ttcccctacc ctatccttac ttaatctttt tattaaaaac tttggcaatt ttaagtacgt
    21841 gctcaagact attctgtatt tgtaaagtcg tcatatcttt agctggctgt cttgctatta
    21901 caataatatc tttggccaat atatgcgact tatgtacttt gaaattttca cgtattgctc
    21961 ttttaatctt gtttcttaac actgcattac ctagtttttt agaaacacta atacctaagc
    22021 gaaaatggtc tatttcttta ttattacaag tgtatacaac aaattgtctg ttggctacag
    22081 aatgaccttt tttatatatt ctctgaaaat ctgcattctt tttaattcgg taagcttttt
    22141 ccaataacat cactcgctta tttatcgttt ttatttgaag ctatatttaa acttctattg
    22201 agcttataac ataaatttct atttattctt aatttaaacg aaaaaaagat cactgatgag
    22261 tcagtgatct tatgcagata aaactttacg gcctttacga cgacggcgcg ctaaaacttt
    22321 acggccattt tttgtgctca tgcgttttct gaaaccatga actttactat gtttacgttt
    22381 atttggttga taagtacgtt ttaccatgca aaacacctcc atctttatac agttatctaa
    22441 tataccagat tatgcagact ctgatcactt taatagataa gattttgcgt ttcttagatt
    22501 aaaattctcg tattagctca ttgattatct agtcataatt caagcaacta ctacaatata
    22561 acaaaatcct ttttataacg caagttcatt ttatactact gctcaatttt tttactttta
    22621 tcgattaaag atagaaatac acgatacgag cgattaaatt tcataacatc accatgagtt
    22681 ttgttcgaag catgagtgtt tacaatgttt gaatacctta tacagttctt atacatactt
    22741 tataaattat ttcccaagct gttttgatac acacactaat agatactcta tagaaggaaa
    22801 agttatccac ttatgcacat ttatagtttt cagaattgtg gataattaga aattacacac
    22861 aaagttatac tatttttagc aacatattca caggtatttg acatatagag aactgaaaaa
    22921 gtataattgt gtggataagt cgtccaactc atgattttat aaggatttat ttattgatat
    22981 ttacataaaa atactgtgca taactaataa gcaggatgaa gttatccacc gattgttatt
    23041 aacttgtgga taattattaa catggtgtgt ttagaagtta tccacggctg ttatttttgt
    23101 gtataactta aaaatttaag aaagatggag taaatttatg tcggaaaaag aaatttggga
    23161 aaaagtgctt gaaattgctc aagaaaaatt atcagctgta agttactcaa ctttcctaaa
    23221 agatactgag ctttacacga tcaaagatgg tgaagctatc gtattatcga gtattccttt
    23281 taatgcaaat tggttaaatc aacaatatgc tgaaattatc caagcaatct tatttgatgt
    23341 tgtaggctat gaagtaaaac ctcactttat tactactgaa gaattagcaa attatagtaa
    23401 taatgaaact gctactccaa aagaaacaac aaaaccttct actgaaacaa ctgaggataa
    23461 tcatgtgctt ggtagagagc aattcaatgc ccataacaca tttgacactt ttgtaatcgg
    23521 acctggtaac cgctttccac atgcagcgag tttagctgtg gccgaagcac cagccaaagc
    23581 gtacaatcca ttatttatct atggaggtgt tggtttagga aaaacccatt taatgcatgc
    23641 cattggtcat catgttttag ataataatcc agatgccaaa gtgatttaca catcaagtga
    23701 aaaattcaca aatgaattta ttaaatcaat tcgtgataac gaaggtgaag ctttcagaga
    23761 aagatatcgt aatatcgacg tcttattaat cgatgatatt cagttcatac aaaataaagt
    23821 acaaacacaa gaagaatttt tctatacttt taatgaattg catcagaata acaagcaaat
    23881 agttatttcg agtgatcgac caccaaagga aattgcacaa ttagaagatc gattacgttc
    23941 gcgctttgaa tgggggctaa ttgttgatat tacgccacca gattatgaaa ctcgaatggc
    24001 aattttgcag aagaaaattg aagaagaaaa attagatatt ccaccagaag ctttaaatta
    24061 tatagcaaat caaattcaat ctaatattcg tgaattagaa ggtgcattaa cgcgtttact
    24121 tgcatattca caattattag gaaaaccaat tacaactgaa ttaactgctg aagctttaaa
    24181 agatatcatt caagcaccaa aatctaaaaa gattaccatc caagatattc aaaaaattgt
    24241 aggccagtac tataatgtta gaattgaaga tttcagtgca aaaaaacgta caaagtcaat
    24301 tgcatatccg cgtcaaatag ctatgtactt gtctagagag cttacagatt tctcattacc
    24361 taaaattggt gaagaatttg gtgggcgtga tcatacgacc gtcattcatg ctcatgaaaa
    24421 aatatctaaa gatttaaaag aagatcctat ttttaaacaa gaagtagaga atcttgaaaa
    24481 agaaataaga aatgtataag taggaaactt tgggaaatgt aatctgttat ataacagcac
    24541 taataataac aatcattttt tacatttcta tatgctaatg tggcaagatg agcaaaactc
    24601 attttgtgga taatgtttaa aagtcataca caccatacac aagttatcaa catgtgtata
    24661 acttcgccaa atctatgttt ttaagactta tccaccaatc cacagcacct actactatta
    24721 ctaagaactt aaaacctata taattatata taaacgactg gaaggagttt taattaatga
    24781 tggaattcac tattaaaaga gattatttta ttacacaatt aaatgacaca ttaaaagcta
    24841 tttcaccaag aacaacatta cctatattaa ctggtatcaa aatcgatgcg aaagaacatg
    24901 aagttatatt aactggttca gactctgaaa tttcaataga aatcactatt cctaaaactg
    24961 tagatggcga agatattgtc aatatttcag aaacaggctc agtagtactt cctggacgat
    25021 tctttgttga tattataaaa aaattacctg gtaaagatgt taaattatct acaaatgaac
    25081 aattccagac attaattaca tcaggtcatt ctgaatttaa tttaagtggc ttagatccag
    25141 atcaatatcc tttattacca caagtttcta gagatgacgc aattcaattg tcggtaaaag
    25201 tacttaaaaa cgtgattgca caaacgaatt ttgcagtgtc cacctcagaa acacgcccag
    25261 tactaactgg tgtgaactgg cttatacaag aaaatgaatt aatatgcaca gcgactgact
    25321 cacaccgctt agctgtaaga aaattgaagt tagaagatgt ttctgaaaac aaaaatgtca
    25381 tcattccagg taaggcttta gctgaattaa ataaaattat gtctgacaat gaagaagaca
    25441 tagatatctt ctttgcttca aaccaagttt tatttaaagt tggaaatgtg aactttattt
    25501 ctcgattatt agaaggacat tatcctgata caacacgttt attccctgaa aactatgaaa
    25561 ttaaattaag tatagacaat ggggaatttt atcatgcgat tgatcgtgca tctttattag
    25621 cacgtgaagg tggtaataac gttattaaat taagtacagg tgatgacgtt gttgaattgt
    25681 cttctacatc accagaaatt ggtactgtaa aagaagaagt tgatgcaaac gatgttgaag
    25741 gtggtagcct gaaaatttca ttcaactcta aatatatgat ggatgcttta aaagcaatcg
    25801 ataatgatga ggttgaagtt gaattcttcg gtacaatgaa accatttatt ctaaaaccaa
    25861 aaggtgacga ctcggtaacg caattaattt taccaatcag aacttactaa aaataaatat
    25921 aaataaagga tgacgtgatt aattaaaacg tcatccttta ttttttggca aaaataattc
    25981 tagattcgta tgtaaaataa atttgacagc attttaaaca gcaaataaaa gacgccaatt
    26041 aaatttatga caaatgtatc caaaatttaa taagtgtact tatatgccct ttaaatttaa
    26101 aattttaata gtcaataaca agttgaatat aaaagttaaa cgtcgttaaa tagcgttaaa
    26161 a
//
LOCUS       DAHHRR010000027        25192 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.027, whole genome shotgun sequence.
ACCESSION   DAHHRR010000027 DAHHRR010000000
VERSION     DAHHRR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25192
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.027"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            136..1482
                     /locus_tag="NBI42_000943"
     CDS             136..1482
                     /locus_tag="NBI42_000943"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCD1777378.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            1504..2319
                     /gene="ccsA"
                     /locus_tag="NBI42_000944"
     CDS             1504..2319
                     /gene="ccsA"
                     /locus_tag="NBI42_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HCD1777379.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            2361..3287
                     /gene="hemC"
                     /locus_tag="NBI42_000945"
     CDS             2361..3287
                     /gene="hemC"
                     /locus_tag="NBI42_000945"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCD1777380.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTL
                     NGTNPVELGKKVSNKLKKQGAYEIIKRLNEQH"
     gene            3309..3977
                     /locus_tag="NBI42_000946"
     CDS             3309..3977
                     /locus_tag="NBI42_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCD1777381.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            3980..4954
                     /gene="hemB"
                     /locus_tag="NBI42_000947"
     CDS             3980..4954
                     /gene="hemB"
                     /locus_tag="NBI42_000947"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCD1777382.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            5002..6288
                     /gene="hemL"
                     /locus_tag="NBI42_000948"
     CDS             5002..6288
                     /gene="hemL"
                     /locus_tag="NBI42_000948"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCD1777383.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGHFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFTKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            6349..6451
                     /locus_tag="NBI42_000949"
                     /pseudo
     CDS             6349..6451
                     /locus_tag="NBI42_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6485..7558
                     /locus_tag="NBI42_000950"
     CDS             6485..7558
                     /locus_tag="NBI42_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCD1777384.1"
                     /translation="MTMIYRNNFIVFVLSFFISIILYSSHVLLPFMFGPIIASIICVK
                     VFRLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKDNWLSIIVVSISILVIALVMA
                     FIFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHVNGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYVIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKFRSNKRED"
     gene            complement(7781..8341)
                     /locus_tag="NBI42_000951"
     CDS             complement(7781..8341)
                     /locus_tag="NBI42_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCD1777385.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAHDIDRLMTFPNIVHHRKKIEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLKDCPSKPKHN"
     misc_binding    8399..8700
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8745..11375
                     /locus_tag="NBI42_000952"
     CDS             8745..11375
                     /locus_tag="NBI42_000952"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCD1777386.1"
                     /translation="MEMKPKYDPREVEAGRYEKWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVEDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNGNDEEQKQITRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            11388..12659
                     /locus_tag="NBI42_000953"
     CDS             11388..12659
                     /locus_tag="NBI42_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCD1777387.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPISEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPI
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            12921..13628
                     /locus_tag="NBI42_000954"
     CDS             12921..13628
                     /locus_tag="NBI42_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCD1777388.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSIIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            13625..14311
                     /gene="radC"
                     /locus_tag="NBI42_000955"
     CDS             13625..14311
                     /gene="radC"
                     /locus_tag="NBI42_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCD1777389.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIQVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKESCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDILTTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            14379..14663
                     /locus_tag="NBI42_000956"
     CDS             14379..14663
                     /locus_tag="NBI42_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777390.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTVGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(14780..14977)
                     /locus_tag="NBI42_000957"
     CDS             complement(14780..14977)
                     /locus_tag="NBI42_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777391.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            15137..15610
                     /locus_tag="NBI42_000958"
     CDS             15137..15610
                     /locus_tag="NBI42_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HCD1777392.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QTNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ"
     gene            15684..15803
                     /locus_tag="NBI42_000959"
     CDS             15684..15803
                     /locus_tag="NBI42_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777393.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNR"
     gene            16001..16843
                     /gene="mreC"
                     /locus_tag="NBI42_000960"
     CDS             16001..16843
                     /gene="mreC"
                     /locus_tag="NBI42_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCD1777394.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            16843..17373
                     /gene="mreD"
                     /locus_tag="NBI42_000961"
     CDS             16843..17373
                     /gene="mreD"
                     /locus_tag="NBI42_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCD1777395.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    17400..17722
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            17735..18043
                     /gene="rplU"
                     /locus_tag="NBI42_000962"
     CDS             17735..18043
                     /gene="rplU"
                     /locus_tag="NBI42_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCD1777396.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            18049..18369
                     /locus_tag="NBI42_000963"
     CDS             18049..18369
                     /locus_tag="NBI42_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCD1777397.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            18381..18665
                     /gene="rpmA"
                     /locus_tag="NBI42_000964"
     CDS             18381..18665
                     /gene="rpmA"
                     /locus_tag="NBI42_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCD1777398.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            19156..20448
                     /gene="obgE"
                     /locus_tag="NBI42_000965"
     CDS             19156..20448
                     /gene="obgE"
                     /locus_tag="NBI42_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCD1777399.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGKELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLILFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            20461..20916
                     /locus_tag="NBI42_000966"
     CDS             20461..20916
                     /locus_tag="NBI42_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCD1777400.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPLEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            20930..21532
                     /gene="ruvA"
                     /locus_tag="NBI42_000967"
     CDS             20930..21532
                     /gene="ruvA"
                     /locus_tag="NBI42_000967"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCD1777401.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            21564..22568
                     /gene="ruvB"
                     /locus_tag="NBI42_000968"
     CDS             21564..22568
                     /gene="ruvB"
                     /locus_tag="NBI42_000968"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCD1777402.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDDESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            22570..23595
                     /gene="queA"
                     /locus_tag="NBI42_000969"
     CDS             22570..23595
                     /gene="queA"
                     /locus_tag="NBI42_000969"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCD1777403.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            23618..24757
                     /gene="tgt"
                     /locus_tag="NBI42_000970"
     CDS             23618..24757
                     /gene="tgt"
                     /locus_tag="NBI42_000970"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCD1777404.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFVDDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            24776..25036
                     /gene="yajC"
                     /locus_tag="NBI42_000971"
     CDS             24776..25036
                     /gene="yajC"
                     /locus_tag="NBI42_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCD1777405.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
BASE COUNT         8816 a         3438 c         4856 g         8082 t
ORIGIN      
        1 ttttcaacgt tcatcatttt gtcaaaaact caaaagtaaa ttagaaagat tataatttat
       61 ttaagcatcg tacttaattg gattttaaat tatgttataa tatttgtatt gttagtatat
      121 atgggggcgt ttcaaatgca ttttattgca attagtataa atcatcgcac agctgatgtt
      181 gcactaagag agcaagttgc ttttagagat gatgccttac gaattgccca tgaagattta
      241 tatgaaacta aatctatttt agaaaatgtc atattatcaa catgtaatcg aactgaagta
      301 tatgctgttg ttgatcaaat tcacacaggt cgttactata ttcaacgatt tctagctcgt
      361 gcatttggat ttgaagtaga tgatattaaa gcaatgtcag aagtaaaagt gggggacgaa
      421 gcagtagaac atttattgcg tgtcacttct ggtttagatt caatcgtact tggagaaact
      481 caaattttag gtcaaataag agatgcattt ttcttagcgc aaagcacagg tacgacagga
      541 acaattttta atcatctatt taaacaggca attacttttg caaaaagagc acataatgaa
      601 acagatatag ctgataatgc tgtaagtgtg tcttatgctg cggtcgagtt ggcgaaaaaa
      661 gtatttggca aattgaaaag taagcaagct atcattattg gtgcagggga aatgagtgaa
      721 ttatcactat taaatcttct tggttctgga attactgata ttacagtagt aaatagaaca
      781 attgaaaatg ctatgaaatt agcagcaaag catcaagtga aatatgatga actatcatca
      841 ttaccaaatt tacttgaaag tgcagatatt gtgattagtt caacgagtgc acaatcttat
      901 atcattacaa atgaaatgat agaaagaatt gcagaaaata gaaagcaaga ttcactagta
      961 ttgattgata ttgcagttcc tcgagatatt gaacctggta ttagtgccat cacaaacatc
     1021 tttaattatg acgttgatga cttaaaaggt ttagttgatg caaacttacg tgagcgacaa
     1081 ttagcggctg caacaatttc ggaacaaatt cctgcagaaa tacatgcaca caatgagtgg
     1141 attagtatgt tgggtgttgt tccggtgatt agagctttac gtgaaaaagc tatggcaatt
     1201 caagcagaaa cgatggacag tattgatcgt aaattgccag ggttaagcga aagagaacgt
     1261 aaaattatat ctaaacatac aaaaagtatc atcaatcaaa tgttgaaaga tcctattaaa
     1321 caggccaaag aattaagtag tgataagaaa agtaatgaaa aattagagct atttcaaaat
     1381 atatttgata tcgaagctga atgtcctcat gagcaagcga agcaacaaaa agaaagcaag
     1441 gtcaaggaaa tttcagcacg acgtatcttt agttttgaat aagcatatta atatggtgat
     1501 aatatgcaag aaaacctgtt tattcgattc aatgaaatta tattattaat atacttaatc
     1561 agtatcattt gctattttta tgattttgtc caaaaaagtc ataagattag aagtttaggc
     1621 atatatttat tggggattgt ttgggtttta caaacaatct ctttatctat ttttattata
     1681 caaactagac atattccatt agggtctatt tcagatgtat tctatacttt aagttggctg
     1741 attatttcaa tctctttaat tcttaacctt atcaaagttt tgaatttctc tgtgtttttc
     1801 ttgaatttaa tagggttaac acttttaggg atgaatacat ttcaaccgac acattatact
     1861 aataaggttc agaaaattgc tgttgtagac gagttactgt tagtccatat tggtctagct
     1921 gtgttaagtt atgtcttctt tgccttagct tttgtcaatg cattactgta tatcatgcaa
     1981 taccgtaatt tgaaagaaaa gcgttttgat caaaaatact ttagaatagg tagcgttgct
     2041 acacttgaat caattgtgtt ttactcaacg ttaagtgggt ggataattct catatttagt
     2101 attattttag gtacacagtg gggtgtaatt tcagttggtg aacgtatatt cattgacccg
     2161 aaggtaatac tctcttcaat tattacagta ttatatggaa gttatatttt acttagaatc
     2221 aacaaatggt tgaattcaag gtatttaata tactataaca ttattttatt ttgcttaaat
     2281 atgattaatt tattttttgc aacacatttt gtgaattgag aaaataatct agagcaatcc
     2341 gttcctagga ggaattgaac atgcgtaaat tagtcgttgg ctccagaaga agtaaattag
     2401 ctttaacaca aagccagcaa tttattgata aattaaaagc tgtcgagcca aatctagaaa
     2461 ttgaaattaa agaaattgtc acgaaaggcg atcgtatagt agataaacaa ttgtctaaag
     2521 tcggaggtaa aggcttattt gttaaagaaa tacaacatga actttttgaa aaaaatatcg
     2581 atatggcaat acactcgctt aaagacgtac caagtgtaat tccggaaggt ttaacattag
     2641 gttgtatccc tgatagagaa ttaccttttg atgcgtatat ttctaaaaca catacaccac
     2701 tatcccaatt gccagaaggc agtattattg gtactagttc attacgtcgt ggtgcacaaa
     2761 tattatctaa gtatcctaat ttagagatta aatggattag aggtaatata gatacacgat
     2821 tagaaaagtt acaaactgaa gattatgatg cgattatttt agctgcagct ggtttaagaa
     2881 gaatgggctg gtcagatgat atcgtaacat cttatcttga tagagataca ttgttacctg
     2941 caatcggaca aggtgcttta gggatagaat gtcgtagtga cgatgaagaa ctattaacat
     3001 tattaagcaa agtacataat gatgaggttg caaaatgtgt gactgctgaa cgaacgtttt
     3061 tagcagaaat ggatggtagt tgtcaggtgc caattgcagg atatgctaca atctcagatc
     3121 aaaaagaaat cgaatttaca ggtttaatta tgaccccaga tggtaaagaa cgatttgaat
     3181 atacattgaa cggaacaaat ccggttgagt taggcaaaaa agtgagtaac aaattaaaaa
     3241 agcaaggtgc ttatgaaatt ataaaacgct taaatgaaca acattaaata atggagtgac
     3301 gtttaaatat gaagccagtt gtagttatga cacaaacaaa tgacatgcaa agtgatttag
     3361 tatcaattat tcacaaaccc tttattgata taaagccact aaattttgat attcatttgt
     3421 taaatcaacg ctatgactgg cttatttttt catctaaaaa tgctgtgaaa ttcttttata
     3481 aatatctaaa aggaattaat gttgataaca ttgctgtgat aggaagtaag acagcgcaat
     3541 attgtgaatc acttggcatt cgagttgatt ttatgccaaa cgacttttct caagaaggat
     3601 ttttaaaatc atttaatcaa actaaccaaa aaatactttt gccttcgagt gaattggcga
     3661 gaccattgtt attagcagcg ttatctaaag ataatgaagt tgttaaaata gatttatata
     3721 cttcagtgcc taacaaacaa aatatacaag atgtcaaaga aatgatagaa catcaacaaa
     3781 tcgatgcatt aacattttca agttcgtcgg cagtacgtta ttattttaat gaaggatttg
     3841 taccaaaatt caagtcgtat tttgctattg gagaacaaac agcacggacc attaaatcat
     3901 atcaacaacc agtaacaatt gcagaaattc aaacactcga atcactaatt gaaaagattt
     3961 tagaaagtag gggctaaaaa tgaaatttga tagacataga agattgagat catcagcgac
     4021 aatgagagat atggttagag agaatcatgt aagaaaagaa gatttaatct atccaatttt
     4081 tgtagttgaa aaagacgatg taaaaaaaga aattaagtca ttgccaggtg tataccaaat
     4141 cagtttgaat ttacttgaaa gtgaattaaa agaagcttat gacttaggca tacgtgccat
     4201 tatgtttttc ggtgttccaa actcaaaaga tgatataggt accggtgcat acattcacga
     4261 tggtgttatt caacaggcaa cacgtattgc taaaaaaatg tatgatgact tattaattgt
     4321 tgcagacact tgtttgtgtg aatatactga tcatggtcat tgtggtgtga ttgatgatca
     4381 tacacatgac gttgacaatg ataaatcatt gccactactt gttaaaacag caatttctca
     4441 agtggaagct ggtgctgata ttattgcgcc aagtaatatg atggatggtt ttgttgctga
     4501 aattcgtcgt ggattagatg aagccggcta ttacaatatt cctataatga gttatggtgt
     4561 caagtatgca tcaagtttct ttggaccttt tagagatgca gcagattcag cgccatcatt
     4621 tggtgacaga aaaacgtatc agatggaccc tgctaaccgt ttggaagcac ttcgtgaatt
     4681 agaaagtgat cttaaagaag ggtgcgacat gatgattgtt aaacctgctc taagttattt
     4741 agacatagtt cgagacgtta aaaatcatac gaatgttcca gttgttgcat ataatgtgag
     4801 tggagaatat agtatgacta aagcagcggc acaaaatggt tggatagatg aagaacgtgt
     4861 cgttatggaa caaatggttt caatgaaacg tgcaggtgct gatatgatta ttacgtattt
     4921 tgcaaaggac atttgtcgct atttagataa ataaggtttt atatgtatga ttttccataa
     4981 actataggag gaatttactt tatgagatat acgaagtcag aagaagcaat gaaggttgct
     5041 gaaactttaa tgcctggtgg tgtaaatagt ccagtacgcg catttaaatc agtagataca
     5101 ccagcaattt ttatggacca cggtaaaggc tcaaaaattt atgatatcga tggtaacgag
     5161 tatatcgact atgtactaag ttgggggccg cttattttag gacatagaga ccctcaagtt
     5221 attagtcatt tacatgaagc aattgataaa ggtacaagtt ttggtgcatc aacattactt
     5281 gaaaataaat tggcgcagct cgtaattgac cgagtacctt caatagaaaa agtgcgtatg
     5341 gtgtcatctg gtacagaagc tacattggat actttaagat tagcacgtgg ttatactggc
     5401 agaaataaaa ttgtgaaatt tgaaggttgc tatcatggtc atagtgattc gttattaatc
     5461 aaagctgggt ctggggtggc aacattagga ttgccggatt ctcctggtgt gcctgaaggt
     5521 attgctaaaa atacaattac agttccatac aatgatttag atgcacttaa aatcgctttc
     5581 gaaaaatttg gagacgatat tgctggtgta atcgtagaac ctgttgctgg taatatgggt
     5641 gtcgtaccgc cgatcgaagg ttttttacag ggattaagag atattacgac tgaatacggc
     5701 gcattgctaa ttttcgatga agtaatgact ggtttcagag tcggttatca ttgtgcacaa
     5761 ggtcactttg gtgtgacacc agatttaact tgcttaggaa aagttatcgg tggaggacta
     5821 cctgtaggtg cttttggtgg taaaaaagaa atcatggatc atatagcacc attaggaaat
     5881 atttatcaag ctggtacctt atcaggaaat cctcttgcaa tgacaagtgg ttatgaaacg
     5941 ttaagtcaat taacgccaga gacatatgag tattttaata tgttaggcga tatacttgaa
     6001 gatggtttaa agcgtgtatt tactaaacac aatgtaccaa taactgtaaa tagagcaggt
     6061 tcaatgattg gttatttctt aaatgaagga cctgtaacta attttgaaca agcgaataaa
     6121 agtgatttga aattatttgc agaaatgtat cgtgaaatgg caaaagaagg tgtattttta
     6181 ccaccatctc aatttgaagg tacattctta tctacggcac acacgaaaga agatattgaa
     6241 aaaacgattc aagcatttga tacggcttta agtcgtattg taaaataaat atacggacaa
     6301 attgagagcc tgaactttgt tcaggctctt tttaaatgta tatcaggtat gggcagcgac
     6361 ttgatagtga aagtccatat atgctttgta gtcaaaactg ctagcggata ttgttatctt
     6421 aaacaaacat gaagctcaag cagcaattta gtcattttat tttttattga aagaagtgga
     6481 aaacatgaca atgatatata gaaataattt cattgtgttc gttttatcat tttttattag
     6541 tattatattg tattcatcgc acgtattact cccatttatg tttggtccta ttatcgcatc
     6601 aatcatttgt gtaaaagttt tcagacttga tattaaatgg ccattcttac ttagtgaatt
     6661 agggattgta ctattaggtg tgcaaatcgg atcaacgttt acgaaaaatg ttgttatgga
     6721 tattaaagac aattggcttt cgattattgt tgtatctatt tcgatattag taattgcatt
     6781 agtaatggca tttattttca aaaaaattgc acgtattaat acagaaacag ctattttaag
     6841 tgttatacca ggagcactaa cacaaatgct ggtcatggct gaacaagaca aacgtgctaa
     6901 tttgttagta gttagcttaa cgcaaacatc acgaattata tttgttgttg ttttagtacc
     6961 gttcatttca tatttttttc atgatggtaa catgcatgtg aatggtaagt taacaaaagt
     7021 cttgccttta tcacaagtat taaacatagg gcaaatagtt attttagcga tagctatctt
     7081 tatagtttat ctaattatgt ctaaaataaa gtttccaaca tttcaattat tagcaccact
     7141 tattgtatta attgtttgga atttttctac aggtttaaca tttacactag atcattggtt
     7201 gttgaacatg gcacaactaa tatatatgat tagaattgga gttcaaattg cgcatttatt
     7261 gtcagattta aaaggtagac tagcaatcgc aattacaatt caaaatatta tgttgataat
     7321 tggtgcgcta atcatggttt atgtcataca tttctttgat aataatccta taaatgaatt
     7381 gtttcttggt gcagcaccag gaggggttaa tcagatagtt ttagttgcat tagctactgg
     7441 tgccgatgtt gctatgatta cgagttatca tattttccga atctttttca tcctatttat
     7501 tattgcacca ggaattaatt actttttaaa atttcgttcg aataagcgtg aagattagaa
     7561 actatcatcc atttaagtta aattatgctt aggatagaat tcattattaa ggtttaagca
     7621 aaggttgtac atctattttt agattaagat atttattatt agcagtgcat taacatggaa
     7681 ctttaacttg tgatttgaat aaatttctac attaaaaaac gaggcagtct attagttaaa
     7741 aatagatgat gtctcgttta ctttatgatg ctatagcaaa ttaattgtgt ttaggctttg
     7801 atggacaatc ttttaaatgt gcatcatata aaccggctgc ttctaaaaac gaaaatactg
     7861 ttactggacc taaaaattta aacccgtatt gttttaaatc tttagatagt tgtgttgcag
     7921 tatcatcaac tgtgatacga tcagaagcat gttcatactg caaatcttta ggcttaccat
     7981 ttacataaga ccataaaaat ttactaaaac taccataagc ttgttcaatt tttaaatacc
     8041 cttgagcttg attaacaatt gcttctattt ttttacgatg atgaacgata tttggaaaag
     8101 tcattaagcg gtcgatatca tgagcggtca tttgtgctac cttttctggt tcgaaatcat
     8161 aaaatgcttc ttcataggct tctttctttt ttaaaatagt taaccaagat agcccagcat
     8221 gttgtgattc taatgctaaa agtttaaaca atgccttgct atcatagagc ggttgtcccc
     8281 atacatgatc atgatagtct aagtagactg gatctttagt tccaaatgcg cattcattca
     8341 ttttattccc acctatacat tgacttattt tgtcaatcat tatactataa gttaagagtt
     8401 taatatagga tttgggaaga gtaagttcaa attattttaa agagagcata tgatttggtg
     8461 tgagtatgta aatgattttt actgaaggta gcccacagag tatcttagct gaacgtgatt
     8521 aatagtaggc taagacgtgt ttgagcgtta atcataatct agagtgcttg aagagatgaa
     8581 gcatttcaaa tttcacgatg atagtattgg tatgattatt ccaattattt taaaagagtg
     8641 aatttgatat taatcatcct tcttgaatat aggtggtacc acggaatatc cgtcctattt
     8701 gtatatagga tggatatttt ttattttttt aggaggtata gcaaatggaa atgaaaccaa
     8761 aatatgatcc tcgtgaagtt gaagcgggac gttatgaaaa atgggtaaag aatggttatt
     8821 ttaaaccgtc agaagataaa tcaaaagaaa catatacaat tgttatcccg ccaccaaatg
     8881 taactggtaa attacattta ggacatgcat gggatacaac tttacaagat atcattacac
     8941 gtatgaaacg tatgcaagga tacgatacgt tatacttacc aggtatggat catgctggta
     9001 ttgcgacaca ggcaaaggta gaagctaaat taaatgaaca aggaataact agatatgatc
     9061 ttggtcgtga aaagtttcta gaacaggcat gggattggaa agaagagtat gcgtcattta
     9121 ttcgtgcgca atgggctaaa ttaggtctag gtttagatta tagtagagaa cgttttactt
     9181 tagatgaagg tttaagtaaa gcagttaaaa aagtttttgt tgacttatac aataaaggaa
     9241 ttatttatcg tggcgaacgt attataaatt gggatcctaa agcacgtaca gctttatctg
     9301 atattgaagt aatacatgaa gatgttcaag gtgcgtttta tcattttaaa tatccttacg
     9361 ctgatggtga aggttttatt gaaattgcaa caacaagacc agaaacgatg ttaggtgata
     9421 cagcaattgt tgttaaccct aatgacgaac gatacaaaga tgtaatcggt aaaactgtta
     9481 tattaccaat cgtaggacgc gaactgccta ttttagcaga tgagtatgtt gatatagact
     9541 tcggttctgg tgctatgaaa gtgacaccag cacatgaccc taatgatttt gaaattggtc
     9601 aaagacatca attagaaaat attatcgtta tggatgaaaa tggtaaaatg aacgacaaag
     9661 ctggtaaata cgaaggtatg gaccgttttg attgtcgtaa acagctagtt gaagatttaa
     9721 aagaacaaga tttagttatc aagattgaag atcatgttca ttctgtaggt cattcagaac
     9781 gatctggcgc tgttgttgaa ccatatttat caacacaatg gtttgtgcgc atggaagact
     9841 tagcgaaacg ttcattagat aaccaaaaaa cagatgaccg tattgatttt tatccgcaac
     9901 gtttcgaaca tacatttaac caatggatgg aaaatattag agattggacg atttcaagac
     9961 aattatggtg gggtcatcaa attccggctt ggtatcataa agaaacaggc gaaatatatg
    10021 ttggagaaga agcgccaact gatattgaaa attggcaaca agatgaagat gtattagata
    10081 cgtggttctc aagtgcttta tggcctttct ctacgttagg ttggcctgat ttagaaagcg
    10141 aagactttaa acgatactac ccaacaaatg ccctagttac aggttacgat attatctttt
    10201 tctgggtagc acgcatgata ttccaaggct tagaatttac agatcgtcgt ccatttaatg
    10261 atgtgttatt acacggctta gttcgtgctg aagacgggcg taagatgagt aaatcattag
    10321 gtaatggtgt ggatccaatg gatgttattg acgaatacgg tgctgatagc ttgcgttact
    10381 tcttagcaac gggttcatct ccaggacatg atttaagata ctcaactgaa aaagttgagt
    10441 cagtgtggaa ctttatcaat aaaatctgga atggggcacg tttcagttta atgaatatcg
    10501 gtgaagactt taaagttgaa gatatcgatt taagtggtaa cttatcatta gcagataaat
    10561 ggattctaac acgtttaaat gaaacgattg caacagttac tgatctaagt gacaaatatg
    10621 aatttggcga agttggacgt gcattatata atttcatttg ggatgatttc tgtgattggt
    10681 acattgaaat gagtaaaatt ccaatgaatg gtaatgatga agaacaaaaa caaattacac
    10741 gttcagtatt gagttatact ttagacaata ttatgagaat gctacatcca ttcatgccat
    10801 ttgtaacaga gaaaatatgg caaagtttac cacatgaagg tgacacaatt gttaaagctt
    10861 catggccaga agtgcgtgaa tcattgattt ttgaagaaag taaacaaaca atgcaacaac
    10921 ttgttgaaat cattaaatct gtaagacaat cacgtgtaga agtaaatacg ccattgtcta
    10981 aagaaatacc tattttaatt caagctaaag ataaagaaat tgaaacaact ttatcacaaa
    11041 acaaagatta tttaatcaaa ttctgtaatc ctagtacgtt aaatattagc actgacgtgg
    11101 aaattcctga gaaagcaatg acatcagttg taattgcagg taaagtggta ttaccattag
    11161 aagggctaat tgatatggat aaggaaatca gccgtttgga aaaagaatta gctaaacttc
    11221 aaagcgaatt agatagagta gataaaaagc tctctaatga aaactttgta agtaaagcgc
    11281 ctgaaaaggt tataaatgaa gaaaaacgta aaaaacaaga ttatcaagaa aaatatgatg
    11341 gtgtgaaggc aagaattgaa caattaaaag cataggagtt agtaacaatg aattacctag
    11401 agagcttgta ttggatacac gaaagaacta aatttggcat caaaccaggt gttaaacgta
    11461 tggaatggat gctagcacaa tttaataatc ctcaaaataa cattaagggt attcatgtag
    11521 gtggcacaaa tggtaaaggc tctacagttg cttaccttag aacagcttta gttgaaaatg
    11581 gttatgaagt aggtacattt acgtcgccgt ttattgaaac atttaatgaa cgaattagtc
    11641 taaatggtgt gccaatatca aatgacgcta ttgtagaatt agtatcacgt attaaaccaa
    11701 ttagtgaaat gatggaacgt gaaacagatt taggtgttgc aactgaattc gaaataatca
    11761 cagcgatgat gtttttatat tttggtgaaa tacatcctgt tgattttgtc attgttgagg
    11821 ctggattggg tataaagaac gattcgacaa atgtctttac accgatttta tcaatcttaa
    11881 ctagtatcgg tctagaccat acagatattt taggtggtac ttatctagat attgctaggg
    11941 ataaaggcgc gattataaag cctaacgttc cagtgatata tgctgttaaa aatgaagatg
    12001 cattaaagta tgttcgtgaa cgcgcaattg aacaacatgc aaagccaatt gaattagata
    12061 gagaaattgt tgttgtatcg caaaatgatg aatttactta ccgttataaa gattatgaat
    12121 tagaaacaat cattttaagt atgttaggtg aacatcagaa acaaaatgct gcattagcca
    12181 taacagctct tattgaatta aatgaacaag gattaattga attagatttc aataagatga
    12241 tagacggtat tgaatcagtt cgttggactg gacgtattga gcaggtgcat gacaaacctt
    12301 taatcatttt ggatggcgca cataattcag agagtataga tgctctaatt gatacaatta
    12361 aacagtacca tgataaagaa aaagtagata ttttgttctc agcaataaac ggaaaaccga
    12421 ttaacgagat ggtcaaacat ttaagtttaa ttgcgcatac gttttatgca actgaatttg
    12481 attttccgaa agcgttacgc aaagaagaaa ttgtaggtag tattgaaaat gatgaaatac
    12541 aattagtaga tgactacgtt gaatttataa aaaattatca aggtgataca ttagtaatta
    12601 ccggtagtct gtacttcata agtgaagtta aatcaaaatt agactttgaa gcattataaa
    12661 actaaaatag ccatgaagaa tctatttaat caataggtag cttcatggtt attttttatg
    12721 ctctaaatcg tatgtgttta taaggttcaa tttcttgcca ttattaattt tgacgagcag
    12781 aaatgggtta ttaaagtatc catcaattat gcttagcaat attgaagaat ttaaatgaaa
    12841 aagaggagca attcttggta aatagtgtat atttacattt cggtaataac taactatact
    12901 ttacgtaagg aggttgtcat ttggtagtat tgttatctta tagttgcagt tgtatcttta
    12961 gttttctata tcaatttata tctatagaag aaacgtcatt tgattattta catagaagat
    13021 cgaaatgtga ttattgtaat tcatcactca aatggtatga attaatgccg attattagtt
    13081 ttttattatt aaaagggcga tgtcgaaact gtcgtaagcg tatttcccta acacatttct
    13141 taggggaaac ctttgcttta atacctatcg tctttattaa gtatgatttc acatacgtaa
    13201 atgctacgct atttataact acatatgttt ttctgcttat ttttactatg accgatatca
    13261 cttctttaat gttagattgt cgcttaatta taatttattg tatcgtttct ctttcgttaa
    13321 gtattattta tccagtagct tttatcatta ttagtatgac cacgcatata ttctactttt
    13381 tatttcgggc atatattggt tatggtgacg ttttactaat atctgcactt tctttgtttt
    13441 tccctctcca attcactatt tatgtcattt tatttacatt tgtcattgct ggtttagttg
    13501 ctttaattac catgatattt aagccgataa aactattacc ccttgttcca tttatattta
    13561 tttccttttt tatcaattca cttttttata atgatatcca tcaattttta ggaggcgtat
    13621 atttttgaaa attaaagaaa tggtaacttc agaaatgcca agagaacgtt tgttaagcca
    13681 tggtgcaaaa agtctttcga atacagaatt attagctata ttaattaaca ccggaagaaa
    13741 aggattctcg agtatagaca ttagtaatga actgcttaaa tctgcttcga atctgaatga
    13801 attgaaaaaa tcttcaatta acgatttgat acaagttaaa gggattggat tacaaaaagc
    13861 aattacatta aaagcagcat ttgagttagg ggaaaggatg gggagaagag ctgaaaataa
    13921 tcgtataaaa ataacgcaac caagtgatgt tgctgattat atgattccaa caatgaaaga
    13981 tttaacacaa gaacattttg tcattttatt gttaaattca aaaaatgtag tgattaaaga
    14041 aagctgtgtt tttaaaggta cattaaatag ttcgattgta catccacgcg aaatttttag
    14101 tattgcggtg agagaaaatg ccaatgcaat catcgcagtt cataatcatc catccggtga
    14161 tgtaacgccc tcacaagaag atatcttaac aacaatgagg ttgaaggagt gtggtttgat
    14221 tttagggata gatttattgg atcatattat aatcggtgat aatagattta cgagtcttgt
    14281 agaagcgggt tactttgatg aaaatgattg aagttaatag aattaatgac gtatcttgtg
    14341 tataatacct acgaagtact ttcattggag gaaaaatagt gactctattt attattatcg
    14401 gggttctcgt gccaatggtt tataccatgc agttaaatat taaaaatgaa cctgtaacaa
    14461 agcgcaatct attaataaca ttagctttat ctacggtagg tattttagta acggcgttag
    14521 caggtgtgat tgttacgaaa caagcatttc ctttattaag tgtagcaata ggctcaattt
    14581 ttactggaat cgtttggggc cttttactaa gtggtagcta cgcgctgata cgatttttat
    14641 ctaacgcatt tgggcgtaag taatagtaat ctgttcatca agtagtatcc gtgcttgaaa
    14701 acaaattaaa actcctaatg tggaactagt taaaaaattc taaactagta cattaggagt
    14761 tttgttatgc agaataaatt taattgttaa attgaaagtg tggtaaaaac ccactattta
    14821 tttgaaaaaa atcgagcaac catattaaac cttgatatcc taagtaaata cataataaaa
    14881 caagtccgac ataaattaaa aatcgcaaaa tagaaagtcc aactctaaaa aggaggatga
    14941 ctaatagtgc tattaaaata attgttagta tactcaatgc tacaaacctc ctcatacgct
    15001 tttaaatcat aacaaaatta taagctattt taaatgaacg tgcatcagtc ctaagatata
    15061 tcatgattaa ggctgaagac tgaggaaaat atacataata aaatatataa ttagatgaag
    15121 acattggagg cgaactatga gattgatatt tagtatgatt aaaaacatta tagcagtaat
    15181 agcaattgtg ataattgttt atatcgcctt gaaatacgca ccttttttaa aagaacaaga
    15241 ttggaatcct gtaaatcacg ataatgatca aacgaatcaa gttacgcaac ccactaatga
    15301 tgcccaacat gtttatgtaa gtggtgagaa atattcatta aaagaaaatg atttaattaa
    15361 gaatgtacca ttaagccaaa ttaaaaatgt atttaaaatg atagataaac aagagtttat
    15421 ggctgtgtct ggaatgaatc gcatggctta taatgatcaa tatattatag gtcaaagagg
    15481 agacgaattt attctttata aatttggaga tgagtcaatg cgtgtttaca atactgaatt
    15541 tgaaatgcaa caagacttaa atgaattagg gcaaaattta caactaaaac ctgaaaatgc
    15601 ttatcaatag gaaattattg cataagaaat atattttagt tatcctgtca ctagaataaa
    15661 tttaaaaggt tggtgtagtt caaatggcag aacaatcaaa acagaaacaa gctaatgaac
    15721 aacaaaaggc ccagaactta ttcgcacgtt ggagacaact tcagaattcg aacagtgaat
    15781 cctcgaacga tacaaacaga taattttaaa atttaaatcg attatttaaa ctactagtga
    15841 ctggaatgtt tgctagtagt ttttgttttt aaaatgccac aaatttttta cggagttgtt
    15901 cattttcaaa gtaattcgta gttagtgctt tatttcataa tgggagtaat ataaaataga
    15961 aaaggatata caatattttg acataataga ggtgttctgg gtgcttaagt tttttaaaaa
    16021 taacaaatta attgttgttt tatgtgcaat tatcgttttt attgcattaa ttgggctgtc
    16081 catacgttca caatctcaat cacctcctga acaatatata ggtgattctg tgtcctttgg
    16141 acaacgagtt gtgagttatc cagttaattt tgttgctggt acgattgggg acttttttaa
    16201 aaaaggagat tctaaagaat ctaaaaataa gattagccag ttagaatcta agaaccaaca
    16261 attagaagcg gaaaatgaaa aattaaaaaa agagcttgat ttaaaagata tttcaaaatt
    16321 tgatcctatt tctactacgg ttttagcaag aaatccggat cagtggatga atacaattgt
    16381 aattgataag ggatctaaat caggtataac ttcaaatatg gctgtgatga catcacaagg
    16441 ttttgttgga agagttacta aagttaataa attttcttca caagttgatt taatctcaac
    16501 taatacacgt gcgggtaaat tatctgtaaa tatacaacac ggttctaaaa atatatttgg
    16561 attaattgat cgttatgatg aaaagaactc agaacttgta attagtgaca ttaataatag
    16621 agataatatc tcaaaaggtg ataaagtcgt tacaagtgga ttagctgatc aactaccaag
    16681 taatttatat ataggagaag tgactaaggt tcaaaatgat caatacggct tagctaaaga
    16741 agttagggtt aagactggtg cagacttaac agatttgagt catgtttatg ttgcaaaaag
    16801 agatcctaaa acaattcctg atgatgaaag cagggataaa taatgcgtac actgtattat
    16861 tttttgatag gtattttact attttatata gatactgcta ttgggcttct tattccaatg
    16921 cacataggta agtttgaact tgtatttgta ccacacctta catttatgta tattttaatg
    16981 atggtagtgt atcgtggctt tggtgtatca ttattgctca gcatattttt aggcgtaatg
    17041 actgatgtat actttggtag tatttatgga gtgtacttat ttggctatat attgttttta
    17101 gcacttatag atcgattctt taaaattttt tacaaagatc attcgatgtt attcattatt
    17161 atattagcca gcaccttatt attagaagtc tatgtggcat taatatacgg tatgttagga
    17221 ttcattcaat ttgatattat tcattttgta gtctttagat tattgccaac attaattatg
    17281 aattttgtac tgttgattat gctttatccg ttgattataa agttccttaa aaaaacaaac
    17341 aatgacattg acatgaaacg ccgtcaatgg taagatgcaa tagttgagta gttataagta
    17401 gctacataca accgctcaaa tataggttca agaacatttt taatgaagaa agcgattgtc
    17461 aatcaaagcg ttgaaggaag aaacgagaag agtttatctt tataaatgac tcgagttgcg
    17521 accgaaacaa cgtagagtgc aaacgcttga ctcatttgca gaagttattg aaagaaagcg
    17581 attgtcaatc aaggcgttga aggaagaaac gagaagagtt tatctttata aatgactcga
    17641 gttgcgaccg aaacaacgta gagtgcaaac gcttgactca ataacgaaat gtcacctatg
    17701 tatggcgtga cttattatac aggaggtgca aagtatgttt gctattattg aaacaggtgg
    17761 aaaacaaatc aaagtagaag aaggtcaaga aatcttcgtt gaaaaattag acgtaaacga
    17821 aggagatact tttacatttg ataaagtatt atttgtaggt ggagattcag ttaaagttgg
    17881 agcgccaaca gttgaaggtg caacagttac tgctactgtt aataaacaag gtcgcggtaa
    17941 aaaaattact gtattcacat acaaacgtcg taaaaattca aaacgtaaaa aaggccatcg
    18001 tcaaccatac actaaattaa caatcgataa aatcaacgcg taattattat gattactgtt
    18061 gatattacag ttaatgatga aggcaaagta acagacgtta ttatggatgg ccatgctgac
    18121 catggtgaat atggtcatga tatcgtttgt gctggagctt cagctgtatt gtttggtagt
    18181 gttaatgcga ttataggatt gacatctgag agaccagata tcaattatga cgacaatggt
    18241 ggtcattttc atataagaag cgttgataca aataacgatg aagcgcaact aattcttcaa
    18301 acaatgcttg tgtctttaca aactattgaa gaagaatata atgagaatat tagattaaat
    18361 tataagtgag gtgcattccg atgttaaaat taaacttaca attcttcgca tctaaaaaag
    18421 gggtaagttc tacaaaaaac ggacgtgact ctgaatcaaa acgcttaggt gctaaacgtg
    18481 ctgacggtca attcgtaaca ggtggttcaa ttttatatcg ccaacgtggt actaaaattt
    18541 accctggtga aaatgtaggt cgtggtggcg atgatacatt attcgctaaa atcgacggcg
    18601 ttgttaaatt cgaacgtaaa ggtcgcgaca aaaaacaagt ttctgtatat gcagtagctg
    18661 aataattttg tctagttaac accagaagtg aatcttctgg tgttttttac tttttataaa
    18721 acgtttttca taattatttt cattatgtta ttaaaaaaat ggggatttta gacgttatac
    18781 taaatgtgca actgcataag agcctctaat tatttacaat atataagggg ggctaaggaa
    18841 tacagttgca gcgtttaaag aataaactgt accacaattg gtgctgagaa atataagtat
    18901 tttaaagcaa agatttataa aagcaactgc ataagagccc ctaattattt acattataag
    18961 gggctaaaag gaatacagtt gcagtataaa cagcaaaaaa gtatcatatt tggtacttgg
    19021 aaatatcagc aatttaagta aagtaaattt aaaagcaact gcataagagc ccctaattat
    19081 ttacaatata taaggggctc taatatgcta taattattgg gaaaatgaaa attatatgta
    19141 aaagaggtga gatatatgtt tgtcgatcaa gtcaaaatat ctcttaaagc cggtgatggt
    19201 ggtaatggta ttaccgcata cagaagagaa aaatatgtac catttggtgg accagctggc
    19261 ggtgacggtg gtaaaggtgc ttcagtcgta tttgaagtgg atgaaggttt aagaacgtta
    19321 ttagatttta gatatcaacg tcattttaaa gctagtaaag gtgaaaatgg ccaaagtagt
    19381 aatatgcatg gtaaaaatgc ggaagattta gtattaaaag ttccgcctgg tacaattatt
    19441 aaaaatgttg aaacagacga agtgttagca gatcttgttg aagatggtca aagagctgta
    19501 gtagcgaagg gcggtcgagg tggccgaggt aattcacgtt ttgcaacacc tagaaaccct
    19561 gcacctgact tcagtgaaaa aggcgaacca ggtaaggaat tagatgtatc tttagaattg
    19621 aaattattag ctgatgtagg attagtaggt ttccctagtg tgggtaaatc gactttatta
    19681 tctatcgttt caaaagctaa gcctaaaatt ggggcatatc attttacaac gattaaacca
    19741 aatctaggtg ttgtttcaac acctgatcaa cgaagttttg ttatggcaga tttaccaggt
    19801 ttaattgaag gtgcatctga tggcgttgga ttaggacatc aatttttaag acatgtagag
    19861 agaacaaaag ttattgttca catgattgat atgagtggtt ctgaaggtag agaacctatt
    19921 gaagattata aagtcattaa tcaagaatta gctgcgtacg agcaacgttt agaagataga
    19981 cctcaaatcg tagtagctaa caagatggat ttacctgaat cacaagataa tttaatcttg
    20041 tttaaagaag aaattggcga agatgtgcca gttattccag tttcaacaat aacgcgtgat
    20101 aatattgatc aattattata tgcaatagca gataaattag aagaatataa agatgttgac
    20161 ttcacagttg aagaagagga gtcggttggc attaaccgag tattatataa acatacaccg
    20221 tcacaagata aatttacaat ttcaagagat gatgatggtg cttatgtggt aagtggtaat
    20281 gctattgaaa gaatgtttaa aatgactgac tttaacagtg atccagcagt acgtcgattt
    20341 gctcgtcaaa tgcgttcgat gggtattgat gatgcgctta gagaacgtgg ttgtaaaaat
    20401 ggtgatatcg ttagaattct tggcggagaa tttgaattcg ttgaatagga gcgtaacatg
    20461 atggacaata aagattataa aaagttttat ttaattagag aagatgtctt gcctgaatcc
    20521 gtggttaaaa cattgaagat taaagatgcc ttaaaaagtg atccgacatt gtccatttat
    20581 gatgccgtta aacagtttga tctatctaga agtgcttttt ataaatatag agaaacgata
    20641 tttccagtag acgataaaat gcttgaccat cgagaattta cattaatttt atatgtaact
    20701 gatattgtgg gtatgttggc acgtgtacta gatgttatat caaagttaga actatctgta
    20761 ttaacgattc atcaaagtat tccattggaa gaaaaagcaa caataacatt atcactgaat
    20821 gctaaatcta aagaaacttc agtagaagat gttattggcg ctttgagaaa tttagattat
    20881 gtatcaaaag tagaattaat tagtatgagt atgtaaggat gtgcctataa tgtacgcgta
    20941 tgtcaaaggt aagttaacac atttatatcc tacacacgta gttgttgaaa ctgctggtgt
    21001 tggttatgaa attcaaacac caaattctta tcgttttcaa aagcatctag atcatgaagt
    21061 tttaattcat acatctttaa ttgttcgtga agatgcacaa ttattgtatg gatttagtag
    21121 tgaagaagag aaagatatgt tcttgagttt aattaaagtt actggtattg gtccgaaatc
    21181 agctttagct attttagcga caagtacgcc taatgaagta aaacgtgcca ttgaaaatga
    21241 aaatgatacg tatttaacta aattcccagg aattggtaag aaaacggcaa gacagattgt
    21301 cttagattta aaaggtaaag tgaaaatcac tgaagaagat agcgattcat tattacaagt
    21361 agacgctaca tcgacggtgc aagatcaatt cgtgcaagaa gcaatgttag cgttagaagc
    21421 attaggttat tctaaacgag agcttgcaaa agttgagaaa acgttaaata aaaataaata
    21481 tgactcagtt gatgaagctg ttaaggcagg tcttcaatta gttgtatctt aattttaaat
    21541 agattaatag gggaagtgtt gtcatgaatg agcgtatggt tgatcaatca atgcatagtg
    21601 aagaaactga tttcgaattg tcgcttagac ctacgagatt acgacaatat attggtcaaa
    21661 attcaataaa aagtaattta gaagtattta ttaaagcggc taaacttcgt catgaaccat
    21721 tagatcatgt attgcttttt ggcccccctg gattaggtaa gacaacatta tctaatatca
    21781 ttgccaatga aatggaagtt aatatacgta cagtatcagg gccttcatta gaaagacctg
    21841 gtgatttggc tgcaatttta tcagggcttc aacctggaga tgttttgttt attgatgaaa
    21901 tacacagact gagtagtgtt gttgaagaag tgttataccc tgcaatggaa gatttctttt
    21961 tagatattat cattggtaaa ggcgatgagg ctagaagtat ccgaatcgac ttacctccat
    22021 tcactttggt aggtgcaaca acgcgagctg gtagcttaac aggtccacta agggatcgat
    22081 ttggtgtgca cttaagatta gaatattata atgaatcaga tttaaaagaa atcattatta
    22141 gaacagctga ggttttgggc acaggtattg atgatgaaag tgctattgaa cttgctaaac
    22201 gttctagagg gactccaaga gttgcaaatc gactattgaa acgggtaaga gacttccagc
    22261 aagtgaatga agatgaacaa atatacattg aaacaacaaa gcacgcatta ggtttacttc
    22321 aagttgatca acacggacta gattacattg atcataaaat gatgaactgt attattaagc
    22381 agtataatgg tggacctgtt ggtttagata cgattgccgt aacaattggc gaagaacgta
    22441 ttacaattga ggacgtttat gagccatttc ttattcagaa aggcttttta gaacgtacgc
    22501 cacgtggcag aaaagcaaca ccattagctt atgaacattt tgcaaagtcg aatgaggaga
    22561 gagaataacg tgaatattga agaatttgac tatgacttac cagaatcatt aattgctcaa
    22621 acgcctttaa aagatcgtga tcatagtcgt ttattagtca tggatagaga aactggtgaa
    22681 atgaaacatt tacatttcaa agatatcatt gagtatttta gacctggtga tacattagtg
    22741 cttaacgata cgcgagtaat gccagctaga ctttttggtt tgaaagaaga aactggtgca
    22801 aaagttgaaa tgttaatgtt aacccaaatt gaaggtaatg attgggaagt cttactgaaa
    22861 ccagctaagc gtattaaagt tggtaataaa ttgaattttg gtaatggcaa aattatagct
    22921 gaatgcataa aagaaatgga tcaaggtgga cgcatcatgc gtttacatta tgaaggtatt
    22981 ttacaagaaa gattagatga attaggggaa atgccactgc caccatacat caaagaacgt
    23041 ttagatgatc cagatcgtta tcaaacagtt tacgctaaag aaagtggttc agcggcagca
    23101 ccaacagcag gattacattt tactgatgag ttattaactg aaattaaaaa taaaggtgtt
    23161 aatatcgcat ttgttacatt acatgttgga ttaggtacgt ttagaccggt gagcgtcgac
    23221 gatgtgaatg accacgaaat gcatagtgaa tattatcaaa tgacacaaga aacagctgat
    23281 ttattaaatg atactaagtc caaaggacat cgtattatat cagttggtac aacttcaaca
    23341 cgtacacttg aaacaattcg acgcgatcat gataaatttg ttgaaacgag tggctggact
    23401 aatatattta tttatccagg atttgatttt aaagcaattg atggccagat tactaatttt
    23461 catttaccaa aatcaacatt agttatgcta gtatcagcgt ttagtactcg tgaaaatgtt
    23521 ctgaatgctt ataaaacggc agtaaattta gaatatagat tctttagttt tggcgatgca
    23581 atgttaatta tataaaaaga atgtgaggat tttgaatatg cctgcagtaa catacgaaca
    23641 cattaaaact tgtaaacaat caggtgcgcg tttaggtatc gtgcacacac cacacggttc
    23701 atttgaaaca cctatgttta tgccagttgg tactaaagca accgttaaaa caatgagtcc
    23761 agaagagtta agacaaattg aagcaaaaat cattttgggc aacacatatc atttgtggtt
    23821 acaacccgga aatgatatta tcaaacacgc tgggggatta cataaattca tgaattggga
    23881 tggtccgatt ctaacagatt caggcggttt ccaagtgttt agtttaagta atttacgtaa
    23941 aattacagaa gaaggcgtgg aatttagaca tcatactaat gggtctaaat tatttttgag
    24001 tcctgagaaa tcaatgcaaa ttcaaaatga tttaggttct gatattatga tggcatttga
    24061 tgaatgtcca ccgatgcctg ctgaatatga ttatgtaaaa aaatctattg aacgtacaac
    24121 acgttgggcg aaaagatgtc tagatgcaca ccaaagacct gaagatcaag cattgttcgg
    24181 cattatacaa ggtggcgaat atgaagattt aagagaacaa agtgcaaagg atttagtaga
    24241 attagatttt cctggttatg caatcggcgg tttgtcagtt ggtgaaccta aaccggttat
    24301 gtataaaatg gttgaacata cagagcagtt tatgcctaaa gataaaccaa gatatttaat
    24361 gggtgtagga tctccagatg cgttaatcga atgtagtatt cgcggcatgg atatgtttga
    24421 ttgtgtctta ccgacacgta ttgccagaaa tggtacttgt atgacatcgc aaggtcgttt
    24481 ggttattaaa aatgcaaaat ttgtagatga tttaagaccg ttagatgaga attgtgactg
    24541 ttatacatgt caaaactact caagagcgta tatacgtcat ttaatcaagg cagaggaaac
    24601 ttttggtatt cgtcttacta ctattcataa tttacatttt ctgctaaaat taatggaaga
    24661 tataagacaa gccattcgag aagatcgtct tttagatttc aaagaagaat tcttcgagca
    24721 atatggatta aatgttgaga acccaaaaaa cttttaagca agaggagcgt ataaaatgca
    24781 attttcatta ctaatatata tagtcgtaat ttttgcggtt atgtatttct tgatgatcag
    24841 accacaacaa aaacgtgcga aacagcatcg tgagttgatt aataacattc aatctggtca
    24901 aagaattaca actattggtg gtattaaagg tactgttaaa gcagtagatg aaacaactgt
    24961 tgttattaca gttaatggtc atggtactga attaactttc gaaaaacctg ctattaaaca
    25021 agttgaccct tcataacaag aaatggtatt aaaaaggatg aaacgaatag cgtttcatcc
    25081 tttttaagtt ataagtagta tgtagaatag cttattttta ttccaaaaaa agaaattgtg
    25141 atacataata attgttaaat ttaaattaaa tatatctatg actattttta ct
//
LOCUS       DAHHRR010000028        24502 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.028, whole genome shotgun sequence.
ACCESSION   DAHHRR010000028 DAHHRR010000000
VERSION     DAHHRR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24502
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.028"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            61..513
                     /locus_tag="NBI42_000972"
     CDS             61..513
                     /locus_tag="NBI42_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001803582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777406.1"
                     /translation="MLFRQQMICACLLANVRHSSIVCLNNTCRIVDMRCLNTKIGGID
                     VSETDEPQGFEHTHNILNINQNSKSLMTFITSKLKSKLKQRIIIARGKKRIDYRLSYN
                     FYTTYYDNVEIKKIDCEYTHAMPIAILIRHTMSFDNAHFFAQLRHPVL"
     gene            733..963
                     /locus_tag="NBI42_000973"
     CDS             733..963
                     /locus_tag="NBI42_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCD1777407.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(1094..1471)
                     /locus_tag="NBI42_000974"
     CDS             complement(1094..1471)
                     /locus_tag="NBI42_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1777408.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     RTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNKEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(1488..2309)
                     /locus_tag="NBI42_000975"
     CDS             complement(1488..2309)
                     /locus_tag="NBI42_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HCD1777409.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDSVSLQLAMKGKYGLYSIQPIVKDDVNEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQDYNK"
     gene            complement(2328..2627)
                     /locus_tag="NBI42_000976"
     CDS             complement(2328..2627)
                     /locus_tag="NBI42_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HCD1777410.1"
                     /translation="MSLNKEQRRITAEELQAHFEESTLSVQMIAEKLNVTTEDVEKAL
                     AMKAPLGIFSHQLQRFIHSVWDVRDVINNNIKENGQTPEPYTYLKGEKEDYWFLR"
     gene            2758..3315
                     /locus_tag="NBI42_000977"
     CDS             2758..3315
                     /locus_tag="NBI42_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1777411.1"
                     /translation="MRKDAIENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCFYVIQTDLNTFITRFKQIKDDYHSDYEVMKVSLDYLLQFKIDNKTLL
                     HCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDIFTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVERND"
     gene            3308..4012
                     /locus_tag="NBI42_000978"
     CDS             3308..4012
                     /locus_tag="NBI42_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD1777412.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNSETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNAKHVSEGIKPIIKLATIDE
                     NGPTGTFINGNGEMPW"
     gene            4110..5120
                     /locus_tag="NBI42_000979"
     CDS             4110..5120
                     /locus_tag="NBI42_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1777413.1"
                     /translation="MKSITFEEHYVIDEIQKETMNAITADPNGVPMKVMLDGLEKKTG
                     FTNADELSNHDKRIQFMDNQNVQIQVLSYGNGSPSNLVGQKAIELCQKANNQLADYIS
                     QHPDRFVGFATLPINEPEAAAQEFERCVNELGFKGALIMGRTQDSFLDQDKYDVIFKT
                     AEALEVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNIIIGHWGEFIPFFLERMDEALLADHLNHPISYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSS
                     NN"
     gene            5139..5968
                     /locus_tag="NBI42_000980"
                     /pseudo
     CDS             5139..5968
                     /locus_tag="NBI42_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(6116..7006)
                     /locus_tag="NBI42_000981"
     CDS             complement(6116..7006)
                     /locus_tag="NBI42_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD1777414.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVVFVNKIDSAH
                     VDTTSKLLKDLEVINPDADIQVGMHGRVALPISVSQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAQFIKRLACLPSHIYRLKGFMTFEDTTHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(7107..8447)
                     /locus_tag="NBI42_000982"
     CDS             complement(7107..8447)
                     /locus_tag="NBI42_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCD1777415.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAVIDQTGLKSWMIWAIEPSMLRIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSFISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIHQMLLQMWQNIQMFIVQALPIFITICLIVSVLSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLQQGALLQGMTAIQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGKSALKLIGKQMVTSLSLVIGVGLIVKMIMMII"
     gene            complement(8441..9547)
                     /locus_tag="NBI42_000983"
     CDS             complement(8441..9547)
                     /locus_tag="NBI42_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCD1777416.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGIEDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTETYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNDGQYHISFDSGQSVHTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            complement(9883..10179)
                     /locus_tag="NBI42_000984"
     CDS             complement(9883..10179)
                     /locus_tag="NBI42_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HCD1777417.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFDNMKQNLDSEIQRGEVTQQ"
     gene            complement(10167..10535)
                     /locus_tag="NBI42_000985"
     CDS             complement(10167..10535)
                     /locus_tag="NBI42_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777418.1"
                     /translation="MSNKLDGINKMITAKHKQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKAEEEMNDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            complement(10550..10828)
                     /locus_tag="NBI42_000986"
     CDS             complement(10550..10828)
                     /locus_tag="NBI42_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCD1777419.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLISKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            complement(11165..12031)
                     /locus_tag="NBI42_000987"
     CDS             complement(11165..12031)
                     /locus_tag="NBI42_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCD1777420.1"
                     /translation="MNEQWLEHLPLKDIKEISRVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNKFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRMEHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTG"
     gene            12249..13313
                     /locus_tag="NBI42_000988"
     CDS             12249..13313
                     /locus_tag="NBI42_000988"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCD1777421.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTTFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSIEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            13468..13743
                     /locus_tag="NBI42_000989"
     CDS             13468..13743
                     /locus_tag="NBI42_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777422.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERKGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(13929..14651)
                     /locus_tag="NBI42_000990"
     CDS             complement(13929..14651)
                     /locus_tag="NBI42_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCD1777423.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTKYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            15002..15451
                     /locus_tag="NBI42_000991"
     CDS             15002..15451
                     /locus_tag="NBI42_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HCD1777424.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGIFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(15538..16125)
                     /locus_tag="NBI42_000992"
     CDS             complement(15538..16125)
                     /locus_tag="NBI42_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1777425.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTYYYDKYDLLEKLEDQQLEEIRNFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IVFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSLI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(16103..16211)
                     /locus_tag="NBI42_000993"
                     /pseudo
     CDS             complement(16103..16211)
                     /locus_tag="NBI42_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16268..17950
                     /locus_tag="NBI42_000994"
     CDS             16268..17950
                     /locus_tag="NBI42_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCD1777426.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGPLWPTLGTIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVSFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSEIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNTEYTPLYLNAENHTLNHAKISSAHVAQYDSLDKQQD
                     IRFKYTFDKDTELVGNMNLKLWVRTTDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSTIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVIVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPVIN"
     gene            complement(18060..18695)
                     /locus_tag="NBI42_000995"
     CDS             complement(18060..18695)
                     /locus_tag="NBI42_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1777427.1"
                     /translation="MKKVISLFVAILSVLIFTDNANAENNKPEGVNENSNLIPVRQPD
                     ANYPGPVSDIARVKKLDNLIDGTKDPDVIKQLKDDNEVQNNAVKANGSKIVSDCRHLL
                     KHPDNLIDGTKDPNVINQLKNGNVTSNNGAVNPSVKANDTHTKSNKQIAKQQSNHWDS
                     KSKSGHKQQLKELPKTGENNESNTAIMALFTGLTLLVVSFMTRFLKLNSHQ"
     gene            complement(19022..19405)
                     /locus_tag="NBI42_000996"
     CDS             complement(19022..19405)
                     /locus_tag="NBI42_000996"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCD1777428.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGKRGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKHHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(19407..20258)
                     /locus_tag="NBI42_000997"
     CDS             complement(19407..20258)
                     /locus_tag="NBI42_000997"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCD1777429.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITVVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEKERQEAVHLSKSLQLAQALYQDGE
                     RQSKVIIDRVTQYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(20251..21069)
                     /gene="panB"
                     /locus_tag="NBI42_000998"
     CDS             complement(20251..21069)
                     /gene="panB"
                     /locus_tag="NBI42_000998"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCD1777430.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            21142..22002
                     /locus_tag="NBI42_000999"
     CDS             21142..22002
                     /locus_tag="NBI42_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD1777431.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HIPFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYQRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(22088..22792)
                     /gene="budA"
                     /locus_tag="NBI42_001000"
     CDS             complement(22088..22792)
                     /gene="budA"
                     /locus_tag="NBI42_001000"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCD1777432.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(23070..24029)
                     /locus_tag="NBI42_001001"
     CDS             complement(23070..24029)
                     /locus_tag="NBI42_001001"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCD1777433.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
BASE COUNT         7909 a         4566 c         3748 g         8279 t
ORIGIN      
        1 agggatttat gtcccagact catttttgtt tttgcgtact taaacaacac ttcaggcaat
       61 atgttgttta ggcaacaaat gatatgtgcg tgtttattgg caaacgtacg acatagtagt
      121 atagtatgtc taaacaacac atgtcgcata gttgatatgc gttgtttaaa tactaagata
      181 ggagggattg acgtgagcga gacagatgaa cctcaggggt ttgaacacac gcataatata
      241 ttaaatatta atcagaatag taagagtcta atgacattca ttacaagtaa attgaagtcg
      301 aagttgaagc aacgcataat aattgctcgt ggtaaaaagc gaatcgacta tcgactgtcg
      361 tataactttt acactacgta ttatgataat gtagaaatca agaaaatcga ctgtgaatat
      421 acccatgcta tgcccattgc aattttaata agacacacga tgtcattcga caatgctcat
      481 ttctttgctc agctacgtca tcctgtctta taaaacaaca ttgcagacat gtatatcaaa
      541 cgacacttca ataacatcac tttgccatcg tactactatt aaaatcgtgt ctcaaacccc
      601 ttattttaat tccaaaaatc tgctggtcaa aagaccgaga aactaaaaac attactgaat
      661 gtgttgataa attaccttat agaaataatc tcaaaatata tcaacacttg attctaagga
      721 ggatatgaca atatgaaaat tttagataga attaatgaac ttgcaaataa agaaaaagta
      781 caaccactta ctgtagctga aaaacaagaa caacatgcat tgcgtcaaga ctacttaagc
      841 atgatccgag gacaagtatt aacaacattt tccacaataa aagtggttga tccaatcggt
      901 caagatgtca caccagataa agtttatgat cttcgccaac aatacggtta tattcaaaat
      961 taatatttgc tcacgaggta ttgcacttaa ggtgccaact gacctcataa acaaagccca
     1021 tactgattga agacactaat gtgtcgcacc atctctgtat gggcttttta tttattcttt
     1081 tgagaatttc attttagcag accaaaaaat taaaatggtg tccatcaaga tctttgaaca
     1141 ttgcaccata aaacccttgg cttactgttg gttcttgaac gactgtgcca cccgcttctt
     1201 tcactttatt caccaactgg tcaacttctt tatttgtgtt cacacctaga gaaatcatca
     1261 cttcatttgg ttcagtatta aggctttgct gacttacatt ttgaaaatgc ttgttttcta
     1321 ttaaaattac ggttgttcga cctatttgaa tgccgaccat tttatctaac atttgtgggt
     1381 ttctatttat tttaaatcct aacgctttat aaaactgtgc gctcttttct aaatcttgaa
     1441 catgcaaatt aaaccacatt gattgaatca tgattgcacc ccattcatta cttattatag
     1501 tcttggactt taagccaatc acttaatgat aatcttgttg gatttatttc agccattaat
     1561 tcaaagtcta cttcataacc tttttcttcc aaccattgct tttctgcaac accactaaca
     1621 aattctcctt ctataacagt cgatttacct gtcacttcac taaaaattgt tgctgcttca
     1681 cttaatgtaa cttcatcgga accaatctct attgattgat gcgtaaagct ttgtggatgc
     1741 gcaaaaatat acgatgcaat tttagctata tcaatagaag aaatcattgt gaattttata
     1801 ttcggattaa taaattctgg taatgtaata cgttcatctt cgactttagc aatgcgtaaa
     1861 aaattatcca taaagaatga tggtttgata actgttgcat ttatattaga ttccattaat
     1921 ctattttcta tttttgctag tacttcaaag tgtgggccag ttcgatttcg attaacccct
     1981 cctgcagtac tatacacaat atgttgaata ttttcttgct cagctatttc aattatcttc
     2041 ataccttgtc ttaattcttc gttaacatca tctttaacga ttggctgaat actgtataag
     2101 ccatacttcc ctttcatcgc taattgcaaa ctaacactat cactcagatc accttcaacg
     2161 attgataaat gcggatgtcc tatgtctgaa agtttacgat tattcttatt tctagttaat
     2221 gcacttacat accatccatc ctctaacaac tgttttacaa ctgcattacc ttgcttccct
     2281 gttgcgccta ttactaaaat atctttcatt ttagatccac tcccctttta tcttaaaaac
     2341 caatagtcct ctttttcacc ttttaaatac gtatagggtt ctggtgtttg tccgttttcc
     2401 ttaatgttgt tattaatcac atctctaaca tcccatactg aatgaataaa tcgttgtaat
     2461 tgatgactaa aaatgcctag tggtgctttc atagctaatg ctttttccac atcttctgta
     2521 gtaacattca gtttttctgc aatcatttga accgataagg tagattcttc aaaatgtgct
     2581 tgcaattctt cagctgtgat gcgtctttgc tctttattta aagacattat caacactctt
     2641 ttcttttatg atatttaata gttattgcaa attcattgct aactttctta acacgtttta
     2701 taataagcgg agaattatcc gtttgtcaat tttagaaaac tataagtagg tggaacaatg
     2761 cgaaaggatg ccatagagaa taggcaacga attgaagaaa tagcgcacaa actttttgat
     2821 gaagaaggcg ttgaaaacat tagtatgaat cgtattgcta aagaactagg tatcggtatg
     2881 gggacactat atcgacattt taaagataaa agcgatttat gcttctacgt catacaaaca
     2941 gatcttaata cttttataac tcgatttaaa caaattaagg atgactatca ttctgattat
     3001 gaggttatga aagtatcttt agattatctt ttacaattta aaatcgataa taaaacactt
     3061 cttcattgta ttgaagctgg caacaataaa ttacgttttt accaaagcgc attttatcaa
     3121 gaattatttg atttttatta tgatttattt aaaagcgatg atgacatttt cacaaagttc
     3181 aaaactgata tgctgttgca atcattatca actagtgtat ttgccttcca aatagaacat
     3241 cgccatattt caattgaagc gtacagaaat tatttattaa acatttattt agatgaggtg
     3301 gaacgtaatg actaaaattg tgttaatcac aggaggcaat aaagggttag gctatgcaag
     3361 tgcagaggca cttaaagcat tgggttacaa agtttatata ggttctcgta atgacgttag
     3421 aggacaacaa gcatcacaaa aattaggtgt tcattatgta cagttagatg ttacaagtga
     3481 ctactcagtc aaaaatgctt ataacatgat tgctgaaaaa gaaggtcgtc ttgatatcct
     3541 cattaacaat gcaggtatat caggtcaatt ttcagcacct tccaaattga cgcctcgtga
     3601 cgttgaagaa gtatatcaaa cgaatgtatt cggtatcgta cgaatgatga atacattcgt
     3661 ccctctttta gaaaaatctg aacaacctgt tgttgttaac gtatcaagtg gtttaggatc
     3721 atttggaatg gttacaaatt ccgaaacagc tgaatctaaa gtaaattcgt tggcttattg
     3781 ttcatctaaa tcagcagtaa caatgttgac tttacaatat gccaaagggt taccgaatat
     3841 gcagattaat gctgcagatc caggtgctac aaatactgat ttggttggtg atttcagtaa
     3901 taatgctaaa catgtttcgg aaggtattaa acctatcatt aaattagcaa ctattgatga
     3961 aaatggccca actggcacat ttattaatgg taatggcgaa atgccttggt agattgaaca
     4021 attgttataa aaatctatca aagggtattg aaaaatgctt tagatactta tatgcaatta
     4081 tcatacaaaa cgctatggag gggaaataaa tgaaaagtat cacattcgaa gaacattatg
     4141 ttattgatga aatccaaaaa gagacaatga atgcaatcac agcagaccct aatggcgtac
     4201 ctatgaaagt aatgttggat ggactcgaga aaaaaactgg ctttacaaat gccgatgaat
     4261 tatccaatca tgataaacgc attcagttta tggataatca aaatgttcaa attcaagtct
     4321 tatcttatgg aaatggatct ccttcaaatt tagttggtca gaaagcaatt gaattatgtc
     4381 aaaaagccaa taatcaatta gctgattaca tttcacaaca tccagatcgt ttcgtaggct
     4441 ttgcgacact acctatcaat gagcctgaag ctgcagctca agaattcgaa cgctgtgtaa
     4501 atgagttagg atttaaaggt gcgctcatta tgggacgtac acaagacagt tttcttgatc
     4561 aagacaaata tgatgttatc ttcaaaacag ctgaagcttt agaggtacca atttatttac
     4621 accctgcacc agtcaatagc gatatttatc aatcttatta taaaggtaat tatcctgaag
     4681 taactgctgc aacatttgct tgttttggct atgggtggca tattgatgtt ggcatccacg
     4741 caatacattt agtattatct ggtatttttg accgttaccc taaattaaat atcatcattg
     4801 gacattgggg agaatttatc ccattcttcc tagagcgcat ggatgaggct ttacttgctg
     4861 atcacttaaa tcaccctatc agctattatt ttaaaaataa cttttatatt acaccgagtg
     4921 gtatgttaac gaaaccgcaa tttgatcttg ttaaaaaaga agtaggtatt gatagaatac
     4981 tttatgccgc tgattatcca tatattgaac ctgaaaaact cggtgtcttt ttagatgaac
     5041 ttggtttaac tgatgaagaa aaagagaaaa taagttatac aaatggtgct aaattattag
     5101 gtttatcatc taacaattaa caaatggagg tcattgatat ggaaacgtta gaattacaag
     5161 gtgctaaatt acgataccac caagttggac aagtcccgta ctgatcctta taccgggtgc
     5221 aaatggtacc ggagacattt ttctacctct tgcagaacag ttgaaaaatc attttactgt
     5281 tgtagccgtt gatcgtcgtg attatggtga gagcgagtta actgaaccac ttcctgattc
     5341 agcagcaagc cctgatagtg attatcgcgt taaacgtgat gcaaaagaca ttgctgaact
     5401 tgcgaagtca ctaagtgatg aacctgtgta tgtattaggc tcaagctcag gttccattgt
     5461 tgctatgcat gtattaaaag attaccctga agtcgttaaa aaaattgctt tccacgaacc
     5521 acctatcaat acatttttac cggatagtac ttattggaaa gataaaaatg atgaaattgt
     5581 tcatcaaatt ttgacagcag gattagaaaa aggtatgaaa acttttggtg aaactttaaa
     5641 cattgcaccg attgatgcaa aaatgatgtc tcaacctgcc gacacagaag aaggacgaaa
     5701 agaacaatat aaacgtacaa tgttctggtc agaatttgaa attcgtcagt acactcattc
     5761 cgacattacg ttagatgatt tcaaaaaata tagcgataaa atcacgttac tgaatggcac
     5821 agattcaaaa ggatcattcc ctcaagatgt taacttctat atcaataaag aaactggaat
     5881 acctattgta gatatcccag gcggtcattt aggttatatt caaaaacctg aaggctttgc
     5941 tgacgtatta ttaaatatgt ggggttaatc tatagggtct ctgataaaac agcttggaca
     6001 gttttcaaat tatatttgat ggctgaccaa gcttattttt gtttacgttt aaacgttcaa
     6061 atcaactcgt tattatacat gaaggcatgt taaccattct cttccactga aaacactact
     6121 gttccaaaca ttgatagtct tcttttgaaa taccctttcc gataagcacc aaatattctg
     6181 gcactttttt tgaaaatgct acaggtgtta attcatattg tccttgtgta aattgaatga
     6241 gatacgtatg tgtggtgtct tcaaatgtca taaacccttt caacctataa atatgagacg
     6301 gaaggcatgc taaacgtttt ataaactgtg ctttagtaca tttgactggt gattgcacaa
     6361 attgatgatt aatcatttga tgtaacgact tatgtttatt gtcagaagtt gctgtcattt
     6421 ggctaactga tattggcaaa gcgactctac cgtgcatacc aacttgtata tctgcatctg
     6481 gattaataac ttctaagtct tttaaaagtt tactcgttgt atcaacatgt gctgaatcta
     6541 ttttatttac aaacactaca gaacaatatg ctaattgctc ataaaataag ccttggatgt
     6601 ctttagggaa tgatttaatg tgtttataca tgcttgcgtc tattacacca atcatatgtg
     6661 taattgttgt aaacggagct aaaataggcg ttaaacaagc atctaaaaca gagaccggtt
     6721 ctgcaatccc actacattca ataaatacaa tgtctggttg ctcttttaaa tataattgat
     6781 gtaactgttc tgatacatct gctttcatgg cacaacagat acacccttct gtcagttcac
     6841 ttaaaggtat gtcttcagat actaaggcac catcaacact cattttgcca aattcattca
     6901 tgataactgc cggtttcagc gattccttta atgattcagc aagcaaatga tttaagacag
     6961 tcgttttacc gccacctaaa aacccaccta taataactat tttcatctgc gactctcctc
     7021 ttatacttca tagtgaatag aatattccaa gataaatttt ctattgactg acaagattct
     7081 ataatcagtt atagttcatt tcttttttaa ataatcatca ttatcatttt aacaatgagg
     7141 cctacaccta taactaaaga caatgatgtc accatttgtt ttccaatcaa tttaagcgcc
     7201 gatttaccac ctaaatgttt caaaagcatc gtcatcgtga ctgaacatgc tgtaaatgtt
     7261 gaactaaaaa acacaagcag tagtaactgt attgctgtca ttccttgtaa taaggcgccc
     7321 tgttgcaaat taaacaagag catgccgtct tttcgaatca ttgaaaataa aatccctggt
     7381 gacaattctg acgagatgcc taataacgat aatataggtg taaatatttg tgataaaaca
     7441 ttcaaaattg gcgttagtga taaaacacta acaataagac agattgtgat aaaaataggt
     7501 agcgcttgaa cgataaacat ttgtatattt tgccacattt gtagcaacat ttgatgtata
     7561 tttggcatat gtaattgcct atcataaggt agcggaacgc taagttgttg atcattcttt
     7621 ttcaaccata tcctattatg taagatgcca cctaaaagta ctaatattaa gtacggcata
     7681 aatagccatg actttccagc tacactaaaa atagataatg tcgcacctat ttgataacta
     7741 caagaactac caaagcttat gaaactcata cactgtgtct tcgtgcaggt atggcattgg
     7801 tgtgctgctt gtgaaatagc tgctgcatta catccaaatc cttctaatag tggcacgata
     7861 tcattacctt gtaatcctat ccgtaacatt gaaggttcaa ttgcccatat catccatgat
     7921 ttaagtcctg tttgatcaat tacagcagtt gataagctaa tcaatattac gaccggcaat
     7981 gcccatacga gcgaatatgt tccaagtgat agcacaccat aatcaccaaa taatatatgc
     8041 tgtaatatat cgtgattgag caatgttgtt tgagttaaat attctatcca tgctgataca
     8101 tactgatttt gaaaccaatc tgaaaattga taagcgatac cgattggtaa tgcaaacatt
     8161 aaaaatatca acaaccaggc taaaaatgtc gcacttattt taggtcttaa atgtcgtcgc
     8221 tcatgttgaa atagtgtcaa ttgttctatc acatgttcgc tcgctatagg cgaagaaact
     8281 acactttctg ccaatgaaat atctaatttc tgttttatga ttaataatct taatttcgct
     8341 ttttctgttg cggtaagttt atccgcatga ctaataacca aataatgagg ttcattaggt
     8401 atgggcgtat aatgccccgt tctcatatca tatttcactt ctaacatgtg catgacactt
     8461 cacaacatga ataatcatct aaatacattt gatttttttg gtaattttca atgacatctt
     8521 gtttagctgg taagccttcc cgctgtgtta aaagatgtgc aagtactgca aatcgcgctc
     8581 tgaatttata gatatagcat aatttggcat tatcattttc aactgtcgca ccaatcataa
     8641 aaatattcgg ataacgtgtc gattcatcat gtgttgttaa tttaatatct tgatttgttg
     8701 tcacaaataa ttgttgaacg attggatttt ttgttgcatc aaagccagtt gctagtattg
     8761 gttcatgagg cgtatgcaca ctttgtccgc tatcaaaact gatatgatac tgtccatcgt
     8821 taaaatcaat atctttaact gtataatgta cattcatttc aatgcgagct ccttgcttaa
     8881 tgacattacc tagtcgctga cgtgtataag gtgacaatct aacactagga tcagcatccg
     8941 gatcatttaa accggttgtg ctagtataaa gtgcgatgtc agagccattt tttgcaagtt
     9001 gatatgcagc atcaaaacca ctttcattac ctccgataac cacatattgt cccttattaa
     9061 agttatcaaa gtcttcaatt tcactataat gaataccata tttaaatggc tttttaggga
     9121 aattataatc acctgttgcg acaaagatat aatccgcgtg atatgtctct gttgtcgttg
     9181 caatcgtata atatgcatca tctacagata tatttgtgac aactgtattt tcaaagatat
     9241 tcagttcgta atggttggca accacttgta aatattcagc atatgtctct ccggaaatat
     9301 gttcttcatt aaatgtaaat gctggtgaag tatccatgga aattgcattc atatcaggca
     9361 tgccaaatcc attagacgta aatgacggcg taatcgtacg cgtcgattta ggccaatgtt
     9421 taaatgaatg gcctactgtt cctttttcta aaataatgac atcttctata ccgaaatctt
     9481 ttaaggtaat ggccatacct atacctgcag caccggcacc gataatagcc actttatgat
     9541 gttgcatatt aacactccct caatgatcta tttcaagtta ttgtttttga ttataaatcg
     9601 taataattac gatttataaa gtatagacct atccttgaga aatttcaata ttaaaagaaa
     9661 aacttccgtt attgtagaga attcatctac gtttcggaag ttggtatgtg tcttttttat
     9721 taaatttaaa gatgctacgg ttagttcaaa tgattgcgat agacattcaa tggttgtcat
     9781 ccagcctcag tacttggaga taacattttg gcggtgagac agaaatcata tttttacaaa
     9841 atttatttcg tcgtcccacc ccaataaaga aaaagtattt ccttattgtt gtgtaacttc
     9901 tccacgttgt atttcactat ccaaattttg tttcatatta tcaaacaact cttgaaatct
     9961 aacctcatct tgatcagaat aacctttaaa aataactgaa tctatatttt cctcgttaaa
    10021 ataataaatt tcatcttcca caacaccaat cggatattta catgcagaat agtcaaacat
    10081 atacttttga ttttcaattt ctacaattgg tcctctatta ataatcatta acttttgcga
    10141 accttctttt aaataaataa tgcttcctat tgtttccatt tattttcctc ctttgtcacc
    10201 gtctcaattt caacattcct tcgcatttcc aagtcattca tctcttcttc cgctttacga
    10261 atgccatgtt gaatttgctg ttgctgctca tataacgctt ccgttgaacg tcgtttcgca
    10321 aaatgaaatt catcgcggaa ctgttccata cgactagcca tattgctact ataataacgc
    10381 tcacctaaat gttgatataa ttgatctatc gaatgattaa gcgcatcact ttcatctatc
    10441 aatgctttaa cctctcgctt ttcatcatat aagtcatcca tttgcttatg tttcgctgtg
    10501 atcattttat tgattccatc aagtttattc gacatcattt caccacgttt taatttttac
    10561 caatttcatt accaatcatt acgtcagttt ggtcaaaagc ctcaccaact ttttgtatat
    10621 tatcgctcgc agttcttatt gcttcagcaa cagattgatt cttactaatc aaatcatcta
    10681 tcacactatg agcattatta tttccactta ctgttgtact ttcatcttta tccggtttcg
    10741 aaacattatt acatgtttca acagcgcttt taatagatga tgatgcacta ttggtcttaa
    10801 aaatatcact ttgaactgta ctcatcattt acacctcttc ccatttaaat tattttaaaa
    10861 ttattagaaa ctgtatcaat atatgatatt caaacattca attatactta aatattgttt
    10921 tgtgattata ttcaaaatta ttgtatcaat aaatatgtat catttctaga attttattga
    10981 acaaaaagtt agaaaataac ttttattgcg actcaatctt gttttgcatt atagaaatta
    11041 acaatagaga ttaaaatttt attttctaac aataaaaaag cacaaatatt tttattgagg
    11101 cagctgaagg ccttctcatt ataaaaatat ctgtgcatat tattatctca atctaacgta
    11161 ataactaacc tgttgtatct tgtaaaatct tatccataga gtgcgcaaca ctatcacggt
    11221 acatctcccc aaatttcaat aaatgcacca tcaataaata taaacgataa aattcaagtc
    11281 tataggatgc accttttgcg agtggataat gtttattata cgcatcataa aattcgctcg
    11341 taaaaccacc aaataccgtt gtaataccga tatcaaattc tctgtcacca tatagtggtg
    11401 ctggatcaaa taacgccgga cgaccatctt gtaagaacat ataattacca ccccatagat
    11461 caccatgtaa taaagatggt ttactttgat gcttttctaa ttccgccaca atttgacgtc
    11521 gcactttgtc atatactttg atatcgttgg catcccatag ccctctattt agcaactcat
    11581 ctttcaaatg ctccatgcgc ttgtcgacaa aaatggtaca ccaatcgtct tgccaatgat
    11641 tatcaaaaga aatatcgcca ccttcataag gtaatgagaa gccaaatttg ccttcttctt
    11701 gttgctgact gtgtaattga gctacgagtt gccctaattg gcgttgactc cctgaagccc
    11761 cttcttctaa atacgtcatc actaaatacg catcaccgtt aacctcgcca cttgcaatta
    11821 ctctaggtgc cgtgatacct gcacgttcaa atttatttaa acctgcaatt tctgcagcat
    11881 aaaatgattc tttacgtcca cgttggacaa gtaagaaaaa tgtatccgta tccgtttcga
    11941 ctcgatatgc ttcgtttaca tcgccaccac tcactcgtga aatctcttta atatctttta
    12001 aaggtaaatg ctctaaccat tgttcattca tacaaacacc ttccattacg cttactttat
    12061 actattatat tgcctaatta ggatgattta atctcaaaat aacttgtggt acttgcgtca
    12121 attttcaaaa ctttttagtt ttaactagga aattatgtag ggaaatgcta gtctattaaa
    12181 agaatatatt tcacatatat taagttgagg atagtaagcc tcaaatataa tgaaatagga
    12241 gcatactcat gtacaaatta attaaacctt tcttattcaa aatcgaaccc gaaaaagcac
    12301 acggactaac tatcgatgca ttaaaaacgt tacaaaaatt cccagtttta ttcccagtcg
    12361 tcgataaact atttacttat aagaatccaa cgttatcaca aacgatacaa ggtaatacgt
    12421 acgacaatcc aattggctta gcagctggtt tcgacaaatc ttgcgaagta ccaaaagcat
    12481 tggaacacct tggattcggt gctttagaat taggtggtat cacgcctaaa cctcaaccgg
    12541 gtaaccctca accacgcatg tttagattat tagaagatga tgccttgata aatcgaatgg
    12601 gcttcaataa tattggtatg aacaaagcac taagtcattt gcgtaaaaat gcttatcaag
    12661 tacctgttgg tatcaatgtt ggtgtgaata aaatgacacc ttatgaagcg cgttatcaag
    12721 attatataaa agtcattgat acgtttaaac acgacgtttc atttttcaca gtcaatatca
    12781 gttctccaaa tactgaaaac cttcaaaatt tccatgataa agatgaattt tcaatgttat
    12841 gtcaagcttt gacaacattt aaaaaacagc acgatgtaac agtgccaatt tacttaaaac
    12901 taacgtctga tatggatttc gatggcttaa aagcactatt accagcgatt actgagacat
    12961 ttgacggtat catcttagca aacacaacgc gacaacgaga tggtttaact tctgctaata
    13021 aagtcgaaga aggcggtttg agtggtcgtc cattatttga acgtaattta aaattgatta
    13081 agtatgctta tcagcaaaca aatggtgaat ttttaattat aggcacaggc ggcgtattca
    13141 gtattgaaga tgcaatcaaa atgatgcgtc acggtgcgtc acttattcaa atttattcat
    13201 cacttgttat tgaaggccca ggtttaacta agaaaatgaa caaaggcatc gcacgttact
    13261 taaaagatca tcattttgac aatgtcagtg atattatagg actagatgcc taatccaacg
    13321 tagtcaaata atactttaaa acttgctgaa tcgtcttatt aaaattatga tataactcaa
    13381 gaagatatat ttatcaccat ttttcaaaat aatggcttaa atatcacata tcattaaata
    13441 tagcgtttta taacggaggc gtttgttatg actaaagaaa cagataataa aaaaacagta
    13501 gcgacttctg acaacaaaga cacacagcaa caaaatcaac ctgaagaaga tgaacgtaaa
    13561 gggttgaatg gttatcgtaa aacagatctt gatttagaaa tcgagcaaga gctacgtgaa
    13621 atgatgaaaa caggagaaaa tgaaacgaaa aatgactaca aaaagtttaa agtgttttca
    13681 cttatttcaa cacttgtcat tgtcatttta gcaattatac gatttgttca taaaatgatg
    13741 taattagagt gagacattgt tttatgcctc aggatccagt tattcattat agctacaata
    13801 tttacgatga tataaataac ccgagatttt agtatgattt atttcactaa agtctcgggt
    13861 ttctatttga taatttttaa tagggcatgg cttgtatatg cgcctgcctt ttatctcatt
    13921 tccaatgatt aatctggata ttgttctaaa aatgctttcg cttctttatt aactgtttta
    13981 aaatcaatac cttgttgcat cgctgcaaag acacatccac aataacactg cctaaagata
    14041 ttatagtcat tacacatttc tatggaacgc tcataacctt tacttttctt aaaatcactt
    14101 ggcaaatagt tcacatcgta tattttttgg acatccatac caagttcatt gattaattgt
    14161 gcgttctttt taggtgataa cgtgattgca ctgccaaaat aatcatagcc atgttctaca
    14221 gctgctttcg ctacaatatc caaacgcatt tcaaaacaag cggtacaacg taagccgcct
    14281 tctttttcat cagctaattc tttatccttc accattttca caaacttatg cggttcataa
    14341 ggtgcttcaa tatacttcac attcgcacca gtcttgcgat taaaatcttc cacaaattgt
    14401 tcttgcactt tagcacgtcg taaatactca tttttcggat gaatatttga attcgcgaaa
    14461 taaattgcaa tgtctgcata ttttgttaaa aactctaatg tatatgtact acaaggtgca
    14521 caacaactat gcaataagat tttaggtctg attgcttctc tttcccactg cccgattaat
    14581 ttcttcaaca ccttgtcata attaattttt tgatttttca ttttactgat aataggttca
    14641 gcattaatca tgattcaacc tcccacattt aatcattaac ttctattata tatgattcat
    14701 attaaatgtc agtcaaaaaa gttagaaatt cattttaatg catttatatt tcgaaaatcc
    14761 cttatgtatc taaaagcatt ttttaaactt gaattttaaa acactaaaca acacatacgt
    14821 ctctgtgtca ttttcatttt tttgttatat atatgtttac tttatttaaa tcaatttcac
    14881 cttataattt atcatgtatt ttacaaaaga ttgacttcaa ttcatcgtaa aagttatact
    14941 tttgccattt tttaatgtaa catggtgtta gtaataaaaa taatacattg aggtgtttta
    15001 catgacagca ttattccctt atattgcttt tgaaaattca aaagaagccc ttgcatatta
    15061 cgaagaagta tttggtgcaa ctgacgttaa acgtttagaa gttggcgaag aacaagcgtc
    15121 acattttggt atgactaagg aagaagcgca agaagcaact atgcatgctg aatttgaagt
    15181 gcttggcgta aaagtgctat gttctgattc ttttggtcgc gctgacaaaa ttaataatgg
    15241 catatcatta ttaattgatt atgatgttaa caataaagaa gatgctgata aagttgaagc
    15301 attctatgag caaattaaag atcattcttc aattgaaata gaattaccgt ttgctgacca
    15361 attctggggt ggcaaaatgg gcatctttac cgataaatac ggtgttcgtt ggatgttaca
    15421 tggtcaagac tatactgcaa tccagcaata atgttattga taaagatata atttaataat
    15481 gtgttctaac gttatttaga aaggcaagct actctcgatt catcgcttgc cttattttta
    15541 ttgtatattt ttaattatcg ttgcaggccc ttgattgaca atcgtattaa aatgccttat
    15601 taagtcaact ttgtctatac ggtttggatc ttctacccaa tgtcttatca aagacaatcc
    15661 cgcacctgaa acataactca tgaaatagga aaatggtata ccattaattt gatcattttc
    15721 atttttataa cgttgtaaat gacacataat taaatcataa agtttttcgt ggatatttga
    15781 atctttaccc atacgaaaca tcaattgata aaatacgatg tctttttcta tcatttctat
    15841 taaaacggtc ataatttgat gtatgttatc cgtggataac ttaactgctc catttaactt
    15901 ctcatcatga atgaagtttc ttatttcctc caactgctgg tcctctaatt tttcaagcaa
    15961 atcatattta tcataataat acgtataaaa tgtactacgg ttaacatcag ctaaatctgc
    16021 aatttgttgc acagtaatct cttctaattg gtgttgatgt aaaagttcaa taaatgcatt
    16081 tctcattgca acttgtgatt ttctaatacg tcgatctata gtcatttgta tcaagtcctc
    16141 cccaatgatt ataaacaatt ataaacgtta tgttcattat cctacaaatc tccaacattg
    16201 atgattggca cacaatgttt acctgtttaa tataggtgat acaaacaaac agaaaaaggt
    16261 gataacaatg aaccaacatt tactaggaaa tccaaaatta actgtcactc atgtcaatga
    16321 agtgaaagcc ggtattaacc acatcgttgt cgacagtgtt caatatggaa atcaagaaat
    16381 gattatggaa aaagatgtca ctgtggaaat gcgcgatggc gaaaaattat atattaatat
    16441 tttcagacca aataaagatg gcaaattccc tgtagttatg tctgcagata cttacggtaa
    16501 agataataag cctaaaatca caaatatggg tccactttgg ccaacattag gcaccattcc
    16561 gacatctagt tttacgcctg aagaatcacc agacccagga ttttgggtgc caaatgatta
    16621 tgttgtagtt aaagttgcat tacgcggtag tgacaaatca aaaggcgtct tatctccatg
    16681 gtcaaaaaga gaagcggaag attattacga agtgattgaa tgggcagcaa atcagtcatg
    16741 gagtaatgga aatatcggga caaatggcgt ttcttatctt gcggtgactc aatggtgggt
    16801 cgcatcatta aatccaccac atttaaaagc aatgattcct tgggaaggct taaatgatat
    16861 gtatagagaa gtatcctttc acggaggtat accggatact ggcttttatc gtttctggac
    16921 tcaaggtatt tttgcgagat ggacagataa tccaaatatc gaagatttga ttcaagcaca
    16981 acaagaacac cctttgttcg atgatttttg gaaacagcgt caagtgccat tatcagaaat
    17041 taaaacacct ctactaacat gtgctagttg gtctacacaa ggtttgcaca accgtggctc
    17101 ttttgaagga tttaaacaag ctgcatcaga agaaaaatgg ttatatgtgc atggacgtaa
    17161 agagtgggaa agttactacg ctagagaaaa tctcgaacgc caaaaatcat tctttgattt
    17221 ttaccttaaa gaagaaaata acgattggaa agatacgcct catgtcattt atgaagttag
    17281 agatcaattt tataaaggcg aattcaagtc agcgtcagcg ttccctttac ctaacacaga
    17341 atatacacca ttgtatttga atgctgaaaa tcacacattg aatcatgcaa agattagtag
    17401 cgcgcatgtc gcacaatatg actctttgga taaacaacaa gatattcgtt ttaaatatac
    17461 gtttgacaaa gatactgagt tagttggaaa catgaactta aaactatggg tacgcactac
    17521 agactcagac gatatggatt tatttgcagg tattaaaaag ttagatcgtc gtggtaatga
    17581 agttaacttc cctgatttta atcatattga aaatggtcaa gttgccactg gttggttgcg
    17641 tgtatcacat cgtgaattag atcaagaaaa atctacaatt gcgcaacctt ggcataaaca
    17701 tgaaacagaa ttaaagttgt cacaagatga gattgtacct gttgaaatcg aattgttacc
    17761 ttcaggaacg ttatttaaac aaggtgaaac attggaagtt attgtaaaag gtagtgaaat
    17821 tgtaattggt aatagtaccc caggcatgaa aacgcgttat gagcacgaag aaactgtaaa
    17881 taaaggcatg cacatgattt atactggtgg taaatatgat tcacaattaa tcattcctgt
    17941 cattaattga tatgcagcga ttacggtcta ttttgattaa aagtgacata gtgaaaacat
    18001 tgtataacaa gagaagacca cacgcttggc atcttaaacc aaggtgtggc ccttttttat
    18061 tattgatggc tatttaattt taaaaatctt gtcataaagc ttactacaag caatgttaga
    18121 ccagtaaata aagccattat tgctgtatta ctttcgttgt tttctcctgt ttttggcaat
    18181 tctttcaatt gttgcttatg tcctgacttg ctcttgctat cccaatgatt actttgttgt
    18241 ttagctattt gtttattact ttttgtgtga gtatcatttg ctttaacact tggatttaca
    18301 gcaccattat tcgatgttac attgccgttt ttcaactgat taataacatt tggatctttc
    18361 gtaccatcta ttaaattatc tggatgtttt agtaaatgtc tgcagtccga tactatttta
    18421 gaaccattgg cctttaccgc attattttgc acctcattat catctttcaa ctgcttgata
    18481 acatctggat ctttcgtgcc atctattaaa ttatcaagtt ttttcacacg tgctatgtca
    18541 ctaactggcc caggataatt tgcatccggt tgtctaactg gtattaaatt gctgttttca
    18601 ttcacacctt caggcttgtt attttcggca ttcgcattat ccgtaaaaat caacactgat
    18661 aaaattgcaa caaataaact tataactttt ttcatcatat ccaccttctt cagaccatca
    18721 ttctatttca tataatttta ttatataatt gttatcaatt caatattttt cactttcctg
    18781 aaaataaata acatgtcatt accttatctc gctaagttaa acattctatt ttctaccaaa
    18841 taaattctgc atactgtttt agaaaaacta cttcttgtta aatatgtcca tcatttaacg
    18901 ctgataaaat tgcaacgata atacctttac ttctattacg ctaatccaat aagcataaaa
    18961 ataaaaaccc ctaatggata accacattgt tatctactag aggtttctat cttatttaat
    19021 tttataacac tatcgtattt tctttttcat gaatcatttc aataatgaca ttgtcttcat
    19081 tcattactgc tactttaggt gcatggtgtt taatttcttc ttcattcaac tgtgcataag
    19141 tcatgattat gactacatcg cctacttcaa caagtcttga cgctgcaccg tttaaacaaa
    19201 ttttaccact acctctttta ccagctatta cgtatgtttc aaaacgtgca ccattattat
    19261 tattcacgat ggctactttt tcatttggca agatgtctac cgcttccaat atatctgaat
    19321 caatcgtaat gctacctaca taatttaaat ttgactcagt cactcttgct ctatgaattt
    19381 tagcattcat cattgttctt atcactttat tcagctccaa ttattatatt atctattaaa
    19441 cgcgcttttg aaaatttaac agctaacgag ataaaaatgc gtccagttat ttcgtgttgt
    19501 tctactaatt gaggataact ataaacagca acttcttcaa tgcgtccact tatatgtgat
    19561 tcaagatatt gagtaaccct atctataatt actttacttt gacgttcacc gtcttgatac
    19621 aacgcttgtg ctaattgcaa acttttactt aaatgtaccg cttcttgtcg ttctttctcc
    19681 gttaaataaa catttctcga acttttcgcc aaaccatctg cttctcgaac gatatcaata
    19741 ccaataattt caacggcatg attaaagtct tttaccattt gctcgacaat agccaattgc
    19801 tgggcatctt ttttaccaaa ataagcataa tccggcataa caatattaaa tagcttatta
    19861 actactgtta ccaccccatc aaaatgccct ggacgcttcg ctccttctaa cacatcagct
    19921 aatggaccta ctttgacatc aatacctaat tcacctggat acatatcttc tactgcagga
    19981 tgaaaaacta tgtccgcccc tacttctgat actaattcta aatctttatc aatttgtctc
    20041 ggataagcat cgaaatcttc gtttggacca aattgtaatg gattaacaaa tacactcaca
    20101 actgtaatat catttgtact aactgattcg cgtaccatcg ttaaatgtcc atcatgtaag
    20161 gcacccattg ttgggataaa accaatcgtt gtgcctgagc gtttggctgc tttaacaatg
    20221 tgttgcatct cttttaccgt agtaatcagc ttagtcattg ttattaacct cattcataat
    20281 tttcttttta tacgtatatt cttctgatgg aaatgcacca gatttaactt cttgatcgta
    20341 ttgttttaaa ccatccacac caacactaaa atcagcaaat tgcttcacaa atttcgcttt
    20401 atgttcaaca ccataattta acatatcgtg ataaaccaat acttgaccat ctgtaccttt
    20461 tcctgcacca ataccaatga ctggaattgt taagtgcttg ctaatttctt ctgctaaatc
    20521 atttggaatt gcttcaagta ctaacgcaac tgcaccagct tgttctacat ttttcgcatc
    20581 taaaataagt tgctccgctg cttctttcgt tgcaccttgt aatttatacc ccataacgcc
    20641 aacactttga ggtgttaatc ctaaatgtgc aactacagga ataccaattg ccgttgcttt
    20701 ttcaataaat ggtgtaatat gcgctccttc tgctttaatt gcatttgcat tcgtctcctg
    20761 ataaagcttt agagcatgat ttaagtcttg tgtcatagag atgcctactg caccaatcgg
    20821 catatcaaca actacaaatg tatttggtgc gcctcttctt actgcacgac cgtgatgaat
    20881 catatctgct aacgtcactt gtacggtact ttcataacct aatacagtca taccaagtga
    20941 atccccaaca agaatcatat caatacccgc tgcttccact tgtttagcac ttggaaaatc
    21001 ataagctgtt accatagaaa ttttagtttg cttttgtttc atatctatta attgacttac
    21061 tgttttcaat gttattcaac ctctttttgc agtattatta gaaatagtat aatgaatctt
    21121 ttatagaaag gaaagaagaa gatgttatca gttgcgatta tcggcccagg cgctgtcggt
    21181 acaactattg cctatgaatt acaacaatca ttgcctcata caacgcttat cgggagacac
    21241 gccaaaacaa taacatatta tactgtacca catgcacctg cacaagatat tgttgtgaaa
    21301 ggttatgaag atgtcacaaa cacatttgat gttattatta ttgcagtcaa aacacatcaa
    21361 cttgatgcag ttattcctca tttaactcat cttgcacatg aggacacgct catcatttta
    21421 gcccaaaatg gttatggtca actcgaacat attccattta aaaacgtatg ccaagcagtt
    21481 gtctatataa gtggtcaaaa gaaaggcgat gttgttacgc acttcagaga ttatcaatta
    21541 cgtatacaag ataatgcatt aactcgacaa tttagagatt tggtccaaga cagtcaaatc
    21601 gacattgtat tagaagcaaa tattcagcaa gctatttggt ataaattgtt agttaacttg
    21661 ggcattaatt ctatcacagc actcggtaga cagacagttg caatcatgca taatcctgaa
    21721 atacgtacac tgtgtagaca gctattacta gatggttgtc gtgttgcaca ggcagagggg
    21781 ttaaactttt cagaacaaac tgtcgatact attatgacta tatatcaagg ttatcccgac
    21841 gaaatgggaa cgagcatgta ttacgacatt gtacatcagc aacctcttga agtcgaagcg
    21901 attcaaggct ttatttatca acgtgcacgt gaacacaatc ttgacacccc atatcttgat
    21961 actatttata gctttttacg cgcgtatcaa caaaatatgt aagcacatat ataatataaa
    22021 aagaccttaa cgatgcattt agaaccacct gttcctaata tcgctaaggt cttttaatta
    22081 tattagacta ttcagcttct ctaatttctt ctgccacatc tttatagtct attttcgctt
    22141 taacaaacgt ctcgttattt accgggaaat gttgttggaa tgtttcaaag ttttgtatct
    22201 caacgacaac gtcatccact tcaaagtcaa gaacatgtcc accataagct ctttcatcat
    22261 ccgcaaaatg tatatgaaaa ccagcagacc ctacgccatg aaataattct ggtgtaaaaa
    22321 atccaacaat ggcaccacga atatcttgtc ttttttcctc aggttgtctg cgtgctgaat
    22381 caatcaaacg tgtatatggc ggttgctgag caggcatcat tcgtacatgc atatgtttaa
    22441 atgtgccata aattttaaca gcggaaaata aattctcact taacatttca tttttaattt
    22501 gtgcgaatac atcatcttgt gataattgtt gcaatggaaa tgtcttactc gctttaaaat
    22561 tagtaatcga tgcatacggt actttctcat cgccttttaa ttctataaat tctttatgct
    22621 cgttagcatg atatgccttc ccgtctaaaa atattacttc gccatcagac cctgttaacg
    22681 ttgcaatccc taaattccca tgttctaaca attcattaat tgtagctgtt ccttctagta
    22741 agccagccat taacgtacct aatgtaccat gttgatacaa gacattcgtc attccaaaac
    22801 catctcccta tagttattcc tcgttgtcat gctctacttc gtgcttaatg cagaactact
    22861 ttcctttata ctcaatgacg cctcgacatt tctattcatg tcactaaatt ttcacgagtc
    22921 ataatacttt attcaattat tattttacgc aaaagtattg cctctatcaa tgcttgctaa
    22981 aaaaatggac ctatcagtgc cacattttac tgcaccacta atagatcctc ttaccattaa
    23041 tatatttcta attaatactt aaaataggct tagtcttcta ataaatattt aattgaatca
    23101 aatgtatctt ctaatgtttt aacagattta tcgaacaatg cttgttcttc tgcacttaat
    23161 ggcatttcat aaattttaac tgcgccatgt tggttaacta atgttggtac acctaggtaa
    23221 acgcctttgt gaccaccata ttgaccatct aattgtatag aaacatttaa gacattattt
    23281 tcattattta aaatggcttt tgaaatgcgc atcaatgcta atgcaatacc atagtatgtt
    23341 gaccctttag cttggataat ttcataagca gcgtcacgtg tatttacata aatttcttcc
    23401 gctttagctt cgctaccagt ttgttctttt aatgtgtcat atactgaaat acctgctaca
    23461 tttgcttgtg accaaactgc aagttcagta tcaccatgct caccaataat actagcgtca
    23521 acacttgaag gtgcaacacc aagttcttgg ctaattaaat attgtaaacg tgcactgtcc
    23581 aatacagtac ctgaaccgat aacacgctct gctggcaatc cagtatattc ttttacaaat
    23641 cttgttaaaa tgtctacagg gtttgccgcg attaagaaat atccatcaaa gccgctatcc
    23701 attacactct taacaatgct cttcataatc ttagtatttt tttcaactaa ttgtaaacgt
    23761 gtttcacctg gcttttgagg tgcaccagct gtaataacaa ctaaatctgc atctttacag
    23821 tcttcgtatt cacctgcttt cacatcaact ggtgaaggac tgtggactgt accatggttt
    23881 aaatcttgaa catctgcttt tactttgtct tttgcaatgt caataattac aaattcatca
    23941 gcaacacctt gcgtaaccat tgcaaaggca tagcttgatc ctacagatcc atctccgatt
    24001 aatacaacct ttttaccaaa tgttttcata attatgtctc ctaacctaat tgaatttatt
    24061 ttacacgtga ctacacgtaa aaatatctat actgaaatta aagattaaca attttatttt
    24121 attataatat tatgctacca aattacaaac ataaaactga gatgtaagcg gagtcaaatt
    24181 acactctaac gccaataaat attgatatct caagctttag cacgtaattt gaaatttgtg
    24241 acaattttta gcattctaaa attgcgtaat gacgctattt aaaggcttta acaaactatt
    24301 gagctttcta tttttttcat tttaaaaagg atggctattc gacttaaatg acttactata
    24361 taacctccta ttttccgcct taccctcatc atatttgatg catcatttta tagtcaaaga
    24421 attacatttc atacaaacta gccaatcaga cataaacatg aaattgattt taaaaatata
    24481 atgtgcaaaa ttgttagaaa at
//
LOCUS       DAHHRR010000029        24211 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.029, whole genome shotgun sequence.
ACCESSION   DAHHRR010000029 DAHHRR010000000
VERSION     DAHHRR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24211
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.029"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..1532
                     /locus_tag="NBI42_001002"
     CDS             <1..1532
                     /locus_tag="NBI42_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCD1777434.1"
                     /translation="DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTE
                     FAIFMGRGGGKNGLISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDN
                     KRNKTGKTPKAPYEVSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGP
                     EMVNVKRGGLGKKKNRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCK
                     LDDPKEVDDRQTWEKANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMN
                     LPEVDLEKVIAPWKEILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIW
                     LGHSFVRQGFLDDVKLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKV
                     IADNYRTDIVRRAFEDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWF
                     TNNVAVKVKPDGNKEYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSID
                     F"
     gene            1548..2735
                     /locus_tag="NBI42_001003"
     CDS             1548..2735
                     /locus_tag="NBI42_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD1777435.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            2719..3456
                     /locus_tag="NBI42_001004"
     CDS             2719..3456
                     /locus_tag="NBI42_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCD1777436.1"
                     /translation="MKVEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            3480..4625
                     /locus_tag="NBI42_001005"
     CDS             3480..4625
                     /locus_tag="NBI42_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCD1777437.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETTFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELMQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            4645..4929
                     /locus_tag="NBI42_001006"
     CDS             4645..4929
                     /locus_tag="NBI42_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777438.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            4919..5203
                     /locus_tag="NBI42_001007"
     CDS             4919..5203
                     /locus_tag="NBI42_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCD1777439.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            5187..5549
                     /locus_tag="NBI42_001008"
     CDS             5187..5549
                     /locus_tag="NBI42_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCD1777440.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            5546..5950
                     /locus_tag="NBI42_001009"
     CDS             5546..5950
                     /locus_tag="NBI42_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCD1777441.1"
                     /translation="MSVKVIGDKALERELEKRFGIKEMVKVQDKALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVYWRGSKDRYKIVHLIEYGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            5947..6354
                     /locus_tag="NBI42_001010"
     CDS             5947..6354
                     /locus_tag="NBI42_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777442.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDECAYSYIVQIDVFVKYNDEYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSSRVYEGIFYKEEN"
     gene            6355..6999
                     /locus_tag="NBI42_001011"
     CDS             6355..6999
                     /locus_tag="NBI42_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1777443.1"
                     /translation="MAVKHASAPKAYINITGLGFAKLTKEGAELKYSDITKTKGLQKI
                     GVETGGELKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEDYDEDGVYEEK
                     QGKQNNYVAVWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLVDNKKSVRKYIFDSANMTNHDGDGEKGEEAFLKKILGEEYTGNVTEDSEETL"
     gene            7041..7265
                     /locus_tag="NBI42_001012"
     CDS             7041..7265
                     /locus_tag="NBI42_001012"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCD1777444.1"
                     /translation="MPDSIKTLKVGDTYDLNVVVEPSNQSKLLKYTTDQTNIVSINSD
                     GQVTAEAQGIATVKATVGNMSDTITINVEA"
     gene            7315..7665
                     /locus_tag="NBI42_001013"
     CDS             7315..7665
                     /locus_tag="NBI42_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777445.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFTVKDLKERMHAPDGMNALREQVIFITQG
                     QQTEETRNFIQNMK"
     gene            7704..7853
                     /locus_tag="NBI42_001014"
     CDS             7704..7853
                     /locus_tag="NBI42_001014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777446.1"
                     /translation="MDTLMMDLIENGKDANEVLKMPFHYVLSIYQNKNNDISEEKAEA
                     LIDAF"
     gene            7910..12421
                     /locus_tag="NBI42_001015"
     CDS             7910..12421
                     /locus_tag="NBI42_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCD1777447.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTHSYKQRIKELDGTITGYKKNVDDLAKQYGKVSQEQGENSAEAQKLRQEYNK
                     QANELNFLEKELEKTTTEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGLMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGGELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELESATESFLKFSHITGSEGVQAVQLITRAMG
                     DAGIEADEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWTSIESAFAPVMEELIKKLSIAVDWFSNLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLAGIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNGAIESVKQTFSNFIQFIQPFIDSVKNIFKQAISAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKAIFEFIINFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGALNIILGFIKFFSSLFTGNWRGVWDGIVMILKGTVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLISGIWGVIKGIFTKSLSAIWNATKSIFGFLYNSVKS
                     IFTNMKNWLSSTWNNIKSNTVGKAHSLFTGVRSKFTSLWNATKDIFTKLRNWMSNIWN
                     SIKDNMVGIAGRLWDKVRNIFGSMRDGLKSIIGKIKDHIGGMVDAVKRGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEMI
                     EFPNGKRVLTPNTDTTAYLPKGSKVYNGAQTYSMLNGTLPRFSIGTMWKDIKSGASSA
                     FNWTKDQIGKGTKWLGDKVGDVMDFIDNPGKLLNYVLQAFGVDFSSLTKGMGIAGDIT
                     KAAWSKIKKSAIKWLEDAFAESGDGGVLDMNKLRYLYGHTAAYTRETGRPFHEGLDFD
                     YIYEPVPSTINGRAQVMPFHNGGYGKWVKIVKGALEVIYAHLSKYKVKTGQQVRVGQT
                     VGISGNTGFSTGPHLHYEMRWNGRHRDPLPWLRKNNGGGKSTPGGNGAANARRAIKAA
                     QNILGGRYKASWITNEMMRVASRESNYTANAVNNWDSNARAGIPSRGMFQMIDPSFRA
                     YAKSGYNNPLNPTHQAISAMRYIVGKWVPRTGSWRAAFKRAGDYAYATGGKVFDGWYN
                     LGEDGHPEWIIPTDPARRNDAMKILHYAAAEVRGKKASKNKRPSQLSDLNGFDDPSLL
                     LKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQESTKVK
                     FRKGGIAIQ"
     gene            12418..13902
                     /locus_tag="NBI42_001016"
     CDS             12418..13902
                     /locus_tag="NBI42_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCD1777448.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGIKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFTIPVKFTIKVVLTDPYKYSVTGNKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFPDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            13918..17703
                     /locus_tag="NBI42_001017"
     CDS             13918..17703
                     /locus_tag="NBI42_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777449.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            17693..17845
                     /locus_tag="NBI42_001018"
     CDS             17693..17845
                     /locus_tag="NBI42_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777450.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            17892..18179
                     /locus_tag="NBI42_001019"
     CDS             17892..18179
                     /locus_tag="NBI42_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777451.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            18237..18533
                     /locus_tag="NBI42_001020"
     CDS             18237..18533
                     /locus_tag="NBI42_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCD1777452.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            18683..18859
                     /locus_tag="NBI42_001021"
     CDS             18683..18859
                     /locus_tag="NBI42_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCD1777453.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(18912..19019)
                     /locus_tag="NBI42_001022"
     CDS             complement(18912..19019)
                     /locus_tag="NBI42_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777454.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            19071..19325
                     /locus_tag="NBI42_001023"
     CDS             19071..19325
                     /locus_tag="NBI42_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCD1777455.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            19337..20092
                     /locus_tag="NBI42_001024"
     CDS             19337..20092
                     /locus_tag="NBI42_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1777456.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
     gene            20283..20774
                     /gene="sak"
                     /locus_tag="NBI42_001025"
     CDS             20283..20774
                     /gene="sak"
                     /locus_tag="NBI42_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HCD1777457.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
     gene            21424..21759
                     /locus_tag="NBI42_001026"
                     /pseudo
     CDS             21424..21759
                     /locus_tag="NBI42_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(21854..22303)
                     /locus_tag="NBI42_001027"
     CDS             complement(21854..22303)
                     /locus_tag="NBI42_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HCD1777458.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
     gene            22988..23338
                     /gene="scn"
                     /locus_tag="NBI42_001028"
     CDS             22988..23338
                     /gene="scn"
                     /locus_tag="NBI42_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HCD1777459.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
     gene            complement(23391..23651)
                     /locus_tag="NBI42_001029"
     CDS             complement(23391..23651)
                     /locus_tag="NBI42_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777460.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
     gene            complement(23962..24141)
                     /locus_tag="NBI42_001030"
     CDS             complement(23962..24141)
                     /locus_tag="NBI42_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777461.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
BASE COUNT         8889 a         3312 c         4811 g         7199 t
ORIGIN      
        1 ttgatgaaca gaaaatcgag gattgtatca aatttattga aaaatggtat tttccaacat
       61 taccatttca aaggtttatc atagctaata tatttcttat agataaaaat acagatgaag
      121 ctttctttac agaatttgct attttcatgg gacgtggagg cgggaaaaac ggtctaataa
      181 gtgctattag tgattttctt tctacgccct tacacggagt taaagaatat cacatctcca
      241 ttgttgctaa tagtgaagat caagcaaaaa catcgtttga tgaaatcaga accgttttaa
      301 tggataacaa acgaaataag acgggtaaaa cgccaaaagc tccttatgaa gttagtaaag
      361 caaaaataat aaaccgtgca actaaatcgg ttattcgata taacacatca aacacaaaaa
      421 ccaaagacgg tggacgtgag gggtgtgtta tttttgatga aattcattat ttctttggtc
      481 ctgaaatggt aaacgtcaaa cgtggtggat taggtaaaaa gaaaaataga agaacgtttt
      541 atataagtac tgatggtttt gttagagagg gttatatcga tgcaatgaag cacaaaattg
      601 caagtgtatt aagtggcaag gttaaaaata gtagattgtt tgctttttat tgtaagttag
      661 acgatccaaa agaagttgat gacagacaga cgtgggaaaa ggcgaaccca atgttacata
      721 aaccgttatc agaatacgct aaaacactgc taagcacgat tgaagaagaa tataacgatt
      781 taccattcaa ccgttcaaat aagcctgaat tcatgactaa gcgaatgaat ttgcctgaag
      841 ttgaccttga aaaagtaata gcaccatgga aagaaatact agcgactaat agagagatac
      901 caaatttaga taatcaaatg tgtattggtg gtttagactt tgcaaatatc cgagatttcg
      961 caagtgtagg gctactattt cgaaagaacg acgattatat ttggttagga cattcttttg
     1021 taagacaagg gtttttggat gatgtcaaat tagagccacc tattaaagaa tgggaaaaaa
     1081 tgggattatt gaccattgta gatgatgatg tcattgaaat tgaatatata gttgattggt
     1141 ttttaaaggc tagagaaaaa tatgggcttg aaaaagtcat agctgataat tatagaactg
     1201 atattgtaag acgtgcgttt gaggatgctg gcataaaact tgaagtactt agaaatccaa
     1261 aagcaataca tggattactt gcaccacgta tcgatacaat gtttgcgaaa cataacgtaa
     1321 tatatggaga caatcctttg atgcgttggt ttactaataa tgttgcagta aaggttaaac
     1381 ccgatggtaa taaagaatat attaaaaaag atgaaaatag aagaaaaacc gatgggttca
     1441 tggcttttgt tcacgcatta tatagagcag acgatatagt agacaaagac atgtctaaag
     1501 cgcttgatgc attaatgagt atagatttct aatagaggag gtgagacatg agtattctag
     1561 aaaagatatt taaaactagg aaagatataa catatatgct tgatttagat atgatagaag
     1621 atctatcaca acaagcgtat gtgaaacgtt tagcgattga tagttgtatt gaatttgttg
     1681 cgcgagctgt cgctcaaagt cattttaaag tattggaagg taatagaatt caaaagaatg
     1741 atgtttacta caagttaaat ataaaaccaa atactgactt atcaagcgat agtttttggc
     1801 aacaagttat atataaacta atttatgata acgaggtttt aatcgtagta agtgacagca
     1861 aagaattact tatcgcagat agcttttaca gagaagagta cgctttgtat gatgatatat
     1921 tcaaagatgt aacggttaaa gattatactt atcaacgtac tttcacaatg caagaggtca
     1981 tatatttaaa gtacaacaac aataaagtga cacactttgt agaaagtcta ttcgaagatt
     2041 acgggaaaat attcggaaga atgataggtg cacaattaaa aaactatcaa ataagaggga
     2101 ttttgaaatc tgcctctagc gcatatgacg aaaagaatat agaaaaatta caagcgttca
     2161 caaataaatt attcaatact tttaataaaa atcaactagc aatcgcgcct ttgatagaag
     2221 gttttgatta tgaggaatta tctaatggtg gtaagaatag taacatgcct ttttctgaat
     2281 tgagtgagct aatgagagat gcaataaaaa atgttgcgtt gatgattggt atacctccag
     2341 gtttgattta cggagaaaca gctgatttgg aaaagaacac gcttgtattt gagaagttct
     2401 gtttaacacc tttattaaaa aagattcaga acgaattaaa cgcgaaactc ataacacaaa
     2461 gcatgtattt gaaagataca agaatagaaa ttgtcggtgt gaataaaaaa gacccacttc
     2521 aatatgctga agcaattgac aaacttgtaa gttctggttc atttacaagg aatgaggtgc
     2581 ggattatgtt aggtgaagaa ccatcagaca atcctgaatt agacgaatac ctgattacta
     2641 aaaactacga aaaagctaac agtggtgaaa atgatgaaaa agaaaaagat gaaaacactt
     2701 tgaaaggtgg tgatgaagat gaaagtggag attaaaggcg tcatcgtttc caacgaagat
     2761 aaatgggttt acgaaatgct tggtatggat tcgacttgtc ctaaagatgt tttaacacaa
     2821 ctagaattta gtgatgaaga tgttgatatt ataattaact caaatggtgg taacctagta
     2881 gctggtagtg aaatatatac acatttaaga gctcataaag gcaaagtgaa tgttcgtatc
     2941 acagcaatag cagcaagtgc ggcatcgctt atcgcaatgg ctggtgacca catcgaaatg
     3001 agtccggttg ctagaatgat gattcacaat ccttcaagta ttgcgcaagg agaagcgaaa
     3061 gatctaaatc atgctgcaga aacattagaa catgttggtc aaataatggc tgaggcatat
     3121 gcggttagag ctggtaaaaa caaacaagaa cttgtagaaa tgatggctag ggaaacgtgg
     3181 ctaaatgctg atgaagccat tgaacaaggt tttgcggata gtaaaatgtt tgaaaacgac
     3241 aatatgcaaa ttgtagcaag caatacacaa gtgttatcga aagatgtatt aaatcgtgta
     3301 acagctttgg taagtaaaac gccagaggtt aacattgata ttgacgcaat agcaaataaa
     3361 gtaattgaaa aaataaatat gaaagaaaag gaatcagaaa tcgatgttgc agatagtaaa
     3421 gtatcagcaa atggattttc aagattcctt ttttaataca aaaaatagga ggtcataaaa
     3481 tgactataaa tttatcggaa acattcgcaa atgcgaaaaa cgaatttatt aatgcagtaa
     3541 acaacggtga accgcaagaa agacaaaatg aattgtacgg tgacatgatt aaccaactat
     3601 ttgaagaaac taaattacaa gcaaaagcag aagctgaaag agtttctagt ttacctaaat
     3661 cagcacaatc tttgagtgca aaccaaagaa gtttcttcat ggatatcaat aaaaacgtta
     3721 actataaaga agaaaaactt ttgccagaag aaacaattga tagaattttt gaagatttga
     3781 cgacgaatca tccgttatta gctgatttag gtattaaaaa cgctggtttg cgtttgaagt
     3841 tcttaaaatc tgaaacttct ggcgtagccg tttggggtaa aatctatggt gaaattaaag
     3901 gtcaattaga tgctgcgttc agtgaagaaa cagcaattca aaataaattg acagcgtttg
     3961 ttgttttacc aaaagattta aatgattttg gtcctgcgtg gattgaaaga tttgttcgtg
     4021 ttcaaatcga agaagcattt gcagtggcgc ttgaaactac gttcttaaaa ggtactggta
     4081 aagaccaacc aatcggctta aaccgtcaag tacaaaaagg tgtatcggta actgagggtg
     4141 cttatccaga gaaagaagaa caaggtacgc ttacatttgc taatccgcgc gctacggtta
     4201 atgaattgat gcaagtgttt aaataccact caactaacga gaaaggtaaa tcagtagcgg
     4261 ttaaaggtaa tgtaacaatg gttgttaatc cgtccgatgc ttttgaggtt caagcacagt
     4321 atacacattt aaatgcaaat ggcgtatatg ttactgcttt accatttaat ttgaatgtta
     4381 tcgagtctac agtccaagaa gcaggtaagg ttttaacgta cgttaaaggt ttatatgatg
     4441 gttatttagc tggtggtatt aatgttcaga aatttaaaga aacacttgcg ttagatgata
     4501 tggatttata cactgcaaaa caatttgctt acggcaaagc gaaagataat aaagttgctg
     4561 ctgtttggaa attagattta aaaggacata agccagcttt agaaggtacc gaagaaacac
     4621 tataaaattt tatgaggtga taaaatggtg aaatttaaag ttgttagagc ttttaaagac
     4681 atagagcaca atcaacacaa gtacaaagta ggggagttgt atccagctga agggtataac
     4741 aatcctcgtg ttgaattgtt gacaaatcaa atcaaaaata agtacgacaa agtttatatc
     4801 gtacctttag ataagctgac aaaacaagaa ttattagaac tatgcgaatc attacaaaaa
     4861 aaagcgtcta gttcaatggt taaaagtgaa atcgtcgact tattgaatgg tgaagacaat
     4921 gacgattgat gatttgcttg tcaaatttaa atcacttgaa aagattgacc ataattcaga
     4981 ggatgagtac ttaaagcagt tgttaaaaat gtcgtacgag cgtataaaaa atcagtgcgg
     5041 agtttttgaa ttagagaatt taataggtca agaattgata cttatacgcg ctagatatgc
     5101 ttatcaagat ttattagaac acttcaacga taattacaga cctgaaataa tagatttttc
     5161 gttatctcta atggaggtat cagaagatga agaaagtgtt taaaaaacct agaattacaa
     5221 ctaaacgttt aaatactcgt gttcattttt ataagtatac tgaaaataat ggtccagaag
     5281 ctggagaaaa agaagaaaaa ttattatata gctgttgggc gagtattgat ggtgtctggt
     5341 tacgtgaatt agaacaagct atctcaaacg gaacccaaaa tgacattaaa ttgtatattc
     5401 gtgatccgca aggtgattat ttacccagtg aagaacatta tcttgaaatt gaatcaagat
     5461 atttcaaaaa tcgtttgaat ataaagcaag tatcaccaga tttggataat aaagacttta
     5521 ttatgattcg tggaggatat agttcatgag tgtgaaagtg ataggtgata aagcattaga
     5581 aagagaatta gaaaaacgtt ttggcataaa agagatggta aaagttcaag ataaggcgct
     5641 aatagctggt gctaaggtaa ttgttgaaga agtaaaaaaa caactaaagc cctcaaaaga
     5701 tacgggagca ttaattaatg aggtaagttt tagtaaacct gaatggataa acggaaaacg
     5761 tacaattact gtttattggc gaggttctaa agaccgttat aaaatcgtac atttaattga
     5821 atatggacac gttcaaaaag gaacaggtaa atttatcaaa cctaaagcta tgggcggtgt
     5881 taatagagca ataagacaag ggcaaaataa gtattttgag acgctaaaaa gggagttgaa
     5941 aaaattgtga ttgatatttt gtacaaagtt catgaagtga ttagtcaaga cagaattatt
     6001 agagagcacg taaatatcaa taatattaag ttcaataaat accctaatgt aaaagatact
     6061 gatgtacctt ttattgttat tgacgatatc gacgacccaa tacctacaac ttatactgac
     6121 ggagatgagt gtgcatatag ttatattgtc caaatagatg tttttgttaa gtacaatgat
     6181 gaatataatg cgagaatcat aagaaataag atatctaatc gcattcaaaa gttattatgg
     6241 tctgaactaa aaatgggaaa tgtttcaaat ggaaaaccgg aatatataga agaatttaaa
     6301 acatatagaa gctctcgcgt ttacgagggc attttttata aggaggaaaa ttaaatggca
     6361 gtaaaacatg caagtgcgcc aaaggcgtat attaacatta ctggtttagg tttcgctaaa
     6421 ttaacgaaag aaggtgcgga attaaaatat agtgatatta caaaaacaaa aggattacaa
     6481 aaaattggtg ttgaaactgg tggagaactt aaaacagctt atgctgatgg cggtccaatt
     6541 gaatcaggga atacagacgg agaaggtaaa atctcattac aaatgcatgc gttccctaaa
     6601 gagattcgca aaattgtttt taatgaagat tatgatgaag atggcgttta cgaagagaaa
     6661 caaggtaaac aaaacaatta cgtagctgta tggttcagac aagagcgtag agacggtaca
     6721 tttagaacag ttttattacc taaagttatg tttacaaatc ctaaaatcga tggagaaacg
     6781 gctgagaaag attgggattt ctcaagtgaa gaggttgaag gtgaggcact tttcccttta
     6841 gttgataata aaaagtcagt acgtaagtat atctttgatt cagctaacat gacaaatcat
     6901 gatggagacg gtgaaaaagg cgaagaggct ttcttaaaga aaattttagg cgaagaatat
     6961 actggaaacg tgacagagga tagcgaagaa actttgtaac gaaaccggct tcatcggaaa
     7021 ctgcggtaaa gtcggttaat ataccagata gcattaaaac acttaaagtt ggcgacacat
     7081 acgatttaaa tgttgtagta gagccatcta atcaaagtaa gttattgaaa tacacaacag
     7141 atcaaacgaa tattgtatca atcaatagtg atggtcaagt tactgcggaa gcacaaggca
     7201 ttgctacggt taaagcaaca gttggtaata tgagtgacac tataacaata aatgtagaag
     7261 cataagaggg ggcaacccct ctattttatt tgaaaataag gagagtatta taaaatggca
     7321 aaattaaaac gtaacattat tcaattagta gaagacccga aagcaaatga aattaaatta
     7381 caaacgtact taacaccaca cttcatttca tttgaaattg tatacgaagc aatggattta
     7441 atcgatgata ttgaggacga aaatagcacg atgaaaccaa gagaaatcgc tgacagattg
     7501 atggatatgg ttgtaaaaat ttacgataac caattcacag ttaaagacct aaaagaacgt
     7561 atgcatgcac ctgatggaat gaatgcactt cgtgaacaag taattttcat tactcaaggt
     7621 cagcaaactg aggaaactag aaattttatc cagaacatga aataaagcct gaagatttaa
     7681 catataaagc aatgttgaaa aatatggata ctctcatgat ggacttaatt gaaaatggta
     7741 aagacgctaa cgaagtttta aaaatgccat ttcattatgt actttccata tatcaaaata
     7801 aaaacaatga catttctgaa gaaaaagcag aggctttaat tgatgcgttt taaccttaac
     7861 cgtttggtta gggttatttt tttgaacttt tttagaaagg aggtaaaaaa tgggagaaag
     7921 aataaaaggt ttatctatag gtttggattt agatgcagca aatttaaata gatcatttgc
     7981 agaaatcaaa cgaaacttta aaactttaaa ttctgactta aagttaaccg gtaacaactt
     8041 caaatatacc gaaaaatcaa ctcatagtta caaacaaagg attaaagaac ttgatggaac
     8101 tatcacaggt tataagaaaa acgttgatga tttagccaag caatatggca aggtatctca
     8161 agaacagggc gaaaacagcg cggaagctca aaaattacga caagaatata acaaacaagc
     8221 aaatgagctg aattttttag aaaaagaact agaaaaaaca acaactgagt ttgaagagtt
     8281 caaaaaagct caagttgaag ctcaaagaat ggcagaaagt ggctggggaa aaaccagtaa
     8341 agtttttgaa agtatgggac ctaaattaac aaaaatgggt gatggtttaa aatccattgg
     8401 taaaggtttg atgattggtg ttaccgcacc tgttttaggt attgcagcag catcaggaaa
     8461 agcttttgca gaagttgata aaggtttaga tacagttacc caagcaacag gagcaaccgg
     8521 cggagagctt aagaagttgc agaattcatt taaagatgtt tatggcaact ttccagcaga
     8581 tgctgagact gtaggcggtg ttttagggga agttaacaca aggttaggtt tcactggcaa
     8641 agaacttgag agtgccacag agtcattctt gaaatttagt cacataacag gttctgaagg
     8701 cgtacaagcc gttcaattaa ttacgcgtgc aatgggtgat gcaggtattg aagctgatga
     8761 gtatcaaagt gtacttgata tggtagcgaa agcagcacag gctagcggta taagtgttga
     8821 tacattagct gatagcatta ctaaatacgg tgctccaatg agggctatgg gctttgagat
     8881 gaaagaatca atcgctttat tctctcaatg ggagaaatca ggtgttaata ctgaaatagc
     8941 cttcagtggt ttgaaaaaag ctatatccaa ttggggtaaa gctggtaaaa atccaagaga
     9001 agaatttaag aagacattag cagaaattga aaagacgccg gatatagcta gcgcaacaag
     9061 tttagcgatt gaagcatttg gtgcaaaagc aggtcctgat ttagcagatg ctattaaagg
     9121 tggtcgtttt agttatcaag aatttttaaa aactatcgaa gattcccaag gcacagtaaa
     9181 tcaaacgttt aaagattctg aaagtggctc cgaaagattt aaagtagcaa tgaataaatt
     9241 aaaattagta ggtgctgatg tatggacttc tattgaaagt gcgtttgcac cagtaatgga
     9301 agaattaatc aaaaagctat ctatagcggt tgattggttt tccaatttaa gtgatggttc
     9361 taaaagatca attgttattt tcggtggtat tgctgctgca attggtcctg tagtttttgg
     9421 attaggcgca tttataagta caattggcaa tgcagtaact gtattagccc cactattagc
     9481 tggtattgca aaggctgatg gattaattag ttttttatcg actaaagtac ctatattagg
     9541 aactgtcttc acggctttaa ctggtccaat tggcattgta ttaggtgttt tggctggctt
     9601 agcagtcgca tttacaattg cttataagaa atctgaaact ttcagaaatt ttgttaatgg
     9661 tgcaattgaa agtgttaaac aaacatttag taattttatt caatttattc aacctttcat
     9721 tgattctgtt aaaaacatct ttaaacaagc gatatcagca atagttgatt ttgctaaaga
     9781 tatttggagt caaattaatg gattctttaa tgaaaacgga atttctattg ttcaagcgct
     9841 tcaaaatata tgcaatttta tcaaagctat atttgaattt attataaatt ttgtaattaa
     9901 accaatcatg tttgcgattt ggcaagtgat gcaatttatt tggccggcgg ttaaagcttt
     9961 gattgtcagc acttgggaaa atatcaaagg tgtaatacaa ggtgctttaa atataatact
    10021 tggctttatt aagttctttt caagtttatt cactggtaat tggcgaggtg tttgggacgg
    10081 tattgtgatg atactaaaag gcactgtgca gttaatttgg aatttaatac aactgtggtt
    10141 tgtaggtaag attctaggtg ttgttagata ctttggtgga ttgcttaaag gtttaatatc
    10201 cggtatctgg ggtgttatca aaggtatttt cacaaaatca ttatctgcaa tttggaatgc
    10261 aacgaaaagt atttttggtt tcttatacaa tagtgttaaa tctattttca ctaatatgaa
    10321 aaactggtta tctagtacgt ggaataatat caaaagcaat accgtcggca aggctcattc
    10381 gttatttacg ggtgtaaggt ctaaattcac aagtttatgg aatgcgacga aagatatatt
    10441 tactaaatta agaaattgga tgtcaaacat ctggaactct attaaagata acatggtagg
    10501 tatagctggt cgtttgtggg ataaagtgcg taacatcttt ggaagcatgc gtgacggttt
    10561 aaaatctatc attggtaaaa ttaaagatca tatcggtggt atggtagacg ctgttaaaag
    10621 aggtcttaat aaattaattg aaggtttaaa ctgggtcggt ggtaagttgg gtatggacaa
    10681 aataccgaag ttacacactg gtactgaaca tacgcatact actacaagat tagttaagaa
    10741 cggtaagatt gcgcgggata cgttcgctac ggttggggat aaaggacgtg gaaatggtcc
    10801 gaatggtttc agaaatgaaa tgattgaatt ccctaatggc aaacgggtac ttacgcctaa
    10861 tacagatacg acagcgtact tacctaaagg ttcaaaagta tataacggcg cacaaactta
    10921 ttcaatgtta aatggaacgc ttccaagatt tagcataggt actatgtgga aagatattaa
    10981 atccggtgca tcatcggcat ttaactggac aaaagatcaa ataggtaaag gtacaaagtg
    11041 gcttggcgat aaagttggtg atgtcatgga ctttatcgat aatccaggca aacttttaaa
    11101 ttatgtactt caagcgtttg gagttgattt cagttctcta actaaaggta tgggtattgc
    11161 tggcgatata acaaaagctg catggtctaa gattaagaaa agtgcaatca agtggcttga
    11221 ggatgctttc gcagagtcgg gtgatggcgg tgtattagat atgaataaat tacgttactt
    11281 atacggtcac actgctgctt atacacgaga aaccggacgc ccattccatg aaggtctgga
    11341 ttttgattac atttacgaac ctgttccatc aaccattaat ggtagagcac aagttatgcc
    11401 ttttcataat ggtggttatg gaaaatgggt gaaaattgta aagggcgcct tagaagttat
    11461 ttatgcacat ttatctaaat ataaagttaa aactggtcaa caagttaggg tcggccagac
    11521 tgttggtata tcggggaata cggggtttag tacaggacct cacttacatt atgagatgcg
    11581 ctggaatgga agacatagag acccgttacc gtggttaaga aagaataatg ggggcggcaa
    11641 aagtacaccc ggtggtaatg gtgcagctaa tgctagacga gctattaagg ctgctcaaaa
    11701 tattttagga ggaaggtata aggcgagttg gattactaac gagatgatgc gtgttgcgag
    11761 tcgtgaatcc aattatacag ctaatgcagt caataattgg gatagcaacg caagagctgg
    11821 tataccttca agaggtatgt tccaaatgat agatccttca tttagagcgt acgcaaagtc
    11881 gggttacaat aatcctctca acccaactca tcaagctata tcggctatga gatatattgt
    11941 gggtaaatgg gtaccaagaa caggctcatg gagagctgcg ttcaaacgcg ctggtgatta
    12001 cgcatatgct acaggtggaa aagtttttga tggttggtat aacttaggtg aagacggtca
    12061 tccagaatgg attattccaa cagatccagc tcgtagaaat gatgcaatga agattttgca
    12121 ttatgcagca gcagaagtaa gagggaaaaa agcgagtaaa aataagcgtc ctagccaatt
    12181 atcagactta aacgggtttg atgatcctag cttattattg aaaatgattg aacaacagca
    12241 acaacaaata gctttattac tgaaaatagc acaatctaac gatgtgattg cagataaaga
    12301 ttatcagccg attattgacg aatacgcttt tgataaaaag gtgaacgcgt ctatagaaaa
    12361 gcgagaaagg caagaatcaa caaaagtaaa gtttagaaaa ggaggaattg ctattcaatg
    12421 atagacacta ttaaagtgaa caacaaaaca attccttggt tgtatgtcga aagagggttt
    12481 gaaataccct cttttaatta tgttttaaaa acagaaaatg tagatggacg ttcggggtct
    12541 atatataaag ggcgtaggct tgaatcttat agttttgata tacctttggt ggtacgtaat
    12601 gactatttat ctcacaacgg cattaaaaca catgatgacg tcttgaatga attagtaaag
    12661 ttttttaact acgaggaaca agttaaatta caattcaaat ctaaagattg gtattggaac
    12721 gcttatttcg aaggaccaat aaagctgcac aaagaattta caatacctgt taagttcact
    12781 atcaaagtag tactaacaga cccttacaaa tattcagtaa caggaaataa aaatactgcg
    12841 atttcagacc aagtttcagt tgtaaatagt gggactgctg acactccttt aattgttgaa
    12901 gcccgagcaa ttaaaccatc tagttacttt atgattacta aaaatgatga agattatttt
    12961 atggttggtg atgatgaggt aaccaaagaa gttaaggatt acatgcctcc tgtttatcat
    13021 agtgagtttc gtgatttcaa aggttggact aagatgatta ctgaagatat tccaagtaat
    13081 gacttaggtg gtaaggtcgg cggtgacttt gtgatatcca atcttggcga aggatataaa
    13141 gcaactaatt ttcctgatgc aaaaggttgg gttggtgctg gcacgaaacg agggctccct
    13201 aaagcgatga cagattttca aattacctat aaatgtattg ttgaacaaaa aggtaaaggt
    13261 gccggaagaa cagcacaaca tatttatgat agtgatggta agttacttgc ttctattggt
    13321 tatgaaaata aatatcatga tagaaaaata ggacatattg ttgttacgtt gtataaccaa
    13381 aaaggagacc ccaaaaagat atacgactat cagaataaac cgataatgta taacttggac
    13441 agaatcgttg tttatatgcg gctcagaaga gtaggtaata aattttctat taaaacttgg
    13501 aaatttgatc acattaaaga cccagataga cgtaaaccta ttgatatgga tgagaaagag
    13561 tggatagatg gcggtaagtt ttatcagcgt ccagcttcta tcatagctat ctatagtgcg
    13621 aagtataacg gttataagtg gatggagatg aatggattag gttcattcaa tacggagatt
    13681 ctaccgaaac cgaaaggcgc aagggatgtc attatacaaa aaggtgattt agtgaaaata
    13741 gatatgcaag caaaaagtgt tgtcatcaat gaggaaccaa tgttgagcga gaaatcgttt
    13801 ggaagtaatt atttcaatgt tgattctggg tacagtgaat taatcataca acctgaaaac
    13861 gtctttgata cgacggttaa atggcaagat agatatttat agaaaggaga tgagagtgtg
    13921 atacatgttt tagattttaa cgacaagatt atagatttcc tttctactga tgacccttcc
    13981 ttagttagag cgattcataa acgtaatgtt aatgacaatt cagaaatgct tgaactgctc
    14041 atatcatcag aaagagctga aaagttccgt gaacgacatc gtgttattat aagggattca
    14101 aacaaacaat ggcgtgaatt tattattaac tgggttcaag atacgatgga cggctacaca
    14161 gagatagaat gtatagcgtc ttatcttgct gatataacaa cagctaaacc gtatgcacca
    14221 ggaaaatttg agaaaaagac aacttcagaa gcattgaaag atgtgttgag cgatacaggt
    14281 tgggaagttt ctgaacaaac cgaatacgat ggcttacgta ctacgtcatg gacttcttat
    14341 caaactagat atgaagtttt aaagcaatta tgtacaacct ataaaatggt tttagatttt
    14401 tatattgagc ttagctctaa taccgtcaaa ggtagatatg tagtactcaa aaagaaaaac
    14461 agcttattca aaggtaaaga aattgaatat ggtaaagatt tagtcgggtt aactaggaag
    14521 attgatatgt cagaaatcaa aacagcatta attgctgtgg gacctgaaaa tgacaaaggg
    14581 aagcgtttag agctagttgt gacagatgac gaagcgcaaa gtcaattcaa cctacctatg
    14641 cgctatattt gggggatata tgaaccacaa tcagatgatc aaaatatgaa tgaaacacga
    14701 ttaagttctt tagccaaaac agagttaaat aaacgtaagt cggcagttat gtcatatgag
    14761 attacttcta ctgatttgga agttacgtat ccgcacgaga ttatatcaat tggcgataca
    14821 gtcagagtaa aacatagaga ttttaacccg ccattgtatg tagaggcaga agttattgct
    14881 gaagaatata acataatttc agaaaatagc acatatacat tcggtcaacc taaagagttc
    14941 aaagaatcag aattacgaga agagtttaac aagcgattaa acctaataca ccaaaaatta
    15001 aacgacaata ttagcaatat caatactata gtaaaagatg ttgtagatgg tgaattagaa
    15061 tactttgaac gcaaaattca taaaagtgat acaccgccag aaaatccagt caatgatacg
    15121 ctttggtatg atacaagtaa ccctgatgtt gctgtcttgc gtagatattg gaatggtcga
    15181 tggattgaag caacaccaaa tgatgttgaa aaattaggtg gtataacaag agagaaagcg
    15241 ctattcagtg aattaaacaa tatttttatt aatttatcta tacaacacgc tagtcttttg
    15301 tcagaagcta cagaattact gaatagcgag tacttagtag ataatgattt gaaagcggac
    15361 ttacaagcaa gtttagacgc tgtgattgat gtttataatc aaattaaaaa taatttagaa
    15421 tctatgacac ccgaaactgc aacgattggt cggttggtag atacaaaaac tttatttctt
    15481 gagtatagaa agaaattaca agatgtttat acagatgtag aagatgtcaa aatcgccatt
    15541 tcagatagat ttaaattatt acagtcacaa tacactgatg aaaaatataa agaagcgttg
    15601 gaaataatag caacaaaatt tggtttaacg gtgaatgaag atttgcagtt agtcggagaa
    15661 cctaatgttg ttaaatcagc tattgaagca gctagagaat ccacaaaaga acaattacgt
    15721 gactatgtaa aaacatcgga ctataaaaca gacaaagacg gtattgttga acgtttagat
    15781 actgctgaag ctgagagaac gactttaaaa ggtgaaatca aagataaagt tacgttaaac
    15841 gaatatcgaa acggattgga agaacaaaaa caatatactg atgaccagtt aagtgatttg
    15901 tccaataatc ctgagattaa agcaagtatt gaacaagcaa atcaagaagc gcaagaagct
    15961 ttaaaatcat acattgatgc tcaagataat cttaaagaga aggaatcgca agcgtatgct
    16021 gatggtaaaa tttcggaaga agagcaacgc gctatacaag atgctcaagc taaacttgaa
    16081 gaggcaaaac aaaacgcaga actaaaggct agaaacgctg aaaagaaagc taatgcttat
    16141 acagacaaca aggtcaaaga aagcacagat gcacagagga gaacactgac tcgctatggt
    16201 tctcaaatta tacaaaatgg taaggaaatc aaattaagaa ctactaaaga agagtttaat
    16261 gcaaccaatc gtacactttc aaatatatta aacgagattg tccaaaacgt tacagatgga
    16321 acaacaatca gatatgatga taacggagtg gctcaagctt taaatgtggg gccacgtggt
    16381 attagattaa atgctgataa aattgatatt aacggtaata gagaaataaa ccttcttatc
    16441 caaaatatgc gagataaagt agataaaacc gatattgtca acagccttaa tttatcaaga
    16501 gagggtcttg atatcaatgt taatagaatt ggaattaaag gcggtaacaa taacagatat
    16561 gttcaaatac agaatgattc tattgaacta ggtggtattg tgcaacgaac ttggaaaggc
    16621 aaacgatcaa ccgatgatat attcacacgt cttaaagatg gacatctaag gtttagaaat
    16681 aataccgcag gcggttcact ttatatgtca cattttggta tttcaacata tattgatgga
    16741 gaaggcgaag acggaggttc atccggtact attcaatggt gggataaaac ttacagtgat
    16801 agcggtatga atggcataac aatcaattcc tatggtggtg tcgttgcact aacgtcagat
    16861 aataatcggg ttgttctgga gtcttacgct tcatcgaata tcaaaagcaa acaggcaccg
    16921 gtgtatttat atccaaacac agacaaagtg cctggattaa accgatttgc attcacgctg
    16981 tctaatgcag ataatgctta ttcgagtgac ggttatatta tgtttggttc tgatgagaac
    17041 tatgattacg gtgcgggtat caggttttct aaagaaagaa ataaaggtct tgttcaaatt
    17101 gttaatggac gatatgcaac aggtggagat acaacaatcg aagcagggta tggcaaattt
    17161 aatatgctga aacgacgtga tggtaatagg tatattcata tacagagtac agacctactg
    17221 tctgtaggtt cagatgatgc aggagatagg atagcttcta actcaattta tagacgtact
    17281 tattcggccg cagctaattt gcatattact tctgctggca caattgggcg ttcgacatca
    17341 gcgcgtaaat acaagttatc tatcgaaaat caatataacg atagagatga acaactggaa
    17401 cattcaaaag ctattcttaa cttacctatt agaacgtggt ttgataaagc tgagtctgaa
    17461 attttagcta gagagctgag agaagataga aaattatcgg aagacaccta taaacttgat
    17521 agatacgtag gtttgattgc tgaagaggtg gagaatttag gattaaaaga gtttgtcacg
    17581 tatgatgaca aaggagaaat tgaaggtata gcgtatgatc gtctatggat tcatcttatc
    17641 cctgttatca aagaacaaca actaagaatc aagaaattgg aggagtcaaa gaatgcagga
    17701 taacaaacaa ggattacaag ctaatcctga atatacaatt cattatttat cacaggaaat
    17761 tatgaggtta acacaagaaa acgcgatgtt aaaagcgtat atacaagaaa ataaagaaaa
    17821 tcaacaatgt gctgaggaag agtaatcttt agcactattt ttatacaaag atttaaggag
    17881 gtcatttaat tatggcaaaa gaaattatca acaatacaga aaggtttatt ttagtacaaa
    17941 tcgacaaaga aggtacagaa cgtgtagtat atcaagattt cacaggaagt tttacaactt
    18001 ctgaaatggt taaccatgct caagatttta aatctgaaga aaacgctaag aaaattgcgg
    18061 agacgttaaa tttgttatat caattaacta acaaaaaaca acgtgtgaaa gtagttaaag
    18121 aagtagttga aagatcagat ttatctccag aggtaacagt taacactgaa acagtatgaa
    18181 aagctatgag ttagatactc ataatcttta ttcttttaga aagcgggtgt actgaattgg
    18241 ggtggttcaa aaaacacgaa catgaatggc gcatcagaag gttagaagag aatgataaaa
    18301 caatgctcag cacactcaac gaaattaaat taggtcaaaa aacccaagag caagttaaca
    18361 ttaaattaga taaaacctta gatgctattc aaaaagaaag agaaatagat gaaaagaata
    18421 agaaagaaaa tgataagaac atacgtgata tgaaaatgtg ggtgcttggt ttagttggga
    18481 caatatttgg gtcgctaatt atagcattat tgcgtatgct tatgggcata taagagaggt
    18541 gaataaaatg tttaaactaa tctttggtta tagtttctgg acatgttttt ggttcggtaa
    18601 atgtaaataa gttttagtca gtgcttcggt actgactttt tatttattgt tgtaattatg
    18661 gtaatatgca gaagtgagca agttggatag atggtggcta tctgagtata aggaggtggt
    18721 gcctatggtg gcattactga aatctttaga aaggagacgc ctaatgatta caattagtac
    18781 catgttgcag tttggtttat tccttattgc attgataggt ctagtaatca agcttattga
    18841 attaagcaat aaaaaataac catcgctaac tttggctggt ttcgatggtt aaatggttat
    18901 taatttaatc tttaatctaa aatagccacc gtctttttaa cgggctcatt agggtaacat
    18961 gtttgcgcat gttgcccttt ttctatatat aaattaacac accataatat aaatatcaaa
    19021 tagacggctt attagtcgtc tttttatttt gggtaaaagg agataagaat atgattaatt
    19081 ggaaaattag aatgaaacaa aaatcatttt gggtagcgat attgtcagct atctttttat
    19141 ttgctcaaaa catcgcaaaa gctattgggt atgatatcca agtttataca gagcaattaa
    19201 cagacggttt aaacgctata ttaggatttt tagtattaac tggtgtgatt caagacccga
    19261 ctactaaagg tataggtgat agccaccaag ctttagaata tgaagaacca agaagaaaat
    19321 actaggaggt aaaataatga aaacatacag tgaagcaaga gcaaggttac gttggtatca
    19381 aggtagatat attgattttg acggttggta tggttaccaa tgtgcagatt tagcagttga
    19441 ttacatttat tggttgttag aaattagaat gtggggaaat gcaaaagatg caatcaataa
    19501 cgattttaaa aacatggcaa cagtatatga aaacacacca tcgtttgttc cacaaatagg
    19561 tgatgtggct gtatttacca aaggaatata taaacaatac ggtcatattg gtttagtgtt
    19621 taatggtggt aatacaaacc aatttttaat tttggaacag aactatgacg gtaacgcaaa
    19681 tacgcctgca aagttacgtt gggataatta ttacggctgt actcacttta ttagacctaa
    19741 gtataaaagt gagggcttaa tgaataagat cacaaataaa gttaaaccac ctgctcaaaa
    19801 agcagtcggt aaatctgcaa gtaaaataac agttggaagt aaagcgcctt ataaccttaa
    19861 atggtcaaaa ggtgcttatt ttaatgcgaa aatcgacggc ttaggtgcta cttcagccac
    19921 tagatacggt gataatcgta ctaactatag attcgatgtt ggacaggctg tatacgcgcc
    19981 tggaacatta atatatgtgt ttgaaattat agatggttgg tgtcgcattt attggaacaa
    20041 tcataatgag tggatatggc atgagagatt gattgtgaaa gaagtgtttt aattcttagg
    20101 ttaaaatgtt aaatatttgt taattatttt ttaatgtaat tttagtttct tttaatattt
    20161 tattgatttt taatattttc tcaatataaa atgaagtcgt tgatatttat catcttaaat
    20221 aagggtgtta gctataaaaa gagataaata aaaacaaata tattatattt ggaggaagcg
    20281 ccatgctcaa aagaggttta ttatttttaa ctgttttatt gttattattc tcattttctt
    20341 caattactaa tgaggtaagt gcatcaagtt cattcgacaa aggaaaatat aaaaaaggcg
    20401 atgacgcgag ttattttgaa ccaacaggcc cgtatttgat ggtaaatgtg actggagttg
    20461 atggtaaagg aaatgaattg ctatcccctc attatgtcga gtttcctatt aaacctggga
    20521 ctacacttac aaaagaaaaa attgaatact atgtcgaatg ggcattagat gcgacagcat
    20581 ataaagagtt tagagtagtt gaattagatc caagcgcaaa gatcgaagtc acttattatg
    20641 ataagaataa gaaaaaagaa gaaacgaagt ctttccctat aacagaaaaa ggttttgttg
    20701 tcccagattt atcagagcat attaaaaacc ctggattcaa cttaattaca aaggttatta
    20761 tagaaaagaa ataaaacaaa atagttgttt attatagaaa gcaatgtctt gattgaatat
    20821 gtgtagtgaa aattatcttt catcaaattc tcattcatgc acgaatggtt cttccccacc
    20881 taatcagata ttaggtgact tatggggaga aatcagttag gatgaaaaag tggataatcc
    20941 ttttttaggc aggtacttcg gtacttgcct atttttttat gttataatct ttctagacgt
    21001 attcaaggga cgtcttttta gattgtatgt tatagctagc tttcgggcta gttttttgtt
    21061 atgatgtgtt acacatgcat caactattta catctatcct tgttcaccca agcatgtcac
    21121 tgggtgtttt ttcttatgat agagagcata gttttcatac tactccctcg tagtatatat
    21181 gactttagca ttcccgtata atagtttacg gggtgctttt tatgttataa ttaactgtat
    21241 atagtaggag tgaactatat agcctgttaa gtggcctagt aacctaacac ttatcctgca
    21301 attgatatcc tttttgccct tcactcgata catatatctc aacaacatag aaatattaca
    21361 gtcgctacac cgcatcttaa atggtgtggt tatttttatt ggaagtgtgt atcaggtatc
    21421 agtaatgtta aaacaccagc taaaaatgaa aagaattcac cagtgccagc aggttataca
    21481 ctcgataaaa acaatgtacc gtataaaaaa gagactggtt attacacagt tgccaatgtt
    21541 aaaggtaata acgtgaggga tggctattca actaattcaa gaattacagg tgtattaccc
    21601 aataacgcaa ctatcaaata tgacggcgca tattgcatta atggctatag atggattact
    21661 tatattgcta atagtggaca acgtcgttat atagcgacag gagaggtaga caaggcaggt
    21721 aatagaataa gcagttttgg taagtttagt gcagtttgat aattagatat ataaaggttt
    21781 ggcaagttat gaaatgtctg ccaaaccttt atataaaaaa gaaatatcta ccttttaatt
    21841 tatgtaacta ctattagtat gcatattcat tagtttttcc aggaccatta attacataag
    21901 atgatttaga ctctcctttt ttaaagaagt atgttttata cattttacct agtaactcaa
    21961 cattttttct atcttcagca agtggtgtat tcagatatac tgtatagtaa cctttatttt
    22021 cagttaaaat aaccattttt tcaaattgag cagaattttt tgtgcctttc tttaaataat
    22081 ttctcaaacg ttcatctaat tttcctagcg ttgtaggaag accactattt ttaaatgatt
    22141 ctttataagc tttttctttc tctaacattt tcttatttga ttctatttct tcatttgtag
    22201 gaaacggttc aaaagtaaaa gctttcgctg aatgatggtg tgtactgatt cctgccgtta
    22261 aaaaacttaa tgctaaaact gttgttgcta atttcttttt cataatgatg ttaattctcc
    22321 ttaaattcta tatttaaatt tatagttagt tttgcgaaat tcctaaaatg agtttaatct
    22381 aatcgacgaa atatattaat taacttgaaa ttaataaaag attaattatt tttaactaaa
    22441 aattaaaatt caattagtgt tttgatgaat ttggtctcga taaattgaaa taatctaaaa
    22501 aacgctataa tttttctatt aatagtaatt aatatgtgct atatttatct tagacacagc
    22561 aatgtgttca aattttcatc tattcataag ctagccttcg ggctagtttt tttgtgctat
    22621 atatatttgt tttaattaaa taaaattaga taatgcaata gtagccattt tatgttaata
    22681 ttaccttggg cgttttcaag gagcgccttt cattttttat gtattgctcc ccttcgggct
    22741 agtatattaa atttattttt gcgctttcca aatcaatgta tatgtgttat attgtttatg
    22801 ggaagtaggt aagcatttcg gtgcttacct ttttttgttt ttctataaat acaataaggt
    22861 atgtcaattt gataatttat taattttcat ttaataagaa gatctatata gttaatgaat
    22921 aattaatgta ctttttttta gttagtcatt aaaataaatt agtactaatt actaaggaga
    22981 ataaaaaatg aaaattagaa aatctatact tgcgggaact ttagcaatcg ttttagcatc
    23041 accactagta actaatctag ataaaaatga ggcacaagct agcacaagct tgccaacatc
    23101 gaatgaatat caaaacgaaa agttagctaa tgaattaaaa tcgttattag atgaactaaa
    23161 tgttaatgaa ttagctactg gaagtttaaa cacttattat aagcgaacta taaaaatttc
    23221 aggtcaaaaa gcaatgtatg ctcttaagtc aaaagacttt aagaaaatgt cagaagcaaa
    23281 atatcaactt caaaagattt ataacgaaat tgacgaagca ctaaaaagta aatattaaaa
    23341 aaaccaccct tttacgggtg gttttaattt tctagataat ataaaagtgt tcataaataa
    23401 aacagtatag gcaaacaata aagtattgaa aaaagtaagt ttaatatgaa aattgttaaa
    23461 tgaacgacat cttttgtttt tataaatatc aagaaaataa tcaaactcaa aataaataac
    23521 gtaactgtag tcataggcgt ccatacataa tcagcattag tcattaagaa tggtgcagcc
    23581 attatgaaaa aatttataat gcagatgaaa tagacaatta gactataaat tagataaata
    23641 acaatacaca cccttcataa ataaataatt taaatcctat atattttaac aaaagtaaca
    23701 cacagaagtg tagaaaataa aaaatattgg taaataaaat caataagttt aaccaatatg
    23761 ttgctcgctt cataccgtat attgcaacaa aaattccgat taagaaaaat atagccccta
    23821 tgataaaaca gaaatccgat gctgaattat taaaaaatga ggtgtttaga gttagaaaat
    23881 gagttaatga gttgactata actaataaga tattaattat atttgtatgg ttcttcacat
    23941 gatacctcca agtaaaaaaa tctaattaat aaagtgaatg cttgatgaac aagcagttat
    24001 tccaaacaga atcaataaga aaagtagaat caacatgcta atgccccata aacaaccctt
    24061 ttcactttct ctattattaa tttcttgact tcttttaaag atattattac ttttacattc
    24121 tttagttgtt ttaaatttca cgtttttatt acttcctttt gtctaaaagt ttacaatgaa
    24181 tttttgatta taataatata ttcaaaatag t
//
LOCUS       DAHHRR010000030        23556 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.030, whole genome shotgun sequence.
ACCESSION   DAHHRR010000030 DAHHRR010000000
VERSION     DAHHRR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23556
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.030"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(420..493)
                     /locus_tag="NBI42_001031"
     tRNA            complement(420..493)
                     /locus_tag="NBI42_001031"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(457..459),aa:Arg,seq:tct)
     gene            857..991
                     /locus_tag="NBI42_001032"
     CDS             857..991
                     /locus_tag="NBI42_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCD1777462.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            1048..1182
                     /locus_tag="NBI42_001033"
     CDS             1048..1182
                     /locus_tag="NBI42_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCD1777463.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            1306..2001
                     /locus_tag="NBI42_001034"
     CDS             1306..2001
                     /locus_tag="NBI42_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCD1777464.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKI
                     KYFDQTLETIVELWKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCCFNFRGFDHNPGIIPDY
                     EINSWKQLNEIVR"
     gene            complement(2109..2549)
                     /locus_tag="NBI42_001035"
     CDS             complement(2109..2549)
                     /locus_tag="NBI42_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1777465.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            2744..4357
                     /gene="bshC"
                     /locus_tag="NBI42_001036"
     CDS             2744..4357
                     /gene="bshC"
                     /locus_tag="NBI42_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCD1777466.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSGDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLKAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKYFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFIGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNQDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            4501..4932
                     /gene="mraZ"
                     /locus_tag="NBI42_001037"
     CDS             4501..4932
                     /gene="mraZ"
                     /locus_tag="NBI42_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCD1777467.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            4948..5883
                     /gene="rsmH"
                     /locus_tag="NBI42_001038"
     CDS             4948..5883
                     /gene="rsmH"
                     /locus_tag="NBI42_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCD1777468.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQAAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVVPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            5897..6298
                     /gene="ftsL"
                     /locus_tag="NBI42_001039"
     CDS             5897..6298
                     /gene="ftsL"
                     /locus_tag="NBI42_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCD1777469.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            6279..8513
                     /locus_tag="NBI42_001040"
     CDS             6279..8513
                     /locus_tag="NBI42_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD1777470.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVIDMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            8805..9770
                     /locus_tag="NBI42_001041"
     CDS             8805..9770
                     /locus_tag="NBI42_001041"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCD1777471.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVEQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLVSGLIGLWIGVH"
     gene            9772..11121
                     /locus_tag="NBI42_001042"
     CDS             9772..11121
                     /locus_tag="NBI42_001042"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCD1777472.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIYEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            11137..12456
                     /locus_tag="NBI42_001043"
     CDS             11137..12456
                     /locus_tag="NBI42_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HCD1777473.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQAEFDEEIYLINKDFKKEQSNEENNDSASSHANDNNIDDSTDSNNENEDYR
                     YNPEIDDQNESNGIAVDDEQPQSAPKEQNSDSNDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEEDPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKKDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSTQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            12561..13967
                     /gene="ftsA"
                     /locus_tag="NBI42_001044"
     CDS             12561..13967
                     /gene="ftsA"
                     /locus_tag="NBI42_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCD1777474.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKEESKFKKLMKSLFE"
     gene            14000..15172
                     /gene="ftsZ"
                     /locus_tag="NBI42_001045"
     CDS             14000..15172
                     /gene="ftsZ"
                     /locus_tag="NBI42_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCD1777475.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            15432..16223
                     /gene="pgeF"
                     /locus_tag="NBI42_001046"
     CDS             15432..16223
                     /gene="pgeF"
                     /locus_tag="NBI42_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCD1777476.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LNNYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            16247..16915
                     /locus_tag="NBI42_001047"
     CDS             16247..16915
                     /locus_tag="NBI42_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCD1777477.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            16912..17475
                     /locus_tag="NBI42_001048"
     CDS             16912..17475
                     /locus_tag="NBI42_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCD1777478.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            17487..17777
                     /locus_tag="NBI42_001049"
     CDS             17487..17777
                     /locus_tag="NBI42_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCD1777479.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIFVLVLFQKGLLQIFNWILIQLQ"
     gene            17860..18666
                     /locus_tag="NBI42_001050"
     CDS             17860..18666
                     /locus_tag="NBI42_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCD1777480.1"
                     /translation="MVFYNFFKVGINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPV
                     LTHFLDPRGQYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFDLTLME
                     IDYPEKFVTLKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIK
                     GASVKLYTIPVTDMIQSNENWKIESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVK
                     VNHTIVDSADFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            18690..19319
                     /locus_tag="NBI42_001051"
     CDS             18690..19319
                     /locus_tag="NBI42_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCD1777481.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIARAQAQADQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEAQAAANAQNSETSSSESNSQNDSNDSEATKK"
     gene            19537..22290
                     /gene="ileS"
                     /locus_tag="NBI42_001052"
     CDS             19537..22290
                     /gene="ileS"
                     /locus_tag="NBI42_001052"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCD1777482.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNINGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGDIIMTKETVNHVADLFAEHGSNIWFEREAKELLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTAVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSVPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            22581..23378
                     /locus_tag="NBI42_001053"
     CDS             22581..23378
                     /locus_tag="NBI42_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1777483.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDVLGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNDLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKDYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT         8649 a         3257 c         4482 g         7168 t
ORIGIN      
        1 ccagcctgat tttttaaatg tatttaatca taggatttcg ggtccctgaa aagtccctaa
       61 aattttgaat agtatcaaaa tctataggaa taatataata ataaaaatct ctacgcatgc
      121 aaatggtgag tgttaaaaat cttggtgtag ttttggtcta gttcaggtgc aatatagata
      181 aaatttgagc gcaaagttac ttaattaaaa atatgtaaaa atgtatcaac cgcgtgtcaa
      241 ttatgtgtca aaaaattcta aaacgaataa gcatgcttgt tttaattact gtattttcgg
      301 ggcttacgcg ggggaagttt tggtgacgca tcatactata ttactgaaat tcaaaacaaa
      361 aagagccccg taatcacgga actcttttgt ttggtaatgc gtataaaaat acctataaac
      421 gtcctgggag ggattcgaac ccccgaccga tggcttagaa ggccattgct ctatccagct
      481 gagctaccag gacacgttta acaacacaag aattattata tctaaatgaa cttaaattag
      541 caataccttg taaataaaaa atgtttatat ttttcactat tatagagcta tttatctaaa
      601 aaggttcaat aagacttaaa tacgaattca ggcaacttaa ttgtgttaaa tacagttttg
      661 aatgcctaac tgtatatctt ttctctttaa aatacagtta agtactttat aagatgttgt
      721 gcggataaac aaactaattg catcaaattt attttaaaat aacaacaaca aaacgttaag
      781 cgaataacat ttcggtgatt taaaagctac gcacgttttt gttatcttca aatttaaatt
      841 ttaaggagtg ttttcaatgg aaggtttatt taacgcaatt aaagataccg taactgcagc
      901 aattaataat gacggcgcaa aattaggcac aagcattgtg agcatcgttg aaaatggcgt
      961 aggtttacta ggtaaattat tcggattcta attttgatat gttatgtaat taataagtat
     1021 tattttaaag gtgagggaga gatttaaatg actggactag cagaagcaat cgcaaatact
     1081 gtgcaagctg cacaacaaca tgatagtgtg aaattaggca caagtatcgt agacatcgtt
     1141 gctaacggtg tgggtttact aggcaaatta ttcggattct aatataataa ctaatattct
     1201 ttaaaataaa ctgggggagc atactttaat gttatgcact cagtttattt tatttgcaga
     1261 aatttgggcc tctgttaaga tttagataca tagacaatat aggagatggg gaaattggga
     1321 tataaaaata ttttgataga ctttgatgat acaattgttg atttttatga tgcagaagaa
     1381 tgggcgtttc actatatggc gaatgttttt aatcataaag caacaaagga tgatttttta
     1441 acatttaaaa aaatcaatca ccaacattgg gaagcttttc aacaaaataa attaacgaag
     1501 tctgaagtat tatcagaacg atttgtgaat tactttaaac atcatcaaat ggaagttgat
     1561 gggcatcgtg cagatgtgtt atttagaaat ggattagcag aagctaaaat taaatacttt
     1621 gatcaaacat tagaaacaat tgtcgagtta tggaaaagac atgatctata tattgttact
     1681 aatggtgtaa ccgaaacgca aaagagaagg ttaaatcaga cgccgttgca taaatacatt
     1741 aaaaagatat ttatatctga ggaaacaggt tatcaaaaac ctaatccgga attttttaat
     1801 tatgttttta atgatattgg tgaggatgaa agacagcact cgattatagt tggagattct
     1861 ttaacatctg acattctagg tggaatcaat gcgggtatag ctacttgctg ctttaatttt
     1921 agaggatttg atcataatcc aggaattata cctgattatg aaattaattc atggaaacaa
     1981 ctaaatgaaa ttgtacgtta agacaaataa tttgaaaatg atttacttat attagctaag
     2041 aaagtaattg taaaaaagac actacgaaaa gatgcatttt catgctcgta gtgtcgaata
     2101 ttattatctt atggtttcca caataagaca tcatgtccct ctatattttt agcttccaca
     2161 tctgtaaatc catgatgtaa aaagaaatcc ttagaatcat ttctgccaat ggctttaatt
     2221 ggcatattga aactctttgc aaaatcaatc aattctgaag cgtaccctct gttttgatat
     2281 ttcggtaata cttctaactt ccataataat atataatctt caaaatctgg gaagtagatt
     2341 tcttcgacat cacctttttt tagtaatgcc attctagctc ctaattgatc tccgacaaat
     2401 atgccataaa atggtgaatc tgaacttgca tcaatcattt gaccgtttaa ctcattgacc
     2461 atgtataagt ctttgttgcc aaacgctcta aagttttcga ataattcgtc agttttgtaa
     2521 ttaatttcaa gacgtttgat ttcactcata tttatctacc ccttatttgt ttactactat
     2581 tattatacat taaaccactt gtgtttttca ttgatttgaa aatgtaaaac aatgaatatt
     2641 ataaattttc catgattaac ttgattttca agccgtaaaa ttgtcaatca ccggtataga
     2701 ctttatacta tagtttgtaa tcgcgtactt aaggagaaaa aacatggact gtaaagtagt
     2761 tagtttaaat gaaaaagatc agtttatacc aaaaataaag agcggtgacc ctgtaataac
     2821 aggattattt caatatgatg cagctcaaca aactagtttt gaaaaaagga tgtctaaaga
     2881 aaataatgga agagaagcgg cattagcgaa tgttattcgt gaatatatga gtgatttaaa
     2941 gctttcaagt gaacaagaat taaacataca acatttagct aatggttcaa aagttgtgat
     3001 tggtggacaa caagcagggc ttttcggggg accattgtat acattccata aaatattttc
     3061 aatcattact ttatctaagg aattaacgga tacacataag caacaagtag taccagtttt
     3121 ttggattgca ggagaagatc atgatttcga tgaagtgaat catacatttg tttataacga
     3181 aaatcatggg tcactgcata aggttaaata tcatacaatg gagatgccag agactactgt
     3241 ctctagatat tatcctgata aggctgagtt gaaacaaact ttaaaaacga tgttcattca
     3301 tatgaaagaa acggttcata cacaaggtct actggagatt tgtgacagaa ttattgacca
     3361 atatgactcg tggactgata tgtttaaagc actactgcat gagacattta aagcatatgg
     3421 cgttctattt atagatgcgc agtttgagcc gttaagaaaa atggaagcgc ctatgtttaa
     3481 aaagattttg aaaaaacatc agttgcttga tgatgctttt agagcaacac aacaacgtac
     3541 tcaaaatcaa ggcttgaagg cgatgataca aacagataca aatgttcatt tattcttaca
     3601 tgatgaaaat atgcgtcaat tagtttcgta tgatggtaag tattttaaat taaataaaac
     3661 agataagaca tatataaagg aagaaattat aaatattgcg gaaaatcaac ctgaattatt
     3721 ttctaataat gtagtgacaa gaccattaat ggaagaatgg ctatttaaca cggtggcatt
     3781 tattggagga ccgagtgaaa ttaagtactg ggctgaacta aaagatgtat ttgaactatt
     3841 tgatgttgaa atgcctatcg tgatgccaag gcttagaatt acttatttaa atgaccgtat
     3901 tgaaaaatta ctttcgaaat acaatattcc attagaaaaa gtgttagtcg atggtgttga
     3961 aggagaaaga agtaagttta ttagagaaca agcatcacat caatttattg aaaaggtaga
     4021 aggtatgatt gaacaacagc gtcgtctaaa ccaagactta ttagatgaag tggcggggaa
     4081 tcaaaataat attaaccttg tgaataaaaa taatgaaatt catatacaac agtatgatta
     4141 tttgttaaaa cgttatcttt taaacattga aagagaaaac gacatcagta tgaagcaatt
     4201 tagagaaatt caagaaacac tccatccaat gggaggatta caagaaagaa tatggaatcc
     4261 acttcaaatt ttgaatgatt ttgggacaga tgtgttcaag ccctccacct atccaccact
     4321 ttcttacact tttgatcata ttattataaa accttaatat accaagggtt tagcccgatt
     4381 tatcttaatg ataaatcggg catttttttg ttttttaaaa taaatttcac aaattttgta
     4441 taaatagtgg tggatagtgg ggagatgtgg taaattatat ataaggtgag gtgataaaaa
     4501 atgttcatgg gagaatacga tcatcaatta gatacaaaag gacgtatgat tataccgtcc
     4561 aagtttcgtt atgacttaaa tgagcgtttt attatcacta gaggccttga taaatgttta
     4621 ttcggttaca ctctagacga atggcaacag attgaagaga aaatgaaaac cttacctatg
     4681 acaaaaaaag acgcacgtaa gtttatgcgt atgttcttct ctggtgctgt tgaagtagaa
     4741 cttgataagc aagggcgtat taacatccct caaaacttga ggaaatacgc taatttaact
     4801 aaagaatgta cagtaatcgg tgtttcaaat cgtattgaga tttgggatag agaaacttgg
     4861 aatgatttct atgaagaatc tgaagaaagt ttcgaagata ttgctgaaga tttaatagat
     4921 tttgattttt aaaatggagg aattgaagtg tttcatcata tcagcgttat gttaaacgaa
     4981 accattgatt atttaaatgt aaaagaaaat ggtgtgtaca ttgactgtac gctaggtgga
     5041 gcgggacatg ccctttattt actaaatcaa ttaaatgacg acggaagatt aatagcgatc
     5101 gatcaagacc aagctgcaat tgataatgct aaagaggtat taaaggatca tttgcataag
     5161 gtgacttttg ttcatagcaa cttccgtgaa ttaactcaaa tattaaaaga cttaaacatt
     5221 gaaaaagtag atggaattta ttacgacttg ggtgtttcaa gcccacaact cgacattcca
     5281 gaacgaggat tcagttatca ccatgacgca acattagaca tgcgtatgga ccaaacacaa
     5341 gaactaacag catatgaaat tgttaacaat tggtcatatg aagcgttagt gaagattttt
     5401 tatcgctatg gcgaggagaa attttcaaaa cagatagctc gaagaatcga agcacatcgc
     5461 gaacaacaac caataacaac aacattagaa ttagttgaca ttataaaaga aggtattcct
     5521 gcaaaagcaa gaagaaaagg cggacatcct gcaaaacgag tatttcaagc actacgaatt
     5581 gcagtaaacg atgaattgtc agcttttgaa gattcaatag aacaagcgat tgaattagtg
     5641 aaagtagacg gcaggatttc ggtaatcact ttccattctt tagaagatcg tttatgtaaa
     5701 caggtgttcc aagaatatga aaaaggtcca gaggtaccaa gaggattacc agttgtacca
     5761 gaagcatata cacctaagtt aaagcgtgtt aatcgtaaac cgattaccgc tacagaagaa
     5821 gatttagatg acaataacag agcacgaagc gcgaaattac gtgtagctga aatacttaaa
     5881 taaggagcaa tttataatgg ctgtagaaaa agtgtaccaa ccatatgacg aacaagttta
     5941 taatagtata ccgaagcaac aaccacaaac taagcccgaa aagaagactg tttcgagaaa
     6001 agtggttgta caattaacta aatttgaaaa agttttatac ataactttga ttactgtaat
     6061 tgctatgtta agtatttata tgctatcttt aaaaatggat gcgtatgata cgcgaggaaa
     6121 gattgcagat ttagattata aaatagataa acaatcaagt gaaaacagtg ctttacaatc
     6181 tgaaatcaaa aagaattctt cttatgaacg catatacgaa aaggctaaga aacaggggat
     6241 gagccttgag aacgataatg taaaggtagt gcgtagtaat ggcgaagcaa aaaattaaaa
     6301 ttaaaaaaaa taaaataggg gcagtcctac ttgttggttt attcggactg ctctttttta
     6361 tattggtttt aagaatttca tatatcatga ttactgggca ttctaatggt caagatttag
     6421 tcatgaaggc aaatgaaaag tatttagtta agaatgcaca acaaccagaa cgaggaaaga
     6481 tatatgatcg taatggtaaa gtgctagcag aagatgtaga aagatataaa cttgttgcag
     6541 taatagataa aaaggcgagt gccaattcta aaaaacctag gcatgtagtt gataaaaaag
     6601 agactgcaaa gaaattatct acagtcattg atatgaagcc agaggaaatt gaaaagagac
     6661 ttagtcaaaa gaaagccttc caaattgaat ttggacgcaa aggaacaaat ttaacgtatc
     6721 aggacaaatt gaaaatagag aaaatgaatt tgcctggtat ttctttattg cctgaaacag
     6781 aacgctttta tccaaatggc aattttgcat cacacttaat tggtagagct cagaaaaatc
     6841 cagatactgg tgaacttaaa ggtgcgcttg gagttgaaaa gatttttgat agttatttaa
     6901 gtggatctaa aggatcattg agatatattc atgatatttg gggatatatt gcaccaaata
     6961 ctaaaaaaga gaagcagcct aaacgtggtg atgatgtcca tttaacaatc gattcaaata
     7021 ttcaagtatt tgttgaagaa gctttagatg gcatggttga aagataccag ccgaaagatt
     7081 tatttgcggt tgtcatggat gccaaaactg gagaaatttt agcatacagt cagcgaccaa
     7141 catttaatcc tgaaactgga aaagactttg gtaaaaagtg ggcaaatgac ctatatcaaa
     7201 acacatacga gcctggatca acatttaaat catatgggtt agcagctgct attcaagaag
     7261 gtgcttttga tcctgataag aaatataaat ctggacatag agatattatg ggttcacgta
     7321 tttcagactg gaatagagtc ggttggggtg aaatcccaat gtcactcgga tttacttatt
     7381 catctaatac attgatgatg catttacaag atttagttgg tgcagacaaa atgaaatctt
     7441 ggtatgaacg atttggattt ggaaaatcaa ctaaaggtat gtttgatgga gaagcacctg
     7501 gccaaattgg atggagtaat gagttgcaac aaaaaacgtc atcatttggt caatcgacaa
     7561 cagtaacacc tgttcaaatg ttacaagcgc aatcagcgtt ctttaatgat ggtaatatgt
     7621 taaaaccatg gtttgtgaat agcgttgaaa atcctgttag taaaagacaa ttttataaag
     7681 gtcaaaaaca aatcgcaggc aaaccaataa caaaagatac tgctgaaaaa gttgaaaagc
     7741 aattggattt agttgtgaat agtaagaaga gtcacgctgc aaactatcgt attgatggtt
     7801 atgaggtcga aggtaagact ggtacagcac aagtcgctgc acctaatggt ggtggatacg
     7861 ttaaaggtcc aaacccatat ttcgtaagtt ttatgggtga cgcaccgaag aaaaatccta
     7921 aagttattgt atacgctggt atgagcttgg cacaaaaaaa tgaccaagaa gcttatgaat
     7981 taggtgttag taaagcgttt aaaccaataa tggaaaatac tttgaaatat ttaaatgtag
     8041 gtaaatcaaa agatgacaca tctaatgcag agtatagtaa agtgccagat gttgaaggtc
     8101 aagataaaca aaaagctatt gataatgtga gtgcaaaatc attagaacca gttactatcg
     8161 gttctggcac acaaataaaa gcacaatcta taaaagcagg gaataaagtc ttacctcata
     8221 gtaaagtact gttattaaca gatggagact taactatgcc tgacatgtca ggatggacaa
     8281 aagaagatgt cattgctttt gaaaacctaa caaatattaa agtaaattta aaaggtagcg
     8341 gttttgtgtc ccaccaatca attagtaagg gacaaaaact tactgaaaaa gataaaatag
     8401 acgtagaatt ttcatcagag aatgtagaca gcaattcgac gaataattct gattcaaatt
     8461 cagatgataa gaagaaatct gacagtaaaa ctgacaagga taagtcggac taacaagaat
     8521 ggctacatta aataagggtg tattgtcact atttgttttt actaaagtaa tgctgtgata
     8581 gaaaacaaca ttttcattta atctttataa ataagtgttg atagagaatt ttaaggtaga
     8641 cgaggtcaga ttaacttgtt tgttataggc aaacttttta tactggcctc ttatgccgta
     8701 aaaaagaaaa attataaatt ttgattacaa ctttgtttat ttaattgaaa aattgataat
     8761 atattagtga ttaaaaaagt ctggattaag aagaggagat agttatgatt tttgtatatg
     8821 cgttattagc gctagtgatt acatttgttt tggtacctgt tttaatacct acattaaaaa
     8881 ggatgaaatt tggtcaaagt attcgagaag aaggcccaca aagccatatg aagaagactg
     8941 gtacaccaac gatgggtgga ctaacatttc tattaagtat tgtgataacg tctttggtgg
     9001 ctattatatt tgtagaacaa gctaatccga tcatactgtt attatttgtg acgattggtt
     9061 ttgggttaat tggttttata gatgattata ttattgttgt taaaaagaat aaccaaggtt
     9121 taacaagtaa acagaagttt ttggcgcaaa ttggtattgc gattattttc tttgttttaa
     9181 gtaatgtgtt ccatttggtg aatttttcta cgagcataca tattccattt acgaatgtag
     9241 caatcccgct atcatttgca tatgttattt ttattgtttt ttggcaagta ggtttttcta
     9301 atgcggtaaa tttaacagat ggtttagatg gattagcaac tggactgtca attatcggat
     9361 ttacaatgta tgccatcatg agctttgtgt taggagaaac ggcaattggc attttctgta
     9421 tcattatgtt gtttgcgctt ttaggatttt taccatataa cattaaccct gctaaagtgt
     9481 ttatgggaga tacaggtagc ttagctttag gtggtatatt tgctacgatt tcaatcatgc
     9541 ttaatcagga attatcatta atttttatag gtttagtgtt cgtaattgaa acattatctg
     9601 ttatgttaca agtcgctagc tttaaattga ctggaaagcg tatatttaaa atgagtccga
     9661 tacatcatca ttttgaattg ataggatgga gcgaatggaa agtagttaca gtattttggg
     9721 ctgttggtct ggtttcaggt ttaatcggtt tatggattgg agtgcattaa gatgcttaat
     9781 tacacagggt tagaaaataa aaatgtatta gttgtcggtt tggcaaaaag tggttatgaa
     9841 gcagctaaat tattaagtaa attaggtgcg aatgtgactg tcaatgatgg gaaagactta
     9901 tcgcaagatg ctcatgcaaa agatttagaa tctatgggca tttctgttgt aagtggaagt
     9961 catccattaa cattgcttga taataatcca ataattgtta aaaatcctgg aataccttat
    10021 acagtatcta ttatttatga agcagtgaaa cgaggtttga aaattttaac agaagttgag
    10081 ttaagttatc taatctctga agcaccaatc atagctgtaa cgggtacaaa tggtaaaacg
    10141 acagttactt ctctaattgg agatatgttt aaaaaaagtc gcttaactgg aagattatcc
    10201 ggcaatattg gttatgttgc atctaaagta gcacaagaag taaaacctac agattattta
    10261 gttacagagt tgtcatcatt ccagttactt ggaatcgaaa agtataaacc acacattgct
    10321 ataattacta acatttattc agcacatcta gattaccatg aaaatttaga aaactatcaa
    10381 aatgctaaaa agcaaatata taaaaatcaa acggaagaag attatttgat ttgtaattat
    10441 catcaaagac aagtgataga gtcggaagaa ttaaaagcta agacattgta tttctcaact
    10501 caacaagaag ttgatggtat ttatattaaa gatggtttta tcgtttataa aggtgttcgc
    10561 attattaaca ctgaagatct agtattgcct ggagaacata atttagaaaa tatattagca
    10621 gctgtgcttg cttgtatttt agctggtgta cctattaaag caattattga tagtttaact
    10681 acattttcag gaatagagca tagattgcaa tatgttggta ctaatagaac taataaatat
    10741 tataatgatt ccaaagcaac aaacacgcta gcaacacagt ttgccttaaa ttcatttaat
    10801 caaccaatca tttggttatg tggtggtttg gatcgaggga atgaatttga cgaactcatt
    10861 ccttatatgg aaaatgttcg cgcgatggtt gtattcggac aaacgaaagc taagtttgct
    10921 aaactaggta atagtcaagg gaaatcggtc attgaagcga acaatgtcga agacgctgtt
    10981 gataaagtac aagatattat agaaccaaat gatgttgtat tattgtcacc tgcttgtgcg
    11041 agttgggatc aatatagtac ttttgaagag cgtggagaga aatttattga aagattccgt
    11101 gcccatttac catcttatta aagggtgtga gtattgatgg atgataaaac gaagaacgat
    11161 caacaagaat caaatgaaga taaagatgaa ttagaattat ttacgaggaa tacatctaag
    11221 aaaagacggc aaagaaaaag atcaaaggct acacattttt ctaatcaaaa taaagatgat
    11281 acatctcaac aagctgagtt tgatgaagaa atttacttga taaataaaga cttcaaaaaa
    11341 gaacaaagca atgaagaaaa taatgattct gcttctagtc atgcgaacga taataatatc
    11401 gatgattcta cagactctaa taatgaaaat gaggattata gatataatcc agaaattgac
    11461 gaccaaaatg aatcgaatgg aattgcagtc gacgacgaac aacctcaatc agctcctaaa
    11521 gaacaaaata gcgactcgaa tgatgaggaa acagtaacga aaaaagaacg aaaaagtaaa
    11581 gtaacacaat taaagccatt aacacttgaa gaaaagcgga agttaagacg taagcgacaa
    11641 aagcgaatcc aatacagtgt tattacaata ttggtattgt tgattgctgt tatattaatt
    11701 tacatgtttt caccacttag taaaattgcg catgtaaata taaatggaaa taatcacgtt
    11761 agtacttcaa agataaacaa agttttaggt gttaaaaatg attcgaggat gtatacgttt
    11821 agtaaaaaaa atgctattaa tgatctcgaa gaggatccat taatcaaaag tgttgagata
    11881 cacaagcaac taccaaacac attaaacgta gatatcacag aaaatgaaat tattgcttta
    11941 gtgaaatata aaggtaaata tttaccttta ttagaaaatg gtaaattgct taaaggttca
    12001 aatgatgtca aaattaatga tgcacctgtc atggatggtt tcaaaggtac aaaaaaagat
    12061 gatatgatta aggcgttatc tgaaatgaca cctgaagtta gacgatatat tgccgaagtg
    12121 acatacgccc caagtaaaaa caaacaaagc agaattgaat tgtttacgac agatggactt
    12181 caagtaatcg gtgatatttc gacgatatct aagaaaatga aatattatcc gcagatgtca
    12241 caatcattat caagagatag ttcgggtaaa ctaaaaacac gaggctatat tgatttatca
    12301 gtcggtgctt catttatccc ataccgtgga aacacgtcta ctcaatcaga aagcgataaa
    12361 aatgtgacta aatcatctca agaggaaaat caagcaaaag aagaattaca aagcgtttta
    12421 aacaaaatta acaaacaatc aagtaagaat aattaaaaaa ttttgatatt gtctatgttt
    12481 atagttcaca agccattcaa cgtattgtaa actaaagata gtgtattttt taatagtaat
    12541 ttgtcaggag gtgcctatct atggaagaac attactacgt aagtattgat attggatcat
    12601 caagcgtaaa aacaatagta ggcgagaaat ttcacaatgg tataaatgtg ataggtacag
    12661 gacaaaccta cacgagcggt ataaaaaatg gtttaattga tgattttgat attgcgcgtc
    12721 aagcaatcaa agacacaatt aaaaaggcat caatcgcttc gggtgttgat attaaagaag
    12781 ttttcctgaa attacctatc attggaacgg aagtttatga tgaatcaaat gaaatcgact
    12841 tttatgagga tacagaaatc aacggttcac atatcgaaaa agtattagaa ggtattagag
    12901 aaaaaaatga tgtgcaagaa acagaagtaa ttaatgtgtt cccgattcgt tttatagtcg
    12961 ataaagaaaa tgaggtttca gaccctaaag aattaattgc cagacattca ttaaaggttg
    13021 aagcaggcgt aattgctatt caaaaatcga ttttaattaa tatgattaaa tgcgttgaag
    13081 catgtggtgt tgatgtatta gatgtttatt ctgatgcata taactacggt tcaatcctaa
    13141 cagctactga aaaagagtta ggtgcatgtg tcattgatat tggtgaagac gttacgcaag
    13201 ttgcttttta tgaacgcggt gaattagtag atgctgattc tatcgaaatg gcagggcgtg
    13261 atattacaga cgatattgca caaggattaa acacttctta tgaaactgct gaaaaagtta
    13321 aacaccaata tggtcatgca ttctatgatt ctgcttcaga tcaagatatc ttcactgttg
    13381 aacaggttga tagtgatgaa acagtacagt atactcaaaa agatttgagt gactttatcg
    13441 aagcgcgtgt agaagaaata ttcttcgaag tatttgatgt tttacaagat ttaggattaa
    13501 caaaagtaaa tggtggattt attgtaactg gtggatctgc aaacttactt ggcgtaaaag
    13561 aattattgtc agatatggta agtgaaaaag ttagaattca cacgccatca caaatgggaa
    13621 ttagaaaacc tgaattttct tcagcaattt ctacaatttc tagtagtatc gcttttgatg
    13681 agttattaga ttatgttaca attaattatc atgataatga agaaactgaa gaagatgtta
    13741 ttgatgtgaa agacaaagat aacgaatcta aattaggcgg atttgattgg tttaaacgta
    13801 aaacaaacaa aaaagatact catgaaaatg aagtagagtc aacagatgaa gaaatttatc
    13861 aatcagaaga taatcatcag gaacataaac agaatcatga acatgttcaa gacaaagata
    13921 aagaagaaag taaattcaaa aaactaatga aatctctatt tgaatgatta ttggccaata
    13981 aaactaggag gaaatttaaa tgttagaatt tgaacaagga tttaatcatt tagcgacttt
    14041 aaaggtcatt ggtgtaggtg gtggcggtaa caacgccgta aaccgaatga ttgaccatgg
    14101 aatgaataat gttgaattta tcgctatcaa cacagacggt caagctttaa acttatctaa
    14161 agctgaatct aaaatccaaa tcggtgaaaa attaacacgt ggtttaggag caggagctaa
    14221 tcctgaaatc ggtaaaaaag ctgcagagga atctcgtgag caaattgaag atgcaatcca
    14281 aggtgcagac atggtatttg ttacttctgg tatgggtggc ggaactggta ctggtgcagc
    14341 accagtcgtt gctaaaattg caaaagaaat gggcgcatta actgttggtg ttgtaactcg
    14401 tccatttagt tttgaaggac gtaaacgtca aactcaagct gctgctggag tagaagctat
    14461 gaaagctgca gtagatacat taatcgttat accaaatgac cgtttattag atatcgttga
    14521 caaatctacg ccaatgatgg aagcatttaa agaagctgac aacgtgttac gccaaggtgt
    14581 acaaggtatc tcagacttaa tcgctgtttc tggtgaggta aacttagact ttgcagacgt
    14641 taagacaatt atgtctaacc aaggttctgc attaatgggt attggtgttt cttctggtga
    14701 aaatagagcg gtagaagctg ctaaaaaagc aatctcttct ccattacttg aaacatctat
    14761 cgttggtgca caaggtgtgc ttatgaatat tactggtggc gagtcattgt cattatttga
    14821 agcacaagag gctgctgata ttgtccaaga tgctgcagat gaagacgtta atatgatttt
    14881 cggtacagtt attaatcctg aattacaaga tgagattgtt gtaacagtta ttgcaactgg
    14941 ttttgatgac aaaccaacat cacatggtcg taaatctggt agcactggat tcggaacaag
    15001 cgtaaatact tctagcaatg caacttctaa agatgaatca ttcacttcaa attcatcaaa
    15061 tgcacaagca actgatagtg taagtgaaag aacacataca actaaagaag atgatattcc
    15121 tagcttcatt agaaatagag aagaaagacg ttcaagaaga acaagacgtt aatcggttaa
    15181 tatatataca caaataattc aacacaaatc atcagataac atatctgatg atttttttac
    15241 taatttttag aacatgtaga aggacattta agtttttcga agtcattaaa agtgtttaag
    15301 tatcgtgtca aaattaagtc aaaaattatt tgcgcaacat tttaacttta aacataaatg
    15361 ttatattata taattattaa ctttgtacag ttagacgaag ataatttaaa tgaaatgatg
    15421 gtgacgatcg agtgaatgat aattttaaaa agcaaccgca tcatttaata tatgaagaat
    15481 tattacaaca aggtattact ctaggtatta caactagagg cgatggttta agtgactatc
    15541 ctaaaaatgc ttttaatatg gcgagatata ttgatgatcg cccatataat attactcaac
    15601 atcaattgca attagctgaa gaaattgcgt ttgatagaaa aaattgggtg tttcccatcc
    15661 aaacacatga aaataaagtc gcttgtataa caaaggatga tataggcaca aatatagaca
    15721 ctttaactga tgcgcttcat ggtattgatg cgatgtacac atatgatagt aatgtcttat
    15781 taacgatgtg ttatgcagac tgtgtaccag tatattttta tagtacaaaa catcatttta
    15841 ttgcattggc gcatgcaggg tggcgtggta cctatactga aattgtaaaa gaagtgctaa
    15901 aacatgtgaa ctttgatttg aaagacttac atgttgttat tggaccatct acatcatcaa
    15961 gttatgaaat taatgatgat attaaaaata aatttgaaac attgccaatt gatagtgcaa
    16021 actatattga aactagagga cgagatcgtc atggtattga tttgaaaaaa gccaatgctg
    16081 cattattaaa taattatggt gttcctaaag aaaatattta tacgacagcg tatgctacat
    16141 ctgaacattt agaattattt ttctcttatc gattagaaaa aggtcaaaca ggacgcatgt
    16201 tagcattcat tggtcaacag taaacaagga ggagatatgc ttgcgtgtga aagataattt
    16261 acaacaaatc tcaacacaaa ttaatgacaa aagtgaaaaa aataattttt caacaaaacc
    16321 aaacgtgatt gcagttacaa aatatgttac aatagagcga gctaaagaag cgtatgaggc
    16381 tggaataaga cattttggtg agaatagatt ggaaggcttt tttcaaaaga aagaagcatt
    16441 accatcagat gcggtgatcc attttatagg atcattacaa tctcgaaaag ttaaggacgt
    16501 tataaacgac gtagattatt tccatgcttt agatcgattg agcttagcca aagaaattaa
    16561 caaacgtgca gaacataaaa ttaaatgttt cttgcaagtg aacgtttcgg gagaagcttc
    16621 taaacatggt attgctttag aagatgttga tcagtttata gatgatctta aaaaatatga
    16681 caaaatcgaa attgtaggtt taatgacgat ggcaccattg acagatgatg aagcatatat
    16741 tagatcgtta tttaaacagt tacgtttgaa aaaagaagaa atacaacgac tcaatttaga
    16801 atatgcgcct tgtgatgaat tatcaatggg aatgagtaat gactatctta ttgcagttga
    16861 agaaggtgca acgtttgtta gaattgggac taaacttgta ggagaagagg agtgagccac
    16921 ttggctttaa aagatttatt tagtggattt tttgtaatag atgatgaaga ggaagtagaa
    16981 gtacctgaca aacaacaaca ggtaaatgaa gcgccagcaa aagagcagtc acaacaaaca
    17041 acaaaacaaa acgcaatcaa atcagtccct caaaaatctg catcaagata tacaacaacg
    17101 tcagaagaaa ggaataaccg tatgtctaat tattcaaaaa ataattcacg taatgttgta
    17161 actatgaaca atgctacacc aaacaatgca tcacaagaaa gttcaaaaat gtgtttattc
    17221 gaaccacgtg ttttttcaga tacacaagat attgctgatg agcttaaaaa ccgccgtgcg
    17281 acacttgtca atttacaacg tattgataaa gtatcagcga aaagaattat tgatttttta
    17341 agcggtactg tttatgcaat cggtggagat atccaacgtg taggtactga tattttctta
    17401 tgtacgcctg ataatgtgga agtagctgga agcattacag accatattga aaatatggaa
    17461 cattcattcg actaaggagt taacatatgg atataaatgt gctagctaca atatttaaat
    17521 ttatcctttt tgttgttgaa atttactatt tcggcatgat tatatatttc tttacatctt
    17581 gggtaccaag tattagagaa actaaggtag gttatttttt agcgaaaata tatgaacctt
    17641 tcttacaacc atttagaaaa gtaattccac ctattggaat tatcgacata tcatcaatcg
    17701 ctgcaatttt cgttttagta ttattccaaa aagggttact ccaaatcttt aattggattt
    17761 taattcaatt acaataaagt aagcgacata acgaggcaag ggtacatgat actttagcct
    17821 cgtttttgat atgtattttt ctgaatataa gggcaataga tggtatttta taattttttt
    17881 aaggtaggga ttaatataga tatttatcaa cactttagac aggaagaata cgaattaatt
    17941 gatcagctaa cggataaatg tgatcaagcg gaacagcatt atgcaccagt attaacgcat
    18001 tttttagatc caagagggca atatatattg gaagtgattt gtggcagtta tgaagattta
    18061 aacgtatctt tttatggcgg acctaatgct gaaagaaaaa gagcaatcat ttcgccgaac
    18121 tattatgaac ctaaagaaag tgactttgat ttaactttaa tggaaataga ttatcctgaa
    18181 aaattcgtca ctttaaaaca tcaacatatt ttagggacat taatgtcttt aggtatcgaa
    18241 cgcgaacaag ttggagatat aattgtgaat gaacgaattc aatttgtttt gacaagtaga
    18301 ttggaatcat ttattatgtt agaattacaa cgtattaaag gcgcatcagt taaactttat
    18361 actattccag taacagatat gatacaatct aatgagaatt ggaaaattga aagtgcaaca
    18421 gttagttctt taaggttaga tgttgttatt aaagaaatga tacgtaaatc acgtacgatt
    18481 gcgaaacaac taatcgaaaa aaaacgtgtt aaagtgaatc acactattgt tgattcagca
    18541 gattttcaat tacaagcaaa tgatttaata tccatccaag gttttggtag agcacacatt
    18601 actgacttag gtggtaaaac taaaaaagat aaaacgcaca ttacctatag aacattattc
    18661 aaatagtaat gatttaagga ggataacaaa tgccttttac accaaatgaa attaagaata
    18721 aagagttttc acgtgtaaag aatggtttag aacctactga agttgctaat tttttggagc
    18781 aactaagcac tgaaattgaa cgtcttaaag aagataaaaa acaacttgaa aaagtaatcg
    18841 aagagagaga tactaatatt aagtcttatc aagacgtgca tcaatctgta agtgatgctt
    18901 tgatacaagc tcaaaaagct ggtgaagaaa ctaagcaagc tgcagagaaa caagctgaag
    18961 caattattgc tagagcacaa gctcaagctg atcaaatggt tggagatgct gttgaaaaag
    19021 cacgtcgctt agctttccaa actgaagata tgaaacgcca atctaaagtg tttagatctc
    19081 gttttcgtat gctagttgaa gcacaattag acttattaaa gaatgaagat tgggattatt
    19141 tattgaatta tgatttagat gcagaacaag tgacacttga aaacatccat catttacatg
    19201 aaaatgattt aaaaccggat gaagcacaag cagcagcaaa tgcacaaaac agtgaaacaa
    19261 gttcttcaga aagtaattca caaaatgatt ctaatgattc agaggcaaca aaaaagtaat
    19321 taattaaaga cagacgcgta atatacattt aacttttcac agcgaattag gtaacggtga
    19381 gagcctagta aaagcatgta tgttatatca ctggcttttt aatatttaaa taatgtaatg
    19441 agagaactct aagttgagtt aataagggtg gtaccgcgag caatcgtccc ttttaattta
    19501 acttagagtt ttttaaattt ttaaggagtg aaaaaaatgg attacaaaga aacgttatta
    19561 atgcctaaaa cagatttccc aatgcgaggt ggtttaccaa acaaggaacc gcaaattcaa
    19621 gaaaaatggg atgcagaaga tcaataccat aaagcgttag aaaaaaataa aggtaacgaa
    19681 acattcattt tacatgatgg cccaccatac gcgaatggta acttacatat gggacatgcc
    19741 ttgaacaaaa ttttaaaaga ctttattgta cgttataaaa ctatgcaagg gttctatgca
    19801 ccatacgtac caggttggga tacacatggt ttaccaattg aacaagcttt aacgaaaaaa
    19861 ggtgttgatc gtaaaaagat gtcaacagct gagttccgtg aaaaatgtaa ggagtttgct
    19921 ttagaacaaa ttgaactaca gaaaaaagac tttagacgtt taggtgttcg tggtgacttt
    19981 aatgatccat acattacatt aaaacctgaa tatgaagctg cacaaattcg tatttttgga
    20041 gaaatggcag ataaaggttt aatttataaa ggtaaaaagc cagtttattg gtctccttca
    20101 agtgagtctt cattagcaga agcagaaatt gaatatcacg ataaacgttc agcatcaatt
    20161 tacgttgcat ttgacgttaa agatgacaaa ggtgtcgtag atgcagatgc taaatttatt
    20221 atctggacaa caacgccatg gacaattcca tcaaatgttg cgattaccgt tcatcctgaa
    20281 ttaaaatatg gtcaatacaa tataaatggc gaaaaatata ttattgcaga agccttatct
    20341 gacgctgtag cagaagcact ggattgggat aaagcatcaa tcaaattaga aaaagaatac
    20401 acaggtaaag aattggagta tgttgtagca caacatccat tcttagatag agaatcgtta
    20461 gtgattaatg gtgatcatgt tactacagat gctggtacag gttgtgtaca tacagcacca
    20521 ggtcacgggg aagatgacta tattgttggt caaaaatatg aattaccagt aattagtcca
    20581 attgatgata aaggtgtctt cactgaagaa ggtggacaat ttgaaggaat gttctatgat
    20641 aaagctaata aagccgttac tgatttatta acagaaaaag gtgcactatt aaaattagac
    20701 tttattacac atagctatcc acacgactgg agaacaaaaa aacctgtaat cttccgtgct
    20761 acaccacaat ggtttgcttc aatcagtaaa gtaagacaag atattttaga tgcaatcgaa
    20821 aatacaaact tcaaagtaaa ttggggtaaa acacgtattt ataatatggt tcgtgaccgt
    20881 ggcgaatggg ttatttcacg tcaacgtgtt tggggagtac ctttaccagt attctatgct
    20941 gaaaacggcg atattattat gacaaaagag acagtgaatc atgttgctga tttgtttgct
    21001 gaacacggtt caaatatttg gtttgaaaga gaagctaaag agttgttacc agaaggattt
    21061 acacatcctg gtagccctaa cggcacattt actaaagaaa cagacatcat ggatgtttgg
    21121 ttcgattcag gttcatcaca tcgcggtgta ttagaaacga gacctgaatt aagtttcccg
    21181 gcagatatgt acttggaagg tagtgaccaa tatcgtggtt ggtttaactc atcaatcaca
    21241 actgctgttg ctacgagagg tgtttctcct tataaattct tactttcaca tgggtttgtt
    21301 atggacggtg aaggtaagaa aatgagtaaa tctttaggta atgtgattgt acctgaccaa
    21361 gtggttaaac aaaaaggtgc tgatattgcg agactttggg taagtagtac ggactattta
    21421 gctgatgtta gaatttctga tgaaatttta aaacaaacat ctgatgttta tcgtaaaatc
    21481 agaaatacat taagatttat gttaggtaat attaatgatt tcaatcctga tacagatagc
    21541 gttcctgaat cagagttatt agaagtggat cgttacttgc taaatcgttt acgtgaattt
    21601 actgcaagta caattaacaa ctatgaaaac tttgactact taaatattta tcaagaagtt
    21661 caaaacttta tcaatgttga gttaagtaat ttctatttgg attacggtaa agatatttta
    21721 tatattgaac aacgtgattc tcatatccgt cgtagtatgc aaacagtgtt atatcaaatt
    21781 ttagttgata tgacgaagtt gttagcacca atcttagtgc atacagctga agaagtttgg
    21841 tctcatacac cacatgttaa agaagaaagt gttcacttag cagacatgcc taaagttgta
    21901 gaagtagatc aagctttatt ggataaatgg cgtacattta tgaatttacg tgatgatgtg
    21961 aaccgtgcat tagaaactgc tcgtaatgaa aaagttattg gtaaatcatt agaagctaaa
    22021 gttacgattg ctagtaacga taaatttaat gcatctgaat tcttaacttc atttgatgca
    22081 ttacatcaat tatttatcgt gtcacaagtt aaagttgtag ataagttaga tgatcaggca
    22141 acagcttatg aacatggtga tattgtcatc gaacatgcag atggtgaaaa atgtgaaaga
    22201 tgttggaact attcagagga tcttggtgct gttgatgaat tgacgcatct atgcccacga
    22261 tgccaacaag ttgtaaaatc acttgtataa ttgaaattgt ataaagtact catacagatg
    22321 atataaatta aagctctctt cataatcatg ttgtagtttt tattgacatg atgaagagag
    22381 tttttttgcg aataaaaaaa tgaccaagtt actggttata tgtgttaaaa aatgtgtaat
    22441 ttactaaaat aaaaattatt caggaatggt acaaattctc tgcagcttat aaatgcgtta
    22501 tagttgctat tctcaattat gttcgcgata attttaagta aaagtaagca cagatattga
    22561 atttgatagg agttaattga atgtatcata acagtaacgc aaactttgta aatggtatca
    22621 ctttaaatgt gagagataag aatgaattaa agccatttta tgaggacgta ttaggattaa
    22681 atattataaa tgagacatta acatcgatac aatatgaagt aggtcaaaat aatcatgtca
    22741 ttacacttgt tgaattacaa aatggacgtg aacctttaat gtcagaagcg ggactgtttc
    22801 atatcgcaat taaactacct caaattagcg atttagctaa tttactaatt catttaagcg
    22861 aatatgatat tccagttaac ggaggtatac agcctgcttc gttatcatta ttttttgaag
    22921 acccggaagg aaacggtttt aaattttatg ttgataaaga cgaagcgcaa tggacgaggc
    22981 aaaatgattt agtaaaaatt gatattagac cattgaatgt accgagatta gtgagtcatg
    23041 caacaaaatt gttatggtta ggtattccag atgacgctat tataggtgcg ttgcatatta
    23101 agacaattca tttatcagag gtaaaagact actacctcga ttattttgga ttagagcaat
    23161 cggcatatat ggatgattat tcaatatttt tagcatcgaa tggctattat caacatttgg
    23221 ccatgaatga ttgggtatca gcaacgaaac gtgtagaaaa ttttgatacg tatggattag
    23281 caattgttga ctttcattat cctgaaacaa cacatttaaa tttacaaggt ccggatggta
    23341 tctattatcg ctttaatcat atcgaagttg aagattagta tatactttga atggacgaac
    23401 catataatga atcgttttta atgatctttt tatacaagct atgaaggagc ttgagacatt
    23461 aagttcttag gcaatgtaaa aagctgattt ctattaatta ttcgatagaa atcagctttt
    23521 ttgatatgta ttttataatg tacagctcgt tgagct
//
LOCUS       DAHHRR010000031        22816 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.031, whole genome shotgun sequence.
ACCESSION   DAHHRR010000031 DAHHRR010000000
VERSION     DAHHRR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22816
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.031"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(145..528)
                     /locus_tag="NBI42_001054"
     CDS             complement(145..528)
                     /locus_tag="NBI42_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCD1777484.1"
                     /translation="MKERGHIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDDALLIEPSIGSVCNAFDHMWGYFKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            711..2153
                     /locus_tag="NBI42_001055"
     CDS             711..2153
                     /locus_tag="NBI42_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCD1777485.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLSDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIVAITVTLILSKRKANKEVVE"
     gene            complement(2238..3182)
                     /locus_tag="NBI42_001056"
     CDS             complement(2238..3182)
                     /locus_tag="NBI42_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCD1777486.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(3228..3851)
                     /locus_tag="NBI42_001057"
     CDS             complement(3228..3851)
                     /locus_tag="NBI42_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1777487.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGIPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIVFNGPLRIMAENRLHKSNLDSLT"
     gene            4012..5289
                     /locus_tag="NBI42_001058"
     CDS             4012..5289
                     /locus_tag="NBI42_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCD1777488.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQGFDFDHPNRIAIFVALAILGFVGLILGVMVWLGMKS
                     IPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            5495..5926
                     /locus_tag="NBI42_001059"
     CDS             5495..5926
                     /locus_tag="NBI42_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HCD1777489.1"
                     /translation="MRRLLYSFLFYMVIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            6045..6143
                     /locus_tag="NBI42_001060"
                     /pseudo
     CDS             6045..6143
                     /locus_tag="NBI42_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6157..7191
                     /locus_tag="NBI42_001061"
     CDS             6157..7191
                     /locus_tag="NBI42_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1777490.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVVSGDTHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            7227..7628
                     /locus_tag="NBI42_001062"
     CDS             7227..7628
                     /locus_tag="NBI42_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1777491.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTLEAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSQIMEHIMKYIKNVSVESAYV
                     SLIADYPADKLYAKFGFMPTEPDSGGMYIKY"
     gene            complement(7674..8678)
                     /locus_tag="NBI42_001063"
     CDS             complement(7674..8678)
                     /locus_tag="NBI42_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD1777492.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSTTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIVKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLKPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            8865..9380
                     /locus_tag="NBI42_001064"
     CDS             8865..9380
                     /locus_tag="NBI42_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1777493.1"
                     /translation="MAGIIKEISKQDASELVELATRTFYDTFGSYYDDKDFDQFFEDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGAHHFQTGDV
                     TDTDLIMEKEL"
     gene            9694..10323
                     /locus_tag="NBI42_001065"
     CDS             9694..10323
                     /locus_tag="NBI42_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777494.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDSNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKTQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(10476..12149)
                     /locus_tag="NBI42_001066"
     CDS             complement(10476..12149)
                     /locus_tag="NBI42_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1777495.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPLATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPR
                     SEKADELINSARVVLVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETAFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKRNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDETMLPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            12466..14064
                     /locus_tag="NBI42_001067"
     CDS             12466..14064
                     /locus_tag="NBI42_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCD1777496.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTVSEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            14444..15922
                     /gene="mqo"
                     /locus_tag="NBI42_001068"
     CDS             14444..15922
                     /gene="mqo"
                     /locus_tag="NBI42_001068"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCD1777497.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSVLFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFNNLEQRGVGIQYKQNVLDIKKQKSGA
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDILQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(15987..16442)
                     /locus_tag="NBI42_001069"
     CDS             complement(15987..16442)
                     /locus_tag="NBI42_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HCD1777498.1"
                     /translation="MKNLKNSLFISLIIGLTLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWGITKATIVHFITTYVGFLPLAI
                     LAGWFPLTINYLIIFTIIFIVVYTLIWIIQFFRNKIYVDTINEQLKQLK"
     gene            complement(16439..16882)
                     /locus_tag="NBI42_001070"
     CDS             complement(16439..16882)
                     /locus_tag="NBI42_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1777499.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDHVQSIINAVNDLDKSHTL
                     VGYIDKEIHIIHISDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYCIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            complement(17044..18417)
                     /gene="hssS"
                     /locus_tag="NBI42_001071"
     CDS             complement(17044..18417)
                     /gene="hssS"
                     /locus_tag="NBI42_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HCD1777500.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYITTFTI
                     TLPNNSH"
     gene            complement(18410..19084)
                     /gene="hssR"
                     /locus_tag="NBI42_001072"
     CDS             complement(18410..19084)
                     /gene="hssR"
                     /locus_tag="NBI42_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HCD1777501.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEKTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            19220..20275
                     /locus_tag="NBI42_001073"
     CDS             19220..20275
                     /locus_tag="NBI42_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1777502.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNVQTLKLSNKEQD
                     VITMNDVKQQRLQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FVKLFKVDPIEAIGGAE"
     gene            20275..20940
                     /locus_tag="NBI42_001074"
     CDS             20275..20940
                     /locus_tag="NBI42_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1777503.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSKRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMISQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(21171..22138)
                     /locus_tag="NBI42_001075"
                     /pseudo
     CDS             complement(21171..22138)
                     /locus_tag="NBI42_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            22333..22788
                     /locus_tag="NBI42_001076"
     CDS             22333..22788
                     /locus_tag="NBI42_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1777504.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
BASE COUNT         7830 a         3244 c         4075 g         7667 t
ORIGIN      
        1 gtatcttcta acatcatgaa acgcatttta atatagatat gtacatttaa accttgagag
       61 atagattata gtaaacttta gtacaaagta tgaattgctg aactccaatg actactattt
      121 gtccgttaca actaaagttt caatctatct ctttcttttt gtgtttaaaa cacgactttg
      181 tagtaaatat tgaacatcat atttattagc aagttctgct aaaaaatcta ataaagtttg
      241 ggtgtcgatt ttaccattaa taaaatcagc tttaagtagt tttgattgtt gtctttcttc
      301 ttcattcgca cattttttaa aatatcccca catatgatca aaagcattac atacacttcc
      361 tatactcggt tcaattaaca aagcatcatc aatcatttgt tggacttgat gaagtgataa
      421 atccgttttt aaagcattcc ttatcatttg gtaagactgt tgactgtgta acaatacatg
      481 atatttttcc tcacgccaaa gctgttcgat atggccacgt tctttcataa aattacctcc
      541 gataattaat gttaatatac atgcatatag tatagcaata aaaatgaatg cgctatcaaa
      601 aatggtgtag gtgtttatat aaaatgataa tagagtgttg caaatatttt tggaaccggt
      661 tacaataaag tggaataaat ggaaccggtt ctaattagga ggttaaatat atgaattata
      721 agcaatccgc agaagaaatt ttgaacgcga taggcggaga agagaattta gatgcaatgg
      781 cgcattgtgc aacgagacta cgattagttt taagtgatga aagtttagta aatgaagagg
      841 cgctaaacaa tatggatgta gttaaaggga cgttttctac tgggggacaa taccaaatta
      901 ttattgggtc tggtacagtc aataaagtat ttagtgaact ggaaaaatta actggaaaag
      961 aagcatcaac cacttcggaa gtcaaagcgc aatctgctaa aaatatgaat ccgttacaga
     1021 gatttgtaaa aatgctttca gatatctttg ttccgattat accagccatc gttgctggtg
     1081 gtttattaat ggggttaaat aacattttga ctgcgaaaga tttattcttt tcaggtaaat
     1141 cattgataga tgtatatagt caatttgctg gattagctga aatgataaat gtttttgcga
     1201 atgcaccatt tacattatta ccaattttaa ttggatttag tgcagcaaaa cgttttggtg
     1261 gcaatccgtt tttaggtgct gcattaggta tgatattggt tcatccatcg ctaatgagcg
     1321 catacgattt cccaaaagca gttgaagcag gtaaggctat tccatattgg gatgtatttg
     1381 gtttgcatat taatcaagta ggttatcaag gacaagtgtt acctatgctt gtagcagctt
     1441 atatcttagc ctcaattgaa aaagggttac gcaaagttat tccaacggtg ttagataatt
     1501 tgttaacacc attgttatct atttttataa cagcatttct aacattttca tttgtaggtc
     1561 caatcactcg acaattaggt tactggttat cagatggttt aacatggctt tatgaatttg
     1621 gtggtgcaat tggtggatta atattcggat tattgtatgc tccgattgtt attacaggta
     1681 tgcatcatag ctttatagct gtagaaacga cattaattgc agatgccact aaaacgggtg
     1741 gatcatttat attcccgatt gcgacaatgt ctaatgttgc acaaggtggt gcagcaattg
     1801 cagcgttctt tattattaaa caaaataaga agttaaaagg tgtggcatct gccgcaggta
     1861 tttcagcatt acttggtatt acagaaccgg ctatgtttgg tgttaactta aaactaagat
     1921 atccatttat tggcgctatc gttggatcag gtattggttc agcatatatt gctttcttca
     1981 aggttaaagc aatcgcatta ggaactgctg gattgcctgg atttatttca atcaatccag
     2041 tacatgcagg atggttacac tactttgttg gtatgacaat atcattcatc gttgctataa
     2101 cagttacttt aattttatcg aaaagaaaag caaataaaga agttgtagaa taaaaaagag
     2161 gtatgacagt aatcaaagta tcagtcattg caaatgcaat aggatgaaac tttaagctgt
     2221 catacctttt aatttagtta taatttctgt ttacgccata agaaaatact aacaattacg
     2281 acaagaatta atgaaatacc taccacaata atccaagata tataactatg gtcatcaatt
     2341 ggcaacggca cattcatacc gaagaaacta aatacgagcg ttggtaacgt taataggacc
     2401 gtgaaaagtg ttaatgtctt cataattgta ttcatatcat tagataataa ggaagcgtat
     2461 gatgttgtga tactttctaa gatacgttga tgtaattctg ttgtttcgat ggcttggtta
     2521 ttttcaatga ttaaatcttc tagtaattct tcatcttctt caaaacgttt aattgcaggc
     2581 aaacggaata actttttaat aattgtatca ttacctttta aggcagctaa aaagtatact
     2641 aaactttttt caacttccat taatttgaaa agttgtttgt tagtaatgtt attttttagt
     2701 tctttctcaa tacgaattct acttttatta agtaatcgta agttacgatt ataatggtcg
     2761 gcaatagtag ttaaaatttc taatgcaaat ctgctgtgaa attttaaatt aatatcacgc
     2821 ttaggtaaat tttctaaaaa ttcattttca gcgtcacaaa ctgttacgat aatgccgttg
     2881 ccaataataa tgcctaatgg aatcgttaca aaagatagaa cgctacgatt agttgaattg
     2941 acgataggta aatcaataat gattaatgag taaccagtat cttcgtcata ttcaatacgg
     3001 gaactttctt ctgagtccaa agggtcacgt ataaagtctt cgggtatatt atattgttcc
     3061 ataagatttt caatttcttc tcggtctggt tctactacat taatccatga cgctgtgtga
     3121 tctaatggtg tttttataat atgtttatct atatcttcag aatgtctgaa tgaagtaatc
     3181 atgatatata gttcctttct aaaggattta ggaaaagcga gtattactta agttagtgaa
     3241 tcaaggtttg atttatgcaa gcggttttct gccattattc ttaatggacc attaaaaaca
     3301 atattatgca catgtttagc taattcatca actgaaatgg gctgtttgtc cattacccag
     3361 tatttaataa ttgaaattgt tgctccggaa acgtaactgt aaaaatacat ctctggaatg
     3421 cctccgatag aatgattgat actaatataa cgttgcatat tatttttaat atgttcgttg
     3481 attttcaatt ctaattgact cgtgcgttcc aattgtaaaa tagtatgata aaactctaaa
     3541 ttatcatgaa tatgttggaa aatatttttg agtatattat ttacaaaagt attcgcaatg
     3601 tcttcaattg aagaaccgcg taacaaatca aattgagtat atgtcgttag ttctgaaata
     3661 tactcatctt ccatatctgc cagtaacata tatttatcct cgtaatgtaa gtaaaatgtc
     3721 cctctattga tatcagcgcg tgttgttatg tcgcgaatgg taatcttttc taaatctttt
     3781 tcttgtagta atttagtgaa cgcttgttta atggaggact ttgtctttct aattcgccta
     3841 tcttctttca aaaataaatc aacctccttg tgttattgaa caatgtgggt gtatttgtct
     3901 attaataaac gtattgatga acattgtgta ttgtttattc acacttacat attataatag
     3961 acaacatgcg aaatattcaa cacattgtct aataaggagg taattaataa aatgaatata
     4021 tttaaaaata aattattatg gattgcacca atagcaacta tgattatctt ggtaatcttt
     4081 tctttagctt tttatcctgc atataatcct aaaccaaaag atttaccaat tggtatatta
     4141 aatgaggata aaggtacaac gattcaagat aaaaatgtta acattggtaa aaaattagag
     4201 gataaattat tagatagtga ttctaataaa attaaatggg ttaaggttga tagtgaaaaa
     4261 gatcttgaaa aagatttaaa agatcaaaaa atctttggag tagctattat tgataaagac
     4321 ttttcaaaag atgctatgag taaaacacaa aaagtagtta tggatagtaa aaaagaagag
     4381 atgcaacaaa aagttgcttc aggtgaaatt ccgccacaag tagttcaaca aatgaaacaa
     4441 aaaatgggga atcaacaagt agaggttaag caggctaaat ttaaaacgat tgtaagtgaa
     4501 ggatcaagct tacaaggttc acaaattgca tcagctgtgt taactggtat gggtgataat
     4561 attaatgctc aaattacgaa gcaaagtttg gaaacattaa cgagtcaaaa tgttaaagtc
     4621 aatgccgcgg acatcaatgg tttgacgaat ccagtaaaag tggataatga aaaacttaat
     4681 aaagttaaag atcaccaagc aggtggtaat gcaccattcc taatgtttat gccaatttgg
     4741 ataggttcaa tcgtaacgtc catcttattg ttctttgcat ttagaactag taacaatatc
     4801 gtcgtgcaac atcgtatcat tgcttcaatt ggacaaatga tatttgcagt tgttgcagca
     4861 tttgcaggta gctttgttta tatttatttc atgcaaggcg ttcaaggatt tgattttgac
     4921 catccaaatc gtatcgcaat ttttgtagca cttgcgattc ttggtttcgt gggccttatt
     4981 ttaggtgtta tggtatggct aggtatgaag tcaattccaa ttttcttcat tttaatgttc
     5041 tttagtatgc aacttgtaac gttacctaaa caaatgttgc ctgaaagtta tcaaaaatat
     5101 gtatatgatt ggaatccatt cacacactat gcaacaagtg taagagaact attgtacttg
     5161 aatcatcata ttgaattaaa tagtacaatg tggatgttta tagggtttat gatttttggc
     5221 gctgtatcaa gtttagtttc agctattgtt agaaaacata gcacaaaacg tactgaagtg
     5281 ccgtcataat ttaataaata ggaatatagc tatacaagac gtttgtagcg agcgtcatgg
     5341 tatagctatt ttatttgtat agcgctggat gtaaagttat tttagctgtc atactatgtg
     5401 ctaaaacaaa ttcagttgtg tcatatcttc gcttttttgt cactgttata ataggtataa
     5461 tcggatataa tgaaaaggtg agggaggatt caaaatgcgt aggttattat attcatttct
     5521 tttttatatg gtcataggtt tatttagtgg ctttttctat agagagctaa caaaagcata
     5581 tgattttact ggtacaacac aattatcact tgtacacaca catacactta ttttaggtat
     5641 gtttatgttt ttaatattat taccattgga gaagttattt aaattatcaa gttactactt
     5701 atttaactgg ttcttctatg tatataacat aggtgttatc gttactatag gtatgatggt
     5761 gacaaaagga ttcttccaag tcactggtaa atcatactct ccagaagcat ttgcaggctt
     5821 tgcaggtatt gggcacacag gtatgcttgc aggtttattg ttgctatttt tccttttaag
     5881 acaagcaatt cttaaagaac cacgtgatga gtcttttaaa aaataatata actagaacta
     5941 aaaaatatac acatctttca aagaaaacaa ttttgtttgg agctttggaa ggtgtgtttt
     6001 ttatagtgat gaaatcattt tgatatttca aagctaacta tgcgatttat tttaacaatg
     6061 caagctttaa tagtataatg gttttaaatg ataattatta tcaatttgag tgcgttaatg
     6121 aaagaaatga atatagagaa acacaggagg cacataatga aagacgtaac aatcattggt
     6181 ggcggtccgt ctggtttgta tgcaagcttt tatgctggat tgcgagatat gtctgtaaga
     6241 ttgatagatg ttcaatctga attagggggt aagatgagaa tttaccctga aaaaattatt
     6301 tgggatattg gcggcattgc gccaaaacct tgtcatgaaa ttttaaaaga tacaattaag
     6361 caaggattat attttaaacc ggaagttcat ttgaatgaac gtgtggtaga tattaggaaa
     6421 aaggcagaac gtcatttcga agttgaaaca gaagcgggag aaatatatac atcgaaagca
     6481 gttatcattg ctattggtgc gggtattatt aatccaaaac aattagatgt taaaggtgta
     6541 gagagatatc aattaactaa tttacattat gttgtacaaa gttacagacg tttcaaagat
     6601 aaagatgtat taatatcagg tggaggaaat acagcattag attgggcgca tgatattgcc
     6661 aagattgcta aaagcgtgac agttgtttat cgaaaagaag atgtaagtgg tcatgaagcg
     6721 atgaaaacgc tggtgacaga tttaaatgtg aaactatgcc cgaaaacacg tattaaatac
     6781 ttggtcggca atgatgatga aacgcatata agtgaagttg ttttggaaca tgttgtaagt
     6841 ggtgatacgc atacagttaa atttgacgat gtcattatta gtcatggttt tgatcgttgt
     6901 aatacattat tgagtgaaac atcttcaaag ctagatatgc atgatgattg tcgtgttaaa
     6961 ggatttggta atacgacaac aagtatacct ggtatctatg cgtgtggaga tattgtttat
     7021 catgatgcaa aatcacatct aattgcaagt gcatttagtg atggtgcaaa tgcagcgaac
     7081 cttgctaaaa catatatcca accagatgcg aatgcagagg gttatgtttc aagtcatcat
     7141 gaagtgttta aagaagcgaa taagactatt gtaaataaac atttatacta ggttgatata
     7201 ttgtgtgatg aaaagtgggg taaataatgg ttaaagtgac atatgatatt ccgacttgcg
     7261 aggattattg cgcattaagg attaatgcag gtatgagtcc aaagacgctc gaagcagctg
     7321 aaaaaggatt acctaatgcc ttatttacag taaccttgta tgataaagat cggttaattg
     7381 gtatgggtag agtgattggc gatggaggaa ctgtttttca aattgttgat attgcagttt
     7441 tgaaaagtta ccaaggtcaa ggttacggca gtcaaattat ggaacatatt atgaagtata
     7501 ttaaaaatgt atctgtcgaa agtgcttacg ttagtctgat cgcagactac ccggcggata
     7561 aattatatgc aaaatttggg tttatgccta ccgaaccaga ttcaggcggt atgtatatca
     7621 aatactaaat taaaaaagac atctcgcata acgggatgtc tttttcacga atactattta
     7681 tctacaccga aatcgataac aatgcggcct ttattttcat gtttaattac tttgttaagt
     7741 tgttctggaa gttcatcaaa tgtaataact tgcttgatct catgtaaatt ttcaggcttt
     7801 aaatcttttg cgagacgacg ccaaacgcgc tgtcttaatt tcatagcagt aaatactgag
     7861 tcaattccta aaatgtttac acctcttaaa atgtgaggga atacagaatt tgtataagta
     7921 ttaccggcag tcataccaat aactgcaatt gatccactat gatttaaacg ttttgtaaca
     7981 taattaatac cttcgccacc aacagggtct acacaagctt gccaagttga tgatgcgagt
     8041 ggctttttat gatcatcttc aacaggaagt cgatcgataa cttctttggc accaagttca
     8101 agtaattgat cgctaacatc ttgtttacct gtacttgcga taactttata accaagttca
     8161 ttaagcatga gtactgctaa agtaccgaca ccacctgaag caccgcgaac gagtacagga
     8221 ccatcttcaa tattcattcc aactttttca agtctttcaa ttgctaaacc ggcagtataa
     8281 ccagctgtgc catatatcat tgattcttct aatgttaaag tatcaggaag cttgacaatc
     8341 cattctgatt ttacacgcgc atattcacta aaaccgccat aatgactgac acctaggtca
     8401 taactcgtta caattacttg ttctcctttt tcaaagcctg gtgcttcgga ttcaacaatt
     8461 gtaccagcta aatctattcc tggaatcata ggatacgatt ttacgactgc attatgatct
     8521 tgagtcgcta aagcatcttt ataatttata ccagagtaat gtactttaat cagcacatct
     8581 cctttaggta aatcagtagt cgataattgt ttgaaagttg gtgtcacttt accgctctca
     8641 tctttatcaa ttacaaacgc tttaaatgtt tctatcacaa cgatacatcc tctctttttc
     8701 tcttttgaaa atacggccat tttactggaa taaatgtatc atttattgat gttacacaca
     8761 atcaatacaa tttgtaattg atttgtgtta atgtattttt aaatagtttt ggctgaaaaa
     8821 ttaagtaata ttttgattcg atcactgttt attggagggt ttatatggct gggataatca
     8881 aagagatttc aaaacaagat gctagtgaat tagttgaatt agcaactaga acattttatg
     8941 acacgtttgg ttcttactat gatgacaaag attttgatca attttttgaa gataattata
     9001 ctgtagaaaa atttacacaa gagattaacc atgtagattc atttcattat ttttatcaag
     9061 aagatggtgc gaatgttggt tatataaaga tgaatattaa tagtgctcaa actgaagaaa
     9121 tgggggagac ctatttagaa gtgcagcgca tatacttttt gaaagacttt caaggtggcg
     9181 gaagaggttc acaattgata gaattagccg aaaaaattgc acaagaacat aataaacata
     9241 aaatttggct aggagtttgg gagcataatc ctcgtgctca agcattttat aaacgtcatg
     9301 gatttaaagt ggttggtgca catcattttc aaacaggtga tgtaacggat acagatttaa
     9361 ttatggaaaa agagttataa tgattgtttt tgcagcagat aaaatgaaat catagtaata
     9421 aatattgtgt ataccttgaa atgctaaatg cgtaatgaat tgttaaagtg aaataattaa
     9481 atttatagaa aacataaaat tcacaaaaat taaataataa tgaataaaaa ttagggtata
     9541 ataaaaagaa ccttagattg tagttatggg tattgtgatg attcaattca tattaatgtt
     9601 gacgtctttg gtaacaagcc atgaaatgat acacatggta gatatgtatt tcaagcttat
     9661 tcaatgaata tcgaattata ggaggagata tgtatgaaaa gattagttac agggttacta
     9721 gcattatcat tatttttagc tgcatgtggt caagatagtg accaacaaaa agacagtaat
     9781 aaagaaaaag atgataaagc gaaaactgaa caacaagata aaaaaacaaa tgattcatct
     9841 aaagataaga aagataataa agatgatagt aaagacgtaa acaaagataa taaagataat
     9901 agtgcaaacg ataaccagca acaatctaat tcaaatgcaa caaacaatga ccaaaaccaa
     9961 acaaacaata accaatcaag taataatcaa gcgaataata atcaaaaatc aagttacgtt
    10021 gcaccatatt atggacaaaa tgccgcgccg gttgcacgtc aaatttatcc gtttaatgga
    10081 aataaaactc aagctttaca gcaattgcca aatttccaaa cagctttaaa tgcggctaat
    10141 aatgaagcaa ataaatttgg tagtaataat aaagtgtata atgattattc tattgaagaa
    10201 cataatggca actataagta tgtgtttagt tttaaagacc caaatgcaaa tggaaaatat
    10261 tcaattgtaa cggttgatta tactggacaa gcaatggtta ctgatccaaa ctaccaacaa
    10321 taatgttaat ataactacga tgcaagttaa aaaattaaaa cggtaaactc tataatatga
    10381 attagggttt accgtttttg cgtattttaa agtataaagg tttacgttct atagtacttt
    10441 taaacctcat tcacagccac tgaggaattt aaacattatt gattagatgt catcttagac
    10501 aatataagtt gtgaaataga ttctaatggt tgttctttat gttgtatttg tggatattta
    10561 atcactggta ttgcctggga aattaaatta ttaattaata attgtcggtc tgtataccaa
    10621 atttttgata gatgacgcca aatatcttcg ttaacacctt gagactcaat atattgactg
    10681 tgatttggag tgtataatgc tactgttttg tctatagcca tcgcatcaaa tattatattt
    10741 gagtaatcag tgataacgac atcagacatt agaattaagt cttgagcact aagatacttt
    10801 ggtgcaacaa gcgcctcttc tggtaacatc gtttcatcaa caccctgaac cacaacatga
    10861 taggctttga ataaagcatc tgatagtggt aattgttgcg cactcactaa tccaataggt
    10921 gcgtataata aaactggttt ttgatcattt attttaaacg atttcttata ctgttgttga
    10981 gtagtacttt cgttacgctt ttgtagtaaa tattggtgtt taggattacc ataagccaat
    11041 acatttgctt ggtgactagg aaaagccgtt tgataaaagt ggctaatatc atcagcagaa
    11101 tgtataacat aatcgaattg caaccagcga ttataccttt tagcacgata aaaagctgtt
    11161 tctatatttt taataggctc tttactatct aaaaataatt gcattattgg tgtgccttga
    11221 tgtaattgaa ttaaagtacc gtttggttgt aacgtttcag gtatatcatt ttcaactaaa
    11281 actacgcgtg cgctgttaat taattcgtca gctttttcag acctaggtga tatgaaatgt
    11341 ggtccgcgac gatcgtcagt aacaaaataa caggcagtca ttggattatg tttaacgaag
    11401 taagtaaata aatattttga attacctata tattgatgat gatcaccgat aaaaacgatg
    11461 tgatctttta aaacgtttgg ttgcttcgta tacaagccga agttttgtaa ttgtttgtta
    11521 tcagcacgtt gctttgctat tttatataac catttaggta gcataaactt ggtagaatac
    11581 aaccaattat ctggcgaatt aagttgttct gaatcctcga taaaaaggtt gcgaaaaggt
    11641 ataggatgat gatcaatata agccatttta ttcaaataaa actgagacat catatcaaaa
    11701 tcttcaatag ttttaatagt ttcccatatt gaagcacttg ttgctaaagg ggctttaaaa
    11761 tcaaaaacga catgaccatc gattgataaa tcacctttca gaagtgtcaa taaagctaaa
    11821 tcgaaaatcg tttctacaga gtaataatgt attaaatcaa gagtctgttg aatcgcatat
    11881 atcaggttag gataataatt attgttttga gcccaatcgg caatgttgat cgatgattga
    11941 ccgaaatagt gacaatcatt tttgtattgt ccatccacag tataatcaac cacgatagac
    12001 ttaggatata attgaaccgc atctaacata gattgataaa tcattatctc attggcatat
    12061 ggaacgacat gtgtataacc ttcagactga gctttcatta attgagattc aaacttttgc
    12121 atattagaaa catttatctg tttaatcatt gctataactt ccatttctca tcgtagtatc
    12181 ttccttattt tagcacggtc ttatgtaaaa ttcatgaata gtataagtta cttcattaca
    12241 ttaagtacaa ttcattttga tgaacagatt taaaatgaaa aataaaacaa ttaaatagga
    12301 aagtgatatt gttaatagtc tgaaaaaatg gtatatttta attgtgaaaa aaatcacatt
    12361 aatctcactg ttctgtaaaa tgttatcgat aacaaaatgc tatagccaga gcatgatttt
    12421 aatgaatcaa ttacactact actttatgga aagaagagtc agcctatgtt agtaaatacg
    12481 tttaacccat ttgataattt attattatcg tctttaattg cagcaatacc gatagtactg
    12541 tttttacttt gtttaactgt ttttaaaatg aaaggtattt atgcagccat tacaacactt
    12601 gttgtaacat tactaattgc aataccattt ttcaaattgc cagttggtat cgcttctggt
    12661 gcagtagtcg aaggtttctt ccaaggtatc attccgattg gctatatcgt tatgatggca
    12721 gtattgttat acaaaattac tgttgaatct ggacaatttt taacaattca agatagtatt
    12781 acaaatattt cacaagacca acgtattcaa gttttactta ttggatttgc attcaacgca
    12841 tttttagaag gtgcagcagg atttggtgta ccaattgcaa tttgtgcact tttattaaca
    12901 caattaggat ttaatccatt aaaagctgcg atgttatgtt tagtcgcaaa tgcagcgtct
    12961 ggtgcttttg gtgcgattgg tatccctgta ggtgttgtag aaacgttgaa attacctgga
    13021 gatgtttcag tattaggtgt ttctcaatca gcaactttaa cattggcaat cataaatttc
    13081 attattccat tcttacttat ctttattatt gatggtttta gaggtgttaa agaaacatta
    13141 ccagcaattt tagttgtttc aatcacttat acacttactc aaggattatt aactgtattc
    13201 agtggacctg aattagcaga tattattcca ccgttattaa caatgttagc attagcagta
    13261 ttttctaaaa aattccaacc aaaacacatt tatcgtgtta ataaagatga agaaattgaa
    13321 cctgcaaaag cacattctgc aaaagcagta ttacatgcat ggagtccgtt cattgtatta
    13381 acagtcattg taatgatttg gagtgcgcca ttctttaaaa acttattctt accaaatggt
    13441 gctttatcat cattagtatt taaattcaac ttacctggaa cagtcagcga agttacgcat
    13501 aaaccattag tattgacttt aaatattatt ggacaaacag gtacagctat tttactaact
    13561 attattatta caattttaat gtctaaaaag gttaacttta aagatgcagg tagattattc
    13621 ggtgttacat ttaaagaatt atggttacca gttcttacaa tttgtttcat cttagcaatt
    13681 tctaaaatca caacttatgg tggtttaagt gcagcaatgg gtcaaggtat tgctaaagca
    13741 ggtaatgtct tcccagttct atcaccaatc ttaggttgga taggtgtgtt tatgacagga
    13801 tcagttgtaa ataacaactc attatttgca ccaattcaag cttctgttgc acaacaaatt
    13861 ggaacaagtg gttcacttct tgtatctgct aatacagttg gtggtgtagc ggcaaaactg
    13921 atttcaccac aatcaattgc aattgcaact gcagcagtaa aacaagttgg taaggaatca
    13981 gaattattaa aaatgacatt gaaatacagt gtatgtttac taatattcat ctgtatttgg
    14041 actttcatct tgtcattact ataaataaac gtatttcaaa atataaatat acagagggtg
    14101 agatgttttc taacatctca tcttttttaa tggatcatta atgaaagaag tttgtcatta
    14161 taataatggt agcgctttat gttaaaatga agaatgagta atcagcaatc aaattaaatt
    14221 ggttgatagc tgttaaggtt tgtggttttg tctttgtgct atcgcacata aagtatataa
    14281 ttaaagtagt ttcgttatta taaaatattt atatacatag tagatagtaa tagagcaaca
    14341 ccatgggaac ctattgagac acttattgat tcaaagtggt attaatatgt cgtatttctc
    14401 gaacgttcca ttattcattt taaaaagggg gactgtattt gttatgacaa cacaacatag
    14461 caaaacagat gtcatcttaa ttggtggcgg tattatgagt gcaacattag gaacattact
    14521 taaagaatta tcacctgaga aaaatattaa agtgtttgaa aaattagcac aacctggcga
    14581 agagagttca aatgtatgga ataatgccgg tacagggcat tcagcacttt gcgagttgaa
    14641 ctatacaaaa gaaggtaagg atggcacagt tgattgtagt aaagcaatta agataaatga
    14701 gcagtaccaa atttcaaaac agttttgggc atatttagtt aaaacaggac aattagataa
    14761 cccagatcgc tttattcaag cggtgccaca catgagtttc gtcattggcg aagataatgt
    14821 agcttttata aaaagtcgtg ttgcaacgtt aaagaaaagt gttttattcg aaaaaatgaa
    14881 attatcgcaa gatgaagaag aaatgaaatc ttgggtaccg ttaatgattg aaggtcgtaa
    14941 gtctgatgaa ccaattgctt taacttatga tgaaactggt acagatgtta attttggtgc
    15001 gttaactgca aagttattta ataatttaga acaacgtggt gtgggaattc aatataagca
    15061 gaatgtatta gacatcaaga aacagaaatc cggggcatgg ctagttaaag ttaaagattt
    15121 agaaactaac gaaacgacaa catatgaatc tgattttgta tttattggtg ctggcggtgc
    15181 gagtttacca ttactccaaa agactggaat taaacaatca aaacatattg gtggtttccc
    15241 agtaagtgga ttattcctgc gctgtacaaa tcaagaagtg attgatcgtc atcatgctaa
    15301 agtgtacgga aaagcagcag tgggtgcgcc accaatgtca gtgccgcact tagatacacg
    15361 ttttgtagac ggcaagcgtt cattgttatt tggtccattt gcaggtttct cacctaaatt
    15421 tttaaaaaca ggttcacata tggatttaat taaatcggtt aaaccaaata atatcgtgac
    15481 gatgttatct gcaggtatca aagaaatgag tcttacgaag tatttagtgt cacaattgat
    15541 gttatctaat gatgagcgta tggatgattt aagagtcttt ttcccaaatg ctaaaaatga
    15601 agattgggaa gtgattacag cagggcaacg tgtccaagta atcaaggata ctgaggattc
    15661 taaaggtaac ttacaatttg gtactgaagt cattacgtca gatgatggca cattagctgc
    15721 attacttggt gcatcacctg gtgcgtcaac agctgtagat attatgtttg atattttaca
    15781 gcgatgctat cgtgatgaat tcaaagggtg ggaaccaaag attaaagaaa tggtgccgtc
    15841 atttggttat cgcttaacag atcatgagga tttatatcac aaaattaatg aagaagtaac
    15901 taagtattta caagttaaat aataaacaaa acggtaatgt ctttttaatg tgatagacat
    15961 taccgttttt tagttgttaa taaaaatcat tttaattgtt tcagttgctc gttaatagtg
    16021 tctacgtaga tcttgtttct aaagaattga ataatccaaa tcaatgtgta aaccacaata
    16081 aagataattg taaatatgat taggtaattt attgttagtg gaaaccaacc ggcaagtatt
    16141 gctaaaggta agaatccgac atacgttgtt atgaaatgca ctatagttgc tttagtaatg
    16201 ccccaatctg tgtatttaaa gataaaatct ccaaggaaaa agacgacgcc tatgagtaac
    16261 cataaaatga tagaaatcaa cattacggta gtttctgtga aatgcgtata atacaatatg
    16321 ccaatagttg attgtgggtt cagtggataa tatttgccgt ctgcaaataa catactaaag
    16381 aacagtgaaa gggtcaaacc aatgattaag ctaatgaata atgagttttt caaatttttc
    16441 atattgataa gcgctccttt atagatttta aataacgtct agaagaatag gtgtagtgtg
    16501 catctttaag atacatacgt ataagtccat ttggctctaa taataatttt tcaatgcaat
    16561 acttgttgac gatttctgat ttggaaatgc gaatgaaatg ttgtggtaat tgtttttcta
    16621 gttcataaag tcgtaatttt agtttgaatt tttgattgct tgtaatggct gttacatttt
    16681 tattaataac ttggaatgtt ataacatctg atatatgaat gatatggatt tctttgtcta
    16741 tgtatccgac taatgtatgt gatttgtcta aatcattgac tgcattaata atactttgaa
    16801 cgtgatcatt cattttaggt gcatgtatat caatataaga ttccgtctca tttgcattga
    16861 taaataaatt gagtttcatc ataggttaat gcctccttca aaattattaa accataaacg
    16921 accatcgata tatttaaaat gtgttgaatg gtagaaatta aatgttaagt ggctagaaag
    16981 cgctaatcaa tataaaagat acctcctgaa ataaaaacag aaatgatttt caggaggtag
    17041 agattaatgt gaattatttg gcagtgtaat agtaaaggtg gttatatact cgttactttg
    17101 tgtgaattgg attgtaccat gatgcaattc aatgatggat tttgtaattg caagacctaa
    17161 gccattgcta ttatcatgtt tgctcacttt ataaaaacgt tcaaataaac gtgcttcagc
    17221 ttgtggacta attggtgaac catcattact tattgtgaaa atgatattgt tgtgactatg
    17281 ttgcaaagcg atgtcaatgg caccaccaac atctgtatac ttaatagcat ttattaataa
    17341 attactcaat gcttgatgta acaaacgttg atttcctagg aaattgattg attctaggtc
    17401 agctaaaatg attaacgact tttcatcagc agcaaattgt tcatgtcgaa tgatatcttt
    17461 aatgagttga ttgacttgaa tcttatcatc gaataataaa tgttggtgat tatctaattc
    17521 ggataagagc aacaattcag ttgttaatcc gctcagctgc gttgtaatgg tatagatatc
    17581 atttatatat tgttgtctta atgttttatc agaagtttgt tgtaactcgc ttaataaatg
    17641 atgaatatgt gttaatggtg ttttgatttc atgagataca ttttgtacaa aatgctgcct
    17701 catttgatca acttgtccca atgactctct catcttatta aagtgatact gtaatgttcc
    17761 aatttcatct ttgcgtgttt gtttgatagg tgtttcaaaa tcaccatcaa ttaagcgttc
    17821 ggtcgctagc tttaactttt ttactggacg aatgattgaa taggttgatg cgataactaa
    17881 tgaaatcgag ataaataata acaacattaa caagacggct aagaatgttc taaactcgct
    17941 aaatgtttct ccaatatctg gacgcataaa tactgctatt gaaccgtctt ttgtcttaaa
    18001 gttaatacca acagtattat ctgttacatt gtcgaaaaat ccagttacga ataatgcgaa
    18061 tggtttatct ttaataccat ggtaatcttg attatttaat acgttgttaa tagcattttg
    18121 tgataaagta tcttctctaa atggctcccc ataaaatgtc ttatgacctt tttgatcaat
    18181 ggtcattatt tggtaattca tttgccctaa atgtttgaaa tattgttgaa tatgcgttgg
    18241 tttagctgat tgttcatatt gtcttgcttc tttaagcgtc ttcatgattt tcgcgtcatt
    18301 agatgctttt aaattataat gatagtaaac atttgttaat acaaaactta ttaatgcact
    18361 aaataaaata accgtaatgg aataaatcgc aattctagca tagagtgttt taaacatgat
    18421 tctccacctt atagccttgt cctcttactg tttcaattgt aagtgtggca tttaattttt
    18481 ttaatctttg gcgtagtcgc ttaatatgaa cgtcaactgt tcgttcgtct ccttcataat
    18541 catagcccca aattttttct attatctgtt cacgagtaaa gatttgctta ggacgtgctg
    18601 caagcataaa taataattga aattccttgt tcggtaacgt catcgtttta ttagatactt
    18661 ggagttccaa ataggattgg tttagcgtta agttaccaat ggtcttttct gaatttgaat
    18721 tgatattata tcgacgtaat accgcacgaa ttctaaaaat aagttcctta acctcaaagg
    18781 gtttggttac ataatcgtca gtaccactta taaacgcacg ctctttgtca ctaagtgcat
    18841 cccgcgctgt taacataata actggtatat cataatcatt ttttaatgta ttacataatt
    18901 gaaagccgtc cataccatcc atcataatat ctaccactgc aatatcgaca cgctgttttt
    18961 ctaaaagttt taatgcagct tcaccacttg gttgtgtgta tgcatcaatg tgctctgttt
    19021 gtaaatggct agctatataa tttaggattc gaggatcgtc atcgacaaca agacattgca
    19081 ccatagctat aaactccctt atctttttca tttattatac atgtaaaata tttttgcgta
    19141 aaaaaacaat tgttcatatt gagttcatat ttcaacctta tactgacgct aaagaagaaa
    19201 tagggagaag tgaatcgata tgaaattagc gataaaagag attatgtttt acaaatttcg
    19261 ttatatttta atcacattaa tcattctttt attaagtatt atggtgttat ttattagtgg
    19321 tttagctcaa gggcttggta gggagaatat ttcgttattt gaacactttg ataatgatga
    19381 atatgttgtt caaaaaatga aagagccaca aattgagaaa tcgcaactta gtgatacgca
    19441 acagaatcaa attaaaaagg taattcatca agaaccatat aaaatgaatg ttcaaacatt
    19501 gaaattatct aataaagaac aagatgtgat tacaatgaat gacgttaaac aacaacgctt
    19561 acaacttaaa aaaggtgatt atcctaaaaa tgctcatgag gtcgctatta atgataagtt
    19621 agctgcagac aacattagag tcggggatag attacatttt aaaaataatt caactagtta
    19681 tagagtttct ggtattttaa acgacacaat gtatgcgcat agttccattg tgctattgaa
    19741 cgataacgga tttaatgcat tgaataaggt taatacggca ttttatccag tgaaaaattt
    19801 aacgcaacaa caacgtgatg agcttaataa aataaatgac gttcaagttg tgagtgaaaa
    19861 agatttaaca ggtaatattg cgagttatca agcagagcaa gcaccgttaa atatgatgat
    19921 tgttagtttg tttgctatta cagcaattgt tctaagtgca tttttctatg ttatgacgat
    19981 tcaaaaaata tcacaaattg gcattttgaa agcaattggt attaagacaa gacatttatt
    20041 gagtgcgtta gttttacaaa ttttaacact aacaataatt ggggtaggta ttgctgtgat
    20101 catcatagta ggactatcat ttatgatgcc ggtaaccatg cctttttact taacaacgca
    20161 aaatatttta ttgatggtgg ggatatttat attagtagcg attttaggtg cctcactatc
    20221 atttgtcaaa ttatttaaag tagatcctat cgaagcaatt ggaggtgcag aataatggca
    20281 ttagtcgttg aagatatcgt caaaaatttc ggagaaggtt tgtctgaaac aaaagtttta
    20341 aaaggtatta attttgaagt ggaacaaggg gaatttgtca ttttaaatgg tgcctctggc
    20401 tctgggaaaa caacattgct aacgatatta ggcggattgt taagtcaaac gagtggtaca
    20461 gtgctttaca atgatgcgcc attgtttgat aaacagcatc gtcctagtga tttgcgattg
    20521 gaagatattg gttttatttt tcaatcttca catttagttc cttatttaaa agtgatagag
    20581 caattgacac tcgtaggcca agaagcggga atgaccaaac aacaaagttc gaaaagagca
    20641 atacaacttt tgaaaaatat tggtttagaa gatcgcttga atgtatatcc gcatcagtta
    20701 tctggcggtg aaaagcaacg tgttgcgatt atgagagcat ttatgaataa tccgaaaatc
    20761 attttagcag atgagcccac agcaagttta gatgccgata gagcaacaaa agttgttgag
    20821 atgataagtc aacaaattaa agaacaacaa atgattggta ttatgattac acacgatcga
    20881 agattatttg aatatgcaga tcgagtgatt gaattagaag atggcaaaat aactgattag
    20941 tggcttgcaa agacgctaaa tgttaatgat ttaagacata gtagtataaa agttagataa
    21001 cagaatatga tttgggtcta caaaaaagca cgtcactatt atataaaata atgacgtgct
    21061 ttagtttatt tattttcaat ataacaattg tctaatcaag gattaacgaa tatttaaaga
    21121 tagtttgacg caatattaga aacaacatat aatgatagtt tgtttgtgga ttaactatta
    21181 taaataaaag cggcgtaaag acatataaac caactacttg aacaatataa cgttagtaac
    21241 aatctatact gatacattac gcctagataa tctttaatga gcacatgtaa gaaaaagtga
    21301 tatggtgtat gacttccgac accatcgata gataaaccta atttttgggc tagtcgtaag
    21361 gcgcgtaata catgaaactg acttgttaca caaacaattt taactgcttc atgatacaaa
    21421 ttgttgatga tttgtttaga atataaaagt ttgtgtatgt atttatagag tgagattcca
    21481 ttagtatatc tgttttatca acaccatgtg caattaaata acgttgcata gctaaagctt
    21541 caggaattgg ttcgtctggt ccttgtccgc cagatacaat aatctttgtt gctgatgctt
    21601 gttgttgata gatatcaagt gcacggtcta aacgcgctgc aagcattggt gtgacaaatt
    21661 cggtaaaaat accagcacct aacacaatta tgatatcgac ttctttgttg tatgatctat
    21721 gtctatatga tactgtccaa atgagataac aaataaaggt tagtaacagg gaaaggcata
    21781 atatagctaa ccacatagac aaacctttca caatgggtga ctgaatcgta cttataaata
    21841 gaagtgctga tgtgtagagt acaaatttat atgaaaaaga taataatttt ttaataaata
    21901 agcgactaga agtatgagaa aataaatatc tatgtttgaa tagcatgata atactgatta
    21961 ttataaatgt tacaaacata gaccaaggga aagtataggt catgatgcta tagatgagtg
    22021 acaaaaatat cgatatgaca actaagatgt agcatgttaa atttaacgcc agagtatagt
    22081 tgaaaattaa cggacaaata acgataagta taaatattaa taataaattc aataacatac
    22141 tgacacctcg cttataataa atattaaata taaatgtaga tgatttaatt tattaaagca
    22201 aggagaacgt agcaacatgt aagtcttaat ttgttatatt atatatgggt caatattttt
    22261 gtgtttttta gatgtgcgat acaatgattg ctgaggatac tttataaaaa tagtaattag
    22321 ggagaaataa ttatggtaaa acatttacaa gaccatattc aatttttaga gcagtttata
    22381 aataacgtta acgcattaac tgcaaaaatg ttgaaagatt tacaaaatga atatgaaatt
    22441 tcattagagc agtctaacgt attaggtatg ttaaataaag aacctttgac aattagtgaa
    22501 atcacgcaaa gacaaggtgt aaataaggcc gcagtaagcc gacgaattaa aaagttaatc
    22561 gatgctaaat tagttaagtt agataaacca aatttaaata ttgatcaacg tttgaaattc
    22621 ataaccttaa ctgacaaagg tagagcatat ttgaaagaac gtaatgcgat tatgacagat
    22681 attgcgcaag atattactaa tgatttaaat tctgaagata ttgaaaatgt aaggcaagta
    22741 ttagaagtca tcaatcatcg cattaaaaca tatagcaatc ataaataggt ttttgaaggt
    22801 gttcaaatat aagact
//
LOCUS       DAHHRR010000032        22743 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.032, whole genome shotgun sequence.
ACCESSION   DAHHRR010000032 DAHHRR010000000
VERSION     DAHHRR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22743
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.032"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            181..1494
                     /gene="murT"
                     /locus_tag="NBI42_001077"
     CDS             181..1494
                     /gene="murT"
                     /locus_tag="NBI42_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HCD1777505.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKKGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNGDDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLHLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQAIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            1496..2227
                     /locus_tag="NBI42_001078"
     CDS             1496..2227
                     /locus_tag="NBI42_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCD1777506.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHNFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEKQAKQVLIDRANRQKKSR"
     gene            2313..2423
                     /locus_tag="NBI42_001079"
     CDS             2313..2423
                     /locus_tag="NBI42_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777507.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            2452..2865
                     /locus_tag="NBI42_001080"
     CDS             2452..2865
                     /locus_tag="NBI42_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777508.1"
                     /translation="MKPTKVILRDASYVHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMEYENPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVTKSDFTKFINELASLH"
     gene            complement(3054..4040)
                     /locus_tag="NBI42_001081"
     CDS             complement(3054..4040)
                     /locus_tag="NBI42_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD1777509.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            4568..5326
                     /gene="map"
                     /locus_tag="NBI42_001082"
     CDS             4568..5326
                     /gene="map"
                     /locus_tag="NBI42_001082"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCD1777510.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            5543..5929
                     /locus_tag="NBI42_001083"
     CDS             5543..5929
                     /locus_tag="NBI42_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777511.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            5944..6645
                     /locus_tag="NBI42_001084"
     CDS             5944..6645
                     /locus_tag="NBI42_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCD1777512.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            6642..7685
                     /locus_tag="NBI42_001085"
     CDS             6642..7685
                     /locus_tag="NBI42_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1777513.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            7675..8304
                     /gene="vraR"
                     /locus_tag="NBI42_001086"
     CDS             7675..8304
                     /gene="vraR"
                     /locus_tag="NBI42_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HCD1777514.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(8418..9635)
                     /locus_tag="NBI42_001087"
     CDS             complement(8418..9635)
                     /locus_tag="NBI42_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCD1777515.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKEDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIVFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEGTNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(9913..10188)
                     /locus_tag="NBI42_001088"
     CDS             complement(9913..10188)
                     /locus_tag="NBI42_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1777516.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVRDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(10195..10659)
                     /locus_tag="NBI42_001089"
     CDS             complement(10195..10659)
                     /locus_tag="NBI42_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCD1777517.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            10806..11006
                     /locus_tag="NBI42_001090"
     CDS             10806..11006
                     /locus_tag="NBI42_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HCD1777518.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHD"
     gene            11009..12265
                     /locus_tag="NBI42_001091"
     CDS             11009..12265
                     /locus_tag="NBI42_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCD1777519.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAKKGEAVLNDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            12355..12885
                     /locus_tag="NBI42_001092"
     CDS             12355..12885
                     /locus_tag="NBI42_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD1777520.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDDGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(13145..14296)
                     /gene="yfkAB"
                     /locus_tag="NBI42_001093"
     CDS             complement(13145..14296)
                     /gene="yfkAB"
                     /locus_tag="NBI42_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HCD1777521.1"
                     /translation="MNSVKLKQPVSIYNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHNRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRTAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            14520..14681
                     /locus_tag="NBI42_001094"
     CDS             14520..14681
                     /locus_tag="NBI42_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777522.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            14811..15326
                     /locus_tag="NBI42_001095"
     CDS             14811..15326
                     /locus_tag="NBI42_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCD1777523.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTEVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEEYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            15666..16475
                     /gene="sgtB"
                     /locus_tag="NBI42_001096"
     CDS             15666..16475
                     /gene="sgtB"
                     /locus_tag="NBI42_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCD1777524.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            16736..17554
                     /gene="recX"
                     /locus_tag="NBI42_001097"
     CDS             16736..17554
                     /gene="recX"
                     /locus_tag="NBI42_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCD1777525.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEMFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            17532..17846
                     /locus_tag="NBI42_001098"
     CDS             17532..17846
                     /locus_tag="NBI42_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HCD1777526.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            17861..19378
                     /locus_tag="NBI42_001099"
     CDS             17861..19378
                     /locus_tag="NBI42_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1777527.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVGKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            19387..20223
                     /locus_tag="NBI42_001100"
     CDS             19387..20223
                     /locus_tag="NBI42_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777528.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIALIVFAVLMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGICVLCFLVLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(20484..21461)
                     /locus_tag="NBI42_001101"
     CDS             complement(20484..21461)
                     /locus_tag="NBI42_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1777529.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADSSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            21613..22650
                     /gene="mutY"
                     /locus_tag="NBI42_001102"
     CDS             21613..22650
                     /gene="mutY"
                     /locus_tag="NBI42_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCD1777530.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPIQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARREMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGTINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYRFING"
BASE COUNT         8238 a         3196 c         3983 g         7326 t
ORIGIN      
        1 actctaattg agaaaatgta tcatttagcg agatttaatt gagaataaat atcattttta
       61 attatctaaa tgagaatgat tataatttag aaacgctgta tcaatttatg attgagaaac
      121 ttttttgaag ttgccttatc atgttataat gatgcgagtg gaaatcagag gaggaaacac
      181 atgagacagt ggacggcaat ccatctagcg aagttggcgc gtaaagcaag tagagcggta
      241 ggcaaaaaag gcactgattt accaggacaa atcgctagaa aagtggatac agatatatta
      301 agaaaattag cagagcaagt tgatgatatt gtatttatca gtggaacaaa tggtaaaaca
      361 acgacttcaa acttaattgg acatacttta aaagcaaata atattcaaat tatacacaat
      421 aatgaaggtg ctaatatggc tgcaggtata acttctgcat tcatcatgca atcaacacct
      481 aagactaaaa ttgcggtaat cgaaattgat gaaggttcga ttccacgtgt gttaaaagaa
      541 gttacacctt caatgatggt atttactaat ttctttagag atcaaatgga tcgcttcggt
      601 gaaattgata ttatggttaa caacattgca gagacaatta gtaataaagg catcaaatta
      661 ttgctaaatg gtgatgatcc atttgtgagt cgtttgaaaa tcgcaagtga tacgattgtg
      721 tactatggta tgaaagcaca tgcacatgaa tttgaacaaa gtacgatgaa tgaaagtaga
      781 tattgtccaa actgtggtcg cttattgcaa tacgattata ttcattataa tcaaattggt
      841 cattatcact gtcagtgtgg tttcaaacga gagcaagcaa aatatgaaat atcaagtttt
      901 gatgtggcac cgtttttaca tttaaatatc aatgatgaaa aatatgatat gaaaattgca
      961 ggtgacttta acgcttataa cgcgttagca gcatatacag ttttaagaga gctagggtta
     1021 aatgaacaag caattaaaaa tggctttgaa acgtatacat cagacaatgg tcgtatgcag
     1081 tactttaaaa aagaacgaaa agaagcgatg attaatttag ctaaaaaccc tgcaggaatg
     1141 aatgcaagtt tatcagttgg tgaacaatta gagggcgaaa aagtgtatgt tatttcgcta
     1201 aatgataacg ctgcagatgg tcgagatact tcatggattt atgatgcaga ttttgaaaaa
     1261 ttatctaagc aacaaattga agctatcatc gtgacaggta cacgagcaga agaacttcaa
     1321 ttgcgattga agttagcaga ggttgaagta ccaattatag ttgagcgtga tatttataaa
     1381 gcaacggcaa agactatgga ttataaaggt ttcacagttg caataccaaa ctatacatca
     1441 ttagcgccta tgcttgaaca attaaaccgt tcgtttgaag gaggtcaatc ataatatgca
     1501 tgaattgact atttatcatt ttatgtcaga taaattgaat ttatacagtg atataggaaa
     1561 tattattgct ttaagacaac gtgcaaaaaa acgaaatatt aaagttaatg tcgtagaaat
     1621 caatgaaaca gaaggtatta cctttgatga atgtgatatt ttctttatcg gtggtggaag
     1681 tgatagagaa caagcattag caacaaaaga attaagtaaa attaagacac cacttaaaga
     1741 agcgattgaa gatggtatgc cgggattaac gatttgtgga ggctatcaat ttttaggaaa
     1801 aaaatatatc acgcctgatg gtacagaatt agaagggtta ggtattttag atttttatac
     1861 tgaatcaaag acaaaccgat taacaggaga tattgttatt gaaagtgata cttttggaac
     1921 tattgtaggt tttgaaaatc acggtggtag aacatatcat aatttcggta cacttggtca
     1981 tgttactttt ggttatggta ataatgatga agataaaaaa gaaggcattc attataaaaa
     2041 tttattaggt acttatttac acggaccaat tttacctaaa aattacgaaa tcactgatta
     2101 tctgttagaa aaagcttgtg aacgtaaggg tattccgttt gagcctaaag aaatagataa
     2161 tgaagcggaa aagcaagcga aacaagtatt aatagacaga gcaaatagac agaagaaatc
     2221 tcgttaactc tgaacatcgc atcaatggat ttaaaattga taaacgatga agtatagtaa
     2281 ttaatcatat atgtataaac acacacatta ttttggatgg aaacaaccaa attgatgtgt
     2341 gtttttttgt tctagtgaat aattattata caatgagtat ctatcctaga attatcaata
     2401 gtaatggtga ttatgcaaca tgaaaaaatg aataatgaaa ggaatttgac gatgaagcct
     2461 actaaagtga ttttaagaga tgcatcttat gtacatagta aaacatcgat aacatttatt
     2521 ttaaaagatg tagttatcga agaagataat aaaatttatt atttcgacac tagtgcaact
     2581 ttcgaagatc aagaagttaa atttgaattt gcactttttg atagtgacat ggacaattta
     2641 aaacatatgg aatacgagaa tccagttacc gaaatatatt ttatagaacc tgatttacat
     2701 tttacgatta ttgattttaa tcaagaattg ctttgtattt atattgattt tgattctggt
     2761 ttaaggcatt caaacatggc aacagaatct ggtatttcat taaggataaa cgttactaaa
     2821 tcagatttta ctaaatttat taatgaatta gcctcgttac attaatgatt taaatctgat
     2881 acataattac aatcaaaaaa gacagccaca tccctccgta gtttaggtgt gtggctatat
     2941 ttgagtctga atatttacga ttgtaatttt aaaaagggac atgctatata cgataaaatt
     3001 acttataaag taagaatata tctaatggtg ccaattagca taaggtactt aaattaagca
     3061 tatctgctgt ctagcagtcg ataaatcatt agaacttcat acagtatatg acttttaatt
     3121 tgattttcac cactaatttc aagtgctttt attgtcgaac gtaaagtttc tacagaatca
     3181 tcttctctct taaatgaacc atcataaaaa atatctttga tgctactact aatttttagt
     3241 aatgccattt tttcgtcacc tgaaaagtta acacgagtat ttttaggcaa gtaaatgata
     3301 tttgataaat gagtgataaa caaacgattc gtatatgcac gtttagttaa ttgattgagt
     3361 aatttccaat cacattcttt tttcttatga tagcttaatt catcacgttg ataacttatt
     3421 aacgtttcaa cttgattgtt taaattgaaa atatttttat atgctttttc gcttttatca
     3481 gattgcagtc ttgataagat aagttcttgg cagcgattgt aaaataattt atacatcaag
     3541 gcatctgtct tacttaattt ttcttcgacc tgaccataat acttaggtgg aaacaccatg
     3601 aagttaatta aacctgatgt cacgagtcca ataatcgctg tcaatgttcg agacaaaaag
     3661 ttgaatatgt aggcatcatg aatacctgga atcatagcta atgatgttag tacagcaaca
     3721 ttcgtaccaa cttgcaattt gagttttgta cagaataaaa tcgtgaacgt cgcactcaat
     3781 gcatatgtaa aaggtgattg atcgccgaat aaatatgtaa ataatactgc aaagcctgca
     3841 ccaattaccg tagcaggtaa tctacgataa cctttaataa gtgatgcctt ggcagttggt
     3901 tcaattgtga ctacagctgt taaaatggca tagataggtg ttaaatctag tgccatacaa
     3961 aagacagctg ttaaaaaaat ggcaatacca gttttaattg ttctggcacc aattaaatgt
     4021 ttataccatt gatcgttcat tttttaacct ctaatcatcg taaaatctta gcgagcgctt
     4081 tataataata gtatcgtaca ttggaaaagt tcatgtatgt aaaatatttg aaataatcat
     4141 gcttaagcat tactttgatt ttcatataca ttaatcaaag ttctgggctc cacaatgtta
     4201 tattgttagt agttgactga aataaaatgc acttgtaaca agcatttttc aattctagtc
     4261 cggggcccca acacagaagc tgacgaaaag tcagcttacg ataatgtgca agttggggtg
     4321 tgggccccaa cacagagaat ttcgaaaaga aattctacag gcaatgcaag ttggggaggg
     4381 gccccaacac agaagctgac gaaaagtcag cttacgataa tgtgcaggtt ggggacaata
     4441 aagaaatatt ttttctttat cacactatgt cccactcact ttccaaaata ctaaagtaac
     4501 atctttagta tatcaaagaa tttttgctat aataagttat aattatataa aaaaggaacg
     4561 ggataaaatg attgtaaaaa cagaagaaga attacaagcg ttaaaagaaa ttggatacat
     4621 atgcgctaaa gtgcgcaata caatgcaagc tgcaaccaaa ccaggtatca ctacgaaaga
     4681 gcttgataat attgcgaaag agttatttga agaatacggt gctatttctg cgccaattca
     4741 tgatgaaaat tttcctggtc aaacgtgtat tagtgttaat gaagaggtgg cacatgggat
     4801 tccaagtaag cgtgtcattc gtgaaggaga tttagtaaat attgatgtat cggcattgaa
     4861 gaatggctat tatgcagata caggcatttc atttgtcgtt ggagaatcag acgatccaat
     4921 gaaacaaaaa gtatgtgacg tagcaacgat ggcatttgag aatgcaattg caaaagtaaa
     4981 accgggtact aagttaagta acattggtaa agcggtgcat aatacagcta gacaaaatga
     5041 tttgaaagtc attaaaaact taacaggtca tggtgttggt ttatcattac atgaagcgcc
     5101 agcacatgta cttaattact ttgatccaaa agataaaaca ttattaactg aaggtatggt
     5161 attagctatt gaaccgttta tctcatcaaa tgcatcattt gttacagaag gtaaaaatga
     5221 atgggctttt gaaacgagcg ataaaagttt tgtcgctcaa attgagcata cggttatcgt
     5281 gactaaggat ggtccgattt taacgacaaa gattgaagaa gaatagttca acatatacta
     5341 agactaaagt atgaacatca tttagttccg gagcctattc atattggttt cggaactgtt
     5401 ttataataat taagaacaca atcaatgcgt catttcaaaa atatgttgta ataaagtagt
     5461 ttttaagcaa acatatcatc gacatcaacg aagatacata gcgcatttgg tattttaaaa
     5521 cttattataa aaggtgatag ttatgaacta tgttgaacgt tatattgaac agtttttgag
     5581 agcaacagta agaaataata tcaagcacta ccttttaatg ttagatgaaa aaatgaaaaa
     5641 tttagatgat tatatgcgtt atttaattac taaaaaagaa caacttagca agttaattga
     5701 cagtctaatg ctaacattag aaaataaata tattgatatt gctgaagcat ttcaaattca
     5761 atgtgcaaga gaaatcaata atcaagaaat tgaaaatatt aaatcagagt tgaataaagt
     5821 tgaagcatat tatgcacaaa ttgaaactca aattcaacaa acttcaactg aaaaaatagc
     5881 aacagaaaaa acatcgtatc taataaatta tatgaacgct gtggcataga aaggcggcga
     5941 aacatgacac acaaatatat atcaacgcaa atgttgatca tttttactgc attaatgatt
     6001 attgccaatt tttactacat attttttgaa aaaattggct ttttactcgt tctattattg
     6061 ggatgtgtat tagtttatgt aggatatctt tattttcata aaatacgtgg ccttttggcg
     6121 ttttggatag gcgcgctatt aattgcattc acattattgt ctaataaata tacaatcatc
     6181 atcttgttcg tctttttatt actacttatt gtgcgttatt taatacacaa gtttaaacca
     6241 aaaaaagtag ttgcgacgga tgaggttatg acttcaccat cttttattaa acaaaagtgg
     6301 tttggtgagc aacgtacacc agtttatgta tataagtggg aagatgtaca aattcaacat
     6361 ggaattggcg acctacatat tgacttaaca aaagctgcaa atattaagga aaataatacc
     6421 attgttgtta gacacatttt aggtaaagtg caggttatat tgccggttaa ttacaatatt
     6481 aatttacatg tagctgcttt ttatggaagt acttatgtga atgaaaaatc atataaagtt
     6541 gaaaataaca atattcatat tgaagaaatg atgaaaccgg ataactatac agttaatatc
     6601 tacgtatcaa cgtttatcgg agacgtagag gtgatttatc gatgaaccac tacattagaa
     6661 caattggttc aatgctcatc ttagtatata gcatgctagc tgcatttctg ttcatcgata
     6721 aagtttttgt aaatatcatc tattttcaag gtatgtttta tacacaaata ttcggaatac
     6781 cagtcttttt atttttaaat ctcatcatca tattattgtg tattattgtt ggttcggtac
     6841 tcgcttacaa aatcaatcag caaaatgatt ggattaagac gcaaattgag cgttcaatgg
     6901 aaggcgaaac agttggcatt aatgatcaaa atatagaaat atatagtgaa acgttagatt
     6961 tataccatac actcgtacct ttaaatcaag aattgcataa gttgcgactt aaaactcaaa
     7021 acttaaccaa tgaaaattat aatattaatg atgtgaaagt taaaaagatt attgaagatg
     7081 aacgtcaacg actagcacga gaacttcacg attctgttag tcagcaactt tttgcggcaa
     7141 gtatgatgct atctgctatc aaagaaacga agttagaacc accattagac caacaaattc
     7201 cgattttaga gaaaatggtt caagattcgc agttagaaat gcgtgctttg ctgttacatt
     7261 taagaccgct tggtttaaaa gacaaatctt taggtgaggg tattaaagat ttagttattg
     7321 atttacaaaa aaaagtgcca atgaaagttg tgcatgaaat acaagatttt aaagtgccta
     7381 aaggtattga agatcatttg ttcagaatta cacaggaagc aatttcgaat acattgcgtc
     7441 attcaaacgg tacaaaagtg acagtagaat tgtttaataa agacgattat ttattgttga
     7501 gaattcaaga caatggtaaa ggttttaatg ttgatgaaaa attagaacaa agttatggac
     7561 ttaaaaatat gcgtgaaaga gctttggaaa ttggtgcaac gttccatatt gtatcattgc
     7621 cagattcagg tacacgtatc gaggtgaaag cacctttaaa taaggaggat tcgtatgacg
     7681 attaaagtat tgtttgtgga tgatcatgaa atggtacgta taggaatttc aagttatcta
     7741 tcaacgcaaa gtgatattga agtagttggt gaaggcgctt ctggtaaaga agcaattgcc
     7801 aaagcccatg agttgaagcc agatttaatt ttaatggatt tacttatgga agacatggat
     7861 ggtgtagaag cgacgactca gattaaaaaa gatttaccgc aaattaaagt attaatgtta
     7921 actagtttta ttgaagataa agaggtatat cgtgcattag atgcaggtgt cgatagttac
     7981 attttaaaaa caacaagtgc aaaagatatc gcagatgcag ttcgtaaaac ttctagagga
     8041 gaatctgttt ttgaaccgga agttttagtg aaaatgcgta atcgtatgaa aaagcgcgca
     8101 gagttatatg aaatgcttac agaacgagaa atggaaatat tattattgat tgcgaaaggt
     8161 tactcaaatc aagaaattgc tagtgcatcg catattacta ttaaaacggt taagacacat
     8221 gtgagtaaca ttttaagtaa gttagaagtg caagatagaa cacaagctgt tatctatgca
     8281 ttccaacata atttaattca atagttcata tcgaattaag aaaagttact taagccaatc
     8341 acaatataac atcaaatata caccaaaaaa acagaggtct tcgcaatcat tagcgaaaac
     8401 ctctgttttt ttggtgttta gtctttttta agcttatcaa cgattttgtc ttttatattt
     8461 ttatcatctt ctggataggt atcactagga tgttcatcaa catgatacgt acgttgatgt
     8521 gttgcattag taccttcatc atattcgtac ctagtgttct cattataatg atttttatta
     8581 ttgtcatcat gttctaatgc tgcttcttca ggtgttttac ctttaattac actacgctga
     8641 tgaatgattg cattgatttc agcaccgaca attataataa aacttgtgat atataaccat
     8701 agtaacaaaa tgatgatacc cgcgatactg ccatatgttt tagaatagtt accaaaattt
     8761 gaaatatacc aaccaaaacc aaatgaacca gctaaccaaa taattgaagt aaatactgca
     8821 cctggtaata ctgacttaag cttcgtttta acgttaggtg caaccgaata taacacgata
     8881 aatacgataa atataataat gattggtaac acaattctaa taagattaaa aatccatttc
     8941 acttgttcgt caaatccaag tggaccgaat aggaaatgac taattacaga accaagcgtt
     9001 ggtaatgcta gagcaactac aaacacaacg cccataacta cagtgaagac aacacttagt
     9061 agttttaata cgattccatt acggctatct tctacatcgt aagcaacatt gaaagaattc
     9121 ataattgcag tcattccatt tgaagctgac caaattgcta aaatcaaacc gatagataat
     9181 aagccaccac tggagttttg agttatatca ccaattacac tcttaattag agtggatgtt
     9241 tcagcgggtg cattacttaa catattagta atttgactct gcttaatatt gaaaaatggt
     9301 aatagtgtta ataggaaaag caacataggg aacatagcaa gtacgaaatg gtaagtcatt
     9361 tgcgctgcta gtcctgaggc atcatctttt cctatgcgat agatgagata tgataagaaa
     9421 ttagagtctt tcttatattt tgcaggttta tttaatcttg ataagaagaa aacctgatcc
     9481 tcttttttag gttttttaga ttggaattgt tgaggttcaa tatatgtacg atcaacatca
     9541 attttgggat tacttttatt tttctttttt gactcctctt gcgcttcctt aacagaatta
     9601 aggtattttg aagaagagtg atcctttttc gacatgtgca ctccttaacc tttctctatg
     9661 taatgtttta taaagtaact attagtattt atccaattat aaaaaaacta aacaatgata
     9721 tgctttaaaa atgataatat tatagaaatt aattgattta aatctttcat aggctatttt
     9781 gatttaaaat taaaacaaag gcaatactga aacaatgcag tttggccaat cattgtatta
     9841 tcatttaata atgataacta tgaaaggcgt acaccaaatt gttaagtatt gccttataaa
     9901 aacaaaatca tcttagcgtt gattttttct attaacaaat gtttctttcg catcctttaa
     9961 tgaacgttct aattcaggat tattacgacg aatttcttca acaacatctt tccagtataa
    10021 aacttcgtct ttaatcttgc tgactttaga aggcttacga gtgcggttac cattttttgc
    10081 atctctaact gattgaacta aagcttgacg tgtagattta tcagctaaac taattgcgcc
    10141 accaattacg gcaccaatta aaataccagg aacaaattta ttttccataa aaaactaccc
    10201 ctctttcaaa tttgcatctt ttacgatgta gtctattaaa ttatcacaag atgataatac
    10261 catgtcgtat acaccttcaa aattattcgt gtagtatgga tctggtacat cactctcttc
    10321 catattacta aattctaaca gtttgaacaa ttgtccctta agattaggat tgatagattt
    10381 aatattatca acgttacttt gatccatagc cacaatgtaa tcaaaatcat ctgtcgcttc
    10441 gaataattca ctaatcatgc catcaaatgg aatattgtgt ttgttaagaa ttttttgtgt
    10501 accttcatga ggcggctctc ctaaattcca gctaccagta cctcttgaat gtacttttat
    10561 atcatgaata tttctgtctt taagtctttg tcgcatgatt gcttctgcca ttggagaacg
    10621 acatatattg ccaagacaga caaatgctac atctaccatt ttgattcctc caaactatgt
    10681 agttatatcc ccattttata gcgactttaa acaataagaa agcagattat ataaaattct
    10741 attaaagttt attaaattgt gatactttga taacataact attattagag gtgaacattg
    10801 tggctatgac aaatgaagag aaagtattag ctattagaga gaagttaaat attgttaatc
    10861 aaggattact agatcctgaa aaatataaaa atgcaaatga agaagaatta acagatatat
    10921 atgattttgt tcaatcaaga gaaagattgt cgccaagtga agtgacagct attgctgacg
    10981 ctttaggaca attgcgacac gactaggagt gggaattttg actaattaca aagaaaagtt
    11041 acaacaatac gctgaactat tagttaaggt tggtatgaat gttcaaccaa aacagccagt
    11101 atttattaga tcatcagtag aaacattaga attaacgcat ttaattgtag aagaagcata
    11161 tcattgtggc gcttcagatg tacgtgttgt atatagcgat ccaaccttaa aacgcttaaa
    11221 atttgaaaat gaatctgtgg aacattttgc taatcatgag ataaaatcat atgatgttga
    11281 agcgagaatg gattacgtta aacgtggcgc tgctaattta gcacttatta gtgaggatcc
    11341 tgatttaatg gatggtatcg atagccaaaa attacaagca tttcaacaac aaaacgcacg
    11401 cgcatttaaa ggatatatgg aaagtgtaca aaaaaatcaa tttccgtggg tcgtagctgc
    11461 ttttccatct aaagcttggg cgaagagagt atatcctgaa ctatctgtag aagaagcata
    11521 tattaagttt attgacgagg tattcgatat tgtgcgaatt gatggtaacg atccagttga
    11581 aaattggcgt caacacatcg ctaatttaag tgtttacgct caaaagttac aacaaaaaaa
    11641 ttaccatgcg ttgcattatg tatctgaagg tactgattta acagtaggtt tagcgaaaaa
    11701 tcatatctgg gaagatgcta caagttatgt taacggcaag gaacaagctt ttattgctaa
    11761 tataccaact gaagaagtat ttacagcacc agatcgaaat agagtagatg gatatgttac
    11821 taataaattg cctttaagtt ataatggtac aattatcgat caatttaaat tgatgtttaa
    11881 agatggagaa attattgatt tttcagctaa aaaaggtgaa gcggtattga atgatttaat
    11941 caataccgat gaaggttcaa gaagattagg tgaagtagcg ttagtacctg atgattcacc
    12001 gatatcaaat cgtaatacca ttttttacaa tactttattt gatgaaaatg cagcatgtca
    12061 tttagcgatt ggatctgctt acgccttcaa tattcaaggt ggaacggaaa tgactgttga
    12121 ggaaaagatt gcaagcggat taaatgattc aaacgtacat gtcgacttca tgattggtag
    12181 tagtgatttg actatttatg gcatattcga agatggttca aaagaactag tatttgaaaa
    12241 tggaaattgg gcatcaacat tttaataaat gttattttga ggtgctgagt aggaaatgaa
    12301 acatgtattt aaaggtcaaa tacgtgatta aagtatagat tgggagataa aataatgaca
    12361 aatcaggaca gaccaatgaa atctatgtca gaatcaaaat gttataaaaa tagacaagtt
    12421 ttccctcaag atacgaatca ccatcataca atgtttggtg gtacattgat ggctaatatt
    12481 gatgaaattg cagcaatcac agctatgaaa catgctggtg cgcaagtagt tactgcatct
    12541 acagactcag tagatttctt aaagccgatt aaaacagggg acatattaca atacgtagcc
    12601 atggtttcat acgctgggac aagttcgatg gaagtggtcg ttcaaattag aattgacgat
    12661 gtatttaata acaagcatga tttagcggca ttaagttact taacatttgt tgcgttagat
    12721 gatgatggga agcctaaaca tgtacctgga gtatatccag aagatgatgt tgaaaaatgg
    12781 ttttatgata cagcaccaca acgtgttgaa cgaagaaaag cacgtcgcat tgaaagtaaa
    12841 caaacaattg aatatttagc tcaagtgcaa catattagag actaaaaata taaagacaga
    12901 ttgttaagga tgaattgaac tgttatgttc gaatattcca ccaacaatct gtctttttga
    12961 ttatgcacat ttatgttttc agttaaaatt taaccctttt attatagact attagtccta
    13021 ttaaatgacc tttattattt aagtagagtg ctgccagcga ttaattgttt gtaattttgt
    13081 aatagcatta aaatatgcgg agactactta tcaataaagt gcttttccgc ataattaagc
    13141 gtttttaaaa ttgtataatt tgtggttgtt tgtgcatatg acgctcatta tctttaaaat
    13201 ccatattcgg atagtacata tttttaacaa gaacatttgg tcctaaacaa ctaaactcag
    13261 aacaatgaca atttaatgat ttagcaagat cagaggataa ccatttatca aatacatctg
    13321 ttaatttatc tttttgtata ttcgaaattg tacctgtttc atctccgaaa tcagttacga
    13381 ttacattacc tgtaaataca ttgacattta aacgactacg gccatccggg tcatttctag
    13441 tcgttacatt tttagctgtt cttaaacgtg atagtaactt ttgatcttca tcatctttta
    13501 agcatggaaa cactggcaaa gtaccaaata acatccaaat atcttcatct ctgaaatcca
    13561 atatatcatg aattgtcttt ttcatttccg ctagagttaa cacatttaat tgacttgcaa
    13621 agtcagctgg atacataggg tgaatctcgt gtctgctaca tttcatatca tgaacgactt
    13681 cttgatgtat ttttcgtaaa tgtggtagcg tactttgatt gagcattgtt tccgcagata
    13741 caaacattcc ttgttctgat aatgtacgtg cattcgaaat catttgttca tataatttta
    13801 acttagcttt taacggtggt tgcttcttca ttgcgccaaa gccaacattt gcgaattcat
    13861 cagttgttcc ccagttatgt gagatatgca taacatcgat atattcagca atatctaaat
    13921 aacgatcttg aggcaatgtt aggtttgaat tcatttgtgt atatatacct cgattatgtg
    13981 catactttaa tagaggttta acaacatttc taatagactt tttagaaaac attggttcgc
    14041 cacctgtaat tgacatcgtt cgcagatttg gaatttcatc aagtctacga taaattaagt
    14101 ccatatctaa aggctcgggg tcgacagttt gtaaagtata accaactgca cagtggctac
    14161 aacgcatatt acaaagattt gtagttgtaa attcgatgtt acttaaagtt aattggccat
    14221 gttctttaac atcgttatat gcctcccatg gatcattgta aatgctaaca ggttgcttca
    14281 atttgacgct attcatcgtc ttaaacttcc ttctttctat ttggttcata tcggtatgat
    14341 accacttcgt attatcactc gtaacaaatt atctcttaat tttagttaat tgaaaagggg
    14401 atttttaaaa gtcaaaataa tttgaatgtc ataaatatag ctaagattat atcgctttaa
    14461 cgcaatttct gttaggataa taatgaaaaa gaaaacaaaa taaaaggagt taacttttta
    14521 tgaatgaaca gcaaacaatc gaacagataa aagcgcgttt aaataagttt attgaggata
    14581 tcgatcatgt aaatcctgat gaagtacgtg ttgaagatat agatgaatgg attggattgt
    14641 tagatcagct tgaagaaaag gttaaattag tatctaagta aataccacat aagatgtgtt
    14701 agagttagat atcgcgacag tcgcttaata tttttatatc ttgtatgttt aatcattgat
    14761 ttacttgtct atagtattta tatcaatgat atatagaaag gtgattttca atgactaaaa
    14821 aagtagcaat tattctagca aacgaatttg aagatataga atattcaagt cctaaagagg
    14881 cattagagaa tgctggcttt aatactgaag tgattggaga tactgcaaat agtgaagttg
    14941 ttggtaaaca cggtgaaaaa gttactgtcg atgtaggtat tgcagaagct aaaccagaag
    15001 aatatgatgc attattaatt cctggaggat tttcaccaga tcatttacgt ggagatacag
    15061 aaggtcgata tggcacattt gctaaatact ttactaaaaa tgatgtacca acatttgcca
    15121 tttgtcatgg gccacaaata ctaatagata cagacgattt aaaaggtcgt acgttaacag
    15181 cagtattaaa tgtacgtaaa gatttatcaa atgcaggcgc acatgtagtt gatgagtcag
    15241 tagttgtaga caacaatatt gtaacaagtc gagtaccaga cgatttagat gattttaatc
    15301 gagaaatcgt taagcaatta caatagagta ttgcggttat caattgttaa aatttatata
    15361 ttttcgtttg aaatgaaata tggattttag ttttaaaaaa tgaataacaa ataaaaggtc
    15421 gtctctattg gcatttaata gggatggtct tttatttttg taaaatataa caatatttga
    15481 catgattaat atttaaaacc gcaatgattt atctataatc ttttcaaaaa acaaaaaagt
    15541 aagtatgatg gcgtatacga ttgtaagccc ttggactgat tttcccaaaa agtgtagcct
    15601 atttgtgata gtactggtaa actcaaggta tatactaagt gagttttaaa agaaggagca
    15661 aacgcatgaa aagaagcgat aggtactcaa actcaaatga acattttgag catatgaaac
    15721 acgaacctca ctataatacg tattatcaac cagttggcaa accgccgaaa aagaaaaaaa
    15781 gtaaacgaat actattgaaa atattattaa ccattctaat cattatcgca ttgtttattg
    15841 gtatcatgta ttttttatct acacgcgata atgtggatga actaagaaaa atagaaaata
    15901 aaagtagttt tgtgtcagct gataacatgc cagagtatgt taaaggtgcc tttatttcaa
    15961 tggaagatga acgattctac aatcatcatg gatttgattt gaaaggtaca actagagctt
    16021 tattttcaac gattagcgac agagatgttc aaggtggtag taccattaca caacaagttg
    16081 tcaaaaatta tttttatgat aatgatcgtt catttactag aaaagtaaaa gaattatttg
    16141 tagctcatcg agttgaaaaa caatataata agaacgaaat tttaagcttt tatttaaata
    16201 atatttactt tggggataat caatatacgc ttgagggtgc agcaaaccat tactttggaa
    16261 caaccgtgaa taaaaatagt acaacaatgt ctcacattac agttttacaa agtgctattt
    16321 tagctagtaa agtcaatgca cctagcgtat ataatatcaa taatatgtct gagaatttta
    16381 cgcaacgtgt aagcacgaac ttagaaaaaa tgaagcaaca aaattatatc aatgaaacac
    16441 aatatcaaca ggctatgtca caattaaatc gttaaatact tgcatttatg tgtaagatac
    16501 ttggaacaat aatgtagaag gtttaattga aatgtcataa tgataaagtt gaattgtttc
    16561 tgattaataa attttacaga tttgcttgta atcatacttt cattttaaaa gatgatagtg
    16621 tgattttttg ttagcatcga aaaatattta aagttaaaca tttgctatag gaagtggctt
    16681 attgaataat aaaagagtat tgtattaaaa cttgagaaag taacggggtg atccagtgcc
    16741 gaagattact aaaatagaag ttcaaaagaa aaacaaagaa cgttttaatc tttttttaga
    16801 cgaacaattt gaaatgggta tagatatcga tacattagtc aaatttaatt taaaaaaagg
    16861 gcaacaactt gaagctgctg atatggcaga gattcaaaag tatgatcatt atcgcatagg
    16921 tttaaataaa gcaatccaat atttatcata taaaaagaga actgaaaaag aagttataca
    16981 atatttacaa aaagaagaga tatcagagca agcgatttct gaagtgattg aatattgtta
    17041 tcgcgaaaag ttaatcgacc atcaagatta tgcggaaagt ttaaaaaata caatgattcg
    17101 cacgacagat aaaggaccta aaatttatca acaaaaacta tatcaacttg gtatagaacc
    17161 aaatatcatt gaaatgttta cagaacttta tagagaacaa caggaacttg atgatattat
    17221 ccaaattgct gaaaaaatat ctaaaacaaa aaaagggcca caaaataagg ttaaagaaaa
    17281 agtaatgcaa tctttaattc aaaaagggtt tgaaatggaa accattcatg ctgtattgaa
    17341 tgaaatggat tttacacaag atgaagcggt tttagacgat ttattacaac gagatttaga
    17401 aaaaatttat aataaaaatc gaaagaagta cacgcaacag aaattaattt caaaaactat
    17461 cgaaggcctt atgagaaaag gatataaata tgataaaatt aaagctaaat tagaagaaag
    17521 tggtattgcc gatggaacag aagaaattga gtgaaatgtc tgagccagaa ttaagacacg
    17581 aaatacaact ttataaagag aaaatgagaa aagctgagat gaatggtatt ttaaatgaat
    17641 atgacgttta tcaaagtaaa gtaattgttg ccgaaagtta cttagtagac agaaaaaaga
    17701 tagaaattgg taaaatttat aaactgactg atggcagtaa tcaatatttt aaagtagaaa
    17761 gattaaaagg tatttttgcg tgggggttta gatttaatag cgatgaaccc gaagaaggct
    17821 tacctatagc gttattacaa ttatagaagg atgaaaatag atgggaagtt caattgtttt
    17881 aaagttacta aaagtaacac actactatag gaataaacag aataaaaaat ggtatttacc
    17941 ttttggatat gatgctgaag atatcgattt aaacaatatt agtttacata tttatcaagg
    18001 agaagcatta ggtataattg gtgaacctga atcttccaaa gcgttggtag gtcaattgtt
    18061 ggcaggtgca attaaacctg ataaaggtaa agttgtttgt actgaagatt tgttctacgg
    18121 atatattgaa gatcaatcgt taattcatca aactgttgaa gcttacacag cgcagttagt
    18181 tcaactattt ccatatgaaa ttaatgatca taaagctgaa cagattattc aatatgcaca
    18241 tttaggcgat tataaaacga agccggttaa ccatatttcg aaagcggcat acgctcaatt
    18301 actattaagt attgcacgct catcaaaatc aaatattatt attttaaatc atgttattga
    18361 ctatttaaca ccacaattta tggaacgtgc gattgaatta acaaatgatt atattgaaaa
    18421 taatttaacg attgtgtcaa ttggtgatga tattgataaa atttcgcaag tgagtaacta
    18481 tatagcttgg ttttcacatg gtcaattaag aatggaaggg tcacttaaac aagttattcc
    18541 atcttttaaa gaacatgaac gtgatcgatt aagtctaaac tcaaaagaag aaattgaaaa
    18601 ctttgattta gattggaaaa agaatcgtac aagaataccg gagatgacct ataatttcaa
    18661 acgcgttgag cgctataatc atgcaaaacc gcctaagttt ttagtgcgtt tttggacttt
    18721 agcctcaggt actattttag gcttagcatt gatgatgttg ctcattttca ataatatagg
    18781 aattatttcg ataacagatt ttacgaatcg tgctacgatg caaaatgaaa ataaagatcc
    18841 atatggcgaa aagttagctt atggaattgc ttttaatggc agtgtggata tgcaagggga
    18901 taaacaagtc acaattccaa aatatagtgt agttacaatt actggcgaaa atagtaaaaa
    18961 ttatcgtgtt accgccgata ataagactta ctatgttggt aaagataaat tagaatattt
    19021 taacccggca ggtttatatc aaacgcatag ttttaaaaaa ttagcaccat atatgaaatc
    19081 aaattatagt aattactatg catactttaa cagtcaatta cataaaaagc atagttcagt
    19141 tattaaaact ttagttcctg atgatgataa ccgtttcgtt gcatccgtta cacaacaacc
    19201 gatacaatta cttttcaatg ataataatca gttatacggt tttgtttatc caattgtaga
    19261 taaaaaagaa ttaaaagata agtttaatat taacaataac atttggatta ctaaagttgg
    19321 gaatggatat tgtattgcca atttgaaaga agacaaatgg atttatattg aattgtaggt
    19381 gtaaagatgc tagataatat tattttatat tttaaaaact taccgcatac taaaaggtat
    19441 gtaacagaac gtttaaaaca aagttggaaa agttttctaa tcgtattagc tgcatgttta
    19501 atacttatta ttgcaagtga aacgctattt tcctttagtc atttgaccga tgttaaagag
    19561 gtgcgatggt tatttagaat tattgcattg attgtttttg cggtattgat gttcacaatt
    19621 tatatctctt atcatcatta tatgaatgac tttttagtta ctaagttatt taatatttcc
    19681 gcagcgacgc cagtagttat tatgtctatt ttaagtttta ttatgctagt cattttgacg
    19741 atgattagtg cattggttaa accagtaaac ttcgaaacat catatatagc gctattctat
    19801 tttattgtga tggcaactat ttttgtagga ttaatatcag ttacttttgg tcttattcga
    19861 ctgttaactg aaaaaataaa tatcatattt tatgggattt gtgtattgtg tttcttagtg
    19921 ttaccaatta ttttcatacc aaatccaaat catgtattta tcaatcacat cctaatgctg
    19981 aatccaatgt attacattgt taatggtata gcacaatcta tcatatttgg tatatcaagt
    20041 atggaaaaca ttccatatca tttttacttt attttattct tatgtttaat agctgcagta
    20101 aatttcgtat tagcaaggta tacgacacac gcaatttata ataaaacatc taaagtgaca
    20161 caaactgata atcaacaaga tgtttcaaat gatagtacag acgaggcgga tacttcttca
    20221 tgatgtttag ctgacctgtt gtgtaaatta atctgtaaga atcagaaaca attagaatat
    20281 aatctagata ttgttttcct atactgattt tgatatttcg agtcattaaa ggtagtttgt
    20341 tatcaacaat ataaatttta ggttttgtaa aagcatttat agttttacat taaaaaaccg
    20401 gacagagtaa aaatcgtttt gattttcatg ctgcccggtt gtttattttt tatagattaa
    20461 ctttagtgaa taataacttg aaattattta gcatacagtt tacgttgtaa cttatcttct
    20521 gtaaataccc agccaatata agagtggtct atgacattat cgtttgttac atgtgcaatt
    20581 gccacaaatg aataatgatc attttttaaa taacgcaaat caatcaaacg aatttcagta
    20641 gaactatctg ctaacgttgt tgtttgccaa cgatagattg atgaaaagtt taaaaaagta
    20701 cgtatatctt tattaccttt gactttccat aaaatggagt ctgttgataa tgtttgacgt
    20761 tccactttat cactaaacac cacattccta ccatatgctt ttccaacata gtcatgtgca
    20821 tcagtttgta tcgcgacacg ccattccata aattttattg ttggcgcaac aaatacttta
    20881 acagggtgat gctcttgttg aattgctttt aaagcttgtt gcttaattac ggctctcatt
    20941 ttaaaacgaa tcatgtaata aatgattagt aaagcgatta taggaaagaa gactgcaaat
    21001 ggatgcaagc ctataaccca taatacaata ccaatacaaa gaactgtgaa aataataggg
    21061 tcaaatgtgt taatcacact taattgaatc catttgtttg tgataggtct aagtgcttgt
    21121 gtaccataag aattgaatat atctacaaag acatgtaaaa atactgcgag ctgagcccac
    21181 atccatacat gaaatgggtt ggttccacta aagaacgtga atattaaaaa tgtaattaaa
    21241 attggccata gtattgtgaa agggatggaa tgcgtgatac ctctatgatg cgaaatatat
    21301 gttgcattgt cctttaattt aagaacagta tccccatcag gaattaatga accaacgata
    21361 agggttgtag ctgttgcacc gaacgtagaa gccattgctg gatcttgagt tgcaagtgct
    21421 gtaaggccca ccccaattgc gatatgtgtt gcagtgtcca tatcagttca ctcatttcat
    21481 aatattatat atactattat aatccaattt cgcaaataac taaattaact tacatactaa
    21541 ccatgatttt aatgtacaat aaaaacatta tgagtaaaga caattaattt tagtgaggaa
    21601 agggttttat aaatgtatca gcagtctagt tttaaagaaa atttgataca ctggtttgat
    21661 gaaaatcaaa gagaaatgcc atggcgacaa acgaccaatc cgtactatat ttggttaagt
    21721 gaagttatgt tacaacagac acaagttaaa acagttattg attattatca tcgttttgtt
    21781 gaacgatttc caactgtgga agttttgagt caagcttcgg aagatgaagt gttaaagtac
    21841 tgggaaggct taggatatta tagtagagct cgaaattttc atacggcaat taaagaagtt
    21901 catgataaat atgaaggctt agttcccaaa gatcctgatc aatttaaagc gttaaaaggt
    21961 gttggcccat acacacaagc tgcagtaatg agcattgcat ataatgtacc actagcaact
    22021 gtagacggaa atgtttttcg ggtatggtct agattgaatg atgactatcg agatattaaa
    22081 ttacaatcga cgaggaaatc gtacgaacaa gaattattac cgtatgttac tacagaagct
    22141 ggaacgttta atcaagctat gatggaatta ggggcattaa tttgtacacc gaaaaatcca
    22201 ttatgcttat tttgtccaat tcaagaaaac tgtgaggcgt ttgataaagg tacatttgaa
    22261 aagttacctg ttaaatctaa aaatgttagt aaaaaagtaa ttgagcaatc cgtcttttta
    22321 attagaaata atcaaggtca atatctgttg caaaagcgta gcgaaaaatt attgcatggt
    22381 atgtggcaat ttcctatgtt tgaaagtgag catgctagac gtgaaatgac cgaaaaaatc
    22441 ggtcatgaca ttcaaccagt agaaacacct atttttgagt tgaagcatca atttactcat
    22501 ttaacatgga agataaaggt atacgccgtt tcaggaacaa ttaacattga aacattacca
    22561 gatgacatga tttggtttga tttaagtgat cgagatcaat atacattccc tgtacctatg
    22621 tcaaagattt atcgatttat aaatgggtaa cattcgatga ttgataagat tatcgaaaaa
    22681 tatagtgaca ttaattttaa aaatgtataa atgatatctt taaatctcat agaataacaa
    22741 tgt
//
LOCUS       DAHHRR010000033        22052 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.033, whole genome shotgun sequence.
ACCESSION   DAHHRR010000033 DAHHRR010000000
VERSION     DAHHRR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22052
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.033"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            463..1167
                     /locus_tag="NBI42_001103"
     CDS             463..1167
                     /locus_tag="NBI42_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD1777531.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNLKVIYMPHWLEFYS
                     IAIHSDVTNNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNHLKLNESVLNLTATDLKKQIIDISSKVQALAQKYRDIDIKISTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DINEIEKQLKIKFFSN"
     gene            1559..2095
                     /locus_tag="NBI42_001104"
     CDS             1559..2095
                     /locus_tag="NBI42_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777532.1"
                     /translation="MHNKNEFNKKLKDFIGSDKRMALVKGYINEYKLTTVLRALNNSD
                     YTKGTIHTRSIDNLKQIVDNRIIPKNITQNTIFKLSNLIIEVNLYERNTIYHGDFAIY
                     YLVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(2208..3749)
                     /gene="guaA"
                     /locus_tag="NBI42_001105"
     CDS             complement(2208..3749)
                     /gene="guaA"
                     /locus_tag="NBI42_001105"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCD1777533.1"
                     /translation="MDMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCKGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(3774..5240)
                     /gene="guaB"
                     /locus_tag="NBI42_001106"
     CDS             complement(3774..5240)
                     /gene="guaB"
                     /locus_tag="NBI42_001106"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCD1777534.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(5278..6546)
                     /locus_tag="NBI42_001107"
     CDS             complement(5278..6546)
                     /locus_tag="NBI42_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCD1777535.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILVLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAIAAIILNFFFNGIKYKQTEENVK"
     gene            complement(6546..7124)
                     /gene="xpt"
                     /locus_tag="NBI42_001108"
     CDS             complement(6546..7124)
                     /gene="xpt"
                     /locus_tag="NBI42_001108"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCD1777536.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(7357..7459)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7639..8046
                     /locus_tag="NBI42_001109"
     CDS             7639..8046
                     /locus_tag="NBI42_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCD1777537.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLETELAIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDNSSHKEVQEHNSAYEEIDITHFAEASKGPKA"
     gene            8189..8851
                     /locus_tag="NBI42_001110"
     CDS             8189..8851
                     /locus_tag="NBI42_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777538.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            8969..9925
                     /locus_tag="NBI42_001111"
     CDS             8969..9925
                     /locus_tag="NBI42_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777539.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTTKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKVLAESTNKVESNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNDKAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMAEPKPQEEKKGFFARLFNL"
     gene            complement(10721..10819)
                     /locus_tag="NBI42_001112"
     CDS             complement(10721..10819)
                     /locus_tag="NBI42_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777540.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVEKAEKG"
     gene            10904..12292
                     /locus_tag="NBI42_001113"
     CDS             10904..12292
                     /locus_tag="NBI42_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCD1777541.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLAYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLVILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FESNDNAALTSH"
     gene            complement(12372..13127)
                     /gene="nfsA"
                     /locus_tag="NBI42_001114"
     CDS             complement(12372..13127)
                     /gene="nfsA"
                     /locus_tag="NBI42_001114"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HCD1777542.1"
                     /translation="MSDHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEMIKENLREVSGQPYVVENGYLFVFAIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            13619..14188
                     /gene="ahpC"
                     /locus_tag="NBI42_001115"
     CDS             13619..14188
                     /gene="ahpC"
                     /locus_tag="NBI42_001115"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCD1777543.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            14204..15727
                     /gene="ahpF"
                     /locus_tag="NBI42_001116"
     CDS             14204..15727
                     /gene="ahpF"
                     /locus_tag="NBI42_001116"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCD1777544.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKELLTEIS
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LPNVDIKTNAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            15842..17461
                     /locus_tag="NBI42_001117"
     CDS             15842..17461
                     /locus_tag="NBI42_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777545.1"
                     /translation="MLDVHFIYPGKLQNACKYFNLPTPKEGFEVTIHALRPENIHKIY
                     HDMDNSEQRFIETLISKGNPVTIVNSRDVNILKLTNHLGFVYQLNENEFFVNNFFLRY
                     INSEVLDNAYGEFESLDTINHMHLNDEAFRVRQHTRLNDVTLEHALMLKTNDELKTIC
                     RQYGIKGFSNKNKQNLVELIMTAFFNNNHIIEQVFTNATLFEFDILSSILESDANYRL
                     NNELIEFEKLQLINFNNFLTSHFYYYYDFEYDCLILPNDVRKHIENFVNQVGNGDFEK
                     AVNQFVDEQTIEKSLAEFENALELSFMDDEVDLDMLLKDDDFEDFGTTDSLRSDAITQ
                     LISEIKKGLIDKKDILPLRIILGSVNLYGILSTNHLLYLTQRFYDATFSKEELQYSLD
                     TLAGTELYYIEDDFVFHPVLFDVVESEASGIDISDEGYYVPASVEELIYYDRHHYLKS
                     DSEIKAFTSYLRKQINIKNDIEKEKYVNEIILLLRTIPTSKLVQPIMQLFIDNAILNN
                     VQNDALLIEKATHARNHLRLWSLQGHRDIPH"
     gene            17533..18159
                     /locus_tag="NBI42_001118"
     CDS             17533..18159
                     /locus_tag="NBI42_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HCD1777546.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINIVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            18424..18807
                     /locus_tag="NBI42_001119"
     CDS             18424..18807
                     /locus_tag="NBI42_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777547.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFASTIITLAIGGKSFDKNDSH"
     gene            complement(18873..19454)
                     /locus_tag="NBI42_001120"
     CDS             complement(18873..19454)
                     /locus_tag="NBI42_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD1777548.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNNVSREEAVDLKVENCKPYIIE"
     gene            19632..19841
                     /locus_tag="NBI42_001121"
     CDS             19632..19841
                     /locus_tag="NBI42_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777549.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILVFLFPTSPHVNLLLF
                     NPILPTFSNIIIHIDHFHANKKDDN"
     gene            complement(19880..20134)
                     /locus_tag="NBI42_001122"
     CDS             complement(19880..20134)
                     /locus_tag="NBI42_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD1777550.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIIFIAIVTLILRAVRKK"
     gene            20428..20691
                     /locus_tag="NBI42_001123"
     CDS             20428..20691
                     /locus_tag="NBI42_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1777551.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(20829..21401)
                     /locus_tag="NBI42_001124"
     CDS             complement(20829..21401)
                     /locus_tag="NBI42_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD1777552.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTVNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(21582..21950)
                     /locus_tag="NBI42_001125"
     CDS             complement(21582..21950)
                     /locus_tag="NBI42_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD1777553.1"
                     /translation="MKTILKTITYLALTIIGAYAALCILKTIDSHGITDQFNPLVKED
                     DSYVKTTEVSTRMDDQLRSYSQSAFNKEGEETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALAKLRR"
BASE COUNT         7466 a         3970 c         3357 g         7259 t
ORIGIN      
        1 gattgtgaat ctatatatga aatagaaaat ttttctaact tcaaatatta gagttgattt
       61 agaaactgaa ctttttaaag atacattact tgaagatcct aatttatctg attatataaa
      121 gaaccaactt ccaacgatat ggagagacta aaagaaatag tttaaattta tttaatttca
      181 ataaaaactc ataataatat ttaagtaagt tagattgctt ttagcattag gaattcgctt
      241 ataattagat gttcaatata ggctttttta cacatacatg aactataaaa agcgaataaa
      301 taggaggtca ttgcgaaatt tgctaaatac aaaattgatg agttccacct aataaaaatt
      361 gttggtttta tagtaacatc tattttcgta ccattcatgt taatttcagt gctaataatg
      421 ctgatataaa gaagaataaa atgattagga gaacactatt taatgtctaa aaatatcaat
      481 caagcaaata gtaaattaaa tacgtcaaac aaaaaactaa aacaagctta ttcaaaatcc
      541 gatagtaaaa acttgaaagt tatatacatg cctcattggc tagagtttta ttcaattgct
      601 atacatagcg atgttacaaa caataaaaaa agatattata aatcatatag tagaggaaca
      661 gtggtatatg taaaactagg gagtaatatt ggaagtgagt tttctggtaa tcatttttgt
      721 gtaatattag ataacaaaga caataaagga aaagaaacag ttacaatagt tccactttct
      781 tccaaaggta ataaaaatca tttgaagtta aacgaatcgg tgctaaatct aacagcaaca
      841 gatttaaaaa aacagattat cgatatcagt agtaaagtac aagcattagc ccaaaaatat
      901 agagatattg acattaaaat tagcacagaa gataaaaagc ttttgtctaa cttaaatcaa
      961 aaagctaatg aactgtatag agtgataggt gtttattcca agcacaaagg gaaagataca
     1021 tacgttaata tatcagcaat tacaacaata agcaaaagac gtataagcaa aataaatgat
     1081 tctgatccta ctggaacgat aataatttca gaagatgaca taaatgaaat cgaaaagcaa
     1141 ttaaagataa agttcttttc taattgaatt gtaatattac atctgatata attgatttac
     1201 aaattcccgg taaccattcc ggcttcattc tcttgaagaa tgacatattc tcagcgtttt
     1261 agctgaaggt cagatgatac gtcatctggc ctcttttttt atataaatgc cctttaagtg
     1321 aaatatacaa caatctgcaa ccaatataga catcacaaaa cacacaataa atttcgcaat
     1381 gtgttataag ccacatgtca ctattatcgt agctagctgc gtcactcaaa acgaatacgg
     1441 aacgcgttgg ttattccaat cataatatta ctcttcaaat acacagccct gtatccgagt
     1501 aaatggatag agatttgaac aaatgaaaac aatcaactaa tggaaaggat aaaatattat
     1561 gcacaacaaa aatgaattca acaaaaaact gaaagatttt ataggtagcg ataaacggat
     1621 ggctttagta aagggttata ttaacgagta taaattaaca actgttttaa gagctttaaa
     1681 taattccgat tacacaaaag ggacgataca cactcgttca atagacaacc taaaacaaat
     1741 agtagataat cgaattattc ctaaaaatat aactcaaaac acaatcttca aattgagtaa
     1801 tttaatcata gaggttaacc tctatgaaag aaacacaatt tatcacggag actttgctat
     1861 ttactatctt gtagaatctg ctctaataat agaaacagat actcctaggc taattaatca
     1921 tataaataac aacccagtcc aaaaaatatt tataataaca acaaatgact ggagctttaa
     1981 tacacttgaa ttagaaaaaa tagtagacga aactataatt tatgatttaa aacaagaaga
     2041 tcctaaacaa tatgaaattt tatataaaaa taaacacgga aaattgcctt attgatgaat
     2101 taaaacgcaa acttagaaca ccttacccct tttccgtcct tattgtaatt ttccaactac
     2161 tcaaattaaa aaccacccgt gcaaacaggt ggttttatta tatataatta ttcccattcg
     2221 attgtgcttg gtggttttga tgtaatgtca tagactacgc gattgacgtg gtctacttcg
     2281 tttacgatac gacttgaaat cttttgtaag acttcccaat cgatgcgtgc aaaatcactt
     2341 gtcatgccgt cgatagatgt tactgcacgg atacctactg tgtgatcata cgtacggtag
     2401 tctcccataa cacctactga ttgaatgttt ggtaacactg tgaagtattg ccaaatttct
     2461 ctttcaagac cttcttctct aatcacttgg cgtaaaattg cgtctgattc tctaacgatt
     2521 tctagtttat cttcagtaat ttctccaagt acacggatac caagaccagg tcctgggaat
     2581 ggttgtctcc atactaaatg ttctggaata cctaattcaa tacctaattt acgtacctca
     2641 tctttaaaca atgtattgat tggttctatt aattcgaatt ccatgtcttc tggtaatcca
     2701 ccaacattgt ggtgtgattt gattgtttgt gctgtcttgg tacctgattc gataacgtct
     2761 gtataaagtg ttccttgcgc aaggaagtct acacctttca gttttgatgc ttcatcatca
     2821 aatacatata caaattcatt accaatgatt ttacgttttt gttcaggatc tgaaacacct
     2881 tttaatttat tcataaaacg atctttcgca ttaacacgaa taatattcat gttgaaccct
     2941 tcaccgaatt gttccataac catgtcgcct tcacctttac gaagtaagcc atggtctaca
     3001 aagatacatg ttagttgatc cccaattgct ttatgtaata atacagcaac aacagatgaa
     3061 tctacgccgc cactcatcgc acataataca cgacggtctc ctacgcgttg acgaatcttt
     3121 tcaatttcga tttcgataaa gttttccatt gtccattgac ctttacaatc acaaacacga
     3181 cggacaaaat tatttaataa atcattacca tattctgtat gacgtacttc tggatggaat
     3241 tgaacaccat aaatgcgacg tttcttatct tcgattgctg catagtctgt acttggacta
     3301 tcagcgataa cttcaaagcc ttctggaatt tcaataactt tatcagaatg actcatccaa
     3361 acagtttgtt ctgctggtaa gccagcgaat aactcatctg acttcgcatt aatgattgct
     3421 ttaccgtatt cacgttcatt ggcacgttca actttaccac ctaataattt agtagttaat
     3481 tgcatgccgt aacaaatacc aagtactgga attcctaaat tatatatttc cggatcaatt
     3541 gtaaatgaac cttcttcata aactgaattt ggaccacctg ataagatgat accttttgga
     3601 ttcattttct taatttcttc aattgaaatt tcatgatcat gtaattcact ataaacgccc
     3661 atctcacgaa ttcggcgtgt aattaattga ttatattggc taccaaagtc taagacaagg
     3721 attaactctt gttcttttgc catatccata tttgtcgttc tcctttatct taattagaat
     3781 gagtagttcg gtgattcttt cgtaatttga atattgtgcg gatggctttc tgctaaacca
     3841 gcaggaccca tacgtgtgaa ttgagcttct tcgcgtaatt ctcttaaatc gtgtgaacca
     3901 gtataaccca taccagcacg cacaccgccc attaattggt aaattgtatc ttgtaacgca
     3961 cctttatatg ccgtacgtcc ttcgatacct tcaggaacaa atttcttagg cgctttgtcc
     4021 tcttggaagt aacggtcgtt tgaacctttt tccatcgcac ctaaagaacc cataccgcga
     4081 tatactttat attgtctacc ttggaaaatt tctgtagcgc ctggactttc ttcagtacca
     4141 gctaataagc tacctaacat aaccgcatgt ccaccagcag ctaatgcttt aatgatatct
     4201 cctgagaatt taataccacc atcagcaatg atagctttac catgtttgcg tgcttcagtt
     4261 gcacaatcat aaattgctgt aatttgtggt acaccaacac cagctacaac acgtgttgta
     4321 caaattgaac ctggaccgat accgacttta acaatgtctg cacccgcttc aaacaaatct
     4381 tttgttgctt ctgcagttgc tacgttacca gctactagcg tgatttctgg gtaagtcttc
     4441 ttaatatgtt tcacttgatc gattacacct ttagagtgac catgtgctgt atcgataact
     4501 aagacatcca cacctgcttc gactaatttt tgagcacgaa tatcagtgtc ttttgaaata
     4561 ccgattgctg cggctacaag tagacgacca tgttcatctt ttgctgcatt agggaattcg
     4621 ataacttttt caatatcttt aatagtaata agaccttcta gacgtccgtc tttaactaat
     4681 ggtaactttt caatcttatg tttttggaga attttttctg cttcttcaag tgttgtattc
     4741 actggagctg taattaaatt ttcttgcgtc attacatcta caattttaat cgagaagtct
     4801 tcaataaaac gtaagtcacg gtttgttaaa atacctacta agttgcgatc ttctttatta
     4861 tcaacaattg gtacacctga aatacggtat ttacccatta atgcttccgc ttcataaacg
     4921 ctttcttctg gcgttaagaa aaatggattt gaaatgacac cattttctga gcgttttact
     4981 ttttgaactt cgtccgcttg ttcttcaacg cccatatttt tatgaataac acctaaacca
     5041 ccttgacgag ccatagcaat cgccatttta gattcagtca ctgtatccat accagcagaa
     5101 ataactggaa tattcaattt aactttgtct gataattgta cgcttaaatc aacgtctttc
     5161 ggtaaaatat cagattgtgc tggaattaat aacacatcat caaacgttaa tgattctttt
     5221 gcaaatttac tttcccacat taaaaacagc ctccattttt caaattaatt agttatatta
     5281 tttcacattt tcttctgttt gtttatactt tattccatta aaaaagaaat taagaataat
     5341 tgcagcgatt gcgcctaaaa caattccgtt ttgagtcaac catgcaaatt gttcacctaa
     5401 acctttaaat gcttgtggta cagcgcttat accagtacct aatcctactg atacagcgat
     5461 aattaataaa ttattttgat ttttaaaatc gatatgtcct aatatactaa caccatatgc
     5521 cattaccatg ccaaacatag ctatcatcgc accacctaac acaggtagcg gtatgatatt
     5581 tgctaatgca ccaagcttag gtatacaacc acatataagt aataacacga ccatgccgta
     5641 tataacatta tttttcttag cgccggataa agaaacaagt cctacatttt gcgaataggc
     5701 tgtatacgga aatgaattga atatagaacc taacactatc gctagacctt ccgcagtata
     5761 acctttacga aaatcttttc tttctaactt cttaccggta atttcactta acgcatgata
     5821 gacacctgtc gactcaatta aactaacgat agctacaata aagaacacta acgtcgatgt
     5881 cacatcaaaa ctgaatcctg agaatctaaa cggcactggg atgcctaacc aaccggcatg
     5941 attgacttga ttaatatcga ccatcccaag taagccagca cctatcgttc ctaaaacgag
     6001 tccaattaat atggcaatac tcttaataaa tccagttgtg aatcgttgta aaacaagaat
     6061 aatgattaat gtcattaaac ctaacaaaat gttcttaaca tctccatagt cctttgcacc
     6121 ttgacctcca gctaagtaat tcattgctac tggcattaaa ttgataccaa tgatagtaac
     6181 aacactaccc gttactactg gcgggaagaa ttttacaaga tgtgaaaaga aaggcgcgat
     6241 gataataact aatatccctg ataaaaatag cgaaccgtaa agtacatcta ttcctttcgt
     6301 ttgaccaatt aaaatcatgg gcgcaacagc cgtgaatgta catccaagaa cgattggtaa
     6361 tcctgttcct gttactttat tggcttgtaa aaatgtggca actccacaca taaatatatc
     6421 tactgtaact aagtaagcga tttgttcagg tgtaaacttc aaacttgtac caacaatgat
     6481 tggaactaaa atggcacctg cgtacatagc taaaagatgt tgaacactta ggattaaatt
     6541 tttcattatt cttctcccac caatgtcact ttatttcctt ctagtgaagc aaccttgcag
     6601 agagaagaaa ctgttaaacc tgcttcttct aaacgttgat gcccattttg gaaactcttt
     6661 tcaacaacaa taccaatacc agctgtctta gcattcgctt gctgtgcgat atcgtataat
     6721 cctaatgaag catcaccatt tgctaaaaag tcatcgatga tgagtacagt atcttcttct
     6781 gataaaaatt cttttgaaac aatgaccgta cttgttttat ttttagtaaa tgaatgaata
     6841 gatgtttcat aataaccatc tgtcaaagta ctaggctttg ctttttttgc aaataagcat
     6901 ggtacatcaa aatgaagtgc agccataatt gctggtgcga tacctgaagc ttcaatggtt
     6961 aaaattttag tgattccctt atctttaaac tgctcgtaaa aagtacgccc tacctcattc
     7021 attaactttg cgtcaatttg atgatttaaa aatccatcga ctttcaatat tttttcatca
     7081 atgacaacgc cgtcttcttt tactttttgt cctagtaact ccactactaa aacctcctca
     7141 gatttgtgtg aaacattttg ctcttaaaat tagtgctaga tacaaaaaaa tccccaaact
     7201 aaataatagt ttcagggttt atgagtgaac gaacatgcat cacgaatttg tcatgcaatc
     7261 aatgtaaaag aagtttcatc aagtaaactg tctccaatca tgaaaatgat acaaatcatc
     7321 cattcatggt tacaatttaa tggctgaagc tactcctagt attgtgttgt tactcatagt
     7381 catgtcgttc aaggcaacat ggtagaaact tctaaagcca tattctttag attatatgag
     7441 tttatgtaaa ttatttaacg ataatagcaa attttcggca ttttttcaat aactgcttat
     7501 gtaaactttt aatagattta taaacgtgca aatacatata gtgaaaatga aagcttataa
     7561 tttaataatg aaagggtttt actttataat aagaagggta aaagattaaa taaacaaatt
     7621 ttaaaagggg cgacaactat ggcagatatt actgtagtaa atgacactgg tgaattatac
     7681 aacgtaatca atcaaaaaaa atctgaaggt tatttagaaa cagaattagc gattataagt
     7741 aaaagtaaat tgcatttaaa tgacttacac gattctgaaa tctcccttat ctcaacaagt
     7801 ggcacattta gcgatagaat gactaaattg ttaacaggtg aagatggtga acatgctgtc
     7861 ttatcaagat ataatttagc acctgatgaa ttagaaaaat ataagcaact tattttagat
     7921 gacaaaatgt tagttgtagc tgtgcgtgac aattcatcac ataaagaagt tcaagaacat
     7981 aactcagcat atgaagaaat tgatattact cattttgctg aagcgtcaaa gggtcccaaa
     8041 gcttagttat cacactgaat tcaaaatgag attactgtac tgtctaattt aagatagtac
     8101 agttttttat accatttaaa agtttatgaa cagtctttta caccaggcga ttatgctata
     8161 atattattta aattggggag gtgttcttat ggaatttaaa accattcaat ctgtcaaaga
     8221 tccacattac aacgaagcac ttaaacttta cgatactaag ttaaatttat ctttaactga
     8281 agataaacgt atcatcaaac gctcattaga aaacaacaaa actgaaaacg attatgtctt
     8341 tatcgtaggc ttagaaaacg atgtcgttgt cagtctagca actgcacatt atgaagccac
     8401 aaccaattct gcatttttaa tttatttatt cgcaaaagac ctacccaacc atgatgagtt
     8461 aatgacacaa acattacatc aaattgaaca tgaattaaac ttattatcaa accttgttca
     8521 ttctagaagt attaactttt taatgatgga agtccctaaa gaacctgcag atttaacaca
     8581 agataatgga ctcattattg aacaccgtag acagttttta tacgaacatc aatttgaaag
     8641 acaaactgaa attgattaca ttcatccaac taatgatggt cacactaaaa aagtggactt
     8701 atttttaaag aaaaatatta aactgacaaa ggatatctac ggtacaagtg ttaaatcaaa
     8761 ttatattttg aagtatgtat ttgcaaatgg tatcgcacgt gaaattattt atgctttatt
     8821 agaagaaatg gatttacgta aaccatttta aacaaacttg aaatcactaa caataacgtt
     8881 taaactatgt atgaagcaag actgacgttt cactgcccgt agacaaacgt cagtatacat
     8941 aaagtatatg cagaaaggac tttaaagtat gttaactaaa gaatttgcac aacgcgtaga
     9001 actaagtgag aagcaagtcc gtaaaattgt tcaacattta gaagaacgtg gttaccaact
     9061 aagcaagact gaatatcgtg gtcgtgaagc aactgatttc aaagaagaag atatcgagct
     9121 tttcaaagac attgccgaca aagtaaaaca aacaaatagt tatgatctag cgtttgatga
     9181 attagaaaaa gaaaaagact tcctgcaagt cattgtcaaa aacgatgaca aaaacttacc
     9241 tactaatcaa aatgtcgctc aactagtaga agatttacgt ctagaaatcc agaaaatgcg
     9301 cgaagaacgt cacctacttg gtcaaatgat gaatcaagta catcagcaac aacaagaatt
     9361 aaaagaactt caaaatcaac ttacaactaa aatcgattca aatagtgaat ccttaaaagc
     9421 catccaaaca tcacaagagg ctatccaaga agcacaagca tctcaagcta aagtattggc
     9481 tgaatcaaca aataaagttg aaagtaatgc tgttactgaa gataaagctg acagcaaaga
     9541 ttcaaaggtt gctggagtga atacatcaac agacgccaag acggatacta aagcagaaaa
     9601 tgcaggcgat ggcactgcta ctaaagtaga taaagaggat caaatttctg caactgaagc
     9661 aattgaaaaa gccagcgttg agcagtctaa aaatgacaaa gcagctgaga cttcaaataa
     9721 agaagctaca gtagatgcag aagcgcaaca tgatgctgaa caacaagtag cagaagccca
     9781 tgcagaagct tctaaacaag ctacttcgaa cgattcattg gaagcgaaag cagaaaatga
     9841 cagcacagct tcacagtctg aaatggcaga acctaaacct caagaagaga aaaaaggttt
     9901 cttcgcacgt ttattcaacc tgtaaacttg tgatttgaac acaaatttaa tactcaataa
     9961 tatatatttc tatgatttaa aaactgatct tttataaacc tatagaactg taatttattt
    10021 tcaaatttaa tactcaattc ttaaaacctg aaacaagctg atatactcct ttcaatatct
    10081 actttgaaga gagcatatct aactaatttg tttcaggttt tttaattatt cattatagtt
    10141 aatattgaat tttgaataaa tttttgtagt atgttacttt taaatgggat ggcttctgtg
    10201 tattcctaaa tatattcatc ttatgagccc attcaattta aatgtttggc tgtgttagta
    10261 actgacgaat cgcacaaggg cacactcata ttcacattta ctacaaacgc ctttttgctc
    10321 aaacaaaaat atttgtttat ggtagctttt gaactaccac ctgcacacac gaaccctcac
    10381 aagatgctaa ttttgattcg acagaatttt ttacaacgtg tttgttcgaa gcgtcttttc
    10441 aaggttgatc atttattatc ttttttattc aataaatatg aggttagcaa tttttatcaa
    10501 accgccatag ccacatctcg attcaaggat acatttgttt tctcatatct tatctctcat
    10561 gacagtacac gatatatatc ttttccaaat attcatcact tttttcctgt taaaatcatt
    10621 tctattatta acgctgtaat gacagcttat ttaatttatt gcatttaatg gcctgcttca
    10681 ctttcaaata tcaactttaa tcgactagct catcacgcta ttaacccttt tcagctttct
    10741 ctactgaatt caaaacgatt tgtttacaaa ttcccttatg ccaacggtcg aatttcatca
    10801 tgtttacatt taattgcaaa tgccctcttg aaataattta aaattaatat tattcttagc
    10861 atactagtcg gaattatatt ctaaaatttg aggagtgacg ttcatgaatg cttttcttac
    10921 actgatcaat attattgtat tggtgatttt catcgtaata ttgcacatga tggctcgtaa
    10981 acatatatct tttgcgaaac gtgtgtttac agctttaggt atcggcattg tatttggcgt
    11041 gttactacat cttgcctatg gcacacattc taatgtcatt acatcaacaa gtgactggtt
    11101 caacattgtc ggacaaggtt acgttgcatt gttgcaaatg attgttatgc cattaatttt
    11161 catttctatt gtcgctgcat tcactaaaat tcaaatcgga gaaaagtttg ctaaaattgg
    11221 tagtctcatt tttatctttt taattggcac tgtcaccatt gctgcaattg ttggtgttgt
    11281 ctatgccttg gtgtttggtt tagacgcatc aacaattaat cttggtaacg ctgaacaagc
    11341 acgtggaagc gaaattgcta aacaagcaaa ggatttaaca gcacatacgt taccacaaca
    11401 aattttggaa ttattgccta aaaatccatt cttagatttc actggacaac gtgcaacttc
    11461 cacgattgct gtcgtgatat ttgcttcgtt tataggattt gcttatttac gcgtagcacg
    11521 caaacaacct gatcatggtg aattgcttaa gcgtgcgatt gacgctatct attcactagt
    11581 aatggcaatt gtgacgttcg tcttacgttt aacgccatac ggtgtattag caattatggc
    11641 caacacatta tcgacaagtg actttggcgc catttggact ttaggaaaat tcttaatcgc
    11701 atcgtacgct gcattaatta cgatgtatat cattcactta gttattttaa gtctgttagg
    11761 tattagccca atacgctatg ttaaaaagac tttggaagta ttaatctttg cattcacatc
    11821 acgctcaagc gcgggtgcct taccactgaa tgtacaaaca caaacaagac gtctaggcgt
    11881 gccggagggt attgccaatt tcgctgcgac atttggattg tcgattggac aaaatggttg
    11941 tgcaggcatt taccctgcca tgcttgcaat tatggtcgca cccgtcgcta atgtagaaat
    12001 tgatttacaa tttattgtaa cgttaatcgc tgtcgtgatt attagttcat tcggcgttgc
    12061 tggcgtcggt ggcggggcta catttgcatc catattggtg ttgtcaacgt taaacttgcc
    12121 agtcgcttta gccggcgtgc tcatctccgt tgaaccattg attgacatgg gtagaacagc
    12181 attaaacgtg aacgattcaa tgctagctgg tacaggtacc gctaagttaa ctaaacattg
    12241 ggacaaagat actttcgaat caaatgataa tgctgcatta acttcacact aattcattgc
    12301 aagaaatacc cgccccctct agctatactt atcattttaa gctagagggg gcgggttact
    12361 ttggtgtctt gctatcgctg tattaagcct gatttttgca attgttctaa catatctaat
    12421 cttgctttgt ttcctaggaa catctcaatt tgctgcgacc atgtttcttt gcgattcccg
    12481 tttgtacgtt gatcatagta ctcgctgatt gtttgatcgt aatctgccat ttgtgcatac
    12541 tgtgtttcct tatcagcatg atacttatta tgatggaaga catggtcaaa tggtaagcgt
    12601 ggcttggctg caccattttc gtcatctgcg ggttccccta ctgccatccc aaataccggg
    12661 aagacatagt caggtaagtc taaaatttct cgaacgcgtt caacatcatt tcttaatgat
    12721 cctaaaaaga caatgccata ccccatatct tcagcagtta ccgcaatatt ttcggcaact
    12781 aatgctgcat caattgctcc tactagcaaa ccttccgttg aaccatatgc attttccata
    12841 tcagtttcag catgttgatc aactaaatga tgacgataat aatcaatagc aaagacgaat
    12901 aaatagccat tttctacaac ataaggttga ccagaaactt ctcgtaaatt ttctttaatc
    12961 atctcatcgt cgataccaat aattgagtat gcttgtagga aacttgacgt cgaagcgctt
    13021 tgtccagctt ctaccaattt cttaacaacg tcttcactta aaggtttatt cttaaatttt
    13081 ctaacagaat gatgcttttt cacaagatta tatacatgat ctgacacgtt acccactcct
    13141 cgatatttta caattaatta aaaatcttca tattgcaatt gtacacgtcc aaactttttt
    13201 atagcaatta tttgcttata agttaataat tgaactttca aaaatattgt ctcgtactta
    13261 aaaaatccag tttctcacgc attaccttca tccatctcga acaaatattc tcacaccatt
    13321 atcaataagg ttgacttagt tgagaataca aatcttcttt ttttacattt tttacattaa
    13381 aatcaagata ttaaatatag acacaacctt ttaaatcatc gactacaaaa tccattatca
    13441 aagcacttta tattatcttt ctttatttta aaagtaatca ttcactttac aaagttatcc
    13501 agttgactat tctggatcaa aatgtaataa tgctaactaa gagattagaa ttattataat
    13561 taagcacaat caattgatag ttctaattca tacttaaatt cttaggagga agatatttat
    13621 gtcattaatt aacaaagaaa tcttaccatt tacagcgcaa gctttcgatc caaaaaaaga
    13681 tcaatttaaa gaagttacac aagaagattt aaaaggttct tggagcgtag tatgcttcta
    13741 tcctgctgac ttctcattcg tttgtccaac tgaattagaa gacttacaaa accaatatga
    13801 agaattacaa aaattaggcg taaatgtatt ctcagtatca actgatactc acttcgtaca
    13861 caaagcatgg catgaccatt cagatgcaat tagcaaaatc acttacacta tgattggtga
    13921 cccatcacaa acaatcactc gtaatttcga tgtattagat gaagctactg gtttagctca
    13981 acgtggtaca ttcattatcg acccagacgg tgttgtacaa gcatctgaaa ttaacgctga
    14041 cggaattggc cgtgacgcta gtacattggc tcacaaaatc aaagcagctc aatatgttcg
    14101 taaaaaccct ggcgaagtat gcccagctaa atgggaagaa ggcgctaaaa cattgcaacc
    14161 tggtttagat ttagtaggta aaatctaagg aggcattaca accatgctta atgctgattt
    14221 aaaacaacaa cttaaacaac tattagaact aatggagggc aacgttgaat tcgttgccag
    14281 ccttggttca gatgataaat ctaaggaact caaagaatta ttaacagaaa tttctgatat
    14341 gtcacctaga ctttcacttt ctgaaaagtc tttaaaacgt acaccaagtt tctcagtcaa
    14401 tcgtcctggc gaagaaacag gtgtaacatt tgcaggtatt ccattaggtc acgagtttaa
    14461 ctcacttgtt ttagcaattt tacaggttag tggtcgtgca cctaaagaaa aacagtcaat
    14521 cattgaccaa attaaaaact tagaaggttc attccatttt gaaacattca ttagtttaac
    14581 atgtcaaaaa tgtcctgatg tcgttcaagc acttaactta atgagtgtga tcaaccctaa
    14641 catcacgcat tctatgattg atggtgcagt gttccgtgaa gaatctgaaa acatcatggc
    14701 agtccctgct gtctttttaa atggcgaaga atttggcaat ggtcgtatga caatccaaga
    14761 tattctttcg aaactaggca gtacggcaga tgcatctgag tttgaaaaca aagaacctta
    14821 tgatgtctta atcgttggtg gtggtcctgc tagtggtagt gcagcgattt acacagcacg
    14881 taaaggttta cgtactggta tagttgctga tcgtatcggt ggccaagtta atgatactgc
    14941 tggtattgag aacttcatta ctgttaaaga aacaactggt tctgaatttt cttctaactt
    15001 agcagcgcac attgatcaat atgacattga tgcaatgaca ggtatacgtg ctacagatat
    15061 cgaaaagact gacgaagcaa ttaaagttac gttagaaaac ggtgctgtct tagaaagtaa
    15121 aacagtcatt atcgctactg gtgcaggttg gcgtaagcta aacattccag gtgaagagca
    15181 attgattaat aaaggtgttg cattctgccc tcactgtgac ggacctctat ttgaaaataa
    15241 agacgtagca gttatcggtg gcggtaactc tggggttgaa gcagcaattg accttgctgg
    15301 tatagttaat catgttacat tattcgaatt cgctagcgaa ttaaaagcag acaacgtgtt
    15361 acaagatcgt ttacgttctt taccaaatgt tgatatcaaa acaaatgcca aaactactga
    15421 agttgtcgga gaagaccatg ttacaggtat acgttacgaa gacatgagca ccggcgaaga
    15481 acatctactt aacttagatg gtatctttgt tcaaattggt ttacttccaa acacatcatg
    15541 gttaaaagat gctgttgaat taaacgaacg tggtgaaatt gttattgatc gtaacaataa
    15601 tactaacgta cctggaatat ttgctgctgg cgatgtcaca gatcagaaga acaaacaaat
    15661 tattatttca atgggcgctg gtgcaaatgc agcattaaat gcctttgact atattatcag
    15721 aaactaaagt tttcacagtt tcactgacag tatcttacat ttgttaagat gctgttattt
    15781 ttttatcccg acataaaatc atgttatgat aaacttaata tatacaacga ggtgtgcaaa
    15841 aatgcttgat gttcatttta tatacccagg aaaattacaa aacgcatgca aatattttaa
    15901 tttaccgacc ccaaaagaag gatttgaagt cacgattcat gctttacgcc ctgaaaatat
    15961 acataaaatt tatcatgata tggataattc agaacaaaga tttatagaaa cattaatatc
    16021 taagggtaac cctgtcacaa tcgtaaatag ccgtgatgtt aatattttaa agctaacaaa
    16081 tcacctaggt tttgtttatc aacttaacga aaatgagttc tttgtaaata atttcttttt
    16141 aagatatatc aattcagagg tactagataa tgcatatggt gaattcgaat cgttagatac
    16201 aataaaccat atgcatttaa atgatgaagc ctttcgtgta agacagcata cgcgcttaaa
    16261 cgatgtcaca ttagaacatg ctttaatgtt aaaaactaat gatgagctaa aaacaatatg
    16321 caggcagtat ggtatcaaag gattctcaaa taaaaacaaa caaaatcttg ttgaattaat
    16381 tatgacagca tttttcaata ataatcacat cattgaacaa gtgtttacta atgctactct
    16441 atttgagttt gatattcttt ctagcatact tgaaagtgat gctaactacc gattaaacaa
    16501 tgaactaatt gaatttgaaa agctacaatt aataaatttt aacaactttt taactagtca
    16561 tttttattat tattacgact ttgaatatga ttgtcttatt ttgccaaatg atgtgcgtaa
    16621 acatattgaa aatttcgtga atcaagtcgg caatggtgat tttgagaaag ctgtaaatca
    16681 gtttgttgat gaacaaacaa ttgagaaaag tttagcagag tttgaaaacg cgttagagtt
    16741 atcatttatg gacgacgaag ttgacttaga tatgttactt aaagatgatg actttgaaga
    16801 ttttggtaca accgatagct taagaagtga tgcaatcact caattgattt ctgagattaa
    16861 gaaaggatta atagataaaa aagatatttt acctcttcgt attatacttg gttctgtaaa
    16921 cttgtatggt attttatcaa ctaatcatct actatatctc acacaaagat tttatgacgc
    16981 tacttttagt aaagaagagt tgcaatatag cttagatact ttagctggta ctgagctata
    17041 ttatattgaa gatgacttcg tgtttcaccc tgtgttattt gatgttgtcg agtctgaggc
    17101 gtcaggcatt gatatttctg atgaaggata ctatgtacca gcttctgttg aggaattaat
    17161 ctattatgat agacaccact atttgaaatc tgattcagaa attaaagcgt tcacaagtta
    17221 tctacgcaaa caaatcaata taaaaaatga tattgagaag gaaaagtatg tgaatgaaat
    17281 cattttgctt ttacgtacga tacctacgtc taaattagta caaccaatta tgcagttatt
    17341 tatagataat gccatcctta acaacgtcca aaatgatgca ttattgattg aaaaagcaac
    17401 tcatgcacgt aatcatttac gtctatggtc attgcaaggt catcgtgaca ttccacacta
    17461 attcaacgtt actctattag catgaataca tattttttca attaaagata aactaaggag
    17521 ctatattaca ttatgaaaaa gagattacta ctaagtacat ttttagcatc gacattaatt
    17581 cttacaggtt gcgcgtccga tcaatctgat aacgaagatc atcacacaag tactggcatc
    17641 catgcaccta aaagcgctaa aaaattagaa acaaaagata tctttaactc agataagaaa
    17701 aatagtgata ttagtgatgc agagatgaag caagctatag aaaagtattt gtctgtaaat
    17761 agcgatatac ttgataacaa atatattatg caacataaac tagataaaca aattgacagt
    17821 caaacaaaag tgactgaaaa acaagcagaa acacttagcc acttatctaa cttagctgtt
    17881 aaaaacgatt tgcatttcaa gaaatttgtc actgaaaaca atattcctaa agaatataag
    17941 aaacccgttg aattaatgat gaattacttc aaagctttaa atagtacaat cgccaatgtt
    18001 gatgaagata ttgaaaagct tagctatcaa ccgcaaaata aaatcaatat tgtcgacgtg
    18061 ccaacaaaat atgccggtga tgttaataaa aagcaacaag ataaaattaa agatttctta
    18121 aaatctaaag gtatcaagag cgatgttatc gataaataac tgaaagccat cattgcgaca
    18181 tgtaacacat cattacatga gttttaattc atgacaattc ttcatcttta aaagtgacta
    18241 atctttgaaa caataaattt taaccaatac cattttgcat caagcagcat attactgttt
    18301 gatgcttttt taaatgctct taaattctct atccctccta aaatccccat tagttgacgc
    18361 atttgactat atccctaaca tattccatac ttatattgac tctataaaca aaggatggtc
    18421 atgatgaaag ttcgaatcac ttttattatt ttagcaattt tatcgacgat tgtttgcttg
    18481 ttcttagcag caatgcatcc aacaggacca aacacagtaa cttttgaaca gccatattta
    18541 ttcaccctca acatcattat aatggtatta gtcgcattac cttcacttgt tttagctata
    18601 tatgattata tgagttttag aatttcttct gctattttac aatttctagg ggctatctct
    18661 tggttctttt tatcattgat attatcgctc acacaattta caccttttac attagcgtca
    18721 tttataactt caattatttt gttcgcaagc acaattatca cattagctat tggtggtaag
    18781 tcttttgata agaatgattc ccattaaaat ccaaatacaa aaaaggttct gaaggtcgct
    18841 ataaaacaca gcttttcaga acctctatac ttctattcaa tgatatatgg tttgcaattt
    18901 tcaactttta aatccacagc ttcttcccgt gaaacgttat taaaataaac cattaaacaa
    18961 cgaatgacaa cttgatgtgc aacaatgacg atatcatctt tttgtgtatc ttcattgaca
    19021 acatgattca taaaatgttc gacgcgttga tatacatctt cataactttc tccttcaggc
    19081 gctttttgtg aaaaactatg acgaaagtct ttaaagtttg gatcattgaa atatttttca
    19141 tatttcggat tcgcactgat ttcatcttta tattcaccct caaatacgcc aagtgaacgt
    19201 tctcttaata gaggcgtagt cgttgatgca atgtcatatg gaaaaatatg ttcaaacgtt
    19261 tgctgtgttc ttaataagtc tgaaacatat acatgtttaa tctgtttctc tttaaaataa
    19321 tcacataaat cgtctgcact ttttgtgcca gtatccgtta acggcacatc taattgtcca
    19381 caaaaataag atctaaaatg tttattgtca taattcgatt ttgattcgcc atgtctaact
    19441 aaataaatcg tcataatatt actccttacc ttatgtattt catatctacc ataacacttt
    19501 gactactaat tcgatatcaa tcttaatatt ctattctaaa aaaataatta attcatattt
    19561 taaattaatg actctaaaaa tagtattgtc tttaatcaag tgatgtgtta ctatatgggt
    19621 gtagttctta aatgactaca cagtctttta tattcatgaa cataatactc ctggatggcg
    19681 caacccaacg tcatcctttt tttatttgtc aaatacttgt ttttttattt ccaacctctc
    19741 ctcatgttaa tttattactt tttaatccta tcttacctac attttcaaat attataatac
    19801 atattgacca ttttcacgca aataaaaaag atgacaactg aatgccatct ttatattaat
    19861 tcaacctaag ttgaatattt tattttttac gaaccgctct taaaattaac gttacaattg
    19921 caataaagat aattgaacca attaaagctg gtaagatgta aatacttcct aattcaggac
    19981 cccattgtcc gaatagtttg ccacctaccc atgatccaat aatacctgcg ataatattac
    20041 ctaaaatacc acctgggata tctttaccca taatagcacc agcagcccat ccaattaagc
    20101 caccgacaat taacattcca ataaatccaa acataatttt cagtctcctt tttctattta
    20161 ttttgcgtta ttctaagtag tacccattat tagactttct aaaacaaatt caaattattt
    20221 ttatctaaat atttttcaaa gtagtaattg aatatcaatt ttattcaatg tagctatcgt
    20281 tatttaaagt ctctgtaccg gcaatatcat atacattatt ttttctgccg aattcatagc
    20341 ttgattattt tatgttatag gactagaata tacacatatt attagagcat ctttgaattt
    20401 taaatcaaga agcgaggtta atgaacaatg aatatgcata ttttatataa cttacgaact
    20461 aaacataatt tagaaattga cgaattagca caacaattaa atgagaaata tggtactaaa
    20521 tatgaagcac atcaaatttg ggaatgggag aatcatcacc atgaacctaa atttaaagat
    20581 gccatgcatt tagctgactt ctttgatgca ccatatgaaa tgtttttaga aagtaaggtt
    20641 aaagaatatc agaaacattt agaagaagtc gatattcgca tggataaata gatgcaaata
    20701 aaccctcaca acacgtttgg catatatcct ttcaaatcta tacgggatat attacattgc
    20761 gttgtgaggg tattttaata attaatatga attaagacat gttattatca ttagtattac
    20821 gacatatttt agtgatcttg ctcactcttt aatactttac cgtttttagc atcaacagta
    20881 acttcttgtt ttttattacc ttttttcaaa tcgatattgt aaacaagttt gccatcatct
    20941 ttttcaagtg accattcttt aatatcacca tcaaattctt tttgtccttc tttaatggct
    21001 tttttgtaat ctatagcatc gctatattta aagttatcat tttcattcac tgtatcttct
    21061 ttttcagtct ttttgttaat cactttttta tttttatcag caacaagtac ttctgactct
    21121 tcaccagatt tttgttgtgt cactttatat gcccattcac cattagaatt ttcaaatgaa
    21181 attcctttca acttttggcc tttgtaagtt tcttcagctt ttttcacagc atcttctggg
    21241 cttgttttaa catcttttaa tgcaataaca tcttttgttg tattagtgtc ttgattagta
    21301 tttgactctg ttgattttgt ttcatctttt ggagtatcat tgccacatgc agtaagcacc
    21361 accgctgaca ttgataacac tgctaatgat tttaatttca taatatcact ctctcttcct
    21421 ttttttgaaa ctcataacaa aagcttatat gctatataga ttgtattacc ccttgttttt
    21481 aattctattc ataattatta caaatttttt taaattactc ttcatgtgtc acttcatttc
    21541 gtactcatct ttaaaatgaa ccatgtaata taaacccgtt attatcgcct caatttggct
    21601 aatgctttct ttggtacttc ttccttttca acttcttcaa aagtttctac atgatgacct
    21661 ttatgtgtaa ttttcaagta tctatgcggt ttaacatcaa atgtagcagt atacattaat
    21721 tgcgtctctt ccccttcttt attaaaagca ctttgactat aacttcggag ttgatcatcc
    21781 attctagtag acacctctgt cgttttaaca taagaatcat cttcctttac taatgggtta
    21841 aattgatctg ttataccatg agagtctatt gtttttaaaa tgcataaagc agcataagcg
    21901 ccaatgatag taagcgcaag atatgttatt gtttttaaaa tcgttttcaa aagatcacct
    21961 ctgacaaaat ataaaatagg atgtctctga aaacaactta cataattgta attaaaatgt
    22021 aagtattagg agaaaatgaa ggtatttgaa tc
//
LOCUS       DAHHRR010000034        21791 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.034, whole genome shotgun sequence.
ACCESSION   DAHHRR010000034 DAHHRR010000000
VERSION     DAHHRR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21791
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.034"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(77..421)
                     /locus_tag="NBI42_001126"
     CDS             complement(77..421)
                     /locus_tag="NBI42_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777554.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            complement(552..851)
                     /locus_tag="NBI42_001127"
     CDS             complement(552..851)
                     /locus_tag="NBI42_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1777555.1"
                     /translation="MMTKDERIRFYKSKEWQITRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            complement(1083..1499)
                     /locus_tag="NBI42_001128"
     CDS             complement(1083..1499)
                     /locus_tag="NBI42_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1777556.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            complement(1527..1727)
                     /locus_tag="NBI42_001129"
     CDS             complement(1527..1727)
                     /locus_tag="NBI42_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCD1777557.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLEEYRIELKRMNNDIKK"
     gene            complement(1727..2377)
                     /locus_tag="NBI42_001130"
     CDS             complement(1727..2377)
                     /locus_tag="NBI42_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777558.1"
                     /translation="MNEQIIGSIYTLAGGVVLYSVKEIFRYFTDSNLQRKKINLEQIY
                     PIYLDCFKKAKKMIGAYIIPTEQHEFLDFFDIEVFNNLDKQSKKAYENVIGFRQMINL
                     SNRVKAMEDFKMSFNNEFSTNQIFFNPSFVMETIAIINEYQKDISYLKNIINKMNENR
                     AYNHIDSFITSEYRRKINDYNLYLDKFEEQFSQKFKINRTSIKERIIINLNKRRFK"
     gene            complement(2536..2685)
                     /locus_tag="NBI42_001131"
     CDS             complement(2536..2685)
                     /locus_tag="NBI42_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1777559.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            complement(2682..2888)
                     /locus_tag="NBI42_001132"
     CDS             complement(2682..2888)
                     /locus_tag="NBI42_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCD1777560.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKERY
                     EAQVKRGAVIKVGQLFENIRECGK"
     gene            complement(2925..3458)
                     /locus_tag="NBI42_001133"
     CDS             complement(2925..3458)
                     /locus_tag="NBI42_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCD1777561.1"
                     /translation="MNNLTVDQLKELLQIQKEFDDRIPTLDLRDSKIAYVVEFFEWFN
                     TLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNTEFH
                     KMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIVIDIAYNLYSIDQLIDAYKKKMK
                     RNHERQDGTADAGKGYV"
     gene            complement(3473..3634)
                     /locus_tag="NBI42_001134"
     CDS             complement(3473..3634)
                     /locus_tag="NBI42_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777562.1"
                     /translation="MLEIIDQRDALLEEKYLNDDWWYELDYWLNKRKSESEQIDIDRV
                     LKFIEELKR"
     gene            complement(3635..3916)
                     /locus_tag="NBI42_001135"
     CDS             complement(3635..3916)
                     /locus_tag="NBI42_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777563.1"
                     /translation="MEWKRLKNVVPHPVIKNKNLKSVYVTKDNVKEVQKELGFFEIFN
                     EEVLLTGFLSFQRIPIYIIWINPKSHKTPRYYFANEHEIERYFEFLEDE"
     gene            complement(3917..4090)
                     /locus_tag="NBI42_001136"
     CDS             complement(3917..4090)
                     /locus_tag="NBI42_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777564.1"
                     /translation="MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIID
                     FITKPIYLIKGDE"
     gene            complement(4080..4331)
                     /locus_tag="NBI42_001137"
     CDS             complement(4080..4331)
                     /locus_tag="NBI42_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCD1777565.1"
                     /translation="MNNREQIEQSVISTSAYNGNDTEGLLKEIEDVYKKAQAFDEILD
                     GMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQENEY"
     gene            complement(4324..4689)
                     /locus_tag="NBI42_001138"
     CDS             complement(4324..4689)
                     /locus_tag="NBI42_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1777566.1"
                     /translation="MNYETGFQLGVMEARLKKMRKQRDACKKQRDELIVDIAKLRERN
                     EELENMWRTVKNELLGRYEHYCFKIRELHPESKANRIGALYIGGKSTADIILSRMEEL
                     DGTNEFYEFLGQMEEDTNE"
     gene            complement(4704..4946)
                     /locus_tag="NBI42_001139"
     CDS             complement(4704..4946)
                     /locus_tag="NBI42_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HCD1777567.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELEIYIKQHGLEYEEQKELTLF"
     gene            complement(4950..5318)
                     /locus_tag="NBI42_001140"
     CDS             complement(4950..5318)
                     /locus_tag="NBI42_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777568.1"
                     /translation="MARKARIVTINDKPYRFSKFEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKREAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            complement(5331..5735)
                     /locus_tag="NBI42_001141"
     CDS             complement(5331..5735)
                     /locus_tag="NBI42_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCD1777569.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYVHTYMPTNYTKHKKYL
                     QSQMPRLNLENALKIELAFYFTPPESWSKKKKAQAIGQLKVTKPDIDNLIKTVLDACN
                     GHVWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            complement(5744..5962)
                     /locus_tag="NBI42_001142"
     CDS             complement(5744..5962)
                     /locus_tag="NBI42_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777570.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGIKETKFNGRRW
                     YLDDYELNTLIDNEQMELGHQTNLFEYI"
     gene            complement(5969..6862)
                     /locus_tag="NBI42_001143"
     CDS             complement(5969..6862)
                     /locus_tag="NBI42_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCD1777571.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KAAAFDFFQDNGFGFITPYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQIESKKQTYKPHVKQSKEKTPKWLTDGTRETKTAE
                     VDENLEKDREAFIKRLNSKWE"
     gene            complement(6892..7362)
                     /gene="ssb"
                     /locus_tag="NBI42_001144"
     CDS             complement(6892..7362)
                     /gene="ssb"
                     /locus_tag="NBI42_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1777572.1"
                     /translation="MLNRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVVADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANCPIEIDDNDLPF"
     gene            complement(7363..7980)
                     /locus_tag="NBI42_001145"
     CDS             complement(7363..7980)
                     /locus_tag="NBI42_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1777573.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKIL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSALANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKREIQKVTGVPVYVGGL"
     gene            complement(8061..8981)
                     /locus_tag="NBI42_001146"
     CDS             complement(8061..8981)
                     /locus_tag="NBI42_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCD1777574.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            complement(8983..10938)
                     /locus_tag="NBI42_001147"
     CDS             complement(8983..10938)
                     /locus_tag="NBI42_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1777575.1"
                     /translation="MMMDIKINKLTISNFAGIKEESFNFDGKDTKIYGNNATGKTTTA
                     TALQWLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPK
                     YTINQKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLD
                     WKKRRSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIK
                     DIPIRINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGAEEINLRNQLADKQ
                     SELKRIEDNNSASNENKIHTLTNELHVENGTVANLKTRLNQNKQQIAHEENRRNQLLE
                     NHKGLKSDLEKAKNQKFEYLDDNVCSCCGQQLPAEQVNEAREKALQKFNASKSKELET
                     IQTSINHIISEGKKIKPIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVT
                     QTDEYKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNK
                     HLDDVISELRNEEDRLLDEKEKYSHNLYILKEFTTTKVKMLTENINNEFDIAEFKLFN
                     TLVNGELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESV
                     TELIKTESQQIQLIVNEQDKKLRMETI"
     gene            complement(10935..11198)
                     /locus_tag="NBI42_001148"
     CDS             complement(10935..11198)
                     /locus_tag="NBI42_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777576.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            complement(11207..11467)
                     /locus_tag="NBI42_001149"
     CDS             complement(11207..11467)
                     /locus_tag="NBI42_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCD1777577.1"
                     /translation="MYYKIGDVCQKVINVDGFDFKLAVKKQDYSILVNVLDLEDRFID
                     SINITDENDLYTALDILNQSIYEWIEENTDERDRLINLVMRW"
     gene            complement(11560..11721)
                     /locus_tag="NBI42_001150"
     CDS             complement(11560..11721)
                     /locus_tag="NBI42_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCD1777578.1"
                     /translation="MSNIYKSYLVAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            complement(11718..12038)
                     /locus_tag="NBI42_001151"
     CDS             complement(11718..12038)
                     /locus_tag="NBI42_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCD1777579.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDENFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            12097..12729
                     /locus_tag="NBI42_001152"
     CDS             12097..12729
                     /locus_tag="NBI42_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777580.1"
                     /translation="MYIDPLKNVRFSINNVISNVEISKSMAIKQSLKPKYQLDIINRN
                     NINLFSDFKVDFHLNNLIEMNFNLRNSFSSLTFQRNLFSEETIKSFKELYRFDDEIVL
                     QAQQTIRDFYINPTAISTLAEAINSTYPINEQSTYKRHDEFVKRIENDFPHPFKKLIR
                     WSNGIAAGADIQIFVTNYINENDLHIQNSLIVAIVCLLSFLSTYCSHSKK"
     gene            complement(12744..12884)
                     /locus_tag="NBI42_001153"
     CDS             complement(12744..12884)
                     /locus_tag="NBI42_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777581.1"
                     /translation="MLQKFRIAKEKNKLKLKLLKHASYCLERSNNPELLRAVAELLKK
                     VN"
     gene            complement(12915..13109)
                     /locus_tag="NBI42_001154"
     CDS             complement(12915..13109)
                     /locus_tag="NBI42_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777582.1"
                     /translation="MEAQNKKVIYYYYDEANNRRLLSIGNLDTYLLADIKSRFGLYKK
                     AIPDLDNLYIQIDGIEFKLY"
     gene            complement(13126..13878)
                     /locus_tag="NBI42_001155"
     CDS             complement(13126..13878)
                     /locus_tag="NBI42_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCD1777583.1"
                     /translation="MQALQTKSNIGEMFNIQEKENGEIAISGRELHQALEVKTPYKKW
                     FERMSDYGFEENIDYVVTDIFVHNPLGGRQNQTDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGVNIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTT"
     gene            13935..14474
                     /locus_tag="NBI42_001156"
     CDS             13935..14474
                     /locus_tag="NBI42_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777584.1"
                     /translation="MDIIAICIAIFSFLLTALKYYLDYMKDSLNIDVIPTRSFNYLVD
                     DKSSYNDITFINFTKFPISVIDVEFDIKNKVNEQKTFKPIRYKDKNYSIPFTLGPYES
                     VECTFLLEEYPVIWEWDVTIKVTTNKGIYIKPVIIESRTEHRESEPQVTELTSANKVS
                     ALSNPKDGFLKKFLYHLKP"
     gene            complement(14498..14758)
                     /locus_tag="NBI42_001157"
     CDS             complement(14498..14758)
                     /locus_tag="NBI42_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1777585.1"
                     /translation="MEQITLTKEECVEQCINKDLKLLDYRVQQILEGVLSESTTYGDA
                     RNKLETLKIIAESHFKTEHASVIYKLALKKLDEKINATPIKE"
     gene            complement(14771..15010)
                     /locus_tag="NBI42_001158"
     CDS             complement(14771..15010)
                     /locus_tag="NBI42_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1777586.1"
                     /translation="MHKDLYSSRKAAKKNQDFMGSLIGVSGQQYGKRERGEIPINLDE
                     AMIFSKALETPIQELFPEYFFIERVPKMHKNEITS"
     gene            15181..15888
                     /locus_tag="NBI42_001159"
     CDS             15181..15888
                     /locus_tag="NBI42_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1777587.1"
                     /translation="MSSFSSNLERLMNKRDMSDSELAELVDVNRTTVTRWRKGIRSPK
                     LDKLPEIANVFGVKPLDLIHDMDDSKIIEEIHNVSSQLTPPRQSNVLKYATNQLEEQN
                     NDSDNLVDFNSYIQEKSEVDIYGCASAGIGERLYNEPISKEFVRGYVPAHDIALKVNG
                     DSMEPLFKNGQIIFIEKSHTIKDGQIGVFIINGDAYVKKVYVEDNRLTLVSLNKKYKD
                     LHFYDNESVRLVGKVIL"
     gene            15900..16055
                     /locus_tag="NBI42_001160"
     CDS             15900..16055
                     /locus_tag="NBI42_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777588.1"
                     /translation="MNLKEVDINIEEWEMVEIPFYTEEELTYRLKNDLPITKSEFEEQ
                     ESKNEFL"
     gene            16042..16227
                     /locus_tag="NBI42_001161"
     CDS             16042..16227
                     /locus_tag="NBI42_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777589.1"
                     /translation="MNSYKEIEHLHINTGGKELTQEQIEEAKSFIDSQGFKDMIREAK
                     ESRQRVMESKITDRTKM"
     gene            16427..16594
                     /locus_tag="NBI42_001162"
     CDS             16427..16594
                     /locus_tag="NBI42_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777590.1"
                     /translation="MKITNCKIKKETIVYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLN"
     gene            16734..16964
                     /locus_tag="NBI42_001163"
     CDS             16734..16964
                     /locus_tag="NBI42_001163"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD1777591.1"
                     /translation="MEEKRYKVYQRGEIIYADFGKGVGHEFSYKHFCIVINKKDNKNN
                     GKVTVIPLTSKSKHNAFVNIHLIKPFLNTLKQ"
     gene            17446..18483
                     /locus_tag="NBI42_001164"
     CDS             17446..18483
                     /locus_tag="NBI42_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1777592.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSAC
                     RVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            18534..19364
                     /gene="sph"
                     /locus_tag="NBI42_001165"
                     /pseudo
     CDS             18534..19364
                     /gene="sph"
                     /locus_tag="NBI42_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(19637..20653)
                     /gene="lukG"
                     /locus_tag="NBI42_001166"
     CDS             complement(19637..20653)
                     /gene="lukG"
                     /locus_tag="NBI42_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HCD1777593.1"
                     /translation="MIKPLFKNITICSLAISTALTVFPVTSHAKINSEIKQVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILEPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSNKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKNKMPVTVSEGFNPEFLAVMSHDKKDKGKSKF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHDVKFVKVLND
                     NEKK"
     gene            complement(20675..21730)
                     /gene="lukH"
                     /locus_tag="NBI42_001167"
     CDS             complement(20675..21730)
                     /gene="lukH"
                     /locus_tag="NBI42_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HCD1777594.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKEKRNVTNKDKNSPVPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
BASE COUNT         6470 a         3811 c         3197 g         8313 t
ORIGIN      
        1 tcaattcttt ctttatttaa aattatcttt ccttgtttcc acaaatttat atattcatca
       61 acgtatttat tactaatcat aggtaatcat cagatggcgt ttctgtgtct tctttctctt
      121 cgggcaataa atccgataat tgtttgatta ttttttgata tgcagcatct ctagcattaa
      181 atagtttggc tactggtctt tccctttcat atggtggcgc cttttcagat tgagtaaata
      241 aatcatagtc acctttttct tttatgtctt cccacatgta atcaagcatt acacgtagcc
      301 ttgctgcttg aataattaaa ccatcaacta cttttaattt attgctaggt atgtctttat
      361 ataatacttg cagcctttct ttttctttaa gcactaagtt ttcatcaact ataatctcca
      421 tttcatcacc tgccttaaaa tggttataag agggggggtt atacatggat ttttaaaatt
      481 atcgcgaagt cgagcccctg cccgttcccc aagtattttg atcgcttttg atttttttga
      541 cccgggggta tttaccattt ttcgtctttc catttatttt ctttttttat aaatctcttt
      601 tcttttttgt tgtgacattt aatacacagt gtttctaaat tgtttaagtc atgagcaaac
      661 tccggatgat gttctagcga taatatatga tctacatcca acgacttacg cttgcttttg
      721 tcatatgtcg ttaacttgcc gtctctctta cattgttgac attcataatt atctctttct
      781 agcactcttt ttcttgttat ttgccattct ttagacttat agaatcgtat acgttcgtct
      841 ttagtcatca taatgtttca ccttatataa cttaagtagt atcaagacgc atctatactt
      901 gatgtgtagt aatgtattta caattagttt gaacatgttc atacctcata aataaaaaga
      961 cacatcacat agtaatgcgc ctcttgttca tgcgtcgtat tagcatttaa taactttaaa
     1021 tattaatctg atactaacat aataaactgt tttaatgcgg acttacatag ggtaaaagtc
     1081 cgctacacat aaccaatata ctttgctaac ttatcgatca gtgcattcct tctacgtaat
     1141 atacttgtct tacttgtacc aaagtaatgt gctatatctt cccattcata acaaccaata
     1201 ggacaatccc aatatctaaa ccttaataac tcaagcgtat cctcatcact ttcatctatc
     1261 aatctatcta caccgttaac tatatttctt aatgtattgt acctgttatc actaaacttc
     1321 tttattgcac atcgttcaat cgggttaccc ggcaaattac ttttgccagc tcccgcatta
     1381 tctggttcat gactttcaag taattcatat tctcgcatct tcaactctct tcgatagtta
     1441 tcgatgtgct gaatgtattc ttcaagcttt ttgatatcgt gtttctcaat ctttatcatt
     1501 caatgcaata cctccgataa tataaattac tttttaatat cgttattcat tcgcttcaat
     1561 tcaatcctgt attcttctaa cccgttgtat cctttagttt taactacttc atcaagtaga
     1621 taatcattca tatatctgag tgcttgtatc tctcttgcac gatcactatt aatactgata
     1681 caaactaata gcaatatagc aaatacaata gtcatagtaa tccacatcat ttaaatctcc
     1741 tcttgtttaa attaataata attctttctt ttatcgaagt tctgtttatt ttaaactttt
     1801 gactaaactg ttcttcaaat ttatcaagat aaagattata atcgtttatt tttcgtcggt
     1861 actctgaagt gataaaacta tcaatatgat tataagctct attttcattc attttattaa
     1921 ttatattttt taaataagat atatcttttt gatattcatt tataatagca attgtttcca
     1981 taacaaaaga aggattaaaa aaaatctgat ttgtactaaa ttcattgttg aaactcatct
     2041 taaaatcttc cattgcctta actctatttg ataaattaat catttgtcta aatccaataa
     2101 cattttcata cgctttttta ctttgcttat ctaaattatt aaagacttca atatcaaaaa
     2161 aatctaaaaa ttcatgctgt tctgttggaa taatataagc tccaatcatc tttttagcct
     2221 ttttaaaaca atctaaatat atcggatata tttgttctaa attgattttt ttacgttgta
     2281 agttagaatc tgtaaaatac ctaaaaatct ctttaactga ataaagcaca acacctcctg
     2341 ctaaagtata tatgcttcct attatttgct cgttcatttt tatctacctc tttataatat
     2401 tttctgaaaa ggaatctata attttataca taccaaaaat tcctaatgca tttattatca
     2461 ctccatcatc aataatatat atggatatta agaaagcaaa cagcaaaacg atcaaatcat
     2521 aaataaatat tctcattatt cactcacctc cgctcgaaag acgtaatcac tcggcgcctc
     2581 tacatcatca ttagccgtca tcataatata tacttgctca gttacatact tacctaactc
     2641 gtacattgct agtaggaata ataatcttag tatttgctta atcatttccc acactccctt
     2701 atattttcaa acaactgacc cactttaata actgcacctc ttttaacttg cgcctcgtac
     2761 ctctctttcg cttcttcttt actctctgcc tcaacaactg taaacctttg attgctctta
     2821 gctttagtta tgtgtgtatg cttgcgtcct gttgaatctt tgaatgttgt gactaggtat
     2881 tgtgtcactt ccccaaaacc tccttgactc gatctaatat gtctttacac gtatcctttt
     2941 cctgcgtctg ctgttccatc ttgtctttca tgattccttt tcattttctt tttgtacgcg
     3001 tcaatgagtt ggtcgataga atataagttg taagctatgt ctatcactat tacaattgcc
     3061 aattggtcgg gataaaattc tttgaatatt atctgtggtg tactaacaac tgcgtcttga
     3121 gcaaattctt tatctttaaa attaaacatt ttgtgaaatt ctgtattttt aaaacttgat
     3181 tcaatcgctt cttttatctc ttctgatgac actcctactt gattcgcaat actcaatcca
     3241 aacgccaaca tgtcagctaa ttcatcaagt tgtacgtcta acggcttacc tggtttcttc
     3301 ttccagttct taaacgtttc caatgtatta aaccattcaa agaattcaac tacatatgct
     3361 attttgctat ctcgtaagtc cagcgttggt attctatcgt cgaactcctt ttgtatttgt
     3421 aaaagttctt ttaattgatc tactgttaaa ttattcattt attcgttatc tcctatcgtt
     3481 ttaattcctc aataaattta agcactctat caatatcaat ctgttcactt tctgacttgc
     3541 gtttattcaa ccaataatct aactcgtacc accagtcgtc gtttaaatac ttttcttcta
     3601 gcaatgcatc acgttggtcg atgatttcaa gcatttactc gtcctccaaa aattcaaaat
     3661 atctttcaat ctcatgctcg ttagcaaagt aatatctagg cgtcttatga gatttaggat
     3721 taatccaaat aatgtaaata ggtatccttt gaaatgataa aaatccagtt aataacactt
     3781 cttcattaaa aatttcaaag aaacctaatt ctttttgaac ctctttcaca ttatcttttg
     3841 ttacgtatac cgactttaag tttttatttt tgataactgg gtgcggcacc acatttttta
     3901 atcgtttcca ttccactcac tcgtccccct taattagata aattggttta gtaataaaat
     3961 ctataatgct aataactgaa tcatcggaca gtttataatg tgtatctcta atatctccga
     4021 ccaattgcac aatctctaga ctttcgtttg tttcatggtt atatacttta tctcctacac
     4081 taatactcat tttcctgttc ctcctcatat ttatagacaa cttgacctgc cataatccct
     4141 actgcttcat caagttcaac accttcttta actgaatgtt gaatagcatt tgtcattcca
     4201 tcaagtattt catcaaacgc ttgtgctttc ttatacacgt cctcaatctc ttttagcaat
     4261 ccctctgtgt cattaccgtt atacgcacta gtacttataa cggactgttc gatttgttcg
     4321 cggttattca ttagtgtctt cctccatttg acctaaaaat tcgtagaact catttgttcc
     4381 gtctagttct tccattcgcg acagtataat atctgcagtg cttttacctc ctatatagag
     4441 agctcctatc ctgttcgctt tgctctcagg gtgtagttct ctaattttaa aacagtaatg
     4501 ttcgtatctt ccaagcaatt catttttgac tgtgcgccac atgttctcca gctcttcgtt
     4561 acgctctctt aacttagcta tatccacgat aagctcatca cgttgcttct tgcacgcatc
     4621 acgttgtttt ctcatcttct tcaacctagc ttccattaca cctagttgga accctgtttc
     4681 atagttcact ttcataacct cctctaaaat aaagttagtt ccttctgttc ttcgtattcc
     4741 aaaccatgtt gctttatata tatttcgagc tcttcagcag tatcaaatgt ctttttaacg
     4801 ccttgccaac ctggtacgat atgcccgtga aagtaataag tgccgtttac tacatggata
     4861 tgtgccactc gttcgttatc ctgatacaga tatctcttag atccaaagaa ttgatttagg
     4921 tattctttgc gtgcgttatc tgtcatgatc tacttcttaa ctttcacgaa tatgtcgttt
     4981 tccatcaggt agcacgcata acgtcctctt ggatgtttct gaggtacatt aaacaagtgt
     5041 ggcttctttc ttcttagctc agcctctctc tttcgctttc tttccaattt gcgttcgagt
     5101 ctagcttgtt ccagtctttc tattgttttc ttttctctgt actcgcttaa acgcgtacct
     5161 tctggtgcgt ccattgcttc atgtagttcc caaccgtctt ttactctctt agaaaccatt
     5221 ccagcggtta taccgtgact ttctattaat tccatttcaa atttactgaa cctataaggt
     5281 ttatcattta ttgttacaat ccttgctttt ctcgccattt tatccacctc ttatatttct
     5341 tctattcgta tgattatttt gggctcaatt ccataacgct ttgagctagt tatttctgta
     5401 atttggttat cgtctttcca tacatggcca ttacaagcat ctaataccgt tttaattaag
     5461 ttgtcgatat ccggcttagt cacttttaat tgtccaatcg cttgagcttt ctttttcttc
     5521 gaccatgatt caggtggagt aaagtaaaac gctaattcga tttttaatgc attttctaga
     5581 tttagccttg gcatttgact ttgtaaatat tttttatgtt ttgtatagtt tgtaggcatg
     5641 tatgtgtgta cgtatctgcc ttttgtgcta aaacgcggtc gaggcgaccc catcggcgca
     5701 ttaaacactt cgttaaattt aatttctatc tccatgcaat ccctcatata tattcaaata
     5761 agtttgtttg gtgtcctaac tccatttgtt cattatcaat aagtgtattt aattcataat
     5821 cgtctaaata ccaacgacga ccattaaatt ttgtttcttt tattccaaca actaaatgcc
     5881 gaccatcttt aaaatgtggt gtaactgaaa acattttgtt gccgtcatga tcaaatagat
     5941 agtatttatc aaatgcatcc attttcaatc actcccattt gctatttaga cgcttaataa
     6001 aagcttctct gtctttctca aggttttcat ctacttccgc cgttttcgtt tctctcgtgc
     6061 cgtctgtgag ccatttaggc gttttttctt ttgattgttt aacatgaggt ttgtatgttt
     6121 gctttttgct ttcaatttgt tgcttttcaa atgcacgtac ttgttcgata gatttcaagt
     6181 ttgcattaag ccatgtattc aaaatgcttt tagcatatcc ccaagtaact ttgtttctgt
     6241 ctttagcgat tttaagtgat gccgtaacta tttcatctga atcattttca aatgaatcaa
     6301 gataataatt taaatcgtct aaattgtatg gagttatgaa accgaatccg ttatcttgga
     6361 agaagtcgaa ggcagctgcc ttcttcttct cattattcac attcttttca ttattatctt
     6421 tattatcatt attgtttgtg ttggtttgat gttgttttga tgttggattg atgtttgact
     6481 gatgttgttt tgatgttggt ttgatgtcgt tttgatgttg gttccttccc tgctcacttt
     6541 gataaaagtc ataattgaca atggttataa gggtatattt tgatgttgtt ttgacttcta
     6601 acattccatc actctcgagt aagtcaagga aggttttcac tttaaatcgt gaccagttaa
     6661 aaaggtcaga caaggtcaaa atcgatgtta atctttgtcc tctttctacg gttacaattt
     6721 ggtttccaat aggcactttt gcctttgaat gattcgcttc catgagtaaa tatatccatg
     6781 cttcaaactt tgaaaatgtt ctcttttctt taaatagcca atgattttga attgagcgat
     6841 caatacttat ccaaccagtc atatacacac ctcactttca aaccggttaa attagaatgg
     6901 taaatcattg tcatctattt caatcggaca atttgcattc gcaaacggat tatcttttac
     6961 tggtttgtta tttgaatatt gcgattgtcc acgtgtttgt tgtacttgtt gttggtataa
     7021 atcttgttga gtgtcatttg agtttttcgg ttctaaaaat tgaatactat cggcaacaac
     7081 ttccgtaacg tatacacgtt gaccttcctt attttcatag ttccgcgttt gtaacctacc
     7141 atctacgccc gccaacgatc ctttagatag gtatttatta acgttctctg cttgtttttt
     7201 aaatacgatg atattaataa agtctgcctc gcgctctcct tgtgcattcg taaatgtgcg
     7261 gtttactgct aatgtgaatg atgctacatt tacaccactt tgagtggttc ttaattctgg
     7321 gtctctagtt aaacgaccaa ctaatattgt tctgtttagc atttataaac ctccaacata
     7381 aacgggcacg cccgtcactt tttgtatttc tcttttaatg tattttgcat ttgaattttg
     7441 gctacttaaa tgaattaaat gtatttcttc gagtctagtt aaatcatttg ctttcaacat
     7501 tcctatagca tgttctaagc taaaatgaga ctccataatt ctatttgcta atgcgctgtg
     7561 tacactgccg ttttttatgt tttcttgcat ttgttcatag atataattaa cttctaacat
     7621 catgtgtgta atgccgttaa atttgtattt cagatacttc gtatcagtaa catacaggat
     7681 cttataacct agtgtgcttt gtaataagaa agccacaggc tcgttagcat catgttcgat
     7741 gtcaaacggt agaattgacc atgtacctat tcgcagctct tgctttgcct taatcgtgca
     7801 taagcgatga ctttcaaaat tcatagcttg ttgtgttcca gcagtcatat agctgattac
     7861 accattgtcg acaaactgct ttgtatactt tgcatgatca ccatgttcgt gtgtgataag
     7921 acaccctgct atatgtcttg ttttatattt gaaatgcttt tgaacacgtt caaattttat
     7981 acctgcctca agtagtaacg tagtacgtcc atcatttaag acgtagcagt taccacttga
     8041 accagttgct attgtttcaa ttaaaatggc tcttcttcgc tttctttttc tgttgcaggt
     8101 tcttttattt cttcaaagtc agatacatca ataggcttat cattttctaa ttctgtgtat
     8161 tgtgcttctt caagaactgg ttgttcaaag tccaattgtt cttgatttgc attttcttca
     8221 acttctgcgt ccaacacttc tttgcgttga cgttgttcgg attcttgtgc gtatttgaaa
     8281 agattgctat ctgttgatgt gttgatataa cgtttagcag ctctattgat aactgttttt
     8341 ttagccattt cttctttgaa attattatgt gttttagaat tttgtaatgc tttttcatct
     8401 ttaatcattg atgactgcat ccatgcttgt ttaatttgtt caatagtcat gacttcaata
     8461 tagttatctc gtccatcatt aaatacgatt gtgcagtacg caccgataat gttttctttg
     8521 tcgatgttaa agaagtcttg ttcgtgctta atcgctttga tacgtcctgt ttctcccatt
     8581 tcttgcttga atgtatcgcc tttataaatc acttgagcaa caacatcttg agcacctgca
     8641 tcacgtttta acatcattac attaccgtga tagctacgtt gtaactgcat tttgttgccg
     8701 taaggaataa agtagcattg atttttagct ggatttaaac cttgcgttac catgtctaat
     8761 aaggcatttg ctttgcttgt atcgttacaa ctcattaatt tgttatcttg gctgatttgt
     8821 aaccatgctt gtttcatggc attacttggt gaataatcat ttggcaattc caaattgcct
     8881 tgtgactcta aaactctcac tttgtttaat acgttgtcag atacgttctt ttcttgtact
     8941 aattgttgtt caatagtttg taatttatta ttttcagtca ttttatatag tctccattct
     9001 taatttttta tcttgttcat ttactatcaa ttgaatttgt tgtgattctg ttttgataag
     9061 ctctgttact gattcagcat tatcaataaa tattggcgct gtaactttaa aatgttttga
     9121 cagtgtattg atgatatcta agccaacatt aattcttgag gcgttattta aaccgctgtc
     9181 gtattcgacg ccgttaaccg ttgttgaaca tgtttcttct aattcgccgt taactaaggt
     9241 attgaatagc ttaaattcag caatatcaaa ttcgttattg atattttcag taagcatttt
     9301 gacttttgtt gttgtaaatt cttttaagat ataaaggtta tgtgaatact tttctttttc
     9361 atccaataat ctgtcttctt catttcttaa ttcagaaata acatcatcta gatgtttatt
     9421 tgatttttcg attgatcttg acacttcaat ttctgatttt tcttgagtaa gttcgcttat
     9481 tttgtcatct attcctgaaa ctttatcttg aatagttttc ctgatgtttg agcgtttttg
     9541 attaatttca tttatctcta acattactgc tttgtattcg tcagtttgtg taacgtcaac
     9601 gtgagtcgtt tttaacttat taattttgtt ttgtattctt gctgaacgct cttctgcttc
     9661 gttgatttta atttgaagat tattattgtc atcctctaac ttctcgatga ttggctttat
     9721 tttcttgcct tctgaaataa tgtgattgat agatgtttgt attgtttcta attctttcga
     9781 tttgctagca ttgaatttct gcaatgcttt ttctcttgcc tcattcactt gttcagctgg
     9841 taactgttga ccacaacaac tacatacatt gtcatcaaga tattcaaatt tttgattttt
     9901 agctttttct aaatcacttt ttaatccttt atgattttct aataattgat tacgtctatt
     9961 ttcttcatgt gcgatttgtt gtttgttttg atttaatctt gttttaaggt tcgcaaccgt
    10021 tccattttca acgtgtaact catttgttaa agtatggatt ttgttctcat tacttgcgct
    10081 gttattgtct tcaatgcgct tcaattctga ttgtttatca gctaattgat tacgcaaatt
    10141 aatttcttct gcaccgtttt gaatatctat acgctcattt tcaagttgct caatttcttg
    10201 tttgataatt gcgtatctat cattatcgaa ttcaggtaca tcctgcttat tttgttgtgt
    10261 ttggttaata cgtatcggaa tatctttgat atctttgtta atctgtttta tcttgtccgt
    10321 aagaatcttt ttctttgttt caatttcatg atctccaaga atattattta attctttaaa
    10381 atcatcattt gttttaatga catcctcatc attgattggt ttagcaattt caaacaacaa
    10441 acttcttcgc ttcttccaat ctagtaagtt aaatgcttga gggttcgtaa ttaacttgaa
    10501 tacatcttca tcaatcagtt catcaatacg agctttataa tcctttactt ttattgattc
    10561 atcattgata tattgtttct tcgttcgact tcgtgagtat tccttgcgat tcgttttttg
    10621 atttattgtg tatttaggat gtgactcttt tttgaaagtc gttatttttc cgtcgatttc
    10681 aaattctgcg aaaacagtcg gaattaactc ataattttct tcgttttttt cgtttaaagg
    10741 tacagggtta aatgatttgg ttgatccgtc caaaccttta tcgaaaagca gccattgtaa
    10801 tgcggttgca gtcgtagtct taccagtcgc attattgccg tatatttttg tgtctttacc
    10861 gtcaaagtta aagctttctt ctttgattcc agcaaagttt gatattgtta gcttatttat
    10921 ttttatatcc atcatcatgc tcctttttta atctttcggt gacctcttaa cacctcgata
    10981 attaaatttt ttattcgttc gtggctgtcc ggattgattt catgtatctg tacaagctta
    11041 ttgttcgttt tgtaactgtc gtgatagtgc aagaaattaa tcgataagta tccgtgatga
    11101 ttacgttcaa tttccaataa tgctcgttgg tttgacaaag tatattcgtc gaataacgtt
    11161 ttaaaaatat tcaatatatt tctttctgta tctctcatgc ttatacctac catctcatga
    11221 ctaagttaat tagcctgtct ctttcgtctg tgttctcttc aatccattca taaatagatt
    11281 gatttaatat gtctaatgct gtgtatagat cattctcatc tgttatattt atactgtcga
    11341 taaatctatc ttctaaatct aagacattca ctagaatgct gtaatcttgt ttcttaactg
    11401 ctaatttaaa atcgaatccg tctacattaa ttactttttg acatacatcg ccaattttgt
    11461 agtacattgt tgacacttcc tttatttcgt tttatattga atatgcatta attttctaat
    11521 tgtttagact gttactcatt gccgtgagta acagtttttt tattcttcat aaaagtattc
    11581 cttataaaat atgaatgttg cgatacttgc gaatcccgca atcgaccatg cagtagtgaa
    11641 gtatagaaac ggcataagta caattgctaa gactgtaaag cacagtactg ctactaggta
    11701 gcttttataa atgttgctca ttttattctc tccttatata tttcattgaa atgctcatcg
    11761 acgaatttat tcatctttct tgcgttaaat ctccagcgat taaaattctc atctgggtaa
    11821 tgcacaattc cttgcgctct tagttctttt tcaaatctag gatgaaatag taatctgtcc
    11881 ttgatagtct catcagatgc aatttttaat ttcttcttta agtcactcat gttccataca
    11941 gggtctaatg aataaccaat tagctcatca tattcatctt ttgtgataag tacatgtgtt
    12001 tcaggtattg gaactgttac gtttaaaata tgtggcattt ctatctttcc tttcgtgtat
    12061 aatgttgtta tctcctagtg aaaggaggtg atattggtgt atattgatcc tttaaaaaat
    12121 gttcgttttt ctattaataa cgtaattagt aatgttgaaa tttctaaaag tatggcaatt
    12181 aaacaatctt taaaacctaa gtaccaatta gatataatta atagaaacaa cataaattta
    12241 ttttctgact tcaaagtaga ctttcatcta aacaacttaa ttgaaatgaa ttttaatttg
    12301 cgtaattctt tttcatctct aacatttcaa agaaatttat tttctgaaga aacgataaaa
    12361 tcttttaagg aactctatag gtttgatgat gagattgtac ttcaagcaca acagaccatt
    12421 agagattttt atatcaatcc aactgctatc tctactttgg ctgaagccat caattcgacc
    12481 tatccaataa atgagcaaag tacctacaag agacacgatg aatttgtcaa acgtatcgaa
    12541 aatgattttc cacatccttt caaaaagtta ataagatggt ctaatggcat tgcagcaggt
    12601 gctgacattc aaatctttgt aacaaactat ataaacgaga acgatttaca tattcaaaat
    12661 tcattgatag ttgctatagt ttgtttatta agttttttat cgacctattg ttcacattct
    12721 aaaaagtaat aataaggcct aatttagtta accttcttta acaactctgc aactgctcgc
    12781 aacaattcag ggttgttact tctttctaaa cagtaactag catgctttag taatttgagt
    12841 tttaatttat ttttttcttt cgcgattcta aatttttgta acatttgtta tgcctccttt
    12901 gcatttccaa aaatttaata taatttaaat tcgataccat ctatttgaat gtatagatta
    12961 tctaaatcag ggattgcctt tttatataaa ccaaatcttg atttgatatc tgctaataaa
    13021 taggtatcta agttaccaat tgataatagt cgtctattat ttgcttcgtc atagtagtaa
    13081 tagatgactt ttttgttttg agcttccatt tgctgtgccc tcctgttaag ttgtttgttt
    13141 ttctcctaaa aacttattaa caaagtattg ttgtcctttg cctgttactt ttggcgtctt
    13201 actaattgat gtgtgaccgt ccgaatgtgt gattgatgtt tctttaattt cgaataactc
    13261 acgttccatt gaatactgtg taggcatgtt ataatccaca cccttgcgtt taataaggaa
    13321 tccgttttga cgtaaccact caaacaatct gcgttgcccg atgtttacac cgttttgttt
    13381 aatgatcttt gctaactctc caactaaaat tgatgtctta gtagtagcta ctgcatctgc
    13441 aaatacaatt tttggtttat cacgttcaat ctttgtttct aattgattga ttgtgttgtt
    13501 agcaattttt aaagcacgtt gcataatcat ttctgggctg ttccatgctt tttcaacttg
    13561 gatgaagtat tgtcttgcac gtttaccagg ttcactgcgt tgaatcattg cgatttcttt
    13621 tgcagtgtct agtgtgagtg cgtggtcagt ttgattctga cgacctccta gtgggttatg
    13681 gacaaaaatg tccgtgacta cataatcgat attttcttca aatccgtaat cactcattct
    13741 ttcaaaccat tttttgtatg gagtcttaac ctctaatgct tgatgaagtt ctcgaccgct
    13801 gattgcgatt tctccgtttt ctttttcttg tatgttgaac atttcgccga tgttcgattt
    13861 tgtttgtaat gcttgcatat tgtttatgct cctttcgtgt ataatttagt tatcaaccta
    13921 aggaggtgat aagtatggac ataatcgcga tttgtatcgc aatttttagt ttcttactga
    13981 ctgcacttaa atattattta gactatatga aagattctct taacatcgat gttataccta
    14041 ccagaagctt taattacttg gtcgatgaca aatcaagtta caacgatata acatttatta
    14101 atttcacaaa gtttcccatt tctgttattg acgttgaatt tgatattaaa aataaagtaa
    14161 atgaacaaaa aacgttcaaa cctatacgat ataaagataa aaactactcc attccattta
    14221 ctttaggacc ttatgaaagt gtagaatgta cttttttgct cgaagaatat ccagtgatat
    14281 gggaatggga tgtgactatc aaagtcacta ccaacaaagg aatctatata aagcctgtta
    14341 tcatagaatc gcggacagaa caccgagaat cagagccaca agtgacagag ttaacatcag
    14401 caaataaggt aagtgctctt tccaacccca aggatggttt tttaaagaag tttttatatc
    14461 atttaaaacc ttaaacattt aaaatcctcc ctttccgtca ctctttaatt ggagtggcgt
    14521 tgattttttc gtctaacttt ttcaatgcta atttgtaaat aactgaagca tgttcggttt
    14581 taaaatgaga ttcagcaata attttcaatg tttctaattt atttcttgca tcaccgtatg
    14641 tggtactttc tgatagaaca ccttctaaaa tttgttgaac tcgataatct aaaagtttta
    14701 agtctttatt gatgcattgt tcgacacact cttctttggt taacgtgatt tgttccatag
    14761 tgtcctcctt ttaagatgtt atttcgtttt tgtgcatttt tggaactcgc tcaataaaaa
    14821 aatattctgg aaatagttct tgtataggtg tttcgagagc cttagaaaaa atcatcgctt
    14881 catctaaatt aataggaatc tctccgcgtt ctctttttcc gtattgttga cccgaaacac
    14941 caatcaaact ccccataaag tcttggttct ttttcgccgc ttttctagag ctatataaat
    15001 ctttgtgcat ttttggaaca cctcctgaaa acaatactac acctgatgtt ccaaaattgc
    15061 aagtgctttt tacatatttt tttgcctcta cacatatttt tatgtttttg ttgcattttt
    15121 ggaactgtag gcatataatg aagttattag ttagataata tgtttaaagg agataaaaat
    15181 atgagttcat tttcttcgaa tttagaacgt ctgatgaaca aaagagatat gagtgatagt
    15241 gaattagcag aattagtaga tgttaataga acaacagtca caagatggag aaagggaatt
    15301 agaagtccaa aactagataa attacctgaa atagctaatg tttttggagt taaaccctta
    15361 gatttaatac atgatatgga tgattcgaaa attattgaag aaattcataa cgtatcatct
    15421 caactcacgc ctccaagaca aagcaatgta ctaaaatatg cgactaatca attagaagag
    15481 caaaataatg acagtgataa tctggtagat ttcaattctt acattcaaga aaaatccgaa
    15541 gtggatatat atggttgtgc gtctgctggt attggtgaaa gattatataa cgagcctatt
    15601 tcaaaagaat tcgtaagagg ttatgtcccc gcacatgata tagctttaaa agtaaatgga
    15661 gactcaatgg agccgttatt taaaaatgga caaattatat ttattgaaaa atctcacact
    15721 atcaaagatg gacaaatagg cgtctttatt ataaatggag atgcttacgt aaagaaggtt
    15781 tatgtagaag ataacagatt aacgttggtt tctttaaata aaaagtataa agatttacat
    15841 ttttatgata atgaaagtgt gaggttagtt ggaaaagtta ttttatagga ggtagtaaaa
    15901 tgaatttaaa agaagttgac attaacattg aagagtggga aatggttgaa atcccctttt
    15961 atacagaaga agaactgact tatagattga aaaacgattt acctataact aaaagtgagt
    16021 ttgaagaaca ggagtctaaa aatgaattct tataaagaaa ttgaacactt acacattaac
    16081 accggtggta aagagttaac tcaagagcaa atagaagaag ctaaatcttt tatagacagt
    16141 caaggattta aagatatgat tcgagaagct aaagagtcac gtcaaagagt tatggagtct
    16201 aaaattactg atagaactaa aatgtgatta ataacgtata tttagcgctt taatataaat
    16261 ataaacaaag gagaaattga catgaaaaaa gcaatcttaa ctttaagtct tatatttatt
    16321 acctactacc tcacttttaa atatatgtgg attaaagaat tgaagtatta attatgctta
    16381 tttaaaaaag acgtctattt cagcagtgtt tgaaaggaag tttataatga aaataactaa
    16441 ttgcaaaata aaaaaagaaa ctatagtata tgaagtttta actagtggta atcaaccatt
    16501 cacttatgag ttacctaaag atttatcgtc acataatgcg cgtaaatact tggaatttat
    16561 ttcacaaaaa atagatggcg ataagttaaa ttaattcaaa gaataaagta acttcataaa
    16621 gagtacgaag aaaacgatct aatgaccgaa cttattcttg aatatttagt aaaaaagtat
    16681 gttgaagaag aatataggaa ataaacgcct atatggcgtg aggaggatga gggatggaag
    16741 agaaacgata taaagtttat caaagaggcg aaataattta tgcagatttt ggtaaaggtg
    16801 taggtcatga gttttcatac aaacactttt gcatagtcat aaacaaaaag gataataaaa
    16861 ataacggcaa agtgaccgta atccctttaa cttccaaaag caaacacaat gcttttgtaa
    16921 atatacatct tataaaacct tttttaaata cactaaaaca ataaattgcc aaacacgaga
    16981 aaaagataga atcgattgaa aacattataa atcaaaatcc caatgattta aaagtcattg
    17041 tttatgcaag gtttttgtgt gactccttaa agaacgaaat gaaacaatac gattttgtgg
    17101 aaaacaaatt aaaaaactta gttgacaaaa gttcttatgc aaaaacacag gatattacta
    17161 caataagtaa ggatagaatt ttaaattata ttaaagattt aagcgtttta attagattgc
    17221 cgaaacacga aactgaccga ttggttaaca tctgtttcaa aaaaatgagt tgaacaattc
    17281 taaaataatg ttataataca ggtattgagg gctaaaggtc cacctatatt gatttgagct
    17341 ttcggctcac tgacgtctgt attatacagg cgtctttttt tatacaattt tcacgggtag
    17401 cccgcctacc cttattattt tttgccaatt ttgaggaggg aacgcatgaa aacacgttgt
    17461 tacgatggta aaaaatggca atatgaattt aagtatgaag gaaaaagata ccgtaagaaa
    17521 ggttttagaa caaagcgtga agctaattct gctggactag acaagttaaa tgagttaaga
    17581 agtggtttta atatagataa ctatataact cttgaagaat acttcgaaaa ttggattaaa
    17641 acgtataaac aacctgttgt taaagaaaat acctaccgtc attatagaaa tgcattacaa
    17701 catatacaaa aacataaaat aggtaaaatg gagttatcaa agataaatag acaagtttat
    17761 cagaaattca taaacgatta ttcaaaagaa cacgcaaaag aaactataag aaaaacaaac
    17821 ggtgctattc ggtcagcttt agatgacgca ttatatgatg ggcttatttt taaaaatccc
    17881 gcttataaag ttaattataa agccggaaaa cctacgaagt cagaacaaga aaaattcatc
    17941 tcggtaactg aatatgaaat actaaaagat cacgtcagaa agaagagaac tcgttcatca
    18001 ttagcgctat tcataatgat ttgtacgggt tgtcgtgtca gtggtgcaag aaatataaag
    18061 attgagcata tcaaccaagt gaaaaacact atatttattg acgagcgaaa aaccgatact
    18121 tcccctagat atatcagtat cgctaaatct gatatgaaac acattatgga cgtcataagt
    18181 acatttgcaa ttagctatga tggttacatt ttcaaagaag ccggatctat aattaacctt
    18241 catgctatca ataatgcttt gaaatcagcc tgtagagtca ataatatacc aattattaca
    18301 tcgcacgcat taagacacac tcattgttct tatttactag caaaaggtgt atctatacat
    18361 tacatttcta aaagattagg tcataaaaat atagcaataa ctacatccgt gtattctcat
    18421 ttgttagaag aaaaatttaa tgaagaggac aaaaaaacaa ctaaaatttt agaaagtatg
    18481 taatttaggg acccattagg gactccaaac ccaataaata ctgttgttac aaggtttcta
    18541 tgtatccaaa ctgggggcaa tataaacgcg ctgatttaat cgggcaatct tcttatatta
    18601 aaaataatga tgtcgtaata ttcaatgaag catttgataa tggcgcatca gacaaattat
    18661 taagtaatgt aaaaaaagaa tatccttatc aaacacctgt actcggccgt tctcaatcag
    18721 ggtgggacaa aactgaaggt agctactcat caactgttgc agaagatggt ggcgtagcga
    18781 ttgtaagtaa atatcctatt aaagaaaaaa tccagcatgt tttcaaaagc ggttgtggat
    18841 tcgataatga tagcaacaaa ggctttgttt atacaaaaat agagaaaaat ggtaagaacg
    18901 ttcacgttat cggtacacat acacaatctg aagattcacg ttgtggtgct ggacatgatc
    18961 gaaaaattag agctgaacaa atgaaagaaa tcagtgactt tgttaaaaag aaaaatatcc
    19021 ctaaagatga aacggtatat ataggtggcg accttaatgt taataaaggc actccagagt
    19081 tcaaagatat gcttaaaaac ttgaatgtaa atgatgttct atatgcaggt cataatagca
    19141 catgggaccc tcaatcaaat tctattgcta aatataatta tcctaatggc aaaccagaac
    19201 atttagacta tatatttaca gataaagatc ataaacaacc aaaacaatta gtcaatgaag
    19261 ttgtgactga aaaaccaaag ccatgggatg tatatgcgtt cccatattac tacgtttaca
    19321 atgatttttc agatcattac ccaatcaaag cctatagtaa atagtgctca actaaatcta
    19381 tcgcttcgtt caataagttc gaagcgattt tttatgtctt taagttaaat taaactcgtt
    19441 agttcaaatt tatgtaaaat aattataaat atatataaac cttatcaatg gccttatctt
    19501 gttctagttt cttcattaat ataatagatt cttcattttc aaatcatatg cttgttccaa
    19561 tataatctat attcctttta aaattagata aaagaaacct gctacgtttt tgttcgcagc
    19621 aggcaacttt tattacttat ttcttttcat tatcattaag tacttttaca aacttcacat
    19681 catgtgtctt ccaatcaact tcatataatg ctgataattt ttcttctttt ttatctacat
    19741 ggttttcacc agaccaatag ccccagaaac catgacgatt ccaatctatt ttaaactcat
    19801 ccattgatct tttataatga acaacaaatt ttgatttacc tttgtctttt ttatcatgtg
    19861 acataacagc taaaaattct ggattaaacc cttcagaaac agttacaggc atcttattct
    19921 taggtgtgaa attatctttc gcccataaat ttccatttcg tgttaaagaa aagatttcac
    19981 ttttagttct attatcacta tcattagtta attgtctcgt atggtcatgt cccatattat
    20041 ttatcaaatg agcttctact ttccaaccta cacctttatt cgacgtagat tgatcaagta
    20101 atgtacgata tgaaggttgt tgataactaa tcgtctctga ataattactc tcttttgtaa
    20161 tatttccagt taagccacca cgattaattg aaaagtctcc acccgtttta taaccatacg
    20221 tatattttac ttctcttgat tcatcttgat tttttggtgc aaagtccgtc acatttgtat
    20281 tgttgttgtc atcaacgttt tgaattgaaa ctgaataaga tcctggccac cttaatgtgc
    20341 tattccaata accatttggc tctaaaattc tcaaaccact gccaatcgta ccttttgctt
    20401 taataaatac tgtttcttta tcgtaattag gttcagttaa aaaattaaac tgtaaacttt
    20461 gagtaatatt tttctgacta tcacttgttg tagctgtacg tgtatacatt ttagtatcgc
    20521 catccaggtt cttctcagaa acttgtttaa tttcagaatt aatttttgca tgagaagtta
    20581 ccggaaatac agttaatgca gtagatattg ccaaactaca aattgtaatg tttttgaata
    20641 atggtttaat catttgtaat ccttcccttc gattttatcc ttctttataa ggtttattgt
    20701 catcagaata tttatcaacg actttaactg ttttattttt ccaatcaact tcataagtga
    20761 caattaatct ttgaccatct ttattttttt ctaaaattgg aggtgcataa tgtattccag
    20821 gtctgttttt caaaatatct tgatttcgtg tgtatgttac ttcaaattgc gttttctcat
    20881 ttgacttttc attagataaa taagttaaaa attctggatt aaagccactt cttactaatg
    20941 ctgggtatct atattttgaa gcaaagctta gttcagggtt ttctacagta gcaattctcg
    21001 tatttctata gaataataat tcatcatttc tatttttcac ttctccacca tacttcaagt
    21061 cattcgcaat aactgaccag tgtacatgcc agttattatt tttaccgctg gcaattgtgt
    21121 cataattttg ctgattataa ctaatcgttt ttgagtagct atttgatgaa gttcgtccaa
    21181 taccttttgt tgaatcgaat ttaccacctg agctatatga aaatgtactg tctactttcg
    21241 cagttgaaat tttatttttc ggcaattggt ctaatatttc agttttacga tttcttttta
    21301 cttgaaaatc tacatggtac tcacttggat actttaacca atttgaattt ttttcttctt
    21361 tatgagattc aaactttaaa tttgaatgaa ttgagccttg ttttttaaca agtaatacat
    21421 tcttgtcata agttggatca tcaataaagt cgaattgtaa attttggagt atatttgttt
    21481 tctcatcata tactgtttca gttcgttttg tgattttacc gtttttacca atatcatcag
    21541 gtactggtga atttttatct ttattagtta cattacgttt ctctttttgt tgagacttat
    21601 caacatgttc tttcttattt tggtcttgcg agtctttatg agctgaattt gcttgagtcg
    21661 ttgctgctga taacaataaa attgcacatg ataatgatga cgctattaaa acacgttttt
    21721 tatttttcat tgttataacc ttctttcgta tgaatttcgt atatttaata attgttgata
    21781 cctgtcgaca t
//
LOCUS       DAHHRR010000035        21781 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.035, whole genome shotgun sequence.
ACCESSION   DAHHRR010000035 DAHHRR010000000
VERSION     DAHHRR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21781
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.035"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(90..479)
                     /locus_tag="NBI42_001168"
     CDS             complement(90..479)
                     /locus_tag="NBI42_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HCD1777595.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGGTPYFKPLHMVLRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPLSKLFGIG"
     gene            complement(806..1708)
                     /locus_tag="NBI42_001169"
     CDS             complement(806..1708)
                     /locus_tag="NBI42_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCD1777596.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            complement(1874..5527)
                     /gene="addA"
                     /locus_tag="NBI42_001170"
     CDS             complement(1874..5527)
                     /gene="addA"
                     /locus_tag="NBI42_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCD1777597.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEVQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDGVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTLDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVNYDESAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAFRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SESIPDDLKFEKDIAQIEDSSRPNVNISIIYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQFPEGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGKQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            complement(5528..9004)
                     /gene="addB"
                     /locus_tag="NBI42_001171"
     CDS             complement(5528..9004)
                     /gene="addB"
                     /locus_tag="NBI42_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCD1777598.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLESFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQKEFITGEDALQYFIDCMPKSEWLKCADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFKQSYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESGTMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVFDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGNVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLDDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKPLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWADIDEDKLEQDLIKKFKLSGLV
                     NSDQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NINIDDEFGGEQ"
     gene            complement(9164..9739)
                     /gene="lepB"
                     /locus_tag="NBI42_001172"
     CDS             complement(9164..9739)
                     /gene="lepB"
                     /locus_tag="NBI42_001172"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD1777599.1"
                     /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKYNFNPENTKN"
     gene            complement(9755..10279)
                     /gene="lepB"
                     /locus_tag="NBI42_001173"
     CDS             complement(9755..10279)
                     /gene="lepB"
                     /locus_tag="NBI42_001173"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD1777600.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDHDNNQHDSRQFGLIDKKDIIGNISL
                     RYYPFSKWTIQFKS"
     gene            complement(10284..10859)
                     /locus_tag="NBI42_001174"
     CDS             complement(10284..10859)
                     /locus_tag="NBI42_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCD1777601.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLIVFWIVGKKLEQHFMGSKKE"
     gene            complement(11172..12503)
                     /locus_tag="NBI42_001175"
     CDS             complement(11172..12503)
                     /locus_tag="NBI42_001175"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCD1777602.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            12854..14059
                     /locus_tag="NBI42_001176"
     CDS             12854..14059
                     /locus_tag="NBI42_001176"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCD1777603.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            14049..15428
                     /gene="argH"
                     /locus_tag="NBI42_001177"
     CDS             14049..15428
                     /gene="argH"
                     /locus_tag="NBI42_001177"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCD1777604.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            15670..16599
                     /locus_tag="NBI42_001178"
     CDS             15670..16599
                     /locus_tag="NBI42_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD1777605.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYTGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(16740..17984)
                     /locus_tag="NBI42_001179"
     CDS             complement(16740..17984)
                     /locus_tag="NBI42_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HCD1777606.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(18093..19283)
                     /locus_tag="NBI42_001180"
     CDS             complement(18093..19283)
                     /locus_tag="NBI42_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCD1777607.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQINHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            complement(19591..20718)
                     /locus_tag="NBI42_001181"
     CDS             complement(19591..20718)
                     /locus_tag="NBI42_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCD1777608.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDNGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(21080..21457)
                     /locus_tag="NBI42_001182"
     CDS             complement(21080..21457)
                     /locus_tag="NBI42_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCD1777609.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
BASE COUNT         7053 a         3947 c         3133 g         7648 t
ORIGIN      
        1 tgtttaaaca tttttagaat caaaaaagac ataaggtctt ggactaataa ttgtccatgc
       61 cttatgtcat atactatatg tcttatcaat tagccaatac cgaataattt tgataaaggc
      121 cctaacggta gaatgacacc taataccatt gtgatgataa ttaatgcaat tgttatccaa
      181 aacattgtgt gactttgttc atgtctcttt cttttagcaa tcgacacttc catcaatcca
      241 actactgcaa caccacacag cattttcaat gtaagcaaca tatgatttgc cccgccattc
      301 ataaatgact gaattaatat ccaaaatcct gaaattaacg tcaacagcat aaataatcgt
      361 agtaccatat gtaatggttt gaaatatggc gtaccacctt gattttttga aatatttaag
      421 tatgtagcga taaataaaat gattgctaat acccaactta aaatatgtaa atgtaacata
      481 ttaattcccc cactttaata attttagtta ttaaattaaa acttcgtggg ataaataccc
      541 actttcttat aatttaatca ttatttgttc atacacaaat acatatttta ccacacttct
      601 atatttgtga ggaagaacat gacattttat tacttattag aatattgtgc atctgctgta
      661 aaataatcaa ctactgctta atatcaagat ttcactttcg tctcaaaatg gtaacattat
      721 aaacaattat ctttaacacc tttttagaaa agcaagaaaa aactaaccaa tctatataaa
      781 gactggttag ctttttaaat gataattatt tagcgatata agttgtcagc gttccaatat
      841 tatcaatagt cactttaact tcatcacctg gttgtaagaa tttaggtggt tgcatacctg
      901 caccaacgcc tgctggtgta ccagttgcaa taatatctcc cggatgtagt gcaacatatt
      961 ttgaaatttc ttctattaat tcatcaattt taagaatcat ttcgccagtg ttaccatctt
     1021 gtctaatttc attgttaact tttgtaacaa tatttacatt ttcaggtaat ggtagttcgt
     1081 ctttagtaac gatataagga cccattgggc aaccgccagt taaacttttt gataaaaatg
     1141 cttgatcttg ttcactttgt gctttgcgat cagtgatatc gttaataatt gtatagccgt
     1201 atacataatc taaagctaat gcttttggaa tcttttcacc agacttacca ataacaatac
     1261 ctaattcacc ttcataatct aattgatcag taatatcttt atgatttgga attgttgcat
     1321 tatctccagt taaagatgac gctgcttttg taaatacata taatttttcc acttcatgat
     1381 ttaattcgtt cgcatgatct ttgtaatttc taccaaaagc aatcacatta ttcggaggtg
     1441 ttactggtgg taaaaattca atgtcattaa atgaaatttt atagtcttca gctttgccgc
     1501 tatcttctgc tgctacaact gctttacgta cttgttcttg aaaatctaaa gtatgatttt
     1561 gttgtaaacc agctaacaat gttttaggat ggaaatctcc ttctgcaaag tcagcaaata
     1621 cttgtgttaa atcccataca gcatcttcgc gttttacttt aacgccatat gaagttttgt
     1681 cattatactt gaatgataag aatttcattc attctcaact cctcatcttt atcttaattc
     1741 acattataac ttttttcgtt atcaaataac aaataaataa gtaagacaat tttgaaaatg
     1801 agttgtgttc attctgctac aaggactttg cacttaatcg aaattatttt ttattctttt
     1861 gaaaatcaaa atactatagt tgcaatgtac caaatttgaa gaagtataaa taacctttaa
     1921 cttctttatt aagaatcgtt tgaagcgtat tttgataata tttcatctgt atcttatatt
     1981 tattttttaa ttgtttacca atttcttcat ctgtcatccc acggcgacga ttaaatgcat
     2041 cggttttata gtctacaaaa taatgcacac catctttaac aaagattaag tcaatcatac
     2101 cttgaataat tgagacgtct tcgtctcctt ctgggaattg gtcaactaat gcttggttaa
     2161 ctacaaacgg taattcacga taaacttgct ctgcttcagc aataatcgaa tataactcac
     2221 tattgataaa tgtcattatt tcatccatac gaatatcttt tttcgcatct gcttcgataa
     2281 tatgtttatc gattaatcca tcgatatact gatgtaactc aacttcagat atgcgttctt
     2341 ttttgaatgg taaatgttgc atcactgtat gcattaacgt accaatttca ttcgcttttc
     2401 gtttaccttg ttcacttaga aatttaggtc gttcataagt tgaaaaaccg atacgatatt
     2461 gccttactcg ttcgtaactt gtgccacttt cttcagtttc atattgtctt ttcaattcag
     2521 aaacagattg ttttgagggc tttttagtat catttacata tggatattga taatcaagtt
     2581 ggtgtttaat ttgtgcttta acatcttcat taccattttg catagtttct aattgattaa
     2641 ccgaacgata ttcatcatta tctaaaatgg tttctgtaga cacatcttca aagtatataa
     2701 ttgaaatatt tacattcgga cgactactat cttctatttg tgctatatct ttttcaaatt
     2761 ttaaatcatc tggaattgac tcagattgat gtttagataa aatactataa ataagatgga
     2821 acggatttgg tgaagttaat cgttcattga cagcaatgtg ctcaccagaa atagacaatt
     2881 gctctagttc tagtaacgat ttatcatttt tcactctacc aattaaataa agttgttctt
     2941 tcgctcttgt taatgctaca tagactaatc gcatttcttc tgatacaagt tctttttcgg
     3001 caacagctct aaatgcaacc gaagctaaag atggaaatgc catttcttta tccacatcaa
     3061 aataatccat tccgagacca aattgctgat ttaaaataac tggttgtttc aaatcacgtt
     3121 tattaaaatc ttttgacaat ccagaataaa taacaaatgg aaactctaga cctttactac
     3181 tatgaattgt catcattcta acgacattat cgtttggacc aactacgttt tcctcaccaa
     3241 aatctttgcc tctttcaatc aattcatcga taaaacgaat aaattgatat aaacctctaa
     3301 aacttgaatt ctcaaactcg atagctttat taaataaacc ataaagattt gcacgtcgac
     3361 cacgtccacc aataagtcca ctaaagtatt gaataacata atgatcatta taaaatttat
     3421 caattaactg atacaccgga tgatctttac tatattgttg gtaactttga atatctgata
     3481 aaaacatttt taatttatca actaaaatag cgtctgctgc ttcgtcatta atgtaattta
     3541 caatcgattg atagaagtag tcatcatttg gactcaatat tctaatttga gctaattcgt
     3601 cttctttgaa ctgataaata acggaacgca ttaacccaac taaataaata tcttgtaatg
     3661 gattatctat cgctcttaaa aatgataata ctaaacggac ttctgtttgt tcaaagtatc
     3721 cttcacgact attcacatgg aatggaatat cttcattttt aaaggcttgt tgtaaattgc
     3781 gagcttgtcc aaagctgcgt tctagaataa cgatatcctt gtatgtcgca cttctatagc
     3841 ttcctgtttt catatcataa actttttgat gttctaagat atctttaact tgttctacta
     3901 taaaatgcgc ttcttgttca ctaccagtta aatcactatg ttcttgatcc gcttcaacaa
     3961 gcacttttaa gtttactgga tggtccgatt catcatatgg tgcaccataa tacaattgtg
     4021 ctgattcatc ataatttact tcaccgactt gttcatccat catatgttta aaaatatagt
     4081 ttgtcgttga cagtacttct ttacgtgaac ggaagttttg cgacaaatca attcgacgtc
     4141 cagtgccatc tccatctaaa gtaaaccgtt gatacttttc aataaataaa cttggatcag
     4201 cttgtctaaa tttataaatg gactgcttaa catctccaac cataaataaa ttaccattat
     4261 gttcatcacc cgttttgatg caagatagta ttttctcttg aactcggttc gtatcttgat
     4321 actcatcgac caatatttct tggaagtgtt gacggtatga ttcggcaatt tctgaaggcg
     4381 aaccatcctc attagttaaa atttgtaatg caaaatgttc ataatcagaa aaatccaaaa
     4441 tatttttgct acgctttttt cgattgaatt ctgacataac atctttcaca atacgcgcaa
     4501 ggtactttac tcgtggcgcc aattgttgca tatcagcttt caaatcatca gcttctcttg
     4561 aaaagtaatc actcttcact ttatcaatta atgatttata ttttttatac tgaagtttag
     4621 catcttctaa ggcatcgacc atcatttcat ttgcttcttt aattttcgct gttacattag
     4681 gtaaacgcgc gccaaattcg tgaccagtta aataaggtat atcaataaag ccaccttcta
     4741 aaacacgccc cattaaacgt cgttcatctt ctataacagc taaatgtttg tcgacgccat
     4801 ccatcatact aaacaaatca tacgccttat ttaaagcatc ataggcagct gtgataaata
     4861 ctttagataa gtctgttagt agttgaataa gttgcgcttg ttgtacttct tcttcgtatg
     4921 gtgtcaccaa ttgatccaac caatttgtag gatttggatt tgcaacgcta aagaaataca
     4981 attgtttaat aatcattcga aactgatcat cacttctatc tgaagacaat tgctctgtta
     5041 attcaataaa agcaggatca aggatatcgt aatgttgttc tatgacttca tctatcgttt
     5101 gttctaataa taaaatattt tcagcttcac tgcttgttct aaagttcggg tcaatattta
     5161 atacatcata atgctgttga attaatttca agcaaaaact atgaagtgta gatatttgtg
     5221 cttgatgaat tttgatgcgt tggtttttca agtgtgcatt tgcaggatca gcaatcgatg
     5281 cctcttgaat acgttggtct acacgatgct tcatttcacg tgcacttaag tttgtaaacg
     5341 ttacaactaa aagtcgatcg acatcaatgc catcacgtaa aatcttttgg ataatacgct
     5401 caactagtac agctgtttta cctgaacctg ccgcggctgc aacaagtaca tcttgtccag
     5461 ttgcgtaaat actttgccat tgcgcgtcag tccaaatcac gccttgtggt ttctctggaa
     5521 ttgtcatcta ttgctcaccc ccaaattcat catcaatgtt aatattttga attgcttcaa
     5581 ttggatttat tgtttcatct acagttcgat atcgcttact atcaatcatg ccatctacat
     5641 gacataccga ttgataacta caaaaagcac atggcaattt ttgtttgtac tttaatggtg
     5701 caacttcagt atgtccatcc ataatatttg aagctgtttc tataaaattt tctttgttat
     5761 gttggatgaa tttataaatc gttgcttcat ctgcaacttg gctgcctcgt ttactcaaag
     5821 agccatcttt attcaaacca actggtacaa tatctgaagt gaatttaggt tctaaacgaa
     5881 tatccaatgc atcaataaca gtttggtctg aattcactaa accactcagc ttaaactttt
     5941 taattaaatc ttgttctagt ttatcttcat caatatcagc ccatgattta aatttaattc
     6001 taggttcatg tacatggaaa tataataatc cgcctggttt cacaatatct gttaatccaa
     6061 ggcgttgttt attttgtaaa acgatatcca tgtatgtcat catttgcatt tgcataccat
     6121 aatatacttt tgttaaatca agtgtcgcac taccttcaga ggatttatag tcaatgatat
     6181 taacaaaact tgtatcattc tttgtatacg tatcgatacg gtcaatttgc cctctaatat
     6241 taattggaat accttgagtt gtcgttaatg tttgtgcaat taattcatca tttgttcttg
     6301 gtttccttct aaaacttgtc tcaaaatgtt ttggcataaa ctttgaatac gtgccttgat
     6361 attttaatgc gcttagtgtt gtttctacaa tagcgccaat gcgtcttgat aaataacgat
     6421 agtaagctga agaatttaat aaattaaact gaactttagg taaaatttct tccaatgctt
     6481 cattcgttaa ttgtcttatt tttttcaggt ctaatggttt aaaatcgcca ttaatacgtt
     6541 cagatatata ttttaaaacg gaatggaaaa tatcacctaa atcaaagttt tgaagttcat
     6601 acttcgttcg ctcatttagt ttcagaccat gtgaggcata gtgtttaaat gggcattgtt
     6661 gataaccttc aaatcgagat acactggcat tgatttcctt accatataaa tctttagaca
     6721 acgtttcacc taattttaca gtttcattgt caaacgttaa tgctgacatt aaataatcta
     6781 aaccttgatt taaatgatcg ctatctctaa ttacttgata agcatctaac caactatcag
     6841 ccacaatttc atcatctaac caagcacgca atgcttcaaa taatgtaatt ttggtttgct
     6901 tagcatgttg cattagtgac aatgggttaa cttcatggta ttgaggaatg ttagtaattt
     6961 ccaattggtt gaacaatgat tgaatttgat ttaaaaatgg gctgatttcc ttatcatcac
     7021 cacttgatcc cattagacta taagaaaatg taacatttcc cttagctcta gtcatagcaa
     7081 cataacaaac aaatgcttca tccatctgta aaatatctga agtaggactc aactctacat
     7141 ttgcttgttg ttcaaaatat ttcttttctt catcagtaat taaacttgat gcagttactg
     7201 gttgtggcat ggtgccgtcg ttcattccaa ctaagtaaac atgttgctta ttgtcgactt
     7261 tagccaaatc catcgtacca atactaactt gatctaatgt ttgtggaatc atgacaaatt
     7321 ctaattgttc taaaccaata tcaaatactt ctaagaaacg ttccatcgac attggttcat
     7381 ctccaaatac tagaactaag tcatcaagga tttgaattaa gccattccat atttgatcaa
     7441 tttcctccgc cttttcatga ttaccattta aatcaagttc atctcgctct gtcatcaatt
     7501 gatttggcag ttcgaaatat tccatacttt cataaaaagc agttgcaaag tcttttacag
     7561 tttccgcttg tgacatttgc ttttcaaaat gtaaaatttt atcaaagaca tctttcttta
     7621 acttaacgac ttgttcaaat gtgttacgtt catcttcggt cagtttatgc gctttacgcc
     7681 ccattttgct aaaatgttca acattaaata gctcatcatc taaccaacgt ttaccgtata
     7741 taccacgttc aagtacaaaa ttttcaagta aatcaactaa gtatgcactt tttagatatg
     7801 atgccgttaa cacatcagtc ttcaataagc gtagcattgg attcacttgc caattagatt
     7861 gaataacttc aatcaatgaa cgaatcattt ccatgaccgg atgatgtgtc atcgaacgct
     7921 ttgtatcaat gttataagga atattataaa gcggtaatat ggaatcaaat aaataagcat
     7981 aagactcgtc acgatataaa atagcaatat cttgatatcg taattgctta tcacgaatgt
     8041 caacgatgat acgtcgtgca atttcattta tttcctctct catagtccca gattctaaaa
     8101 tattgatatg accttgacat gccactcgat tgatttgaag tgcatcaaat tcttgttcaa
     8161 gatgctttaa atcttgatta ttaaagcgat atgattgctt gaaatattga cgttcaatag
     8221 aaatattgag ttcatttgct atttcttcaa tatgtcgtaa cacttccgat ggtttcctaa
     8281 ataaactgaa ttgatcgtgg ttaccatctg tcgtcaatat aattgtgaca ctcttcgcat
     8341 atttaattaa tcctttgatt attaaatact caatcgttga aaagttgtga aaaccatcaa
     8401 tatatatatc agcacatttt agccactctg atttcggcat acaatcaata aaatattgta
     8461 atgcatcctc acccgtaata aactcctttt gaatgcgttg ttcgaactct cggtatatta
     8521 aagcaatatc ctctaactta tttttagttc gagtttgcat atttttatca gcaataaagc
     8581 tttctaaatg ttcaggcgtt actgcatatt ttttaaaatc ttgaatttgt tctgttaatt
     8641 tttcactaaa cccataatat tttgcttgtg attgataaag ttttaaatac ttttgttgtt
     8701 cttgaacaat gttataaatc atcatttccg ttgcagcttt agataacttt tgttcgctat
     8761 aactaccaac ttcttggaaa atacgatgac ttaatcgttc aaaatgcaac acttctgttc
     8821 ttaaactacc atttaattcc ggatcattga caaaggcttg ttctaattga aatgtacttt
     8881 gagttggcgc aattaaaatg attggatctc caagcggatc tgctttcatt ttttgtttta
     8941 tttcggtcaa cattttcgta gacttacctg ttcccgctct acctaaataa gcatgtaatg
     9001 tcatgacaaa ccctcacttc ctttgacaca tattttaaca caaagtgatt gtgattgatt
     9061 tattataagt tcatatcgaa cttagctttt attgtggtac taaaaaaata aaacatcgtt
     9121 tatcagtatt aaaaccgaca aacgatgttg tatttgtttc atattaattt ttagtatttt
     9181 caggattgaa attatattta aattcgctga atggccagaa tctaaatgaa actttaccaa
     9241 caatttggtc ttcatcaatg aggccaaacg cacggctatc tttacttact tcacgattat
     9301 ccccaagaac taaatattta ccttttggaa tgacatttga tttagggttc gcattcggta
     9361 aatctttaac ttggaaagtc ccagtaatgt aatcaccttg tttatgtttt aaattgtagt
     9421 ttaaatatgg ttcatcttgt tttttgccat tgacatataa tgtatcattt ttatattcta
     9481 ctttatcgcc aggaacaccg atgacacgtt taacatagtc atcatttttg tttgcatgga
     9541 agacaactac attacctttt tccaaaccac ctgttttata tccaataatg tttacagcta
     9601 ctcgctcgcc atctttcaaa gttggatcca ttgattcacc tttaattgta tatggtgtaa
     9661 caataaattt acctactata aacaaaatga caaaagcgac tgcaattgaa ataatccatt
     9721 ccaataattc ttttttcaat tttgacacct ctttttaaga tttgaactga atcgtccatt
     9781 ttgaaaaagg ataatatctc aaacttatat taccaataat atccttttta tcaattaaac
     9841 caaattgtct agaatcatgc tgattgttat catgatcatt tagcacaaca aaattgttag
     9901 gcggtataat atctccatct aattctttaa aattgcgcaa actaaaatct ttaatttttc
     9961 tgttcttagc ataagatgcg tcaaccggtc ggtcatcacg gtataattgt ccctgacgaa
    10021 acgccattga ttgaccaggt ttggcaataa ttcgactagt atatatctcg ttaccacgcc
    10081 tatatgtaat gatatcacca ttattcaatt gattaaatgt aactttaatt ttatttacaa
    10141 taacacgatc ccctttgttc agggttggtg acatatcatt attcggaatg acatgaccaa
    10201 ctattacaaa agtttgtacg aacagtacaa tgataatagc aagtatcaat gaaatcaaat
    10261 attttacaac ttttttcacg atgtcactcc tttttcgacc ccataaaatg ctgttctaac
    10321 ttttttccaa caatccaaaa tacaatcaac acaactaaca ttaatatccc tcttaaagga
    10381 tgcgttaaaa ttgtagtaat ttcctttcct aaataaccta aaataattgt tgaaactaac
    10441 tttgatgatg ccaaaacaat aaaataatat ttaggtctaa tatgagatag actcgctaca
    10501 aaatttatta atgtatttgg cgtaaaagga aaacaaagta aaataaacaa tgggattaat
    10561 ccttggcgat caataaaact aatcaagcgt tgaacagcag tacgttgttt aattcgctgc
    10621 atcctttcag tgttcaccaa tcgtttacag atcaaataga ctgtaaatgt tccagaaatt
    10681 aatccaagcc aactaatcaa tatacctaaa ataggtccat aggcttgaat attaattaaa
    10741 atatagagtg ctaatggaaa tactggaata atcgctctta tatataacaa tatgaatcca
    10801 ggtaaataac caaactgtcg aaatatctca aaccattctt ctacttgatg aaacgacaaa
    10861 tcatcaatcc ctttctttgg ctgaagataa ttattcttac attataaagt taattactaa
    10921 gtaaagaaac attaacatgt aatcatgcta ataaatcagt ctctagtagt ataacaattt
    10981 tattgtatgg cagatattat tttctcttaa catcttgtca cattagctag taacctagtt
    11041 cttgtatata aataaaaacg aggttgtcat tacattttta catttgtaat gacaacctcg
    11101 gaaatatgct ttaaatagtt aacatctagt tctatctttt caaacttcac taatctttga
    11161 agtaatgtat tttataaacg ctcttctaat tcttttttca agtcttcaaa accaggttta
    11221 cctaataatg cgaacatgtt ttgtttatat gcttctacac caggttggtt aaatggattt
    11281 acgcctaatt ggtatccact cattgcacaa gcaagttcga agaagtatac gacataaccg
    11341 aaagtttctt catctaattg tggaatgttc actaccatgt taggaacacc accatcagta
    11401 tgcgctaata atgtaccttc gaatgctttt gtgttaactt cgtcgattgt tttaccagct
    11461 aaataattta atccgtctag atcatcacta tctttttcaa tagtaatatc atatttagga
    11521 tgatttactt ttacaactgt ttcgaataag aaacgacggc cttcttgtac atattgacct
    11581 aaagaatgta aatcagttgt gtagttggca cttgaaggat agataccttt gaagtcttta
    11641 ccttctgatt caccaaataa ttgtttccac cattcattaa agtattgcat agatggttca
    11701 tagttaatca acatttctgt tgtataacct tttgcatata aaatgtttcg aattgtcgca
    11761 tattggtatg caatgttttc ttctaattta tctgaagata attcttcacg tgcttttgca
    11821 gcaccaatca tcatagcttc gatgttaatt ccagctgttg caattggtaa taatcctact
    11881 gctgttaaaa cagaatatct tccacctaca tcatcaggta cgataaatgt ttcataacct
    11941 tcgtttgtag ccaactgttt taaagcacct ttttctttat ccgttgttgc aaatatacgt
    12001 ttttgtgctt cttctttacc gtatctttct tcaactaatt gtttgaacaa tctaaatgca
    12061 actgctggtt ctgtagttgt accagattta gaaataacgt ttacagagaa atctttgtct
    12121 gctaaataat caactaactc tttagtgtat gttgatgata agtgattacc aacaaataca
    12181 atttcagggt attcattgct gtttctaaat gatgacgtta acatttcgat tgctgcacgt
    12241 gcacctaaat aagaaccacc aataccgatg actactaaaa catcagaatt ttctttaatg
    12301 cgttttgatg cttcaacaat tcttgaaaat tcttctttgt cgtaatcaac tggtaaatca
    12361 acccagccta agaagtcact acctgcacca gtaccttcat gaattgtttt gtgaattgat
    12421 ttaacaattt cttgttgttg ttttaattcg tgttcaccga aaaattctaa cgttttacta
    12481 aaatctaatt gaatatgagt catctataaa agcctcctgt ttcatttaat atcttaattt
    12541 tactaagttt acgtttgcga ttcaattcac aagtttgtac ttaatcaata aaattgatat
    12601 ttatgtgacg tttcacacct atttttcaac aaacatttca aactgtcgtc aaacactcaa
    12661 acattcttta tacaatttgc atgtttttta aaacactttt aaacattgat aaataagcat
    12721 ttatactaac aactctaact acctctatca tattttatga ataaatatgt aaaataatgg
    12781 ttttattttc attaaaagtt tgctatcatg tgttcatacg aaatattcag ataactacaa
    12841 tgaggtgaat tttatgaaag agaaaattgt tttagcatat tcaggaggac tagatacaag
    12901 tgtggccgtt caatggctta tcgacaaagg atacgacgtt gtagcttgtt gcctagatgt
    12961 tggtgaaggt aaagatttag acatcgttta taaaaaagct ttagatatgg gagctgttga
    13021 atgtcatatt attgatgcaa caaaagaatt tagtgatgag tatgtaagtt atgcaatcaa
    13081 aggaaattta atgtatgaaa atgcatatcc attagtttct gctttatcaa gaccattaat
    13141 cgctaaaaaa ttagttgaga ttgctgagaa aacaaattca gtaggtattg cacatggttg
    13201 tactggtaaa gggaatgacc aagtacgttt cgaagttgcc attaaagcat taaacccatc
    13261 attgaaagca ttcgcacctg tacgtgagtg ggcatggagt cgtgaagaag aaattgatta
    13321 tgcaattaaa cataatatcc ctgtatcaat caaccatgat tcaccttatt ctatcgatca
    13381 aaatctatgg ggcagagcga atgaatgtgg tattttagaa gatccttatg ctgcgccacc
    13441 agaggatgcg ttcgatctaa caaatgcttt agaagaaaca ccagatactg ctgatgaaat
    13501 cattttaacg tttgataaag gtatcccagt tcaaattgat ggcaaaacat atgaattaga
    13561 cgatttaatt ttaacgttga atgcattagc tggtaagcat ggtatcggaa gaattgacca
    13621 tgtagaaaat agacttgtag gtatcaaatc aagagaaatt tatgaagcac ctgctgcaga
    13681 agttatttta aaagcgcata aagcattaga aacgattacg ttaacgaaag atgtcgcaca
    13741 ctttaaacca atcattgaga agcaatttgc tgaacaacta tacaatggac tttggttctc
    13801 acctttaact gatagcttga aattatttat tgatagtact cagcaatacg taagtggtga
    13861 tgtcagaatt aaattattca aaggtaatgc catcgttaat ggtagaaaat caccttacac
    13921 attatatgat gaaaaattag caacttatac aaaagaagat gcatttaatc aagacgctgc
    13981 tgttggcttt atcgatatct atggtttacc tactcaagta aatgcgatgt tacatggagg
    14041 ctatagcaat gagcaataaa gcttggggcg gtagatttga agtacaacct gaagagtggg
    14101 ttgacgactt taacgcatcc attacttttg atcaaacgct catagatcaa gatatcgaag
    14161 gcagcattgc gcatgcaact atgcttgcga atcaaggcat tattagtcaa caagacagcg
    14221 aacaaattat acaaggacta aaatctattc aacatgatta tcatcaagat caaattcaat
    14281 ttagtgcatc attagaagat attcatttaa atattgaaca tgaattaatt aaacgtattg
    14341 gtgatgctgg tggtaagttg catactggac gcagtagaaa cgatcaagtt gcaacagaca
    14401 tgcacttgta cactaagaaa caagtgcaag atatcatcgc attaattaag tcgttacaaa
    14461 gtgtaattgt agacatcgct tccaataatg ttgatacaat tatgcctggt tatactcatt
    14521 tacagcgtgc acagccaatt tcatttgcac atcatattat gacttatttt tggatgttac
    14581 aacgagacca acaacgattt gaagatagtt taaaacgaat cgatattaat cctttaggtg
    14641 cagcagcctt aagtggtacc acatacccta tcgatagaca cgagacaaca gcattgttga
    14701 actttggcag tctctatgag aatagcctag atgctgttag tgacagagac tatattattg
    14761 aaacattgca taatatttct ttaacgatgg ttcacttatc acgctttgca gaggaaatta
    14821 ttttctggtc cacagacgaa gctaaattca ttacattatc agatgcattt tctactggct
    14881 catctattat gccacaaaag aaaaatcctg atatggcaga attaattaga ggtaaagttg
    14941 gtcgaacgac tggtcattta atgagcatgc ttatgacttt aaaaggatta cctctagcat
    15001 ataacaaaga tatgcaggaa gataaagaag gtttattcga tgctgtccat acaattaaag
    15061 ggtctttacg tattttcgaa ggtatgattc aaacgatgac aattaataaa gaacgactca
    15121 atcaaactgt taaagaagat ttttcaaatg caacggaact agcagattat ttagtaacta
    15181 aaaatattcc atttagaact gcacatgaaa ttgtaggaaa aatcgtctta gaatgtatac
    15241 aacaaggtca ttatttatta gatgtacctt tagcaacata tcaacaacat cattctagta
    15301 ttgatgccga tatttacgat tatttgcagc cggaaaattg cttaaaacga cgtcaaagtt
    15361 acggttcaac gggtcaatca tcggtcaaac aacaacttga tgttgctaaa caattactat
    15421 cacaataaat acgttaatct acctacccac aatgtctatg aaaattacat tgtgggtatt
    15481 ttaatgctct cttcgtcttg ttgaacatca catttttaag attcctaaaa tgtttgataa
    15541 ttcttttaaa tttatattac aaaaatgtta taaattgtaa aagaaatgtg taaagcgttt
    15601 tcacaagcag gtttttgtag tattttaaaa ttgttagact acaaataaag agatgaaagg
    15661 ataaagacta tgactaactc ttcgaaaagc ttcactaaat ttatggctgc ttctgctgtt
    15721 tttactatgg gatttttatc agtacctact gctggcgctg aacaaacaaa tcaaattgca
    15781 aataaacctc aggctattca atggcataca aatttaacga atgagcgatt cactactatc
    15841 gcacatcgtg gcgcaagtgg ctatgcaccc gagcatacgt ttcaagcata tgataagagt
    15901 cataatgagt taaaagcatc gtatatcgaa attgatttac aacgtaccaa agatggccat
    15961 ttagttgcta tgcatgatga aacagttaac cgtacaacaa atggacacgg taaagttgag
    16021 gattataccc ttgatgaatt aaaacagtta gatgcaggaa gttggtttaa taaaaaatat
    16081 ccaaaatacg caagagcaag ttataaaaat gctaaagtac ccactttaga tgaaatttta
    16141 gaacgttatg gtccgaatgc aaactattat attgaaacaa agtcacctga tgtatacaca
    16201 ggaatggaag aacaattatt agcttcattg aaaaagcatc accttttaaa taacaataaa
    16261 ttaaaaaatg gacatgtaat gattcaatca ttttctgacg aaagtttaaa gaaaattcat
    16321 cgtcaaaata agcatgtgcc attagtaaaa ttagttgata aaggtgaact acaacaattt
    16381 aacgaccaac gcttaaaaga gatacgctct tatgcgattg gattaggtcc tgattataca
    16441 gatttaactg aacaaaatac ccatcattta aaagacttag gatttatagt acatccttat
    16501 acagtaaatg aaaaagctga tatgttacga ttaaataaat acggcgttga tggtgtcttt
    16561 acaaatttcg ctgataaata taaagaagtc attaagtagg attattaata ttcaaatagt
    16621 tgtttaaatg ataattctag ataatatttt agttattaca acataaaaaa gcagtaagtt
    16681 tattttcccg tagaaaatat cttactgctt ttttctaatt cataattcaa tatatttaat
    16741 tatgcccagc cacgatatct agctgcttct gctgtacgtt taatacctat gatatatgct
    16801 gcaagtctca tatcaatttt tctattttga gacaattcgt aaatcgtatc aaatgccgct
    16861 tctaattttt cacgtagctt ttcatttact tcttcttcag accaataata accttgatta
    16921 ttttgtaccc attcgaagta agaaaccgtt acaccaccag cacttgctaa aacgtctgga
    16981 actaataata taccacgttc agttaaaata cgtgttgctt ctggtgttgt aggtccatta
    17041 gcagcttcaa caacgatgct agctttaata tcatgtgcat tgtcttctgt aatttggttt
    17101 gaaatagccg ctggtactaa aatgtcacaa tctaattcaa acaattcttt atttgagatt
    17161 gtttcttcaa ataaatttgt taccgtacca aaactatcac gacggtctaa taaataatct
    17221 atatctaagc catttggatc gtgtaatgca ccgtaagcat cagagatacc tacaattttt
    17281 gcacctaaat catataagaa tttagctaag aaacttccgg cattaccgaa accttgaata
    17341 acaaccttgg caccttcaat ttgcatatta cgacgttttg cagcttgttc aattgcaata
    17401 actacaccta gtgcagttga tctgtcgcgt ccatgagaac cacccaatac aattggttta
    17461 cctgtgatga aacctggtga attaaattta tctaatgcac tatattcatc catcatccaa
    17521 gccataattt gtgagttagt aaatacatct ggtgctggaa tatctttgtt cggacctacg
    17581 aattgtgaaa ttgctcttac atatccgcgt gataaacgtt caacttcatg aatgctcatt
    17641 tgacgtggat cacaaacgat accaccctta ccaccaccat atggtaagtt aacaatgcca
    17701 catttcaaag tcatccacat tgataatgct tttacttctt cttcatcaac atctgggtgg
    17761 aaacgcacgc ccccttttgt tggtccaaca gcatcattat gttgcgcacg gtaacctgtg
    17821 aatgttttaa cagtgccatc atccattcgt acagggatac gcacttgtaa cattcttaaa
    17881 ggttctttaa ttaaatcgta cattccttcg tcaaatccca atttatgcaa tgcttcttta
    17941 ataattcctt gagtagaagt tactaaatta ttgttctcag tcatgatcct tttcgcctct
    18001 tctttaccta atgatttcgc tttcaaacat attgtaacat aacgtattcc tttttaaagc
    18061 ccttacaaac tgattgttac aactttttga cattattgaa atacatgtct tattttttca
    18121 agtgcaaggt ctaattcttc tttagtaata attaatggtg gtgcaaaacg aatgacagta
    18181 tcatgcgttt ctttacataa taagccttct tctttcaaag cttcacaata tggtctagca
    18241 ctttcattaa gttccacacc tataaacaaa ccacgcccac ggacttcttt aattgatgga
    18301 tgattaattt gctttaattg ttctttaaaa taatctccta attctaaaga gcgccctggt
    18361 aaatcctcat caacgataac atctaatgca gcaattgatg cagcacaagc aagtggatta
    18421 ccaccaaatg ttgaaccatg tgagccaggt gtaaagacat ctaatacttc tttatctgct
    18481 aatacaacag aaattgggaa gacaccacca cctagtgctt tacctaaaat atagacatca
    18541 ggttttacat tatcccaatc cgtagcaaat aatttacccg aacgacctaa tcctgcttgg
    18601 atttcgtcag caataaataa tacattatgt tcatcacata attctctaat tgctttcaaa
    18661 tatccttctg gcggtatatt tatacccgct tcaccttgaa ttggttctac taaaactgct
    18721 gcagtatttt cattaattgc agctttcaat gcatctacat ctccaaaatc aacttttcta
    18781 aatccatcta ataacggacc ataaccacgt tggtattctg cttctgatga taatgaaact
    18841 ggcgccattg ttcgaccatg gaagttacca ttaaatgcaa tgatttctgc tttatttggc
    18901 tcaattcctt taacatcgta tgcccagcgt cgtgctgctt tcaaagctgt ttctactgct
    18961 tcagcacctg tattcattgg taaagcttta tctttacctg ccagtttaca aattttttcg
    19021 taccattctc ctaagttatc actatgaaaa gcacgtgaaa ctagagtcac tttatcagct
    19081 tgatctttta atgcttgaat aattttcgga tgtctatgac cttggttaac agcggaatat
    19141 gcagataaca tatccatata tttattgcct tcaggatctt taacccatac cccttcagct
    19201 tctgaaatga caattggcaa tggtaaataa ttatgtgctc cgtaatgatt tgttaactca
    19261 ataatttttt cagatttagt catcatatct ccccttttca tcatttataa ctattataca
    19321 tgaaacatta tccaaataat tacattagtt ttcaaagcag atacttttcc accaaaaaag
    19381 atgaaataat cactaagttt cattaaattt gtctattttg aaaaccctta catttataat
    19441 gacataatta cttaaatgat tacaagcaaa agaattgata attttacact taatcaaaag
    19501 tatattttac taagaatatt tttatttata aatattgaaa accactaaca aattgcatac
    19561 acaatatcat tagtggtaac agttaaacac ttatttatct ttacgtggta atggatagaa
    19621 cccttcatta aattgatgcc ataaattagg agttaagtga tgattatctt ctctctctgg
    19681 atcaaagtaa ttaataattt catcttcccg tccatcttta attttttcaa caaattgata
    19741 atccagtagt agctctctac caatggctac taagtcaaca ccaacatttt caactgcatc
    19801 taaagcttcg tcagctgtga aaattgaacc aataccgata agtggcatac gaccatttat
    19861 ccatttgtga attaaaggta atctttcttg tccagcgtat ttaccttcac gtgtcgttgc
    19921 atgcgtatcc atcattgaaa catgaatata gtcgattggc atatggctaa ttttattaac
    19981 gagttcctct gtaatttcca ttgtgattcc tggagactcc gcttcctctg gagataatct
    20041 gtatccaact ataaagtctt tattgccata tgcttctttc gctttaagca cttcttcaat
    20101 cacagcgacc gggaatttat attgatttgc ccatacatca tttcttctat tatagtatgg
    20161 tgatacaaat tgatgaatta agtaatgatt cgcgccatgt atttcaacac catcaaaccc
    20221 tgcttcaatt gcacgtcgcg ttgcttcacc aaaatccttg attgcttgtt caatctcttc
    20281 attcgtcatt tctctagcac tatgttcttg tttctgaccg aaacttttta aagaaattgg
    20341 acttggtgct acgacgtctc catcaggtgt taattcaggc aatgcttgtg caccgccatg
    20401 atgtatttgt acgagtgctt tggcaccgtt tttcttcatt gctgtagcta atcgttttag
    20461 tccttcaata tcactgtcat gcgcgattga tggctgtcct ggaaatgctt ttccgacatc
    20521 actcacatta ctcgcagcat taattgtaat accaacatct tgtgaacgct tttcaatata
    20581 aggaagttct acatctgaaa tagtaccatt atcatttgaa gaaatatgtg ttaaaggggc
    20641 taacacaaat cgatttctca actctactcc atttggtaat tctactttat caaacaatgg
    20701 ttcatattta cttttcatta cttaattcct ccttaaaatt attgagttga ctataacatg
    20761 taattttttc attctccaat tatctgttta tatagttgca attcgtttaa tactttaagt
    20821 tgtagttctc atttctaaaa taaattaaaa aaacaaatcc acatacatta ttgcaaatca
    20881 acgattctat ctaacaaaga taacggataa gtcaccttaa accatttgta cgtgtaaaaa
    20941 gaatcaatca tattacgtta tcgtagacaa aaagagatta gtatctaata aaatttaata
    21001 gatactaatc tcttttatag tctaagaaac tttcatttca gaccctttgt atgattttcg
    21061 gtacgattta tctgttcctt tagtcgtttc gaattgctcg ctttgactgt tttatccaga
    21121 ttggtaagcg ttctttaagt gtttgaaacc catatttttc tgtttctctg atttcatcta
    21181 atactgattg tttttccttc ttacgctcat aatttttgaa gtaatcatta tcctttaatg
    21241 atagattaag ctttccttca tcatctatag acaaaatttt agctttaaca atttggcctt
    21301 ctgatagaaa tttcttcaaa ttatgaacgt agtcatccat aatttctgat atatgaatca
    21361 gtccttcagt atgattaggg gtctcaacaa acgcaccgta tggttgaata ccagttacac
    21421 gcaccttgat atgttggcca attttgtagt tattcaactt aataacccct tatttcgttt
    21481 acttaacact attatcatag cacaaatcga aatggtggac taattaaaac atcaaatcat
    21541 acgaatatta ttttataaat gaataagaat tctacaaact tggatttaca aataaaatca
    21601 gcaacaaatt cctatctaaa tcaatatgat atccagctac tttgttttca atttactaac
    21661 tatttgaata ccaattctct gtaaaaatat aaaaagcaac ctcaactatt atctttagaa
    21721 tacacatcta tttaaaaact gtgctttatt gataaatata ttgagattgc tcatcatgtt
    21781 a
//
LOCUS       DAHHRR010000036        20700 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.036, whole genome shotgun sequence.
ACCESSION   DAHHRR010000036 DAHHRR010000000
VERSION     DAHHRR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20700
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.036"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..942
                     /locus_tag="NBI42_001183"
     CDS             <1..942
                     /locus_tag="NBI42_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCD1777610.1"
                     /translation="NIYFEEKDSKHYICEDDVIQWIPLKSLYHPDRDVLIPLQMLVLF
                     DKTIFKDEKQFTNSGVSTGTASHETFEKSLTNSIIEILQIDSYNLWWYGGIEGKAISV
                     NIAEKLNEWFKRESIVHKFLDNFDVSFTDISFDKPIHVVVCEVKSKAKDLSLPKYVVG
                     VQGDTNLEQCIYRSLLECVTVLEYTMTEVWSNTTHFKNISENIKNHPIDNLDDNVMYY
                     AKYGLNFNMHKRKITFQNIKNAKNLHEIINELKHLSYYACYLDITPVEFYNKNLVVVR
                     SFIPELLTIALPSYPPYLHPRYKEIGGIINDIPHPLA"
     gene            1653..2024
                     /locus_tag="NBI42_001184"
     CDS             1653..2024
                     /locus_tag="NBI42_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1777611.1"
                     /translation="MEKLNQVMLYVDDVDKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKYSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            1963..2199
                     /locus_tag="NBI42_001185"
     CDS             1963..2199
                     /locus_tag="NBI42_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777612.1"
                     /translation="MVNVYLISKMAKVTILQLVTKQSFLIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLLLYRVWKRFPIQ"
     gene            2218..3237
                     /locus_tag="NBI42_001186"
     CDS             2218..3237
                     /locus_tag="NBI42_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1777613.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNLNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGDFEVSKDRIQGFETIASQNKINYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTSIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            3253..4902
                     /locus_tag="NBI42_001187"
     CDS             3253..4902
                     /locus_tag="NBI42_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCD1777614.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNSWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(4985..5851)
                     /locus_tag="NBI42_001188"
     CDS             complement(4985..5851)
                     /locus_tag="NBI42_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1777615.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(6081..7565)
                     /locus_tag="NBI42_001189"
     CDS             complement(6081..7565)
                     /locus_tag="NBI42_001189"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCD1777616.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLEF
                     SNELDSKFKLNGNRLFYLAMAPQFFGVISDYLKSSGLTDTNGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRNEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            7880..8800
                     /gene="rnz"
                     /locus_tag="NBI42_001190"
     CDS             7880..8800
                     /gene="rnz"
                     /locus_tag="NBI42_001190"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCD1777617.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
     gene            complement(8903..9718)
                     /gene="proC"
                     /locus_tag="NBI42_001191"
     CDS             complement(8903..9718)
                     /gene="proC"
                     /locus_tag="NBI42_001191"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCD1777618.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            9860..10615
                     /locus_tag="NBI42_001192"
     CDS             9860..10615
                     /locus_tag="NBI42_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1777619.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLDNKVRVTLLVRDVDKATRIF
                     EQEIGKTINVIPCDLNDMKSIQALKFENNTSFDGFIYSAGLGYFKSISEHSVSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFFTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGKIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            10631..10879
                     /locus_tag="NBI42_001193"
     CDS             10631..10879
                     /locus_tag="NBI42_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777620.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILSDISVDQFSDSTL
                     YYTCEKFKLLVLQGNVLGVQVITNNDESICEVKYRKMF"
     gene            complement(11095..12003)
                     /locus_tag="NBI42_001194"
     CDS             complement(11095..12003)
                     /locus_tag="NBI42_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1777621.1"
                     /translation="MQKNILKSGIALSELGLGCMSLGTDLKKAEQIIDCAIENDITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIVIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDQTISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFNYLTSHDVLGSIIVGASSVDQLKENIDNY
                     HAKVSLDQIKTARARVKDLEYTNHLV"
     gene            12085..12627
                     /locus_tag="NBI42_001195"
     CDS             12085..12627
                     /locus_tag="NBI42_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCD1777622.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLQEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            12732..13181
                     /locus_tag="NBI42_001196"
     CDS             12732..13181
                     /locus_tag="NBI42_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD1777623.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            13230..14117
                     /gene="xerD"
                     /locus_tag="NBI42_001197"
     CDS             13230..14117
                     /gene="xerD"
                     /locus_tag="NBI42_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCD1777624.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELEHVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(14195..14701)
                     /locus_tag="NBI42_001198"
     CDS             complement(14195..14701)
                     /locus_tag="NBI42_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HCD1777625.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFYKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPDFERIIKANYSDYDYNHETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            14793..15524
                     /locus_tag="NBI42_001199"
     CDS             14793..15524
                     /locus_tag="NBI42_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCD1777626.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDFDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIVRGVNYHFG"
     gene            15517..16059
                     /gene="scpB"
                     /locus_tag="NBI42_001200"
     CDS             15517..16059
                     /gene="scpB"
                     /locus_tag="NBI42_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCD1777627.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDISKDQLVELIENYSS
                     HGLIIQRFGTTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            16052..16789
                     /locus_tag="NBI42_001201"
     CDS             16052..16789
                     /locus_tag="NBI42_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCD1777628.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIDARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            16922..17647
                     /gene="srrA"
                     /locus_tag="NBI42_001202"
     CDS             16922..17647
                     /gene="srrA"
                     /locus_tag="NBI42_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HCD1777629.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            17628..19379
                     /gene="srrB"
                     /locus_tag="NBI42_001203"
     CDS             17628..19379
                     /gene="srrB"
                     /locus_tag="NBI42_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HCD1777630.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDDKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCAENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            19623..20552
                     /locus_tag="NBI42_001204"
     CDS             19623..20552
                     /locus_tag="NBI42_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCD1777631.1"
                     /translation="MKKFIGSVLTTTLILGGCSAMENESNKDTNTETKSVPEEMEASK
                     YVGQGFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEKKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIRKNDK
                     EFKEGMERARKEVNYAANTDAVATLFSTKKNFTKDNTVDDVIELSDKLYNLKNKPDKS
                     TITIQIGKPTINTKKAFYDDNRPIEYGVHSKDE"
     gene            20545..>20700
                     /locus_tag="NBI42_001205"
     CDS             20545..>20700
                     /locus_tag="NBI42_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777632.1"
                     /translation="MNKINDRDLKELSSYWVYQNIDIKKEFIVNGKRFKQIDSYNDDK
                     NSNLNGAA"
BASE COUNT         7370 a         2937 c         3682 g         6711 t
ORIGIN      
        1 aatatatatt ttgaggaaaa agactctaaa cattatattt gtgaagatga tgtcattcaa
       61 tggattccac ttaaatctct gtatcaccca gaccgtgatg ttttaatacc tttacaaatg
      121 cttgttctat ttgataaaac gatatttaaa gatgaaaagc agtttacgaa tagtggtgtt
      181 tcaactggta cagctagtca tgaaacattt gaaaaaagtt taaccaattc tattattgag
      241 attttacaaa tagattcata caatttgtgg tggtacggag gcattgaagg aaaggcgatt
      301 tctgtaaata ttgctgaaaa actaaatgaa tggtttaaac gggaaagcat tgtgcacaaa
      361 tttcttgata attttgatgt atccttcact gatattagct ttgataagcc aattcatgtt
      421 gttgtctgtg aggttaaatc taaagctaaa gatttaagct tgcctaaata tgttgttgga
      481 gttcaaggtg atacgaactt agaacagtgt atctatagaa gcttattaga atgtgttacc
      541 gtgttagagt atacaatgac tgaagtttgg agtaatacta cacattttaa gaacatctca
      601 gaaaatatta aaaatcatcc tattgataat ttagatgata atgttatgta ttatgctaaa
      661 tatggtttga attttaatat gcataaaaga aaaatcacct tccaaaatat taaaaacgcc
      721 aaaaatttac atgagatcat caatgagtta aaacatttaa gttattatgc gtgttattta
      781 gatattacgc ccgtggaatt ctataataaa aatttagttg tcgttagatc ttttatacct
      841 gaattattga cgatagctct accatcatat ccaccttatc tgcatccaag atataaagaa
      901 atagggggaa taataaatga tattccacat cctttggcat agcctgattg gtctaatgtt
      961 ttttctaact tgcttcccag gtatattgct tatttgcatt agaaaagtac cgtattttaa
     1021 atacctaact gaatttcaca agtacatttt ggtactaatt atcgtactga taatagatat
     1081 gtgtttattt ttaagttata ctaatatcta cttatgtgta tttgttataa tcattacacc
     1141 agtaataatt tatattacag aaaaatcatt actgtttaac aaaaatttgt tcgactttta
     1201 taacaaaaac agcaaattta ttttaagcgt gttaattgca tatccaatac tagaagaatt
     1261 aaatttccgt tactttttgt ataatttcag cttgatatat cattatcatt atattcagta
     1321 catcatctta tcagtactaa cctttgtgtt ctctcatttt ctatatcaag gaaagtcttc
     1381 tataataaaa atcttttttg ctacaatgca aacactggtt tatttgtgtt ttagagatat
     1441 tttcatcaat ataattgttc atatgatatt taatatatta gtatttagta gccaaatgaa
     1501 gaaaataaat tattctataa taacaaaata gcggaaatta aaaacatgaa catttattat
     1561 aaagctatct aattaaaaag cattttgtga agtaataaaa tatttactgt gctatgatag
     1621 catgtactat agagaaatag aggtactatc ttatggaaaa gttaaatcaa gttatgttat
     1681 atgttgatga tgttgacaaa gcgaaggcat tttggacaga aactttagaa tttgttgttg
     1741 taagtgaaac accgttagct gaggattatg ttgcagttga agtatcacct acgaaagatg
     1801 ctgaaacgtc attgacgatt atggcaaaag aatttattga aaagtatagc ccggaggtga
     1861 acctaggaac accatcttta atgtttaaag agaaaaactt tgatgcatta tactcaaaat
     1921 taaatgattt aggtttaact gggcatgata tagtagaaat gaatggtcaa cgtgtattta
     1981 atttccaaga tggccaaggt aactattttg cagttagtga ctaaacaatc gtttttaata
     2041 tttatacaaa cctttatatt gattttcgga ttgaatttca gtatgaaggt tttattttta
     2101 tcgttttatg caatcgtttg cacaaatctt ttgacttttt tagaaagtgt gactaaactg
     2161 ttattataca gagtttggaa gcgttttcca atacagtgat taacaggagg tgattaaatg
     2221 gttacgatta aagatgttgc actaaaagcc ggtgtttctc cttcgacagt ttcaagagtt
     2281 ataaaaggaa atcaacgtat tagcgaagcg acaatttcaa aagtgaagaa agttatggaa
     2341 gaattgaatt attttcctaa tactgctgct agaactttaa ttactaacca aacatataaa
     2401 attggtttag tgttaaaagg gtctgaggag cctattcgac tgaatccatt ctacatcaat
     2461 gtgttgctag ggatttctga aacatgtaac cagcatggct atggtacaca aacgacagtc
     2521 tcaaataatt tgaatgattt aatggatgaa gtttataaaa tgattaaaca acgaatggtt
     2581 gatgcgttta tactgctcta ttcaaaagaa aatgatccga ttaaacaaat gttaattgat
     2641 gaaagtatgc catttattgt gattggtaag cctacatcgg atatagatca tcaatttaca
     2701 cacatagata atgataatat attagcttct gaaaatttga cacgacatgt tattgaacaa
     2761 ggtgtagatg aattaatatt tattacagaa aaaggcgatt ttgaagtttc gaaagacagg
     2821 attcaaggat ttgaaacgat agcttctcaa aataaaatta attatcaaat tattgagact
     2881 agtaatgaaa gagaagtaat ttttaattac atgcaaaatc tacatacgcg cttgaaggac
     2941 ccaaatatta aacaggcaat catttcgtta gatgctatgt tacatttagc gattttaagt
     3001 gtcctatatg aacttaatat tgaaattccg aaagatgtaa tgacagcaac gttcaatgat
     3061 tcttatttaa ccgaaattgc gtcaccacct caaaccagta ttgacatcaa gcctcgaatg
     3121 ttaggtcaac aggctggtgc agcaatttta aatatattaa aaaataaagc acaggatgtt
     3181 attgaactcg tcattataga tacagaatta aaaataagaa aatcaacaca gcgatagcaa
     3241 aggagtttta aaatgaataa gcaatggtgg aaagaagcag tagcatatca agtatatcca
     3301 agaagtttta atgatagtaa tcacgatggt attggggatt tacctggaat gattgataaa
     3361 ttggactact taaaagattt aggtatcgat gtcatttggc tcagtccaat gtttaaatca
     3421 cctaatgatg acaatggtta tgatattagt gactaccaag agattatgga tgaatttggg
     3481 acaatggaag actttgatcg tttattaaaa ggtgttcatg atagaggcat gaagcttatt
     3541 ttagatttag ttgtgaatca tacatctgat gaacatcctt ggtttataga atccaaatct
     3601 agtaaagaca atcccaaacg tgattggtac atttggcaag atccaaagcc agatggctct
     3661 gaaccaaaca actgggaaag tatctttaat ggatctacat gggaatatga tgctaatact
     3721 gagcaatatt atttccattt attcagtaaa aaacaacctg atttgaattg gggtaatcca
     3781 gaagttagag atgctgtatt tgaaatgatg aactggtggt ttgataaagg cattgatgga
     3841 tttagagtag atgcaattac gcatattaag aagacgtttg aagcgggtga cttacctgta
     3901 cctgagggta aaacatatgc cccagcattt gatgtagata tgaatcagcc aggtatacaa
     3961 acttggttac aagagatgaa agatcgctca ttaagtaagt atgacattat gactgttggt
     4021 gaagcgaatg gtgtaagccc tgatgatgct gatgactggg tcggggaaga aaatggtaaa
     4081 tttaatatga tattccaatt tgaacatttg ggactgtgga atagtggtga ttctcacttt
     4141 gatgtaaatt cgtataaatc tgtattaaat agctggcaaa aacaacttga aaataaaggt
     4201 tggaatgcgt tgtttattga aaatcacgac caaccacgac gtgtatcgac gtggggtgac
     4261 gatgacaagt attggtatga atcagcaaca agtcatgcag cagtttattt cttgcaacaa
     4321 ggtacgccat tcatttatca aggtcaagaa attggtatga cgaattatcc atttgaaagt
     4381 attgaaacat ttaacgatgt tgctgttaaa aatgactatc aaattgtgaa agctcaaggt
     4441 ggagatgtag atgctttact tgcgaaatat aaagatgaga accgagataa ttctcgcaca
     4501 ccaatgcaat gggatgatac gttaaatgga ggatttacaa atggtgaacc gtggttccca
     4561 gtgaatccga attataaaac tatcaatgtt gcacaacaat tagaagatga gcattcagta
     4621 ttacaatttt ataaagattt aattcaatta agaaagtcta atgatgtata cgtatatggt
     4681 caatttgatt tagtagatgc tgaaaattca caagtttttg cgtacacgag aacattaaat
     4741 gaaaagcaag ttcttattgt aggtaatctt actaaccacg aagcagaatt aactgtacca
     4801 tttgatttaa gtcatggaga agtgaagtta tttaattatg atgccaaagt taatttaaaa
     4861 cagttacgtc catatgaagc atgtgttatc gaactaaatt aacatattaa tatgccccct
     4921 aatagaatct aaggccattt gtaaacctat agatatatta gggggcttct ttgtttttaa
     4981 ttatttaatc ttcgtcataa ggactctgtt taacaggaat ccaaagctga attttagtga
     5041 atgggtcatt aaaggaaata tcgagagggt acacttcaac atataaatca tttcgttcat
     5101 atggcaatgt taactgtaaa cttgattcaa tatagtacca agcttcatta gttgcataat
     5161 caatttcacc ttgtaaattg aatttcgcac aatgtttgcc aggtaaaaat cgactttcta
     5221 agtgtgcagg ataacgttca cttggtacac ctacaaatat ttctaaacca ttttcaagag
     5281 gacaactaat aacaaatagt tcaaatggac taacgtcatt atatcgtcgt aactctttaa
     5341 ttttaccatc aatgagcaag tcttctaaaa aatccggaac attaaaagga tgtgacaaat
     5401 acttagtgtc tataaatcgt gcatatccaa ccaatgaaat atcatctgtc tcttctaatc
     5461 tgtatggata gggtgctctc tcagtagttg ataatttgat atataatcgc tcttgaattt
     5521 gtaattcatc ttttttagta gaagcttgaa taggtgatac gccgtgaaaa tcactaaaat
     5581 catttgcaaa atcatttgaa tttgcatagt gatatttttt agcgatatct acaagtcgtg
     5641 tagcaccatt aatcacatca tttgcagcga gtgtcatttt acgcgcgcgt gcataagctt
     5701 ctggagataa gccgacaatc attttaaatg attgatcaag atggtatggc gaaagaccaa
     5761 cgtaatcact taattcttgc aaattgaaag gctctaataa acgatcttca atataaacaa
     5821 ttgcctgttg tatttgcttg ataacgtcca aaactttcac tccaattaat tcaatcattt
     5881 atttttattc tacattattt ctataaatta tacacccatt tgttcaatga ttattaaaat
     5941 agttttgggc attgtaaaat ataatttcat aatatagtct agaaaaaaag cgaatgatag
     6001 aacaattgat ttacttgatt cgtaatcaat ccttgtcatt cgctcattta tttttgttta
     6061 acatgtgcgt tttaattcaa ttattgaata tcgtcccacc aatggttacc atcacgagca
     6121 agtaataaat cactttctaa tggaccatta gtacctgatt catagttagg gaattctgga
     6181 tcaaccatat tccattcatc ttgaattgca tcaacaaatt tccatgttga ttttaattct
     6241 tcccagtgcg taaagttagt ggcatcacct ttaagacaat cgaataatag attttcatat
     6301 gcatctacag tattcatttt atcttgagcg ctcattgaat aagacaattg aacaggttca
     6361 gtttcgatac cttgtgtatt tttcttagca tttagatgta aagatacacc ttcattaggt
     6421 tgaatattga ttactaatag gtttgaatct aacagtttat cagtttcata gtataagttc
     6481 attggtactt ctttaaattc aacgacaact tgaattgttt tagatttcat acgtttacca
     6541 gtacgaatat agaatggtac accagcccat ctaaagttat caattgttaa tttgcctgaa
     6601 acgaaagtag gtgtatttga atcgtcagca acgcgatctt cattacggta tgctttaact
     6661 tgtttaccat cgatatagcc ttcgccatat tgaccacgaa caaagttctt tttaacatct
     6721 tcagattgga aatgacgcag tgatttaaga acttttactt tctcagctcg gatatcttca
     6781 ctatttaaac taattggagc ttccatagct aataatgcaa ccatttgtaa catgtggttt
     6841 tgcaccatat cttttagcgc accacttgat tcataataac caccacggtc ttcaacgcct
     6901 aatatttcag aagatgtgac ttgaatattt gaaatatact tgttgttcca aaggggttca
     6961 aacattgcat ttgcaaaacg taacacttct atgttttgaa ccatgtcttt acctaagtaa
     7021 tggtcaattc gataaatttc ttcttcttta aatgatttac gaatttgatt gtttaatgct
     7081 tctgcagatt ttaaatcact accgaatggc ttttcgataa caagacgttt aaatccgttt
     7141 gtatctgtca ggccagatga ttttaaataa tctgaaataa cgccaaagaa ttgtggtgcc
     7201 attgctaaat agaacaatcg attgccgttt aatttaaatt tactatcaag ttcattactg
     7261 aattctaata attcttggta actttcttcg ttactaacat catgtctatg atagaagaca
     7321 tgttccataa acgcgtcaat tttgtttgta tcttttacgt gcttttgaat tgatgatttt
     7381 acttgattac ggaaatcatc attagtaatg tcacgacgtc caataccgat gatggcaata
     7441 tgttcatcta aattgtcttg ttggtagaga tggaatattg atggaaacaa cttacgatgg
     7501 cttaagtcac cagttgcacc aaagattgtg attaaacaag ggatgtgttt gtttttagta
     7561 ctcaagatta aaacctcaat tcttaattag atatatgatt tattattata agataatcca
     7621 ttaagtaggt catacaatat gctcgactgt attcgctaac ttaaaaatta attatcacta
     7681 tgtgtatcat gcaaaatgtg atacttatta atgtttagta taacgtaatt atcaaaattt
     7741 atatagttat gtctacgttt aagtttgaaa atttaacatg taaggtagaa tttttaacag
     7801 aatttgatta aagaataaat taaaactaaa ttgatacaaa ttttatgata aaatgaattg
     7861 aagaaaagga ggggcatata tggaagttac attttttgga acgagtgcag gtttgccaac
     7921 taaagagaga aatacacaag caatcgcatt aaatttagaa ccatattcca attccatatg
     7981 gcttttcgac gttggtgaag gtacacagca ccagatttta catcatgcaa ttaaattagg
     8041 aaaagtgaca catattttta taacacatat gcatggcgac catatattcg gtttgcccgg
     8101 attactttcc agtcgttctt ttcagggcgg tgaacaaaag ccgcttacat tagttgggcc
     8161 aaaaggaatt aaagcatatg tggaaatgtc tatgaattta tcagagtcac atttaaacta
     8221 tccaattaca tatattgaaa tcgatgatca tctaacatat catcatgatg gttttactgt
     8281 agaggcgcat ttacttaatc atggtattcc atcatatgga tatagagtaa tggcacctga
     8341 aactacaggt acaatcaatg ttgaagcgtt aaaaaacatt ggtcttgaac caggtcctaa
     8401 atatcaagaa gtaaaatctc atgatacatt tgaacataat ggtcaagttt atcaatcaaa
     8461 agattttaga ggtgagtcca aacagggtcc agttgtagca atttttggtg atacaaaacc
     8521 atgttctaat gaacgtgtga tttctagaga tgctgatgtg atggtacatg aagcgaccta
     8581 tatagatggt gaaaagcatt tagccaacaa ttatcatcat agccatattg aagacgtatt
     8641 tgcattgata aaagaagcca atgttaaacg tacgttgatt acacatttga gcaatcgcta
     8701 taatacagaa gatataaatg aaatttatca aacattgata caaaatgaag atacacctaa
     8761 ttttaatttt gtaaaagatt ttgatagttt taaaatctaa tgacaaagta ataaacaatg
     8821 cttgccaaat aacaataaat tataaaaata acccgtccaa ctatgtatgc gatgatgcat
     8881 acatgtgttg gcgggtttgt ttttattggt cttctatatt agaaagttca atactacggt
     8941 cgacggcagc atttagacaa tcttcgaaaa tagatactaa atcatattgt gacaatgtat
     9001 caaggccagc ttgtgtcgta ccaccttttg aggtaatatt ttttcttaat tgagccatgc
     9061 tcaaatctga acgttcaatc attttacttg tacctataat aaggttgcgt atagattctt
     9121 caacttgttc tttttctaga ccaagtttcg taccagcttt aacgtattgc tcgaatacat
     9181 gatataaaaa tgctgggccg cttccggtga tagctgttac ttgatgtaaa tgatcttctg
     9241 atacttcaat tacagaacca aatgctttaa ctaaatcgtt aatttcatct ttagatttag
     9301 ggtcaaagtt gtttgaaaaa ctaatgccag taacagagtg tccgacttgc gcatttgtgt
     9361 ttggcataat tctagcaact ggattttggc attctaattg ttgtttaata taatcaatcg
     9421 gaatacctgc cataattgaa atgaagcaat tgtcttttgt gatatgtggt ttgatgcgtg
     9481 ttgctagagc atcaaagtca tgtggtttgg tacctaaaaa aacataatct gcatctttta
     9541 ataatgtcgc atcatcataa ctatagttaa cacctagttt ttcagcgaat gcttttaaag
     9601 cttgttcatt agatttattt gttaaatata tatcattggc atctaagttg cttgagttaa
     9661 taattcctgt aaatatagct tgtgccatat taccagctcc ataaaatacg agtttcatta
     9721 acgtcacatc cctttagttt ttgatttgta tgttatattt tcttattatt gtatatatat
     9781 taggaaagat aatcaacttg tctcaattat gtaaagttga taaattgcat tctatatgat
     9841 tgaaaaggaa ggtcagaaga tgaaaggtca acattttata gtaactggag gaacaagtgg
     9901 acttggttta tctatcgtcc gtaagttact tgataataaa gtgcgtgtaa cattgttggt
     9961 acgtgatgtt gataaagcaa ctagaatctt tgaacaggaa atcgggaaaa ctataaatgt
    10021 tattccttgt gacttaaatg atatgaaatc gatacaggca ctgaaatttg aaaacaatac
    10081 atcattcgat ggttttattt atagtgcagg tttaggttat ttcaaatcta tttcagaaca
    10141 tagtgtttca gaaatgattg aaacgtatca gcttaattta attagtttta atgttttata
    10201 tactgtgttg agaccatatt taacatctaa cgcacatatt gtaggtattt ccagtcaagc
    10261 tgcatttttc acacaagcaa atgctgcaca ttatggggca tcgaaagcag ggttttatgc
    10321 attaatgaat gctttaagac tagagtcacc aaacttacat atcatgacag ttaatgttgg
    10381 cccgattgat acaccttttc atcaaaaagc cgacccatca atgaagtatg ctaaaaagat
    10441 ggggaaaatt atgcttgatg ctaatcagct tgcagaagat atcatttatg gcattaaaac
    10501 aaaacaatta gaaattaatc ggcctaaatg gatgcatcat gcgttgaaaa tgtatcaaat
    10561 tgcgcctaga ttttttgaac gatgtttccc taaattattt aaaaataaag cataaatagg
    10621 tgggatggca atgaactgtc aatcggagag tgtggtaaga ctttgtgtac gatatgcgga
    10681 acaactatca gtatttgaag aatttacagt gctggatatt ttaagcgata tttctgtaga
    10741 ccaattttca gacagtacat tatattatac ttgtgaaaaa ttcaaactac ttgtgttgca
    10801 aggaaatgtg ctgggtgtac aagtaataac taataatgat gaatcaatat gcgaagtgaa
    10861 atatagaaag atgttttaat aaaattatta aaagatgaag tggcagaagt tagcttaagg
    10921 cactgccgca gggcgaaatt actaaatact gatgtgtgag gacaacgctt taatttctat
    10981 cataagtgag taatcttaat cataaattat agcgaatttt acataaacaa cagcagtaag
    11041 gcatttttat aatgaaacgc ctcatactgc tgtttttatt actgaaaatg acttctacac
    11101 taaatgattg gtatattcca aatcctttac acgagctctt gctgttttga tttgatctaa
    11161 actaacttta gcatgatagt tatcaatatt ttcttttaat tgatcgacac tgcttgcacc
    11221 tacaatgatg gaaccaagca cgtcatgtga tgttaagtaa ttaaacgtta aagcagacaa
    11281 attactttct atttctttaa tagaagctat agtctcgccc aattcatcat gtgaataatc
    11341 aaagatacca tctttgaatt tattgtcgag cacattaaca ctatttgaag ttaataatcc
    11401 tttagacaca ggtcctcttg ccaatacttt aacatcattg ttgtgaattg catctaataa
    11461 tgattctgga cgattatcaa tcaaattgaa ttgagacatt atcgtttcga tttgactatg
    11521 ttttaaataa taatcaatta catttgggcg aatagaagaa ataccgtaag cacgtataat
    11581 tccttcttgt ttcaattcat caaatgcact tattgtttgg tctaatgggt catcaatggt
    11641 tccgccatga agttgatata aatcgatatg atcaatacct aaacgcttta gtgaaccttt
    11701 aaccgcctct ttaatatagg atttactcgg atcccatgtt gtactgccat cttttgttaa
    11761 acgattgcct acttttgtac caatgacaat atcatcgcgt tgttgatatt taagaagtgc
    11821 cttaccaaca actgattcat ttataccttt atcgtacatg tctgctgtat caaaataagt
    11881 gatatcattt tcaatagcac aatctataat ttgttcggct ttttttaaat ctgtacctaa
    11941 actcatgcaa cctagaccta attcagataa tgcaatacca ctttttaata tatttttttg
    12001 catgtctgtt ctcctttcga tacactttag ctaacaagca tagtgtatca atatataata
    12061 tgaatttaaa ggaacgtgat tataatggat ttaaatgaaa aaacaattga tcgaacagtt
    12121 atttataatg gcaaaattgt agatgtagaa attcatacag tgacattacc aaacggtgaa
    12181 acgtcaacaa gagaattagt ttatcataat ggtgcagttg ctgtttgtgc agtgacacct
    12241 aaaaaagaag tcgtattagt gaaacagtat cgtaaaccag tagaaaaacc attgctagaa
    12301 attccagctg gtaaattaga agatgatgaa gatagagtcg aagcggctaa gcgtgaatta
    12361 gaagaagaaa caggatatat tgccaaagag ttaacacatg ttgtagatat gtatggttca
    12421 ccaggttttt gtgatgaaca attatcaata tattttacgg ataatttaca agaaggtaca
    12481 gttcacttag atgaagatga atttgtcgaa gtcattaaag ttccgattga gaatgttaaa
    12541 tcgatgctaa tgaataaaga aatagaagat gcgaagacta tcattgcatt gcaacaccta
    12601 ttattaaatt ataatcattc taaataacaa atgagaaaag cttgcatttt attgagaaaa
    12661 ctgttagttt taattgtgaa gtttgaaata atttgtaatg attttaatta ttagtagggg
    12721 agtggacatc gttggaagaa cgattaaatc gcgttaagca acaattacaa caatcatcat
    12781 ataagctaac gccacaacgc gaagctactg ttagagttct aattgaaaat gaaaaagatc
    12841 atttaagtgc tgaagatgtg tatttgaaag taaaagacaa agcaccagaa attggcttgg
    12901 cgacagtata cagaacgtta gagttgttag ctgaactaaa agttgtcgac aaaattaact
    12961 ttggtgatgg cgtcgctcgt tttgatttaa gaaaagaagg cgcaaaacat ttccaccatc
    13021 atttagtatg tatggaatgt ggtcgtgtag atgaaatcga tgaagatttg ttaccagaag
    13081 ttgaaaatcg agttgaaaat gagttcaatt ttaaaatttt agatcatcgt ttaactttcc
    13141 atggtgtgtg tgaaacgtgc caagctaaag gtaaaggata gtaaattgcg taggttaaat
    13201 taaccttcgc tttttttaga ggtgtggtta tggaaacaat tattgaagaa tatttgcgtt
    13261 ttatacaaat tgaaaaagga ctaagttcta acacaattgg tgcttataga cgtgatttga
    13321 aaaagtatca agattatatg actgaacatc atatctcgca tattgatttt atagatcgac
    13381 aattaattca agagtgtttg gggcatttaa tagaccaagg gcaatctgct aaatctattg
    13441 cgcgatttat ttcaacaatc cgtagttttc atcaatttgc tataagagaa aaatatgcgg
    13501 ctaaagatcc aacggtatta ttagattcac caaaatatga caaaaaattg cctgacgttt
    13561 taaatgttga cgaagtgttg gctttattag aaacgccaga tttaaataaa attaatggtt
    13621 atcgtgatcg tacgatgtta gaacttctgt acgcaacggg aatgcgtgta tctgaattga
    13681 tacatttaga gttagagcac gtgaacttaa taatggggtt tgtacgcgta tttggtaaag
    13741 gcgataaaga aagaattgta ccattaggcg acgcagtcat tgagtactta actacttata
    13801 ttgaaacgat tagaccgcaa cttttaaaaa agactgttac cgaagtctta tttttaaata
    13861 tgcatggtaa acctttatca cgacaagcaa tatggaaaat gattaaacaa aatggtgtaa
    13921 aggcaaacat taaaaagacg ctaacgccac atacgttgcg ccactctttt gcgacacatt
    13981 tattggaaaa tggcgcagat ttaagagcag tgcaagagat gttaggtcac tctgacatat
    14041 ctactaccca actctataca catgtttcga aatctcaaat tagaaaaatg tataaccaat
    14101 ttcatcctag agcataaagt gaaccataac tcaaaagtca caatacacat gactaaaaat
    14161 gtctgtgcta ttgtggcttt tttaaattgg ttgattaatt acgtctatgt tttcttaatt
    14221 gaatcgcttc ttcttttgct gcaatcactt ctgaacgatc acgccgcatg tgatggtcta
    14281 caataaaagg atctgttgct gtttcgtgat tataatcata atctgaatag ttggccttga
    14341 tgatgcgttc aaagtctgga gttattggta atataacaga tgaaaaaggc tttgctgcat
    14401 tcaattttgc aatctgttgc tcaattaaca actgataatc atttaaatta aggtataatg
    14461 catctctatc tttagcattt tgtattattt ctttagattt ataaaaagac ttataggcgc
    14521 cttttaaatt attgcggcga taatggtaac aagcagttgc aaacaagatt aaactaacaa
    14581 ctgcatcttg cttactgtag ttattttcag ctttccatgc atcttctaaa atgtcatgac
    14641 ataggaaata atgttgctta gtatgaaatt gataatagaa atttatcagt gcctgttgca
    14701 ttttgttatc accccaattt aaaagtaagt tattttcatg ctataatatt ttagagaatt
    14761 atgcacatat gacgcaatac gaggtagata ttatgtatga agttaaatta gatgctttca
    14821 atggaccatt agatttatta ctgcatctta tccaaaaatt tgaaatagat atttatgata
    14881 ttcctatgca agcattaaca gagcagtata tgcagtacgt tcatgcaatg aaacagcttg
    14941 aaattaatat tgcaagtgaa tacctagtat tagcgtcaga actcttaatg attaaaagta
    15001 agatgctatt accacaatca acatcagata tggattttga tgatgaccca cgggaagatt
    15061 tagttgggcg tttaatagaa tatcaaaatt ataaagaata tactgctatt ttaaatgaca
    15121 tgaaagaaga aagagatttt tattttacaa aaagaccgac agatttatct catttggaaa
    15181 cagatgaatc ttgggatcca aatcacacga ttgatttaac agaattaatt gtagcttatc
    15241 aaagagttaa aaatagagtt gagttaaata cacctaaatc tgttgaaatc cgaaaagaga
    15301 catttaccat tcaacaagct accgaacaag tgacatcgag attgaaagat aaagatcatt
    15361 ttaacttttt tagtctgttt acgttttctg agccaattga acaagtagtc actcactttt
    15421 tagctatttt agagatgtca aaggcaggaa taattaatat tgagcaacaa cgtaattttg
    15481 aagatattaa cattgttaga ggagtgaact accattttgg ataatcatgg tatattagag
    15541 tcgctattat ttacagctgg cgatgaaggt ttagatgaaa aacagctatt agaaatttta
    15601 gatatatcga aagaccaact cgtagaatta attgaaaatt attcatcaca tggattaata
    15661 atacaacgat ttggaacgac gtatgtttta acgactaaaa aagaagcggc aacgtacatt
    15721 gaacaattaa ttgaacaaaa gtcacaaatg aaattatcac aagcagcaat ggaagtacta
    15781 tcaattattg cttataacca accattatca agaagtgata ttgaattaat tcgtagtatc
    15841 aattcagatg gtgcagttaa aacattgatt gccaaaggac tagttgaggc taaagtggtc
    15901 aatgaacagc gtagccaaca attaatcact actgatttat ttttaaatgt atttggtatt
    15961 tccaatatag aagatttgcc gacaactgaa gaagacgatg aagaaatgga tgcttttttc
    16021 agtaatctag tcaatcaaaa aggagaaaat aatgactaaa gaattagaac gattacaaaa
    16081 acgtatagct aatagcggtt atacttcaag acgtaaggca gaaactttaa tttctgaagg
    16141 aaaagtgaaa gtgaatggta cgactgtcac tgaattagga acaaaagtaa agccatcaga
    16201 tacgattgaa gttgaaggta ttaaaataga actagaagac aaaatttaca ttttattcca
    16261 taaaccaact caagtgataa caagtgtatc agatgataga ggacgtacag tggttactga
    16321 ttactttaaa gatattgatg ctcgtattta tcctgttgga cgcttagact atgatacatc
    16381 tggattgtta ttattaacaa atgacggtga atttactaat ttaatgacac atccaagata
    16441 tcaaattaag aagaaatatg tcgcaaaatt aaaaggttat ttaatgagag aagaagtgaa
    16501 agcgctagaa aaaggtattg aattagaaga tggtatgacg caaccagctg aagtgaaagt
    16561 taagaaacaa gataaagata aaaacacaac attggttgaa attacaatta cagaagggcg
    16621 taatcgtcaa gtcagaagaa tgttcgaaca ttttggtcat caagtgacga aactgtctcg
    16681 tattgaatat gggcctttaa atgttgtcgg tttgaatgct ggtgaaggac gtgtattgac
    16741 gcctcatgaa gtaaaagtga tgcgtcattt agcagaacat gggaaataat taaataaaat
    16801 atgtatttat cacaaagttt gagaattttt tcacaaaatt ttaggttttt aataactgta
    16861 tgcgctttcc tgtgatataa tacttttgta aagaaaagca tgtgtgggag gtatgacctg
    16921 tatgtcgaac gaaatactta tcgtagatga tgaggataga atcagaagat tacttaaaat
    16981 gtatttagaa agagaatctt ttgaaatcca tgaagcaagt aatggccaag aggcttatga
    17041 acttgcaatg gagaataatt atgcttgcat actactagat ttaatgttgc ctgaaatgga
    17101 tggtatccag gtggcaacta aattgcgtga acataaacaa acaccgatta ttatgttgac
    17161 tgctaaaggt gaagagacaa accgtgttga aggttttgaa tctggtgcag atgattatat
    17221 cgtcaaacca ttttcaccaa gagaagtagt cttaagagtt aaagcacttc taagaagaac
    17281 gcaatctaca actgtagaac aaagcgaacc tcacgcacgt gatgtgattg aatttaaaca
    17341 tttagaaata gataatgatg cacatcgcgt acttgctgat aatcaagaag ttaatttgac
    17401 tcctaaagag tacgaattat taatatattt agctaaaaca ccaaataaag tatttgaccg
    17461 tgaacaatta ttaaaagaag tttggcatta tgaattctat ggtgatttaa gaacagttga
    17521 tactcatgtt aaacgactta gagaaaagtt aaaccgtgtg tctagcgaag ctgcgcatat
    17581 gattcaaaca gtctggggcg ttgggtataa atttgaggtt aaatctaatg atgagccggc
    17641 taaatagtgt cgtaattaaa ctgtggttaa ctattatttt aatagtgacg acagttttaa
    17701 ttttattaag tattgcttta attaccttta tgcaatacta tttcacacaa gaaactgaaa
    17761 atgccataag agaagatgct agacgtataa gttcactggt cgaacaatca cataataaag
    17821 aagaagcaat aaaatatagt caaacattaa ttgaaaatcc tggtgggttg atgattataa
    17881 ataataaaca tcgtcaatca acggcttcgc tttctaatat taaaaagcaa atgttgaatg
    17941 aagtagtcaa caacgaccat tttgacgatg tgtttgataa aggtaaatct gttactcgaa
    18001 atgtaacgat taaagaaaag ggctcatctc aaacatatat tttgttaggc tatccaacaa
    18061 aagcacagaa gaatagtcat agcaaatata gtggagtttt tatatataaa gacttgaaat
    18121 caatcgaaga tacaaataat gctattacga ttatcactat aattacggct gttattttct
    18181 taacaattac aacagtcttt gcgtttttct tatcgtcaag aattacaaaa cctttaagac
    18241 gcttaagaga ccaagctaca cgtgtatctg aaggggatta ctcttataaa ccttctgtca
    18301 caacgaaaga tgaaattggt caattatcgc aggcatttaa tcagatgagt acagaaatcg
    18361 aagagcatgt cgacgcatta tccacatcta aaaatattag agacagctta attaactcta
    18421 tggtagaagg tgtcctaggt attaatgaga gtcgacaaat tatcttatcg aataagatgg
    18481 cgaatgatat tatggacaat attgatgaag atgacaaagc tttcttatta agacaaattg
    18541 aagatacttt taaatcaaaa caaactgaaa tgcgtgattt agaaatgaat gcacgattct
    18601 ttgttgtgac cacaagctat atcgacaaga ttgaacaggg aggtaaaagt ggtgttgttg
    18661 tgacagttcg tgatatgacg aatgaacata accttgatca gatgaagaaa gatttcatcg
    18721 caaatgtatc acacgaattg cgtacgccga tatctttact tcaaggttat actgaatcaa
    18781 ttgtcgatgg tattgttaca gaaccggatg aaataaaaga atcgcttgcc attgtccttg
    18841 atgaatcgaa acgtttaaat cgtttagtta atgaattgtt aaatgttgcg cgcatggatg
    18901 ctgaaggatt atcagtaaat aaagaagttc agcctattgc agcgttacta gataagatga
    18961 aaattaagta tcgtcaacaa gctgatgatt taggtctaaa tatgactttt aattattgta
    19021 agaaacgtgt ttggagttat gacatggatc gaatggatca agtactaacg aacttaattg
    19081 ataatgcatc acgttatacg aaacctggtg atgaaattgc aattacttgt gctgaaaatg
    19141 aaagcgaaga tattttatac attaaagata caggcacagg cattgcacca gaacatttac
    19201 aacaagtatt tgatcgtttt tataaagttg atgcagcgag aacgcgaggt aaacaaggta
    19261 ccggtttagg tttgttcatt tgtaaaatga ttatcgaaga gcatggtggt tccattgatg
    19321 ttaaaagcga attaggaaaa ggcacaacgt ttattatcaa actaccaaag ccggaataaa
    19381 actgaatata gttatttaag aacgcatgtt attgattaga gactctaatt tatagcatgc
    19441 gtttttaatt gaggtgggta attttttaag tggattagga attagaactt ttgcgcattt
    19501 caactattaa atatattgct aatttatata aaaatataaa gtttgataaa gttatttatt
    19561 tgattataaa aatagggtaa aatatagata tattgtaata attaaattat tcgaggtgtc
    19621 atatgaaaaa gttcattgga tcagttttaa ccacgacatt aatcttaggg ggatgttccg
    19681 cgatggaaaa tgaatcaaat aaagacacaa acacagaaac aaaatcagta ccagaagaaa
    19741 tggaagcttc aaaatatgta ggacaaggct tccaaccgcc tgcagaaaaa gatgcgattg
    19801 aatttgcgaa gaagcatcgt aaagaatttg aaaaagtagg tgaacaattc tttaaagata
    19861 actttggact aaaagttaaa gctacaaatg ttgtaggtaa agatgatggt gtagaagttt
    19921 atgtgcattg cgaagatcat ggcattgtat ttaatgcaag tctacctttg tacaaagatg
    19981 ccatccatca aaaaggatca atgcgtagta atgacaacgg tgatgatatg agtatgatgg
    20041 tgggtacagt gctgagtggc tttgaatatc gagcgcaaaa agaaaaatat gataacttat
    20101 ataaattttt caaagaaaat gaaaagaaat atcaatatac aggttttact aaagaagcaa
    20161 ttaacaagac gcaaaatgtt ggatataaaa atgaatattt ttatattaca tactcttcta
    20221 gaagtttaaa agaatatcga aagtattatg aaccattgat tcgaaaaaat gataaagaat
    20281 ttaaagaagg aatggaacga gctagaaaag aagtgaatta cgctgctaat acagatgctg
    20341 ttgctacact tttttctact aagaaaaact ttactaaaga caatacagta gatgatgtaa
    20401 tcgaactaag tgataaatta tataatttaa aaaataaacc agataaatct acaatcacaa
    20461 tacaaatagg gaaacccact attaatacta agaaagcctt ttatgatgat aatcgtccaa
    20521 tagaatatgg ggtgcacagt aaagatgaat aaaattaatg atagggattt aaaagaatta
    20581 agtagttact gggtttatca aaatattgat ataaaaaaag aatttatagt taatggaaaa
    20641 aggttcaaac aaatagatag ttataatgat gataagaata gtaatttgaa tggtgctgct
//
LOCUS       DAHHRR010000037        20379 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.037, whole genome shotgun sequence.
ACCESSION   DAHHRR010000037 DAHHRR010000000
VERSION     DAHHRR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20379
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.037"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            11..1102
                     /locus_tag="NBI42_001206"
     CDS             11..1102
                     /locus_tag="NBI42_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1777633.1"
                     /translation="MIDFFKDNQRIERFSFNNNKVHMKETFNIDNKVCYQVFYDEKGF
                     PYISRNINASNGAVGKTYLIVCKKEFKNNLALCVYYLEKLIKDNKNSIMICDGPGSFP
                     KMFNTKHKNAQKYGVIHVNHHENFDDSGAFKKSEKFIIENADNINGVIVLTDAQRLDI
                     LQQFDVKNIFTISNFVKIHKAPKQFQTEKIVGHISRMVPTKRIDLLIDVAELVVKKDE
                     TVKFHIYGEGSVKGKIVKKIIDKKLENHVLLKGYTTTPQKCLEDFKLVVSTSQYEGQG
                     LSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMADKILHLVNNDVLAE
                     EFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(1191..1547)
                     /locus_tag="NBI42_001207"
     CDS             complement(1191..1547)
                     /locus_tag="NBI42_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCD1777634.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            2035..2994
                     /locus_tag="NBI42_001208"
     CDS             2035..2994
                     /locus_tag="NBI42_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1777635.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKAVGIADDGSSKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADISRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(3039..3170)
                     /locus_tag="NBI42_001209"
     CDS             complement(3039..3170)
                     /locus_tag="NBI42_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777636.1"
                     /translation="MRYVTMRQFIKRIVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(3234..3449)
                     /locus_tag="NBI42_001210"
     CDS             complement(3234..3449)
                     /locus_tag="NBI42_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCD1777637.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            3614..4165
                     /locus_tag="NBI42_001211"
     CDS             3614..4165
                     /locus_tag="NBI42_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1777638.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEKIKQYGTPLP
                     VKEAKDDEYYIETIATFEAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYQ
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(4217..5155)
                     /locus_tag="NBI42_001212"
     CDS             complement(4217..5155)
                     /locus_tag="NBI42_001212"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCD1777639.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGSAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            5337..6698
                     /locus_tag="NBI42_001213"
     CDS             5337..6698
                     /locus_tag="NBI42_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCD1777640.1"
                     /translation="MATGVLEDDIVKEIYDSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHQFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAIDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            6685..8358
                     /gene="menD"
                     /locus_tag="NBI42_001214"
     CDS             6685..8358
                     /gene="menD"
                     /locus_tag="NBI42_001214"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCD1777641.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLKVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            8345..9148
                     /gene="menH"
                     /locus_tag="NBI42_001215"
     CDS             8345..9148
                     /gene="menH"
                     /locus_tag="NBI42_001215"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCD1777642.1"
                     /translation="MTHYNYYEANVETNQVLVLLHGFLSDSRTYHNHIDKYTNICHVI
                     TIDLPGHGEDQSSIDETWNFDYITTLLDRILDKYKDTSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPQKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            9141..9962
                     /gene="menB"
                     /locus_tag="NBI42_001216"
     CDS             9141..9962
                     /gene="menB"
                     /locus_tag="NBI42_001216"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCD1777643.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDNQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLDKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(10201..10530)
                     /gene="sspC"
                     /locus_tag="NBI42_001217"
     CDS             complement(10201..10530)
                     /gene="sspC"
                     /locus_tag="NBI42_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HCD1777644.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDNTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(10568..11749)
                     /gene="sspB"
                     /locus_tag="NBI42_001218"
     CDS             complement(10568..11749)
                     /gene="sspB"
                     /locus_tag="NBI42_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HCD1777645.1"
                     /translation="MNSSCKSRVFNIISIIMVSMLILSLGAFTNNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITILT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(11831..12859)
                     /gene="sspA"
                     /locus_tag="NBI42_001219"
     CDS             complement(11831..12859)
                     /gene="sspA"
                     /locus_tag="NBI42_001219"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HCD1777646.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANAFSSKAMDNHTQQTQT
                     DKQQTPKIQKGGNLKPLEQREHANVILPNNNRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDINFANDDQPNNPDNPSNPDEPNNPDNPNNPDNPNNPDEPNNPDNPDNPDNGDNN
                     NSDNPDAA"
     gene            complement(13387..14541)
                     /locus_tag="NBI42_001220"
     CDS             complement(13387..14541)
                     /locus_tag="NBI42_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1777647.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYVDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKLTPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFE
                     AIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(14737..15747)
                     /locus_tag="NBI42_001221"
     CDS             complement(14737..15747)
                     /locus_tag="NBI42_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCD1777648.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIIYVIHPINADWLLGSSP
                     YTSLENEGQSVFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRSSQFK
                     DS"
     gene            15900..16319
                     /locus_tag="NBI42_001222"
     CDS             15900..16319
                     /locus_tag="NBI42_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1777649.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            16346..16456
                     /locus_tag="NBI42_001223"
     CDS             16346..16456
                     /locus_tag="NBI42_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777650.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(16526..20299)
                     /locus_tag="NBI42_001224"
     CDS             complement(16526..20299)
                     /locus_tag="NBI42_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCD1777651.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTKPVAKTTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKAAAPKAATTTAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTPSTGKLTVAAN
                     NGVAQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFG
                     WVKEGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTPKQVAGSVSGSGNQTFKASKQ
                     QQIDKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPKPSTPTTNNKLTVSSLNGVAQIN
                     AKNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPSLIGWVKQGDV
                     IYNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSI
                     YLYGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYAVTKPQTTQTVSKIAQVKPN
                     NTGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLN
                     VQNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDV
                     YLYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKY
                     SLKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQV
                     AQIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISI
                     DKINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNN
                     KVVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAG
                     QNTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
BASE COUNT         6933 a         2949 c         3583 g         6914 t
ORIGIN      
        1 aactgaaaaa gtgatcgatt tctttaaaga taatcaacga atagaacgat ttagttttaa
       61 caacaataaa gtgcatatga aagagacatt taatatagat aataaggtgt gttatcaagt
      121 attttatgat gaaaaaggat ttccatatat ttctaggaat atcaatgcaa gtaacggcgc
      181 agttggtaaa acatatttaa tagtatgtaa aaaagagttc aaaaataatc ttgcactatg
      241 tgtttactac ttagaaaaac ttatcaaaga taataagaat agtattatga tttgtgatgg
      301 accaggtagt tttcctaaaa tgtttaatac aaaacataaa aatgctcaaa agtatggcgt
      361 tatccatgtt aatcatcatg aaaattttga tgatagtgga gcatttaaga aaagtgagaa
      421 atttattatt gaaaatgctg ataacataaa tggtgttatt gtgttaacag atgcacaacg
      481 tttagatatt cttcaacaat ttgatgtaaa aaatatattt actattagca attttgtgaa
      541 aatacataaa gcacctaagc aatttcagac tgaaaaaatt gttggtcata tttctagaat
      601 ggttccaact aaaagaatag atttacttat tgatgttgct gaattagttg ttaaaaaaga
      661 tgaaacagtt aaattccaca tttatggtga agggtcagta aaaggaaaaa ttgtgaaaaa
      721 aattatcgac aaaaaattag agaatcatgt tttgttaaaa gggtatacaa ccactccaca
      781 aaaatgttta gaagatttta aattagttgt ttctacatct caatatgaag gtcaagggtt
      841 aagtatgata gaagcaatga tttctaaaag gcctgttgtt gcctttgaca tcaaatacgg
      901 gccaagtgat tttatagaag ataataaaaa tggttattta atagaaaacc ataatattaa
      961 cgacatggct gataaaatac ttcaccttgt taataatgat gtattagcag aggagtttgg
     1021 ttcgaaagcg agagaaaaca ttatagaaaa atattcaacg gaatcaatat tagaaaaatg
     1081 gttaaatctt ttcaatagct aaatgaaaaa atcctaggta ttcatgagtg cttaggattt
     1141 ttacgtatga tatatacaaa aaaagagagg atagtcctct caaataatag ttaataaaat
     1201 tgtagccatt tcttccagct gtaactacta atacaagcta agttcataac gatatgtatg
     1261 atttcaaact cataaccact tacatatcct cttgtaagtt taagtgtgaa gattgagcct
     1321 aacataacac aaatataaaa aacattaaca taaggcgtta aaaagccgat aataagtgct
     1381 aaacctccaa caacttcacc aattgacatc aatactgcga tggtaggtgg taaacctaaa
     1441 gagcctaaga agtgaattgt tccatctaaa tcgacaaatt tcaaagtacc gtgcactaag
     1501 aatgatgttg ctaacatcca tctaattaaa agcataccta atttcatcat gtcacctcca
     1561 acattcgtat ttttaaaaca tagtccaaat tatatttgta aacatgtgga aagtaaagat
     1621 tttttataag attaattatt aaatatttat taaatattct attatattga acaattgttg
     1681 tagaaagatg tttaagtcat cagttacttt ttgtatttat aaataaagat tagtacagca
     1741 tagtgcaaag atgataggga aatgaattaa ctattgcatc gctatttatg aatcgtaaac
     1801 actattatga aattgcaaga gtagtatgga taattagaat aatggtttga gataaatgag
     1861 tgttattaac cagtgcgtca tcttcaaaat tagaggccat ttgctgttta taataagaag
     1921 atttgataat caactatgat aagaaaaaat aataatcccc taaatacatt ataaacaaat
     1981 taaatgataa aataataata tagatttcta ctaaaattgt ttggaggtaa atgtgtgaat
     2041 aggaatatcg ttaaactagt tgtgtttatg ctaatcttag ttgtagcagt agcgggttgt
     2101 ggtcaaaaag atactgaaga gaaaactgaa atgacgacaa taaaagatga attaggaact
     2161 gaaaaaatta agaaaaatcc taaacgtgtt gttgtattag aatatagttt tgctgattat
     2221 ttagcagcat tagatatgaa agctgttggt attgcagatg atggcagcag taaaaatata
     2281 acaaagtcag taagagataa gattggggca tatgaatcag ttggatctag accgcaaccg
     2341 aatatggaag tgataagtaa attaaaaccg gatttgatca ttgcagatat tagcagacat
     2401 aagaaaatca aatcagaatt gagcaaaatt gctccgacaa tcatgttagt tagcggtacg
     2461 ggagattata atgcaaatat tgaagcattt aaaacagtcg ctaaagcagt aggcaaagag
     2521 aaagaaggcg agaaacgtct ggaaaagcat gataaaatat tagcggagat tagaaagaaa
     2581 attgaacaga gtacgttaaa atctgcattt gcattcggta tctcaagagc aggtatgttt
     2641 attaataatg aagatacatt tatgggacaa ttcttaatta aaatgggtat tcaacctgaa
     2701 gtcacaaaag acaaaactac gcatgttggt gaacgcaagg gtggtcctta tatttattta
     2761 aataatgaag aacttgccaa tatcaatcca aaagttatga ttttagccac tgacggaaaa
     2821 acggacaaaa atagaacgaa attcattgat cctgcagttt ggaaatcatt aaaagctgtg
     2881 aaagataaca aagtttatga cgttgaccga aataagtggt tgaaatcaag gggtattatc
     2941 gcaagtgaaa gtatggcaga agatttagaa aaaattgcag aaaaagcaaa ataaaaatac
     3001 agcgccactc gtaatcatat aagagtggcg ctgtattttt aattatgttt attatctgtc
     3061 ggatgtgatg atttacctga aagtttattt cgaataaatt taattacata accgacaagg
     3121 attgttttaa caattctttt aatgaattgg cgcatcgtta catacctcat ttctctatat
     3181 gttacgaact atatacccat tcatatatgc tttttaaacg tcattgtcac aatttaattt
     3241 ttagggaata taatataacc atctttatct gcttttttag taaaaatgac aaaaatggca
     3301 tgtattattg agatgatggt agggatacct gtccagaaaa ataataagtg aaaaagacct
     3361 tgtccaaatt tatcagcata aaatttatga atacctaaac ctccaagaaa taatgcaaca
     3421 ataacataaa tggctttatt gactttcatt tgtaaatcct ccttaactat aattctactt
     3481 aaattcgttg tgaaaaccaa tatttctaac tttagaattt tcaaactttc taaaattata
     3541 ggtatatctt tttaaaataa gctagaattt ctatataata aatgttaata acgtaaaagg
     3601 gaatgatgac atagtgatac gtcaagcacg tccagaggac cgatttgata ttgcgaagtt
     3661 agtttatatg gtttgggatg atatggaatt agaattggta aagcatctac ctaaagacat
     3721 ggtattagat gcaattgaaa aaagctgtgt tgatgcaaca tatcgaactt tttatcagca
     3781 tattttagtt tatgaagtag aaaataaagt agcaggttgt attattagct atagcggtga
     3841 aaatgaattg aaatatgaaa aagcatggga actacttgac ttgccagaaa aaataaaaca
     3901 atatggcacg ccattacctg taaaagaagc taaagacgat gagtattata tagaaacaat
     3961 tgcgacattt gaagcatata gaggtagagg catcgcgaca aagttattaa cgtcattact
     4021 tgaatcaaat acacatgtta aatggagttt gaattgcgat attaataatg aagcagcatt
     4081 aaagttatat caaaaagtag gctttatatc tgatggacag attgaattat acaagcacat
     4141 gtatcatcat ttaattgtta agtaaaaaaa ctcgacagtt cgatgtaagt cgattgccga
     4201 gtagaatcat cttatattaa atgcctgcaa ataatgcact aatataaata cctaatgcat
     4261 ataataaacc gaaaaatgta tttgttttac cagcagcagc cattgctggc atcattgtag
     4321 gcggtgtatc attcttcttg aaacgtctga taactttaac aggcattggg aatgataaca
     4381 acgcaagtaa gtaaaataat gagccaccag gtttaataat gatcgtaagt acaataaagg
     4441 cataagcgat aaagtacatg attgccataa atgttaaaga agcattttta cctaatagaa
     4501 tgggtaaagt tttacgacca cttgctttat ctttgacacg gtcgcgaata ttgttagcca
     4561 tattaattaa accaatagtg attactatag gtacactcaa ccaaattaca taactttgaa
     4621 tattgccagt ttgaataaag aatgcaataa cgataataaa catacccata aatacgcctg
     4681 agaataattc accgaaaggc gtccatgaaa tagggaaagg gccacctgta tataggtaac
     4741 caacagccat acatactaat ccaactggta ataaccaaaa tgaagagtta gcagctaaaa
     4801 acaaacctaa tattgctgct aagatgtaaa atgcaatggc taatcgtagc acaagctctg
     4861 ggctcatacc gttgcgtaca atggcaccac caatgcctac agattcatga tcatcaaggc
     4921 cttttttata atcatagtat tcgttaaaca tattagttgc tgcttgaata agtaagcatg
     4981 ctagtaacat ggcaatgaat aggctgattt taatatgatc ttcgctacca agaaaatata
     5041 ttttagatgc tgctgaacca actaaaacgg gtactacgga agcagttaat gtatgaggac
     5101 gcattaaatg ccaatatttc ttaactgtag aatattgctg atattgatta ctcattgaat
     5161 acaaaacctc tttaaatcat attttttata acaaagatat tttaatagaa agtagcataa
     5221 aggtcaattg acgtgaaaaa ttgcaagata agtttaagca attcttgtaa cacaattatc
     5281 caaaaatgat acaatataat aatgagtgca aaaagacgaa gaaagaagtg aaatggatgg
     5341 ctacgggcgt attagaggac gatattgtca aagagatata tgacagctca aaggaatggg
     5401 tttcagttga agtgaaatta tcacagtcac tagacccgag cacattattt catctcactg
     5461 acaatgaggc aggagatcgc ttttatatgc gtttgaatga taatcgaacg tcatattttg
     5521 gctacaaagc aattcaatta ttcaaaaata attctaaaaa taaacaatct atttttaaag
     5581 actgggaaaa attaaaacat aacatcacat ttatacatcc gcaatctgag aaacatcatc
     5641 ttcgagttgt tggagggttc caattttcaa gtcataaatc agatgatgaa tggcgagagt
     5701 tcggactaaa tcattttgta ttacctgaag ttttaatttc aactgataat aatgggacat
     5761 ttttaactta tacagttaaa agggaaagtt ttactgttga ggcattgaac gatttaatgg
     5821 atttgttcaa caatatatcg gatatagatg tggacgagca aattggggaa attactagaa
     5881 atgaagatat ttataaagat gactggcgtc aacttgtagt agaagcaata gaatctatta
     5941 ataatgaaga aaaaattgta ctagcacgta gacggttaat aaagttcgat aaagatatca
     6001 gtattccata tattctaaag caagcatatt ctaaagaaaa aaacagttat atattcttgt
     6061 tagaatcaca agattctata ttcttttcac aaacacctga acaattaata aaggtcaata
     6121 ataaaatact atcgactaaa gctgtagcag gtacaattaa acgttctcaa gatgaggacg
     6181 aagatacaaa aaatgttgaa gcatttttaa aagataataa aaacttaatc gaacatcaat
     6241 ttgttgttga cagtatttta catgatatta aaccttatat cactgaatta cattatgata
     6301 agacgcctaa aattctaaaa aatgatcatt tatatcactt gtacactgaa ataaaggcgc
     6361 cactgaagga tgattcgtat attagtttaa ttgataattt acatccaaca cctgctttag
     6421 gtggctatcc aaaagaattt gcgatagatt ttattgaaca gaaagaattt ggtacacgag
     6481 gattatatgg tgcgccggtt ggctatatag atatatatga tgattgtgaa tttattgttg
     6541 caattcgttc gatgcttatt aagaaagcac aagcaacttt atttgctggg tgtggcattg
     6601 ttaaagattc tgatccagat agtgaattgg cagaaacgaa ccttaagttc acacctatga
     6661 tgaatgcatt aggagtcgat atgaatggga aatcataaag cagctttaac caagcaagtt
     6721 tttacatttg catctgagtt atatgcgtac ggcgtaaggg aagtagttat cagtccggga
     6781 tcacgctcaa cgccacttgc acttgcattt gaagcacatc caaatattaa aacatggata
     6841 caccccgatg agcgaagtgc agcgtttttt gcagttgggt taattaaagg cagtgaaaga
     6901 cctgtcgcta tattatgtac gtcaggtaca gcagcagcga attatacgcc tgcaattgct
     6961 gaaagccaaa ttagtagaat tccattaatc gttttaacaa gtgaccgtcc gcatgaatta
     7021 agaagtgtag gcgcaccaca agcgattaat caagtaaata tgtttaataa ttatgtaagt
     7081 tatgagttcg atatgcctat tgcggatgat agtaaagaga ccattgatgc aatttattat
     7141 caaatgcaaa ttgctagtca atatttatat ggaccacata aagggccaat tcattttaac
     7201 ttgccattta gagatccgtt aactcctgat ttgaatgcaa cagaattgtt aacttctgag
     7261 atgaagattt taccgcacta tcaaaaaagt atagatgcat cggcattaag acacatttta
     7321 aataagaaaa aaggtttaat tattgtaggg gatatgcagc accaagaagt tgatcaaata
     7381 ctgacgtatt caacgatata tgatttgcct attttagctg atcctttaag tcatttaaga
     7441 aaatttgatc atccgaatgt tatctgtaca tatgatttgt tgtttagaag cggcttagac
     7501 ttaaaagtgg atttcgtaat tcgtgttggg aaaccagtga tttctaaaaa gttgaatcaa
     7561 tggttaaaga aaactgatgc atttcaaata ttagtgcaaa acaatgataa gattgatgtc
     7621 tttccaatag caccagatat ttcatatgaa atatctgcga atgatttctt taggtcatta
     7681 atggaagaca cgacagttaa tcgcgtaagt tggttagaaa aatggcaatg cttagagaaa
     7741 aaagggcgta aagaaattaa atgttatttg gaacaagcta cagatgagag tgcattcgtt
     7801 ggtgaattga ttaagaaaac atctgaaaaa gatgcattat ttattagtaa tagtatgcca
     7861 atcagagatg tagataactt gttattgaat aaaaatatag atgtctatgc gaatcgtggt
     7921 gcgaatggta ttgatggtat cgtttcaact gcactgggta tggcagtgca taaacgaata
     7981 acattattga taggtgattt gtcattttat catgatatga atggactatt aatgtcaaaa
     8041 ttaaataata ttcagatgaa tattgtatta ttgaacaacg atggtggcgg tattttttca
     8101 tatttaccac aaaaagaaag tgcaaccgac tattttgaac ggttgtttgg cacaccgacg
     8161 ggattggatt tcgagtatac agctaagtta tatcaattcg attttaaacg ttttaacagt
     8221 gtttcagaat ttaaaaatgc cacattgtta tctgaaactt cgacgattta tgaattgata
     8281 acgaatcgcg aagataactt taaacagcat caaattttat atcagaaatt gagtgaaatg
     8341 attcatgaca cattataatt actatgaagc aaacgttgag accaatcaag ttttagtatt
     8401 actgcatggt tttcttagcg acagtcgtac ttatcataat cacatcgata aatatactaa
     8461 tatctgtcat gtcatcacta tagacttacc aggacatggc gaagatcagt cttcaataga
     8521 tgaaacgtgg aattttgatt atattacgac gttgttagac cgaattttag ataaatataa
     8581 agatacatca ataacattgt ttggatattc aatgggtggg cgtgttgcat tatattatgc
     8641 aattaatggt cacatcccta tatctaattt gatattagaa agtacgtcac caggtattaa
     8701 agaagaagca aatcaattgg aacgccgtct tgttgatgat gcacgtgcta aagtattaga
     8761 catagcaggt attgaattat ttgttaatga ttgggaaaag ttgccattat ttcaatcgca
     8821 actagaatta ccagttgaaa tacaacatca aataagacaa caacgattgt ctcaatcgcc
     8881 acagaaaatg gccaaagcat taagagatta tggtacaggt caaatgccaa acttatggcc
     8941 gcgcctgaaa gaaattaaag taccaacatt aatattagct ggagaatatg atgaaaaatt
     9001 tgtacagatt gcgaaaaaaa tggcaaattt aattcctaat agtaaatgta aattaatttc
     9061 tgctacaggt catacaattc atgtggaaga tagtgatgaa tttgatacaa tgatattagg
     9121 atttttaaag gaggagcaaa atgactaaca gacaatggga aacacttaga gaatatgatg
     9181 aaatcaaata tgaattttac gaagggattg ctaaggtaac aataaatcgc cctgaagtac
     9241 gcaatgcgtt tacacctaaa acagttgctg aaatgattga cgcattttca cgtgcacgtg
     9301 ataatcaaaa cgtttcagtt atcgtattaa ctggtgaagg tgatttagca ttctgttctg
     9361 gtggtgacca gaagaaacgt ggacatggtg gttatgtagg tgaagaccaa atccctcgct
     9421 taaatgtatt agatttacag cgtttaattc gtattattcc aaaaccggtt attgcgatgg
     9481 taaaaggtta tgctgtaggt ggcggtaatg tactaaatgt tgtttgtgac ttaacgattg
     9541 ctgctgataa tgctattttt ggacaaactg gtcctaaagt aggttcattt gatgcaggtt
     9601 atggttcagg atatttagca cgtatcgttg gacataagaa agcacgtgaa atctggtact
     9661 tatgtcgtca atacaatgca caagaagctt tagatatggg tctagtaaat acagtggtac
     9721 ctttagataa agttgaagat gaaactgtgc aatggtgtaa agagattatg aaacactcac
     9781 caacagcttt acgattcctt aaagcagcta tgaatgctga cacagatggt ttagctggtt
     9841 tacaacaaat ggctggggat gcaacattgc tttattacac aactgatgaa gcgaaagaag
     9901 gccgtgatgc gtttaaagaa aaacgtgatc ctgacttcga tcaattccct aaattcccat
     9961 aagttatttt gaatatgata ttaagtcact tgcgtcgttt gtttgcgatg acgagtggct
    10021 tttttatttt ataagaattt aaaaggggct tttaattaat tatcataaag ataagatagt
    10081 ttgagaaaaa ttaatagaga aagaataaat gttaaagatt attattacaa tgaaaaaacg
    10141 agctggtgag aaattgaatt ctcgccaacc cgctttatga agtgaaatca tttaaattag
    10201 ttatactaag cgctcataaa cgattggtcg cgaagtgcca ataccttgtt tggatgacgt
    10261 ttgcatgagg atatgctgag tatcatcata atcaagaata taaatgattt cttcattatc
    10321 aatagatgaa aatttaatgc gttgatgtgc cgtatctata aaaagaatat gatattgatt
    10381 gtgacttgta ttatcgccat gacgtataca aattgatttt tgtaattgat gattactcca
    10441 attaccaata aataaattga tatttgtttg ttctagatgt gtgagtttgg ttgtgtcgta
    10501 aactaaattt ataaattgta gttgatacat agttttacct gcctttaatc aatatctata
    10561 ttacttttta atagcctatc attgaaccat accagttata atcacgattg aatgataaat
    10621 gtaataggct tgaatctgca tcttggattg ataattctgt atcccaagga ttccagtaga
    10681 taagtttttc ttggtcatta attttagcat taccaacaac tgctagggca tgtcctaaat
    10741 gtgggtcatt agggttttga gatacacttt gtgcaagaat cataattcct acattatctt
    10801 ttgtaagttg atcaacttgt tcatatgatg gtacaccttc ttgataatga atatctctgc
    10861 cttgtgattt accgtattca atcatttgat tagggaatgt tgcacaatta ggaaggtctt
    10921 gctcacttac ttcagggtat aatgtacgca taatatcatg tgcattataa gtgtctgtat
    10981 ttttagttgc atttaataat gctgccatac tgaatcctgc acaccatgag ttatcgaatt
    11041 gttgttctct aattttgaag ttttttaatg tgttttcata ttgaacttga tcttcttgta
    11101 tcgcttcgtt ttcttcaact ttagtaggtg ttactgtatt ttttaactct tgtttcaatt
    11161 ttgctgaaac tgttttagca ctttcttttt cttttacatt accaggtagt ggcgtagctt
    11221 ttactaatct aactttgcca tcttcttcaa aataaaatcc tttttcatca gtaagaatag
    11281 tgatatttga atttttatct ttaatttggt ctaaatcttt agcgatgaag tttgaaattt
    11341 taacactgta attcatgtct tcttttgatt tgtttaaatc atctttattt ttagggctaa
    11401 gtgttaaagt ataaacaatt ttaccgtctt ttataactgg ataatagtaa ctattatctt
    11461 cttcaccatt aaatttataa attttaaaag cttcgccaag ttcatattta ccagtttttg
    11521 cattactttg tttatctaat gctttagcat aaccagcaaa ttgttgttgt gctagatctt
    11581 ttactttttg aggtactttg tcactcttaa cattaatttc tagctgttta gagtgtgaat
    11641 cagctttggc tttattgtta ttagtaaatg cgcctagtga taaaataagc attgaaacca
    11701 ttatgatgct tataatattg aatactctag atttacatga actattcata taataccctc
    11761 catttaagtc aaaatatttt gcgttgctaa gtctttgctt ctatttacta aatccaaatt
    11821 aagatgaagt ttatgcagcg tcagggttgt ctgaattatt gttatcgcca ttgtctggat
    11881 tgtctggatt atcagggtta tttggttcgt ctgggttgtt agggttatca gggttgttag
    11941 ggttatcagg attatttggt tcatctgggt tgctagggtt atctggatta ttaggttggt
    12001 catcgttggc aaaattgata tcttcaatat tttgttttaa gaagttgcgc acattttcgt
    12061 taataaatac tgcaccatta aattcatttg gtacaccgcc ccaatgaatt ccaatcactt
    12121 catttttctc attaaatact ggtgatccag agttgccgcc agttgtactt aaatcatatt
    12181 gcattgcttc accttttaag tacgttattt ttcctttact ttcccacatt gttgcgacag
    12241 gtttatcacc tggatatcct gttacagtaa tattttggtt aacttgtgtt tcagcattat
    12301 tactcattgt tgctggttta actacttcgc caatatgttt gttttgctca ttaggggaga
    12361 atttaactat tgctaaatca ccttcgcctg aatatttagt gatttgttca gcagtgaaac
    12421 caccatttgg ataattgtct tggttaattg cagaagggaa tgcttttaaa gcatgaggat
    12481 caccgtgcgt agcatctacg acgtgtttat ttgttaaaag agtatcttta cctacaacta
    12541 caccggaagc aataaatgta ccagtaggtg cttcaacttg aatataagtt acgggtgcat
    12601 aatgaccatt cgttgtatct gtaatttggt gacgattgtt atttggtaat ataacatttg
    12661 cgtgttcgcg ttgttctaat ggtttaaggt taccgccttt ttgaatctta ggtgtttgct
    12721 gtttgtctgt ctgcgtttgt tgtgtatgat tgtccatagc ttttgaagaa aacgcatttg
    12781 ctgctggaga actcacaagt gtcgctgttg tcaaagttgc aacgaataaa gaactaactt
    12841 ttaaaaattt acctttcatc taaaaacctc caaaaaattt atttacaagt taaatataac
    12901 actaaaaatt tttaagtcaa taagaatata taaataaatt aaaaagtttt tgtaaatatt
    12961 agagtttaca aaaacattaa gggaattaaa attgaattgc attacacttt attcgattga
    13021 gttaaattga taaggttgtc aattgtttgc atttatctaa aagaataaat aagaaaggaa
    13081 ttgagagcga agggaatgta tttgtttata ataataaata gtaacgtttg aataataatt
    13141 gtttggccaa aggtagatta atatgagtgg tgatatatta tttgagtgct taagtttcga
    13201 agaacaatta taagttagca tattaaaagc ttttgataga atcattttga gtgaggtgtt
    13261 taaaggagag gaatataact cggtagtatt tacagtctct ttagagtatt atttaaaact
    13321 tatagaatat aaaaaactga acgtactatg aatttagttc acgacgtcca gttcaacata
    13381 tcatttttat ttagtattta aatatttagc aagtctcttc ataccttctt ttaaaacatc
    13441 catttcataa gcataggaaa tccttacaaa tcctttaccg aattctgtaa aggatgagcc
    13501 tggaacgatt gctaaatgtg ttgattcaag taaatcaaca caaaattcga aatcgtcatc
    13561 ggtgatatgc ttaatacttg ggaaaatata aaacgcgcct tcaggttgag cggtaatctc
    13621 aaaacctaat ttagttaatt cggatactaa ataatttctt cgttctacat aagcttcatt
    13681 catatattta ggagcttcta atccttcgtt aagtgctgtt atacatgcta tttgagctgg
    13741 aacattggca caaatacaat tataggcgtg cataaatgtt aatttatcaa tcaaatattg
    13801 aggtcctaat agaaaaccaa ttcttattcc ggttgctgag tgtgatttac ttaaaccacc
    13861 aattaatatt aattgatcac gaatggcttc aaattcagcg aaggatacat gtttaccact
    13921 aaatgtattt tcagcataaa tctcatcgct aataataaat atcggatatt tttttaatac
    13981 atttacgata tttaaaactt catttctttt taaaactacg ccagttggat tagtcggata
    14041 atttaacaag acagcttttg tctttggaga aatatgactt tctaatgcat caggtgtaag
    14101 tttgaattgt gttgctgttg tatcaatata aattggttta ccacctagta cttcgatgag
    14161 tggtatgtag cctgcataaa ttggtcccgg tattataatt tcatctccag gctctatgat
    14221 agaacgtaac gttgtgtcta ttgcttcact tgctccattt gtcacaataa tttcttcagg
    14281 atcataggaa aaatgataac gatttttgaa gtattgacta attgcttcgc gagtttctaa
    14341 taacccttta ttgtgagagt atgatgtctt gtcattgtta atagcatcaa catatgcctt
    14401 tttcacaaca tcaggcatcg ggaagtctgg ttggccaata gttaaattaa cacaatcatc
    14461 caaattattc atacgatttg aaaattggcg aatacttggt gctcttaaat atttagaatt
    14521 agaatttaaa gaaagtttca tttgtgacac ctcaaatata aatcaaatat tgtctaaaaa
    14581 tttagaaatt aataatatca taacatactt ttaaagcaaa aaagggtaaa ttacttaagc
    14641 tttatttgaa aaacgaaaaa tgtctaaata cactgtagta actacttttc aaaatgaata
    14701 aagtggttac agttaatgta cttagacatt atataattat gagtctttaa actgtgatga
    14761 tctttgtaaa ttaattattg gatttgtcca tttacaaaca aagtttgtag ataatacata
    14821 tacgatgatt acagatatac ttattaaata aagatacgtc attaatgaaa taggattatc
    14881 gaatgggtac cattcaaatc ctctaacaat gccaataatt aaaccatgta ataaatatac
    14941 gtatagcgta cgactaccaa tataagtata taattttttc tttgttgaca ttaaatttag
    15001 aaatgcagtc attgcgatta atataattcc atataatata agtcgtttaa aaggactgaa
    15061 tacactctgt ccttcatttt caagtgaagt atatggtgaa cttcctaata accaatctgc
    15121 attgatagga tgaatcacgt aaatgataaa aaacaaaata aaggtaatga tagatactgg
    15181 tattagtttt ttatttttaa aaatagccgt atgttttttg gtaaaaatgt aacctaggta
    15241 aaatattggg aaaaatacga ttgtccttga aatgcttaag tagctatcga tgttatctga
    15301 aaaacctgct ccaatagata taataattga aactgatagc actttatatg gattaaatct
    15361 tctaactatt actaaaatga catgaaagaa aaatagcgtg atcaaaaacc ataacgcaaa
    15421 tactgggtta aaaggatcaa gttgtaattc gtcactttta cctgttaaga aataatatat
    15481 tgaaaagaat gcaaaaaata tcatataagg tactatcaaa cgttttgaaa ttttttctaa
    15541 atagtatggt ttatcaatat ttttcgcgaa ataaccagat ataaacaaaa atgttggcat
    15601 atggaaacta taaataacta agtataatgc tgataaatac ttatcgccac tagtgtaggg
    15661 ttgtaacata tgtccaaata cgactaataa tattaaaatt gctcttgcgt tgtcaaaaaa
    15721 ataatctctc tcttttaatg aagtcatatt ttatctcctt tatgtccata agactcttac
    15781 taatttatta tattttgtat tagttatgca acaaatttgt ctattatgtc gaatagtatt
    15841 gtaaaaatta taaaaaaagc atataatatt ataaataata tgtagtggag tagaacaaca
    15901 tgtataaaca acttgaaaaa cttattacac tgactaacaa tgacttaaac ttagtgaata
    15961 gaagatttgg acaacgcacg gatatcacat ctgaacagct agaacttctc cgtattttat
    16021 ttaattacga tcgcttatca cagtatgatt tgacgatgaa gattagcagg gaacaatcta
    16081 tagtttcaag gtggattaag aaattagttt tgaaagggta catcacaagt caacaatcta
    16141 gcgaagattt aagatgtaaa gaattaattt tgactgatca agcacgtaca ttgatttcac
    16201 aaataaataa tgcacgttgc gaattgattg aagcaagatg tcaatgttta tcggaagtcg
    16261 aattagacaa tttaaatcaa ttacttgata agttaaatca acgacgcata tcgttgtaac
    16321 acggcaatag caaacacata atttagtgtc attgtgcaca caacaaaaat tgaatcatga
    16381 attacaagca aaagtagcgg tgattgttaa aattgatggt aaacaatcac cgctattttt
    16441 gcttgtgtat gtataaaaaa gggatcaaag gtcatccccc atgattgata gtgggggatg
    16501 acttttgatc ctatgttcat gttgcttatt tatattgtgg gatgtcgaag tatttgccga
    16561 cttcgccgat tttatcatag tagcctttga tgattttagc attgatgtta gcccaatcta
    16621 catctgttgc atattggtgt gttcctggat gtgcaggatt ccatctcatt ttgtaaagcg
    16681 tattttgacc agcttttaca tatgagttgc cgatgaattt agcaccacca acgattgctt
    16741 ttgatactgt gtcccaacca gcttgtttag catatttaat accttcacgt aaaggatcgt
    16801 tatcatatgc agcaatacca aatacgttat ggtatttcgt gttagagtta gttacaactt
    16861 tgttgttcac tacatctgca ccttttgcta attgagaagt accgttacct gtttctaata
    16921 gggcatgtga gataagataa acttcattaa tgccatacat ttgagcagct ttgttaaatg
    16981 cagcaccttg attttctaat acacctttac cttttaagaa ctgattaatt ttatcaatag
    17041 aaatattttg tggttggtct aagcgtaaga attgatattt taatgctgga tcttgagcta
    17101 aacgcttcgt atccattgca tgcttaacat cattaaagtt agcatctgtc cacttacctg
    17161 gtacacgttg tacttgtggt ttatattgta aaccagcttg tatttgagca acttggttta
    17221 atgtcatacc tgtttgatta tacttaatta attctttagc taaatcagtt gatttaatcc
    17281 atgctaattt accgttagat aatttaccat agtaccaagt ttgtccatta atgacttgtt
    17341 ctttaacaac tgcgaatggt tgttcattaa atgcttttaa tgagtattta gctgtatcag
    17401 aatttggtgt tacatagtaa taaccattac catttttaat tacataagtg tagttataat
    17461 ctttggcagc tgatgtagtt ggtttaacag cagttggtgc agttaaatct tttgcattta
    17521 cccaaccagt gcggttatta atagtaccgt ataaataaac atctttgcct acagatactt
    17581 gttttgttgc attaaatgta ccttgagcaa tgttattgcc tgttaaaatg acttggtttt
    17641 tagtacccca aggaaccatt gataagccgt tatttgattt attaacagta tatttttgag
    17701 tcgttttaac ttctttgcct aagttttgaa catttaagtc ttttacattg aaccaaccta
    17761 atgggatgtt atgacttgta ttgtttaata atacatacgt ttcattacca tgagcacgct
    17821 cttttgttac atagaacgta cggtctgcat atttcgcacc gtttttcgct gttttttcat
    17881 aaacagaagc acgaatacca gtgttgtttg gtttaacttg agcaattttg ctaactgttt
    17941 gagtcgtttg tggtttagta acagcataag cttttacagc tgtttttggt tgtgctacag
    18001 cttttttagg tgcagcaggt acagctaaat atgctttact tacccaaccg gatttaccat
    18061 ttacagttcc atataaatag atagatttat caatttgttg ttgcttagtt gctttaaaag
    18121 tttggttacc tgtaccagaa accgcaccag cttcttgttt ataagtaccc caaggtactg
    18181 aatataattt agtgcctggt tttactgtat aagtttgcat tacatttaca ggtgattttg
    18241 cattgttata aataacgtca ccttgtttaa cccaaccaat taaacttgga ctattgtaat
    18301 ctttaactaa gtagaatttg tttccaccta gacttgcttc ttttgttaca gcaaatgttt
    18361 tttgaacttc tttcgttggc ttaccagttt tgtcataaac tgtagtgaat aagccattgt
    18421 ttttagcatt aatttgagca acaccattta atgatgaaac tgttaattta ttatttgttg
    18481 taggtgttga tggcttaggt gttggtgtag gtttagcagt atcaactaaa tatgctttac
    18541 ttacccaacc agatttacca ttcacagagc catataaata aattgattta tcaatttgtt
    18601 gttgctttga agccttaaat gtttggtttc cagagccaga cacgctacca gcaacttgtt
    18661 taggtgtacc ccaaggtact gtataaagtt tcgtaccagg tttgattgaa tatgattgat
    18721 ttacatttac aggtgattta gctgtgttgt aaaccacatc gccttcttta acccaaccaa
    18781 atttattacc agaattgtaa tcttggacaa gatagaattt ttgattacct aatgtagctg
    18841 ttttagatac agcaaatgtt ttttgaactt cattagttgc tttaccagtt ttgtcgtaaa
    18901 cagtagtata taagccacta tttgtcggtt taatttgagc aacaccattg tttgcagcaa
    18961 cagttaattt accagttgat ggtgttgatg gtttcgacgg tgttgttggt tttgatggtg
    19021 tagtaggggt agttgtagat tgcgtacccc atggcgccac tttacccatt tttattaagt
    19081 atttttcatt aattaagtca tatagttgat cataactata attatgactt cttaaatatc
    19141 catgtggatc ggcatggtcc gtaccaccta aatatttact tacagcgtag tgagtccata
    19201 ctgtaccatt tccatcatat tcagcactat caggtttcaa accataatat tgcaattgtg
    19261 tagctgcata gtcagcatag ttattcattg aacgtgcaaa tgaagcataa tcgtgtgtat
    19321 gaacgatttc aacattgatg aatctagggt taccgactgc accgacaccc caagataagt
    19381 aatccgttgg tgctgtttcg attatacgat ccccatcaac aaatgcatgt acgaatgcgt
    19441 tttgatagtt gtttttcata taactaattt caccatttat cgtcgaacga tcattagctg
    19501 tatcatgaac tacgatacct tcaggacgcc caacaccgtt acggtatgcg tatttaggga
    19561 agtaagatgt ataatcttct tcaatcttag gtgcttttaa gttattttta cgaatgtaat
    19621 cgttaattga agagtttact tgtggtttat attttggcaa actcgttttt ggtgttgcag
    19681 caactgatct tggttgtgct gaagcgctaa aagtagttac tttaggtgtc gcttcagttt
    19741 tagctttagg tgctgttgta gttgcagctt taggtgctgc ggctttgtat tgcgtttcaa
    19801 gagctgcagg tttagcagct gatttaatta attctggatt aatttgattt tctgaattat
    19861 catcttcatc atcaactaaa ctataaccag catttgtaac attagtgtta gttttaggtg
    19921 ctgtagtgct tgttgtcttt gcaacaggct tcgtattatt tgtagtcgct gattgattag
    19981 cacgagtgtc accatttact tgtgcagtat caactttttg acttacttga gcattgcctg
    20041 ttttgttatt tgctgttttt ggttggacaa tagcagggtc ttgatatact tgagtgccag
    20101 aaatgttttg cgttggattt tttacctcag cctttgcttg ttcagtagtt gctttaactt
    20161 tattactatc taaaacgttt ttattagtag tttgatcttg tgtcgtctca gctgcttgaa
    20221 cttgatgtgc agtgactgct gaacctacaa gcgttaatgc aaccattgat ggtagtttgt
    20281 aattgaattt tttcgccatt ctatttatta ctcctaacat ttattaatta ttactaacat
    20341 tatagtacct gttttatata cctgtgcgta tttaaccaa
//
LOCUS       DAHHRR010000038        20256 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.038, whole genome shotgun sequence.
ACCESSION   DAHHRR010000038 DAHHRR010000000
VERSION     DAHHRR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.038"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            115..909
                     /locus_tag="NBI42_001225"
     CDS             115..909
                     /locus_tag="NBI42_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1777652.1"
                     /translation="MEYIKKIAIYMSVLLLIIFIGGCGNMKDEQTNKTGSKEEQIKKS
                     FAKTLDVYPTKNLEDFYDKEGYRDGEFKKGDKGTWVIRSEMTTELKNENMESKGMVVR
                     LNRNSRTCTGEYFVRIVKEDSEGKVYSDERKYPIKIENNKIIPLKPIDDEKIKKEIEN
                     FKFFVQYGDFNGFENYKDGEVSYNPEAPIYSAQYQLKNSDYNVEQLRKRYNIPTKKAP
                     KLLLKGSGNLKGSSVGYKNIEYTFVENDEENIYFTDSINYNPSGDK"
     gene            912..2237
                     /locus_tag="NBI42_001226"
     CDS             912..2237
                     /locus_tag="NBI42_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777653.1"
                     /translation="MKKTEYTIQSDKIQSNSEETSSISQISYEIENANNHGLKKKKIY
                     NQIQSLKSDGKFPSNLEYIDSHTDKKHGVTASAFLNKDTGKVIIGMTGTNFKKEALAK
                     QLLLPDFAITKQDRIDSAETKKDLMSDAQIGTFPPSDNSKHFEETQEFIKKIKNKYDI
                     DFITGHSLGGREAVILGMSNGIPNIVVYNPAPIAARYLNHNSRLFKLYKGYKGNITRI
                     VSDKDWLTNIIKGHTNYTYFGNEIVVHNGKSHDMAGFLTEQEQKAIKKELKKVQGYVE
                     ENNKSFVINSNNAMSKLASIELLRANMMTTNGGWLSSSQQKVLESLTALTIAQSFNQL
                     IEDEINQIKKMYNEKKKKFEKNWEDAQKAGNAVGKDITVNEVLEALDEGHVNESSMVG
                     EPKQMISAKEKQLSRIGSSISNYITRVRSSINEIVDKDQALASQIGGLL"
     gene            2234..2557
                     /locus_tag="NBI42_001227"
     CDS             2234..2557
                     /locus_tag="NBI42_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777654.1"
                     /translation="MMFNQINNKNELEESYNSEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            2585..2884
                     /locus_tag="NBI42_001228"
     CDS             2585..2884
                     /locus_tag="NBI42_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777655.1"
                     /translation="MIGPVIGPDIVPDIVPDIKSMIKNAQKENIRTVIAPEHKHKYKD
                     IENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKINS"
     gene            complement(3173..3433)
                     /locus_tag="NBI42_001229"
     CDS             complement(3173..3433)
                     /locus_tag="NBI42_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777656.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYIIGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            3565..4767
                     /locus_tag="NBI42_001230"
     CDS             3565..4767
                     /locus_tag="NBI42_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD1777657.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDSEYGDRHTQFVIIGTDLDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(5426..5488)
                     /locus_tag="NBI42_001231"
     CDS             complement(5426..5488)
                     /locus_tag="NBI42_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HCD1777658.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(5552..5620)
                     /locus_tag="NBI42_001232"
     CDS             complement(5552..5620)
                     /locus_tag="NBI42_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HCD1777659.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(5672..5737)
                     /locus_tag="NBI42_001233"
     CDS             complement(5672..5737)
                     /locus_tag="NBI42_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HCD1777660.1"
                     /translation="MGIIAGIIKFIKGLIKKFTGK"
     gene            complement(5769..5834)
                     /locus_tag="NBI42_001234"
     CDS             complement(5769..5834)
                     /locus_tag="NBI42_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HCD1777661.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            6355..7839
                     /locus_tag="NBI42_001235"
     CDS             6355..7839
                     /locus_tag="NBI42_001235"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HCD1777662.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVSIYIATGEWRVPQHIAHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAF
                     SKGRMARLVGMILISIVTFIYIITHNYFYGVLQNITTHATTPPTISVIISVVILIFGS
                     LLSIWVARRRYSTAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            7852..10557
                     /locus_tag="NBI42_001236"
     CDS             7852..10557
                     /locus_tag="NBI42_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HCD1777663.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AEWLHDLKDVDIFPDKALIESAVKRGELDESIFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGMPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPKMTIESVLNHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHHFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVTKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVVGALDAIQENVSENDNANQS
                     HSVSLNATQAVDENNSELNQVGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNTIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQAGVYIPETTVFAAAEHHTSTDMLAWVYVPDT
                     LSALALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVIVQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            10719..11081
                     /locus_tag="NBI42_001237"
     CDS             10719..11081
                     /locus_tag="NBI42_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCD1777664.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(11310..11726)
                     /locus_tag="NBI42_001238"
     CDS             complement(11310..11726)
                     /locus_tag="NBI42_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777665.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVGRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     IIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            11764..12438
                     /locus_tag="NBI42_001239"
     CDS             11764..12438
                     /locus_tag="NBI42_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCD1777666.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISVA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            12475..13209
                     /locus_tag="NBI42_001240"
     CDS             12475..13209
                     /locus_tag="NBI42_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCD1777667.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSDILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDVLEENVIRFFNALT"
     gene            13618..14955
                     /locus_tag="NBI42_001241"
     CDS             13618..14955
                     /locus_tag="NBI42_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCD1777668.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIIIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            15172..16077
                     /locus_tag="NBI42_001242"
     CDS             15172..16077
                     /locus_tag="NBI42_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCD1777669.1"
                     /translation="MITYDLIGNTPLVLLEHYSEDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGADVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERSLVDGIFTIKDQDAFRNVKSLAKHEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKEIFNYEENDYE"
     gene            16070..17212
                     /locus_tag="NBI42_001243"
     CDS             16070..17212
                     /locus_tag="NBI42_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HCD1777670.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIAALENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKDTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISIGIEDTEDLVDDLKQALDTL"
     gene            17507..18532
                     /locus_tag="NBI42_001244"
     CDS             17507..18532
                     /locus_tag="NBI42_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCD1777671.1"
                     /translation="MIEFRQVSKSFHKKKQTIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSEKGMREIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGQ
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFIEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFDDTAINQYFKEKN
                     IQFEEVH"
     gene            18536..19195
                     /locus_tag="NBI42_001245"
     CDS             18536..19195
                     /locus_tag="NBI42_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1777672.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            19232..20074
                     /gene="gmpC"
                     /locus_tag="NBI42_001246"
     CDS             19232..20074
                     /gene="gmpC"
                     /locus_tag="NBI42_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HCD1777673.1"
                     /translation="MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDIKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
BASE COUNT         6929 a         2953 c         4025 g         6349 t
ORIGIN      
        1 taaattgaat aagttagttt tgtttgataa cataaaagtg taataatcac tattttatta
       61 agttgtatga aatattcaaa tgtgtataaa aataaagcag agagatgtga aatgatggag
      121 tatataaaaa aaattgctat atacatgagt gtattacttt taatcatttt tattggggga
      181 tgtggaaata tgaaagatga acaaacaaat aaaacaggtt caaaagaaga acaaatcaaa
      241 aagagctttg cgaaaacgtt agatgtatac cctacgaaaa atctagaaga cttttatgac
      301 aaagagggct atcgagatgg agaatttaaa aaaggtgata aaggtacatg ggttattaga
      361 tctgaaatga caactgaact aaaaaatgaa aatatggaat ccaaaggtat ggtcgtacgt
      421 ctaaatagaa atagcagaac atgcactggt gaatattttg ttaggatagt taaagaagac
      481 agtgagggca aggtatatag tgatgaacga aaatacccta taaaaataga aaataataaa
      541 attattccat taaaaccaat cgatgatgaa aaaattaaaa aagaaattga aaattttaaa
      601 ttctttgtac aatatgggga ttttaatgga tttgaaaatt ataaagatgg tgaagtgtct
      661 tataacccag aagcaccaat atattctgcg cagtatcaac tgaaaaacag cgattataat
      721 gttgaacaat taaggaagcg atacaatata ccgacgaaaa aagcgcctaa attattattg
      781 aaagggtcag gtaatttaaa aggctcatca gttggatata agaatattga atacaccttt
      841 gtagaaaatg atgaagaaaa tatttatttc acagacagta tcaattacaa tccgagtgga
      901 gataaataga aatgaaaaaa acagaatata ctatacagtc agataaaata caaagtaatt
      961 cagaagaaac tagttctata tctcaaatta gttatgaaat agaaaatgcg aataatcacg
     1021 gactgaagaa aaagaaaatt tacaatcaaa ttcagtcgct taaatcagat ggtaaattcc
     1081 cttctaatct tgagtatata gatagtcaca cagataaaaa acacggtgtg acagcatcag
     1141 ctttcctaaa caaagatact gggaaagtga ttattggcat gactggcaca aatttcaaaa
     1201 aggaagctct tgcgaagcaa cttttgctac cagattttgc aataacaaaa caggatagaa
     1261 ttgactcagc tgagactaaa aaggatttaa tgtccgatgc tcaaattgga acattccccc
     1321 cttctgataa cagtaaacat tttgaagaaa cacaagagtt catcaaaaaa ataaaaaata
     1381 agtatgatat tgattttatt actgggcatt cattaggtgg tagagaagcg gtaatattag
     1441 gaatgagtaa tggtattccg aatattgttg tttataatcc agctcctata gcagctaggt
     1501 atttaaatca taactcgcga ttatttaaac tatataaagg gtataaaggt aacattacta
     1561 gaattgtatc tgataaggat tggttaacta atattataaa gggacatacg aattatactt
     1621 atttcggtaa tgaaattgtg gttcataacg gcaagtcaca tgatatggct ggatttttaa
     1681 ctgaacaaga acaaaaagct ataaaaaaag aacttaaaaa agtacaaggt tatgtagaag
     1741 aaaataataa gtcgttcgta ataaattcta ataatgctat gtctaaatta gctagtatag
     1801 aattacttag agccaatatg atgactacaa atggtggttg gttatcttct tcacaacaga
     1861 aagttttaga aagtttaaca gctttaacaa ttgcgcagtc attcaatcaa ctgatagagg
     1921 acgaaattaa tcaaatcaaa aaaatgtata atgaaaagaa aaagaaattt gaaaaaaatt
     1981 gggaagacgc gcaaaaagct ggaaacgctg taggtaaaga tataaccgtg aatgaagtgc
     2041 tagaagcttt agatgaaggt catgtgaatg aaagtagtat ggtaggggaa cctaaacaaa
     2101 tgatatctgc aaaagaaaaa caactttcaa gaataggttc atctatatca aattatatta
     2161 caagagttag atctagtatt aatgaaatcg ttgataaaga tcaagcactt gcatcgcaaa
     2221 taggtggatt actatgatgt ttaatcaaat taataataaa aatgaattag aagaatcata
     2281 taattctgag aaaaaacgta tagagaatga actgcaaaat ttgaatgaac ttagacacag
     2341 aactcgaaaa gaaaatgaac gtagttatga tgtttttcaa tatttgaaac acgaaatgaa
     2401 ttatagtgaa gatgcccaaa ggaaaatgac gagaaatata gaagcgtatg agcaagaaat
     2461 caatgagata attagaaagc aagaatggaa attagaagaa tataaagaag acttaaaaaa
     2521 atcttatgaa aagcagttag ataaactaag tgattgataa tttggaggag attaaatgag
     2581 tccaatgata ggaccagtta taggaccgga tatagtaccg gatatagtac cggatataaa
     2641 gtcaatgata aagaatgctc aaaaagaaaa tattcgcaca gttattgcac ctgaacataa
     2701 gcataaatac aaagatattg aaaacggatt aaaaggtgaa gaaaaagtat tgattgaaca
     2761 aatggcgcag cattgcgaag cttttaaagc taattttaaa ggtgcagctc aaggagattg
     2821 ggttaaaagt gcaatgtctg agatagacag tattaaggat gatctgaaaa aaattaatag
     2881 ctaaactaca tatcatacta ctaaataaag cgaccaatgt tcagtgtatt cacaactgac
     2941 actaggccgc ttctttttaa tttattttat tcccatgtaa agtaattcga accgaatcga
     3001 aatatcacac tcacaattgt aaaaacacaa cttaaattac tgtcccacat taaacatatc
     3061 cacattccaa acagcaacaa caaaatcctc aagtaccaaa agctatgacc actttatcaa
     3121 aatcaacgca agttaacctc tcccgaacta cccgccataa atcatcttca ccttacaaac
     3181 cataaagcgc tgcagttaag tattgaatgg catagcctat agaaatgaaa atagcaatcg
     3241 ttataatagc actgccaatg gtatataacg tatgtttcgt agataatcct tcagtctttg
     3301 aaccaaaaac actgagtaga ataaatgcaa taccaatgat atataaaact aaaatgacaa
     3361 atagcatgtt tatacacctc gattctcttt cgtgagatat atctttgtct ccattataaa
     3421 gtaaagcgct caataattta attaagtata ctattttttt attatcgaag attgcgaaag
     3481 aggaaacact cctatataat gtaaaacgga attattccta tttgcaagcg aacgaaacaa
     3541 ataaattgaa gtgagggtat atatatggct aagattccag ttacagtatt aagtggttat
     3601 ttaggctcgg ggaaaacaac gttgttaaat catattttac aaaatcgtga aggtcgacgt
     3661 attgcggtaa ttgtaaatga tatgagtgaa gtaaatattg ataaagatct tgtcgcagat
     3721 ggtgggggac tatcgcgtac agacgaaaaa ttagtcgaac tatctaatgg ttgtatctgt
     3781 tgtacactca gagatgattt attaaaagaa gttgagcgtt tagtgaaaaa aggtggcatc
     3841 gatcaaattg ttattgagtc tacagggatt tcagaaccag tacctgttgc gcaaactttc
     3901 tcatatatcg atgatgaact tggcattgat cttacagcaa tttgccgttt agatacaatg
     3961 gttacagttg ttgatgctaa ccgcttcgtt catgacatca actcagaaga tttattgatg
     4021 gatcgtgatc aaagcgttga tgaaacagat gaacgttcga ttgcggattt attaattgac
     4081 caagttgaat tttgtgacgt attgattatt aataaaattg atttaataag tgaagaagaa
     4141 cttgcaaagt tagaaaaagt gttaagcgca ttgcaaccga ctgctaaaat cattaagaca
     4201 acaaattctg aagtagattt aaaagaagtc ttaaatacgc agcgttttga ttttgaaaaa
     4261 gcgagtgagt cagcaggatg gatcaaagaa cttgagtctg gtgggcatgc atcgcataca
     4321 cctgaaacag aagaatatgg tatatcatcg tttgtatata aacgtcgact tcctttccat
     4381 gctaagaggt tcaatgattg gttagaaagc atgccaaata atgtcgttcg atcaaaaggt
     4441 atcgtatggt tagcacaata caatcatgta gcatgtttat tatctcaagc agggtcatct
     4501 tgcaatattc atccagttac atattgggtg gctagtatgt ctgaagcgca acaaacgcaa
     4561 atattagcag aacgtcaaga tgtcgcagct gaatgggatt cagagtatgg cgatcgtcat
     4621 acacaatttg tcattattgg taccgactta gatgaagaaa aattaacaaa agaactcgat
     4681 gcatgcttag tcaatgcgca agaaattgat gctgactggc aacaatttga agatccatat
     4741 caatggcaaa ttagacctgc acgctaagtt taataaagta taatcatttt taaattgtgg
     4801 cttaattgtt tgcaatttat gacaattaat aaaaagttaa gatgttctta ggatcttttt
     4861 gttttcaatc tatctaaagt gtgatagttt tgataaagca gaaatttgca ttttatccat
     4921 agggctagga catgtatgtg tcttagtcct ttttatattt acgttgatga aaaatgacaa
     4981 aaccatcgta ccaactgtta attgaaggta caatcattgt ttaacagtca taaaaatgag
     5041 tgaagtaaat gcagctatca atgtactatt aacgcttacg gtcacacgag tgtttcagtc
     5101 taaacgtgca acgcaattcc gcacgtggcc aatatatctt gcgctagatc atgaaaatca
     5161 ttaagatttc gcacgccaaa tcatgaatgt ctcagacctc aagtatcaaa cataaaacac
     5221 gccacaaaaa tataacgcgt cagactacaa agatcacgaa tattccgtat gtcccactaa
     5281 gaacatccta tacaaaaaat accaagacca tttatttgca acaaactacc cagactcacc
     5341 tcacatcaat aaatcaacac aaagcaaagc caccatccct attggtatag tggcctgaga
     5401 attttaagta ttcaattcgc ttaaattatt ttgcgaaaat gtcgataatt gctttgatga
     5461 ttttaatgat agtacctaca atagccatcg ttttgtcctc ctgtatgttg atttaacata
     5521 gtgatgagtt gttgatcgtt aatgtttgag attagttgtt acctaaaaat ttaccaagta
     5581 aatctttaaa gaatttgaat aattttgcta cgaattccat gtgaatggcc cccttcaaat
     5641 aagatgttca tatctatgag atttttataa cttacttacc agtgaatttc ttaattaatc
     5701 ctttaatgaa tttaatgatt cctgcaatga tacccatgtg aaagacctcc tttgtttgtt
     5761 atgaaatctt atttaccagt gaattgttcg attaagcttt tgataacttt aatgatgcca
     5821 gcgatgatac ccattaagat tacctccttt gcttatgagt taacttcatt gtacatagtt
     5881 atcttgtgcg taattgattt ttttcctaac ttgaattgtt atgatcataa ctgcgaattt
     5941 tttattttaa aaggacggga aatactgcct aactgtacag gtgcatttac aaaatcttta
     6001 caatcattgg tttaatagag gttaagctca tgaatttgac atgaagcaga aatttaatta
     6061 tattttatgt tataagttta aaacgaataa cacgttaggt ctcgtctagg caaagcatat
     6121 ttatgaagat gaattgaaca gacagaagta acattttata tacatatgtg catgggagca
     6181 cgcttaaaga gaacagtgta acaaggtata tcaacatacc atggtacagt gtctagttaa
     6241 actgaaacct gcaatagtaa attttctgcc attatgtaca gaatctacta ttgtaggttt
     6301 ttatttttat acataagcaa ttatacgaga gatgaaaaat gaggaggtta tgcagtgtta
     6361 agttttcaat tgctattttc actgtttgtt attgcgctta tcattgcatt gataagtggt
     6421 ttgttgtttt tagcaccagt tatgccaatg agatatatta aattacattt atacatacta
     6481 gtcatgccag tattatttgc agtcattggc tttttcggta ttcatggtca acatgtctta
     6541 ggtccattta aaatagatcg tttatcttgg ttattagctg gctttgtaat ggcgcttggc
     6601 tttattattc aaaagttttc aatgcgatat ttactaggtg atcatcatta tagacattac
     6661 tttccattgt tcactgcgat tacgtcgttt gcatctttag catggatgtc tgaagactta
     6721 agactgatgg cactctgctg gggtatgaca ttattatgtt taacattgct gatgaacgtt
     6781 aatcgttttt ggaaagtgcc acgtgagtct gcgaaattat caagcatgac atttttatgt
     6841 ggttggcttg cattcgttgg agcaattgta tctatttata ttgcaactgg cgagtggcgg
     6901 gtaccacaac atatagcgca tccgacatgg tcattgttga cgaatgtact acttgtatta
     6961 gctgtcatga taccggcagc acaatttcct tttcatcgat ggttgattga atctgtaact
     7021 gcaccaacgc cagtatcggc aattatgcat gcaggaattg tgaatgcagg tggtgttatt
     7081 ctaactcgtt ttgcgccgat atttgataat ggatttgcgt tatcattatt acttatcctt
     7141 tctagtattt cagtattgtt aggatcaggc ataagtttgg ttcaagttga ctataaacgc
     7201 caattagtgg gctctacgat gagtcaaatg ggctttatgt tagtgcaatg cgcacttggt
     7261 gtatattcag cagcgataat acatttaata ttgcacggta tttttaaagc aacattattt
     7321 ttacaatcag gttctatcgt gaagcgattc aatattccaa aacaagcatc tgctaaagac
     7381 gcttatggct ggattgtcat gggacgtgta ttagctatta tcgtggcatt tctgttttgg
     7441 atgagtagtg acagaagtgc atatgaagtg ttaagtgcac taattctagc atggtcatta
     7501 cttgtatctt ggaatcaaat ggtagccttt agtaaagggc gtatggcacg tctggttggc
     7561 atgattttga tttcaattgt gacatttatc tacatcatca cacataatta tttttacggt
     7621 gtattacaaa atataacaac acatgcgaca acaccgccta caattagtgt catcattagt
     7681 gttgtcatat taatatttgg tagtttatta agtatctggg tagcgcgtcg tcgatattct
     7741 acggcttttg cagtgttata tgtgtggtta gtaaatttag gtgaagcacg ttcgaaagcg
     7801 atagaaagtc atccgaatta tttgaagaag tatttatagg aggtgaaagg tatgacaaca
     7861 cagttaaata tcaattcagt cattgaaaat gcgaaacgtg ttattacgcc gttatcaccg
     7921 atttcaattt ttgctgcacg taatccctgg gaaggattag aagcagatac atttgaagat
     7981 gttgccgaat ggttacatga ccttaaagat gtagatattt ttcctgataa agcgttaatt
     8041 gaaagtgctg taaaacgtgg tgaactagat gaaagtatct ttaatcaact agtaactgat
     8101 atgttgcttg aacatcacta caatatcccg caacactaca tcaatcttta tattgataac
     8161 attaaaacat taaaagacgt acctgcatca tatatgaatc attcaaatgt tgatgttgtt
     8221 gctgatctac tattagaaaa atcaaaacgt gatatggctg aatcatatca tcactatgat
     8281 gtacgtccga tgagtgatgc aataatagat gaacaaggta tgccacttag cgaacaagtg
     8341 aatcgtcaaa tgattaaatg gacgaaactt tatatcgacc aatttttatc gagttggaca
     8401 atgccgaagc gtgaacaaag tttttaccat gcatggttgc atttagcgca acatgaccat
     8461 agttttacta aagcacagcg ccaagtgatt aaaggattac ccaatgatcc taaaatgacg
     8521 atagagtcag tattaaatca tttttcaata gatcaggaag actaccaagc ttatgttgaa
     8581 ggacatcttt tggcgttacc gggttgggca ggtatgttgt attaccgttc acaacagcat
     8641 cactttgaac aacatttgtt aacggattat ttggcaattc ggttagttgt cgaacaattg
     8701 ctagttggtg atgagtttaa gtcagtcact aaagattgtg aaagtagatc ggaaaattgg
     8761 tttaagcaaa ctgttgcatc atggtgttac tacagtgata tgcctagcga tgtattacta
     8821 caacatgacg tcaatgaaat tcaaacgttt attcattttg cagcaactat gaataaaaat
     8881 gtatttaaaa atttatggct aattgcctgg gaaatgacat acgaatctca gttaaaacaa
     8941 aaaattaaag caggtcatga aagtgtggtg ggcgcattag atgcaattca agaaaatgtt
     9001 tcggaaaatg acaacgctaa tcagtcacat tcagtatcgt taaatgccac acaagcagtt
     9061 gatgaaaata atagcgagct aaatcaggtg ggcacatcaa cgaaagcgca aattgcattt
     9121 tgtatagatg ttcgttcaga accatttcgt agacatatcg aagcagcagg accctttgaa
     9181 acgattggta ttgcaggctt ctttggatta cctattcaaa aagatgccgt agacgaacaa
     9241 ttcaaacatg attcattacc tgtcatggta ccgccggcat atcgcattaa agagtttgca
     9301 gaccgttacg atatgaatgt ttatcgacaa cagcaacaga caatgtcatc gatgttttac
     9361 acatttaaat tgatgaaaaa taatgtcatg cctagtctgt tattgcctga attaagtgga
     9421 ccatttttaa gcttgagtac cattgtcaat acgattatgc ctagaaaaag tcgcgcgtct
     9481 ttacagaaaa taaaacaaaa atggttgaag aagcctgaaa caaagttaac gattgatcgg
     9541 gagtttgaca gaacatcaga cttacctgtt ggatttactg agcaagagca aattgatttc
     9601 gcgttacaag cgttgaaatt gatggattta accgaagcat ttgcgccgtt cgttgtgtta
     9661 gcaggtcatg ctagtcattc tcacaataat ccacatcatg catcacttga atgtggggct
     9721 tgtggtggcg catcaagcgg ttttaatgct aagttattag cgatgatatg caatcgtcca
     9781 aatgtcagac aaggattaaa acaagcaggc gtgtatattc cagagacaac tgtttttgcg
     9841 gcagcagaac atcatacgtc tactgatatg ttggcatggg tatatgtgcc agacacatta
     9901 tcagctttag ctctagatgc atatgaatca ttgaatgacg ccatgccgat gatttctgaa
     9961 cacgcgaatc gcgaacgttt ggacaaactg ccaactattg gtcgtgtgaa tcatccagtg
    10021 gaagaagcgc agcggtttgc aagtgattgg agtgaagtac gtccagaatg gggcttggct
    10081 aaaaatgcat catttataat tggacgacgc caattaacaa aaggcattga tttagaaggg
    10141 cggacatttt tacacaatta tgattggcgt aaagataaag atggcacatt attaaatacc
    10201 atcatttctg gtccggcact tgtggcacaa tggattaatt tacaatatta tgcgtcgaca
    10261 gttgcgccgc atttttacgg aagtgggaat aaagcgacac aaaccgtcac gtcaggtgtt
    10321 ggtgtcatgc aaggtaatgc gagtgatctg atgtatggct tatcatggca atctgttatg
    10381 gctgctgatc gaacgatgta tcattcgcca attcgtttgc ttgtcattgt tcaggcaccc
    10441 gactatgttg tagcaagact actcgcgaat aatgagcatt tcgctaggaa ggtgtctaat
    10501 cattggctgc gtttaatgag cgttaatgag gaagggcgtt ttaaaagttg gatttaacga
    10561 tgaaatgttg cgatgctaag taactaatat atcatcatat ctacaatgtt ctagcttctt
    10621 aaaatatact aaaaatgata aacttaatgt aatagttgat gtcgtgataa cgatacaaag
    10681 atgaagtaat caattttaat attattggag ttagtgatat gaaaagaacg aaaggtgaaa
    10741 tcgaagctga aatcagtaaa gccattacgc aatgggaaaa agatttcctt ggtagaggtt
    10801 ccttgtcggt taaatcagac attttaagag atatggtgat tattagttta caaggtatct
    10861 taacgccagc agaatatcgt gtgtgtagta cgaatgaagg attattaaat attaaacgaa
    10921 cacgttctga attagttgaa tccggtgagc aagatttgaa tgatatcatt tttaaaatta
    10981 caggtatcaa agtgatgagc ttccatagtg atttaagtac agttacaggt gaacgtatta
    11041 tcgtattcaa acttgaggat aatttggaaa agcatattta agaaggttag gtatgcttct
    11101 gctggcttat taaagctata gaagtatgct tggttaaaca gcgcaacgcc atatatttgg
    11161 gtgtgctgtt tttctattat atgatgtgtt tattacgtgg atgaatgcgt gggtctgaat
    11221 atagcctgtg aaaggcgtat gtcataaaat ttgcgaatgg tgacaagtca acgcaagcat
    11281 atttgttgcg agacatgccc actatccttt tacataaaga gtatatgtgt gacgtatgca
    11341 tataatcgat aaagtatacc taaaaaatta ggtataatga ccattgttgc aataagtttt
    11401 tcggccttga aaccgataat gcatagtata ataggaataa aatataatac aaatatccag
    11461 taaatcaaat ttgaaaacga tggatgtata ggggtaaaga atctaactaa tgtaatgatt
    11521 aacgctatat agacaaaaat tactctattg atacgcccta ccccctctgt ataataaata
    11581 tagatcgtac taaattgaaa atagcaaaat attgttattt caattataca atgttttatt
    11641 tgcaatatac ataacaatat gaactttata attttgatat tgtcggtaaa gtgaaatttg
    11701 tggcacattg atattatgga taacatttgt aaaatgaaaa tgatgatacg aggagattgt
    11761 aagatgatag ataaaaaatt aacatcaccg aaaatgacag tgcctttatt tttaatcgcg
    11821 ctgattgtat ttataggtat gttttacagt gtagtgacaa atcaagaatg gcttaaaaat
    11881 atagatatgg gatcattaac gtggtttaca gattatttcg gtgagccaca acgtcagtat
    11941 gttaacaatt tgtttaatta ctatatgacg tttagtgcgg aaattggaga tgtcaaaggt
    12001 gtcgtgttga tttccattat cgtcacaatc atactgttta ttaaacagag gcatttagcg
    12061 gtttggtttg tgacatattt ggtttcaggt gtcatcatga acaaattaat taaagatact
    12121 gtattacgtc caagaccata taatcattta gccgttgata caggcttttc atttccgagt
    12181 ggacattcca acgccagcac attattatat ttcgccttaa tgatcattat tatttcagtt
    12241 gctgctaaga caataacaaa agtgttgagt gcgctagtta tgggaatatt atggcttagc
    12301 atattatttt gtcgccttta ttttcatgca cattactttt cagatgtcat tggcggcacg
    12361 tcactagcaa tcatttgggt agcgttattc ttaatggtat acccatactt tattaatcat
    12421 cgacgacaac gcgtttagca aacatcttta gacatagtgg aggtatatag aaaaatgaga
    12481 attaaaacac cgagtccatc ttatttaaaa ggcacaaatg gacatgcgat attattatta
    12541 cattcattta caggtacaaa tcgggatgtg aagcatcttg cagctgagtt aaatgatcaa
    12601 ggatttagtt gttatgcacc gaattatcca ggtcatggct tactgttgaa agatttcatg
    12661 acatataatg tagatgattg gtgggaagaa gttgagaaag cttaccaatt tttagtcaat
    12721 gaaggttatg aatctatcag tgcaacgggt gtgtctttag gtggattaat gacattaaaa
    12781 ttggcgcaac actatccttt gaaacgtatc gctgtcatgt cagcaccaaa ggaaaagagt
    12841 gacgatggtt taatagaaca tttagtttat tatagtcaac gcatgtcgga tattttaaat
    12901 ttagatcagc aagcatcgag tgcgcaatta gcagcaattg atgattatga aggtgaaatt
    12961 acgaagtttc aacattttat tgatgatatc atgacaaatt taaatgttat taaaatgcca
    13021 gctaatatat tatttggtgg taaagatgcg ccatcctatg aaacaagtgc acactttatt
    13081 tatgaacatt taggatcagt agacaaagaa ttaaatggtc tgaaggattc gcatcattta
    13141 atgacgcatg gagaaggcag agatgtttta gaagaaaatg ttattcgctt tttcaatgct
    13201 ttaacataat tgaagatttt aaaaattaac acttcaatga cgatgttatt atatttgtca
    13261 tttgagtgtt ttttgtggcg tgatagtata ggagacttag ctaaagttga tttgtattgt
    13321 aatgtcgtgg gaatgttatg ggcattgtac ctgaagttgt tatgtggcga gatgttgggc
    13381 attgtacctg aagttgttat gtggtgagat gttggcgcac gtatacagca ttgaaccaga
    13441 aattttaatg tagcgtaatg acaacacatt acaagagttg attatattat gataaatcca
    13501 ggcgctaccg tattaatgaa acaattttga tgcaggtgta ttgcatgtta tttacataca
    13561 tgttagagtg ttacttaaac acgtagacgc tttaacgctt taaaggagat gtgaatgatg
    13621 aaaagacaac aatcacaatg gaagtcatca actggattta ttttagctag tgcgggttct
    13681 gcaatcggtc ttggtgccat gtggaaattc ccatatatgg cagggattta tggcggcggt
    13741 gcctttctag ctatgttctt aatattcacc atttttgttg ggttaccatt actcattatg
    13801 gaattcactg ttgggaaaat gggacggaca tatacaacac aaatatatag taaattaact
    13861 ggtaaaaaat ggctcaatat cattggctgg aacggtaatt tggcagtgtt tattttattt
    13921 ggcttctata gtgttatcgg tggttggatt gtcatttaca tcggacaagt attatggcaa
    13981 ttagttatat ttcaacgcat caatcatctc caagaaatga attttgaagc ggtaatatca
    14041 aatccttggt taaccgttct agggcaaggt atattcatat tcgctacgat gattattgtc
    14101 atgttaggtg ttgaaaaagg attagaaaag gcatcgaaag ttatgatgcc attgctgttt
    14161 gtctttttaa tcatcattgt gattaagtct ttaacattag atggtgcctt agaaggtgtg
    14221 aaatttattt tacaaccaag agtatcagag attactgctg atggaatctt gtttgcgcta
    14281 ggccaatcat tctttacgtt atcattagga actacaggta tgattactta tgcgagttat
    14341 gcctctaaag acatgacgat taagtcatca gctatttcta tcgttgttat gaatatcttt
    14401 gtatctgtat tggcaggcct agctatattt ccggcgttac atagttttgg ctatgaacca
    14461 caagaagggc ctggattatt atttaaagta ctgccaatgg tctttagtca aatgcatcta
    14521 ggcacattat tctatttggg attcttagtg ctgttcttat ttgcggcttt aacgtcatct
    14581 atttctttat tagaattaaa tgtttctaac ttcacgaaga atgacaatac aaaacgtaaa
    14641 aaagtcgcag tgatcggtag tattttagta tttatcatta gtattccagc aaccttatct
    14701 tttggtatct taaaagatgt aagattcggt gcgggaacga tttttgataa tatggatttc
    14761 atcgtttcga atgtattgat gccattaggc gcattaggta ctacgcttgt cgtaggacaa
    14821 ttattagata aaaaattatt acaacaatat tttggtaaag atcgatttag attattcagt
    14881 ggttggtatt acttaattaa gtatgcgatg cctgtcgtta ttattttagt ctttatcgtg
    14941 caattattta gttaatatat agaagcatct gacacacaag tatttgtgtt gctcaggtgt
    15001 ttttttgttt ggaattgaaa agttagtact ggttctgaat taatcgaaac ttagataatt
    15061 gcaaaataac aaaatgtcgt ttataattct tatctgaaaa gtaagaattt aaattgagac
    15121 atgaagaaag gtttcattca tttgatcggc atgtcagatg agggtgcatc tatgattact
    15181 tatgatttaa ttggcaatac accattagta ctgttagaac attatagtga ggataaagtt
    15241 aaaatttatg ccaagcttga acaatggaat cctggaggca gtgttaaaga cagactcggg
    15301 aaatatttag tagagaaggc aattcaagaa gggcgtgtgc gtgcaggtca aactattgtt
    15361 gaagcgactg ctggcaatac aggcataggg ttagctattg cagcaaatag acatcatttg
    15421 aaatgtaaga tctttgcgcc gtatggtttt tcagaagaaa agattaatat tatgatagcg
    15481 cttggtgcag atgtttcaag aacgagtcag tctgaaggta tgcatggggc acaattagct
    15541 gcacgttcct atgctgaaaa atatggtgcc gtttatatga atcaatttga atccgaacat
    15601 aatccggata catattttca tacattggga cccgaattga cttcagcatt acagcaaatt
    15661 gattattttg tggctggtat tggctctggc ggtacattta caggtaccgc acgttattta
    15721 aagcaacatc acgtgcaatg ttatgccgtt gagccagaag ggtccgtgtt aaatggaggg
    15781 ccagctcatg cacatgacac tgaaggtatc ggttctgaga aatggccgat atttttagag
    15841 agaagtcttg tagatgggat atttacgatt aaagatcaag atgcctttcg aaatgtcaaa
    15901 agtttggcaa agcatgaagg attactggta ggcagttctt caggtgcagc attacaaggt
    15961 gcattgaatt taaaagcgca actatctgaa ggtacgattg ttgtcgtatt tccagatggt
    16021 agtgatcgat atatgtctaa ggaaatattt aattatgagg agaatgatta tgaataagaa
    16081 aactaaatta attcacggtg gacacacaac ggacgactat acaggtgcag ttacaacacc
    16141 aatttatcaa acaagtacat atttacaaga tgatattggt gatttacgtc aaggatatga
    16201 atattctcgt actgcgaatc caacaagaag ttctgtagaa agcgttattg cggcattaga
    16261 aaatggcaaa catggctttg catttagttc aggtgttgca gcaatcagtg cagttgttat
    16321 gctgttggac aaaggagatc atattatttt aaattcagat gtatacggcg gtacttatcg
    16381 cgcattgaca aaagtattta cacgatttgg cattgaagtg gattttgtag atacaacgca
    16441 tacagattca attgtacaag cgatacgccc aacaacaaaa atgttgttta ttgaaacacc
    16501 ttctaatcca ttattacgtg ttactgacat taaaaagtcg gctgaaattg cgaaagaaca
    16561 cggtttgatt tcagtcgttg ataacacatt tatgacacct tattatcaga atccattaga
    16621 tttaggcatc gatattgtct tacattctgc aacgaaatat ttaggtggac atagtgatgt
    16681 cgttgctggt ttagttgcaa catcggatga caagcttgca gaacgtttag catttatttc
    16741 aaattcaaca ggtggcattt taggacctca agatagctat ttacttgtta ggggtattaa
    16801 aacattaggt ttacgtatgg aacaaattaa tcgcagtgtt attgaaatta ttaaaatgtt
    16861 acaagcacat ccagctgtgc aacaagtatt ccatccaagt attgaaagtc atttaaatca
    16921 tgatgtccat atggctcaag cggatggcca tacaggtgtg attgcatttg aagtgaaaga
    16981 tacagaaagt gccaaacaat tgattaaagc aacatcgtat tacacattgg ctgaaagttt
    17041 aggtgcagtg gaaagtttaa tttcagtacc tgcactgatg acacatgcat caattccagc
    17101 agatattcga gctaaagaag gtattacaga cggacttgta agaatttcta taggtattga
    17161 agatactgaa gatttagtag atgatttaaa acaagcacta gatacgttat aaataatagc
    17221 agcactggca tatattttga gttcaacgtt tgttgttctt gaatatgcta gtgctttttt
    17281 agtgtgatgt taatatttcg taattgcgtt gtggaggatt gctggtcatt tttatggatt
    17341 taattcgata aatgtcatag tagtctcaca attcatcatt gtcacatgaa tcgcttattt
    17401 tttaattttt tagaaaattc ggcaaattgt attgacgata tatgcaattt tacatataat
    17461 gctcttattc ctagtggatt aataagaatt tgtaggaggg gcgatgatga ttgagtttcg
    17521 acaagttagt aaatcatttc ataagaaaaa gcaaacaata gatgctttga aggacgtatc
    17581 atttacggtc aatcgcaatg atatttttgg tgtgattgga tatagtggtg caggaaaaag
    17641 tacgttggta agactcgtga atcatcttga agctgcctcg aatggacaag tgattgtaga
    17701 tggacatgat attacgaatt atagtgaaaa aggcatgcga gaaataaaaa aagatatcgg
    17761 tatgatattt cagcatttca atttattaaa ttcagctacg gtatttaaaa atgtagctat
    17821 gccgctcatt ttaagtaaaa aaagcaaaac agaaattaag caacgagtaa cggaaatgct
    17881 tgaatttgta ggattgagtg ataaaaaaga ccaatttcct gatgaattat ctggtgggca
    17941 gaagcaaagg gtggctattg caagagcgct tgttactaat ccgaaaatac tcctatgcga
    18001 tgaagcaaca agcgcattgg atccagcaac gactgcttcg atattgacgt tattaaagaa
    18061 tgtcaatcaa acctttggca ttacaattat gatgattaca catgaaatgc gcgttattaa
    18121 agacatttgt aatcgtgttg ctgtaatgga aaaggggcaa gtggttgaaa cagggactgt
    18181 taaagaggta tttagtcacc ctaaaacgac gattgctcaa aattttgtgt ctacagttat
    18241 acagactgag ccaagtacgt cattgattcg gcgattgaat gacgaacaag ttggcgattt
    18301 taaagattat aaaatcttca tcgaggaaac tcaggtgaca caaccgatta taaatgactt
    18361 gattcaaatt tgtggtagag aggttaaaat tttattttca tctatgtcag aaatacaagg
    18421 taacaccgta tgttatatgt ggcttcgatt taatatggat caacaatttg atgacacggc
    18481 tataaatcaa tatttcaaag agaaaaatat tcaatttgag gaggtgcatt aacatatgtt
    18541 tggttctgat ttagacagtg cacagttatt acaagcatta tacgaaacgc tatatatggt
    18601 atctattgct ttgtttttag gagcagtgat tggtattcca ttaggtgtct tattggtaat
    18661 tactcgaaaa caaggcatat ggcccaatat agtgatacat caagttttaa atcctttaat
    18721 caatatttta aggtcactac catttattat tttgttaatt gcgattgtgc cattcacaaa
    18781 attagtagta ggtacttcaa ttggtacgac tgctgccatc gtgcctttaa cagtatatgt
    18841 ggcaccttac attgcaagac ttgttgaaaa ctcattattg gaagtagacg aggggattat
    18901 tgaagcggcg aaagcgatgg gcgcttcacc actacaaatc attagatatt ttttaattcc
    18961 tgaagcgtta ggttcattag tattagcaat taccactgcg attattggac ttattggaag
    19021 tacggcgatg gcaggagctg ttggcggtgg tggtatagga gacttagctt tagtgtatgg
    19081 ttatcaaaga tttgatacga cggtcattat tattaccgtt attgtattag tcattattgt
    19141 ccaagtgatt caaacgctag ggaatgtcct agctaggttc atacgtagac attaatgata
    19201 tatagtgaag attttgaaag gaattgatag aatgaaaaga ttgattgggt tagttatcgt
    19261 agcacttgta ttattagcag cgtgtggtag taacaatgat aaaaaagtaa caattggagt
    19321 cgcatcaaat gacactaagg cttgggagaa ggttaaagaa ttagctaaaa aagatgatat
    19381 tgacgtggag attaagcact tctccgatta caatttaccg aataaagcat taaacgatgg
    19441 tgatattgat atgaatgcat tccaacattt tgcattttta gatcaatata agaaggcgca
    19501 taaaggaaca aagatttcag cattgagtac aacagttttg gcaccgttgg gcatttattc
    19561 ggataaaatt aaagatatca aaaaggttaa agatggtgct aaagttgtca ttccaaatga
    19621 tgtatcaaac caagcacgtg cacttaaact attagaagca gctggtttaa taaaactgaa
    19681 aaaagatttc ggtttggcag gcacggtgaa agatataacg tcaaatccaa aacatttaaa
    19741 aattactgca gtagatgcac aacaaactgc acgtgcttta tctgatgtcg atattgcagt
    19801 tattaataac ggtgtagcaa ctaaagcggg gaaagaccct aaaaatgatc cgatattttt
    19861 agaaaaatca aattcagatg ctgtaaagcc atatattaat attgttgcag ttaatgacaa
    19921 agacttggat aacaaaacat atgctaaaat cgtagaattg tatcattcaa aagaagctca
    19981 aaaagcgttg caggaagatg tcaaagatgg agagaaacct gttaatttat ctaaagatga
    20041 gattaaggca atagaaacgt cattagcaaa ataaattata ttgcgtccta caagcaaagt
    20101 tcatgcttat gtttgtaggg cgttattgtt tgagaataaa attatttcca atagagaaag
    20161 ggattgtaat cattttatag tgaaatatta tgaaattgta ataatttaga tattgtaaaa
    20221 tctaataagt tgtaataatt ttaaggggta attata
//
LOCUS       DAHHRR010000039        19607 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.039, whole genome shotgun sequence.
ACCESSION   DAHHRR010000039 DAHHRR010000000
VERSION     DAHHRR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19607
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.039"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            70..1599
                     /gene="aur"
                     /locus_tag="NBI42_001247"
     CDS             70..1599
                     /gene="aur"
                     /locus_tag="NBI42_001247"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HCD1777674.1"
                     /translation="MRKFSRYAFTSMAAVTLLSTLSPAALAIDSNNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKHKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            1966..2415
                     /locus_tag="NBI42_001248"
     CDS             1966..2415
                     /locus_tag="NBI42_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCD1777675.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2418..2585
                     /locus_tag="NBI42_001249"
                     /pseudo
     CDS             2418..2585
                     /locus_tag="NBI42_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2762..3997
                     /gene="arcA"
                     /locus_tag="NBI42_001250"
     CDS             2762..3997
                     /gene="arcA"
                     /locus_tag="NBI42_001250"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCD1777676.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLRDEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            4030..5040
                     /gene="argF"
                     /locus_tag="NBI42_001251"
     CDS             4030..5040
                     /gene="argF"
                     /locus_tag="NBI42_001251"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCD1777677.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQFGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5131..6561
                     /gene="arcD"
                     /locus_tag="NBI42_001252"
     CDS             5131..6561
                     /gene="arcD"
                     /locus_tag="NBI42_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCD1777678.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSIIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFGMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASLYAIWLIYAAGINYLLLTMLLYIPALLVYSIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6578..7519
                     /gene="arcC"
                     /locus_tag="NBI42_001253"
     CDS             6578..7519
                     /gene="arcC"
                     /locus_tag="NBI42_001253"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD1777679.1"
                     /translation="MKEKIVIALGGNAIQTKEATAEAQQTAIRRAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKNDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7618..8322
                     /locus_tag="NBI42_001254"
     CDS             7618..8322
                     /locus_tag="NBI42_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCD1777680.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLLCHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            8672..11449
                     /gene="clfB"
                     /locus_tag="NBI42_001255"
     CDS             8672..11449
                     /gene="clfB"
                     /locus_tag="NBI42_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HCD1777681.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASVDSEKNNTIETPQLNTTANDTSDISANTNSANVDSTAKPMST
                     QTSNTTTTEPASTNETPQPTAIKDQATAAKMQDRTVPQEANSQVDNKTTNDANSITTN
                     SELKNPQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNGQ
                     VTASDFKLEKTTFDPNQSGNTFMSANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNDKENINGQFSLPLFTDRAKA
                     PKSGIYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADP
                     NDSNLKEVTDQFKDKITYKYQNVASINFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     IDPATGKDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPNPEPEPSPEP
                     EPEPTPDPEPEPEPTPDPEPSPDPEPEPTPDPEPSPEPDPDSDSDSDSGSDSDSGSDS
                     DSDSDSNSDSDSESDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSNSDSDSDSDSDS
                     DSDSDSDSDSDSDLDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSNSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDLDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTP
                     PNNEQKAPSNPKDEVNHSNKASKQHKTDALPETGDKSENTNATLFGAMMALLGSLLLF
                     RKRKQDHKEKA"
     gene            11565..12326
                     /locus_tag="NBI42_001256"
     CDS             11565..12326
                     /locus_tag="NBI42_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCD1777682.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNNDTTAKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDHL
                     SRKNVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            12571..13026
                     /locus_tag="NBI42_001257"
     CDS             12571..13026
                     /locus_tag="NBI42_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD1777683.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(13129..13239)
                     /locus_tag="NBI42_001258"
                     /pseudo
     CDS             complement(13129..13239)
                     /locus_tag="NBI42_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13309..14733)
                     /locus_tag="NBI42_001259"
     CDS             complement(13309..14733)
                     /locus_tag="NBI42_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCD1777684.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALNKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            14931..15131
                     /locus_tag="NBI42_001260"
     CDS             14931..15131
                     /locus_tag="NBI42_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777685.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQAKEQKDDKV"
     gene            15157..15822
                     /gene="nsaR"
                     /locus_tag="NBI42_001261"
     CDS             15157..15822
                     /gene="nsaR"
                     /locus_tag="NBI42_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HCD1777686.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQHQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSMTVKGCTLILDEAKVVYQEQSIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            15833..16720
                     /gene="nsaS"
                     /locus_tag="NBI42_001262"
     CDS             15833..16720
                     /gene="nsaS"
                     /locus_tag="NBI42_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCD1777687.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLYVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            16828..17583
                     /locus_tag="NBI42_001263"
     CDS             16828..17583
                     /locus_tag="NBI42_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1777688.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLTVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            17580..19580
                     /locus_tag="NBI42_001264"
     CDS             17580..19580
                     /locus_tag="NBI42_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1777689.1"
                     /translation="MKLRLLWHIMRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTIMKLIANDYDLSFNDNSKSQ
                     IERQQMIENIKRDIQGSVNVKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKKSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVIAVIIISFLYMAMYKITSYIYAKVIHQQRN"
BASE COUNT         7007 a         2944 c         3636 g         6020 t
ORIGIN      
        1 tacttttctg aaaaattaaa ttaatatttt atttataagc tgtttttaag atttcaggag
       61 gaatgaaatg tgaggaaatt ttcaagatat gcatttacaa gtatggcagc agtaacattg
      121 ttgagcactt tatcaccagc agcattagcg attgattcaa acaataaacc agctaattct
      181 gatattaaat ttgaggtgac tcaaaagagt gatgcggtca aagcattaaa agaattgcct
      241 aaatccgaaa atgtaaaaaa tatttatcaa gattacgctg ttactgatgt aaaaactgat
      301 aaaaagggat ttacgcatta cacattgcaa ccgagtgttg atggtgttca tgcacctgac
      361 aaagaagtga aagtacacgc agacaaatca gggaaagtcg ttttaatcaa tggggatact
      421 gatgcgaaga aagtaaagcc aacgaataaa gtgacattaa gtaaagatga cgcagccgac
      481 aaagcattta aagcagttaa gattgataag cataaagcga aaaatcttaa agataaagtc
      541 attaaagaaa acaaagttga aatcgatggt gacagtaata aatacgttta taatgttgag
      601 ttaattacag tgacaccaga aatttcacat tggaaagtta aaattgatgc tcaaactggc
      661 gaaattttag aaaaaatgaa cttagttaaa gaagctgcag aaactggtaa aggaaaaggt
      721 gtacttggcg atacaaaaga tatcaatatc aatagtattg acggtggatt tagcctagaa
      781 gatttaacgc atcaaggtaa attatcagca tttagcttta atgatcaaac aggtcaagca
      841 acattgatta ctaatgaaga tgaaaacttc gtaaaagatg agcaacgtgc tggcgtagat
      901 gcaaattatt acgctaaaca aacatatgat tattacaaag acacatttgg tcgtgaatca
      961 tatgacaacc aaggtagtcc aattgtttca ttaacgcatg ttaataacta cggtggtcaa
     1021 gataacagaa ataatgccgc atggattggt gacaaaatga tctatggtga tggtgatggt
     1081 cgcacattca caagtttatc gggtgcaaat gacgtagtgg cacacgaatt aacacacggt
     1141 gtgacacaag agactgcgaa cttagaatat aaggaccagt caggcgctct aaatgaaagc
     1201 ttttcagatg tttttggata ctttgtagat gacgaggatt tcttaatggg tgaagatgtc
     1261 tacacacctg gaaaagaggg agatgcttta cgtagcatgt caaacccaga acaatttggt
     1321 caaccagctc atatgaaaga ctatgtattc actgaaaaag ataatggtgg cgtacatacg
     1381 aattctggaa ttccaaataa agcagcttat aacgtgattc aagcaatagg gaaatctaaa
     1441 tcagaacaaa tttactaccg agcattaacg gaatacttaa caagtaattc aaacttcaaa
     1501 gattgtaaag atgcattata ccaagcggct aaagatttat atgacgagca aacagctgaa
     1561 caggtgtatg aagcatggaa tgaagtaggc gtggagtaaa aatatataaa caagaagaag
     1621 taatgttaaa cacttataaa taattaaatt ttaaatacag cttatatatg gggtatacct
     1681 acattgataa ttgactttgt aggtataccc tctctttgta attagatgag aggagtgtga
     1741 gggttgtctg ccgaaagact actcggcagt ttaaaatcat tacaagtagt agatatgtga
     1801 taattaaatg ctgacttaga atacaaaatt cattttaaaa gttgtcacaa aaaatttaca
     1861 tgtattttta ttatcttttg caaaacaaag tgttaaatta taaatgaaac atgcatgaat
     1921 ttatttttta atacaagaaa cgtaactacc aaaggagttt acaatatgaa gaaaagtaaa
     1981 cgattagaaa ttgtttctac aatagttaaa aagcataaga tttataaaaa agaacaaatc
     2041 atttcatata ttgaagaata ttttggtgta agatatagtg caacaacaat tgctaaagac
     2101 ttgaaagaac ttaatatata tcgtgtccct atcgattgtg aaacatggat ttataaagct
     2161 attaataatc aaacagaaca agagatgaga gaaaagttta gacactattg tgaacatgaa
     2221 gttctaagtt caatcatcaa tggttcatac attatcgtca aaacctcacc tggtttcgcc
     2281 caaggcataa actattttat cgatcagcta aatatagaag agatattagg tacggtgagt
     2341 ggaaatgaca ctacattaat cttaactgcc tcaaatgata tggcagaata tgtatatgca
     2401 aaattattta aatagacatg tatcaaatga ataataaaaa ttagtttcgt ataacgtgta
     2461 ctcaagttag ttaccatata ttaacttgtg tacgcgtttt tttatggaaa gaaagaattc
     2521 atagtcattc atttgactgt ataaaaaact ttatacaaca tgtttttgca ggtctttttg
     2581 aataaaaaat gtatatttag acccaaaata cctttatcta tgtataaaaa tccattatta
     2641 tgtattgtat aacaaaaaga tatgaaattt tcgactttct ttatgtgaat ataatcacat
     2701 gtaagcgttt gaagattgtc tatactctaa atgaattcaa agataaaagg aggaaataga
     2761 catgacagat ggtccaatta aagtaaatag cgaaattgga gctttaaaaa ctgtgttgct
     2821 taagcgtcct ggaaaagaat tagaaaattt agtacctgat tatttagatg gattactatt
     2881 tgatgatatt ccatatttag aagtagctca aaaagagcat gaccattttg cgcaggtgct
     2941 aagagacgag ggtgttgaag tactttacct tgagaagtta gcagctgaaa gtattgaaaa
     3001 tcctcaagta agaagtgaat ttattgatga tgtattagca gagtctaaaa aaacaatatt
     3061 aggtcatgaa gaagaaatta aggcattatt tgcgacactt tctaatcaag aacttgtaga
     3121 taaaataatg tctggggtac gtaaggaaga aattaatccg aaatgtacac atctagtaga
     3181 gtatatggat gataagtatc cattctattt agatccaatg ccaaaccttt attttactag
     3241 agatccacaa gcctcaatag gacacggtat aacaatcaat cggatgttct ggagagcacg
     3301 acgacgagaa tcaatattta ttcaatatat tgtaaagcat catcctagat ttaaagatgc
     3361 gaatattcca atctggttag atcgagattg cccattcaat attgaaggcg gcgatgaact
     3421 tgttttatct aaagatgtct tggctatagg cgtttcagaa cgtacatctg cacaagctat
     3481 tgaaaagtta gcgcgacgta tttttgaaaa tccacaggcg acgtttaaaa aagtagtagc
     3541 aattgaaatt ccaactagtc gaacatttat gcacttagat acagtattta caatgataga
     3601 ttatgacaaa tttacaatgc attcagccat tttaaaggca gaaggcaata tgaatatatt
     3661 tattattgaa tatgatgacg taaataaaga tattgccatc aaacaatcta gtcatttaaa
     3721 agatacttta gaagacgtac taggtataga tgatatccaa ttcattccaa caggaaatgg
     3781 tgatgtcatt gatggtgcta gagagcaatg gaatgatggc tcaaatacat tatgcataag
     3841 accaggtgtt gtagtgactt acgatagaaa ctatgtatcg aatgatttat tgagacagaa
     3901 aggcattaaa gtcattgaaa tatctggtag cgagttggta cgtggacgtg gggggcctag
     3961 atgtatgagt caaccattat tcagagaaga catttaacgt aataaattat agaaacgagg
     4021 gtgaaaataa tgacagaaat tcaaaaaccg tatgatttaa aaggcagatc attattaaaa
     4081 gaaagtgatt ttaccaaagc agaattcgaa ggacttattg attttgcaat tacattaaaa
     4141 gagtataaga aaaacggtat taagcatcac tacttatctg gaaaaaatat tgcactacta
     4201 ttcgaaaaga attcgacgag aacgcgtgca gcgtttacag ttgcgtctat tgatttaggt
     4261 gcgcatccag aatttttagg gaaaaatgat attcaattag gcaaaaaaga atctgtagag
     4321 gatactgcga aagtattagg tagaatgttc gatggtattg aattccgtgg tttttcacaa
     4381 caagctgttg aagatttagc gaagttctct ggtgtaccgg tgtggaatgg attaacagac
     4441 gattggcatc ctacacaaat gttagctgat tttatgacaa taaaagagaa ttttggatat
     4501 ctagaaggaa taaacttaac ttacgttgga gatggacgta ataatattgc gcattcatta
     4561 atggtagcag gtgctatgtt aggtgttaat gtaagaattt gtacacctaa atcattaaat
     4621 ccaaaagagg catatgttga tattgcaaaa gaaaaagcga gtcaatttgg tggttcagtc
     4681 atgattacgg ataatattgc agaagcagtt gaaaatacag atgctatata tacagatgtt
     4741 tgggtatcga tgggtgaaga aagtgaattt gaacaacgta ttaatttatt aaaagactat
     4801 caagtgaatc aacagatgtt tgatttaaca ggtaaagatt caacgatatt cttacattgt
     4861 ttaccagcat tccatgatac aaatacactt tatggacaag aaatttatga aaaatatgga
     4921 ttagctgaaa tggaagttac agaccaaatc tttagaagtg aacattcaaa agtgtttgat
     4981 caagctgaaa atagaatgca tacaattaag gcagtaatgg cagcaacatt ggggagttaa
     5041 tcactaaatg gaacgatatg aatatgatgt gtctgatgat ataagtgtca tgtacagaca
     5101 cctcatattg gtattaaagg agaaatgaat atgaacgaat caggagataa caaactcagt
     5161 aaatcttctt taattggact agttatagga tccatgattg gtggcggtgc gttcaatata
     5221 atgtctgata tgggcggtaa agccggtgga ttagccatta ttattggttg gattattaca
     5281 gctataggaa tgatttcatt agcgttcgta tttcaaaatt taaccaatga acggccggag
     5341 ctagacggtg gtatttatag ttatgctcaa gcaggatttg gcgattttgt aggctttatc
     5401 agtgcttggg gatattggtt ctcagcgttt ttaggcaatg ttgcctatgc aacactattg
     5461 atgtcagcag taggtaactt tttcccgatt tttaaaggag gcaacacatt accaagtatt
     5521 attgtcgcct cgttactact ctggggtgtc catttcttga ttttaaaagg cgttgaaaca
     5581 gcagcattta tcaatagtat tgttactgtt gcaaagttaa taccgatttt acttgtaatc
     5641 atatgcatga taattgcatt caattttgac acttttaaaa caggcttttt cggtatgacg
     5701 tcagagggtg tattgccatt tagttgggcg agcacaatga gccaagttaa aagtacgatg
     5761 ctagtgacag tatgggtgtt tatcggtatc gaaggtgcag taattttttc tagtagagct
     5821 aaaaataaaa aagatgtagg tagtgccacg gttataggac ttatatcagt tttaattatc
     5881 tatttcttat taactgtatt agctcaaggc gtgattttgc aaaatcatat ttctcaatta
     5941 gattcgccaa gtatggcaca ggtgcttgca actattgtag gtggttgggg atctacactt
     6001 gtaaatattg gtttaattat ttcggtacta ggtgcatggt taggatggac actgcttgct
     6061 ggtgaattac ctttcattgt tgcaaaagat ggattatttc caaaatggtt tgctaaagaa
     6121 aataaaaatg gagcacctgt aaatgcactg ctgattacca atatattagt acaattattt
     6181 ttaataagta tgctatttac acagagtgcg tatcaatttg cattttcact agcatcaagt
     6241 gctattttat acccttacat gttcagtgca ttttaccaag ttaaatacac tttagagcat
     6301 cgacagcaag caactactaa acaatggacg attggtatca tagcctcact ttatgctata
     6361 tggcttatat atgcagcagg tatcaattac ttattactga cgatgttact ttatattccg
     6421 gctcttcttg tttattcaat cgttcaaaag aataatcaga cacgtttgat taaatcagac
     6481 tatattcttt ttatgattat tatcgtactt gcagttatcg ggttaattaa gttattgatg
     6541 ggaacgataa atgtttttta aaaggagcga caaaaatatg aaagagaaaa ttgtcattgc
     6601 gttaggcggt aatgcgatac agacaaaaga agcaacagct gaagcacaac aaacagctat
     6661 tagacgtgcg atgcaaaacc ttaaaccttt atttgattca ccagcgcgta ttgtcatttc
     6721 acatggtaat ggcccacaaa ttggaagttt attaatccaa caagctaaat cgaacagtga
     6781 cacaacgccg gcaatgccat tggatacttg tggtgcaatg tcacagggta tgataggcta
     6841 ttggttggaa actgaaatca atcgcatttt aactgaaatg aatagtgata gaactgtagg
     6901 cacaatcgtt acacgtgtgg aagtagataa aaatgatcca cgatttgata acccaactaa
     6961 accaattggt cctttttata cgaaagaaga agttgaagaa ttacaaaaag aacagccaga
     7021 ctcagtcttt aaagaagatg caggacgtgg ttatagaaaa gtagttgcgt caccactacc
     7081 tcaatctata ctagaacacc agttaattcg aactttagca gacggtaaaa atattgtcat
     7141 tgcatgcggt ggtggcggta ttccagttat aaaaaaagaa aatacctatg aaggtgttga
     7201 agcggttata gataaagatt ttgctagtga gaaattagca acgctgattg aagcagatac
     7261 cttaatgatt cttacgaatg tagaaaatgt atttattaac tttaatgaac ctaatcaaca
     7321 acaaatcgat gatattgatg tagcaacact gaaaaaatac gcggcacaag gtaagtttgc
     7381 ggaaggatcg atgttgccca aaatagaagc tgcgatacga tttgttgaaa gtggggaaaa
     7441 caaaaaagtt atcattacca atttagagca ggcatacgaa gctttgattg gtaataaagg
     7501 tacacacatt cacatgtagc actttatcac gcgataaaac attaaatatg tttctccgtt
     7561 gattcaaatg aaaaagttgt ctgctgacac tttgcaaggt ttgaaggagt ttaacttatg
     7621 acagaaaact ttattttggg tagaaataat aaattagaac atgaactaaa ggcattagca
     7681 gattacatta atatcccata tagtatatta caaccatatc aaagtgaatg ttttgtcaga
     7741 cattatacga aaggccaagt tatttatttt tcgccacaag aaagtagcaa tatttacttt
     7801 ttaattgaag gtaacattat tagagaacat tacaatcaaa atggagatgt atatcgttat
     7861 tttaataaag agcaagtatt atttccaatc agtaacttat ttcatccgaa agaggttaac
     7921 gaattgtgta cagcattaac cgattgtaca gttcttggat tgcctagaga attgatggcc
     7981 tttttgtgca aagctaatga tgatatattt ttgacacttt ttgcattaat aaatgataat
     8041 gagcagcaac acatgaacta taacatggca ttaacaagta aatttgctaa agatcgaatt
     8101 atcaaattgt tatgccatct atgtcagaca gtaggatacg atcaagatga attttatgaa
     8161 atcaaacagt ttttaactat tcaactcatg agtgatatgg ctgggatttc ccgggaaaca
     8221 gctggtcata ttattcatga acttaaagat gaaaaacttg ttgttaaaga tcataaaaat
     8281 tggttagtaa gcaaacattt attcaatgat gtatgtgttt aatatacaat gtaaaatgaa
     8341 taagttgaac atgaggtcta acgtacattt atacgttagg ccttttttgt tagcatgatg
     8401 aataatttaa aatgttagtt aaatttgatt gttgaaatta cagtaaaatt taaggtgatg
     8461 aaaaatttag aacttctaag tttttgaaaa gtaaaacatt tgcaatagtg taaaaatagt
     8521 atattgattt ttgctagtta acagaaaatt ttaagttata taaataggga gaaaacaaat
     8581 tttacgtaat ttttttcgaa aagcaattga tataattctt atttcattat acaatttaga
     8641 ctaatctaga aattgaaatg gagtaatatt tttgaaaaaa agaattgatt atttgtcgaa
     8701 taagcagaat aagtattcga ttagacgttt tacagtaggc accacatcag taatagtagg
     8761 ggcaactata ctatttggga taggcaatca tcaagcacaa gcttcagaac aatcgaacga
     8821 tacaacgcaa tcttcgaaaa ataatgcaag tgtagattcc gaaaaaaaca atacgataga
     8881 aacacctcaa ttaaatacaa cggctaatga tacatctgat attagtgcaa acacaaacag
     8941 tgcgaatgta gatagcacag caaaaccaat gtctacacaa acgagcaata ccactacaac
     9001 agagccagct tcaacaaatg aaacacctca accgacggca attaaagatc aagcaactgc
     9061 tgcaaaaatg caagatcgaa ctgttcctca agaagcaaat tctcaagtag ataataaaac
     9121 aacgaatgat gctaatagca taacaacaaa cagtgagctt aaaaatcctc aaacattaga
     9181 tttaccacaa tcatcaccac aaacgatttc caatgcgcaa ggaactagta aaccaagtgt
     9241 tagaacgaga gctgtacgta gtcttgcagt tgctgaacct gtagtaaatg ctgctgatgc
     9301 taaaggcaca aatgtaaatg gtcaagttac ggcaagtgat ttcaagttag aaaagactac
     9361 atttgaccct aaccaaagtg gtaacacatt tatgtcggca aattttacag tgacagataa
     9421 agtgaaatca ggggattatt ttacagcgaa gttaccagat agtttaactg gtaatggaga
     9481 cgtggattac tctaactcga ataatacgat gccaattgca gatattaaaa gtacgaatgg
     9541 cgatgttgta gctaaagcaa catatgatat cttgactaag acgtatacat ttgtctttac
     9601 agattatgta aatgataaag aaaatattaa cggacaattt tcattaccat tatttacaga
     9661 ccgagcaaag gcacctaaat caggaatata tgatgcgaat attaatattg cggatgaaat
     9721 gtttaataat aaaattactt ataactatag ttcgccaatt gcaggaattg ataagccaaa
     9781 tggcgcgaac atttcttctc aaattattgg tgtagataca gcttcaggtc aaaacacata
     9841 caagcaaacg gtatttgtta accctaagca acgagtttta ggtaatacgt gggtgtatat
     9901 taaaggttac caagataaaa tcgaagaaag tagcggtaaa gtaagtgcta cagatacaaa
     9961 actgagaatt tttgaagtga atgatacatc taaattatca gatagctact atgcagaccc
    10021 aaatgactct aatcttaaag aagtaactga tcaatttaag gataaaatca cttataaata
    10081 ccaaaatgta gcaagtatta attttggtga tataactaaa acatatgtcg tattagttga
    10141 aggccactat gataatacag gtaaaaactt gaaaacgcag gttattcaag aaaatattga
    10201 cccagcgaca ggtaaagact acagtatttt cggatggaat aatgagaatg ttgtacgtta
    10261 tggtggtgga agtgctgatg gtgattcagc agtaaatccg aaagatccaa ctccagggcc
    10321 accggttgac ccagagccaa atccagaacc agaaccaagt ccagagccag aaccggagcc
    10381 aacaccggat ccagagccag aaccggaacc aacgccggat ccagaaccaa gtccagaccc
    10441 ggaaccggaa ccaacgccag atccagagcc aagtccagaa ccagatccgg attcggattc
    10501 ggacagtgac tcaggctcag acagcgactc aggttcagac agtgactcgg actcagatag
    10561 caactcggac tcagacagtg agtcagattc agacagcgat tcggattcag attcagatag
    10621 agactcggac tcagacagtg attcagactc agacagtgat tcagactcag acagcgactc
    10681 gaattcagac agcgattcgg attcagacag tgactcggat tcagacagtg actcggattc
    10741 agacagtgac tcggacttag atagcgattc agactcagac agtgactcag attcagatag
    10801 tgattcggat tcagacagcg actcggattc agacagtgac tcggattcag atagcgactc
    10861 agactcagac agtgactcgg attcagacag cgactcgaat tcagacagcg attcggattc
    10921 agacagcgat tcggattcag atagcgactc agactcagac agtgactcgg attcagacag
    10981 tgactcggac ttagatagcg attcagactc agacagcgac tcagattcag atagcgattc
    11041 ggattcagac agcgactccg attcagatag cgactcagac tcagatagtg actcagactc
    11101 agacagtgac tcagattcag acagcgactc agattcagac agcgattcgg attcagacag
    11161 cgattcagac tcagacagtg actcagattc agatagcgat tcggactcag atagcgactc
    11221 cgattcaaga gttacaccac caaataatga acagaaagca ccatcaaatc ctaaagatga
    11281 agtaaaccat tctaataagg catcaaaaca acacaaaact gatgctttac cagaaacagg
    11341 agataagagc gaaaacacaa atgcaacttt atttggtgca atgatggcat tattaggatc
    11401 attactattg tttagaaaac gcaagcaaga tcataaagaa aaagcgtaaa tactttttta
    11461 ggccgaatac atttgtattc ggtttttttg ttgaaaatga ttttaaagtg aattgtttaa
    11521 gcgtaaaatg ttgataaagt agaattagaa aggggtcatg acgtatggct tatatttcat
    11581 taaactatca ttcaccaaca attggtatgc atcaaaattt gacagtcatt ttaccggaag
    11641 atcaaagctt ctttaataac gatacaactg ctaaaccatt aaaaacttta atgttgttac
    11701 atggattatc aagtgatgaa acgacatata tgagatatac aagcatagaa aggtatgcga
    11761 atgaacacaa attagctgtg attatgccca atgtggatca tagcgcatat gctaacatgg
    11821 catatggtca tagctattat gattatattt tggaagtata tgattatgtt catcaaatat
    11881 ttccactttc caaaaagcgt gatgacaatt ttatagcagg tcactctatg ggaggatatg
    11941 gcacaattaa atttgcatta acacaagggg ataaatttgc caaagctgta ccattatctg
    12001 ctgtgtttga agcgcaaaat ttaatggatc tagagtggaa tgatttttca aaagaggcca
    12061 taattggcaa tctttcaagt gttaaaggaa ctgaacatga tccgtattac ttgctagaca
    12121 aagctgtagc tgaagataaa caaattccaa aattgctcat tatgtgtggt aaacaagact
    12181 ttttatatca agacaactta gattttatcg atcatttatc acgcaaaaat gttccttatc
    12241 aatttgaaga tggaccagga gatcatgatt atgcatattg ggaccaagcg attaagcgtg
    12301 ctataacatg gatggtgaat gattaattat ttcctggaaa atatgtgcct gcattaaata
    12361 cacagagtga gtgatacaaa ctatttacgc acgactaaca tttctgagtg tttaaattaa
    12421 ttttgtatta atatgattgg cgcaatttgc tgatacacaa aaatgtttct cgtgaaactt
    12481 agatttagct tatagtttta tcatcatttg tatgacttac attataaatt ttattataat
    12541 gaggttaacg ctttgaaagg agtcatcatc atgtcgacca ataaaaacga ttatgagcat
    12601 atgttgtttt attttgcata taaaacgttt attactaccg ctgatgaaat tatagagaag
    12661 tatggtatga gccgtcagca tcatcgtttt ttgtttttta tcaataaatt acctggtatt
    12721 actattaaat cattactaga aatattagaa atttctaaac aaggatcaca tgcaacactt
    12781 caaaaattaa aagagcaagg tctcattatt gaaaaagttt tagagactga tcgacgtgtc
    12841 aaaaaattat attcgacgga taaaggcgat caactcattg ctgaattgaa caaggcgcaa
    12901 gatgaattat tgcaaaatat atatcaacaa gtcggttcgg attggtatga tgtgatggaa
    12961 gcattggcta aagggcgacc tggctttgat tttattaagc atttgaaaga tgaaaaagaa
    13021 agctagcatc agaaatgtta aaaatcttcg cattcttaaa tttaaaaaat atgtcaaaaa
    13081 gtgtataata aaaacatata atttaattga actcagtttc aacacatctt agaaaggagt
    13141 ttgaatgatg aaaaaattag cagttatttt aacattagtt ggcggtttat actacgcatt
    13201 taaaaaatac caagaacgtg ttaaccaagc acctaacatt gagtactaaa ttaaaccata
    13261 aaaaattccc gaacaccttg ttgtagtgct cgggaatttt tttatgcttt acttgaatat
    13321 atcaaatatt atttttgcgc tttctgtatt ttcgatatta ccactaaatg attctgatct
    13381 aggtccataa gcgtaggtat taacatcttc gcctgtatgt ccatcggaag tccaccctgt
    13441 ataagattta tcatttactg gcttctgaat tgcgtgttgt aaggcttttg tttgtttttc
    13501 tacatctgcg gatttttcgt ctttttcttt tttaagtagt ctttttagct ttttattctc
    13561 ttttttaacc tttttcatat catcttgtga aaattcaaag ccataacctt cattaataac
    13621 tttttcaggg tcttcacctt tagccatttt ttctgtcata tatgatccag agtgtttcat
    13681 cgatttaatc ggttgaggat tccattcgta tcctttatct ttaccaattg ttagaccacc
    13741 agtagagtga tctgctgttg caacgacaag tgtatcttta tgcttcttag catattgaat
    13801 ggcgtcatcg aatgcttttt caaaaccttc catttcagac ataacgcctg taatatcgtt
    13861 ggaatgcgct gatttatcta tagaagcacc ttcgaccatt aaaaagaaac cttttttatt
    13921 acgctcaagc ttattaagtg cactttgttg catatcagct aatgatggtt cgtctttaga
    13981 agcatctatt gcaagtggca tatttttatc tgcaaacaaa ccaagaactt tatctttatc
    14041 agattttgat aactccttac tgttcgtggc aaggtcgtaa ccatcttttt tgaatttttt
    14101 atctaaattg ccattacttt taccgaaata tttagcgcca ccgcctaata aaacatcaac
    14161 tttatgcttt ccgttgattt tatctttata aaattgttta gcgatttcgt ttttatcatc
    14221 tctagaagtc acgtgtgcag catatgctgc tggtgttgca tctgttaatt cagctgttga
    14281 aacaagacca gtcgacttac ctttttcttt tgcacgttca agcaccgtct ttactttctg
    14341 cttgttactg tcaacaccga tggcaccatt atatgtctta tgaccagaac taaaggctgt
    14401 tccgccagct gcagaatcag taatattctg ttttgggtca tttgaatatg tacgatttgt
    14461 gccttttaaa tatgaatcaa aagcagtagg ggtcatttct ttagcatgcg gatcattttt
    14521 ataataacga taagctgtgt taaatgatgg acccatgcca tcgccaacta aaaagataac
    14581 attttttgga tttttagtat taccaaccgc gaaactttca tctttagaac ttttatcgga
    14641 ttgcgcaatt gcaggtgtga cagaactaaa aactgttgac acgataataa ggttagcaac
    14701 tgcaaatttt gtggcttttt taactgataa cataagacat cctcctgagt atatgactat
    14761 gtcttcagtg taaaagagga attttgagca attatgtagt tttagttaaa aatatgtaaa
    14821 cagagtgatt tagaataaca aaaaatgaat atatatgaca atttgttata gaaagcgtta
    14881 gaatagaagc gtgtgaaaat atagaattaa atataatttg aggtggaaaa atgatactag
    14941 taatgttatc tccattatta atcatattct ttatagtgtt gtctatttta gaagagcgta
    15001 aacgtacgaa gaaaaagcaa ctcgagaaag aaaaagcaaa tacactaaat caaaatacaa
    15061 atgatacgga aagttcaaat caagagccgt cattgcagca ggctaaagaa caaaaagatg
    15121 acaaagtata attcaattga aggaagaaga ttatagatga aaatattaat tgttgaagat
    15181 gattttgtta tagcagaaag tttagcatct gaacttaaaa aatggaacta cggtgttatt
    15241 gtcgttgaac aatttgatga tatactgtct atctttaacc aacatcaacc tcagcttgta
    15301 ttgctagata ttaatttgcc aacgttaaat ggttttcatt ggtgtcaaga aatccgaaaa
    15361 acatctaatg tgccaattat atttattagt tcccgtattg ataatatgga ccaaattatg
    15421 gcaatacaaa tggggggaga tgattttatc gaaaaaccat ttaacttgtc attaacgatt
    15481 gccaaaattc aagcattatt gagacgaact tatgacttgt cagtagctaa tgattcaatg
    15541 acggttaaag gttgtacatt aatattagat gaagcaaaag tcgtgtatca agaacaaagc
    15601 atacagctat ctttgactga attacaaata ttaaagttat tatttcaaaa tgaagataaa
    15661 tatgtaagta gaactgcttt aattgaaaaa tgttgggaat cagaaaactt catagatgat
    15721 aacacattag ctgttaacat gacgcgcctg cgtaaaaaat taaatactat tggcgttaat
    15781 gattttatca ttacaaagaa aaatgtcgga tataaagtat agggtgaatg caatgacctt
    15841 tcttaaaagt attactcagg aaatagcaat agtcatagtt atttttgctt tatttggctt
    15901 aatgttttac ctgtatcatt tgccattaga agcatattta ctagcacttg gcgttatttt
    15961 attattatta ctcatattca taggtattaa atatttaagt tttgtaaaaa ctataagcca
    16021 acaacaacaa attgaaaact tagaaaatgc gttgtatcag cttaaaaatg aacaaattga
    16081 atataaaaat gatgtagaga gctacttttt aacatgggta catcaaatga aaacacccat
    16141 tactgcagca caactgttac ttgaaagaga tgagcctaat gttgtgaatc gtgttcgtca
    16201 agaggttatt caaattgata actatacaag tttagcactt agttatttaa agttattaaa
    16261 tgaaacttct gatatttctg tcactaaaat ttcgattaac aatatcattc gcccaattat
    16321 tatgaaatat tcaatacagt ttattgatca aaaaacaaaa atccattatg aaccttgtca
    16381 tcacgaagta ttaactgacg ttagatggac ctctttaatg atagaacaat taataaataa
    16441 tgcacttaag tatgcgagag gtaaagatat atggattgaa tttgatgagc aatccaatca
    16501 attatacgta aaagataatg gtatcggtat tagtgaagcg gacttgccta aaatatttga
    16561 taagggctat tcaggttata atggccagcg ccaaagtaac tcaagtggga ttggtttatt
    16621 tatcgtaaaa caaatttcaa ctcacacaaa ccatcctgtt tcagtcgtat ctaaacaaaa
    16681 tgagggtaca acatttacga ttcaatttcc agatgaataa aaactttcaa tattgtaagc
    16741 atactagtaa cattttttca ctaatttaaa tgttattagt atttttttgt tttaatatag
    16801 aactaacaaa gaaatgaggt gcatgccatg ttgctagaag tgaaacatgt aaaaaaggtt
    16861 tatggtaaag gtttgaatgc tacgacagca cttaatcaaa tgaatttatc agttggagct
    16921 ggtgaatttg ttgcaattat gggtgagtct gggtcaggga agtctacact actaaattta
    16981 attgcttctt ttgatggact aactgaaggt gacattattg tggatggcgc acatttaaat
    17041 aatatgaaaa ataaaagtaa agcattgtat cgtcaacaaa tggtaggttt tgtttttcaa
    17101 gattttaatc ttttaccaac aatgacgaat aaagaaaata taatgatgcc attaatttta
    17161 gctggtgcta aacgaaaaga tatagaacaa agggtccatc agttgacagt acaattacat
    17221 ttagagggat ttttaaacaa atatccttct gaaatctcag ggggtcagaa gcaacgcatt
    17281 gccattgcac gtgcattagt tactaaaccg acgattctac tagccgatga acctacaggt
    17341 gcacttgatt caaaaacatc aaaggcattg atgatgttat ttcaagagat tcatcaattg
    17401 gaacagacaa ttttaatggt aactcattca aatatcgatg cgtcttatgc agagcgtgtc
    17461 atttttatta aagatgggcg tctatatcat gaaatatatc gtggtgaaga aagtcaatta
    17521 gcttttcaac aacgaataac agatagctta gcacttgtga atggaggaag tgtcaatata
    17581 tgaaattaag attgttatgg cacataatgc gacgtcaatt tatcacgcag cgacttgtaa
    17641 tcattccatt cattttagcg gtaagtgtac tattcatgat tgaatatacg cttgtgtcaa
    17701 ttgggttaaa tagctacata aaacagaaga atgacttcct agttccattt attatcatag
    17761 ctaatttttt tatggcgctt ttaactttta tttttatttt ctatgcaaat cactttatga
    17821 tgtcacaaag acgaaaagag tttagcattt ttatgacatt gggcatgacc aagaaaagta
    17881 tgcgtttaat tgtagtgatg gaaactatct tacaatttgt gataatttca gtcgtgagta
    17941 ttgccggcgg atacttactt ggtgcgatat ttttcttgtt tatacagaaa ataatgggca
    18001 gtgaagttgc gacgttaagg tattatccat ttgactctgt agcgatgttt attactttga
    18061 ttatcattgc tgtattaatg ggcatgctac ttatattcaa cttgttcagt attaattttc
    18121 aacggccgat aacttatcaa catcgttccg attccagtgt catatcacga tggttgcgtt
    18181 acgttttaat tgttatagga agcgcagcac tatatttagg ctactttatt gcattacaac
    18241 aagatacgac gtttggtgcc ttttttaaaa tatggattgt cataggatta gttattatcg
    18301 gtacttatgc attttttgta ggtataagtg aaataattat tagtatattg cagcaggtat
    18361 caaaagttta ctatcatcca cggtattttt ttgtggtagt tgggatgcgt gtacgtctta
    18421 aaatgaatgc agtcagtctt gcaacaatca ctttgctatg tacatttttg attgtaacgc
    18481 tcacaatgac attaacaacc tatcgcgata tgaatcatac cattatgaaa ttgattgcga
    18541 atgattatga tttgtcgttt aacgacaatt ctaagtcaca aatagaacgt caacaaatga
    18601 ttgagaatat taagcgtgat atacagggtt cggttaatgt aaaagacttc aaagtatatg
    18661 aaacgacatt atttagagct tctttagaaa agaatcgtgc ttattacaag ttaaaacagg
    18721 ctagtgatga tattccaata gacttcattg gtaatgaagg cgcgatattt tcaagacaat
    18781 cagtaatgat tacagctttt actgcacaag attataatcg atatcaccaa aacaaattac
    18841 acttagacaa tcaaagtata ggtatgatta cgaatgtacc tatttttaaa aagcagtcta
    18901 aggtcggtct taataatgaa gtctttaaag tgaaacaact tgacgcacat gcgttaaatt
    18961 tgatgatgat tcaagatagt atggttttaa ttgttaaaga taataatcaa ttacaaaaag
    19021 taaaaggatt ttatgcacat gaacaaaaga agtcgacaat atcgataaac tttaatgtat
    19081 ttggaaagac gaaattaaca acatcacaaa tacaaaagct atcgaaaaaa tacgatgcgt
    19141 caataaattc taaatcagaa atgctaatgg tttgggatcg tctgacaggt ggactaatat
    19201 ttgtaggtgg cgtagtatca attgtacttg tcattggaat atttctaatg atgtactata
    19261 aacaagtttc agaagcctat gcaaatcagc acaattatga cattatgaaa aaacttggtt
    19321 tagataatgg aagaatagca aagataacac gaaatcaaat gactttcttg ttcgcaatac
    19381 caataacagt agcactgatt catacattaa tttctagtaa cattgtatat acgttactaa
    19441 atatgatagg tattaataac catcatatat ttttaacaag ttacgtaata gctgtcatta
    19501 taatttcgtt tctatatatg gcgatgtaca aaataacttc ctatatttat gctaaagtta
    19561 ttcatcaaca acgcaattaa ccatgtttca gtgaaatcac aatcaat
//
LOCUS       DAHHRR010000040        18942 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.040, whole genome shotgun sequence.
ACCESSION   DAHHRR010000040 DAHHRR010000000
VERSION     DAHHRR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18942
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.040"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            1..1566
                     /locus_tag="NBI42_001265"
     CDS             1..1566
                     /locus_tag="NBI42_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCD1777690.1"
                     /translation="MEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEMI
                     RLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLGA
                     SSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLLP
                     GVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYIL
                     MAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTGP
                     HAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPHL
                     IKDGEIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFFA
                     FTTILSYYYITETNVAYLTRNQNNQVSSVFINIARVIILFATFYGAVKTADVAWAFGD
                     LGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDSNKLPNAVFWLK
                     TYPERLKQARVKK"
     gene            1710..2468
                     /locus_tag="NBI42_001266"
     CDS             1710..2468
                     /locus_tag="NBI42_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCD1777691.1"
                     /translation="MLEFKAGKINKHVLYSDILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFEGGHQWKDWKPLLSDILLYFLSKNTDDKLYE"
     gene            2662..3171
                     /locus_tag="NBI42_001267"
     CDS             2662..3171
                     /locus_tag="NBI42_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HCD1777692.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIE
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(3284..4474)
                     /locus_tag="NBI42_001268"
     CDS             complement(3284..4474)
                     /locus_tag="NBI42_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1777693.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLISWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHIVWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIANR
                     EKAK"
     gene            complement(4452..5627)
                     /locus_tag="NBI42_001269"
     CDS             complement(4452..5627)
                     /locus_tag="NBI42_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCD1777694.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            6059..7543
                     /locus_tag="NBI42_001270"
     CDS             6059..7543
                     /locus_tag="NBI42_001270"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCD1777695.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQSVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLFDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVGQ"
     gene            7524..7784
                     /locus_tag="NBI42_001271"
     CDS             7524..7784
                     /locus_tag="NBI42_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HCD1777696.1"
                     /translation="MALLVNKRLIDEGKTIDVYLFEALNDQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            7784..9346
                     /locus_tag="NBI42_001272"
     CDS             7784..9346
                     /locus_tag="NBI42_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCD1777697.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEEVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     IITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            9648..10451
                     /locus_tag="NBI42_001273"
     CDS             9648..10451
                     /locus_tag="NBI42_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCD1777698.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            10670..12994
                     /locus_tag="NBI42_001274"
     CDS             10670..12994
                     /locus_tag="NBI42_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCD1777699.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKEKHKDSINKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDLVDQDTEKSKYDEQNSEATLSTKSTDKVESTDMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDFESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHTPSISDDKDNVMRENHIVDDNPDNDINAPSLSKTGDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSSASHRSTEKRNMAINDHDKLNGQKPNTKTLANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPIDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            13011..14369
                     /locus_tag="NBI42_001275"
     CDS             13011..14369
                     /locus_tag="NBI42_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCD1777700.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMVLMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            14508..16019
                     /locus_tag="NBI42_001276"
     CDS             14508..16019
                     /locus_tag="NBI42_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCD1777701.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFVVKNR"
     gene            complement(16203..16291)
                     /locus_tag="NBI42_001277"
     tRNA            complement(16203..16291)
                     /locus_tag="NBI42_001277"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16254..16256),aa:Ser,seq:gct)
     gene            complement(16294..16368)
                     /locus_tag="NBI42_001278"
     tRNA            complement(16294..16368)
                     /locus_tag="NBI42_001278"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16334..16336),aa:Asn,seq:gtt)
     gene            complement(16510..17079)
                     /locus_tag="NBI42_001279"
     CDS             complement(16510..17079)
                     /locus_tag="NBI42_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCD1777702.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIENIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMVS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            17288..17506
                     /locus_tag="NBI42_001280"
     CDS             17288..17506
                     /locus_tag="NBI42_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HCD1777703.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTTEYKNLEAFLGE"
     gene            complement(17587..18573)
                     /locus_tag="NBI42_001281"
     CDS             complement(17587..18573)
                     /locus_tag="NBI42_001281"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCD1777704.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVIDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            18772..>18942
                     /locus_tag="NBI42_001282"
     CDS             18772..>18942
                     /locus_tag="NBI42_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2187 family protein"
                     /protein_id="HCD1777705.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIV"
BASE COUNT         6592 a         2668 c         3435 g         6247 t
ORIGIN      
        1 attgaaaaat tagtaacctt tttaaatgag gttgtttgga gtaagccatt agtttatggt
       61 ttgctaatta ctggtgtgct atttacattg cgtatgcgat tttttcaagt tagacatttt
      121 aaagaaatga ttcgattaat gtttcaagga gagaagtctc caaatggtat ttcaagtttt
      181 caagcgatag ccatgtcttt agcaggcagg gttggtacag gtaatattgt cggtgtatct
      241 actgcaatat ttataggagg acctggtgca gtattttgga tgtggattac tgcgttttta
      301 ggtgcaagta gtgcttttat tgaatctaca cttggtcaaa tattcaagag agttgaaaat
      361 aatgaatacc gtggtggacc agcgtattat attgaatatg gtattggtgg taaatttggt
      421 aaaatttacg gaattatctt tgctattgtt acgattatct cagtaggtct gttgcttcct
      481 ggtgtgcaat ctaacgctat agcaagttct atgcacaatg cgattcatgt tccacaatgg
      541 ttaatgggtg gtattgttgt agttattttg ggattaatta tttttggtgg tgtacgtagt
      601 attgccaatg ttgcaacagc cgttgtacca tttatggcaa ttatttacat actaatggct
      661 gtcattatca tttgtatcaa tatacaagaa gtgccagcgt tatttgcatt aatttttaaa
      721 tcagcatttg gattacaatc tgcttttggt ggtatcgttg gcgcaatgat agagattggt
      781 gttaaacgtg gattatactc aaatgaggct ggtcaaggta caggtcccca cgcagcagcg
      841 gcagcagaag tatcacatcc aagtaaacaa ggtctagtac aagcattttc agtatatatt
      901 gatacattat ttgtatgtac tgcaactgct ctgattatac ttatttctgg tacatataat
      961 gtgactgatg gtacggttaa tgcgaatggc acaccgcatt taattaaaga cggtgagatt
     1021 tatgttgaaa atgcaacagg caaagattat tcaggtactg cgatgtatgc acaagccggc
     1081 attgataaag cgttccatgg cagtggttat caatttgatc ctactttctc tggcgtaggt
     1141 tcgtacttta ttgcatttgc tttattcttc tttgcattta ctactatttt gtcgtactac
     1201 tacattacag aaacaaatgt tgcttattta acgcgtaatc aaaataatca agtttcatcg
     1261 gtatttatta atattgctcg tgtgattatt ttgttcgcta cattttacgg tgcagttaaa
     1321 acagctgatg tagcatgggc attcggtgat ttaggtgtag gtctaatggc ttggttaaat
     1381 atcattgcga tttggatttt acataagcct gccgtaaatg ctttaaaaga ttatgaaatt
     1441 caaaagaaac gtttaggcaa cggttataat gcagtttatc aacctgattc gaataaatta
     1501 cctaatgctg tcttttggtt gaagacatat ccagaacgtt taaaacaagc acgtgtcaaa
     1561 aagtaatcta cttttgttta tagtatatgt agtgatcatt tgataaaaaa gaaaaagtat
     1621 tgagaatttt aggtgctcag aaatttgaat tttaaaatat agtgtctctt ggtacaataa
     1681 taatacaatt actagggggc acttttttta tgttggaatt taaagctgga aagattaata
     1741 agcatgtttt atatagtgat attttaaata gagatgtcac actaagtatt tatttgccag
     1801 aatcttataa tcaacttgtt aaatataatg tcattctttg ctttgacgga ttagattttt
     1861 tacgtttcgg gagaatacaa cgtacatatg aatcgttaat caaagaagcc cgtattgatg
     1921 atgcaatcat tgtcggattc cattatgaag acgttgataa gcgtagagag gaatttcatc
     1981 cacaaggaag tcgttctcat ttaactattc aatcagtcgg taaagaaata ttgccattta
     2041 ttgactcgac gttttctaca ctgaaagtag gtaatgcaag gttattagta ggggatagtt
     2101 tagcgggtag tattgcctta ttaacggcgt tgacctatcc aacgatcttt agtcgtgtag
     2161 caatgttaag tccacattca gatgaaaaag tattagataa gctaaatcaa tgtgctaata
     2221 aagaacaatt gacaatttgg catgtcattg gtctagatga aaaagatttc actttaccaa
     2281 caaatggtaa gcgtgccgat ttcttaacac cgaatagaga attagctgaa caaattaaga
     2341 aatataatat aacttattat tacgatgaat ttgaaggtgg tcaccaatgg aaagattgga
     2401 aaccattgct gtcagatata ttattgtatt ttttaagtaa aaacacagat gataaacttt
     2461 atgaataatt tacattagta gatttagtat gaattgtctt catatagtct ggtctataat
     2521 ataatttata aaagatttta ctgtttaatt taatttaaat ttgtcgaaat tgcaaaagat
     2581 gtataatgaa ttatttttaa tgtaacggtt ttcaaagaaa tttgatataa tagcaatagg
     2641 ttaaacaaag gaggaattca gatgatttta ggattagcat taattccatc aaagtcattt
     2701 caagaagcgg tggattctta ccgtaaaaga tatgataaac agtattcacg aattaaacca
     2761 catgtgacaa ttaaagcgcc atttgaaatt gaagatggtg atttagattc tgtcattgaa
     2821 caggttagag ctcgtattaa tggtatacca gcagtagaag ttcatgctac aaaagcttct
     2881 agcttcaaac caacgaacaa tgtgatttac tttaaagttg cgaagacgga cgacttagaa
     2941 gaattgttta atcgctttaa tggagaagat ttctatggag aagctgaaca tgtttttgtg
     3001 ccacacttta caatagcaca aggactatct agccaagaat tcgaagatat ttttggtcaa
     3061 gtagcattag ctggggtaga ccataaagaa attatcgatg aattaacttt gttacgtttt
     3121 gacgatgacg aagataaatg gaaagttatt gaaacgttta aattagctta agtaacataa
     3181 tagtattgtt aatcgtagta tgtttgaatt aataagaaaa tggtcatttt tattgaatgt
     3241 aataaaaatg accattttct ttattttaaa atacgtttta accttactta gctttttctc
     3301 tatttgctat aaagtagctt ccataaaata cagctaagac taaaaagatt aatgccgaga
     3361 aataaaatgt attgtttaaa ttgttggtaa attgtgtaat taatccacca aataatggcc
     3421 cgatcattga gccgaatcct tggatactat taaaaacacc ccaagtttct tcttgttcat
     3481 ctgatttgat aaatcgtgcc ataaaggtat tccatgctgg taataggatg ccatacatta
     3541 gaccgatagc taaagcgata atccacacga tgtgaatatt aacaatcata gatagagtga
     3601 aaattaatat catgtataaa ataaatccgc ttagaatgac accatacata aagtttctgc
     3661 tgcggttatc tattagtttc gataaaaata gcatcgaaac tgcacagccg ataccaccaa
     3721 taatgattgc aacagtatat tcaattgtgc ttacgttaat aaccttagta gcatatgttg
     3781 gtaatatagg aactagggca gcaattgcgg caccttgtaa aagaatacca gggaacaaca
     3841 ataaatggcg ctttgtcaca tcaacaattt gtctcaattg agctttaact ggacgagtat
     3901 tgtaatttgt taactttaca tcgacaaaat aatataatat ccatgaaatt aaaacgacta
     3961 aagacatcat gaaggcaaag cgtgttgggt gcactttgat aagtagattc ataaaaacca
     4021 tacctaccaa taggcctaac aaccatgaaa aataaacata gcccatttgt ttgccacgtt
     4081 tatcttcttc aacactggat aacataatga cccaaatagg actaactgca ataccgagca
     4141 tcatagcact aaatatgatt acaaaaggtg atgctggaaa ccaaataact aaaaataaac
     4201 ttgtaaatgc taaaataaat ccagtcgtta aaacgatttt tgtgccgaat tttttcagta
     4261 aaaagcctat aacaaagttt gtagatgcat cagcaataaa atgtattgaa aatgctagag
     4321 acgttattgc tacagcaatg gatgtaactg ttggcaagaa attaatatag cttaggatat
     4381 acatgcctct cgcaaattcc attaaaaata agataataag cattaaaatg aaatttttat
     4441 gattagcgta attatttaac gaagaatctt gcatataaag gaacctttcc ataaatctct
     4501 tgtggttgtg atgaatgacc gattaaatca agtaagtccc tacatattgt ctgtgtagca
     4561 tacttaattt tatcttgttc cattgtacta atcatgttag ttaattgctc attaccattc
     4621 gttaaactag ctacaatttt tattgcttct tctggagtat cagcgatttt accaaaacct
     4681 ttttcttcaa agtaaagggc attttcaagc tcttgaccag gtgcaggatt taggaaaatc
     4741 attggaatac aacgggcgaa accttcagtt attgtgatac caccaggttt cgtaatcata
     4801 agttgacttg atgccatcca ttcattcatg tgtttggtat aacctagaat caatacattc
     4861 tcgttagatt taaacttagc tgttaaagat cgctttagct ctttgctctt accacaaatc
     4921 ataactactt gtgcatttgc acttttcgct aatatatcag tgatcatcgt gtcaaaacct
     4981 ttagatacac caaatgcacc agctgacatt aaaatagttt gcttatctgg atctaagttg
     5041 ttgtctatta accactgctt ttgattaata ggtgtttcaa atttgttatc aataggaata
     5101 cctgtcactt taactgttga aggatcaata cctacgtcta tgaagtcttg tttcgtttct
     5161 tttgttgcca cataatatct tgttgaatac ggcgtaatcc agtttttatg taagcgatag
     5221 tctgtcatca ctgtagcaac tggaatatta atgttaaatt gctcagttag taccgacata
     5281 actggtgtag gaaacgttaa taatattaaa tctggctttt cttttatcaa taaattaatt
     5341 aatttattaa gtccatagta tttgtagaaa catttgtcta gtttatctgg gcggctgtaa
     5401 taaaaccctt tgtacatatt tctaaaatat ttaaagctat tgatatacca ttttttacaa
     5461 atagaagtca aaattggatg agcttccata aataaatcgt gctcaatgac gcttaaatgg
     5521 tctagattca tatcattaag ttgattaacg atactctgtg taacttgcat atgaccgtta
     5581 ccgaaagagc cagtaataat caatatcttt ttattttgag taaccattaa tagccaccct
     5641 ccgttagttt gaaaatttta tttaagtgta acttatttta cggcattata aaagaaataa
     5701 agacgcaaag tcgttacatt tatagcattt taatcaatag atgaattgat acaaaataaa
     5761 acgttatttt ataaagcaat ttattgttct atgttttatt tgtatattta aaattatcca
     5821 gtatacaatt atagcatatt tttggaaaca attatgatat tataccatgt tacaagatgg
     5881 ttttaataat ttaagatgag ccataattgt gaaactaatt cataatacct tatgttttat
     5941 ttttaatagt agaaattaga aaatgctgat tagtaggata taacagtgaa attataaatt
     6001 tattaacatc aacaaaacgt gtataataaa catattgtag aaaaaggagc ggttcagttt
     6061 ggatgcaagt acgttgttta agaaagtaaa agtaaagcgt gtattgggtt ctttagaaca
     6121 acaaatagat gatatcacta ctgattcacg tacagcgaga gaaggtagca tttttgtcgc
     6181 ttcagttgga tatactgtag acagtcataa attctgtcaa agtgtagctg atcaagggtg
     6241 taagttggta gtggtcaata aagaacaatc attaccagct aacgtaacac aagtggttgt
     6301 gccggacaca ttaagagtag ctagtattct agcacacaca ttatttgatt atccgagtca
     6361 tcagttagtg acatttggtg taacgggtac aaatggtaaa acttctattg cgacgatgat
     6421 tcatttaatt caaagaaagt tacaaaaaaa tagtgcatat ttaggaacta atggtttcca
     6481 aattaatgaa acaaagacaa aaggtgcaaa tacgacacca gaaacagttt ctttaactaa
     6541 gaaaattaaa gaagcagttg atgcaggcgc tgaatccatg acattagaag tatcaagcca
     6601 tggcttagta ttaggacgac tgcgaggcgt tgaatttgac gttgcaatat tttcaaattt
     6661 aacacaagac catttagatt ttcatggcac aatggaagcg tacggacacg cgaagtcttt
     6721 attgtttagt caattaggtg aagatttgtc gaaagaaaag tatgtcgtgt taaacaatga
     6781 cgattcattt tctgagtatt taagaacagt gacgccttat gaagtattta gttatggaat
     6841 tgatgaggaa gcccaattta tggctaaaaa tattcaagaa tctttacaag gtgtcagctt
     6901 tgattttgta acgccttttg gaacttaccc agtaaaatcg ccttatgttg gtaagtttaa
     6961 tatttctaat attatggcgg caatgattgc ggtgtggagt aaaggtacat ctttagaaac
     7021 gattattaaa gctgttgaaa atttagaacc tgttgaagga cgattagaag ttttagatcc
     7081 ttcgttacct attgatttaa ttatcgatta tgcacataca gctgatggta tgaacaaatt
     7141 aatcgatgca gtacagcctt ttgtaaagca aaagttgata tttttagttg gtatggcagg
     7201 cgaacgtgat ttaactaaaa cgcctgaaat ggggcgagtt gcctgtcgtg cagattatgt
     7261 cattttcaca ccggataatc cggcaaatga tgacccgaaa atgttaacag cagaattagc
     7321 caaaggtgca acacatcaaa actatattga atttgatgat cgtgcagaag ggataaaaca
     7381 tgcaattgac atagctgagc ctggggatac tgtcgtttta gcatcaaaag gaagagaacc
     7441 atatcaaatc atgccagggc atattaaggt tccacatcga gatgatttaa ttggccttga
     7501 agcagcttac aaaaagttcg gtggtggccc tgttggtcaa taaaagactt attgatgaag
     7561 gtaaaactat tgatgtttat ttattcgaag cattaaatga ccagataatc attgctatac
     7621 cagattggtt ttggtcatat cagatggcaa tgacattaga tgaagaaact tgttttgaag
     7681 caatactcat gcaattgttt gtttttaaag aagaggaaga ggcagaatcg attgcatcac
     7741 aactaacaga ttggatagaa acatataaaa aggagaaaga ctaatgaact taaagcaaga
     7801 agttgagtct agaaagactt ttgcgattat ttcacatccc gatgcaggga aaacaacgtt
     7861 aactgaaaaa ctattgtact tcagtggtgc cattcgtgaa gcgggtacag ttaaagggaa
     7921 gaagactggt aaatttgcga caagtgactg gatgaaagtt gaacaagagc gtggtatttc
     7981 tgtaacaagt tcagtaatgc aatttgatta cgatgattat aaaatcaata tcttagatac
     8041 accaggacat gaagactttt cagaagatac gtatagaaca ttaatggcag ttgacagtgc
     8101 tgtcatggtc atagactgtg caaaaggtat tgaaccacaa acattgaagt tatttaaagt
     8161 ttgtaaaatg cgtggtattc caatctttac attcattaat aaattagacc gagtaggtaa
     8221 agaaccattt gaattattag atgaaatcga agagacatta aatattgaaa cataccctat
     8281 gaattggcca attggtatgg gacaaagttt ctttggcatc attgatagaa agtctaaaac
     8341 cattgaacca tttagagatg aagaaaatat attacatttg aatgatgatt ttgagttgga
     8401 agaagatcat gcaattacaa atgatagtgc ttttgaacaa gcgattgaag aattaatgtt
     8461 ggttgaagaa gcgggtgaag cctttgataa tgacgcgctg ttgagtggag acttaacacc
     8521 tgtatttttc ggttcagctt tagctaactt tggtgtacaa aatttcttaa atgcatatgt
     8581 tgattttgcg ccaatgccaa atgcgagaca aacaaaagaa gaggttgaag taagcccgtt
     8641 tgatgattca ttttcaggat ttatctttaa aattcaagcc aacatggacc ctaaacaccg
     8701 tgatagaatt gcctttatgc gtgtcgttag tggtgcattt gaacgtggta tggatgttac
     8761 tttgcaacgt actaataaaa agcaaataat cacacgttca acgtctttta tggcagacga
     8821 taaagaaact gtgaatcatg ctgtagcagg cgatatcatt ggactatatg atacaggtaa
     8881 ttatcaaatt ggagatactt tagttggtgg aaaacaaacc tacagtttcc aagatttacc
     8941 acaatttacg ccagaaattt ttatgaaagt ttctgctaaa aacgttatga aacagaagca
     9001 tttccataaa ggaattgaac aattagtaca agaaggtgcg attcaatact ataaaacatt
     9061 acacacaaac caaattattc taggtgctgt tggtcaatta caatttgaag tattcgaaca
     9121 tcgaatgaaa aatgaataca acgttgatgt tgttatggaa ccagttggtc gtaaaattgc
     9181 acgatggatt gaaaatgaag accaaattac agataagatg aacacatcaa gatcgatttt
     9241 agtgaaagat agatatgacg atttagtatt tttatttgaa aatgaatttg caacaagatg
     9301 gttcgaagag aaattccctg aaattaaatt gtatagttta ctttaacggc tcaattgtat
     9361 aatcgaattt gttacattaa aaataattgt ttcgttgaag aaaaataaat tgtatatttt
     9421 aaaagaaaaa ggtatactat gatgtatcaa atgaataacc tatggcattt tgtcagaggg
     9481 gagtaactta agaatcatga ccgtataaat gattcgacac tttatcgtca ttacgaagat
     9541 atcttccggt aaagtgggca atttaaattg cttagtgaga cctttgctat ttatttagca
     9601 taggtctttt tgtttgtact taacttattt atttaaagga gttgtacatg ttaatggatc
     9661 caagtttgat cttaccttat ttatgggtac ttgtcgtttt agtattttta gaaggcttat
     9721 tggcagcaga taacgcgatt gttatggctg taatggttaa gcacttacca cccgaacaac
     9781 gtaaaaaagc tttgttttac ggtttgttag gtgcatttgt atttagattt ttagcattat
     9841 tcttaattag tattatcgcg aacttttggt ttattcaagc tgcaggagcg gtttacttaa
     9901 tttatatgtc aatcaaaaat ctgtggcagt tctttaaaca cccagaaatt gaaagtcctg
     9961 aagctggaga tgatcatcat tatgatgaat ctggtgaaga gattaaagca agtaacaaat
    10021 cattctgggg aactgtgttg aaaatagaat ttgcagatat cgcatttgcc atagattcta
    10081 tgcttgctgc tttagctatt gctgtaacac ttcctaaagt tggtattcac tttggtggta
    10141 tggacttagg tcagttcgta gtcatgttcc taggtggaat gattggtgtt attctaatgc
    10201 gttatgcagc aacatggttc gtagagctat taaacaaata tccaggactt gaaggtgcag
    10261 ccttcgcgat cgttggttgg gtaggtgtta agttagttgt catggtatta gcgcacccag
    10321 acattgctgt attgcctgag cacttcccac atggcgtatt atggcaatct attttctgga
    10381 cagtactaat tggattagta attatcggtt ggttaggttc agttgttaaa aataaaaaat
    10441 cacataaata attgatgtga agcggacaat cttaatttag tttaaggttg tcctttttca
    10501 tttaattgag tgatttatga aaaatgaatt ttgaagaata tgaatcaaaa gatgcgatat
    10561 agcattaaga aaatgtgcct tttatattta gcattttttc aatagaaatt atatagattt
    10621 taaagcaaat taagtgctaa tgtgtcataa tgaaaagtga ttttattgaa tggagtggac
    10681 attagtggat attggtaaaa aacatgtaat tcctaaaagt cagtaccgac gtaagcgtcg
    10741 tgaattcttc cacaacgaag acagagaaga aaatttaaat caacatcaag ataaacaaaa
    10801 tattgataat acaacatcaa aaaaagcaga taaggaaaaa cataaagatt caattaataa
    10861 gcacgaacgt tttaaaaata gtttatcatc acatttagaa cagagaaacc gtgatgttaa
    10921 tgagaataaa gctgaagaaa gtaaaagtaa tcaggatagt aagtcagcat ataacagaga
    10981 tcattattta acagacgatg tatctaaaaa acaaaattca ttagatttag tggaccaaga
    11041 tacagagaaa tcaaaatatg atgagcaaaa ttctgaagcg actttatcaa ctaaatcaac
    11101 cgataaagta gaatcaactg acatgagaaa gctaagttca gataaaaaca aagttggtca
    11161 tgaagagcaa catgtacttt ctaaaccttc agaacatgat aaagagacta gaattgattt
    11221 tgagtcttca agaactgatt cagacagctc gatgcagaca gagaaaataa aaaaagacag
    11281 ttcagatgga aataaaagta gtaatctgaa atctgaagta atatcagaca aatcaaatac
    11341 agtaccaaaa ttgtcggaat ctgatgatga agtaaataat cagaagccat taactttgcc
    11401 ggaagaacag aaattgaaaa gacagcaaag tcaaaatgag caaacaaaaa cctatacata
    11461 tggtgatagc gaacaaaatg acaagtctaa tcatgaaaat gatttaagtc atcatacacc
    11521 atcgataagt gatgataaag ataacgtcat gagagaaaat catattgttg acgataatcc
    11581 tgataatgat atcaatgcac catcattatc aaaaacaggt gacgatcgaa aacttgatga
    11641 aaaaattcat gtcgaagata aacataaaca aaatgcagac tcgtctgaaa cggtgggtta
    11701 tcaaagtcag tcaagtgcat ctcatcgtag cactgaaaaa agaaatatgg ctattaatga
    11761 ccatgataaa ttaaatggtc aaaaaccaaa tacaaagaca ttggcaaata ataatcaaaa
    11821 aaaggctaca tcaaaattga acaaagggcg cgctacaaat aataattata gtgatatttt
    11881 gaaaaagttt tggatgatgt attggcctaa attagttatt ctaatgggta ttattattct
    11941 aattgttatt ttgaatgcta tttttaataa tgtgaacaaa aatgatcgca tgaatgataa
    12001 taatgatgca gatgctcaaa aatatacgac aacgatgaaa aatgccaata acacagttaa
    12061 atcggtcgtt acagttgaaa atgaaacatc aaaagattca tctttaccta tagataaagc
    12121 atctcaagac gaagtaggat caggtgttgt ttataaaaaa tctggagata cgttatatat
    12181 tgttacgaat gcacacgttg tcggtgataa agaaaatcaa aaaataactt tctcgaataa
    12241 taaaagtgtt gttgggaaag tgcttggtaa agataaatgg tcagatttag ctgttgttaa
    12301 agcaacttct tcagacagtt cagtgaaaga gatagctatt ggagattcaa ataatttagt
    12361 gttaggagag ccaattttag tcgtaggtaa tccacttggt gtagacttta aaggcactgt
    12421 cacagaaggt attatttcag gtctgaacag aaatgttcct attgatttcg ataaagataa
    12481 taaatatgat atgttgatga aagctttcca aattgatgca tcagtaaatc caggtaactc
    12541 gggtggtgct gtcgtcaata gagaaggaaa attaattggt gtagttgcag ctaaaattag
    12601 tatgccaaat gttgaaaata tgtcatttgc aatacctgtt aatgaagtac aaaagattgt
    12661 aaaagattta gaaacaaaag gtaaaattga ctatccggat gtaggtgtta aaatgaagaa
    12721 tatcgccagt ctaaatagtt ttgaaagaca agcagttaaa ttgccaggaa aagttaagaa
    12781 cggtgttgtt gtagatcaag ttgacaacaa tggtttagcg gatcaatctg gtctgaaaaa
    12841 aggtgatgta attacagaat tagatggcaa acttttagaa gatgatttac gctttaggca
    12901 gattatattt agtcataaag atgacttgaa atcaattaca gcgaagattt atagagatgg
    12961 taaagagaaa gaaattaata ttaaactaaa ataatgttga ggtgaacaac gtgtcaattt
    13021 ttagccagtt tttaaaaaga tcaagccctc aacaaggtat tgtattgtac tatatcgtcg
    13081 caattgtcat tgcattttta ttattaaact taccgtatgt tcataaacca ggtgtagaag
    13141 taaatccaat tgacacatta tttgttgccg tatccggaat tagtgttact ggattgtctc
    13201 cgataagtat tgtcgatacc tattctacat ttggacaatt aattatcctc gtgatattaa
    13261 atattggtgg aattggcgtc atggcaattg gtacgatgtt atgggtggta ctaggtaaac
    13321 atattggaat tcgagaacgt cagttaatta tgttagataa taacaaaaac acaatgagtg
    13381 gtaccgtcaa attgattatt gatattgtaa aatcaatatt tgtaatcgaa ctcgtaggag
    13441 ccatgttatt agcattttac ttttatcgag ataatccaga tttaaaatat gcaatcatgc
    13501 aaggtgtttt tgtttctatt tctgccacta ccaatggtgg attagatatt acaggtaagt
    13561 cattaattcc ttatgcacat gattattttg tacaagcgat agttatattt ttaataattt
    13621 taggatcaat cggcttccca gtattattag aagttaaagc ttatattcaa aatagggtta
    13681 ctaattttag attttcatta tttactaaaa ttacgacatc aacatactta ttcctattta
    13741 ttgttggggt attagccatt ctattatttg aacataacca tgcgttcaaa ggattaagtt
    13801 ggcatcaatc gttgttctat tcactgtttc aatcagcgac tacaagaagt gcgggtcttc
    13861 aaacaattga tgtgacaaca ctaagtgacc ctacgaatat tatcatgggt attttaatgt
    13921 ttataggttc ttcgccaagt tcggttggtg gcggtattcg tacaacaaca ttcgctattt
    13981 taattttgtt tttaattaac tttagtaata atgccgataa aacatccata aaagtataca
    14041 atagagaagt acacattatg gatattcaac gttcatttgc agtatttaca atggcgacaa
    14101 ttttaacatt tttaggaatg ctaattatat cagctactga aaatggtaaa cttacatttt
    14161 tacaagtatt ttttgaagtc atgtctgcat ttggaacttg tggactatcg cttggtgtca
    14221 caagtgatat tagtgatatt tccaaggttg tactaatggt actcatgttt ataggacgtg
    14281 ttggcttaat atcatttatc attatgatag caggacgtcg agaaccagat aaattccatt
    14341 atccaaaaga acgtattcaa ataggataat ataatagcaa tctaagttta gttaatgtag
    14401 attttaactg gaacttagat tgctttttta gtttgtattt ttaacttatt ttataagacg
    14461 attggttttg aaaatggtaa actagtaaca atgagaggtg taacatgatg gaaaaaaatg
    14521 aaaacattaa tgtagagatt ttaactacgt cagatatgca tagtcatttc ttaaatggtg
    14581 attatggttc aaatatttat agagctggta cttatgttaa ccaagtaaga gcacaaaatc
    14641 atcgcgtcat tttattagat agtggcggaa gtttagctgg ctcgttagcg gcctattatt
    14701 atgctattgt tgcaccttat aaacgacatc caatgataaa gttaatgaac agaatgcatt
    14761 atgatgctag cggtgtgagt ccaagtgaat tcaagtttgg tttatcattt ttaactcgtt
    14821 caattgcttt ggcacgtttt ccatggttat cagcaaatat tgaatacaat gttactaagg
    14881 agccttattt ttcaactcca tattgtatta aacattttgg tgacttaaaa attgctatcg
    14941 taggcgtcac agcagatggt ttaatggaaa atgagtattc tgaaatggag caagatgtat
    15001 ctattgaaaa gacattagtg gcatcaaaac gttggattag atatatccat gaagttgaag
    15061 agccggattt tttgattgta atttatcatg gtggattgaa taaaattagt aatagcacga
    15121 aaaataaaaa ggcaagttcg aatgaagctg aaaaattaat ggaagaactc ggtgttatag
    15181 atttaatgat tacagctcat cagcatcaaa caatagtagg tcaagatcat gaaacgtatt
    15241 atgttcaggc tggtcaagat gcaaaagagc ttgtacatct ttcgattaat tttaaaaaga
    15301 gaacaacaac ttatgatgtt gaaagcattg attctaaagt gattgactta aatgaatatg
    15361 aagaggatca agaattatta gatttaacat tctatgatag aaaagcagtg gcttattggt
    15421 cacaggaaat cataagtgat aaaggtttga tgttatcagt aaatgggtta caagatttag
    15481 tctgtcaaac acatccattt tcgcaattat tacatgatgc aattcacctt gcatttgata
    15541 atgatataac atgtgtccac gtgcctatga acggagagaa ggggttgagt ggacagattc
    15601 gaaatgaaga tttgtatcat gcatacccat atccagataa gccaatggat atgacaatta
    15661 gtggtcaaaa tatcaaagat atattggagt atagttattc acatttagat tttgttaacg
    15721 agcaattaag cttaacaatt attgatgaaa cgttatgtac aatgtggcaa ggattcaatt
    15781 atgaaattga tatgaatcaa gaacctgggc aacgagtaat gttagatcaa attgatttga
    15841 ctaagagtta tagagttaca atgactgact attgttatcg taactacaag aattatttaa
    15901 aaaatgctat tatacatgaa tcatacgatg aaacaatgag tacattaatt gcagagaagt
    15961 taagagatcc gaattatcgt atttcatgta gtgataattt tgtagttaaa aacaggtaaa
    16021 tattgctatg taataaatta attataagtt taacaagtat aaaaaccttt cttagagaag
    16081 attatctagg aaaggttttt agaattttaa aattgatata aaagaacagt gaaattctca
    16141 ttcattatat atatataagt aaacaaaaaa gcttaaatgg tatataccat ttaagcaagt
    16201 aacggaaacg gagggattcg aaccctcgcg ccgctttcgc gacctacacc cttagcaggg
    16261 gcgcctcttc agccaacttg agtacgtttc caatggctcc acaggtagga ctcgaaccta
    16321 cgaccgatcg gttaacagcc gatagctcta ccactgagct actgtggatt aatttgtatg
    16381 tgtttgaata tcaagcacaa atattattat aacaagatta agttaatttg caatagatac
    16441 cttaaaaaaa tatgcagaac atttacacta tatttataaa acattccgca tattttaaaa
    16501 ataaacaatt tatttatctt cgaataggga atagcgtgtc aatgcttcaa atacgtttaa
    16561 tgtggcttta ttataatcta taggttgatc gggattttta gaaaccatgc gagattgttt
    16621 atctaccata tagtagtaaa aaatggaatt gttatattgg tgccataaac tgtggtatga
    16681 aacatgaaga ataatagatt cgttattaat aaaagttact ttacatttac gattttttaa
    16741 ttccttaata ttttctatat aatgcatatt taaccaaata ttttcatttt gtctgtcaga
    16801 atgtgttggg aaaaaataag ttggaaataa tggtgttagt aaaataggtg gtttacttga
    16861 aatgccagtg atgcggtttg tttctgcttt tttgcctagg taagtattac cataaaattt
    16921 gcaagaacgt tcaatgattt tctggacttt gaatggattt tgtatacgcg ttttatcgaa
    16981 ccggataatt tcgctaccat ttctttgatc atcatcatca aatgctggtc gaataaccat
    17041 gtctccttta cgtattacat aaatattttg agaatacata agcaaaacct cgctttatta
    17101 agttttaaat cattaatata aaatgcaatt aatgatataa agatatttta taataaaatt
    17161 acgagttgtc aagttattat tttggtgaaa atgtacaata atttttgact aatagtcaga
    17221 atattcattg atttcaaaat tagaaaaatg tatacttatt ataaattaaa gaaaggtagt
    17281 gatttctatg aaatacaata ctaatgttaa acatacaact ttagaagcat ttgtcacaac
    17341 cgtcaatgat ttgggtattg aattaattat caatgaagca ctccgagagg taagaaaacg
    17401 acagctcata gaacttatag atgacgcact cgtcaataaa gatgaagcag catttaatca
    17461 atatacgaca gaatacaaaa atttggaggc atttctcggt gaataacatt gattcgaatt
    17521 cgataacggt atcataaaag gactattctt aacatctcat acatgtaaga atagtccttt
    17581 aatcgtttat gacattaatc taattaattc atgtctatct atgtcaccga aatagtgata
    17641 taaatcatat tctgatagtg cttcttctat gtgctcaaag tcgtgtagac agccaactaa
    17701 tgcattttca agatcaatga catctccgac accaaagaaa tcaccgaaaa tttttgcatg
    17761 ttcgatttta cctcgtttaa catcaaactt gatttgtaca aatccttttt caaatttttc
    17821 ctcgcgttca aagttatatt tagggtttct tccataattc cattcccaag ttctatattt
    17881 gtcgttactt aacttttcaa tattttccca atcttcatcc gttaatttat attcttctac
    17941 ttcagtttct ccaaagatag ttttcaagat gattttttta aattcttcaa tttctaaagg
    18001 gtcatttaaa aattcttgaa tgtttgctac tcgtttacga acagatttaa taccttttga
    18061 tttaatctta gctggattaa cttttagtgc attctgaact tcgtctaaat cactatttaa
    18121 catcaacgta ccatgactaa acattctatt tttaacttta accatggcat ttcctgagat
    18181 tttcgcttgt ccaacttgaa tatcgttacg accagttaat tcagcattta cgcctaaaga
    18241 ttgtaatgct tgaacaattg gttcagtgaa tttttgaaaa ttgtggaaac tgttaccatc
    18301 atcatctgtt ataaaactaa aatttaaatt gccagtatca tgataaacag cgccaccacc
    18361 agaaattctt ctaaccacat cgatgttatg agcatctata tatgtctgat ttacttcctc
    18421 tatcgtattt tgattctttc caacaataat agatggtcta tttatgtaaa ataaaaagta
    18481 actttcttct gctggtaaat tttttaaaac atattcttcc attgctaagt ttaaagttgg
    18541 atctgtaata ttattattac taatgaattt cattacaatc tctcccttat atctatatat
    18601 attctctact tatttatgcc ttaactttgc tcaaaatgcc aattatatac tcacaaatca
    18661 ttctaaatat cggcttaaag ttacatttta ttatttttga ttgttaatag tttatttgtt
    18721 aagtgaattt tagtacaata taatttagtt atgatttaag gaggacttaa aatgactgtt
    18781 gcagaagtgg gtaacattgt tgagtttatg gatggattaa gaggtcgtgt tgaaaaaatc
    18841 aacgataact ctgttattgt tgacttaaca attatggaaa attttaatga ccttgattta
    18901 ccggaaaaaa ctgttatcaa tcataaacga tataagattg tt
//
LOCUS       DAHHRR010000041        18438 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.041, whole genome shotgun sequence.
ACCESSION   DAHHRR010000041 DAHHRR010000000
VERSION     DAHHRR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18438
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.041"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..1120
                     /gene="scpA"
                     /locus_tag="NBI42_001283"
     CDS             <1..1120
                     /gene="scpA"
                     /locus_tag="NBI42_001283"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HCD1777706.1"
                     /translation="LSVTPIANAESNSNIKTKDKKHVQVNVEDKSVPTDVRNLAQKDY
                     LSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEGNIDYIVTISPKV
                     TKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVVTQNHKAKLVLKTPRLE
                     DKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYVNKLENFRIRETQGN
                     NGWCAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQRFQFTGLTPREMIYFGQTQG
                     RSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGHAMAVVGNAKLDNGQEV
                     IIIWNPWDNGFMTQDAKNNIIPVSNGDHYQWYSSIYGY"
     gene            1151..1477
                     /locus_tag="NBI42_001284"
     CDS             1151..1477
                     /locus_tag="NBI42_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCD1777707.1"
                     /translation="MEQFELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHKS"
     gene            complement(1771..1944)
                     /locus_tag="NBI42_001285"
     CDS             complement(1771..1944)
                     /locus_tag="NBI42_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HCD1777708.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQNDGTVEV
                     SHQEIIFVGKKIQ"
     gene            complement(1925..2527)
                     /locus_tag="NBI42_001286"
     CDS             complement(1925..2527)
                     /locus_tag="NBI42_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCD1777709.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(2797..3618)
                     /gene="nadE"
                     /locus_tag="NBI42_001287"
     CDS             complement(2797..3618)
                     /gene="nadE"
                     /locus_tag="NBI42_001287"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCD1777710.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVSPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(3611..5080)
                     /locus_tag="NBI42_001288"
     CDS             complement(3611..5080)
                     /locus_tag="NBI42_001288"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCD1777711.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENDDGTYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLAQGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            5264..6340
                     /locus_tag="NBI42_001289"
     CDS             5264..6340
                     /locus_tag="NBI42_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HCD1777712.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVVDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPNDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRKVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVRDPNFFYKKQESHANQCPFHH"
     gene            6360..7154
                     /locus_tag="NBI42_001290"
     CDS             6360..7154
                     /locus_tag="NBI42_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCD1777713.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNLFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFAYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            complement(7334..8896)
                     /locus_tag="NBI42_001291"
     CDS             complement(7334..8896)
                     /locus_tag="NBI42_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCD1777714.1"
                     /translation="MVYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(9102..10199)
                     /locus_tag="NBI42_001292"
     CDS             complement(9102..10199)
                     /locus_tag="NBI42_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HCD1777715.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNHGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGHRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKNCSFEGFVDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNLKHKDVLIVGNTFKNSTQ
                     SIHLEDIDTVFLSPIEAGIQVTTINVDEIKK"
     gene            10571..11131
                     /locus_tag="NBI42_001293"
     CDS             10571..11131
                     /locus_tag="NBI42_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD1777716.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            11184..12113
                     /locus_tag="NBI42_001294"
     CDS             11184..12113
                     /locus_tag="NBI42_001294"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCD1777717.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDKFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(12400..12558)
                     /locus_tag="NBI42_001295"
     CDS             complement(12400..12558)
                     /locus_tag="NBI42_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777718.1"
                     /translation="MNVVAINFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTRRCK"
     gene            12610..13989
                     /locus_tag="NBI42_001296"
     CDS             12610..13989
                     /locus_tag="NBI42_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCD1777719.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKNARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGLIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(14109..15137)
                     /locus_tag="NBI42_001297"
     CDS             complement(14109..15137)
                     /locus_tag="NBI42_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCD1777720.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKSLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            15407..15808
                     /locus_tag="NBI42_001298"
     CDS             15407..15808
                     /locus_tag="NBI42_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCD1777721.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(15865..16038)
                     /locus_tag="NBI42_001299"
     CDS             complement(15865..16038)
                     /locus_tag="NBI42_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777722.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
     gene            16489..17529
                     /locus_tag="NBI42_001300"
     CDS             16489..17529
                     /locus_tag="NBI42_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCD1777723.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLVRTISQTNNNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYHPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            complement(17586..18428)
                     /locus_tag="NBI42_001301"
     CDS             complement(17586..18428)
                     /locus_tag="NBI42_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCD1777724.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNISSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSPTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
BASE COUNT         6274 a         3216 c         2667 g         6281 t
ORIGIN      
        1 tttaagtgtt acccctattg caaacgctga gagcaattca aatatcaaaa caaaagataa
       61 gaagcatgtg caagttaatg tcgaggacaa gagtgttcct actgacgtac gtaatttagc
      121 gcaaaaagac tatctttctt atgttacttc acttgataaa atctataata aagagaaagc
      181 ctcgtataca cttggtgaac cttttaaaat ttataaattc aacaaaaaga gtgatggtaa
      241 ttattatttt ccagttttga acactgaagg taatatcgat tatatagtta caatttcacc
      301 aaaagtcact aagtattcta gttcatcttc taaatacaca atcaatgttt ctccattttt
      361 atctaaagtc ttaaatcaat ataaagatca acaaattact attttgacaa actcaaaagg
      421 ttactatgtg gttactcaaa atcataaagc taaactagta ctaaaaacac cacgtctaga
      481 agataaaaag ttaaaaaaga ccgagagtat acctactggc aataatgtaa cgcaactaaa
      541 gcaaaaagct tctgtcacta tgccaactag tcaatttaaa agtaataatt atacttacaa
      601 cgagcaatat gtaaataaac ttgagaattt caggataaga gaaactcaag gtaacaatgg
      661 ttggtgtgca ggctatacga tgtctgcatt actaaatgcg acttataata ctaacaaata
      721 tcatgcagaa gctgtgatga gatttttaca cccaaactta caaggacaac gattccaatt
      781 tactggttta acgccaagag agatgattta ttttggacaa actcagggaa gaagtccaca
      841 acttcttaat agaatgacta catataatga agttgacaat cttactaaaa ataataaagg
      901 aattgctgtt ttaggtagta gagtagaatc aagaaatggt atgcacgcag ggcatgccat
      961 ggcagttgtt gggaacgcta aattagacaa tggacaagag gttataatta tatggaatcc
     1021 atgggataat ggttttatga ctcaagacgc taaaaacaat ataattccag tatccaatgg
     1081 cgaccattat caatggtatt catctattta tggttattaa aatttgtatt ttagaaaggt
     1141 aaataatatt atggaacaat ttgaattatt tagtattgat aaattcaaat gtaattcaga
     1201 ggctaagtat tatcttaata ttattgaggg agaatggcat cctcaagatt taaatgatag
     1261 ccctttaaaa tttattctca gtacctcaga cgattctgat tacatttgca aatatataaa
     1321 tacagaacac aaacaactca cattatataa taaaaataat agctcaattg ttattgaaat
     1381 atttatacca aatgataata aaatactact aacaattatg aacacagaag ctttgggaac
     1441 ttctcctaga atgactttca ttaagcataa gtcataagac tatctagata tgattaagta
     1501 aactattaat cttattaaag gtctctagga agaaaaagtt tcatgcgatg agattgtttt
     1561 agaaaaagcg acgatatata taatctaaca ataattttta aaatattaag aacccccagc
     1621 atatgctttt tatatagctt gctgggggtt tctcatacaa tatggatcga gcatcttgca
     1681 tgtataaaca aataggctat tcaaagtaaa attactcaaa ctttgaatag cctttcctct
     1741 tcaaatagtc tttatttaaa ttggattatg ttattggatt ttcttaccta caaaaatgat
     1801 ttcttgatgt gatacttcaa ccgtgccatc attttgctct tgaaacacgg gtttttcaat
     1861 acgttttacc ggcatatacc cttgcatttt cattcgtgac aaacaatcag caattgtttc
     1921 gttctcattt actttgaatt tcatgttcta ctacactttc cagttcttct ggttcataat
     1981 ctttaagctt tctacgacga atgcctttag tccagaatcc accatgtatg tttcgaggtt
     2041 catacgcaat gataaatgct ttcggatcta aattttttat cgtatccatc aatttacgtt
     2101 catattttct tggtgttaaa atttgcatca ccatacgact accatctcta ccaaacgcag
     2161 catagtgcgt aacgccatat cctaaatttc gaagttcatt cggtaaatct aactcatatt
     2221 ctgctgaagt tacatttaca actgtataac ctaatgccag tttttcttct attttcatac
     2281 caacaatgat acctattgaa aaaccaaatg cgtaggcaat aatattttga atatggtcta
     2341 aattagacat aaccaaacct aaaccaacga tataaactaa tacttctaaa aaactaactg
     2401 atgcagcaat ataacgataa cctttcaacg ttaaaattgt tcgcatcgtt aaaaacgtta
     2461 cataacaaac gttaatgata aatatagtta gtaccattaa ccatggattt tctgttacga
     2521 atgacataag gcgccccact ctctttcaat tacactttaa ataccaaact gcttcaaact
     2581 aaccttaatg aatgcatatt aatataatat agtacgaaaa ctctcataaa taaatagcga
     2641 atttgtcata aataaaaatt tattcttaaa tgaacatact tacttttgtt catacacttt
     2701 caatttatat actgaagtag cctagctgaa tgtggtttaa cgttattaat tctaactcat
     2761 atttaattta agtcacacgt tagaagaaaa attaaattag gattttggcc acgtgtatct
     2821 tgtatatgca agttcacgtt tgtgtgcatt tcgtatataa tgattttcaa ttactttttg
     2881 ttcttctggc gaaactggct taccttctaa ataattatca atcgcctcat aagttacacc
     2941 taatgcatct tcatctggaa gttgtggttt atcatcttct aaatcagcag ttggcgtttt
     3001 ttcatataat tcctttggcg caccaagata cgctaataat tgacgacctt gtcgtttatt
     3061 caaaccaaat ataggtgcga tatctgcagc accatcacca tacttcgtat aaaaaccagt
     3121 tatattttca gctgaatgat ctgttcctac tacaatacct tgtcggtttg aagcaattga
     3181 aaattgtact ttcatacgtt cacgcgcttt ttcatttcct ttttggaaat ctgtaagaac
     3241 aataccggct tcttttaatg attgcacact ttgatcaact gcaggtttaa tatttacagt
     3301 aactatttcg tctggttcaa tgaatcgcaa agcttgctca acttcatcag catctttttg
     3361 aactccataa ggtaatttaa ctgcaataaa cgtacaatca atgccttctt cacgtaattc
     3421 gttaacagac atttgtacta attttccaac taatgtagaa tcttgtcctc ctgaaatacc
     3481 taacactaaa gattttataa atgaatgtga ttgtacataa ttttttataa attgctttaa
     3541 ttccataatt tcttcagcac tatcgatacg ctttttcact ttcatttctt gtacaataac
     3601 gtcttgtaat ttactcatta tcttcttcca tctccttaac gtgttccgca acttcaaaaa
     3661 tacgtttatg tttattatcc caacatgcct tgcttaaatc gactggatat tcttgtggat
     3721 taaggaaacg cttattttca tcccaaatag attgtaatcc ttgtgctaaa tattcacgtg
     3781 attcatcttc tgttggcatt tgatatacta atttaccatt ttcataaata ttatgatgca
     3841 aatcaatggc ttcgaaagat tttataaatt tcattttata agtatgcact ggatggaata
     3901 attttaaagg ttgttcatcg tatggatttt cattttccaa agtaatataa tcgccttctg
     3961 ctttacctgt tttcttgttt ataatgcgat atacattttt cttaccggga gtcgtaactt
     4021 tttctgcatt gtttgataat ttaatgcgat cgctatatgt gccatcatca ttttcaatcg
     4081 caacaagttt atatactgca cctaatgctg gttgatcgta tcccgtaatc agctttgtac
     4141 caacgcccca agagtctact tttgcacctt gtgctttcaa actcgtaatc gtttcttcat
     4201 ccaaatcatt agacgcgata attttagttt cagtaaatcc tgcttcatca agcatacgtc
     4261 ttgcctcttt agataaataa gcgatatctc cagaatccaa tcgaatacct acaaagttaa
     4321 ttttgtcacc taattctttt gcaactttta ttgcatttgg cacgccagat tttaaagtat
     4381 ggaatgtatc tactaggaac acacaatttt tatgtctttc agcatatttt ttgaaggcaa
     4441 catattcgtc tccataagtt tggacaaatg cgtgtgcatg tgtaccagac acaggtatac
     4501 caaataattt acctgcccta acattacttg tagaatcaaa gcccccgatg taagcagctc
     4561 tagcgcccca caatgctgca tcaatttctt gcgcacgacg tgtaccaaac tccattaatt
     4621 tatcatttga tgcaatttga cgaattctac tagcctttgt tgtaattagt gtatggaaat
     4681 ttacaatgtt taataaaatt gtttctatta attgcgcttg aatcaatggt gcttctacgc
     4741 gtaacaatgg ttcgttacca aagcataatt cgccttcttg catcgaacgg atgcttcctg
     4801 tgaattttaa atcttttaaa tatgataaga aatcatcctt gtagccaata gactttaaat
     4861 attccaaatc agattctgaa aatccaaaat gttctataaa atcaatgacg cgttttaaac
     4921 cattaaaaac agcataaccg ctattaaatg gcatttttct aaaatacaaa tcaaatacag
     4981 ccattttttc atgaatatta tcattccaat aactttcagc catatttatt tgatataagt
     5041 cattatgtaa cattaaactg tcgtcttcta attggtacac ttgtatctct ccaatcgacc
     5101 taaatatttt cttacatttt atcataattc atttttttat atacataaga gccccttaat
     5161 ttccatactt ttaattaaaa tcaaccaaca atttaatgac atatacataa tttttaagag
     5221 tattttaata atgtagacta taatataaag cgaggtgttg ttaatgttat ttaaagaggc
     5281 tcaagctttc atagaaaaca tgtataaaga gtgtcattat gaaacgcaaa ttatcaataa
     5341 acgtttacat gacattgaac tagaaataaa agaaactggg acatatacac atacagaaga
     5401 agaacttatt tatggtgcta aaatggcttg gcgtaattca aatcgttgca ttggtcgttt
     5461 attttgggat tcactcaatg ttgttgacgc gagagatgtt actgacgaag catcgttctt
     5521 atcatcaatt acttatcata ttacacaggc tacaaatgaa ggtaaattaa aaccgtatat
     5581 tactatatat gctccaaatg atggacctaa aattttcaac aatcaattaa ttcgctatgc
     5641 tggctatgac aattgtggtg atcctgctga aaaagaagtt acacgcttag caaatcactt
     5701 aggttggaaa ggaaaaggta ctaattttga cgtgttacca ctgatttacc aattacctaa
     5761 tgagtcagtt aaattttacg aatatcctac ttcattaatt aaagaagtac ctattgaaca
     5821 taatcattat ccaaaattaa gaaaattgaa cttaaaatgg tacgcagtcc ctatcatttc
     5881 caatatggac ttaaaaatcg gtggcattgt gtatccaact gcacccttta acggttggta
     5941 tatggtaact gaaattggcg tacgtaactt tattgatgat taccgttaca atttactaga
     6001 aaaagttgca gatgcttttg aatttgatac acttaaaaat aattcattta ataaagatcg
     6061 agcacttgtt gaactgaact atgctgtgta tcattctttt aaaaaagaag gcgtatccat
     6121 tgttgatcat ttaactgctg cgaaacaatt cgaactattc gaacgtaatg aagcacaaca
     6181 aggacgcaaa gtaaccggaa aatggtcttg gctagcgcca ccattatcac cgacacttac
     6241 gtcaaattat catcatggct atgacaatac agttagagat cctaactttt tctataaaaa
     6301 gcaagaatca catgctaacc agtgcccttt ccatcattaa gtagtttgag gtgtagcata
     6361 tgcaattata ttatttaggt cctaaaggaa cattttcata tttggcttgt cgtcagtatt
     6421 tttctgaaaa tgaagcaact tttcaaccaa agtcaaattt atttgaagtc atcaaggcag
     6481 ttgcagatga tgacacatcg attggtgttg tccctattga gaattctata gaaggaacaa
     6541 tcaacattgt ggcagatgca cttgcacagc aagatgtctt tgcacacggt gaaatacgtt
     6601 tagatattaa ctttgcatta tatggtaatg gaacagattc catttctgat attaaaaaag
     6661 tgtattctat tgcaccagcg atcagtcaaa caacaaatta catacatcaa catcaatttg
     6721 cctatgatta tgtcgacagt acaattcaaa gtttgactaa aattgaaaat ggcgttgcag
     6781 caattgcacc tttaggtagt ggcgaagctt atggttttac acctattgat acacatattg
     6841 aagactatcc acataacgtg acacgttttt tagttataaa aaatcagcag caatttgatc
     6901 aaaatgcgac atcattgatg tttttaatta caccaatgca tgataaacct gggttgttag
     6961 caagtgtgct taatacattt gctttgttta atattaattt atcctggatt gagtctcgtc
     7021 ctttaaaaac acaattaggt atgtatcgat ttttcgttca ggctgattct gcaattacaa
     7081 ccgatataaa gaaagtcatt gctattttag aaaccttaga tttcaaagta gaaatgattg
     7141 gtgcttttaa ttaaacttta ttttctaggt tctacaattt aaataaaata aagagtcaac
     7201 caaggattaa aataggaatc catccttggt tgactttctt ttgttagtat tgtttatata
     7261 ttggtgagtt tatgaacatt ctcaactagt tctttacatg ctaatgatac ttttttattc
     7321 taaactgcat ttcctatttc aaaggcagtg gttgatttat atcaatacct aaaactatag
     7381 gcattacata atacacgact aaaataataa ttattgcact gattaagttt acccagaatc
     7441 caactgatgc catttgtttg atagatattt tacctgaacc aaagataatt gcattcggtg
     7501 gtgtccctac tggtaacatg tatgcacagt tagccgccat agctgcaggt gccataagta
     7561 gtaatggatg cactccaaca gcaacagaca acgttgctaa aatcggtaaa atcatcgttg
     7621 cagttgcggt attagatgtc acttcagtta aaaataagac aaagatcgtt atgacaatta
     7681 caataagaat cggactaaca ccatttaatg atttcaactg ttcgcctaac cattttgcta
     7741 aaccactttc tgatatacct ttcgctaatg ctaaaccgcc accaaataaa attaatacac
     7801 cccaagggag ctcttttgca acttcccagt caatgatacg acgatgtttt tcagtatttt
     7861 tagctggaat aataaataat aatattgata taaaaatagc aatcgtacca tctgcaacag
     7921 atgacgtaac ttcccatttt ttcagaagaa actctcttgt aatccataat aagctggcaa
     7981 gtacaaagat agtttgtact accttttctt catacttcat tttgccaagc tcgtctaact
     8041 tttgtttaat taacgtctga ccaccaggta aatatttcaa atcatgtcta aacgcaacat
     8101 atcttaaata gagccaagta atacctaaca aaacaatgac agttggaatc cctacaatca
     8161 tccatttagc aaaactaatt tcatgtccaa aatgttgcat gtattgtccc tttaaaataa
     8221 ttagcggcgg cgttccgata agtgtgccta atccgccaat cgtacctgca tagccaattg
     8281 ctaaaactag agatttttca aacttttgaa tacttgtttg attcgtattg gcttcttgta
     8341 aatcatgtgc ttccttaata attgctaaac caatcggaat catgatcatt acagctgcag
     8401 tgttcgatac aaacatagat aagaatcctg ttgccaccat gaatccaagt aatatttttg
     8461 aagtgcttgc accaattaaa ttaataattg tcaacgctac tctcgtatgt aaattccatc
     8521 tttccattgc aattgccaaa ataaatccac ctaaaaacaa aaagataata tcattgccat
     8581 attcggatga tacttgttct ggtgtaagta tatgacctaa tggtaataac acaattggta
     8641 ataagctcgt tgctgcaata ggaattgctt cagtaatcca ccaagtcgca atccataaag
     8701 taatcgctaa gacatagacg cctttccaag gtaagtcttg tggatgaaag aataataatg
     8761 ttaataggaa aagtaatgga cctaatatta gtccaataag ttgtcccgca ctaaacggtt
     8821 tctttttctt tgattttgaa aagaatgtta aatacctttt cgatatcatt gattgatgtt
     8881 gattgaaata aaccataaat ttccccctca tgatgattat gacaagctac ttcaactcac
     8941 tatattagag tatagaaagt tgtctatctt gtattatcat taccgtcaaa ataccccttt
     9001 tatcagataa ttggatttat tatagcatat ttataccgtt ttatgaatac gctttcaaaa
     9061 aattatctac tattcttaat cctaatgtca tgcgaaactt tttacttttt tatttcatct
     9121 acattgatag tagtaacttg aatacccgct tcaataggac ttaaaaacac tgtatcaata
     9181 tcttccaaat gaattgattg agtcgaattt ttgaatgtat tcccaacgat taatacatct
     9241 ttatgtttaa gattattata attacgtaca tgtatcgcat ctttagacat tgatccttta
     9301 aattcatttc caattatatt catatttatt cctgcttggg aacctaagtc ttttcctgtc
     9361 atcacatctg cagcgttttt accatctcta actcctaaat atctaatacc accctcacag
     9421 ttaataaact tattattaat aatgaaagca tctttatact tcaagggagt taatgcataa
     9481 ccttgtatat cttcaaatat attatttcta atatgaatat tctcatagta acgattatgt
     9541 ctacttgcat gtgagccaat agctcgattc caacttccca tttcgggcaa ttctgaaggt
     9601 ccaaaataac aatcttcgat aatgacattt ttcgttatcg tgccatctgt cgttccgaat
     9661 tttggaaaag caccaggtac ttgaatgtct aattgtattg cttcagaata aaagcgttcg
     9721 ccactatagt ctacgaatcc ttcaaatgaa cagtttttaa tatagagtcc attaatccca
     9781 caagcatcaa ttgcatgacc actcactaca ttcttaatgg ttacacctat taattgaata
     9841 tcttcagcat gcccaatgca catagctgta ttgttataag gatacgatac gccattcata
     9901 tcaaacttgc cgcctttaat atgaatatga ctgtgtccat tataaccacg ataaaaacca
     9961 aagcgatgac cattttttaa taaaggaccc gaatgacatc ggcgtaaaat agtttcatta
    10021 tccaacaaaa gtgttgtatt gccatatatc gttaatggtt tgcaaatatc atacgtccct
    10081 tttggtatat aaactgtcgt tggtttacat cttccatgat ttaacgcacg ttgtattgct
    10141 cgagtatctt tagttttgtt tatgcctgtt aacccataat ctttcatatt gatatacata
    10201 aatcgtcatc ctttgtctat tttttaatta ctcatatatt aaccaattat tcaaattata
    10261 atcgtgttag gttgatttag cagatagtca taaatctcgt gatgccatct attagcattt
    10321 ttatattcaa aacatcaaca cttgatatta gttctaagtt tgtcacattt agttttctat
    10381 tacagctatt ataatactat cgtaaaaact tcttatatta tttgttgatt gcgtgatttg
    10441 ctcttccaaa aaagttgatt aatctatata cttaatctat atttatatat taaccattag
    10501 ggttaaaaat tactctagca tttatgaata gatgggagtt tattttatta ttatatagga
    10561 gagatgttga atgacacatc gcgcactatt agttgttgac tattcatatg actttatcgc
    10621 agacgacggc ttactaacat gcggtaaacc tggacaaaat attgaagatt ttattgtttc
    10681 tcgtatcaat gactttaatt attatcaaga ccatatattc tttttaatgg atttacatta
    10741 tttacatgac attcatcatc ctgaaagtaa attattccca ccacacaata tcgtagatac
    10801 aagtggtaga gaattatacg gtaaagtagg taaattatac gaaacaatta aagcgcaacc
    10861 taatgtacat ttcattgata aaacgcgcta tgattcgttc tttggtaccc cgcttgatag
    10921 tttattgagg gaaagaagta ttaatcaagt cgaaatcgtt ggtgtatgta ccgatatttg
    10981 tgtgttacat acagcaattt ctgcatacaa cttaggttat aaaatttcag tacctgctga
    11041 gggtgtagct tcatttaatc aaaaagggca tgaatgggca cttgcacatt ttaaaaactc
    11101 attaggtgca gaggtagaac aacacgttta aatcgtgcta aaataatttt aaagaataca
    11161 atatacaagg gagatatttg acaatggcta aaacatatat tttcggacat aaaaatccag
    11221 acactgatgc aatttcatct gcgattatta tggcagaatt tgaacaactt agaggcaatt
    11281 caggtgctaa agcataccgc ttaggtgatg tgagtgcaga aactcaattt gctttagata
    11341 aatttaatgt tcctgctcca gaattactaa cggatgattt agatggtcaa gatgttattt
    11401 tagttgacca taatgaattc caacaaagtt ctgatacgat tgcatctgct acaattaagc
    11461 atgtaattga tcatcacaga attgcaaatt tcgaaactgc tggtccttta tgttatcgtg
    11521 ctgaacctgt tggttgtaca gctacaatcc tttacaaaat gttcagagaa cgtggctttg
    11581 aaattaaacc tgaaattgct ggtttaatgt tatcagcaat tatctcagat agtctacttt
    11641 tcaaatcacc aacatgtacg caacaagatg ttaaagcagc tgaagaatta aaagatatcg
    11701 ctaaagtcga tattcaaaaa tacggcttag atatgttaaa agcaggtgct tcaacaactg
    11761 ataaatcagt tgaattctta ttaaacatgg atgctaaatc atttactatg ggtgactatg
    11821 tgactcgtat tgcacaagtt aatgctgttg accttgacga agtgttaaat cgtaaagaag
    11881 atttagaaaa agaaatgtta gctgtaagtg cacaagaaaa atatgactta tttgtacttg
    11941 ttgttactga catcattaat agtgattcta aaattttagt tgtaggtgct gaaaaagata
    12001 aagttggcga agcattcaat gttcaattag aagatgacat ggccttctta tctggtgttg
    12061 tttctcgaaa aaaacaaatc gtacctcaaa tcactgaagc attaacaaaa taaattccat
    12121 acgtaactgc tcaatttttg acagtatata atttaacaac agttcattaa agtagatata
    12181 actttacttt taatgagctg tttttttgta tatcagatag taatagacac atttgtaaaa
    12241 tgagagatat gtatatacat taccaattta agattacgaa ctaattgtaa ttttagctat
    12301 ctcctgatgt taactttagc actgctactt gatcttaatg aatataatat atagtaattt
    12361 gtgtaacttt acttcgacat tcttatacct tttaaacctt tatttacaac gtcgagtata
    12421 aatttttgtt ttcttcctac ctttttcgtt atgattaaag ttataaatat tattatgtac
    12481 acgattcatc gctctatttt caactttcaa catatataat tcgaaagacc atttaaaatt
    12541 aatggccaca acattcaaat caattaatca ctttttccaa aacaatcata taaggaggtt
    12601 cttttcatta tgaatatcat tgagcaaaaa ttttatgaca gtaaagcttt tttcaataca
    12661 caacaaacta aagatattag ttttagaaaa gagcaattaa agaagttaag caaagctatt
    12721 aaatcatacg agagcgatat tttagaagca ctatatacag atttaggaaa aaataaagtc
    12781 gaagcttatg ctactgaaat tggcataact ttgaaaagta tcaaaaatgc ccgtaaggaa
    12841 cttaaaaact ggactaaaac aaaaaatgta gacacacctt tatatttatt tccaacaaaa
    12901 agctatatca aaaaagaacc ttatggaaca gttttgatta ttgcaccatt taactatcct
    12961 tttcaactag tgttcgaacc tttaatcggt gctattgcag caggtaatac ggcaattatt
    13021 aaaccatctg agttgacacc aaatgttgca cgagtgatta aacgattaat caatgaaaca
    13081 tttgatgcaa attacattga agttattgag ggaggaattg aagaaacgca aacgttaatt
    13141 cacttacctt ttgactatgt cttctttaca ggaagtgaaa atgtaggcaa aatcgtttat
    13201 caagctgcca gcgaaaattt agttcctgtg acattagaaa tgggcggtaa atctccagtc
    13261 atcgttgatg aaacagcgaa tattaaagtt gccagtgagc gcatttgttt tgggaaattc
    13321 actaatgccg gccaaacatg tgttgcacca gattacattt tagtacacga atctgtaaaa
    13381 gatgatttaa tcacagccct atcaaaaacg ttgcgtgaat tttatggtca aaatatacaa
    13441 caaagtccag attatggcct cattgtaaac cttaaacatt atcatcgtct gacgtcatta
    13501 cttaacagtg cacaaatgaa tattgtattt ggtggtcata gtgatgagga tgaacgttat
    13561 atagaaccaa cattgttaga tcacgttaca agtgattcag caattatgca agaagaaatt
    13621 tttggtccta tcttaccgat tttaacgtat cagtcattgg atgaagcaat agcctttatt
    13681 caccaaagac caaaaccttt gagtttatat ttatttagcg aagatgaaaa tgctacacaa
    13741 cgtgtaataa acgagctatc atttggcggc ggcgctatta atgatacatt gatgcaccta
    13801 gcgaatccta aattaccatt tggtggtgtt ggcgcctcag gtatgggacg ctatcatggt
    13861 aaatattcat tcgacacttt tacacatgaa aaaagctaca ttttcaaatc tacacgatta
    13921 gaatcaggtg tccatttacc accatataaa ggtaaattta aatacatcaa agctttcttt
    13981 aaaaattaat ttttgagaca aaaataaacc atgcttccat aatttgatgt tcttgaaaac
    14041 aataaatttc aaggtataca caaatttttt ggaaacatgg ttttcttttt atcatgatta
    14101 attaaagatt aaaatatgac acatacacct tcagatgcta cacgagtgtt atcacatagc
    14161 gtaattttac ctgtttcttt atttctttcg aaaacagtta caactgaatc tccttgctca
    14221 tgagcacaaa ctaaaaggtc atctgatgag gcaatattaa aatctcttgg gaattgacca
    14281 ccactttcag taattgttac tagttctaag tgttgaccat tatcaagaac tttaaaaatt
    14341 gcaatgctat catgccctct attagataca tataagaatt gttgatcatg agataaacgc
    14401 actgctgcaa gtttagtatc tccatcaaag ttttcaggaa ttgttaaatg acgctcgagc
    14461 tcttcaaatt taccgtcatt atattctgca acactcacag tatttgataa ttcgtgtacg
    14521 acataagcaa attttccatt atcatgaaat tcaatatgtc ttgtcccatc actatcttta
    14581 aataaagatt ctttataaaa ttcaaacccg ttgtcatcaa atttataagt aacgatacga
    14641 tcagcaccta aatctgttac tgcaacgtac ttaccatctg gagtttgatt aatataatgt
    14701 gcgtgtggat gatcttgtct ttcatgtgta cctgttggaa aatcatgtgc aagttcttga
    14761 attagacgta taatttcacc tgtgtgcgta tttaattcat acatacgtat gatgccagca
    14821 ccatataccg cttcaaataa atatcgttta tcttctgaaa tcgatacgta acaacctgta
    14881 ccagcttttg aagacaaact tttatttaat aaatgcaatt caccattgtc atcaattttc
    14941 aaactcgcaa caccgcattg ttctccttct ttgttgattc catataaaac ttcgttatta
    15001 cgcaccaaat atgtagacgc ttctaattca aatcctgttt ctaataaatc aatacgtgac
    15061 tgattttcgt ttaattcaaa acgataaatc cctttaccat tctttttagt gtaagaacca
    15121 atatatccat ttgtcattac ttttacccct ccatgttagt cttattttca ttttattaaa
    15181 ttttagctag aattaacaat catttatcat ttccttaaag ctaaaactgt ttaactactt
    15241 taaaagaact atttgctgtg atatgtcatt attcgatttt aattaatccc tcttttttac
    15301 ttacacgagc attaattatt aacgcgttta tgcttccaca aaatttttgt tgcaaaatag
    15361 aacacacgtt cgtataatgt aattatcaga ttggggtgaa ttaaggatga caagttatga
    15421 agaagaaact gattatcgta acgttccaag agaattatta gataacaata tacctatggg
    15481 aagaggcatg attaaatggg ctccttttgc aacattaccc gaacaattcg aaacgattca
    15541 acaatacatt atcgaccaaa acaaaattac acgtcctgta ttatcagatg atcaattggc
    15601 tgagctaaac attcggctac acgaggcgct acaatatgcc caacctgtag aagttaaatt
    15661 ttataataat ggctttgtag attcagtacg cttaaccatt tatcgtattg atgctattaa
    15721 ttatgaaatt gatggttatg tttacaatca gcaacaacgt caaaaaattt ctatctttga
    15781 tatcttagat attgtcttgt taccttaatg ctttgaacat aattaaaggc aagaacaatc
    15841 tatattgtcc ttgcctaaat gttattagtt atttttactt tcgatatcag aaataatttc
    15901 tttagttttt ctttcaatat cttcaaaatc ttttttaaag atataagcaa atgctactac
    15961 accaacacca gcaactaatg ttgtaatagc aactaagcta aatacgaatt taaacacaca
    16021 tttaataaaa ttccacatct caatttcacc tctgttaaat taaataccta tctacatttc
    16081 attatactat catatataga ataatcatta aataaatacc cacttttacg aaatatattc
    16141 atcaaattat atagcgagca agctaatgat tattttcaaa aatgccgagg aacctctgtt
    16201 tcaacgtgac atgaccttat atgctaatgt taacaatgac tattatcgtg aatgctaact
    16261 taagattgat atttatgata agatgatttt aagtttttaa cttaatatcc ccaccatcta
    16321 taaaaatatg ttttaactga ttgataagtc ttatacaccg agaaatcacc tcaaacaatt
    16381 ttatatttca tttagtaaaa tataacttta tttaacatga cattcaatat acatacgatt
    16441 actatttatt tacaatatat tacaactaaa aagaaagggt ccgatgatat gcaacaagtg
    16501 acatcagata ttatgacttt tagaggttca catttcgagt taggtgttaa aactggtaaa
    16561 tggcttcaac aaacacctct gctaaaaaat cgagaaaaag aatggaaaaa acgtgtccca
    16621 cgtttcgata tagatgtaaa tgaaacttat caaatattcc aaacatacgc acctcaaata
    16681 tgggaagaat taatgggact acaaagtata ctcaaaatgc caacacgcca aattatttta
    16741 aactttggac attatcgttt taccgattta aaagaaagtg gttgcacagt ctttcaaggc
    16801 aaagacttca tggttagaaa ttatgattat caccctgcaa cttatgatgg tcgctactta
    16861 ctatatcaac ctacagatag cggtttagct caaattggtc ctgtatcaag ggtaactgga
    16921 agaatggatg gtatgaatga gtcaggatta actatgggct ataactttat gcaccggaag
    16981 aaaccagcaa atggttttgt atgttatatg attggtcgtc taattcttga aaattgccga
    17041 aatgtaacag aagcaatcca attattgaaa gaaataccac acagaagctc atttagctac
    17101 atactgatgg acaaatcatt gaaccatgca attgttgaag tgacgccgag atcaatagat
    17161 gttcgttatg ataatatatg tacaaatcat tttgaaatat taacacatga aaatcgcaat
    17221 tatacaaaag aatctaaaga aagactagtg cgtaccattt cacaaactaa taataattta
    17281 gatatgacta cagcatttaa gttattcaat aatcctcagt atgaaattta cagtaaacta
    17341 ttcaaaagct ggtctggcac catccataca tcaatgtatc accctgaaac attaactgca
    17401 tattttacat tgggagaaaa tgcgccacca gaaataatag atttcaaatc atggttagat
    17461 ggtcaagacc ttaatatcac tcactttact ggtaatatag ataccgactt aacttttgcc
    17521 aacaaataaa taacaccgat attagagaaa ttatgcttcc taatatcggt gcttacatcc
    17581 tttatttatt caactttgat attaccatca atcgtataaa attctaaaac attgtcgctt
    17641 ttcccaactt taccattagt aaatgtttta tttttaacta cgctatcacc cgttcccgga
    17701 tttagcttta gtatagtatc ttcaggttta ctatcatact taaaacgaat atctccttgt
    17761 ttagtagaag cttttgcatc tacttttgat ggcatgtttt taaattcaat gtcaccatga
    17821 tcgtttaaaa atatggattg tttaatttga ctcttgtcga tttgtagcgt accaataaca
    17881 actttaatat tactattttt aatgttactt ttactaatat aagtaggact attatttgtc
    17941 tttgaatcta gcgcgtcaag atttgagttt ttaataacta cttcaccgtt aatgctttga
    18001 atacttgtgt tctctaaatc aacatcactg attctaacac ttcctgctcc agacgatata
    18061 tttaaacgtt taatcatttt atcaggcatt tcaatcgtta acgttttctt attctcatga
    18121 aaaggattca tatcaattgc atatcctctt gtcttagacc ttttatcact aatcttcaac
    18181 gtcttatcta ctatattaat taatatatca ttgtcaccat catatttaac tctaaattga
    18241 ttcccccgtt ttatacgaag ttctgtacta tccaaattta tagatacatt gtcaaaagca
    18301 tcgtctttga atgttttatc atattgttta gtaccatatt tgtttttatc atagctgaac
    18361 caaataatgg ttgctgctgt aaaaaagaca acaaacacta aaagcccaat aaaaaagaat
    18421 ttcttcattt tctaacgc
//
LOCUS       DAHHRR010000042        18302 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.042, whole genome shotgun sequence.
ACCESSION   DAHHRR010000042 DAHHRR010000000
VERSION     DAHHRR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18302
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.042"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(48..770)
                     /locus_tag="NBI42_001302"
     CDS             complement(48..770)
                     /locus_tag="NBI42_001302"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCD1777725.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIEPTPKATTPYQQYQ
                     HIADVKDADVAIDFSNPNLLFPLLDEEFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            complement(767..1654)
                     /locus_tag="NBI42_001303"
     CDS             complement(767..1654)
                     /locus_tag="NBI42_001303"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCD1777726.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNLEALKAHVNFLLENNTQAIIVN
                     GTTAESPTLTTDEKELILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPSIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGKGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGILLSALSVDINPIPIKALTSYLGFGNYELRLPLITLED
                     TDTKVLREAYNIFKAGENE"
     gene            complement(1656..2645)
                     /locus_tag="NBI42_001304"
     CDS             complement(1656..2645)
                     /locus_tag="NBI42_001304"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCD1777727.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTEARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGINGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLEKEATAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            complement(2709..3914)
                     /locus_tag="NBI42_001305"
     CDS             complement(2709..3914)
                     /locus_tag="NBI42_001305"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCD1777728.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKTPKQQELALLLTTGEQQTVSYLSMVLNDIGVNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            complement(4527..6128)
                     /locus_tag="NBI42_001306"
     CDS             complement(4527..6128)
                     /locus_tag="NBI42_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1777729.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNVSFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            6275..7177
                     /gene="cvfB"
                     /locus_tag="NBI42_001307"
     CDS             6275..7177
                     /gene="cvfB"
                     /locus_tag="NBI42_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HCD1777730.1"
                     /translation="MALDKDIVGSIEFLEVIGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDHLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNMKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKITLTKKGWSRIDSKE"
     gene            7642..8625
                     /locus_tag="NBI42_001308"
     CDS             7642..8625
                     /locus_tag="NBI42_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCD1777731.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIIEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            8816..9742
                     /gene="pstC"
                     /locus_tag="NBI42_001309"
     CDS             8816..9742
                     /gene="pstC"
                     /locus_tag="NBI42_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCD1777732.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            9744..10661
                     /gene="pstA"
                     /locus_tag="NBI42_001310"
     CDS             9744..10661
                     /gene="pstA"
                     /locus_tag="NBI42_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCD1777733.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNIA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            10708..11559
                     /locus_tag="NBI42_001311"
     CDS             10708..11559
                     /locus_tag="NBI42_001311"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCD1777734.1"
                     /translation="MAQTLAQTKQISQSHTFDNSQSHHTTPNDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            11566..12207
                     /gene="phoU"
                     /locus_tag="NBI42_001312"
     CDS             11566..12207
                     /gene="phoU"
                     /locus_tag="NBI42_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCD1777735.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(12347..14161)
                     /gene="pepF"
                     /locus_tag="NBI42_001313"
     CDS             complement(12347..14161)
                     /gene="pepF"
                     /locus_tag="NBI42_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCD1777736.1"
                     /translation="MSQGLPLREDVPVSETWDLTDLFKDDQQYYESIESLLQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDCLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSRLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQLLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFKDLKISVDPDYEPEISIEDSKKYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYRKVDQGESLNAPTLNEIMISVYKQFFGDAVEMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAIDAWLETLKKGGSVSPVELAK
                     IAGVDITTDQPLKTTIQYISDLVDEVEKLTDEIEKANN"
     gene            14368..14712
                     /locus_tag="NBI42_001314"
     CDS             14368..14712
                     /locus_tag="NBI42_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777737.1"
                     /translation="MTLFDMPNYLWITTLIMILLTVFCCLVLNKWFVSAVITFVVLGV
                     LAFFIPNFQEIKYQPLLGYAAFLAIMSLIISFLLWYFTRNWRKDRKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            15043..16029
                     /locus_tag="NBI42_001315"
     CDS             15043..16029
                     /locus_tag="NBI42_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1777738.1"
                     /translation="MFMIRSILHRLIQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAHVSAEQINATEEKLGLKDSLVSQWWQWMKQLFQLNLGRSFETKEPVTQSLFEYAPP
                     TLLISFSTLVLSLAISIPLGVLSAKHYHKLTDKVIRTVSTLSISLPAFFIGIILLFIF
                     TNLLNIDSALWNQFVLPVFTLSLGMCAYIIRLIRSNLLSLYQSNIVKSSRLRGMSERY
                     ILMHDLLKPTLLPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPIIQ
                     GCVLFIGFFVVLINTIADVLTLLFDPKQRLHFKNQKNNSNVSSSLESGDQRA"
     gene            16022..16852
                     /locus_tag="NBI42_001316"
     CDS             16022..16852
                     /locus_tag="NBI42_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1777739.1"
                     /translation="MLRSLTKGNFTFYIFIFYLIIMIILQFFVNSADATKVNLSQTFE
                     PISWTHLLGTDDYGRDLFTRVIVGARSTLFITILTLIAIVIIGVTLGLLAGYKKGWIE
                     RIILRIVDIGLSIPEFIIMIALASFFQPSIWNLVIAITIIKWMNYTRLTRSIVNSELT
                     KPYIKMAKLFHVPTRVILLRHLTPKIMPSIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQLGRDFISSHPIMLIAPASVIATTILIFNLTGDALRDKLLKQRGEYYESH
                     "
     gene            16839..17612
                     /locus_tag="NBI42_001317"
     CDS             16839..17612
                     /locus_tag="NBI42_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1777740.1"
                     /translation="MSLIDIQNLIIKNQKGKSLINGINLKIYQQKVNALIGESGAGKS
                     LTVKALLDFLPSDLNCQYDHYYFNNKNVNNMQQLYGHTVGYISQNYAESFNEHSKLGK
                     QLIAIYRTHFKVSKEAALSQIGKALSWVNLSSEEILKKYSFQLSGGQLERVYIASVLM
                     LEPELIIADEPVASLDVINGYKIMDLLQHIVKEHGQTLFIITHNLSHVLKYCDYINVI
                     KDGKIIEHGDIKHFKCDTLQPYTEFLIKYRTQLKRDHND"
     gene            17605..>18302
                     /locus_tag="NBI42_001318"
     CDS             17605..>18302
                     /locus_tag="NBI42_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1777741.1"
                     /translation="MIELKHVTFGYNKRQVILEDINIKIHDGENVGILGESGSGKSTL
                     AALLLGLLKPMNGRIYMSDDAVLPIFQHALTSFNPDWTIEDSLKEALKYYRRLKADDN
                     QRELLLQHLSNYDLDSQLLSNYPNEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVISILKEQTIANLNHLVVISHDLSVLQRLVNRIIVIKDGAIVDDFETVELFNS
                     DRHTYTKELTQVFT"
BASE COUNT         6076 a         2956 c         3035 g         6235 t
ORIGIN      
        1 cagctgttaa atgttgtacc ataaataata attctccttt aatatgttta taggttgtca
       61 aacgtataaa agccgtttgg tttattaact aagcgttctg ctgcttgtat tgcaccattt
      121 gcaaaaatat cttttgattg tgcacgatgc gtgatttgaa tcgtttcatc agtgccggca
      181 aatagaactt catgttcacc gacaatcgta cctccacgaa tagaatgaat accaatatct
      241 tgtggctggc gtttttcatt taattcatgt ctatcataaa caggagttac attttctttc
      301 aaagatacaa tcacgtcata taatttttct aacgtaccac ttggtgcatc tactttttta
      361 ttatgatgtg cctcagttaa ttcgatgtcg aaatcatcaa gtaggggaac agcagctgct
      421 aaaattttag tcagtgcatg aacgccataa ctcatgttcg cgctgaaaaa tacaggcata
      481 ttttgactca attcatctaa cttattaagt agtttttctt tctcgccagt tgttgccaca
      541 actaatggca aatgaaactc ttcatctaat aaagggaaaa gcagatttgg atttgaaaaa
      601 tctattgcaa catcggcatc tttaacatca gcaatatgtt ggtattgttg atatggcgtt
      661 gttgctttcg gtgtaggttc gattaccccc acaatttcat ggcctttttc ttctgctaat
      721 ctagcaacgc gttgattcat tgcgccatag ccaattagta atattttcac tcattttcac
      781 ccgctttaaa tatgttatat gcttcacgaa gcactttagt atctgtatct tctagtgtaa
      841 ttaatggaag acgtaattca tagtttccaa atcctaaata acttgttaaa gctttaatag
      901 gaattgggtt aatatcaact gatagggctg acaacagtat gccgattggt ttaaattgat
      961 cttgaatatc taatccgctt tgttgagcat cgtataacgc ttgaaattct ttaggaatga
     1021 cattagcaat gacagaaata acgcctttac caccacgttg atagtattcg acgacgttgt
     1081 catcattgcc actatataat gcaaatgaat ttgtatcaat tcgctttttc acttcttcta
     1141 aatactcaaa atcattcgta gcatctttta aagctacaat agaaggatgt tgacttaata
     1201 tttctacagt ttctggttca attgtcatgt tcgttcttga aggaacattg tacagcacga
     1261 ctggtaattt cacagcatct gcaatcgctt caaaatgttt tactaaacca cgttgattcg
     1321 ttttgttgta gtagggcgta attaacataa ttgcatcagc ccctaaggct ttagcttgga
     1381 ttgaagcttg gattgacttt tcagtatcat tagttccagt tccagctatg acaggaacac
     1441 gtttatctac aagatcaata actgttttta gaatgagttc tttttcatct gttgttaaag
     1501 tagggctctc agcagtagtt ccattaacga tgattgcttg ggtattattt tctagtaaaa
     1561 aattaacgtg tgctttcaaa gcttcaagat taactttgtt atttgtaaaa ggggttgtaa
     1621 gtgcaacgcc aacaccctca aataaatgtg tcattttaat tcgctccttt taaacgcata
     1681 acttgttcca atacttgtac agcatttaat gcagcacctt ttaataaatt gtctgatgta
     1741 caccatacat ggaaagtatt ttctaatgaa tcatctctac gtatacggcc aacaaatact
     1801 tcatctttat tagtagaatt gattgccatt ggatattcat tgttctctgg attgtctact
     1861 aaaacaacgc ggtcatcttg atcaaataat gctttaatat cttctgcagt cgcttctttt
     1921 tcaagcgtta catcaatttc aacactatga ctatcttgaa caggaacacg tgcacatgtt
     1981 gctgttactt ttaagtctgg tgcatttaaa atctttctag tttcatcaat catcttttgc
     2041 tcttcttttg tatatccatt ttctaaaaac acatcaatat gcggtaatac attattataa
     2101 attgggtgtg gatatgcttc aggtgcctta ccgttaatgc cctcagctaa atctttttta
     2161 cctttcatcc ctgaacctga taccgcctga tatgttgtat atgccactcg ttttaatcca
     2221 taggcatctt gtaatacttt tagaggtaca acagattgaa tcgtagagca gtttggattg
     2281 gcaatgatac ctcttgtaaa tgtaggttca ttgacttccg gaacgattaa atcaatatct
     2341 tctgccatac gccactgact tgaattgtct ataacgattg caccagcttt ttcaaagagt
     2401 ggggcaaagt gttcgcttgt accgccacca gcactcatta atacataatc gaaatgttca
     2461 cttgcacgag cctcagttaa ttcttgaact gtatatgttt ttccttgaaa ttcaacttct
     2521 tgccctgcag aacgtgctga tgaaaataat actaattcat caaaaggaat gtttttacga
     2581 tttaatgtct ctaacatttt tgtacctact aatcctgttg cacccacaac tgctaatttt
     2641 gtcataactt gtcactccat tttataataa tttccaattt ttagaatatt ttaacaatca
     2701 ttttaccatt aaatgttaaa tgcgtcatat agtttttcta ccgcttgttg cccattaaaa
     2761 tcatcaatga cgtatgaaat acttatttca gatgttgttg tttggtagaa agggatatta
     2821 ttttcaatta atgtcaaaaa tgcttttgat gccacacctg acatatcacg catacctgag
     2881 ccaattaatg aaattttgac ataatgctca ttgattttat aagctaatgc ttcatattga
     2941 ttctttaatg tttcaagaat cattgaaatt tgatgaaaat cactatcttt aatcgtgaag
     3001 gatagttgta gcccatccaa gttgacgatt tgtgaaatca tatcaacatt tacagcacct
     3061 tcttcaagtt ccgtaaatag ttgtgtaagt agttgattgt caggtagggg ataactaatt
     3121 gttacatgca tcatatgttt atccaaagcc acaccagtaa ctgctttttt ctctaatatt
     3181 tcttcatttg acataatcca tgttcctttc acgttcgata aagttttccc taaatataaa
     3241 gggatattat agtttttagc taattcaaca cttcttgttt caagtacacc agcacctaaa
     3301 gcgctcattt ccatcatttc ttcatatgag acgatgtcta gtcgtttagc ttttggtaaa
     3361 agtcttgggt cagtggcata cacaccatca acgtcggtat aaatttcaca aggtatttga
     3421 ttactaacag caagtgccac agcggtcgta tcagaaccac ctctgcctaa agttgttaat
     3481 tcttgatgtt cattgatgcc ttgaaatcca gcaactacta aaatatcgtt ttcttgaaag
     3541 gcttgttcaa atgtttgagg attaatttga gcaattttac tttttaaatg atggccgatg
     3601 gttttaatac ccgcttgata gccagtcatt gctttggcat tcacaccgat atcatttaat
     3661 accattgata aataagatac agtttgttgc tctccggttg tcaataataa tgccagttct
     3721 tgttgttttg gtgttttagt caatgtcgat acattcgtca ttaattgatc tgttgtgtta
     3781 cccatagcac ttacaacgac aattaattgt tcatcttgat tgacccgctc ctttaacatt
     3841 tcagcgatcc tttttatttt tgtaaaatca ctgacggatg atccgccaaa tttcaacaca
     3901 cttcttgtta ccatataatc ctcctaattg ttaggaaggg cacacaataa taaaaaaaga
     3961 acaagttcgg aatgcgaact tgctcttaat ctatatacaa gtgatgcact ccattatttt
     4021 taaataatga caaactctca gctcttaacc aaaaagtcca acaaattata actgctatta
     4081 taatcgcttc ggcatcgcac cctttcaaat ttagctgtta gcagacagta atctaaactt
     4141 tactcatgat tgatgcgcct catcaaaata ttattaaatt ggtatgtgtt cattatacat
     4201 atgacaaata tgaatgtaaa ccgataattt agattttttg gaattagcag aaaattcaaa
     4261 gtaaaaaagt gatttattta atatgattcg tgtgtgcctc tcctatatag cgttgttaaa
     4321 aatattttaa ttatgtataa accactgagg attattcagg acacaatttt tagtagcagt
     4381 taattattaa tggaatgttg agtaggggat agaaaaaatg aagtggaatg aataaaaggc
     4441 gaacacttta atacaaaatt tgcatcatag atgttcgcca aagtcttatt aatatatatt
     4501 aattattgat atgttttaat ctcagcttat tttaaaacgc cgatttcctg taagtattct
     4561 tcatatggaa tttcttttga aacgccacct tgtttattta aatcgataac acggtttgcg
     4621 attgtattga taaattcaaa gtcatatgaa gtaaagataa tagaaccttt gaatgattta
     4681 agtccatcat taacagcagt aatactttct aagtctaagt ggtttgttgg ttcatcaagt
     4741 agaagaacgt ttgcacttga taacatcatt ttacttagca tacaacgaac tttttctcca
     4801 cctgaaagta cactagcttt tttcttaact tcttctccgc taaatagcat acgacctaag
     4861 aagccacgta aaaatgtttc ggtttgttca tcttcaggag catattgtct taaccaatct
     4921 acaaggttca tattaacacc ctcaaagaat tctgagttat ctttagggaa gtaacttaat
     4981 gatgttgtta caccccattt ataagaacct tcgtctggtt ccatttcacc agctaatatt
     5041 ttaagtaatg ttgttttcgc gatttcacta tctccaatta aaattgcttt atcatttgga
     5101 ttcattgtga atgatacatt atctaatact ttttcgccgt caattgtttt agaaagattt
     5161 tgaacgatta ataagtcgtt accaatctca cgctcaggcg tgaatttaac gaaaggatat
     5221 cttcttgatg atggttgaat atcatctaat tcaattttct caagttgttt tttacgactt
     5281 gttgcttgtt tagatttaga agcgttagct gagaaacgag caataaagtc ctgtaactct
     5341 ttcatttttt cttctttttt cttgttttgt tcttgcgcca tcttttgagc taactgacta
     5401 gattgatacc aaaaatcata gttaccaaca taaactttaa ttttaccaaa gtctaaatca
     5461 gcgatatgag tacatacatt atttaagaaa tgacggtcat gtgatactac gataacagta
     5521 ttatcaaagt taattaagaa atcttctaac caactgattg ctggaatatc cagaccgtta
     5581 gtaggctcat ccagtaatag tacgtctggt tcaccgaata aactttgcgc taataatact
     5641 ttaatttttt ggttgttttc taattcagcc atttttttat catgtaaagt cggatcaata
     5701 cctaaaccag ataaaaggtt agctgcatca gcttcagcat tccaaccatt catttctgca
     5761 aattcacctt caagttcagc agcacgaatt ccgtcttcat cactgaaatc tggcttcata
     5821 taaatttcat ctttttcttt cataacttca taaagacgtt catgaccttt gattacaaca
     5881 tctagaacac gttcatcttc ataagcatag tgatcctgtt ttaatacagc tagacgttca
     5941 tttttaccta atgaaacgtg acctgtttga gaatctaatt ctccagataa tatttttaag
     6001 aatgttgatt tacctgcacc attcgcacca attaatccat aacaattacc ttctgtaaat
     6061 ttaatattta catcttcaaa tagtttacga tctccaaaac gcaaactgac atcagttact
     6121 tgtaacatgc attttctcct ttttttcatt cgatattcta acggaagaat tatatcatat
     6181 aatcgtcaca gtttcgacct catataagtt ctaatgatag aatgacgcac acatgttata
     6241 ataattaaga atacaagaat cgaaggagaa taacatggca ttagacaaag atatagtagg
     6301 ttctatagaa ttccttgaag taatagggct acaaggctca acttaccttt taaaaggacc
     6361 aaatggtgaa aacgttaaat taaaccaatc agaaatgaac gatgatgatg aattagaagt
     6421 aggtgaagag tatagtttct tcatttatcc aaaccgttca ggtgaattat ttgcaactca
     6481 aaatatgcct gatattacga aagataaata tgattttgct aaagtactta aaacggatcg
     6541 cgatggggca cgtatagatg ttggattacc tcgtgaagtg ttagtaccat gggaagattt
     6601 accaaaagtg aaatcattat ggccacaacc aggtgatcat ttacttgtta cattgcgaat
     6661 tgatcgtgag aatcatatgt atgggcgttt agcgagtgaa tctgtcgtag aaaatatgtt
     6721 tacacctgta cacgacgata atatgaaaaa tgaagtcatt gaagccaaac cataccgtgt
     6781 attacgaatt ggtagcttct tattaagcga atcaggttac aaaattttcg tacacgaatc
     6841 agaacgtaaa gctgaaccga gactaggtga atctgttcaa gttagaatta tcgggcataa
     6901 tgataaaggt gagttaaatg gttcattttt accacttgca catgaacgtt tagacgatga
     6961 cggtcaagtc atctttgatt tactagttga atatgatggt gaattaccat tctgggacaa
     7021 atcaagccct gaagcgatta aagaagtatt caatatgagt aaaggttcat tcaaacgtgc
     7081 aatcggtcac ttatataaac agaagattat taatatagaa acaggtaaaa tcactttaac
     7141 taaaaaaggt tggagtcgaa ttgactcaaa agaataagta aagcaaacac gtatcatcgg
     7201 atgcgtgttt tttattgcgc atggattggt caacaacttt atggtgatac tcagttacag
     7261 tctttgttgt tgaaactgtt gtggaaagac aatggtatcg tctttgttgt ggaatctgaa
     7321 agaagactta aaatatcaat aaaacaatgg ggctatatta catcggactg atggagaagt
     7381 ttttatcttt tcatcagtct atttttaatt cccaatttta tttatcaata gatatgtata
     7441 ccttgtcaga atttttatga ggcattttaa ttttacgaag ataaaccatt tttttaataa
     7501 gcaaaaataa tatttacgat ttcttaacaa acatttatgc aacatttaca gtttctaaaa
     7561 atgagattaa taattcaagg ttaagataaa ggtgtaatca atacaaatac tatttgttgt
     7621 tcatacaggg aggatatttc aatgaaaaaa tggcaatttg ttggtactac agctttaggt
     7681 gcaacactat tattgggcgc ttgtggtggc ggtaatggtg gcagtggcaa tagtgattta
     7741 aaaggggaag caaaaggtga tggctcatca acagtagcac caattattga aaaattaaat
     7801 gaaaaatggg ctcaagatca ctcagatgct aaaatctcag caggacaagc tggtactggt
     7861 gctggtttcc aaaaattcat tgcaggagat atcgactttg ctgatgcttc tagaccaatt
     7921 aaagatgaag agaagcaaaa attacaagat aagaatatca aatacaaaga attcaaaatt
     7981 gcgcaagatg gtgtaacggt tgctgtaaat aaagaaaatg attttgtaga tgaattagac
     8041 aaacagcaac ttaaagcaat ttattctgga aaagctaaaa catggaaaga tgttaatagt
     8101 aaatggccag ataaaaaaat aaatgctgta tcaccaaact caagtcatgg tacttatgac
     8161 ttctttgaaa atgaagtaat gaataaagaa gatattaaag cagaaaaaaa tgctgataca
     8221 aatgctatcg tttcttctgt aacgaaaaac aaagagggaa tcggatactt tggatataac
     8281 ttctacgtac aaaataaaga taaattaaaa gaagttaaaa tcaaagatga aaatggtaaa
     8341 gcaacagagc ctacgaaaaa aacaattcaa gataactctt atgcattaag tagaccatta
     8401 ttcatttatg taaatgaaaa agcattgaaa gataataaag taatgtcaga atttatcaaa
     8461 ttcgtcttag aagataaagg taaagcagct gaagaagctg gatatgtagc agcaccagag
     8521 aaaacataca aatcacaatt agatgattta aaagcattta ttgataaaaa tcaaaaatca
     8581 gacgacaaga aatctgatga taaaaagtct gaagacaaaa aataataaga cgcaatttca
     8641 aatgtgtctt gaaacatgat tttgatggtg aatcattatt tagagtacaa agcttgattt
     8701 atcgagacgc tgattttgac attcagttag tctacaagct tatcaactta aaatagtggt
     8761 tcatcattat tttacaaatt taattatttt gggagtaata gaaagaggtt tgattatgac
     8821 ttcatctact aatgttaaag ctttaattga aaaaaataat aataaaaaag ggaagcataa
     8881 tgacaaaatt ataccagtta ttttagccgc aatttcagcg atttccattt taacaacact
     8941 aggtatatta atcacattgc ttttagaaac catcactttt ttcaccagaa ttccaataac
     9001 tgaatttcta ttttctacta cttggaatcc taccggttct gaccctaagt ttggtatctg
     9061 ggcattgata atagggactt tgaaaatcac agttattgcg actatatttg cagttccagt
     9121 cgggcttggt gcagcaattt atttaagtga atatgcaagt gaccgtgcac gacgcataat
     9181 taaaccgata ttagaaattt tagcaggaat accaacaatt gtgtttggtt tctttgcatt
     9241 gacctttgtt acaccagtat taagatcttt cataccaggt cttggagagt ttaatgctat
     9301 aagtcccggc ttagttgtcg gtattatgat tgtccctctc atcacaagtt tgagtgagga
     9361 tgcaatggca tctgtaccaa ataaaattcg agaaggtgcc tatggacttg gagcaactaa
     9421 attagaagta gcaactaaag tcgtacttcc tgcagcaaca tcaggtattg tagcttcaat
     9481 cgttctcgcg atttcaagag caattggaga aacgatgatt gtatcattag cggcaggtag
     9541 ttcgccaaca gcttcattaa gtttaacaag ttcgattcaa acaatgactg gatatattgt
     9601 tgagatagcg acaggtgatg caacatttgg atcaaatatt tattacagta tttatgctgt
     9661 aggattcaca ttatttattt ttaccttaat catgaattta ctttctcaat ggatttctaa
     9721 gcgttttagg gaggagtatt aatatggaaa cgacagataa taatagacaa tcactcgtcg
     9781 atcaacaact tgtccaaaag catttatcat ccagaacggt taaaaataaa gtgttcaaac
     9841 tcatattttt agcatgtaca ttattagggc ttgtcgtact tattgcgttg ttaactcaaa
     9901 cattgattaa aggggtaagt catttaaatt tacagttttt cactaacttt tcttcttcaa
     9961 caccatccat ggctggcgtt aaaggcgcgt taatcggttc actttggtta atgttaagta
    10021 tcattccatt atcaatcatc ctaggaatag gtacagctat atacttagaa gaatatgcga
    10081 aaaacaacaa atttactcag tttgttaaaa tcagtatttc caatttagct ggtgtaccat
    10141 cagttgtatt tggattatta ggttacactt tgttcgttgg tggtgcaggg attgaagcct
    10201 tgaaaatggg taacagtata ttggcagcag cgctaacaat gaccttactg atattaccaa
    10261 ttattattgt ctcaagtcag gaagcaatta gagctgtacc taactcagta cgcgaagctt
    10321 cttacggctt aggtgctaat aaatggcaaa cgataagacg tgttgtctta ccagcagcgt
    10381 tacctggtat tttaactgga ttcattttgt ctctttcaag agcactggga gaaacagcac
    10441 cacttgtgct tatcggtata ccgactatat tattggcaac acctagaagt atattggatc
    10501 aattttcagc attacctatc caaatattta cttgggcgaa aatgccacaa gaagaattcc
    10561 agaatattgc atcggcaggc attatcgttt tactagttat cttaatctta atgaatggcg
    10621 ttgcgattat tttacgtaac aaatttagta aaaaattcta atttaaacaa tcaatctcat
    10681 ttatctatta aaaagggagt tttaaatatg gcgcaaacac ttgcacaaac taaacaaata
    10741 tctcaaagtc atacgtttga taactcacaa agtcatcata caacaccaaa tgatacaaac
    10801 tcacattctg ttatatattc aacacaaaat ttagacttat ggtatggcga aaatcatgca
    10861 ttgcaaaata ttaatttaga tatttatgaa aaccaaatta cagccattat aggtccatct
    10921 ggttgcggta aatcaacata tattaaaact ttgaatcgca tggttgagtt agtaccttct
    10981 gtgaaaacag ctggtaaaat attatatcga gatcaagaca tttttgatca aaagtattca
    11041 aaggaacaat tacgtactaa tgtaggtatg gtctttcaac aacctaatcc atttccaaaa
    11101 tcaatatacg ataatattac ttacggtcca aagattcacg gtattaaaaa taaaaaagtt
    11161 cttgatgaaa tcgttgagaa atcattacgt ggcgctgcaa tttgggatga attaaaggat
    11221 aggttgcaca caaatgcata tagtttatcc ggtgggcaac aacagcgtgt ttgtatcgcg
    11281 cgttgtttag caattgaacc tgaagtcatt ttaatggatg aaccgacatc agcattagac
    11341 ccaatctcaa cattaagagt agaagagttg gttcaagaac taaaagaaaa gtatacaatt
    11401 attatggtta cacataatat gcaacaagca gctcgtgtat cagataaaac tgcatttttc
    11461 ttaaatggtt atgtcaatga atatgatgac actgataaaa ttttctctaa cccatcaaac
    11521 aagaaaacag aagattatat ttcaggaagg tttggttgat atataatggc aataattaga
    11581 caacgatatc aggagcaact tgatgattta ataaaagaat tacgtcggtt aggtgcaaat
    11641 gtctatgtga gtattgaaaa tggtataaaa tcattaagta ttgacgatag aggctttgca
    11701 cgacaaacag ttaaaaacga taaacatatc aatcaattaa attatgatat taatgagcga
    11761 gttatcatgt taattacaaa gcaacagccc attgcgagtg atttgcgtat gatgatttct
    11821 tcattaaaaa tcgcctccga tttagaaaga ataggagata atgcatcgag tattgccaat
    11881 attcgattgc gtacgaagat tacagatgat tatgtgttaa cccgtttaaa gacaatgggt
    11941 aaactagcta tgttaatgtt aaaggattta gatcaagcat ttaaaaagaa agataccgta
    12001 ttaataagag aaataattga acgagatgaa gatatcgatg acttatatag tcatattatt
    12061 aacgcaacgt atcttattga taacgatcca tttgtcgctg cacaagctca tttagcagca
    12121 agacatttag aacgtattgg tgatcatatt attaacatcg ctgaaagtgt ttatttttat
    12181 ttaacaggta cacattacga acaataactt aaagttatta ctataaaatc ccttacgata
    12241 aatatatatt tctattattc ataaaccctc aaaaaaacca agattctcac aattagtaat
    12301 gtgaaaatct tggtttatat tgttctacta taaattgtct cgcatcttag ttatttgctt
    12361 tctcaatttc atctgttaat ttttcaactt catctactaa atcagaaata tattgtattg
    12421 tagtttttag tggctgatct gtagtaatgt ctacacccgc aatttttgca agttcgacag
    12481 gtgatacact tccacctttt ttcaatgttt ctaaccaagc atcaatagct ggttgacctt
    12541 catttttaat cttttgagaa acgacagtgc cgattgttaa gccggcagaa tacgtatacg
    12601 aatataatcc catatagtaa tgaggttggc gcatccacgt taattcagca ccttctgtca
    12661 tttctactgc atctccgaaa aattgtttat atacacttat cataatttca tttaaagtag
    12721 gcgcatttaa tgactcccct tgatctactt tacgatatac ttcacgctga tatgcagctt
    12781 ctaataaatg ggttaccatg ttgtgataat atgttctaga taaaatagag ccaataaccc
    12841 agcgtttaaa tcttggatta tcacttgtgt taaacaagta attggccatt aacatttcat
    12901 tcattgtaga aggcgcttca acaaagtaca ttgatgcttc tgattcaagg tatggttgat
    12961 gtttttgagc taatgtaaaa tgacctgcgt gccctaattc atgtgctaat acaaatgctt
    13021 cggccatttt tccagtccaa gaaataaaaa cataagaatg cgtaaagtac ggactcgcac
    13081 aaaatgcacc agtatcttta cctttatttt gtgcaaaatc aatccatcgc tgatcgtatg
    13141 cttcacgtaa catgtttgta tagtcatcac ctaaaacact taacgcaccg aaaatatatt
    13201 tttttgaatc ttcaatcgaa atttctggtt cataatcagg gtctacagaa attttcaaat
    13261 ctttaaaacg catgttatct aatccgtgaa tacgttgtaa aagtttagca tatttctgca
    13321 taactggtgc caagtcacgc ataatcatat cgatttgacg gtcaaacata tcacgcgtta
    13381 cttcttgact atgtaataaa taatcgatga ccgattcaaa tccacgtaaa tcagcttcaa
    13441 ttttttcttg ttgtacttgc atattatatg ttgccgcggt agtatgctga tattttcgaa
    13501 tcccatcgct aaacgatttg aaacttttac gtctaaactc aggatcttta ttatcttcat
    13561 aatcattttc aaacgtagca tagtcgacag ggtacgttgt accatcatgt tcaaatgaat
    13621 cgaatgtaat atctagcatt tttgtcgtgc cgtataaatc gtaaggactg tttagcgtag
    13681 gtgataaagt tgctaataat tgttctaccg acgcagataa ttggaatggc ttttgtttta
    13741 ttaactgttt aatatagtgt tgatatggac atgattcttc taattgttga agtatttctt
    13801 ctggtaattc aagtatttct gactctacaa atgataatcg gctaacaatt tttccgtatg
    13861 tagtagataa tttagcgctc aatacttgtg cctcaatgtt actagtatct acacttaaac
    13921 gtagttctgc ataattactt aagcaatcta aggcaattaa aatattttct aattcagcta
    13981 aagcagtatt aatttgttcg attgaattta atgttgttgc atatgtatgg tgaaattgat
    14041 ttgcttgttg tagtaaggat tctatacttt catagtattg ttgatcatct ttaaataaat
    14101 ctgttaaatc ccatgtttct gaaactggaa catcttctct taaaggtaaa ccttgagaca
    14161 tataatcact cctttggtat aatactttca ttatacaaaa ttttaatgcc tgaacgataa
    14221 tgttacgatt tccaagaagc aacgcattat gacttttttc tatagggaga agagatgcta
    14281 cttaaaactg actgccattg tttaagtttt taaaatttta taataaaatg tatacaagga
    14341 aagtattgaa ataaaaggag aatggttatg acattattcg atatgcctaa ttatttatgg
    14401 atcacaactt taataatgat tttattaaca gtattctgtt gcttagtttt aaataaatgg
    14461 tttgtttcag cagtaataac atttgtagtt ttaggcgtac ttgccttttt tattccaaat
    14521 tttcaagaaa taaaatatca accattgtta ggatatgctg catttttagc catcatgagt
    14581 ttaataataa gttttctatt atggtatttc actagaaatt ggcgtaaaga tagaaaagct
    14641 cgtaaattag aaaagcaaat tgaaaaatat ggctatgagg gcgctgaact tcgccgtaaa
    14701 gataagaagt aaattttaac aactatatgc acaaaataaa aaacttcccc tgaaaattgt
    14761 tttaatggaa gtttatgaac ggatttattt attaaaagtg ctatagttgt ttttgttttg
    14821 ttcaaattta agttgtaaat tcatattaaa ttgtaggtta tcaactctga ataaaaatac
    14881 tcattttaaa agcgtttatt gtcaaaataa ataaatttac ttcaatttct ttttgtataa
    14941 tactataatg aatcgtggtt ctttatttgt aagttatgaa aatgtgtgta attctcatac
    15001 aaattcaaaa tatgattaac ttacacaagg agggggactt ttatgttcat gataaggtct
    15061 attttgcata gactgataca aatgatagtt gttttatttg ttatttcaac actaactttc
    15121 attttgatga aattatctcc aggaaatcca gttgataaaa tacttcatct tgatgtggca
    15181 catgtgtcgg cagaacaaat aaatgcaact gaagaaaaat tgggtttaaa ggattcactc
    15241 gtatcgcagt ggtggcaatg gatgaaacaa ttgtttcaat taaatttagg acgtagtttc
    15301 gaaactaaag aaccggttac acagtcacta tttgagtatg caccaccaac tttgctcata
    15361 tctttttcaa cattagtttt gtcattagcg atatcgatac cactaggtgt actttcagca
    15421 aaacactatc acaaactgac tgataaagtg attagaactg tatcaacttt gtcaattagc
    15481 ttgcccgcgt ttttcattgg tattatttta ttattcattt tcaccaattt attaaatatt
    15541 gatagtgcct tatggaatca gtttgtatta cctgttttta cattgtcatt aggtatgtgt
    15601 gcatatatta ttcgattaat tcgttcaaat ttactttcgc tatatcaaag caatatcgtt
    15661 aaatcatcac gattacgcgg tatgagcgaa cgctatattt tgatgcatga tttattgaaa
    15721 ccaacgcttt tacctattat tccattgtta ggtatttctc taggcagtct tattggcggt
    15781 acagttgtta ttgaaaattt atttgatata cctggcattg gttacttatt gatggatagt
    15841 attaaatctc gggattatcc aatcatacaa gggtgtgtat tgtttattgg attttttgtg
    15901 gtgttaatca atacaattgc tgacgtactc actctattgt ttgatccaaa acaacgttta
    15961 cattttaaaa atcaaaaaaa taattcgaac gtatcatcaa gtttagaaag tggtgatcaa
    16021 cgtgcttaga agtttgacta aaggtaattt tacattttat atatttatct tttatttaat
    16081 aattatgatt attttgcaat tctttgtgaa tagtgctgat gcaacaaaag ttaacttatc
    16141 gcaaacgttt gaaccaataa gttggaccca tttactagga acagatgact atggtcgtga
    16201 tttatttaca agggtcattg ttggtgcacg atcaacatta ttcatcacaa tcttaacttt
    16261 aattgctatt gttattattg gggtaacact tggattactt gcagggtata aaaaaggatg
    16321 gatagagcgc attatattac gcattgttga tatcggtcta agtataccgg aatttattat
    16381 catgatagca ttagcaagtt tttttcagcc aagtatttgg aatttagtta ttgcaattac
    16441 aattataaaa tggatgaatt atacaagatt aacaagaagt attgttaata gcgagcttac
    16501 taaaccttac attaaaatgg caaaattatt tcatgtacca actagggtaa tattgttacg
    16561 ccacttaaca cctaagataa tgccgtcaat tatcgttttg atggtggtcg atttcgggaa
    16621 aatcatttta tacatcagct cattatcatt cataggacta ggtgcacaac caccgacacc
    16681 agagtggggc gctatgttgc aacttggcag agattttatt tcgtcacatc caattatgct
    16741 cattgcgcct gcttctgtca ttgctacaac aattttaatt tttaatctaa ctggcgatgc
    16801 attaagagat aaattgttga aacaacgggg tgaatattat gagtctcatt gatatacaaa
    16861 atttaattat caagaatcaa aaaggtaaat ctttaataaa tggcatcaat ctgaaaattt
    16921 atcaacaaaa ggttaacgct ttaattggag aaagtggcgc aggtaagagt ttgacggtaa
    16981 aagctttgct agatttttta ccatctgatt taaattgtca gtatgatcat tattacttca
    17041 ataataaaaa cgtaaacaac atgcagcaat tatatggtca cactgttgga tatatttctc
    17101 aaaattatgc tgaaagtttt aatgagcact cgaaattagg aaaacaattg attgcaattt
    17161 atcgtactca ttttaaagtt agtaaagaag cagcattatc tcaaattggt aaagctttgt
    17221 cgtgggttaa cttaagtagc gaagaaatat tgaagaaata cagttttcaa ctttctggag
    17281 gtcaacttga acgcgtatat atcgcaagtg ttttaatgtt agaacctgag ttaattattg
    17341 cagatgaacc agtagcatca ttagatgtta ttaacggcta taaaattatg gatttacttc
    17401 aacacatcgt aaaagaacac ggtcaaacat tatttataat cactcataac ttaagccacg
    17461 ttttaaaata ttgtgactat attaatgtta taaaagatgg caaaattatt gagcatggtg
    17521 acataaagca ctttaagtgc gatacattac aaccttatac cgagttttta attaaatatc
    17581 gaacacaatt aaagagggat cataatgatt gagttaaaac atgtgacatt tggctataat
    17641 aaaagacaag taatattaga agacatcaat attaaaatac atgatggcga aaatgttggc
    17701 attttaggag aaagtggtag tggtaaaagt acattggcgg cattgttatt agggttactt
    17761 aagccgatga atgggagaat ttatatgagt gatgatgctg tattaccaat ttttcaacat
    17821 gcattaacaa gttttaatcc tgattggaca attgaagact cattaaaaga agcactaaaa
    17881 tattatcgta ggttaaaagc cgatgacaat caacgggaat tattattaca acatttatca
    17941 aactatgatt tagattcaca actgttatct aactatccta atgaagtaag tggtggacaa
    18001 ttacaaagat ttaatgttat gcgttcatta ttagcgcaac ctcgtgtttt aatatgtgat
    18061 gaaatcacgt caaatctaga tgttatagct gagcaaaacg taataagcat tttaaaggag
    18121 caaaccattg cgaatttaaa tcatttagtt gtcatatcgc atgacttatc ggtactacaa
    18181 cgtttagtta atcgaattat tgtcattaag gatggtgcaa tcgttgatga ttttgaaacg
    18241 gttgaattat tcaattcaga tagacatact tatacaaaag aattaacaca agtatttaca
    18301 tt
//
LOCUS       DAHHRR010000043        17946 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.043, whole genome shotgun sequence.
ACCESSION   DAHHRR010000043 DAHHRR010000000
VERSION     DAHHRR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17946
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.043"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            179..1147
                     /gene="pfkA"
                     /locus_tag="NBI42_001319"
     CDS             179..1147
                     /gene="pfkA"
                     /locus_tag="NBI42_001319"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCD1777742.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            1169..2926
                     /gene="pyk"
                     /locus_tag="NBI42_001320"
     CDS             1169..2926
                     /gene="pyk"
                     /locus_tag="NBI42_001320"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCD1777743.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVTNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
     gene            complement(3273..4634)
                     /locus_tag="NBI42_001321"
     CDS             complement(3273..4634)
                     /locus_tag="NBI42_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1777744.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFPKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLGFMYLRYKRIAAK
                     SNK"
     gene            4982..6103
                     /locus_tag="NBI42_001322"
     CDS             4982..6103
                     /locus_tag="NBI42_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HCD1777745.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            6152..7420
                     /gene="icd"
                     /locus_tag="NBI42_001323"
     CDS             6152..7420
                     /gene="icd"
                     /locus_tag="NBI42_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCD1777746.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALTEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            7809..8513
                     /locus_tag="NBI42_001324"
     CDS             7809..8513
                     /locus_tag="NBI42_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1777747.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVEN
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            8513..10174
                     /locus_tag="NBI42_001325"
     CDS             8513..10174
                     /locus_tag="NBI42_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCD1777748.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKEIVKQQNIDLLITKNDEKVFSSGKLKAIPNSDSFIQKKDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
     gene            10674..12161
                     /locus_tag="NBI42_001326"
     CDS             10674..12161
                     /locus_tag="NBI42_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777749.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTFGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMLSTSLPYGVIFSQVIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFANVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILV
                     KEFRKYKSINVNFIKVASLVLAMIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
     gene            12454..15084
                     /gene="polA"
                     /locus_tag="NBI42_001327"
     CDS             12454..15084
                     /gene="polA"
                     /locus_tag="NBI42_001327"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCD1777750.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNTKKVVYDAKKTYVASHRLEIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIYTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            15100..15972
                     /gene="mutM"
                     /locus_tag="NBI42_001328"
     CDS             15100..15972
                     /gene="mutM"
                     /locus_tag="NBI42_001328"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCD1777751.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKDQGKETII
                     KGIELDTFKTLSEGYTITNVKRRSKYIVFQLDNKREKRILISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKIKDLTYQQQ
                     EMVFYYVREVLEEGIKHGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            15988..16611
                     /locus_tag="NBI42_001329"
     CDS             15988..16611
                     /locus_tag="NBI42_001329"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCD1777752.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLKEEGYIEKPNYGEED"
     gene            16781..17806
                     /gene="gap"
                     /locus_tag="NBI42_001330"
     CDS             16781..17806
                     /gene="gap"
                     /locus_tag="NBI42_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD1777753.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDDQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPHSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
BASE COUNT         6481 a         2428 c         3353 g         5684 t
ORIGIN      
        1 ttttaactaa gattagtagt caaaattgtg gctactaatc tttttttaat taagttaaaa
       61 taaaattcaa tatttaaaac gtttacatca attcaataca ttagttttga tggaatgaca
      121 tatcaatttg tggtaattta gagttaaaga taaatcagtt atagaaaggt atgtcgtcat
      181 gaagaaaatt gcagttttaa ctagtggtgg agattcacct ggaatgaatg ctgccgtaag
      241 agcagttgtt cgtacagcaa tttacaatga aattgaagtt tatggtgtgt atcatggtta
      301 ccaaggattg ttaaatgatg atattcataa acttgaatta ggatcagttg gggatacgat
      361 tcagcgtgga ggtacattct tgtattcagc aagatgtcca gagtttaagg agcaagaagt
      421 acgtaaagtt gcaatcgaaa acttacgtaa aagagggatt gagggccttg tagttattgg
      481 tggtgacggt agttatcgcg gtgcacaacg catcagtgag gaatgcaaag aaattcaaac
      541 tatcggtatt cctggtacga ttgacaatga tatcaatggt actgatttta caattggatt
      601 tgacacagca ttaaatacga ttattggctt agtcgacaaa attagagata ctgcgtcaag
      661 tcacgcacga acatttatca ttgaagcaat gggccgtgat tgtggagatc tagcattatg
      721 ggctggatta tcagttggtg ctgagacaat tgtagttcca gaagtgaaaa cagatattaa
      781 agaaatagct gataaaattg aacaaggtat taaacgtggt aagaaacact caatcgttct
      841 tgtagcagaa ggttgtatga ctgcacaaga ttgtcaaaaa gaattatcac aatacatcaa
      901 tgttgataat agagtgtctg tgttaggtca cgttcaacgt ggtggtagcc caacaggtgc
      961 ggatagagtt ttagcatcac gtttaggtgg atatgcggta gacttattaa tgcaaggtga
     1021 aacagctaag ggtgttggaa ttaagaacaa taaaattgta gcaacatctt ttgatgaaat
     1081 ttttgatggt aaagatcata aatttgatta tagtctatat gaacttgcta acaagttatc
     1141 tatataagat ttcaggagga attataaaat gagaaaaact aaaattgtat gtacaattgg
     1201 accagcttca gaatcagaag aaatgattga gaaattaatc aatgctggta tgaacgttgc
     1261 acgattaaac ttttcacatg gtagtcatga agagcataaa ggtagaattg atacaattcg
     1321 taaagtagct aaaagattag acaaaattgt agcaatttta ttagatacga aaggtccaga
     1381 aattcgtacg cataatatga aagacggtat cattgaactt gaacgtggta acgaagttat
     1441 tgttagcatg aatgaagttg aaggaacacc tgaaaagttc tcagtaacat atgaaaactt
     1501 aattaacgat gttcaagtag gttcatacat tttacttgat gatggcttaa ttgaattaca
     1561 agttaaagat attgatcatg ctaaaaaaga agttaaatgt gatattttaa actcaggtga
     1621 gcttaaaaac aaaaaaggtg ttaacttacc tggtgtaaga gtaagtttac ctggtattac
     1681 agaaaaagat gctgaagata tccgtttcgg tattaaagaa aatgttgact tcattgcagc
     1741 aagtttcgta cgtcgtccta gtgatgtttt agaaattcgt gaaattttag aagaacaaaa
     1801 agctaacata tcagtatttc ctaaaattga aaaccaagaa ggtattgata atattgcgga
     1861 aattcttgaa gtgtctgatg gtttaatggt tgcacgtggt gacatgggtg ttgaaattcc
     1921 acctgaaaaa gtaccaatgg ttcaaaaaga tttaatcaga caatgtaaca aattaggtaa
     1981 accagttatt acagctacac aaatgttaga ttctatgcaa cgtaacccac gtgctacccg
     2041 tgcagaagct agtgacgttg ccaacgcaat ctatgatggt acagatgcag taatgttatc
     2101 tggtgaaact gctgctggtt tatatcctga agaagctgtt aaaacaatga gaaatattgc
     2161 tgtatcagct gaagcagccc aagattacaa aaagttattg tcagatcgta ctaaattagt
     2221 tgaaacttca ttagtgaatg ctatcggtat ttcggttgca catacagctt taaacttaaa
     2281 tgttaaagca attgtagctg ctactgaaag tggttcaacg gcacgtacta tctctaaata
     2341 tcgtccgcat tcagacatta ttgcagtgac tccaagtgaa gaaactgcac gtcaatgttc
     2401 aattgtttgg ggagttcaac cagtagttaa aaaaggacgt aagagtacag atgcattgtt
     2461 aaacaatgca gttgcaacag ctgttgaaac tggtagagta actaatggtg atttaatcat
     2521 tattactgct ggtgtaccaa ctggtgaaac tggaactact aatatgatga aaatccacct
     2581 agttggtgac gaaattgcta atggtcaagg tattggacgt ggatcagttg ttggtactac
     2641 gttagttgct gaaactgtta aagatttaga aggtaaagat ctatctgaca aagttatcgt
     2701 tactaactca atcgatgaaa cgtttgtacc ttatgtagaa aaagctttag gcttaattac
     2761 agaagaaaat ggtattacat caccaagtgc aattgttggt ttagaaaaag gtattccaac
     2821 agttgtaggt gtagaaaaag ctgttaaaaa tataagcaat aacatgttag ttacgattga
     2881 tgctgctcaa ggtaaaatct ttgaaggata tgcaaacgta ctataattta taaaaaacgt
     2941 ctttccattt attaacaatg gaaaggcgtt ttttggttca tctggtattt tatgacgtaa
     3001 ttaataggtt atttgataat gatagtgtat gaatggcaat ctatataaat gtttatatct
     3061 tttatacatg tacattatca cctttaaact ttcactcata ttactttgga aagttattat
     3121 aaaatagaag tatggatgta tttctgaaat gatacattat taaatagatg agaaagtaaa
     3181 agcattgagc caagtacgca atttaatatt ataagttgca tataaaaacc cctgttgaac
     3241 tttaatatat tcaacagggg taaaacttgt tgttatttat ttgatttagc agcgatacgt
     3301 ttatatctta agtacataaa tcctaatagt ataaaccaaa taggaataaa ataaattgca
     3361 cgtcttgtat caacattaat aaataataac ccgaacacaa aaatgaagaa tacaaatatt
     3421 aagtagccca tatatttacc acctaatagt ttgtacgtag catttttatg tagatctggg
     3481 tttttacgac tataattgat atatgcaatg ataatcagac cccatacaac taaaaataac
     3541 actgtagaga tggtagtcac atacgtaaat acttttgtcg catctgggaa aatatagttt
     3601 agtaatgctg ccacaagtaa taatgctgat gaagcaaaga ttgcaacatg tggaacgcca
     3661 tatttattcg ttttagaaaa gttcggaggt gcttgttgtt gacttgataa accgaaaagc
     3721 atacggctat ttgagaatat accactgtta catgatgaag cagcagcggt taatactaca
     3781 aaattaatca agcccgcagc aaacggaatt ccgatcaatg cgaataattt tacgaatgga
     3841 ctgttatcag gatcaacttg ctgccaaggg ataatagaca tgataaccgc taacgcacca
     3901 acgtaaaata ttaaaattct aatgggtaca ctgttaattg cttttggaat tgttttaact
     3961 ggatcttttg tttccccagc tgtaacacca atcatttcaa ttcctacaaa tgagaataat
     4021 gccatttgga aagacataaa gaatccagaa gcacctttag ggaatatgcc gtgttcatat
     4081 agatttgtga aactggcatg cccgaattga gttttaaaag caaatagaat catgacgaaa
     4141 ccaactacta ttaaaccaat aattgtcgct attttaatga tagagaacca aaattctaat
     4201 tctccgaaaa gtcttgcgct aagtaggttg aatgacatta ataacagtac acaaaatagt
     4261 gcacttatcc agtttggaat ttctgggaac caaaagctaa catattttgc cacagccgtt
     4321 acttcagcca tacctgtaat aatccaacag aaccagtatg tccatccggt aacaaatcct
     4381 gcaaaaggcc caatatatgt attggttaca tctgcgaaag atttaaattc agtattctgt
     4441 ataatgattt ctcctaaacc tcgcataaac ataaataaca taaatcctat aatgatgtat
     4501 gttaatagaa ttgaagggcc ggttaatgca atcgtttgac cagcacctaa gaataagcct
     4561 gtaccaattg caccgccaat tgcaattaat tgtatgtggc gattgctcag ttccctttgt
     4621 aatttttcag ccataataca tctcccttaa atatagatat gtttattatg cacttatatt
     4681 gagatatata caattatttt cggtaaaaat gtgtaaaatt ccatgttaat atactttggt
     4741 ttttataatc atatataata accaattgaa aatttaattc tattgtaaaa ttcatggatt
     4801 attcacatct tgaaaaagct ttaatggtgc tatttgtggc tattctgtga catttacata
     4861 gatttacaaa ataattgttg cacatataat gccagttttt atatttcaca aacgaaatgc
     4921 gtttagctat aatattagtt gaaagccatt tcataaagaa acagtaaagg ggaaatttat
     4981 catggcagaa ttacaaagag gtttagaagg ggttatcgca gcggagacta aaataagttc
     5041 aattattgaa agtcaattga cttatgccgg ctatgatatt gatgatctag ctgaaaatgc
     5101 gcaatttgaa gaagttattt tcctattatg gaactataga ttgccaaatg aagaagagct
     5161 tgctcatcta aaagggaaat taaatcaata catgacatta aatcctcgtg tgtatacaca
     5221 ttttgaggag tatgttacag atcacgtgca tccaatgaca gcattacgta cgtcgttatc
     5281 atatattgca catttcgatc ctgatgctga aaatgaatca gatgaaaatc gttatgaaag
     5341 agcaatgcgt atacaggcta aagtagcatc attagttaca gcgtttgctc gagtaagaca
     5401 agataaagaa ccacttaagc ctaatcctga cttaagttat gcggcaaact tcctatatat
     5461 gttacgtggg gaattaccaa cagatataga agtagaagcc ttcaataaag cacttatttt
     5521 acacgctgat catgagttga acgcatctgc atttacagca cgttgtgcgg tatcatcatt
     5581 gtcagatatg tactcaggta ttgtagcagc tgtaggttct ctgaaagggc cattacatgg
     5641 tggtgcaaac gaacaagtta tgacgatgtt atctgagatt gggtcaattg aaaatgttga
     5701 tgcttactta gatgaaaaat ttgctaataa agataaagta atgggcttcg gtcatcgtgt
     5761 atataaagat ggtgatccta gagcgaaata tttaagagaa atgagccgtc aaattacgaa
     5821 agacgctggt cgtgaagaat tatttgaaat gtcagtgaaa atggaaaaac gtatggcaga
     5881 agaaaaagga ttaattccta atgttgattt ttatagtgcg agtgtttatc actgtatgga
     5941 aatacctcat gacttattca cgccaatctt tgctgtaagt cgttctgcag gatggattgc
     6001 tcatatttta gaacaatata aagataatag aattatgcgt cctagagcga aatatattgg
     6061 cgaaacgaat cgtaagtata tcccgcttga agaaagaaaa taatcaatgc aaattaaaaa
     6121 tgaagatgta aaatttggag gtaaaataac tatgactgca gaaaaaatta ctcaaggaac
     6181 tgaaggatta aacgtaccta atgaaccaat tatcccattt attatcggtg atggaattgg
     6241 accggatatt tggaaggcag caagccgagt tatagatgct gctgttgaga aagcctataa
     6301 tggcgaaaaa cgcattgaat ggaaagaagt gttagctggc caaaaagcat ttgatacaac
     6361 tggcgaatgg ttacctcaag aaacacttga tacaattaaa gaatatttaa ttgctgttaa
     6421 gggaccttta acaacaccaa ttggtggtgg tattagatca ttaaatgtag ctttacgcca
     6481 agaattagat ttatttactt gtttaagacc ggtacgttgg tttaaaggag taccatcacc
     6541 tgttaaacgt ccacaagatg ttgatatggt tattttccgt gaaaatactg aagacattta
     6601 tgctggtatt gaatttaaag aaggtacaac agaagttaaa aaagtaattg acttcttaca
     6661 aaacgaaatg ggtgcgacaa acattcgatt cccagaaaca tcaggtattg gtattaaacc
     6721 agtttctaaa gaaggtactg agcgattagt tagagcagct attcaatatg ctatcgataa
     6781 taatcgtaaa tcagttactt tagttcataa aggtaatatt atgaaattta cagaaggctc
     6841 atttaagcag tggggttacg atttagcatt aactgaattt ggtgatcaag tattcacttg
     6901 gcaacaatat gacgaaattg ttgaaaatga aggcagagat gctgctaatg ctgcacaaga
     6961 aaaagctgaa aaagaaggca agattatcat taaagattct attgctgaca ttttcttaca
     7021 acaaatttta actcgtccag ctgagcatga tgttgtagca actatgaact tgaatggtga
     7081 ctatatttca gatgctttag ctgcacaagt tggtggtatt ggtattgcgc caggtgcaaa
     7141 cattaattat gaaacaggtc atgctatttt tgaagcaaca catggtacag ctccaaaata
     7201 tgcaggttta aataaagtga atccatcttc agtaatttta agttctgtat taatgttaga
     7261 acatttagga tggcaagaag cggcagataa gattacagat tcaattgaag atacaattgc
     7321 ttcaaaagtt gttacttatg actttgcccg tttaatggat ggtgctgaag aagtttctac
     7381 atcagcattt gcagatgaat tgattaaaaa tttaaaataa gcaaaataaa cttaggtgag
     7441 tctagcaact ttaattgcta ggttcgcctt tttaaatgaa ttgaagttgg caacgggaca
     7501 gaaataatta agagtcacta atgattgatt atgtagtgtt tcttacacat taaccaaagc
     7561 gaatgtgtac ataagcttag ggataaatga gtaaaactca tgcataggta gtgggataga
     7621 aatgatattt tcacaaattt atttcgttgt cccagagtaa gtcatgcaaa ttatagtagg
     7681 aaatataaga agtattttat ttatgtgtaa agtaatgtaa aaaatgtgta aagataatgt
     7741 gtaggatgtt atttttataa atagttcggt tataatgtaa atataattat aagttaggga
     7801 ggcataccat gtcgcaaaaa gtgttggtag tagatgacga acattcaatc gtaacattac
     7861 ttaaatataa cttagaaaca gctggttatg aagttgttgt cgcatttgat ggtgatgagg
     7921 ctttagaaaa ggtagaaaat gaacagccag atttaattat tttagatgtt atgctaccta
     7981 aaaaagatgg cattgacgta tgtaagactg taagaacaaa taaaaattta gtccctatat
     8041 taatgttaac ggcgaaaaat gatgaatttg atcgggtatt aggtttagaa ttaggtgccg
     8101 atgactatat gactaagcct ttttcaccta gagaagttgt tgcccgtgtg aaagccattt
     8161 taagacgttc tcaatttgta aaagaagtag aagagattga cgatgatatt actatcggat
     8221 cgatacgtat tagacccgat tattttgaag tatataagca taatgagcta ttggagctaa
     8281 caccgaaaga atttgaactg ttgctttatt tgatagaaag acaaggcaga gtcattacga
     8341 gagagcatat gttaaatacg gtatggaatt atgaattcgc aggagactca agaattgttg
     8401 atgtacatat cagtcattta agagataaat tagaagataa tcctaagaag ccacaactta
     8461 ttaaaactgt gcgtggctta ggctataaat tggaaagacc taaagaacaa tgatgaagtt
     8521 tcaccaccgc ttaatgttat tgataagcag tatattaatt atcagttttg ttacattagg
     8581 cattattatt cacgcttcca tcacatatgt tttatcagaa aaacacgaac aagctctagc
     8641 aacagatgca agatcatata tttatttagt ccaagataat gagattgata aagttaaaga
     8701 aattgtaaaa cagcaaaata tagatttatt gataactaaa aatgatgaaa aggtgtttag
     8761 tagtggcaaa ctgaaagcta tcccaaatag tgatagcttt attcaaaaaa aggacgtttt
     8821 tatttttaat aagaaggtta acggttatca tgtatggatt aaaggttaca ataacgatat
     8881 tacagaaatg cattggacgt tgtggaaata tcttatattg acatgtttag ttgtacttat
     8941 ctgtctgtat tttgcaagcc gtagttttaa gcgtacacta atccgtccta ttcaagaagt
     9001 tacttatgca acacaattgt tggcaaatgg ttattatcac atacgtgtac ctgaaagtaa
     9061 tgttgtagaa actaaagcgc tttttgtgtc taccaatgat ttagcacgac gccttcaaaa
     9121 gcttaataat gagcaaaaaa ttcaatccaa ccgtttaaaa acaacgattg aaaatattcc
     9181 aagtgctatt ttaatgatag atcgaaatgg aaaaatcgtt gttgcaaata aagcgtatta
     9241 cgaacagttc aacatctcgc ataatattga acaagtaggc tatcacggct atgttaatac
     9301 tgaaattgag caattaattt tagaaagttt taaagttgaa aaacctatct atgaacaatt
     9361 ggaagttgca ataaaccaag tccatgctaa gtactttgac atttcatgcg ttccgatttt
     9421 aactaggtct caaaaaagtt tacaagggat tttagttgtg atgcatgaca ttacaaatct
     9481 gaaacaactt gaaaatttaa gacgtgaatt tgtagcaaat gtttcacatg aattaaaaac
     9541 acctattact tcaattaagg ggtttgctga aacgcttatt gatggtgcaa aaaatgatgc
     9601 ggaatcatta gacatgtttt taaacataat tttaaaagaa tcaaatcgaa tagaatcttt
     9661 agtaacagat ttgttagatt tatcgcatat tgagcaacat accgagttag atacagacta
     9721 tatgaattta tcagatttaa cgcggcgtat tattgataat atgatgactc aagccaatca
     9781 aaaaaatatt tccattcata ctgatattga aaaagatgtc attgttaaag cacaagaaag
     9841 taaaattgct caagttatca cgaatttgtt aacgaatgca attaactatt cttatgaaga
     9901 tggagatatt aacgttcgtg tgtatcgaga tgactttcgt gtcattttcg aagtacagga
     9961 ttttggtata ggtattaaat tagaagacca acaacgtatt tttgaaagat tttatagagt
    10021 agataaagca cgaagtagag attctggtgg gacaggtctt ggattatcaa ttacaaagca
    10081 tatagtagag gcccaccaag gcaatattga agtgaatagt caagttggca aaggctcaac
    10141 gttcaaagtt attctaaaag attataaaga ataaaaatta aagtggtaac agcgcgtgta
    10201 tttttcacga agttgttgct gcttttttat ttcgttcaaa tctctattgg tattaaatta
    10261 gagttaatga agtggaataa actcggttgt caattttgtc atttgcatat gtcgttttgt
    10321 aagttataag taatagattg ttgtgaatca agcaatttta taatatattt attataataa
    10381 agaaatttta gtaatgcgct taaaatagat acttaattaa aaagtaataa gttttatttg
    10441 aacggaaata aatcttaatt taaacacttg aatagacaag tgtaatcatt cgtcatagaa
    10501 aaagacgagg ggcgatttag ataaattgtt gaaaagtaat attaaatacc ttgtttatct
    10561 aatttgattg aggaaacatg cataggtgaa aattagaaat acattataaa tattgaatta
    10621 atttttcgac ctacaagttg aggaaaattt aactagattt gaggattagc aaagtgaaaa
    10681 caattcaatt acacaagaca actttgatat taattgcgat attattgttt tatactttta
    10741 tgggtatctt actaccactc atgcacgatg atttacaatg gtttagcaat tataatactg
    10801 atattttaaa agtaggattt gcatcactta atggtcgcta tatcggaaat atctttgaaa
    10861 ttatagctgt acatgtaagc tggttacgtt ggctttcata tggtctcatt agtatgggga
    10921 tcatttggat gattatgcac attacacgtt gtaaagcgtg gacaagctat tatttgttag
    10981 cattttcttt aatgttaatt ttaccaagtg ctatttatgc agatacattt ggctggtttg
    11041 caggatttta caattatgcg acatcaacac taatttcact ctttatcatt tattattgta
    11101 ttaatgcaat tatatataaa gaaaagcagc cggtaagtgt tacagtacta ttctatgttt
    11161 tatgcttctt cggtcaatta tttatggaga atgtgacatt attccactgt atcattttaa
    11221 ttttagcttt tctgtatgaa tttactgcaa atcgaacact taattataaa ttattgtttt
    11281 catttatgat tgctactata ggtacaatca ttatgttttc caatccaaat tatcgcaaaa
    11341 ttttatttga aggatcagaa tatcaacaag tttctaataa tcaaggtatc ttttcaaaat
    11401 ttgcagaaat gctatcaacg tcacttccat atggtgtcat tttcagtcag gttataattt
    11461 taagtatgat agcagcgctt attatttatc tactgcttag aagtgatcgg tatgtacatt
    11521 taacaatatt aaagcgtcgt attattatga taggatttat cactttaccg ctttattatt
    11581 tactatttta caatcagttt ttattaaata aaaatacaga tattggattg gttggttttg
    11641 cgaacgtaat tgtatgtggt tactttgcgt tttcgatatt tgtaggtatt tatttgtcta
    11701 ttaatgatcg taaaacacaa attactttat attccttact tattgcaata tgtgtttctg
    11761 cgtcgacact agtcattgtt actccaattg aaccaggtaa ttttttgatt gtttatacga
    11821 tacatgtaat cattttaatt attttagtaa aagaatttcg gaaatataag tcaatcaatg
    11881 ttaattttat taaagttgca tcactagttt tagctatgat atatcttagt gcatttattt
    11941 atgttcatta tgaacatgaa aaaagagttc agttattaaa acaacaaatt aaagatcatc
    12001 cacgacaaga ggtttataca gtggaatcat tgccgtttga acattatatg catcatccgt
    12061 caccaatcaa taaaaattac caagagtggt ttaataatta tgaagaatta ccgaaaatga
    12121 ccaaagttaa atatattcca tttggttctg acgattcata acttattctt acctataaaa
    12181 atttaaaata gcagcataaa aatattaaaa agtagacata atcatgaaat gataatgtgc
    12241 atctcaaaag ttggactttt aacctgactt tatggagtgc tcatcaagaa caatcatgat
    12301 tatgtctttt tattttttag cttagtctat atagtcactt gttttatttg aaattaggtc
    12361 gtgtataaat actctatgag atagtttctt taattacgac atcacagtcg aatatgctaa
    12421 aatatatata aattagttct ggaggttaaa attgtgaata aattagtatt aatcgatggt
    12481 aatagcttaa gttttagagc tttttatgca ttacctttgt tatcaaacaa agcaggcatt
    12541 cataccaatg cagtctatgg ttttgcaatg ttactagaga agattttaaa agaagaaaag
    12601 ccaaatcatt ttttagttgc gtttgatgca ggtaaaacga ctttcagaca tgaaaaatat
    12661 agtgaatata aaggtggacg ccagaaaacg ccgcctgaac taagtgagca atttccttat
    12721 atacgccaat tattagatgc ttatcatatt aaacgttatg aattagataa ctatgaggca
    12781 gatgatatta tcggaacttt aagtaaagaa gcagataaag cgggctttca gacaattatt
    12841 attacgggag accgagattt aacacaactt gcaacggata atgtaaccat ttattacact
    12901 aaaaaaggtg ttactgatgt tgatcattat acacctgact ttattgcgga aaaatacaac
    12961 gggctaacac ctaatcaaat tattgatatg aaaggattaa tgggagatac ttctgataat
    13021 ataccaggtg ttgcaggagt tggagagaaa acagcaataa aattgctgaa ccaatttgac
    13081 acagtagaag gtgtctatga acatttagat gaaatttcag gtaaaaagtt aaaagaaaaa
    13141 cttcaaaata gcaaagaaga tgcattaatg agtaaagaat tagcaacgat taatgtagat
    13201 agtccgattg aagtaaaact tgaagatacg ttaatgactc atcaagatga acaacaagaa
    13261 aaaatcgaat tgtttaagaa gttagaattc aaacaattgt tggcagacat tgatcaatca
    13321 gcaagcgttg aagatgcaat agaaaagaca tttgaaattg aaacgtcttt tgataatatt
    13381 gattttactt cattgaaaga agcggccatc catttcgaat tagacggtgg taactatttg
    13441 cgaaataata ttttaaaatt ctctttattt acaggtgaga aacatattgt aattaatgcg
    13501 gatgacataa ataattatgt cgaactagtt tcatggttag aaaatccgaa tacgaaaaaa
    13561 gtcgtatatg atgctaaaaa aacatatgta gcatcacata gattggaaat tgatattcaa
    13621 aatatttcat tcgatattat gttggccagt tatataattg atccatctcg tacgattagt
    13681 gatgttcaat cagttgtttc attgtacggt caaagttttg tgaaagacga tgtgagtata
    13741 tatggaaaag gtaagaaatt taaggtacct gaagatgatg ttttaaatcc atatgttgct
    13801 tctattactg atgcaattta ctttgcgaaa ccaaatatgg ataaacaatt agaagaatac
    13861 aatcaggtag aactcttagc tgatttagag ctaccgctag ctaagatttt aagtgaaatg
    13921 gaagaaatcg gtatatatac agatgttcat gatttagaag aaatggaaaa agaaattcaa
    13981 gaaaaattag atgtcttgat tcgaaatatc catgatgcag ctggtgaaga ttttaatata
    14041 aattctccta agcaattagg tgttgtattg tttgagacat tacaattacc tgttattaaa
    14101 aagacgaaaa caggatattc tactgctgta gatgtcttag agcaattgca aggtgaacat
    14161 cctatcattg attatatttt agaatatcgt caattatcta agttacagtc tacttatgtt
    14221 gaaggattgc aaaaggtaat tagtgatgat caacgtatcc atacacgttt taaccaaact
    14281 ttagcgcaaa ctggacgttt atcaagtgta gatcctaatt tacaaaatat tccggttaga
    14341 cttgaagaag ggcgtaaaat aagaaaagcc tttaaaccaa cttcaaaaga tagcgttata
    14401 ttatcagcag attattctca aattgaattg cgtgtattag cacacattac acaagatgag
    14461 agtatgaaag aagcatttat caacggcgat gatattcata cagcaactgc tatgaaagta
    14521 tttggtgtag aagctgatca agtcgatagt ttaatgcgtc gtcaagcaaa agcggttaac
    14581 tttggaattg tttatgggat aagtgattat ggtttaagtc aaagcttagg tattactcgt
    14641 aaaaaagcaa aagcattcat tgatgattat ttagctagtt tcccaggtgt aaaacaatat
    14701 atgtctgata ttgtaaaaga tgccaaagct ttaggttacg tggaaacatt gctacatcgt
    14761 cgacgttata ttcctgatat tacgagtcgt aactttaatt tacgcggctt tgctgaacgt
    14821 actgctatga atacgccaat acaaggcagt gctgcagata tcattaaact ggcaatggtt
    14881 aaatttgcgc aaaaaatgaa agagacaaca tatcaagcta aactattatt acaagtgcac
    14941 gatgaattaa tttttgaagt acctaagtca gaagtagatt catttagtga atttgtagaa
    15001 gagataatgg aaaatgcatt gcaattagat gttccattaa aagtagactc aagttatggt
    15061 gcaacttggt atgatgcaaa ataaagtgag gttaatggta tgcccgaatt accagaagta
    15121 gaacatgtaa aaagaggaat tgaaccctac gttataaatc aaaaaattga acacgttata
    15181 ttttcggata aagtaatcga aggtaaagat caaggcaagg aaacaattat aaaaggaatt
    15241 gaattagata cttttaaaac tttatcggaa ggctatacca ttactaatgt aaaacgaaga
    15301 agtaaatata tcgtttttca actagataat aaacgagaaa aacgtatatt aattagccat
    15361 ttaggtatgg cgggtggttt tttcattgta gatgaacttg aagatattat gatacctaat
    15421 tatcgtaagc attggcatgt tatttttgaa ctgtcaaatg acaaaaagtt aatttattca
    15481 gatatacgtc gttttggaga aattagaaat gttgcgtctg tagcatctta tccttcattt
    15541 ttagaaatag cgcctgaacc tttttcaaat gaagcattga cgtattattt aaatcgaatt
    15601 catcaacagt ctaataagaa taaaccaatc aaacaagtga ttttggatca taaagtaatc
    15661 gcaggttgcg gaaatattta cgcatgtgaa gcgttgtttc gtgcaggtgt tttaccagat
    15721 aaaaaaataa aagacttaac ataccaacaa caagaaatgg ttttttatta tgttagagaa
    15781 gtactagaag aaggtattaa acatggcggt acaagtattt cagattatcg tcatgctgat
    15841 ggaaaaactg gtgagatgca attgcatcta aatgtatata agcaaccggt ttgcaaagtg
    15901 tgtggaagtc aaattgaaac aaaaatcatc gcgacaagaa atagtcatta ttgtcctgta
    15961 tgtcaaaaat aaagaaagag tgaaattatg ccgaaagtta ttggtctaac aggtggaatc
    16021 gcctcaggaa aatcaacagt atcagaactc ttatccgtat tcggttttaa agtagtagat
    16081 gctgataaag cagccaggga agctgttaaa aaggggagta aaggtttagc tcaagtacga
    16141 gaagtctttg gtgatgaagc aattgatgaa aatggtgaga tgaatcgtcg ttatatgggg
    16201 gatttagtgt ttaaccatcc agaaaaacgc ttagaattaa atgctatcat acatcctatc
    16261 gtgcgagata ttatggaaga agaaaagcaa gaatatttaa aacaaggata taatgtaatc
    16321 atggatattc cattattatt tgaaaatgaa ttggaaaata cagtagacga agtgtgggtt
    16381 gtatacactt ctgaaagtat acaaatggat cgtttaatgc aacgtaataa tttgtcatta
    16441 gaagatgcga aagctcgtgt ctatagccaa atttctattg ataaaaaaag ccgaatggcc
    16501 gatcatgtta tcgataattt aggggataaa cttgaattaa aacaaaacct tgagagattg
    16561 ttaaaagaag aaggttatat tgaaaagccg aattacggag aagaagatta atattacact
    16621 ataaataagt cattacttta cgtacgcttt gatgtatgta agtaatgact attttttata
    16681 aaaaagataa ataaatcaac ggaaaacgct ttcaaatttc atataatatg ctatactaat
    16741 tccataaagt ataacacata aaaatcaagg ggtgctttta atgtcaacga atattgcaat
    16801 taatggtatg ggtagaattg gaagaatggt attacgtatt gcattacaaa ataaaaattt
    16861 aaatgtagta gcgataaatg ctagttatcc acccgaaaca attgcacatt taatcaatta
    16921 cgatacaaca catggaaaat ataatctaaa agttgaaccg attgaaaatg gattgcaagt
    16981 tggtgatcat aaaattaaat tggttgctga tcgcaatcct gaaaacttgc catggaaaga
    17041 attagatatc gatattgcta tagatgcaac tggtaaattt aatcatggtg ataaagccat
    17101 cgcacatatt aaagcaggtg ccaaaaaagt tctgttaact ggtccttcaa aaggtggaca
    17161 tgttcaaatg gtagttaaag gcgtaaatga tgaccaatta gatatagaag catttgacat
    17221 ttttagtaat gcttcatgta ctactaattg cattggtcca gttgcaaaag ttttaaataa
    17281 tcagtttgga attgttaatg gtttaatgac tactgttcac gctattacaa atgaccaaaa
    17341 aaatattgat aatccacata aagatttaag acgtgcacgc tcatgtaatg aaagcattat
    17401 tcctacttct actggtgcgg cgaaagcttt aaaagaagta ttaccagaat tagaaggtaa
    17461 attacacggc atggcattac gtgtaccaac aaagaatgta tcgctcgttg atttagttgt
    17521 tgatttagaa aaagaagtaa ctgcagaaga agtaaaccaa gcttttgaaa atgcaggttt
    17581 agaaggtatc attgaagtcg aacatcaacc actagtgtct gttgatttca atactaatcc
    17641 acattcagct attattgatg caaaatcaac aatggtcatg tcagggaata aagtaaaagt
    17701 tatcgcttgg tatgataatg aatggggtta ttcaaataga gttgtagatg ttgctgagca
    17761 aattggagca cttttgacat caaaagaaac tgtaagtgca agttaataca attaatttat
    17821 agcctaactc tgacttcgga gttaggtttt tatttttaat acaataatat tgtcatgcat
    17881 attactaaat aagtaacgat ataatgtttt ttattgtttc attatttggt taaatgaatt
    17941 aaattg
//
LOCUS       DAHHRR010000044        17360 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.044, whole genome shotgun sequence.
ACCESSION   DAHHRR010000044 DAHHRR010000000
VERSION     DAHHRR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17360
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.044"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            338..1489
                     /locus_tag="NBI42_001331"
     CDS             338..1489
                     /locus_tag="NBI42_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HCD1777754.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLSNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETVLIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNLEFKTEKFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
     gene            1654..2697
                     /gene="recA"
                     /locus_tag="NBI42_001332"
     CDS             1654..2697
                     /gene="recA"
                     /locus_tag="NBI42_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCD1777755.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQFKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            3051..4610
                     /gene="rny"
                     /locus_tag="NBI42_001333"
     CDS             3051..4610
                     /gene="rny"
                     /locus_tag="NBI42_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCD1777756.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(4623..4685)
                     /locus_tag="NBI42_001334"
     CDS             complement(4623..4685)
                     /locus_tag="NBI42_001334"
                     /inference="COORDINATES: protein motif:HMM:NF012587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HCD1777757.1"
                     /translation="MKCCLTRLRLQLKKSSLTNL"
     gene            complement(4908..5123)
                     /locus_tag="NBI42_001335"
     CDS             complement(4908..5123)
                     /locus_tag="NBI42_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777758.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            5297..6094
                     /locus_tag="NBI42_001336"
     CDS             5297..6094
                     /locus_tag="NBI42_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCD1777759.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            6234..7994
                     /locus_tag="NBI42_001337"
     CDS             6234..7994
                     /locus_tag="NBI42_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HCD1777760.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIDGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYTLTSNGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            7995..8861
                     /locus_tag="NBI42_001338"
     CDS             7995..8861
                     /locus_tag="NBI42_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HCD1777761.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            8956..9249
                     /locus_tag="NBI42_001339"
     CDS             8956..9249
                     /locus_tag="NBI42_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCD1777762.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            9383..10927
                     /gene="miaB"
                     /locus_tag="NBI42_001340"
     CDS             9383..10927
                     /gene="miaB"
                     /locus_tag="NBI42_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCD1777763.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLDVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFVKEVE
                     PEMVIQ"
     gene            10928..11293
                     /locus_tag="NBI42_001341"
     CDS             10928..11293
                     /locus_tag="NBI42_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HCD1777764.1"
                     /translation="MYNKDDVLKQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            11320..11811
                     /gene="thiW"
                     /locus_tag="NBI42_001342"
     CDS             11320..11811
                     /gene="thiW"
                     /locus_tag="NBI42_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCD1777765.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIMSYFLLITLKKRGILQR
                     FIK"
     gene            12114..14732
                     /gene="mutS"
                     /locus_tag="NBI42_001343"
     CDS             12114..14732
                     /gene="mutS"
                     /locus_tag="NBI42_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCD1777766.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDHLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQDTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNMQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALTSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENSNLSVKETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            14745..16754
                     /gene="mutL"
                     /locus_tag="NBI42_001344"
     CDS             14745..16754
                     /gene="mutL"
                     /locus_tag="NBI42_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCD1777767.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMAENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDKDYFKKQQEILQEMDQTFDSNDDTSVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            16760..17302
                     /locus_tag="NBI42_001345"
     CDS             16760..17302
                     /locus_tag="NBI42_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCD1777768.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
BASE COUNT         6547 a         2345 c         3263 g         5205 t
ORIGIN      
        1 atctagtttt ttttaatccc tattttagaa attagccacg caattgttta taatgatggg
       61 agtgggacag aaatgatatt ttcgaaaaaa ttatttcgtc gtcccacccc ggcaaggttg
      121 actagaattg aaaaaagctt gttacaagcg tattttcgtt cagtcaacta ctgccaatat
      181 aattttgtag agcattgaac attgatttat gtctcaggct cttgttcatt tttttaggaa
      241 aatgtgttgt agcatctgat acattgaatt taaaattgat gtgaattttt aaatggaata
      301 catgaaaaaa gaattaaacg atacaagggg gatataaatg tcaattgcca ttattgctgt
      361 aggctcagaa ctattgctag gtcaaatcgc taataccaac ggacaatttc tatctaaagt
      421 atttaatgaa attggacaaa atgtattaga acataaagtt attggagata ataaaaaacg
      481 tttagaatca agtgtacgtc atgcgttaga aaaatatgat actgttattt taacaggtgg
      541 cttaggtcct acgaaagatg acttaacgaa gcatacagtg gcccagattg ttggtaaaga
      601 tttagttatt gatgagcctt ctttaaaata tattgaaagc tattttgagg aacaaggaca
      661 agaaatgaca cctaataata aacaacaggc tttagtaatt gaaggttcaa ctgtattatc
      721 aaatcatcat ggcatggctc caggaatgat ggtgaatttt gaaaacaaac aaattatttt
      781 attaccaggt ccaccgaaag aaatgcaacc aatggtaaaa aatgaattgt tgtcacattt
      841 tataaaccat aatcgaatta tacactctga actattaaga tttgcgggaa taggtgaatc
      901 taaagtagaa acagtattaa tagatcttat cgataaacag actaatccta cgattgcgcc
      961 tttggcagga agtcatgaag tatatattag attgactgca aatgccgact caaaagaaca
     1021 agcacaatca ttgattcaac ccgttaagca agaaattctt gatcgtattg gagaatatta
     1081 ttatggttca gatgacacat taattgagca agctgtaata aagaaaattc atgaaccttt
     1141 tgtaatatat gatggtatta ctaatggtgc tttatatcat cgattgaaag aagtggattt
     1201 aaacgatgtt ctaaagggta tgattaatca caatgaaaac tttgttgata ttaataaacc
     1261 tattgagcag caattaaaag atgcagtgca atttgttaat aaattattta atgtgtcatc
     1321 agcaattatt ctattagagt atgatggtgt agtccatata ggctatgata ataacttaga
     1381 atttaaaact gagaaattta aaatgtctaa atctagaaat ttattaaaga acagaagtca
     1441 aaattatgcg cttataagat tattaaattg gcttagaaca acaaattaat tgtattatcg
     1501 ataaaaatat aagcacgttt gttcgttttt gtgttttgat ttcaagattt tatacgaaca
     1561 aatattcgca aaacacttgt attttatttt gaatccttgt atagtattgg taagataatt
     1621 aaaagatagc aatttcaatt aggaggtctc gctttggata acgatcgtca aaaagcttta
     1681 gatacagtaa ttaaaaatat ggagaaatct ttcggtaaag gtgccgtaat gaagttaggt
     1741 gacaatatag gtcgccgagt ttcaactaca tcaactggtt cagttacatt agataatgcg
     1801 ctaggtgtag gtggctatcc taaaggacga attattgaaa tttatggtcc tgaaagttct
     1861 ggtaagacaa cagtagcgct tcacgctatt gctgaagtac aaagtaatgg cggggtggca
     1921 gcatttatcg atgctgaaca tgctttagat ccagaatatg ctcaagcatt aggcgtagat
     1981 atcgataatt tatatttatc gcaaccggat catggtgaac aaggtcttga aatcgccgaa
     2041 gcatttgtta gaagtggtgc agttgatatt gtagttgtag actcagttgc tgctttaaca
     2101 cctaaagctg aaattgaagg agaaatggga gacactcacg ttggtttaca agctcgttta
     2161 atgtcacaag cgttacgtaa actttcaggt gctatttcta aatcaaatac aactgctatt
     2221 ttcatcaacc aaattcgtga aaaagttggt gttatgttcg gtaatccaga gactacacca
     2281 ggtggacgtg ctttaaaatt ctatagttca gtaagactag aagtacgtcg tgcagaacag
     2341 cttaaacaag gacaagaaat tgtaggtaat agaactaaaa ttaaagtcgt taaaaataaa
     2401 gtggcaccac catttagagt agctgaagtt gatattatgt atggacaagg tatttctaaa
     2461 gagggtgaac ttattgattt aggtgttgaa aacgacatcg ttgataaatc aggagcatgg
     2521 tattcttaca atggcgaacg aatgggtcaa ggtaaggaaa atgttaaaat gtacttgaaa
     2581 gaaaacccac aatttaaaga agaaattgat cgtaaattga gagaaaaatt aggtatatct
     2641 gatggtgatg ttgaagaaac agaagatgca ccaaagtcat tatttgacga agaatagtac
     2701 acaaatttat atctatagtt aaacttagca aatatcctta taggattgat tgaaagtgat
     2761 attcatctca taaagctgga ataatatcta actttatggg atgcacttca aatcgagact
     2821 ataaggtttt ttattttatt tattattaca ttatcaatag ttttataatc gagcttcaaa
     2881 actttagaaa atagtagaaa tagcattcaa tatagtgcaa aagtgcaaat tgataacttg
     2941 acacttatct cctataaacc gtacaattaa tttgtatgat ttatatataa tttcataaag
     3001 tcatattgaa tttcatataa agagcaaacc ctagaaaagg aggtgtttgt gtgaatttat
     3061 taagcctcct actcattttg ctggggatca ttctaggagt tgttggaggg tatgttgttg
     3121 cccgaaattt gttgcttcaa aagcaatcac aagctagaca aactgccgaa gatattgtaa
     3181 accaagcaca taaagaagct gacaatatca aaaaagagaa attacttgag gcaaaagaag
     3241 aaaaccaaat cctaagagaa caaactgaag cagaactacg agaaagacgt agcgaacttc
     3301 aaagacaaga aacccgactt cttcaaaaag aagaaaactt agagcgtaaa tctgatctat
     3361 tagataaaaa agatgagatt ttagagcaaa aagaatcaaa aattgaagaa aaacaacaac
     3421 aagtagatgc aaaagagagt agtgttcaaa cgttaataat gaagcatgaa caagaattag
     3481 aacgcatctc cggtctcact caagaagaag ctattaatga gcaacttcaa agagtagagg
     3541 aagaactgtc acaagatatt gcagtacttg ttaaagaaaa agaaaaagaa gctaaagaaa
     3601 aagttgataa aacagcaaaa gaattattag ctacagcagt acaaagatta gcagcagatc
     3661 acacaagtga atcaacggta tcagtagtta acttacctaa tgatgagatg aaaggtcgaa
     3721 tcattggacg agaaggacga aacatccgca cacttgaaac tttaactggc attgatttaa
     3781 ttattgatga cacaccagaa gcagttatat tatctggttt tgatccaata agaagagaaa
     3841 ttgctagaac agcacttgtt aacttagtat ctgatggacg tattcatcca ggtagaattg
     3901 aagatatggt cgaaaaagct agaaaagaag tagacgatat tattagagaa gcaggtgaac
     3961 aagctacatt tgaagtgaac gcacataata tgcatcctga cttagtaaaa attgtagggc
     4021 gtttaaacta tcgtacaagt tacggtcaaa atgtacttaa acattcaatt gaagttgcgc
     4081 atcttgctag tatgttagct gctgagctag gcgaagatga gacattagcg aaacgagctg
     4141 gacttttaca tgatgttggt aaagcaattg atcatgaagt agaaggtagt catgttgaaa
     4201 tcggtgtaga attagcgaaa aaatatggtg aaaatgaaac agttattaat gcaatccatt
     4261 ctcaccatgg tgatgttgaa cctacatcta ttatatctat ccttgttgct gctgcagatg
     4321 cattgtctgc ggctcgtcca ggtgcaagaa aagaaacatt agagaattat attcgtcgat
     4381 tagaacgttt agaaacgtta tcagaaagtt atgatggtgt agaaaaagca tttgcgattc
     4441 aggcaggtag agaaatccga gtgattgtat ctcctgaaga aattgatgat ttaaaatctt
     4501 atcgattggc tagagatatt aaaaatcaga ttgaagatga attacaatat cctggtcata
     4561 tcaaggtgac agttgttcga gagactagag cagtagaata tgcgaaataa tttttgtctc
     4621 cctcacaaat tagtgaggga gcttttttta agttgtagtc tcaatctagt tagacagcac
     4681 tttattggta ataactatat taaacagtag ttatttgaaa gtaagacgga ccttatatta
     4741 aataagaagg tattgctttt aataaaaatg ttttaggctt cataattact atatttatat
     4801 tatgtaaacc tataaaggtg attgcttttc tatccaataa aaaagaagag aagatgtaac
     4861 acatcttctc ttctgtaata ttaattagga tttatttcta agttgagtta ttttaattgt
     4921 aaatctgttt tctttaattc ttttataact tctgcagtat cataacaatt tgttgcaatt
     4981 gttgaatatc tctctgctaa acgatatgca ttaatgtaaa gctttaaact ttctttagct
     5041 atatcctctg catcttcgaa ttttgatggg ttagacataa ccactaattc tgcaaatttt
     5101 tctggatcaa tattaataga catgtattta tttacaactc ctatttattt tgatgtctta
     5161 atactaacat attgaagttt tcagacaaag taatgtctct ctataattga agaaaaataa
     5221 ttcaaagaat ttaatattta ctttaaatat tagaatgtat gaattaaact agtatttatg
     5281 agaggatgaa caaaacatga gaataatgtt tataggggat atcgtaggta aaattggacg
     5341 agacgcaatt gaaacgtaca tacctcaact gaagcaaaag tataaaccaa cagttacaat
     5401 tgtaaatgct gaaaatgcag cacatggtaa aggtttgact gaaaaaatat ataaacaatt
     5461 actaagaaat ggtgtagatt tcatgactat gggtaatcac acatatggtc aacgtgaaat
     5521 ttatgatttt atagatgaag caaaacgact agtaagacca gcgaattttc cggatgaagc
     5581 gccgggaatt ggtatgagat ttatacaaat taatgatatt aaacttgcag ttattaatct
     5641 gcaaggaaga gcgtttatgc cagatattga tgatcctttt aaaaaggcag atcaattagt
     5701 caaggaagca caagaacaaa ctccgtttat atttgttgat tttcatgcag aaacaacttc
     5761 tgaaaagtat gcaatgggat ggcatctaga tggtagagct agcgctgttg ttggaacgca
     5821 tacacacatt caaacagcag atgaacgtat tttaccaaag ggtacagggt atataacgga
     5881 tgttggtatg acaggctttt atgacggcat tttaggaata aataaaacag aggtaattga
     5941 gcgttttatc actagtttgc cacaaagaca tgttgttcca aatgaaggca gaagtgtatt
     6001 atctggtgtt gttattgatt tggacaaaga aggtaaaaca aagcacatcg aacgtatatt
     6061 gataaatgat gaccatccat tttcaacatt ttaaaattac gtaagtaaac attcgaattg
     6121 gaccctatcg tccattagta tgaatttaat atagtaccac tgtttacata gtaaatcggt
     6181 ggttcttttt gttatcattt aatatgaaat atatccatag gaggcatata actatgaaac
     6241 cacaattatc gtggaaagtt ggcggtcaac aaggcgaagg cattgaatca actggggaaa
     6301 tcttcgctac ggctatgaat agaaaaggat attatttata tggatataga catttttcaa
     6361 gtcgtatcaa aggtggacat acgaataata aaattagagt ttctacgacg cctgttcatg
     6421 caattagtga tgatttagat attttgattg catttgacca agaaacaatt gatgttaacc
     6481 atcatgaaat gagagaagac agtatcattt tagctgatgc caaggctaaa cctgtgaaac
     6541 cagaaggatg tcatgcacag ctaattgaat taccttttac agcaaccgct aaagaattag
     6601 gtacagcatt aatgaaaaac atggttgcaa taggtgctac tagcgcattg atgaatttga
     6661 atacaaatac atttgaagaa cttattacta atatgttttc taaaaaaggt gacaaggtag
     6721 ttgaagtcaa tatccaagca ttaaacgaag gttatcaatt aatgcaatcc cgcttacctg
     6781 aaatcgacgg ggactttgaa ttagagtcaa cagatgcact accacatcta tatatgattg
     6841 gtaacgatgc cattggatta ggtgcaattg ctgcaggttc acaatttatg gcggcatatc
     6901 ctattacacc tgcgtctgaa gttatggaat atatgattgc caatatatct aaagtaaacg
     6961 gagcggttat tcaaacagaa gatgaaattg ctgctgtaac tatggctatt ggtgcaaatt
     7021 atggtggagt cagagcgttt acggctagtg ctggtccagg tttatcttta atgatggaag
     7081 caattggatt atctggtatg actgaaacgc cattagtcat tattaatacc caacgaggcg
     7141 gaccttctac tggattacct acgaaacaag aacagtcaga tttaatgcaa atgatttatg
     7201 gtacacatgg tgatattcca aaaatagttg tagcaccaac tgatgcagaa gatgcatttt
     7261 atttaactat ggaagccttt aatttagcag aacaatatca atgccctgtt atagttctaa
     7321 gtgatttgca attatcttta ggtaaacaaa ctgttgaaaa attagattat aatcgtattg
     7381 aaattaaacg tggtgaaatc attcaatctg atattgaacg tgaagaagat gataaaggtt
     7441 atttcaagcg ttatacgtta acatccaatg gtgtttctcc tagacctatc cctggtgtta
     7501 aaggaggtat tcatcatata actggtgtgg aacacaatga agaaggtaaa cctagtgaat
     7561 ctgcgtcaaa tagacaacaa caaatggaaa aacgaatgcg taaaattgag cagttactaa
     7621 ttgaatcgcc agtagaagct aacttacaac atgaggatgc agatattctt tatatcggtt
     7681 ttatttctac aaaaggtgca attcaagaag gtagtaaccg tttgaatcaa caaggcataa
     7741 aagttaacac tatacaaatt agacaattgc atccattccc aacaagcgtt attcaagatg
     7801 cagttaataa agcgaagaaa gtcgttgtag tggagcacaa ttatcaagga caattggcta
     7861 gtattataaa aatgaatgtc aatattcatg ataagattga aaattataca aagtatgatg
     7921 ggacaccttt cctaccacat gaaatcgaag aaaaaggtaa aataattgct actgaaataa
     7981 aggagatggt atagatggcg acatttaaag attttagaaa taatgttaag cctaactggt
     8041 gccctggatg tggcgatttc tcagtacaag ctgcaattca aaaagcagcc gcaaatatag
     8101 ggttagaacc tgaagaagta gctatcatca ccggtatagg atgttctggc cgtctttcag
     8161 gatatattaa ttcttatggc gttcattcta ttcacggacg tgcattacct ttagctcaag
     8221 gtgtaaaaat ggcgaataaa gatttaactg ttattgcatc tggaggagat ggtgatggtt
     8281 atgctatagg tatgggacat acaatccatg ctttaagaag aaatatgaac atgacgtata
     8341 tagtcatgga taatcaaatt tatggtttga caaagggaca aacatcgccg tcatcagcag
     8401 taggatttgt tactaaaaca acgccaaaag gtaatataga aaaaaatgtt gcgcctttag
     8461 aattagcatt atcatctggt gccacatttg tagcccaagg tttttcaagt gatattaaag
     8521 gattaacaaa actaattgaa gatgcaatta atcatgatgg attttcattc gtaaatgtct
     8581 tttcaccatg tgtgacttat aataaaatta acacatacga ttggtttaaa gaacatttaa
     8641 caagtgttga tgacattgaa aactatgatt ctacagataa acaattagcg actaaaactg
     8701 ttattgaaca tgaatcttta gtaactggta ttgtttatca agataaagaa acaccatcat
     8761 atgaatctca aattaaagag ttagatgata caccacttgc taaaagagat atcaaaatta
     8821 ctgaagacac gttcaatgca ttaactgaac aatttattta ataattaaaa ctgagtcgta
     8881 taaatgtatt tataacagat ccatttatgc tactcagttt tttactatta caaaaaaata
     8941 aaggagtttt taaaaatgaa agacacacta atgagtatac aaataattcc taaaacacca
     9001 aacaatgaca atgttatacc ttacgtagac gaggcgatta aaataattga cgaatctggt
     9061 ttgcatttta gagtaggtcc gttagagacg acagtacaag gaaatatgaa tgaatgttta
     9121 attttaatac aatcattaaa tgaacgaatg gtggaacttg aatgtccaag tattattagc
     9181 caagttaagt tttatcatgt gccagacggc atcactattg aaactttaac tgaaaaatat
     9241 gatgaataac attaaaagtg aagtaaactg gatttgaatt ggcttgttag agatgacgta
     9301 taactttaac tgtttttgca ctttatagtt aaatttaata taattattaa atgatacggg
     9361 caaatagaaa ggattttgta aagtgaacga agaacaaaga aaagcaagtt ctgtagatgt
     9421 tttagctgag agagataaga aagcagaaaa agattatagt aaatattttg aacatgttta
     9481 tcaaccgcct aatttaaaag aagcgaaaaa aagaggtaaa caagaagttc gttataatag
     9541 agatttccaa attgatgaaa aatatcgcgg tatggggaac gagcgtacat ttttaattaa
     9601 aacatatgga tgtcaaatga atgcacatga cactgaggtc attgctggta tacttgaagc
     9661 attaggctat caagcaacga ctgatattaa cactgcagat gttattttaa ttaatacatg
     9721 tgcgattaga gaaaatgccg agaacaaagt gtttagtgaa ataggtaatt tgaagcattt
     9781 gaagaaagaa cgacctgata ttttaatcgg agtttgtggt tgtatgtcac aagaagagtc
     9841 agtagtgaat aaaattttaa aatcgtatca aaatgtagat atgatttttg gtacacataa
     9901 tattcatcat ttaccagaaa ttttagaaga agcatactta tctaaagcaa tggttgttga
     9961 agtatggtct aaagaaggag acgttattga aaatcttcca aaagtccgtg aaggcaacat
    10021 taaagcatgg gtcaatatta tgtatggttg tgataagttt tgtacatatt gcattgttcc
    10081 atttacaaga ggtaaagaac gaagccgtag acctgaagat attatagatg aagtacgtga
    10141 gcttgctcgt gaaggttaca aagaaataac gcttttaggt caaaatgtaa attcttacgg
    10201 taaagattta caggatatag aatatgactt aggagatctt ttacaagcaa tttctaaaat
    10261 agcgattcca agagttcgtt tcacaacaag tcatccttgg gacttcacag atcacatgat
    10321 tgatgttatt tcagagggcg gtaatatcgt tcctcatatc cacttgccag ttcaatctgg
    10381 aaataatgca gtattaaaaa taatgggtag aaaatataca cgagaaagtt atttggattt
    10441 agtaaaacga atcaaagata gaattccaaa tgtagcatta actacagata ttattgtagg
    10501 gtatccaaat gaatcagagg aacaatttga agaaacttta actttatatg atgaagttgg
    10561 ttttgaacat gcatatacgt atttgtattc acaacgtgat ggtacgcctg ctgctaaaat
    10621 gaaagataat gtacctttag atgtcaaaaa ggaacgattg caacgtttga ataaaaaagt
    10681 tggtcattat tcacaaatag ctatgagtaa gtacgaagga caaactgtaa cagtactttg
    10741 tgaaggtagt agtaaaaaag atgatcaggt tcttgctggc tacaccgata aaaataagtt
    10801 agttaatttc aaagcgccta aagaaatgat tggtaaacta gtggaagtac gaatagatga
    10861 agctaaacag tattcattaa atggcagttt tgtaaaggaa gtagagccgg aaatggtgat
    10921 tcaataaatg tataataaag atgacgtgtt gaaacaagcg gataatattg caaataaaat
    10981 taaaaatttg gatactatca aaacatatca acaaattgaa gcacagattc atcagaacca
    11041 aacgataaag actaaaatgg atatgttaaa aaagcatcaa aaacaagcag taaactttca
    11101 aaattacggg aaacaaaatg cgctagaaca gtcggaacat accattcaaa gtatagaagc
    11161 agaaataaat acattgccca tagttgaaca gtttcaaact tcacaatatg aagcgaatca
    11221 attattgaaa atgtttgtat caacaatgga aacacgttta aatgaccata ataaagccaa
    11281 acatagtgat taattacaaa caaaaaggag aacaatcata tgaaatcaag aaaactggct
    11341 ataactgcac ttttaattgc aataaatgtt gtattaagca gtattatcat cattcctcta
    11401 ggaccagtta aggcagcacc agtacagcat tttgtaaatg tattaagtgc ggtcatagta
    11461 ggtccttggt atggattagc tcaagcgctt atatcatcaa ttttaagagt tctttttgga
    11521 actggtacag cttttgcatt tccgggtagt atgattggag ttttattggc tagtatgttt
    11581 tacatatatc gtaaacatat attcatggcc gcggtcggtg aagtacttgg aactggtgtc
    11641 atcggaagtt taatttgtat accattagca tatttccttg ggcttcaaga cttcttcatt
    11701 aaaccgttaa tgattacgtt catagtctca agtgctatcg gatctattat gagttatttc
    11761 ttattaatta ctctaaaaaa acgtggtatt cttcaaagat ttataaaata attttatgat
    11821 atatgatttt aaacagagtc attgttcatt ttaatattga acgcccttac ttaattaaat
    11881 gtcttgaatg ttagaatttt aatataattc taaacatgct attaacacat tttattaggt
    11941 ttgaggcgtt ttcttataca tttaagatgc ttcgttatta tatggtatga ataggtttgt
    12001 aaaagtaagt atgttaaaat gtataagtta aataattaat aattagaaag cttacaaacg
    12061 aatatattgt aaagacaaat tttaaaaaga gacaagggtt gtgaagaaat aatatgtcta
    12121 atgttacacc aatgatgcag caatatttaa aaataaaatc agaataccaa gattgcttat
    12181 tattttttag actaggtgat ttctatgaaa tgttttatga agatgccaag gaggcatcac
    12241 gtgtacttga aattacttta actaaaagag atgctaaaaa agaaaatcca attccgatgt
    12301 gtggtgttcc gtatcattct gcagatagtt atatagatac actagttaat aatggatata
    12361 aagtagctat ttgtgaacag atggaagatc cgaaacaaac gaaaggtatg gttagacgtg
    12421 aggtagtaag aattgtgact ccaggaactg tgatggagca aggtggtgta gatgataaac
    12481 aaaataacta tattttaagt tttgttatga atcaacctga aattgcgctt agttactgtg
    12541 atgtttctac tggcgaatta aaggttacac attttaatga tgaagcgact ttattaaatg
    12601 aaattacgac gataaaccct aacgaagttg ttatcaatga caatatttcc gatcatttaa
    12661 aaagacaaat taatatggtg acagaaacaa taacagtcag ggaaacgtta tcatcagaaa
    12721 tctatagtgt gaatcaaact gaacataaat taatgtatca agcgacacaa ttattgctag
    12781 attatattca tcatacacaa aaacgtgatt tatcgcatat cgaggatgtt gttcaatatg
    12841 cagctataga ttatatgaaa atggattttt atgctaagag aaaccttgag ttaacggaaa
    12901 gcattcgatt aaaatccaaa aaaggaacgc tactttggct gatggacgaa acgaaaacgc
    12961 caatgggagc acgtcgatta aaacaatgga tagatagacc actaataagt aaagaacaaa
    13021 ttgaagcacg attagatatc gttgatgaat ttagtgctca tttcatagaa agagacacct
    13081 taagaacata tcttaatcaa gtgtatgata ttgaacgtct tgttgggcgt gttagttacg
    13141 gaaatgttaa tgcgagagat ttaattcaac ttaaacattc catttctgaa ataccgaata
    13201 ttaaagcatt actaaattct atgaatcagg atactcttgt acaagttaat caactagagc
    13261 cacttgatga tttacttgat atattagaac agagtttagt agaagaacca ccaatttcag
    13321 ttaaagatgg tggactattc aaagttggtt ttaatatgca attagatgaa tatcttgaag
    13381 cttcaaaaaa cggaaaaaca tggttagcag aactacaagc caaagaaaga caacgtacag
    13441 gaataaaatc attgaaaata agctttaata aagtgtttgg ttattttata gaaataacac
    13501 gtgccaactt gcaaaatttt gaaccaagtg aatttggtta tatgaggaag caaacgttat
    13561 cgaatgcgga acgatttata actgatgaac ttaaagaaaa agaagatatc attttaggtg
    13621 cggaagacaa agccattgaa ttagaatatc aattatttgt tcagctacgt gaagaagtta
    13681 aaaaatatac tgaacgttta caacaacaag ctaaaattat ttcagagtta gattgtttac
    13741 agagctttgc agaaattgct caaaaatata attatactag gccttcattt agtgaaaata
    13801 aaacattaga attagtggaa tctaggcacc cagtagtgga aagagtaatg gattataatg
    13861 actatgtgcc taataattgt cgattagata atgaaacatt tatatattta attacaggtc
    13921 cgaatatgtc tggtaaatcg acgtatatga gacaagttgc cataattagt ataatggccc
    13981 aaatgggagc ttatgtccct tgtaaagagg ctgtgttacc tatatttgat caaatattca
    14041 ctagaatagg tgcggcagat gatttggttt caggtaagag tacgtttatg gtagaaatgc
    14101 tagaagcaca aaaggcatta acttatgcaa cagaggatag tttgattatt ttcgatgaaa
    14161 ttggacgtgg tacttcaacg tatgacggtt tagctttagc gcaggcaatg atagagtatg
    14221 tagctgaaac atcacatgct aaaacgttat tttcaacaca ttatcatgaa ttgacaacat
    14281 tagatcaagc attaacaagt ctaaaaaatg ttcacgtcgc tgctaatgaa tataaaggtg
    14341 aacttatatt cttgcataaa gtcaaagatg gtgcagttga cgatagttat ggtattcaag
    14401 ttgcgaaatt agctgattta cctgaaaaag ttattagcag agcacaagtg atcctaagcg
    14461 agtttgaagc gtctgctggt aaaaaatcat cgatatcaaa tttaaaaatg gtcgaaaatg
    14521 aacctgaaat taatcaagaa aattcaaact taagtgttaa agaaacaact gatacgttat
    14581 ctcaaaaaga ctttgaacaa gcatcatttg atttgtttga aaatgatcaa gaaagcgaga
    14641 ttgaactaca aattaaaaat ttgaatttat ctaatatgac accaattgag gcattggtga
    14701 agttaagtga attacaaaat caattaaaat agaggtgttg caaaatgggg aaaattaaag
    14761 aactccaaac ctcattagca aataaaatcg cagcaggtga agtagttgaa agaccgagtt
    14821 ctgttgtgaa agaactgttg gaaaatgcta tagatgcagg cgctacagaa ataagcattg
    14881 aagtagagga atctggcgtc caatctattc gcgtagtcga taatggaagc ggaattgaag
    14941 cggaagactt aggattagta tttcatagac atgcgactag taaattagat caagatgaag
    15001 atttatttca tattaggaca ttaggattcc gtggtgaagc actagccagt atttcatcag
    15061 ttgcaaaagt aacattgaag acttgcacgg ataatgctaa tggtaatgaa atatatgtag
    15121 aaaatggtga aatattaaat cataagcctg caaaagcgaa aaaaggaaca gatattcttg
    15181 tagaatcatt attttataat acaccagcac gtttaaaata tattaaaagt ttatatactg
    15241 aactaggtaa aataacagat attgtcaaca gaatggcaat gagccatccg gacattcgaa
    15301 tagcgctcat ttcagatggc aaaacaatgt taagtacaaa tggttcagga cgaactaatg
    15361 aagtgatggc agagatttat gggatgaaag ttgcacgaga tttagtacat atatctggag
    15421 atacaagtga ttatcacatt gaaggttttg ttgcaaagcc tgaacattct agaagtaata
    15481 agcactatat ttctattttt attaatggac gatacattaa aaactttatg ttaaataaag
    15541 cgattttaga aggctatcat acactcttaa caataggtag gttcccgatt tgttatatta
    15601 atattgaaat ggatccaatc ttagtagacg taaatgttca tccaacaaaa ctagaagtgc
    15661 gtttatcaaa agaagagcaa ctatatcaat tgatagtgag caaaatacaa gaagcattta
    15721 aagaccgtat attaattcct aaaaataact tggattatgt gccgaaaaaa aataaagtgt
    15781 tacattcatt cgaacaacaa aaaatcgaat ttgaacaaag acaaaacaca gagaataacc
    15841 aagagaagac gttttcatct gaagaaagta acagtaagcc atttatggca gaaaatcaaa
    15901 atgatgagat agttataaaa gaagattcgt ataatccatt cgtaacgaaa acgtctgaaa
    15961 gtttaatagc tgatgatgaa tcttccggtt ataataatac acgtgaaaaa gataaagact
    16021 acttcaaaaa acaacaagaa attttacaag aaatggatca aacatttgat tcaaatgatg
    16081 atacatctgt gcaaaattat gagaataaag cgtctgatga ttattatgat gtaaacgata
    16141 ttaaaggaac taaaagtaaa gaccctaaac gaagaattcc atatatggaa attgttggac
    16201 aagtacatgg aacgtatatt attgctcaaa atgaatttgg catgtacatg attgaccagc
    16261 atgcagctca agaaagaata aaatatgaat attttcgaga taaaataggt gaagttacca
    16321 atgaagtaca agatttatta atcccgttaa catttcattt ttcaaaggat gaacaattag
    16381 tcattgatca atataaaaat gagcttcaac aagtaggtat catgttagaa cattttggtg
    16441 gtcatgatta tattgtaagt agctatccag tttggttccc taaagatgaa gtagaagaaa
    16501 ttattaaaga tatgattgaa ctaattttgg aagagaaaaa agtagatatc aaaaaattac
    16561 gtgaagatgt agcaatcatg atgtcatgta aaaaatctat taaagcgaat cattatttac
    16621 aaaaacatga aatgtctgat ttaattgatc aattaagaga agcggaagat ccatttacat
    16681 gtccacatgg tcgtccaatt atcattaatt tttcaaaata cgaattagaa aaattattta
    16741 agcgtgtgat gtagagagga tgaatcaagt gaataacaac atattgcctg ccataagaaa
    16801 cattaaagat ttagagaaac tgattaaaac agattataaa atgtgtgtgc ttctagatat
    16861 gcatatagga catataaaaa gtattatgga attgctgaag caaaatcata tagagtgttt
    16921 tattcatata gatttgataa aaggtttaag ccacgatgaa tttgcaagtg aatttattat
    16981 tcagcaatac aagccaaaag gtattgtatc gactaaatct aaagtaataa aaaaagcgaa
    17041 atcattaaat actttaacga tttttagagt gtttattatt gatagtcaag cattgaaacg
    17101 cagtatagat ttgataaaaa aagttgaacc tgattttgta gaagtacttc caggtgttgc
    17161 gagtaaagcg attcatcata ttcagaaaga aacaaacaca caagtcattg caggtggcct
    17221 aattaataca atagatgaag tcaatgaagc tgttaaaaat ggagcgaaat atgtaacaac
    17281 tagttatgat aaactctggt gaatcataag tctgcaaaaa ttagattttt cactagattt
    17341 agtcatataa catattaaaa
//
LOCUS       DAHHRR010000045        17329 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.045, whole genome shotgun sequence.
ACCESSION   DAHHRR010000045 DAHHRR010000000
VERSION     DAHHRR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17329
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.045"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            140..259
                     /locus_tag="NBI42_001346"
     CDS             140..259
                     /locus_tag="NBI42_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777769.1"
                     /translation="MERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKYSFKKSS"
     gene            complement(408..725)
                     /locus_tag="NBI42_001347"
     CDS             complement(408..725)
                     /locus_tag="NBI42_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HCD1777770.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
     gene            complement(1495..2355)
                     /gene="folD"
                     /locus_tag="NBI42_001348"
     CDS             complement(1495..2355)
                     /gene="folD"
                     /locus_tag="NBI42_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCD1777771.1"
                     /translation="MVAKILDGKQIAKDYRQGLQNQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPDKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGVDS"
     gene            2556..3038
                     /gene="purE"
                     /locus_tag="NBI42_001349"
     CDS             2556..3038
                     /gene="purE"
                     /locus_tag="NBI42_001349"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCD1777772.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGIDIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSMQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            3025..4149
                     /gene="purK"
                     /locus_tag="NBI42_001350"
     CDS             3025..4149
                     /gene="purK"
                     /locus_tag="NBI42_001350"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCD1777773.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVAVLDPAEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITYFPLQENEHRNQI
                     LFKTIVPARIDKMAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            4153..4857
                     /locus_tag="NBI42_001351"
     CDS             4153..4857
                     /locus_tag="NBI42_001351"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCD1777774.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMNAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            4857..5120
                     /gene="purS"
                     /locus_tag="NBI42_001352"
     CDS             4857..5120
                     /gene="purS"
                     /locus_tag="NBI42_001352"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCD1777775.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            5122..5793
                     /gene="purQ"
                     /locus_tag="NBI42_001353"
     CDS             5122..5793
                     /gene="purQ"
                     /locus_tag="NBI42_001353"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HCD1777776.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYANNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            5786..7975
                     /gene="purL"
                     /locus_tag="NBI42_001354"
     CDS             5786..7975
                     /gene="purL"
                     /locus_tag="NBI42_001354"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCD1777777.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGEHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTDRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKNYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGV
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKRIWEGAIAQCLTTQD"
     gene            7954..9438
                     /locus_tag="NBI42_001355"
     CDS             7954..9438
                     /locus_tag="NBI42_001355"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCD1777778.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSNQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            9431..10459
                     /locus_tag="NBI42_001356"
     CDS             9431..10459
                     /locus_tag="NBI42_001356"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCD1777779.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFENRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            10462..11028
                     /gene="purN"
                     /locus_tag="NBI42_001357"
     CDS             10462..11028
                     /gene="purN"
                     /locus_tag="NBI42_001357"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCD1777780.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRQCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            11043..12521
                     /gene="purH"
                     /locus_tag="NBI42_001358"
     CDS             11043..12521
                     /gene="purH"
                     /locus_tag="NBI42_001358"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCD1777781.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            12543..13790
                     /locus_tag="NBI42_001359"
     CDS             12543..13790
                     /locus_tag="NBI42_001359"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCD1777782.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQGILDFAKQQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFEAFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPITKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKLTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVHDAQRDAYEK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(14056..14862)
                     /locus_tag="NBI42_001360"
     CDS             complement(14056..14862)
                     /locus_tag="NBI42_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD1777783.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDVIFIIVLIAIITLAYYCSITLPITGIDDVRLGRVG"
     gene            complement(14855..16255)
                     /locus_tag="NBI42_001361"
     CDS             complement(14855..16255)
                     /locus_tag="NBI42_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1777784.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGTNGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLENVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(16270..16845)
                     /locus_tag="NBI42_001362"
     CDS             complement(16270..16845)
                     /locus_tag="NBI42_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD1777785.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(16922..17022)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         6038 a         2565 c         3295 g         5431 t
ORIGIN      
        1 ttttgtgact aatagcgttt ttgaaaaaat tgcttcatat ttaaaaggga agttgagaat
       61 agctttcaat taaaggttat ttgaatgaaa ataaaaaaag tttgaaattg tctaataaat
      121 tgaaaatgat ggctagttca ttgaaaggaa gcgcaaacat tttaataagg aatttgccaa
      181 aagtaattta tcgttaaaca atgaaaagtg gaaatttgtt tgttcaaaat caaaatattc
      241 ttttaaaaag agcagttaag tttttataat ttgataggta gtcctttggt atacagaaaa
      301 aaatacatta ttagggttca agttgcagat gaagttggaa taacaaataa aatggtagca
      361 aaacatgttc gtttgctacc attttatttg cagtatagtg atgagggcta tttaacctca
      421 atatacctag tggaaatttt aattgcacca tctgaatcag tgacatgata tttaatttga
      481 tatttaccag atttagatgt atcaatttgg ccatcgactt taattttatc ggttaaatct
      541 ccatcttctt tatcaaatgc acttatgccg tttagaaggt tataatcttg acctttctta
      601 ataacgatat cattagcgcc tttaatttgt ggtgtagtat tcgtcgttgc atctgcattt
      661 aaatttggtg ttactagtgt agcagaaaca ccgagggctg ataatgactg taatagttta
      721 ttcataatgt aacctccgaa tgataagtca tttctgagtt agctttacta taccttaatt
      781 tatattattt acacatcata ctatagtcct aatcttaccc tataaagaat gaaatttaaa
      841 tataaattta taaataatga ggaaataatt attattttga aaatctgcca caattgtaaa
      901 attaataatt cagggataat ttgaaatcaa atataacttt attcgacagt ttgaacttaa
      961 atttttgtgt atcaaaataa taagaaaagg gaggcgaaaa tgataaagaa ccactaacga
     1021 ttaattatgt agtagtactt tatcatagtc acagcttctg tgttggggcc tcggcggctt
     1081 gcattgtctg tagaaattgg gaatccaatt tggcccatcc ccaacttgca cattattgta
     1141 agctgacttt tcgtcagctt ctttgttggg gccaccaccc caactcgcat tgcctgtaga
     1201 atttcttttc gaaattctct gtgttggggc ccctgactag aattgaaaac ttgttacaag
     1261 cttattttca tttaattaac tactgtctat ataaaattgt agagcctaga acaatgtgaa
     1321 atgctccctt caaagccttc attttttcaa tgtctacttt gaagggagca tttgattgat
     1381 taatgtccca gattcttctt attattcatt gcaattgcga aataatattt aatgtggatt
     1441 aaaagagttc attaaaactc agatattaat tctcagtaca taactaatgt gaatttacga
     1501 atcaacacct cgacgcattt tttctgcaag caaggtatta tttaatacca ttgtaattgt
     1561 taatggacca acgccaccag gaacaggtgt tatagctcca gcaatttctt taaccgcatc
     1621 ataatcaacg tcacctttta atttgccatt ttcatctggc gtattgccaa catcgataat
     1681 tactgctcct tctttgacaa catcttttgt tactaaacca ggcttaccaa ctgcactgac
     1741 tatgacatct gcatctttta aatatgatgc catatccttt gaacgagaat gtaagattgt
     1801 tactgatgca tttttttgaa gtagtaactt agaaactggt tgtccgacaa tatgacttcg
     1861 tccaattact actgcatttt taccttctaa atcaatatca gcatgtttta atatttccat
     1921 gatgccaagc ggtgtgcaag gtacaaaagt ttgttcatcg atatataatt tccctatatt
     1981 tattggatgg aaaccgtcca catctttatc aggattgatt gcttctaata ttttctgttc
     2041 gctaacttgt tttggtaatg gtacttgtac caaaatacca cttacagaat catcattatt
     2101 tagtctattt agttcgttta atacttcttc ttcagtagct gtttcttcca aatgtacgat
     2161 ttctgaaatc ataccaattt tttcagctgc tttctttttt gatctaacat aactttgact
     2221 agcgccatca ttaccaacta atataacgga taatttaggt gtaaaaccct tttcttttag
     2281 cgcttcaact tgattttgta acccctgtct gtagtctttg gcaatttgtt taccatctaa
     2341 aattttagca accataaaaa cttctcctcc tattgtcaat atcctaactt tgttagttca
     2401 taaaactaat ttacatagca aaagacgtaa attcaaacaa aaaatcaaaa taaagttcga
     2461 ttttcgattg aaaaagcaga aattgcttgt tatgctatat ctataatata caactaaagt
     2521 aattcacaat tcaaactttt gaaagggtgt acaaagtgaa agtagcagtc attatgggca
     2581 gttcttccga ttggaaaatt atgcaagaga gttgtaacat gttggattat tttgaaattc
     2641 cgtacgaaaa acaagtagta tccgcacatc gtacgccaaa aatgatggtt caatttgctt
     2701 ctgaagcgag agaaagaggt atagacatta tcattgcagg cgctggcggt gcggcacatt
     2761 taccaggtat ggttgcatca ttaacgacgc taccagttat tggagtgccg attgaaacaa
     2821 aaagtttaaa gggtatagat tctttattat caattgttca aatgccagga ggtattccgg
     2881 ttgcaacgac tgcaattggt gcagcaggtg ctaaaaacgc aggtatactt gcagcaagaa
     2941 tgttaagtat gcaaaatcct tctttagttg aaaaactaaa tcagtatgaa tcttcgttaa
     3001 ttcaaaaagt ggaggacatg caaaatgaac ttcaataaat taaagtttgg tgcgactatt
     3061 ggcattattg gtggtggtca gcttggaaag atgatggcac aatcagctca aaaaatgggt
     3121 tataaagtgg ctgttttgga tcctgctgaa gattgtccat gtagatacgt tgcacacgaa
     3181 tttatacaag ccaagtatga cgatgaaaag gcactcaatc aattaggaca aaaatgtgat
     3241 gtgattactt atgaatttga aaacatttca gcccaacaat taaaactatt atgcgaaaaa
     3301 tacaatattc cgcaaggtta ccaagctata cagttattac aagaccgctt aactgaaaaa
     3361 gaaacattaa aaagtgctgg taccaaggtt gtcccattca tttcagtaaa agaatctaca
     3421 gatattgaca aagcaattga aacattagga tatcctttta ttgtaaaaac tagatttggt
     3481 ggctacgatg gcaaaggtca agttttaatt aacaacgaaa aagacttaca agaaggtttt
     3541 aaattaattg aaactagtga atgcgtagct gaaaaatatt tgaatatcaa gaaagaagta
     3601 tcacttactg ttacaagagg aaacaacaat caaatcactt atttcccatt acaagaaaat
     3661 gagcatagaa atcaaatact tttcaaaaca attgttccag cgagaataga taaaatggca
     3721 gaggcgaaag agcaagttaa taaaataatc caatcgattc atttcattgg aacatttaca
     3781 gttgaatttt ttatagatag taacaaccaa ttgtatgtga acgagattgc accaaggcct
     3841 cacaattccg gacattattc aattgaggca tgtgattatt cacaatttga tactcatatt
     3901 ttagcagtta ccggacaatc attaccaaat tcaattgaat tattaaagcc tgcagtcatg
     3961 atgaacttac taggtaaaga cttagattta ttggaaaatg aatttaatga acatccagag
     4021 tggcacttac atatttatgg taagtctgag cgtaaagata gcagaaaaat ggggcatatg
     4081 actgtactaa cgaatgatgt aaaccaaact gaacaagata tgtacgctaa atttgagggg
     4141 agtaattaaa gcatgacatt attatatgaa ggaaaagcga agcgcatttt ctcaacaaat
     4201 caagaaaatg aattaagagt tgaatataaa gatgaagtta ctgctggaaa cggggctaag
     4261 aaagacacaa tggcaggtaa ggggcgatta aataatcaaa ttacttctat tatatttaaa
     4321 tatttacaag aaaatggaat agaaagtcac tttattaagc aattatctga aacagaacaa
     4381 ttagttaagc ctgtgaaaat aattccatta gaagtagttg ttcgtaatat tgctagtgga
     4441 tctattacaa agcgtttagg ttttgaaaat ggtgaagttt ttagagaacc acttgtggaa
     4501 tttttctata aaaatgatgc attaaatgat ccgttgataa cggatgacca tgttaaattg
     4561 ctcaatatag catcagatga agatattgaa atactaaaat ccaaagcatt aaagattaat
     4621 aatgtgttga aacaattaat gaatgctatg aatttaaaat tagtagattt taaaatcgaa
     4681 tttggaaaga ctgagactgg tcaaattttg ttagcggatg aaatatctcc agatacatgt
     4741 cgaatttggg ataaagctac taatgcaaac tttgataaag atgtatatag aaataacact
     4801 ggatcactga ttgaaacata tcaaatattt ttaaacaaat tggaggattt aaaataatga
     4861 aaacaattga actacatatc acattacaac cacaagtatt agatacgcaa ggacaaacgc
     4921 ttactcgagc tgtacatgac ttaggttatg cacaagtgaa tgatattcgt gtaggaaaag
     4981 tattatatat gacagtggat gaggttagtg atgaaaaggt acacaacatt attacaactc
     5041 taagtgaaaa attgtttgcg aatacagtga ttgaagaata tagctataaa gtgttagatg
     5101 atgaaaagga gaatgcataa aatgaaattt gcggttcttg tttttccagg ttcgaattgt
     5161 gatagagaca tgtttaatgc tgctattaaa agtggtgttg aagcggaata tgtagattat
     5221 agagaaacat cactaagtgg atttgatggc gtacttattc ctggtggatt ttcattcggg
     5281 gattacttaa gatctggggc aatggctagt gtagcgccga ttatttctga agttaaacgt
     5341 cttgcagctg aaggtaagcc agtattaggt gtttgtaatg gatttcaaat tttaactgaa
     5401 ataggtttat tacctggtgc attattgcat aacgattcac atttatttat tagtagaaat
     5461 gaagagttag aaatagtgaa taatcaaacg gcatttacaa atctttatga acaaggtgaa
     5521 aaagttatat atcctgtagc tcacggtgaa ggtcattatt attgtactga tgaaatatat
     5581 caacaattaa aagctaataa tcaaattatt ctgaaatatg cgaataatcc gaacggttca
     5641 tatgatgata ttgcaggaat tgttaacgaa aaaggcaatg tatgtggcat gatgccacat
     5701 cctgaaagag ctttagaaac gttgttaggt actgatagtg gtgtgaaatt atttgaagcg
     5761 atggtaaaaa gttggaggga acaacatgtc taaatttatc gaaccaagcg ttgaagaaat
     5821 taaacttgaa aaagtatatc aagatatggg attaagtgat caagaatatg aaaaagtttg
     5881 cgatatttta ggcagacaac ctaactttac agaaacaggt atcttttctg ttatgtggag
     5941 tgaacattgc tcttataaac attctaaacc gtttttaaag caatttccta cgtcaggtga
     6001 gcatgtgctt atggggccag gtgaaggagc aggggtagtc gatataggtg ataatcaagc
     6061 cgtagtattt aaagtagagt ctcacaatca tccatcagca attgaaccat atcaaggggc
     6121 tgctacaggc gttggcggaa tcattcgtga cattgtctct attggggcta gacctattaa
     6181 tttgttaaac agtcttagat ttggagaatt agataataaa caaaaccaaa gattacttaa
     6241 aggggttgta aagggtatcg gaggttatgg taactgcatt ggtattccaa ctactgccgg
     6301 tgaaatcgaa tttgatgaac gttatgatgg caatccactt gttaatgcaa tgtgtgttgg
     6361 tgttatcaat cacgacatga ttcaaaaagg cacagcaaaa ggtgtaggta attctgtcat
     6421 ttatgttggt ttgaaaactg gtcgagatgg tattcatggt gctacttttg catctgaaga
     6481 attgacggaa gaaagcgaaa gtaaacgacc ttctgtacaa atcggtgatc catttgtagg
     6541 taaaaaatta atggaagcaa cacttgaagc aattacattt gatgaattag ttggtattca
     6601 agatatgggt gctgctggtt taacatcttc atcttctgaa atggcggcaa aaggtggtag
     6661 tggtttacat ttgagattag aacaagtgcc aacacgtgag ccaggtattt ctccttatga
     6721 aatgatgctt tcagaaactc aagaacgtat gttactagtt gttgaaaaag gtactgaaca
     6781 aaaattctta gatttatttg ataagcacga attggatagt gctgttatag gtgaagttac
     6841 agatacagat cgttttgttt taacatatga tgacgaagtt tatgctgaca ttccagttga
     6901 accactagct gatgaagcac ctgtatatat tttagaagga gaagaaaaaa attataatac
     6961 ttctaaaaat gattatacac acatcgatgt taaagatact ttctttaaat tacttaagca
     7021 tccgactata gcatctaaac actatttata tgatcaatac gaccaacaag ttggtgccaa
     7081 tacgataatt aagccaggac ttcaagcatc ggtagtacgt gtggaaggca caaataaggc
     7141 aattgcttca acaattgatg gtgaagcgcg ttatgtatat aacaatccat atgaaggtgg
     7201 aaagatggta gtagctgaag cttatcgaaa tttaattgcc gtgggtgcaa caccattagc
     7261 aatgaccgat tgtttaaatt atggttctcc tgaaaagaaa gaaatctatc aacagttgat
     7321 agattctacg aaaggtatgg cagaagcatg cgacattctt aagacaccag tagtttctgg
     7381 taatgtatct ttatataacg aaacgaaagg tacttctatt ttcccaacac cagttgttgg
     7441 aatggtaggt ttgattgaaa atgtaaatta tttaaatgat tttgaacctc aagttggaga
     7501 taaattatat ttaatcggtg atactaagga cgactttggt ggtagtcaac ttgaaaagtt
     7561 aatttatggc aaagttaatc atgaatttga gtcattagat ttgagttcag aagttgaaaa
     7621 aggtgaatca atcaagacag ctattcgtga aggggtatta tcacatgttc aaacggttgg
     7681 taaaggtggc ttactgatta ccttagctaa actaagtgcg cattacggtt taggattaaa
     7741 gtcttcaata gatataacaa acgcacaatt gtttagtgag acacaaggcc gatatgttgt
     7801 ttctgttaaa tcaggtaaaa ctttaaatat tgataatgca atagaaattg gacttttaac
     7861 agatagtgat aatttcaaag taacaacacc atatacagag attagtgaaa atgtttcaga
     7921 tattaaacga atatgggaag gggcaattgc tcaatgttta actactcagg attaaacgaa
     7981 gaatgtggcg tgtttggtat ttggaatcat cctgaagcag cgcaactaac atatatggga
     8041 ctgcatagtt tgcaacatcg tggtcaagaa ggtgcaggta tagttgtttc taatcaaaat
     8101 gaattaaaag gcgagcgagg attaggctta ctaactgaag cgattaaaga tgaccaaatg
     8161 gaacgattaa aaggatatca acatgcaatt ggtcacgtcc gttatgctac ttcaggtaat
     8221 aaaggtattg aaaatattca accgtttctg tatcactttt atgatatgag tgtaggtatt
     8281 tgtcataatg gtaacctcat taatgctaaa tcattgcgtc agaatttaga aaaacaaggt
     8341 gctatcttcc attcgtcttc tgatactgaa gtcattatgc atttgatacg tcgaagtaaa
     8401 gctcctactt ttgaggaagc gttgaaagaa agtttgcgaa aagttaaagg cggttttaca
     8461 tttgcgattt taactaaaga tgcgttatat ggcgcagtag atccaaatgc tatcagacct
     8521 cttgttgtag gtaaaatgaa agatggtaca tacatccttg caagtgaaac atgtgcaata
     8581 gatgtgttag gtgcagaatt tgttcaagat attcatgcag gtgaatatgt cgtgattaac
     8641 gataaaggta ttacagttaa atcttataca catcatacga caactgcaat ttctgcgatg
     8701 gaatatattt attttgctag accagattca acaatagctg gtaaaaatgt ccatgcagta
     8761 cgtaaagctt ctggtaaaaa attagcccaa gaaagccctg taaatgctga tatggtcatc
     8821 ggtgtaccca attcatcgct atcagctgcg agtggttatg ctgaagaaat aggtttgcca
     8881 tatgaaatgg gactagttaa aaatcaatat gttgcaagaa catttattca accaactcaa
     8941 gaattacgtg agcaaggtgt gagagtgaag ttatctgcgg taaaagatat agtagatggg
     9001 aaaaatatca ttcttgttga tgattccatt gttcgaggta cgacaattcg acgcattgtg
     9061 aaaatgttaa aagattctgg tgcaaataaa gtgcatgtgc gtatagcatc accggaattt
     9121 atgttcccaa gtttttatgg aatcgatgtt tcaactacgg cagaattaat ttctgcaagc
     9181 aaatcacctg aagaaattaa agattatatt ggcgctgatt cattagcata tctatctgta
     9241 gatgggttaa ttgaatcaat tggtttagat tatgacgcgc catatagtgg cttatgtgta
     9301 gaaagtttca ctggagatta tcctgcaggg ttatatgatt atgaagcaaa ttataaagca
     9361 catttaagtc atcgacaaaa gcaatatatt tctaaaaaca aacacttttt tgatagcgag
     9421 ggaaatttaa atgtctaaag catatgaaca atctggtgta aatattcatg ctggttatga
     9481 agctgtagaa agaatgtcta gtcatgttaa acgtacgatg cgtaaagaag ttatcggtgg
     9541 tttaggtgga ttcggtgcta catttgattt atcacaatta aatatgacag caccagtttt
     9601 agtttctgga acagacggag taggtacgaa attaaaacta gctatcgact atggcaaaca
     9661 tgattcgata ggtatcgatg cagtcgcaat gtgtgttaat gatattttaa cgacaggtgc
     9721 agaaccatta tattttttag attacattgc tacaaacaaa gttgttcctg aagtcattga
     9781 acaaattgtt aaaggtatta gtgatgcatg tgttgaaacg aatactgcac ttatcggtgg
     9841 agagactgct gaaatgggtg aaatgtatca cgaaggcgaa tatgatgtag ccggatttgc
     9901 tgttggagca gttgaaaagg atgactatgt agatggttca gaagtgaaag agggacaagt
     9961 tgttatagga cttgcgtcaa gtggcattca ttcaaatgga tacagtttag tgcgcaaatt
    10021 aattaatgaa tcaggcattg atttggcatc aaacttcgag aatcgtccat ttatagatgt
    10081 ctttttagaa ccaactaaat tatatgtcaa acctgtactt gctttgaaaa aagaagtttc
    10141 tattaaggca atgaatcata ttactggtgg aggtttttat gaaaatattc cacgtgcatt
    10201 gccagccgga tatgctgcta gaattgatac tacatcattt ccaacgccaa aaatatttga
    10261 ctggttacaa caacaaggca atatagacac aaatgaaatg tataacattt ttaatatggg
    10321 tattggctat acggttatcg ttgatgaaaa agatgtatca cgcgctttga agattttagc
    10381 agaacaaaat gtggaagcct atcaaattgg tcatattgtg aaaaatgagt caactgcaat
    10441 cgaattgttg ggggtataac aatggttaaa attgcgattt ttgcatcagg ttcaggaagt
    10501 aactttgaaa atatagttga acatgttgaa tcaggaaaac ttgaaaatat tgaagttacg
    10561 gcgctatata cggatcatca aaatgcgttt tgtatagata gagcaaaaaa gcatgatatt
    10621 cctgtttata ttaatgaacc aaaacaattt gattcaaaag cagcgtatga acaacattta
    10681 gtaacattat taaatgaaga taaggtagag tggattattt tagctggcta catgcgtcta
    10741 attggtccag acttattagc ttcatttgaa ggtaaaatat tgaatataca tccatctcta
    10801 ttgccgaaat ataaggggat tgacgcaata ggccaagcat atcatagtgg cgatactatt
    10861 actggttcga cagtacatta tgttgatagt ggtatggata cgggagaaat tattgaacag
    10921 agacaatgtg atattagacc ggacgattca aaagaacaat tagaagagaa agtaaaaaaa
    10981 ttggaatatg agttatatcc aagtgttatt gctaaaattg taaaataagg agtatgtatg
    11041 tgatgaagaa agctattttg agcgtatcaa ataaaacagg tattgtagag tttgctaaag
    11101 cgttaacgca attaaattat gaattatatt caacaggtgg tactaaacgt atattagatg
    11161 aagcaaatgt accagttcgt tctgtttcag acttaacaca ttttccagaa ataatggatg
    11221 gccgtgttaa aacattacat ccggctgttc atggtggcat tttagctgat cgaaataaac
    11281 cgcagcattt aaatgaatta tcagaacaac atatagattt aattgatatg gtagtagtta
    11341 atttatatcc attccaacaa actgttgcaa accctgatgt gacgatggac gaagcaattg
    11401 aaaatattga tattggtggt ccaacaatgt tacgtgctgc agctaaaaac tataaacatg
    11461 taacaacaat tgtacatcct gcagattatc aagaagtatt gacgcgatta agaaacgatt
    11521 cgttagatga gtcatataga caatcattaa tgatcaaagt ttttgagcat actgcagaat
    11581 atgatgaagc gattgtacgt ttctttaaag gggataaaga aactttaaga tatggagaaa
    11641 atccacaaca atcagcgtat tttgtgagaa cttcgaatgc taagcacacg attgcaggcg
    11701 ctaaacaatt acatgggaaa caattaagct ataacaatat taaagatgca gatgctacac
    11761 tagctttagt taaaaagttt gatacacctg ctgcagttgc ggttaaacac atgaatccat
    11821 gtggtgttgg tatcggtgac acgatagaac aagcatttca acatgcgtat gaagcggata
    11881 gtcaatcaat atttggtgga attgttgcat taaaccgagc tgtaacacct gagttagcag
    11941 agcaattgca tagtatcttt ttggaagtta ttattgcacc aaaatttaca gatgaagcat
    12001 tagatatttt aaaacaaaag aagaatgtaa gattattaga aattgatatg actatagaca
    12061 gtaacgaaga agagtttgtt tcagtatctg gcggatattt agttcaagat aaagacaatt
    12121 atgtcgtgcc aaaagaagaa atgaaagttg ttacagaagt tgcacctact gatgaacaat
    12181 gggaagcaat gttattagga tggaaagttg taccatcagt aaaaagtaat gcaattattt
    12241 taagtaataa taaacaaact gtaggtatag gtgctggaca aatgaatcgt gtcggtgctg
    12301 ctaaaattgc gttagagaga gctattgaaa tcaatgatca tgtagcgtta gtatctgatg
    12361 gatttttccc tatgggagat acagttgaac ttgcagcaca acatggtata aaggcaatta
    12421 tccaaccggg tggttcgatt aaagatcaag attcaattga tatggctaat aaacatggta
    12481 ttgcaatggt agtcacaggc actcgacatt ttaaacacta attttaaagg ggcgtaacat
    12541 aaatgaatgt attagtaatt ggtgctggtg gacgagaaca tgcacttgca tataaactta
    12601 atcaatcgaa tctagttaaa caagtgtttg tcattccagg taatgaggca atgacaccta
    12661 tagctgaagt acacactgaa atttcagaat ctgatcatca agggatacta gattttgcta
    12721 aacagcaaaa tgttgattgg gtagttatag gtccagaaca accgctaatt gatggattag
    12781 cagacatttt aagagcgaat ggtttcaaag tgtttggtcc aaataagcaa gcagctcaaa
    12841 tcgaaggctc aaaattattt gctaaaaaga taatggaaaa atataatatt ccaactgctg
    12901 attataaaga agttgagcga aaaaaggatg ctttaacata tattgagaac tgtgaattgc
    12961 ccgttgttgt caagaaagat gggttagctg ctgggaaagg cgttattatt gcagatacta
    13021 ttgaagcagc cagaagtgct attgagatta tgtatggtga tgaagaagaa ggtactgttg
    13081 tatttgaagc gtttttagaa ggagaagagt tctcgctaat gacatttgta aatggtgatt
    13141 tagcagttcc tttcgactgt attgcacaag atcataaacg cgcatttgat catgatgaag
    13201 gaccaaatac tggtggtatg ggggcttatt gtccagtacc acatattagt gacgatgttt
    13261 taaaacttac aaatgaaacg attgcacaac ccattacaaa ggcaatgctt aatgaaggtt
    13321 atcaattctt cggtgtatta tacattggtg ctattttaac taaagatggt ccaaaagtaa
    13381 ttgaatttaa tgcccgtttt ggtgatcctg aagctcaagt attattaagt cgcatggaaa
    13441 gtgatttaat gcagcatatt attgatttag atgaaggaaa acttactgaa ttcaaatgga
    13501 aaaatgaatc tattgtaggg gtcatgttgg catcaaaagg atatcctgat gcatatgaaa
    13561 aagggcataa agtaagtggc tttgatttaa atgaaaacta ttttgttagt ggattaaaga
    13621 agcaaggtga tacctttgtt acttcaggtg gtagagttat acttgccatc ggaaaaggtg
    13681 acaatgtaca tgatgcacag cgagacgcat acgaaaaagt atctcaaata caaagtgacc
    13741 atttattcta tcgtcatgac attgcgaata aagcactaca acttaaataa gtatatttaa
    13801 aaatactaag attagctatg aacgaatcta taatgataga ttttttcata gcttttttag
    13861 ttgtagagtc taggacattg atttctgtac caaatttgtg attatgtata tgtaatacaa
    13921 aagaggcgcc acaacatgtt tggatgaaca aaataacatg tttgtggcac ctcttttgtt
    13981 tagtatggaa taaatggttt tctttttcta tacaatgaat ttctaattta gtatctatac
    14041 aattatggat aaaatttaac ctacacgacc aagacgaaca tcatctatgc ccgtgatggg
    14101 taaggtgatt gaacaataat atgctaaagt aataatggca attaaaacaa taataaagat
    14161 tacatcttta tatgagaaag gtacgttgta atagtaagta cgaggaccat ctctaaatcc
    14221 tttcgactcc atcgcaactg ataattgatg tgcttttcta atgttttggc ttaatagagg
    14281 tatgattaaa tgcttaaatc gctttaatcc tctataattt gctgcgtcta tcatctgata
    14341 gcgcattttt aaagatctgc gaagctgtat taaagaacta atcattaaag gtatcatacg
    14401 aatggcagcc atgaatgcat aagcaacttt tgatttaacc tttaaatgtt gcattaaact
    14461 ataaaaaatc atgacaacct gagatgtgag tgcgattaag ataccgaaaa atgaaatagc
    14521 aatggttctt aatgatacat gtaaaccacg aactaaactt tctgttgtaa tatggataaa
    14581 tccgaatttc aaaattgtat ggctaccgtt cccgtataaa atcatgaaca gggaagagag
    14641 taatgcaaag ccaatactta tagttataaa aattgctgta attttaaact gagtaccatt
    14701 aaacatcaat aagaaaacta acattaagat agtgatataa agcataaaat cgaaattatg
    14761 cacaaatata ataaagaaaa atagtataat tccaagaaat agtttcgtta taatgttgac
    14821 atcatcaaca aatgattgcc gaactttcca ttgctcatac attcgtatca ccatcacaat
    14881 ctagtaacgc accatctgaa attttaagtc ttcttgatgg ataacgttca attatttcat
    14941 catcatgtgt aaccatgaca atactttgtc ccaaattaat tcgtttttgg aaaagtttaa
    15001 tcaactggaa tgtattatgg ctatcaagtc caaatgtcgg ttcatctaaa aagatgatat
    15061 cagctttaga acttagtgcg gtagctacgc taaggcgtcg tttttgacca atagacaact
    15121 cataaggatg ttgatctttt acattttcta aatctaaaag ttttaaaagt tgtatcgttt
    15181 catcatcact ttgatcttta gaaaggtgat taaaatgaat gttaatttca tcataaaccg
    15241 aatttgttat aaattgtaat tccgggtttt gataaactag gtacatgtgt tttgctgcat
    15301 gtttaatttt tgttaaacgc tgattttcaa aataaacatc accttgatat ttaatcaatt
    15361 gcataattga ttcaagcaag gttgttttac cactaccatt tgtccctgta attgtaatcc
    15421 actcacctag accaatttct aaatctgaga atgagagcaa tgttgattta ccgcgaataa
    15481 tacgtccatt tttaaattgt aataagtgtg agtttgttgt tggaaagtca acacgacttg
    15541 gtgcgaattc ccatgcacgt ggatgccaca caccatattc actgagtaaa tgaacatact
    15601 tctgtaatat gatttcagga cattcatcgg caatgatatt tccgttataa tccatcaaaa
    15661 tgacgcggtc gacatgattc cagatgtgtt taactttatg ttcaacgatt acaacagttt
    15721 gatcttccca aagttcaatt agtttagtcc ataaatcttc ggttgcttga acatctaaca
    15781 ttgctgtcgg ttcatctaaa aacaatgttt ttgattgttg aagaatggtt tcaacaattg
    15841 ccaatttctg tttcatcccg ccacttaaat ctttgatata cgtttcaggg gtaacattta
    15901 aattgaccat atttaaagca ttgataatta acgcatccat gtcttcacgt ggtacttgtc
    15961 tattttctaa aacgaatgca agttcttcgt atacttttgg catacaaaac tggctatcag
    16021 ggtcttggaa aataacgcca cttaatgggt caacgattag ttcatcatat ttcataggta
    16081 attcaattaa attaggaaca ataccactta atacattcag aagtgtactt ttaccgcaac
    16141 cagaaggacc gagtaaaagt actttttctt tgtcttgaat agtgatattt aaatgatcga
    16201 aaattttacg ctgaccactt ggatatttta atcgtaaatc acttactttt aacactctga
    16261 atgctcctct tataagttgt cgtaatcttc tttagcagct ggtctaaata attttgttac
    16321 acctgtctta tctaaagctt ttactaaaag gtaagataag acgccggcga ctactgcacc
    16381 actaattaat ctaaatacta tgaataatgt taagttccaa cctgcaactt catttaaata
    16441 accatagaaa taatctatcg ggaaagccgc gattgctgta caaaaacctg ctaacatagc
    16501 taccataact gaacgagatt gatatttaaa aattgcaaag acaagttcac acgctaaacc
    16561 ttgtataaaa gcgtaaacaa ttgtcggaat gtcgaaacga cccataatga tagtttcgcc
    16621 ggcaccagca gcaaattcag ccagtaaagc aatacctggt tttggaataa ttagatagca
    16681 gacaatcgct gccatgaacc aaaccccgtt tgttaattgt tcgaggtgaa ggcctgtagc
    16741 ttgcacacca ttgtaaacaa accaccataa attgtaaata actgcgaata ctactgaaat
    16801 aagtacggtt actagtattt cagatagctt taaacctttt gacattttta catcctccta
    16861 ataaaaaaac gcacaaccat ccataggaaa gttatgcgtt cacaatatat attagtaaaa
    16921 catatgtata gtaacacttt cctacgctag ttcaagctag atcaggttca aagggtttga
    16981 gggcaagcct catctcagta taaaacaccc ctagtgtgtg cgatttattt aattaattat
    17041 actgtaagac gtttgtaaac ttatgtcaat aggttgtctt catgaaatgt cgtttaattc
    17101 gatttaaaat ttataatatt agcattggat ttaaattgaa gatgtagtag gaatgttagt
    17161 aattaaagat ataaaaatat gtgacatgta ataatattga gctgataaat gaagaggggt
    17221 acttatcaat catacctatt taacaacagt gaagaaaccg tgcataatgg cttatgaatt
    17281 atcatttcca ggtaaaagag ggatacttgc gccgagcaca agcaaaaaa
//
LOCUS       DAHHRR010000046        17270 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.046, whole genome shotgun sequence.
ACCESSION   DAHHRR010000046 DAHHRR010000000
VERSION     DAHHRR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17270
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.046"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            218..2635
                     /locus_tag="NBI42_001363"
     CDS             218..2635
                     /locus_tag="NBI42_001363"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCD1777786.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTKGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGVGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            2657..2968
                     /locus_tag="NBI42_001364"
     CDS             2657..2968
                     /locus_tag="NBI42_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD1777787.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            3294..9854
                     /gene="sasC"
                     /locus_tag="NBI42_001365"
     CDS             3294..9854
                     /gene="sasC"
                     /locus_tag="NBI42_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HCD1777788.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDTNVANNRGLANSAQNTPNQSATPNQSTNQALVNHNNGSIANQAT
                     PAPIQPSASPAQNNNHSDANSTATETVSNANNNDVVSNNTTLNVPNRTNENGSGGHLT
                     LKEIQEDVRHSSDKPELVAIAEQASNRPKKRSRRAAPADPNATPADPAAAAAGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPSVTVVDNLPGFTLIN
                     GGKVGVLSHAMVRTSMFEAGSNRTYQAQGNVLALGRISGTDASNHGDFNGIEKSLTVN
                     PNSELIFEFNTMPTKNGQGATNVIIKNGVTNDTIAEKTIEDGPTLRLFKVPDNVNNLK
                     IQFVPKNDAITDTRGIYQLKDGYKYYDFVDSIGLHSGSHVYVERRTMEPTATNNKEFT
                     VTTSLKNNGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNDTLTYKTYTQ
                     DFINSPAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNDLKRRAQT
                     ILAENRNNVPLNKRVSQADIDTLTNQMQNTLIRSVDAENAVNQKADQMEDLVNQNDEL
                     TDEEKQAAIQVIEQHKGNIIGDIGDQTTDAGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQRAIINATPDATEDEIQDALNQLTTDETDAIDNVTNATTNADVETAKNNG
                     INTIGAVVPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQQIATINANPDAT
                     QEERQAAIDKVNAAVAAANTNILNANTNADVEQVKTNAIQGIQAIQPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDARNAVNEKARETITNINATPGATREEKQEAIDRVNTLKNRALN
                     DIGVTSTTAMVNSIRDDAINQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQAGTQAINAIQPNIIKKPAALEQI
                     KQHYNAKLAEINQTPDATDDEKNAAINTLNQERQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEVVKQTPNATDEEKQAAVNRINQLKDQALNQ
                     INQDHTNDQVDATTNQMITAIDNVEADVVVKPKAIADIEKAFKEKQQQIDNSIDSTDN
                     EKEVATQALAREKEKALAAIDQAQTNNQVNQAATDGLSAIKIVQPETKVKPAAREQIN
                     QKANELRAQINQDKEATAEERQAALNKINDFVNQAMSDISNDRTNQQVTDTKTQALDN
                     IAQVNPEHIVRAAARDAVNQQYEAKKQEIEQAEHATDEEKQVALNQLTNNKERALQNI
                     NQAVTNDNVKHVETNGIATLKGVEPHIVVKPEAQQAIKASADKQVDSIKDTPDATVDE
                     LDEANQLISDTLKKAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNKAINQIN
                     HADKTAQVNQDSINAQNIISKIKPATTVKATAIQQIQNIATNKINLIKANNEATDEEQ
                     NAAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERDSILAQLQNIYDTAIGQIDQDRSNTQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSDRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALGDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKVIQSAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(9971..11239)
                     /locus_tag="NBI42_001366"
     CDS             complement(9971..11239)
                     /locus_tag="NBI42_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1777789.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSNVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIEGQHVTIKEEEAVSRIEVNTDQTFTVHTQSSSYVSHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKRISHQMDMLFTHFGISGPATLRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFEFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            11645..13306
                     /locus_tag="NBI42_001367"
     CDS             11645..13306
                     /locus_tag="NBI42_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD1777790.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNVHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            13303..13998
                     /locus_tag="NBI42_001368"
     CDS             13303..13998
                     /locus_tag="NBI42_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCD1777791.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKISVRGELIEYVENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLSDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            14015..14437
                     /locus_tag="NBI42_001369"
     CDS             14015..14437
                     /locus_tag="NBI42_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1777792.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKTEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKANPKEYANRLKNNPKAFFEEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            14929..16338
                     /gene="sapep"
                     /locus_tag="NBI42_001370"
     CDS             14929..16338
                     /gene="sapep"
                     /locus_tag="NBI42_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HCD1777793.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            16342..17190
                     /gene="dat"
                     /locus_tag="NBI42_001371"
     CDS             16342..17190
                     /gene="dat"
                     /locus_tag="NBI42_001371"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HCD1777794.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAKDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
BASE COUNT         6457 a         2595 c         3187 g         5031 t
ORIGIN      
        1 aatttcgttt ggagcttttc tacagaaagc tagtgatgct gagagctagt gttaaggact
       61 aaatgtaaat cgtattaatt ttaaattgaa tgaatgacat ctcttactat taaaatgagt
      121 gcacaatttt tgtgaaatag ggtggtaacg cggcaaatgt cgtccctatg taaatagaat
      181 agttagaggt gtcttttttt attgaatagg aggaaatgtg ttgaattaca accacaatca
      241 aattgaaaag aaatggcaag actattggga cgaaaataaa acatttaaaa caaatgataa
      301 cttaggtcaa aagaaatttt atgctttaga catgtttcca tatccatcag gtgctggatt
      361 acatgttgga catcctgagg gctatacagc aacagatatc atttcaagat ataagagaat
      421 gcaaggatat aatgtattac atccgatggg gtgggatgca ttcggattac cagcagagca
      481 atatgcttta gacactggca acgacccacg tgaatttaca aagaaaaata tccaaacttt
      541 taaacgacaa attaaagaat tagggttcag ttatgattgg gatcgtgaag ttaatacaac
      601 agatccagaa tactataaat ggacacagtg gattttcata cagttatata acaaaggttt
      661 agcatacgtt gatgaagttg cagttaactg gtgtccagca ttaggcactg ttttatctaa
      721 cgaagaagtg attgatggtg tctctgaacg tggtggacat ccagtttatc gtaagccgat
      781 gaaacaatgg gtacttaaaa tcacagaata tgcagatcaa ttattagcag atttagatga
      841 tttagattgg cctgagtctt taaaagatat gcagcgcaat tggattggac gttctgaagg
      901 ggccaaagtt tcatttgatg tagataatac gaaaggaaaa gtagaagtat ttacgactag
      961 accagataca atctatggtg catcattctt agtcttaagt cctgaacatg cattagttaa
     1021 ttcaattaca actgatgaat ataaagaaaa agtaaaagct tatcaaacag aagcttctaa
     1081 aaagtcagat ttagaacgta cagatttagc aaaagataaa tcaggtgtgt ttactggtgc
     1141 atatgcaatt aatcctttat ctggtgaaaa agtacaaatt tggattgctg attatgtatt
     1201 atcaacatat ggtactggag caattatggc agtaccagcg catgatgaca gagattatga
     1261 atttgctaaa aagtttgatt tgccaatcat tgaagtcatt gaaggtggaa atgttgaaga
     1321 agcagcatac actggtgaag gtaaacatat taattctggt gaacttgatg gtttagaaaa
     1381 tgaagcggca attactaaag ctattcaatt attagagcaa aaaggtgttg gcgaaaagaa
     1441 agttaattac aaattaagag attggttatt cagtcgtcag cgttattggg gcgaaccaat
     1501 tcctgtcatt cattgggaag atggaacaat gacaactgtt cctgaagaag agctaccatt
     1561 gttgttacct gaaacagatg aaatcaagcc atcagggact ggtgagtctc cactagctaa
     1621 tattgattca tttgtaaatg ttgtagatga aaaaacaggt atgaaaggac gtcgtgaaac
     1681 aaatacgatg ccacaatggg caggtagttg ttggtattat ttacgttaca tcgatcctaa
     1741 aaatgaaaat atgttagcag atcccgaaaa attaaaacat tggttacctg ttgatttata
     1801 tatcggtgga gtagaacatg cggttcttca cttattatat gcaagatttt ggcataaagt
     1861 cctttatgat ttgggcatcg tacctactaa agaacctttc caaaaattat ttaaccaagg
     1921 tatgatttta ggagaaggta atgagaagat gagtaaatct aaaggaaatg taatcaatcc
     1981 tgatgatata gtacagtctc atggtgcaga tactttacgt ctttacgaaa tgtttatggg
     2041 acctttagat gctgcaattg catggagtga aaaaggatta gatgggtctc gtcgattctt
     2101 agatcgcgta tggcgtttaa tggtaaatga agatggaaca ttgagttcaa aaattgttac
     2161 tacaaataat aaatctttag ataaagttta taaccaaact gttaaaaagg taacagaaga
     2221 ctttgaaaca ttaggattta atactgctat tagtcaatta atggtattta ttaatgagtg
     2281 ttataaagtt gatgaagttt ataaacctta cattgaaggt ttcgttaaaa tgttagcacc
     2341 tattgcacca catatcggtg aagaattatg gtcaaaatta ggacatgaag agtctattac
     2401 gtaccaacct tggccaactt atgacgaagc actacttgta gatgatgaag tagaaatcgt
     2461 tgttcaagtt aatggtaaat tgagagctaa aattaaaatt gctaaagata catcaaaaga
     2521 agaaatgcaa gaaattgcct tatctaatga caatgttaaa gcgagtattg aaggtaaaga
     2581 catcatgaaa gtcatcgctg ttcctcaaaa attagtcaat attgtagcta aataatgttt
     2641 taaggaggac tttaaaatga agtcaattac tacagatgaa ttaaaaaaca aacttttaga
     2701 atctaaacca gttcaaattg ttgatgttcg tactgatgaa gaaacagcaa tgggatatat
     2761 tcctaatgca aagttgattc caatggatac cattccggat aatttaaatt catttaataa
     2821 aaatgaaata tattatattg tatgtgctgg tggagttaga agcgctaaag ttgtagaata
     2881 tttagaggca aatggcattg atgccgtaaa tgtcgaaggc ggcatgcacg cttggggcga
     2941 tgaaggtttg gaaataaaaa gtatttaaag tagtgacata atttaaaata atattacatt
     3001 tgtaatgaca ccaagtaact tttcggttgc ttggtgtttt ttggtatgaa ttgttttctg
     3061 ttacaaaaca atctaaagcg ttcttgttat gttctattaa gattttaatt acaaaacgga
     3121 aactaaattg taataaaata aaactttatt ttataaaatg atgatgataa aattgagtga
     3181 acttaaaata ttgtacaaaa taatatagct ataaacataa tatgagggag cgtatatttt
     3241 tagcataatt ctaaacaaca cagcagagaa cagacaacca ggaggaaaat gaaatgaatt
     3301 tgttaaagaa aaataaatat agtattagga agtataaagt aggcatattc tctactttaa
     3361 tcggaacagt tttattactt tcaaacccaa atggtgcaca agccttaact acggataata
     3421 atgtacaaag cgatactaat caagcaacac ctgtaaattc acaagataca aatgttgcta
     3481 ataatagagg tttagcaaat agtgcacaga atacacctaa tcaatctgca acacctaatc
     3541 aatcaacgaa tcaagcattg gttaatcata ataatggtag tatagcaaat caagctacgc
     3601 cagctccaat acagcctagc gcgtcgcctg cgcaaaataa taatcattct gatgctaatt
     3661 caacagcaac tgagacagtg tcaaacgcta ataataatga tgtagtgtcg aataatacca
     3721 cattaaatgt accaaataga acaaatgaaa atggttcagg aggacatcta actttaaagg
     3781 aaattcaaga agatgttcgt cattcttcag ataaaccaga gctagttgca attgctgaac
     3841 aagcatctaa tagaccgaaa aagagaagca gacgtgcggc accggcagat cctaatgcaa
     3901 ctccagcaga tccagcggct gcagcggcag gaaacggtgg tgcaccagtt gcaattacag
     3961 cgccatacac gccgacaact gatcctaatg ccaataatgc aggacaaaat gcacctaacg
     4021 aagtgctgtc atttgatgac aatggtatta gaccaagtac gaaccgttct gtaccatcag
     4081 taacagttgt tgataattta ccaggcttta cacttatcaa tggtggtaaa gtaggtgtgc
     4141 taagccatgc gatggttaga acaagtatgt ttgaggctgg gagcaacaga acctatcagg
     4201 cacaaggcaa tgtacttgca ttaggacgta taagcgggac ggacgctagt aatcatggag
     4261 attttaatgg tattgaaaaa tctttaacag taaatccaaa ttctgaatta atctttgaat
     4321 ttaatacaat gccaactaaa aatggtcaag gtgcaacaaa tgtcattatt aaaaatggtg
     4381 ttactaacga tacgattgct gaaaagacta ttgaagacgg accaactttg cgattattca
     4441 aagtgccaga taatgtaaat aaccttaaaa tccaatttgt gcctaaaaat gatgcaatta
     4501 cagatacacg aggaatttat caactaaaag atggttacaa atattatgat tttgttgact
     4561 caatcggact tcattctggt tcgcatgtct atgttgaaag acgaacaatg gaacccacag
     4621 ctactaataa taaagaattt acagtcacaa cttcattaaa gaataatggt aattctggtg
     4681 cttctctaga tacaaatgac tttgtatatc aagttcaatt acctgaaggt gttgaatatg
     4741 tgaacaattc attgactaaa gattttccaa gtaacaattc aggcgttgat gttaatgata
     4801 tgaatgttac atatgatgca gcaaatcgtg tgataacaat taaaagtact ggaggaggta
     4861 cagcaaactc tccggcaaga cttatgcctg ataaaatact cgatttaaga tataaattac
     4921 gtgtaaataa tgtgccgaca ccaagaacag taacatttaa tgatacatta acatataaaa
     4981 cgtatacaca agattttatt aattcacctg ctgaaagtca tactgtaagt acaaatccat
     5041 atactatcga tatcatcatg aataaagatg cattacaagc cgaagttgac agacgcattc
     5101 aacaagctga ttatacattt gcatcattag atatctttaa tgatcttaaa agacgtgcac
     5161 aaacaatttt agctgaaaat cgtaacaatg taccattaaa taaaagagta tctcaagcag
     5221 atattgatac attaactaat cagatgcaaa atactttaat tcgaagtgtt gatgctgaaa
     5281 atgcagtaaa ccaaaaagct gatcaaatgg aagatttagt taatcaaaat gatgaactga
     5341 cagatgaaga aaaacaagca gcaatacaag ttatagagca acataaaggc aatattattg
     5401 gtgatattgg tgatcaaacg actgatgctg gcgttactag aatcaaagat caaggtatac
     5461 agacattaag tggggatact gcaacaccgg ttgttaaacc aaatgctaaa aaagcaatac
     5521 gtgataaagc aacgaaacaa agagcgatta tcaatgcaac accagatgct actgaagacg
     5581 agattcaaga tgcattaaat caattaacga cagatgaaac agatgctatt gataatgtta
     5641 cgaatgctac tacaaatgct gacgttgaaa cagctaaaaa taatggcatc aatactattg
     5701 gagcagttgt tcctcaagta actcataaaa aagctgcaag agatgcaatt aaccaagcaa
     5761 cagcaacgaa aagacaacaa ataaatagta atagagaagc aactcaggaa gagaaaaatg
     5821 cagcattgaa cgaattaact caagcaacca accatgcttt agaacaaatc aatcaagcaa
     5881 caacaaatgc tgatgttgat aatgcaaaag gtgatggtct gaatgccatt aatccaattg
     5941 ctccggtaac agttgttaag caagctgcaa gggatgctgt ttcacacgat gcgcaacaac
     6001 aaattgcaac aattaatgca aatcctgatg cgactcaaga agaaagacaa gcagcgattg
     6061 acaaagttaa cgcagctgta gcagcagcaa atacaaacat tttaaatgcc aatacaaatg
     6121 cagatgttga gcaagtaaag acaaatgcga ttcaaggaat acaggcgatt caaccagcta
     6181 caaaggttaa aactgatgca aaaaatgcca tcgataaaag tgcggaaacg caacataata
     6241 cgatctttaa taataatgat gcgacgctcg aagaacaaca agcagcacaa caattacttg
     6301 atcaagctgt agccacagcg aagcaaaata ttaatgcagc agatacgaat caagaagttg
     6361 cacaagcaaa agatcagggc acacaaaata tagttgtgat tcaaccggca acacaagtta
     6421 aaacggatgc tcgtaatgct gtaaatgaaa aggcgagaga aacaattaca aatattaatg
     6481 caacaccagg tgcaacaaga gaagaaaagc aagaagcaat agatcgtgtt aataccctta
     6541 aaaatagagc gttaaatgat atcggtgtta cttctacaac agcgatggtt aatagtatta
     6601 gagatgacgc aataaatcaa attggtgcgg ttcaaccaca tgtaacgaaa aagcaaacag
     6661 ctacaggtgt attaaatgat ttagcaactg ctaaaaagca agaaattaat caaaatacaa
     6721 atgcaacaac tgaagaaaag caagtggctt taaatcaagt ggatcaagag ttagcaacag
     6781 caattaataa cattaatcaa gcagatacaa atgcagaagt agatcaggcg caacaagcag
     6841 ggacacaagc gattaatgct atacagccca atatcataaa aaaacctgca gcattagaac
     6901 aaattaaaca acattataat gctaaattag ctgaaatcaa tcaaacacca gatgctacag
     6961 atgatgagaa aaatgcagca attaatactt taaatcaaga gagacaacaa gctattgaaa
     7021 gtattaaaca agctaacaca aatgcggaag tagaccaagc tgcgacagtg gcagagaata
     7081 atatcgatgc tgttcaagtt gacgtagtaa aaaaacaagc agcgcgagat aaaatcactg
     7141 ctgaagtggc gaagcgtatt gaagtggtta aacaaacacc taatgcaact gatgaagaaa
     7201 agcaggctgc tgttaatcga atcaatcaac ttaaagatca agcgctcaat caaattaatc
     7261 aagatcatac aaatgatcaa gtagatgcta ctacaaacca aatgataact gctatagata
     7321 atgttgaagc agatgttgtt gtaaaaccaa aagcaattgc cgatattgaa aaagctttta
     7381 aagaaaaaca acaacaaatt gataatagta ttgattctac tgataacgaa aaagaagttg
     7441 ctactcaagc acttgctagg gaaaaagaaa aagctctagc agcaattgat caagcacaaa
     7501 ctaataatca agtaaatcaa gcagcaactg atggattatc tgcaattaaa attgtacaac
     7561 ctgaaactaa ggtaaaacca gccgcacgtg aacaaattaa tcaaaaagct aatgaactac
     7621 gtgcgcaaat taatcaagat aaagaagcga cagcagaaga aagacaagct gcacttaata
     7681 aaattaatga cttcgttaat caagctatgt ctgatattag taatgataga acaaatcaac
     7741 aagttactga tacaaaaact caagcacttg ataacattgc tcaagtaaat cctgaacata
     7801 ttgttcgagc ggcagcaaga gatgcagtta atcagcaata cgaagctaaa aaacaagaaa
     7861 ttgagcaagc agaacatgcg acggatgagg aaaaacaagt agctttaaat caattaacta
     7921 ataataaaga acgtgcatta caaaatatta atcaagctgt aactaatgat aatgtaaaac
     7981 atgttgaaac aaatggtatt gctactttaa aaggtgtaga accgcatatt gtggttaaac
     8041 cagaagcaca acaggcaata aaggcgagtg cagataaaca agtagattct ataaaagaca
     8101 cgcctgatgc aacagttgat gaattagatg aagcgaatca attaattagc gacacactca
     8161 aaaaagcgca acaagaaata gaaaatacaa atcaagatgc tgctgttact gatgttagaa
     8221 atcaaacaat caaggcaata gagcaaataa aacctaaagt aagacgtaaa cgagctgcgc
     8281 ttgatagcat tgaagaaaat aataaaaatc aactcgatgc aatccgaaat acgttggata
     8341 ctactcaaga tgaaagagat gttgctattg atactttaaa taaaattgta aatacaatta
     8401 aaaatgacat tgcacaaaac aaaacgaatg cagaagtgga tcgaactgag actgatggca
     8461 acgacaacat caaagtgatt ttacctaaag ttcaagttaa accagcagcg cgtcaatctg
     8521 ttggtgtaaa agccgaagct caaaatgcac tgatcgatca aagcgattta tcaactgaag
     8581 aagaaagact agctgctaaa catttagttg aacaagcact taataaagct atcaatcaaa
     8641 tcaaccatgc tgataaaaca gcgcaagtta atcaagatag tataaatgct caaaatatta
     8701 tttcaaaaat taaaccagcg acaacagtta aagcaacagc aatacaacaa attcaaaata
     8761 tcgctacaaa taaaattaat ttaattaaag caaataacga agcgacagat gaagaacaaa
     8821 atgctgcaat tgcccaagtt gaaaaagagt taattaaagc taaacaacaa attgctagtg
     8881 cagtgactaa tgctgatgtg gcatatttat tgcatgatga gaaaaacgaa attcgtgaaa
     8941 tcgaacctgt tattaacaga aaggcgtctg ctcgagaaca attgacaaca ttattcaacg
     9001 ataaaaaaca agcaattgaa gcgaatattc aagcaacggt agaagaaaga gatagtatat
     9061 tagcacagtt acaaaatatt tatgacactg ctattggaca aattgatcaa gatcgtagca
     9121 atacacaagt tgataaaaca gcatcattaa atctacaaac aatacatgat ttagatgtac
     9181 atcctattaa aaagccagat gctgaaaaaa cgattaatga tgatcttgca cgcgtcactg
     9241 ctttagtgca aaattatcga aaagtaagtg atcgtaataa ggctgatgca ttaaaagcta
     9301 taactgcttt aaaattacaa atggatgaag aattaaaaac agcacgcact aatgctgatg
     9361 ttgatgcagt tttaaaacga tttaatgttg cattaggcga tatagaagca gtaattactg
     9421 aaaaagaaaa tagcttactg cgcattgata acattgctca acaaacatat gcgaaattca
     9481 aagcgatcgc aacaccagaa caattagcta aagtaaaagt attaattgat caatatgttg
     9541 cagatggcaa tagaatgatt gatgaagatg cgacattaaa tgacatcaaa caacatacgc
     9601 aattcattgt tgatgaaatt ttagcaatta aattaccagc tgaagcgacg aaagtatcac
     9661 caaaagtaat tcagtcagct ccaaaagttt gtacgcctat taaaaaagaa gagacacatg
     9721 aatcgcgcaa agttgaaaaa gaacttccaa atacaggttc tgaaggaatg gatttaccat
     9781 tgaaagaatt tgcactgatt acaggtgcgg ctttgttagc tagaagacgt actaaaaacg
     9841 aaaaagaatc ataattaacg aaatgacctt aatataacga cactttttag tgactcagtt
     9901 atattaaggt catttttaat tgaatgaaaa agttgatcta ctaaagagaa tcgaaccaaa
     9961 ttttatacta ttattccatt gatgcatgtg agtaatgacc ggcatataat ccagcgacat
    10021 gtcctgttac gagtgcactt gtaatattat aaccaccagt ataaccatgt atatctaata
    10081 cttcaccaca taaaaataat cccggaacta atttagacat cattgtttta ggttgaattt
    10141 ctttaagtga cacaccaccg cctgtgacaa atgccttatc tataggtaat gtcccgttta
    10201 ccttaaattc aaacccttta aacatattta ctaagtcgtt caattgttga tttgataagt
    10261 gatgtgatgt ggtattttca tcgattccag cttgttccag catgaacagt aagtagcgct
    10321 cttcaattag tccatgcaag ctgtttttaa tgattttatc tggtgtgtcc gataataatg
    10381 atgtgatgtg ttgttttaat tgttcatggt ttaattcagg aaatgcatcg attgccatag
    10441 aaatatgctg tgtcttttga tttttttgtt ctttataaac aaactgacta catcttaatg
    10501 tagctggacc actgatacca aaatgagtaa ataacatatc catttgatga ctgatgcgtt
    10561 ttttaccatt tttcttaagt actgacaatt caacatcttt taaacttaaa ccttttagac
    10621 gatttgattt gatgaaaggt tcagctgatg taattggaac ttcggtcggg aataattctg
    10681 taatggtatg acctaaatct tgtgcgaact tataaccatc accagttgaa cctgtttgag
    10741 ggacacttgt accacctgta gcaatcacaa gtgaatgact tacatagctg ctgctttgag
    10801 tatgtacagt gaaagtttgg tctgtattaa cttcgattct actaacagct tcttcttctt
    10861 taatcgttac atgttggcct tcgatagttg tcactaatgt atcaaccacg tcttgtgctt
    10921 tgttggagac tggaaacata cgcccgtgat cttcttcttt taatttaaca cccctagact
    10981 caaaaaaatc tatgatggat tcattatcaa aaattgaaaa gggactatat aaaaatttcc
    11041 catttccagg aatgttctta ataatttcag catatggtaa tcgattagtt acgttacatc
    11101 taccgccacc agatattttg agtttacgac ctagaccttt ctttttttca atgagtaata
    11161 cattgctact ttgttcgctt gcagctactg ccgccattaa gccgctaggt ccgcctccga
    11221 taataattgt ttgatacata tacgcttcct ccaaatttgt tactggacat tcaattgcta
    11281 ttttatagta cttttttaaa ctttgctata tctcttgtgt tatgagatga caattatgtt
    11341 gaatatattt ataatgtgaa cttcataaaa aatgaagatg tatgtgtatg ataagaatga
    11401 ttttctactg ttatgcgatg ttcatctcaa atagtagtat aatgtttata tgaaatttcg
    11461 taatattcta atcaaaatcg agtattcaat gacaatgcgt tgataatttt gttatgataa
    11521 ttcattgaat atacgacaat gaaataaagt tttgtgatat attcataata atgaaaaatg
    11581 attttacaga gaaatagctt ttagtttaca aaactttaat aaacatgaga tagggagatt
    11641 cgtaatgagt gaaagtaaag aaatggtgcg tggaaccttt ttaattacca ttagtatatt
    11701 aattaccaag gtattgggcg tactttttat cattccattc aactatttaa ttggtggaca
    11761 agaaaatatg gcgccgttca catacgctta tgcaccatat aatattgcaa ttgcagttgc
    11821 gacagcaggt gtaccattag cagcttcgaa atatgttgcg aaatataatg ctattggggc
    11881 atataaagtc agtcagaaat tttataaatc gagttttatt gtaatgagta ttaccggtgt
    11941 tttaggtttc ttggtactgt atttcctagc accttatatt tctgaattaa cattagctag
    12001 aaacgttcat gataaaaacg gttggtcagt cgatgatatt acttggatta taagaatcat
    12061 tagtatggtt gttatcttta ttcctgtatt agcgacatgg agaggtatat tccaaggtta
    12121 taaatctatg gggccaacag cagtgtcaga agtaactgag caaattgcac gcgtgatttt
    12181 catattaatc ggaagttatt tagtacttaa tgtctttgat ggttctattt tattagccaa
    12241 tggtattgct acttttgcag cagctgttgg tgcgattatt ggtatattca cactctggta
    12301 ttactggaga aagcgtaagc ataatattga tcgtatggtc gaatctgatt acacagatat
    12361 agatgtgtca tatggcaaga tgtataaaga aattattgct tatagtattc cgttcgttat
    12421 tgtaagtttg aattatccat tatttaactt agtagatcaa tttacacata acggtgcttt
    12481 atctttagtt ggtatacctt cacagttaca agatatattc tttaatatgc taaatatgtc
    12541 aactaataaa attgtaatga ttccgacatc attaagtgct ggctttgcag taagtttaat
    12601 tccttatatt acaaaaacat ttgcagaagg tcgcttacat gaaatgcatc atcaaattag
    12661 aacatctatt ggtgtattaa tgtttataac tgttccagct agtattggaa ttatggcctt
    12721 ggcacaacca ttattcacag ttttctatgg ttacgatcca attgttttag gccatgaccc
    12781 taaccatgat ggtagtcgct tattattcta ttatgcgcct gtagcaatac taatttcact
    12841 attaagtgta acggcatcaa tgttacaagg tatagataaa cagaaattaa cagtttatgt
    12901 tattttagct tctgtagtta tcaagttagc gttaaactat ccattaatta tgttattcca
    12961 tacacctggt gcaattttaa gtactagtat cgcattatta tttgcaattg gatgtaattt
    13021 ctatattctt aaaaaatatg cgaagtttaa attcagctat agttggattc attttgcaaa
    13081 aatattcttg tattcattta ttatgatgct aggtgtagaa ttagtattct tccttgcaaa
    13141 tctattctta gaacctacta aacttggtta cttaatcatt attattttag gtgttacagt
    13201 aggtattcta atttacggta caatcacaat taaaacgcgt cttgcggacg aatttttagg
    13261 tgaaattcct gaaaaattaa gacgtagagt taggttttta cgatgagaat agataaattt
    13321 ttagcaaaca tgggcgtcgg tacacgtaat gaggtcaaac agttacttaa aaaaggcctt
    13381 gtaaatgtca acgaacaagt aattaaatca ccaaaaacac atatagaacc agaaaacgat
    13441 aagatatcag ttcgtggaga attaatagaa tacgttgaaa atgtctatat tatgttaaat
    13501 aagccaaaag gttatatatc ggcaactgaa gatcatcatt caaaaaccgt tattgattta
    13561 atacctgaat accaacatct aaatattttt ccggttggtc gtcttgataa agatacagaa
    13621 gggcttttat tgataacgaa tgatggtgat tttaatcatg aattaatgag tccaaacaaa
    13681 catgtttcta aaaaatatga agttatttca gcaaatccta tcactgaaga cgacattcaa
    13741 gcttttaaag agggtgtaac actgtctgat ggcaaggtta agcctgcaat attaacttat
    13801 attgataatc aaacgtcaca cgtaacaatt tatgaaggaa aatatcatca agttaaacga
    13861 atgtttcata gcattcaaaa tgaggtatta catttaagac gtatcaaaat tgctgattta
    13921 gaattggaca gcaacttaga ttcaggagaa tatcgtttat taactgaaaa tgattttgac
    13981 aaattaaatt ataaataaaa gggagacgat tgatatggca aaaggtaatt tatttaaagc
    14041 gattttaggt ataggtggcg ctgtagcagc tgtacttgtt acacgtaaag atagtcgtga
    14101 caagctgaaa acagaatata ataaatataa acaagatcct caaagctata aagataatgc
    14161 taaggataaa gcgacgcaat taggaacaat tgctaatgaa acaattaaag aagtaaaagc
    14221 aaatccgaaa gaatatgcta atagattaaa aaataatcca aaagcatttt tcgaagaaga
    14281 aaaatcaaaa tttaccgaat atgacaataa gactgacgaa agtattgaaa aaggtaaatt
    14341 tgatgatgaa ggtggcgcag caccaaataa taatttacgt atcgtcactg aagaagattt
    14401 aaaaaagaat aaaaatgcat tatctgataa agaataagat gtgagtagga cagaaatgat
    14461 aaagaaccac tatgatttat tttatagtgg ttctttatcg tcagtcatag ctaatatgta
    14521 tttagaaaga agaatacatg agtgaaactc atgcataaga aatactaata tctaaagaaa
    14581 gcgtatttct ttatcttttc gaaattctct atgttgggtc cttaccccaa cttgcacatt
    14641 attgaaagct gactattggc cagcttctgt gttggggccc cggggcgcat tttcgttcgg
    14701 ttaattactg tcaatattaa attgtgtgcc tagtacattg acttatatct cgagctcttg
    14761 atttaaatat ataaattaaa aaaggaacaa agcagctatt aatgatttgt gtcatatcga
    14821 tagcatcttt ttgttccttt ttattatttt ctgacatctt attgtagttt ttatatcatt
    14881 tcatgtaaaa tagagtttaa cttatagaaa aaaggaagtc gatttcgaat gtggaaagaa
    14941 aaagttcaac aatacgaaga tcaaatcatt aatgacttaa aaggattatt agcaattgaa
    15001 agtgtgagag atgatgcaaa agcatcagaa gacgcaccag ttggtccagg tcctcgtaag
    15061 gcattagact acatgtatga aattgcacat agagatggat ttacaacaca tgatgtggat
    15121 catattgcag gaagaattga ggcaggtaaa ggaaatgacg tattaggtat cttatgtcat
    15181 gttgacgttg ttcctgctgg tgatggatgg gatagtaatc cgtttgaacc ggtcgtaaca
    15241 gaagatgcta tcatagctag aggtacactt gatgacaaag gtccaacaat tgctgcttat
    15301 tatgcaatta agatattaga agatatgaat gtggattgga agaaacgtat tcatatgatt
    15361 attggtacgg atgaagaatc tgattggaaa tgtacggatc gctattttaa aacagaagaa
    15421 atgccaacat taggttttgc accagatgca gaatttccgt gtattcatgg tgaaaaaggc
    15481 attacaacat ttgacttagt tcaaaataaa cttactgaag atcaagatga acctgattat
    15541 gaattaataa cttttaaatc tggtgaacgt tacaacatgg tgcctgatca tgcagaagca
    15601 agagtgcttg ttaaagaaaa tatgacagat gttattcaag actttgagta ctttttagaa
    15661 caaaatcatt tacaaggtga tagtactgtt gatagtggca ttctagtttt aacagttgaa
    15721 ggtaaagcgg ttcatggtat ggatccatct atcggtgtga atgcgggtct ttacttacta
    15781 aaattcttag catcattaaa tcttgataat aatgcacaag cgtttgtagc atttagtaat
    15841 cgctacttat ttaattcaga ttttggtgaa aagatgggaa tgaaattcca tacagatgtc
    15901 atgggtgatg tgacaactaa cattggtgtt attacatatg ataatgaaaa tgcgggtctt
    15961 ttcggtatca acttacgcta tccagaagga tttgaatttg aaaaagctat ggatcgtttt
    16021 gcaaatgaga ttcaacaata tggctttgaa gtgaaattag gtaaagtcca accaccacat
    16081 tatgttgata aaaatgatcc gtttgtacaa aagttagtta ctgcatatag aaatcaaaca
    16141 aatgatatga ctgaacctta tactataggt ggcggtactt atgcgagaaa cttagataag
    16201 ggtgtagcat ttggcgcaat gtttagtgat tctgaagatt taatgcatca gaaaaatgaa
    16261 tatatcacta aaaaacagtt atttaacgca actagtattt acttagaagc aatttattca
    16321 ttatgcgtgg aggaataata tatggaaaaa atttttttaa atggtgagtt tgtaagtcca
    16381 agtgaagcaa aggtttcata caacgacaga ggatacgtat ttggcgatgg tatttatgaa
    16441 tacattcgcg tatataatgg taagttattt acagtaacag aacattacga aagattttta
    16501 cgtagtgcca atgagattgg tttagattta aattattctg tagaagaatt aattgaacta
    16561 tctcgtaaat tagttgatat gaatcaaatt gaaactgggg caatttatat tcaagcaacg
    16621 cgtggtgtag cagaaagaaa tcatagcttc ccgacacctg aagtagaacc agcaattgtt
    16681 gcttatacaa agagttatga tcgtccttat gatcatttag aaaatggtgt gaatggtgtt
    16741 accgttgaag atatccgatg gttacgttgc gacattaaaa gcttgaactt attaggaaat
    16801 gtattagcaa aagaatatgc tgtgaaatat aatgcagttg aagcaattca acatcgaggc
    16861 gaaactgtca ctgaaggatc ttcaagtaat gcttatgcaa ttaaagacgg tgtgatttat
    16921 acacatccga ttaacaacta tattcttaat ggtattacac gaattgtaat taaaaaaatt
    16981 gccaaagact ataatatccc atttaaagaa gaaacgttta ctgtagattt cttgaaaaac
    17041 gcagatgaag ttattgtttc aagtacttca gctgaggtta cacctgttat taaattagat
    17101 ggtgaaccag ttaatgatgg taaagttggc ccaattacac gtcaactaca agaaggattt
    17161 gaaaagtata tagagtcaca cagtatttaa aattctttca tcatattttt agattaaatt
    17221 acttaaattt atcaactttc gtaagtgaat tatgttataa tttaaaacga
//
LOCUS       DAHHRR010000047        17123 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.047, whole genome shotgun sequence.
ACCESSION   DAHHRR010000047 DAHHRR010000000
VERSION     DAHHRR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17123
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.047"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(49..903)
                     /locus_tag="NBI42_001372"
     CDS             complement(49..903)
                     /locus_tag="NBI42_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCD1777795.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(1179..1403)
                     /locus_tag="NBI42_001373"
     CDS             complement(1179..1403)
                     /locus_tag="NBI42_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777796.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            1602..2072
                     /gene="sigS"
                     /locus_tag="NBI42_001374"
     CDS             1602..2072
                     /gene="sigS"
                     /locus_tag="NBI42_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HCD1777797.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNHRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKTSVKRKCQHNFN"
     gene            2185..2628
                     /locus_tag="NBI42_001375"
     CDS             2185..2628
                     /locus_tag="NBI42_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCD1777798.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILEVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINAKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(2615..3058)
                     /locus_tag="NBI42_001376"
     CDS             complement(2615..3058)
                     /locus_tag="NBI42_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777799.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQHVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMIVSIYFIKIVTSYFTKKLLEIKFNSK"
     gene            3356..3991
                     /gene="sdpA"
                     /locus_tag="NBI42_001377"
     CDS             3356..3991
                     /gene="sdpA"
                     /locus_tag="NBI42_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HCD1777800.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVYL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQEIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWR"
     gene            complement(4432..5145)
                     /locus_tag="NBI42_001378"
     CDS             complement(4432..5145)
                     /locus_tag="NBI42_001378"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCD1777801.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            5402..5704
                     /locus_tag="NBI42_001379"
     CDS             5402..5704
                     /locus_tag="NBI42_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777802.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            5960..6325
                     /gene="crcB"
                     /locus_tag="NBI42_001380"
     CDS             5960..6325
                     /gene="crcB"
                     /locus_tag="NBI42_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCD1777803.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLVANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAITS
                     YVFGILLCYVGIKLGGGLS"
     gene            6322..6675
                     /locus_tag="NBI42_001381"
     CDS             6322..6675
                     /locus_tag="NBI42_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HCD1777804.1"
                     /translation="MISIILVMIGGGFGAISRSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
     gene            complement(6924..7757)
                     /locus_tag="NBI42_001382"
     CDS             complement(6924..7757)
                     /locus_tag="NBI42_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1777805.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNKVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTKHKLKLYLAAQHIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHGVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKKFE
                     G"
     gene            complement(7969..8877)
                     /locus_tag="NBI42_001383"
     CDS             complement(7969..8877)
                     /locus_tag="NBI42_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCD1777806.1"
                     /translation="MDLSSPVVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKHLGAKYTPTSILYFNPKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            complement(9002..10195)
                     /locus_tag="NBI42_001384"
     CDS             complement(9002..10195)
                     /locus_tag="NBI42_001384"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCD1777807.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     regulatory      complement(10301..10411)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            10566..12158
                     /gene="pckA"
                     /locus_tag="NBI42_001385"
     CDS             10566..12158
                     /gene="pckA"
                     /locus_tag="NBI42_001385"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCD1777808.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYRKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     gene            complement(12408..13154)
                     /locus_tag="NBI42_001386"
     CDS             complement(12408..13154)
                     /locus_tag="NBI42_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCD1777809.1"
                     /translation="MPIESFTHQFEEITYLSDDLQVKALMMTPRHEVKRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHNKYP
                     QAFIHMVGFSRGGLQGLLTFQDLPVSSYIIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGVTHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVELHS"
     gene            complement(13159..13632)
                     /gene="ytkD"
                     /locus_tag="NBI42_001387"
     CDS             complement(13159..13632)
                     /gene="ytkD"
                     /locus_tag="NBI42_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HCD1777810.1"
                     /translation="MKFRDKDNRQVILTFKKDNEVADGNHVLAIPTFKNQLLFTKHNI
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIHYIAQYTIETHDQIDFVKDVYFI
                     EVKSLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            13698..13955
                     /gene="yidD"
                     /locus_tag="NBI42_001388"
     CDS             13698..13955
                     /gene="yidD"
                     /locus_tag="NBI42_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCD1777811.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(13952..14953)
                     /gene="menC"
                     /locus_tag="NBI42_001389"
     CDS             complement(13952..14953)
                     /gene="menC"
                     /locus_tag="NBI42_001389"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCD1777812.1"
                     /translation="MKLTALHFYKYSEQFKSQIVTPKVTLTHRDCLFIEIIDDKGYSY
                     FGECNAFQTDWYNFETIDSVKEALQQWFIGIKNKSFESYEAALELLAPLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREHILYIEEPFKDISMLDEVVDGTIPPIAL
                     DEKATSLSDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGVKVVIGGMYEYG
                     LSRYFTAMLARKGDYSGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDLTQLQPY"
     gene            complement(14958..16436)
                     /gene="menE"
                     /locus_tag="NBI42_001390"
     CDS             complement(14958..16436)
                     /gene="menE"
                     /locus_tag="NBI42_001390"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCD1777813.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYHNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSVILIHACWLANIEIAMINTRLTPNEMKNQMRSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGTDITMNGLLDNTMDIQYDTSNETVVPKDSLPNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYDLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHVRPDTVGMPSANV
                     DVKVKNPNKEGHGELMIKGANVMNGYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGHVPKLYFVSESNISK
                     AQLIAYLSQHLAKYKVPKYFEKVDTLPYTSTGKLQRNKLNRG"
     gene            complement(16596..17051)
                     /locus_tag="NBI42_001391"
     CDS             complement(16596..17051)
                     /locus_tag="NBI42_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCD1777814.1"
                     /translation="MKICLMLYLLIFLSSCGTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTVKYFVDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYV
                     IENLDDEVFHLSALQDINRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
BASE COUNT         5486 a         3111 c         2409 g         6117 t
ORIGIN      
        1 gctcttttta tacttgagca agtcatatca taacttaact ctatcaaatt acttattcaa
       61 atgtttactg tcatctttat acacaaagta tttgaagtat tttccttcag tcttcatgtt
      121 cttataaaag tcagcgataa ttgttgcatt actttctgcc cacttaatat ctgtagcata
      181 ttgatgttct cctggatttt ttggattcca tctcatacta tacaatgtat tttgatctgt
      241 gcttgataag aagtgcttat gaatgaaatc agcaccgcct gaaatagctt tttcaggtgt
      301 atcccaacca tgctttttag catattctgc acctgtttta attgggtctt tatcaagggc
      361 tcctactcca tagaaattgt agtatttttt gccatcaatt tcgactccat tagctaattc
      421 acttttaact gcgccagttt ctaataatgc atgtgaaatt aaataaactt cgttaacgtg
      481 cttatcttta gcagctttta agaaatcatc cgtatgtttc aataacgttg gtctatctac
      541 taacatacgt ttaattctat ttttatcaat cccttgatac tttgataaat ctaaaaattg
      601 atatttttgc ttttcattat cgataaaagt accgctatcc attgcacttt taatttcagt
      661 tgcagatgca tctctccatg catcattctt tttatttgat acctgttgac tcgtataatt
      721 atttatttgt ttctttgctg catcgtttaa tgtaacattt aacttttcaa tcttaatgtc
      781 tgatttaaca tgtttgaaaa atatctgatc ggatatcatt gagaaaaata aaaatgagac
      841 aacagcaaat atgacaacaa gtcctattat tccaaaaata gaacctttct tatgtttatt
      901 catatccaca cctcttaggt cattgttgtt atacattcag tttgactacg ataataaagt
      961 ttaacattat taaaaccgtt taacaatttt atctatgcat tgttacatat tcataaataa
     1021 acagtaatgt aaatgaaacg ttttaaacga tttatcaaca ttactttacg ttgatccacg
     1081 aatcataaat gttttatctt tttgtataac ctttgaaaac ctttatatca ataatcttat
     1141 tattcacgta tttaaaaaca aaaaattctc ttgttcgttt acttagtttc ttttttataa
     1201 tccatcataa taaaacttgc tgcattagat ctatttttat aatagtactg atttaaatca
     1261 atcgtacctt caatttgacc atcacgatac atcatttcat tagtgaagtt atttatcatc
     1321 acgaaatcag cgcgatctct aataacatct aaatcgtcgt ttcgtgcaaa aaaatgttct
     1381 aaatttaaat gtgcgtaatc cattgttacc tcccgattac aaattttgtt atatctacta
     1441 tttagcaaag taataataca tcatatcaca ttaaattaat tacttatgtc ttatatttaa
     1501 cacttaattt agcgattacg caaatgaatg atttttctaa atattttatt ctaactttat
     1561 ccggaaatat ttgttatacg tataacttgg agaatgacta cttgaaattt aatgacgtat
     1621 acaacaaaca ccacaaaatc atacaccatc ttttaaaaaa atataatatt agctataatt
     1681 atgatgagta ttatcaacta ctcttgataa aaatgtggca attgagtcag atatataaac
     1741 cctcaagcaa gcaatcttta tcctcttttt tattcactcg attaaatttt taccttatcg
     1801 atttattcag acaacaaaat caattaaaag atgtcatttt atgtgagaat aattcaccaa
     1861 cattaactga acaaccaaca tactttaatg aacatgacct tcgtttacaa gatatcttca
     1921 agcttttaaa tcatagagaa agactatggc tcaaactata ccttgaagga tacaagcaat
     1981 ttgaaattgc tgaaatcatg tcattatcgc tttcaacgat taaattaatt aagacatccg
     2041 ttaagcgtaa atgccaacat aattttaatt agtttattgt gaaatttcgg ccgatgtgtt
     2101 aaggatgttg aaacacccta acatcttcat ataaatttat tttaaccatt aaattaacaa
     2161 cttattatac gaggagtact atatttgcaa gacaattcta ctaaatatct actttatatc
     2221 caaactgcta cttcaaacca tctcgaaaca aattgtgtct ttttacattg cgattacatt
     2281 cttgaagttc caattaacaa actcgtttca tattatgcga aattgcattt atcatcacaa
     2341 agtgtgctaa ttgagactgc aaaaaacata ctaaatatta ataaactggt tcccatttac
     2401 atcaacgcaa aaactatact ttttccgtta aaacataaac gtgcaccgat acaaatttat
     2461 atcaatgcac attatattgt tggtatgact gccatagaga attcaacatt aatacatttt
     2521 caagaaggca ttcagctaga agttgatgag ccattttctc tagtttcaaa aaaatgtcat
     2581 gaaagtttag ctttgaagca cttcattgaa aatactattt cgaattaaat ttaatttcta
     2641 aaagtttctt agtaaagtat gatgtcacaa tttttataaa atatatgcta acaatcatta
     2701 ataatatcgc tccaataatt gttaaaataa caacagttgt tgataaatct accttaggac
     2761 taacgaaaaa ttgtacgcca gatagacttg taattagccc aaataaaaac aagcctaata
     2821 aagcaacata agcaaacatc atcactacta atgtaacaaa catactataa ctttttgttt
     2881 ctttatttcg aaatacatgt tgtcttaagt aagcattagc aaaggtaaca ggcttattta
     2941 agttaacgct gtcatcatca gatatagaag acaatcgttg atctaatgct tgtttctcct
     3001 tcttgttcaa aaaccataat tgctttttaa cttttttatc aaatgatgct ttattcataa
     3061 ttaaggattt ctctccttta taatattgac gtaattttaa acattagcgt caattgaaag
     3121 tgtattttat atattttaca gaaatatatg tatcaaatct acaaaaatcc atattacaat
     3181 ttcatttatg atgatttgac gctaaaatac agtctgttaa ttttttcaaa ctaatgcttt
     3241 ttaggtaagt acaatgataa attttaatcg tatatatgtt tcatatataa tgaatctagc
     3301 cataatcata ttgccttcaa aattaatctt aaaaaatgaa aagaaaggat ttagcatgca
     3361 aaaattcaaa gacttttttt acgatgattt atcggttaca cgaggaaatt attttttaac
     3421 tttaatggca gcatttttta ttactatcat tttatttatc ggcatagttg tcagtgaagt
     3481 atatttactt tatagcatgc tgattgtatt agtaggttta attctattga ggctattcaa
     3541 aatcaattta ttctctttta aaaaattaac attgtctcaa gttatttata ttataggcgg
     3601 tgcactatta atttatgggt tagataatct ttatttatat tttcatgacg taccggcaaa
     3661 tgaacaacaa ttagagcaag aaatacgaaa tacaccattc tatatttcta ttttcactgt
     3721 taccatcatc cccgctattg tggaagaaat tgtttttcgc ggtatgataa taagggttat
     3781 cttcagaaaa cacttgtttt tagggttaat tgtgtctagt ttagtttttg catcattaca
     3841 cgaatctgac acttggattg gttatttacc ttacttatat tcaggtttga tttttggtct
     3901 aatttatata aaaacaaaac gattagaagt ggtaatattc atgcacttct taaataactt
     3961 gttagctctg ctctttataa tatggagata attttgagat aacctttcat attattttca
     4021 aattatctat ctgatattta ttttattgaa cttgagtcac cacttaaact caaaaatacg
     4081 tgtcatcaag tgcaaaataa tttagaacta atacaaacat aaaaactttt gctttacgct
     4141 taaaaatcaa aagtcacata cctagatata agtacaactt gtatattttg tactctaata
     4201 ggtatgtggc ttaaataata tttaattctt gagttgcata tagtaattgt ttttattcat
     4261 tttctaaaat atgcgagact aatttccttt tctacttctg ttgaatcatt ttagactttt
     4321 ttataactcg agcttttact tagctgaatt ttgacttcta cttcattaat aactatgatg
     4381 gaaaaacccc cataagcaca atgcctatga gggtttaaaa atatttatat attatagaat
     4441 agaaagacct gaagattgaa tatctttcgc aaagccttta actgtatcta ctgataattc
     4501 gttaatatcg cgacctaagt ttgtattcac ttttttcaca acatctgcag ggcatgtaat
     4561 aatatctgca ccaatttcat cagcttgaat cacattgaat aattcgcggc aacttgccca
     4621 caataattta acgccgtctt tactatgcgt aacttttaca gcctctttca ttaatggtaa
     4681 tggatctacg cctgtatctg caatacgtcc tgcaaatact gaaacatatg ttggcacacc
     4741 ttcagttact gcttcagtta tttctttaac ttgttcaatt gtataaacag ccgtaacgtt
     4801 taatctcaca ttgtcagctg aaagtttttt aattaaagga atcgttgatt caccttttgt
     4861 atttacaata ggaattttaa caaatacatt ttcgccatat tgttttaaaa ttgctgcttc
     4921 tttttccata gtttctaaat cgtctgcaaa tacttcaaat gaaattgaag catctggaat
     4981 ttctttcaca gcttcttcag caaaagcttt gtaatctgtt acgcccgctt tcgccattaa
     5041 actaggattt gttgtaaaac catccacttg tttgttttta taagctgctt tcatttcttc
     5101 aatatctgca ccgtccgcaa atacttctac atttagttta gccatataat atagcctcct
     5161 tgattcttat taaaatttaa caacatctgc atgtcttttt cttacaccat ttgtaaaaaa
     5221 tgatttttat ttctttgtta tataaaacat ttatatcacg cttttataat atatagctaa
     5281 ttttttgcct atatttttta aaaatgactt tagcaacatt gtgtcaacaa ggtaattaaa
     5341 ctattgtttt atgattgttt atgatacgat atgtaaatga taaccatcca ggaggtctag
     5401 catgtctcgt tcaaaaaaat acttttactt atctagctta atgattattt taagcttttt
     5461 ctttaataca aataacgttt tcctaagtgg actttttaat tcttttatta aattaatact
     5521 tttctgcagt gttattaact caattgtact aattttgtct ataatttttg cagatcgttc
     5581 aattaaatca ctaaagcctg atgcagattg gattagaatt gcgagtaaaa gtttgccttg
     5641 gattattcta atcgttattt tagtacatat cttttcaatt gttcgtacat tcggttttat
     5701 ttaaaaaaga caaaatgtca atgtagcctt aatacaaaac aatacaatgt ttcatgccat
     5761 aataagtaaa tcaatttgat gacgttgttg cgtataaaaa atattagatt ttcgaaatca
     5821 taactatgca tctaatcgct atagttatac aacaagatat aacatataat gaggtttgat
     5881 aatgcatcga caatttttgt cgtcgcgttg ccaaaacctc ttttttaaat tcaaactact
     5941 tcttttcgag gtgaagcaaa tgcaatatgt atatattttt atcggtggtg ctttaggcgc
     6001 tttattacgt tacctcattt cttttctgaa tactgacgga ggttttccaa tcggaacact
     6061 ggtagccaat ttgactggtg cctttgtaat gggattgcta acagccttaa caattgcatt
     6121 tttttcaaac catccgaccc taaaaaaagc tattacgact ggttttcttg gtgctttaac
     6181 gactttttca acatttcaat tagaattaat acatatgttt gatcatcaac aatttataac
     6241 tttactacta tatgctataa caagttatgt ctttggtatt ttgttatgtt acgtcggtat
     6301 aaaactaggt ggtggtttat catgatatca atcattttag tcatgattgg cggcggtttc
     6361 ggtgcaattt ctagaagtgc cattactgat tattttaatc ataaatttac ttcaaagtta
     6421 cctatcgcaa cattgatagt aaatctagtt ggtagttttt taattggatt aactataggc
     6481 ttatcaattt caatctcatg gttccctgcg ttctttgtta ccggtttttt aggtggctta
     6541 acaactttct caacgttagc caaggaactt acactaatga tgacgccaaa atttaatatt
     6601 aacctttttc tcaattattc acttttacaa ttcatcattg gatttatagc ttgttatatt
     6661 ggctatcata tttaaaaata aaatgcttca ttcagcaaat aggtaaatta cgacaccttc
     6721 ctgaacgaag cattttttaa ttttcatgca aattttttag caccatataa tgcctatcaa
     6781 aataaaacaa tctttgttgc cgtttaaata atgtgaatgt caataaattc tccaaactag
     6841 tcgaaaataa aacgttaagc aatattgcta ttacctaacg ttgacaattc atctaacctt
     6901 ttcatcagtt gagcgatttt aatctagcct tcaaattttt ttggatcagg tccaattctc
     6961 ttatcttgat ttaaaccatc aattcgcgtc atttgttcat ctgataattc gaaatcaaat
     7021 atttggaagt tttcagagat tctgtttggt gtcaccgatt tagggattgt aaccacacca
     7081 tgctgcacat tccatcttaa aacaacttgg gcaggtgact ttcctaattc ttgagcaatg
     7141 tctttaattg tctcatcatt taaaatttgt gcattcatca atggtgacca agattccatc
     7201 acgatatgtt gtgctgccaa atataatttc aatttatgtt tcgttaaata tggatgatat
     7261 tcaacttgat taataacagg tttaattgac acttgtgcca acaaagcttc caaatgttca
     7321 ggttcaaaat tgctgacacc tatattttta actttattat ttttatataa atcttccata
     7381 cctttccatg tatcaaccat tactgcttcg ttcgtacctg gccaatgtac tagatacaaa
     7441 tctaagtatt ttaaacctaa tctagataaa ctagcttcgt aagcagctgc tacattttca
     7501 cgaccaaaat cctcaaaata taattttgaa gtaataaata agtcttctct agcaatacca
     7561 gttgattcca atccggcacg aatgccagca cctacttgtt cttcattccc ataaactttt
     7621 gcggtatcaa tactacgata tccttgttca atggcatact taacactttc catgcaattt
     7681 tcatcatttt ccacacgaaa tgtccctaaa ccaatttgtg gcatcgtgtt tccattataa
     7741 aatgttttaa cctccataaa tatcgcctca cctttttgat gtattatacc ctgttatcat
     7801 aacaaatctg agttgaatac atgagaaaaa acacttagag caatcaacca ctaaaattct
     7861 agtaatatct ctcaaatatt aatcaaattg taaaagtaat tctgtttaat ttatgacaaa
     7921 ctaaaaaagc cgaagtaaca acatatagtc atcacttcag cctaacattt aattgaatga
     7981 ttcaatttta tccatcattt gttgtaagtc ttccacgttg tattgaatac gaccatggaa
     8041 tacaaatttg ttaaagaact cgtctaattg ttcagcacca acaagcactt tgacagcact
     8101 attttgatta taatttgaaa tcgttacatc gccttcattt ttaggattaa agtataaaat
     8161 tgaagtcggt gtatatttag cacctaaatg tttttgtaag tcttcagcca attgtttaat
     8221 cgcctcaatt tgatctgaat aatttacaaa tgataatgaa cgtttgtcat cattttgatc
     8281 catcacaata gtttgcggtc tagatttatc taaatctaat gtatcaaata cttgttccat
     8341 tggtggtaaa tctttaaatt gaccgccact aataccatta taaacatgac cttttaacaa
     8401 ttgagaatca ataatataaa gaccagttct tgttaatact aaatgactaa ttcgttcaat
     8461 attattaaag ccatcctttg gtaaaaagat atttgccata atgtgcatat cttctggtcg
     8521 aattcgtttt tctttaacta atctttcacg aataccaatt aatctcatgt ccgttacata
     8581 ttcactatga tttttcgaga acaattttaa tgcgtcaatc tcacgatctt ttgtactaac
     8641 catgtgatta taatcttctt gttgttttgt aattgtcttt ttattttgaa tacgctcttt
     8701 ctctaaagct tcttcatgag actttttaat gttttgttct tgttgttcat acttttcttc
     8761 tgtttgtcgc ttaacttttt tcttactacc taaggcaact aagaaaagga caaaaaagat
     8821 taatgcaatt actactgcaa taatgagtcc aatgactacc ggtgaagata aatccatcac
     8881 aacaacgctc ctttttaata tatgaataac tttaattata atagaaaagc taaagatttt
     8941 cgatacataa tatcatttat ataccgaaaa tcttttattt agccttattt agttcgaatc
     9001 attactttac tgcgtctttt aattcttcca ctttgtctaa tttttcccat gggaataaga
     9061 catctgtacg tccaaaatga ccataagcag cagtttgttt gtaaatcggt tgtttcaaat
     9121 caagcatttt aataatacct gcaggtctta ggtcaaagtg ttttctaact gcttcgacaa
     9181 gttgtccttc agaaacttta cctgttccaa atgtatcaat tgcaattgac actggttctg
     9241 caacaccaat cgcatatgct aattgtactt cacattgatc tgctaaacct gctgcaacaa
     9301 tatttttagc cacataacgt gcagcgtatg cagctgaacg gtctactttt gtaggatctt
     9361 taccactgaa gcatccgcca ccatgacgtg catagccacc gtacgtatca acaatgattt
     9421 tacgtcctgt taatcctgca tcaccttgag gtccaccgat tacaaagcgt cctgtaggat
     9481 tgatgtagaa tttagtttgt tcattaatca agttttctgg aacagttgga taaatgacat
     9541 gagctttaat gtcttcttga atttgttcaa gtgtcacatc ctcagcatgt tgtgttgata
     9601 cgacaatcgt atcaatacgt acagggttat cattttcatc atattcaaca gtgacctgaa
     9661 ctttaccgtc tggtcgtaaa taatttaacg taccatcttt acgcacatca gataaacgtt
     9721 ttgccaattg atgtgataaa taaattgcta gaggcatgta cgtttctgtt tcattcgttg
     9781 cgtaaccaaa cattaaacct tggtcacctg cacctgttgc ttcaatttct tcttcgctat
     9841 ctttatcacg atactctaat gctttatcca cgccttgtgc aatgtcaggt gattgttcat
     9901 caatcgcagt taaaattgcc attgtttcat aatcataacc atattttgct cttgtgtatc
     9961 caatttcttt gattgtttct ctaacaactt tcggaatatc aacatatgtt gttgtagaaa
    10021 tttcgccggc gatcaatgcc atacctgttg taacagttgt ttcacaagct acacgtgcat
    10081 ttgggtcgtc ttttaaaata gcatctaata ttgcatctga cacttggtca gcgattttat
    10141 ctgggtgtcc ttctgtaaca gactctgaag taaataatcg tttgttattt aacatagttt
    10201 gctcctttaa atttatatta cgaaaattct ctctctgtga gctaaataaa aaagaccttc
    10261 taactattaa tatagagaga aggcctaata cgtccattcg ctcttatcgt tcagacctat
    10321 ttgtctgcaa acggtttggc acctttcttt tataaaaaag aggttgctgg gtttcattgg
    10381 gtccatgtcc ctccaccact caggataaga gaatccgtta aaaataatag tacctaatta
    10441 atgaattaat gtcaattttt cacaaataaa tttacagtaa aatattgtag attaattatg
    10501 ttaatgtgtt atactaatta aatgtaaagg cttacattta aattatcgct ttggagggat
    10561 ttaggatgtc agtagacaca tacactgaaa caactaaaat tgacaaatta ctgaaaaaac
    10621 caacgtcaca ttttcaactt tcgacgacac aactttataa taaaatctta gataataacg
    10681 aaggggtatt aacagaactt ggtgctgtta atgcaagtac tggaaaatat actggtcgtt
    10741 cgcctaaaga caaatttttt gtctctgaac cttcatatag agataacatt gattggggag
    10801 aaattaatca acctatcgat gaagaaactt tcttgaagtt ataccataaa gtactagact
    10861 atttagataa aaaagatgaa ttatacgtat ttaaaggcta cgctggtagc gataaagata
    10921 caatgttaaa acttacagtc atcaatgaat tagcatggca taatttattt gctaaaaata
    10981 tgtttattag acctgaatca aaagaagaag ctacaaagat taaacctaac ttcactatcg
    11041 tttctgcccc acattttaaa gcagatccag aagttgatgg tactaaatct gaaacttttg
    11101 tcattatttc atttaaacac aaagtcattt taatcggcgg tactgaatac gctggtgaga
    11161 tgaaaaaagg tatcttctct gtaatgaatt atctcttacc gatgcaagat attatgagca
    11221 tgcattgctc agcaaacgtt ggtgaaaaag gcgatgtcgc attattcttt ggtctatctg
    11281 gcacaggtaa aacaacctta tcggctgacc cacaccgtaa actaatcggt gatgatgaac
    11341 atggttggaa taaaaacggg gtctttaata tcgaaggtgg ctgctatgca aaagcaatta
    11401 atctttccaa agaaaaagaa ccacagattt ttgacgcaat caaatatggt gcaattttag
    11461 agaacactgt agttgcagaa gatggttcag tggactttga agacaatcgt tatacagaaa
    11521 acacgcgtgc cgcttatcca attaatcaca ttgacaatat tgtagtacca tctaaagcag
    11581 cacatccaaa tacaattatt ttcttaactg cggatgcatt tggtgttatt ccaccgattt
    11641 caaagttaaa taaagaccaa gcaatgtatc atttcttgag tggtttcact tctaaattag
    11701 ctggtacaga gcgtggtgtg acagaacctg aaccatcatt ctcaacatgt ttcggcgcac
    11761 cgttcttccc gttacaccct actgtttacg ctgatttatt aggtgaactt atcgatttac
    11821 atgatgttga tgtttatctt gttaatactg gatggactgg cggaaaatat ggtgtaggac
    11881 gtagaatcag cttacattac acacgtcaaa tggtaaacca agcgatttct ggcaaattga
    11941 aaaatgcaga atatagaaaa gatagtacgt ttggtttaag tattcctgta gaaattgaag
    12001 atgtaccgaa aacaatttta aatccaatta atgcttggag cgacaaagag aaatataaag
    12061 cacaagcaga agatttaatt caacgttttg aaaagaactt cgaaaaattt ggtgaaaaag
    12121 ttgaacatat tgctgaaaaa ggtagcttca acaaataaat ttgaatacta attaaagagc
    12181 caaccattgt tatagtctaa caatggttgg ctccccttat tttatatggt aagagtttat
    12241 aggcaaattt attacaacaa tgcacattta tggtgacctt catgccaaac tcgcatcact
    12301 cattcaatgg aagcagcacg tcttcgcata aagtaccaat tcctaattca atatatatca
    12361 cacatcttca aagattgaaa gttaacctgt gcttatcacg tatcatacta gctatgcaac
    12421 tcaacttggt tcataaactc tttaatataa gtcaatgttt caaccatcgc tggtggtctt
    12481 ggcacatgtc cttctgccat ttgataaaat gtttcatgcg tgacaccttt taactctagt
    12541 tgatccgcta aataatacgc atgatgaata ccaacttgct ggtctttccc tccatgtaca
    12601 attaatatcg gcggactgtt ttcattaatg tttggaatcg cttggcgtgc ctcatatgcc
    12661 gctcgatctt ttttcggatg accaatcatt cttcgtaaca tccctcttaa atcaacgcgc
    12721 tcttcataca tcaaatcaat atctgagact ccaccccaga tgatataact acttactggt
    12781 agatcttgaa acgtcaacaa cccttgtagt ccacctcttg aaaaaccaac catatgaata
    12841 aaagcttgcg gatatttatt atgtagcaac cttaatagtt gcgtcacatc atttaaatcg
    12901 ccacgataaa attcatcttt accttcactg ccattgttac ctcggtagta tggcccaatc
    12961 actaaagttt gactatctga aaattgcatt aatctacctg cgcgcacacg tcctacttgg
    13021 cctttgccac ctcgcaaata tactacaatt cgttttactt catgacgtgg tgtcatcatt
    13081 aaagctttta cttgtaagtc atctgacaaa tatgtaattt cttcgaattg atgcgtaaaa
    13141 gattcaattg gcattcgttt acgtttgata aaatccaagt gattgcaccc tctctacgca
    13201 ttttaaaatg gtactatctt gcagtaagaa actccgttgt gcgagttcaa tatcattgat
    13261 acagttaaac aacactggcc ctgctgtttc caaataatcg ttcttgctta ccaatgattt
    13321 aacttcgata aaatatacat cttttacaaa atcaatttga tcatgtgttt caatggtata
    13381 ttgtgctatg taatgaatat ttttaacttt ggcgcctgtt tcttcatata attcacgggt
    13441 aactgcttca gcactacttt ccccgcgttc ccttttacca ccaggaaatt caataccccg
    13501 tatattatgt ttggtaaaaa gcaattgatt tttaaacgtt ggaatagcta gcacatgatt
    13561 gccatctgct acctcattat cctttttaaa tgtcaaaata acttgacgat tatctttatc
    13621 cctaaacttc acgcgcatca catccctaca ttgtatgtta atataatagt taattactat
    13681 cgttggaggc attaattatg aaaaagatat tcttggcgat gattcatttt tatcaacgtt
    13741 tcatttcgcc actcactcca ccaacttgtc gtttttatcc aacatgttca gagtacacta
    13801 gagaagcgat tcaataccac ggtgctttca agggccttta tttaggcatc cgtcgtattt
    13861 taaaatgtca tccgcttcat aaaggtggct ttgaccctgt tccgttaaaa aaagacaagt
    13921 cagcaagcaa gcattcacat aaacataacc attaatatgg ttgtaattga gttaaatcca
    13981 ctaaaggggg gcgaaattcg agtcgcccct cttttaatat gcctgaatgc gccaccacat
    14041 cttgttcaaa ataataacct gctggtgtaa catctcctga ataatcacct ttacgagcta
    14101 gcatcgctgt aaaataacgg cttaaaccat attcgtacat gccgccaata accactttta
    14161 caccatgact tttcaaagta tcaattgccg tttgcacttt atcaatgcca cctagacgaa
    14221 atggttttaa tacaacaact ttcacattgt ataattctat caaattaatt atgtccgaca
    14281 gtgacgtcgc cttttcatca agggctattg gaggtattgt tccatccact acttcatcaa
    14341 gcattgagat atctttaaat ggctcttcga tataaagaat atgttcacgc gctaataact
    14401 gtaactgtgt gaaatcttga cgatccaagg actcatttgc atctataacc aattgaaagt
    14461 ggaagtctaa ttcccgtaac actctaattt gatgcatgat ttgaggtgtc cattttaatt
    14521 taattctggt cggctttgtt gctttcaatg actctagttg tttatttgat aagccgctcg
    14581 ctgtcgctcc atatgctact gaaaatgaag gcagtacatg aaacatttga tacaatgcca
    14641 tgacaatagt tgcccttgca gcaggcgtat tttccaaagg tgccaataat tcaagtgccg
    14701 cttcatacga ttcgaatgat ttatttttaa tacctataaa ccattgttga agtgcttctt
    14761 ttactgaatc aatcgtttca aaattatacc aatctgtttg aaaagcatta cattcaccga
    14821 aatatgaata gcctttatca tcaattatct caataaacaa acaatcacga tgagttaaag
    14881 tgactttcgg agttacaatt tgcgacttaa actgttcact atatttataa aaatgcaaag
    14941 ccgtcagttt cactagatca ccctctattc aacttatttc tttgtaattt accagtcgat
    15001 gtatatggca acgtatcaac cttttcaaag tatttcggta ctttatattt cgctaaatgt
    15061 tgtgataaat atgcaatcaa ttgtgccttt gaaatgttac tttcactgac aaaatataat
    15121 ttaggtacat gtccccaagt atcatcagga tgccctacac atactgcgtc actgatacct
    15181 ggaaattgct tcgctaccgt ttcaatttga tatggataaa tattttcacc gccactaata
    15241 attaaatctt tacgtcggtc ataaatcatg acataacctt catgatctat ttcagcaatg
    15301 tcacccgtat taaaataacc attttcaaac gtacccgtta aatctgttgg atacaaatat
    15361 ccattcataa cattggcgcc tttaatcatt aattctccat gaccttcttt attaggattt
    15421 ttaactttta cgtcaacatt ggcactaggc atccctacag tgtcaggacg tacatgcaac
    15481 atttccggtg ttgctgttaa aaattgcgaa catgtctcag tcataccaaa tgaattataa
    15541 attggcaggt tatattgtaa tgccgtctct atcatagtgg cagataattt agcaccgcca
    15601 agtagtattt tttgcaaatc ataaggttca tgtaaacctt gttgcataag ccaatttaaa
    15661 gtttgtggca ccagcgaaat gtgtgtgatg cgttcatttt ttatcatcgt taaaatttgt
    15721 tcggcattaa atttatcaac aatgcgcaca gtaaaccctt caataacagc tcttaaaagt
    15781 acactgagac ccgaaatatg ataaatcggc aagacagata gccaattagt atcacgatca
    15841 aatcccaagc tctctttaca tccgattgca ctggcataat gattacgaaa cgtttgtggc
    15901 accgcttttt gagggccagt tgtccctgat gtaaacataa tcgatgcaat gtcatctaaa
    15961 ttaaatgatg tatttaatat gttgggcagc gaatctttcg gcaccacagt ttcattcgat
    16021 gtatcatatt ggatatccat tgtgttgtcc aacaaaccgt tcattgtaat atccgttcca
    16081 gcgaattcaa tatcatccag cgatacaatt tgaaaccctc gcaattccag aggtaaggtg
    16141 caaaaaatca attgtacatc gattgacctc atctgattct tcatctcatt aggtgtcaac
    16201 cttgtattaa tcatcgcaat ttcaatattt gccaaccaac atgcatgaat taaaatgact
    16261 gattgaatcg aattatctat gtatagcccg acacgagatt gttgataagc cttgagtctt
    16321 ttagccaata gactcgcttc acagtataaa ttatgataag tataagattc ttgaccgtct
    16381 gttatcgcaa tatgatgtcc attttgttgt gcttgtttat ataaccaaaa gtccatgcgc
    16441 tattcctcca aaatcattta cattataatt ataacgattt tatgacattc tagcagtgat
    16501 tatgtttaca aatataaaaa agtagacgaa ttgatgcatt gatatgattg ttataatgct
    16561 caatacatat cgttatatca ttcgtctact aaatatcagt tatttttatt taattttagt
    16621 gtcattctat cattttgatg tggtgattta cccattgttg ccacatcatc tgcaatgtca
    16681 attggtatac gattaatgtc ttgtaatgca cttaaatgga atacttcatc atctaaattt
    16741 tcaatgacat atacataata tgttaccttg tcctttttat attttaacgt tttccaaaag
    16801 tccggcttgc aattcaatac attatccgga atatattcaa taaataagta acgtttgctg
    16861 cctactttgt ctacgaaata ttttacagtg cctttttcta tacctcttat atgtgcatag
    16921 tctgctgaaa agtaaatact acctattgtt tcattatgtt gttgtatttc aaatcgttgg
    16981 cctactattt tattatttgt gccacaacta cttaaaaaaa tcagtagata tagcattaaa
    17041 catattttca tccccttgaa ttttaaaaac tttttcaaaa gcaacacctc taaaaataaa
    17101 tacaatatat tatagcaaat aga
//
LOCUS       DAHHRR010000048        16313 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.048, whole genome shotgun sequence.
ACCESSION   DAHHRR010000048 DAHHRR010000000
VERSION     DAHHRR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16313
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.048"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            121..414
                     /gene="esxA"
                     /locus_tag="NBI42_001392"
     CDS             121..414
                     /gene="esxA"
                     /locus_tag="NBI42_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HCD1777815.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            497..3526
                     /gene="esaA"
                     /locus_tag="NBI42_001393"
     CDS             497..3526
                     /gene="esaA"
                     /locus_tag="NBI42_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HCD1777816.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQSSLFDEQNTAMDKVLQDYKS
                     QKNSVELDNYINVLKQMDSQIDQQSNMQDTGKEEYKQTVKENLDRLRDIIKSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLTEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLALVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            3526..3984
                     /gene="essA"
                     /locus_tag="NBI42_001394"
     CDS             3526..3984
                     /gene="essA"
                     /locus_tag="NBI42_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HCD1777817.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            3956..4198
                     /gene="esaB"
                     /locus_tag="NBI42_001395"
     CDS             3956..4198
                     /gene="esaB"
                     /locus_tag="NBI42_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HCD1777818.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            4211..5545
                     /gene="essB"
                     /locus_tag="NBI42_001396"
     CDS             4211..5545
                     /gene="essB"
                     /locus_tag="NBI42_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HCD1777819.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTSFLDEQYQKQEQDYSENYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAIDIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            5567..10006
                     /gene="essC"
                     /locus_tag="NBI42_001397"
     CDS             5567..10006
                     /gene="essC"
                     /locus_tag="NBI42_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HCD1777820.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYSSFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGVATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMILQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LSLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHHVANFAEEREDIRAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            10036..10428
                     /gene="esaC"
                     /locus_tag="NBI42_001398"
     CDS             10036..10428
                     /gene="esaC"
                     /locus_tag="NBI42_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HCD1777821.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            10444..10758
                     /gene="esxB"
                     /locus_tag="NBI42_001399"
     CDS             10444..10758
                     /gene="esxB"
                     /locus_tag="NBI42_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HCD1777822.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            10758..11432
                     /locus_tag="NBI42_001400"
     CDS             10758..11432
                     /locus_tag="NBI42_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HCD1777823.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLLIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            11432..11749
                     /locus_tag="NBI42_001401"
     CDS             11432..11749
                     /locus_tag="NBI42_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777824.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            11759..13603
                     /locus_tag="NBI42_001402"
     CDS             11759..13603
                     /locus_tag="NBI42_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HCD1777825.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEEIEEQKKKELAVDILAGV
                     GIIALTIVNPVAGAVAAGAYTAYSAANAATGKNIITGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSHISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNMKRAVTPQNHVTHGPKDSMVRSEGKHSVSSHEINSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRN
                     NYAQKTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKENGDWYKLEKE
                     WKEALTSGDEVKNIKMEVKYSGNSKRPIEFNVSYEINNKPKVKSIENI"
     gene            13614..14117
                     /locus_tag="NBI42_001403"
     CDS             13614..14117
                     /locus_tag="NBI42_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD1777826.1"
                     /translation="MTFEEKISKIYNEVANEISGMIPVEWEKVYTIAYVDDGGGEVVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVSFDYIDWMNSEFGPMGREDYYMYKKFGILPEKEYAINRVKKIEDYVKE
                     QEEAEDR"
     gene            14313..14996
                     /locus_tag="NBI42_001404"
     CDS             14313..14996
                     /locus_tag="NBI42_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCD1777827.1"
                     /translation="MTTKENIDTLRKPGAQALSLASLFMILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIIISLLQWIRFIDFEKESAQKYKKIYARFLVVINVLTTITAVFATC
                     NLYYFVALQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTRF
                     GLLLTALSAFIYIERIIEYILVPNVVESKFVIMVSIIIIACTQFVAFQFIMQYSRFYI
                     FELNKEDDD"
     gene            15105..15212
                     /locus_tag="NBI42_001405"
     CDS             15105..15212
                     /locus_tag="NBI42_001405"
                     /inference="COORDINATES: protein motif:HMM:NF028193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCD1777828.1"
                     /translation="MIIRIRKGTLAFVVISLIVFSCWLFILNMILSRLL"
     gene            15348..15566
                     /locus_tag="NBI42_001406"
     CDS             15348..15566
                     /locus_tag="NBI42_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777829.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            15656..16144
                     /locus_tag="NBI42_001407"
     CDS             15656..16144
                     /locus_tag="NBI42_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCD1777830.1"
                     /translation="MNFEEKLSEMYNEIANEISGMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSEDLNYYSDIPKDCNVSKDIFKNSWFKVYRMFKKLRNAFKEEGHEPWTSCEF
                     DFTRDGKLNVSFDYIDWIKLGFGPSGKENYYMYKKFGVLPETEYEINKVKEVEKYVKE
                     QE"
BASE COUNT         6345 a         2155 c         3065 g         4748 t
ORIGIN      
        1 ttaaaaaact atatacatat actaataatt tatatattat ttgagtaagg agcacttttt
       61 caaaaaatag tgtccctaaa aagttttgat aaacttaaaa tattcaggag gtttctagtt
      121 atggcaatga ttaagatgag tccagaggaa atcagagcaa aatcgcaatc ttacgggcaa
      181 ggttcagacc aaatccgtca aattttatct gatttaacac gtgcacaagg tgaaattgca
      241 gcgaactggg aaggtcaagc tttcagccgt ttcgaagagc aattccaaca acttagtcct
      301 aaagtagaaa aatttgcaca attattagaa gaaattaaac aacaattgaa tagcactgct
      361 gatgccgttc aagaacaaga ccaacaactt tctaataatt tcggtttgca ataagcattc
      421 tgaaattggc aaagtcacat tttctaatgt ggctttgctt atcatttttt aagaaaacaa
      481 ctgaaaggaa ataagcatga aaaagaaaaa ttggatttat gcattaattg tcactttaat
      541 tattataatt gccatagtta gtatgatatt ttttgttcaa acaaaatatg gagatcaatc
      601 agaaaaagga tcccaaagtg taagtaataa aaataataaa atacatatcg caattgttaa
      661 cgaggaccaa ccaacgacat ataacggtaa aaaggttgag ctgggtcaag catttattaa
      721 aaggttagca aatgagaaaa actataaatt tgaaacagta acaagaaacg ttgctgagtc
      781 tggtttgaaa aatggcggat accaagtcat gattgttatc ccagaaaact tttcaaaatt
      841 ggcaatgcaa ttagacgcta aaacaccatc gaaaatatca ctacagtata aaacagctgt
      901 aggacaaaaa gaagaagtag ctaaaaacac agaaaaagtt gtaagtaatg tacttaacga
      961 ctttaacaaa aacttagtcg aaatttattt aacaagtatt attgataatt tacataatgc
     1021 acaaaaaaat gttggcgcta ttatgacgcg tgaacatgga gtgaatagta aattctcgaa
     1081 ttacttatta aatccaatta acgacttccc agaattgttt acagatacgc ttgtaaattc
     1141 aatttctgcg aataaagaca ttacaaaatg gttccaaaca tacaataaat cgctattaag
     1201 tgcgaattca gatacgttca gagtgaacac agattataat gtttcgactt taattgaaaa
     1261 gcaaagttca ctattcgatg aacaaaatac agcgatggac aaggttttac aagattataa
     1321 gtcgcaaaaa aatagtgtag aacttgataa ctatattaat gtgttgaaac agatggatag
     1381 ccaaattgat caacaatcta atatgcaaga tacaggtaaa gaagaatata aacaaacagt
     1441 taaggaaaac ttagatagat taagagatat catcaagtca caagaatcac cattttcaaa
     1501 agggatgatt gaagactatc gcaagcaatt aactgaatca ttacaagatg agcttgcaaa
     1561 taacaaagac ttacaagatg cgctaaatag cattaaaatg aacaatgctc aattcgctga
     1621 aaacttagag aaacaacttc atgatgatat tgtcaaagaa cctgatacag atacaacatt
     1681 tatctataac atgtctaaac aagactttat agctgcaggt ttaaatgagg atgaagctaa
     1741 taaatacgaa gcaattgtca aagaagcaaa acgttataaa aacgaatata atttgaaaaa
     1801 gcctttaaca gaacacatta atttaacaga ttatgataac caagttgcgc aagacacaag
     1861 tagtttgatt aatgatggtg tcaaagtgca acgtactgaa acgattaaaa gtaatgatat
     1921 taatcaatta actgttgcaa cagatcctca ttttaatttt gaaggcgaca ttaaaattaa
     1981 tggtaaaaaa tatgacatta aggatcaaag tgttcaactc gatacatcta acaaggaata
     2041 taaagttgaa gtcaatggcg ttgctaaatt gaaaaaagat gctgagaaag atttcttaaa
     2101 agataaaaca atgcatttac aattgttatt tggacaagca aatcgtcaag atgaaccaaa
     2161 tgataagaaa gcaacgagtg ttgtggacgt aacattgaat cataaccttg atggtcgctt
     2221 atcgaaagat gcattaagcc agcaattgag tgcattatct aggtttgatg cgcattataa
     2281 aatgtacaca gatacaaaag gcagagaaga taaaccattc gacaacaaac gtttaattga
     2341 tatgatggtt gaccaagtta tcaatgacat ggaaagtttc aaagacgata aagtagctgt
     2401 gttacatcaa attgattcaa tggaagaaaa ctcagacaaa ctgattgatg acattttaaa
     2461 taacaaaaag aatacaacca aaaataaaga agatatttct aagctgattg atcagttaga
     2521 aaacgttaaa aagacatttg ctgaagagcc acaagaacca aaaattgata aaggcaaaaa
     2581 tgatgaattt aatacgatgt cttcaaattt agataaagaa attagtagaa tttctgaaaa
     2641 gagtacgcaa ttgctatcag atacacaaga atcaaaatca attgcagatt ctgttagtgg
     2701 ccaattaaat caagtcgaca ataatgttaa taagctacat gcgacaggtc gagcattagg
     2761 cgtaagagcg aatgacttga accgtcaaat ggctaaaaac gataaagata atgagttgtt
     2821 cgctaaagaa tttaaaaaag tattacaaaa ttctaaagat ggcgacagac aaaaccaagc
     2881 attaaaagca tttatgagta atccggttca aaagaaaaac ttagaaaatg ttttagctaa
     2941 taatggtaat acagacgtga tttcaccgac attattcgta ttattgatgt atttactatc
     3001 aatgattaca gcatatattt tctatagtta tgaacgtgcc aaaggacaaa tgaatttcat
     3061 taaagatgat tatagtagta aaaaccatct ttggaataat gtcattacgt caggtgttat
     3121 tggtacaact ggtttggtag aaggattaat tgtcggttta attgcaatga ataagttcca
     3181 tgtattagct ggctatagag cgaaattcat cttaatggtg attttaacta tgatggtctt
     3241 cgtacttatt aatacgtatt tactaagaca ggtaaaatct atcggtatgt tcttaatgat
     3301 tgctgcattg ggtctatact ttgtagctat gaataatttg aaagcagctg gacaaggtgt
     3361 gactaataaa atttcaccat tatcttatat cgataacatg ttcttcaatt atttaaatgc
     3421 agagcatcct ataggcttgg cgctagtaat attaacagta cttgtgatta ttggctttgt
     3481 actgaatatg tttataaaac actttaagaa agagagatta atctaatgtt gatgaatagc
     3541 gtgattgctt taactttttt aacagcatct agcaataatg gcggacttaa tattgacgtg
     3601 caacaagaag aggaaaagcg aatcaataat gatttaaatc aatatgatac aacgctattt
     3661 aataaagaca gcaaagcggt taatgatgcg attgctaagc agaaaaaaga acgacaacaa
     3721 caaataaaaa atgatatgtt tcaaaatcaa gcgagtcact cgactcgctt gaatgaaact
     3781 aaaaaagtgt tattttccaa atctaactta gaaaagactt cggagagtga taaaagcccc
     3841 tatattcaaa acaagcagga gaaaaaaata ttcccgtaca ttttgatgtc tgtaggggct
     3901 tttttgactt taggatttgt cattttttca attcataaag ggagacgaac gaaaaatgaa
     3961 tcagcacgta aaagtaacat ttgattttac taattataat tacggcacat atgacttagc
     4021 agtaccagca tatttaccga taaaaaactt aatagcttta gtattggata gtttggacat
     4081 ttcaatattt gatgtcaata cacaaattaa agtgatgacg aaaggtcaat tacttgttga
     4141 aaatgatcga ctcattgatt atcaaatcgc tgatggagat attttgaagt tactatagga
     4201 ggaaaaatag atggttaaaa atcataaccc taaaaatgaa atgcaagata tgttaacgcc
     4261 tttagatgct gaagaagcag ctaaaacaaa attacgctta gatatgagag agattcctaa
     4321 gtcttcaatt aaaccagaac attttcattt aatgtactta ttagaacaac attctccata
     4381 ttttatagat gctgaattaa ctgaactacg tgacagtttc caaatacatt atgacattaa
     4441 tgacaatcat acaccttttg ataatattaa atcatttact aaaaatgaaa aattacgtta
     4501 cttactcaat atcaaaaatt tagaagaagt aaatcgtaca cgctacacat ttgtgttggc
     4561 accagatgaa ttatttttca caagagatgg attaccaatt gctaaaacaa gagggttaca
     4621 aaatgttgtt gatccattac cggtgtcaga agctgaattt ttaacaagat ataaagcact
     4681 ggttatctgt gcattcaatg agaaacaatc atttgatgct ttagttgaag gaaacttaga
     4741 actacataaa ggaacgcctt ttgaaactaa agttattgaa gcggcaacgt tagacttatt
     4801 aacgtcgttt ttagatgaac aatatcagaa acaagaacaa gattatagtg aaaattatgc
     4861 atatgttcgt aaagtagggc ataccgtttt taaatgggtt gctatcggta tgacaacatt
     4921 aagtgtgtta ttaattgcat ttttagcttt tttatatttt tcagtaatga agcataatga
     4981 gcgcattgaa aaaggatacc aagcatttgt aaaggatgat tatacgcaag tactaaatac
     5041 gtatgatgat ttagatggta aaaaattaga taaagaggca ctttacattt atgccaaaag
     5101 ttatatccaa acaaacaaac aaggtttaga aaaagataag aaagaaaatt tacttaataa
     5161 cgtgacacca aattcaaata aagactactt attatattgg atggaattag gacaaggaca
     5221 tcttgatgaa gcgattgaca ttgccacata tttagatgat aacgatatta caaagttagc
     5281 gttgattaat aaattaaatg agattaaaaa taatggagat ttatcgaatg ataaacgttc
     5341 tgaagaaacg aaaaagtata acgataaatt gcaagatatt ttagacaaag aaaaacaagt
     5401 taaagatgaa aaagcgaaat ctgaagaaga gaaagcaaaa gcgaaagatg agaaattaaa
     5461 gcaacaagaa gagaacgaaa agaaacaaaa agaacaagca caaaaagata aagaaaaacg
     5521 tcaagaagct gaaagaaaaa aatagtatag gactgaggca aagacaatgc ataaattgat
     5581 tataaaatat aacaaacaat tgaagatgct caatttgcga gatggtaaga catatactat
     5641 tagcgaagac gagcgtgcag atattacgtt gaaatcgtta ggcgaagtca ttcatttaga
     5701 acaaaataat caaggtactt ggcaagcgaa tcatacttct attaataagg tgcttgttag
     5761 aaaaggtgac cttgatgaca ttacattaca gctttataca gaagctgatt attcatcatt
     5821 tgcgtatcct tcaattcaag atacgatgac aattggacca aatgcgtatg atgatatggt
     5881 tattcaaagc ttgatgaatg ccatcattat taaagatttt caatcaatac aagaatcaca
     5941 atacgtacgc attgtgcacg ataaaaatac agatgtgtat attaactatg aactacaaga
     6001 gcaattaacg aacaaagctt acattggtga tcatatctat gttgaaggga tatggctcga
     6061 agtacaagct gatggtttaa atgtattgag tcagaataca gtggcatcgt cattaattcg
     6121 cttaacacaa gagatgccac atgcacaggc agatgattac aatacgtacc atcgttcgcc
     6181 aaggattatt caccgtgaac cgaccgatga tattaagatt gaaagaccac cacagccaat
     6241 acagaagaac aatacagtga tatggcgttc cattataccg ccattagtaa tgattgcttt
     6301 aactgttgtc atctttttag tgagaccaat tggtatttat attttaatga tgattggtat
     6361 gagtacagta acgatagtat ttggtattac aacgtatttc tctgaaaaga aaaagtataa
     6421 caaagatgtt gaaaaacgag agaaagatta caaagcttat ttggataata aatctaaaga
     6481 aattaataaa gcgattaaag cacaacgttt tagtttgaat tatcattatc caacggttgc
     6541 tgaaattaaa gatatcgttg aaacgaaagc accaagaata tatgaaaaaa catcgcatca
     6601 tcacgatttc ttacattata agttaggtat tgcgaatgta gaaaagtcat tcaaattaga
     6661 ttaccaagaa gaagaattta atcaacgtcg tgatgaacta ttcgacgatg ctaaagaatt
     6721 gtatgaattt tacacagacg tagaacaagc accattaatc aatgatttaa atcatgggcc
     6781 aattgcatat attggtgcac gacatctcat tttagaagaa ttggagaaaa tgctaatcca
     6841 attgtcaacg ttccatagtt atcatgattt agagtttcta tttgtgacac gtgaagatga
     6901 agttgaaaca ttgaaatggg cacgttggtt gccacatatg acattgagag ggcaaaacat
     6961 tagagggttt gtttacaatc aacgaacacg tgaccaaatt ttaacgtcaa tttatagcat
     7021 gattaaagaa cgtatccaag ctgtacgtga acgcagcaga agtaatgagc aaattatttt
     7081 cacaccgcaa ttagtgtttg tcattacaga tatgtcatta attattgatc atgtcatttt
     7141 agaatatgta aaccaagatt tatcagaata tggtatttca ttaatctttg ttgaagatgt
     7201 gattgaaagt ttgccagagc atgtagatac cattattgat atcaagtctc gtactgaagg
     7261 cgaactgatt acgaaagaaa aagaattagt tcaattgaaa ttcacacctg aaaatattga
     7321 taacgttgat aaagaatata tcgcgcgacg tttggcgaat ttgatacacg tcgaacattt
     7381 gaaaaatgca attcctgata gtattacatt tttagagatg tataacgtga aagaagtaga
     7441 tcagcttgat gtggttaatc gatggagaca aaacgaaaca tacaaaacga tggcagtacc
     7501 tttgggtgta agaggtaaag atgacatttt atcattgaac ttacatgaaa aagcacacgg
     7561 accacatggt ttagttgctg gtaccactgg ttcagggaaa tctgagatta tccaatcata
     7621 cattttatct ttagctatta attttcaccc tcatgaagtt gcattcctat tgattgacta
     7681 taaaggtggg ggtatggcga acttatttaa agatttagtc catttagttg gtacgattac
     7741 aaacttagat ggcgatgaag cgatgcgtgc cttaacatca atcaaagccg aattgagaaa
     7801 acgtcaacgt ttattcggag agcatgatgt taaccatatt aatcaatacc ataagttatt
     7861 taaagaaggt gttgcgacag aaccaatgcc acatttattc attatttccg atgagtttgc
     7921 cgaattaaaa tcagaacaac ctgattttat gaaagaactt gtatcaacag cacgtattgg
     7981 acgttcgtta ggtattcatt taatacttgc gacacaaaaa ccatcgggtg ttgttgatga
     8041 ccaaatttgg tctaactcta aatttaaatt ggcattaaaa gtacaagata gacaagacag
     8101 taatgaaatc ttgaaaacac cagatgcagc agacattaca ttaccaggtc gcgcgtattt
     8161 acaagttggt aataatgaaa tttatgaatt gttccaatct gcatggagtg gagcaacata
     8221 tgacatcgaa ggcgataaat tagaagttga agataagacg atttacatga ttaatgacta
     8281 tggtcaactt caagcaatca acaaagactt gagtggactt gaagatgaag aaacgaaaga
     8341 aaatcaaact gagttagaag cggtcataga tcatatcgaa tctattacaa cacgattaga
     8401 aattgaagaa gttaagcgtc catggttacc accattacca gaaaatgtat atcaagaaga
     8461 tttagtagaa acagatttca gaaagttatg gtcagatgat gcaaaagaag tggaattaac
     8521 attaggactt aaagacgtac cagaagaaca atatcaagga ccgatgatat tgcaattgaa
     8581 aaaagctggt cacatcgcgt taatcggaag tccaggatat ggtagaacaa cgttcttaca
     8641 caacattatt ttcgatgttg caagacacca tcgtcctgat caagcacaca tgtacttgtt
     8701 cgatttcgga actaatggtt tgatgccagt cacagatata ccacatgttg ctgattattt
     8761 tacagtagat caagaagaca agattgcgaa ggcgatacgt atatttaatg atgaaatcga
     8821 tcgccgtaag aagattttaa gtcagtatcg tgttactagt atttctgaat atcgaaaatt
     8881 aactggtgaa acaattccgc atgtctttat tcttattgat aactttgacg cagtaaaaga
     8941 ttcacctttc caagaagttt ttgaaaatat gatgattaaa atgacgcgtg aagggctagc
     9001 attagacatg caagtaacct taactgcctc aagagctaac gctatgaaaa cgccaatgta
     9061 cattaatatg aaaacgcgta tcgccatgtt tttatatgat aaatcagagg tgtcgaacgt
     9121 agtaggacag caaaaatttg cggttaaaga tgtagtgggt cgagcattgt taagtagtga
     9181 tgacaatgta tcattccata ttggccaacc atttaaacat gatgagacta aatcatataa
     9241 tgatcaaatt aacgatgaag tatcggcgat gacagaattt tataaaggtg aaacaccaaa
     9301 tgatattcct atgatgccag atgaaattaa atatgaagat tacagagaat cattaagctt
     9361 gccagatata gttgccaatg gtgctttacc aattggatta gattatgaag gtgttacact
     9421 acaaaaaatt aaattaactg aaccagcaat gatttcatca gaaaatccga gagaaattgc
     9481 gcatattgcc gaaattatga tgaaagaaat tgacatatta aatgaaaaat atgcgatttg
     9541 tatcgcagac tcaagtggag agtttaaagc ttatagacat catgtggcta actttgccga
     9601 agaaagagaa gacattagag cgattcacca actaatgatt gaagacttaa aacaaagaga
     9661 aatggacggc ccatttgaaa aagattcact ttatattatc aatgatttta aaacatttat
     9721 tgattgcacg tatattccgg aagatgatgt taaaaagctt attacaaaag gaccagaact
     9781 tggcttgaac attttatttg tcggcattca taaagaatta atagatgctt atgataaaca
     9841 gattgatgtt gcacgtaaaa tgattaacca atttagtata ggtattcgta tttcagacca
     9901 acaattcttt aaatttagat ttattcaacg agaacctgtt attaaagaaa atgaagcata
     9961 tatggtcgca aaccaagctt atcaaaagat tagatggttt aaatagaaat gaattaaata
    10021 ggagggaggt atgttatgaa ttttaatgat attgaaacaa tggttaagtc gaaatttaaa
    10081 gatattaaaa agcatgctga agagattgcg catgaaattg aagttcgttc tggatattta
    10141 agaaaagctg aacaatataa gcgattagaa tttaatttga gttttgcact agatgatatt
    10201 gaaagcacag caaaggacgt acaaactgca aaatctagtg ctaataagga cagtgtaact
    10261 gttaagggaa aggcgcccaa tacgttatat attgaaaaaa gaaatttgat gaaacaaaag
    10321 cttgaaatgt tgggtgaaga tatcgataaa aataaagaat ccctccaaaa agctaaggaa
    10381 attgctggcg aaaaggcaag tgaatatttt aataaagcaa tgaattaata ttgaggtgaa
    10441 gatatgggtg gatataaagg tattaaagca gatggtggca aggttgatca agcgaaacaa
    10501 ttagcggcaa aaacagctaa agatattgaa gcatgtcaaa agcaaacgca acagctcgct
    10561 gagtatatcg aaggtagtga ttgggaagga cagttcgcca ataaggtgaa agatgtgtta
    10621 ctcattatgg caaagtttca agaagaatta gtacaaccga tggctgacca tcaaaaagca
    10681 attgataact taagtcaaaa tctagcgaaa tacgatacat tatcaattaa gcaagggctt
    10741 gatagggtga acccatgatg aaagatgtta agcgaataga ttatttttct tacgaagaat
    10801 taacaatttt aggtggtagt aaattgcctc tcgtaaattt tgaattgttt gatccatcaa
    10861 attttgaaga agctaaagct gctttaattg aaaaggaatt agtaacagag aatgacaagt
    10921 taactgatgc aggttttaaa gtggctacat tagtcagaga gtatattagc gccattgtaa
    10981 atattcgaat taatgatatg tattttgcac catttagcta tgaaaaagat gaatatattt
    11041 tgttaagccg gtttaaaaat aatgggtttc aaatacgaat tataaataaa gacattgcat
    11101 ggtggtcgat tgtacaatca tatcctttat tgatgagaca agaaaagtcc aatgattggg
    11161 actttaaaca aattgacgat gaaacattgg agaacttaaa taatgaaagt atcgatacga
    11221 ttgggcgtgt tttagaaatt gaaatataca atcatcaagg tgaccctcaa caaagtttat
    11281 ataacattta tgaacaaaat gatttgttac tcattcgata cccattaaaa gataaagtgc
    11341 tgaatgttca tattggtgtc attaatacat ttatacgaga attatttgga ttcgatactg
    11401 atgaaaatca tattaataaa gcagaggagt aatgacgttg agtggaaaaa ttagtgttaa
    11461 agctgaaacg attgcacatg ttgtaaaaga attagaaagc attagtcaaa agtatgatga
    11521 aatagctcaa aactttggaa aaatagcgca attaaattac tacagtagtg aaaaagctgc
    11581 acattctatg gaaaatggct atagtagtgc tgcaacagtc attagtggtc tcaaaggtcc
    11641 attgagtaca ctcggtggtg gcgtcatgaa ttcagcacaa aagttctttg aagcagatga
    11701 acattggggt acggaatttg ccaaacttta ctataatatt gagggatagg tgcatgacat
    11761 gacaaaagat attgaatatc taacagctga ttatgacaat gaaaagtcat ctatccaaag
    11821 tgtaatagat gcaatagagg gtcaagactt cttagatgta gatacaacaa tggatgatgc
    11881 ggtaagcgat gtcagttctt tagacgaaga tggcgcaata tcattaacga gtagtgtagt
    11941 aggtccacaa ggatctaaat taatggggta ttatcaaaat gagttatatg attatgcatc
    12001 tcaattagat tcgaaaatga aagaaattat tgacacgcca tttatagaag atatagataa
    12061 agcattcaaa ggtataacga atgttaaatt ggaaaatata ttaattaaaa atggcggtgg
    12121 tcatggtaga gatacctatg gggcttctgg gaaaattgca aagggagatg ccaagaaaag
    12181 tgacagcgat gtttatagca tcgatgaaat attaaaatcg gatcaagaat ttgtaaaagt
    12241 aattgatcag cattacaaag aaatgaaaaa agaagataag aaactatcta agagtgattt
    12301 tgaaaaaatg atgactcaag gtgcttcttg tgattacatg acagtagctg aagcggaaga
    12361 gatagaggag caaaagaaaa aagaattggc agttgatatt ttagcgggtg tcggaattat
    12421 agctttaact atagtcaacc ctgtcgcagg tgcagtagca gcaggtgcat ataccgctta
    12481 ttcagcagcc aatgcagcca caggaaaaaa tattataact ggaagaaagc tatctaaaga
    12541 agaacgcatc atggaaggac tttcgcttat tccgttgcca ggtatgggct tcctcaaagg
    12601 tgctgggaaa agtttaatga aattaggctt caaaggcgga gaaaaatttg cagttaaaac
    12661 aggattgcaa aagacaatgc aacaagcggt tagtcatatt tcacctaaaa tgggaatgat
    12721 gaaaaacagt gtgttgaatc aatctcgtaa ctttgctcaa aatactcatg ttggacaaat
    12781 gttgagtaac atgcgtggtc aagcaactca tactgttcaa caaagtagaa attggattgg
    12841 acaacaagca caaaatgtca aacgaatagt gaataatgga cttgataaag aaatagcaca
    12901 tccatttaaa caacaacttg caccagcggg aatgggtggt ataaaatttg ctgaaacaac
    12961 tactttgaga aacatgggtc aaaacatgaa acgtgctgtt acaccacaaa atcacgtgac
    13021 acatggtccg aaagatagta tggtgagaag tgaaggtaaa catagtgtaa gtagccatga
    13081 aataaattca tcaaaatatg ttgaatcacc aaactacacc aaggttgaat tcggagaaca
    13141 ctatgcgaga cttagaccta agaaactaaa agcaaatata gaatatacaa caccaactgg
    13201 tcacatatat cggaccgatc ataaagggcg cataaaagaa gtttatgtag acaatctctc
    13261 tctaaaagat ggagatcgta ataactatgc acaaaaaact gtaggtggag aggatagatt
    13321 accagacgat gatggaggtc atttaattgc tagaatgttt ggtgggtcaa aagacataga
    13381 taaccttgta gcacaaagta aatttatcaa ccgtccattt aaggaaaatg gtgattggta
    13441 caagttggaa aaagagtgga aagaggcact aacgtcggga gatgaagtga aaaatattaa
    13501 aatggaagtg aaatacagcg gtaatagtaa aaggcccata gaatttaatg tgagttacga
    13561 aataaataat aaaccaaaag tgaaatcaat agaaaatata taggagtgtt aatatgactt
    13621 tcgaagagaa aataagcaaa atatataatg aggttgcgaa tgaaattagt ggaatgatac
    13681 cagtagagtg ggaaaaagta tatacaattg cttatgtaga tgatggaggc ggagaggtcg
    13741 tttttaatta tactaaacca ggtagcgatg aattgaatta ttacacatat atccctagag
    13801 agtataatgt ctctgagaaa gtattttatg atttgtggac ggatttatat agattgttta
    13861 agaagttaag aaatgcgttt aaagaagaag atttagaacc atggacatca tgtgaatttg
    13921 actttacaag agacggcaaa ttgaatgtat cttttgatta tattgattgg atgaattcag
    13981 aatttgggcc aatggggaga gaagattatt atatgtataa aaaatttgga attttacccg
    14041 aaaaagaata tgcaataaat agggttaaaa aaatagaaga ttatgttaaa gaacaggaag
    14101 aagcggaaga tagataatct taatgtaaga ctaaacaata aagctttgtt tagtcttttt
    14161 agcgtttaag taaatagcaa tagagaatat aaagttgatt ctcatcaaca aataatataa
    14221 agataatttt atgctctgtg tagatgaaaa tcttagtata tagctttaaa tacgtaatat
    14281 gctatttaaa aaataatgta taggagatag atatgactac taaagaaaat attgatactc
    14341 ttcgaaagcc aggtgcacaa gccttaagtt tagcatcatt atttatgata cttttttcat
    14401 gtctaacttt cttttttggt ttagattatg aaaggtttcc taattattta aagataacga
    14461 caattataga attaataatt atcataatta gtttacttca atggattaga tttatagatt
    14521 tcgaaaagga aagcgcacag aaatataaaa aaatatatgc ccgattttta gtcgttataa
    14581 atgtgctaac tactatcact gcagtatttg caacatgtaa tctttactat tttgttgctt
    14641 tacaaaatca ttatgaccta ttcaattatt ggttgatggg cacgatttca atcataatta
    14701 gctatttgtt attagtaatt ggcggaatgt tcacgttatt aaaattacct aaagtaacta
    14761 aacgctgggg tggtaaaact aaaacccgtt ttggtttatt attaactgcg ttgagcgcat
    14821 ttatatatat tgaaagaatt atcgaatata tattggtgcc caatgtcgta gaatccaagt
    14881 ttgtcataat ggtaagtatc attattattg cttgtacaca atttgtagcc tttcaattta
    14941 ttatgcaata cagtagattc tatatttttg aattaaacaa agaagatgat gactaaataa
    15001 ttttataaat tactgttgga gtatacttat ataaaaaatc aacttcaaca cattattttg
    15061 ctcaatgatg tgaaagaatt tgagtggatt gatattaatg ggatttgatt attagaatta
    15121 gaaaaggaac gcttgctttt gttgtaataa gtctaatagt tttcagttgt tggttattta
    15181 ttctgaatat gattttatca agattattat aattgtatta catataggtg ttggtaaatt
    15241 tttatttacg aaatggtaaa aaagtatata ctattaatat cttttttact ataattataa
    15301 atagttaaat aaattgaaag acaaaataat gagtaggagt taatgagatg gaaaaccaaa
    15361 aacaaggcaa tggcttaaaa attgcaacat gggtatttat tgtattaaca gtagttacac
    15421 cgctatttgg tattggaagt attgtttgta gtattaatta caaaaaatac gatgctgaaa
    15481 aaggttcgaa gttattgcaa attgcaatta tcgtaacaat aattgctttt gttttaaatt
    15541 tattagcata tttaggttta agataagtaa aacgaatttg aagaagcatt aagcgacatt
    15601 tgggtgttgt ttaatgcttt ttatttaacg gaaataaaaa tataggggcg ataacatgaa
    15661 tttcgaagag aaattaagtg aaatgtacaa tgaaattgca aatgaaatca gtggaatgat
    15721 accggtagag tgggaaaaag tatatacaat ggcttatata gatgatggag gaggtgaagt
    15781 attctttaat tatactaaac ctggaagtga agatttgaat tattattcag atattcctaa
    15841 agattgcaat gtctcaaaag atatttttaa gaattcatgg tttaaagttt atcgaatgtt
    15901 taagaagtta agaaatgcat ttaaagaaga aggacatgaa ccatggacat catgcgaatt
    15961 tgattttaca agagatggta aattgaatgt atcttttgat tatattgatt ggattaaatt
    16021 aggttttggc ccatcaggaa aggaaaacta ctatatgtat aaaaagtttg gtgttttacc
    16081 agaaacggaa tatgaaatta ataaagttaa agaagtagag aagtatgtta aagaacaaga
    16141 gtagcagaca tgttatagaa ggatgtgcaa aatcactctc gttttacatt tgattcaaag
    16201 aagaaggtaa aaaataagat tatttgcaac ttaaaaagtc aattagctta tcgttatcca
    16261 tatatcatgg ataaatgagt ttaactaatt aataaatcgc gttataaatt taa
//
LOCUS       DAHHRR010000049        16256 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.049, whole genome shotgun sequence.
ACCESSION   DAHHRR010000049 DAHHRR010000000
VERSION     DAHHRR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.049"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            101..1723
                     /locus_tag="NBI42_001408"
     CDS             101..1723
                     /locus_tag="NBI42_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCD1777831.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            complement(1893..2456)
                     /locus_tag="NBI42_001409"
     CDS             complement(1893..2456)
                     /locus_tag="NBI42_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HCD1777832.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKGAQAIINPLLESSDLTEAETQEAQKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(2545..2640)
                     /locus_tag="NBI42_001410"
     CDS             complement(2545..2640)
                     /locus_tag="NBI42_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777833.1"
                     /translation="MKNVSIYKTTQELSFVSLLDFYLTKHLTLML"
     gene            2709..4199
                     /gene="betB"
                     /locus_tag="NBI42_001411"
     CDS             2709..4199
                     /gene="betB"
                     /locus_tag="NBI42_001411"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCD1777834.1"
                     /translation="MELLKHLSQRQYIDGEWVESTNKNTRDIINPYNQEVIFTVSEGT
                     KDDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            4460..6169
                     /gene="betA"
                     /locus_tag="NBI42_001412"
     CDS             4460..6169
                     /gene="betA"
                     /locus_tag="NBI42_001412"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCD1777835.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEVRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            6447..6659
                     /locus_tag="NBI42_001413"
     CDS             6447..6659
                     /locus_tag="NBI42_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCD1777836.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            6939..7382
                     /locus_tag="NBI42_001414"
     CDS             6939..7382
                     /locus_tag="NBI42_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCD1777837.1"
                     /translation="MNIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLQRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            7576..9174
                     /locus_tag="NBI42_001415"
     CDS             7576..9174
                     /locus_tag="NBI42_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCD1777838.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(9234..9563)
                     /locus_tag="NBI42_001416"
     CDS             complement(9234..9563)
                     /locus_tag="NBI42_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777839.1"
                     /translation="MTYSILNLYSILIQYKCKINHKKEIQYMHPRKTSQLPLILKIIL
                     NLALIGIVIIVLTHTEFQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
     gene            9859..11355
                     /gene="mqo"
                     /locus_tag="NBI42_001417"
     CDS             9859..11355
                     /gene="mqo"
                     /locus_tag="NBI42_001417"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCD1777840.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            complement(11548..12438)
                     /locus_tag="NBI42_001418"
     CDS             complement(11548..12438)
                     /locus_tag="NBI42_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HCD1777841.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVEGKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDSLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKADIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     ANLYKDLAEHPNVVRIVVLSGGYSREKANELLKDNAELIASFSRALASDLRAGQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(12561..12977)
                     /locus_tag="NBI42_001419"
     CDS             complement(12561..12977)
                     /locus_tag="NBI42_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777842.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDIISNQSHLKALLLNLDF
                     LIPSDNTPYTLEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLVFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            13231..14568
                     /locus_tag="NBI42_001420"
     CDS             13231..14568
                     /locus_tag="NBI42_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCD1777843.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMNQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALETGNLDQYDISGQGW"
     gene            complement(14648..16096)
                     /locus_tag="NBI42_001421"
     CDS             complement(14648..16096)
                     /locus_tag="NBI42_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1777844.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSIAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAVTFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
BASE COUNT         5599 a         2579 c         3056 g         5022 t
ORIGIN      
        1 taaatattag ttattgtcaa ttttgtgaat caatataatt attacatttt gagataaatc
       61 gaaacaggat tcataaaatt aataattagg gggagcacaa ttgaaaaaag agaaagttat
      121 ggactggacg acctttatag ggacagtagc tgtacttctt tttgcagtta tacctatgat
      181 ggcttttcca aaagcaagtg aagatatcat cactggtatt aatagtgcca tttctgattc
      241 aattggttcg atatatttat ttatggggct ggcgattttt tgctttgtaa tgtatatcgc
      301 atttggtaaa tatggtaatg tcacacttgg taaagcaagt gataaaccag aatttaatac
      361 atttacatgg gcggcaatgc tgttttgtgc aggcataggc tctgatattt tatactgggg
      421 cgttattgaa tgggcttttt actatcaagt accaccaaat ggcgcgaaaa gtatgagtga
      481 tgaagcactc caatatgcga cgcaatatgg tatgttccac tgggggccaa ttgcttgggc
      541 tatttacgtt ttaccagcat taccaattgg ttatttagta tttgttaaaa aacaaccggt
      601 gtataaaatt agtcaagctt gtcgtccgat tttaaaaggt caaacagata aatttgtagg
      661 taaagttgta gacatcttat ttatctttgg attgctaggt ggtgcggcaa catcactagc
      721 gttaggtgtg ccattaattt ctgcaggcat agaaagatta actggtttag atggtaaaaa
      781 tatgatttta cgttcggcca ttttattaac aatcacggtt atatttgcca ttagttcata
      841 tacaggattg aaaaaaggta ttcaaaagtt aagtgatatc aacgtttggc tatcatttgt
      901 acttttagcc tttatattta ttattggacc gactgttttt attatggaaa cgacagtgac
      961 agggttcgga aatatgttga gagatttctt ccatatggca acatggttag aaccattcgg
     1021 tggtattaaa ggtcgaaaag aaacgaattt cccacaagac tggacaatat tctactggtc
     1081 atggtggtta gtatatgcgc catttatcgg tttatttatc gctagaattt caaaaggtcg
     1141 acgccttaaa gaagtcgtgc taggaacaat tatttatgga acgcttggat gcgtattatt
     1201 ctttggtatt tttggtaact atgctgtgta tttacaaatt tctggacagt ttaatgtaac
     1261 acaatattta aatacacatg gtacagaggc aaccattatt gaagtggtgc atcatttacc
     1321 attcccatca ttgatgattg tactattctt agtatctgct ttcttattct tagcaacaac
     1381 atttgattcg ggttcatata ttttagcggc agcatctcag aaaaaagtag taggcgaacc
     1441 attacgtgcc aatcgtttat tctgggcatt tgcattgtgc ttattgccat tttcattgat
     1501 gctagttggt ggcgaacgtg cattagaagt attgaaaact gcttcaatac tggcaagtgt
     1561 gccattaatt gttattttta ttttcatgat gatatcattt ttaatcattt taggacgcga
     1621 tagaattaaa cttgaaacgc gtgctgaaaa attaaaagaa gttgaacgtc gttcattgcg
     1681 aatcgttcaa gtatcagaag aagaacaaga tgataattta taatttaata aatgatgcat
     1741 aagattttga attttataac tccataattg attgtcatat taagcaaaaa cggtaatttc
     1801 tatttattga gatagaaatt accgtttatt tcgtggacct attgcattgt tttcatcatg
     1861 cataatcatc attgtcgttg tttgagtcaa ttttaatttt cagaatcaga aggctgttct
     1921 ggaattggga aatatttgaa aatttcaccg ctttcaatcg cttcggttaa ctgttctaac
     1981 cattcgtaat aaacatgtgt atgatcaagc tgagctttaa ttttttgtgc ctcttgtgtt
     2041 tcagcttcag ttaaatcact gctttcaagt aatggattga taatagcttg agcacctttt
     2101 actgcttcga cattgatgtc aatttcacgc tgaaattttt tagtgaaaaa gtttcggaaa
     2161 aagatgaaaa agtctttctc ggcgataaaa tgttgtttgc ggcttcctct cgtaaattgt
     2221 tgtttaacaa tatcaaattc ctgcaatttc ttaacgccag cactcatact tggtttgctc
     2281 atttgcaatt gatgacgcat ttcatcaagc gtcatactgc cttcaaacac catagtgcca
     2341 tataagtttc ctacacttct attagtgcca tacaaatcca ttgtctgtcc aattgaatta
     2401 attacaatat cttttgcttg ttctaattgt tgctgtttgt tctgagaacg agtcatcatt
     2461 gcacctccgt acatcatttt ggtcacgtta aaataaatac taatacatta taaaaccttt
     2521 tctaaaaaaa gacattaaaa atatttaaag cattaaagtt aaatgtttcg ttaaataaaa
     2581 atctaacaaa cttacaaaac ttaattcttg agttgttttg taaattgaca catttttcat
     2641 ttctatgcta acataagttt gtaaaattcg ttaaataaaa atttaacaaa cttaacggag
     2701 gttgttgaat ggaactttta aaacatttat ctcagcgtca atatattgat ggtgagtggg
     2761 ttgaaagcac gaataaaaat acaagagata ttatcaatcc ttacaatcaa gaagtgatat
     2821 ttacggtttc tgaagggaca aaagatgatg cagaacgtgc aatcttagct gcaagacgtg
     2881 cgtttgagtc tggtgaatgg tcgcaagaaa ctgctgaaac aagaggtaaa aaagtacgtg
     2941 ccatcgcgga caagatcaaa gaacatcgcg aagcgttagc acgattagaa acattagata
     3001 ctggaaaaac gttagaagaa tcatatgcag atatggatga tattcataat gtgtttatgt
     3061 attttgctgg attagcagat aaagatggtg gcgaaatgat tgattcacca attccagata
     3121 cagaaagcaa aattgttaaa gaaccagtag gtgtagttac acaaattaca ccttggaatt
     3181 atccgttatt acaagcatca tggaaaattg cgccagcgct tgctacgggt tgttcactag
     3241 ttatgaaacc aagtgaaatt acaccattaa caacaatacg tgtttttgaa ttaatggaag
     3301 aagttggttt ccctaaagga acaattaatc ttattctagg tgcaggttct gaagttggtg
     3361 acgtaatgtc aggtcataaa gaggttgacc ttgtatcatt tacaggtggc attgagactg
     3421 gtaagcatat tatgaaaaat gctgctaata atgttacgaa tattgccttg gaacttggcg
     3481 gtaaaaatcc aaacattatc tttgatgatg ctgattttga attggcggta gaccaagcgt
     3541 taaatggtgg atatttccat gcaggtcaag tttgttcagc aggatcaaga atattagtac
     3601 aaaacagtat taaagacaaa tttgagcaag cacttattga tcgcgtgaaa aaaatcaaat
     3661 taggtaatgg ttttgatgct gatactgaaa tgggaccagt tatttcaaca gaacatcgta
     3721 ataaaatcga atcttatatg gatgtagcta aagcagaagg cgcaacaatt gctgttggtg
     3781 gtaaacgtcc agatagagac gatttaaaag atggtctatt cttcgagcca acagtcatta
     3841 caaattgtga tacgtcaatg cgtattgtac aagaagaggt tttcggacct gtcgttactg
     3901 tagaaggctt tgaaactgaa caagaagcga ttcaattagc gaatgattct atatatggtt
     3961 tagcaggtgc tgtattttct aaagatattg gaaaagcaca acgcgttgct aacaagttga
     4021 aacttggaac ggtgtggatt aatgatttcc acccatattt tgcacaagcg ccatggggtg
     4081 gatacaaaca atcaggtatc ggtagagaat taggcaaaga aggcttagaa gagtaccttg
     4141 tttcaaaaca cattttaaca aatacaaatc cacaattagt gaattggttt agtaaataaa
     4201 aattagataa ggtgagtgcc attgtaagga cacaagacac tcactttgtt ttgtataagt
     4261 ggcgaaatgt tgattgataa tttggactaa acgcaaaatg aatcatagat tatttcatga
     4321 ctgttagtaa caatcataaa agtaaaatcg agtgttttgg ttagctaagt ttagcaattc
     4381 aacgataacc aatcagccac taacaaatat ttcatgcaat actcactttg aaatacaaca
     4441 aactttggag gtcataacga tgagtaacaa aaataaatca tatgattatg tcatcattgg
     4501 aggaggcagt gcaggttctg tactaggtaa tcgtctgagt gaagataaag ataaagaagt
     4561 cttagtatta gaagcgggtc gcagtgatta tttttgggat ttatttatcc aaatgcctgc
     4621 tgcgttaatg ttcccttcag gcaataaatt ttacgattgg atttattcaa cagatgaaga
     4681 accacatatg ggcggtcgta aagtagcaca tgcccgaggt aaagttttag gtggatcaag
     4741 ttcgattaat ggcatgattt atcaacgtgg taatccaatg gactatgaag gctgggcaga
     4801 accagaaggt atggaaactt gggattttgc gcactgttta ccgtatttta aaaaattaga
     4861 aaaaacatac ggtgcagcgc cttatgataa atttagaggc catgatggac caattaagtt
     4921 aaaacgaggg ccagcaacga atcctttatt ccagtcattc tttgatgcag gtgttgaagc
     4981 aggctatcat aaaacacctg atgtgaatgg atttagacag gaaggttttg gaccgttcga
     5041 tagtcaagta catcgtggtc gccgaatgtc agcttcaaga gcatatttac atccagctat
     5101 gaagcgtaaa aacttaaccg ttgaaacacg tgcctttgta actgaaattc attatgaagt
     5161 tagaagagca actggtgtta cgtataagaa aaatggcaaa ctacatacca tcgatgctaa
     5221 tgaagtcatt ttgtctggtg gggcattcaa tacgccacaa ttactacaat tatctggtat
     5281 cggtgattca gagttcctaa aatcaaaagg cattgagcca cgtgttcatt tacctggtgt
     5341 gggtgaaaac tttgaagatc acttagaggt atacattcaa cataaatgta aggaacctgt
     5401 atcattacag ccaagcttag atatcaagcg aatgccattt attggtttac aatggatttt
     5461 cacacgtaca ggtgcagcag catctaacca ttttgaaggt ggcggatttg tacgttcaaa
     5521 taacgaagtt gattatccta acttaatgtt ccatttctta ccaatagctg taagatatga
     5581 tggtcaaaag gcagcggtcg cacatggtta tcaagttcac gtaggaccaa tgtattcaaa
     5641 ctctcgtggt agcttgaaaa tcaaatctaa agatccattc gaaaaaccaa gtatccgctt
     5701 taattattta tcaacagaag aagataaaaa agaatgggta gaagcaattc gtgtagcaag
     5761 aaatatttta tctcaaaaag caatggatcc attcaatggt ggagaaattt caccaggacc
     5821 agaagtgcaa acagacgaag aaattcttga ttgggttcgt agagatggtg aaacagcatt
     5881 acacccatct tgtagtgcta aaatgggtcc tgcttcagat ccaatggcag tagttgaccc
     5941 gttaacaatg aaagtccacg gtatggaaaa tttacgtgta gttgatgcat cagcaatgcc
     6001 acgtacgaca aatggcaata tacatgcacc tgttttaatg ttagctgaaa aagcagcaga
     6061 cattatacgt ggcagaaagc ctttagagcc gcaatatatt gattattata agcatggtgt
     6121 tcatgatgaa aatgaaggtg caatcgaagt gaaaccatac gctaaataaa ttcatatagt
     6181 agactcttag tctggcatct attgtattgg tgccaggctt ttttgcgttt catctaaatg
     6241 agaagttaat gtgttgttca aaaacggtgt gtgagtatat gaactgaaaa tagagttaat
     6301 tgtacatagt gataattaat tgacctaatt ggcagacatt aaaagagtgt acaaccttaa
     6361 aaagttcact aaatagacat ttaattgtgt tttcttgaaa gctttcgtgg taggatttat
     6421 ataagtaaat tgagagaagg aatactatga gcttttacga atatatacaa acatttaaag
     6481 atgataaaac accattaggc gaattagcga tttggattaa agaagatgat tcattcccaa
     6541 aacaagagaa gctaacagaa aacattttgt cttattttca tcaaatgtcc aatatagatc
     6601 atgagttttt agaaattgta aaaagatcac tttctctgta tgatcaatta aaatcgtaaa
     6661 gttagaaata aagttagcta aaataaaagg ccggtgattt cacagatgaa gttgtcggct
     6721 attttcttaa ctaatgtaaa ataatgatca tgaaattgaa gacacaaaaa tattgggcga
     6781 ctcctgtccc catttgtatg aaatattaat actttttaca aagtcttatc ttttaaaaag
     6841 aagccaaact atgattgtaa ttaataaata cgggaatgaa ttagtaatag atataaagta
     6901 atatgaccaa aaatataaat gaaatgaagg catttattat gaacatttat gatgaatata
     6961 gaagttatgt aatagaagaa ctggatgatt gtctcacaat acaaaaaaat aatgacactg
     7021 catattacga tgttctagaa gcgataaatg atttatcaaa tgattcatta tgtgtgttaa
     7081 atcatttata tattaacgaa ggacaagaag aaacatttga gcaaaaattt ttgcaacgta
     7141 ataagcattt aaaaaacgtt gatgggttca aagcattaag gtttttaaga cctagaacag
     7201 ctggtagaca ctatatcatt attacgctgt gggaaaatag gcaagcattt tatcattggc
     7261 aaaattcagc tgagtacaag catacacaca agcatcgtgg cacttcgaaa ggtgcagatg
     7321 taaaaataat taatcgtgaa ttatcataca atattcgtat tgaattagct gatatggtct
     7381 agcccgagat accaaccctc aatcatttga aagtccttga atgctacgtc atgtcaaagt
     7441 gtagtgtcga ggactttggt atttaaccct atttaattga aaattctgta aagataggcg
     7501 tatgatatat ataattcatt tataaaatta caatgttcat cacaggaaaa gtggatgtat
     7561 gaaaggaagt tagttatgca aaagtcagct ctattcgcgc ctgaaaaata taacattatt
     7621 tcggaaatcg aaaaatatag ccacatacca gataaaaagg caattctata tcataatact
     7681 gaaggcgaag atatatcagt cacttaccaa cagctcattg aacagtctaa taaagtaggt
     7741 aatgttttag catcgcacgg tttatctaaa ggcgataaag tactcattat gatgccacga
     7801 tctattgcga catatgaatt atatattgct gctttaaaat taggtgttgc aatcattccg
     7861 tgttctgaaa tgctaagaac taaagattta caataccgta tcacacacgg tgaaataaat
     7921 gcagtcattg cattagagcc attcacagtt gaatttgaaa aaattaaaga atatgatgcg
     7981 ttaactaaat ttgttattgc cggtcaaaaa gatggatgga tttcacttga agctgaaaaa
     8041 gaaaacgcaa gtaacaaact agaaatggca gatacgacgc gtgatgatat ggcgattctg
     8101 tcatatacat ctggtacaac tggcaatcct aaagccgtta cacattgtca tggatgggga
     8161 tttgcacatt tacaaatggc accaaaacat tggttatgta ttaatgaaga tgacttagtt
     8221 tgggcaacag cagcaccagg ttggcaaaaa tgggtatgga gtcctttctt atctgtatta
     8281 ggttcaggtg caactgcttt tgtatttaat ggaagattca gccctgaaac atacttagaa
     8341 ttgcttcaaa aatatcaaat taatgtttta tgttgtacac cgacagaata tcgtatgatg
     8401 gctaaattac agaacttaaa tgattatgac ttaacgcatt tacatagtgc cgtttctgca
     8461 ggtgagccac taaatcgaga agtagttgaa caattcaaga aatactttaa tttaactgta
     8521 agagatggat atggccagac tgaaagtacc ttgcttatag gctttttaaa agatacacca
     8581 caaaggattg gatcaatggg taaaggcatt ccgggtagtt cggtcactgt agtagatgat
     8641 gaggggaata gcgtacctgc taatacgaaa gggaatattg cagtaccact tgatttgcca
     8701 gcacttttca aagggtatta taaagagcca gaacgtacgg cgaaagcgca aacaggtaaa
     8761 tactacatca ctggtgactt agctcatgta gatgatgatg gttatttctg gtttgaagga
     8821 cgtcgagatg acattattat tagttcaggc tatacgattg ggccttttga agttgaagat
     8881 gcgttaacga atcatccagc cgttaaagaa tgtgctgtag tagcaaaacc tcatgatata
     8941 cgaggaaata ttgttaaagc attcgttatt ttacaggacc atactgcggg tgatgatacg
     9001 ctagttaaag aactacaaca atttgttaaa aatgaagttg caccatataa atatccgaga
     9061 gagattgaat tcgttgacga cttaccaaaa acaaactcag gtaaaattcg tcgtgttgaa
     9121 ctacgagatg ctgaaataga aaaatggcaa caacaaaaag atagcaatca ataaacatag
     9181 caaagcagtc caatgcgtaa ggtatgatga tacgcactgg actgcttgtt tatttatgat
     9241 gttctttgtc gtctagttat aaatattaaa attagtttag aaacaagcgc aaccagtatt
     9301 aagaatgaga aaatataaga tgttaaacct cgaatatccc agtttaataa aaacacatta
     9361 ctatcaccgg cattaagttg aaactcagta tgtgttagaa cgataatcac aatgcctata
     9421 agtgcaagat tcaaaataat tttcaatatt aaaggtaatt gtgatgtttt tcttggatgc
     9481 atatattgaa tctccttttt atgattgatt ttacacttat attgtatcaa aatagagtat
     9541 aggtttaaaa tgctataagt caaatgtaga ttaaaacaat cgttagctat ttgttgtcaa
     9601 ataattatga tcgatttgat atttatcttc tatgaataaa agatttaata tattgcaaag
     9661 ttaatgaata attataattt acatttacca ctgcttgctt taacatatac atgtaagcac
     9721 taactatagt attttgttac agcgtatcaa ttaaaacagg aagttatcta tggcgttgat
     9781 attttaaaga aatatgtcag ttttatgtaa ctttcatatt gatgatacac atttacaatt
     9841 tttggagagg tgaaaccaat ggctaagtct aatagtaaag acatcgtttt aattggagcc
     9901 ggtgtactta gcacaacatt tggttcaatg ttaaaagaaa ttgagccaga ctggaatatc
     9961 cacgtttacg aacgcttgga tcgtcctgca attgaaagtt caaacgaaag aaataatgct
    10021 ggtacgggtc atgcagcatt atgtgagttg aactacacag ttttacaacc tgatggttct
    10081 atcgacatcg aaaaagcgaa agtgattaac gaagagtttg agatttcaaa acaattctgg
    10141 ggtcacttag tgaaaagcgg tagcatcgag aacccaagag aatttatcaa tccattacca
    10201 cacatcagtt atgttagagg taaaaacaat gttaaattct taaaagatcg ttacgaagcg
    10261 atgaaagctt tccctatgtt cgataatatc gaatatactg aagacatcga agtaatgaaa
    10321 aaatggattc cattgatgat gaaaggccgt gaagataacc ctggtatcat ggcggcaagt
    10381 aaaattgacg aaggtacaga tgtaaacttc ggtgaattaa cacgtaaaat ggctaaaagc
    10441 attgaagcac acccaaatgc tacagtgcaa tttaaccatg aagttgttga ttttgaacaa
    10501 ttatcaaatg gtcaatggga agttactgtt aaaaatcgcc taactggtga gaaattcaaa
    10561 caagtaactg actatgtatt catcggtgct ggcggtggag caattccatt attacaaaaa
    10621 acaggtatcc ctgaaagtaa acatttaggt ggattcccta tcagtggtca attcttagct
    10681 tgtacaaacc cacaagttat tgaacaacac gatgccaaag tttatggtaa agagccacct
    10741 ggtacaccac caatgactgt acctcattta gatacgcgtt acattgacgg ccaaagaaca
    10801 ttgttatttg gaccatttgc taatgttgga cctaaattct tgaaaaatgg ttctaactta
    10861 gatttattca agtctgttaa aacatacaac attacaactt tattagcagc agcagttaaa
    10921 aacttacctt taattaaata ctcatttgat caagtgatta tgacaaaaga aggttgtatg
    10981 aaccacttac gtactttcta tccagaagca cgtaatgaag attggcaatt atacactgct
    11041 ggtaaacgtg tacaagttat caaagataca cctgaacacg gtaaaggatt catccaattc
    11101 ggtacagaag tggttaactc acaagaccac actgtaatcg cattattagg tgaatcacca
    11161 ggggcttcaa cttcagtttc agttgcgtta gaagtattag aacgtaactt ccctgaatat
    11221 aaaactgaat gggcacctaa aatcaagaaa atgattccat catacggtga atcattaatt
    11281 gaagacgaaa aattaatgag aaaaatccgt aaacaaactt caaaagactt agaattaggt
    11341 tactacgaaa actaaattct aatagctata tttaaaatcg ggcaactgag aaatcttata
    11401 gatttttcag ctgtccgatt tttttcgcgt aatctaatac atacgcaaaa tcaactttaa
    11461 tcaaaaagac tcatacacaa tgcctttaaa gcacatgtat gagtcctttt tagtagttta
    11521 tatcaaaaaa tacgtttaat gtataaatta gtttttgttt acagatgcgt cgtagattga
    11581 ttctacagcg tcacctaatg ctttatcgaa ttcttcttta gattgtccag ctcttaaatc
    11641 actagctaat gcacgtgaga agcttgcgat aagttcagcg ttatctttaa gtaattcgtt
    11701 cgctttttct ctgctgtaac cacctgataa tacaacgata cgaacaacat ttggatgttc
    11761 agctaagtct ttgtataagt tagcttcagt aggaattgtt aattttaaca taactaattg
    11821 atcagcattt aagctgtcta aacctttttt aagctcagct tttaatactt tttcaatgtc
    11881 agctttatct tttgcgttaa tattaacttc tggttcgata attggcacta accctttagc
    11941 aataatttgt ttagcgactt caaattgttg ttcaacaacg tctttgatac cttgttcatt
    12001 taattctaag atgttagaac gcatttttgt accaaaaatg tgacgctcgt ttgcacgatc
    12061 taataagctg tctaagttgt cgattggttt cattaattga acaccatttt gctcttcagc
    12121 aagacctttg tctactttta agaacggaac aacaccttta tcagctaagt aatctgcagt
    12181 gtatttgcct tctacttcgc gatccattgt ttgttcgaaa agaatagcac ccaaaatttt
    12241 atctggtgag aatgaaggtg aagttaccac acgtgtacgc atatcgtgaa caagttggaa
    12301 catttcgtct tcattgctat attgatcttc gtttacacca tattctttaa gtgcttttgg
    12361 tgtactacca ccactttggt ctaatgcggc aataaagcct tttccatttt tcattttttc
    12421 taattgctct ttattcatac tttccactcc ttaacttttc aatacacctc cagtatgata
    12481 aaaatgagaa catttctcaa gtcataaacc ttgaaaagtg tataaaatgt gaaaaataat
    12541 tgtcagttta attaaaaata ttattttatt ctaggtatga ctaacgccat taatgacata
    12601 aagaaaatat gtgtaataat ccaaccgatt aattctgtca cactaaattg aaaaattgga
    12661 cgttgcgcaa taaatactaa aaagggatac aatgctataa ataagaaaac taaagggata
    12721 taacatagat agtaaagcct tttagaagta tgaaacagca acacaaatac aaaataaatg
    12781 acgctaccaa ttagtaagtg acatattatt tctagagtat atggtgtatt gtcagaagga
    12841 attagaaaat ctaaattcaa taataaagct tttaaatgtg attgattgct tattatgtca
    12901 aacaacatgg tcatccatag caacatgaat gatatgaaaa tagcagcaat gaaatggaac
    12961 aatactttta aacgcattca tatcacctca gagtcaaatg gaatatagta aacaattccc
    13021 taatcgaaca aaattatgcg cataaacaaa gtagactgat ataaaattct taattatcag
    13081 aatatattta caaatctgaa ttttattagt atattggata gtattcataa tgacatgacg
    13141 gtattttcac gtgatatcaa atcgaatgtg gttatcgatg tagggaaggt aattatacga
    13201 ataaggtctg tcaattggag gggaatgtat atgagtaaag cacatcaatt aattcaagag
    13261 gatgaacatt attttgcgaa atcaggacgt attaaatatt atccgttagt tattgatcat
    13321 ggatatggag caacattggt tgatattgag gggaagacat atatcgattt gttatcgagt
    13381 gcgagttctc aaaacgtagg tcatgcacct agagaagtaa cagaagcgat aaaggcacaa
    13441 gttgataaat ttattcatta tacaccagca tatatgtatc atgaaccgtt ggtacgttta
    13501 gctaagaagc tttgtgagat tgcacctgga gattttgaaa aaagagtgac cttcggatta
    13561 accggatcag acgcaaatga tggcatcatt aaatttgcca gagcatatac agggcgtcct
    13621 tatatcatta gtttcactaa tgcttatcat ggttcaactt ttggctcgtt gtctatgtca
    13681 gctattagtt taaatatgcg caaacattat ggtccgttat tgaatggttt ttatcatatt
    13741 ccgtttccag ataaatatcg tggtatgtac gagcagccac aagctaattc agtagaagaa
    13801 tatttagcac ccttaaaaga aatgtttgcg aaatatgtac ctgctgacga agtagcatgt
    13861 attgttattg aaacgataca aggcgatggt ggacttttag aaccagttcc agggtatttt
    13921 gaagcgttag aaaagatttg tcgtgaacat ggtattttaa tcgctgtcga tgatattcaa
    13981 caaggttttg ggcgaacggg tacatggagt tcagtctcgc attttaattt tacgcctgat
    14041 ttaatcactt tcggaaaatc tttagcaggt ggtatgccta tgtcagcaat tgttggacgc
    14101 aaagagatta tgaattgttt agaagcacca gcacatttat ttacaacagg tgctaatcca
    14161 gttagttgtg aagctgcatt agccacaatt caaatgattg aagatcagtc gcttcttcag
    14221 gctagtgcgg aaaaagggga atatgttagg aaacgaatga atcaatgggt atctaaatac
    14281 aatagtgtag gcgatgttag aggtaaaggt ctgagcattg gtattgatat tgtttccgac
    14341 aaaaaactca aaacacgtga tgccagtgcg gcacttaaaa tttgtaatta ctgctttgag
    14401 catggcgtag ttattatagc tgtagcagga aatgtgttgc gattccaacc gccattggtg
    14461 ataacatatg agcaattaga cacggcgtta aacactatag aagatgcact gactgctttg
    14521 gaaacaggta acttagatca atatgacata tctggacaag gttggtaata gcgattctct
    14581 taatataaaa taaaaaatca tttccacatc tggatgttaa tcagatggga aatgattttt
    14641 ttgattttta ttttggtggg tggtattcag ctacgtcatt tttcttagaa tgtctaagtc
    14701 cataacttaa atatagaatg ataccaacaa taaaccaaat taaagtgtat aatttcgctt
    14761 cgaatcctaa tccccagaat actagcaata ctaaaacaaa tgtaactgct ggtaatacag
    14821 gatataaagg taatttaaac gcaggaattg gtaaatcttt accttcacgc tttctcaaac
    14881 gatacattgc taatgaaacg aacataaatg caacaagtgt accggctgaa attaattgtg
    14941 ctaaaaatgc gaatgggaac atagaaccaa ttaaaacacc aataatagta agtataacta
    15001 gtgcacgatt aggtaaatgt ttgtcgttta agtggcttaa ccatgaaggt aataagccgt
    15061 cacgtccaaa tgaataaagt aaacgtgagc ctgctaacat cataccaatt aatgctgtaa
    15121 acataccgat aacagagata gcttgaacaa tagctgctac aacaccatga ccactttgac
    15181 gtaaagccca accaacaggt tcagcattgt ttgcgtattg tgagtaatgg aacataccaa
    15241 ctaacacaag tgctacagca ataaataata cgatagcaat acttaaagaa ccaagaatac
    15301 ctctaggcat tgtcttttga gggtcaagtg cttctgctga gtttgctgcg atagaatcga
    15361 aaccgatata cgctaagaaa atcattgaaa caccagcata tatgccttgc catccaccaa
    15421 agtcaccagt agcagttact ttgtgttctg gaataaatgg cacatagtta ctaacattta
    15481 ttgctgttaa acctacgatt acaaataaaa taatagctaa tacttttaaa ataactaaaa
    15541 tattttccat acgtgctgct tccgacatac cacgtgatag taataatgca gttaataaaa
    15601 taacgatagc agcaataata tcgataaaac cgccattcgt accaaatgga tttgataatg
    15661 ctgctggtaa ttcgatgcca attggtttca caagtccgcg taaattcgct gagaatcctg
    15721 atgcaacaaa ggctacggcg ataaaatatt cagctaatag agcccaaccg gcaacccatc
    15781 caaaaaattc accaaataat acattgaccc aagaataggc tgaacctgca aatggcatag
    15841 cggcagccat ttctgcataa gtaaatgcaa ctaaaccagc aacaatagca gcgagtaaga
    15901 atgataacgc aacggccggt cctgcatgtt ctgcagcaac aatgccaggt agcgtaaaga
    15961 tagatgtcga tacaattgtt cctacaccta aagctaagaa atcacgcacc cgaagtgtac
    16021 gctttaaatg accatcttta ttttgataga tagccggatc ctcttttcgt gctattttat
    16081 tgaaaaaact tcccataaac tttcctccca aacattcata aacaattcta tacggtgttt
    16141 tttaatatgt tatatcatag cacaaataat caatattttg tctaaaaatt ctgaaaaatc
    16201 acaactttat gttacgtatt aatgacttgt cttgataaca tccatagatt ttttaa
//
LOCUS       DAHHRR010000050        16188 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.050, whole genome shotgun sequence.
ACCESSION   DAHHRR010000050 DAHHRR010000000
VERSION     DAHHRR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16188
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.050"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(362..1483)
                     /locus_tag="NBI42_001422"
     CDS             complement(362..1483)
                     /locus_tag="NBI42_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HCD1777845.1"
                     /translation="MNTKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGSISAE
                     LFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMLNMFIMVFLSNSLYPDFEKT
                     FFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFATVAFVISFLSFLSLVLPQS
                     IHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVSLASSSF
                     SIERFSYIYIFQFMILISLFGVYWIITENYINHKLTSIGFRLSYTHLLINIALGVINA
                     AIVFSNSNKLNDLFEINMMYISVLVFYIGLWLITPYFHNELTNAKYISYSLGILVMSY
                     IVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            2016..3197
                     /locus_tag="NBI42_001423"
     CDS             2016..3197
                     /locus_tag="NBI42_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCD1777846.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEINEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSEKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            complement(3280..4032)
                     /locus_tag="NBI42_001424"
     CDS             complement(3280..4032)
                     /locus_tag="NBI42_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCD1777847.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVVN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIETFENKDSNGQQHAPGWG
                     LDALKYLIEERSVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(4075..6303)
                     /gene="metE"
                     /locus_tag="NBI42_001425"
     CDS             complement(4075..6303)
                     /gene="metE"
                     /locus_tag="NBI42_001425"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCD1777848.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGNFD
                     KSKILYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQGDNAKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDESYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            complement(6300..8141)
                     /locus_tag="NBI42_001426"
     CDS             complement(6300..8141)
                     /locus_tag="NBI42_001426"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HCD1777849.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAATKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVITRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLDGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKSFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDIVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            complement(8110..9270)
                     /locus_tag="NBI42_001427"
     CDS             complement(8110..9270)
                     /locus_tag="NBI42_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCD1777850.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQKCLGGDVKYD
                     YARSGNPNRELLEEKLARLEHGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKIEQIEDAIQSNTKLIYIETPSNPCFKITDIKAVSNI
                     AKKHRLLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNDEIRE
                     ALYLIQNGTGNMLSAQDSWMLAKHLKTFPIRFKQSVENAQKIVSFLIEQEEISEVYYP
                     GLNSAHLEQAENGGAVIGLRLKDESKAQQFVDALTLPLVSVSLGGIETILSHPATMSH
                     AALPEEVRETRGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            complement(9267..10370)
                     /locus_tag="NBI42_001428"
     CDS             complement(9267..10370)
                     /locus_tag="NBI42_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1777851.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVLNHITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDETLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSAAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     misc_binding    complement(10433..10802)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11031..11876
                     /locus_tag="NBI42_001429"
     CDS             11031..11876
                     /locus_tag="NBI42_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCD1777852.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKQNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAVIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGAKVDISIKKSVGKISFEFESQEDFLRIIEQLN
                     RRYGK"
     gene            12033..12914
                     /locus_tag="NBI42_001430"
     CDS             12033..12914
                     /locus_tag="NBI42_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCD1777853.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIIIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVDENIDNVEKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAFEDTRVILRVSAETIPGEGFSGARIIRKEVQKMFLQEGI
                     KTPQPIMTPFNHSENGV"
     gene            12944..13147
                     /locus_tag="NBI42_001431"
     CDS             12944..13147
                     /locus_tag="NBI42_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCD1777854.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            13159..14256
                     /gene="ychF"
                     /locus_tag="NBI42_001432"
     CDS             13159..14256
                     /gene="ychF"
                     /locus_tag="NBI42_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCD1777855.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGEDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(14342..14533)
                     /locus_tag="NBI42_001433"
     CDS             complement(14342..14533)
                     /locus_tag="NBI42_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777856.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIIYASKIIPFF"
     gene            14777..15073
                     /locus_tag="NBI42_001434"
     CDS             14777..15073
                     /locus_tag="NBI42_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCD1777857.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            15094..15597
                     /gene="ssb"
                     /locus_tag="NBI42_001435"
     CDS             15094..15597
                     /gene="ssb"
                     /locus_tag="NBI42_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1777858.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            15649..15891
                     /gene="rpsR"
                     /locus_tag="NBI42_001436"
     CDS             15649..15891
                     /gene="rpsR"
                     /locus_tag="NBI42_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCD1777859.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
BASE COUNT         5447 a         2498 c         3014 g         5229 t
ORIGIN      
        1 cgattttggt gatgatagta tttgaaataa tatttagagc aggtgataaa tctttacgat
       61 tgtcatctgt tctttttgtt gtggaatgaa atgcggggtg ataaatatag gtgacacatc
      121 tagattgatt tatttgtttt gaggaggtta tgttgtgtgg gaattatttc cttttagata
      181 gtggggatta gagaatatat gttatttatg agtatcattt gatgattgta taggctaacg
      241 atttccttgg aaatatttaa aaacctcgat catgtagcat aactgaagct tgtcacaaag
      301 tataatgtga agttcgacac tttggattca gttcaaatac tttgaccgag gtaaaaacta
      361 tttattcatg tttattacgt agacgttgat ttttaaaata aattagcatg atgcagaagg
      421 ttgcaactga tactgagata atcattacat ggtcgtgacc tttaaataaa aggctgacaa
      481 tataagacat aacgagtatc cctagtgaat atgaaatata ctttgcgttt gtcagttcat
      541 tatggaaata aggcgtaatt aaccataatc caatgtagaa tactaaaacg ctgatgtaca
      601 tcatattaat ttcaaataag tcatttaatt tattactatt actaaaaaca attgctgcat
      661 taataacacc taaagcgata ttgattaaca agtgtgtata tgacaatcga aaaccaatag
      721 atgttaattt atgattaata taattttcag taataatcca atatacaccg aaaagactaa
      781 ttaaaatcat aaattggaat atataaatgt aactaaaacg ttcaatacta aatgatgaag
      841 aagctaaact gacaagcact tcaccaaaga tgataattgt cagtaatgaa aaacgttcta
      901 ctaaatgcat catattaaca ggtgataata cgagatactt ctggaatgga ataagtcctg
      961 ttgctgctat aaatacacct aaaaaaccag gtatatagtg aatgctttga ggtagtacta
     1021 atgataaaaa tgataagaat gaaatcacaa aggctacggt agcaaaggct tggcatgtac
     1081 gcttatcccc ataatctaaa ccggtacgta tatgtaacaa gtattgtaaa ccgatactta
     1141 gatacataat tgcaacgcat aagaagaatg ggaagaatgt tttttcaaag tctggatata
     1201 aactattaga tagaaagacc atgataaaca tgtttaacat cataaacgag acatctttaa
     1261 atgtgacttg accaaagcga tttgtaaaaa atgtttgatg agaccacatt aaccataaaa
     1321 ataaactcat gacgatatac ttgaaaaata attcagctga aatagaacca ttttgtgttg
     1381 ttaaaatcac atgtgcaatt ttttgaatgg catagacgaa aattaaatca aagaacaact
     1441 catggaatcc tgcacgcttt tcagctaaat gttttgtgtt aatgcattaa ccataaaatt
     1501 ttaactcctt taagatgtgt aattaattta ctaagtatac tatgcatcta ttctagtgaa
     1561 aagaggcttt ttatatttta gtatcgacga agggtttgag atagttgata tgaaggcagc
     1621 ttcaaaattg ctaacaatat aggtgatgta ccgataaatg tgaaggacat tatgaaatca
     1681 acagcaacaa agtagttcca taattgactg tacaatgatg atagcgttgc acttaattat
     1741 aatgataatt tgtggtaaat caaagcataa ttttgtacta tagatgagga tgatagagca
     1801 tatttaagag tgtgaaatgt taaagtgaaa ccgtttacgt ttccgattgc ccaaacaaat
     1861 tacatcattg tataatatga tttgttaaat gcataacaag aatgaaaatg taacatacgt
     1921 agcaattggt ttcataaatt ggatgttagt ggcgtattgg ttcattagac gtattagtaa
     1981 taaaattgta tatatcataa ggagatgaat atgacatgac gagagtcgta ttagcagcag
     2041 catacaggac acccattggc gtttttggag gtgcgtttaa agacgtgcca gcctatgatt
     2101 taggtgcgac tttaatagaa catattatta aagagacggg tttgaatcca agtgagatta
     2161 atgaagtcat cattggtaac gtactacaag caggacaagg acaaaatcca gcacgaattg
     2221 ctgctatgaa aggtggcttg ccagaaacag tacctgcatt tacagtgaat aaagtatgtg
     2281 gttctgggtt aaagtcgatt caattagcat atcaatctat tgtgactggc gaaaatgaca
     2341 tcgtgctagc tggcggtatg gagaatatgt ctcaatcacc aatgcttgtc aacaacagtc
     2401 gctttggttt taaaatggga catcaatcaa tggttgatag catggtatat gatggtttaa
     2461 cagatgtatt taatcaatat catatgggta ttactgctga aaatttagta gagcaatatg
     2521 gtatttcaag agaagaacaa gatacatttg ctgtaaactc acaacaaaaa gcagtacgtg
     2581 cacagcaaaa tggtgaattt gatagtgaaa tagttccagt atcgattccc caacgtaaag
     2641 gtgaaccaat cgttgtcact aaggatgaag gtgtacgtga aaatgtatca gttgaaaaat
     2701 taagtcgctt aagaccagct ttcaaaaaag acggtacagt tacagcaggt aatgcatcag
     2761 gaatcaatga tggtgctgcg atgatgttag tcatgtcaga agacaaagct aaagaattaa
     2821 atatcgaacc attggcagtg cttgatggct ttggaagtca tggtgtagat ccttctatta
     2881 tgggtattgc accagttggc gctgttgaaa aggctttaaa acgtagtgaa aaagaattaa
     2941 gcgatattga tgtatttgaa ttaaatgaag catttgcagc acaatcatta gctgtagatc
     3001 gcgaattaaa attacctcct gaaaaggtga atgttaaagg tggcgctatt gcattaggac
     3061 accctattgg tgcatctggt gctagagtat tagtgacatt attgcatcaa ctgaatgatg
     3121 aagtcgaaac tggtttaaca tcattgtgta ttggtggcgg tcaagctatc gctgcagttg
     3181 tatcaaagta taaataataa gaaaacaggt tatcacaaca gtattaatta catgttggca
     3241 taacctgttt ttatttgttt atggatttat tgggtaatat tagtcatttg atggtttaat
     3301 tgcaaatgct ctaacaggga acccaggtgc atcttttggt ttagggctga tagcgtaaat
     3361 gatggcgcca cgagttggta attgatctaa attagttaat aactcgactt ggtatttatc
     3421 ctgaccaaga atataacgtt cgccaactaa atcaccattt tttacaacgt ccacagatgc
     3481 atcggtatcg aatgtttcat gaccaacagc ttcaacactg cgttcttcaa ttaagtactt
     3541 caaagcatct aatccccaac ccggtgcatg ttgttgtccg ttcgaatctt tgttttcaaa
     3601 cgtttcaata ttaggccaac gttttgacca atcggtacga agtgcaacaa aagtgccagg
     3661 ttcaatagta ccatgctctt tttcccatgc ttctatatgc gcacgtgtta cgatgaaatc
     3721 attgttgttc actacttctg ttgaaaagtc taatacaatt aacggcaata ccaattcttt
     3781 taaatcaatg tcttctaaat aacgtttatt ctcgacaaag tgaattggtg catcaatgtg
     3841 agtgccatat tgcgttacaa tattccaacg ttgcacatag aaaccatgat ctttaaccgt
     3901 gaataaagtt gaaacttcgc ctttttcaaa ctcactaaaa cgtggtattt ctggatcaaa
     3961 tgtatgcgtt aaatcaaccc aagttgcttg ttttaaagta tttaattgtt gccataaagg
     4021 atattgtgtc ataaaatcac ccgtttttag tttattatat gataaatgct gcgattattc
     4081 ttggcgttta gctttaacag cattcacaag tacagtcaat gcatctttaa cttcttcttc
     4141 ttttcgcgtt ttcaaaccac agtcagggtt tacccagaat aatgagcggt cgatttgttg
     4201 tagtgagcga ttgattgctg tagtaatttc ttcttttgtt ggaatacgtg gactatgaat
     4261 atcatataca cctagcccaa tacctaaatc ataattaata tcttcaaagt ctttaattaa
     4321 atcaccatgg ctacgagatg tttcaattga aataacatca gcatctaagt catgaatagc
     4381 atgaatgatt tgaccgaatt gagaataaca catatgtgta tggatttgtg tttcatcacg
     4441 aactgaagac gttgcaagtt taaatgataa aacagcatct ttaagatatt gttcgtgata
     4501 ttcagagcgt aatggtaagc cttcacgtaa tgcaggttcg tcaacttgga taactttgat
     4561 tcctgcagct tcaagtgcta atacttcttc gttgattgct aaagcaattt gatcttgaac
     4621 aactttacgt ggtaaatcaa cacgttcaaa tgaccagttt agaattgtta caggtccagt
     4681 taacatacct ttaactggtt tatctgttaa gctttgtgca taaactgttt catcaacagt
     4741 taaaggcgct gtccatttta catcaccata aatgattggt ggttttacgg cacgtgaacc
     4801 atatgattgc acccaaccga atttagttac taagaaacct tgtaattttt ctccgaagaa
     4861 ttcaaccatg tcattacgtt caaattcacc gtgaactaat acatctaagc cgatgtcttc
     4921 ttgaatttta atccatcgag caatttcatt ttttaagaat gtttcatatg attcgtctgt
     4981 aatgcgttta tttttccaat ctgcacggta ttttcgaact tcacggcttt gtgggaatga
     5041 tccaattgtt gttgttggta gatctggtaa gttcaaacgt ttttgttgtt gctcaatacg
     5101 ttgtgcaaat ggtgattgtc ttgaagtacg cacgctttcg aaatcataat ctaagttttt
     5161 gaatgattga ttttggaaac gctcataacg tgcttttaat ttatcatatt ttgcattatc
     5221 accttggttg aataaacgac gtaatgcatc tagttcatct aatttttcag ttgcaaagct
     5281 taagccttcg ccaacacttg tatctaatgt ttcatcatct aaagatactg ggacatgtaa
     5341 taatgaagat gatggttgaa tgacaagctc attggtgtgt gctaacaatt tatcgattaa
     5401 gactttttta gcttcaatat cacttgccca tacattacga ccatcaataa ttccagcata
     5461 taatattttt gatttatcaa aatttccagc ttcaatttgt ttaaggttat agccattatc
     5521 atggacaaag tctaaaccta aaccgccaac aggtaaagaa cttaagaatt taaggtgtgc
     5581 acgttcaaag tatgtttgaa tgactaattt tttagcaaca ccagcttttt cgaaatagtc
     5641 ataagcttca cgtgtaatat tttcatagct ttcactgtcg tctgtaacta agattggctc
     5701 atcaacttga atgtactcag cacctgcatc aattaatgat tcaaacactt ctttataaag
     5761 tggtaataac gttttaactt tttcttcaaa agtttggtga ccgccttttg ataatttaac
     5821 aaaagtaatc ggaccaacaa tgacagggtg agcgttaacg tttaaagatt gagcatattt
     5881 aaagcgatct aataatacat tgcgactcac tttaggctca acattgtccc attcaggtac
     5941 gatgtaatga tagttagtgt taaaccattt tataagtgca cttgcaacat ggtctttatt
     6001 accgcgagca atatcaaata ataagtcatc atcgatagtt ctaccttgga aacgttcagg
     6061 gatgatgttg aataataatg acgtatctaa aatatggtca tataaagaga aatcaccaac
     6121 tgggatgcta tctaagtgat agtacttttg taataataaa ttttctttat gtagatcagt
     6181 taatgtttga tctaattctt ctttagaaat tttctttgcc caataacttt cgatggcttt
     6241 tttccattct ctttttctac ctaatcttgg gaatcctaag tttgatgttt taattgttgt
     6301 cataatattg cctccttgtg agcagtaata gattttgagt atgctgcaag ttctaatgaa
     6361 tcttcgacat tttgaaacgg tgtgataatg tataaaccat taaaatattc atgaacaata
     6421 tcgattaagt cctttgaaag cttaagactt agttctcgtg ttttggcttt atcatcttta
     6481 actgcttcaa attgttgtaa aatttcatct gacatcttga ttcctggcac ttcattatgt
     6541 aaaaagagtg cgtttttgta acttgcgata ggcatgatgc ctatgaaaaa tgatttgttc
     6601 aagtgcttag tggcatggta aatttcaata attttttctt tgctgtacac gggttgtgtt
     6661 ataaaataag acattccgct ttctatcttt ttctctagtc gttttacggc accatctaat
     6721 ttacgaacat tagggttaaa ggcgccagcg atgttgaagt gtgtacgttt cttcagcgca
     6781 tcaccgtcag tgttaatacc ttgattaaat cttagagcga gttcagttaa tcctttagaa
     6841 ttaacatcat agacattggt tgcacctggt aagtgaccaa cttttgaagg atcaccagtt
     6901 atggctaata tttcgttaac gccaatgagc gataatccaa gtaaatggga ttgcaagccg
     6961 attaagtttc ggtctcgaca tgtaatatgt acgagtggtt caatattgta atattgttta
     7021 attaagcttg cagcagcaat gttgctaatt ctgacagttg ctaatgaatt atctgcgagt
     7081 gttaccgcat ctacattagc tttatcaagt ttagcgatat tttcaaaaaa tctatccgtg
     7141 tctaaatgtt tcggtgtatc caattcgata ataacggttg gacgttcttg aaccttagat
     7201 gttaatgatt gtctaacttt attttgagat ggattgaaaa gtgctttcgt tggtatcgga
     7261 atcacttttt tgtcattaac aggtttaagt gtctgaatag attctttaat aaatttgatg
     7321 tgctcgggcg ttgtaccaca gcaaccacca attaaacgaa caccttcgcg aattagattt
     7381 tgagcaactt gaccgaaata ttgtgcattg tcactatact taaattcact attttcaata
     7441 tctagtaagc tagcatttgg ataacaagat aagaatgcgt gctctggtaa ttcaatatgt
     7501 gtgaaagact cttgcatatg gtgcgggcca tgatgacaat tgagtcccac gatgtttgca
     7561 ccacattgaa cgagttgttt taatccttca ttgattgcct gaccattaac taagtaattt
     7621 gtgtttgaag cggttaattg agcaatgatt gggatatcat atttctttct tgtacgtgtg
     7681 attacattcg ttaactcttc caagtcataa tacgtttcga aaagtaaggc atcgacgcct
     7741 tcttcaatta aggtgtctat ttgaatttca gtatgataaa gaatagtttg taagctgata
     7801 tcctcttgtt tgatacctct aaacccacca acagtgccta atatatacgt atctttagtt
     7861 gctgcttttt ttgcgatgcg aacggcggct tgatgtattg ctttaacttt atcttcgaga
     7921 ccgaatcgtt ttaacttttc aaaatttgca ccataagtat tggtttgaat gacatcagca
     7981 ccagcttcta tataagaacg atggatgcgt tcaactttat caggatggct aaggttatat
     8041 gcttctggac aggtatctaa accttcagag tataaaattg ttccaatagc accatcagct
     8101 accaaaacat tatctttcaa ttgtgtgagg aattgactca ttgaatgcct cctttaatgc
     8161 gtatttgatg tcagcaataa gctcatcagg gtcttccaag ccaacactca atcggaataa
     8221 accgaatgta ataccacgag tttctcttac ttcttcaggt aaagctgcat gtgacatcgt
     8281 tgctggatgg gatagtatcg tttcaatacc accaagactc actgagacaa gtggtaatgt
     8341 taaagcatcg acgaattgtt gagctttaga ttcatctttt aaacgtaagc cgataacagc
     8401 accaccattt tcagcttgtt ctaaatgagc agaattgaga ccaggataat aaacttctga
     8461 aatttcttct tgttctatta aaaatgacac gatcttttga gcattttcga ctgattgttt
     8521 aaatctaatc ggaaatgtct ttaaatgttt agctagcatc caactatctt gagcagataa
     8581 catatttcca gtaccgtttt gaattaaata aagtgcctca cgaatttcat cgttattagt
     8641 aataaccgcg ccggcaatga gatcactatg tccacttaaa aatttagtag cactgtgtat
     8701 aacaatgtca gctcctagta ataaaggtga ttgacctaac ggtgtcataa acgtgttatc
     8761 aacggcgact agtagtcgat gctttttagc tatgtttgaa acagctttga tatcggtaat
     8821 tttaaaacag ggattcgatg gtgtttcaat ataaattaat ttagtgttag attgaattgc
     8881 atcttcgatt tgttcaatct ttgttgtatc aacagttgta aactcgatgt tgaaacgatt
     8941 caaaatttgc tcagtgagac ggaatgtacc gccatataca tcatcgggta agatgacatg
     9001 atcaccagat ttgaaagtca aaagtactgc tgaaatagca gcaattcctg atgcaaaagc
     9061 aaaagcgaat tttccgtgtt ctaatcgtgc tagtttctct tctaaaagct cacgattagg
     9121 gttaccactt cgtgcataat catatttaac atcgccacca agacattttt gatggaatgt
     9181 tgaagaatca tagagtggtg ggttagctga atgatattcc acacctctac gccaatcgaa
     9241 tatcacttct gtctcttttg aaagtgtcat acaatctctc caatctgagc tttatctaat
     9301 gcttggatga tatcgcgttc gatgtcttca taattttcaa cgcctagtga taagcggatt
     9361 aaatactcat caatgccacg tttatctttt tcagcatctg gcatatcaac atgtgtttgt
     9421 gtgtaaggga aggtcactaa tgtttcagta cctcctaaac tttctgcaaa aatacaaatg
     9481 tctaaatttt ctaataattt agcggcgcta taggccttgt taagtcttaa actaagcatg
     9541 ccagtttgcc cgctatatag tacttcgtca attgcttgaa gtgactgaca ttttttagca
     9601 agttttcttg cgtttgattg cgcacgctca atgcgtaaat gcaaagtttt aagtccacgt
     9661 aacaacaaat aactatctat tggtgaaagt gttgcgccag tcatgttatg aaaatcaaac
     9721 aactgttgcg cgagtgtttc atctttgacg gttacgacac ctgctagtac atcgttatgt
     9781 ccaccaatat atttcgtagc tgaatgtaag actatatcag caccttctgc taatggtgtt
     9841 gaaagataag gtgttaaaaa agtattgtcg ataattgaca ataagccttt agctttacaa
     9901 agttgatagt acggctttac atcaatggca atcatttgtg gattagatat tggttcaatg
     9961 aacaacgcta ctgttttatc agttatatga tttagaactt gttcataatc tgtaaaatca
    10021 acatatttaa atttgatatc gtattgttgc tcgtaaaatt caaataatct aaatgtgcca
    10081 ccgtataaat cgaatgaaac taaaatttca tcgtgtggtt taaataaatt acatattaat
    10141 tgaatggctg acattccact tgatgtagcg aatgatgcaa taccatgttc gagtttggca
    10201 aaacaggttt caaatgttga gcgtgtagga tttttagtac gtgtgtaatc aaatcctgtc
    10261 gattgtccta gttttggatg tttgtaggca gtagataaat ggattggatt cgctatagca
    10321 ccagttgaat catctgttaa tgtgatttgt gctaactgag tatccttcat attaagacct
    10381 cctgtaagtt aaaataaaaa gcttccgtcc ttcgtacccg aatgaatcgg ataaaaagga
    10441 cgaaagctta tgtttcgcgg taccaccttt atttgttatt ccatcgctga aataacctta
    10501 ttcagtacgc attaaaagta aatatgctta ctgaacaatt atcacaatta aagtcagtaa
    10561 gtaaggatac attaatgtga tatcccatac ttattaacaa aaaatcgtgc gtaaagaatc
    10621 cagtacgcca tttaacatca atgttaatac tgtatcgcta taacgggcga acccgtagac
    10681 acctcatatt ggcatcaaca ctccaaggcc attttcaaac acgctttcaa aatcttctct
    10741 cagctactaa agactctctg tataagcagg gtgtgtttta ctttcctctt tattgtgttt
    10801 acgtttcatt aaactgttat aagatattaa ttagcttaca gagtaaaaaa agatttgtca
    10861 acaattattc agaaaatttt gatttaaaag ttaatttgtt tgtgaaattg taattggtat
    10921 cttgaagttg aaaaatgaat tattttttaa ataaagtgtg gttaatggtt gtctgactca
    10981 tttagaatac ataaaatata tttaactgtt gttatcaaat aaaaagtgat gtgagtgaat
    11041 tgtcaaaaag tgaagatcaa cgtattacta aaacaaaaga tgaacaaatt aagcaaatag
    11101 atatatcgga tatcaaacag aatccgtatc agccccgaaa aactttcgat gaaaatcatt
    11161 taaatgattt ggcagattca attaaacaat atggtatttt gcaaccaatt gtgcttagaa
    11221 aaacagttca aggttattac attgtagttg gtgaaagaag gtttagagct tcgaaaattg
    11281 ctggtttaaa atatgtatca gcggttatca aagatttaac agatgaagat atgatggaac
    11341 tggcggtcat cgaaaattta caacgagaag acttaaatgc gattgaagaa gctgaaagtt
    11401 atcaacgttt gatgacagat ttgaaaatta cacaacaaga agtagcgaag cgattgagta
    11461 agtcgcgccc gtatatagcg aatatgttga ggttattaca tttaccgaaa aagattgctg
    11521 acatggtaaa agatgggcga ctgacaagtg cacatggacg aacgttattg gcaattaaag
    11581 atgaacaaca aatgcttagg ttagcgaaac gggttgttaa agaaaagtgg agtgttagat
    11641 atttagaaaa tcatgttaat gaattaaaaa atgtttcgtc aaagtcggaa acagacaaag
    11701 tagatataac taagcctaaa tttataaagc aacaagaacg acagttgcga gaacagtatg
    11761 gcgcaaaagt agatatatcg ataaaaaaat cagtcggcaa aatctcattt gagtttgaat
    11821 cacaagaaga ttttctaaga ataattgaac aattaaatcg taggtatggt aaatagttac
    11881 acaattttat ataataactc tttgtgcaag tataaataaa ttgtaatcag tgaacatttg
    11941 attctagata tattgagact ttcgtaggtt ggaagtatat aaaaatatga atacaatatt
    12001 gatatatcat atagaaggga gtaacgctta tcatgaatca agtcatgaat attatttcat
    12061 ctctatttga gccattaaca aaaatagaaa catatgaaaa cattgcaact aaaatcgcta
    12121 tgattattat ttatattatc gtagccctca tagttattaa aatactgaat aaaatgattg
    12181 aacagggatt taaaattcaa aataaaagta aaaagagtaa caaaaagcgc tctaaaactt
    12241 taatatctct tgttcaaaat gtagtgaagt atatagtttg gtttatagtt attacgacga
    12301 ttttaagtaa atttggcatt agtgttgaag gtgttattgc aagtgctggt gtcgtaggtt
    12361 tagcagtagg ttttggtgca cagaccattg ttaaagacgt tattactgga tttttcatta
    12421 tatttgaaag ccaatttgat gtaggtgatt atgttaagat aaataacggt ggtacaactg
    12481 tagcagaggg aacagttaaa tcaattggac tccgttcaac acgtattaat acaatatctg
    12541 gagaacttac aatcttgcca aatagtagca tgggtgaaat aacgaattac tcaattacga
    12601 atggaacagc aattgttaaa attccagtat ctgtagatga aaatattgat aatgttgaga
    12661 agaaattaaa caaattattt acatctctgc gtagtaaata ttatttattt gtaagtgatc
    12721 ctgttgtgat tggtattgat gcctttgaag atactagagt aatattaagg gtttcagcag
    12781 aaacaatacc aggtgaaggt ttctctggtg cgcgtattat tcgtaaagaa gtacaaaaaa
    12841 tgtttttaca agaaggtatc aagacaccac aaccaataat gacacctttt aatcatagtg
    12901 aaaacggtgt ttagtagttt ataatacatg gaggtcatat ttaatggcgt caaaatatgg
    12961 aataaatgat atagtagaaa tgaaaaaaca acatgcgtgt ggaacaaacc gttttaagat
    13021 tattagaatg ggtgcagaca taagaattaa atgtgaaaat tgtcaaagaa gtattatgat
    13081 tccacgtcaa acgtttgata aaaaacttaa aaaaatcatc gaatctcatg atgatacaca
    13141 aagataggag aatgattaat ggctttaaca gcaggtatcg ttggattgcc aaacgttggt
    13201 aaatcaacat tatttaatgc aataacaaaa gcaggtgctt tagcagcgaa ctatccattc
    13261 gctacgattg atcctaatgt agggatagta gaagtgccag atgcaagact acttaaatta
    13321 gaagaaatgg ttcaacctaa aaaaacatta cctactacat ttgaatttac agatattgct
    13381 ggaattgtta aaggtgcttc taaaggtgaa ggtttaggta ataaattctt atcacatatt
    13441 agagaagtag atgcaatttg tcaggttgtt cgcgcttttg acgatgataa tgtaacacat
    13501 gtagctggtc gagttgatcc aattgatgac atcgaagtaa tcaatatgga attagtactt
    13561 gcagacttag aatcagttga aaaacgctta cctagaattg aaaaattggc acgtcaaaaa
    13621 gataagactg ctgaaatgga agtacgtatt ttaacaacta ttaaagaagc tttagaaaat
    13681 ggtaaacccg ctcgtagtat tgactttaat gaagaagatc aaaaatgggt gaatcaagcg
    13741 caattactga cttctaaaaa aatgctttat atcgctaatg ttggtgaaga tgaaattggt
    13801 gaagatgata atgataaagt aaaagcgatt cgtgaatatg cagcgcaaga agactctgaa
    13861 gtgattgtta ttagtgcaaa aattgaagaa gaaattgcta cattagatga tgaagataaa
    13921 gaaatgttct tagaagattt aggtatcgaa gaaccaggat tagatcgatt aattagaaca
    13981 acttatgaat tattaggatt atcaacatat tttactgctg gtgtgcaaga agtacgtgct
    14041 tggacattta aacaaggtat gactgcacct caatgtgctg gtatcattca tactgatttt
    14101 gaacgtggat ttatccgtgc cgaagtaaca agttatgatg actatgtaca atatggtggc
    14161 gaaagtggcg ctaaagaagc gggcagacaa cgattagaag gtaaagaata tattatgcaa
    14221 gatggcgata tcgttcattt cagatttaat gtataaacga tagagtgaag ttaattaaat
    14281 agtatatatg tagaagaggc ggaatcaatt gttcgcctct tttaattatg cgtataattt
    14341 attaaaagaa tggaataatt ttactcgcgt aaataatatc ttgagtgctg aaaaattgtt
    14401 tgccttcgcc agtataagca ggctctaaaa caagattagc ctttgcacaa taaagccatt
    14461 caggatgaat gccactatta agtatctctt ggaattcttg aaaatcttta gaccaatcaa
    14521 tatttaaatt cattccttac cacctcacaa ctattataga acatgcgttc gcttttgtga
    14581 agtgtatttt taaaaatatc atagaaaatt tcaaatgaat taatgtcaaa aatgatatga
    14641 agagtagttt gaattattat tgtaaaaagt aatggcgcat gatataattc tttattgtga
    14701 gtaatgaaaa ttattccttg cttatctgtt ttaagattga taagccgtat agaccacaag
    14761 gaggtgcaaa tataaaatga gaacatatga agttatgtac atcgtacgcc caaacattga
    14821 ggaagatgct aaaaaagcgt tagttgaacg tttcaacggt atcttagcta ctgaaggtgc
    14881 agaagtttta gaagcaaaag actggggtaa acgtcgccta gcttatgaaa tcaatgattt
    14941 caaagatggc ttctacaaca tcgtacgtgt taaatctgat aacaacaaag ctactgacga
    15001 attccaacgt ctagctaaaa tcagtgacga tatcattcgt tacatggtta ttcgtgaaga
    15061 cgaagacaag taataattag agggggcgtt taaatgctaa atagagttgt attagtaggt
    15121 cgtttaacga aagatccgga atacagaacc actccctcag gtgtgagtgt agcgacattc
    15181 actcttgcag taaatcgtac gttcacgaat gctcaagggg agcgcgaagc agattttatt
    15241 aactgtgttg tttttagaag acaagcagat aatgtaaata actatttatc taaaggtagt
    15301 ttagctggtg tagatggtcg cttacaatcc cgtaattatg aaaatcaaga aggtcgtcgt
    15361 gtgtttgtta ctgaagttgt gtgtgatagc gttcaattcc ttgaacctaa aaatgcgcaa
    15421 caaaatggtg gccaacgtca acaaaatgaa ttccaagatt acggtcaagg attcggtggt
    15481 caacaatcag gacaaaacaa ttcgtacaat aattcatcaa acacgaaaca atctgataat
    15541 ccatttgcaa atgcaaacgg accgattgat ataagtgatg atgacttacc attctaataa
    15601 aaattaacga aattaaagcg aaaaaattat caaaggaggc acacaatcat ggcaggtgga
    15661 ccaagaagag gcggacgtcg tcgtaaaaaa gtatgctatt tcacagcaaa tggtattaca
    15721 catatcgact acaaagacac tgaattatta aaacgtttta tctcagaacg cggtaaaatt
    15781 ttaccacgtc gtgtaactgg tacttcagct aaatatcaac gtatgttgac tacagctatc
    15841 aaacgttctc gtcatatggc attattacca tatgttaaag aagaacaata atatttaata
    15901 tattataacc ctcgtaggca taggcttatg ggggattttt ttatgcttta ggatatagaa
    15961 aagggacaga aaagggtaaa aaagaggatg tgaaagtttt gtgtttggga tttgcacaaa
    16021 gatatgttta tattacaaaa ataatatgaa tttagatgaa taaaaaaaga actacgcatt
    16081 ttaaataaaa tgcatagctc ttctttttct tgcatacgaa ttaaaataac tcgcgagacc
    16141 tataagtctc tttcctcact agatagttta tacttttggt ctgttgaa
//
LOCUS       DAHHRR010000051        15856 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.051, whole genome shotgun sequence.
ACCESSION   DAHHRR010000051 DAHHRR010000000
VERSION     DAHHRR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15856
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.051"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            172..1038
                     /locus_tag="NBI42_001437"
     CDS             172..1038
                     /locus_tag="NBI42_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD1777860.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            1058..1558
                     /gene="queF"
                     /locus_tag="NBI42_001438"
     CDS             1058..1558
                     /gene="queF"
                     /locus_tag="NBI42_001438"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCD1777861.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            1898..3403
                     /locus_tag="NBI42_001439"
     CDS             1898..3403
                     /locus_tag="NBI42_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCD1777862.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
     gene            3685..4602
                     /locus_tag="NBI42_001440"
     CDS             3685..4602
                     /locus_tag="NBI42_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCD1777863.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTNDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMTSAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLTIDDKTYSGETTMILSANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            complement(5209..5751)
                     /locus_tag="NBI42_001441"
     CDS             complement(5209..5751)
                     /locus_tag="NBI42_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HCD1777864.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPHAQEVVKKLTKHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(5910..6968)
                     /gene="hisC"
                     /locus_tag="NBI42_001442"
     CDS             complement(5910..6968)
                     /gene="hisC"
                     /locus_tag="NBI42_001442"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCD1777865.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEHFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLDVLSNFKYE"
     gene            complement(7208..8722)
                     /locus_tag="NBI42_001443"
     CDS             complement(7208..8722)
                     /locus_tag="NBI42_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCD1777866.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPSALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(8715..9692)
                     /locus_tag="NBI42_001444"
     CDS             complement(8715..9692)
                     /locus_tag="NBI42_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1777867.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQHLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(9914..11695)
                     /gene="recQ"
                     /locus_tag="NBI42_001445"
     CDS             complement(9914..11695)
                     /gene="recQ"
                     /locus_tag="NBI42_001445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCD1777868.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(11707..13590)
                     /locus_tag="NBI42_001446"
     CDS             complement(11707..13590)
                     /locus_tag="NBI42_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCD1777869.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQTVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESINWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LIEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREESEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(13861..15801)
                     /gene="ltaS"
                     /locus_tag="NBI42_001447"
     CDS             complement(13861..15801)
                     /gene="ltaS"
                     /locus_tag="NBI42_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HCD1777870.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKQQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
BASE COUNT         4992 a         2764 c         2295 g         5805 t
ORIGIN      
        1 cacaaacatt cataaaagtg taatgcccta ttattccgac aaataaacac agaacatttg
       61 ttcggtacag tctattatag tctttttata gtaaatttca actatttatg cacaacatgt
      121 tgttttatgc taatataaat acgttaattt tatataaaag aggcgataaa attgaatcct
      181 aaagtaaaag gtatcattgc cattttaatt tcggctattg gtttcagttt tatgtcagtg
      241 tttttcagac ttgcaggtga ccttcctgtt tttcaaaaat cattagcacg aaacttagta
      301 gcaatgttta ttccattatt ttttatatat aaataccatc agccaatgtt tggcagactt
      361 tcaagtcaac ccttattaat aacacgatca acacttggac ttattggtgt attgttaaat
      421 atttatgcca ttgatcatat ggtacttagt gatgccgatt cattaatgaa actcaatcct
      481 ttctggacga ttttattaag cattgttttc ttacatgaaa aggtaaggaa gtatcaaata
      541 acagcaatga taattgctat tttaggtatg cttttaatag tgaaacctga attttcatca
      601 tctatgattc catcattagc tggcttattc tcaggtattt ttgcagcatc agcatataca
      661 tgtgttcgag cacttagtac aagagaagca ccgtatacta tagtatttta cttctcctta
      721 ttttctgtca tcgttttaat acctttcact gcatatactt atgaaccaat gagtcagatg
      781 caaatacttt atctattagg ggctggctta gctgccgctg tgggtcaaat tggtgttaca
      841 ttagcatata gttttgcagc agcaaaagat atttctattt tcacgtatgc atctataatt
      901 ttcacagcaa tacttggatt tattctattc ggagaatctc cagatttcta tgcaacatta
      961 ggttacgtcg ttattatcgg tgcaagttat tacatgtttg aaaaagcacg ccgagacgcc
     1021 aaaataataa aaaaataaat ttaaaggagc gctttatatg gcacatggcc gtcaacaaga
     1081 tgaattacaa gatataactt tattaggaaa tcaagacaat acttacaatt ttgactaccg
     1141 tcctgatgta ttagaatcgt ttgataataa gcatcaaggt cgtgactatt tcgtaaaatt
     1201 caactgtcca gaatttacat cattatgtcc gattactggg caaccagatt ttgcaactat
     1261 ctatatttca tatattccaa atgttaaaat ggttgaatca aaatctttga aattatactt
     1321 atttagtttc agaaatcacg gtgatttcca cgaagattgt atgaatatta ttatgaatga
     1381 tttgatagag cttatggacc cacattatat tgaagtctgg ggcaagttca caccacgtgg
     1441 tggaatttct attgatcctt atacaaacta tggacgtcca aattctaaat atgaaaaaat
     1501 ggctgagcat cgtttaatga atcatgattt atatcccgaa aaaatagata atcgttaaat
     1561 gtattattta ataaacacac caataagttg attttcctaa cttattggtg tgtttttcat
     1621 ttagcataca aaataggtta cattaaaata acattttata ccaaagtaca ccaaaagaat
     1681 attagtacac gaattaaaca acatttttat agaaacctat tgcactttaa cgccaataag
     1741 tatattttta tattatctct aattaattgt gcgctcttga taacagaata ttctcaatat
     1801 ttttattttt ttgtgatttg tggaatattt agttgataag gcacaatcaa atttacttaa
     1861 actattgtat taggggaaga aaggatggga tgtataaatg acacaacaaa actcccatgg
     1921 aaatcaaatt caagacatac ctcaaacagg atttttcggg catcctcgag gactaggcgt
     1981 actcttcttt gtagagttct gggaaaggtt tagttattat ggcatgcgtg ccctactcat
     2041 tttctacatg tactttgccg taacagataa tggccttgga attgataaaa caacagctat
     2101 gtcaattatg tcagtttatg gttcattaat ctatatgaca tcaataccag gcggatggat
     2161 tgctgacaga attacgggca ctagaggcgc tactttatta ggtgccgtat ttattattat
     2221 cggacatatt tgtttaagct taccatttgc attaatcggc ttattcacat caatgttctt
     2281 catcattatt ggttcaggtt taatgaagcc aaacatttca aatatcgttg gccgtttata
     2341 tcctgaaaat gatagacgta tggatgcagg ttttgttatt ttctatatgt cagttaatat
     2401 gggtgcatta ttatcaccta ttattttgca acactttgtt aatgttaaaa acttccacgg
     2461 cggattcttg attgcagcag ttggtatggc attaggttta gtatggtatg tacttttcaa
     2521 ccgcaaaaac ttaggtagcg ttggtatgaa acctactaac ccattgacac cagctgaaaa
     2581 gaaaaagtat ggtcttatta tcggaagtgt tgtcttagca attgtattaa ttatcgttat
     2641 tggtgcatta actaactcat tatcatttaa cttagttagt aatactgttt tagtattagg
     2701 tatcgcatta ccaatcattt acttcacttt aattattaga agtaaagatg tcactgatac
     2761 tgaacgttct cgtgttaaag catttattcc attatttatt cttggaatgg tgttctgggc
     2821 tattcaagaa caagggtcta atgtattaaa catttatgga attgaacatt cagatatgaa
     2881 attaaactta tttggttgga aaacaaactt tggtgaagcg atcttccaat caattaaccc
     2941 attatttatt ttattattag ctccaattat ttcactttta tggcaaaagc ttggaactaa
     3001 acaacctagc ctgccagtaa aatttgcaat tggtacgttc ttagcaggtg cgtcatacat
     3061 actaattggt attgtaggtt atgcatcagg ttcatcaaac ttctcagtta actgggttat
     3121 tttatcgtac attatttgtg ttatcggtga gctttgctta tcaccaacgg gtaatagtgc
     3181 tgctgttaag ttagcaccta aagcatttaa tgcccaaatg atgagtattt ggtacttaac
     3241 taacgcttct gcacaagcaa tcaatggtac tttagttaag ttaatagaac cactaggtca
     3301 aacaaattac tttattttcc taggtgttgt tgcaattatt gttacaacaa ttgtattagc
     3361 attctcacct ttaatcatca aagcgatgaa aggtatacgt taattttgtt ggcctaattc
     3421 aaaaaacagt aagtcattta aatggcttac tgttttttta taggtttcta ttaattaaat
     3481 tcaagatatc agtataaata aaagcttaat atgctcgtta tagacaacta aacaatctaa
     3541 ttagtaatta tattttcaca ttccactata gacatcaatg ttagttatta ccattttctc
     3601 tatttaaaaa catattttgg gtattaacaa ttaaaagtgg gtatatatat ttaatgaacc
     3661 aatttttaga aagtaggcta aactatggaa aataaatata cacatggtgt cctcttttac
     3721 catgaacaca gtggattgaa aaatattaat caaggtattg gggaagttac aacagcatta
     3781 agttcaatat gtaagcatct ttctattcaa ttgagtgaaa acgaaggtga cattattaaa
     3841 tattgccaag aaatcaaaac taaaaactat gcaaaagatg tagatatatt atttattctt
     3901 ggcggtgatg gcacggttaa cgaacttata aatggtgtta tgacaaatga ccttcaactc
     3961 cctattggaa ttttaccagg cggtactttt aatgatttta caaaaacgct aaatatagca
     4021 cctaatcata aacaagctag tgaacaaatg acttctgcac aagttggtac atacgatgta
     4081 attaaaataa ataatcaata tgcactcaac tttgttggct taggtctaat tgttcaaaac
     4141 gctgaaaacg tacaagatgg ttcaaaagat atatttggta aattgagtta tattggatca
     4201 actgtcaaaa cactgctaaa tccaacacaa tttaattatc agttaacaat tgatgataag
     4261 acatattctg gcgaaacgac catgatacta tcagcaaatg gtccattcat tggtggtagt
     4321 cgtataccat tgactgattt gtctcctcaa gatggtgaac tgaacacttt catttttaat
     4381 gaacaaagtt tcagtatttt aaatgatatt tttaaaaagc gtgatagcat gaattggaat
     4441 gaaattactc aaggtattga gcatatacct ggtaaaaaga tttcacttac aacagatcca
     4501 gcaatgaaag ttgatattga tggcgaaatt tcattagaaa cacctattga tattgaggtt
     4561 ataccaaatg ccattcagtt acttactgtc aatgatttgt aagcatccgg aatgatctca
     4621 acgggatatt atattaggtt taaagtgtaa tcataagatt caacgtacat tatattcgtt
     4681 gttattaact ttaagctatt accctgtttc aaaaacagac atgtaatttt ggcgcatgcc
     4741 actaaaatga ttgatgttat acttttaatc atagtggttt atgtgccttt ttcaaattct
     4801 ataaaaaatc ggatgacgtg taatctgaca tagattaaca cattcatccg atttataata
     4861 atgagataga cttacattta ttgagagtga gacggaaatg ataaagaacg actaatggtt
     4921 gattatgtag cgattcttta tcattagtca cagctaatgt gtacttaaaa ataggattgc
     4981 atgagttaca ctcatgcata agaaatacta atttctaaag aaaaagtatt tctttatgtt
     5041 ggggccccgc caacttgctc tgcctgtaga aattggcgtt ccaatttctc tgtgttgggg
     5101 cccgccccaa ctcgcattgt ttgtagaatt tcttttcgaa attctctttg ttggggcccc
     5161 agtatatgca ttttatttgc tacatgaaat gcttcaagtg atttattttt atttctcaat
     5221 attatctaaa aaatactgtt ctacatcttt ccaactattt acgcgttcaa atcgatcatc
     5281 attaatatta tgcactgctg taaacataat cggtgtacca gtaaaaattt caagctgtct
     5341 aggattgtca tctattaaat aatcagcttt aacgatgttt tttctaccac aaaaaacaaa
     5401 atgctgagga tctaaaaatg gaaagaactc tagtaaccat tcatatttat cactaaatga
     5461 tgttggtaca tccattgctg ctgtagcaat atatacatca taatgtttag ttaatttttt
     5521 cacaacttct tgtgcatgcg gcatcacttt aagatgtctg aagaagcctg gttctctcaa
     5581 tacttctgta attaatccat catgttcggg aataacatgt ttaagttttt gaccattcaa
     5641 agcttccatt ttaataccta aatccgctct aaaattgaca gcatcaatga tttctcctaa
     5701 tgtatctgcc aatacttcat ccatatcaat cgcgattgat ttacgggtca ttttaatctt
     5761 ctcctcaaaa tatcagtatt gattcagtat agcaaaaaag caattgatac aaaacaatat
     5821 catctattgt tgctttttac actgaatact ctcacctcat catttacact gcatttttgt
     5881 cacattttta tacaatgtga caacacttac tattcgtatt taaagtttga taaaacatct
     5941 aacattttat cattttgttc tttaaaacca attgtaattc taacaccagt tggaaatggt
     6001 cgcgtaatac accctacatt taaaagtgct tcataaagtt catttacccg ctttgttttt
     6061 acaaatataa aattcgtttg acttggtaag aaatgctcgc tttgaggtaa ttgataaaat
     6121 ctttcgcgtt caacactatt tttatgtgtt acctctttta aatattgttg atcttcaaga
     6181 gctgcaactg ctgcgtattc agatatacgt gtcacattaa atggtggtct aatgatgttc
     6241 catttttcaa tcgcatgttc gcttgctacc acatatccta cacgtaaacc cgctaatcca
     6301 tacgcctttg aaaatgtacg taataagaat gcattgtcat atttttgttg caaagcaagt
     6361 gtatccgggt agtcctctgc tgtcacaaat tcaaaataag cttcatctat aattactggt
     6421 acatgtggag gtacttgaga taaaaacgaa tctaagctct catgattaaa atatgtacct
     6481 gtaggattat ttggattaca taaccatacc aatgacgtat cttcattaac ttcttttaaa
     6541 ataccttcta aatcgaagcc accatctttt aaaggtactt gtatcacatt agctgattca
     6601 acaatcgcat tgtgataata ttgaccgaat gtagcttcac ttgtaacaat agtatcccct
     6661 ggcgttaata ctgctctaga aatcattaat ataacttcat ctaatcccgc accaaataaa
     6721 atgcgtgatt gatctacatt taaatgttta ctaatcgccg cttttaatgt cggtgatcct
     6781 gtttcaggat aataatataa ctcatctaag tgtgctgata tcgcttcttt aactttaggc
     6841 gatggtccat acaaattttc atttgatgca agtttatata aatctccttc aatgccatac
     6901 ttttctttca atgcccttgg agataaacca ggctgatatg ctgatagttg attaagttgt
     6961 tctttcataa taaaaacaac ctccttagaa ttaactgaaa ttttaaaaaa cttatattca
     7021 ttatatcgct ctatgtttct aaaatgcaat gctactattt tcacatcata ataacttaca
     7081 ttgtgtttaa catgtcatat tttttaatca ttattgcatg tttaaattga acataattat
     7141 agatttaaat ataacaacgc cacataagat ataatttggt gttacgcatc ttatgtggcg
     7201 ttggtcgtta tttgattaat ccttttgctt ttaaataatc tttcgcaact gtatatgggt
     7261 cttcattttt aactgttact ttatagttca tcatttgcat atcttcatca gatattttgt
     7321 tttctagttt gtttaacggt ttcttaattt ctggatgttt ctttaaaaag ctttctttaa
     7381 ataatggtgc tccttgatat ggtggaaata cgtgcttatc atcttttaac acaaccatat
     7441 catattgttt taattcagcg tcagttgaat atgcatctat taaattaata ttacctttat
     7501 tgatcgcttg atatctcaac ttaggttcca ttgtacgtat attatctaaa tttaaattat
     7561 aagccttttg aacagctttg taaccatctg gacgatcatt aaattccaat gtaaatcctg
     7621 gtttaagttg atctttaacc ttatttaaat caccaattgt acgtatatta tgttgtttag
     7681 caaaatcacg ttttacagct aaagcatacg tattgttata cttcattggt tttaacatag
     7741 tcatttgata tttcttttca agactttgct tagcttgttc ataaactttt ttctcttctt
     7801 ttgacttcaa tggttctttt gttaattcac ctaaaactgt tccagtaaac tctaaatacc
     7861 catctatatc gtcagatttt aaagcattaa ataaaaatgc tgttttgccc ataccatctt
     7921 tcacttctac agtatttttg gtctcttctt ctattaaaat tttatacata tttgtaataa
     7981 tcgaaggctc ggagccaagc tttccagcta acgtaatttt atcacctttt tgtgcaaaca
     8041 taggaatagc gatggccagt ataataatca tcactataac acctaacgtc atcaataact
     8101 ttttataaga taatttagcc ataaatctta aaattaaatc aaatataatt gccaataagg
     8161 ctgatggaat tgcacctaat aatatcaatg atgcattgtt acggtctata cctaataaaa
     8221 ttaaatctcc tagtccgcct gcaccaatta atgctgctag tgttgctgta cctataatta
     8281 ataccatagc cgttcttaca ccagccatta taacaggcat tgctatcgga agttcgactt
     8341 tagttaaacg tctaaatggt ttcataccta tacctttagc cgcttcaatg agtgatggat
     8401 caacttcttt aattccagta tacgtattcc ttaaaattgg taacaacgca tacactacaa
     8461 gtgcgataat tgctggcaca cgaccgatac caaataaagg aatcattaaa cctaataatg
     8521 ccaacgatgg tatggtttga agaattgccg caatattcat tacgatttca gatatcgttt
     8581 tagtcttcgt taataaaata cctaatggta ccgcaatagc agttgcaatc aataatgcga
     8641 taaatgatat ttgaatatgt tctatcattg tcgaaaatag ttgcccctta cgttcactca
     8701 atatgtcgaa aaagttagtc atgttgagct acctcctttt tctgggacaa atatttgaag
     8761 atatctttcc tatcaataac atattgacct acgctatctt cttgcatgac aatgacacgc
     8821 tcgctctctg ataaatgttg atacaatact tcaattggtt gattgtcata aacaattgga
     8881 taagcgctca tagatgtaac ctcatcgatt ggtttcataa tatccaagtc acggataatt
     8941 gcgttctctt caacacatgg cgcatcatct tctaaatgac tacccataaa ttgtttaaca
     9001 aattcacttt gaggattatt tttaaatcct tctggtgtgt caatttgttc aatatgccct
     9061 tcattcaaaa gacaaatctt atcaccaagt ttcatcgcct cttgaatatc atgtgtaaca
     9121 aatatgattg tcttcttaat tttagtttgt aattcaatta aatcatcttg aagtttttct
     9181 cggctgattg ggtctaatgc actaaacggt tcatccatta aaataactgg tggatcagct
     9241 gctaacgcac gtataactcc tacacgttgt cgttgccccc ctgacaattc atcaggtttt
     9301 ctgtttttat atttttcagg ttctaatcca accatttcaa gtaattcatc tactctttta
     9361 tctatatctt tttctttcca ctttttcatt tgtggcactt gtgcaatatt ttctttgatt
     9421 gtcatatgtg ggaataatgc aatctgctgc aatacgtatc caatatccca acgcatttcg
     9481 tatactggat aatcacttat tggtttatct ttaaaataaa tataaccttc acttaagtga
     9541 atgagtcgat taatcatttt taatgtcgta gtttttccac aacctgaagg tccaattagc
     9601 acaaaaaatt caccctcatt aatattgaaa ctaatgttat cgacagcaac atgtttgcca
     9661 taacgcttag ttacattttt aaacttaatc actttgccac ctcttttttt ctcatagcat
     9721 aaaaccgaga ttatatgtat gtattcccta tttaaccacg tttattacaa ttttcaaatt
     9781 taaatgattt atccttgaac ttttttaaca aaataatgaa taataggtaa tctccagtta
     9841 agaaatagtg ttattttacc ttaaattcaa aaaaacaccc agtaaaacaa ggaatgctta
     9901 ctaggtgtct tcactatact ttggctttat aattttgaat cgtttctaaa aatgctggac
     9961 aataatgttt taatttgtaa ctacctacgc catcaatatt aatcatatct tgtttcgaag
    10021 caggcttacg tttagcaaat tcctccaacg tgtaatcaga aaatatactt acaggtgcta
    10081 tcgttaattt gtcacttaac tttttacgaa cttctaccaa ctgactgaat aatactcggt
    10141 caaccccttc aaccgtattt ataaatactt tttcagtcgc tttttgctta aatggtgttg
    10201 tgaatacttc tacttcatta ctgagtaatt ttttaattga agtatcacac attaatattt
    10261 catcattttc atttaagaac cctttgaatc ttaattcatc tattaagtga cttaattctg
    10321 atgttgtgta acctttcatt aaaccatggg ttgaaatttg atcataacct ttatacttaa
    10381 tataatctgt tgactctcct cttagcactt gaatgataac actataactc tcttgttgtt
    10441 tcatacgagc gatgcaacta ataatcatct tagcttcttg tgtcatatta tatgatttat
    10501 cttgttgaac acaattacta cattgttcac attcttctaa tttttcattc ggttcaaaat
    10561 aatggacaat tgttgcttct aaacattttt ttgtttttgt atattgaatc attttagtta
    10621 acttttcgcc cattttatct ttatagtcat catcagcttg agagactgtt ataaaatact
    10681 cgtgtaaatt gatatcgcgt tcgctaaata acaaaataca ttcacttttc aacccatcac
    10741 gacctgcacg acccgcttct tgataataag attctaaatc tccaggcata ttataatgaa
    10801 taacaaagcg tacattggat ttatcaatac ccataccaaa agcatttgta gcaacgacta
    10861 ctttaacacg atcaaataag aaatcattct gcgcttcttc tctttcttta ttgctcaaac
    10921 ctgcatgata tataacactt tcaattttct ggctttctaa ggcttcttga agctcttcaa
    10981 cttgcttacg tgtagaacaa taaataatac ctgcatcttc atcgtgtgtt ttaatataat
    11041 ccaatataaa tttttgacgt tgatatgtag gatttacttt aaaaattaag tttctacgct
    11101 tagtactcgt tttaatttga tcagtttgag cgatatttaa cttttcccta atatcttgct
    11161 gtacttcaac cgtggcagtt gctgtcaacg ctattattgt aaaatcttga ggtaacgtaa
    11221 atactttaga aatgacgttt tgataactcg gtctgaaatc atgtccccat ttagatatac
    11281 aatgcgcttc atcaaacgcg actaagtgaa tctttatacg ctgaagcata tttaaaaaat
    11341 atcggttttc aaatctttct ggtgcaacat acaaaaattg aatttctcca tttgataatg
    11401 ctttttcaat acgttgttgc tctttttgag tcaaactact atttaaaaaa gcagcttgaa
    11461 ttcccatcgc ttttaattga tccacttgat ctttcattaa tgatattagt ggacttatta
    11521 caattgttgt accacctaac aataaacctg gtacttgata gcatatagac ttacctccac
    11581 cagttggtaa gacaccaagc acattacgat ggtctaatac tttgctaata atttcttctt
    11641 gtcctggtcg aaacgtttca tacccaaagt aatgcgataa tgtttgttgc atcataaatt
    11701 gaccccttat tgttgttctt taatttcttc taactcactc catcttgtga tgtctaaatc
    11761 atattgaatt tcaagttgtt ctttttcttc gtttagttct ttaattttcc cataatctgc
    11821 acttgcctca atcatgagca catcaatttc ttccattctt acttccgctt gttctatgcg
    11881 tttcatcaat tgttcatatt ctaatttttc tttatatgat aacccatttt tcttacgtac
    11941 agttgtagaa gatttagatt gttgcttcaa tgtggattta tttttatcta atgatttttt
    12001 ataactttca taatcttcaa aagttccgat aatcttttcc atctgaccat catgaataaa
    12061 ccaatatgac tgtgcaactt tatttaagaa gtagcgatca tggcttacgg taatcactgt
    12121 accaccgaaa gtatgaatat aatcttcaag tattgttaaa gtctcagtat ctaaatcatt
    12181 tgtcggttca tctaacaaca gaacatttgg ctggtgtacg agtagacgta gtaaatacaa
    12241 acgcttttgc tctccaccag ataatttata aactttttta ccatgagttg cacttggaaa
    12301 taaaaatcgt tcaagaagtt gtgtaataga taccgaggtt ccatcttttt cttttgcgat
    12361 ctcactttct tctcttaaat aatcaatcat acgaatatct ctatccaggg tctcatcagt
    12421 ttgcttaaaa taagctactt taaccgtctg cccagtcttc aatttacctt cgaattgttg
    12481 gtcttctcca ctcaaaatat ttaagagtgt tgttttacca gctccatttg gcccaacaac
    12541 accaatacgc tcaccttttt gaataatttc cgtcagatgt tcaattaata ctttatcatt
    12601 aatagccttt gataagtctt ctaattcgaa cacttgctta cctaatcttg aataagcaag
    12661 attcaattca cctttatcgt ctttatattg ctggttaact tcattttcta ggtcattaaa
    12721 tcgattaatt ctagcttgtt gctttgtagt acgagcctta gctccagccc tcatccaagc
    12781 aagttcttcc ttatataaag ctcgttgctt ttgttgttgc ttttgaagtg ttacttctct
    12841 ttcagcgcgc atttcaatat aagattcata gttaccagga tatgacgcta acttacctct
    12901 gtttagttca ataattctag tggaaacttc atttaaaaaa tatcgatcat gggttacgaa
    12961 taaaactgta tgaggatatt gcttcacata attgatcaac cagttgattg attcaaagtc
    13021 taaatggttc gtaggttcat ctaataacaa taaatctggt tgttctatta atgttttagc
    13081 aagtacaaca cgtttttgtt gtccgccgga taattcttta atgtatttag tagtatcatg
    13141 tatacctagt tttgagagga ttgttttaat ttcagcgtta tagtcccaag catcatgttg
    13201 atccatagca tcttgcgcat ccatcattcg cttgaacaat ttatcacttt ggtcatccgc
    13261 ataagcttgt actgtctgct catattgctt gatgatgcgt aaagttgttg tatcagaact
    13321 taatactgca tcaaaaacag tcatatcttc attaaggtcc tgtttctgag acgaatatcg
    13381 aattcgatat tgatttggat gcataacatt ggctgtaaaa tcatcatcaa taccaccaat
    13441 tacttttaac aacgtacttt tccctgtacc atttatgcct accaaaccta ttttttcacc
    13501 ttctgaaatt gataaatcta ggttatcgaa tatagtctta tcggcataag atttatttaa
    13561 atgttcaatt ttatatgctt ccatacccat cttccttctt ctgttgaaat ctctacgtac
    13621 ttaaatcagt tctatacatt atacaccttt ttgaaatcaa aagaaatcga taccgttaag
    13681 tgtcataatt taatatattg ttgcttcaac tataaataaa aattgataag gctttgatga
    13741 ctatgctaat gatctatata atataccttt ttatgaacac atataattta aaacataata
    13801 aaaaagaaca taataagaac ccggattgat attttatcaa tccgagttcg tgtttagata
    13861 ttatttttta gagtttgctt taggtcctgt ttcatattta tacttcgaag gatttacctt
    13921 tttgaagtct ggatttttgt agaatctaaa caaatcacca ttaagcacgt tgtcactcat
    13981 ttcgagatcc ttttcaactt gctttttatt cttttcgaaa tcagctggtt gagtagttat
    14041 gagttcatta tttttattag aataaatctt accattaaca tatttataat cttttgttat
    14101 aaagtcacca tttctgaatg gaactacttg attatgacct ttagagaata aatcagtacc
    14161 gaacattaaa tagttcttcg tatctatacc agccaaatgt aaaattgttg gcattacatc
    14221 gacttgacca gcatattcat tattgatacc accagattta ccagggattt taatccagaa
    14281 accagttctg tttaaatctg taaatttagc tggtgtgatt ttttcaccta atagtttttc
    14341 catggcattg ttatggtttt cagagatacc atagtggtca ccataaatca taatcactga
    14401 attgtcatat aatccttttt tcttcaagtc attaatatat tcttctaatg cttcgtctaa
    14461 ataacgcgct gtttgaatat aaccatcaac tgttgcatca cctgtgtttg acttctcaat
    14521 agttgcatcc ttttcatcta atgtgaatgg atagtggtta gtcaatgtaa ttaaatgaga
    14581 atagaatggt gatttcatct tagcttgata attagcagaa tctttaaaga aaattttgtc
    14641 tttcaagcct aagtttacaa cgtttttatc tgacatgtca tagtatgttg catcatagaa
    14701 tttatcgata ccaaagtgtt tatatacttg gtctctgttc cagaatgttt tatagtcacc
    14761 gtgcatgaca tcagatttgt agccttgctt ttgatctaaa attgctggta atgactgata
    14821 cgtattatct ccttttaatg aaaaggcaga accttgcggt aaaccgtata aactgttatc
    14881 cattgtaaat tcagagtcag atgttttacc ttgacctgtt tgatggaaaa agttagggaa
    14941 gtatgtgaat tgctgtttcc cacttgataa tttgtttaaa aacggtgtta cttctttacc
    15001 attaaccttt ttattaatta agaaggtttg gaaactttct aaatgaatct taataatatt
    15061 tttcttcttt gccaccccat aatattctgg gttaggctct gtttgacgtt gtttcgtgta
    15121 atttaatact tttgttaagt catcttcaga tgctaacgct ttttgttgat tattttcgat
    15181 agttttaaca ccatcgtata ctgtaaagtt atacggtcct aaatatttca ctaaatattt
    15241 atggtcaaat gtacgtgtta ataattctgg tctatcagtt tcagcaaaag ctaagtttaa
    15301 gaagaataat gctactgaag ccgccattac gacaggaaca aatttcttac taaatgcttt
    15361 tgtgtctaac cattttgttt taaatattaa aatgaataag taaactaacg tatcaatgaa
    15421 ataaacaaag tcataccatt tgaatgatgc actaaccgca ccacccatag attctacgtt
    15481 acctacttgg tttaaagtac taaacgttaa aaaatccgag aagaatctaa agtacacaac
    15541 attggcatat aataggaacg tcaataagaa gccgcctatg aacatgaacc aaaatgcttt
    15601 tttgccttta aagaatagga acacacttaa aaccagtgct actaaactat aaggattcat
    15661 caataatatt aagttttgta ctaaaccttt aacacctaaa gaaaaatcaa cataataaga
    15721 aaaatacgtc ttcaaggtaa tcgttattac ggttaataag aagaacgcaa aaagactaat
    15781 tttctttttt tgtgaactca tgattctttc ccccgttatt tagataataa atcttgcgaa
    15841 agtacttgaa taaatt
//
LOCUS       DAHHRR010000052        15855 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.052, whole genome shotgun sequence.
ACCESSION   DAHHRR010000052 DAHHRR010000000
VERSION     DAHHRR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15855
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.052"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..1282
                     /locus_tag="NBI42_001448"
     CDS             <1..1282
                     /locus_tag="NBI42_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCD1777871.1"
                     /translation="VIRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEY
                     AHAKNRDTLHLIIFNIFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTY
                     AESIMDSVLKTSFFNKNILSHSFNGKKSLLKNRLINIKEADLKKQSKLILIFICIFTF
                     FIMIIQSQFLMGQSLTDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYN
                     EKESRKRYSPDSTYKIYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQ
                     NSVNWYFERISNQIPKNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKN
                     MMEQNNHFSKKAKNQLSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDK
                     YYFATHLSDGNPSGKNAELISEKILKGMGVLNGQ"
     gene            1272..1652
                     /gene="blaI"
                     /locus_tag="NBI42_001449"
     CDS             1272..1652
                     /gene="blaI"
                     /locus_tag="NBI42_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCD1777872.1"
                     /translation="MANKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            1916..2509
                     /locus_tag="NBI42_001450"
     CDS             1916..2509
                     /locus_tag="NBI42_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1777873.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
     gene            2481..3924
                     /locus_tag="NBI42_001451"
                     /pseudo
     CDS             2481..3924
                     /locus_tag="NBI42_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            3917..4732
                     /locus_tag="NBI42_001452"
     CDS             3917..4732
                     /locus_tag="NBI42_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1777874.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAII
                     DLGQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMI
                     FIGMPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDY
                     EAITTIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
     gene            complement(4852..5826)
                     /locus_tag="NBI42_001453"
     CDS             complement(4852..5826)
                     /locus_tag="NBI42_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCD1777875.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFN
                     SSSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFE
                     MNKASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENS
                     GWIRVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIE
                     DIENYLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYE
                     EDLKLLQKNYKTTLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
     gene            complement(6038..6130)
                     /locus_tag="NBI42_001454"
     CDS             complement(6038..6130)
                     /locus_tag="NBI42_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD1777876.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(6299..6646)
                     /locus_tag="NBI42_001455"
     CDS             complement(6299..6646)
                     /locus_tag="NBI42_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1777877.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNQEGF"
     gene            complement(6665..7282)
                     /gene="cadD"
                     /locus_tag="NBI42_001456"
     CDS             complement(6665..7282)
                     /gene="cadD"
                     /locus_tag="NBI42_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HCD1777878.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRNIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
     gene            complement(8786..9462)
                     /locus_tag="NBI42_001457"
                     /pseudo
     CDS             complement(8786..9462)
                     /locus_tag="NBI42_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            9873..10616
                     /locus_tag="NBI42_001458"
     CDS             9873..10616
                     /locus_tag="NBI42_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCD1777879.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NARQNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSA
                     NPNDNDSDIEEKSEETEPKKGFWSRLFGN"
     gene            complement(10835..11020)
                     /locus_tag="NBI42_001459"
     CDS             complement(10835..11020)
                     /locus_tag="NBI42_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777880.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHI
                     IRVKDGLLGWRKEIVTE"
     gene            11169..11240
                     /locus_tag="NBI42_001460"
     CDS             11169..11240
                     /locus_tag="NBI42_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD1777881.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
     gene            complement(11479..11820)
                     /locus_tag="NBI42_001461"
     CDS             complement(11479..11820)
                     /locus_tag="NBI42_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1777882.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSL
                     DILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAG
                     FLVWAIHSFMG"
     gene            complement(11907..12767)
                     /locus_tag="NBI42_001462"
     CDS             complement(11907..12767)
                     /locus_tag="NBI42_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCD1777883.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLR
                     LSFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIH
                     KREQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLK
                     IHIDDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEF
                     TFDAEKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKERED
                     FLKQLELNWE"
     gene            complement(13151..14419)
                     /locus_tag="NBI42_001463"
                     /pseudo
     CDS             complement(13151..14419)
                     /locus_tag="NBI42_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331753.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(14657..15283)
                     /locus_tag="NBI42_001464"
     CDS             complement(14657..15283)
                     /locus_tag="NBI42_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1777884.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLL
                     NIIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFK
                     KNKKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTG
                     SLDTKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
     gene            complement(15295..15606)
                     /locus_tag="NBI42_001465"
     CDS             complement(15295..15606)
                     /locus_tag="NBI42_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD1777885.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVP
                     KDTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFL
                     K"
     gene            complement(15610..>15855)
                     /locus_tag="NBI42_001466"
     CDS             complement(15610..>15855)
                     /locus_tag="NBI42_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCD1777886.1"
                     /translation="SWNKKQLLLRKIHGYSLFSSNVRYLTISILLSIMLAYTTHILFG
                     SKILLFIIMSIAIIQHLLQIAYIKYLEKYFKDLMREI"
BASE COUNT         5617 a         2399 c         2162 g         5677 t
ORIGIN      
        1 tgtgattcga aaagcagagg ctattcaatc tccaataact ttttggtatg ggaaatatat
       61 tattttgatt cctagttcat attttaaaag tgtaattgat aaaagactaa aatatataat
      121 actgcatgaa tatgctcatg ctaaaaatag agatacttta catttaatta tctttaatat
      181 cttctctatt attatgagtt acaatccatt agtacatatt gtaaaaagaa agataattca
      241 tgacaatgaa gtagaagccg atagatttgt ccttaataat attaacaaaa atgaatttaa
      301 aacttatgcg gaatctatta tggactccgt attaaaaacc tcatttttca acaaaaatat
      361 attaagccat tcatttaatg gtaaaaaatc attactcaaa aatagattaa ttaatataaa
      421 agaagccgat ctgaaaaagc agtcaaaatt aattctaatt tttatttgta tttttacttt
      481 ttttatcatg ataattcaaa gccagttttt aatggggcaa tccttaactg attataatta
      541 taaaaagccc ttacaaagcg attaccaaat tttagatgaa agtaaaaatt ttggttcaaa
      601 tagtggatct ttcgttatgt acagcatgaa gaaagacaaa tactatattt ataatgaaaa
      661 agaaagtaga aaaagatatt ctcctgattc aacttacaaa atttatttag cactgtttgg
      721 acttgaccgt catattataa gtgataaaaa ttcacgtatg tcatggaatc ataaacatta
      781 tcttttcgag tcttggaata aggaacaaga tttaaataca gcaatgcaaa attcagttaa
      841 ttggtacttc gaacgtatta gtaatcaaat accaaagaac tatactgcgg ctcaactcaa
      901 gcaattaaat tatggtaatg aaaatttggg aagttataaa agctattgga tggaagatag
      961 tttgaaaata tctaatcttg aacaagtaat agtttttaaa aatatgatgg aacaaaataa
     1021 ccattttagt aaaaaagcaa agaatcaatt atcttcttca ttattgatta agaaaaatga
     1081 aaagtatgaa ctgtatggga aaacaggtac aggtatagta aacgggaagt ataataatgg
     1141 gtggtttgta ggttacgtaa ttacaaatca tgataagtat tattttgcta cacatttatc
     1201 agatggaaac ccgtctggga aaaatgctga attaataagt gaaaaaatat taaaaggaat
     1261 gggtgtttta aatggccaat aagcaagttg aaatatctat ggctgaatgg gatgttatga
     1321 atataatatg ggataaaaaa tcagtatcag ctaatgaaat tgtagttgaa attcaaaaat
     1381 ataaagaagt tagtgataaa acgattagaa cattaatcac aagactatat aaaaaagaga
     1441 ttataaaacg atacaaatca gaaaatattt atttttactc atcaaatatt aaagaagacg
     1501 atattaaaat gaaaactgct aaaacctttc ttaataaact gtatggaggg gatatgaaaa
     1561 gtttagtgtt gaattttgcg aaaaatgaag aattaaataa caaagaaatt gaagaattgc
     1621 gagatatttt aaatgatatt agtaaaaagt aattagaggt atgtttcaaa cataatacat
     1681 atgtaaacta tgcttgactc ttattaaatt agttttgtta atgacattta aaatatatta
     1741 ttaaagatga gagtgtacag aaaataacca ttttctgtac actcttcttt tatgtctaaa
     1801 taagtaagaa ttacatagga ttatgtgttc aaaaacccaa caaaaaatct atgttcataa
     1861 aagaccttgt aattaagtta tggtacattg aagatgtata taggaggttt gtattttgaa
     1921 aataggttat gcaagagttt caactggatt acaaaattta aatttacagg aggatcgatt
     1981 aaatacatat ggttgtgaaa agatatttaa tgaccatatg agtggttcaa aaagtaaaag
     2041 acctggttta gataaagcaa ttgaatttgc gaggtcagga gatacgattg ttgtttggag
     2101 attagataga ttgggacgta atatggagga tttaatcaca ttagttaatg agcttaacga
     2161 acgaggtgta agtttccata gtttagaaga aaatataaca atggataaat caagttcaac
     2221 tggacaatta ttatttcatt tattcgcagc atttgcagaa tttgaacgta atttaatttt
     2281 agagcgttct tcagctggcc gaattgcagc acgtgctaga ggccgatatg gaggcagacc
     2341 agaaaaatta aatcaaaaag atttaaactt acttaagacc ctttatgata atggcacgcc
     2401 aattaagaca attgcagaac agtggcatgt ttcacgtaca acaatttatc gttacctcaa
     2461 taaattggag gacaaggaag atgaaaaaca aggagaagta tctaactaat ttttctgaag
     2521 ccaaacgtaa aaaagctact caaaagtaca atattataaa accttttatc ctaggcgaac
     2581 aatctttatc aagtatttct aaaagtaaag ggattgcgtt aagcaccctt tataggtgga
     2641 ataagtcata taaagaacaa ggacttaaag gactaatcta tgcgacaaga gctgataaag
     2701 gaacacgtaa aatagaacca aagataattg atgaaattga acgccttgcg cttatgaata
     2761 aaagaaatag tatagccaca attcatagga aaatcactaa ttactgtaaa gaaaataatt
     2821 ttgatatacc aagttataaa caaatttata gtgttattaa agcgatgcct aagtctgtta
     2881 ttgatttctc tcatcagggt gaaaaatact atcaaaataa gtacgattta atacaaatta
     2941 gagaatcctc gagacccaat gaaatatggc aagcagacca tactttgcta gatatcttta
     3001 tattagacca aaaaggtaat atcaatagac catggctaac tattattatg gatgactata
     3061 gtcgtgcaat tgcaggctat ttcttaagtt ttgatgcccc ccaatgctca aaacacagca
     3121 ttaacactac accaagcaat atggaataaa aatgatacca attggccagt gtgtggtata
     3181 cccgaaaaat tctacacaga tcatggaagt gactttactt cacatcatat ggaacaagtc
     3241 gctattgatt tgaaaattaa cttgatgttt tcgaaagttg gtgttcctcg tggtcgtgga
     3301 aaaatagaac gattctttca gacagttaat caaactttct tagaacaact gcctggatat
     3361 ataaacaata atgatactcc tagtaatcta ataaattttc acaattttga agaaaaatta
     3421 cgttactttt taattgaaga ttataatcaa aaagaacata gtactattca taccacgcct
     3481 atcagtcatt ggaatagtaa tcactttttc cctaatatgc caagtagctt agaacaactt
     3541 gatttattat tgttggaaat acctaaatct agaaaagttc attcagatgg cattcatttt
     3601 caaggttttc gatacagtaa tactaatcta gaagcttatg taggtgaata tgtactgatt
     3661 cgttataacc caaatgatat ggctgaaata cgtgtgtttt atagagatga atttctttgt
     3721 actgctatat gtccagaaat agctgattac tccatagata taaaagacat acagcacgct
     3781 cgtagtcaga gaagaaaaca cttaaaacaa aatattgcta gtccaagtac cacggaatta
     3841 attaaggaag aaaaaaaatg tggttattca cctcaagaaa cgactaaaaa cgtcaaaaaa
     3901 ttaaagagat atcgtaatga ctaaaaatca aaatttcatt gaaacgaaag agtataaacg
     3961 ctttgctgaa ttttgtgatg cttgtattaa atatcagtat attggtatat gttatggtca
     4021 acctggtgtc ggaaaaactt tatcgtctag gtattacaca aattgggata ctattgaaaa
     4081 acaagttaac catagaggtt gggaagattt agctagtaaa acgaccgatg acatactatc
     4141 ggtggataaa atattttata atgcaccagc agaaaaacaa actaaattaa ggaatgattt
     4201 atatagtatt actgcaatca ttgatttggg tcaaaaatta catgttgtaa ataagtatgg
     4261 acatgaacat agtaaacatt atagtgatat gtttaaatat attgatttaa ttatagtaga
     4321 tgagattgat cgccttaaag tgcaacattt agagcaatta agagctattt atgatgaaca
     4381 taatttagca atgatattta ttggtatgcc gggtatagag aaaaaactat ctcgttatcc
     4441 tcaactatat tcgcgtatag gttttgcgca tgaatttgat aatctaagta aagatgagac
     4501 gcatcatata ttagaatata agtggcaaga cttaggattt gatttaaaac tcgaagactt
     4561 tactgattat gaggcaataa cgacgattat taaaattaca aaagggaatt ttaggctgat
     4621 tcatcgttta tttgcgcaga tagacagaat tatggatata aatggtttag ataaaataag
     4681 tacagaagtt gtagaaacag ctagagatag tttagttata ggtattcgat aatagaaaaa
     4741 gcaacgcatt ttaatatagt gcgttgcttt tctcatttat taggtaaagt cattctcata
     4801 tatcgagtaa agtgacaaca attatatatg tatgattagg gtgtacttat atcattcata
     4861 taaatgttta tttataccct caataaaaat attatatgct tttaagcacc tatcacattc
     4921 cttataaaaa tgtttattca atcctttaat atcgactagc gtagtcttat aatttttttg
     4981 taacaatttt aaatcctctt cgtaaaagct taatttaaaa tgaccggctt gagctaatgg
     5041 cttttttcct tgtttaatta aaatcaatat gttttttaaa tgttttaaaa agtttttata
     5101 atcataaact tttattttca tgaatgattt atgtggatta ttttcataat ttagcaggta
     5161 gttttctata tcttcaatat aactatcatc aaatggtgtg tgctggaaat aaaaagtttt
     5221 tacatacatg aatattttat aaagttcttc tttcaaaaaa tcatatgtat ctaaatctct
     5281 ttgtatagca tccaattttt ttaatttttt tacattttta ccacaatcct gtactcttat
     5341 ccatccacta ttttctttaa tttcactagg tacactttgc attaaagtct gagatacttt
     5401 actttttaat tccgaaatat cattaatatt tggactaatt agtctatttt ctttaacttt
     5461 tgtcttaaac tcatctaatt ttttagactt ttccgcggat tcggtagatg ctttattcat
     5521 ctcaaaaaca tatataggtt tacctaaaga tttagcaaaa ttgtactcca tttctgtata
     5581 gctaatattt tcttcactat caattgttcc ataactagaa cctaacaata atatcactgc
     5641 atctgattca ttgatccatt gtgttattat ttcccattgg cttttactac ttgagttaaa
     5701 aagttccata cctgctggaa tattattact gtttaaaact gcttctacta ctgcttgacg
     5761 ctcttctatt aaatctaaat aagttgatga aataaaaatt tgatatttaa ttttagtatt
     5821 actcatatat gctcctttaa taaaaaatca atttacataa atatatcata tgataatatc
     5881 caaaaagact acttaaaata ttatcttttt gcattcctct actaaccagt gttataatct
     5941 agataacgaa aatgattaac aattaatgca ccaatcccca cactactgca atagtgtggg
     6001 gattttttcg atgctggctt ataaagtcgc ctattaatta tttgttgcgt ttactaagcc
     6061 aataagtata atacgcaaca gcacagccac tgatgactgg tgctatgatg tgaacgaaaa
     6121 tgattagcaa agtaatgcac ctcctctctg cgtctgaatt gacgcctgag agataggcga
     6181 cctgtatatt atatcatctc cgaatatacg atgatagata acttccgata agtcgtctta
     6241 tgttatataa ctttgttttt tatagtatca agacaagaag aaactcgttt cactcgtttc
     6301 aaaaaccctc ctgattttta gagaaaaact ctcttatttc atcgtcttta ataaaataat
     6361 atgccatctt tccatcttta taaaaatcaa gtacctcatt tttatataaa agccttaaat
     6421 gatgtgaagt tgaagcaaca ctcattttca atatcaaaga aatatcacaa acacacaact
     6481 catcttcttt aatcaaagat aatatgattt tcaatttctt ctcatcacaa attttttcta
     6541 aaatgttaag taatttctta gatttatcat cttctaaaaa acttaaagca ttgttaactt
     6601 tatcctcatg tacacagatc acatcacaag tattttcata actcatataa aatcacaccc
     6661 taaattaaaa tataaatcct aaaattgttt gaatagtgtc attttcaata ataataaata
     6721 aacctaaagc tatataaatg atagccataa tccaacgact aaatttctca acaatttctc
     6781 caattcctgg gatattagct aatttttgtg cagtaaacac caagaagaaa attaaaatta
     6841 aaaagacaaa cagagtaagt aataaattag taacacttaa tgtcacaaaa tacggaacaa
     6901 ataaaccaat attatcggcg ccacaacttg ctatcgtaac aattgcaacc gtaccaacta
     6961 atttagacaa tcctttttca ttcaattctt ttttagctct cttttctcct tcacaatcat
     7021 cataaatagc cactttaatt ccaagataaa ttggtattaa acccaataaa cctaatatcc
     7081 acttttctgg aacataattt aatacaaaag ctaaaaacaa actaactaat attaaaataa
     7141 tagaccctaa atattgacca acataaatat ttctatattc ttttctagtc tttgctctag
     7201 caaaaaatat taataaaatt actaataaat caactgctgt agcaatataa agaacagcag
     7261 cagcaacaac cgtttgaatc ataatacacc tcattcaaaa atatttttga atgtattata
     7321 acttatattt ttaaataatc ataggtgctt tattccaatt gctttattga cgttgagcct
     7381 cggaaccctt aacaatccca aaacttgtcg aatggtcggc ttaatagctc acgctatgcc
     7441 gacattcgtc tgcaagttta gttaagggtt cttctcaacg cacaataaat tttctcggca
     7501 taaatgcgtg tctatgcgta gacatggaga cacttcagaa ttatggagtg attacatgag
     7561 tttaactatt tttctttatt cagttttagg aagtttatta gcaaattata tctacgaaaa
     7621 atcttcaaaa taaataactt tttcggttct actttgtaca ctttatctac ctggtagacc
     7681 taattgttat tgctttacag gtctaaaatt cgtcgttata atctagttaa atcccgtaag
     7741 ggcgcactta tacgtcggat aaatcgacat gtgcaaaaac gtaatcgacc ttcgtcgatt
     7801 tttttatttc aaaattaaag aaacggcttg agatattccg agcagttata aaaattaatt
     7861 tgcgttgaat attgctacta cattcttgaa aaagtgatgt agatttatag cccataactt
     7921 ttttgaatta catcgacgat acggcgtcga aagaaaaagc tacctcactt tatctggggg
     7981 ggcttttatt ttttatgccc ttattttgaa ttttaagagg gcattttaaa gatttagggg
     8041 tgattcatat agttttatat ctaaaactta tatacgcttt taaaatgcaa atatgagcca
     8101 aataaatata tgctattttc aaaaacaaaa atttgagcaa aaacaataac tattttttag
     8161 acactgccca tttacatgca aattaaaaat tagcataaaa ttctttaaaa cttccccacg
     8221 ttcttctaaa ttcctgcagg gcttttctta tcaaaaactt agaaatttta atttaactct
     8281 aaaatctccg taagagcata agggaaattt gtccccatca atgaatgggg gatctggcaa
     8341 agccagatat ttcaaggctt cgaaaatgtt tttgtgtggg gtcataaaaa gctcaagtgt
     8401 cacacttttg tgacactaca tacagcattt gtcacaaaaa taagacagca ctttttttac
     8461 aaaaaagtaa taaaaaaaga catcagttat cactaatgcc ttaaataata attttgcttt
     8521 tcaaaattgc cgtaatcgaa tgtgcctttt cttcttcatt tgctatttta tcttcatcac
     8581 ttgtattaat taaattgcca tcttcagcat cgtctaagtt taaaatttta tgtttttatt
     8641 taagcaatcc accataactt aacatacgtt ttcgatacaa atctttttct agatcactga
     8701 caatttcttg attttttcat cgctatcagt taaaaagtca gatgatttaa cgaaatattt
     8761 tgatgtatct ttgattgtta taaaatcatt tgaatgattg attaaagata ttatattgtt
     8821 tcatgttttt acggcgcata taaagtgcat gtgtttcttc aaattcagtt aaaattgttt
     8881 gcattaaata accttgcatt gacttgaatg attactaatt tcattttcgg actctatatc
     8941 attcaagtca ttcatatcat ttatttcagt attgttagat tcagtatagc taagttcagt
     9001 atcattagat tctgttttat cgacttcttg aagtcggttt ttccgacttc cttgccattg
     9061 ctgctctcaa cagtgttttc ttgcttttga attttataaa tatcatcttc tgttacttct
     9121 ggtttaacta aataaagttt atttgtctta tttaaacctt gtctttcttg tagtagtaat
     9181 tcggcttcaa ttaagttctt tctttatttt aattacttta tcttttttac aatttaatat
     9241 attttgtggg ttttcatttt tgtaataaaa ataaatatta cctgttttag aatcaaccca
     9301 accattttta attgataaat ctaaacgatc tctaagaata ccccatgcca tttttgcatc
     9361 attggataac ttgatatatt taggatttgt aaaaaatact ttcggtattt gataaaactt
     9421 ttctctgtat tcctcattta tgttgtaaag tttaattgac ataataaaaa cctctttccg
     9481 aattttagtt tggtaagagg taataaaacg cttacatttt ttgtgtttat ttaataatct
     9541 aggtgcttgc actaagatat tattcattat ataatctaag tatcgaaaat tatataaata
     9601 aacagtttct aaacgtttta ttaccaaacc ccgactatta ccagtagttg gggtttttgt
     9661 tatattaagg ttattatagc actttgaata aacttaaaaa agggaaaata taggtttata
     9721 aacctttgat aaacaccata aaccctttac aaacccttga taaaaaggtt ggcgaattta
     9781 tatttttatt tgtcgattac tttattatta atcgttataa gcttataata ttttactaag
     9841 ggtttataaa cattttataa agggttgatg atatgaaaag tgtaaaaagg ttgagtgaag
     9901 aattaggggt tagtaagcaa acgattttta ataatattaa gagattgaat atagagacaa
     9961 taaaacagga aaacacttcg tttattaaag aagataccga tatagaaaaa atcattcaaa
    10021 gagtaaatga aaataaaaag aaatatggtt ttgaaagtgc tactgaagac aaacaaaaaa
    10081 aagagtcgga taatattaat tttgaatcta aaagcgatgc tcaaatagta gaaatactta
    10141 aaaatcagat taatacttta aataatcaaa ttgaaaaaca agaaagtcgt catgaaacaa
    10201 caattgaatt ttacagaaaa gagttgcaag aaagatcaaa attgcttgag aatcagcaag
    10261 ttctggcatt agagagtaat aaaaagattc aaaaattaga aaatcaatta gaaaaagaaa
    10321 aacaccttaa ttatcccttt gatacatcta ctaacgctag acaaaacgtt gatgcacaag
    10381 aaaaaacata tactactagt cctgtaaata ttaaccgaga tcaggaagaa actaaagaaa
    10441 cagagattca atataaggat atatctggaa gtcaaagcga cgaaagtaca caaggtgaag
    10501 aagcacaaag agaagacgtt tcagctaatc caaatgacaa tgatagtgat atagaagaaa
    10561 agagtgaaga aacggaacct aaaaaggggt tttggagtcg tctatttggt aactaatttt
    10621 aattaaaaag gtacgacaag ttcataaatt agccgtgctt ttttatgtga attgaacttc
    10681 tgataatcat cgattatgtt aaccaaacaa aaagccataa atgcctatat gacggcattt
    10741 atggcttttt actttcatta tcttttatgc atcttttata catcgttgat ttgacgcgtt
    10801 ttcattcttt tgcattggca agtattttgt atctttattc agttactatt tcttttctcc
    10861 aacctaacaa accatcttta actctaataa tatgtttgcc acgccagaca cttcttactt
    10921 cgaaataata ccattccccg tttttgttga ggaaataaat atcttgataa ccttctttaa
    10981 tgacatattt tgtagcttct tgtctttgat cgccaaacat tatcaatccc acccttatat
    11041 ttatttatct ttaatatata acaacattta ttaatattca tgttatttca tggaaggaga
    11101 gatttgttat aatgtgaagg tcgtctatct ctcagatgtc aattcggcgt agagaggagg
    11161 tgcgatttat gaatgaaatt cttgttcaca tcatgacaac ggcaatcagt ggttgtctcg
    11221 ttacgttatt tggttattag ctccataaac gtgataaaaa ataatataga cgattaaagc
    11281 caattcacca aaaaaccccg aactacggcg aatagttcgg ggttttgtgc gtttacaaac
    11341 gaaattcttg ttacttttag tatatatttc atattgcctt ttgtaaactc ttctattctt
    11401 atcaatacat ttcaaggtgt cgtaaaaata ctttcctttt tcatattgcc tgatgtgcat
    11461 taaattttga ttttaaaatc aacccatgaa cgaatgaatg gcccaaacta aaaatccggc
    11521 aacaggaaag aaaagccacc aaaaatcata aagaaaatca actatggaag gtaccctgtt
    11581 actctctcta taatttccct ctaaaataac ttttttaaaa cgtgcatctc ctttaattaa
    11641 atgctctaaa gagattccga ataaatcgct catagcaatc aaaatatcta aggaaggata
    11701 gtttttggca ctttcccatt tagaaacact ttgtctggaa acctttaata tattggctaa
    11761 ttcctcttga gaccatcctt ttaattctct ctgttctttt atagcttcag ctaatttcat
    11821 aaaatcactc cttaactcaa actataatta aaataattga aaagtcacga tacttttagt
    11881 taaccaactc gcaacgaaaa gttgcgttat tcccaattca gttcaagttg ttttaaaaaa
    11941 tcctctcgtt ctttctctaa ttgtgggtcg taatcttttt gaggttgctt ttcatatgac
    12001 cgttcttcta accatttagg cgtcttttca cgcctcacat attgtcttga tttatctatc
    12061 ttggacattt gaggttgtcg tttgttatga atgcgtttct cagcgtcaaa tgtgaactct
    12121 atccattcaa ttttacgccc ttttttcgct ttgattttat tgatagtaag attattaaat
    12181 atagaaccta attcaataat aataggttta aaaacatttt tgttaatatc agtcatacga
    12241 taagatttag gtatatctaa acgattttta aaatcatcga tgtgaatttt taaatagcct
    12301 gtatgtttgt attgtttaag taatctaaac atatttttag aatatgtaga tttaagccta
    12361 gtcatttctt ttaattcaaa ttttgtaaaa ttattagtaa ttgaattcaa tatgtgtttt
    12421 aaatttgaag aagttgatat ttctagatat tgttctcgct tatggattct ataatgattg
    12481 aataaaacga atctttctat tacatcatca tcttcatatc taatggttaa attcaacatc
    12541 tttttataca cattatctaa atcattagca aatctattaa tattacgtgt ttctggacta
    12601 taattactta atttttttaa ttcatcaaaa gacaaacgta atgtattagt atcttgctct
    12661 tttaatttat tacacatcgc aaaaaataaa ttaatttcag tagctgtaaa tcgccttaat
    12721 ggaactaaat tcatttcatt tttatataca actgtctctc ctgtcatgat acagccccct
    12781 ttattacgaa cctaaaggag tatcgtttga atatacactc ccatttgaaa acttttattc
    12841 acaataaact actttattat caatgtcaaa ccataaaagg agtacaactt tttaaaacac
    12901 tccttttatg tggcaaaaac actcctttta tgtggcaaaa acactccttt tatgtggcaa
    12961 aaacactcct tttatgtggc aaaaacactc ctttaggcat tgaaatccct tgttgctcta
    13021 gggctgaaca agcccctaaa aaggttttta aaaagattta taaaaatata ttaaaaagtt
    13081 cgaggggggc tacctatttt tcatcctaaa aaataattaa cctcaaacaa atgcatttaa
    13141 atatctattt ttataatgta taaccatctt ctctatcttc tttaaaaatg atttctcgtt
    13201 cttgtgcttg atatttcttc ttaaagaact gttcatcatt gttatctatc gtcattactt
    13261 cacgtatttt agaattgtct aaatgattat caatatgatt tattagtgta tgataacgct
    13321 cttctttcat catactttta atgattttaa aaacttgatt gaataactta agcgtataat
    13381 ttagttcttt atctttcttt tcattctctg acaacaaata attataacgt tgtgctaaat
    13441 cattaaattc ttcaaccagc ttttcatttt ccatatggac actagtcaat tcattaacta
    13501 aactttcatt ttgtttctct gcttttttta atgacttccc acttttaata cattcataat
    13561 catctaatat catctgtgct gctttaattt gtttagtgta tttttcaaaa tcagttttag
    13621 taattacaat attgcctgtt tcttgcttct ctttatgaaa ccatccacct acatttttag
    13681 ttttaatttc atacatgaca ctcagaagct ttttaagcgt atctaatgat ttttggtagt
    13741 tttgtatcaa tttatcattt tcttgcttta aataatcctc tatttgcttc tagcgcatat
    13801 gcatttgttt atgatattct gttttttgtt tataacggct aacttgatca tgtttttctt
    13861 ttgtaagttg tctagaaatt cctcgtttta aatcatagcc ttgcttatta atatacgtat
    13921 tgaatctatc ttggaattcg gttaatgctt ttttattacc tacaacttct ttcgcactta
    13981 aacgtccatc tttagtaata ggaactacgc cataatgcat atgtggtgtt atctcatcca
    14041 tatgtactgt cgcatataaa agattttctt taccgtattc ttcttccaag aacgctttag
    14101 catgcttaaa aaactatttt gtctcttctt cagataattg attaaaaaac acattatctg
    14161 atgtaattaa tccatcaata tgtttaatgg catctgttct aattttgcgt ttccctttat
    14221 aatttttttc gattttctca tcaatttttt tattaaaatc aaacttagta tcattgacta
    14281 aatcatagtt taaatatgac ttacttaaat caatatctat attttcataa tttttatttt
    14341 ctctttgaat atgtctttga atacctcttg tatttgtttt tgatttcact ttaacaactc
    14401 gaataattga ataagacata ataaatcaac ttccactgca tatgattata acacctaaac
    14461 acagaaaagt tttaacatac tagacttatt aaaaaccaaa taagtcatta aaaacgtgtg
    14521 ctctgcgagg cttgtttttt gtcatcataa aaaaggactg gaaaatccag tccttaaatt
    14581 aaaaaactca aaagtaatga ctaatcatat ttcatattgt tttttctcta tgtatggcta
    14641 tacctatcaa tatttcttaa cttctaagct cagaaatatt gactatgaca tcaaatcgtt
    14701 tcgcaagctc taaatcatgc gttacgacaa tcatagtttt attttcatca agtagcttca
    14761 ataataaatc aataactatt tttccatttt tcgtatctag tgacccagta ggttcatcag
    14821 ctaaaatgat tttcggatct tttaatatta atcttgccaa tgctactctt tgttgttcac
    14881 caccgcttag agaactaaca attctcttga tatctaaatc taggccgacc ttttttaata
    14941 cttctttctt taagttcata ttatcttttt tattcttttt gaactttagg gctaaatcta
    15001 aattttgact gattgtttga ttttcaagta aagcaaaatt ttgaaacaaa taccctaacg
    15061 tatatctata aaaatcgact ttctttttta gtaacttatc ttcaatatat atgcttcctt
    15121 gtgatacatc ttcgaaccca gcgattatgt taagtaaagt agattttcca gcgccacttt
    15181 ctccaactaa agcatatgat tttccttttt caaaattaaa gttacatccg tctaaaatgt
    15241 ttttattacc tttttttaca aaaacatttt caagttttat cactatcggt cacctcattt
    15301 taaaaagcta ggaatatctt ttgtaatgta ttctatcgaa cgcacatatt tccctttatg
    15361 atttactttc acatattctt ttgtaggatc ataacctaaa aaatccaatt tataactctt
    15421 tttttctcca tttttagagt atacagttat atttctatag tcctgggtat cttttggaac
    15481 tttggcataa gatacttttt cttttaacag tgggttaaaa cgatctaaat gctcatttct
    15541 aaccaataac agactaagta tgatgattaa tagtacaatt gttacaataa ttaaagttct
    15601 tttcattgtt taaatctccc tcattaaatc tttaaagtat ttttcaagat atttaatata
    15661 agcaatttga agtaaatgtt gaataatagc aatagacatt attatgaata gtaatatctt
    15721 tgaaccaaat aaaatatgcg tagtgtaagc taacataata gaaagtaaga tagaaattgt
    15781 taaataacgt acattactgc tgaataatga ataaccatga atttttctta ataatagttg
    15841 ctttttattc cagct
//
LOCUS       DAHHRR010000053        15657 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.053, whole genome shotgun sequence.
ACCESSION   DAHHRR010000053 DAHHRR010000000
VERSION     DAHHRR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15657
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.053"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            102..770
                     /gene="queC"
                     /locus_tag="NBI42_001467"
     CDS             102..770
                     /gene="queC"
                     /locus_tag="NBI42_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCD1777887.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEIAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            772..1191
                     /gene="queD"
                     /locus_tag="NBI42_001468"
     CDS             772..1191
                     /gene="queD"
                     /locus_tag="NBI42_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCD1777888.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            1195..1908
                     /gene="queE"
                     /locus_tag="NBI42_001469"
     CDS             1195..1908
                     /gene="queE"
                     /locus_tag="NBI42_001469"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCD1777889.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKSDIKLMTAEEIYDELKRIGGDLFNHVTISGGNAALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            2007..2591
                     /locus_tag="NBI42_001470"
     CDS             2007..2591
                     /locus_tag="NBI42_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777890.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNNLDETAENNNKIKKLMIKGLIIINI
                     SFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            2871..3311
                     /locus_tag="NBI42_001471"
     CDS             2871..3311
                     /locus_tag="NBI42_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HCD1777891.1"
                     /translation="MNTKYFLAVGAVASVLILGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            3653..4126
                     /locus_tag="NBI42_001472"
     CDS             3653..4126
                     /locus_tag="NBI42_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCD1777892.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            4101..4787
                     /locus_tag="NBI42_001473"
     CDS             4101..4787
                     /locus_tag="NBI42_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1777893.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            4787..5842
                     /gene="saeS"
                     /locus_tag="NBI42_001474"
     CDS             4787..5842
                     /gene="saeS"
                     /locus_tag="NBI42_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HCD1777894.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(5915..6898)
                     /gene="csbB"
                     /locus_tag="NBI42_001475"
     CDS             complement(5915..6898)
                     /gene="csbB"
                     /locus_tag="NBI42_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HCD1777895.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSYISFSRNFGKEAAMIAGYQHSTKFDAVIMIDCDLQH
                     PPEYIPKMIEGFMEGCDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVARQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSIENDEKDTHKVYSK"
     gene            complement(7030..7869)
                     /locus_tag="NBI42_001476"
     CDS             complement(7030..7869)
                     /locus_tag="NBI42_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1777896.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(8079..9428)
                     /locus_tag="NBI42_001477"
     CDS             complement(8079..9428)
                     /locus_tag="NBI42_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCD1777897.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVMLNYEFNEARPTIGQSDEDEKSE"
     gene            complement(9649..10830)
                     /gene="nagA"
                     /locus_tag="NBI42_001478"
     CDS             complement(9649..10830)
                     /gene="nagA"
                     /locus_tag="NBI42_001478"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCD1777898.1"
                     /translation="MSELIIYNGKVYTEDGKIDYGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVVPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLT
                     TTMTQSTDKIDKALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDEDMNVKSTIKQGKVHTF
                     S"
     gene            complement(11138..13102)
                     /locus_tag="NBI42_001479"
     CDS             complement(11138..13102)
                     /locus_tag="NBI42_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1777899.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKEGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDHADDEATKEEEAE
                     AEAQQVAAVEANQDTNGSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVET
                     NGSSGIKNHLTEQDIKNATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKA
                     LDTSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVF
                     LFGANSFDPKSSDYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGG
                     MLAISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLL
                     MIYVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGA
                     LIEGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAI
                     PFAAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALL
                     VGTLVSALIYGRIKPKLTETEIEASKSMDE"
     gene            complement(13108..14028)
                     /gene="pfkB"
                     /locus_tag="NBI42_001480"
     CDS             complement(13108..14028)
                     /gene="pfkB"
                     /locus_tag="NBI42_001480"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCD1777900.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDADVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIGKIKSQVTISVLDGE"
     gene            complement(14025..14789)
                     /locus_tag="NBI42_001481"
     CDS             complement(14025..14789)
                     /locus_tag="NBI42_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1777901.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSVMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(15038..15520)
                     /gene="ybaK"
                     /locus_tag="NBI42_001482"
     CDS             complement(15038..15520)
                     /gene="ybaK"
                     /locus_tag="NBI42_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCD1777902.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
BASE COUNT         5186 a         2840 c         2241 g         5390 t
ORIGIN      
        1 ttttatggag ataggctttt tttattttat aaattaaaaa cgggcttatc aatgccatta
       61 acctctttat taatattatt aatgatagga gcaattacaa aatggaaagt gtattaaata
      121 atgaaaaagc catcgttgtc tttagtggtg gtcaagacag tacaacatgt ctcttttatg
      181 caaaaaagca tttcaaagaa gttgaactcg taacgtttaa ttatggccaa agacatgata
      241 ctgaaattga aatcgcaaaa caaattgcac aagatcaagg aatgaaacat cacgttttag
      301 atatgtcatt attatcacaa cttactccaa acgcattaac acaacatgat atggaaatta
      361 ctaataatga agatggtata cctaatacat ttgttccagc tagaaattta cttttcttgt
      421 cgtttgcagg cgctctagct tatcaaattg gggctaagca tattattaca ggcgtatgtg
      481 aaacagactt ttcaggctac ccagactgtc gcgatagttt tataaaatca atgaacgtaa
      541 cattaagcct agctatggac aaagattttg ttattcatac tcctttaatg tggttaaaca
      601 aagcagaaac gtggaaatta agtgatgaac tcgaagtttt agattatatt cgtacaaaaa
      661 cattaacatg ctataacggt atcattgggg atggctgtgg tgaatgtcca gcttgtcatt
      721 tacgtcaacg tggactaaat caatatcttg aaagtaaagg agcgctttaa aatgttacaa
      781 caaatctatc ctagtactac gcatccatat caattcgaat taaataaaga ttttaatttt
      841 tcagctgcac atcacattcc gtgtgaagaa gcaggtattt gtcaaaatgt ccatggtcat
      901 acttactttg ttaatttaac aattgtcggt gataaactag atgacactgg ctttttagtg
      961 aactttagcc atttgaaaaa gatgatacac ggtaaatttg accatcaact gttaaataac
     1021 ttacctgctt ttaaaaacaa aatcccttca actgaaatcg tagcggaaac aatttatcaa
     1081 attgttaaag aaaatttggc atcgctcgaa caccaaccaa aatgtattca agtatttgta
     1141 agagaaacac caacaagtta tgtcgtattt agaccaaagg aacaggtgta actaatggct
     1201 aaaattcctg tactcgaaat attcggtcca accattcagg gtgaaggtcg agtaatcggt
     1261 agaaaaacga tgtttgtgag aacagctgga tgtgattatc gctgtagctg gtgtgattct
     1321 gcatttactt gggatggtag tgctaaaagc gatataaaac tcatgaccgc tgaagaaatt
     1381 tatgatgaat taaaacgaat tggtggcgat ttatttaacc acgttacaat ttctggtggt
     1441 aacgcagcat taattaaagg tattcaagag ttagttgact tatttcaaga taaaggtatt
     1501 ttcagcgcac tggagacaca aggtagtaaa ttccaacctt ggatgacaca aattgatgat
     1561 ttaacgatta gtccaaaacc accaagttca actatgacac ctgatttaaa aaagttagat
     1621 gaagtgatta cacaatgcgt accttcctca ttaaacttaa aagtagttgt attcgacgat
     1681 aaagattatg attttgccaa aatgatacac caccgttatc cagatattcc cttctattta
     1741 caagttggta acccatattt atcagacagc gtagataatc ataccgaaaa gttgttagaa
     1801 cgctacgagc agttggtgga tttagttatg caaagtaatg atatgaatca cgtttatgtg
     1861 ttaccacaac ttcatacatt actttggagt aataaaaaag gtgtataaag gataacttat
     1921 tttacttttt acaagtcata tctaatgaat aacaatatat taattgatat aatagttgat
     1981 tgaagtataa cgaacaggag tccgtcatga ttgtatatat attaattaat attgctatcg
     2041 tcgtacttat aactggattc aatttatata ggcatcaaat gcaacattta tcattaagtg
     2101 caatgttgtt atctattact ataaacgcct ttataaatac attcataatt gataaatata
     2161 actttattac tctgtgtact ataacgatgt ttatcatttg gacgatacta cagttttaca
     2221 ttgataaaaa attaaaacct gtatacatta cagaccaaaa atttattgct atcatattaa
     2281 cgattgttgt cagtttaacg caacgtgtta ccgacttttc aagtacacaa tcaatatata
     2341 tgtctattcc atttttagcc cctgccattt tcatcattgg tggtattatg ttgtttattt
     2401 caacatttaa taacttagat gaaactgctg aaaataataa caaaataaag aaactaatga
     2461 ttaaaggact tatcattatt aacatttcat ttatcgttat gatggtttta acaccatatt
     2521 ggtacttgta tttaatcgtc tatcttattt tcttgttgtt tttattgtgg caaaaggttt
     2581 ataaatttta ataccaaaac tataaaacac ttctgatatt cttagttcga tatatcagaa
     2641 gtgttttata gtgataacta gtttagacaa atattttctc acttaaaaaa ccgccctcct
     2701 cttattttga ctcctattta tttaaatcag acaattattt tcattttcaa attattcttt
     2761 cttcaatatt agttaagcga tatttaaacg aagttaagaa ttagttaatg gcatattatt
     2821 tgccttcatt ttaaacttaa cttatcaaat tgaagaaatg aggagttagc atgaatacaa
     2881 aatatttttt agcagttggt gctgttgcct ctgtattaat tttaggcgct tgtggtaatt
     2941 ctaattcaca agatcaaggt aacaaaactg aacaaaaaac aaagtcagaa gatagcaatg
     3001 ttaaaactga taaaacaaaa cacctaacag gtacattcag ttctaaaaac ggtgaaactg
     3061 ttgaaggtaa agctgagatt aaaaatggta aattaatgct tactaactac aaatcatcaa
     3121 aaggtccaga tttatacgtc tacctaacaa aaaatggtga cattaaaaac ggtaaagaaa
     3181 tcgcaatggt tgactacgat aaagaaaaac aaacatttga tcttaaaaat gtagatttaa
     3241 gcaaatatga tgaagtaaca atctattgta aaaaagctca cgtcatcttc ggcggcgcta
     3301 aattaaaata atcaatttct gagttaaact tttatttaca acatactatt actatacatt
     3361 acaaatttta aaaatataca ttacactcat tactcaatgg aaagcgtatg atttcacagc
     3421 ccccctagct tgtagaaatc atactttcct tttttcaata tatatacaac tattaaatcc
     3481 cataagattg tagagcacat aagtaaattt ttttagagct tgaggtttgt ttagcttaag
     3541 caacccatga gctcaaacac ttcctgttca cataacacta taaatcacat tatgttgctt
     3601 aatcttatgt ttatataaat tacacacaat aaatagaaag aatgtgaaca tcatgaataa
     3661 attattgcta ctcgttacat ttatcattcg tgtgggttca ggtattgtta tgttaatgca
     3721 aggttacgaa aaattaacgg gcggatttac gctgaaaggt ttagtaccag tcatcgctaa
     3781 caatactgat tcaccagagt ggtataagtg gtttttcgca aatatagttg cacatacgac
     3841 gtcattattt gatattgttg tcccactcgg agagattgca attggattag gtttaatttt
     3901 tggagttttt gcatatgctg ctagtttctt tggagccttt gttatgataa attatatctt
     3961 agcagatatg atatttacgt atcctcttca attaactttc tttatccttt tactaatgag
     4021 tcactcattg ttaaaacaga tttcacttaa agaaatcatt aattacttta gaggtcgtaa
     4081 gaacagaggt gaaaaaatag atgacccact tactgatcgt ggatgatgaa caagacattg
     4141 tagacatttg tcaaacctat tttgaatatg aaggttacaa agtaacaacg acaactagcg
     4201 gtaaagaagc aatttcttta ctatcaaatg atattgatat catggtactt gatatcatga
     4261 tgccagaagt taatggttac gacattgtca aagaaatgaa aaggcaaaaa ttagatatcc
     4321 cctttatcta tttaactgcc aaaacacaag aacatgatac catttacgcc ttaactttag
     4381 gtgcagatga ctatgtcaaa aaaccattta gtccaaggga actcgtttta cgtattaata
     4441 atttacttac aagaatgaag aaataccatc atcaaccagt tgaacaactg tcgtttgatg
     4501 aattaacact tattaactta agtaaagttg tgactgtaaa tggtcacgaa gtccctatgc
     4561 gtattaagga atttgagtta ttgtggtatt tagcttctag agaaaatgaa gttatttcta
     4621 aatcagaatt acttgaaaaa gtttggggat atgactatta cgaagatgct aataccgtga
     4681 atgtccatat acaccgtatt agagaaaaat tagaaaaaga gagctttaca acatatacca
     4741 tcacaactgt atggggatta ggatataaat ttgaaaggag ccgataatgg tgttatcaat
     4801 tagaagtcaa atcattattg gcgtcgtatc gagtatacta ttaacttcaa ctattttagc
     4861 aattgcatat attttaatgt ggtttaacgg ccatatgaca ctaactttga ccttaacgac
     4921 aataattaca agctgtttaa ccttattaat atgtagtatt tttattaatc cacttataca
     4981 aaaaattaag cagtttaata taaaaactaa gcaatttgct aacggaaatt acgcaagcaa
     5041 tgataaaacg tttaattcac caaaagaaat ttatgaatta aatcaatctt ttaataaaat
     5101 ggcttctgaa attacgcaac aaatgaatca aattaaatcc gaacaacaag aaaaaacaga
     5161 actgattcaa aacttagccc atgatttaaa aacaccttta gcaagcatta tttcatattc
     5221 tgaaggacta cgtgatggta taatcactaa ggatcatgag attaaagagt catacgacat
     5281 attaattaaa caagcaaaca gattatcaac attatttgat gatatgactc atattatcac
     5341 tttaaataca ggtaaaacat atcccccaga attaatacaa ctagaccaat tacttgtatc
     5401 aatattgcaa ccatatgagc aacgtatcaa acatgaaaac cgcacattag aagtgaattt
     5461 ctgtagcgaa attgatgcat tttatcaata tcgaacgcca cttgagcgta ttttaacaaa
     5521 cttacttgat aatgcgctaa aattttcaaa tgttggtagt cgcattgata ttaatattag
     5581 tgaaaataaa gatcaagata ctatcgacat tgctattagc gatgaaggta ttggcattat
     5641 accagaacta caagaacgta tattcgaacg aacattcaga gtagaaaact ctcgtaatac
     5701 aaaaacgggt ggttctggat taggcttata tatagctaat gaacttgcgc aacaaaataa
     5761 cgcaaaaatc agtgtaagca gtgatataga tgtaggaact acgatgactg taacattaca
     5821 caaattagac attacgtcat aatccgattt atttataaaa taaaatgcaa agactaaaaa
     5881 gaagctctca ttgatgaggg cttcttttat ttgtttattt agagtaaact ttatgggtat
     5941 ccttctcatc attttcaata cttgaaagat ttgtagcttg aataatatat ttaggacgtg
     6001 ccttaacttc ataatatatc ctgccaatat attcacctac aacaccaatt gaaattaact
     6061 gtatgccgcc taataataaa atagctgcaa tcgttgaaaa atatcctgga atattaacac
     6121 cagaaatcat aatattgatg aataaatata taatatataa cacgcttatt gaaaaaataa
     6181 ataatccaag atatatcatc attctcaaag gtttactatt aaaggaaatt aatccatcaa
     6241 taccataatt aaataacttt ttaaaggacc acttagattc tcctttttgt ctcgcaacat
     6301 tttgatacgt aaacacttta gtattatatc ctatccattc aaataaccct tttgaaaatc
     6361 gattatattc ttcaagtgat gcaatggatt taacagctct ttggcttaaa agtctaaaat
     6421 caccaacacc atcatcaaat tgtacctctt ctacaaagca attaactaac ttatagtaca
     6481 aatggcttaa tgttttgcga ctaaaatttt caccacttct atcacgcttt gcaatcactt
     6541 gatcacagcc ttccataaaa ccttcaatca ttttcggaat atattcaggt ggatgttgca
     6601 aatcacaatc tatcatgatg actgcatcaa atttagtact atgctggtaa cctgcaatca
     6661 tggctgcttc ttttccaaaa tttctactaa aagaaatata gctgacatgc ctatctattg
     6721 tggcaagatg ttgcatttca tctatagtgg tatccgtact accatcatct atgaaaagca
     6781 tattataatc atagcctttc acactactat cttgtgaaag tatttcagtt aattgttgat
     6841 gtgtttgtgt aatgacttcc ccttcattaa aacaaggaat gacgactcta attttcatct
     6901 atgtccatcc tttttattta aaagattttg aatgtacctg agtgttgttt tatcaaatag
     6961 taaaaaccaa ctcatttatt ttaatgtaca cattaaaagg cattcataaa cgtaaaattt
     7021 aacatccagt tattttaaat ccccaatttt cacatcatct ggatttttac cttgtcttgc
     7081 atttctattt aaagcatcaa tttcagctac ttctgttaat tctaaattaa aatctaaaat
     7141 atctatattt tcttgaatgc gtttaggtgt ctgagatttt ggaataataa ttctattgtg
     7201 tgctaaatgc caacgcaata caacttgtgc tggtgtttta tgatattttt cagcaatttt
     7261 aacaatgaca gggtcgtcta gtagtcctct atttctcatc aaaggcatcc atgcagtcac
     7321 tttaatatcg tgacgatcac aaaattcttg taattcttgt tggttaaaat atggatgtac
     7381 ctcaatttga ttcaccattg gtttgatact tgattgagcc attaattttt ctagatgatg
     7441 aacattaaaa ttacatacac ctattgcttt taccttacct tgctcgtaaa gttcttccat
     7501 agctttatac gtttctaaaa atagaccatc tgcttcacaa ggccaatgta ttagaaataa
     7561 atcgagataa tcagtttgta aattttcaat cgatttgttg aaatattcga atgttttctc
     7621 ataaccttga tagtcattcc ataacttcgt tgttataaac aaatcttctc tatcgacgcc
     7681 attatccttt aatgctcgcc ctagtgaagc ctcattatca taaaagtatg ctgtatcaaa
     7741 cgctctatag cctgcgtcaa ttgcagcatt tacaacttta gtcatatctt cgtcagagat
     7801 tttataaaca cctaacccaa ctgaaggcat cgggtatcca ttatttaata tttgtatctc
     7861 attcaacatc tttatatctc tccaatctat ttatctttta tatctttaca ttaccctaaa
     7921 tttttcaaca aactcaatta atacgaatta tcgctttcaa taaaaatcat taaagatatt
     7981 gagttccaat actattttca cttttcatta attttagtca aaaaaataac caaccaaaaa
     8041 tgaattaaat cattcttagt tggtttatat attaatatct attctgattt ttcatcttca
     8101 tcagactgtc cgatagtagg tctcgcttca ttaaattcat agtttaacat cacccaaata
     8161 atttggtggt tatcgatttc tgaaacaacc cagcgatcat aagttgtatc cacgtaatca
     8221 tctttttgta agttggtatt acgagattgt aaccatccac ctatcgtatc aatatcctca
     8281 gagtcatcaa attctatacc gaactcttca gttaaatcat ccaatagtac tctaccattt
     8341 acttggaatg tcttattatc aattttaacg atatcattca cttcatcatc atcaaattca
     8401 tcacgaattt ccccaacaat ttcttctaaa atatcttcca tcgttaaaat acctgccgtt
     8461 ccaccatatt catctataat aagactcata tgaacatgtt cacgttgcat tctaattaat
     8521 gcatcactta tacgtgttgt ctctgaaatc attggcaact catgtatata gtttgctatt
     8581 ttaatcgttt ttccagaagc atattcagtt aaaaattctt tgacgttaat aaatccttta
     8641 atgtggtctt tatcaccatc atcagtaatt ggataacgcg taaattgatg ttcttttatt
     8701 gtttctagta attcgtctac attaaaaggt tcatttagtg taatcatttg agttctaggt
     8761 accattatat cttttgcatg tctttcatcg aatgaaaaga tattttgcat atacgccaat
     8821 tcagtttggt tgatttctcc accattataa ctattgttaa taataatttt gatttcttct
     8881 tctgacattg catcggtttg ggcatcagga tttacaccaa acattctaat aataacacgt
     8941 gcagaaccat tcatcagcca aatcaatggt ttcataatgt taccgaaata gaacaatggt
     9001 cttgcatata ctaaagcaag cttttcagta tgttgaatcg ctatagattt aggcgctaat
     9061 tcaccaagta ctacatgcaa atacgtaacg attataaatg atactgcaaa cgaaatcgtc
     9121 gtcgttaatg cagttggtaa attgattgct tcaaatatcg ggtgtaatag cttttcaaac
     9181 gttggttcac caagccaacc taaccctaaa gatgttactg ttatacctaa ctgacaagca
     9241 gaaagataat aatctagatt agcaatcatc ttttttacta ttttagcagg tttatttcct
     9301 tcatctgcta gctgttcaat tcttgttgct ctaattttta ctaatgcaaa ttctgaacca
     9361 acaaatacag tggttaatgc aattaataga ataaatataa tcaaactaat tatggtcgaa
     9421 gtttccaatt aattccctat ttctagggat tcacctccat gttatatgtg tcactcatgg
     9481 gtaacacgca ttcaaattta tcactatgac ttaaattaca actacatgtt atcgccttcc
     9541 catttagaca cccccagaaa aaaatgttat tgcttctatt ttatcataat ataaagtgct
     9601 tatgttaaca gattaaatct attgcataca tttaattatg attatctatt aactaaatgt
     9661 gtgaacctta ccttgtttta ttgtagattt tacattcatg tcctcatcta gaataacaag
     9721 atctgcatcc ttatttactt taatactacc ttttctatca tcgataccta atgcaatggc
     9781 ttgatttaaa cttgttacac gccataaatg atctaatgta tcacctgtaa atgatattaa
     9841 gttacgtaac ccatgattca tttttaaaat actaccagca agcgcaccat ttgcaagacg
     9901 tgcttgttgc gattgaacag ttactttttg accacccaaa tcatattctc cttcaggcat
     9961 accttttgca cgcattgcat cggtaattaa ataaaaacgt tcattacctt tcatacggta
    10021 agcaattgca accgatgccg gatgagaatg tgtgccatca acaatcattt cggtatgtaa
    10081 agcatcattc aaccatgctg ctccaaaaac acctggttct ctatgttgaa atggcgtcgc
    10141 tgcattatat aaatgcgtga catgtttagc tcctcgctca acagcttcaa ctgcttcttc
    10201 gtatgttgcc actgtatgac caattgaaaa aataatgtca tctttatacg tttcaagcgc
    10261 ttcttttgca ccttcaactt caggtgcaaa cgtcataatc tttattaatc cgttagcagt
    10321 ctcttgaaaa tgtttaattt tatcgataaa tgggcgtaca acgtattgcg gatgttgagc
    10381 accaacttta ttttcagata taaatggtcc ttctaaatgt atacctacaa tttccgctgc
    10441 attgtgaaca tcttgctccg cttcatattt agcaatattt gtaagtgctt tatctatttt
    10501 atcagtagat tgcgtcattg tagtggtcaa gtatgatgtc gtcccttcag acaacaaatt
    10561 ttcggataga tattttaagc catcgtatga cccatccatt gcatcctgtc cataaccacc
    10621 atgaatatgt atatcgataa aacctggcac tacatgatga ccttttgcat caatcacttg
    10681 aattttattt gtcgtatcat tatcaattgc tgctttatca tccacttctc caattgcaac
    10741 aatctgtcca tctttcacat gaatgtaacc ataatcgatt ttgccatctt cagtataaac
    10801 tttgccgtta tatataatta attctgacac gtataaaccc ttctttattt ataaattttc
    10861 actttaagac actcatattt tactatatct gcttcgtttg gcataacttt ttgcaaaata
    10921 ttaaaaaagt ctatacattt gtggtgccta tttcttcatt catttatcta aaaataaaaa
    10981 caccttcata tcaattcata aaaactgaat taatagaaag tgtttttaca gctttgtata
    11041 agctaccgtt aacacgatat ataaaactaa catatattca ttgaacttac aacttaatat
    11101 gaagctcttt gctaacaatc actttaaatc attaaaacta ctcgtccatt gattttgaag
    11161 cttcgatttc tgtttcagtt aactttggtt taatacgtcc ataaattaat gctgaaacta
    11221 atgtaccaac tagaagtgca ataagagttt gaagtaagtg tgcaccatca gtaccaacaa
    11281 ttacaataat accaccgtgt ggcgcagtaa ttcgagaacc taagcctaaa gcaattgcgc
    11341 cacctatacc tgaaccaatc atcattgaag gaataacacg taatggatcg gcagctgcaa
    11401 atggaatcgc accttctgta ataaacgaca tacccatcac atagttaggg ataattgaac
    11461 cacgttgttc ttttgtaaat ttacgtctga aaattaacat cgctgtcgca attgctaacg
    11521 gtggaatcat accaccaatc attgcagctg taattggtgc tgcattacct tcaatcaacg
    11581 cacctgttgc aaaaacatat gccgctttgt tgaatggacc acccatatca atcgccatca
    11641 tagcgccaat aactaaacct aataatacaa tattagaacc tgataaattg tttaatccat
    11701 ctaataacaa atgatttaac caagatgctg gtggattaaa gacataaatc atcaataagc
    11761 ctgtagctgt cactgttaat agtggataaa ttaatgtcgg ctttaatccc tctaatgctt
    11821 gtggtaactt acgtgtcatg gctttaacac cttgtgttaa gtaacctgct aagaaacctg
    11881 caataatacc accaataaat cctgaaccac ctgaaattgc taacatacca cctacaagac
    11941 ctgacgcgaa tccaggttta tctgcaatac tacgtgcaat gaagccagat aaaattggga
    12001 taattaatgc aaatgcactt ttactaccga tattccaaag ttgttctgca aacgcgttat
    12061 aatctgaact tttcggatca aatgagtttg caccaaataa aaacacgatt gccattaaaa
    12121 taccaccaga tattacaagt ggcaacatgt tggaaacacc gttcattaag tgtttataga
    12181 aagctttacc tgggcttaat ttttcttgac tgtcatttga gttacctttt ctttgaccat
    12241 cacgggcaac aaaaggttta cgacttgtat ctaatgcttt attaattaat tcttctgggc
    12301 gtttaatacc atctgctact ggtacttcta cgacattttt accatcgaag cgatccgtct
    12361 caacatgaac atcagcagca acaatgatac ctgttgcatt tttaatatct tgttccgtta
    12421 aatggttttt aataccactt gaaccgttcg tttcaacttt aattttaatg cccattttat
    12481 ccgcttgctt tttcaatgca tcacgcgcca tatatgtgtg tgcaatacct gttggacaag
    12541 cagttacagc taatacatat ggctcgtttg atccattagt gtcttgattt gcttctacag
    12601 cagcaacttg ttgtgcttca gcttctgcct cttcttcttt tgtcgcttca tcatcagcat
    12661 gatcgataat tgctaacact tcttctggag acgatgcatg taataatttt tcacgtacat
    12721 tttcatccat taaaatacca gacaacttag ctaaagcatc taaatgtgtt tgggcaccac
    12781 cttctggcgc tgcaatcata aagaataaat gagctggttg catatctaaa ctttggtaat
    12841 cgacaccttc tttagattta ccgaatgcga tagctggaga cttaactgcg gctactttcg
    12901 catgtggaat agcaatacct tctccgatac cagttgtact ttgtgattct cgattgtgaa
    12961 tcgcttcctt aaatgacgct acatcactta atttacctgc tttgtctaat tgatttacta
    13021 actcatcaat aacaccattt ttgtcatttg ccattaaatc cattgctatt gtatcttttg
    13081 ttaataactc tgttactctc ataattttca ctccccatca agtacgctaa tcgtaacttg
    13141 tgattttatt tttcctatag cgtcccgtgt tgctaagtcc tcatcaaatg ccgtggcagt
    13201 accgcatgct actgcttgtt ggaatgcttt ttcaatcgtt aaacctgaag caattccagc
    13261 caccatgcct gcaactgtac tatcaccaga gccaactgta ttaaccactt tcccttgtgg
    13321 attaactgct ttaatactga tttctttatc aatataaata gcaccatcac cgccaagtga
    13381 gacaataaca gattgcgcac ctttatcaac tagcaaacga ccatatttaa taacatctgc
    13441 gtctgagttc actgttgtat taaacatcac ttctaattca tctttattag gtttaataaa
    13501 tagtggatga tatggtaaaa cgctttcagc caattctttt tcagcgtcga ctactaattt
    13561 agcacctgtc tgtgctgtaa tttgtgcaat ttgtgcatac gcatcgcttg gaatactact
    13621 tggtacactt ccagcaacaa taactatatc ttcgcttgtt gtatttttaa tttgttgtaa
    13681 cagttgttca aattgtgttg acgttatatg aggacccggt gcattgattt ctgtttcttg
    13741 tcctgttttt aatttcacat taatacgtgt atcttcatca acttcaataa aattcgattg
    13801 aattgcacta ttatttaatg tatctataat gaatttccca ggaaatccac ctgcaaatcc
    13861 caaagcagtt gattcaacat ccaatgtctt taaaacacgt gagacattaa taccttttcc
    13921 cccagcgaat ttatatgttg ctgttgctct gttcaaacca tcaattttaa aatcattcgt
    13981 aaaaatgaca tagtcaattg aaggattgaa agtcactgta taaatcataa agtccctcct
    14041 ataaagtgat acttttgttg gtattctttt aacgattctt gatttaatgc tttttcagat
    14101 gtgatgattg tcgtactttc tagcaaaggt acacgagcaa aatatacttt attaaactta
    14161 gaatgatcta taagtacaaa tgattgattg gctaatgaca ttgctgtttg tttaactaat
    14221 gcctcttgct catcgggagt agttaatcca agttcaatat ctaatccatt catcccgata
    14281 aaagctttat cgaaacaata tcgtcttaat atctccatga cacttgaacc aatcgtagca
    14341 agtgtatttt ctttaacttg accacctagc ataattgttt taataccttt tttaagtaaa
    14401 gcttctacat gtgttaaacc attggttacc acaatgatat ctttcgcttg aatgtattta
    14461 attagctcca atgtagatga accagcatcg ataaataagc attcattatc gttgatttga
    14521 ttagctgcta ttttagcaat cattttcttt tcatcaagat tagttgctaa tttttcagtt
    14581 aaattcgcct caaccatacg attttctttt aacattgcac caccatgcac acgttgcaat
    14641 ttccctaatt gttgtagttt agataaatct cttcgtattg ttgaagcact gcagccagtt
    14701 cgatctatta attcttgtaa agtcaaaaaa tctttgtgag aaagttcttc taatattaac
    14761 tcgtgtcttt tttctgtaat tatcatcatc accccatctc actttgtatt ataatgagta
    14821 cgctttcact tttcaatcat aaattaccaa aaacaatcac aaacaatcaa attgtgacga
    14881 taaaattaca tttttatgtt cgaatactca attatcatga ttgtttatga tcgtaaaatg
    14941 ctaattcaat caaaaagagt gtggcagaaa tgaattttat aaaaatttca tctctgtcac
    15001 actcctaatc ttgccccata ctttttgttg ttattaatca ttcatggata actgctccaa
    15061 ttttgccttt agttattgta atcaaatcct caacagctat cgtgatttgc attgttcgaa
    15121 gcccaccact cacactgata tgactataat tttcacagga tttgtcaacg actgttggaa
    15181 acaatgtttt cataccaaca ggtgaacacc caccacgaat gtatcccgtt acatttttta
    15241 aattatctaa aggcataagt tgcaatttct tctctccaac taaagcagct gcctttttca
    15301 tatctaaagt ttcacttact gggataacaa atacaaaatg ttcatgtttt gtattttcta
    15361 gaacaagtgt tttaaatact gttttaacat ttgcttgtat gagttctgcg acttcttggc
    15421 cagataaatg ttcttctggt acctcaaagc tatgaacttc gtatttaatt tttgcacgat
    15481 caagcatacg catcgcgttc gttttcttat ttttagccat ttcaacacct catttatact
    15541 tcttcagaac tatattaaca ttttaaaata cattacggat tggttgattt tgacataaca
    15601 tttacatcat tttataagtt gatttcagct ttttaaatta aattattgca aaataaa
//
LOCUS       DAHHRR010000054        15485 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.054, whole genome shotgun sequence.
ACCESSION   DAHHRR010000054 DAHHRR010000000
VERSION     DAHHRR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.054"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(246..1082)
                     /locus_tag="NBI42_001483"
     CDS             complement(246..1082)
                     /locus_tag="NBI42_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCD1777903.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFEDENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(1085..2071)
                     /gene="pta"
                     /locus_tag="NBI42_001484"
     CDS             complement(1085..2071)
                     /gene="pta"
                     /locus_tag="NBI42_001484"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCD1777904.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLNLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYTGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QHIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2239..2991
                     /locus_tag="NBI42_001485"
     CDS             2239..2991
                     /locus_tag="NBI42_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HCD1777905.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRLVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
     gene            3541..4305
                     /locus_tag="NBI42_001486"
     CDS             3541..4305
                     /locus_tag="NBI42_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCD1777906.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARVLLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKVYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            4318..4770
                     /locus_tag="NBI42_001487"
     CDS             4318..4770
                     /locus_tag="NBI42_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCD1777907.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(4911..6395)
                     /locus_tag="NBI42_001488"
     CDS             complement(4911..6395)
                     /locus_tag="NBI42_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCD1777908.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVSVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(6516..6884)
                     /locus_tag="NBI42_001489"
     CDS             complement(6516..6884)
                     /locus_tag="NBI42_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCD1777909.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            complement(7017..7397)
                     /locus_tag="NBI42_001490"
     CDS             complement(7017..7397)
                     /locus_tag="NBI42_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HCD1777910.1"
                     /translation="MVNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SKTPHIGEQQMNHRIANHNQMPQSQITQPTHQVTAAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            complement(7398..8054)
                     /locus_tag="NBI42_001491"
     CDS             complement(7398..8054)
                     /locus_tag="NBI42_001491"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCD1777911.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANTYLESVGKSPINWCESEA
                     "
     gene            8238..9068
                     /gene="thiD"
                     /locus_tag="NBI42_001492"
     CDS             8238..9068
                     /gene="thiD"
                     /locus_tag="NBI42_001492"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCD1777912.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            9602..9769
                     /locus_tag="NBI42_001493"
     CDS             9602..9769
                     /locus_tag="NBI42_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HCD1777913.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(9960..10238)
                     /locus_tag="NBI42_001494"
     CDS             complement(9960..10238)
                     /locus_tag="NBI42_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777914.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLEAN
                     QCQINYVKPILINENIEFIEEHEQEMIAINDDGEIKIKISLSTKNNRY"
     gene            complement(10241..10606)
                     /locus_tag="NBI42_001495"
     CDS             complement(10241..10606)
                     /locus_tag="NBI42_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HCD1777915.1"
                     /translation="MQHYSLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WSKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQIRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            complement(10581..11720)
                     /locus_tag="NBI42_001496"
     CDS             complement(10581..11720)
                     /locus_tag="NBI42_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCD1777916.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDISRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKEIMIASMGIGGGLGNAALFTRF"
     gene            complement(11722..13098)
                     /locus_tag="NBI42_001497"
     CDS             complement(11722..13098)
                     /locus_tag="NBI42_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCD1777917.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDEIITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     EDTGLMQSIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSYSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCRKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQHDMLIIGGQNIYPAHVERHLTQSSSIDEAIIIGIPNK
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSKIHHVEKMYYTASGKIAREKMI
                     SMYLRGEL"
     gene            complement(13643..15043)
                     /locus_tag="NBI42_001498"
     CDS             complement(13643..15043)
                     /locus_tag="NBI42_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1777918.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVESADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKD"
BASE COUNT         5033 a         2799 c         2353 g         5300 t
ORIGIN      
        1 aacatttcaa tagaaggttc gtttctttca tagtcatacg cacaatatat accctattct
       61 caatctattt aatacgtaaa acatgaaatt ttcttattaa atttattatt tcaatcatat
      121 cattactttt aatttaatga tgttcaattt aaatattagg tcaataacat atttatgctt
      181 tttatggata ctttcaaaaa taacagcccc aaacgataac ttgaaagggg ctgttaaata
      241 tttaactatt gcatttggtc tatcattttc ttgtttcttt caatcatttt atcaaaatac
      301 gtatcgtatc tttgccattc ttcttgagta attggcgtca tatttaatac accgccaaga
      361 tctttgattg catataatag taaaaacatt acatcttgaa cagtaatcgt tttgttaagc
      421 aatgtctcta gtgaggccat acaagatggt tcaatttcag ggtatttaaa tttagtcact
      481 tcacctttta aagcgtgttc ataaaatgtt tgcatcatca atgcacgttc tgaaccagaa
      541 ccttcaacac aaagataaat ttgtacagca ataccgcctc taactcttcg ttgcgatatg
      601 cctgcaaatt tcttaccatc gatacttaag tcaaattttc ctgggcaata agaatgttca
      661 atttccatcg tatcaatatc aacattctca tcttcgaaca ttttgctaat caagagatac
      721 atcacagtaa acgcttcatc aatcgttgtt tctgtttgtc ctttgaacat cagcgatata
      781 tttaatacac cttgatctag aacgacacct aagccaccag aatttctaac aatggcatta
      841 taaccaatct cattcgttaa ataatcaatg ccatctttta aaaacggcaa tcttgaatca
      901 tgaataccaa gaataacagt atgttgatga atccaagtac gcacaacatt atctgatata
      961 tctttgccca cactttcgca aaatgtatca tcgaatgcga aagattgcat aggctctaat
     1021 ccagaagaat ggtcgatata tcgccagttg acgccattaa aatatttact cgctaaatcc
     1081 atcgttattg taaggcttgc gctgctgtga taattgaaag attgtataca tcttcaattg
     1141 agcagccacg tgataagtca tttactggag aatttaaacc ttgtaatact ggaccaactg
     1201 catcatatcc acctaaacgt tgtgcaattt tgtaaccaat attaccagct tctaaacttg
     1261 ggaatacaaa gacatttgca tcaccttgta atttagcacc tggcgctttt ttctcagcaa
     1321 cacctggtac aatcgcagca tcaaattgga attcgccatc aatgattgct tctaattttt
     1381 cttcttcagc tttttgttgt gctaatttga cagcttcttg aacttttgtc acgtcgtctg
     1441 atttagcaga cccttttgtt gaaaagctta acattgcaac ttttggatcc atgccaaagc
     1501 ttaatgctga ttttgcactt tctactgcaa tttctgcaag tccttgtgaa tcaagttctg
     1561 gattgattgc acaatcacca aagatgtgtt gttcatcacc tttaatcata aagaagatac
     1621 ctgatgttct tgatacacct ggtttcgttt tgatgatttg taaagctgga cgcacagtgt
     1681 cgcctgttga atgtgctgca ccactaacta gaccatctgc tttaccagta taaacaagca
     1741 ttgtaccgaa gtagttcaca ttgtttaata attcttgtgc ttgttcttca gtcgctttac
     1801 ctttacgtcg ttcaacaaat gattgaacta attcagcttt caattcactt gtcgcaggat
     1861 taattaattc aatattagaa atatcaagat taagtttttg cgctaaagat tgaaccttag
     1921 tctcatcacc taacacgatt ggtgtaacat aatctgttgc ttgtaattgt gttgcagctg
     1981 ttagaacacg ttcgtcctct ccttcaggta atacgatttt aacgttttta ccagaaagtt
     2041 tgtcttttaa tacatttaat aaatcagcca taatgtcctc ctgtaatata aatcttatta
     2101 atcattcacg gtataattat acgccatttt atttcataat gccacgtcac caatttgtaa
     2161 tcataatttt atcgttaccc ctttgcttta tgataaaatt tataaagaac tgatgatttt
     2221 tgaaaaggag cgataaacat gagtcaagca gccgaaacat tagatggttg gtatagtcta
     2281 catttatttt atgcagttga ttgggcatca ttacgtctag ttccaaagga cgaacgcgat
     2341 gcacttgtca ctgaatttca atcattttta gaaaatacag caactgtaag atcatcaaaa
     2401 tctggtgatc aagctattta taatataact ggtcaaaaag cagatttgtt attatggttc
     2461 ttacgtcctg aaatgaagtc tttaaatcat attgaaaatg aatttaacaa attgcgcatt
     2521 gctgacttcc taatccctac atattcatat gtatcagtca ttgaattgag caattattta
     2581 gctggtaaat ctgatgaaga tccttatgag aaccctcata tcaaagcaag attataccca
     2641 gaattaccac attctgatta tatttgtttc tatccaatga acaagcgtcg taatgaaact
     2701 tataactggt acatgttaac tatggaagaa cgccaaaaat taatgtatga ccatggtatg
     2761 attggtagaa aatatgctgg caaaatcaaa caatttatta ctggttctgt agggtttgat
     2821 gatttcgaat ggggcgtaac attgttctca gatgacgtat tacaattcaa aaaaattgta
     2881 tacgaaatgc gctttgatga aacaacagca cgatacggtg aattcggtag tttctttgta
     2941 ggacatatta ttaacacaaa cgaattcgat caattctttg cgatttctta atacattgtt
     3001 acgtttataa attaataaaa aattccaagc ttatcggttt aagcttggaa tttttcgttt
     3061 atcttcagta tattcccgta tacataagac gtgatttggt aaatagttga aatctgtatg
     3121 tttaaactta tatatatatg tgctaatgta ttatcaataa caaagtacac tttgctcata
     3181 gcaaagtgta cccgagtagt cttccttggg agaactttaa ctactatcac tacatataaa
     3241 cgttaacctc aatagaaatt atacagtcgc tactctatac aatttttgta atggttaact
     3301 aatattattt taacctattt gaaatatttg aaacatattt ttgtcgaatt tttttcaata
     3361 atttttcctt tttatacttc aagagaattt taaccactaa aaattccgat gattattatt
     3421 acaatagtat caaatattag ttttttaaaa tcaataacaa cttatcaaaa agctaatgtg
     3481 gttattttat agtgtataaa ctataatgag tattaaattc ttataaacaa tggtgatgaa
     3541 atggacataa attcagaaga atacaaacaa gaggtactta taaaagatgt tgtcatgctt
     3601 gctgctcgcg tactattaga atctggtgca gaaggtacgc gtgtagaaga taccatgaca
     3661 cgtattgcaa aaaaacttgg ttacagtgaa agtaacagct ttgttacaaa cactgtcatc
     3721 cagtttacgt tacattcgga atcgtttcct agaatattta gaattacctc tcgagataca
     3781 aacttaataa aaatttctca agctaataaa atttcacgtc aaattacaaa caatgaaatt
     3841 tctttagccg aggcaaaaac acaacttgaa aaagtatatg ttgctaagcg tgacagcagt
     3901 cttcccttta aaggtttcgc tgcagcaatg attgcaatga gtttcttata tctacaaggt
     3961 ggtagattga ttgatgtttt aactgcgata ttagcaggta gtctaggata cctagtcact
     4021 gagattttag atcgtaagtt acacgcacag tttatcccag aattcattgg ttcattagtt
     4081 attgggatta tcgccgttat tggacataca cttattccaa caggtgactt ggcaactata
     4141 atcattgcgg cagttatgcc tattgttcct ggtgtattaa taacaaacgc aatacaagat
     4201 ttatttggtg gacacatgtt gatgttcaca acgaaatcat tagaagcatt ggttactgcg
     4261 tttggcatcg gtgctggcgt tggtagcgta ttaattttag tataggagta tcagactatg
     4321 ttttggatct taaactttat ctttagcttt ttagcttcaa tgttcttctg cgtcattttt
     4381 gatgcaccta gaaaattata tctatcatgt ggtttcgttg gtacgtgtgg atggatggtt
     4441 tacaccttat tcttcaacgg ctttaatgtg cacactatat actctagttt cttcggtagt
     4501 cttgccctag gtttattaag tcactatatg gctcgtaaac aaaaagaacc tgcaatcatt
     4561 tttatggtga caggtatcat cccgttagtg ccaggtggat tagcttacga tgcaacgaaa
     4621 aatttagtat tattaaattt cagtacagca atcaatacca tgctagaggt tacacttatt
     4681 gcaggagcca tcgcattagg tttattattc gccgaccaaa tttccaaatt aattgtttct
     4741 gggttcgtga aatcttttaa acgattataa atattgttag aaatggctca gcccaaactc
     4801 ttagcaatta tatatatggt ttaactatct cgtcttaaat gtcaatttat aatacaaaaa
     4861 atcgcacaat acgccttcga attaagaagg tatattgtgc aattctttta ttatttagtt
     4921 ttttcttttt caggttcttc aatcatatca tcaacttcat tttcacttct atttaaaatc
     4981 aagcgactta attcatcatt atccatattt ttcaatttag gatatcgaat cacaaagaat
     5041 attaaaccga tgattaacca tccaagtaat gcaatataag atggtgcagt cagtgctgca
     5101 ggagaacctg gcactaataa cagcgctaag aaaatgaatg atacaaatga gccgataata
     5161 gcaaacgttt tgtaaaccgg tgcatacgta ttactttgtt tgttataact gaataatttc
     5221 gcagcagaca aacatgtaat aaagtaggca atggatacac ctgtagatga catatctaca
     5281 atccaagtca atgccgttct tcctagccaa ggtgcaatta aagacacact tactaagaat
     5341 atgattgcga catatggtgt tttgtattta ctatgtaatt tactaaacat tgttggcata
     5401 atacctgaac gtcccataga aaataacaag cgacttgaac tcatcaagaa tccatttaaa
     5461 ccagtaaata tacccatcat aattgcaatt gctaatacac ctaatccaat ataaccaaat
     5521 gctgtttgtg tgacagcacc tgttaaccac aactgtccat ttaaactttg atgacttgtt
     5581 gataaccaac cagtgtataa aatcatgaca acataagtta atgatgctgc taataaactg
     5641 tacacgataa gcttaaatgt cttgtttggt gcaaagttaa actcttctgc tgtttgtgga
     5701 atattatcaa atccaacata tgcccatggt gccacggata caataaccac aatagacact
     5761 aaccatcctt tgctaggttc agctaacggt tgtaaatttt caagtgcaaa attattacca
     5821 aagaatgaac caaagaacat caataatacg acgattacca tcgccacaca gaaataatat
     5881 tgtaatgatc cagatacact tgcgccacga atcgttacta gcatgaatac aagtagtaat
     5941 acggtcgcaa taatgatttc cgtaatataa acgtcccagc ccgcaatggt gtatagtttc
     6001 ccattattta agacatctgg caataagaat ttaactagta aactgaatgc ggtcgcattt
     6061 aaagcaacga cacagacata accaaaagtt aaaaaccatg atgagaagaa actcacatat
     6121 ctgccgaaac ttaagaaact aaaggcaaac gcgccccctg atactggaaa tctctctact
     6181 aatgcgccat aactaaccgc aattaatatc attaataatg caccaataac aataccaatt
     6241 gatgctgcaa tcggacctga ctgcttaatc cagtctcctg gtaagatgaa tgcgccccat
     6301 ccgatacatg aaccatatgc aatcgcccat acaaactttt cagataggtt ttgttttaaa
     6361 tcgcctctat ctatttgctt attctttttt tccataaata aaaactcacc tcgaaggtat
     6421 tctataccca aaagatgagt ttgtaactct ttttcttgat tatttttatt aaaaatttaa
     6481 tgtttagtta cataggtaat ctaaagtttt aaaatttagc cagcaaattt gaatgtcgca
     6541 atgattaaca ttatccatcc aatgatgaac aatacgccac caattggcgt aatcgcacct
     6601 aaaactttaa tttgagttaa tactaaaata tataatgatc cactaaagaa aataatacca
     6661 gcaaatatta accagccagc ccagttaaca ttgattgaag ttgtaccact aattacacct
     6721 ataattaata atgctaagcc atggtacatt tgatacgtcg ttgctttttc ccatactgat
     6781 aaatagtgat cacttatttt tccttgtaaa ccatgcgcac caaatgcacc tgttccgaca
     6841 gccatcatcg cgtttaatgc acctaaaata ataaataatt tcattgctac ctctccttat
     6901 ataattcagt attaataaat gtttaaatcc gaaccctcga ataaaagtat atgcgtcttg
     6961 attattaacc tacctactta acaagctatt aagtaactat gtttcattgt tgctttttaa
     7021 aaatcaaata tggattcacc attgccaatg tcatcatctg tcgctaatct ttgttgttgg
     7081 tttgaaggtt gtgaatatga cttattatga tgatgtgctg aatgcgtatt tactttacca
     7141 cccatcgctt caatttcagc agctgtcact tgatgtgttg gctgcgtaat ttgtgattgt
     7201 ggcatttgat tatggttagc aatacgatga ttcatttgtt gttcaccaat atgtggtgtt
     7261 tttgatgttg aagcatataa agatgttaat atatgtatgg catacatatg tttttcaaag
     7321 tcatggtcat gctgcgcctg ctctgcttgt actaattctt gctcaattcg tgctattaaa
     7381 gtttctctat tcaccatcta cgcctcactt tcacaccaat taattggtga ttttcctact
     7441 gactctaaat aggtattcgc tttggaatac ggttttgatc caaagaaccc tctatatgca
     7501 gacagtggac taggatgcac tgattttata atacaatgtt tagatgtatc gataagcttt
     7561 attttttgct gtgcaggttt cccccacaaa ataaagacaa catgttcttt ataatcagac
     7621 actgctttaa taatttcatc agtaaatgtt tcccaaccaa tatcacgatg agaatttgct
     7681 tcaccctgtc ttacggttaa aactgtattt aataacaaga cgccttctct tgcccaatct
     7741 tgtaaatgtg gtgtttgtct aacacatcca atatcatctg ctaattcttt atacatatta
     7801 cgtaaagatg gagggaattt tgcgttaggt tgcactgaaa atgctaaacc atgtgcttgg
     7861 tttggaccat gatacgggtc ttgtcctaat ataacaactt tgatattttc aaacggtgtt
     7921 aaatcaaacg cttgatatat attttcccta tcagggtata cgattgcagt cgaatattct
     7981 ttttctaaaa aatcatgcat agctttaaag tcatgtttcg ttgttatgtc atgaaaaatt
     8041 tgcgaccatt ccattcaatc tcacctcagg acatatttta acatatcaca cttatctaaa
     8101 gtgaatgaca ttttcaaaag ttacaatagt atgaatgatt catttcaaca atcatcaaat
     8161 attgaatgct taatattttt caattaggtt aaaaatgata tgatgaatac aatattatta
     8221 tgtaggagtg aagctcaatg gctttaaaga aagttttaac aattgccggt tctgacacaa
     8281 gtgctggtgc aggtatgcaa gcagatttga aaacgttcca agaattagat acgtatggca
     8341 tggtcgcttt aactgccatc gttactatgg ataaagatac atggtcacac gatgtcacac
     8401 cattacctat ggacgtattt gaaaagcaac ttgaaacggc tttatcaatc ggacctgatg
     8461 ctattaaaac aggtatgtta ggtactgagg agataatcaa acgtgctgga gaagtatatg
     8521 aggcatctaa tgcacaatac tttgtagtag atcctgtcat ggtttgtaaa ggcgaagatg
     8581 aagtgcttaa ccctggaaat actgaggcga tgattaaata tttactacct aaagcaacgg
     8641 tagtgacacc aaacttattt gaagcaggtc aattatcagg attaggtaaa ttaaattcaa
     8701 ttgaagatat gaaaaaggct gcaacaatta tctttgataa aggcgcgcag catgtcatca
     8761 ttaaaggtgg caaagcttta gatcaagata aatcgtatga cttatactat gatggtcaaa
     8821 cattttatca actaactaca gacatgttcc aacaaagtta taaccatggt gctggttgta
     8881 catttgctgc ggcaacaact gcatatttag ctaacggtaa gtcaccgaaa gaagctgtga
     8941 ttagtgcgaa agctttcgtc gcttctgcta ttaaaaacgg ttggaaaatg aatgattttg
     9001 ttggtcctgt ggatcacggt gcatacaacc gtattgaaca tatcgatgtt gaagtaacag
     9061 aggtttaatg cttcgttcat aaaaaataga ttaaccacac cttatgattt gagtgttatt
     9121 gctgtgcgcc agtaaagtta gcttttaaag actaactttg gggctcagta cacaataaag
     9181 tcactttcgg tgtggttatt ttagttatta aatacttgtt tgattttcac agttaaattg
     9241 ggcaaataag ttttgtaagt catcgttata gatagtttta tttcaacata tgtatataat
     9301 attcatttac agtgtcacga atcagttatc ctaaaaaaac aaatcctttc ggaactttat
     9361 tatgcccttg tttaaacata acgttttatc ccgctctact aattctgcca tttttttaaa
     9421 attaaaaata acttctcaac gactttataa aaatatattc cttttaaaac atgatacata
     9481 aatagtctta gtaaatcata cgttctatgt ctgataaaaa gttgatactt aaggttgatg
     9541 tcacacataa aaatgttatt tataatataa ccatagagta gcaaaggagg taatataggt
     9601 tatgattatt tatcgacagt atcaccatga aggcgcacca gtttatgaaa ttataaccaa
     9661 aacgtttcag catgtttcaa ttaaatgtga cgattcattt agtgatactg aaatattcaa
     9721 attgctctct ttattacaag acgatataga tcatatgaaa gttagttaaa ttgactaatc
     9781 gttccaataa tccagaattt attaaacatt gatatagttg ttattattat tttagattaa
     9841 tgcatccccg tgcaaagaaa aaaacgtaac ctatatttat ctctgttccc aataataata
     9901 acaatacata actgatatta gctgtccttt catctcctaa ttaatatgcg ttcatgcagc
     9961 taatatcggt tattttttgt gctcaaagaa atctttattt taatctctcc gtcgtcatta
    10021 attgctatca tttcttgctc gtgttcttca atgaattcga tattttcatt tattaaaata
    10081 ggtttaacgt agttaatttg acactggttc gcctctaatg acatagccat cagcatcatt
    10141 tgactcacta attgtcctgg cacaatctca ttatgtattg gattatcatc atttacgagc
    10201 gccaaatatt catttatcca tatctcatta aacttcatct ctacttcatc gccttaataa
    10261 aagtttgtgt aaccgttatg acatgttgat catttttagt taatgttaaa gccattgtat
    10321 aacgtgtgat attacgaatc tgtcggcatt caatatcttc taaatgccct acatatattg
    10381 tgtctacttc tatcttttga gtttgattaa ttgctgattt tgttaaaatc agttcgctat
    10441 ttgcatattt tttaaataaa tcaaattttg accataatga cgcacacatt aatggtggta
    10501 cttcttttct ataaggttgg ttaaaacact gataatatgc agccacacta tccttagaaa
    10561 atgacacatt taatcgctgg ttagaatcga gtgaataatg ctgcatttcc tagacctccc
    10621 cctatcccca tagatgcaat cataatctct ttgtcaaaca tataaaataa tcgagtcact
    10681 aattgggcac cgcttgcacc gtatggatga cctgatgcta atgcaccacc ccatatattt
    10741 aattgcgtat ttgaaatatt taaagcttgt tggcaggcaa ccacctgtgc actgaacgct
    10801 tcgttaattt cgatgacttc aatattttct atcgttaatt gatttctttt tagtaagttg
    10861 gaaatggctg gtactggacc aatgccagga aaattagaat caacacctac cgtaacacca
    10921 tctttaaata ataaaccatg ctcgaaacct aattcgtatg ccatatcttt ttccataata
    10981 agcaataaaa ctgcaccatc atttttcata caactattcg cagcggtaac ggtcccacct
    11041 ttgatcacgg gcttaaatcg gccaaagtta tctttcggaa tatgtgattt tagactttca
    11101 tcagtgttga atatttctcc tttaacggtt ataggtaata tttcctgaga aatatttcca
    11161 ttctttacat tttccgctgt caattgatga cttcgataag caaattcatc ttgtaattct
    11221 cttgaaatat catacatctt ggctacattt tcagcacctt gaatcattga tgggtcgctc
    11281 atttcaggtg caaatgatgc acgctcataa aactcaggta atgctgtttc gtacacagaa
    11341 tgcggtcgtt tgattttcca aggtgctcga cttgtacttt caacaccgcc tgcaatatat
    11401 accttgccag ctccggcttg aatcatgcga catgcatatt gaacactttc aagtccagac
    11461 ccacattgcc gatcgattgt gacgccaggt attgaatctt tgagccccgc ttcaagcaat
    11521 gcttttcttg caatattgcc accattccca acaacattac ctaaaactac atcatctatt
    11581 ttagatatta cctctggata cttctcttta aaatgttgga ataaaggttt gagcaattgt
    11641 tctggctcta aatgttttaa agtgcctcca tatttcccaa atgcagttcg tttagctgca
    11701 actatgactg cttgattcat attataattc acctctcaaa tacatcgata tcattttttc
    11761 tctagcaatt ttaccacttg cagtgtaata catcttttct acatgatgaa tcttcgatgg
    11821 aatttcatag cgtttcactt tcttttttaa aaattgtttt acatttttat gtgtaagtgt
    11881 cacatcacca gaataaagca atacacctat ttgaccaaaa cgcttatttg gaataccgat
    11941 tataattgct tcatcaatgc tcgacgattg cgttaaatgg cgttcaacat gtgctggata
    12001 tatattttga ccaccaataa ttaacatatc atgttgtcgt cccgttaaat ataaatactg
    12061 ctcttttaca tagccattat cattagtaac aaaccattca tcattattta tacattgttc
    12121 gcttacatag ccactaaaca tcatattact ttttatacaa atagttccta taccattgtg
    12181 atcgtgattc gttgttttca attccacatt tggaaatagc acacctactg attcaactgg
    12241 tgcttgctga ttcaagttat agctgataaa actggtttcc gatgtaccaa aaaattcaat
    12301 caaatttatg tcatttgctt gattttttat ctttttaaaa atagaagaat gcagcttatc
    12361 tccactgcta aaaaatgatt ggattgtatg ttcattgttg taaactaaca ataatgattt
    12421 aatcatcgtt ggaacaagaa acatagcaac tttgtatgat gatattttac gacattgatt
    12481 aagtaacttt tcaggatgaa aagtagtctg tcctataaaa gtacgaccag aacttaaagc
    12541 aaacaataat gcatataatg ttaacgagta cgatagtggt ccgggggctg ctatggcatt
    12601 ttcatttttt aacatcaaca tttcattaac ttcaaaagaa gccaaccatg aatcttcatc
    12661 acgataatat gcttttggca gtccagttgt ccctgaagta aaaccaatat gtaataaatt
    12721 ggggtagtgc tgtaattgcg ttgaatcaat aaacatcggt gagtctatac tctgcatgag
    12781 acctgtatct tcaattacat ctttaatacc atatttttca atcaattgac gatgtatagt
    12841 actttgccat ttaccttcca tcatcatagg cactttatgt aaacgatgca aagctaaata
    12901 acaaataata ctttgcaccg gtgatttcat attaatagca acgacagggt taagtgaata
    12961 tttctgcaaa gattcaactg cgctagtgat gcgggcattt agttgactat atgtaattat
    13021 ttcatcatct atatgtaatg ctatgtcatt aggtttattt tgagtatgtg ttttcaactg
    13081 ttctaaaatt acgttcatca atatccctca ctcaatgtaa actttaaaat acatcttgtt
    13141 aacattatat aatgaataag tatttttatc aggtcatact atctatgata aaaatcaaac
    13201 ttaagcccta gataaatttg atactgtagt accatgtttt ttattagata agacgttact
    13261 atatgtatat agagaaaaga agttatcgca atcgcaataa ctgacacaaa taagtcaccc
    13321 aatctattta agtttgtgtt ctctataata atattgaagg ttagagtctc aatattaatt
    13381 gcgttggaca aaatactcta agacatattt acatcctaac atttcaatag caagagaatt
    13441 ttaagtattc aaagctaaac atatgcttta agtcccctct ttcccccatt gcgataatca
    13501 ttgcagtggg ggtttatgta tatacaaaaa ctttatttcg gttaattaca gttctcaatt
    13561 taaaaagcac tttaaagtta gtagcgttta ctagctttaa agtgctttta tacgattgat
    13621 tattacttat ttactgaaat ctctaatctt tacgttcttt aacccaccat aatgcttctt
    13681 ttgggtgttc tgcataataa gctctatctt gctcgttatc tacatttgga tacgaacctt
    13741 ttagagattt tcctgctgta cttaaatgta agaatgtaat aactaaaaag ccaataacac
    13801 tgattgctgt taaataatac gcaggtgcta atggatctcc agttttcgta actaaccaag
    13861 ttgcaactaa tggcgtcgta ccaccaaata tcgaaacaga gatattaaat gttactgata
    13921 aagttcgata tcttatatga ctataaaaca tcgttggtaa cgaccctggc attgtcgctt
    13981 cgtaagttga taagaaaaat cctaatataa atataccgat tactattaca acaaatgatt
    14041 gtgaatgtaa taacataaat gcgatgatac tgaataatgt tagcccacca gtaccaatta
    14101 gaaatacttt cttttcacct attttatccg ctaacttacc aaacattaat gctaatggaa
    14161 tcattattgc catgacacaa gtaattaata cacttgttgt cgttgcatct aatttaataa
    14221 cttgttctaa ataggttggt aaatatgcag ttaccatata gttcgtaaca ttgaagaata
    14281 caacagctac aaaacataca aatatatctt tgtaataaaa tctgatgatt tgtaaaaagt
    14341 taatgttatc tctttctggt tgtgttgcaa catcattttc gaaaactggt gattcttcaa
    14401 gcttacgacg taaatataag ccaaataatc ctaggaataa accaagtaag aatgggattc
    14461 tccaaccaaa tgatgccatt tgttcatctg ttaaaaagaa tgttaataca gcaatcataa
    14521 ttgaagcagc tatgtaacct gataatgtcc caatttctag tccactacct aatgagttac
    14581 gacgcttatc tggagatgac tccgcaacat atgtcattgc ccccgcatac tctccacctg
    14641 ttgaaaaccc ttgtagtact cttgcaagca ataatagtat tggtgcccaa agtccaattt
    14701 gatcatagct tggcaataat ccaatggtta atgttgaaaa tgccattaaa ataattgttg
    14761 atgttaatac aactttacgt ccatatttgt caccaataat accaaataca acaccaccaa
    14821 ttggtcttaa taaaaacgca atggctaatg ctgcgaaagt caacatttgt cgaatgtctg
    14881 cactctctac tggagagaag aagttcgctc caatgtacgc agttgtatat gcatagacac
    14941 caaaatcaaa ccattccatt gcattaccga taccggttgc aacaacggtt tttttggctt
    15001 tctttgcatc caccatgtta atattctctt tattaaaatc catgaattca tacactccct
    15061 tttgtatgtt tcataattat acggagttaa ttagttttgt caaatttata caaatataaa
    15121 cttttatata aatgcaaaag tatgtttgtt caaaaatttc actttatcga attaaattca
    15181 atgattttat accctcgtac atttgaatta aacaataata attcttaaat cattgaacga
    15241 ttaacttttt ggaattataa ctaaaaatag cgatagcata caacttcata taagtcatat
    15301 gctatcgcca ttttcgacat cgtatttaaa gtaagatatt caattgactg caagtaatat
    15361 gtcatcagtc ggagttattt gtattaatat caaataatta attacgcaga cttttctatt
    15421 taaacataca cgattattaa taaatattta ttttaaattc ctaatctaaa gcgaaatatt
    15481 tctat
//
LOCUS       DAHHRR010000055        15480 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.055, whole genome shotgun sequence.
ACCESSION   DAHHRR010000055 DAHHRR010000000
VERSION     DAHHRR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15480
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.055"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(28..957)
                     /locus_tag="NBI42_001499"
     CDS             complement(28..957)
                     /locus_tag="NBI42_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD1777919.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIVLNIFINPDVFSLHFFSNQTFNYTWFVGGLLGVGYLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIIGIVIMNQFNKNNLLLTDQKY
                     LLFWLLLGFIFGFFPPIQTTINSALASHTHSPAFASLVSFTIGSITLLILTAIFNRSL
                     KLKTSHLKFGKLKPIYFTGGILGMAFVTANIILMPHMGAALTTLIGMFGQILMGILID
                     HFGLFGSPKIAMTSRKTIGLLCILTGIILLRLF"
     gene            complement(1152..1376)
                     /locus_tag="NBI42_001500"
     CDS             complement(1152..1376)
                     /locus_tag="NBI42_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCD1777920.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(1393..1596)
                     /locus_tag="NBI42_001501"
     CDS             complement(1393..1596)
                     /locus_tag="NBI42_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777921.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYNNIELIKSLSI"
     gene            1755..2315
                     /locus_tag="NBI42_001502"
     CDS             1755..2315
                     /locus_tag="NBI42_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1777922.1"
                     /translation="MNKVEAIKFNDDIVKMYEALNIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIENVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            2334..4736
                     /gene="mnhA2"
                     /locus_tag="NBI42_001503"
     CDS             2334..4736
                     /gene="mnhA2"
                     /locus_tag="NBI42_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HCD1777923.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALIAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYVFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITDILGQADHIKNHGLFMPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLQFTPLLGLSNMYIYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGFSQHQQDVLAPI
                     YTFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKIFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVPALRAV
                     SAGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRTGLPKVHQLHVS
                     EFGPLEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            4723..5148
                     /gene="mnhB2"
                     /locus_tag="NBI42_001504"
     CDS             4723..5148
                     /gene="mnhB2"
                     /locus_tag="NBI42_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HCD1777924.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            5145..5489
                     /gene="mnhC2"
                     /locus_tag="NBI42_001505"
     CDS             5145..5489
                     /gene="mnhC2"
                     /locus_tag="NBI42_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HCD1777925.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            5479..6975
                     /gene="mnhD2"
                     /locus_tag="NBI42_001506"
     CDS             5479..6975
                     /gene="mnhD2"
                     /locus_tag="NBI42_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HCD1777926.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVRRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDVFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            6976..7458
                     /gene="mnhE2"
                     /locus_tag="NBI42_001507"
     CDS             6976..7458
                     /gene="mnhE2"
                     /locus_tag="NBI42_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCD1777927.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     AITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            7455..7757
                     /gene="mnhF2"
                     /locus_tag="NBI42_001508"
     CDS             7455..7757
                     /gene="mnhF2"
                     /locus_tag="NBI42_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HCD1777928.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            7732..8169
                     /gene="mnhG2"
                     /locus_tag="NBI42_001509"
     CDS             7732..8169
                     /gene="mnhG2"
                     /locus_tag="NBI42_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HCD1777929.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDDHTHASILLSSNEQNSIEALQLRAKKREEHRKKWYQND"
     gene            8509..10551
                     /locus_tag="NBI42_001510"
     CDS             8509..10551
                     /locus_tag="NBI42_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCD1777930.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(11445..12374)
                     /locus_tag="NBI42_001511"
     CDS             complement(11445..12374)
                     /locus_tag="NBI42_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCD1777931.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(12371..13207)
                     /locus_tag="NBI42_001512"
     CDS             complement(12371..13207)
                     /locus_tag="NBI42_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD1777932.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFIGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(13201..13944)
                     /locus_tag="NBI42_001513"
     CDS             complement(13201..13944)
                     /locus_tag="NBI42_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD1777933.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGQLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            14066..14710
                     /locus_tag="NBI42_001514"
     CDS             14066..14710
                     /locus_tag="NBI42_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCD1777934.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(14790..>15480)
                     /locus_tag="NBI42_001515"
     CDS             complement(14790..>15480)
                     /locus_tag="NBI42_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HCD1777935.1"
                     /translation="VAACYIAIHQYRHKPVLNYLDVILNYIPVLTHEFGHVLFNKLAG
                     GRAKDLVIVTNPRERQQTLQQGFAITQSRHLAGQWLTTIGGYFMPPIMLLIGLASSHY
                     QIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIMLYFILKDENIVEIQLLVTMSY
                     QYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKELSHVPIFIYSTIWIIFNLYAV
                     NILLKYTHLIN"
BASE COUNT         5141 a         2230 c         2662 g         5447 t
ORIGIN      
        1 agttaaaatg ataagctaaa agttaattta aaataatctt aaaagtataa tgcctgtcaa
       61 aatacataat agaccaatag tttttctgga tgtcattgct attttaggtg aaccaaataa
      121 tccaaagtga tctatcaata tgcccattaa aatctggcca aacatcccaa taagtgttgt
      181 taatgctgca cccatatgag gcattaagat aatgttagct gttacaaaag ccatgccaag
      241 tataccgcca gtaaaataga taggttttaa tttaccgaat tttaaatgac ttgtttttag
      301 ttttaaagaa cgattaaaaa tagcggttaa aatcaatagc gttattgacc caattgtaaa
      361 tgatactaat gatgcaaagg ctggtgaatg agtatgactt gctaaagcac tattaattgt
      421 cgtttgaata ggtggaaaga aaccaaaaat aaatcctaag agaagccaaa acagtaaata
      481 cttttgatca gttagtaata aattattctt gttaaattga ttcattatga cgatgccgat
      541 aatgagtaac aatactccaa ttgctttaat taaattaaaa tcatgaattg tagcgccaaa
      601 taagccaaat gtatcaataa tgacacccat aataatttga cccgcaactg ttgcaatgac
      661 agttaatgtt gcacctaatt ttggcaataa caataaattg ccagttaaat agccaacacc
      721 gagtaatccg ccaacgaacc aagtatagtt gaatgtttga ttactaaaaa agtgtaatga
      781 gaaaacgtca ggatttataa aaatatttaa gacgattaag catatagtac caactgaaaa
      841 tgaaatgaat gaagtataga aaggcgattt agtatacagt gatagtcttg aattgacaga
      901 tgtttggata ggaataagca ttccaactag gacaccaatg atatagaaaa gaagcatatg
      961 aataaagtct cactttccaa agttaataat aaaatcatat gtatacatta tcattataga
     1021 gaaaatatac aactcattta caataatgtt gaaaaaaaca ggacattaac acaacaaaat
     1081 caccctagac aatcatgatg aaagtctagg gtgttagtat ttatactaaa ttaattgcgc
     1141 tttcttttta tctatttctt atcttcaaat aaaacagttg ttgttttatc aataatttga
     1201 gcagaatgta ctttggtgat agtaccacta tttgatttta taatatttaa atctactaat
     1261 gcattcatac tatcacgtgc aacttgctcg gtcactacgt tgtttaattg tggtaattgc
     1321 agttttacag gcttgtctag tgaagattta aaacttaatt caagtgtttt agtcatgaat
     1381 atgtcctcct gattaaattg ataaagattt gatgagttcg atgttgttgt atgtttctcc
     1441 agtaagtcgc tcgataagtt tgctgaatgt tttaatttgg tcgtttgatg catcagggtt
     1501 aatgttggcg aatcgacgct taaattctgt ttgtttgccg ttagcgtcta cttttgtaaa
     1561 tgataataca atagtgatgt ggtttatttt actcatattt taaaacctcc tttcacacta
     1621 tatatcgaaa taaaataata aaatggctaa ttttattttc tatgtttaaa atctataaaa
     1681 aaggtaatag atatatgtaa ctaaaatata aaatagctat gattaagaca cttaaagcaa
     1741 ggggaggttg agggatgaat aaagtagaag cgattaaatt taatgatgat attgttaaaa
     1801 tgtatgaagc gctcaatata aaatctgaac gtgactattt attctttaag ttagctatac
     1861 atagtggatt gaaagtatca gaattattaa caattacagt ctctcaagtt aagagactaa
     1921 ttgaaaaatg tacgttatca gaaatgtgta aagcacattt tcattcgttg attaaaatta
     1981 ggttaccaga aacattatcg aaagaactac ttcaatatat agaggacagg agtctttcga
     2041 atgaagacgt tctttttcaa tcactacgaa caaatcaagt attatctaga cagcaagcat
     2101 atcgaattat tcatcaagca tcaattgaag ctggcataga gaatgtagga ctaacgacat
     2161 tgcgtaagac atttgcatat catgcttatc aaaaaggtat acctatacca gtcattcaaa
     2221 agtatttagg gcatcaatct gctattgaaa cactaaattt tatcggttta gaaaatgagt
     2281 gtgaacatag tatttatatt tcattacaat tatagaaaca aaggaggcta ataatgagtt
     2341 tggtttattt attaattgcc atacttgtga ttatggcgat gatacttcta atgtctaaac
     2401 gtagagcatt ggctaaatat gccgggtaca tagcgttgat tgcacctgta atttcatcta
     2461 tctatttttt gattcaaata ccatcagtag ctaaactgca atatctttct acctctattc
     2521 catggattaa gacattagat atcaatttag atttacgttt agatggttta agtttaatgt
     2581 tttctcttat tatttcactt attggaattg cagtattctt ctatgcaact caatatttat
     2641 cctctcgaaa agacaattta ccaaggtttt atttttattt aacgttattt atgttcagta
     2701 tgattggtat tgtattatca gacaatacga tattgatgta cgttttttgg gaattaacga
     2761 gtgtatcatc atttttattg atttcatatt ggtataacaa tggtgacagt caatttggtg
     2821 cgattcaatc atttatgatt acagtatttg gcggattggc gttattagtt ggttttatta
     2881 tgctgtatat catgacagga acgaataaca tcactgacat attaggacaa gcagatcata
     2941 ttaagaatca tggattgttt atgcctatga tttttatgtt tttattaggt gcatttacaa
     3001 aatcagcaca atttccattt catatttggc tgcctagagc aatggctgca cctacacctg
     3061 taagtgctta tttacattca gccacgatgg taaaagctgg tatcttttta ttacttcaat
     3121 ttacaccatt attaggtctt agcaatatgt acatatatat cgtcacgttt gttggtttaa
     3181 taactatgtt atttggttca atcacagctt taaaacaatg ggatttaaag ggaattctag
     3241 catattcaac aatcagtcaa ctaggaatga ttatggcaat ggtcggtatc ggtggtgggt
     3301 tttctcagca tcaacaagat gtacttgccc ccatttatac atttgtatta tttggtgcgc
     3361 tatttcatct aatgaatcat gccatcttta aatgtgcgct tttcatggga gtaggtattt
     3421 tagatcatga agcaggttca agggatatac gaattttaag tggaatgcgt caactatttc
     3481 ctaaaatgaa tctagtcatg acgatagcgg ctttatctat ggctggagta ccatttttaa
     3541 atggattttt aagtaaagaa atgttcttag atgcattaac acaaactgga caattatccc
     3601 aattcagttt gatttcaatg atagctatcg tgtttgttgg tgttattgcg agtgttttta
     3661 cattcacata tgcactatac atggtaaaag aggtattttg gacaaaatat gattctaaga
     3721 tttttactaa aaaaaatatc cacgaaccat ggttgtttag tttaccatct cttatattaa
     3781 tggtgctagt acctgtaatc ttttttgtac caaatatatt tgggaagggg attatcgttc
     3841 cagcattaag agctgtatca gctggtaatc atcaaattga tcaattggca ccacatgttt
     3901 cgcaatggca tggatttaac ataccgcttc ttttaaccat catcattatt ttattgggta
     3961 gtgtactagc aatcaaagta gattggaaaa aagtgttcac gggtaaaatc agacagattt
     4021 cagtttcaaa aagctatgag atggtatatc gacattttga aaagtttgct acgaagcgat
     4081 ttaaacgtgt tatgcaagat cgtttaaacc aatacattat tatgacctta ggcatattta
     4141 tgattatcat tggatatggt tatattcgaa ctggacttcc taaagtacat cagttacatg
     4201 tttccgaatt tggaccgtta gaaattatat tagcaatcgt aactgtcaca attggtattt
     4261 ctttaatttt tatacgtcaa cgactaacaa tggtaatttt aaatggagtc atcggttttg
     4321 ttgtgacctt attctttata gcaatgaaag cccctgattt agcattaact cagctagtag
     4381 ttgaaacaat aacgacgata ctatttattg tcagtttttc aagattacca aacgtgccaa
     4441 gatctaacgc taacaaaaaa agagaaataa ttaaaatttc tgtatcactc ttgatggcac
     4501 ttattgttgt atcattaatt tttattacac aacaaacaga tggtttatca tcaatatcag
     4561 acttttattt aaaagctgac aaactaacag gtggtaaaaa tattgtaaat gcgatacttg
     4621 gtgactttag agcattagat acattatttg aaggattagt gttaattatt actgggctag
     4681 gtatttacac attattaaat tatcaagatc ggaggggaca agatgaaaga gaatgatgtc
     4741 gtgttaagaa cggtcacgaa acttgttgta tttatattat tgactttcgg attctatgtc
     4801 ttcttcgcag gtcataataa tcctggtggt ggctttattg gtggtttaat atttagctca
     4861 gcgtttattt taatgtttct ggcttttaat gttgaagagg ttttagaaag tttaccgatt
     4921 gattttagaa ttttaatgat tattggagca ttggtatcat ctattactgc gataatacct
     4981 atgttttttg gaaaaccatt tttgtctcaa tatgaaacaa cttggatact tccaatttta
     5041 gggcaaattc atgtaagtac aataacactt tttgaattag gtattttatt ctcagttgtt
     5101 ggtgttattg tcacagtgat gttgtcgctt agcggaggtc gatcatgaat ttaatattat
     5161 tactagttat aggattttta gtgtttatag gaacatatat gattttatca atcaatttaa
     5221 ttcgtattgt aatcggaatt tcaatatata ctcatgccgg taatctcatt attatgagta
     5281 tgggaactta tggttctagt agatcagaac cattaataac tggtggaaac caattgtttg
     5341 ttgatccctt gttacaagct attgtactaa ctgcaatagt tatagggttt gggatgactg
     5401 cgtttttact tgtacttgtt tatagaactt ataaagtaac aaaagaagat gaaattgaag
     5461 gcctaagggg ggaagatgat gctaagtaac ttattgattt taccaatgtt attaccattc
     5521 ctttgtgcct taatccttgt atttttaaaa aataatgacc gtatttctaa atatttatac
     5581 ttaggtacaa tgactatcac cacaattatt tcattaatgc tattaattta tgttcggcgt
     5641 caccgtccaa ttacgctaga cttcggagga tggtcagcgc cctttggtat acagttttta
     5701 ggagattctt taagtttaat tatggttaca accgcttcgt ttgtgattac tttaattatg
     5761 gcatatggat ttgggcgtgg cgaacataaa gcaaatcgtt atcacttgcc atcgttcata
     5821 ttatttttaa gcgttggcgt gataggctct tttctaacat cagatgtatt taatttatac
     5881 gtcatgtttg aaattatgtt actagcgtca tttgtactca ttacacttgg acaatctgta
     5941 gaacaattac gtgctgcaat tatttatgtt gtcttgaata ttattggttc atggctattc
     6001 ttattaggta taggtttact ttataaaaca gtaggtacat taaacttttc acatattgca
     6061 atgcgtttga atgatatggg agataatcgc actgtaacaa tgatttcatt aattttccta
     6121 gtcgcattta gtgcgaaagc agcgctggtc ctttttatgt ggctaccgaa agcctacgct
     6181 gtgttaaata ctgaacttgc agcattattt gcagcgttaa tgaccaaagt aggggcctat
     6241 gcattaattc gattcttcac tttactattt gatcaacata atgatctcat acatccattg
     6301 ctagcaacta tggctgctat aactatggtc atcggcgcta taggtgtcat tgcttataaa
     6361 gatattaaaa agattgcagc ttaccaagtc ataatctcaa taggatttat cattttaggt
     6421 ttaggaacaa acacgtttgc aggtattaat ggtgcaatat tttatttagt aaatgatatt
     6481 gttgtaaaaa cattgctatt ctttattatt ggtagtttag tttacattac aggttatcga
     6541 caatatcaat atttgaatgg cttagctaaa aaagaacctt tatttggagt tgcgtttatt
     6601 ataatgattt ttgctattgg cggcgtgccc ccatttagtg gctttccggg gaaagtactt
     6661 attttccaag gtgcattgca aaatggcaat tatattggac tagcgttaat gattattact
     6721 agtctaattg caatgtacag tttatttagg atactttttt atatgtattt tggagataaa
     6781 gatggggagg aagttaattt taagaaaatc ccactatatc gaaaaagaat tttaagtatt
     6841 ttagtagttg tggttatcgc aatcggaatt gctgcgcctg ttgtgttaaa tgttacaagt
     6901 gatgcaactg agttgaacac gagtgatcaa ttatatcaaa aacttgtaaa tccgcatttg
     6961 aaaggagagg actaaatgaa ccaaatagtt ttaaatatta tcattgcatt cttatgggta
     7021 ttatttcaag atgaagatca ttttaaattc tcgactttct tttctggata tttaattggt
     7081 ttaattgtca tttatatatt acacaggttt ttcagcgatg atttttatgt tagaaaaatt
     7141 tgggtagcta ttaaattttt aggtgtttat ttatatcaat taataacatc tagcattagc
     7201 acgattaatt atattctttt taaaacaaaa gatatgaacc ctggattact ttcatatgaa
     7261 acaagactaa ctagtgactg ggcaataaca tttttaacaa ttttaattat tataactcca
     7321 gggtctacag taatacgaat ttctcaagac tctaaaaagt tttttattca tagtatcgac
     7381 gtgtcagaaa aagaaaaaga tagtttgtta agaagtatta agcattatga agacttaata
     7441 ttggaggtgt cgcgatgata caaacaataa cacatattat gattattagt tcactcatta
     7501 tttttggaat tgcattaatc atctgtttat ttagattaat caagggacct acaacagcag
     7561 atcgtgtcgt tacatttgat acaacaagtg ctgtcgtaat gtcaattgtg ggtgtgttaa
     7621 gtgtacttat gggcaccgtt tctttcttag attcaatcat gctcattgcc attatatctt
     7681 ttgtaagttc tgtttcaata tcacgcttta ttggtggggg gcatgtgttt aatggaaata
     7741 acaaaagaaa tctttagtct tattgctgct gtgatgttgt tgttaggtag ttttattgct
     7801 cttattagtg caataggtat cgtgaaattc caagatgttt tcttaagaag tcatgctgca
     7861 acaaaaagtt cgactttatc cgtgttatta actttaatcg gtgtattaat ttattttatt
     7921 gtgaatacag gatttttcag tgtgcgttta ttactatcac ttgtttttat taatttaact
     7981 tcacccgtcg gcatgcactt agtcgctcgc gctgcttatc gcaacggcgc ttatatgtat
     8041 cgaaaaaatg atgatcacac acatgcatca atattattaa gttcaaatga acaaaactct
     8101 atagaagcat tacaattacg tgctaaaaaa cgagaagagc atcgtaagaa atggtatcaa
     8161 aacgattgaa gtgttggcta aattgttaag cataacaatg cttttgaaga tttgttttca
     8221 aaattaatca ttaaaagagt actttgaaat tggctagtcg attttaaggt gctcattttt
     8281 tgtattataa gttaatgttg ttatgtgaaa gcaagttgtt tatttataca aacaattagt
     8341 gataaaataa aattaaataa ttgcaaattt aagtactgca atcgtagaca tatatctata
     8401 ttaaagtgct tttgtggtcg ctaactcaat ttatcctcta aagtataatt acaacgagat
     8461 gtgatactta attttcaatt aaacaatact ttttagagag gtgaaagttt ggaaatattt
     8521 gaaacaattc ttatatttat agctgttgtg atactaagtt cgtttgtcca tactttcata
     8581 cctaaagtac ccctagcatt tatacaaatt ttcttgggca tgttactatt tattacccca
     8641 atccctgttc aatttaattt tgattctgaa ttgtttatgg taacaatgat tgcgcctttg
     8701 ttatttgtag aaggtgttaa tgtttctaga gtccatttaa ggaaatatat taagccagtg
     8761 atgatgatgg cattaggatt agtcattact actgtgatag gtgtaggttt atttattcat
     8821 tggatttggc cagatttacc tattggagca gcatttgcaa ttgctgccat tctttgtcct
     8881 actgatgcag tagcagtgca agcaatcact aaaggaaagg tcttgccaaa aggagcaatg
     8941 acaattcttg aaggtgagtc attattgaat gatgctgctg gtattatttc atttaaaata
     9001 gctgttggag tattagttac aggtgctttt tcacttgttg atgctgttca gttgttttta
     9061 attgcatcaa ttggtggcgc agtggttggt ttacttatag gtatggcatt agtaaggttc
     9121 cgattaacat tgatgcgtcg aggatatgaa aacattaata tgtttacaat tattcaattg
     9181 ttaacaccat ttgttacgta tttaattgct gaattgtttc acgcatcagg aatcattgcg
     9241 gcagtagttg caggacttgt acatggtttc gaacgtgaca gaattatgca agtacgtaca
     9301 caactacaaa tgagttacaa tcatacatgg aatatactag gttatgtttt aaatggcttt
     9361 gttttttcaa tattaggatt tttagtacct gaagttatta ttaaaattat caaaacagaa
     9421 ccgcacaatt taatcttttt aataggcatc actattgttg ttgctttagc tgtctatcta
     9481 tttagatttg tttgggttta tgtcttatat ccttattttt atttagccat cagtccattc
     9541 caaaaaatga tgactaaaaa tgatgatgat aatccaacaa ctgagaaacc accaaagcga
     9601 agtttatacg ctttaattat gacgttatgt ggtgtgcatg gaacaatttc tttagcaatc
     9661 gcattaacgt taccgtattt tttagcaggt catcatgctt ttacgtatag aaacgactta
     9721 ttatttattg catctggtat ggttattatt agtttggtag ttgcacaagt attattgcca
     9781 ttattaacga aacctgcacc taaaacagta attggcaata tgtcgtttaa agttgctaga
     9841 atttatatat tagaacaagt tattgattat ctaaatcaaa aatctacttt cgaaacaagt
     9901 tttaaatatg gtaacgtgat taaagaatat catgataaat tagcattttt aaaaactgta
     9961 gagaaagatg atgaaaactc taaagaatta gaacgtctac aaaaaattgc ttttaatgta
    10021 gaaacaaaaa cattagagtc tttagtagat gaaggacaaa taacgaatag tgtacttgaa
    10081 aactatatgc gttatgctga aagaacacaa gtatatagac aagcatcatt aataagaaga
    10141 atgattgtat tattacgagg tgctttatta aaacgaagag tacaaacgag agtgaactcg
    10201 gcatcttcac ttagtgttac ggataactta atggaattaa ataaaattaa taaattagtc
    10261 cattataatg tggttagtcg tttgtctaag gaaacaacaa aagataatac acttgaggtt
    10321 ggaatggttt gtgacggtta tttaatgcga attgaaaact taacaccatc taatttcttc
    10381 aactcagcaa gtgaagatac gattactaaa attaaattaa atgcattgag agaacaacgt
    10441 cgcattttac gtgagttgat tgatacagat gaagtatcag aaggtacagc gttaaaacta
    10501 agagaagcca tcaattatga tgaaatggtt attgtagata gtatgacgta gttcgtaatt
    10561 atgctaaaag ggattgatga aagactgaat ggcttttcat caatcccttt tattttaggg
    10621 gaactgaata gatagtttta aactatacga attattaata tttgagattt aattgaaata
    10681 agttttaaaa attggaggag atagattaat cgaagtcatt taaagatgaa gttaagtaca
    10741 ttcacaaaaa atagccacac tcatatgaca tcggatgagt gtggcttaag gatctatggg
    10801 gggaggaaac catagatgtt tactttgata ggccagatta aatatcaaag tatgcgatta
    10861 tttatagctt gatgcaaaag tggtatgcct attaaaagtt actgcacata gcttttaata
    10921 ttccgttcaa aggaaagggg catacaattg aacaatctgt aatagtactt ttaaccagct
    10981 atgctaaaag tctagtaggg agaacagttg tccaatcaca taagaacctc taacttcgtt
    11041 agtacgatta agaaaagctt tttagttagt atgtaataca atttattgac gcgcgtgaat
    11101 ctcttttata agagtgtgta gggaatggcg ttgtataaat tgtattagaa gaacttctaa
    11161 cgcatctctg tggttaaaag agatgaaggg aacgacagtt taattaaaac tgcataagaa
    11221 cttctagctt ttctctttcg ttcaaagaga agttctaata ccaccatatc gtgcgatcgg
    11281 gaacggtata tatattaata ggagggtaat atatatttaa cgcacgatat gggactatta
    11341 gccttcgact ttgttatgtt gatgtgtggc ctaaaatatt ggagatacca atattttagg
    11401 ttgcatcaac atcaattcat cttacttcat taaaacacag cgtgttattt catgcttccg
    11461 tgtacagttt caatatttga tttcatcatt ttgtagtaag agtcaccttt agtgccttct
    11521 ttaccgattg aatctgtgta cacttcacca aagatatctt tcttcgtttc ttcagataaa
    11581 ctttccattg ctttcttatc aacacttgtt tctactaata agtgttttaa tttgtgcttt
    11641 ttaacaaact caatagcttg tctcatttgt tcaggtgtac cttgtttttc agtgttaatt
    11701 tcccaaatat aacctggtgt aataccgtat tgttttgaga agtacttgaa ggcaccttca
    11761 cttgtaatca tggcacgttg ttcttttgga atgtcattaa atttgtcttt actgtcatta
    11821 tttaattttt ccaattgagc aatgtatttg ttaccttgct tttcataatc tgctttatgt
    11881 tttttgtcgt tatcgataaa tgtttgttga attgttttta cgtatttaat accgttatct
    11941 aaacttaacc atgcgtgtgg atcttgttta tctttgttgc cttcttcacc gtttaaatag
    12001 ataggtttaa catcttttga tactgcgata acttttttat cttttaatga tttaccagcc
    12061 tgttctaagg ctttttcaaa ccaaccgtta ccagtctcta aatttaatcc attgtataaa
    12121 ataacgtcag cgtcagttaa ctttttaata tctttaggtt taacttcata ttcatgagga
    12181 tcttgaccaa caggtacaat actatgaata tcgacgttgt ctccaccaac atttttagcc
    12241 atatcatata aaattgaatt cgtcgttact acttttaatt tgccatttga cttatcactg
    12301 ctttgtttac caccagtacc acatgcagca actagaagta ataaggctaa taataaaggt
    12361 actaattttt tcatgttaaa cttcctcgtt tctttctatt cgtaaatttt gtgaaaaata
    12421 atgtgatgat ataaattaca aacgtacaaa gtacgattgt cgcaccacta ggaatgttgt
    12481 aaatatagct gtaataaagt ccgacaattg aacttatgac acttattaaa cttgctataa
    12541 tcatcattga gtatagtttt ttactaatta aaaatgctgt agatgcaggt gtaattaata
    12601 gtgcaactac aagaataata cctaccgttt gaatacttgc tactgttact aatgagagta
    12661 acaacatcac aaagtaatgt aataacgtcg tatttagacc actcattcta ctaaacgttg
    12721 gatcgaatgt agaaatcatt aatggacgat agaaaataat gattagaata aggacgattg
    12781 aaccaatcac aatagttgtt aaaaatgcac tatttgtgat tgccagtaaa ttaccaaaca
    12841 gaatatggta caaatctgtc gtagtgttta ttaagctaat aataataatc cccgaagcta
    12901 agaaagcggt aaaactaatt ccaatagcgg cgtcaggttt cgttttacta ctagatgtga
    12961 tataaccgat aaaaatactt gcgatcatac cagttataag tgcgcctata aacattggaa
    13021 taccaaataa gaatgatagg gcaacaccag gtaatactgc gtgactcatt gcatctccca
    13081 ttaatgaaag accacgtaat acaattaaac taccaactgt accacaaact atccctacaa
    13141 taattgaagt tatcaatgct cgattcaaga attgatatgt aaataaatgt tcgacaaact
    13201 ctaacatgtt atattgctcc tttgactagg gtcactacag tcagtgctac tcataaatgt
    13261 ttcgtttaag cgagtgacac tcatagcctc ttcactatca ccaaaatatc gtaatgtttg
    13321 atttaataga ataatgcgat caaagtattg ctttgctttt gataaatcat ggtggatgat
    13381 aagaataagt tgtccttgtt gttttaagtt ctcgattttt gtcatgatta atttttcgct
    13441 actaaaatca attccgacaa acggctcatc tagaaaataa acttcacttt cggacatcaa
    13501 tgctcttgct actagcacac gttgtaattg tccaccactt aattctgaaa tttgtcgatg
    13561 acgtaaagat tctaattcta aatcgcttaa taactgtttg agtttatccc ttgctgattt
    13621 attaggtcgt ctaaaccatc caatttcttt gtagcaacct gataaaatca cttgttccac
    13681 acttatagga aaatctaaat caatatgtgc tttttgtgga atatatgtaa tatgttgcag
    13741 ttgttgttgt ataggtttgt tatataacaa tttagtaccg gtagcattaa attcaccaat
    13801 taaagacttg ataagggaag atttaccagc accattcggg cccatgatac caattatttc
    13861 gccgcgtaca ggtatcgata aggaaatgtt tttaagtaca tgcttattac ctaaaaacag
    13921 atttaaatct tttgtttcta acaaacgttt atacctccta attaaaagtt taggctaacc
    13981 taattaattg tataataaac tgagaatatt tatcatgtca agtaaattcg tgatataata
    14041 tagacaatgt atgtgaggtg aaagtatgtt aactgaagaa aaagaggact atttaaaggc
    14101 aatccttacg aataatggcg ataaaaactt tgtgacaaat aaaatcttat ctcaattttt
    14161 aaatattaag cctccatctg taagtgaaat ggtaggacgt cttgaaaaag caggctatgt
    14221 tgaaacaaaa ccgtacaaag gtgttagatt aacagaggat ggtttaacgc atacgcttga
    14281 tatcattaag agacatcgac tattagaatt atttttaata gaaatattga aatataattg
    14341 ggaagaagta catcaagaag cagaaatttt agaacatcga atttcagatt tatttgttga
    14401 aaggctggat agcctgttaa atttcccaga aacttgcccg cacggcggtg tgattcctag
    14461 aaataatgaa tataaagaga aatatataac aacgattttg aattatgaac ctggtgatat
    14521 cgttacaatc aaacgtgtga gagataagac cgatttgcta atatatttgt ctagtaaaga
    14581 catctctatt ggtaatgaag tggaaattgt atcgaaagat gaaatgaata aagtaattat
    14641 cattaaacga aatgataatg taattattgt tagttacgaa aatgcaatga acatgtttgc
    14701 tgaaaaataa aataaagaag ccataaagat atccatgatt gtactgataa agacatatgg
    14761 ataattgctt taggcttctt ttttattagt taatttatca agtgagtata tttgagtaaa
    14821 atattcactg cataaagatt gaagataatc cagattgtac tataaatgaa gataggtaca
    14881 tgactgagtt ctttaagtgc actaccatcc cactgtggac tcggacgctg gaaagtcaat
    14941 ttagcaatcg tccaactaga ttgtagaact tcgcctaata atacacctaa aatatattga
    15001 taactcattg tgacaagtag ttgaatttct actatatttt catcttttaa tataaaatac
    15061 aacatgatag aaattaaagt tataacaaca atgggtgagc cttttctaga tgttaaaatt
    15121 aaaaaataaa taaatatcaa taaataggta aatataaaga aactaggtat ctgataatgg
    15181 ctcgacgcta aacctatcaa taacataata ggtggcataa aataaccacc aatcgttgta
    15241 agccattggc ctgctagatg tctagattgt gtaattgcga atccttgttg taatgtctgt
    15301 tgtcgctctc gtggatttgt tacaatgact aaatcttttg cacggccacc agcgagttta
    15361 ttaaacagta catgaccaaa ttcatgtgtt aaaacaggga tatagtttaa aatgacatct
    15421 aaatagttca aaacaggctt atgtctatat tgatgaatag caatataaca agctgcaaca
//
LOCUS       DAHHRR010000056        15101 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.056, whole genome shotgun sequence.
ACCESSION   DAHHRR010000056 DAHHRR010000000
VERSION     DAHHRR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15101
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.056"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..1064)
                     /locus_tag="NBI42_001516"
     CDS             complement(<1..1064)
                     /locus_tag="NBI42_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCD1777936.1"
                     /translation="MKIADLMALLDHHVPFRTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPHGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDACQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFATDIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGTDVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKTLLMNWFNTEKINIDVEA"
     gene            complement(1067..1744)
                     /locus_tag="NBI42_001517"
     CDS             complement(1067..1744)
                     /locus_tag="NBI42_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCD1777937.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCDCGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIHPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTESLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     QLIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQLNEEI
                     SVIERVL"
     gene            complement(1875..2981)
                     /gene="rpoD"
                     /locus_tag="NBI42_001518"
     CDS             complement(1875..2981)
                     /gene="rpoD"
                     /locus_tag="NBI42_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCD1777938.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(3205..5004)
                     /locus_tag="NBI42_001519"
     CDS             complement(3205..5004)
                     /locus_tag="NBI42_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCD1777939.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYSLNDEPYENEIDDYVNVINENGQETIE
                     SLNHKLREATRIGDVELQKHYLQQIVAKNKERM"
     gene            complement(5065..5883)
                     /locus_tag="NBI42_001520"
     CDS             complement(5065..5883)
                     /locus_tag="NBI42_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCD1777940.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(5894..6517)
                     /locus_tag="NBI42_001521"
     CDS             complement(5894..6517)
                     /locus_tag="NBI42_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1777941.1"
                     /translation="MELSQRQERIIEIVKTQGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGSDIHTVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            6852..8243
                     /locus_tag="NBI42_001522"
     CDS             6852..8243
                     /locus_tag="NBI42_001522"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HCD1777942.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(8394..9140)
                     /gene="recO"
                     /locus_tag="NBI42_001523"
     CDS             complement(8394..9140)
                     /gene="recO"
                     /locus_tag="NBI42_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCD1777943.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYASLAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDIHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SEIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(9168..10067)
                     /gene="era"
                     /locus_tag="NBI42_001524"
     CDS             complement(9168..10067)
                     /gene="era"
                     /locus_tag="NBI42_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCD1777944.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(10068..10472)
                     /gene="cdd"
                     /locus_tag="NBI42_001525"
     CDS             complement(10068..10472)
                     /gene="cdd"
                     /locus_tag="NBI42_001525"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCD1777945.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSHFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(10483..10827)
                     /locus_tag="NBI42_001526"
     CDS             complement(10483..10827)
                     /locus_tag="NBI42_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCD1777946.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(10830..11297)
                     /gene="ybeY"
                     /locus_tag="NBI42_001527"
     CDS             complement(10830..11297)
                     /gene="ybeY"
                     /locus_tag="NBI42_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCD1777947.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIKDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPDIDFSGFDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(11298..12245)
                     /locus_tag="NBI42_001528"
     CDS             complement(11298..12245)
                     /locus_tag="NBI42_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCD1777948.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGCEQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            complement(12549..13247)
                     /locus_tag="NBI42_001529"
     CDS             complement(12549..13247)
                     /locus_tag="NBI42_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCD1777949.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(13264..14253)
                     /gene="floA"
                     /locus_tag="NBI42_001530"
     CDS             complement(13264..14253)
                     /gene="floA"
                     /locus_tag="NBI42_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HCD1777950.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDELPEH"
     gene            complement(14271..14954)
                     /locus_tag="NBI42_001531"
     CDS             complement(14271..14954)
                     /locus_tag="NBI42_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCD1777951.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFIYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
BASE COUNT         4680 a         2833 c         2071 g         5517 t
ORIGIN      
        1 gcttcaacat ctatatttat tttttctgta ttgaaccaat tcattagtaa cgtttttaaa
       61 ccttctttca tcacatattc gctgtaatga ttaatatcaa ttaagttcac accatgaatt
      121 ttagcatcta aggcatcatg atgtttaata tcacctgtaa caaagacatc tgtgccttgt
      181 tgaacagctt gatattcata tccaatacct gaaccaccaa taattgcaat acgtttaatt
      241 ttctgattag actcaccaac aaaacggaca cttgggatat ttaatttaga tttaatatca
      301 gttgcgaagt cttccaatgt catttgatta tccacttctg ccataacgcc aagtccataa
      361 agggatgttt gttttatctc aataaaatca aataccggtg tttcatatgg atggtattgt
      421 ttaattaatt gctcagccct tgacttttga catgcatcta tcataaattc aattttaact
      481 tcatctacat attcaatttt atcaatttgt cctattgttg gattagcttc accaactggt
      541 ttgaattgcc ctcttccttc actttcaaag aaacaatatt cataattacc ttcttgtgct
      601 aatccatttt cactaagctt atctttaaat ggtccaacat tatccttagg tatatatgtt
      661 tgaactttat agtatacatc ttgttgatta tttattattg aaatgttctt caatcccatc
      721 gccttcgcca acatcatatt gacaccatga ggatttacat ctaaatttgt atgcatcgct
      781 attaaattaa tgtcatgttg aattagtttt ctaataatca aaccataacc attagctttt
      841 aatgatgtta cgcctttaaa gattagagga tgatgactaa taatagtatt atagcctttt
      901 tcgattgctt catttactac ttccaacgta cagtctaatg ctgttaaaac accagtaact
      961 tcaacatctt catcacctat taacaatcct acattatccc aagattcagc agttctaaat
     1021 ggcacatgat gatctaacaa tgccattaaa tcagctattt tcattactat aacacccttt
     1081 caattacaga aatttcttca ttaagttgag ctaaacgttg atgatgttgt tcagtattga
     1141 gtttcgattt aatatgataa agtgcttcta actctctttg ccattttttt ataaaatatt
     1201 catttttgtt gttcagcaat tttggtccga atttcaattc atcagatgat agctctatta
     1261 attgcgtaga atattctgct acaacaattt cataaatatg gcctttttct tccattatta
     1321 tttcatcaat tatttcataa ttcaattgtt gtaatgtttg tcttaaactt tcggtttgga
     1381 tattactttg taaaatcaac cttggatgtt gacttaactt atcttgccca tcttttaaaa
     1441 ttttagcaat aagtggtccg cccataccac aaattgtgat attatcgatt acgtcctcag
     1501 gatgaataac acttaagcca tcccctaaac gtacatcaat tctatctact aattgatttg
     1561 cagctacatt tttcacagca gcttgaaaag ggccttgaat aacttctcct gcaataccgc
     1621 aatcgcataa atggttttga attgcataga ttggcaaata agcatgatct gagccaatat
     1681 ccgcgattgt accttgtttt aaaaatcgac ttaccgtcgt taatcggtta tttaacgaaa
     1741 tcattttatt ctcctttatg aaaaaagagg ttgaacatta ttcaatgtac caacctcact
     1801 ctagttaaat cttaatctaa aaataaataa gcgcttattc atgtcttggt atcaatcatt
     1861 aaaattgata aaaattaatc cataaagtct ttcaaacgtt tactacgact tggatgtctt
     1921 aattttctaa gtgcttttgc ttcgatttgt cgaatacgtt cacgtgtaac accgaaaact
     1981 ttaccaactt cttcaagtgt tcttgttctg ccgtcatcaa gaccaaatct taatcgtaat
     2041 acattttctt ctctatcagt taatgtatca agcacatctt ctaattgctc ttttaataat
     2101 tcataagcag catgatctga aggactttgt gcttcctgat cctcaataaa gtctcctaaa
     2161 tgactatcat cttcttcacc aattggtgtt tctaatgaaa caggttcttg cgcaattttt
     2221 aaaatttcac gaactttttc tgctggtaaa tccatttctt caccaatttc ttctggtgct
     2281 ggatctcgac ctaagtcctg taataattga cgttgaacac gaattaattt attaattgtt
     2341 tctaccatat gcacagggat acgaatcgta cgtgcttggt cagcaattgc acgagtgatt
     2401 gcttgtctaa tccaccatgt tgcatatgtt gaaaacttaa atcctttgtt aaagtcaaat
     2461 ttttcaacag ctttaataag acccatatta ccttcttgta ttaaatcaag gaataacatg
     2521 cctcgaccta cgtatctttt agcaatactt acaactaaac gtaagttcgc ttctgcaagt
     2581 cttgattttg ctacttcatc accttgttca atacgtttgg ctaattcgat ttcttcttgt
     2641 gcacttaata agttaacacg cccaatttct ttaaggtaca tacgaactgg gtcatttatt
     2701 ttaacacctg gaggggcact aagatcactt ggattcagtt tctcgtcagt atctgaacta
     2761 tctttttcat taactagtga aatatcatta tcatttaatt gatcaaagaa atcatccatt
     2821 tgatcagagt cgatatcgaa attctgaagt ttttcagcaa tttcttcatg acttaaatga
     2881 ccctcttttt tacctttttc aattaattgc ttcttaacat cttctaatgt taatgtcgga
     2941 tcaattgttt gttttttaat tttaactgtg ttatcagaca tgaaacggcc tcccgatttt
     3001 aaatatgaac attcgaaatt tattcaatat tgctatttta aactaaattc ttaattaatt
     3061 catccatatt tttaatttta ttttacaaat tggaactaat cccaatattt atttttcaat
     3121 agtgttgttt aaatgcggta actatcattt acccttatac attttgacat aatcattatt
     3181 cgtattattc tttaaaatca catgctacat gcgttcctta ttcttagcaa caatttgctg
     3241 taaatagtgt ttttgtaatt ctacatcgcc aatccttgta gcttccctta atttatgatt
     3301 caatgactca attgtttctt gtccgttttc attaataaca ttgacataat catcaatttc
     3361 attttcatat ggttcatcat tcaaactata ttgttctaag ctaattagtg tttctctcaa
     3421 ctcatttgag ttaacatact gcacagcatc actgatatta tattgatcat tttccgcata
     3481 aaaatcatgt aagacttcga atacatattt aaaatgctga tttgtgaagt tatccttatc
     3541 aacactttca taataattta aaaatgtatc tttatctctc attaaatgtt ttaaaaatgc
     3601 acgctccgct ttttcttgac ggctcaaatt gtcaaattgt gccataccaa ttggctcagg
     3661 ttcaatgtaa ccgccatact catcttctgg ataataattg gctggtgctt gattgaattg
     3721 tatttcgtta gctaactgct caggactaac attgaaaaat ggcgcaacat catttatagc
     3781 cttttgttgt agaattgatg acttcataag tgaaatgtca tgactcagtt ctttcaaata
     3841 acgttcatat gaaaggtcat tatgtgcaat ttcatctttt aatatactca ctttataatg
     3901 tgcaaatgac tttttgtcat tttttacaaa agtagtaaat gcgtcgttgc catacttacc
     3961 aatgtattca tcaggatcca tgccagatgg caattgtata acaaatacat ttagcccttg
     4021 ctgtaacaaa tgttgacctg ttttaagtgt tgcttcacta cccgcaaaat ccccatcaaa
     4081 cattaatgtt atatttgatg ttaactttcg tataaaggta atatgttcat ctgacaactg
     4141 tgtacccatt gttgcaacaa cgtttttcaa gccagcagta tcagatttta taacatccat
     4201 aaaaccttct agtaatacaa tttcatctaa ttttctaatt gatttacgtg ctttatctaa
     4261 gttatataac aactttcttt tttgaaagat aggtgtttca ggactattta ggtattttgg
     4321 ttcttgaccg gtatatgttc gacctgaata tccaacaatt cttccttgcg catttttcaa
     4381 aggaaacata atacgatttc gaaatctatc gtaataactg aagttttctt cgttacgtga
     4441 taataaaccg gcttcatatg ctaattcaat atcgtaaccc tttttttgaa gaaaatcatg
     4501 acaaaaatgt gagctatcgg gtgcaaagcc aatgcctcgc tctttaataa gcgcatctgt
     4561 aaaaccacgt tcttgtaagt atgttaatgc ttgttcgcct tcgactgtct ttgttaaagc
     4621 gtaataataa aattcttgta ttaactcatg catttcaatc atttgtaaat catcagaagc
     4681 aatttgaaca tttgagttag attgtgttgc ctcaatatct acagcaacat taactctatc
     4741 acctaattct ttaaccgctt caacaaatga tatgtcttta atctcttgag taaattgaaa
     4801 aacattgcca ccttttttac aaccaaaaca atgacaaatt tgtttatctt cagaaactgt
     4861 aaatgaaggt gtcttttcat catgaaaagg acacaaacct atataattgc gtcctctctt
     4921 ttctaatttt acatattcac ttaccaagtc taaaatgtcg gttttatctt ttatttcatt
     4981 aatgatcgat tgatctattc gcaaattaaa tcacctatta agcattagtt atactattat
     5041 acctaatttt tgacaaaaat gaaatcattt cgatttattt tgttcaatat aatggattat
     5101 atcgtttgct gtttcttcga ttgctttttg agaaacatca atgacaggac atccaatttc
     5161 acttacgatt tcttcaaagt aattcaattc ttcttggatt cgagcttctg ttgcataacg
     5221 agctgtgtca cctagtccta attgttttag tcgctctttt ctaatgcgat ttaatttttc
     5281 ttcacttatt ttaagtgcga tacatttctt tggatcaata tcatataagc catctggcgg
     5341 tgtcacttct ggtacaatcg gtacattcat aactttgtaa ctcttatgcg ctaaatactg
     5401 ggataatggt gtctttgaag ttctcgaaat accaagtaaa acaatatcag ctttaggtaa
     5461 tcctttagga tctttaccat catcatattt aactgcaaac tctatcgcat caattttctt
     5521 gaaatatgca tcatctaatc tatgaacgat acctggctca ttataaggtt tttcttcaac
     5581 cgatgctgat aataaatcca ttaatggccc cataatatcg acggacttca attggaattc
     5641 tgctactttc tcactcatat attgcttcat ttcaggttta ataagtgtat aaactatgat
     5701 agcatttgta tctttagcaa cttgaatcac ttcatcaaca tcttcaaaag attcaatata
     5761 tggatatctt aataattcat ttttacattg cttaggatta aattgtgaaa tacctgccct
     5821 tgcaactaac tctgccgttt cacctataga atctgaggct acgataattt taattttttc
     5881 cattacttct cacctattct ttatataatg ctactaataa cttagctatt gttgttttgg
     5941 aaattcttcc aattacttca tacttttgat tatctttttt tcttacaatt ggaatcgaat
     6001 caatttcttt ttcaatcatt ctatctgctg cgtatatgac taattcgctt tcctctaaat
     6061 aagtgacatt aggcatacgt gtcatattta cactgatagg tactgtatga atatctgatc
     6121 caatcattga agctcttaat aaatcttttc ttgaacacac accaacaaaa tcgttatctt
     6181 cattaataat aaataatgta cttacatctt ctaaaaaaat tgtacaaata gcgtcataaa
     6241 ctgttgtatt ctctcttagc acaacaggtt gagacatata gtccttaacc tcaaattgtc
     6301 gaagtttttc attaaaaaat ttaccttttg atttacctga ataataatat ccaactcggg
     6361 gacgcgcttc taaaaaacct gacattgtta atatcgctaa atctggtctg agcgttgctc
     6421 ttgttaaatt caacttatct gctatttgtt caccagtaat gggtccttga gttttaacaa
     6481 tttcgatgat tcgttcttgt ctttgactga gttctatagg tcttcacccc ttttttattc
     6541 acttattatt ataccgatat ttctttcatg tcacaaacac attaatttta cttgccttta
     6601 aataatctat caattagaat aaacgtacag cgagttaagg atagtgtaag cttaacaata
     6661 agattggcgc aacgaatcat tttaaaataa aagcgagtga ctacactaat ttgggtggaa
     6721 ccgcgggtta aatacgtaac ttattttgta ttatatacaa tttaaaatat taactcgtcc
     6781 catggaagta aagcatattt actatgctgt tattttccat ggggcgtttt catgtatgag
     6841 gagaggtaat tatggcaaaa gatatggata caattgtttc attagcaaaa cacagaggtt
     6901 ttgtgttccc tggtagtgat atttacggtg gtttatcaaa cacatgggat tatggtcctt
     6961 taggtgttga attaaagaat aatgttaaaa aagcttggtg gcaaaaattc attacacaat
     7021 caccgtttaa cgttggtatc gatgctgcaa tcttaatgaa tccaaaagta tgggaagctt
     7081 caggacactt aaacaacttc aacgacccaa tgattgataa taaagatagt aaaattcgat
     7141 atcgcgctga taaattaatt gaagattata tgcaagatgt taaaggtgat gaaaacttca
     7201 ttgccgatgg tttaagtttt gaacaaatga aaaaaattat tgacgatgaa ggtattgttt
     7261 gtcctgtaag taaaactgct aactggactg aaattcgcca attcaattta atgtttaaaa
     7321 cattccaagg tgtaactgaa gattctacaa atgaaatttt cttacgtcct gaaacagctc
     7381 aaggtatttt tgtaaactat aaaaacgtgc aacgttcaat gcgtaaaaaa ttaccatttg
     7441 gtatcggaca aattggtaaa tcattccgta atgaaatcac tccaggtaac ttcattttca
     7501 gaacaagaga atttgaacaa atggaacttg aattcttctg taaacctggt gaagaaatcg
     7561 aatggcaaaa ttattggaaa acttttgcaa gtgactggtt aacaagctta aatatgagca
     7621 gtgaaaatat gcgtttacgt gatcatgatg aagatgaatt atctcattac tcaaatgcaa
     7681 caactgatat tgaatataaa ttcccatttg gttggggtga gttatggggt atcgcgagtc
     7741 gtacagactt cgacttacgt aaacatgctg aacactctgg tgaagatttc agataccatg
     7801 atccagaaac gaacgaaaaa tatattccat attgtatcga gccatcactt ggtgcagatc
     7861 gtgtaacatt agctttctta tgtgatgcat atgatgaaga aggcgttgaa ggtagtaaag
     7921 atgcacgtac agttttacac ttccatcctg cattagcacc atataaagca gcgattttac
     7981 ctttaagtaa gaaattatct ggcgaagcga ttaagatttt tgagcaatta agttctaaat
     8041 tctcaatcga tttcgatgaa tcacaatcta tcggtaagag ataccgtcgt caagatgaaa
     8101 tcggtacacc ttattgtgta acattcgact ttgattcatt agaagataat caagttacag
     8161 tacgtgacag agattcaatg gaacaagttc gtatgccaat ctcagagtta gaagctttct
     8221 taactgaaaa aacaaaattc taattaaaaa gactctccac catatgtggt ggctatataa
     8281 tttataaaaa gagatactgg ttagtaaaat tgccagtatc tcttttctat tcgaaaaagg
     8341 tattcaaata aacacatcta tgtggctact tgaatacctt ttattatttt atgttattgt
     8401 tccaatcttt ttaattggtt gattagtttc tgacttttaa aaaacatacc tgcatattca
     8461 cgatataaca ttaaaatgat ttctgacatt tcatcaataa tttcttgatg gatattcaat
     8521 gaattcattt tatctattgg taatttttgt aatacatcta ataagtatag tgttttattc
     8581 gataatataa ctgcatgtat atctttagaa gcctcttgcc ttgaaatcgc accgtcaaac
     8641 ttaaaactat aacctattaa atcagcttgt gtgtcattac cactcacagc acagcgatta
     8701 aacgatgcgg taaaaccaaa tcgtttcata cactttaaca taacaacgac tgacattaac
     8761 tgtgcagatg tacctgattc tattttttca agaacaaatt gtaataattg atagttatat
     8821 ggtgcaatgt caccttcatc cattgagcgc tcaatagttt cagctgccag agacgcataa
     8881 ctgcttacgt aaaggtccat ttgtaattta taatgttgac taataacatc tacagaattt
     8941 aacgttccca tacctcgcca ctgattgtaa ataaacaaac cataaacaaa caattgcgtt
     9001 tgcgcttgta aacccgtctt aactttttta gcacgccttg ccataagtgg tacttttgca
     9061 ccatgctcat ttaaaatcgt gataatttta tcagattcac cataatcaac tgctttgatg
     9121 ataatccctt tttggcgcat taacaattat cttcaccact tttaagatta atcttggtct
     9181 tcaacataac caatttggcg aataaagtta actttgtttc gccagtctct ttgaactttg
     9241 acccataatt ctaagtaaac tttagagcct agaagcattt ctatatcacg tctcgcacgt
     9301 tttcctactt cttttaactt tttaccgcct tttccaatga caattccttt ttgcgaatct
     9361 ctttcaacat atatagttgc ttcaatatga acacgatctt cgctttcttt aaccatacgg
     9421 tccacattaa caccaatcgc atgagggatt tcttcacttg taagatgaag gattttttca
     9481 cgaatgattt cacccactac aaattgttca ggatggtctg aaatttgatc atctggataa
     9541 tatttaggtc cttcgggtaa atacgtcttt aatacatcaa taaaatgatc tacatttagc
     9601 ccttctaatg ctgaaatagg tacaatctct gtaaagtcca tataactttg atattcttca
     9661 atctttggca ttaattcatc tggatgcact aaatctattt tatttaatac taaaaatact
     9721 ggtgtcttaa catttttcaa catttctata atatattcat cacctcgtcc aatttcctca
     9781 ttggcattaa ccataaacat gattgcatct atctcagata atgtattttt agcgactttc
     9841 atcatatagt cacctaattt gtgtttaggt ttatgaatac ctggcgtatc aatgaatata
     9901 atttgcgcat catctcttgt cattacacct tgaattttat ttctagttgt ttgagcttta
     9961 tcggacatga ttgctatttt atggccaatc actctattaa caaatgttga cttccctaca
    10021 tttggtctac ctataattga aacaaatcct gatttatgtt ctgtcattta ttctaaatcc
    10081 tttcctgaaa atccaaatgg tagtaactct gcgactgtca tcataaccat atctccttta
    10141 tgatttgtca tatacacagg catatcatca tcacataatt ccttcaaaac ttgacgacat
    10201 gcaccacaag gtgatgacgg tttatctgca tctacggtta cagttattga ttcaaaatca
    10261 cctggtctgt atccttgaga aattgccgat accaaactag ctcgttcagc acatatcgat
    10321 aatggataag aagcattttc tacattggta ccataaaaag ttctaccgtc tttcgttttt
    10381 aaataagccc ctactttaaa atgactgtat ggcgaatatg attcttgttg tgcctttcta
    10441 acttcttgaa aataatgagg ttgataactc atatatgcct ccctaaaata acgctataaa
    10501 atgtggtaaa aatactatta aacctataat aaatgctaat attgaaacta taagtacact
    10561 aaaagccgct atatctttag cgtatttagc taaatcatga tattcaacgg tcactaaatc
    10621 tacaacatat tcaatagcag tgtttaaagc ttcaacagtt agaactaatg caatagcaat
    10681 gagtataaat atccactcaa tccgattaat atttagtacg agaccaaaga caatagcaac
    10741 aatcattgca aacacatgta aaagaaattt atagtctttt tgaattaaga ttttcagccc
    10801 atcaagtgca tatttaaacc ttttcataat tagtctcgtg ttaatccata tgcgtttaat
    10861 attgtatctt gtcgaccaaa catttccttt tcatccgctt cagtcatatg atcataacct
    10921 aatagatgca aaaatccatg taatgctaaa aatcctaatt ctcgttcaaa agaatgtccg
    10981 taattgtttg cttgttcttg cgctacatcc gtacagataa ttatatcccc taaaacacgt
    11041 ggtatatcaa aaccactaaa atcaatatct ggctcatctt cttctaaagc aaatgagatt
    11101 acatctgtaa ctttatcttt atctctatat gttcgattaa tttcttgtat ttcttgttta
    11161 tctacaaatg taacagaaag ctcagcatcg tcttctatat gctcttcttt tttagcaaat
    11221 tctaataaat ctttaatttg tttataccaa gcatctttaa ctaagcctgt gtgatcgcta
    11281 aaatctatcg taaacattta attctctcct tcataatgtt caatgatctt acttaccaat
    11341 ggatgtctta ctacatcgct ctgatctaat ttcaatatac ttataccttt aacgttgtgt
    11401 aacctactga ccgcttcctt aagtccactt ttaacacctt taggtaaatc gatttgagtt
    11461 tggtcaccag taactaccat ttttgagcca aatcctagtc ttgttaaaaa cattttcatt
    11521 tgcgcatgtg tcgtattctg cgcctcatca agaattacaa atgcatcttc taatgttcgt
    11581 ccgcgcatat atgcaagtgg cgctatttcg ataatgcctc tttcaataaa tcgctctgtt
    11641 tgttcacacc caagaacagt atatagacca tcatataaag gtcttaaata tggatccacc
    11701 ttttctttca aatctcctgg taaaaatcca agtgactctc ctgcttcaac agcaggtctt
    11761 gttaatacaa tgcgtttaac agcaccttta cggagttgct ttgctgcata aactacagct
    11821 aagaatgtct tacctgtacc agcaggacct ataccaaata ctaaatcatt atttttcatg
    11881 gcattaacat atatacgttg ccccatcgtt ttcgcacgaa tcgtcttacc aaatgcatct
    11941 ttagttatct cttcatcata taaatctaac agatgttgaa ttgtgttatt atgcgccatt
    12001 ttaatagctg cttcaacatc tttaattgta atattattac ctaaatcaat aaccttcagc
    12061 aaattgatta atactgattc cgctttttct acgttttcta tttttgtacc tttaacggca
    12121 acttcttgtc ctcttgcatg gatgacaaca tcgaaactct cttcaattgc ttttaaatgt
    12181 tcatcattat ttccaattaa agcttgagat tggttcatat cgtctatttg tataattcca
    12241 ggcatacacg cgctcctttt catatatcat ttatattcat tctatgatgc tttaatatct
    12301 acttaattat atcatgcaat tgattgattt aaacatcttt gcattgactt actcattttt
    12361 ctttaattta ataaaatcat ttatactttt caccttgtca taaaagcaga ttcaatttta
    12421 gactaatcaa ttttttgttg taatttaaat aactcatatt tctcgaaaga ttctctttgt
    12481 ttttcaattt ttcttaacgt aaaaaaacaa gcaactacca ttacgatagt gcttgttttc
    12541 aaaaaatttt ataattgttt tggtttagct aaaatttctg accatatcat accattgatt
    12601 acttcatctt tatcaaattg gaacgctgat ttagtcaaat ctttttcgac attttgagaa
    12661 ttaagcaact gctttaattt caaacgtttt gtacgttcag ataagtattt atcctcaata
    12721 atatctctag ctcgtttttc cattttagca atttgctttt ctttttctct atcaatgtca
    12781 gtacgaatcg ttttaatatc atctctaagt gatttttcta attgacgtct aatttcgtca
    12841 gaattatctt gtctagaagg tctatgtttc tgttccaccg gttctgcttt aggcactgta
    12901 attggtttct ctgtcatcgg ttttgtttct tgttgttttt taggtgccat aggttctaca
    12961 acaggtttcg atttaggctc ttcctttgga agttcatcga ataaaggtgg taacgtatca
    13021 tcatattttc gtttcgatga tttcttttct tcttcattta attcttcact tatttcttta
    13081 aacgttcgct caatttcttc aaaaaagcca cctttttttg gttcattatc ggtagatgtt
    13141 ttttgaggtg gcttttgatt ttgtctatct ttatgactat tttcgcgcat agtagtaatg
    13201 atagaaatga tcactgatat gacaaaaatt agaataccga cactcattta atcacctctc
    13261 gacttaatgt tcaggtaact catcatcact ttgatcagtt cgtttattaa ttgcatttct
    13321 catgcctgta tcagcttcga tatttttcaa attataataa tctttaacac tgatattacc
    13381 tgaacgtaat gcttcagcca tagctaatgg tacttcagat tcggcttcaa ctactttagc
    13441 atgcatttct tgtacacgcg ctttcatttc ttgctcagtt gctacagcca tagctctacg
    13501 ttcttcagct tttgcttgtg caatattttt gtctgctaat gcttgttcag tttgtaagtc
    13561 tgcaccaata tttttactaa tatcaacgtc agcaatatca attgataaaa tttcaaatgc
    13621 agtacctgaa tctaaacctt tgcttaatac tgttttagaa atattatctg ggttttcaag
    13681 tacttctgta tgatgcttac tagaaccaat tgttgaaacg ataccttcac caacacgtgc
    13741 aatgattgtt tcttcaccag caccaccaac aagtcgagca atattagctc taactgtgat
    13801 acgagctttg gctttcactt caataccgtt cattgctaca cctgcgataa atggtgtttc
    13861 aatgacttta ggattaacag acatttgaac cgcttctaac acgtcacgtc ctgcaaggtc
    13921 aattgcagca gcacgttcga aaggaaggtc tatgtcagca cgttgtgcag caatattagc
    13981 gtcaacaact ctgtccacat ttcctcctgc tagataatgc gattctaatt ggtttgttgt
    14041 taatgctagt cctgctttat gcgctttaat taatggcgct ataacttttc ttggagatac
    14101 acgacgtaaa cgcataccaa ccaatgtacc tataccaaca tgaacgccag ctgctaacgc
    14161 tgaaatccat aaaccaatcg gtacaaatga gaataaaata agtaatgcaa ctactataat
    14221 aactgctatt acgataaaac ttaaactaaa catggtatcg ctccttttaa ttaatctact
    14281 tccctcacaa ctactcttgt tccttcaact tcaaggattt ttaccgtttt attgcgtaaa
    14341 ataaagttgc catctgaaac agcatcaata cgttcatttt cacaaaaaat aatccctgct
    14401 ggtcgaagat ctgtaactgt ttgagcagtc tttcctacga ggtgcgagcg gttatcatga
    14461 gaattgtaac ctgactcaga attagttgaa tcttttaaga taactttatc caaaaacgga
    14521 atctttctgt tgaaaatctt cactaatatc acccattcta caatcgttaa aatcaaggca
    14581 acgataacat tcgcaagcat aaatagcaaa ttatcaccga gcgtcgttat gcttatagtt
    14641 atcagtatca tgccaataat accaattact gcaccaacta caaataattc aattacaact
    14701 aatataacgc caattgagaa tattaagata gaatgcatat tgacatttcc ttggattaga
    14761 aatcccaaaa ataaaataag taatgataat gtggcgataa taccagctgc attgattttt
    14821 ttagagtaaa gttgatatat aaatcctaag aatgtcaaac aggttaatat taacgtaaaa
    14881 ataggttgaa caattatatt actgacttgt tcaacccaag tatctccagc cgttgattcc
    14941 aagatagttg tcatttgtaa aaaattatta taactcacaa tttcacctcg ccctcacatt
    15001 aattatacat gaaattgaat atactttata gttataagaa cctatagaat atcaatttga
    15061 ttatttataa actatataac tttttttaat taaaaattga c
//
LOCUS       DAHHRR010000057        15011 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.057, whole genome shotgun sequence.
ACCESSION   DAHHRR010000057 DAHHRR010000000
VERSION     DAHHRR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15011
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.057"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..1498)
                     /locus_tag="NBI42_001532"
     CDS             complement(<1..1498)
                     /locus_tag="NBI42_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1777952.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDV
                     KRNDVTVIMLNDLYQIDGHDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSIIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSTERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYYLEPNELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLINENNDLKEKITIFDINKVTELLTN"
     gene            2107..2916
                     /locus_tag="NBI42_001533"
     CDS             2107..2916
                     /locus_tag="NBI42_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1777953.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNYNNYNNGYSYNNYSR
                     YNNYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSG
                     YTSGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQT
                     TQGAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQASSYNFIH"
     gene            complement(2913..3170)
                     /locus_tag="NBI42_001534"
     CDS             complement(2913..3170)
                     /locus_tag="NBI42_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777954.1"
                     /translation="MTKLIENLKASIFASTLTKNLSMYSRFDAPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKASKVIETLLATFITN"
     gene            complement(3362..4744)
                     /gene="nhaC"
                     /locus_tag="NBI42_001535"
     CDS             complement(3362..4744)
                     /gene="nhaC"
                     /locus_tag="NBI42_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCD1777955.1"
                     /translation="MMARQPSFLESISTIVVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     AWIAKRVGLTWQDLEKGIADRLNTAMPAIFIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAITSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGCQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAILISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNDSMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVGEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKS"
     gene            complement(4840..5922)
                     /locus_tag="NBI42_001536"
     CDS             complement(4840..5922)
                     /locus_tag="NBI42_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HCD1777956.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVDKGHEVKLYCRNQSINKFQNAIDK
                     GGFDFNNEGEERFVEFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTNDQL
                     IFFNMAAAMGAIRFMNVLEDRHIDTKPQLAEANTLTYGTRVDFENAKVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDYLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            6173..6595
                     /locus_tag="NBI42_001537"
     CDS             6173..6595
                     /locus_tag="NBI42_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCD1777957.1"
                     /translation="MTTEMNDVTNQQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYFNYTISYIIWSIVLVIITFIGVFLIATDIDFIVIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYVIPLTIRFI"
     gene            6831..7331
                     /locus_tag="NBI42_001538"
     CDS             6831..7331
                     /locus_tag="NBI42_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1777958.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            7691..8644
                     /locus_tag="NBI42_001539"
     CDS             7691..8644
                     /locus_tag="NBI42_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HCD1777959.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     AIALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(8736..9860)
                     /locus_tag="NBI42_001540"
     CDS             complement(8736..9860)
                     /locus_tag="NBI42_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HCD1777960.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRPLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNESDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLVVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(10171..10947)
                     /locus_tag="NBI42_001541"
     CDS             complement(10171..10947)
                     /locus_tag="NBI42_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCD1777961.1"
                     /translation="MKKKFKIRISTLLLMIILVIFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVSSGKSYAEKEQWISPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            complement(10968..11201)
                     /locus_tag="NBI42_001542"
     CDS             complement(10968..11201)
                     /locus_tag="NBI42_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777962.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            complement(11496..11969)
                     /locus_tag="NBI42_001543"
     CDS             complement(11496..11969)
                     /locus_tag="NBI42_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HCD1777963.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKVLDDAKILDALNGAVRGRQVIINKFAVELNKDIYTGLLTNMASMVFLLGELNVSDL
                     SEFLNKVNKGLHVANQASPNAKTSVRSLLGVLKDDDMNRSLTYMLNMLKGMSRED"
     gene            complement(11969..14923)
                     /gene="fdhF"
                     /locus_tag="NBI42_001544"
     CDS             complement(11969..14923)
                     /gene="fdhF"
                     /locus_tag="NBI42_001544"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCD1777964.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTSIKEKYGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIEEWVEDFDEYYK
                     SLETFTMAFAEEATGIPEAELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKVGKDNHEMVEGVHDGEIHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATFADVVLPASPSLEKDGTFTNTERRIQRLYQALKPLGDSKPDWKIFQAIANKLG
                     FDWNYKHPSEIMDEIARLTPLYAGVSYERLEGFNSLQWPVHPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEVYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRHPQYSVQVQKKWER
                     PDYVFPGNQVDK"
BASE COUNT         4920 a         2815 c         2078 g         5198 t
ORIGIN      
        1 aatttgtcaa aagttcagta accttattaa tatcaaaaat tgtgattttt tcttttaagt
       61 cattattctc attaatcaaa tctacatcac atataatttt agaacgcgca atagacacct
      121 gttctatttg tcttagtggt aagataaaat agtctattag atgatcaatt tctttatcat
      181 agcgcgtatt caactcattt ggttccagat aatatattaa ttcaaatact cctatataat
      241 tatctattaa acgtttcagt ttttgaattg cagtagtatt taagtcgtta attaaaaatg
      301 caatcttaca tctattactc tcaaaagtca tattaaaaat tgtagctact aacttttcta
      361 tcttgcctct ttcagtactc gttaacaatt ccttatattc atgtttaata cgaaatacta
      421 catttacatt gtaattcact atcttttgaa gtaataaagt gatttgttca atcgatagct
      481 cattgcttgt gtcgattgta acaattatat gcaagttttc aggattaaca cctaatcttt
      541 gaatgatttg tttaatagta taataatcca tccaataaaa attacttcct ttaatataaa
      601 tgtttttagg ttgatacatt tgactccttt ttgtaggctc aaaaggtata tcaatctcgc
      661 gcatacttga agaactttga ttagtatcat caaataattc aattatattt ttatcaattt
      721 caactaattg ggaatggtta attgaatgag atgttggact atatcttttt ctaattaatt
      781 taggagtgtt accatacgtt tctttaaaga ggtgtataaa acgagaataa tgattaaaac
      841 catgactact tgcgatttct tcaataggct tcttagaagt taaaatatca attaagcaat
      901 gctccagtct aatatgattt aaatattgaa taaaattact ataaggtgtc gctttaaaca
      961 tgtcacttaa agctttattt gtaatactaa cttgattaat gacatctttc ctatttatct
     1021 ttttatggtg gttgtttgtt aaataatcgt gcacttcttc ggctactaaa tgacgactac
     1081 catcgtatgt atttaatgac attaattcaa cacacatgta attaataatc ttatcattag
     1141 cattataaga ctgttgttta attatgctgt agattaaata cttaatcaag attctaaaag
     1201 tgctagcaac ctcatttgtt aatatcttgc cacgcatcat atagtttgca ttcataaact
     1261 tcaaaaatgt ctttgcactt acttcaacaa cacaacatac actatcatca tgaccatcaa
     1321 tttgatacaa atcattcaac ataataacgg tgacatcatt tctttttaca tcatattgtt
     1381 gtagattgat tgaaactaca cctgtacctt gtagccaata tataattttt aagttcgctt
     1441 ccttcacact actcattttc atacttttat aaatatgtag tgaataatta tctgtcatct
     1501 tacatcctca catatacaaa tatattgatt tactcctgtt tgtctttctc aaatcaattg
     1561 tcaacatcta atattcacta ccaattatat cgccacatgt tttttagcag taacaaaaca
     1621 taaatcgatt atatcaaata atacttttat taaaacattt tttatgctaa attaggatat
     1681 tttatccatt aaatatacat tttcatccaa ataccaaagc tttcataatc atagaaactt
     1741 gatcgttcgt gtcgaatttt tatgcatttt ctgattaaaa gtcctatatt atgcacaaaa
     1801 tatacattta gaattttcag taatattaca aatttgtaac agacttattt tcgaaacatt
     1861 cagttttaac aaagaatgcc gtagttataa gttttgcaaa atgcaaattt ctatactttt
     1921 aaaattctat tgtgtcaaga tattagcaaa aaattacaaa acgttaacaa agcacacaag
     1981 gacgctaatt tctgttatag tctttcttgt cgtttaatta cgagagataa cttataacat
     2041 taattttgtc acacggacat tacgaaaata ttttagattt aaaattttta ggaggatatt
     2101 tttaaaatga agaaaatcgc tacagctact atcgcaactg caggattcgc tacaatcgca
     2161 attgcatcag gaaatcaagc tcatgcttct gagcaagata actacggtta taatccaaat
     2221 gacccaacat catatagcta tacttacact attgatgcac aaggtaacta ccattacaca
     2281 tggaaaggta actggcatcc aagtcaatta aaccaagata atggctacta cagctattac
     2341 tactataatg gctacaataa ctataactac aacaattaca acaacggtta tagctacaat
     2401 aactacagcc gttacaacaa ctactcaaat aataatcaat catataacta caataactat
     2461 aatagttaca acacaaacag ctaccgtact ggtggtttag gtgcaagcta cagcacttca
     2521 agcaacaatg ttcaagtaac tacaactatg gctccatcat caaatggccg ttcaatctca
     2581 agtggttata cttcaggacg taacttatac acttctggtc aatgtacata ctacgtattt
     2641 gatcgtgtag gtggtaaaat cggttcaact tggggcaatg caagtaactg ggctaacgca
     2701 gctgcaagag ctggttacac agtgaacaat acaccaaaag ctggtgcaat tatgcaaaca
     2761 actcaaggtg catacggtca cgttgcatac gttgaaagtg ttaacagtaa tggttcagta
     2821 agagtttcag aaatgaacta tggttatggc ccaggtgttg taacttcacg tacaatctca
     2881 gctagccaag cttcatcata taacttcatt cactaatttg tgatgaaagt agccaacaag
     2941 gtttcaataa ccttgctggc ttttttaatt tctatgaaaa aaatcgaatt ggtaaaaatc
     3001 tcaaaatttc gattttcacc aatccgattc actctgtaat aaagaataaa caacatacag
     3061 ccattgacac acccaaattt aggcgcgtca aatcggctat acattgataa atttttagtt
     3121 aaagtacttg caaatatact tgccttcaaa ttctcaatta atttagtcat tacaaatcat
     3181 gacataaaac ttacattaca tatattaaca tgttccgcta caaaacatat tcagtatatg
     3241 tatagaggat ttttattcta agttaaaacc ttttcaaatt ataaccgcta caattataaa
     3301 tgattaaagt gtttcaacat attatatata ttccacgctc acattaagtt aaacgtgaat
     3361 tctatgactt tttaatacct atccctgtaa aaccatagat aattgctata attgcgcata
     3421 aataacatgg tactgtccat atgaaaaatt ctccaacaga gacatgaagt tgattcgtat
     3481 agtaaatacc tgatgttccc catggtatta aaggtaagac catcgtactt gagtcttcaa
     3541 gtgttcttga taaaacggaa cgagagactt ggtatttttc gaacaaatct ttcattaaca
     3601 caccgaccat aataattaca atcgaagcaa cacctgcagc gaacacaagc gcaatacaac
     3661 aaataacagt aatacatatt aaacttccta cagaatggat tcctttagaa atagtagtta
     3721 ataagacttc taaacatcct gctttttcta caatacctgc aaatgcatat ccgcaaaaga
     3781 tcgttactaa tatttgggtc atactcatca taccaccctg ttctaacaag cttttcacac
     3841 tggatgaaat atgagactga tgtatcattg agtcgttaaa accactaaat gttgctttga
     3901 aaccatctgt cattttgaaa tgatgattaa aagttcccac tataatggca gaaaagcttg
     3961 atattaggat cgctggcact gtagccattt taaatagcaa acaaacaatg atcacaatta
     4021 agggaaccca gacccatatg ttaatttgat aaatctgtgc aagctctgat aacaaagttt
     4081 gaatctgttt atcatttgaa tgccctttaa attgacatcc agcaataaac catactaata
     4141 aaccgataat tgacgcaggt attgttgtcc acatcattga atgaatgtgt ttaaatatat
     4201 tcactttagt gacaagggct gctaaattag tcgtgtcaga taatggtgac attttatctc
     4261 caaatacagc gcctgctata atagcacccg ctgccatccc tggaggaatc cccaattgat
     4321 taccaataga aataagtgca atccctgcag ttgatgcaga gccccatgct gtaccagttg
     4381 ctacagaggt aattgcactt ataaaaaatg cagatattaa aaaatagctt ggattcaata
     4441 aatctaagcc ataataaatt aaagcaggaa ctgttccaga aaacatccaa cttcctacaa
     4501 ttataccgac tgctaaaata atgaaaatgg caggcattgc agtattcagt cgatccgcaa
     4561 tgcctttttc caaatcttgc catgtcaatc ctacacgttt agcaatccat gcggcataag
     4621 ctgaagcaat gattaataac acttgaattg gaatgtcaaa aaatacaaat cccgttacta
     4681 caacaataac cataactact attgttgaaa ttgattctaa aaagcttggt tgtcttgcca
     4741 tcatatatcg tcccctttgt ttattgatgc aaaaccactt ttcttcaatt tattttccat
     4801 tagtacattt cgatttgctt tttatattta aagtaacttt tattttaaat atttttctaa
     4861 atcaagctta tctaaaccaa tttcttcaac tgttaagcct tcctcaaaga agtctctctc
     4921 taaaatggtt gatgcaatta caattactgc atctatattt ggtgtcggta catcaataac
     4981 acgacctaag cttgaccata atactaaacc atatgcaata tcctcagtta aatatctgct
     5041 ttctacatga tttggtcctg ggatttgtga aaatactggg cttgtattaa ataatcggtt
     5101 caatggttcg tcttctttat cacgttctaa ataaccacgc tcaatacgtg attctttcgc
     5161 agttgataat tcaaatccta atctacgccc taaattcaat ctttctaact caattgcatg
     5221 taataatctt accgtatgtt ttgtaatacc ttctttatat aatgcaaact cacccgcata
     5281 atcaatgcgc cccacattta ataaagttgg tcctggatgt acttcaggat taccattttc
     5341 aagatttgtc ttaattaaac tttcttcttt aactaaatag tcataaatac ttgaaacttt
     5401 atcataacaa tcatttaggc aacttctgtc atacgttgaa aagaagatac gacgtacgtt
     5461 taaagataaa tcaacttttg cgttctcaaa gtcaacacgt gtgccatacg ttaatgtatt
     5521 agcttcagcc aattgaggtt tagtatcaat gtgtctgtct tccaaaacgt tcataaaacg
     5581 aattgctccc attgctgcag ccatgttgaa gaatatcaac tgatcattcg tcacatgctc
     5641 tgccattaca tcagcataat attctatgta tgaagatggg atgataactt gcacaatctc
     5701 agcatctttt agcacatact ccatatcatc gctaatatca gtgaattcta cgaaacgttc
     5761 ttcgccttca ttattaaaat cgaatccacc tttatcgatt gcgttttgaa atttatttat
     5821 tgattgatta cgacaatata atttaacttc atgacctttg tctaccatgt ccactgctgc
     5881 cgtaaccgca ccatttcctg atcctacaat tgctattttc ataaacaacc ctctttctat
     5941 ctttctttgt ttaaaaataa aaacacatta tacgtaagac aatataaaaa tgatgcgaat
     6001 tcatcacgca tcattataaa tgttaagtat atataacctt aaattaacct atttaaacgc
     6061 caacgtctac cacttatttt taaatttaat aattgtttaa taaacatgtt tattttgatt
     6121 atattttgtt atagttaaag ataatattac aattttcaaa ggagtgtcta atatgacaac
     6181 tgaaatgaat gacgtaacaa atcaacaacc gaatcgcaaa tccaatgatg aaaacattat
     6241 ggcgatgctt atctatttat taagtttatt cacatctatt attggtccac tgattatatg
     6301 gttattaaaa aaagatgaat cgaaactagt tgatcgtgct ggaaaaaatt attttaacta
     6361 tacgatttct tatattattt ggtctatcgt tttagttatt ataactttta taggtgtttt
     6421 cctaattgct accgatattg atttcattgt cattataggt ttcatcatta cgtttattgg
     6481 tattttatcc atatttgctt tctcaatatt atcttttgtt ttcacaatta ttgcgtgtgt
     6541 caaatattat aacggccaag aatatgtaat acctttaaca atccgattta tttaaacata
     6601 ttacaaataa atattttcta ttaattacct gttaatctca ttgtaacttt ggctattttt
     6661 tattacaaaa ttagtcaaag tttttttatt cttttacaaa acaataacat tccccacaaa
     6721 tgagattaat tttgttaaag taactattgt catcaattga gatgtgagaa taaaacagtc
     6781 gctacgtttt atttataaaa aaagatatag aactttggag gactttattc atgaaaaaat
     6841 tagtaacagc aactacgtta acagcaggaa tcggtacagc attagtaggt caagcacatc
     6901 atgcagatgc tgctgaaaat tatacaaatt acaacaacta taactacaac acgactcaaa
     6961 ctacaacgac tacaacaact acgacaacta catcatcaat ttcacattct ggtaacttat
     7021 acactgcagg acaatgtact tggtatgtat atgataaagt aggcggagaa atcggttcta
     7081 cttggggaaa tgctaataat tgggctgctg ctgcacaagg tgctggattc acagtaaacc
     7141 atacaccttc taaaggcgct atcctacaat cttctgaagg accatttggt cacgttgcat
     7201 atgtagaaag tgtaaacagt gatggttcag ttacaatttc agaaatgaat tatagtggcg
     7261 gacctttctc agtaagttca agaactattt ctgcaagtga agcaggtaac tataactaca
     7321 tccatattta atataaacac gatgatacat attgtcttga aacttaattc tagtacatat
     7381 gtcatcaaaa actttgagct ttcctttata ggagaagctc tttttttgct atttttcgtt
     7441 gccacctact tatttatatt tcctcccata ttcataatat taattttttc aatactatta
     7501 actttaaaac tacgttatct aatttaattg atccagtttt tccacactac aatattcata
     7561 gttctacagt gcctaagtta tatcacctcc atcaaaagta cgcgcttaca tcattgatac
     7621 acaagacatc atgacatagt tgtatactgt ctaatcttct actatactaa gataaaagga
     7681 gtgatctttt atggaaaaag tttatgttgc tggtgcaata ccagaagtag gtttaaaact
     7741 tttacaagaa cattttgaag ttgaaatgta tgaaggtaaa ggattagtcg ataaagacac
     7801 tttaattaaa ggtgttaaaa acgcgattgc cttaattagt ttattatcta caaacgttga
     7861 taaagatgtt atcgatgctg gtaaagactt aaaaattatt gccaactatg gcgctggttt
     7921 taataatatt gatatcgagt atgcccgaga aaaaagtata gatgttacaa acacacctaa
     7981 agcatcaaca aacgcgactg ctgatttaac aattggatta gtacttgcag tagcgcgtcg
     8041 tattgttgaa ggcgaccaat tatcacgtac cactggattt gacggatggg cacctttatt
     8101 tttcagaggt agagaagtat ctgggaaaac aatcggcatt atcggtttag gtgaaattgg
     8161 tagtgcagta gctcgtcgtg caagagcatt tgacatggat gtactataca caggacctaa
     8221 tcgcaaagaa gaaaaagaac gagaaatcgg tgcaaaatat gtagatttag atacactatt
     8281 aaagaatgca gattttatca ctatcaacgc tgcttataat cctaaaatgc atcatttaat
     8341 tgatacagaa caatttaaaa tgatgaaatc tacggcgtat ttaatcaatg cctctcgtgg
     8401 tccaatcgtg cacgaacaag cactagtgca agcattgaaa gataatgaaa ttgaaggtgc
     8461 tgcacttgat gtatatgaat ttgaaccaga tattaccgat gacttaaaat cacttaataa
     8521 tgtagtatta acaccgcata ttggtaatgc tacatttgaa gcacgtgaca tgatgtctaa
     8581 aattgttgct aatgctgcaa ttagtgccgt tcaaggtgag aaaccacaat ttgtcgttaa
     8641 ctaataattg aatttattat aatgacatta taaaaacaag aacccccata tgttgagctc
     8701 tcgtttcaac atacgggggt tttcatattg ttgtattatt tttctttcga tttatataag
     8761 aatttagttt gtccagctgc taatgcattt ggcatcattt tcataatacg atttctaact
     8821 gcaacaacta aacgactacg atattgggca attttaccga tttttctaga acgcttaatt
     8881 acttttgcag tatgtttgac acgtatttta tcataacgct gtaatgcttt ttcaaagtcg
     8941 tatgcgttaa aacaatttac taatacgata gcatcttcca ttgcttgtcc agcaccttgc
     9001 cccatattag gcgttgtcgc atgtgctgca tctcctagta aaatagtacg accataaaca
     9061 aaagatttga gtggtttcaa atcataaata ttatgcaata agatacctgt ttcactttgt
     9121 ttgtctaaga tctctctaac ttcatttgga tagtgattaa agtatgcttg caaatgaggt
     9181 ttaccaaacg aactatattt atgattgttt tccttcgagt taattgtaat gaaccaatat
     9241 gcttgattat ttaataacgg aacaatacct actcttcctt ttcttcccca gtattctttt
     9301 gcacaatccg gatgctttaa atcaatatca tcaattaaac ctctaaagca tgtataccct
     9361 tgatataata ctttactgtc agcatttaca gattgtctca ctttagaatg aattccatca
     9421 gcaccaatac ataaatcaaa tgcttcactc tcttgttccg cgaaatgtat ggtaacttta
     9481 tctgactcat tatctatatg cgtgacttca tgatttgtaa atattgcatc atcttttaca
     9541 taagatttaa taatgtcaat taatgtttgg cgtggtaacg tcacattcaa tgtattactt
     9601 tttaatttaa cagtagttaa cgggcgatct ttgtcatcta acactgtcat tgtagataag
     9661 atttgcccag catttttaat acctttagct aagtcatgat tacctagttt tttaagcaca
     9721 ttatctccga taccaatccc agcgccaatt tctttaactg actcattttt ttcaaagact
     9781 ttaatagtat gaccttgttc ttgtaataat gcagcagctg ttaatccacc gatgcctgca
     9841 cctataattg ctatcttcat attgtcacct ctatattttt cattcttttc tattttatca
     9901 catgcactat tgtttaaaaa tgactcttgg aactaacgtt tcatttttaa agacttaagt
     9961 gtattatctg caccattgta tattacatgc acatttagac agcaatattc atttacataa
    10021 tgtacatttg caacaatgat gtgggatata gcatatttga tgctatatag cgttttttgt
    10081 taggttaaaa tccatttaac taacacactc agaatgcgct aaaggagctc gaaacaataa
    10141 attgttccag cccctttaag caccgatgtc ttatttataa tatgtttgtc taatatcatc
    10201 tttctttata ccaaactgct tataggattt atccattaat ttggcaattt tatctgccca
    10261 gcgaatgtcg ctcgcatatt gatgttgcgc aggattttct ggattccatc gcatttgata
    10321 taaattcagt tgattgtttt caaaatattc attacgaatg aactttgcac caccaataat
    10381 cgccttatct ggtgatatcc attgttcctt ttcagcataa cttttcccac tactaacagc
    10441 actactatcg aatgctccta taccaaaaaa gttgtaatag cgtttttttc catctttaat
    10501 gccttttgct aattctgatt tgccgttacc tgtttctact aatgcatggc taacaagata
    10561 aatgacatta acctcatatt tttcttgagc ttctagaaaa actttccctc gattttctaa
    10621 aatcccctta ccttttagca attgattaac ttcagattcc gacattggca ctttttctgt
    10681 gatatccata tacttcatat cattgtcttt atgtgaaata gtcattgctt tagcaatttc
    10741 attattattt gcttcaacaa atttaccatc ctcttctttt gtctgtacaa tacctttccc
    10801 ttgttgcatt gaaacagcct catcaaaaga gtaattcaca tcatttttaa acaatttagt
    10861 ttcattcacg atgagtaata cagcaaatat aactaaaatg atcattaata gtagtgttga
    10921 tatgcgtatc ttgaatttct tcttcatata gacaaaacct cattttctta cttagacaat
    10981 aaaaaatatg ccacaacaat cgctaatatt acgattaaaa aagaagcgtt aacgattact
    11041 ttcatcgttg ttctatctct gaacatcata ttaaagacaa ctagactaat tgataatgaa
    11101 acagcaaaaa aagtaatagc taacactaat ttcatcataa atagacagac taaacctatg
    11161 actaataatg tattagaaat tacagctgac gtttttaaca ttctcgaatt aatatgcact
    11221 cacccttttt atttaaataa cttgcataat cataataata catgatgttt catagccctg
    11281 tcgatggttg attatcaata gcacatgatt ttctcgattc tcaaaaatat tgtttaaagt
    11341 ttgatgaaga ctcggataca aataatattt caatataact taaaacagtg ctcctacacc
    11401 gacatgcagg aacactgtta tattcaaaga caaaaaaatc ggaaagatga aaagaaaaaa
    11461 acttttgcat caatccgatt tattatagac ccatattaat cttctcgaga catacctttt
    11521 agcatattaa gcatatatgt taaactacgg ttcatgtcgt catcttttaa aacgcctaat
    11581 agacttctta cagatgtctt cgcattagga ctcgcttgat tggcaacgtg taaacctttg
    11641 ttaactttat taaggaactc acttaaatct gatacattca attcgcctaa taaaaatacc
    11701 attgaagcca tatttgtgag caaaccagta tagatatctt tgttgagctc aacagcaaat
    11761 ttattaataa tcacttgacg tcctctaact gcgccattta aagcatctaa tattttagca
    11821 tcatctaaca ctttaataag tttgattgct tttaatatac tatctttatt cgctgcaatt
    11881 gcctctgtaa cttcatttaa actttctaac ttaatttgtt cttctgattt ttctaagcgt
    11941 ctaattttag aagatattct ctcagccatt atttatccac ctgatttcct gggaaaacat
    12001 aatctggacg ttcccatttt ttctgtactt gaacactgta ctgcggatga cgttttttat
    12061 tgacacggaa attattaggg ttcaacggtg acttaccccg tttcgtaatt acttccaaac
    12121 gacagcttgt acgtttataa gatggtgtat ccgtgtattg atcaacatca ctattagtta
    12181 ataagttaat tgcgcctaga tctccatttt ccatcgcatc attatttaat ggaatataga
    12241 tttctttacc tttaacacga tctgtcacgt gaacttgtaa taccgcttct cctgtttcag
    12301 aaatcagctt aacttctgca ccttcatgaa tgcctctatc ttcagcaagc tctggagaaa
    12361 tttcaacaaa tgcacgtggc actttgtatt taatcatcgg tgtttgataa gtcatattac
    12421 cttcatggaa gtgctctaac aatcgaccat tgtttacatg aatatcataa acttcatctt
    12481 gcttaaagta attatcaaat gataatggga ataattttgc tttaccatta tcgaaattaa
    12541 atccttctaa ataaagtatt ggttcatctg taccatctgg atgcacaggc cattgtaaac
    12601 tattaaatcc ttctaaacgt tcgtagctta cgccagcata taatggtgtt agtctagcaa
    12661 tttcatccat aatttcgctt ggatgtttgt aattccaatc gaaacctaat ttattagcaa
    12721 ttgcttggaa tattttccag tcaggtttag aatcacctaa tggtttcaat gcttggtata
    12781 aacgttgaat acgtcgctca gtattcgtaa aagtaccatc tttttctaat gaaggacttg
    12841 ctggtaagac aacatctgca aatgtagctg taaatgttaa aaattcatct tggactacca
    12901 tgaaatctaa tttttcaaaa gcagcttgta caaaattaat atttgaatct acaataccag
    12961 tatcttctcc atatagatat aatgaatgaa tttccccatc atggacacct tcaaccattt
    13021 catgattatc tttaccaact ttaggattca atttaacacc atattctttt tcgaatttag
    13081 cgcgaatatc gtccgcttca atactttgat agccagtaat tttatcaggc atactaccca
    13141 tatcactaca accttgaaca ttattatgtc cacgtaatgg atacgcacct gtaccaggtc
    13201 gacgataatt tccagtaact aataataagt ttgaaattgc tgtacttgaa tcacttccaa
    13261 tgtcttgttg tgtgataccc attgcccaac aaatgacaac ggactcagct ttggcacatt
    13321 cttcagcgaa cttaatcaac tctgcttctg gaataccagt agcctcttca gcgaatgcca
    13381 ttgtaaatgt ttctaatgac ttgtaatatt catcaaaatc ttcaacccac tcttcgataa
    13441 aggctttatc gtgtaagtca tgatcaatga tatatttcgt cactgcgctt aaccaagcca
    13501 aatctgtacc tggtttcggt tgataaaaac gatctgcgcg ttcagccatt tcatgttttc
    13561 taatatcaaa cacatgtatt ttttgtccaa ataatttttg agcacgcttc atgcgtgatg
    13621 caataaccgg atgcgcttcg gctgtattcg taccaatcaa tacagacatt gctgcttttt
    13681 ctaaatcttc aatactacct gagtcaccgc cgtgtccaac cgttctaaat aagccttttg
    13741 ttgcaggtgc ttggcaatat cttgaacagt tatcaacgtt attagtgcca ataacttgtc
    13801 ttgctaattt ttgcattaaa tacgattctt catttgtcgc tttagaagaa gaaatgaatg
    13861 atagtgcatc tgggccatac ttttctttaa tagatgtaaa attatctgca atgacattta
    13921 atgcttcatc ccattctact tcatggaact caccattttt ccttactaat ggtttcgtta
    13981 agcgttgatc tgaattaata tgtccccatg aaaatttacc tttaacacaa gtcgcaattt
    14041 tatttgctgg agaatcatgt gatggttgta cttttaaaat ttctctatct ttagtccaaa
    14101 cttcaaatga acagcccaca ccacaatatg tacacactgt tttagttttc ttaatacgct
    14161 ctttacgcat ttctgcttct gaatctgaga ttgcaaatag tggaccataa ccaggttctg
    14221 cttttttagt taaatcaatc attgccgcta atgaaccagg ttccgtatca gtcatataac
    14281 ccgcattacc ttccatgtta acttccatca tcgcattaca tgggcataca gttgcgcatt
    14341 gaccacaaga tacacatgac gattcattaa tcggtacgtc attatcccaa atgacacgtg
    14401 gatgctcacg atcccaatca attctaattg tttcatttac ttcaatatct tgacatgctt
    14461 ctacacaacg cccacataag atacattgat ttggatcata acgataaaat gggccgtaat
    14521 ctttttcgta tggcttctct ttatattcat acgtttgatg ctgaagtccc catgcatcca
    14581 tcgtattatg aatttcacaa tcaccattat tataatcaca tactgtacaa tacagcatat
    14641 gcttttctaa aattcgatca agcgcttctt tttgagcatc tttcacgtca ttattcactg
    14701 tatttactgt cattggacga tcaatcaccg tactacatga acgttcaatt ttaccgtcaa
    14761 tttcaacagt acatgtatca catgtttgaa ttggtcccat cgactcgtta taacaaattg
    14821 aaggtacaaa agtatcttgc gatttaatga attcaagtaa atttgtacct ggttctacaa
    14881 gataatcttt accatcaagc gtgaccacca aatgttcttg catactattc accccatcta
    14941 tatatttttc cgtaaatgac ttttaataaa ttgcctaatt tccacctaaa ataaagatgc
    15001 cccacacatc t
//
LOCUS       DAHHRR010000058        14905 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.058, whole genome shotgun sequence.
ACCESSION   DAHHRR010000058 DAHHRR010000000
VERSION     DAHHRR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14905
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.058"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            192..656
                     /gene="accB"
                     /locus_tag="NBI42_001545"
     CDS             192..656
                     /gene="accB"
                     /locus_tag="NBI42_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCD1777965.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAIPMPQVQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            656..2011
                     /gene="accC"
                     /locus_tag="NBI42_001546"
     CDS             656..2011
                     /gene="accC"
                     /locus_tag="NBI42_001546"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCD1777966.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRYEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            2026..2388
                     /locus_tag="NBI42_001547"
     CDS             2026..2388
                     /locus_tag="NBI42_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD1777967.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2448..2837
                     /gene="nusB"
                     /locus_tag="NBI42_001548"
     CDS             2448..2837
                     /gene="nusB"
                     /locus_tag="NBI42_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCD1777968.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            2854..4191
                     /locus_tag="NBI42_001549"
     CDS             2854..4191
                     /locus_tag="NBI42_001549"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCD1777969.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNFQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            4184..4414
                     /locus_tag="NBI42_001550"
     CDS             4184..4414
                     /locus_tag="NBI42_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCD1777970.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            4392..5273
                     /locus_tag="NBI42_001551"
     CDS             4392..5273
                     /locus_tag="NBI42_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCD1777971.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVLDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDATKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            5704..6156
                     /gene="argR"
                     /locus_tag="NBI42_001552"
     CDS             5704..6156
                     /gene="argR"
                     /locus_tag="NBI42_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCD1777972.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            6172..7851
                     /gene="recN"
                     /locus_tag="NBI42_001553"
     CDS             6172..7851
                     /gene="recN"
                     /locus_tag="NBI42_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCD1777973.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKKELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELIGDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            8002..9423
                     /gene="lpdA"
                     /locus_tag="NBI42_001554"
     CDS             8002..9423
                     /gene="lpdA"
                     /locus_tag="NBI42_001554"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCD1777974.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILALKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKNSLSARGVKFYEGIKLSENDININEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDANEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            9439..10431
                     /locus_tag="NBI42_001555"
     CDS             9439..10431
                     /locus_tag="NBI42_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCD1777975.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARERAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            10431..11414
                     /locus_tag="NBI42_001556"
     CDS             10431..11414
                     /locus_tag="NBI42_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCD1777976.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGKKGGVFGTTQGL
                     QQQYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNEWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFELDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            11427..12701
                     /locus_tag="NBI42_001557"
     CDS             11427..12701
                     /locus_tag="NBI42_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCD1777977.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNEKTEEIQAKVDEHTQKSTKK
                     ASAIVEQTSTANQNHPRNNGRYSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDLMSVI
                     ENGGTTAQSDKQVQTQSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            13053..13490
                     /locus_tag="NBI42_001558"
     CDS             13053..13490
                     /locus_tag="NBI42_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCD1777978.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            13504..14484
                     /locus_tag="NBI42_001559"
     CDS             13504..14484
                     /locus_tag="NBI42_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD1777979.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLDLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEALLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(14611..14790)
                     /gene="prli42"
                     /locus_tag="NBI42_001560"
     CDS             complement(14611..14790)
                     /gene="prli42"
                     /locus_tag="NBI42_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HCD1777980.1"
                     /translation="MLVISSQVKSFIPYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
BASE COUNT         5556 a         2039 c         2816 g         4494 t
ORIGIN      
        1 ttttaattaa gagttaagat gagtcaatgt taagtaataa atccacaagt gtttttaatt
       61 taaaatgtgg ttttattata actttgggat agacatataa tgtcgccagc aatatgaaca
      121 tgcttgaatt gaagagttgt ctcaagtaaa atagacgggt agatgaaaac aaactgaagg
      181 agtcagtaat aatgaacttt aaagaaatca aagaattaat tgaaattctg gataaatcaa
      241 ctttaacgga aatcaatatt gaagatacta aaggcaaagt gacgctaaag aaagaaaaag
      301 aaactgagat tatcacgcca caaatctcac aaatgccagt tgaagctgcg gcaataccta
      361 tgcctcaagt acaatcaact gatagcaata aaactgaagc tccaaagcca acttcagata
      421 atcacaaaac aattaatgca cctatggtag gtacatttta caaatcgcca tctccagatg
      481 aagaagcgta tgtgcaagtt ggggacactg tttcaaatga aacaacagtg tgtatattag
      541 aagcaatgaa actatttaat gaaattcaag cagaaatttc aggtgaaatt gttgaaatct
      601 tagtagaaga cggacaaatg gtagagtatg gccaaccgtt atttaaggtg aaataatgaa
      661 aaaggtttta attgcaaacc gcggtgaaat cgcagttagg attattcgcg cttgtcgtga
      721 tttaggcatc caaactgttg caatctattc tgaaggggat aaagatgcgc tacatactca
      781 aattgctgat gaagcatatt gcgtaggtcc cactttgtct aaagattcat atttaaatat
      841 tccgaacatc ttatctattg caacttctac aggttgtgat ggcgttcatc cgggttatgg
      901 ctttttagct gaaaatgctg actttgcaga attatgcgaa gcgtgccaat tgaagttcat
      961 tggaccaagt tatcaatcta tccaaaaaat gggtatcaaa gatgttgcta aggcagaaat
     1021 gattaaagcc aatgttccag ttgttcctgg tagtgacggt ttaatgaaag acgtctcaga
     1081 agctaagaaa atcgctaaaa aaattggcta tccggtcatc attaaagcta ctgctggtgg
     1141 tggcggaaaa ggtatccgtg ttgctcgtga tgaaaaagaa cttgaaactg gcttccgaat
     1201 gacagaacaa gaagctcaaa ctgcatttgg taatggtgga ctttatatgg agaaattcat
     1261 cgaaaacttc cgccatattg aaatccaaat tgttggggat agctatggta atgtaataca
     1321 tttaggagaa cgtgattgta caattcaaag acgtatgcag aaattggtgg aagaagcacc
     1381 ttccccaatt ttagatgacg aaacacgtcg tgaaatggga aatgccgcag ttcgtgcagc
     1441 gaaagctgta aattatgaaa atgcgggaac aattgagttt atatatgatt tgaatgataa
     1501 taaattttat tttatggaaa tgaatacacg tattcaagta gaacatcctg taactgaaat
     1561 ggtaacagga attgatttag ttaaattaca attacaagtt gctatgggtg acgtgttacc
     1621 gtataaacaa gaagatatta aattaacagg acacgcaatt gaatttagaa ttaatgctga
     1681 aaatccttac aagaacttta tgccatcacc aggtaaaatt gagcaatatc ttgcaccagg
     1741 tggatatggt gttcgaatag agtcagcatg ttatactaat tatacgatac cgccatatta
     1801 tgattcgatg gtagcgaaat taatcataca tgaaccgaca cgatatgaag cgattatggc
     1861 tggcattcgt gcattaagtg aatttgtggt tcttggtatt gatacaacta ttccattcca
     1921 tattaaatta ttgaataacg atatatttag aagcggtaaa tttaatacaa actttttaga
     1981 gcaaaatagc attatgaatg atgaaggtta ataggaggtc aatccatggt caaagtaact
     2041 gattattcaa attcaaaatt aggtaaagta gaaatagcgc cagaagtgct atctgttatt
     2101 gcaagtatag ctacttcgga agtcgaaggc atcactggcc attttgctga attaaaagaa
     2161 acaaatttag aaaaagttag tcgtaaaaat ttaagccgtg atttaaaaat cgagagtaaa
     2221 gaagatggca tatatataga tgtatattgt gcattaaaac atggtgttaa tatttcaaaa
     2281 actgcaaaca aaattcaaac gtcaattttt aattcaattt ctaatatgac agcgatagaa
     2341 cctaagcaaa ttaatattca cattacacaa atcgttattg aaaagtaatg tcatacctaa
     2401 ttcagtaatt aaataaagaa aaatacaaac gtttgaagga gttaaaaatg agtcgtaaag
     2461 aatcccgagt gcaagctttt caaactttat ttcaattaga aatgaaggac agtgatttaa
     2521 cgataaatga agcgataagc tttattaaag acgataatcc agatttagac ttcgaattta
     2581 ttcattggct agtttctggc gttaaagatc acgaacctgt attagacgag acaattagcc
     2641 cgtatttaaa agattggact attgcacgtt tattaaaaac ggatcgtatt attttaagaa
     2701 tggcaacata tgaaatatta cacagtgata cacctgctaa agtcgtaatg aatgaagcag
     2761 ttgaattaac aaaacaattc agtgatgatg atcattataa atttataaat ggtgtattga
     2821 gtaatataaa aaaataaaat tgagtgatgt tatatgtcag attatttaag tgtttcagct
     2881 ttaacgaaat atattaaata taaatttgat caagatccac atctccaatc tgtattgatt
     2941 aaaggtgagc tttcaaattt caaaaagcat tcaagtggtc atttatactt taatgtgaaa
     3001 gataaagaaa gcgttataag tgccatgatg ttcaaaggta gtgcttctaa attaaacttc
     3061 gaacctaaag aaggagatga agtcttatta gaagcacgtg tttctgtttt tgaacgtcgt
     3121 ggaaactatc aaatttatgt aaataaaatg caattagatg gtatagggaa cttatatcaa
     3181 aaattagaag cattaaagaa aaaattaact gaagaaggtt gttttgataa agcgaataag
     3241 aaatcaatac ctaagtttcc taaaaaaatt gcagttttaa cagcgagtac aggtgccgca
     3301 attcgagata tccattcaac gataaatagt cgttttccat tagctgaaca aatacaaatt
     3361 agtactttgg ttcaaggtga aaaagcaaaa gacgacatta ttgaaaaaat tgaatatgca
     3421 gatagtttag gtgtagatac cattattgta ggtcgaggcg gtggatccat agaagattta
     3481 tggaatttta acgaagaagc tgtcgtccgt gcaatatata attgtaagac accgattata
     3541 tcagcagtag gtcatgaaac agactttaca ttaagtgatt ttgctgcaga cattagagct
     3601 gcgactccaa ctcaagctgc tgttattgca acaccagacc aatatgaatt gctgcaacaa
     3661 atacagcaat atcagtttac attgactcgt ttcatcaaga aacatttaga acaacaacgt
     3721 aaacatgttg aacatttgtc atcgtattac aagtttaaac aaccaacttt attgtatgat
     3781 caacagatac aacgtcgaga tgatttagaa aagagactga aacaacaaat tcaggcaact
     3841 tttgaacaac agagacatcg attaatgctg ttgcaacaac gctataattt aaaagcgtta
     3901 ctaagtagtg ttaatcaaga acaacaaaat aattttcaat tgacaaacca acttgttaaa
     3961 ttattgaatt ctaaaatatt aagttataaa aatgatttga aaaataaagt agaaaactta
     4021 aacaatttaa gtccaactaa tacaatgttg cgtggatatg caattgttaa taaaaaagac
     4081 gaagtcatta cgagtactaa agatttaaca gaaaatgatc aattgacgtt aacaatgaaa
     4141 gatggcttag tagatgcaaa agttacgaaa gtaaggtgta ataatgacta aagaaacgca
     4201 aagttttgaa gaaatgatgc aagaattaga gcaaattgtt caaaaattag ataatgaaac
     4261 agtatcttta gaggaatcat tagatttata tcaacgtggt atgaaactat cagcagcttg
     4321 tgacacaact ttaaaaaatg ccgaaaaaaa ggtgaatgac ttaataaaag aagaagctga
     4381 ggatgtaaaa aatgacgaat ctaccgatga ataaattaat agatgaagtc aataatgaat
     4441 tatcggttgc gataaataaa tcagtattgg atactcagct agaagaaagt atgttgtatt
     4501 cattaaatgc tggaggtaaa cgcatccgac cagttctgtt attactcact ttagattcac
     4561 taaataccga gtatgagtta ggtatgaaga gcgcaattgc actagaaatg attcatacat
     4621 attcacttat tcatgatgac ctaccagcga tggataatga tgattatcga cgagggaaat
     4681 taacaaatca taaagtatat ggtgagtgga ctgcgatatt agcaggtgat gctttattaa
     4741 ctaaagcatt tgaacttatt tcaagtgatg atagattaac tgatgaagta aaaataaaag
     4801 ttctacaacg gctgtcaata gcaagtggtc atgttggaat ggtcggcggt caaatgttag
     4861 atatgcaaag cgaaggccaa ccaattgatc ttgaaacttt ggaaatgata cacaaaacaa
     4921 aaacaggagc actattaaca tttgcggtta tgagtgcagc agatatcgct aatgtcgatg
     4981 atgcaactaa agaacattta gaaagttata gttatcattt aggtatgatg tttcagatta
     5041 aagatgattt attagactgc tatggtgatg aagcgaagtt aggtaaaaaa gtgggcagcg
     5101 atcttgaaaa taataaaagt acttacgtga gtttattagg aaaagatggc gcagaagata
     5161 aattgactta tcatagagac gcagcagtgg atgaactaac gcaaattgat gaacaattca
     5221 atacaaaaca cttattagaa atcgttgatt tattttatag cagggatcac taaaagcaaa
     5281 tatatcgatt agcaacaatt gtaaagtttg aaatgtctca aaaggcatat tcaactgacg
     5341 ttgtttgtta atcgattttt ctcatgttct aacttaaaat tatgttgaag actttcatct
     5401 aaaaatagaa ttttcaattt ctatacgaca atattcgtat ctgtgatatc aatgtgattt
     5461 taattaaata tgttcaaaca attgacgctt attaaatcta ataattagca agtttattac
     5521 acatatattc atttttatac atattttaag caattgttat gcgtaatatg aaaaatattt
     5581 ataaatagat aatcaagata gataaaccat gtttaccctt tttacgtaaa ggtgtttagt
     5641 agtcgtaaat cagtgcatgt gttaaaataa atgtataaat attcgtaatc agaggtgttt
     5701 actgtgccca aaaaatcggt taggcatatt aaaattagag aaattatttc aaatgaacag
     5761 atagagacac aagatgaatt agttaaacga ttaaacgatt atgatttaaa tgtcactcaa
     5821 gcaactgttt ctcgtgatat taaagaacta caacttatta aagtacctat accttcaggt
     5881 caatatgttt atagtttacc aaatgataga aaattccatc ctttagaaaa attgggacgt
     5941 tatttaatgg attcctttgt taatatagat ggtactgata atttacttgt tctaaaaaca
     6001 ttacctggta atgcacaatc tattggagct atattagacc aaatcaattg ggaagaagta
     6061 ctaggcacaa tttgtggtga tgatacttgt ttaattattt gtcgaagcaa agaggcaagt
     6121 gatgaaatca agtcaagaat tttcaatttg ttataaggat gcgatgagtt tatgttacaa
     6181 accttatcaa tcaagcaatt tgctattatt gaagagttag aaattcaatt ttctgatggt
     6241 ttaactgttt taagtggtga aacgggttca ggtaagtcaa ttattataga tgcaattgga
     6301 caattaattg gaatgagagc ttcatctgat tttgtaagac atggtgaaaa aaaggctgtc
     6361 atcgaaggta tatttgatat tgacgagagt aaagatgcta tacatatatt gaaaaatatg
     6421 gatattgatg tagatgaaga ttttttatta gttaaacggg aaatttttag ctctggtaaa
     6481 agtctttgta aaattaataa tcaaacagtt actttacaag atttacgaaa agttatgcaa
     6541 gaattattag atattcatgg ccaacatgaa acgcagtctt tgttgaaaca aaaataccat
     6601 ttaacattat tggataatta cgcagaatca cgttatcaag atttgttgga taaatatcac
     6661 caaacttttc aaaattataa agccaaaaag aaagagttag aagatttaga atcagcagac
     6721 caagcattgc tgcagcgttt agatttaatg aaatttcaat tagaagaatt gtctgaagca
     6781 catctaaaag aaggcgaaat agagcaactt gaaatagata ttaaacgcat tcaaaactct
     6841 gaaaaattaa gcttagcgct taataacgca catatgacat taacggatga aaatgctatc
     6901 actgatcgtt tgtatgaact aagcaaccat ttgttaacaa taaacgatat agttccaaac
     6961 aaatatgaca aattaaaaga agatattgat caattttatt acattttaga agatgcaaaa
     7021 catgagttat acgatgaaat ggctaataca gaattcgacg aacaagtgtt aaacgagtat
     7081 gaatctcgta tgaatttgct gaataattta aaacgcaaat atggaaaaga tatttcagaa
     7141 ttaatcgcat atcaagaaaa acttaataat gaaatcaata aaattgaaaa ctatgaacaa
     7201 agtacatcgc agttacgaga agaaataaat gcattgtata atcaagttat agaggttgga
     7261 caagcgctat caaagcagcg tcgtatagtc gctagggagt taagagacca cattgtatcg
     7321 gaaattcaaa acttacaaat gaaagacgca aatcttgaga tttcatttaa aaaattagaa
     7381 gaaccgaata ttgatggaat cgaatttgta gaatttttaa tcagtcctaa taaaggggaa
     7441 ccattaaaaa gtttaaataa aattgcgtca ggtggagaac tttctagaat tatgttagcg
     7501 ttaaaaagta tttttgttaa atcgagaggt caaactgcaa ttttattcga tgaggttgac
     7561 tcaggtgtat ctggacaagc tgcacaaaaa atggcagaga aaatgcgaga tatagcagaa
     7621 tatatacaag ttatttgtat ttctcacttg ccacaagttg catcgatgag cgaccatcat
     7681 ttattaattt cgaaatcatc aaaagatgat cgtacaacaa cacaagttca agagttaatt
     7741 ggcgatgata aagtagatga aattgcacga atgatttcag gtgcaagtgt tactgattta
     7801 actcgagaaa atgcaagaga aatgatacaa cacaatcaaa gacgtagata atttccaaag
     7861 tgtgttgaat actcatttgt ggatttattt atgtgcattc aaactaaata gacaatcatg
     7921 taaaataggt aattaatgga catgaaaact atgtattaat aaattttcaa tggatatata
     7981 tcattagtta ggagtaagaa catgtcagag aaacaatatg atttagtcgt tctcggtgga
     8041 ggtactgcag gatatgttgc tgcgatacgt gcgtctcaat taggtaaaaa agtggcaata
     8101 gtagaacgac aactattagg aggtacttgt ctccataaag gttgtattcc tactaaatcg
     8161 ctgcttaaat ctgctgaagt atttcaaaca gtaaaacagg cagcaatgtt tggtgttgat
     8221 gtcaaagatg ctaatgttaa ttttgaaaat atgttggcac gaaaagaaga cattattaat
     8281 caaatgtatc aaggtgtaaa gcatttaatg caacacaatc acattgacat ttataatggc
     8341 acgggacgta ttctaggtac atctatattt tcacctcaaa gtggcacaat ttctgtcgaa
     8401 tatgaagatg gtgaatcaga cttattaccc aaccaatttg tattaattgc aactggttct
     8461 tcgcctgcag agttgccatt cttatcattt gatcatgata aaattttatc tagtgatgat
     8521 atattagcat taaagacgtt accatcaagt attggtatta ttggtggtgg tgttatcgga
     8581 atggaatttg catcattgat gatagattta ggtgttgacg taacagttat agaagcaggt
     8641 gaaagaattt taccaactga aagtaaacaa gcttcacaac tattaaaaaa ttcattgtca
     8701 gcacgtggtg ttaaattcta tgagggaata aaactttctg aaaatgatat aaatattaac
     8761 gaagatggtg ttacgtttga aatttcctca gacataatta aagtagataa agtgttgcta
     8821 tcgattggta gaaaacctaa cacatcagat ataggtttaa acaacactaa aataaaactt
     8881 tcaacatcag gacatatttt aacgaacgaa tttcaacaaa ctgaagataa acatatttat
     8941 gcagctggtg attgtattgg aaaattacaa ttggcacatg ttggatcaaa agaaggtgtt
     9001 gtggctgtcg atcatatgtt tgaagggaac ccaatcccag taaactataa catgatgcct
     9061 aagtgtattt attcacaacc tgaaattgct tctatcggtt taaacattga gcaagcaaag
     9121 gcagagggaa tgaaagttaa aagttttaaa gtaccattta aagcaattgg taaagcagtg
     9181 attgatagcc atgacgcaaa cgaagggtat agcgaaatgg tgattgatca atcaactgaa
     9241 gaaattgtgg gtattaatat gattggtcca catgtaacag aattgattaa tgaggcatca
     9301 ctgttacagt tcatgaatgg ctcggcatta gaattaggac taacaacaca cgcgcatcct
     9361 tccatctctg aagtgttgat ggaattagga ttgaaagcag aaagtagagc tattcacgta
     9421 taaataggag gatttaacat gattgattat aaatcattag gccttagcga agaagaccta
     9481 aaagtaatat ataaatggat ggatttaggt agaaaaatag atgaaagact gtggttattg
     9541 aaccgtgcag gtaaaattcc atttgttgta agtggtcaag gacaagaagc aactcagatt
     9601 gggatggcat acgcattaga agaaggtgat attacagcgc cttattatag agatctagct
     9661 ttcgttactt atatgggtat ctcagcgtat gatacgtttt tatcagcatt tggaaagaaa
     9721 gatgatgtta actctggtgg aaaacaaatg ccatcacact ttagtagtag agctaagaat
     9781 attttgtcac aaagttcacc ggtagcaacc caaatacccc atgctgttgg tgctgcttta
     9841 gctttaaaaa tggatggtaa gaagaaaatt gcaacagcaa ctgttggtga aggtagctca
     9901 aaccaaggtg acttccatga aggtttaaac ttcgcgggtg tacacaaact tccttttgta
     9961 tgtgtcatta taaataataa atatgcaatt tcagtacctg attcattaca atacgcagct
    10021 gaaaaattat cggatagagc acttggttac ggtatacatg gtgaacaagt agatggtaat
    10081 gatccattag caatgtataa agcgatgaaa gaagcaagag aacgagctat ttctggtcaa
    10141 ggttcaactt tgattgaagc agtaacttct cgtatgacag ctcattcatc ggatgacgac
    10201 gaccaatatc gtacgaaaga agagcgtgaa gcgcttaaaa aagcagactg caacgaaaaa
    10261 ttcaaaaaag agttgctttc agctggcatt atcgatgatg cttggttggc agaaatagaa
    10321 gcagaacata aagacattat caataaagca actaaggctg ctgaagacgc gccatatcca
    10381 agtgtcgaag aagcctatgc atttgtgtac gaagaaggga gtcttaacta atggctaaat
    10441 tatcttattt agaggcgatt cgccaggcac aagacttagc attacaacaa aataaagatg
    10501 tatttattct tggtgaagat gttggtaaaa aaggtggcgt atttggtact actcaaggac
    10561 tgcaacaaca atatggtgag gacagagtta tcgatacacc attagctgag tcaaatattg
    10621 tcggaacagc cattggcgca gctatggttg gtaaaagacc aattgctgag attcagtttg
    10681 cagattttat cttacctgca acaaatcaaa ttattagtga agccgcgaaa atgcgttatc
    10741 gttcaaataa tgaatggcaa tgcccattaa cgattcgtgc accatttggt ggcggtgttc
    10801 atggtggact ttatcattca caaagtattg aaagtatatt tgcttcatca ccaggtttaa
    10861 caattgttat accttcaaca ccgtatgatg ctaaaggtct attattatct tcaattgaat
    10921 caaatgatcc tgttttatac tttgaacata aaaaggctta tagattctta aaggaagaag
    10981 tgccagaaga atattacact gtacctttag gaaaagcaga tgttaaacgt gaaggtgaag
    11041 atttaacagt attttgttat ggattaatgg tcaattattg tttacaagca gcggatattt
    11101 tagcagcaga cggtatcaat gttgaagtcg tagatttacg aactgtttat ccattagata
    11161 aagaaacaat tattgatcgt gctaaacata caggtaaagt tttacttgta actgaagata
    11221 atttagaagg tagtattatg tctgaggtgt cagcgattat tgcagaacat tgtctgttcg
    11281 aactagatgc accaatcatg cgtttagctg ctccagatgt accatctatg ccattttcac
    11341 ctgtattaga aaatgaaatt atgatgaatc cagaaaaaat cttaaataaa atgcgtgaat
    11401 tagcagaatt ctagggaggg aaagtcatgg aaataacaat gcctaagtta ggtgagagtg
    11461 ttcatgaagg taccattgaa caatggttag tttctgttgg tgatcatatt gatgaatatg
    11521 aaccattatg tgaagttatt acagataaag tgacagctga agtcccttcc acgatatcag
    11581 gaacaattac agaaatttta gttgaagcgg ggcagacggt agctattgat acaattatct
    11641 gtaaaattga aactgctgat gaaaagacaa atgaaaaaac tgaagagata caagcaaaag
    11701 tggatgagca tactcagaaa tctactaaaa aagctagtgc aatagtggaa cagacatcta
    11761 ctgctaatca aaatcatcca cgtaataatg gtcgctattc acctgttgta tttaaacttg
    11821 cttcagagca tgacattgat ttatcacaag ttgtaggtag tggatttgaa ggtcgtgtaa
    11881 ctaagaagga tttaatgtca gttattgaaa atggtggtac cacagctcaa tctgacaaac
    11941 aagttcaaac acaatcaaca tcagtagata catcaagtaa ccaatcatct gaagacaata
    12001 gtgaaaacag cacaatacca gtaaatggtg tgcgtaaagc aattgcgcaa aatatggtta
    12061 atagtgtaac agagattcca catgcatgga tgatgattga ggttgatgct acaaatcttg
    12121 tgaatacgag aaatcattat aaaaacagct ttaaaaataa agaaggatat aatctaactt
    12181 tctttgcttt ctttgttaaa gctgtagcag atgctttaaa agcatatcct ttattaaata
    12241 gtagctggca aggaaatgaa attgtcttac ataaagacat taatatttca attgctgttg
    12301 ctgatgaaaa taaattatac gtacctgtga ttaagcatgc agacgaaaag tcaatcaaag
    12361 gtatagctag agaaattaat actttagcaa cgaaagcgcg taataagcaa ttgacagctg
    12421 aagatatgca gggcggtaca tttacggtaa ataatactgg tacatttggt tcagtatcat
    12481 caatgggtat tataaatcat cctcaagcag cgattttaca agtagaatca atcgttaaaa
    12541 agccagtagt aattaatgat atgattgcaa ttcgtaacat ggttaattta tgtatttcaa
    12601 ttgatcatcg tattttagat ggtttacaaa caggtaaatt tatgaatcat attaaacagc
    12661 gtatcgaaca gtatacttta gaaaatacaa atatatatta gtgataacat agatgcatct
    12721 atcgacaact tgttttatct tgttcttgtc gatggatgta ttattttttg gcaataaaat
    12781 atttgcaata tattcaaaaa aagataaaga acaataatca acatggttga atgcattttt
    12841 gcagtaaagt caaaataaga catcatactt gaaacatatt aatgaaaaca ggtgaacaaa
    12901 ttagtttcga tgattttaat cacaataatg tttggtatat tgttaaaatt gtgtctaaat
    12961 ataggtgtga ttcagattag tttattgaac aatatgttat taattagtag aatgaggata
    13021 gtttaaatat aaagggatag gtgattgaac ttatggacat gaatttcgat ttatacatga
    13081 acggtgttgt agaacaagca aggaatgaaa ttgaatctgc gggatatgag caattaacta
    13141 ctgcagaaga tgttgacaaa gttcttaaac aagatggtac aacactagtt atgatcaatt
    13201 ctgtatgtgg ttgtgcaggt ggtatcgcaa gaccagcagc atcacatgct ttacattatg
    13261 acgtattacc tgatcgtcta gtgacagtat ttgctggaca agataaagaa gcgacacaaa
    13321 gagcgcgtga gtacttcgaa ggttatgcgc cttcaagtcc gtcatttgca ttagtaaaag
    13381 atggaaagat tacagaaatg attgaaagac atcaaatcga aggtcatgat gtgatgaacg
    13441 taattaatca attacaaaca ttattcaata aatattgtga agaaagataa gaggcgctaa
    13501 cccatgttaa agttaaatcc ttacaagatt ggatttagaa caataaaaac agcagtgggt
    13561 atgactttag gtgtaattat tagtaagttg ttagatttag ataattatgc ttcaagcgcc
    13621 atattagtcg tattatgtat taaacataca aaagtacatt cgctacaagc gattatttca
    13681 agattagtat catgtttttt agtattgttt ttaggttcag caatatttag tttattaggt
    13741 cagagtccaa ttgtactcgg tattatcgta ttgttattta taccattaac tgtcgtatta
    13801 aaagtacaag aaggtgtcat tacgagttgc gttatattac ttcatgtttt taatgcaaaa
    13861 tcaattgatg cacatttaat tgttaatgaa acattattac tgttaattgg actaagcatt
    13921 gcatttacaa tgaatttaat gatgccaagt ttagacaaac aactagacga atacaaatgt
    13981 aaaattgagc aacaaattgc tgatattttt agtaaatata gttatatttg tgaaaaatat
    14041 gaagatacca ttgcgattga atttgaagcg ttacttttaa atattaaaaa ggcgaagtct
    14101 atcgcgttcc gagatgttaa aaatcatttt gttagaaacg aaaattcata ctatcattat
    14161 tttgatatgc gagaagagca agtggaattg ttaatgagaa tgaaaccgct catcgaaagt
    14221 atctgtcata aagatccact tttaaatgat gttgcgtctc tgttgaaaga aattggtcta
    14281 aatgtgaata gtaatgacta tacagcaatg cgtttacaca atttgtatga gacgcgtttg
    14341 aaattggatg aactgccatt gccaacaaca cacaaagcgc ttaactcgag agcaagtatt
    14401 attcaaatat taaatgaatt agaagaatat ttacaaatca aatcgcaatt tggttcattg
    14461 aaaatgcata gtgaagtagc ttaaaaaata atccatccgt atgacccatg tttgaaagta
    14521 ttcgttgcaa tacgataact tattaataca tgcgtttata acggatggat ttttaattgt
    14581 atgattttgt ataggtagtt gagatatagt ttataaaatt ggaatgatac tcgttaataa
    14641 caatgctaat acaactgata caagcataac gataatgata attcttaaaa ctttattttg
    14701 cattttgtgt gcctccaaat gaataacctt tgtattaata attacgacat aaggtataaa
    14761 acttttaact tgagacgata taactagcaa tcatcataaa ggaatattat aaaaaacatg
    14821 agtaagtttg cttatgtcat tttcctcatt atacgatatc tttgaaaaat caatcaatat
    14881 atttttgaca aagcttagtt tgagt
//
LOCUS       DAHHRR010000059        14767 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.059, whole genome shotgun sequence.
ACCESSION   DAHHRR010000059 DAHHRR010000000
VERSION     DAHHRR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14767
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.059"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(186..794)
                     /locus_tag="NBI42_001561"
     CDS             complement(186..794)
                     /locus_tag="NBI42_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCD1777981.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEDG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            1193..1840
                     /locus_tag="NBI42_001562"
     CDS             1193..1840
                     /locus_tag="NBI42_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCD1777982.1"
                     /translation="MEQLHQVGFMKSFVLFWKNYVNFTGRSRRSEYWYMMLWHLILMV
                     PAVCVLIISLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTAFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSSIKNQPVTSEAPEKTGTSETPKTPEETDNQL"
     gene            2071..2970
                     /locus_tag="NBI42_001563"
     CDS             2071..2970
                     /locus_tag="NBI42_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1777983.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDMSLELESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETSLPDLTHIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            2963..4195
                     /locus_tag="NBI42_001564"
     CDS             2963..4195
                     /locus_tag="NBI42_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1777984.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHNLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGIMAYVLFAAILGSLTSRIEDLNQSLMPMTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIHEVILSSLISIVMVAVLIWIAIKTYKES
                     VLTFERGLFNSMKRVFKK"
     gene            4179..4364
                     /locus_tag="NBI42_001565"
     CDS             4179..4364
                     /locus_tag="NBI42_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1777985.1"
                     /translation="MYLKNKIDESDTHLSYKMMVKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            4413..4739
                     /locus_tag="NBI42_001566"
     CDS             4413..4739
                     /locus_tag="NBI42_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCD1777986.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGVILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            4769..5788
                     /locus_tag="NBI42_001567"
     CDS             4769..5788
                     /locus_tag="NBI42_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCD1777987.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDVLDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(6024..6677)
                     /locus_tag="NBI42_001568"
     CDS             complement(6024..6677)
                     /locus_tag="NBI42_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCD1777988.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPSDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMIRYALNNPFLSPTRRDKLEKMYNKTLK"
     gene            6848..7534
                     /locus_tag="NBI42_001569"
     CDS             6848..7534
                     /locus_tag="NBI42_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCD1777989.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNIIGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKMVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(7599..8855)
                     /gene="sdpC"
                     /locus_tag="NBI42_001570"
     CDS             complement(7599..8855)
                     /gene="sdpC"
                     /locus_tag="NBI42_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCD1777990.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFAFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEENNHTEAEKSSSNIKDAEKTGVATASTVGIAKNDTEKTAADEPSIHEGTEKTEP
                     QHHIDNQTESNHNEHHDITSESVESAESVKHAPQSDDLTNDSNEDEIQSLKEPATYKE
                     DRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            9145..10080
                     /locus_tag="NBI42_001571"
     CDS             9145..10080
                     /locus_tag="NBI42_001571"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCD1777991.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSRPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCEALVDYGNVYHDYEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIGYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(10814..11698)
                     /locus_tag="NBI42_001572"
     CDS             complement(10814..11698)
                     /locus_tag="NBI42_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1777992.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            11858..13519
                     /gene="hutU"
                     /locus_tag="NBI42_001573"
     CDS             11858..13519
                     /gene="hutU"
                     /locus_tag="NBI42_001573"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCD1777993.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            13519..14757
                     /locus_tag="NBI42_001574"
     CDS             13519..14757
                     /locus_tag="NBI42_001574"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCD1777994.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGQHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTLSFKA"
BASE COUNT         5053 a         2048 c         2896 g         4770 t
ORIGIN      
        1 ataattataa ataattctat cgttatattt tataattaag aacaagttca aaaagaggag
       61 atgaatcaaa aaatatgatg gatacaaaga aaaacaatca acccatccgc atgttgacgg
      121 ctgattgatt gacttataag gagatagatt tcattttcat gatagtaatc aaagaaagat
      181 gacatttatt tccaagtatc ttcgggaaac atacaatctg atttacgcat gcgagacaca
      241 aatggattac ctttcactgt gtaacgtaaa gatttatgtg tccaatcacc tttatttgga
      301 ataccgattc gtggactagc aataatatct ttaggatatt tacgattctt agtatcaata
      361 gacaatctac agtcatttaa cgtagcgcca tcgatagccc gtggaatgtt aaatgcctta
      421 gtccattttc ctgggccatt cgttacctcg tagcctttct tacctctgtt acggaacata
      481 gcggataaac cgtcttctgg ttcaattgcg cggataagta cgccttcagg tataccttca
      541 gattttgtta caaaattaat gagtaaatgc gtatgcatga catgtgcata aattgtacca
      601 ccacgtttat ataaggacgt aactttaggt gttattttac caccatagcc atgagccgca
      661 cgatcattca aacctaagta tgcttccgtt tccacgatgt agcctgtata cgtttgagtg
      721 gtatcctgat aaatcacttt gacacctaat aagtttttag caatttgtct tgtatcatta
      781 ttaacgaaat ccataatatt ctcctcgcaa ttatatgatt aatttaaata aaaatgaaaa
      841 agatattttt attgtggtaa atgtttgata aaatagtatt gttaagaatt ataaaaatgt
      901 aaagtatata ctttgttatg gaatatttta tttgtagaga ttgagtctct tttgaacgcg
      961 aacattgcag tgtgacatat ttttagtata gttaacgaag cgttgacttt caaatgagtc
     1021 tccatataga tgcgtacttg tagcgggttt tcatttaact agtgatgaaa tacaaagttc
     1081 attttccaaa gtgaatgacg attgaaatga agtggcttga taatgaatct tgtaaattat
     1141 aacggtaata ataattaaaa atataaatat gattaacgaa agggagatta caatggaaca
     1201 attacatcaa gtaggtttta tgaaatcatt cgtattattt tggaaaaatt atgtaaattt
     1261 tacggggaga tcaagacgta gtgaatattg gtatatgatg ttgtggcatt taatacttat
     1321 ggttccagct gtgtgtgtgc taataataag cctatttcta ttctttattt ctatagcggc
     1381 agataacgaa acattaatag ttatgagtat attgatgatt atccttacat ctgtgtatac
     1441 ttttgtctat agtttggcaa cgtttattcc aaatttagcg ttgtgcgttc gaagatttca
     1501 cgatatatca agaacaatgt tatttccaat gattatgaca gcattcagta ttatatttta
     1561 tatagtatta cagattatcg atagttatta tgacagtgat tttaccttaa tgccgatggg
     1621 tcttaatatt ttgttagtac ttctttattt tgtctattta ggcctaacaa ttgttatgat
     1681 agtattccta tgctttgaca gtaagccggc aaataaatat ggcgaaagcc caaaataccc
     1741 atcttcaatt aaaaatcaac cagtgacatc tgaagcaccg gaaaaaacgg gtacatctga
     1801 aacaccgaaa acaccggaag aaacagataa tcaattgtaa ggatataaat atctcggcac
     1861 aatgatgctg ggatattttt ttaccaataa agcaataagg gcatggaaaa tggggagtta
     1921 atgctcatga tacttaacat gcaaagaaga tactatttaa atatttcaat ttcagaaatt
     1981 ttaaaaataa attcactttt aaaatattgc atttatagag aatagaaatg ttatattaag
     2041 ataaatacat attgttttag gaggcaaagt atgggacttg taattgaaca tgttacgaaa
     2101 cgttttggca agatgacggc agtaaatgat atgtcattag agttagaatc aggcaaaatg
     2161 ttaggttttc taggaagaaa tggtgcaggt aaaacaacga catttcgtat gattttaggc
     2221 ttaagtgaac caactgaagg acacattacg tataacggta aaaaactaga taaaacaatg
     2281 tacaatcgta ttggttattt accggaagaa cgcggtttac atgggaaact gacagttgaa
     2341 gaagaattga aatatttggc aactttaaag ggaatgtcaa aaacagaaat acaacagcaa
     2401 atatcgtatt ggcttgagcg ttttgatatt actgaaaacc gtaaaaaacg aattgatagt
     2461 ctatcaaaag ggaatcaaca aaaaattcag ctgttagcaa gtatgttaca taaaccagaa
     2521 ttactaatat tagatgagcc tttcagtggt ctagacccag ttaatgttga gttattaaaa
     2581 gaagcagtta aagacttaaa tgattggggc agtacaatcg tttatagttc tcaccgtatg
     2641 gagcatgttg aagaattatg tgatgatgtt tgtattttag ataaaggtca acttgttgtt
     2701 tctggtgata tcaatcatgt acgtgcatct aatggtaaca agaaagttgt cattgaatca
     2761 gaaacgtcac taccagattt aacacacatt agaggcatca ttcatagtga aaacatgaag
     2821 caaggtcttc agttgacgat tgagaatgaa gacgtagcca aagatattta tcaagttgta
     2881 gcacatcaag gttatgttaa acgattccaa gttgtagaac catcattaca agatatattc
     2941 attgagaagg ttggtggcaa ggatgcataa gttttgggcg acatttactt tgacatatat
     3001 caaaaaaatt aaagctaaat catttgtaat ttttatgatt atcattgctg ccttaatgat
     3061 tggcctatct aacattgata agattattaa catgtttgat gatggtccag ataaaattgg
     3121 tgtagcagca ccgaatgaac aaatctataa ggttttcaag caacaggcga atacatttca
     3181 tagtgatgct aagtttacta aagtttctat agaagatgct gaaaaagaag tgaaaaaaca
     3241 taaccttgat aaagcttata ttatcaaggt taatcaaaat cgtacgttac aagggacaat
     3301 cattagtgag aagcgagtaa gtcatgaaga ttcacaaaaa gtacaagcac tacttacagc
     3361 tattcaaaca aatatggtag ccggggaatt aaatatcaac aaagaagact tacaaaaatt
     3421 acaagcacaa agtaaagttg ataataaggt aatttctaat gatgaagtcg ataaagtatc
     3481 agaaggacaa aagattttca actatgcttt agcttatggc attatctttc taatgttctt
     3541 tattgtttta aattatgcat ctcaaatagc tatggaaatt gctagtgaga aaacatcgcg
     3601 tgtcattgaa atgattatta caagtatttc accaatccaa catatttttg ctaaaatttt
     3661 aggcgttata gcagttgccg tgactcagat tattttaatt gtattgatgg cagttatatg
     3721 catttttgcc tttgacttaa aagacttgct acaaggtttt aacgtggaaa tgaatcaatt
     3781 gtcttggcaa attattattg tcggaattat aagtagtatt gttggaatta tggcgtatgt
     3841 attatttgca gccattctcg gatcattaac atctagaata gaagatttaa atcaatcttt
     3901 gatgccaatg acattacttg gtatgattgc attttatatc gcgatattca cgataaataa
     3961 tcctgatggg caacttgctt taatcacaag ttatattccg tttttagcgc catttcaatt
     4021 agttgtacgc gctcaaactt cagggcttca aatacatgaa gtcatcttaa gttcattaat
     4081 ctcaattgta atggtagctg tcttgatatg gattgcaatt aaaacatata aagaatcagt
     4141 acttacattt gaacgtggct tatttaattc aatgaaacgt gtatttaaaa aataaaattg
     4201 atgagagtga cacacattta tcatataaaa tgatggtgaa agcgtgttac tcttattttt
     4261 ttatatttag ctatagaata atgtctaaaa aagtgtacaa tatgaatgtg aaaacgaact
     4321 ttttaaaatg ggaatgtgaa gtaagaagaa atgtaaacaa atgatatgta gccgatagat
     4381 tattggtagc aaatgtgtga tggagtgatt tcatgttata tccgattttt atatttatat
     4441 tagctggctt atgtgaaata ggtgggggat acctgatttg gctgtggctt agggaaggac
     4501 agtcttcact tgttgggcta ataggcggtg tgatactcat gttatatggt gtcattgcga
     4561 catttcaatc atttccatca ttcggaagag tatatgcagc atatggtggt gtatttatca
     4621 tcatgagttt gatttttgcg atggtcgtag ataaacaaat gccagataaa tatgatgtta
     4681 tcggtgcaat catttgtatt gtaggtgttt tagtaatgtt gctacccagc agagcttgaa
     4741 cacttgtggt tagaagggag aagtaaaaat gatagttgaa gttgagcacc aaagacaagg
     4801 tgtagattta atcaaaattg ataatgatga aactaaaatt atttttacga attatggagc
     4861 aagagtagtg tcttggaaat atcatgataa taatattgtt cttggaaatg ttgttgaagc
     4921 ggatgaattt tatcatagta atccttttaa atttggtgct tctataggaa gatatagcgg
     4981 tagaattgat aatgctaaat ttaagttgaa aggtaaagaa tatcagttag aaaaaaataa
     5041 cggcgaacat catttacatg gtggatgtca tggtttggac aataagttat ttgattatga
     5101 aattagaaat gaaattgctc aaattaaagt gatatttaag acggtgttga aatcagctga
     5161 tgatcatttc ccaggtgata tcgatgtgac gattacacat atttatgatg cggatcacca
     5221 atggtcgatt gaatacgaag cagttgcatc agaagacaca ttatttagtc ctacgaatca
     5281 tgtgtacttc aatttgaatc gagataataa tgtcgttgat aatcatcgca tttcaagtaa
     5341 tcaattagat atgtatgtat tagatgagcg aaatattgtt acaggtgatg ttctagattt
     5401 acatgaagtg tttgaagata acaagataaa attgagtgat atttttacat cacaacatgc
     5461 gcaattaagc caacaaatga cacgttttgg tggtctcgat cacccattta cagttggtga
     5521 acataaaatg tatgttgaaa atcatgaatt tatgttagag gtcgatacag atatgcctca
     5581 tgttgtattc tttactttta atcagcctga tgagtgggat agtccattta atatttataa
     5641 accacattct ggctttacat tagaaacaca atttttacca aatgatatta atttatatgg
     5701 agaaaaagca ccgtcaattt taaaagctgg tgaagtgttc aattcgaaaa caagtttccg
     5761 tatcgaagaa aaagtagaaa atgattaaaa gtatcggtat ttatataata tttatgttat
     5821 ttggcggaat tttgaatagg ttatcaagaa tcgtatcaaa tgaagtaaca tcattaatat
     5881 acagtttgaa ataaagaaaa tatataacat aaaaaaggaa tcaatagcat cagtagttat
     5941 agataagact attatacaaa cgatgtgtaa gttgattcct ttttgttttg aaaagttcat
     6001 ttaacactta taatgaaagg taattatttc aatgttttgt tatacatttt ttcaagttta
     6061 tcgcgtcttg ttggtgacag aaatggattg tttagtgcat atcgtatcat atcttgattt
     6121 tgtctatcat tataataaag gtcattaagc tgttgtacag ataaacgatg ttcgattggt
     6181 gcttcttgta ttaagtaaag ctgatcactt ggagatacat agccttgttt taatacccgg
     6241 aaatagaaac cagttttacc agatgtagac atgcgcttca ctaaatcagg aatattatat
     6301 tttgcttgaa ttttccagca tggttctcta atttcagaaa cctcaattaa cgcttcacct
     6361 aaacgatatt gattaccaaa gtaaacatca gattcgtcta aataatcaaa tgttaaattc
     6421 tctccaaaca tcgcatgagt aggtagtgta ggtaaatcat cttgatacag tgcatagtta
     6481 tgtttactaa acccacaaac tgctttatgt ggcccaccat gatctttata agcctgttca
     6541 tcaccagtga aaccagttga agataaccat gttgtttgtg aaatcttagt tttatctaaa
     6601 gcagagcgca tcggtctttt cgagctataa ggcaaatctt ggatttttcc tgtcgaaatt
     6661 gcgtgaattg atatcatatc attctcctta acgttaagtt aaagtatatt gtactataaa
     6721 acatttttag aattgtaaat tctgattgat atgtttaaaa aaatgttaga aataagggta
     6781 tgttaattta tacgagtcat gtaaaatatg aattgaatag attacataaa tatgggaggc
     6841 taccttcatg aaagatgtca aagcactcaa gttaatgaca ttaaatgatg ttttaagtca
     6901 aatcaatggt gacatgacgc tgggaattgg tacaggaagt acaatggaat tactcttacc
     6961 tcaaatggcg caactaatta aagaacgtgg ttacaatatt ataggtgtat gcacttctaa
     7021 taaaattgca tttttagcaa aagaactagg tattaaaata tgcgaaatca atgatgttga
     7081 tcatatcgac ttagcaattg atggtgctga tgaagtagat ccatctttaa atataattaa
     7141 aggtggcggt ggtgcgctgt tcagagagaa agttatagat gaaatggcat cgcgttttgt
     7201 tgtggttgtc gatgaaacga aaatggttca atacttaggt gagacgttta agttaccagt
     7261 agaggtggat aaatttaact ggtatcacat attacgtaaa attgaatcat acgctgatat
     7321 aaaagtagaa cgccgtgtaa acgaagatgt cgcatttata actgataatg gcaattatat
     7381 tttagattgt aagttaccaa aaggaattga cccatataag ttccatgagt atttaattca
     7441 tctgacaggt gtgtttgaga caggatactt cttagatatg gcagatcaag ttattgtagg
     7501 cactcaagag ggtgttaaaa ttttagaaaa ataataatag tatagaaaag cgcttgctag
     7561 catcatcttc ttaagaggta ctagcaagcg ctttgttatt atttgttttt atctgaagat
     7621 tgttcttcag ttttttcgat atgtttttct gcatcgatta caactgatga acgtctgtct
     7681 tctttataag tcgcaggttc ttttaatgat tgtatttcat cttcatttga atcgtttgtt
     7741 aaatcgtcac tttgtggtgc atgttttact gattctgctg attctactga ctccgaagtg
     7801 atgtcatgat gttcattatg attagattca gtttgattat ctatgtgatg ttgaggttct
     7861 gttttttcag taccttcatg aatgcttggt tcgtcagccg ctgttttttc agtatcattt
     7921 ttagcaatac cgaccgttga tgcagtagct actcctgttt tttcagcatc tttaatattt
     7981 gaagaagatt tttcagcctc agtatgatta ttttcttcat cgtcattgac atggtctaaa
     8041 taattattag gctcaagttc ttcaaggttt cgtcttgttg ttaaatatgc aatacctcgg
     8101 ataattaagc ttaaaccaat gtatcctact gcaacgattg ctgttgaaat ggcgatgact
     8161 ttgattgaaa atagatcgcc gatttcttcg ctaaacaaga aaataagtcc aaatgtcatt
     8221 gaagcatgga atgtcgttgc aatataaatt gtacgtcctt tagtcgctct aattaattca
     8281 ccaagaatca tagagaatgc gaatgtgtaa aggaagttat aagcagcaaa ttctgtacca
     8341 taagttgtat ttgctgagaa aactgaatac atcaaaccaa caacaatact tgcaaagaac
     8401 gtgttcattt tcgtttcaac aatattttgt aagtatgaac ggaatccgaa ttcaactacg
     8461 aacgccatta gaatatgtcc aatcagaatg tgtgtaatag gtactgataa gcctgttgat
     8521 tgtaataaaa taaagctatc tgcaaatgta ttaaagctgt acataccaat aattagaata
     8581 attaaaggta aaattaaagc tagcaataga cgttcaatta ctttcaggct gattgagaat
     8641 tttaaacctg caagttggac ctttttatat ttgaaaacga gtatacaaat gattgcagca
     8701 attaatggtg ctagatctgt aacttcaaat ataaagtgtt tgacaccaat tatagactgg
     8761 aaatctctga gcataatgga taacgccatt gtaatgacaa agaagacgaa aatcgtcatt
     8821 gcccattgaa aaccagaaat tttattgttc ttcattatat gtaacctcca ttaggtaaca
     8881 agcaaaatat cttttagtaa caattataca tattacaagc cgagagataa attgtctatg
     8941 tcaatattaa tgaaaatgta atagtcttga cgtattttat taacataaat cagctaagta
     9001 ttcgttatta catagaaaat aggggtaaaa gaaatgtagt taagtatttt taagatacgg
     9061 atttaactat gaattgaatt caaaatttcc tatacgttat actttaattg ttaataagcc
     9121 acataaataa gagggggaaa tgctgtgtac aagcaaggtg aaccaaattt atggactgga
     9181 aggttagata gtgaaacaga cccgaaaaaa tttagacatt ttcaaacagt aacatttgaa
     9241 gatttatcta agctggaaaa gagtagtagg ccatcagggg tcggtatatt aggctatgct
     9301 gttgacaaag gtgttgcttt aaacaagggg cgcattggtg caaaagaagg accagatgcg
     9361 attaaacaag catttgcagg tttgccggat ttgaatcaat gtgaagcttt agtcgattac
     9421 ggaaatgttt atcatgatta tgaggaatta attgatactc aaaaagaatt tgctatgctt
     9481 gcagcgaagt caattgctaa tcatagacaa acatttttat taggtggggg acatgatatt
     9541 gcgtatgctc aatatttagc aacacgtaaa gtctatccaa cacaatctat tggtgtgata
     9601 aatattgatg cgcattttga tacacgtgct gaacaacaat ctacatctgg aacgagcttt
     9661 agacaaattt tagaagaaga tgaaaacaca gattatttag tgcttggtat tgctcaaggt
     9721 ggtaatacgc aaagtttatt tgattatgct aaagagaaaa agattggtta tgtctttgca
     9781 gatgaattat tgagtcacgt atcaccaaca attaaagata tgattgaacg ttttgtacat
     9841 gaacatgatg tcattatgtt tacgatttgt atggatgtca ttgatagtgc gtttgcgcct
     9901 ggggtaagtg cgccggcagt gttaggtttg tacccacata ctgttcttga attggcaaaa
     9961 cgtattattc caagtgataa ggtgtcttca gttagtattg ctgagatgaa tccaacatat
    10021 gacgctgaca atagaactgc gaagctcgtt gctaatttag tgcatcattt tttaaaataa
    10081 ttaaagatat ttatatgtga cgaaatttgc attgcagggc agaatgtgag tatttcgtaa
    10141 tagaagttat attaacgttg atatatttga aaaataattg cagtaagaag actcttaagt
    10201 tgtagtcatc ttactgctta tttatgtttg ttggtctgaa tgtaatgatt tatacatgga
    10261 aatcataaaa ttggttgtga aattcttata cagttgactg agcaactgca taagggaccc
    10321 ttaataactt aagttaaagg caaaaggtat acagttggct gcgcaacagc ataagaggct
    10381 ctaataactt aagttaaagg caaaaggtat acagttgact gatcaactgc ataagaggct
    10441 ctaataactt aagttaaagg caaaaggtat acagttgact gggcaactgc ataagaggct
    10501 ctaataactt aagttaaagg caaaaggtat acagttgact gagcaacagc ataagaggct
    10561 ctaattactt aagttaaagg caaaaggtat acagttgact gggcaacagc ataagaggct
    10621 ctaataactt aagttaaagg caaaaggtat acagttgact gggcaacagc ataagaggct
    10681 ctaataactt aagttaaagg caaaaggtat acagttgact gcgcaacagc ataagaggct
    10741 ctaattactt aagttaaagg caaaaggtat acagttgact gggcaactgc ataagaggct
    10801 ctaattactt aagttaaaga gcctctaaaa ggccatataa ataatcttgg cattgttgta
    10861 cgaatgtatg tacttgttcg gagtgtttac gctttttatg ataattgata taaatttttc
    10921 ggcctaagtt tggacgaatt tttttatatt ctaaattgga cgtgtgaaat gattggtaat
    10981 aaaatctcgg aatgatagcg taacctaagc caagatgaac aaatccaact gctgattcga
    11041 atcgatctgt ttctacaacg acatttggac gaatatttct tctattaaaa tagtcatcta
    11101 agtgtttgcg cacttgagaa tttttgtttg gcaatatgag tggcaaattt tcaacatcta
    11161 cccaattttg atttttaaat gtttctttag gtgctaataa aatgtaggat tcctcatata
    11221 aaggaatgga tcttatatct tcgtgggtta ttttttcatt tgttatagct aaatgaatat
    11281 taaaattcag taattgctct atagattgat gtttatcatg tatttcatat aaacgatatt
    11341 gctgttcagg gtagtcggaa tggtgctttc gaattaaatt cgcaatccat tgattcgtag
    11401 attcaagagt cccaattttt atccttggtt ctgatgtaac gcttaaatca tacatttttt
    11461 ccatcgtgga tcgatattgt tgaactaatt cgctcgcata acgataaaac tgtattcctt
    11521 tttcggtaat cttgatgtct tttgttgaac gtgtaaataa gtcataacct aaatctgctt
    11581 ccattttttt aatcgtagca gttaaagatg gctggctaat atgtaaaaat tgtgcagctt
    11641 tagtaaaact attatatttc acgatagcca agaaatattc taactgaata attttcatgt
    11701 gttttgctcc ttttatttat agattcaagc tatggataat tagtttattt atatttgttg
    11761 gctatatttt aacatattat aatgaatatt gaagataata atgtaagcgt tttaaattta
    11821 gtggaattaa cttctaaaaa gaacaggagc gaactttatg agaaaaattc aagcaaaaaa
    11881 aggtttgagt attgagtgta aaggttggga acaagaagca gtacttagaa tgttatacaa
    11941 caatttagac ccagaagttg ctgaaagacc agaagattta gttgtttatg gtggtattgg
    12001 aaaagcagct cgtaattggg aagcttttga agcaatagag aaaacgttac gagaattaga
    12061 atcagatgag acaatgttag tacaatcagg aaagccggtt gctgtattta aaacacatga
    12121 agaagcgcca cgtgtattaa tttcaaattc agtattagtg cctgaatggg caaactggga
    12181 tcattttaat gaattagata aaaaaggttt aatcatgtat ggacagatga cagccggtag
    12241 ttggatatat attggttctc aaggtattgt gcaaggtact tatgagacat ttgccgagct
    12301 aggtaatcaa cactttaatg gagatttagc aggcactgtt acactgacag caggtttagg
    12361 tggtatgggt ggtgcgcaac cgttagcaat tactatgaat catggggtag caatttgcgt
    12421 ggatgttgat gaaacacgtg ttgataagcg aattgatacg aaatactgtg atgttaaaac
    12481 agctgattta gatgaagcat taaaattagc agaagaggcg aaagaacgtg gagaaggatt
    12541 atcaatcggg ttagttggaa atgctgtaga catccatcaa gcgattctag aaaaaggatt
    12601 taaaattgac attattactg accaaacaag tgcacatgat ccgctaaatg gatatgtgcc
    12661 acaaggatac tctgtagaag aagcgaaagt attgcgtgaa aaagatccga aaaaatatgt
    12721 tgaactgtca caagcttcaa tggcaaagca tgttgaatta atgcttgaat tccaaaaacg
    12781 tggcgctgta gcatttgatt atggtaacaa tattcgtcaa gtagccttca ataacggagt
    12841 gaaaaatgct tttgacttcc caggttttgt accagcttac attagaccat tattctgtga
    12901 aggtaaaggg ccattccgct ttgctgcgtt gagtggtgat ccaaaagata tcgagcgtgc
    12961 ggatgaagaa atgcgcaaac ttttcccaga aaatgaaaag ttattaagat ggcttgattt
    13021 agctgaagaa aaaatttcat atcaaggact accatcacgt attgcttggt taggctatgg
    13081 agaaagagcg aaaatgggct tagctttaaa tcgtcttgta cgtgacggtg aaatttcagc
    13141 accaattgtt attggacgag accatttaga tgctggttca gttgctagtc ctaaccgtga
    13201 aacagaaagt atgaaagatg gtagtgacgc ggttggtgat tgggctgtat tgaatgcact
    13261 aattaacaca gctgcaggtg gttcatggat ttcattccat catggtggcg gtgttggcat
    13321 gggatattca cttcatgcgg gtatggttgt tgtagcagat ggatcagagc gtgctgaaag
    13381 aagattggaa cgcgtattga ctactgatcc aggtatgggt gttgcccgac atgttgatgc
    13441 tggctatgac atcgctattc aaacagctaa agaaaaaggt attcatattc caatgattga
    13501 taaagcaggt gataagtaat gaatgattta ataattaatc atatagcaga attaatttta
    13561 ccgagatcaa cagataaacc tttgaagggt aaggaattag atgaattaaa tgttgtgaaa
    13621 aatggtacag ttgtcattaa agatggcaaa attgtatatg ctggacaaca tacagatgac
    13681 tacgatgcga ctgagacgat tgatgctagt ggaaaagtag tttctccggc attagtagat
    13741 gctcataccc atttaacatt tggtggatct cgagaacatg agatgtcatt aaaacgtcaa
    13801 ggtaagtctt accttgaaat attagaaatg ggtggtggca tattatctac cgttaatgct
    13861 actagagaaa catccgaaga tgacttgttt aaaaaagcag agcatgattt gcttactatg
    13921 attaaacacg gcgtgcttgc agttgaaagt aagagtggtt atggattaga tagagaaaat
    13981 gaactgaagc aattgaaagt gtctaatcgt ttagctgaga aatatgattt agacatgaaa
    14041 catactttcc tagggcctca tgctgtacct aaagaggcaa gttcaaatga ggcattttta
    14101 gaagaaatga ttgcgttact tccagaagta aaacaatatg cagattttgc ggatattttc
    14161 tgtgaaacag gtgtatttac aatagaacaa tcccaacatt atatgcaaaa agccaaagaa
    14221 gcaggtttta aagtgaaaat acatgcggat gaaattgatc cgttaggcgg actggaatta
    14281 gcaattgatg aacaagcaat atcagcagac cacttagtag cttctagtga taaaggaaaa
    14341 gaaaagttgc gaaatagtga tactgtagct gtcctcttac ctgcaacgac gttctattta
    14401 ggtaaagaag attatgcaga tgcaagaggc atgctagaca ataacggtgc gattgcatta
    14461 gcaactgatt ataaccctgg tagtagtgtc acaaacaact tacaacttgt tatggcgatt
    14521 gcagccttga aattaaagct atcacctaat gaagtttgga atgctgttac ggtcaatgct
    14581 gctaaagcaa tagacattaa cgcggggaca attaacacag gtgataaggc taatttagta
    14641 atttgggatg caccaaatca tgaatatatt ccatatcatt ttggtatcaa tcacgcagaa
    14701 aaagttatta aagacggtaa ggtcatcgta gataacacat tatcttttaa agcataaaat
    14761 aattaga
//
LOCUS       DAHHRR010000060        14562 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.060, whole genome shotgun sequence.
ACCESSION   DAHHRR010000060 DAHHRR010000000
VERSION     DAHHRR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14562
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.060"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            362..736
                     /locus_tag="NBI42_001575"
     CDS             362..736
                     /locus_tag="NBI42_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD1777995.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            751..2397
                     /gene="fakA"
                     /locus_tag="NBI42_001576"
     CDS             751..2397
                     /gene="fakA"
                     /locus_tag="NBI42_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HCD1777996.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAVEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKLDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHSAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            2587..4647
                     /gene="recG"
                     /locus_tag="NBI42_001577"
     CDS             2587..4647
                     /gene="recG"
                     /locus_tag="NBI42_001577"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCD1777997.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRFFFNSQGTQNQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDLDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLI
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEIDVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRRFVEENLLH
                     RSFD"
     gene            4850..5422
                     /gene="fapR"
                     /locus_tag="NBI42_001578"
     CDS             4850..5422
                     /gene="fapR"
                     /locus_tag="NBI42_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCD1777998.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            5427..6413
                     /gene="plsX"
                     /locus_tag="NBI42_001579"
     CDS             5427..6413
                     /gene="plsX"
                     /locus_tag="NBI42_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCD1777999.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYK
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYSLLDQEDTLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKIAGAILKKDLDAFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            6406..7332
                     /gene="fabD"
                     /locus_tag="NBI42_001580"
     CDS             6406..7332
                     /gene="fabD"
                     /locus_tag="NBI42_001580"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCD1778000.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLYNNNDQATEILTSAANTLDFDV
                     LETMFTDEDGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKELIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFLNYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKKNRDVKLTSIQTLEDVKGWNEND"
     gene            7325..8059
                     /gene="fabG"
                     /locus_tag="NBI42_001581"
     CDS             7325..8059
                     /gene="fabG"
                     /locus_tag="NBI42_001581"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCD1778001.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            8493..8726
                     /locus_tag="NBI42_001582"
     CDS             8493..8726
                     /locus_tag="NBI42_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCD1778002.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            8842..9573
                     /locus_tag="NBI42_001583"
     CDS             8842..9573
                     /locus_tag="NBI42_001583"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCD1778003.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            9720..13286
                     /gene="smc"
                     /locus_tag="NBI42_001584"
     CDS             9720..13286
                     /gene="smc"
                     /locus_tag="NBI42_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCD1778004.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLIELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQDQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKNLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIHGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEVNGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETHLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQSQQTKHQLNDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEATLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            13286..14536
                     /gene="ftsY"
                     /locus_tag="NBI42_001585"
     CDS             13286..14536
                     /gene="ftsY"
                     /locus_tag="NBI42_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCD1778005.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDV
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGAK"
BASE COUNT         5498 a         1911 c         2903 g         4250 t
ORIGIN      
        1 aatgaataat tttcacataa aagtcaaaaa tactgagttt tgtgatataa ttgtagactg
       61 tgaagttatg tagtatgata ttttagagaa ataagtgaat gaaacattta aactttttat
      121 ttaatgatac tacatctatt aaaagaaaac gcctatagat aatcgtcatg agtgtaaatt
      181 cagtttactc aaaatataat aggtgattta agcgtaagtc attctgataa gttgtaccat
      241 tgatatttta taaaaataat caatgaaact taaagcgttt atgactacac taagtggtaa
      301 aattttaaaa gtgattaggg aaaacgaata ataaactttg aaatttagga gggacaaaga
      361 tatgacatta gagatttcaa atgattacgg taaaattgat atttcaaacg aagtgattgc
      421 ttcggttgta ggtggaaagg ccgttgaatg ttatggtatt gtaggtatgg catctagaca
      481 acaagttaga gatggtattg cggaaatact aggacatgaa aattatgcta aaggcatcaa
      541 agtaactgaa aataatggcg tagtggatat agatatgtac attattgtaa gttacggcgt
      601 gaaaatatct gaagttgcca ataatgtaca atcaacagtg aaatatactt tggaaaaatc
      661 acttaatgta tcagtaaatt caatcaatat atatgtacaa ggtgtacgtg tgaataatac
      721 aggcaagaaa gcttaggagg acaacttgaa atgattagca aaattaatgg taaattattt
      781 gccgatatga ttatacaagg ggcacaaaat ttatctaaca atgcagattt ggtagattct
      841 ttgaatgtgt atccagtgcc agatggtgat acaggaacaa atatgaatct tactatgact
      901 tcaggtcgcg aagaagtaga gaataatttg tcgaaaaata tcggcgaatt aggtaaaaca
      961 ttctcgaaag gtttactaat gggtgcaaga ggtaactctg gtgtcatctt gtcacaatta
     1021 ttcagaggat tttgtaaaaa tattgaaagt gaatctgaaa ttaattcaaa attgttagct
     1081 gaaagttttc aagctggtgt tgaaacggca tataaagctg ttatgaaacc agttgaaggt
     1141 acaatactta cagttgcaaa agatgctgcg caagctgcag tagaaaaagc aaataatact
     1201 gaagattgta tagaattaat ggagtacatt attgtaaaag ccaatgaatc acttgaaaac
     1261 acaccaaact tattagctgt acttaaagaa gttggtgttg ttgatagtgg tggtaaaggt
     1321 ttgttatgcg tttacgaagg attcttaaaa gcgcttaaag gtgaaaaagt tgaagccaaa
     1381 gttgcaaagc tagataaaga tgaatttgta catgatgaac atgatttcca tggtgtaatt
     1441 aatactgaag atattattta tggctactgt actgaaatga tggttcgttt tggaaagaat
     1501 aaaaaagcct ttgatgaaca agaattcagg caagatatga gtcaatttgg tgattcttta
     1561 ttagtcatta atgatgaaga aattgtgaaa gttcacgtgc ataccgaata cccaggtaaa
     1621 gtgtttaatt atggtcaaca atatggtgaa ttaattaaac ttaaggttga aaatatgaga
     1681 gaacagcacc gtgaagtgat tcgaaaagag cagcactcag ctaaaccgaa aatggaaacg
     1741 gttgaaacag caattattac tatttctatg ggtgaaggta tttcagagat atttaaatca
     1801 atgggtgcca cacatatcat tagtggtgga caaacgatga atccttctac agaagatatc
     1861 gttaaagtca ttgaacaatc aaaatgtaaa cgtgcaatta ttttaccgaa taataaaaat
     1921 atcttaatgg caagtgaaca agcagctagt attgttgatg cagaagcagt tgttattcca
     1981 acgaaatcta ttcctcaagg tataagcgca ctattccaat atgatgtgga cgcaacactt
     2041 gaagaaaata aagcgcaaat ggctgattca gttaataacg ttaaatctgg ttcattaacg
     2101 tacgctgttc gtgatacgaa aattgatggc gttgagatta aaaaagacgc gtttatgggc
     2161 ttgattgaag ataagattgt aagtagccaa agtgatcaat taacaacggt tactgagttg
     2221 ttaaatgaga tgttagcaga agatagtgaa atattgactg tgattattgg ccaagatgca
     2281 gagcaagcag ttacagataa catgataaac tggatcgaag agcaatatcc agatgtagaa
     2341 gtggaagttc atgaaggtgg acaaccaatt tatcaatatt tcttttcagt agaataaaaa
     2401 tttaaaataa aaaactacca atgataaatc atcagttggt agttttttat tttgctattt
     2461 tagtgagata gcgggttaaa agtatcattc tcgagttgct aacaatgtca tgttcaacat
     2521 agtcatgata aaataaataa catactaaat gatacgtaaa atcaaataaa acataggtga
     2581 tttattttgg cgaaagtaaa cttaatagaa agtccatatt ctcttttaca attaaaaggt
     2641 ataggtccta agaaaataga agtattgcaa caactaaata ttcatacagt ggaagatctt
     2701 gttctttatt tgccaactag atatgaagat aatacagtga ttgatttgaa tcaagcagaa
     2761 gatcaatcta acgttacgat agaaggacaa gtatatacag ctccagtagt tgcatttttt
     2821 ggaagaaata aatcaaaatt aactgttcat ttaatggtaa ataatattgc tgtcaaatgt
     2881 atttttttca atcaaccgta tttaaaaaag aaaatcgaat taaatcaaac tataactgtt
     2941 aaaggcaagt ggaatagggt taaacaggaa attactggta ataggttttt ctttaattca
     3001 caaggtacac aaaatcaaga aaacgcagat gttcaattag aaccagtcta tcgtattaag
     3061 gaaggtatta aacaaaagca aatacgagac caaattagac aagcattaaa tgatgtgaca
     3121 attcatgaat ggttaactga tgaactaaga gaaaaatata aattagagac cttggacttt
     3181 actttgaaca cgttacatca tcctaaaagt aaagaggatt tattacgtgc tcgtagaacc
     3241 tatgcattta ctgaactgtt tttattcgaa ttacgtatgc aatggctaaa tagattagaa
     3301 aagtcatctg acgaagcaat tgaaattgat tatgacttag accaagttaa atcatttatt
     3361 gatcgtttac cttttgaact aactgaagca cagaaatcca gtgttaatga aatttttaga
     3421 gatttaaaag caccaatacg aatgcatcga ttacttcaag gtgatgtagg ttcaggaaaa
     3481 acagtagttg ctgcaatttg tatgtatgcg ttaaaaacgg ctggttatca atcagcattg
     3541 atggtaccaa ctgaaatttt agcagagcaa catgctgaaa gtttaattgc tttatttgga
     3601 gattctatga acgttgcgtt gttaactgga tcagtaaaag gtaagaaacg aaaaatactt
     3661 ttagaacaac ttgaaaatgg tacgattgat tgtttaattg gaacccatgc attgattcaa
     3721 gatgatgtga ttttccataa tgttggttta gtaattacag atgaacaaca tcgatttggt
     3781 gtgaatcaac gccagctttt aagagaaaaa ggtgcgatga cgaatgtgtt atttatgaca
     3841 gcaaccccga taccaagaac actagcaata tcagtttttg gtgagatgga tgtgtcttca
     3901 attaaacaat taccaaaagg tcgtaaacct atcattacta cttgggcaaa gcatgagcaa
     3961 tacgataaag ttttgatgca aatgacctca gagttgaaaa aaggtcgaca agcatatgtc
     4021 atttgcccgc ttatagaaag ttctgagcat ctcgaagatg ttcaaaatgt tgtcgcattg
     4081 tacgagtctt tacaacagta ttatggtgtt tcccgtgtag ggttattgca tggtaagttg
     4141 tctgccgatg aaaaagatga ggtcatgcaa aagtttagca atcatgagat agatgtttta
     4201 gtttctacta ctgttgttga agtaggtgtt aatgtaccaa atgctacttt tatgatgatt
     4261 tatgatgctg atcgatttgg actatctact ttgcatcagt tgagaggtcg tgttggaaga
     4321 agcgatcaac aaagttactg tgttttaata gcatcaccaa aaactgaaac tggtatagaa
     4381 agaatgacaa tcatgacaca gacaacagat ggttttgagc tgagtgaacg cgatttagaa
     4441 atgcgtggtc ctggagattt ctttggtgtt aaacaaagtg gattgccaga tttcttagtt
     4501 gccaatttag ttgaagatta tcgtatgtta gaagttgctc gtgatgaagc agctgaactt
     4561 attcaatctg gcgtattctt tgaaaatacg tatcaacatt tacgtcgttt tgttgaagaa
     4621 aatttattac atcgcagttt tgactaattg ccatcctgat tcgtcaattt aagtgcaaca
     4681 cttcgttaat tgagtgatat gacacttgaa ctatttaaat gtaaagtggg attttaaaaa
     4741 tttataaatt tttgactaaa taatagctaa ataatacagt tatttgttga gtcggttaaa
     4801 tagaaagtgt tatgatatgt gaggaatgtt taagactagg tactaaaaaa tgaggggtga
     4861 gacgttgaaa ctaaagaaag ataaacgtag agaagcaatc agacaacaaa ttgatagcaa
     4921 tcccttcatc acagaccatg aactaagcga cttatttcaa gtgagtatac aaacaattcg
     4981 tttagatcgc acttatttaa acataccaga attaaggaag cgtattaaat tagttgctga
     5041 aaagaattat gaccaaataa gttctataga agaacaagaa tttattggtg atttgattca
     5101 agtcaatcca aatgttaaag cgcaatcaat tttagatatt acatcggatt ctgtttttca
     5161 taaaactgga attgcgcgtg gtcatgtgct gtttgctcag gcaaattcat tatgtgttgc
     5221 gctaattaag caaccaacag ttttaactca tgagagtagc attcaattta ttgaaaaagt
     5281 aaaattaaat gatacggtaa gagcagaagc acgagttgta aatcaaactg caaaacatta
     5341 ttacgtcgaa gtaaagtcat atgttaaaca tacattagtt ttcaaaggaa attttaaaat
     5401 gttttatgat aagcgaggat aaaattatgg ttaaattagc aattgatatg atgggtggcg
     5461 acaatgcgcc tgatatcgta ttagaagccg tacaaaaggc tgttgaagac tttaaagatc
     5521 tagaaattat acttttcggt gacgaaaaaa agtataaact gaaccatgaa cgaatcgaat
     5581 ttagacattg ttctgaaaag attgaaatgg aagatgagcc tgttagagcg attaaacgta
     5641 aaaaagatag ttcaatggta aaaatggctg aagctgtgaa atctggtgag gcagatggat
     5701 gtgtgtcagc aggaaatact ggtgctttaa tgtcagctgg tttattcatt gttggacgta
     5761 ttaaaggtgt agctagaccg gctttagtag taacattgcc aacgattgat ggaaaaggtt
     5821 ttgttttttt agacgttggt gcaaatgcag atgctaaacc tgaacactta ttacagtatg
     5881 cgcaactagg ggatatttat gctcaaaaaa ttagaggtat tgataatccg aaaatctcat
     5941 tattaaatat aggtaccgag ccagctaaag gtaatagttt aacgaaaaaa tcttattcgc
     6001 tattagatca agaagacaca ttaaattttg ttggaaacat tgaagcgaag acattaatgg
     6061 atggcgatac tgatgttgta gttacagatg gctatacagg aaacatggta cttaaaaact
     6121 tagaaggtac tgctaaatca attggcaaaa tgttgaaaga tacaattatg agtagtacta
     6181 aaaataaaat agcaggagca attttgaaaa aagatttaga tgcatttgct aaaaaaatgg
     6241 attactcaga atatggtggt tctgtattat taggattgga aggtaccgta gttaaagcac
     6301 acggtagttc aaatgctaaa gctttttatt ctgcaattag acaagcgaaa atcgcaggag
     6361 agcaaaatat tgtacaaaca atgaaagaga ctgtaggtga atcaaatgag taaaacagca
     6421 attatttttc cgggacaagg tgcccaaaaa gttggtatgg cacaagattt atataacaac
     6481 aacgatcaag caactgaaat tttaacttca gcagcaaaca cgttagactt tgatgtttta
     6541 gagacaatgt ttactgacga agacggcaaa ttgggtgaaa ctgaaaacac gcaaccagct
     6601 ttattgacgc atagttcggc attattagca gcactaaaaa atttgaatcc cgattttact
     6661 atggggcata gtttaggtga atattcaagt ttagttgcag ctgacgtatt atcatttgaa
     6721 gatgcagtta aaattgttag aaaacgtggt caattaatgg cgcaagcatt tcctactggt
     6781 gtaggaagca tggctgcagt attgggctta gattttgata aagtcgatga aatttgtaag
     6841 tcattatcat ctgatgacaa aataattgaa ccagcaaaca ttaattgccc aggtcaaatt
     6901 gttgtttcag gtcacaaaga attaattgat gaacttgttg aaaaaggaaa atctttaggc
     6961 gcaaaacgtg taatgcctct tgcagtttca gggccattcc attcttcgtt aatgaaagtt
     7021 attgaagaag actttttaaa ttatattaat caatttgaat ggcgtgatgc taagtttcct
     7081 gtagttcaaa atgtaaatgc gcaaggtgaa actgacaaag aagtaattaa atctaatatg
     7141 gtcaaacaat tatattcacc agtacaattc attaactcaa cagaatggct aatagaccaa
     7201 ggtgttgatc attttattga aattggtcct ggaaaagttt tatctggctt aattaaaaaa
     7261 aaaaatagag atgttaagtt aacatcaatt caaactttag aagatgtgaa aggatggaat
     7321 gaaaatgact aagagtgctt tagtaacagg tgcatcaaga ggaattggac gtagtattgc
     7381 gttacaatta gcagaagaag gatataatgt agcagtaaac tatgcaggca gcaaagagaa
     7441 agctgaagca gtagtcgaag aaatcaaagc taaaggtgtt gacagttttg cgattcaagc
     7501 aaatgttgcc gatgctgatg aagttaaagc aatgattaaa gaagtagtta gccaatttgg
     7561 ttctttagat gttttagtaa ataatgcagg tattactcgc gataatttat taatgcgtat
     7621 gaaagaacaa gaatgggatg atgttattga cacaaactta aaaggtgtat ttaactgtat
     7681 ccaaaaagca acaccacaaa tgttaagaca acgtagtggt gctatcatta atttatcaag
     7741 tgttgttgga gcagtaggta atccgggaca agcaaactat gttgcaacaa aagcaggtgt
     7801 tattggttta actaaatctg cggcgcgtga attagcatct cgtggtatca ctgtaaatgc
     7861 agttgcacct ggttttattg tttctgatat gacagatgct ttaagtgatg agcttaaaga
     7921 acaaatgttg actcaaattc cgttagcacg ttttggtcaa gacacagata ttgctaatac
     7981 agtagcgttc ttagcatcag acaaagcaaa atatattaca ggtcaaacaa tccatgtaaa
     8041 tggtggaatg tacatgtaat atatttgagt taaagctcaa gataaagacg tagtaatcaa
     8101 taaattgata actgataagc ggaacgcctg agctaaagct cattgacgca gtggttgact
     8161 ggtcatccaa tggagaattg cctgacctag tcaactttgc gggggaaatt ctaagcaacc
     8221 aagataaggt tccagaattt ctccctaaga aacagtaatc aataaattga taagtagatt
     8281 agaggaacgc ctgagctaaa gctcaagata aagacgtagt aatcaataaa ttgataactg
     8341 ggatagaggt acgcctgagc taaagctaat gcataagaaa cagtaatcaa taaattgata
     8401 actgtttcta aaatttctac ttgtttttta gaatttaaaa tgggaaaata taatagtcta
     8461 tgtataggca tttttaaagg aggtgaatcg acgtggaaaa tttcgataaa gtaaaagata
     8521 tcatcgttga ccgtttaggt gtagacgctg ataaagtaac tgaagatgca tctttcaaag
     8581 atgatttagg cgctgactca cttgatatcg ctgaattagt aatggaatta gaagacgagt
     8641 ttggtactga aattcctgat gaagaagctg aaaaaatcaa cactgttggt gatgctgtta
     8701 aatttattaa cagtcttgaa aaataataaa tcttacatct gggtcgtcag tattgtcgac
     8761 tcagtttttt tctttaatta tcaatagttt taacgtaaaa ttaaagatga ttcaagagca
     8821 acacataaag gagataaaat aatgtctaaa caaaagaaaa gtgagatagt taatcgtttt
     8881 agaaagcgct ttgatactaa aatgacagag ttaggcttta cttatcaaaa tattgattta
     8941 taccaacaag cattttcgca ttcgagtttt attaatgatt ttaatatgaa tcgtttagac
     9001 cataatgagc gtttagagtt tttgggtgat gcggtattag aattgacggt ttcacgatat
     9061 ttatttgata aacatcccaa cttgccagaa gggaatttaa caaaaatgcg tgccactatt
     9121 gtatgtgagc cctcacttgt aatatttgcg aataaaattg gattgaacga aatgatttta
     9181 cttggtaaag gtgaagaaaa aacaggggga cgtacaagac cgtcattaat atcagatgca
     9241 ttcgaagcat ttattggggc attgtatttg gatcaaggac tagatatagt ttggaaattt
     9301 gctgagaaag tcattttccc acatgtagaa caaaatgagt tattaggcgt ggtagatttt
     9361 aaaacacaat tccaagaata tgtgcaccag caaaataaag gtgatgtaac ctataattta
     9421 ataaaagaag agggaccggc acatcatcgt ctattcactt cagaagttat tctgcaaggg
     9481 gaagcaatag ctgaaggtaa agggaaaacg aaaaaagaat cagaacaacg tgctgctgaa
     9541 agtgcctata agcaattaaa gcaaattaaa tagaaattta tatgatacga catataggtg
     9601 ttcgatttag gatttatgga agaattttta atattttatg gaacaacctc gaaatttaat
     9661 atacaaatac taaaatatcg tatacgtatg ttctttaaaa ttgtgataag gagtttagga
     9721 tggtttattt aaaatcaata gatgccattg gatttaagtc ttttgcagat caaaccaatg
     9781 ttcaattcga taaaggtgta actgcaattg ttggtccaaa tggaagcggt aaaagtaata
     9841 ttacagatgc tattaaatgg gtgttgggtg aacaatcggc taaatcatta cgtggctcaa
     9901 aaatggaaga tattatcttc tcaggtgcag aacatcgcaa agctcaaaat tatgctgaag
     9961 tacagttaag attagataat cattctaaaa agctcagtgt tgatgaaaac gaagttattg
    10021 taacaagaag attgtatcga agtggtgaaa gtgagtacta cataaataat gaccgtgcaa
    10081 gattaaaaga tattgccgat ttatttttag attctggatt ggggaaagaa gcatatagca
    10141 ttatctcgca aggtagagtt gatgaaatac taaatgctaa accaattgat agacgtcaaa
    10201 ttattgaaga atcggctggt gtacttaaat ataaaaaacg taaagctgaa tcattaaata
    10261 aacttgacca aacagaagat aatttaacga gagtagaaga cattttatat gatttggaag
    10321 gtcgcgtaga acctctaaaa gaggaggcag ctatagctaa agaatataag acactttcac
    10381 accaaatgaa acatagtgac attgtagtta cagtgcacga tattgatcaa tatacaaatg
    10441 acaatagaca attagatcaa cgtttaaatg atttacaagg tcaacaagca aataaagaag
    10501 ctgacaagca acgtttaagc caacaaattc aacaatataa aggtaaacgt catcaacttg
    10561 ataatgatgt tgaatcgctt aattatcaat tagtaaaagc tacggaagcc tttgaaaaat
    10621 atacgggaca attaaatgtt ttagaagaac gtaagaaaaa tcaatctgaa acaaatgcac
    10681 gatatgaaga agaacaagaa aatttaattg agcttttaga aaatatatca aatgagattt
    10741 ctgaagctca agatacttat aagtctctga aaagtaaaca aaaagaactc aacgctgtca
    10801 ttcgtgaatt agaagaacaa ctatatgttt cagacgaagc acacgatgaa aaattggaag
    10861 aaattaaaaa cgaatactat acattaatgt cagagcaatc agatgttaac aatgatattc
    10921 gttttttaaa gcatactata gaagagaatg aggctaaaaa atcaagacta gattctcgat
    10981 tagttgaagt ctttgagcaa ttgaaagata ttcaggatca aataaaaacg actaaaaaag
    11041 aatatcaaca gaccaacaaa gaactttctg ctgtagataa agaaattaaa aatatagaaa
    11101 aaaacctcac tgatacaaaa aaagcacaaa acgaatacga agagaaattg tatcaagcat
    11161 atcgatatac cgaaaaaatg aaaacacgta ttgatagttt ggcaacgcaa gaggaagaat
    11221 atacttattt tttcaatggc gtcaaacata ttttgaaagc taaaaataaa gaattaaagg
    11281 gtattcatgg tgcagttgca gaaattattg atgtgccatc taaattaact caggcaattg
    11341 aaacagcatt aggtgcttca ttacaacatg tcattgtaga ttcagaaaaa gatggacgcc
    11401 aggctattca atttttaaaa gaacgtaatt taggtcgtgc gacgttttta ccattaaatg
    11461 ttatacagag tagagtggta gcgactgata ttaagtctat tgctaaagag gtaaacggat
    11521 ttattagtat tgcttcggaa gcagttaaag tagcaccaga atatcaaaat attatcggga
    11581 atttattagg taatacgatt atcgttgatc atttaaagca tgcaaatgaa ttggcacgag
    11641 ctattaaata tcgaactcgt attgttactt tggaaggtga tattgtaaat cctggtggtt
    11701 ctatgactgg tggtggcgct cgtaagtcaa aaagtattct gtctcaaaaa gacgagttga
    11761 caacaatgag acaccaatta gaagattact tgcgtcaaac agaatcattt gaacaacaat
    11821 ttaaagagtt gaagataaaa agtgatcaat taagtgaact gtattttgaa aaaagtcaaa
    11881 agcataatac acttaaagag caagtgcatc attttgaaat ggagctcgat agattaacta
    11941 cacaagaaac acaaataaaa aatgatcatg aagaattcga atttgaaaaa aatgatggtt
    12001 atacgagtga caaaagtcga caaactttga gtgaaaaaga aactcattta gaaagtatta
    12061 aagcatcttt aaaacgacta gaagatgaaa ttgaacgcta cacaaaactt tctaaagaag
    12121 gtaaggaaag cgtaactaaa acacaacaaa cgttacatca gaaacaatct gatcttgctg
    12181 tggttaaaga gcgtattaaa acacaacaac agacaataga tcgattaaat aatcaaagtc
    12241 aacaaaccaa acatcaatta aatgatgtta aagaaaaaat agcattcttt aattcggatg
    12301 aagtgatggg cgaacaagct tttcaaaata ttaaagatca aattaatggt caacaagaaa
    12361 cgagaacacg cttatctgat gaattagata aattgaaaca acaacgtatt gagttgaatg
    12421 aacaaatcga tgcgcaagaa gctacactac aagtttgtca ccaagatatt ttagctatcg
    12481 aaaatcacta ccaagatatt aaagctgaac aatcaaagct agatgtatta attcatcatg
    12541 cgatagatca tttaaatgat gaatatcaat tgactgttga acgtgcaaaa tctgaatata
    12601 cgagtgatga atcgattgac gcattacgta aaaaagttaa attaatgaag atgtcgattg
    12661 atgaactagg tcctgtaaac ttaaatgcaa ttgaacaatt tgaagagtta aatgaacgtt
    12721 atacattttt aagtgagcaa cgtacagatc ttcgtaaagc taaagaaaca ttagagcaaa
    12781 ttataagtga aatggatcaa gaggttactg aaagatttaa agaaactttc catgctattc
    12841 aaggacattt tacagctgtg ttcaaacaat tgtttggtgg aggcgatgca gaattgcaat
    12901 taactgaagc cgattattta acagctggta ttgatattgt ggtacaacca ccgggtaaaa
    12961 agttgcaaca tttatcgtta ctaagtggtg gtgagcgtgc attaactgct attgctttac
    13021 tatttgcaat tttaaaagta agatctgcac cttttgttat attagatgag gttgaagctg
    13081 cattagatga agcgaatgtt attagatacg caaaatattt aaatgagtta tcagacgaaa
    13141 cacaattcat tgttattaca caccgtaaag gaacaatgga atttgcagat aggttatacg
    13201 gtgtaacaat gcaagaatca ggtgttacta aacttgtgag tgtgaattta aatacaatag
    13261 atgatgtgtt gaaggaggag caataatgag cttttttaaa cgcttaaaag ataagtttgc
    13321 aacaaataaa gaaaatgaag aagttaaatc cttaacagaa gaacaaggtc aagacaaatt
    13381 agaagataca cattctgaag gttcaacgca ggacgtaaat gatttagcag aaaatgctga
    13441 agtgaaaaag aagccacgca agttgagtga agcggatttt gatgacgatg gcttaatatc
    13501 aattgaagat tttgaagaaa ttgaagctca aaaaatgggt gctaaattta aagcaggact
    13561 cgaaaaatct cgtcaaaatt tccaagaaca attaaataat ttgatagcga gatatcgtaa
    13621 agtagatgaa gacttttttg aagctttaga agaaatgtta atcactgcag acgtcggttt
    13681 taatacagtg atgacgttaa ctgaagaatt acgtatggaa gcacaacgac gtaatattca
    13741 agatactgaa gatttgcgtg aagtcattgt tgaaaaaatc gtagagattt accatcaaga
    13801 agatgataat tcagaagcta tgaacttaga agatggtcgt ttaaatgtca ttttaatggt
    13861 cggtgtgaat ggtgttggta aaacaacaac aattggaaaa ttagcttacc gatataaaat
    13921 ggaaggtaaa aaagtaatgt tagctgcggg cgatactttt agagcgggtg ctattgatca
    13981 attgaaagtt tggggcgaac gtgttggtgt agatgtaatt agccaaagtg aaggttctga
    14041 tccagctgct gttatgtatg atgcgattaa tgccgctaaa aacaaaggtg ttgatatttt
    14101 aatctgtgat accgctggac gtttacaaaa taaaacaaat ctaatgcaag aattagaaaa
    14161 agttaagcgt gtaattaatc gagcagtgcc agatgcgcct catgaagcat tactatgttt
    14221 agatgctaca actggtcaga atgcgttgtc acaagctaga aactttaaag aagtaacaaa
    14281 tgttacaggt attgtattaa cgaaattaga tggtacagcc aaaggtggta tcgtattagc
    14341 cattcgtaat gaattgcaca ttccagttaa atatgtaggt ttaggtgagc aattagatga
    14401 cttacaacca tttaaccctg aaagttatgt ctacggctta tttgctgata tgattgaaca
    14461 aaatgaagaa ataacaacag ttgaaaatga tcaaattgta acagaagaaa aggacgataa
    14521 tcatggggca aaatgattta gttaaaacgt tacgaatgaa tt
//
LOCUS       DAHHRR010000061        14494 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.061, whole genome shotgun sequence.
ACCESSION   DAHHRR010000061 DAHHRR010000000
VERSION     DAHHRR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14494
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.061"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            746..2041
                     /gene="purB"
                     /locus_tag="NBI42_001586"
     CDS             746..2041
                     /gene="purB"
                     /locus_tag="NBI42_001586"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCD1778006.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIEVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            2150..2452
                     /locus_tag="NBI42_001587"
     CDS             2150..2452
                     /locus_tag="NBI42_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778007.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            2624..3316
                     /locus_tag="NBI42_001588"
     CDS             2624..3316
                     /locus_tag="NBI42_001588"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HCD1778008.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVAFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYM
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            3313..5505
                     /gene="pcrA"
                     /locus_tag="NBI42_001589"
     CDS             3313..5505
                     /gene="pcrA"
                     /locus_tag="NBI42_001589"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCD1778009.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVSSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            5509..7512
                     /gene="ligA"
                     /locus_tag="NBI42_001590"
     CDS             5509..7512
                     /gene="ligA"
                     /locus_tag="NBI42_001590"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCD1778010.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNESNS"
     gene            7525..8724
                     /locus_tag="NBI42_001591"
     CDS             7525..8724
                     /locus_tag="NBI42_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HCD1778011.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGPTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8813..10351)
                     /gene="putP"
                     /locus_tag="NBI42_001592"
     CDS             complement(8813..10351)
                     /gene="putP"
                     /locus_tag="NBI42_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCD1778012.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            10717..11019
                     /gene="gatC"
                     /locus_tag="NBI42_001593"
     CDS             10717..11019
                     /gene="gatC"
                     /locus_tag="NBI42_001593"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCD1778013.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            11021..12478
                     /gene="gatA"
                     /locus_tag="NBI42_001594"
     CDS             11021..12478
                     /gene="gatA"
                     /locus_tag="NBI42_001594"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCD1778014.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            12491..13918
                     /gene="gatB"
                     /locus_tag="NBI42_001595"
     CDS             12491..13918
                     /gene="gatB"
                     /locus_tag="NBI42_001595"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCD1778015.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            13958..14104
                     /locus_tag="NBI42_001596"
     CDS             13958..14104
                     /locus_tag="NBI42_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778016.1"
                     /translation="MIADLDAIEGLFISIEVILLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
BASE COUNT         5443 a         2096 c         2679 g         4276 t
ORIGIN      
        1 atgtaataat ttagtagctt atttactaaa ttgcaaacat ctcattcaat aattaatatg
       61 atcgaaagct tcatgaaaat atattagttg atttcgaaag aagcgtagtc aatttatgtc
      121 ttgctataaa aatttactat taagaaaatc tctgcgtatt tttctataaa cagtttcgta
      181 tttatacgat tctggagttc gatattaatg gcttatattt aaaatgctac acctgtgcaa
      241 tagaacatat attctttttg tgagtagaag atttgttata ttagaagtga ataattttaa
      301 aacaaattat gaaatgtaag aagaaatatt atgaaactag gtaaagtaga ttgttgaaaa
      361 ttccatacaa taggagaagt aaagtgaata ttatctggta ttactaaagc ttttgattgt
      421 cctaattgta ataaaaagtt ggatagtgta ggtgagggga gaaaaactaa aatgcaataa
      481 tatattctta atggatcatt ataaatgcca attagattaa agtgttgcga acatttacaa
      541 ttagaaatca gtattaatct gccatagata attgtattaa tgtaaaaaat aaaatagaat
      601 gagaatatac ggaaattttt tatacattta taaccaattc tgttaaactg tgaagagtag
      661 agttaaaaac gaacttttta tttattttca ttaaaaatgt tctgtttttg aaaattcgag
      721 tatacatacg ataggagtta aaaaaatgat tgaacgctat tctagagaag aaatgtctaa
      781 tatttggact gatcaaaatc gctatgaagc atggttagaa gtggaaattt tagcatgtga
      841 ggcatggagt gaattaggac atataccgaa agctgacgta caaaaaattc gtcaaaacgc
      901 aaaggtaaat gtcgaacgtg cacaagaaat tgaacaagaa acgcgccatg atgttgtagc
      961 ctttactaga caagtttctg aaacgctagg tgaagaacgt aagtgggtac attatggttt
     1021 aacttctact gatgttgtag atacagcttt aagtttcgtt attaaacaag caaatgacat
     1081 tattgaaaaa gatttagaaa gatttatcga ggttttagct gaaaaagcaa aaaattataa
     1141 atatacattg atgatgggac gtacacatgg agtgcatgca gaaccaacta catttggtgt
     1201 caaaatggca ttgtggtaca cggaaatgca acgtaactta caacgtttca aacaagtaag
     1261 agaagaaatt gaagtaggga aaatgagtgg tgcagtaggt acttttgcta acattcctcc
     1321 tgaaatagaa agttatgtgt gtaagcattt aggtattggc acagcgcctg tatcaacgca
     1381 aacattgcaa cgcgatcgac atgcatacta tattgcgaca ttagcattaa tcgcgacgtc
     1441 actagaaaaa tttgccgttg aaattcgtaa ccttcaaaaa accgaaacaa gagaagttga
     1501 agaagcattt gcaaaaggtc aaaaaggttc atctgcaatg cctcataaac gaaatccaat
     1561 tggttctgaa aatatcacag gtatttcaag agtaattcgt ggttatatta ctacagctta
     1621 tgagaatgtg ccattatggc atgaaagaga tatttctcat tcttcagctg agcgtattat
     1681 gttaccagat gttacaatag cactagatta tgcattgaat cgtttcacga atattgtgga
     1741 ccgtttaact gtatttgaag ataatatgcg taataacata gataaaacat ttggtctgat
     1801 tttctcacaa cgtgtcttat tagcattaat taataaaggt atggttcgtg aagaagcata
     1861 cgacaaagta caaccaaaag cgatgatatc ttgggaaacg aaaacacctt tccgtgaatt
     1921 aatcgaacaa gatgagtcta ttacaagtgt tttaacaaaa gaagaattgg atgaatgttt
     1981 cgatcctaaa catcatttga atcaagttga cactattttc gaacgcgctg gattagcata
     2041 aaattaaata aagtaacgat tcagatttaa taaaatataa atcgctacac tttaatagat
     2101 taatgcgtta aaatgacata aagaataaaa ttataggggt tgtttcgata tgcaaattga
     2161 aaaattacga ggtgcagcgt tagatgaatt gtttgatgca atactaacgt tagaaaatag
     2221 agaagaatgt taccaatttt tcgatgattt gtgtactgta aatgaaattc aatcactgtc
     2281 tcaaagatta caagttgcta aaatgattaa gcaaggttat acctatgcaa cgattgaaca
     2341 agaatctgga gcatcgactg caacgatttc tagagtgaag cgttcattac aatggggtaa
     2401 tgatgcttat acaatgattt tagatcgtat gaatattgaa acaaatgaat aataaatgaa
     2461 cgataaacaa tggttatcta tctgcactaa taaggtagat aatcattgtt ttttcacgaa
     2521 aaaatttaca gagtaaaaga acttaaattt catattaagt ctttagaact cgacacttaa
     2581 aaatgctata atcatatgta tgttaaaaaa ggagtttcgg aaaatgtatg acattaaaaa
     2641 atggcgccat atttttaaat tagacccagc taaacatatt tcagatgatg atttagatgc
     2701 gatttgtatg tctcaaacag atgcaattat gattggtgga actgatgacg ttactgaaga
     2761 taatgtcatt catttaatga gcagggtaag aagataccca ttgcctttag tacttgaaat
     2821 atcaaacatc gaaagtgtaa tgcctggttt tgatttttat tttgtaccta cagtactgaa
     2881 cagtacagat gttgcatttc acaatggtac attattagaa gcgcttaaaa catatggaca
     2941 tagtatagat tttgaagaag taatatttga agggtatgtc gtgtgcaatg ctgatagcaa
     3001 agtggcaaaa cataccaaag caaatacaga tttaacaaca gaagatttag aagcatatgc
     3061 ccaaatggtc aatcatatgt atcgattacc ggttatgtat atggagtata gtggcattta
     3121 tggcgacgta tcaaaggttc aagctgtctc agaacatcta acagaaacgc aactttttta
     3181 tggtggcggt atttcctcag aacaacaagc gacagagatg gcagctattg cagatacaat
     3241 tatcgtcggt gatattattt ataaagatat taaaaaagct ttaaaaacag taaaaataaa
     3301 ggagtctagt aaatgaatgc gttattaaat catatgaata cagagcaaag tgaagctgta
     3361 aagacaacag aaggaccatt gttaattatg gcaggtgctg gttcagggaa gacacgtgtt
     3421 ttaacacata gaattgctta tttattagac gaaaaagatg tctcaccata caatgttttg
     3481 gctattactt ttacaaataa agctgcaaga gaaatgaaag aacgtgttca aaaattagta
     3541 ggtgatcaag cagaagttat ttggatgtca acattccact caatgtgtgt tcgtatttta
     3601 cgtcgtgatg cagatcgaat tggtatagaa cgcaatttta cgataattga tcctacagac
     3661 caaaaatctg ttattaaaga cgtcttaaaa aatgaaaata ttgatagtaa aaagtttgaa
     3721 cctcgtatgt ttatcggtgc aatcagtaat ttgaaaaatg aacttaaaac acctgcagat
     3781 gctcaaaaag aagccacaga ttatcactcg caaatggtag caactgttta tagtggatat
     3841 caacgacaat tgtcacgtaa tgaagcgtta gattttgatg accttattat gacaacgatt
     3901 aacttatttg agcgtgtacc agaagttcta gaatattatc agaacaaatt ccaatatatt
     3961 catgtagatg agtatcaaga tactaataaa gcacaatata cattagttaa attattagca
     4021 agtaagttta aaaacttatg tgttgtaggt gactcagatc agtcaattta tggttggcgt
     4081 ggtgctgata ttcaaaatat cttatcattt gaaaaagact acccagaagc gaatacaatc
     4141 tttttagagc aaaattatcg ttcgacgaaa acgattttaa atgcggctaa cgaagtgatt
     4201 aaaaataatt ctgaacgtaa gccaaaagga ctgtggactg caaatacgaa tggtgaaaaa
     4261 attcattact atgaagcaat gacagaacgt gatgaagcgg aatttgtaat acgagaaatt
     4321 atgaagcatc aacgtaatgg taagaaatat caagatatgg caattttata tagaacaaat
     4381 gcacaatcac gtgtacttga ggaaacattc atgaaatcta atatgccata cacaatggtt
     4441 ggtggccaaa agttctatga ccgtaaagaa atcaaagatt tattaagtta tttgcgtata
     4501 attgctaata gtaatgatga cattagtttg caacgtatta ttaatgttcc aaaaagaggt
     4561 gtaggtcctt catctgttga aaaagttcaa aattatgcac ttcaaaacaa tatcagtatg
     4621 tttgatgcac ttggagaagc tgattttatc ggattgtcaa aaaaggtgac acaagagtgt
     4681 cttaattttt acgagttgat ccaaagcttg ataaaagaac aagaattttt agaaattcat
     4741 gaaatagttg atgaagtatt acaaaagtct ggctaccgag aaatgcttga acgtgaaaat
     4801 acactagaat ctcgaagtag attagaaaac atcgatgaat ttatgtcagt accaaaagac
     4861 tatgaggaaa ataccccatt agaagaacag tcattaatta actttttaac ggatttatcg
     4921 ttagtagctg atattgatga ggcagatact gaaaatggcg taacactaat gacgatgcac
     4981 tcggctaagg gccttgaatt tccaatcgtc tttataatgg ggatggaaga atctttattc
     5041 ccacatatta gagcgattaa gagtgaagat gatcatgaaa tgcaagaaga acgtcgtatt
     5101 tgttatgtag caatcacaag ggctgaagag gtgttatata ttactcatgc gacatcaaga
     5161 atgttatttg gtcgccctca gtcaaatatg ccatctaggt ttttaaagga aattccagaa
     5221 tcactattag aaaatcattc aagtggcaaa cgacaaacga tacaacctaa ggcaaaacct
     5281 tttgctaaac gtggatttag tcaacgaaca acgtcaacga aaaaacaagt atcttcatct
     5341 gattggaatg taggtgacaa agtgatgcat aaagcctggg gagaaggcat ggtgagtaat
     5401 gtaaacgaga aaaatggctc aatagaacta gatattatct ttaaatcaca agggccaaaa
     5461 cgtttgttag cgcaatttgc accaattgaa aaaaaggagg attaagggat ggctgattta
     5521 tcgtctcgtg tgaacgagtt acatgattta ctaaatcaat acagttatga atactatgta
     5581 gaggataatc catccgtacc agatagtgaa tatgacaaat tacttcatga actgattaaa
     5641 atagaagagg agcatcctga gtataagact gtagattctc caacagttag agttggcggt
     5701 gaagcccaag cctctttcaa taaagtcaac catgacacgc caatgttaag tttagggaat
     5761 gcatttaatg aggatgattt gagaaaattt gaccaacgta tacgtgaaca aattggcaac
     5821 gttgaatata tgtgcgaatt aaaaattgat ggcttagcag tatcattgaa atatgttgat
     5881 ggatacttcg ttcaaggttt aacacgtggt gatggaacaa caggtgaaga tattaccgaa
     5941 aatttaaaaa caattcatgc gatacctttg aaaatgaaag aaccattaaa tgtagaagtt
     6001 cgtggtgaag catatatgcc gagacgttca tttttacgat taaatgaaga aaaagaaaaa
     6061 aatgatgagc agttatttgc aaatccaaga aacgctgctg cgggatcatt aagacagtta
     6121 gattctaaat taacggcaaa acgaaagcta agcgtattta tatatagtgt caatgatttc
     6181 actgatttca atgcgcgttc gcaaagtgaa gcattagatg agttagataa attaggtttt
     6241 acaacgaata aaaatagagc gcgtgtaaat aatatcgatg gtgttttaga gtatattgaa
     6301 aaatggacaa gccaaagaga gtcattacct tatgatattg atgggattgt tattaaggtt
     6361 aatgatttag atcaacagga tgagatggga ttcacacaaa aatctcctag atgggccatt
     6421 gcttataaat ttccagctga ggaagtagta actaaattat tagatattga attaagtatt
     6481 ggacgtacag gtgtagtcac acctactgct attttagaac cagtaaaagt agctggtaca
     6541 actgtatcaa gagcatcttt gcacaatgaa gatttaattc atgacagaga tattcgaatt
     6601 ggtgatagtg ttgtagtgaa aaaagcaggt gacatcatac ctgaagttgt acgtagtatt
     6661 ccagaacgta gacctgaaga tgctgtcaca tatcatatgc caacccattg tccaagttgt
     6721 ggacatgaat tagtacgtat tgaaggcgaa gtagcacttc gttgcattaa tccaaaatgc
     6781 caagcacaac ttgttgaagg attgattcac tttgtatcaa gacaagccat gaatattgat
     6841 ggtttaggta ctaaaattat tcaacagctt tatcaaagcg aattaattaa agatgttgct
     6901 gatattttct atttaacaga agaagattta ttacctttag atagaatggg gcagaaaaaa
     6961 gttgataatt tattagctgc cattcaacaa gctaaggaca actctttaga aaatttatta
     7021 tttggtctag gtattaggca tttaggtgtt aaagcgagcc aagtgttagc agaaaaatat
     7081 gaaacgatag atcgattact aacggtaact gaagcggaat tagtagaaat tcatgatata
     7141 ggcgataaag tagcacaatc tgtagttact tacttagaaa atgaagatat tcgtgcttta
     7201 attcaaaaat taaaagataa acatgttaat atgatttata aaggtatcaa aacatcagat
     7261 attgaaggac atcctgaatt tagtggtaaa acgatagtac taactggtaa gctacatcaa
     7321 atgacacgca atgaagcatc taaatggctt gcatcacaag gtgctaaagt tacaagtagc
     7381 gttactaaaa atacagatgt cgttattgct ggtgaagatg caggttcaaa attaacaaaa
     7441 gcacaaagtt taggtattga aatttggaca gagcaacaat ttgtagataa gcaaaatgaa
     7501 tcaaatagtt agaggggtat gtcgatgaag cgtacattag tattattgat tacagctatc
     7561 tttatactcg ctgcttgtgg taaccataag gatgatcagg ctggaaaaga taatcaaaaa
     7621 cataacaata gttcaaatca agtaaaagaa attgctacgg ataaaaatgt acaaggtgat
     7681 aactatcgta cattgttacc atttaaagaa agccaggcaa gaggactttt acaagataac
     7741 atggcaaata gttataatgg cggcgacttt gaagatggtt tgttgaactt aagtaaagaa
     7801 gtgtttccaa cagacaaata tttgtatcaa gatggtcaat ttttggataa gaaaacaatt
     7861 aatgcctatt taaatcctaa gtatacaaaa cgtgaaatcg ataaaatgtc tgaaaaagat
     7921 aaaaaagaca agaaagcgaa tgaaaattta ggacttaatc catcacatga aggtgaaaca
     7981 gatcctgaaa agattgcaga aaaatcacca gcctatttat ctaacatttt agagcaagat
     8041 ttttatggtg gtggagatac aaaaggtaag aatattaaag gtatgacgat tggtttagct
     8101 atgaatagtg tttattacta taaaaaagaa aaagatggac cgacttttag taaaaaacta
     8161 gatgatagcg aagttaaaaa gcaaggtaaa caaatggcta gtgagatatt atcaaggtta
     8221 cgtgaaaatg atgatttaaa agatatacca attcattttg caatttataa gcaatcaagt
     8281 gaagattcaa tcacaccagg tgaatttatc actcaagcga ctgcagaaaa gagtcaaaca
     8341 aagcttaatg aatggcataa tatcaatgaa aaatcagctt tattaccttc ttcaacagca
     8401 gcagattatg atgaaaattt aaataataat ttcaagcaat ttaacgataa tttgcaatca
     8461 tatttttcta atttcacaca agcagtaggg aaagttaaat ttgttgataa aaagccacaa
     8521 cgattagtag tagatttacc aatcgattac tatggacaag ctgaaacaat tggtattaca
     8581 cagtacgtta ctgaacaagc gaataaatat ttcgataaaa tcgataacta tgaaattcgt
     8641 attaaagatg gtaaccaacc acgtgcttta attagtaaga caaaagatga caaagaaccg
     8701 caagttcata tttacagtaa ttaattgtaa attaaagata ataaaaaggt accaatgtta
     8761 gtcagaattt tgattaacat tagtaccttt ttaatttgtt aagacttatg aattattttt
     8821 ctctaacgat gtcgcggact ttgtttaagt cagtttcaac aaatgcacca ggttttttag
     8881 taagtttact tacaacatat gtaacgatta cacttacaat aaatccagga ataatttcat
     8941 ataagccgaa tatttcgttg atatgtgcca atggtttaat ccacgcaatc caaacgataa
     9001 cgactaaggc acctgaaacc attccactta cagcaccggc acgtgtcaaa cctttccagt
     9061 aaagtgcaaa tagcacaagt ggactgaatg atgcaccaaa tccggcccaa gcattaccta
     9121 ctaagtttag aattgtgtcg tttggattcc atgcaatagc gatggcaaca attgctacaa
     9181 ctaatacaga taatcttcca atcataacaa attctttttg gtgcgtttta gctttttctt
     9241 caccacgaat taatttataa aagtcttccg ttaatgaact agatgttaca agtaattgtg
     9301 aagaaatcgt gctcataatt gcagctagaa tcgcagcaag taagaaacca cctacaagag
     9361 gatggaagag tacttgactc atcacgatga ataatgtctc aggatcttct agtttaatat
     9421 gataagcagg tacgaatgca atacctgtta aaccaacagc cacagcgcct aataaaccaa
     9481 ctgccatcca gctaatacct aaacgtctag ctttaggtag catcttgtgt gatttaatag
     9541 acataaacct tacaatgata tgaggttgac cgaaataacc taatccccat gaaaatagag
     9601 agataattcc tataaatgat aaccctttaa ataaatttaa atttgtaggt ttcatagctg
     9661 ctacatcatg aaacgttccc cagccgttta aattcatcat agcaacaata gggaccataa
     9721 ccatcgcaat taacataatg acaccttgga agaaatctgt aattgataca gctaaatatc
     9781 caccaaagaa agtatagaaa atgacaatga aagcaactaa tattaaaccg aaatgataat
     9841 ctaatccaaa agcactttca aataatttac caccagatac gaaaccagaa tgtgtatata
     9901 atgtaaagaa tactacgata atcaatccag aaataatctt taacacatta tttttatcgt
     9961 ttaaacgatt tttaaagaaa tctggtaatg taattgcatc tccagctaat tcggtataaa
    10021 cacgaagtct aggagcaaca acaaagtaat ttatataagc acctaatgtt aaaccgattg
    10081 taatccacat agctgataga ccagtgctat agacagaacc aggtagaccc ataatcatcc
    10141 atccactcat atctgaagct ccagctgata atgcagtaat atacggtcca atactacgtc
    10201 cacctaacat gtactcgctt aagttaccag ttgcttgctt gtaaccataa aagccaataa
    10261 cgattagtat caagaagtag acggcaatca taatataagt ttgccaattg gcatctactt
    10321 gttgacttaa tgctgtcccc attgtaagca tgataaacac tccctttatt taatttataa
    10381 tttagttact tgaaaacatt atacataaag ataaatgttt agaaaagtag gaaaattaaa
    10441 tttattcttt ttgaaatact tttataccaa tatagaaagc gatttcgtat tttttggata
    10501 tgggcaaatt tttataatta aaattgcata atcataatat tgaattgggt tgtctaatat
    10561 taaagggtgt tgttttcata aaaaataatt tggtaaacga atgataatgt ttatacagtt
    10621 tatatgaaat taaagtagca cctcataaat acttagattt ttaattggaa atttgataca
    10681 atttagtgat gaatgactta aaggaggctt ttattaatga caaaagtaac acgtgaagaa
    10741 gttgagcata tcgcgaatct tgcaagactt caaatttctc ctgaagaaac ggaagaaatg
    10801 gccaacacat tagaaagcat tttagatttt gcaaaacaaa atgatagcgc tgatacagaa
    10861 ggcgttgaac ctacatatca cgttttagat ttacaaaacg ttttacgtga agataaagca
    10921 attaaaggta ttccgcaaga attagctttg aaaaatgcca aagaaacaga agatggacaa
    10981 tttaaagtgc ctacaatcat gaatgaggag gacgcgtaag atgagcattc gctacgaatc
    11041 ggttgagaat ttattaactt taataaaaga caaaaaaatc aaaccatctg atgttgttaa
    11101 agatatatat gatgcaattg aagagacaga tcccacaatc aagtcatttt tagcacttga
    11161 taaagaaaat gcaattaaaa aagcgcaaga attggatgaa ttacaagcaa aagaccaaat
    11221 ggatggcaaa ttatttggta ttccaatggg tataaaagat aacattatta caaacggatt
    11281 agaaacaaca tgtgcaagta aaatgttaga aggatttgtg ccaatttacg aatctactgt
    11341 aatggaaaaa ctacataatg agaatgccgt tttaattggt aaattaaata tggatgagtt
    11401 tgcaatgggt ggttcaacag aaacatctta tttcaaaaaa acagttaacc catttgacca
    11461 taaagcagta ccaggtggtt catcaggtgg atctgcagca gcagttgcag ctggcttagt
    11521 accatttagc ttaggttcag acacaggtgg ttcaattaga caaccggctg catattgtgg
    11581 cgttgtcggt atgaaaccaa catacggtcg tgtatctcga tttggattag ttgcttttgc
    11641 atcttcatta gaccaaattg gtccattgac tcgaaatgta aaagataatg caatcgtctt
    11701 agaagctatt tctggtgcag atgttaatga ctctacaagt gcacctgttg atgatgtaga
    11761 ctttacatct gaaattggta aagatattaa aggattaaaa gttgcattac ctaaagaata
    11821 cttaggtgaa ggtgtagctg atgacgtaaa agaagcagtt caaaacgctg tagaaacttt
    11881 aaaatcttta ggtgctgtcg ttgaggaagt atcattgcca aatactaaat ttggtattcc
    11941 atcatattac gtgattgcat catcagaagc ttcgtcaaac ctatctcgtt ttgacggtat
    12001 tcgttatggt tatcattcta aagaagctca ttcattagaa gaattatata aaatgtcaag
    12061 atctgaaggt ttcggtaaag aagtaaaacg tcgtattttc ttaggtacat ttgcattaag
    12121 ttcaggttac tacgatgctt actataaaaa atctcaaaaa gttagaacat tgattaaaaa
    12181 tgactttgat aaagtattcg aaaattatga tgtagtagtt ggtccaacag cgcctacaac
    12241 tgcgtttaat ttaggtgaag aaattgatga tccattaaca atgtatgcca atgatttatt
    12301 aacaacacca gtaaacttag ctggattacc tggtatttct gttccttgtg gacaatcaaa
    12361 tggccgacca atcggtttac agttcattgg taaaccattc gatgaaaaaa cgttatatcg
    12421 tgtcgcttat caatatgaaa cacaatacaa tttacatgac gtttatgaaa aattataagg
    12481 agtggaaatc atgcattttg aaacagttat aggacttgaa gttcacgtag agttaaaaac
    12541 ggactcaaaa atgttttctc catcaccagc gcattttgga gcagaaccta actcaaatac
    12601 aaatgttatc gacttagcat atccaggtgt cttaccagtt gttaataagc gtgcagtaga
    12661 ctgggcaatg cgtgctgcaa tggcactaaa tatggaaatc gcaacagaat ctaagtttga
    12721 ccgtaagaac tatttctatc cagataatcc aaaggcatat caaatttctc aatttgatca
    12781 accaattggt gaaaatggat atatcgatat cgaagtcgac ggtgaaacaa aacgaatcgg
    12841 tattactcgt cttcacatgg aagaagatgc tggtaagtca acacataaag gtgagtattc
    12901 attagttgac ttgaaccgtc aaggtacacc gctaattgaa atcgtatctg aaccagatat
    12961 tcgttcacct aaagaagcat atgcatattt agaaaaatta cgttcaatta ttcaatacac
    13021 tggtgtatca gacgttaaga tggaagaagg atctttacgt tgtgatgcta acatctcttt
    13081 gcgtccatat ggtcaagaaa aatttggtac taaagccgaa ttgaaaaact taaactcatt
    13141 taactatgta cgtaaaggtt tagaatatga agaaaaacgc caagaagaag aattgttaaa
    13201 tggtggagaa atcggacaag aaacacgtcg atttgatgaa tctacaggta aaacaatttt
    13261 aatgcgtgtt aaagaaggtt ctgatgatta ccgttacttc ccagagcctg acattgtacc
    13321 tttatatatt gatgatgctt ggaaagagcg tgttcgtcag acaattcctg aattaccaga
    13381 tgagcgtaaa gctaagtatg taaatgaatt aggtttacct gcatacgatg cacacgtatt
    13441 aacattgact aaagaaatgt cagatttctt tgaatcaaca attgaacacg gtgcagatgt
    13501 taaattaaca tctaactggt taatgggtgg cgtaaacgaa tatttaaata aaaatcaagt
    13561 agaattatta gatactaaat taacaccaga aaatttagca ggtatgatta aacttatcga
    13621 agacggaaca atgagcagta aaattgcgaa gaaagtcttc ccagagttag cagctaaagg
    13681 tggtaatgct aaacagatta tggaagataa tggcttagtt caaatttctg atgaagcaac
    13741 acttctaaaa tttgtaaatg aagcattaga caataatgaa caatcagttg aagattacaa
    13801 aaatggtaaa ggcaaagcta tgggcttctt agttggtcaa attatgaaag cgtctaaagg
    13861 tcaagctaat ccacaattag taaatcaact tttaaaacaa gaattagata aaagataatt
    13921 ttaatcatca aactatgaag atttaaaaaa taaacccttg attgctgact tagatgcaat
    13981 cgagggttta tttatatcta tagaagtcat attactttta actttattca ttgtacatgt
    14041 taatggtaaa aatattaatt ttattaatgc gttagcttta attatattaa ggcaaactgt
    14101 ataataaaaa ggtataaaac atttttgtat aaagacaaca ttatatttac aacatcattt
    14161 taaaggtaaa atagcataac tgacgaagtc tatataacga agaacggcaa aaaatgctga
    14221 ataaataaca acctttgtac atattgagat agtatttgtt taagatacaa gttggtcttt
    14281 aacgatatta agaatgatga aataagactg agcctgggtc ataaattcaa tgtcctaggc
    14341 actacaatgt tatattggca gtagttgact gaaagaaaat acgcttgtaa caagcttttt
    14401 tcaattatag tcgggcccca acattgagaa tttcgaaagg aaattctaca gacaatgcaa
    14461 gttggggtgg ggccccaaca tagaagctga cttt
//
LOCUS       DAHHRR010000062        14275 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.062, whole genome shotgun sequence.
ACCESSION   DAHHRR010000062 DAHHRR010000000
VERSION     DAHHRR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14275
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.062"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            317..1165
                     /locus_tag="NBI42_001597"
     CDS             317..1165
                     /locus_tag="NBI42_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCD1778017.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREATKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            1194..1748
                     /locus_tag="NBI42_001598"
     CDS             1194..1748
                     /locus_tag="NBI42_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HCD1778018.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMIYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            1801..3513
                     /locus_tag="NBI42_001599"
     CDS             1801..3513
                     /locus_tag="NBI42_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1778019.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTRGNITGELYINNQDATVGCLHERSNVVGTVLQDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETMRLLNNIHEETKSTMIVVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLREVCMS
                     EHVKFKVKKWIDKTSSNDDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNKGFNQLSISERSEFVAYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKTCEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            3510..4343
                     /locus_tag="NBI42_001600"
     CDS             3510..4343
                     /locus_tag="NBI42_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD1778020.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLFKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELI
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGMNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIMLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     Y"
     gene            4390..4887
                     /locus_tag="NBI42_001601"
     CDS             4390..4887
                     /locus_tag="NBI42_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1778021.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDYSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNHRSINLAERLNFKRDDTMDDIISQGD
                     IVFYK"
     gene            5005..6129
                     /locus_tag="NBI42_001602"
     CDS             5005..6129
                     /locus_tag="NBI42_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCD1778022.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHLFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDSKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYNYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRQFFKGNRDYEE"
     gene            6433..7251
                     /locus_tag="NBI42_001603"
     CDS             6433..7251
                     /locus_tag="NBI42_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCD1778023.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELNIWQQWLHT
                     QGYNDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            7269..7883
                     /locus_tag="NBI42_001604"
     CDS             7269..7883
                     /locus_tag="NBI42_001604"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCD1778024.1"
                     /translation="MLRIAIAKGRLMDSLINYLDAIEFTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGIADIGIVGSDILDERHYNVNNLLDMPFGACHFAVAAKPET
                     TKYHKIATSYVHTAEAYFKSKGIDVELIKLNGSVELACIVDMVDGIVDIVQTGTTLEA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            7876..9126
                     /locus_tag="NBI42_001605"
     CDS             7876..9126
                     /locus_tag="NBI42_001605"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCD1778025.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEKSV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVNQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDDTADLDAIVYDVFAQAEHDELARTYVISEDAQVLKDLESRIAKVL
                     PNVDRYDIVSKSIANQHYLIHASNFVEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            9142..10155
                     /locus_tag="NBI42_001606"
     CDS             9142..10155
                     /locus_tag="NBI42_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HCD1778026.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKDAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGMVYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIQRIQKIEHPYPLN
                     VFTLNIATYIFRHREETSRFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            10124..10702
                     /gene="hisB"
                     /locus_tag="NBI42_001607"
     CDS             10124..10702
                     /gene="hisB"
                     /locus_tag="NBI42_001607"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCD1778027.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNATLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            10699..11277
                     /gene="hisH"
                     /locus_tag="NBI42_001608"
     CDS             10699..11277
                     /gene="hisH"
                     /locus_tag="NBI42_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCD1778028.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNNPKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLDAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTKYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGTDIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFVND"
     gene            11270..11974
                     /locus_tag="NBI42_001609"
     CDS             11270..11974
                     /locus_tag="NBI42_001609"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCD1778029.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVKQLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGRLVKTTTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            11971..12729
                     /gene="hisF"
                     /locus_tag="NBI42_001610"
     CDS             11971..12729
                     /gene="hisF"
                     /locus_tag="NBI42_001610"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCD1778030.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASCLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKTHYCCTHGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFEQ
                     TDVSAGLAASILHDRETTVQSIKAVIRQGGITVR"
     gene            12726..13358
                     /locus_tag="NBI42_001611"
     CDS             12726..13358
                     /locus_tag="NBI42_001611"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCD1778031.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQHVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQGSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALDVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            14010..14192
                     /locus_tag="NBI42_001612"
                     /pseudo
     CDS             14010..14192
                     /locus_tag="NBI42_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5085 a         2007 c         2766 g         4417 t
ORIGIN      
        1 cgataaataa aagatgcaag ctttttattt cgaattcgaa tttttttatt acataatgtc
       61 caaaagtaat atttataagg cgctacatta gcgttatacc ctagtctgct gatttaaaca
      121 caattaagta attataattc tatgaaatct atgcaacatg ataaatctga ctattattat
      181 catttttata gtcactacta ttgactttgc atcaagtgac tcgtactatt ttaatataat
      241 tctgaaaatt caaaatatag aactttaaat atgtagataa tatttttttg gaagttttaa
      301 aaaaaggagg cattttatgg gaaattattt ggtattgcaa tcagattttg gacttagtga
      361 tggcgctgta agtgcaatgt atggagttgc atatactgta agtgatgata tacgtgtcga
      421 aaacttaacg catgatatac caccgtatga catttgggta gcatcatatc gtttatatca
      481 aactgtaaaa tactggccta aaggcacagt ttttgtatca gtggtagacc cgggtgtagg
      541 tagtgatagg cggagcattg cttgcttaac atattcaggt cattacatta ttacacctga
      601 taatggttct ttaagtcata ttaaacatta cgaaggtatt aaaaaagtta tcgaaattga
      661 tgaagtgaaa agccgattgc ctcattcgga agaaagtcat acttttcatg gtagagatgt
      721 ctatgcatac aatggtgcga gattagctgc aaataagata gcgtttgaac gtctcggtca
      781 ggcaattaat gttgatagta ttgaggcatt agaaataaga gaagcgacaa aaaatgagga
      841 ttctgttaca ggaagcattg atattttaga tatcagattt gggtcattat ggacaaatat
      901 tccattggca ttcttaaaat ccttggaaat tgttcatggt aataatttgg tggtaacaat
      961 ttatcatcaa gataagaaag tctatcaaaa tattattaaa tttgcgcgat cgtttgcaga
     1021 tgttaatgtg ggtgaacctc ttgtgtatgt taactcgcta gtaaatattg gtgtagcggt
     1081 caatcaagac tcattttcag atttatatca tattggtact ggaaatgatt ggacgattca
     1141 tttatctaaa gcaccatcaa tttaatgaat tttttatgta aggagtaatg aagatgaaaa
     1201 aacaagatat ttcagttaaa actgttgttg cgattggtat aggagcagcg gtatttgtta
     1261 ttttagggcg ttttgttgta ataccaacag gttttcctaa tacgaatata gaaacatctt
     1321 atgcattttt agcattaata tctgcaatat ttgggccttt tgctggttta atgactggat
     1381 tagttggaca cgctattaaa gatttcacga catacggtag tgcttggtgg agttgggtta
     1441 tttgttcagg aattataggc tgtttatatg gatggattgg cctaaaatta aatctttctt
     1501 caggccgatt ttctaggaaa tcgatgattt attttaatat cggtcaaatt attgcgaata
     1561 ttatttgctg ggcacttatt gcaccaacat tagatatttt gatttataac gaaccagcta
     1621 acaaggttta tacacaaggt gttatctctg cagtattaaa tattatttct gttggtatta
     1681 ttgggacaat attattaaaa gcatatgctt catctcaaat taaaaaaggt agtttacgta
     1741 aggaatagtt attttgttga atcagatatg taaatgaatg tagaaaggta atgatatatc
     1801 atgactgaac caattatctc gttcaaagac tttagttttc aatatcatag tcaagcaaca
     1861 cctacattac agaatataaa tgttgatatt tatccaggag aaaaagtgct agtagttggt
     1921 gcttcaggta gtggtaaatc gacttttgca aattgcataa acggattaat tccatttaaa
     1981 actagaggta acataactgg ggaactatat ataaataatc aagatgcaac tgttggttgt
     2041 ttacatgaaa gatctaatgt tgttggtaca gttttacaag atacagatgg acagttcata
     2101 ggcttaacag cagctgaaga tatggccttt ttattagaaa ataattgtgt tgaacaagat
     2161 gatatgaaga aaaatgtaag ttattgggct gaaaaagttg gcatgataga acatttaaat
     2221 caccgaccgc aagatttatc tggaggtcaa aaacaacgcg tttcattagg tggtatatta
     2281 atccatcgta cccctatttt aatattggat gagccactag ccaatttaga tcctgcgaca
     2341 ggacatgaaa cgatgagatt actaaataat attcatgaag aaacaaagtc aacgatgatt
     2401 gttgtagaac atcgtttaga agaatcatta gatgatacat ttgatagagt tctccttttt
     2461 aaagatggga aaatcatcgc taatacaacg cctagtgatt tactaaaatc atctaagctt
     2521 aaagaagcag gcatacgtga accattatac tgtacagcgc ttaaatatgc agaagtagat
     2581 gttgaatcca ttgataactt agcgaattta cgtgaagttt gtatgagtga acatgttaaa
     2641 tttaaagtga aaaaatggat agataaaaca tcttcaaatg atgataataa gtacaaatct
     2701 gaaccgctat tagaattaaa tgaggtgtgt gttcaatata gtgactatag taatagtgta
     2761 cttaataatg ttcaattaaa tgtttataga agagaaatgc ttagtatagt tggtcacaat
     2821 ggtgccggca aatcaacact tgctaaagca atttgtggat ttttagatat aacaggtaat
     2881 atccaatttt gtaataaggg atttaatcaa ttgtcaatta gcgaacgatc tgaattcgta
     2941 gcttatgtga tgcaaaatcc aaatcatatg atttccgaaa aaatgattta cgatgaagta
     3001 gcattagggt taagagcacg aggtatgaaa gaatcagata taaagatacg agtagagaat
     3061 gtgctcaaaa tatgcggact ttatgcattt cggaattggc caattgcagc tttaagctat
     3121 ggtcaaaaaa agagggtcac tatagcatct gttttagtct taaatccgga aataatcata
     3181 ttggatgaac cgactgctgg tcaagatttc tatcattata atgagataat gtcattttta
     3241 attgagctaa acagacaggg gaagacgatt attatgatta cgcatgatat gcatttattg
     3301 tctgagtata gttcaagaac agttgtatta tcaaaaggtc aagtcgttgc tgataccacg
     3361 ccagtattgg ttttaaatga taaaaaaacc tgtgagattg catcattgag acaaacatcg
     3421 ctatttgaaa tggccgaata tatagggatt agcgagccac agaaattagt acaattattt
     3481 attaaccatg ataggaaggt gagacgccaa tgaatcaata taatactata ggttttcacc
     3541 cgggaaatag tcgtattcat caattaaatg cgactgttaa acttttattt ttattagttg
     3601 tttctatttc tgcaatggtg acttatgaca caagatattt aattttaatt agtgcttcat
     3661 ctattttatt gttcaaatat gctcatattg aatggaaaca agttcgtttt gttgttaaat
     3721 tcattctgtt tttcacaata ttaaatatta ttgccgtata catatttgat cctgaatatg
     3781 gtgtgaagat ttataatcag cgtacagagt taatcaatgg tattggtcga tttacgctaa
     3841 catcacagga attattctat ctttttaatc taatattaaa atatattagt acagttcctt
     3901 tagcgttaat atttttattc acaacgaatc cgagtcattt tgctgcaagt ttaaatcagc
     3961 taggtatgaa ttataaaatc agttacgcag tctcactagc attaaggtat attccagata
     4021 ttcaagaaac atattttaat atttcacaag cgcaacaagc aagaggatat gataattcga
     4081 aaaaggcgaa atttactagc cgtgttaaag gtatcaaacg cattatgtta cctttaatat
     4141 tttctagtat cgaaagaatt gacactatta gtactgctat ggagttaaga caattcggac
     4201 agtataaaag gagaacctgg tacgtcaaaa aacaattaaa aaaagatgat tatgttgttt
     4261 tgtgtttgac gttaatactt ctgatgttag tagttacatt attcttttta aataatagtc
     4321 gatatttcaa cccgtggtat tagcattcat ataaatagtc tttaaataga aataggaggg
     4381 agacatttaa tgataaatac tgaaagatta aatttaatga ttccaagttc ctcgcattta
     4441 attgaacttt ataatatttg tagtcatcca caagcaaata tatacactcc caaaggttta
     4501 cataattcca aattagacac acaacggtgg attgaaaaat ggcgaaacca ttggcaacaa
     4561 tatcaatttg gttactttgt attggtaaaa aaaatagatt atagtgttat tggtatttgt
     4621 ggatatgaat atcgacaatt aaagcaagaa acagtactaa atttatttta taaattacat
     4681 ccaagttttg aaggacaagg gtatgcatgt gaggctatta cagcaatcac aaattttgtg
     4741 aattatatcg atcaagaaac agtaaaagtt atcaggacaa ataagtgtaa tcatcgttca
     4801 ataaatttag cagaaaggct taatttcaag cgagacgata caatggacga cattatcagt
     4861 caaggagata ttgtgtttta taaataaaat actatgacat tatctaaaaa ataaaattta
     4921 aatcccaaca gtttcttaaa aagttatgag cgacatacta aaaatgttat aattgtgcat
     4981 aataaaacat aaggagaatg tttaatgcat aaaaaatatt tattgctatt ttttctgtta
     5041 ttatcagttg cattgttata tagctgttcc aagcaaaatg agaaagctca aaataaatct
     5101 gttaatccaa atgagtatgc aaagcataaa tcgaaaatgg atgaacattg ggaaacatat
     5161 cacggccaag tatatcattt gttttatcat ccattaatca cagacccaaa agttgcgttt
     5221 acaggagata cacaccaagc taagggcaac aatgattgga tgattactgt aagtgaattt
     5281 aaaaaatcat tgaacgaatt atataaaaat aattacatca tcgttaatcc acatgatgta
     5341 tacgacttat catctcatca tgtaaaagcc aaaaaaataa aattgccgaa aggtaaaaag
     5401 ccactaatca tctcaattga tgatatgaat tactatagtt acatgagaca ccatggttat
     5461 gccgaccgct tagttcttga taaaaataag catgttgtat ctgaaacgac agactcaaaa
     5521 ggccatgcta cttattctga tgacaatgat atcgtgccaa ttttaaataa atttgtaaaa
     5581 gagcatccag atttttcttt aaatggtgaa aaaggtgttg ttgctttaac cggttatgag
     5641 ggcgtattag gttatcgcac gaatgaatta acgagtaaag attatacaaa gaataaaaag
     5701 gcagctgagg aagttgttcg cgcaatgaaa cgggatggat ggtcatttgc gagtcattca
     5761 tatggtcaca ttaattttga aaaaacatct ttagaaggta ttaaacgtga tactaaaaga
     5821 tggaaagatg aagttgaacc aattgtaggt aaaacagata tgtttgtatt tccacatggc
     5881 gcacaggata gacatacaca agcgtataat tatttggtag atgaagcaga gtttaagttt
     5941 attgcaggtg tcggtccaaa caattttaca gatatatcag caacgaacgt ttaccaagat
     6001 agggttgcga ttgatggtct aaacttattt gaatttaaat ataaactcaa accgtttttt
     6061 aatcctgaaa atgtatatag taaacaagat agacaattct ttaaaggtaa tcgggattat
     6121 gaagaatagt taaatttaat aaaaagatag aaagaaaata actttacata ataaggcaag
     6181 gacaatatgt gcaagaagta caacttcaat ataaaaatct tggttaagca tttttatagg
     6241 cacaaatgtc ttgccttttt cttttgaaaa aatttaaaac caatacaccg aattttctga
     6301 aaaaggttgt aatgtattgc taaaaatgat aagttattaa gcatattaca ctttagtatg
     6361 gtagaggagt aaagcggacg acaaaaatac caagtaattt taaatacagt attgaaagta
     6421 aagggtggtc gagtgaataa ttcagaacaa ttaattgcgt taaaagaatc ggaaacagca
     6481 tttttaaaat attttaataa agcggattat gagttagttg attttagtgt ggttgaaaag
     6541 ttggactgga aacaattgaa ccatgaggat ttgcagcaga tgggtgagcg aaatttttgg
     6601 caacatgagc atcaaattta tgcacttcga aatgatttta cagatcaatt attgcgttat
     6661 tactcaatgt atccaactgc agcaacaaaa gtggcataca caggtttaat cattcggaat
     6721 aatgaagcgg ctgtacaagt tggtttggaa aactatgcac catcattagc gaatgtacaa
     6781 cagagcttaa agctatttat tcagtttatt caacagcagt tgagagacaa cgtgcatttt
     6841 gttgtattgg gacattatca attgcttgat gccttactcg ataaatcgtt acaaacacca
     6901 gacatactat caatgattga ggaacgtaat ttatcaggac tggttacata tttgtcgact
     6961 gaacatccaa tcgtgcaaat cttgaaagag aatacgcaac aacaattaaa tgtattggag
     7021 cactatattc caaatgacca tccagcactt gttgaactaa atatttggca acaatggtta
     7081 catacgcaag ggtacaatga tatccattta gatattactg cgcagccacc tagatcctat
     7141 tacacaggtt tatttattca atgtcatttt gctgaaaatg aatctcgagt tttaacaggt
     7201 ggttattaca aaggaagcat cgaagggttt ggattaggat taacacttta agtaagggag
     7261 tatgcactat gttaagaatc gccattgcaa aagggcgtct aatggatagt ttaattaact
     7321 atttagatgc aattgaattt acgacattat cagaaacatt aaaaaataga gaacgccaat
     7381 tattattaag tgtagataat atagaatgca ttttagtaaa agggagtgac gtgccaatct
     7441 atgtggaaca aggaattgca gatattggta tcgttggtag cgacatttta gatgagcgtc
     7501 attacaatgt taataatttg ttggatatgc cttttggagc atgtcatttt gcggtcgcag
     7561 cgaaacccga aacgacaaaa tatcataaaa tcgcaacgag ttatgtccat actgccgaag
     7621 catattttaa atcaaaaggc attgatgtcg aattgattaa attgaatggt tctgttgaat
     7681 tagcttgtat tgtcgatatg gtagacggaa ttgtcgacat cgttcaaaca ggtactactc
     7741 tagaagcgaa cggactggtt gaaaagcaac atattagtga tatcaatgca agattaataa
     7801 ctaataaagc agcttatttt aaaaaatcac aattaataga gcaatttatt cgctctttgg
     7861 aggtgtctat tgccaatgct taatgcacaa caatttttaa atcaattttc attagaagca
     7921 ccattagatg agtcattgta tccaattatt cgcgatattt gtcaggaagt taaagttcat
     7981 ggagataagg ctttgaaaat gtataatcta acattcgatc atacgaaaac agatcattta
     8041 gaaattagtc atgaacaaat taaagcagca tttgacacat tagatgaaaa aacaaaacaa
     8101 gcattacaac aaagttatga aagaattaaa gcatatcaag aaagtattaa gcagacgaat
     8161 caacagttag aaaaatcagt ggagtgttat gaaatatatc atccactaga aagtgtgggt
     8221 atttatgtgc ctggtggcaa agcaagttat ccatcaacgg ttctaatgac agcgacttta
     8281 gcacaagtag cgggtgtaga aaatattgtt gttgtgacac cacctcaacc taacggagta
     8341 tcccaagagg tattagctgc atgttatatt acgcaagtta atcaagtgtt tcaagttggt
     8401 ggtgctcaaa gtattgctgc attgacttat ggaacagaaa caatacctaa agttgataag
     8461 attgtaggtc caggtaacca atttgttgca tatgccaaaa aatatttatt tggacaggta
     8521 ggtattgatc aaattgcagg accaacagaa atagcactga ttattgatga caccgcagat
     8581 ttagatgcca tcgtatatga tgtatttgcg caagcagaac atgatgaatt agcacgtaca
     8641 tatgtcatta gtgaagatgc gcaagtcctt aaagatttag aatcacgtat tgctaaagta
     8701 ttgcctaatg tggacagata cgacattgtt tctaaaagta tcgctaatca acactacctt
     8761 atccatgcta gtaattttgt tgaagcatgc catgtcatga atacaatcgc gcctgaacat
     8821 gcgtcgattc aaacagtaaa tcctcaacca tatattgaaa aagtgaaata tgtgggcgca
     8881 ttgtttattg gacattattc gccagaggtc ataggagatt acgttgcagg tccaagtcat
     8941 gtattaccta caaatagaac agctagattt accaatgggt tatcggtcaa tgatttctta
     9001 acacggaaca cggtcatcca tctatcaaaa gatacgtttg aacaaattgc tgattcagca
     9061 caacatattg cccatgttga agcattatac aatcaccaac agtctatttt aatacgtcag
     9121 tcttagggga gtgtaattga aatgatttat attgataaaa atgaaagccc agttacgccg
     9181 ttggatgaaa aaacaatgac gtctattatt agtgcaacgc catataattt atatcctgat
     9241 gcagcatatg aacaattcaa ggacgcttat gctaagtttt acggattatc gcctgaacaa
     9301 attattgcag gaaatggctc tgatgaattg attcagaagt taatgctgat aatgccagaa
     9361 ggtccggcat taacgctaaa tccagatttt tttatgtatc aagcatatgc ggcacaagta
     9421 aatcgtgaaa ttgcatttgt agatgcagga tcagatttaa cgtttgattt ggaaaccatt
     9481 ttaacaaaaa tcgatgaagt acaaccatca ttttttatta tgagtaatcc acataaccca
     9541 tcaggcaagc aatttgatac ggcattttta acagctattg cagataagat gaaagcatta
     9601 aacggatact ttgtcattga tgaagcatat ttagattatg gcatggtata tgacgtggaa
     9661 ctggcaccac acattttaag aatgcgcaca ttatcaaagg cgtttggaat agctggctta
     9721 agattaggtg tcttaattag tactgctgga acgatacagc gtattcaaaa aatagaacat
     9781 ccatatccat taaatgtatt tacgctaaat attgcgactt atatttttag acatagagaa
     9841 gagacaagtc gatttttaac aatgcaacga cagttagctg agcagttaaa acaaatattt
     9901 gatacacatg ttgcagataa aatgtcagta ttcccatcaa atgccaattt tgtacttact
     9961 aaaggttcag cggcgcaaca attaggacaa tacgtatatg aacaaggatt taaacctcgt
    10021 ttttatgatg agccggtgat gaagggctat gtaagatatt caattgcaac agcatcacag
    10081 ttaaagcaat tagaagaaat tgttaaagaa tggagtgcaa aatatgattt atcaaaaaca
    10141 acgaaacaca gctgaaacac aactaaatat ttcaatatca gatgaccagt caccatcgca
    10201 tattaataca ggtgtgggct ttttaaatca tatgttaacc ttgtttacat ttcatagcgg
    10261 tctgtcatta aacattgagg cacaaggtga tattgacgta gatgatcacc atgtaaccga
    10321 agatatcggc attgtcattg gccaattgtt acttgaaatg attaaagata aaaagcattt
    10381 cgttcgttat ggaacgatgt acattccaat ggatgaaaca ttagcacgtg tcgttgtgga
    10441 tataagtggg cgtccatact tatctttcaa tgcaacgtta agtaaagaaa aagttggtac
    10501 gtttgatacg gaattagtag aagaattttt tagagcagtc gtaatcaatg caagattaac
    10561 aacgcatatt gatttaattc gtggaggtaa tacacaccat gaaattgaag ctatattcaa
    10621 agcattttcc cgtgcattag gcatagcgct aactgcaact gatgatcagc gtgtgccgtc
    10681 atcgaaaggt gtgattgaat gatagtcatc gttgattatg gattagggaa tattagtaat
    10741 gtaaaacgcg ctattgaaca tttggggtat gaggtggttg tctcaaataa cccaaaaata
    10801 atcgatcaag cagaaacaat catattgccc ggtgtcggtc attttaaaga tgcgatgtca
    10861 gagataaagc gattaaatct cgatgcaata ttggctaaga atactgataa gaaaatgatc
    10921 ggtatttgtt taggcatgca attaatgtat gagcatagtg atgaaggcga tgcatctgga
    10981 ttagggttta tcccaggaaa tatttcgcgt atccaaacaa aatacccagt gccgcactta
    11041 ggttggaata atttagtgag taagcatcct atgttaaatc aagatgttta cttcgtacat
    11101 tcttaccaag cgccgatgtc agaaaatgtc attgcatatg cgcagtatgg tactgatatt
    11161 ccagcaattg tccaatttaa caattatatt ggtattcaat tccatcccga aaaaagcggt
    11221 acatatgggc tacaaatttt gcgtcaggca atacaagggg gatttgtaaa tgattgaatt
    11281 atggccagca attgatttga ttgggtcaac tagtgttagg ctaacagaag gtaaatatga
    11341 cagtgaagaa aaaatgtcac gttcggctga agaaagtatt gcttactata gtcaatttga
    11401 atgtgtgaat cgtattcata ttgtcgactt gataggtgct aaggcacagc atgcccgaga
    11461 gtttgattat attaagtcat tgaggagatt aacaaccaaa gatattgaag taggtggtgg
    11521 cattcgtacg aagtcacaaa tcatggacta ctttgccgca ggaattaatt attgcatagt
    11581 tggaacgaaa ggtattcaag atactgattg gcttaaagag atggcacata catttccagg
    11641 tcgcatttat ttatctgttg atgcttatgg cgaggatatt aaagtgaacg ggtgggaaga
    11701 ggacacagag ttgaatttat ttagttttgt gaaacagtta tcggatatac ctcttggagg
    11761 cattatttat actgatatcg ctaaagatgg caaaatgtcc ggacctaact ttgaattaac
    11821 tggtcgatta gtaaagacaa cgacgattcc tgtcatcgcg tctggtggta ttagacatca
    11881 gcaagatatt caacgattag cgtcgctaaa tgttcacgct gctattatag gaaaggctgc
    11941 acatcaagca tcattttggg aggggctaaa atgattaaaa aacgtatcat tccatgttta
    12001 gatgtcaaag atggtcgtgt cgttaaaggg attcaattta aaggattaag ggatattggg
    12061 aatcctgttg atttagcaat gtattacaat gaagcgggtg ctgatgagtt agtattttta
    12121 gacatctcta agacggaaga ggggcatagc ttaatgttag aagtgattga acagacagcg
    12181 tcatgcttgt ttatccctct tactgtagga ggtgggattc aaagtctcga tgatattacc
    12241 caattgttaa atcatggtgc agataaagta tcattaaatt caagtgcttt aaaaaatcca
    12301 cagctaatta aacaagcaag tgataaattt ggtagacaat gtatctgcat agcaattgat
    12361 agctactatg atcctgaaag aaaaacacat tattgttgta cgcatggtgg taaaaagatg
    12421 acaaatatta aagtatatga ctgggtacag caagtagaac agttaggtgc aggtgaattg
    12481 ctcgttacaa gtatggggca tgatggtatg aagcaaggat ttgatattga acatctagcg
    12541 aaaataaagt ctctcgtaaa cattccaatc attgcatctg gtggcggtgg caatgcacaa
    12601 cactttgtag aattgtttga acagacggat gtttctgcag gtttagctgc aagtatattg
    12661 catgacaggg aaacgacggt tcaatctatt aaagcagtga tacgacaagg gggtataaca
    12721 gtaagatgac caaatataaa attgatttta gcaaaggttt agtgccagca attttacaag
    12781 ataatcaaac aaaacaagta ttgatgttgg gttatatgaa tcaagaagct tttgataaaa
    12841 cgatagaaga tggtgtggta tgtttctatt cacgatcgaa acaacgtcta tggactaaag
    12901 gtgaaacatc tggtcatacg caacatgtta aagatattca tgtagattgc gacaatgaca
    12961 ctattttaat tgatgtcata ccaaatggac caacatgtca tacaggcagt caaagttgtt
    13021 tcaatacaga agttccattt tcagtgcaaa cattagctca gacagttcaa ggtagtgccc
    13081 aatccaataa tgaaaagtca tatacaaaat atttattaac agaaggtata gaaaagatta
    13141 caaaaaaata cggtgaagaa gcttttgaag tcgtaattga agcgattaaa ggtgacaaaa
    13201 aagcatttgt aagtgaagtg gcagatgaac tttatcattt atttgtcttg atgcatgcac
    13261 tagacgtcga tttttcagaa attgaggcag aattagcgcg tagacatcat aaacgcaata
    13321 actttaaagg cgagcgacaa aatatcgaac agtggtaaaa caagtatgga ctgagatata
    13381 gagaaaaggt taatggtata taaacttaca aatattgtgg aaaacgtgac attttcaagt
    13441 ttaaaatacg acaccaacat attttaacta tgaatgctgt gatggtacta aagttgcgaa
    13501 cttgttatag ataagtagtg gataatcaca atatgaaacc aaaaataatt tgaaaagtaa
    13561 atatttataa attttaacat tatttaaaat ttgttataat ttgaatagac aaaatattag
    13621 gaggctaatt catgttaatt taatcgagat tatttaaagg gttaggtggt attttggtag
    13681 catctagtat ggtgatttca ccagtaactg taagtgaaaa cacagctaat gctgagaata
    13741 ataagtttga tactaatcaa aaggattata aaattaatca aaatgtagct aacataattt
    13801 ataacaatct tgagaaaaat agtaacaatg agttgaagat tattgataaa aataatttga
    13861 aaagtgaatt gagcaactca ggaatagatg tcactgttaa agatgttgaa aagtatatga
    13921 atcgatataa tgaagttatg aagggaaaaa atggcgaaaa agctaaagag aatttagtag
    13981 cctttaataa agaagtagaa aaacaacaga aaaaaaatac tacaagcaat agagatgctt
    14041 gtgatgctct aactgtaact ggttttgcac atacaactgc tacaacggga gcgatggctg
    14101 ctctaggtgt tagtggtcct attggctggg gtgtaggtac tggaatggca gcactttatg
    14161 ctggcggatc tattgcttgt aataatatgt aggtgaaaaa tatgaaaaaa ttattcaatg
    14221 aatatttttt tactacgagt caagcaactg cattaattat tttttcatta ccaat
//
LOCUS       DAHHRR010000063        14242 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.063, whole genome shotgun sequence.
ACCESSION   DAHHRR010000063 DAHHRR010000000
VERSION     DAHHRR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14242
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.063"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(232..738)
                     /locus_tag="NBI42_001613"
     CDS             complement(232..738)
                     /locus_tag="NBI42_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778032.1"
                     /translation="MKKLLTASIIACSVVMGVGIANTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(874..1674)
                     /locus_tag="NBI42_001614"
     CDS             complement(874..1674)
                     /locus_tag="NBI42_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1778033.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLMLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTASFINKHHDEKHV"
     gene            complement(1658..2377)
                     /locus_tag="NBI42_001615"
     CDS             complement(1658..2377)
                     /locus_tag="NBI42_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1778034.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            complement(2432..3382)
                     /locus_tag="NBI42_001616"
     CDS             complement(2432..3382)
                     /locus_tag="NBI42_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1778035.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQIIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVIGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(3533..4420)
                     /locus_tag="NBI42_001617"
     CDS             complement(3533..4420)
                     /locus_tag="NBI42_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1778036.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSDDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKNGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDVQSIDETYNTFKQIGKLTHHEKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(4672..5307)
                     /locus_tag="NBI42_001618"
     CDS             complement(4672..5307)
                     /locus_tag="NBI42_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HCD1778037.1"
                     /translation="MLGTDELYKLLYRHMGPQNWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIRPSGFYKSKSLTIKSLLTWLTRYDFNYQ
                     EINERYKSKLRNELLSLKGIGSETADVLLVYIFGRIEFIPDSYARKIYDKLGYENTKS
                     YDQFKKVVTLPSHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(5624..7285)
                     /locus_tag="NBI42_001619"
     CDS             complement(5624..7285)
                     /locus_tag="NBI42_001619"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCD1778038.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGYVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLAAHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(7282..7698)
                     /locus_tag="NBI42_001620"
     CDS             complement(7282..7698)
                     /locus_tag="NBI42_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCD1778039.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(7791..7899)
                     /locus_tag="NBI42_001621"
                     /pseudo
     CDS             complement(7791..7899)
                     /locus_tag="NBI42_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7974..8984)
                     /gene="adhP"
                     /locus_tag="NBI42_001622"
     CDS             complement(7974..8984)
                     /gene="adhP"
                     /locus_tag="NBI42_001622"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCD1778040.1"
                     /translation="MRAAVVTKDHKVSIGDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            complement(9483..10004)
                     /locus_tag="NBI42_001623"
     CDS             complement(9483..10004)
                     /locus_tag="NBI42_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HCD1778041.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(10032..11327)
                     /locus_tag="NBI42_001624"
     CDS             complement(10032..11327)
                     /locus_tag="NBI42_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1778042.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVSPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(11367..11843)
                     /locus_tag="NBI42_001625"
     CDS             complement(11367..11843)
                     /locus_tag="NBI42_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1778043.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYQPEMYSEKLDLALMYKAF"
     gene            complement(12012..12548)
                     /locus_tag="NBI42_001626"
     CDS             complement(12012..12548)
                     /locus_tag="NBI42_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCD1778044.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTTETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(12758..13694)
                     /locus_tag="NBI42_001627"
                     /pseudo
     CDS             complement(12758..13694)
                     /locus_tag="NBI42_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
BASE COUNT         4513 a         2690 c         1836 g         5203 t
ORIGIN      
        1 gcggtgataa acctttatga ttaaagtaat cttctatatt ttctagtaaa attttatcgt
       61 taatacgcat tggtagtact cctttaaaat gaaacatctt tatttattgt attggtttaa
      121 tatccccttc gcaaatcata aaaattaata ttcactgtat tatgccatgt aataaaataa
      181 ccgacaagta ttgcgattta acttgtcggt taagcacgat taatttaatt attatttttc
      241 caaatcaata cgatataatt ttatgttttt atcttttggt gctgaggcaa attgataagt
      301 cgttttgcct ttttcaatat atccaaaatt accagcaact ttgctatatt ccgtatgtgt
      361 tactgctttg ccaacataat ttttaacagc ttgatatgtt ggaccattag catcattata
      421 catcattgaa atatgagtta ctttaccatt gtttttctta ccatcagtag ttactaataa
      481 catacctttt ttagtatgga attcgtaata atgttcagtt ccatcttcat tgtaagaata
      541 catcggattt ttatacttag ttaaaacatc tttaatagat tcgccaattt tcacattttc
      601 taatgactga tcacccttaa ttaattgttt aacagtatca atagagttgc cacttgctgc
      661 ttcggcacta gtgttcgcta tgcctactcc cattacaaca gaacatgcaa ttatacttgc
      721 cgttagtagc tttttcataa gcactctctc cttttattta tatcgtcttg tgctcacaac
      781 cattatacaa caatcatttc taaaacaaca gtcatattac aattatatta caaataataa
      841 ttactacttt tatatttcac ttatcactaa aaattaaaca tgcttttcat cgtgatgttt
      901 gttgataaat gaagctgtat cttcgacgaa tctttcttgc tcttcaacaa atgggaatgc
      961 acttgattct tgatatactt caaagtccgc attttggatt aaatcagcta cttctttagc
     1021 ttctaatctt gttgttcttt caccgaattc tcctgcaata atttttgtcg gtactacgac
     1081 atttctataa gtttgagaaa tatcagcgtt tttgaatacc tctttaactg cttggatttc
     1141 ttctttagtt gaaatttcat ttgtatctac gacatgtttg aggaatcggt tcatttttct
     1201 cggacgataa tacttacgct tatttaaaaa tttatcttgt ttttcaggat cccagtttcg
     1261 aataatatgg gcatattttc taaataaacg ttcttccggt aattcacctt caatagatgt
     1321 tggatttacc aacataagtg atgatgtaaa ttcaggataa cgtactgaaa tatccgcacc
     1381 aatgattcca cccatttcat ggcatacaaa tgcaacttct tcaatgtata aatatttaag
     1441 taattcaaca atgtcatcag aaaaatcttt tatttcaatg tgacgaggtt tatcagaata
     1501 gccatgtcca cgtaaatcaa ttaacacaac ttgaaatgat tttgccaatt ctgctgctaa
     1561 tttattaaac acagaataat tatcaagtac agtatgaatc aatacgatag gatagccttc
     1621 acctaatgta ctgtaatgta tcgatgttcc atcttttcta gtaaatagat ccataatttt
     1681 ctcctttatt atcatttttt attggtaaaa tggttaataa ttcttccact gttgtaattg
     1741 taaaatcaac ttcttcttcg agtggttcaa tttcagcatc ttcttgttta taccatacac
     1801 taaccatgcc catagctcgt gctggagcta catcatttaa cgcatcatcg ccaacataca
     1861 taatttgctc aggtaatacc cctagttgat caatcatatc ttcaaaaatt ttaggatgtg
     1921 gtttacgaaa accaactgtt tctgatgttg ataaataatt aataacatgc atcaaaccaa
     1981 gtgaatgtaa tcgaaattgt ttaatcttag atttaccatt tgcgataaca ccaatttgaa
     2041 agcctttttg cgatagcttt tctagtgtat ataaagtatc gtaataagga aatacataac
     2101 gataaaaatg catttcaaaa tcattaaata agtctttcca agttagccta tctacatgga
     2161 aacgtttaat gatttcttta tataaatcag gtttatcatt atcttcatcg tcatctagct
     2221 caataaatgc ttttttaaaa tctgctagtt gaacatgaat taagtagtca tgaaatcgtt
     2281 catattgctc ttcgataaat ttctctcgag atttaactct gtccaacaac gtaccttcta
     2341 aatcaaatac aactgcttta atttgattca aatccatcgc ttatgccctc tttatgttta
     2401 tagttatttt tattttagca aatgtcatgt gctagtgtaa ccgtttcgac tgagatatta
     2461 gcatgtaaaa taacactggt ataccaatta atgcaatgat aatacttgcc ggtatttgta
     2521 ctggtgacaa tatatggcta ccaataaaat ctgccataac cattatttgt cctccaatta
     2581 caatattcaa agccaattgc cttcctaatg attgtttcca ctgccatttt ctaatgagtt
     2641 gtggtatcac cataccaatg aatccaatga tgccaacata tgcgacattt atcgccgtta
     2701 tcatagaagc tatacataac gcgatatatg taattatttg aacatttaag cctagtgact
     2761 gactttttag ttctcctaat tgcaataact ttagttgatt aagaatgata aatataccaa
     2821 acagtgcaat gataaatgtg attgctatta tggatacatt tgagtattct gcatcaccaa
     2881 aaccaccaaa cagataattg gcaattgtat ttaatttacg aggttttaat aaaatcaaaa
     2941 aatatagaag tgaattgaat aatgcaccaa tcattaaacc acttaatatt aagattctaa
     3001 ctggatatcc ttggctcaat accgacgtaa tgactaatac agttattaat gttatcaaac
     3061 taaatgttat tgaaaataca ggaatccata acgttgttaa acctaaaaat aatgctaatc
     3121 ctgaaccaaa tgtagcaccg cttgccaatc ctaatgtaaa gctatctgcc aatgcattat
     3181 ttaaaactgt ttgaaatata agacctgcaa cagttaatat catacctgtc aacaatgctt
     3241 ctaataatct cggtaatcta acattgatta aaatagactg gttaaataca tcattgatat
     3301 cccccaactg ccaaaataga tatatgctag ttgttataat caatatgact atccagctca
     3361 ataatacttt attaaatgtc atagcattct cttttctttc atcgataatt acattcatta
     3421 tagcaatcac atactcgcgt tgctatcatt cactaaatat attttaaaaa ccccttatct
     3481 caaacatgta atttttacta catgtagcaa taaggggcat aattagaatg gtttattttc
     3541 tataaattgc atctcttaat tcttttaatc cttcatcaat acgtggacct ggtcttgata
     3601 cttcatcacc atttacaact tcaatacgtg tgttcttgac agcattaatt ttattgaatc
     3661 cacctctttt tttgatgata tccatataat ctgatcttgt cttagcttcc gtcgaaatta
     3721 atatatctgg gttcttttta ataatacttt ccttcgttac agggttccaa ccattaatgt
     3781 cactataaac attttgggct tctaattttt ctaacatatc attgaaaaat gtatgcttcc
     3841 ctgctgtata tatttcaggc tttgatgaaa cctcaataaa tacttttgat tttttatgat
     3901 gagcaggaat tgaatctatg actttatcga tattatcttt agtttcctca acaagttgtt
     3961 cagcctgctt ttcatgatgc gttaatttcc caatttgctt aaatgtgttg taagtttcat
     4021 caattgattg tacatcttta acatacacta ctttgatgcc ttgtttctct aatgatgaca
     4081 atactttatt agcagttgcc ttttgcgact catgcgcaag aattaaatct ggctttgctt
     4141 ttaaaagttc ttctttattt agattcaaag catcaaattg ttttttacca tttttcacat
     4201 cttttggata atcatcaaca gtagaaacac ctactatgta tttacccaac cctaattcat
     4261 ataaaatttc agtattacta ggcattaacg aaacaattct atggtacggc gatttagatg
     4321 agccattatc atcgctacct tgtttatcat tatctttcat accacagcca ctcagaacaa
     4381 gcataaaaat caaaataaaa gcaattaacg atttcttcac attatcacat ctccctttta
     4441 gagcccctta ttaattaggg gcttttatgc agttggtgcg ttagcaagcc aactgtaatc
     4501 ctctttaacc tttgagcttc agcgattaga ggcttttatg cagttgctgc acaaacaaac
     4561 aacttttttt ctttatatgc ttctatttat tatataagtc cacatactaa aagaaaatag
     4621 taaattattt tttatatttc atataaactc atctatctaa cttcacagcg tttacttttt
     4681 aaagtaaggt tctaaaaaat cataattctt tatatcttta tctctaaagt aatgtttacc
     4741 aaatacatct aacagagcat gaaattcatt agcatcttga tttgtaaaat gacttggtaa
     4801 tgtgactact tttttaaatt gatcataact tttagtgttt tcatatccta acttatcata
     4861 tatttttctt gcatagctat ctggaataaa ttcaatacgt ccgaatatat aaacaagtaa
     4921 aacatctgct gtttcacttc caataccttt caaagataat aactcatttc ttaatttact
     4981 tttataccgc tcgttaattt cttgataatt aaaatcatat cgtgttaacc atgttaataa
     5041 tgatttaatc gtcagtgatt tacttttata aaagccactt ggacgtatca atgattgtaa
     5101 cgtttcaata ggtagttcta atatatgatt tggattaaaa tgcgtgtgtt ctttaatctg
     5161 attcaatgca atttctgcat ttcgccatct agtattttga actaaaattg cacctaacat
     5221 catttcaatg tcattatcag caggccacca attttgaggt cccatatgcc tatataacaa
     5281 tttatacaat tcatcagttc ctaacattta caccctcctt ttagagcccc ttgagcttta
     5341 gcgattaggg gctcttatgc agttgtgcgc aagcaaacca actgtattcc tttaggggct
     5401 cttatgcagt tgttgcacaa gcaaaccaac tgtattcttt aacccccctt aagctttagc
     5461 gattaggggc tcttatgcag ttgctgcgca agcaaaccaa ctgtattcct ttaaccttat
     5521 tcacatacta agcattcccc aatatgtcac atacctaata aaaaaacact ccaacataaa
     5581 gtatctttcg agtaactcta cgttggagtg tatataaatg tttttacatt gattcaggtg
     5641 cgcttacacc aaccattgcc aatgcatttt tcaatgtgat tctaaccgct tcaatcatag
     5701 cgacatgtgc ttttgttttt tcaatatcat ctgttaatac tttttcagca ttgtagaatt
     5761 tatggaaatg agcagccaag tcttgaatat agttagtaat tctatgtgct gatctatgtt
     5821 ctgcagcact ttcaattgta ggctcaaagt cagctacttt tttcaacaat tcaatcgctt
     5881 tttcatttgt aatcgttgta aaatcgttcg cagcagtcac ttcaatacct tgctcttttg
     5941 cttgttttaa aattgaacaa atacgtgcgt gagcatattg agcataataa actgggttat
     6001 cttgagattg ctctttcgct aattccatat caaaatcaaa gtgactatca ggactacgca
     6061 tagttaagaa ataacgtgca gcatcaacgc caacttcatc cataatttct cttaaagtaa
     6121 tggcattacc agtacgttta ctcatcttca cttctttacc attttccatt aaacgaacca
     6181 tttgcatgat ttgaatttct aaacgattac tatctacacc aaacgtttca agagatgctt
     6241 tcaaacgatt aatataacca tgatgatcag caccaaataa atcgattaaa atgtcattac
     6301 cacgtttaac tttatcgaag tggtaagcaa tatctggtaa gaaatacgta tatgtaccgt
     6361 catttttaat taatactctg tctttgtcgt ctttaaaatc agttgtacgt aaccatgtag
     6421 cgccatcagc ttcatacgta taacctaatt ctttcatttt tgctaaaact tcaagaattt
     6481 cgcctttttc atataaggat gtttcactaa accaattatc aaaatgcgta ttgaactctg
     6541 ctaaatcatt tttcagttta gccatttcgt attctacgcc taattttcta aattctttca
     6601 aacgtgcttc ttcagaataa tctttaattt cagggtgttt ctctgctaaa tcttttccta
     6661 tttcaataat atcttttcca ttatagccat cttctggcat tgaataacta ttgtcaccta
     6721 aagcttcaaa gaaacgtgtt tcaatcgaac gcgctaagtt agtaatttga ttaccagcat
     6781 cattaatata atattcacgt gttacattat agccagctgc agttaaaata ttagctaaag
     6841 catcaccaac tgctgcattt ctagcatgac caatatgtaa atctcctgta gggttagctg
     6901 aaacatactc aagcaataca ttttgacctt ttgattcatt tacatatcca aattgatcac
     6961 ctttttcaat tgcttcagga ataattgctg ttaaatactg attatctaag taaaaattaa
     7021 tgaatcctgg accagcaata tcaatttgtt ttacatgtgc tttttcagtg tctaagttat
     7081 caacaatcgc ttgagcaatt tcacgaggat tacgctttgc aatcttagtc agtaccatcg
     7141 caatattagt agcataatct ccattttttg tatctttagg aacttcaatt ttaatatcag
     7201 gaatctcatc tgctaatcct gctttgttaa tacttgctgc aatttcttct actaatgttt
     7261 gtttcacttg atcaataata ttcattcgcc tatctcctta tagttaattt catattgata
     7321 agaacccatt ttttcattat cttgatataa ttcataatga atctttagct tgccaccgtc
     7381 tccacttacg aaatgtaaaa tgcgtaatgt tttaacttct agtggaattc gtccagccga
     7441 tatatcgtaa aaagttgttg tcgtttgtcc ttcaacaaaa tgcaaattca tattaatgtc
     7501 gcctttacga atcaacttaa cgctatcatc atccactttt attgtaacat taactgttgc
     7561 atcctcaatt tgttctacat accgaataaa gttagattgc ctttgttgcc aagttccttc
     7621 agtagtaaat tcaaattttt ctttttcgtt gtgctgtttc aacacttgct ttgtttgaat
     7681 acttactttt ttatccaatt tactactcac cttaatcttc gcataataga aatattatag
     7741 cataaaataa ggtcatcttg ttatgccttc ttaaagctaa ataggttcaa ttaattaaca
     7801 attccatact taatcaacgc ttcatctatg taagccattt tataaaatac aaacaacacc
     7861 tcaaaaccaa aaaaacacga gattttcact atctttcatg aaaacctcgt gtttgattat
     7921 aatgatagtg ttcagagcac taccttcttt atctatatag tcagttgata tttttaaaat
     7981 ttaataacca ttctaccagt tatagtacca ttttccattt cttcaaaaat atcattgatt
     8041 tcttctaatt ttcttaattg aactttaggt gttactttat tttcagcagc aaattcaaac
     8101 gcttcacgta agtcttgtct tgtaccaaca agtgaaccta ctacttcaat accatcaagc
     8161 actaatcttg ggatatctaa gttcatttta tcaacaggta aaccaacagc aacaactctt
     8221 gcaccagctt ttacaacatc aaccgcttgg ttaaatggcg tcttagcaac tgaagttacc
     8281 actgttgcat ctaatccttt gttatcagtt aatttcataa cttctgcaac tggatcaaca
     8341 tctttagaat taataatagc atctgcacct aattctttcg cgaatgctaa tttatcatca
     8401 ttaatgtcga aggcaacaat tttagccccc ataacgtttt tagcatattg taaagctagg
     8461 ttacctaaac cacctatacc aaaaacacct aaccattgtc caggttttac attacttact
     8521 tttacagctt tataagttgt cacacctgcg catgtaatag aagacgctgc tgctggatct
     8581 aatttttcag gtactttcac agcatagtct gcagtaacaa taacttgttc agccattgca
     8641 ccatctactg tataaccagc atttttcaca ctacggcaaa gtgtttcacg acctgttgta
     8701 caatattcac atcttccaca gctttcgaac atccaagcga tagacacacg gtctccaatt
     8761 tttaatgatt ctacatcttc tgcaacttcg atgactttac caataccttc atgacctaaa
     8821 gtaacgcctg taacatcacc aaaatcagca ttcttaacat gtaaatcggt atgacaaacg
     8881 ccacaatatt ccgtttgtac caatgcttca ccaggtttta aagctcttaa ctttttgtcc
     8941 ccaatactta ctttgtgatc tttcgttaca actgctgctc tcataataat gtcctcctta
     9001 tagtcactcc agtatgtatg atattaattg gcgattactt tcaaaaatga aaacgaataa
     9061 ttatagatgt aatttttatt tgtaatagac gccagattaa aatatactac tgtgactttg
     9121 tttttagtta gatatcaacc gttaactttt aacgtttccc tagttaatat gttaaaagaa
     9181 ataacataat cgatatgcta acgtttaatt tttacttcac atatatttat ttaaagataa
     9241 tttataatac tcttatcttc actcttatta taatgtgaat taattcacaa agtatatagc
     9301 tatgatcaaa aaaatgtgtc agttattcaa attttttatt aactacagat taatttaatt
     9361 tcaaaaaaat taaaaaaact cccaatcaat ctaagacatt attttcacct cacgatttac
     9421 gtcgtcattg agaaatgaat catatcttta tagattaatt gggaaatttt agtttctaat
     9481 atttaattaa atgcttgatg taaagcatca gaagaatgct tccacatacc ttcatcatgg
     9541 ttcattaaaa agtcttttaa aatagcttta ttctcgtcac caatttgatc aatgataata
     9601 tgatgtttca ttgatttatc catcatatta acatgttcag gcatactttt ataaccacga
     9661 cgaattgctt tattcactaa taaatagcac gctgttaaac cagcataata tggtccttgt
     9721 tcattacgtt ctgttgttac ccaacacaac caatattctt tagcttcatc gccttcaaca
     9781 gcagctttat cagtaatcca tttcacgcct ttttcaactt tggcgcgagc atgcatgcct
     9841 ccaatatcaa taaatgcttc tttgttagca acatcgataa aaactggtgc aatattgtct
     9901 aagctaattg aaccaatatt tgtgccttta tgaccatcta gagggtcatt ttttatgata
     9961 ttaaaattaa agcctttttt ctcagacaat gatataacct ccatatttaa cgataacaat
    10021 ttcagccctg tttaattaag ctcgttaatc aaattttcaa tagcttctct aattgtctta
    10081 tctttaatct ttgcaaccat ttctctagga taatataatt tatagtcttg gcgattaatg
    10141 cctgtaatac tatgaatgac taaagattga ttgcttattt ctctaatcgt accatcttgt
    10201 cttaataaat gaatcggttt gcgatttgac cccggtcgat catagtcata tggcaaatcc
    10261 gaaaatgctt cactcacaaa ataataatct ggattaatac cacctgcttc aaacagttct
    10321 tgcagttctg atattgtaat aattgagcca tcaaatggaa tatatttaaa taagtctcga
    10381 ttaataaatc gacttgctaa atcacttaaa atggcatcat cttcttttgt ccatttttcc
    10441 aaataatacg taactaccgc ttcatcgagt tcaacatatt gttcaatcgt aactgtttct
    10501 tcaaaaaatg gaataaaatc atgtggatgc aacttaaatt catagccttc attataaagc
    10561 tgttttgcgc gtttcaaaca attattaagc agcacttctc caccacgact aactgggtgg
    10621 aaataaatct gccaatacat ttgataacga ctcataataa agttttcaac tgcatgcata
    10681 ccactttctt tgattagtac ttcatcttta gaaggtcgca ttaatcttaa aatacgctcc
    10741 atatcaaaag caccatatga tacacctgtg aaatacgcat cacgttgtaa ataatccatt
    10801 ctatccgcat cgatttgtga cgaaatcatc gaaatgacca atttattatg atgcgtttta
    10861 ttaattactt ccgcaacttc tcttggaaac tcaggcgaca ctttacgtaa tacagcattc
    10921 acctcagtat ctccagtaat aatcgcttgt gtgtatgctt catggtctgt attaaatatt
    10981 ttttcaaaac tatgtgaaaa tggaccatgt cctaaatcat gtaataatgc agcacataat
    11041 gccaacggtc tatctttatt gtcccatgca tcatgaccaa taaatgactc atcaattaat
    11101 cgtctaacta tttcatacac acctaaagaa tgtccaaagc gactatgttc tgctgtatga
    11161 aaagataggt acagtgttcc tagttgtcta attcgacgta acctttggaa ttccttagtt
    11221 ttaattaaat cccatatcaa ttgatcttca acatgaatat atcgatgtat cgggtcttta
    11281 aaaacttttt cttctactaa ttttaaatct acatatgcgt tagtcattat tcccctcctt
    11341 ttcgtttaat ataatattta atttacttaa aatgctttgt acataagtgc taagtctaac
    11401 ttttcgctat acatttctgg ctgataagag cgtaagattg taaaaccttg ctctttatag
    11461 tatgctactg cttcttcatt tttattatct acttctaagt aaacaccttc aaatttatct
    11521 tcaaaacgtg ataatccttc atttaacaat gctgtaccat aacctgtatg ttgcgattct
    11581 ggtttaacat aatgagctga taaatataat tcttcaccgt aaataaagtt agcaaagcca
    11641 acgatgtcat taccttcttc aacgactaag aataattgtt cttgaagtct tttctttaaa
    11701 tgatgttcat tatatgaagc ttctaacaag tgattaactg ttgtcgcagc gtatatattt
    11761 aagtatgtat taaaccaagc tttagttgcg acatctctaa tttgaacaac atctttttca
    11821 gttgcttgtc ttaccttgaa catgactttc tccccttatt aacaagtttt aataacggca
    11881 ttataccaca acttgctcaa tacttaataa acaatgattg tctattcaat ttatatattt
    11941 atattttccg ttaaaattaa aaataaaaaa taacgaagca aaaaatcact tcgtttagta
    12001 tgaggtatgt cttattgcaa tatactattc cactcagttg cacgtgctaa ggcataattg
    12061 tctttcatga tgtcaccagg cttttcagca gttccaataa tataaccatt taaagtggca
    12121 cctaaaaagt ctaaactata tttcatttgc gtaattgctg gcttcgcttt tatttttggg
    12181 caatctccac caactaaaat aactctaaaa tccttttcgg ccatttgtgc cttaaaatta
    12241 ggataacgtt tatcttgtag tgtttctgac caatgttcta taaatgcttt caatggtgct
    12301 gaaatgctat accaatacac tggtgatgca aaaataattg tatcactagc caatatttta
    12361 tctagaatcg gcaaatagtc atcgtcatat gaagtaatag tctctgttgt atgtctcacg
    12421 tcacgtatcg gtttaaactg atgttgtgtc acgtcaatcc attgatactc taaatcttgc
    12481 aaagcgaatt ttgttaattg tgcagtatta ccgtttggtc tactcccacc aaacaaaaca
    12541 gtaatcattt tagcctaacc tcacttttga ttaataaata tctgtgtttt tcgttaccta
    12601 attatactat catatgcttt gcctaccgaa tagtaaaacg cttacaactt ttatataaat
    12661 ttgacgaaat ttcgtcatgc cttatataac gtcgtttgtg atacggggct aattcatgat
    12721 gaattagata catatgtcac cattaaatac aattcattta gtcttcaatt ggaaacagtt
    12781 catcgatata ttgaatctca tcatctgata aaacgatatc tgcagcttta atattttcaa
    12841 tgacttgttc tgcacgtttt gcaccaggaa taatcacatc gatagctggt ctcgttaaat
    12901 aaaatgctaa tacaatgttc gcaattgaag tttgatgtgc tgcagctatg ctttccaaag
    12961 cttttacgcg acgcacattt tcttcaaata cacctggttt aaaatcacga cgtgtagtac
    13021 gatggtcact aaatttagtg ttctcatcat attttccagc taaaataccg gatgctaatg
    13081 ggaaatatgg aataaatgtg atttggtgat caacacaata ttgtaatact tcctcatttt
    13141 cgcgatgcaa taaattattc taactgtaca acatcaacgt aaccatcttt atttgcctct
    13201 ttaagttgat ctaatgtgaa atttgataca ccaatgactt taatcttccc ttgttcctta
    13261 agctcttgta atgctgcaac tgcttgatct ttcggagtgt tgtcatccgg aaaatgaata
    13321 taatataaat cgatataatc agtttgtaga cgtttcaaac tattctcaac ttgttgtttt
    13381 aaatattccg gttgattgtt ctgatgtact tcttgatttt catcaaattc atgagaccct
    13441 ttcgtagcaa ttttaatttg ctctcgcgga tattctttaa caacttctcc aaccaattct
    13501 tctgatcgtt ctggcccata aatatatgcc gtatctaata aattaatacc atgattaatg
    13561 gcttgacgaa caacatcttt tccttgttct tcatctaagt tcggatataa attatgccca
    13621 cctactgcgt tcgtcccaag tgcaattgga aacacttcaa catcagattt acctaagttt
    13681 acaaattgct tcattagacc cagctcctta tctttataac ttttccacta tcacactacc
    13741 atgatttaat acaacttcca aatgttagaa ttagggtttg aattttgaag gtgtattagg
    13801 aaatcatcat atagaactca gttaaaataa gtattgctta agttgatatg catatagtgc
    13861 gattgctagt cagccgttaa attacatgta cttttgaatc agaaattgaa aaacatttaa
    13921 gaacgacaac attaaagcca ttgcaaattt aattagaaat actataatgc tttgatttgc
    13981 ataacaaaga aattaaaact gttaatatta ccatgggata tctattataa aatattcatc
    14041 tttgtagttg atttctatac ttaggatttt aattcattct atctatgagt caaattttta
    14101 tattaaattt aataatttta gacttataaa actcatttat aattcagctt tttttaagaa
    14161 gtattacatg agctttttta atgccaatgg gaacaatgtt tataaaaaag aaaaacatag
    14221 ttactgcacc ccaacagata aa
//
LOCUS       DAHHRR010000064        14212 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.064, whole genome shotgun sequence.
ACCESSION   DAHHRR010000064 DAHHRR010000000
VERSION     DAHHRR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.064"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            75..2780
                     /gene="acnA"
                     /locus_tag="NBI42_001628"
     CDS             75..2780
                     /gene="acnA"
                     /locus_tag="NBI42_001628"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCD1778045.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNEGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNDIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDTAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            2959..3426
                     /locus_tag="NBI42_001629"
     CDS             2959..3426
                     /locus_tag="NBI42_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD1778046.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(3633..3929)
                     /locus_tag="NBI42_001630"
     CDS             complement(3633..3929)
                     /locus_tag="NBI42_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HCD1778047.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4306..4914)
                     /gene="plsY"
                     /locus_tag="NBI42_001631"
     CDS             complement(4306..4914)
                     /gene="plsY"
                     /locus_tag="NBI42_001631"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCD1778048.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLPVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5120..7111
                     /gene="parE"
                     /locus_tag="NBI42_001632"
     CDS             5120..7111
                     /gene="parE"
                     /locus_tag="NBI42_001632"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCD1778049.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTDKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7111..9513
                     /gene="parC"
                     /locus_tag="NBI42_001633"
     CDS             7111..9513
                     /gene="parC"
                     /locus_tag="NBI42_001633"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCD1778050.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDSSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITIITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            9763..11223
                     /locus_tag="NBI42_001634"
     CDS             9763..11223
                     /locus_tag="NBI42_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCD1778051.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
     gene            11723..12574
                     /locus_tag="NBI42_001635"
     CDS             11723..12574
                     /locus_tag="NBI42_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCD1778052.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMVLNDQ
                     TNTIEKIYKLDSEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
     gene            12549..12716
                     /locus_tag="NBI42_001636"
     CDS             12549..12716
                     /locus_tag="NBI42_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778053.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            12709..13917
                     /locus_tag="NBI42_001637"
     CDS             12709..13917
                     /locus_tag="NBI42_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1778054.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
BASE COUNT         4966 a         1993 c         2632 g         4621 t
ORIGIN      
        1 gtttatcaat aaatataata atacaattta attaaggcat ataaatataa aaatgtatca
       61 agggggatca ttaaatggct gcaaatttta aagagcaatc aaaaaaacat tttgacttga
      121 atggccaaag ttatacttac tatgatttaa aagctgtaga agagcaaggt attactaaag
      181 tttccaattt accttattca attcgtgttt tgttagaatc tttacttcgt caagaagatg
      241 attttgtaat tacagacgat catattaaag ctttaagtca gtttggaaaa gatggaaatg
      301 aaggcgaggt accatttaaa ccttctcgtg ttattttaca agatttcaca ggtgtaccag
      361 ccgtagttga tttagcttct ttacgtaaag caatggatga cgttggggga gatattacta
      421 aaattaatcc agaagtaccg gtggatttag ttattgacca ctcagttcaa gtggatagct
      481 atgcaaatcc agaagctctt gaacgtaata tgaaattaga atttgaacgt aactatgaac
      541 gttatcagtt tttaaattgg gcaacgaaag catttgataa ttacaatgca gttcctcctg
      601 caactggaat agttcaccaa gttaacttag aatatttagc aagtgttgta catgttcgtg
      661 atgtagatgg tgaaaaaact gcatttccag atacattagt tggtactgat tcacatacaa
      721 caatgataaa tggtattggc gtactaggat ggggtgttgg tggtattgaa gctgaagctg
      781 gaatgcttgg acaaccttct tatttcccaa ttccagaggt tattggtgta cgactagtaa
      841 attcattacc acaaggcgca acagcaactg atttagcgtt aagagtaact caagagctac
      901 gtaaaaaagg tgttgttggt aaatttgtag agttctttgg tccaggtgta caacatttac
      961 cactagcaga ccgagctaca atcgcaaaca tggcgccaga gtatggagca acttgcggat
     1021 tcttcccagt tgatgatgaa tctcttaaat atatgaagtt aactggtaga tcagacgaac
     1081 atatcgcgct agtaaaagaa tatttgaaac aaaaccatat gttctttgat gttgaaaaag
     1141 aagatcctaa ttatacagat gttatcgaat tggatttatc aacagttgaa gcatcgcttt
     1201 caggaccaaa acgtcctcaa gatttaattt tcttaagtga tatgaaatca tcatttgaaa
     1261 attctgtaac agctccagcg ggcaaccaag gacatggttt agataaaagt gaatttgata
     1321 agaaagctga aattaacttc aaagatggat caaaagctac aatgaaaaca ggtgatattg
     1381 caatagcagc aattacatca tgtacaaata catctaaccc ttatgtaatg ttaggtgcag
     1441 gtttagttgc taaaaaagca gttgaaaaag gcttgaaagt tcctgaatac gttaaaactt
     1501 ctctagcacc aggatcaaaa gttgttaccg gatatttaag ggatgctggc ttacaacctt
     1561 atttagatga tttaggcttc aacttggttg gttatggatg tacaacttgt atcggtaatt
     1621 caggtccttt attaccagaa attgaaaaag cgattgctga tgaggaccta ttagtgacat
     1681 ctgtattatc tggtaaccgt aactttgaag gtcgtatcca tcctcttgtt aaagccaatt
     1741 acctagcttc accacagtta gttgttgctt atgcattagc tggaacggtt gatattgatt
     1801 tacaaaatga acctattggt aaaggtaatg agggtgaaga tgtatatttg aaagatattt
     1861 ggccatcaat taaagaagtt tcagataccg ttgatagtgt tgtaacacct gaattattta
     1921 ttgaagaata taataacgta tacaataaca atgaattatg gaatgatatt gatgtaactg
     1981 atcaacctct atatgacttt gatcctaatt caacatacat tcaaaatcca tcattcttcc
     2041 aaggattatc taaagaaccg ggtacgattg ttccattaaa tggtttacgt gttatgggta
     2101 aattcggtga ttctgtgaca actgaccaca tctctccagc aggtgcaatt ggtaaagata
     2161 cgccagctgg taaatattta caagatcacc aagtgcctat tcgtgaattt aattcatatg
     2221 gttcaagacg tggtaatcac gaagtaatgg ttcgaggtac gtttgctaat atacgtatta
     2281 aaaaccaatt agcgccaggt actgaaggtg gttttacaac ttattggcca acaaatgaag
     2341 taatgcctat ctttgatact gcaatgaaat ataaagaaga tggtacaggt ttagttgtat
     2401 tagctggtaa cgattatggt atgggttcat ctcgtgactg ggcagcaaaa ggtacaaact
     2461 tattaggtgt taaaacagtt attgcacaaa gttatgaacg tatccatcgt tcaaatttag
     2521 ttatgatggg tgtattacca ttagagttta aaaaaggtga atcagctgat tctcttggtc
     2581 tagatggtac agaagaaatt tctgttaata ttgatgaaaa tgttcaacca catgactacg
     2641 tcaaagttac tgctaagaag caagatggtg atttagtaga atttgacgct atggttcgtt
     2701 ttgactcact tgttgaaatg gattactatc gtcacggtgg aattttacaa atggttttaa
     2761 gaaataaatt agcgcaataa aaatagatat cacagtaaat ttttaatcgg tatttgaggc
     2821 actttgatag tgctcaaaaa tacctctata aactttatga tttaataggt cggttcaaaa
     2881 cctttgtgtt cttggccggc ctcttcttgt attttatgtt agaatgagat ataaagttaa
     2941 actgaaagaa ggcttaaaat gatatatagt attacagaaa tagaagcgcg ttatgctgaa
     3001 actgataaga tgggtgtaat ttatcacggg aattatgcaa cttggtttga agttgcgcgg
     3061 ttggattata tatcgaagtt aggttttagt tatgctgata tggaaaaaca aggaatcatt
     3121 tcacctgtga ctgacctcaa tgtcaattat aaaaagtcta ttttttatcc agaaaaagtt
     3181 aaagttaaaa cttgggttga aaaatattcg agattacgtt cagtgtataa atatgagatt
     3241 tttaatgaaa aaggtgaact tgcaactaca ggttccacag aattgatttg cattaaagaa
     3301 gatactttta agcctatacg gttggatcgt tatttcccag attggcatga agcctatagt
     3361 aaagtacaaa cgctcaataa tgaagggaaa acagtagaga taatggatgg tattgattct
     3421 ttataagtta ctataaatgt ttggagaggc aatattaaca attaatctat aaaaaatagc
     3481 aaatacgtta acattaatgt aattagcgtg aagataagct aatatatagc aaaaacgaac
     3541 ttgttcatac aacatcagat gtgttaaacc aaagtacaac ttgtgaggtt cagtacacat
     3601 cagaatgtgt aaacaagttc gttttatttt agttattttg tggaataaga aatttcatct
     3661 tcgtgatcaa ctacatttac aataatgtgg tcatcttcaa agtaccacaa atctttttcc
     3721 gctacgacaa catttaaatc gtcatattgt tgttcatagc caatatcaac atcttccttt
     3781 ggttcaactg taaaagcagg actaaatcct tgcttgagtt ggaattcgcc accatatctt
     3841 acaaaaaaca ccagcacttt attattttca ggtaactcaa gttcattttt aaaccaagtt
     3901 actgctgcat cagtaagttc tatttgcata acaatgacct cctatattta ttacagaatg
     3961 ggagtgggcc ccaattcata ttgtttgtgg aatttctttt cgaaattctc tgtgttggag
     4021 cccaccccaa cctgcacatt attgtaagct gaatttccat aagcttctat gttgggaccc
     4081 tcgaccagaa ttgaaaaaac ctagttataa gcgcatttcg ttctgtcaac tacagctaat
     4141 ataacattgt ggagcctatg acattgattt atgtctcggt ctcaaatgtt cttgtaaaaa
     4201 actaagtata gttgaatttt aagttaaaaa aattgttttt gcaaaaataa aataatccat
     4261 caactgcaaa aaaacatgca atgatggact aatgtcaaaa aattgttaca tccattttat
     4321 tttaggttct tcgcctctaa aaatccttgc gatattagag cgatgtctaa ttatcaatat
     4381 gattgaaact aagaaactaa cgactaataa aatatagtct tgaatgataa gcgagccaat
     4441 cacacagcaa attgctgcaa cgatacttgc taaagaaaca tatttaaaaa tcttcaatac
     4501 aataaagaag ataattgcaa gtattagtaa aagtatcggg ttgactccca agacgacacc
     4561 tgcactagtt gcaaccgctt tgccaccttg gaattttaaa taaacaggat aaacgtgtcc
     4621 aagtatagcg aataagccaa caattaaacc atttgtaaaa aaagtactaa tagggccatc
     4681 tgcgtgaact ggtaaccata aagggaagaa aacagttatg aaccctttga aaatatctag
     4741 aaatgttacc aagaatcctg caggacgacc taatactcta aagctattag tagcgccagt
     4801 attaccacta ccaaattgtc taatatcttt tttgaaaaat aattttccaa ttacgaatcc
     4861 acttgggaaa gcgccgataa gataacttag tagtaacatg acgattatca tcataaatat
     4921 tacacatcct ttaatatctt aggactattt tatcatattc gtttaaatta cggctaaaat
     4981 ttttaaaaac ggggattaat atatggaatt aagctatgaa agttaattga tacttgcatt
     5041 ttacgctgat ttatataaga ataactattg tatagtttta aaaacgaacg tacgtttgca
     5101 ggaggcgaaa tcattggcaa tgaataaaca aaataattat tcagatgatt caatacaggt
     5161 tttagagggg ttagaagcag ttcgtaaaag acctggtatg tatattggat caactgataa
     5221 acggggatta catcatctag tatatgaaat tgtcgataac tccgtcgatg aagtattgaa
     5281 tggttacggt aacgaaatag atgtaacaat taataaagat ggtagtattt ctatagaaga
     5341 taatggacgt ggtatgccaa caggtataca taaatcaggt aaaccgacag tcgaagttat
     5401 ctttactgtt ttacatgcag gaggtaaatt tggacaaggc ggctataaaa cttcaggtgg
     5461 tcttcacggc gttggtgctt cagttgtaaa tgcattgagt gaatggcttg aagttgaaat
     5521 ccatcgagat ggtaatatat atcatcaaag ttttaaaaac ggtggttcgc catcttctgg
     5581 tttagtaaaa aaaggtaaaa ctaagaaaac aggtaccaaa gtaacattta aacctgatga
     5641 cacaattttt aaagcatcta catcatttaa ttttgatgtt ttaagtgaac gactacaaga
     5701 gtctgcgttc ttattgaaaa atttaaaaat aacgcttaat gatttacgca gtggtaaaga
     5761 gcgtcaagag cattaccatt atgaagaagg aatcaaagaa tttgttagtt atgtcaatga
     5821 aggaaaagaa gttttgcatg acgtggctac attttcaggt gaagcaaatg gtatagaggt
     5881 agacgtagct ttccaatata atgatcaata ttcagaaagt attttaagtt ttgtaaataa
     5941 tgtacgtact aaagatggtg gtacacatga agttggtttt aaaacagcaa tgacacgtgt
     6001 atttaatgat tatgcacgtc gtattaatga acttaaaaca aaagataaaa acttagacgg
     6061 taatgatatt cgtgaaggtt taacagctgt tgtgtctgta cgtattccag aagaattact
     6121 acaatttgaa ggacaaacga aatctaaatt gggtacttct gaagcaagaa gtgctgttga
     6181 ttcagttgtt gcagacaaat tgccattcta tttagaagaa aaaggacaat tgtctaaatc
     6241 acttgtaaaa aaagcgatta aagcacaaca agcaagggaa gctgcacgta aagctcgtga
     6301 agatgctcgt tcaggtaaga aaaacaagcg taaagacact ttgctatctg gtaaattaac
     6361 acctgcacaa agtaaaaata ctgataaaaa tgaattgtat ttagtcgaag gtgattctgc
     6421 gggaggttca gcaaaacttg gacgagaccg caaattccaa gcgatattac cattacgtgg
     6481 taaggtaatt aatacagaga aagcacgtct ggaagatatt tttaaaaatg aagaaattaa
     6541 tacaattatc cacacaatcg gggcaggcgt tggtactgac tttaaaattg aagatagtaa
     6601 ctataatcgt gtaattatta tgactgatgc tgatactgat ggtgcgcata ttcaagtgct
     6661 attgttaaca ttcttcttca aatatatgaa accgcttgtt caagcaggtc gtgtatttat
     6721 tgctttacct ccactttata aattggaaaa aggtaaaggc aaaacaaagc gagttgaata
     6781 cgcttggaca gacgaagagc ttaataaatt acaaaaagaa cttggtaaag gcttcacgtt
     6841 acaacgttac aaaggtttgg gtgaaatgaa ccctgaacaa ttatgggaaa cgacgatgaa
     6901 cccagaaaca cgaactttaa ttcgtgtaca agttgaagat gaagtgcgtt catctaaacg
     6961 tgtaacaaca ttaatgggtg acaaagtaca acctagacgt gaatggattg aaaagcacgt
     7021 tgagtttggt atgcaagagg accaaagtat tttagataat tctgaagtac aagtgcttga
     7081 aaatgatcaa tttgatgagg aggaaatcta gtgagtgaaa taattcaaga tttatcactt
     7141 gaagatgttt taggtgatcg ctttggaaga tatagtaaat atattattca agagcgcgca
     7201 ttgccagatg ttcgtgatgg tttaaaacca gtacaacgtc gtattttata cgcaatgtat
     7261 tcaagtggta atacacacga taaaaatttc cgtaaaagtg cgaaaacagt cggtgatgtt
     7321 attggtcaat atcatccaca tggagactcc tcagtgtacg aagcaatggt ccgtttaagt
     7381 caagactgga agttacgaca tgtcttaata gaaatgcatg gtaataatgg tagtatcgat
     7441 aatgatccgc cagctgcaat gcgttacact gaagctaagt taagcttact agctgaagag
     7501 ttattacgtg atattaataa agagacagtt tctttcattc caaactatga tgatacgaca
     7561 cttgaaccaa tggtattgcc atcaagattt cctaacttac tagtgaatgg ttctacaggt
     7621 atatctgcag gttacgcgac agatatacca ccacataatt tagctgaagt gattcaagca
     7681 acacttaaat atattgataa tccggatatt acagtcaatc aattaatgaa atatattaaa
     7741 ggtcctgatt ttccaactgg tggtattatt caaggtattg atggtattaa aaaagcttat
     7801 gaatcaggta aaggtagaat tatagttcgt tctaaagttg aagaagaaac tttacgcaat
     7861 ggacgtaaac agttaattat tactgaaatt ccatatgaag tgaacaaaag tagcttagtt
     7921 aaacgtatcg atgaattacg tgctgacaaa aaagtcgatg gtatcgttga agtacgtgat
     7981 gaaactgata gaactggttt acgaatagca attgaattga aaaaagatgt gaacagtgaa
     8041 tcaatcaaaa attatcttta taaaaactct gatttacaga tttcatataa tttcaacatg
     8101 gtcgctatta gtgatggtcg tccaaaattg atgggtattc gtcaaattat agatagttat
     8161 ttgaatcatc aaattgaggt tgttgcaaat agaacgaagt ttgaattaga taatgctgaa
     8221 aaacgtatgc atatcgtcga aggtttgatt aaagcgttgt caattttaga taaagtaatc
     8281 gaattgattc gtagctctaa aaacaagcgt gacgctaaag aaaaccttat cgaagtatac
     8341 gagttcacag aagaacaggc tgaagcaatt gtaatgttac agttatatcg tttaacaaac
     8401 actgacatag ttgcgcttga aggtgaacat aaagaacttg aagcattaat caaacaatta
     8461 cgccatattc ttgataacca tgatgcatta ttgaatgtca taaaagaaga attgaatgaa
     8521 attaaaaaga aattcaaatc tgaacgactg tctttaattg aagcagaaat tgaagaaatt
     8581 aaaattgaca aagaagttat ggtgcctagt gaagaagtta ttttaagtat gacacgtcat
     8641 ggatatatta aacgtacttc tattcgtagc tttaatgcta gcggtgttga agatattggt
     8701 ttaaaagatg gtgacagttt acttaaacat caagaagtaa atacgcaaga taccgtacta
     8761 gtatttacaa ataaaggtcg ttatctattt ataccggttc ataaattagc agatattcgt
     8821 tggaaagaat tggggcaaca tgtatcacaa atagttccta tcgaagaaga tgaagtggtt
     8881 attaatgtct ttaatgaaaa ggactttaat acagatgcat tttatgtttt tgcgactcaa
     8941 aatggcatga ttaagaaaag tacagtgcct ctatttaaaa caacgcgttt taataaacct
     9001 ttaattgcta ctaaagttaa agaaaatgat gatttgatta gtgttatgcg ctttgaaaaa
     9061 gatcaattaa ttaccatcat tactaataaa ggtatgtcat taacgtataa tacaagtgaa
     9121 ctatcagata ccggattaag ggcagctggt gttaaatcaa taaatcttaa agctgaagat
     9181 ttcgttgtta tgactgaagg tgtttctgaa aatgatacta tattgatggc cacacaacgc
     9241 ggctcgttaa aacgtattag ttttaaaatc ttacaagttg ctaaaagagc acaacgtgga
     9301 ataactttat taaaagaatt aaagaaaaat ccacatcgta tagtagctgc acatgtagtg
     9361 acaggtgaac atagtcaata tacattatat tcaaaatcaa acgaagaaca tggtttaatt
     9421 aatgatattc ataaatctga acaatataca aatggctcat tcattgtaga tacagatgat
     9481 tttggtgaag taatagacat gtatattagc taaaaactat atgcaatcac gaaattaaat
     9541 gataaaatac agtaatgtta aattttgact aaattcaagg gatttatatt aaatgctgac
     9601 caagtactta tcgttaaatt agcgatatac tttgaccaaa ctaatataaa tctcttgtat
     9661 tgtataagaa atgatgacgt atctctatat ttactgaagt ttcaattcgg tctaatttta
     9721 tagaagatgc atgattcatg tagaggatgc gaggggttta ttttgaaaga tttcgatagt
     9781 ttaattcctg gatggtttaa agaatttgtc catgttggta ccgatctaat atggtctcaa
     9841 tatttaattg gtctattatt gacagctgga ttcttcttta caattagttc taaattcgtc
     9901 caattacgaa tgttacctga aatgtttaga gctttagtag aacgaccaga aactttagaa
     9961 gatggtaaga agggtatttc gccattccaa gcatttgcga ttagtgctgg ttcgagagtt
    10021 ggtactggta atattgctgg tgttgcgact gcgattgttt taggaggtcc aggtgcagta
    10081 ttttggatgt gggttattgc atttataggt gcagcgagtg catttataga agcgactttg
    10141 gctcaggttt ataaagtgca tgataaagat ggtggattcc gtggtggtcc agcttactat
    10201 attactaaag gtttaaatca aaaatggcta ggtatcgtat ttgcgatttt aattacaatt
    10261 acatttgcat ttgtatttaa cacagtgcaa tctaatacaa ttgcggagtc gttaaatacg
    10321 caatataata ttagtccagt aatcacaggt attattttag caatcgtaac agctattatt
    10381 atatttggtg gtgtacgtag tattgctacg ttatcttcgt taattgtacc gattatggct
    10441 atcatttaca ttggtatggt tttagtaata ttgctattta atttagatca aattgttcct
    10501 atgataggta cgattattaa aagtgcattt ggtatcgaac aagtaactgg tggcgctgta
    10561 ggtgctgcgg ttcttcaagg tatcaaacgt ggtttattct ctaacgaagc tggtatgggt
    10621 tctgcgccaa atgcagctgc tactgctgca gtgccacacc cggtcaagca aggtttaatc
    10681 caatcattag gtgtgttctt tgatacaatg ttggtttgta cagcaactgc aatcatgatt
    10741 ttactatatt caggattaaa atttggtgat aacgcacctc aaggtgttgc agttacccaa
    10801 tcagcactta atgaacattt aggttctgct ggaggtattt tcttaacaat agcagttaca
    10861 ttattcgcct tttcatctgt ggtaggtaat tactattacg gtcaatctaa tattgaattt
    10921 ttatcaacaa accgtgtaat attatttatt tttagatgtc ttgttgtagt acttgtcttt
    10981 gtcggtgcag ttgtaaaaac agaaacagta tggaatacag cagacttatt tatgggctta
    11041 atggcaattg taaacattat ttccattata ggactgtcca atgtagcttt tgcattgatg
    11101 aaagattatc aaaagcagaa aaaagaaggc aagaatcctg tttttaaacc tgaaaactta
    11161 gaaattaact tatttggaat tagtgcttgg ggcgctaaca aatataagaa ctctgataaa
    11221 taaatatttt taatgtaacg attcatttta taaaaatcaa ataaaccctg aggttttcaa
    11281 attaaagcga aaatctcagg gttttaaatt tgtactcaat aatcatctgt atcattacac
    11341 tagttaatct aagatttgat taaatattaa tatagtaaga ttgagattta tttcctccat
    11401 tggtgtgcaa agactatacc attgataaga catattgttg gatagatgaa tataaattta
    11461 ttttagcttt gcacaattta atagtacgtt ttagtttgta ttgagtatta attcagataa
    11521 tatataaata aatagcatta tttgtaaata tgggagtcag atttttttat tgaagttatt
    11581 ccactttgag aggcatcgaa attaaattaa gatatatcat ataggatagg cgtgtaatag
    11641 aacacaagta tttaaattat tatactttta agttagacat ttccgatata taatcttaat
    11701 aatagaaaaa taggtgatga taatgggaga atatattgtt actaaaacat tgaacaacaa
    11761 tgtcgtagta tgtactaata atgatcaaga agttatttta atcggtaaag gtattggttt
    11821 taacaaaaaa gagggaatgg tgttaaacga ccaaactaat acaatagaga aaatttataa
    11881 attagatagt gagcaacaaa aagcacatta taaaagttta gttgaaatcg ctgatgataa
    11941 tgtattacaa gtaattattg attcgttaaa ttttatttct aatactgcga tgaatgttga
    12001 ttcaaaacaa cttgtagttt cattaacgga tcatattata tttgcatata aacgcttaaa
    12061 acaaaatcaa gttattagca atccatttgt tatggaaact atgcagttat atagtgatgc
    12121 atatcatatt gctaaacagg tgattgatca gttaaatgca gcattagatg tacattttcc
    12181 tgaagatgag ataggattta ttgcattaca tattgcatct aacacagaag atttatctat
    12241 gcatgagatg actttgatca ataatgttat taaaaaaggt atagatatca ttgaatcaga
    12301 ccttgtgaca actgttgata aggaatcatt acaataccaa cgttttataa gacatgtaca
    12361 atttttaatt cgccgattaa gaagaaaaga atatatacat gcacaagatg attttgtgtc
    12421 tatgattaaa aatcactatc cgatttgcta taacacagca tataaaattt taactatgat
    12481 acaaaaacaa tttgatgtta atatcagtga gtctgaaatt atatatttaa cattacacat
    12541 tcatcatttt gaagaaagga ttaatcaatc ctaaattaag tgtattacac gggcgtttcg
    12601 ttactgtaaa gggtatttgt atggtatcat tgatgttagt aattttacat aagtggactg
    12661 tttttataca acatcttatg aatattttaa gtggtggtga atctataatt gaatgaaaat
    12721 gaaaagaata taagaaagaa ctttttaaaa tttaccgaat cacggtatat gaagtttgtt
    12781 ggtgggaatg atttagtctt ctcattaata gcactagtat tgttgggtat tgttattttt
    12841 attttcgaaa aagtatcata cgtttttgat ccttttatca tcgtttttaa gacgatagca
    12901 gcacctatca tcgtctcttt aattctattc tatttattta acccaatcgt aaatatgatg
    12961 gagcgttata gaataccaag agttgcaggt atttctatta tttatctagc tgtagtaggt
    13021 gttattacgt taattgttaa tttattgata cctattattg gttcgcaagt agatagttta
    13081 gttaaaaatt caccgcaata tctagaaaaa ttaattaatt ctattgataa aatagcaaat
    13141 aatacgtttt tctcttcgta ttatagtcaa attaatgatt ggttaaattc tttacctaag
    13201 aaaataccat ctatgttaag tgaatttaca gatggctttg ggtctaaaat tgcaacgttt
    13261 gcagaaacga ttgctaatat tggcgttgtg attgtcacaa caccatttgt actattcttt
    13321 atgcttaaag atggacatca cttcaaagaa ttttcaacga atattatgcc accgaaattc
    13381 cgaaaagatt ttcatgatct acttgaaaaa atgagtgttc aagtgggttc atacattcaa
    13441 ggacaaatta ttgtttcatt ctgtatcggt atactgttgt ttatcggtta ttcggttatc
    13501 gggttgaaat atagcttagt attagctagt attgcggcag ttacaagtgt tgtgccatat
    13561 ttagggccta ctatagcgat ttcaccagct attgtaatag ctgctataac atcgccgtgg
    13621 atgctcttaa aattagcagt agtatggact ttagtacaat ttgttgaagg gcacttcatt
    13681 tcaccaaata tcatgggtaa aacacttaag attcatccac ttacaattat tttcatttta
    13741 ctgtgtgcag gcaaattgct tggtattgta ggcgttattt taggtattcc gggatatgct
    13801 attttaaaag tattagttac tcatttattc caattattta aacgtcgata caatcgtttc
    13861 tatggtaatg atgtaggtga atatgatatt aaagaaagta ataaaatagt tgaataagta
    13921 attaaaaata ccaatagact atacgtttgt tggtattttt atggtgctta taatgttggg
    13981 cactcacatt tatgattatc agcacatgtt tagcttataa atttttacga aagtttagcc
    14041 gtttataaag cacatacata atgaaacgag tatttgccac ttgattagta cttcattatt
    14101 atgtcgaaaa taaaaacaag tggtattttt aatatattaa gaagcactca taatcggctg
    14161 ttaattaata atatttttca taagtattga ttcatcattt tctttatgtt aa
//
LOCUS       DAHHRR010000065        14110 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.065, whole genome shotgun sequence.
ACCESSION   DAHHRR010000065 DAHHRR010000000
VERSION     DAHHRR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.065"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..627
                     /locus_tag="NBI42_001638"
     CDS             <1..627
                     /locus_tag="NBI42_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1778055.1"
                     /translation="QYITSLISTIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGGRKV
                     FFVDLLTDLNFTLKSYIQGQVTVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPF
                     LGSWLSFAPAAILGIIDSPTTFIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIV
                     VILAAGDLGGFTLVLIAVPLYAVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            complement(824..1594)
                     /gene="fabI"
                     /locus_tag="NBI42_001639"
     CDS             complement(824..1594)
                     /gene="fabI"
                     /locus_tag="NBI42_001639"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCD1778056.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(1872..3716)
                     /locus_tag="NBI42_001640"
     CDS             complement(1872..3716)
                     /locus_tag="NBI42_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HCD1778057.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYSAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            complement(3726..5111)
                     /gene="mgtE"
                     /locus_tag="NBI42_001641"
     CDS             complement(3726..5111)
                     /gene="mgtE"
                     /locus_tag="NBI42_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCD1778058.1"
                     /translation="MSMNTDEKERVQEELYDETLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            complement(5132..5986)
                     /locus_tag="NBI42_001642"
     CDS             complement(5132..5986)
                     /locus_tag="NBI42_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD1778059.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMPNDILEIHLPQEIPSVNLIPYARKVEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKGNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDSIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            complement(5983..6792)
                     /locus_tag="NBI42_001643"
     CDS             complement(5983..6792)
                     /locus_tag="NBI42_001643"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCD1778060.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHHYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(6809..7444)
                     /locus_tag="NBI42_001644"
     CDS             complement(6809..7444)
                     /locus_tag="NBI42_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCD1778061.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(7461..7808)
                     /locus_tag="NBI42_001645"
     CDS             complement(7461..7808)
                     /locus_tag="NBI42_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778062.1"
                     /translation="MRLYINEIKIKDDTLYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            7994..8587
                     /locus_tag="NBI42_001646"
     CDS             7994..8587
                     /locus_tag="NBI42_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCD1778063.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            8691..9056
                     /locus_tag="NBI42_001647"
     CDS             8691..9056
                     /locus_tag="NBI42_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HCD1778064.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHMAFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            9079..9885
                     /gene="yjbH"
                     /locus_tag="NBI42_001648"
     CDS             9079..9885
                     /gene="yjbH"
                     /locus_tag="NBI42_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HCD1778065.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLTIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(10345..12153)
                     /gene="pepF"
                     /locus_tag="NBI42_001649"
     CDS             complement(10345..12153)
                     /gene="pepF"
                     /locus_tag="NBI42_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCD1778066.1"
                     /translation="MSQQLTREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVESELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLESDDRKLRES
                     AYRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHTIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(12201..13187)
                     /locus_tag="NBI42_001650"
     CDS             complement(12201..13187)
                     /locus_tag="NBI42_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCD1778067.1"
                     /translation="MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHF
                     AHKHLAEQKCFNNETIKHYKSKLILAQMIQQQGCKVEIEPFLKEIKQIPDILINNKYV
                     IELQYSPIPYKQMLQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSMFINPITRKL
                     HTFNLEKKQIMMFQQIQYIGGHKYVAEKRNAKISELFNEAPCDYHAVYKLSKFAINQY
                     IKYCRWQNSVLEPTLSAMYQLQLTDQEVVHNYGYIFPEQIYIENHPIEWQLQVDLWLK
                     NGKSKLVSDNLNYFKLKKFIVALESKTAIIEKLINNYLNISSDRGNDVQILF"
     gene            complement(13308..14027)
                     /gene="mecA"
                     /locus_tag="NBI42_001651"
     CDS             complement(13308..14027)
                     /gene="mecA"
                     /locus_tag="NBI42_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCD1778068.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPLTTEFEDLLYMVDGTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
BASE COUNT         4683 a         2509 c         1937 g         4981 t
ORIGIN      
        1 cagtacatta catcattaat ttcaaccatc ttcttattga ttatggttcc attcttctta
       61 atttatatgt taaaagacca tgaaaaattt atcccagctg ttgctaaatt tttcaaaggt
      121 ggacgtaaag tatttttcgt agatttatta actgatttaa actttacatt aaaatcttac
      181 attcaaggtc aagtaactgt cagtgttatt ttaggtatat tcttatatat cggttattca
      241 attattgact taccatatat tcctttatta gtactatttg ctggtgtcgc aaacttaatt
      301 cctttcttag gttcatggtt atcatttgca cctgcagcaa tactcggtat tatagatagt
      361 cctactacat ttatatgggt ttgtatcatt acattaattg cacaccaact tgaaggtaat
      421 atcattacac caaatgtaat gggtaaatct ttaagtattc atcctttaac aattatcgtt
      481 gttattttag cagcaggaga tttaggtggc tttacattag ttctgattgc agtgccatta
      541 tatgctgtac ttaaaacggt tgttagtaat attttcaaat accgccaacg cattattgat
      601 aaagcaaaca gtaatgttaa ggactaattc tgtggatgtc ttttaataat ataagatact
      661 atcgcctcaa aagctgtagt ttcaactttt gaggcgattt ttttgtgcat aaaaaaatca
      721 gtcaaatgaa atatcaaata attttccatc agtccgatta ttataaagac aaaaaaagct
      781 ttggtcacat atataataac gtgaacaaag ctgttgaatg atattattta attgcgtgga
      841 atccgctatc tacatgaata ttttcacctg taacgccact tgataagtca cttaataagt
      901 aagccgctgt tttacctact tctacttgat caacgttacg ttttaaaggt gcacgctctt
      961 cgatttcttt aagaattgta ttgaaaccac ccacaccttt tgcacttaat gtacggattg
     1021 gaccagctga aattgcatta acgcgaatat tatctggacc taagtctaat gctaaatatt
     1081 taacatttgc ttctaagctc gctttagcaa cacccatcac attgtagttt tgaactgcga
     1141 attcgccacc taaatatgtt gttgcaacaa tgctaccacc ttctggcatt aattttttag
     1201 cttcatgagc aacgatagtt agtgagtaag aactaatgtc ttgggctaac aagaaacctt
     1261 cacgtgaagt ttcagaaaaa cgtccgcgta agtcttccat attagcaaat gcgattgaat
     1321 gatatacacc atcgatattg ccaacatctt taccaatttg ctcaaaacca ttaataacct
     1381 cttcatcgct ttgaacatca atttgatata agtgcgcttc tggttgattt aattgttcta
     1441 ataatttttc aagctcttta cggctacgtt ctttacggta agtaaatact aatttagcac
     1501 ctaattgatc taaaacttta gcgacaccaa aagcaatact acgcttatta gcgattccca
     1561 tgatgacata tgttttgttt tcaagattta acatgtaaga taactcctta taataattaa
     1621 tttaacggat agaatatcta atcccagttt taactaactt tttattcgtc aatacatttt
     1681 aacactatta tgacttttta ataaagttat ttaaacgtat tacaacgata aatgtttgat
     1741 ttagtccttt ctgtatcaaa cttgatttac gtaaaaagca tgtatttatc aaaatcatat
     1801 acatacaagc attttaaaat cattaatttt acgtcgcact aaaatacgtc tgcattttaa
     1861 tatcattatt gttaaaaata aaattctaac tcttgcttca attcatcaac gtattcttta
     1921 gcaccggtaa caattaagcg atctccatat cgcaattgtg tatctccatg cggaacaatc
     1981 gactcattat tacggataat acgcacgaag atgatgtctc ctccgaatgg gaaattacgt
     2041 aattgaatat tttcatattt ataatttaac atttgaattt catatagtga tgtttcaaca
     2101 ttacttaata aattcaacat gttaggtgtt tcaattaaac cttttaataa gattttatta
     2161 cttaagtagc tactgaaaat ttcaatacct gaatcaacta actctgtatc gtccgttgtg
     2221 ctttcaagtc tgcaaatgac acgctcaact tgatgtgctt tggctaattt agcaactttc
     2281 cggttaatat catcgtcatt cgtggcacaa acaactatgt ctcggtcaaa cagacctagt
     2341 cgttctaaaa catcttgttc ataatcagca atttctatca tagtgatatc atccgacaat
     2401 tgacgacgat cactcaagtc tttgcgataa tataatgtca cgtcatataa ctgagatgtt
     2461 aaattttgcg caataggaat cgttaattga tttttaccaa ttaaactaac ttcaatttta
     2521 cgattaaatt catctggaac tggaaacagt tttttgaaaa taatcggaac gaatacacat
     2581 gtaatgactg cgcttaaaat taatatacct gacgtttcag ctgaaatagc atttaatctt
     2641 tcagagatct tagcagcagc aatcacgagc gataatgttg atgttaataa aaatgctgat
     2701 gcaatcgttg ttttcatatc aaaccaacgt cgaataaaca tgactggaat taattttgaa
     2761 atgataaatg caacgattaa aatcggtatg ataattagta atttcggttc tttaattaat
     2821 gatggtatgt ttaaatctac accaaccatt ataaagaaaa taggaataaa gaacccataa
     2881 ccaaatgagt ctaacttttc aaccatttct tcatctggat ttaataatga aacaacgaca
     2941 cctgctaaga atgcacctaa tatattttct gcgccaactc cctctgctag ggctactaat
     3001 aatattatta atgcaaacac cgcacgaata ccgatttgcg tcgtaccatc catcaatttt
     3061 tgtaaaaatg acattctttt aaattgaaca cctaaaatat atgaaattgc tgtgaaaaca
     3121 actaatatac ctattaacca tattgtactg ccgccttgac cattgattgc actatagacc
     3181 gttaataaaa tcatagttac taagtccgca agtactgcta ctaataggat aaattgacct
     3241 atagtagttc tcataatatt catttctttt aaagttggaa caactacgcc taaggaaata
     3301 gttgaaatga taatgaccat taataacaca tcatctacta atccaagcca tttaaataca
     3361 tacgctaaaa gaatcgaaat aatcataata aatgcaaata cagttaacgc tagattaaga
     3421 tgccctggaa ttgaggattc atcatcattt tgtccttgac gtgcgcgttt atctttttta
     3481 aaagctttaa aatcaatttc taaaccactt aaaaacatta aaaagataaa gcctaacgtt
     3541 gataaaatat ttagaattga atccctttct actatattta gaaatgaatt tccaatcaca
     3601 atccccatca aaatttctgc aacaacaact ggcaagaaat taatatttaa tcgattgaca
     3661 attattggag ttaaaaacgc tgctaaaaca acaataacta aagataaaaa ctccataact
     3721 cctccttaaa ttaagtaagc cataaatgat gtagctaaac caaaataaat caacatacta
     3781 ataatatcat taattgttgt aataaatggc ccgctagcca ctgctggatc gatattcaat
     3841 ttattcatca ataatggaat catcgaacct acaaacgtcc ccaccgtcat cgcacaagtt
     3901 aaacttcctg caacgattaa tgctaaaagt ggctgatgat atattgcaac aataattgta
     3961 aataatattg ttgaacatac aacacccgat aacacaccac ttcccgcttc tcttagtgca
     4021 attctaaatt tactttgctc attaatttcc cctgtcgtaa tgttacgaac tgaaacggct
     4081 aaagattgtg taccagaatt tcctgacata ccactaataa taggaataaa cgctgcgagt
     4141 agcgctacat tttctaatgt tttttcgaat ctccctaaaa ttgtcgcagt aatcattcct
     4201 aaaaatgtta aaataatcaa ccatggtaaa cgttttaatg ctgttttaat gattgaatca
     4261 ttagtcgaat cgatatctga aaccccggct aaacgagagt agtcttcact agcctcttca
     4321 tccataacgt ctaaaatatc atcaatcgtg atgataccaa gcaaatgttc ttggtaatct
     4381 ataacaggta cagccatgaa atcatagtct ctcataactt gagcaacatc ttcttggtcg
     4441 tctgctacat tcacactaat gacacgttca ttcataatat cttcaatata agcatcattt
     4501 tcagctacaa ttaaatctct tagcgataaa acacctacta atttaccatc atcatcaacg
     4561 acaaagataa catagattgt ttctgcgtct ggcgcttgcg ctttgaccaa taataacgct
     4621 tctttaacag gcgtatgcgc tttaagtgat aaatactccg tcgtcataat accgccggcc
     4681 gtatcctcat catagtgaag taatgctttg atttcattcg cgtcatcttt attcatcaat
     4741 gttaaaagac tagcaacttt tggtttagtc aactcattta aaatatctac tgcattatcg
     4801 tatgacattt cttctaatat gtgactagcg tatgtcgcat tcatcttatc aaatagcaac
     4861 tcatattcat cgtcatcaat atctaattga tcaaagaaat ttgcaacctc ttcaggtgat
     4921 aaatattgaa aaatcttttg tctattttca tcggtagtat cttcaaaata ctcactttgt
     4981 tcatatgtgt gtaatgctag aaattcatct ctaaattgat caatatcatc attttctaag
     5041 tattgatcta ataatgtctc atcatataat tcctcttgaa cacgctcttt ttcatctgtg
     5101 ttcattgaca tacttacacc ccctgtgtga attagagttg attgaataat ttaagtaatt
     5161 gcttataatc aattgtaata taaatattgt tcttattgat tggatgaaca aaatatattt
     5221 gcgtacattg cagtacttga ccatgaattt tatcatgaaa accatcatac aaagaatctc
     5281 ccacaattgg atgcccaata tgttgaaaat gtacacgaat ttgatgtgta cgtcccgtat
     5341 gcaaatgaac tttgcataaa ctgtatttat cattctgatt tattacttca taagatgttt
     5401 tagcgtattt accatctgag gcaacttctc tagttataat actatccttt gaccgtctaa
     5461 tattagcttc aataatacca gatgtatggg ttttaccata tacaaggcaa gtatatattt
     5521 ttttcaagtt tcctttagaa aataaatgat ggatatgtcc atatttagcg aatattacta
     5581 taccagttgt attacgatct aaacgcgtaa caatatgtgg gttaatattt tcaccatgtt
     5641 cctgacaatg atatagtact tgttcgatta aactttcatg aggatgttct ctcgaaggcg
     5701 tacaattttg attgtttggt ttagtaacta tgatgataaa agcatcttca tacaatactt
     5761 ctaccttacg agcataaggt attaaattaa cactcggtat ttcttgcggt aaatgaattt
     5821 ctaatatatc atttggcatt aattgcttac gcactgtgac tggttcatca ttaacaatta
     5881 aagcgccatt atttttaatg gcgctcactg tcttcttaga aaaatcatgt cgtgctaaaa
     5941 aagttttaac agtttcttgt tgtgatatat gatacttaaa tttcatcgtt cttcatcact
     6001 tgatatgaaa gaatcgtgta cacgtttcca gaatgggaat ggtctaaaac gtgcaaacct
     6061 cactttttca tttgctacac ggtattgtat tgaattaaca tttttatgtt tgatactaac
     6121 atgatctatc gtcattctaa tggtatcatg attaactggt gatattaaac atgtatgatg
     6181 ctttggtaat acaagtggtg atcctaccgt tctaaacaca cgattattta ttgaggcaat
     6241 ttctgtaatt tgcattgctt caagtgaagg atgtatcagt gcgccaccta gcgctttgtt
     6301 ataagccgtt gaacccgaag gtgttgatac acataatcca tcgcctctaa atcgctcaaa
     6361 gtgtttccct cttaagttaa catccacaac aagtgttgag ccattttcag ttttcatcgt
     6421 tgcttcattt aatgctaaat accttgtttc atagccgttg tcgttgtatc tcataataat
     6481 ttcaagcaat ggatattcaa tgacctgaaa ctctgaatta ttaatttcga tgattaattt
     6541 ttcaacttca tgaggtaacc aatccgcata aaatcctaaa tgacctgtat gaactccaac
     6601 aaatgccact tttgataaca tgtggctata atgatggaat gcttgtagta atgtaccatc
     6661 accaccaact gaaataacaa tttcaggatt ttcactatcc tcaatcatgc gaaaatcttt
     6721 catatagttc atcattttat gctttaaggc attagacttg gagtcacctt tagttaaaat
     6781 tgtataacgc atgatttata acacctcgtt aatcattttc atgtttttta gaacgttttt
     6841 gcgtgtaata tttttgagct tcctgaattt catctttaat ttcagacatc tcttcatcaa
     6901 gtaaatacgc tgcttctgcc gctctttcca aacgatgttg aatttcatcc ggataagcac
     6961 catcatattt atatcgtaaa gtatgttcaa tcgttgccca gaaattcatt gctaatgtac
     7021 gaatctgaat ctcagccaat ataaattttt ggccttgtaa tgtttcgatt ggatattcaa
     7081 taatgacatg atacgagcgg taaccacttt ctttagtgtt acgaatataa tctcgttctt
     7141 caattacttt aaaatctttt ctttgtctta aaatatttac gacaacatca atatcttcaa
     7201 caaattggca catcattctt aaaccagcga tatcgtacat ttcttctctt aacctatcaa
     7261 atggtatttg tcgtttgttt gccttatcta taatactagc gattggttta acacgaccag
     7321 taacaaattc tattggcgac gcttgttcac caacttcata ctgtttgcgc atgcctttaa
     7381 gtttcacttt caactcatca accgcttgct tataaggtgt taagaactga tcccattgat
     7441 tcatagtgct tcaccccgct ttattcaatg tcgaatgttt cttcaacggt tgcaacaaat
     7501 tccttaccat aattattatt tccttttatg ttgtctaaaa tataagtaag ttcttggtgg
     7561 aattcagtca attctagttc atcattgata ataatttttt tagtcatgtt ttcatgcaac
     7621 atcgtccatg tttcttgtac gaaaatgaga taagcatacg ctttaccatc atctaatgta
     7681 tacgcaaagg cataattatc actatcaacg agcatttgtc ctacttcaga taatccttta
     7741 atagaatctt ctgtataaca ataaagtgtg tcatctttaa ttttaatttc attaatatat
     7801 aaacgcatgc ttaatcctcc tcttattcca taacatttta acacaattca ataatacaaa
     7861 acacttacga acactatttt taatggtcaa attaacattg ataatagata aaggaaatta
     7921 cttttaaaat tcaactttgt cattcataat atagttaaga taaattgaat ttgatacgag
     7981 gtgtatacat atcatggcaa caaatcatga aatagaattt aaacaaatga taactgcatc
     8041 aatctataat aaattgcaag agaaatattt taaagacagt gtgctgttta agcaagtcaa
     8101 ttactatatt gatacacccg atttcaaatt aaaggaacat cgctctgcac tacgcataag
     8161 agttaaagac aatcaatatg aaatgacttt aaagacacca gcaaaggttg gtctattaga
     8221 atacaactat atcgtcgaca ttaaaccaga gatgaacttg attatttcaa acgacaatct
     8281 tccagatgac attcgtcaaa ttatagttga acagtttggt gtaaaggata caactttatc
     8341 aatacttggc gcattaacga catatcgcca agaaactaaa tataaaggtg atttacttgt
     8401 tttagataaa agtgagtatt tcgatactac ggattatgag ttagagttcg aagttaaaga
     8461 ctataatcaa ggtttacaaa agttccaatc tttattaaac gaactaaacc tcgaacatca
     8521 tcaaccttta aataaagttc aacgtttttt taagaaaaaa gaaacacttt caaacaatat
     8581 aaattaaatt ggcaaatatg catatttctt tcaaatgttt aaaatcatgt tatattagtt
     8641 atatattaac ttaagttcgt aacagtaaat ttttgaaaat gggtgattaa atgacaacaa
     8701 caccatatga catcattggt aaagaagcgt tatacgatat gattgattat ttttacaccc
     8761 ttgtagaaaa agatgaacga cttaatcacc tgtttccagg agattttgca gaaacaagtc
     8821 gtaaacaaaa gcaattttta acacagtttt tgggcggtcc aaacatttat accgaagaac
     8881 acggacatcc tatgctaaga aaaagacata tggcgtttac aatcactgaa tttgaaagag
     8941 atgcatggtt agaaaatatg cagacagcaa ttaatcgcgc tgcgtttcca caaggtgttg
     9001 gagattattt atttgagcgc ttaagattaa ctgctaatca tatggtgaat tcctaaaatt
     9061 aattgtaggt gaataaacat ggctggagaa ttacgaataa tggaaaataa gagtcgtgaa
     9121 gatattaatc tatcacctgt aagtaaaatc gaaatttatt catttttcga tccatttagc
     9181 tccgattgct tcaaattatc agcaatctta tccaaattaa gaattgaata taatcaatat
     9241 atacgtatca gacatatatt aaatccttcg ttaaaagtat taacgaaatg ccaagctcaa
     9301 agtacatcca actttgataa catcgcccta gcttataaag cagctgagtt acaaggtcgt
     9361 gtacgtgccg aacgatttat acatttaatg caaaatgaaa tcatacctaa aagagatatt
     9421 attacagaat caatgatttg tgactgtatt caaaatgcag gtattgattt agaagtattt
     9481 aaagacgact tacaaaaaag taaactaact gaaagcttga aaattgattt gcatattgca
     9541 agagaaatgg aaatcgaaca agccccttct ctcgttttct ttagtgaaga tgttcatgaa
     9601 gaaggtttaa aagtcgaagg attataccca tatcacatct atacttatat aattaatgaa
     9661 ttgatgggta aacctatcga aaagaatctt cctcctaaac tagaaactta tatacagcaa
     9721 caacaacttg taacgatgga agaattactt actatttatg aatggccaga aaaactttta
     9781 aacaaagagt taaagaagtt aaccattcaa caaaaaattg aaaaacttaa atatccagat
     9841 ggcgatttct ggaaatctaa aatgcctaaa atcaaatcaa aataaataaa ttaaagacct
     9901 cgtacaccat aaatgtaacg aggtcttttt attgactctt aattttgcaa cattaaatta
     9961 ttagaacgta atgttacgca tcgatctaaa tatattagtg atgtttttcg aatttaacca
    10021 gttcgaaaat tatacaaatt atgaatttgg tacataatag acatatatta atcgtcaatg
    10081 tataaaaagt gtatatttaa tacattttta tacaacttca cttccctctt attaaaaaag
    10141 aacataaaaa aacgtcgtgt ctgaatacac gacgctaaac aaaggggatg ggagaaattt
    10201 ttcacttcaa acaaaggggt atgtttgtta tgtgattaat ttcatgttca taatataaca
    10261 cgctatctct cccatttcaa ccttttaata gtacttaatt taaattgtca caatctgttc
    10321 ataatgtaag cgaatacaat agtgctaagc tttcattaat ttttcaaaag cgttcaattt
    10381 ttgttcaaaa acttcacaag cttgttcaat tggttcaggt gttgtcatat ctacaccagc
    10441 attttttaat atctcaattg gataatttga gctacctttt ttcaagaatt cattaatata
    10501 tctatctact gctggcttac cttctgttaa aatttgatga cttaagcttt gagctgcact
    10561 gtaaccagtt gcgtattgat atacataata attcatatag aagtgtggaa tacgtgacca
    10621 ttccttacta atatcttcat cagtttctac agaatcaccg aagtataatt tatttaattt
    10681 ggcatattct tcattcattc tagttggcgt taatggttca cctgcttctt caatggtatg
    10741 aattttatgc tcaaattctg cgaacattgt ttgtcggaat aatgtagctc tgaaacgttc
    10801 taattcttgg tttaataata ataagcgttt ttcatcatca agatgtttat ccatataatc
    10861 acttaaaagt gcttcgttac aagttgatgc aacttcagcg acaaaaatag tgtagtcact
    10921 agaatttgac ggttggaatt ttctactgaa gtaactatgt gctgaatgtc caaattcatg
    10981 aactaatgtg tataagtctg aaatagtatt agaccagtta agtagaataa atggattagt
    11041 taaatgtgca cctgatgaat agccacctga acgtttacct ttattctcat agacatcgac
    11101 ccaacgattg tttaagcctt ctttaactac atttaaatat tcttcaccca ttggttctaa
    11161 agcttttaac atccactctt tagcttcttc ataaggcatt tcaaacttaa tatctttaat
    11221 taatggtgta tataaatcat acattttcaa gtcatctaaa cctagcaatt ctttgcgcaa
    11281 ttcagtatat ctatgtagca atggtaaata tttatgtaca gtttttacta gattgtcata
    11341 tacattttct ggaatatgat tattactcaa tgctttttct cttgcagttt tgtaattgtg
    11401 tgtacgagca ttaaatacat ttttcttcac ttcacctgct agcgtagcgc caagcgtatt
    11461 attatgagct ccatatgctt tatatacatt tctataagca ctctctctta gtttgcgatc
    11521 atctgattct aaatacttaa taaatgtacc ttgtgttaac gggtgtgcat ttccatcttt
    11581 atcaatcgca tcttcaaata ctaaatcagc gttgctaaac ataccgtata catttgatgg
    11641 cgttgataac gcgtcctgcg cttctgttaa taacttttca gtttcagcat ctaaaatatg
    11701 aggacgtttt tcattaatca acttcaaatc aaatgcgaat ttttgtaatt tatcatatga
    11761 atttacaaat gattgaattt tatcttcatc aatttgtaaa atctctggca ctaagaaact
    11821 ccatgccgag ctaaatttaa taattaattg atgtgctctt gactccatac cagtatactt
    11881 gtcgttcgtt gtatcttggt cttgttttag gtgcgcgtat acatatactt tttctaattt
    11941 agtacctaat gtatcttcta attctaacgc attgtataat gtctcagcac tatcaccaat
    12001 gtgtccttta aattgttctt ctttacctaa ctcactttca acttctttaa atgcagcttc
    12061 aaatgcttcg tcatctttaa agattgtagt taagtcccat gtatattcag gatatttacg
    12121 ttcctgttct tctctcgtta attgttgact catatgtagt aaacctccta ataaaatata
    12181 attgatataa ttttctcatt ttagaacaaa atttgcacgt cattacctct atctgaacta
    12241 atatttaaat aattgttaat aagtttttct ataattgctg ttttactttc tagagcaaca
    12301 ataaactttt tcagtttaaa ataattaaga ttgtcactta ctaatttgct ttttccattc
    12361 tttaaccata aatcaacttg taattgccac tcaattggat gattttcaat ataaatttgc
    12421 tctggaaaaa tataaccata attgtgcact acttcttgat cagttagctg taattgatac
    12481 attgcactta aagtgggttc taaaacagaa ttttgccagc gacaatattt aatatattga
    12541 ttaattgcga actttgataa tttataaaca gcatgataat cacaaggcgc ctcattaaac
    12601 aactcactaa ttttggcatt ccttttttca gcgacatatt tgtgcccgcc tatatattgt
    12661 atttgttgaa acatcattat ttgttttttc tctaaattga acgtatgaag ttttcgagtg
    12721 attggattaa taaacatact ttgaaaatga ttgaacttca ctttattatg acaataatca
    12781 acatcattta ataaccaact tactttatat cccattttct ttaaaccttc cgttcgttga
    12841 agcatctgtt tataaggaat tggcgaatac tgtagctcaa taacatattt attattaatc
    12901 aaaatatccg gaatttgttt tatttctttt aaaaatggct ctatctctac tttacatcct
    12961 tgttgctgta tcatctgtgc taaaatcaat ttacttttat aatgtttaat cgtttcatta
    13021 ttaaaacatt tttgttccgc taaatgttta tgtgcaaaat gactaattac tttgagccca
    13081 cgctttaaaa taacttgctt gccacacacc ggacaaaaat attgtgtctt tctcaatgca
    13141 gtagttgcta acacacgttc cttttcttca tttaaagcta ctaacatatc ttcacctcaa
    13201 aatattacac gtaagtttaa ctactattac tttttcagct tttgaaatca ataaaaaaag
    13261 agactccaaa gagtctcctt tcatagtgcg ttgcttaatt aatatcgtta ttcagttgtc
    13321 tctggaaaat aacgtcgaac ttgagctgtt acgttatgac tcataattat tttagcatag
    13381 tcatttaaat aaacttctgt tctgtctgtt ggataagcaa attcaagcaa ttgactgtaa
    13441 ctatcattaa tgacctcttg atcaacatga ctatcaaaat gtacagcata ataataagta
    13501 ccatcaacca tatataacaa atcttcaaac tctgtagtta atggattgct atgatatgca
    13561 taattaataa catcttctaa atcgttaaat ttcacaatga ttgttcttgt atttttacgt
    13621 gctgaagact tttgacgttt agaaccttgt gcctcttttt cttttgttcg ttgctcgaat
    13681 aattcttcta attgatcttc accttctaat gtttgagcta acaattcttg aacttgttca
    13741 tcaaattgat cagttgcatc gtcatcagac atattcatca tatcttcatt tttagattta
    13801 gaaattgtaa cttcgacacc tttttcaaag gcatgtactt gaatccataa tggaccttct
    13861 acaacaaaat cttcttcttc gttaatttca tccatcattg accaaaagaa ttcttcgcca
    13921 cgtttgcgat ttgtccataa atcttcacga ctaaacccac gggcctcgat atcgctatat
    13981 gttataaaca attttacagt tgtatcatct actcgttcta ttctcatatc atctcactcc
    14041 ttacagtcga tgaatagtat ccacattgta ttaaagcatc aaacaaaata caatatattt
    14101 gtttgatttc
//
LOCUS       DAHHRR010000066        13816 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.066, whole genome shotgun sequence.
ACCESSION   DAHHRR010000066 DAHHRR010000000
VERSION     DAHHRR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.066"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..185)
                     /locus_tag="NBI42_001652"
     CDS             complement(<1..185)
                     /locus_tag="NBI42_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778069.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFI"
     gene            complement(182..388)
                     /locus_tag="NBI42_001653"
     CDS             complement(182..388)
                     /locus_tag="NBI42_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCD1778070.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAERKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            complement(425..961)
                     /locus_tag="NBI42_001654"
     CDS             complement(425..961)
                     /locus_tag="NBI42_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCD1778071.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            complement(954..1202)
                     /locus_tag="NBI42_001655"
     CDS             complement(954..1202)
                     /locus_tag="NBI42_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCD1778072.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEDQEND"
     gene            complement(1217..1459)
                     /locus_tag="NBI42_001656"
     CDS             complement(1217..1459)
                     /locus_tag="NBI42_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778073.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            complement(1474..1674)
                     /locus_tag="NBI42_001657"
     CDS             complement(1474..1674)
                     /locus_tag="NBI42_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778074.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            complement(1677..1934)
                     /locus_tag="NBI42_001658"
     CDS             complement(1677..1934)
                     /locus_tag="NBI42_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCD1778075.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            complement(1934..2335)
                     /locus_tag="NBI42_001659"
     CDS             complement(1934..2335)
                     /locus_tag="NBI42_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HCD1778076.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            complement(2335..2520)
                     /locus_tag="NBI42_001660"
     CDS             complement(2335..2520)
                     /locus_tag="NBI42_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCD1778077.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            complement(2533..4485)
                     /locus_tag="NBI42_001661"
     CDS             complement(2533..4485)
                     /locus_tag="NBI42_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HCD1778078.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            complement(4553..5110)
                     /locus_tag="NBI42_001662"
     CDS             complement(4553..5110)
                     /locus_tag="NBI42_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HCD1778079.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            complement(5136..6302)
                     /locus_tag="NBI42_001663"
     CDS             complement(5136..6302)
                     /locus_tag="NBI42_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HCD1778080.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYEIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            complement(6299..6661)
                     /locus_tag="NBI42_001664"
     CDS             complement(6299..6661)
                     /locus_tag="NBI42_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778081.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            complement(6675..6935)
                     /locus_tag="NBI42_001665"
     CDS             complement(6675..6935)
                     /locus_tag="NBI42_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCD1778082.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            complement(6940..7242)
                     /locus_tag="NBI42_001666"
     CDS             complement(6940..7242)
                     /locus_tag="NBI42_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCD1778083.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            complement(7235..7552)
                     /locus_tag="NBI42_001667"
     CDS             complement(7235..7552)
                     /locus_tag="NBI42_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778084.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            complement(7645..7806)
                     /locus_tag="NBI42_001668"
     CDS             complement(7645..7806)
                     /locus_tag="NBI42_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCD1778085.1"
                     /translation="MSDTYKSYLIAVLCFTVLAIVLMPLLYFTTAWSIAGFASIATFI
                     YYKEYFYEE"
     gene            complement(7818..8081)
                     /locus_tag="NBI42_001669"
     CDS             complement(7818..8081)
                     /locus_tag="NBI42_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1778086.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            complement(8106..8321)
                     /locus_tag="NBI42_001670"
     CDS             complement(8106..8321)
                     /locus_tag="NBI42_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778087.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            8368..8607
                     /locus_tag="NBI42_001671"
     CDS             8368..8607
                     /locus_tag="NBI42_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778088.1"
                     /translation="MISIHKRLLTQYLDKEIVTSLDLHLINGEVIKVQEHIKDAESKT
                     LHIIHPKDRVVSLDHVLYFDINVKGEKNNESPYPS"
     gene            complement(8604..8780)
                     /locus_tag="NBI42_001672"
     CDS             complement(8604..8780)
                     /locus_tag="NBI42_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778089.1"
                     /translation="MNEEKLKMILLLLEDIPRDEWNRLVNEVNNQYSYQADKVGLASD
                     NCQQIANNYKHYGF"
     gene            complement(8864..9055)
                     /locus_tag="NBI42_001673"
     CDS             complement(8864..9055)
                     /locus_tag="NBI42_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1778090.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNAKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            9216..9545
                     /locus_tag="NBI42_001674"
     CDS             9216..9545
                     /locus_tag="NBI42_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1778091.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            9558..10019
                     /locus_tag="NBI42_001675"
     CDS             9558..10019
                     /locus_tag="NBI42_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCD1778092.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEELAHHKLTYGNILDQTKWINRKFENYARRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            10037..10471
                     /locus_tag="NBI42_001676"
     CDS             10037..10471
                     /locus_tag="NBI42_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778093.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQKHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            10500..10895
                     /locus_tag="NBI42_001677"
     CDS             10500..10895
                     /locus_tag="NBI42_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778094.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDRSYPMSKINDVNITGKVVRLFRDFK"
     gene            10985..11131
                     /locus_tag="NBI42_001678"
     CDS             10985..11131
                     /locus_tag="NBI42_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778095.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(11128..11742)
                     /locus_tag="NBI42_001679"
     CDS             complement(11128..11742)
                     /locus_tag="NBI42_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCD1778096.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLLIFS
                     IETCLIWFYSFIIFNNVDLKNLNLIQLLTGLKANILFLFIFVLTVFVFNPLIVKFIIW
                     LINITRKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNSAGSDF
                     DLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
     gene            11868..13073
                     /locus_tag="NBI42_001680"
     CDS             11868..13073
                     /locus_tag="NBI42_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1778097.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            complement(13116..>13816)
                     /locus_tag="NBI42_001681"
     CDS             complement(13116..>13816)
                     /locus_tag="NBI42_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778098.1"
                     /translation="ILEIFKNIDHDFRDGVSEEMMKHLKVVKQNIERIKNQNDIYGRQ
                     IAEIRSIMKQQDATILDGNFQINCSGENMVQGLVIPSNYLGRKMKILKDHIDDGIKKI
                     ADYVQGIYDEYASKIVDVIKYLINTIPKIRKNLRHAIEMLNVKKKEFLSLIPNVTCNY
                     IKTKLEELDNTLGKWEPFLNDLKAVSPILENHLDDIVKNMKPLIVQMIFEPSHYDDMF
                     ISRKALTPVFSSVL"
BASE COUNT         4397 a         2540 c         1939 g         4940 t
ORIGIN      
        1 ttaataaatg taaatgattc aatctcatct cttttaaccc atacttcatt gttgaacaca
       61 tctttgaccg gaagaaaatc ctcaatcact agattcataa caagattaat ataatcgtca
      121 gaagctagat ctgttgttgt gtaataaact ctatctgaaa tagttttaat tttaacctcc
      181 gtcatttccc acactccctt atattttcaa ataactgacc cactttaata actgcatctc
      241 ttttaacttg tgcctcgtat ttgcgctcag cttcttcttt actctctgcc tcaacaactg
      301 taaacctttg attgctttta gctttagtta tgtgtgtatg cttgcgtcct gttgaatctt
      361 tgaatgttgt gactaagtat tgcgtcactt ccccaaaacc tccttgactc gatctaagat
      421 gtctttacac gtatcctttt cctgcgtctg ctgttccatc ttgtctttca tgattccttt
      481 tcattttctt tttgtatgcg tcaatgagct ggtcgataga atataagttg aaagctatgt
      541 ctatcgctat tacaattgcc aattggtcag gataaaattc tttgaatatt atctgtggtg
      601 tgctaacaac tgcgtcttga gcaaattctt tatctttaaa attaaacatg ttgtgaaatt
      661 cactattttt aaaacttgat tcaatcgctt cttttatctc ttctgatgac actcctactt
      721 gattcgcaat actcaatcca aacgccaaca tgtccgctaa ttcatctaac tgaacatcta
      781 acggcttacc tggtttcttc ttccagttct taaacgtttc caatgtatta aaccattcaa
      841 agaattcaac tacatatgct attttgctat ctcgtaagtt cagcgttggt attctatcgt
      901 cgaactcctt ttgtatttgt aataactctt gtaactgatc aattgttaat gtattagtca
      961 ttttcctgat cctcctcata tttatagacc acttgccccg tcataatccc tactgcttca
     1021 tcaagaccaa tatcttcttt gagtgcatct tgcatagcat taggtaaacc ttcaagtatt
     1081 tcatcaaacg cttgcgcttt cttatacacg tcttcaatct cttttagtaa tccctctgtg
     1141 tcattgccgt tatacgcact agcactgata attgattgtt caatttgttc gcggttattc
     1201 atcatttcca tctcctctaa aataaagtta gttgcttctg ttcctcgtat tccaaaccat
     1261 gttgctttat atatatttcg agctcttcag cagtatcaaa tgtcttttta acgctttgcc
     1321 aacctggcac gatatgccca tgaaagtaat acgtgccatt tactacatga gtatgtgcca
     1381 ctcgttcgtt atcctgatac agatatcttt tagagccgaa aaattggttt aagcgttctt
     1441 tacgtgcgct atctgtcatg gtcgtcactc cttttaacaa ttaggcagtc caaacgacat
     1501 gcattcgtaa tatagttctt cattccttat gcttgtctta tagttctcaa tcacattgct
     1561 aacttcttta tgactcattg ctttaacttg ttcgtctgta tatttttcgc agtcttctaa
     1621 ttccagttgc tcctgtaatg acattacgta atcaacttgt ttttgcgttg ccatcgttac
     1681 ccctcccaca agtcaaacac tctatcgacg taaaacttcg cctttgctaa atcctcatga
     1741 ccattcttta acggtgctct agacaagtat ttgattgcat tacctattgc gaatgctaat
     1801 tgtggtggat actgtgccgt aacttgttcg atgaaatcta taatttcaat gtcgccgtat
     1861 gtgtaatgcg caggttgttt aacattgtct tgtgtttcat tcatatctac ttttctgtta
     1921 ctgattatgc tcattatgct tcactccatt tcttgaacat ttggttataa gtgacatcga
     1981 accagtacgg atcacgtgaa tgtttttgag gcgttccatc ataaagccat ggtctcaatc
     2041 ttctctttct ttcttcttca tattccgctc tcacatttcg ttggtatagg ttcaaaatcg
     2101 ctttttttct gattttttct ctctcttttt cttcatcttt tatttgactc ttcatatatt
     2161 caacttcatc tttagatttt gagtcttttc ttccacacaa taattcatcg ccgcgcattt
     2221 tatgtttgta tctgtatcta agaagttctg gagatatatg atatttttct gaaacttctc
     2281 tcaatgtcat tagttttcct ttgatacgca ctcttataac ttttcttcta gccatcattc
     2341 cacctctaaa tctaaaacct tgatatttat aacgttatat tttaatagtt cacctggatt
     2401 attaaataaa tagtccgcca aattctcttt ttctttatca atctgattgt aattaacact
     2461 ttcgacttct gtaggaattc taatgtcaac agaagcattg atataagctt gatgttgcat
     2521 tcaatcacac tcctaatcct tcatataaaa cggagaagta aatccgtcac tattcaaatt
     2581 caatcctttt gcccaatcga caggcttatt catgatagtt tcgatttcct taagtccatt
     2641 tgaacctcta ggtatttcta caattacttc atcatggaca tgtccaacta ttttaaaacc
     2701 tgatgcttca agcctagcta tagaaatcgc aagtaaatcc cttgcagttg cttgaacaat
     2761 attctcgact aacttcccac catacgtttt taactttgac catttacggt taagatctaa
     2821 gcccataaat tcaacaactt gactacccca actattttca ccaactgaag cttttggata
     2881 agctaaagct cttccactag gcagttcaat cattagaaaa ccttttttca tataaaatct
     2941 aactccatgc gtatgatgcg tctttcggga tttcacagta ttaattgcag cctcttggca
     3001 agccttccaa aaattaacta tgttaggatt tgcgttacgc caactatcaa ctaaaccttg
     3061 taattcattt tcttcaatgc ccatttccaa tgcgcccatc gcttttaaag ctccagcgcc
     3121 accttgataa cctaaagcta attcggacac ttttcccttt tgtctgagag ggtcgccttt
     3181 agttatgctt tctaccggga cattaaacat ttgagaagcc gatgcttcat atatctttcc
     3241 gtgtgtgttg aacacatcta aacgccattg ttcttttgca taccatgcta tgactcttgc
     3301 ctctattgca gaaaaatcac ttactgctag ttcattacct tcttcagcag taaatgtcgt
     3361 cctaactaat tgacttaata agtcttgagg atgaacattg agtaataaat ctaaatcgtc
     3421 aaaacgttgt tctttaataa gatctcttgc tatttctaat tcagtatctg aaatataatg
     3481 ctttgttaaa ttctgaagtt gtacacctct acctgcccat cttccagtac cggcaccgta
     3541 aaattgaaac agacctctta cccgttcatc actgcacatc atgtcatgca ttttgttgta
     3601 ttttttcaca ctggttttag acatttgcaa tctaatttct agcatttttt tagcttttcc
     3661 tgttgcttct tttaagtact cctgaaccgt tttcttttgt aaattaggta tatctaatcc
     3721 ttggtcatcc tttaaccaag ccaataattg tgtaggacta ttaggatttt ctaaacctgt
     3781 tatatgttta gcttgattaa gcaattcttc tttactctgc ttatcgagca cattagctcc
     3841 taacatcaat gatttagaaa gcttaatacc tctgtcgttt atatgttggt caaaaaccca
     3901 atatgcttgt tcaattgcag ttactggaaa gtctttaatt ttatgagcaa tcgtcatttc
     3961 tacttctaca tctcgaatac agtaatctat aaattgttgc catttttcaa gatcatgttc
     4021 aggcaagttt cttgttcttc ctccattaac ttttgttggt ttacaaggta tagagaaata
     4081 acgaattaaa tttttacctg ctttatcttt ttggttttgt agtcttaaaa cttctccaac
     4141 tttatcaagc gaagcaggta agccaatacg cattgaatta accattgtgc aaatccattc
     4201 ttcaggtggc atctgtttat taaaatgttt agcaagacaa gttctttcga aattagcatt
     4261 gaatgcatac ttttttacag caggatcaaa aagagcaatt ttaaacgtct caaaatcagc
     4321 gtggaaaggc tcattatcta ctttagtcat gtcaatcgca ctaatcgctc caccatctat
     4381 tgaataagct ataattaaaa tttcgaaatc ttcagcttct gtgtatttat aggcaccaca
     4441 tttcgaaata tcattactgc tatatgtttc aatatctata ttcataaatc tcaaattctt
     4501 gacacctcaa tttctttaaa attaaagtgg ggctaaaacc ccacctattg acttataaga
     4561 aatcctcatc atcagtgtct aattcatcga aatcatcttc tgctgcactt gcaccgccaa
     4621 gaggttcgcc tttttctaca agttgaatgt tgttcaatcc aactgcgata cccttattac
     4681 catttgtgtt gaaaggaaat agattaattg aagctctaat ataatcacca cttacaacag
     4741 ttccagaatc cgttaatcta attttgtttt ggtcaataat accaggtgct tgtttgcttg
     4801 atgcgttaat aaaataagcg tcttgataat tgacatcatc ttctctttca gtatctccat
     4861 cacgtaatgg aagtttcaga tttgcaggaa ctttgcctcc aaacttacta acttttcctt
     4921 cttctttagc agcttctata gcttgttcaa tggcttttat cgtacttgta tctgatttag
     4981 gaatgattaa actgattgaa tactttgatt cttgcccttc ttgcatactg tgaggttcaa
     5041 aaatatgtgc atatgatgct cttacttttc ctgtaatcac tttagtttta tttaatactt
     5101 ttgctttcat gtttatatac cgtcctttta aatttttata gtttgtcaaa atcatcttca
     5161 gcagattgct ttatagctgg tcgtttatcc gactcggtag caagtgttaa tttaccttgc
     5221 ggcttttcta taaaaccctc tgtaatttta gaaaatgctt ttttaccaat taatttttct
     5281 aaattcgtaa tgctaagtaa cttggtttct gtaatatctt caggtttata acccgcttca
     5341 actaactttt caagcattgc ttttgtatca gttatcattc ttcgtgaacg accttctaca
     5401 agcttccaac caggatagtt tttatcattt tctttcgctt gatttagtgc atattgttct
     5461 acttcatcag cccatttttt gatatcaggc agtttatata aaagttctgc aatctcttca
     5521 tcacttaaca aatgtggtgg cttttgaggc acattttgca tgtattctgc acgtgttcta
     5581 catgaatgat ttatcttaca gaatctacaa tgactacctg ctttaaactc tcctccaccg
     5641 ttataagcaa gtctggctaa tggtttaaca aaatcggctc cccattgaag taatcttgat
     5701 attggtaact cttcagtaga aaagttatct attcgaggtt gtatgatagt catgcgaatt
     5761 gtatgaattt catacattaa actaagcagt tcatatgcgc ccaagccata taatctaagt
     5821 tgaggattat ctatagctga aacttcaatg cctttaccat atttaaggtc aataatttca
     5881 agtacaccac ctgaaaatat aatgacatca ccagttccaa aagattcagg gacgtattta
     5941 cctaaatcta attttgtttc aaataaagct attacatcat tatccctact caaagcttcg
     6001 ttatattttt cttctacatt agctacatac tcttccacat attcacgcaa ttcttcgctg
     6061 taatattgat ttctcttata attttgaaaa gccttattaa actcaaactg tgttaggcct
     6121 tcatatttaa ggctgaaata taactcactt aactcatggg cgaatgtacc ttcttcggca
     6181 aaaactgtac ttttatctgc aataccttca cttgccttaa tactcggtgg gcagtttagc
     6241 cattgttttg ctccacttgc actaagcttt gcatgagctc tatttgagtg atctagcttc
     6301 atgcatttat tctcgcattc ataaaatcaa caattttttc ataatgctct tctttgatag
     6361 tagatagctt atccgcacca agttcgttaa gtttatttct aaattctttc ttatcagaag
     6421 tatctgcttt tttaaggaac tcttttccta ctgataaaac ataatcttta gttaaatcag
     6481 cagaagtttc cttaacttct tcaattgatt ccagttgagc tgtttcttct tttggcattg
     6541 gtgcttcttt aactttctct tgtacaattg atgaatctac tgttgatagt tcagtgttta
     6601 acacacgtaa attgttattt aatagtttta attcttcaaa aatatcttct aatattgcca
     6661 ttgattaaat cctcctacca tttcatgact aagttaatta gtctgtcctg ttcatctgtg
     6721 ttctcttcaa tccattcgtt tataacgtca cgcattgcat ccgtcgcaaa atatagttcg
     6781 cttaaatcta caacatgaaa tgatttaagt ggaatattat tcatatcctt gatttgtata
     6841 ctgataccgt catgtttttt catcgcagac actttaaatt caaacccgtt aaagctgata
     6901 attttatttt ttatctcacc aaatttgtaa tacattgttt tagccctcct tgttatcatc
     6961 aataccgtga aatttttgtg atttacacat ttggagaaca ttgacaatgt ctttataact
     7021 cttagtgcta tccaataagg aagcaagatc gaaagtatga ccaatcacag aacttgaacc
     7081 tgctaaataa tctccgtcga taactcctat tgatgagaaa agcaaaatat caaatttact
     7141 ttctccctta atttctttcg ctaattcata caattctccg cttttttcag ataataagtc
     7201 ttttacttct tcctgagtca tgtctttata ttttttagtc attgttgact tcctcctcgt
     7261 tttcttcgtc ctcctcgtta tcttcttcgt tttgtaattc ataaattttg ttttttagtt
     7321 ttatattttc tttttccaat ttttcgtttt ttctttcttc cgcaaaatac ttacctctgt
     7381 aagtatcttc ttctttatct ttaacagcct ttatttcaat aagttttctg tactcgttca
     7441 atgtgattgt tactgtcaat tcttgatttg ctacaaagtt atcttcttca tttctgtatc
     7501 ctgagaaatc tttagtgtaa taatgttgtt cagttttaat attttcagcc atagttgact
     7561 acctccgtat attttgattt aattaagttg tatattttga taaatgtttg tcactgttac
     7621 ttgttgacgc aagtagcagt ttttttattc ttcataaaag tattctttgt agtatatgaa
     7681 tgttgcgata cttgcgaatc ccgcaattga ccatgctgta gtgaagtaca gcaatggcat
     7741 aagcacaatc gctaagactg tgaagcacag tactgctatt aagtagcttt tatatgtgtc
     7801 gctcatttaa tatcctccta ataccatttt ttatgctttc tgatcaaata ctcttccaat
     7861 ttagaaatat taatcagagt gcctgttggt gaataatcaa tgtataaatt ttctacacct
     7921 aaattatctt tgcggtaata tttcaaccag ttgtatactg tacttctaca tactccaaac
     7981 aattgatgga tttgtgtagg tgttgcgtat aactttttca caaatttttc ttcgcctcta
     8041 tatgtgtttt ctggtgttgg tggtactatg atttttggca tctctatcac tcctttcgat
     8101 aaatgttaaa gtttgttatt attcgctctg tattgaagtt ctctatctaa tgcatagaaa
     8161 actttgttta tttctaagta gctgtaatca ccttttttaa tacttcctaa tatttccttt
     8221 cttagtcgac gttcattttc tgttaaagat tctactgacg catgatctct tctgaaaatc
     8281 cttggtatga ttatgtctaa cccttctgat tttttgttca tttgttgttc cacctttcgt
     8341 gtataatgtt gttatcaacc taaggaggtg ataagtatac ataagagatt gctcactcaa
     8401 tatttagata aagaaatcgt tacttcttta gatttacatt taattaatgg tgaagttatt
     8461 aaagtacaag aacatataaa agatgctgaa agcaaaactc tacacatcat tcatccaaaa
     8521 gatagagttg tcagtttaga tcatgttttg tatttcgaca ttaacgttaa aggtgaaaag
     8581 aataacgaaa gtccttatcc atcttaaaat ccatagtgct tatagttatt tgctatttgt
     8641 tgacagttat cactggcaag tcctaccttg tcagcttggt aactgtattg attatttact
     8701 tcatttacta atcgattcca ttcgtctctt ggtatatctt caagaagtaa gagaatcatc
     8761 tttaattttt cttcgttcat ttgttgttcc tccttttaag ttgtttatgt ccatttgtta
     8821 cattacctaa aagttataaa aagttatacg aaggtcaaaa aatttagacc tttatctggt
     8881 ctacctcaat atcatataac tttgctaaag cgtaaactgt aacattactt aaattagtgt
     8941 catccttttc ccaatgacca actgtctttg cattgacgcc caatatatct gctacttctt
     9001 gctgtttcaa gtcccttaca agtctccatt ttcttacgga taattgttcc ggcatgtcgt
     9061 ccacctccct ttcacacgta taactttata ttactttgta ttacttgtca acagataaag
     9121 ttatatttct tcataaaaag ttataaaaag tattgtaaag taatgtcggt tatggtaagt
     9181 taatattaca ttaagtaata ttaaaggaga caactatgga gaataataaa gtcagaaaaa
     9241 ttttatctga aaaccttcaa gaacttatga atgataaaaa tattgatcag agagaacttg
     9301 ctgaagctat tggagtttct caacctacag tctccaattg gattcaacaa actaaatatc
     9361 cacgaattaa aagaattcaa caacttgcag attacttcaa tgtaccgaaa tcaagaatta
     9421 ctgaatcaaa aaaagatata catcaagaaa caattgctgc tcattttgat aaggagggat
     9481 taactgaaga agagattgaa gaagtaaata gattcattga atgggttaga aatagagaca
     9541 aataaagggt gtttatattg ggattatacg aagaactttg cataaataac gaaaaaataa
     9601 agatagaaga aactgaccag cttccaaatt tccaacctgg atgctatatg aacggaaaaa
     9661 tttatataag gcgtaattta tcagaagtac gtaaagcaga agtgttatat gaggaacttg
     9721 cccaccacaa gttgacgtat ggcaacattt tagaccaaac aaaatggatt aatagaaaat
     9781 ttgaaaatta tgcacgtaga catggtttta cttcagctgt accgctacat gaaattgtag
     9841 aagctcacaa ctacggtgtt cgtaatttgt acgagttgtc agagtatctg cagttgagtg
     9901 aatcatacat actagaagct atagaacaat ataaaaagat atatggtatt ggaactcact
     9961 atggcgagta ttctattacg tttgagccgt tgagagtttt taaatataag gaaatataaa
    10021 caaaggagag atacatatga aaaaagtaat cggactgcta ctagtaagta cattagcttt
    10081 aacagcttgt ggtgaaaaag aaaaaccaaa aaaagaagaa aataaaaagt cacaaacaca
    10141 aaaacacaaa gatagcaaac caaaaacgca acaagaaaaa atgaaaaaag ttgaagataa
    10201 aaatccacct aataatagca tacaaaataa ttcaaacaat caaaaccaat cacaaaacaa
    10261 tcaacttaat aataattcag atccatctaa taatactcct gcaaatataa atgaaaacga
    10321 ttcacaaaat actaatttaa atgatgagta tgtcgtttcg cctggctgga ctaaagatga
    10381 acaggctaaa gcttttgaag agtacaaaaa aggaaaagaa gaggaagcaa gagctggtgc
    10441 tagcgcagta ccaggagcca atattaacta ataaaacaat ataagaaaga agagctaata
    10501 tggaaacaaa taaaacaatc gatttaatga attatgtgga atttccaaaa agatacacag
    10561 aggcaaaagg caaattagtt gcacaaccaa taactactat aaatagcgca agaagagttg
    10621 aaaatgaaga tatgactgtt tgctacattt tagatcagga tgatgatgta atggacttta
    10681 tctttgacag agatataatt actgtttact gtcctgaaaa cggaactgcg actgatgaat
    10741 atttttgtga aattatattt aactcagatg acacatttac cctaaagcga ttatctaatt
    10801 acgttaccat taaagataga agctacccaa tgtcaaaaat aaatgacgta aacattacgg
    10861 gcaaagtcgt cagattattt agagatttta aataaacttg gctttaatta cgattaaaag
    10921 tacctatata gcgtgacgag aaaaaggatt aaaaaaaatc aaaaaacgcc tactagtgta
    10981 gacgttgaat ggtggtgaga attttatggt agataaaaac aaaaaacaag aaactactcg
    11041 tagtaaccca ttaaacaaaa gttttgaaaa gtcaggcgcc agcgaaaaat taaaaagcac
    11101 tttatcagaa aaagctaaga aaaaagatta gtattcattc attaaatata aatccaattt
    11161 aatttgttgt ttaaggtcta caagcgtatg tttaatatac aattcatcgt ttgacggtaa
    11221 atcagatact ttgaaatctt gtcgctcaac ttctagtaaa tcgaaatcgc taccagctga
    11281 attataggtt ttaagttcac cctcttcaat gattctgttt tcaaagtctt ttataactat
    11341 aaatactggt ttaccgttgt tattaaacaa cttgtctctt ttgtctaata agcttataca
    11401 atccaatttc ataaactttc tggttatatt aattaaccag ataataaatt taacaattaa
    11461 aggattaaat acaaacactg ttaaaacaaa aataaataga aacaaaatat ttgcttttag
    11521 acctgtaagc aactgaatta aattcaaatt ttttaaatca acattattaa aaattataaa
    11581 actataaaac catatcaaac atgtttcaat agaaaaaatc aataatacag gagtattgat
    11641 aaccttgttt ttttcactaa ctaaacctat cattgttaga tatttatatg gtatgtaacc
    11701 taaaactcct gtaagaagaa gcgcccctag aaattgagtc atcttatcac ctacttttta
    11761 ttttattata acacatttag tacctagtac taaattacgg gtagcccgac tacccttatt
    11821 attttttaat attttataga acatacgttc tttcaggagg tataaacatg tggattgaaa
    11881 aatttaaaaa caaaaataac gaaactaaat acagatacta cgagaagtat aaagatccat
    11941 acacagataa atggaagcgc gtaagtgttg tgttgaacaa gaatacaaaa caatctcaaa
    12001 aagaagcaat gtttcgttta gaagaaaaaa taaaagaaaa actgaacaac aagtcgtcaa
    12061 gcgaattaaa aactttgact tttcacgcgc tattagatga atggcttgaa tatcatataa
    12121 aaacatcagg ttcaaagttg actactctta ataatataaa aataagaatt agaaacatta
    12181 aacgatacag ctctgagaac ttgcttttaa acaaactaga tacaaaatat atgcagatat
    12241 ttattaataa attatcagat atctattctc aaaatcaagt aacccgtcaa ctcggagata
    12301 tgaaaggagc tattaaatat gcagttaaat tttacaatta tccaaatgaa tatttgttaa
    12361 ctaatgtcaa aattcctaaa agaagaaaaa caatagagga tatcgaaaaa gatgaatcta
    12421 aaatgtacaa ctatttagaa atgaaccaag tcctacagat acgtgatcat atactaaatg
    12481 ataataagtt acacaagcga aatcgcattt taattgccag catcttagaa gtacaggctt
    12541 taactggtat gcgcatagga gaactacaag cactgcagga aaaagatata gatttattaa
    12601 acaaaactat caatataaca ggtacaattc accgcattaa atacgaggaa ggattcggat
    12661 acaaagacac tacaaagact ataagttcaa aaagaagtat cagcatcaat tctagaaccg
    12721 tagaaatttt taaaaagata atactggaaa acaaaatgtt gaaaagatgg aattcgagct
    12781 atgttgacag agggttcata ttcacaacaa aaaaagggaa tcctttatgt aataatcaaa
    12841 tcgccggtgt gcttaagaaa actacaaaag ctttaaatat gaataagaaa gttaccacgc
    12901 acacatttag acatacacac ataactttat tagtagaaat gaatgtttct ttaaaagcaa
    12961 ttatgaaaag ggtaggacat gtagatgaaa aaacaaccat tcgcatatat actcatgtaa
    13021 ctgaaaaaat ggatagagaa ctaactcaaa aactcgaaaa cattccaagt tagcttaaat
    13081 ccgccctttt tttgccctta tattttttac aagctttata aaacgcttga gaacactggc
    13141 gttaaagctt ttcttgaaat aaacatatca tcataatgtg atggttcaaa aatcatttgt
    13201 actatcaaag gcttcatgtt cttaacaata tcatctaagt gattttctaa aatcggtgac
    13261 actgctttta aatcattaag aaaaggctcc catttgccta aagtattatc taattcttct
    13321 aatttagttt taatataatt acaagttaca ttaggaatca gggacaaaaa ttctttcttt
    13381 tttacattta acatttcaat tgcatgtctt aaattcttac gtattttggg aattgtatta
    13441 atcaaatatt ttattacatc gacaattttc gatgcatatt catcatatat accttgaaca
    13501 tagtctgcta tttttttaat accatcatcg atatggtctt ttaatatttt catttttctt
    13561 cctaaataat tagaaggtat aactagaccc tgtaccatat tttcgccgct acaattaatt
    13621 tggaaattcc catctaaaat tgttgcatct tgttgtttca taatacttct aatttctgca
    13681 atttgcctac cataaatatc attttgattt tttattcgct ctatattctg tttcactact
    13741 ttcaaatgtt tcatcatttc ttcagatact ccatctctga agtcgtgatc tatatttttg
    13801 aaaatttcta aaattt
//
LOCUS       DAHHRR010000067        13767 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.067, whole genome shotgun sequence.
ACCESSION   DAHHRR010000067 DAHHRR010000000
VERSION     DAHHRR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13767
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.067"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..572)
                     /locus_tag="NBI42_001682"
     CDS             complement(<1..572)
                     /locus_tag="NBI42_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCD1778099.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHNPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNL"
     gene            complement(726..1988)
                     /gene="clpX"
                     /locus_tag="NBI42_001683"
     CDS             complement(726..1988)
                     /gene="clpX"
                     /locus_tag="NBI42_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCD1778100.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAITELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDEEGNLINNSKTSA"
     gene            complement(2139..3440)
                     /locus_tag="NBI42_001684"
     CDS             complement(2139..3440)
                     /locus_tag="NBI42_001684"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCD1778101.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDESQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(3488..3598)
                     /locus_tag="NBI42_001685"
     CDS             complement(3488..3598)
                     /locus_tag="NBI42_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778102.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(3603..4532)
                     /locus_tag="NBI42_001686"
     CDS             complement(3603..4532)
                     /locus_tag="NBI42_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778103.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQLLTDFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(4551..5159)
                     /locus_tag="NBI42_001687"
     CDS             complement(4551..5159)
                     /locus_tag="NBI42_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCD1778104.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNIFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(5301..5657)
                     /gene="rplT"
                     /locus_tag="NBI42_001688"
     CDS             complement(5301..5657)
                     /gene="rplT"
                     /locus_tag="NBI42_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCD1778105.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(5704..5904)
                     /gene="rpmI"
                     /locus_tag="NBI42_001689"
     CDS             complement(5704..5904)
                     /gene="rpmI"
                     /locus_tag="NBI42_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCD1778106.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(5933..6460)
                     /gene="infC"
                     /locus_tag="NBI42_001690"
     CDS             complement(5933..6460)
                     /gene="infC"
                     /locus_tag="NBI42_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCD1778107.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(6489..6614)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(6689..8182)
                     /locus_tag="NBI42_001691"
     CDS             complement(6689..8182)
                     /locus_tag="NBI42_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1778108.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVITVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFETFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(8609..10546)
                     /gene="thrS"
                     /locus_tag="NBI42_001692"
     CDS             complement(8609..10546)
                     /gene="thrS"
                     /locus_tag="NBI42_001692"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCD1778109.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(10744..10866)
                     /locus_tag="NBI42_001693"
     CDS             complement(10744..10866)
                     /locus_tag="NBI42_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778110.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPAQDRYLKQRYLIHNLY"
     gene            complement(10959..11879)
                     /gene="dnaI"
                     /locus_tag="NBI42_001694"
     CDS             complement(10959..11879)
                     /gene="dnaI"
                     /locus_tag="NBI42_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCD1778111.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(11879..13279)
                     /locus_tag="NBI42_001695"
     CDS             complement(11879..13279)
                     /locus_tag="NBI42_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HCD1778112.1"
                     /translation="MGRQAFEFGLRPKDQFKVIQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVVLNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSTAKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(13280..13750)
                     /gene="nrdR"
                     /locus_tag="NBI42_001696"
     CDS             complement(13280..13750)
                     /gene="nrdR"
                     /locus_tag="NBI42_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCD1778113.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
BASE COUNT         4115 a         2668 c         1889 g         5095 t
ORIGIN      
        1 attaaattcc atatttgttg ttgtttatta ttttgaattg atgaataact tacaattgta
       61 tcgtctgggt ccatatctaa ttgcgtctta atatttttaa tatgcttttg aaccttacct
      121 tttggaattt tgtcttcttt agtgcatata actaaagtag gaatatcaaa atgtttcaaa
      181 taattgtaca ttaagatatc atcttgtgtt ggattatgtc ttaaatcaac taattgaata
      241 actaattgca aattctctct cttagttata tattcctcaa tcattttccc aaatttttca
      301 cgttgtgttt tacttacttt agcatatcca taccctggaa catccacaaa aataagttgt
      361 tcatctatat tataaaaatt taacgtttgc gttttgccgg gttgctgtga tgtacgtgcc
      421 atattttttc tgccaatcat actattgata aatgtagact tacctacatt agatcgaccg
      481 ctcagtgcaa cttcagacaa ttctgtttct ggatattgtt cttcctttac tgcactaatg
      541 attaattcta tattattagg attaactttc attttatatc ctcacttttt tatattctct
      601 ttcaactcta ccattatata atatttgaac ctttcggtat acattataaa aaagctccga
      661 tcaaagttaa actatttgag cgtcaacttt gattggagct tttcactttt ataacacatc
      721 aatgattaag ctgatgtttt actattatta attaaattac cttcttcgtc gtatagttct
      781 ggttcagttt cttcattaat tgtttgtgct gtaataacta ccttcgttac attttcgtta
      841 gaaggcacat caaacataat atcaattaac gattcttcta tgattgaacg taaaccacgc
      901 gcacctgttt ttctttcaat tgctttttca ctaattgctg ataaagcttc ttcagtgaac
      961 tctaaatcca catcatctaa ttccagcatt ttagtatatt gtttcacaag tgcattttta
     1021 ggttgcgtta agatgttttt caacgcagtt acatctaatg tttctaaatt agctacaatt
     1081 ggcacacgtc cgataaattc aggaatcaaa ccataggctt gcaaatcttc tgggcgaatt
     1141 tgtgctaata atgcttgttc gtcatattta tcagcttcat tgcttgagaa accaataact
     1201 ttttcaccaa gacggcgctt aatcacttct tcaataccat caaaggcacc accaagaata
     1261 aataagatat ttgttgtatc aatttgaatc atttcttggt ttggatgttt gcgtccacct
     1321 tgtggcggaa cactcgcagt tgtaccttct aagattttaa gcaatgcttg ttgaacacct
     1381 tcacctgaaa cgtcacgtgt tatagatgtg ttttcagatt tacgtgcaat tttatcaatt
     1441 tcatctacat aaataatacc tttttcagct ttatcaatgt caaagtcagc tgcttgaatt
     1501 aatctcaaca agatattttc aacatcatcg cctacataac cagcttcagt taagcttgtc
     1561 gcatctgcaa ttgcaaatgg aacattcaac gtcttggcta atgtttgagc taataatgtt
     1621 ttaccactac ctgttggccc aattaaggca atgttacttt tttgtaattc aacatcatct
     1681 tcttttggtc ctaattgttg aatacgctta tagtggttat aaacagccac agctaaagat
     1741 tttttagctt tttcttgacc aataacatat tcgtttaaat gatccataat ttctttagga
     1801 gtaggtaatt ctgtaatcgc ttcagaagtg ttttgagcta attcttcttc gacgatttct
     1861 gagcataatt caatacactc attacaaata tatacaccac ttcctgctac aagttttttt
     1921 acttgatctt ggtcttttcc gcagaaagag catttcaaat tttcttcatc ttcattgaat
     1981 ttaaacattc tttttacacc cctattcgtt aaagactata ctagattgga tattacaatg
     2041 caacatatta acatacaaac tttttgctta aagaatagta gcagatacat aagctatgca
     2101 tctgctactc atttttgtaa tttaatattt aatgaagatt aatcttcttt agttccttca
     2161 acgaactttg cgttatctct taataaatcg ataacttttt ggatacgaac atcattttta
     2221 atgatatcag tattacctaa agtatttttg atatcttcaa ctgagatatt aaattgttta
     2281 ctcatttttt ctaattcttt atcgatatct tcatcagtag cttcgatttt ttcagcttca
     2341 gcgatcgcag ttaaagttaa gttagtttta acacgttgtt ctgcatcgtc tttcatttgc
     2401 tctcttaatt gagattcatc ttgacctgag atttggaagt acgtttgtaa atctaaacct
     2461 tgttgttgaa ttctttgtgc aaattcagac accatacgat ctaattcagt attaaccatt
     2521 gcttcaggaa tatcgattgt tgtattatca gtagctttag taatcgcttc ttctttttca
     2581 acattttctg catctgtagc tttttgttca gctaaacgtt tacgtaagtt ttctttgtac
     2641 tcgtctactg tatttgcttc tgcatctaat tcattagcaa tttcatctgt taattctgga
     2701 acttccttga atttaatttc gttaactttt gttttgaaag ttgcttcttt accagctaat
     2761 tcttcagcat ggtattcttc tgggaatgtt acgacaacat ctttttcttc gtcaactttc
     2821 ataccttcta attgctcttc gaaaccagga atgaatgaac ctgaaccgat ttctaaatcg
     2881 taaccttcag cttgtccacc ttcgaattct tctccgtcaa ctgaaccact aaagtcgatg
     2941 ttaactgtgt cgccattttc aacaacacca tcttctttaa ctaccatttc agctaaatgt
     3001 cctaagctgt ggtcaattgc ttcttgtaac tcatcatcag ataattcagt ttcttgtttt
     3061 tcaatttcaa ggcctttata gtctcctaat ttaacttctg gctcaactgt aactgttgct
     3121 tcaaaaatga aatctttacc tttttcaatt tgagtaacac ttacttctgg ttgtgcaact
     3181 ggtttaatat cagtttcgtc aattgcttca ccataagcat ctggtaataa aatgtcgata
     3241 gcatcttgat ataatgcttc tacaccaaag cgttgttcga aaattggacg tggcacttta
     3301 cctttacgga atccaggtac gttaatttgt ttaaccactt ttttgaatgc ttgatctaac
     3361 gctttgttta ctttttctgc aggaacagta acagttaata aaccttcgtt accttccttt
     3421 ttttcccaag ttgctgtcat gtatatatac ctccatgatt aactaattta ttttttcaac
     3481 ttccctatta tatcataggt ctattcccta tacaaacatt gaaatcacaa cgtttatata
     3541 tttgtaaatc aacttttttc gtcaaaacta tattaaaatg ctgagtatta ttttgcaaca
     3601 atttaactat tattattcaa atctaattgc tgaataaaat taaatgcatc gatactttgt
     3661 aattcacatt taagtcctaa catatttttg aaataacatt cgtatgcatt aatccattta
     3721 ttcgtttcaa acaaagtttc aatatcaatt ggataaatca tgatagagtg attattcata
     3781 atatggtgtg cttcttcagc gatatgtaaa gcaccatcat ttaatgtttc cataacgtta
     3841 ggtataactt tttcttttag tgttgtatgt tctagccctt ttaaattagc tggtacaaaa
     3901 gttacatcca taccatactt ttcaattgta agttcttgtg tacaatttgc gaaccttaaa
     3961 tactcaatca ttaaactaat taggttataa ctgtacgtat tagattttaa tatatataat
     4021 accgtttctt gaaattgaaa atgaccattg tctattaact tcaaaatcaa gtgtgtctgt
     4081 tccctcattg ataacgtatc aaaatcagtt aataactgag tcaatcgttt ttcatcttca
     4141 attaattttg acttagcaaa ttctttaaga ggatgtaatg ccattcttgt tttgtgatct
     4201 ttgacttcat caataatttg atgaattact tctaccgctt caaaatattg tcccaaacca
     4261 atcaaacttt tcacgtaata aatcatcaat gcatcatatt gttgtatgcc agttttcaat
     4321 aagacaattg tttcttctct taattcaaga aatgaccccg tctcatataa catacggcat
     4381 ttcatcattg ctatttcttc agtgagttca aattgacgtt catattgtac aatatattca
     4441 tacatcaggt catagttttg gtcttgttca gcacgtttta tatctttata taactttttc
     4501 gaagagttag gaaactgaat aatatctgac atatatgtca cctcgtttga ttataaatct
     4561 tgaaggatta atgcgctcca cgtttcgaaa tcatcatatg actctatgtt gcctttttca
     4621 acccatttta ttcgtaaagt atctgtttct ttagcttcta catcattcgt acttacagtg
     4681 attttaaata caacaccaat atgtaccttg cccacttcat tattatcgtc attaataaaa
     4741 ccgatatatt ccatattttg tgaatcttgc tcacttaaac ctacttcttc ttccaattct
     4801 ctctgtgcat taactctcaa tacttcgtta atagattctg ctcctggaac atcattcata
     4861 tgaccgccta cacctattga agattgtcca tgcaatcgag cttctccacc gccagataat
     4921 cgttcataca ctaatatctc gccatgctca ttttcaagta aacaataaga aatgagttgt
     4981 ttatatgatg gatcctcttc catatcgccg cgtcgcttaa cttcatattg actgagcgca
     5041 tcaaaaatat tttgaccttc tggtttattc ttatttaaaa acccattgaa aatattcttt
     5101 tcattattaa aaataatttc tctaggtact acaatgattt gttcatcaaa tttagacatc
     5161 gtataaaact cctcacttta atgtttgttc tcttaaatgc attttagcaa atatgtcata
     5221 ttttagcgat taaatgtggc attttgctat ttaaaaaata aaaaagagat gacaatgcca
     5281 tctctttttt ataagtgtta ttattttaaa gcatctttag ctttagttac taattgagca
     5341 aatgcttttt cgtcagaaat tgcaatttct gataacattt tacggttaat gtcgatacca
     5401 gcttttttca aaccgttcat taaacgtgag tagctcattt catgttgacg agctgctgcg
     5461 ttgatacgtg taatccataa tttacggaag tcacgtttac gttgacgacg gtcacggaaa
     5521 gcatattgac ctgatttcat tacttgttgc ttagctactt tgtataatgt atgttttgaa
     5581 ccgaagtaac ctttagctaa tttaatcgtt tttttacgac gcgctcttgt tactgttcca
     5641 cctttaactc gtggcataaa aaattcctcc ttaggtaatt cctaccgatt tctgtatttg
     5701 ttcttatttt ttgtatgcta ataattgttt tacacgtttc atatcgctct tagacactaa
     5761 tctagcttta cgtaattgac gtttttgttt agtgctcttg tttgcgaata agtgagatgt
     5821 gaaagctctt gaacgtttta attgacctga agcagttctt ttaacacgtt tagctgctcc
     5881 gcggtgagtt ttcatttttg gcataatata agtcctcctg taatagcttt aattattttt
     5941 cagctgttgg cgctaacata ataaacattt gacgcccgtc cattttaggt ttttgttcaa
     6001 ctgttgctat atctttgcat tcatctgcat atttttctag cacacgttga ccaatttcct
     6061 tatgcgtaat ggcacgccct ctgaaacgaa tagatacttt acatttatcg cctttagtta
     6121 agaatttacg tccgtttttc aacttagttt ggaaatcatg ttcctcaatt gttggactta
     6181 aacgaatttc tttaacattg ataatttttt gtttcttttt catttctttt tcttttttct
     6241 gttgttcgaa tttgaattta ccgtaatcca taattcttgc aactggtggt ttcgcattcg
     6301 gtgcaacgac cactaagtct aaatctacac gttcagccat ttctaaagct tcacgctttg
     6361 atttaacacc aatttgttca ccatcttgac cgattaaacg taattctttt gcacgaattt
     6421 tgtcattgat ttgagtttga tcctttgcta tggttgacac ctccaaaatt tttacgaaat
     6481 ttgcaccaag caaaaaggaa gagcaggtat aaaatacccg ctcttcctta tacacagtta
     6541 tgtgtaatgt gattaacctg ccaactgctt tatgcgtcgc tacaggtgag aagcgggtgc
     6601 ttctacttgg ttcgtttcgt attcaacgtt gttaatcata ccaacaattc acatttaagt
     6661 caacactata actgtaatta tttttatttt aaccttttat ttcatccatt gacacgtctt
     6721 gtcgtaaatc tacttgttct aatggaattt ttttcgtttt atatcgaagc ttatgataaa
     6781 taaagaatgc taaaaatact ggaattccca tatacgtaat taaaaagcgg ctaaaattaa
     6841 aatctcctgt tttaataaag tccacatctt gaccgatgat tacaacaata cataaaaagc
     6901 cagcaaataa tggtccgaat gggaataatt tagcagtata ttttaattta gatttgtcat
     6961 aattttgttt atcaaatgct cttctaaatc gataatgact cactgcgata cctacccaag
     7021 caataaaacc agttaaacca cttgctgcaa cgatatattc gtatgcacct tttgtaagac
     7081 tttgtaatac gaaaataatc actacaatga ttgctgtaac gagtaaagac atatatggca
     7141 caccattttt atttgttttc ccaaatgttt caaacgccaa tttatcttta ctcattgaat
     7201 aaagcatacg cgttgaggca tacattccag agttacctgc tgataaaact gatgttaaga
     7261 taacagcatt cataaatgag gctgcaaagg caaatccagc atttttaaac actaatgtga
     7321 atggagacgt tgctacatta tcactaccac ccattaatgc actactatca taaggaatta
     7381 acataccgat aacaaaaatg gctaaaatgt aaaataataa aattctccag aatacttgtt
     7441 taatcgcttt cggcacagca cgttcaggat tttctgattc accagccgta ataccaatta
     7501 actcagtacc ttggaatgag aaaccagcga ttaagaatac acctagaatt gataacaaac
     7561 ttcctcctag gttgccacct aaaataggtc cttcaccttt attaaatatt tcaaatccta
     7621 caacatgtcc acccataatt cctacaatcg ttaataaacc aatagcaata aaaacaataa
     7681 ctgtaatcac ttttatcaat gccaaccagt attcactttc accatagacg cgaactgata
     7741 acgaattcag actaaaaatt ataactaaga acaacgcgct ccatgcccaa gcgggtatgc
     7801 cttgcaatgg tgtccaatat tgaatgactt gtgctgcaat cgtaatatct gctgctacag
     7861 ttactaccca gttaaaccaa tagttccaac caagcgcaaa ccctaaagat ggatcaacaa
     7921 atcttgtagc atatgtacta aatgaacctg atactggcaa ataagtagcc atttcgccaa
     7981 gtgacgtcat taagaaaaat accattattc cgataattgc gtatcctatt aatgcaccca
     8041 aagcacctgc atcatgaatt gctccaccag aagttacaaa taaacctgta ccaatacaac
     8101 ccccaatcgc aatcatagaa atatgacgat ctttaagtcc ccttttgaca acattgttac
     8161 tttcattttg aacttttgac atataagctc cctttccatg ttttagaggc caagtaataa
     8221 ataaccttta tttactttca tacccttttt ttacagaata tagtgatatt aaaagtaaaa
     8281 aagtggctac atacctttaa gatatgcaac cactttggca attatctata taaggtagca
     8341 catcacattc tgtgacagta cagttcctat tcgaaaactg tcccaataga tatagtttat
     8401 ggtttatatc tatttcggca tctttacctt tcacttgttc aacttatgta ccataaatac
     8461 ttctgacaag ttactaatta aacatgcaac ctctaactca atttaatatt ttaactaact
     8521 tgtaatatac aggattcatc acgcataatc aatcctgtaa aacttgatac gcaataaaag
     8581 tttcaaagca ttttattgcg acaactgtct atctatgttt tttcaaacga atttcatcaa
     8641 ctagattcca gataaattca tctttttcaa ctgtttcttg gtcttgcgat ccatattgac
     8701 gcacattcac ttgattattt tcaacttcct tatccccaac tacgatttga taaggtattt
     8761 tttgcatttg agcttctcta attttataac ccattttttc attacggtca tcaatactta
     8821 cacgaacgcc ttgagatttc aattcatctt gtaattggcg cgcataatca taatgtaaat
     8881 caacgttaac tggaatgatt tgaacttgtt ttggcgctaa ccaagttggg aatgcacctt
     8941 tagtttcttc agttaaaaat gctacaaatc gttccattgt tgatacaaca ccacgatgaa
     9001 taacaactgg acgatgatgt tcaccatctt gaccaatata agttaaatca aaacgttctg
     9061 gtaataagaa atcaagttgt gctgttgata atgtctcttc tttacccatc gctgttttaa
     9121 cttgaacatc tagtttcgga ccatagaatg ccgcttcacc aatcgcttct tcgtacgata
     9181 agccaagctc atccgctgcc tctttaagca tattttcagc tttattccac atgtcatcat
     9241 catcaaagta tttttcttta tcttcaggat ctctataact taatctaaag ctataatcct
     9301 cgaaaccaaa gtctttatac acatcaataa tcatgtttac aacgcgtttg aattcttctt
     9361 taatttgatc aggtcgaaca aagatatgtg aatcatttaa agtcatacca cgaacacgtt
     9421 gtaatcctga tacagcacca cttgcttcat atctatgcat agttcctagc tcagcgatac
     9481 ggataggtaa ttcacgatat gagtgtggtt tattcgcata aatcatcata tgatgtggac
     9541 agttcattgg acgtaatacc atagattcag tttcatctaa ctgcattggt gggaacatat
     9601 cttcttgata gtgatcccag tgaccagatg ttttgtataa atcaacatta gcaagtactg
     9661 gtgtataaac gtggtcatac cccatgctaa cttctttatc aacaatgtaa cgttcaattt
     9721 ctcgtctaat tgttgcaccg ttaggtaacc ataatggcaa accagcacca actaattggc
     9781 tatttgtgaa taattctaac tctttaccaa ttttacgatg atcacgttct ttacgctctt
     9841 ctaacatttg taaatgtgct tttaattctt ttttatcaaa gaaagcagta ccgtatatac
     9901 gttgtaacat tttgttgtta ctatctccac gccagtatgc acctgctgta gataataatt
     9961 taaactcttt aattttagct gttgatggaa cgtgaactcc acgacataaa tcagtaaaat
    10021 caccttgact atataatgtt acattttcat cttcaggaat cgcgtcgatt aattctaatt
    10081 tgtattcatc attgctgaat aactctttcg cttcatctcg tgaaaccact tttcgttcga
    10141 ttttcatatt ttcgttaacg atttgtttca ttgttttttc aatttgttca aagtcatcag
    10201 atgagatgtt ttggtcaatg tcgaagtcat agtagaagcc accttctatt acaggaccaa
    10261 caccaaattt aacattacca tataatcttt taatcgcatg tgccattaaa tgtgcagtag
    10321 aatgacgtaa tacctctaac gcttcttcac tacctggtgt cacaatttca attgatccat
    10381 cagtttcaag cggtttagtt aaatctacaa gttgcccgtt aaatttgccg gcaactgctt
    10441 ttttacgtaa tccaggacta attgattgtg ctatatcttc agtagtagta cctttatcaa
    10501 acgccttttt attaccatct ggaaattgaa tattaatttg ttccatgtta aaccctccta
    10561 tttgctttat aaatactttt atctgacctc gttaaaatat tgaacaacaa tcatcgtttt
    10621 aacattaaac ctattaacaa taaacaggga atttatgaca ccaacgaaca tttttgtcta
    10681 aaaaaaaaca aaaatcccgc ccctatacaa gggacgagat cgtcgtggta ccaccctagt
    10741 tatttaatac aagttatgta ttaaatatct ctgcttaaga taacggtctt gagccgggta
    10801 ttcattacaa ataccataaa tgaagtagta atcatctaat ttattaacca tattctcatc
    10861 aaacaatggc tttctgtgta atattgatta gatcatcttg tcttcaatca tttaaacgat
    10921 ttagatttgt attcattata caccaatcat tttaaaattc aattgtttct gaaattttct
    10981 cctgataaaa agtatggtgt tgacaaagat ttgacacgtt caataatacg tgctgcttta
    11041 gtcttctctt caccatcacg agtcatcgct aaatgatgtt ccaattcact atagtcaaaa
    11101 ttagaactaa agaatgttgg taattcatga accattcgat aatgtagcaa aggtccaatt
    11161 acctcatctc tcacccatgg agtcacttct tcagccccaa tatcatcaag cattaaaatg
    11221 tttgcttctc ttacgcgatg taatttcttt tcaaaagaac catctttaaa gccacctttt
    11281 aatgttctaa taaattccgg taaataaata attgtcgaac gtaccttctt agatttgagc
    11341 tgattcgcaa ttgcacctag aataaaagat ttacctgtcc caaatggacc ataaaggtaa
    11401 agacctttca cttgttcccc attagttatt gctgtacaaa tatcatctgc tgccatagca
    11461 acatcaagac ggtctcgatg attcatataa atatctttca atttggcatt taaagtatct
    11521 cgttgcatat gatgagatgt aattaactca gcttcaaagc gttcttcgtc gtacttgatt
    11581 ttacatgggc attgtaaata gcgtatttta attcggttat tatcaacata taactcaggc
    11641 acatgcccct ttacgaaatt tggacaatca gcaaatttat gaccgtcata atgtttttgt
    11701 tgatctttat actcttgtaa cacatttaag tcttcatcaa tcatagcatt tgttaattca
    11761 gctcgatgcg cttccaaaaa ttgcttaaca tctgggtcat tgattacttc tttttttatc
    11821 ttttctattc ttttttcaaa gtcctgcgac gtgttaatta tacttttaaa ttgcttcatt
    11881 attgactgtc ctcctcccat tttttagata atttatctag aaatgcttgt cgatcttgct
    11941 ctaattgttg atcatctacg ctattatctt tagccgaatc ttcttcacta ggtttatctc
    12001 tattttctaa ccatttaggt gttttttctt ttgaaatgcg attacgctgt ccatagtatg
    12061 aaccacgctt ttggtaattt ccgctagaac cctcattttt aggttgatta acttttttag
    12121 cgtaattata tgcttcttta gctgtcttaa tacctttttt cttccaattt gatgctattt
    12181 ccaaaatata cgctttagga agtttcatat cttcttttaa catgacaaat tgcaacaaaa
    12241 tattaatgac gccaaaagac attttttcac gttctattaa ttcttcaacc attgtctttt
    12301 gcgatatagt tggttctgat tcagaccaag aagctaacat atcaattgga ctcgtttgtt
    12361 caagtaattc aaaccattca tcactttgtg gctttggatt cacttctgaa gatttggccg
    12421 tcgaagatga tgtagcagga gatttcacct gtaatttagg catttgattt tcgtgttcca
    12481 ttaagtaata cgagcgtgct tgtttacgca tttcttcaaa ggataactgt tgcccacttg
    12541 taattgaatt taaaacaaca tgcttcatgc catctgctgt taaaccatat aaagtcgcga
    12601 gttgtgtaat taaacgcttt gcatctttgg taacaatgtc ttgactaata aaatgtttac
    12661 ctaacatttg tctcaacatt tcaaagtcaa aagattcatt tgataaatcg ataccttggt
    12721 acggttcatt aatcggaata tcacttgtat cgatatctat ttttgtagac ggcactttaa
    12781 aaacatcagt aaattgtctc gttacctgtt taaattcact caaatcaatt tgttgatact
    12841 caaagtattt cttcaactca tgaaatcgac gatgctcaac ttcactatat aaaaagattg
    12901 acaacattgg atcattaaaa aataaatgtg ctgaaggcgg ttgaattaat tggtaaacaa
    12961 attgtgtttc ttgttcatca tgtttgacaa acgcctttaa caatccaatc gcttcaagta
    13021 agtccatttg ttgtctaaac tctagtaaat taattttaag ttcattcata aaaatataat
    13081 gagaaagaat caatgtttca ttatgacttt ctttaacgaa ttgagtcata aaatgatata
    13141 aacccactgc ttgtgttcca attagcggtg tatacagtcg attcaatacc tctaaatgat
    13201 tcgtatttaa atcaaagtgt tgaataactt tgaattgatc ctttggtctt aagccgaatt
    13261 cgaaggcttg tcgtcccatt taagcatcac tccgtttgtt ttcgcttaaa atcccttgca
    13321 tcgacgctaa caattgatca acatctttaa attctttata gactgatgca aatctaacat
    13381 atgaaacttg atcaacatgc attaacaagt tcataacgtg ttcgcctata tctcgtgaag
    13441 acacttccgt atgaccttca tctcgtaatt gccattcaac cttgtttgtt atgtcttcaa
    13501 gttgttgata tctaactggt cgtttctcac aagaacgaac aagtccatta agtatctttt
    13561 ctcttgaaaa ctgctctctt gtgccatctt ttttcacaac tataagctga ctaacttcga
    13621 tatgttcaaa tgtagtgaaa cgtgttccac aattttcaca ttctcttcgt cttcgaatgg
    13681 catttaattc atcggcatgc cttgaatcta caactttaga ttgtgtagaa ttacatttcg
    13741 ggcatttcat tacatcaccc tctttat
//
LOCUS       DAHHRR010000068        13696 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.068, whole genome shotgun sequence.
ACCESSION   DAHHRR010000068 DAHHRR010000000
VERSION     DAHHRR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13696
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.068"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(42..677)
                     /locus_tag="NBI42_001697"
     CDS             complement(42..677)
                     /locus_tag="NBI42_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1778114.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFNNNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEITNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            998..1798
                     /locus_tag="NBI42_001698"
     CDS             998..1798
                     /locus_tag="NBI42_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1778115.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKPRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYKGNELYNYKSFNRQVFQQVVDYLNMNQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWGMSSSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLNK"
     gene            complement(2043..3035)
                     /locus_tag="NBI42_001699"
     CDS             complement(2043..3035)
                     /locus_tag="NBI42_001699"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCD1778116.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            3305..3976
                     /locus_tag="NBI42_001700"
     CDS             3305..3976
                     /locus_tag="NBI42_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD1778117.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAETIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEW
                     VGPRD"
     gene            complement(4097..4903)
                     /locus_tag="NBI42_001701"
     CDS             complement(4097..4903)
                     /locus_tag="NBI42_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1778118.1"
                     /translation="MAFHDKTATQVTNIELNVRDLELMTTFYKNILGLSVKSSDDNKS
                     VLSVGNGGHTLTLHLLEDGRQPSSREAGLFHIAFLLPTTEDLANFLYFVAQKHMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRSSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKCMIGHLHLKTHDLNAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNAQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(5077..5361)
                     /locus_tag="NBI42_001702"
     CDS             complement(5077..5361)
                     /locus_tag="NBI42_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1778119.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLVKAVVEHARENNLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            5589..6023
                     /locus_tag="NBI42_001703"
     CDS             5589..6023
                     /locus_tag="NBI42_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1778120.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMASLTEIGQS
                     QMADIFPKHAETLTKAFDVLTEDELATLQQAFKKLSAQSTEV"
     gene            6101..7060
                     /locus_tag="NBI42_001704"
     CDS             6101..7060
                     /locus_tag="NBI42_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCD1778121.1"
                     /translation="MTNNHKLLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPDIPKGQAGTNSITRPSFRVPNDEALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPEDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIEHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVENHDALKAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEIRPFNTKR"
     gene            7091..7684
                     /locus_tag="NBI42_001705"
     CDS             7091..7684
                     /locus_tag="NBI42_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1778122.1"
                     /translation="MEHIFREGQKGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLEFRGQELLAFIKEADERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSNVSVLLSMGKHDPIVPLAASE
                     QVINLFKTRGAVVEEVWVNGHEITESGLTAGQQILEK"
     gene            complement(7789..8868)
                     /locus_tag="NBI42_001706"
     CDS             complement(7789..8868)
                     /locus_tag="NBI42_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778123.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMIVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSKRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVIYKGLIKRITGKSHID"
     gene            complement(9217..11181)
                     /locus_tag="NBI42_001707"
     CDS             complement(9217..11181)
                     /locus_tag="NBI42_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCD1778124.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQSCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNADEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            complement(11562..12326)
                     /locus_tag="NBI42_001708"
     CDS             complement(11562..12326)
                     /locus_tag="NBI42_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1778125.1"
                     /translation="MGTLIKQECFKLYKKKSTFIAPIVFILLMVAQGYIATKYNITPK
                     ENFTSGFNGLAWFSFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIISKIITLFII
                     SLIYFAITIIASIGIGSLFFNDLNIFESSGNQLSLFNQLLLVSLGTFVGVWLVLSLTL
                     LLSCATNSTGVAIAVGIVFYFASTILAVVQTTLLEKIEWLKWNPINMMNIMIQTVEKG
                     FSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNV"
     gene            complement(12328..13023)
                     /locus_tag="NBI42_001709"
     CDS             complement(12328..13023)
                     /locus_tag="NBI42_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1778126.1"
                     /translation="MDVVTVEHLTKKIGNKTILEDISLTLKRGHIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVIKNKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLNMEEYIHKKAKTYSLGMKQKLGIAIAFLNDPQFVILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLDIIEREDQA"
     gene            13395..>13696
                     /locus_tag="NBI42_001710"
     CDS             13395..>13696
                     /locus_tag="NBI42_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1778127.1"
                     /translation="MKREVIFKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDKVNNKFLSEMLNVSKPAITKSIKKLLAKGLVVESHNEFNKREVNYSLTQEG"
BASE COUNT         4739 a         2081 c         2353 g         4523 t
ORIGIN      
        1 aatacttttt ttaaattcta atttcaaagt ggacacctca attataaatt aatgtaatca
       61 tttatgactt cttcttttga ttggtactct tctatttgaa ggtctttaaa aataaagtat
      121 ttacccggca aagcacttaa atcggataaa ttatgtgtaa tattgataat agttttagtt
      181 tgatggcttt gaataaaatc atttaaaaat tcataaattt cattaactgt tttcttgtct
      241 aaagcgtttg taatttcatc taatataatt aaatcatgat cttccaataa gaaacttaat
      301 aatttaagct tttgtttttg accatcactc aagtttttta gtagtacgtt agacggtatt
      361 gcttcgatgt ttagtatgtt ataaatctta tcgaaggttt gtcgattgtt attaaatttt
      421 gattttaaca atgataataa aaaatctttt gttatatcat taggaatatt tgatgaactt
      481 gatattaagg ttacattttg agaaatggac ttagggatat ttccactatt atttagtaag
      541 aaatctttag ctaataaaga ttttcctaca ccatttttac caacaatgtg attaatctga
      601 ccaagataga aatttaaatc acaattgtca actagttgtt tgtttttaac ttttaaagaa
      661 tagttattta gtttcatgta tacaactcct atgtataaag ggatttatta caccgatatt
      721 taattgtatt tttaaaaatt ctttcacatt atgtatagat gttataaatt agtatatcac
      781 actatatttt gttcaaatga taaatataac tttatatttt tacaatattc tgaaatttat
      841 gtgcgcttct gaatgctata tccagtgtaa tatgttttgc atatatgaaa gcaatttcaa
      901 aatgtgaata taggttcatt gtggtatgac aaacttcatt gcttgtcatg agatggatat
      961 aatgaagacg actaaaagag caatgaggag gttaatcatg gttaaggcta ttgcggtaga
     1021 tatggacggg acgtttttag attcgaaaaa gacatacgat aaacctagat ttgaagcgat
     1081 ttttactgaa cttagaaata gagatattac atttattgct gcgagtggca atcaatatgc
     1141 gaagttgaag tctattttcg gggatagaga tatgtatttt atatctgaaa atggtgcagt
     1201 tatttataaa ggcaatgagt tatataatta taaaagcttt aatcgtcagg tgtttcaaca
     1261 agttgtcgat tacttaaata tgaaccaaag tattgatcaa ctcgtcatct gtggtttgaa
     1321 aagcgcgtat attttgaaac atacttctga agcatttaaa gaagatacga gattttatta
     1381 tcatcagtta aaagaaattg acagtctaca acaattacct gaggatgatt atgtcaaaat
     1441 agcattcaat attaatcgtg agacgcatcc gaatgttgac gaagaagtag cgacgcaatt
     1501 cagcaatgat attaaacttg tctcaagtgg gcacgatagt attgatatta ttatgccaaa
     1561 catgactaaa ggacaggctt taaaacgatt gttagataaa tggggaatgt cttcttcaga
     1621 gctcatggcc ttcggagatg cgaataatga taaagatatg cttgcgttcg ctaagcatag
     1681 ttatgtgatg gaaaatagtc atgatgaaga attgttcaat attgcgtcgg ctgtcgctcc
     1741 aagtaacgat aagcaaggtg ttttaacaat tatcgaacaa gaagtattga acaagtaggt
     1801 agtacttcat caatgaagta gctatcatga tactaaatac atgttaaatg aaaactttga
     1861 atacaaatca tcaaatatta atatatctca aacaggttag gcaataatgc catagtaaaa
     1921 taagcgtact acaaccattt gaatagatga taaaataatg cggattttaa tcaaactggt
     1981 tttgatacag atatacaaaa taaaaagcaa aggcacatgg cgcgcctttg cttttaaata
     2041 tattaattta aacgtgtttc acatgtacca gtgttaatga cggataatgc tgcgtttaaa
     2101 ccaccttcaa caaggttttg tactgcttca tcagagaaga aagcaatatg tggtgttact
     2161 aaaattcttt catgttcgat taactctaat aatgttttat cgtcaatgtc tttattagtc
     2221 cagtcatttg tgaagtatgc tgcttcattt tcataagtat caatcgcagc acctaataaa
     2281 gtaccatcgt tcactgcagc gattaaatca ggtgtattga tgactgcacc acgtgctgcg
     2341 ttaactaaga ttgcaccttt tttcacatga tcaaacattg ctttatcgaa taaatgatag
     2401 ctttctttgt tcgctggaac atgtaaagaa ataatatcag catctttaat agcttcttta
     2461 acactatctt tgtatgttaa aaagtctaaa tctttattag gataagcgtc ataagctgta
     2521 attgtagcac caaatcctgc atatatttta gctgtagcag caccgatacg accagtaccg
     2581 ataattgcaa cagtcatatt tttaactggt ttagacatga tttctgcttg ccaagtaaaa
     2641 tcatgtgctt gtacacggcg ttcaatatct gggaagcgac gcactaattg tagggcgata
     2701 gaaacagaat actctgcaat tgtttcaggt gaataacttg gaacgttaga tatcacaata
     2761 ttgtgttttt tagctaaatc taaatcatac atatcaaatc cagcagtacg ttgtgcaatt
     2821 tgtttaatac cgtaagattc taatttagga taaacgtcat tttctaattt accaaattgc
     2881 attgtagtta cgccatcgta atcttttaat tgatcgacag tagcacttga taatagctct
     2941 ttagaagtag ttacttcgac gttattcttt ttcccccaat ttaatgccat ctctttctca
     3001 taatcacgcg taccaaagaa cataattttt gtcattatta aaaacctcgc ttttaaaaga
     3061 ttgaaaagta aatgagtgaa attaaagatc atgcacatta aaatcacgcc acaatttaat
     3121 tgtgaaaaat atcacaaata tattataaca ctaaattttc caaaattcaa aaacgtgttt
     3181 aattgtagaa aacctataac atgcgcacaa gttataatag attctaaacg gattacttta
     3241 gactttttaa aacatgaagt ataatttgta tagcaataaa tataaaaatc ggaggctatg
     3301 ttcaatgagc aatatgaatc aaacaattat ggatgcattt catttcagac atgcgacaaa
     3361 gcaatttgat ccacaaaaga aagtttcgaa agaagatttt gaaacaatat tagagtcagg
     3421 tagattgtct ccaagttctc ttgggttaga accttggaag tttgtcgtga ttcaagatca
     3481 agcgttacgt gatgaattaa aagcgcatag ttggggcgca gcgaaacaat tagatacagc
     3541 gagtcacttt gtgttaattt ttgcgcgaaa aaatgtaacg tcaagatcac cgtatgtaca
     3601 acatatgtta cgagatatta aaaaatatga agcagaaacg attccagctg ttgaacaaaa
     3661 attcgatgct ttccaagcgg acttccatat ttctgataat gatcaagcgt tgtatgactg
     3721 gtcaagtaaa caaacgtata tcgcattagg caatatgatg acgacagccg cattgttagg
     3781 tattgattca tgtcctatgg aaggttttag tctggataca gtgacagata ttttagcaaa
     3841 taaaggcatc ttagatactg agcaatttgg tttatcagtg atggtcgcat ttggctacag
     3901 acaacaagat ccaccgaaaa ataaaacacg tcaagcatat gaagatgtta ttgaatgggt
     3961 tggacctaga gactaattag tatacagtat atttaaatat ataaatttta aaacatagca
     4021 ataataaaag cctgcgttca cataaatgaa tcgcaggctt ttgcgtgaaa aaattgtatt
     4081 aataaaatat ggatgactat ttttctggaa caaggtcagt atttgaatga actgtgatgt
     4141 caaacccttc tggtgccgta aatgtatgtg ttgaggcgtc gggttgataa atatcaacat
     4201 gtgttaatcc ataactttgt gcattgtttt gtcttgcttg attagattgc caagtgttag
     4261 cagcaatatg atggtgataa tgattcgttg acataaataa cgcacgtgga aaatcagaca
     4321 catgttggaa tcctaattgt tcaatgtaac attgatatgc tgcgttcaaa tcatgtgttt
     4381 ttaaatgtaa gtgtccaatc atgcattttg ctggcatacc ttgccaacct tcgtcagtac
     4441 gatgtgttaa taatgtttgg ctatcaactt ctaaagtatc cattttaact ttgccatttt
     4501 gccattccca tgaagatgaa gatctatcgc gatagacttc aataccatta ccttcggggt
     4561 cattgaaata taaagcttca ctgactaaat gatcaccagc accaatgccc atatgttttt
     4621 gtgccacaaa atataagaag ttagctaaat cctcagtagt tggtaataaa aatgcaatat
     4681 gaaatagccc tgcttcacgt gaggaaggct gacggccgtc ttctaataaa tgtaacgtta
     4741 gtgtgtggcc accattacca actgataata cacttttgtt atcgtcagaa cttttaacgg
     4801 atagtcctaa aatgtttttg taaaatgttg tcatcaattc taaatctctt acgttcagtt
     4861 caatgtttgt cacttgtgtt gctgttttat cgtgaaatgc cattatgcat cgcctcgttt
     4921 tctatttttc tataagttag tataaaaagt ataccagaaa aggaaatgaa ttgatagcat
     4981 aaagtttgaa atgcaaaata acttgtcgtt ttgcaatttt atattgattc gtacaaataa
     5041 aaagcgatgg tacagttgca ccatcgcaaa atttatttaa ccaagatata catcttgata
     5101 tgaatcttct ttttctaaca tatgtttggc aaatgaacat gaggcaataa ttttcaaatt
     5161 attttctcga gcgtgttcaa caactgcttt aactagtttt ttgccaacac cttgaccacc
     5221 gagttcatca gatacgcctg tatgatcaat gttaatttca ttattatcca caaaacggta
     5281 tgtgatttca gctaaagcat tattttcatc atcaccaata tagaatttgt tctcgccttg
     5341 tttgatttca aggttactca tacatatcaa ctcctatcat gattgattat aatatttccc
     5401 tattctattt taacttaaac gaaatcaaag gtgcatgaca gtcatgtgac gacattgcct
     5461 ctgtacaagc agttattttc attaagcgca gtttgaaata aagatgaaaa cacgtatctt
     5521 gacattaaat ctattcagct atataattta tctcgaaatc gaaataaaat gaaaaagttg
     5581 gtgatcatat ggatcgaacg aaacaatcgc tcaatgtctt tgtcggaatg aatagggcgt
     5641 tagacacatt agagcaaatt acaaaagaag acgtaaagcg atatggctta aatattactg
     5701 aatttgcagt gcttgagttg ctttataaca aaggtccaca accaattcaa cggattagag
     5761 accgcgtatt aattgcaagt agcagtattt catatgttgt aagtcaatta gaggacaaag
     5821 gttggattac acgtgaaaag gacaaagatg ataaacgtgt atacatggct agtttaactg
     5881 aaataggcca aagtcaaatg gcagatattt tccctaagca tgctgagaca ttaacaaagg
     5941 catttgatgt gttaacagaa gatgaactgg caacgttaca acaagcgttt aagaaactaa
     6001 gtgcacaatc tacagaagtg taaggcgtgc actaaaaatt tacattaaaa tatctcggtt
     6061 tcgagataaa tatactgaaa agacaaagag ggtatataaa atgacaaata atcataaatt
     6121 actaggtata caccatgtta ctgcaatgac agatgatgca gaacgtaatt ataaattttt
     6181 tacagaagta ctaggcatgc gtttagttaa aaagacagtc aatcaagatg atatttatac
     6241 gtatcatact ttttttgcag atgatgtagg ttcagcaggt acagatatga cattcttcga
     6301 tttcccagat attcctaaag ggcaggctgg tacaaattct attacaagac cgtcttttag
     6361 agtgcctaac gatgaagcat taacatatta tgaacagcgc tttgatgagt ttggtgttaa
     6421 acacgaaggt attcaagaac tatttggtaa aaaagtgttg ccatttgaag aagtagatgg
     6481 ccaagtgtat caattaattt cagatgagtt aaatgaaggt gtagcacctg gtgtgccatg
     6541 gaagaatgga ccggtgccag aagataaagc gatttacgga ttaggtccca ttgaaattaa
     6601 agtaagttat tttgacgact ttaaaaatat tttagaaact gtttacggta tgacaactat
     6661 tgagcatgaa gataatgtcg cattacttga agttggtgaa ggcggcaatg gtggccaagt
     6721 aatcttaatc aaagatgaca aagggccagc agctcgtcaa ggttatggtg aggtacatca
     6781 tgtgtcattt cgtgtggaaa atcatgatgc attaaaagcg tgggcaacga aatataaaga
     6841 ggtaggtatt aacaactcag gcatcgttaa tcgtttctat tttgaagcat tatatgcacg
     6901 tgttggtcat attttaatag aaatttcaac agatggacca ggatttatgg aagatgaacc
     6961 gtatgaaaca ttaggtgaag gtttatcatt gccaccattt ttagaaaata aaagagaata
     7021 tatagaatca gaaataagac catttaatac gaagcgttaa catgattaat tggaatgagg
     7081 aggatttgtg atggaacata tttttcgtga aggacaaaaa ggtgcgccaa cgctaatatt
     7141 gttgcatggt acaggtggcg atgagttcga tttattgcca ttaggcgaag cattgaatga
     7201 aaattatcac ttgttaagta ttagaggaca agtttcagaa aatgggatga accggtattt
     7261 caaacgtctt ggtgaaggtg tttatgatga agaagatttg gaatttcgtg gacaagaatt
     7321 gttagcgttc attaaagaag ctgatgaacg ttatgatttt gatattgaaa aagctgtgct
     7381 tgttggattt tcaaatggat caaatatagc gattaactta atgttgcgtt cggaagcacc
     7441 atttaaaaaa gcattgttat atgcaccgtt atacccagtt gaagtaacgt caacaaagga
     7501 tttatcaaat gtaagtgtgt tgctttctat ggggaaacat gatccaattg tgccattagc
     7561 tgcaagtgaa caagtcatta acttgtttaa gacacgtggg gcagtagttg aagaagtttg
     7621 ggtgaatggt catgaaatta cagaatctgg attaacggct ggtcaacaaa tacttgagaa
     7681 ataacgatac tataataaag cggacagatg gaaaagattt ttacttttca tctgtccgta
     7741 tttttgattt tgaagggctg tactaaattt taaaatggta tagatatttt aatcgatatg
     7801 agatttgccc gtaatacgct taattaaccc tttatagatt acaggtatga gtaagatgaa
     7861 accgaacaat cccatgatag ggaatacttt tccaattaat gaaatgaaac cgataaatgt
     7921 actaatataa gtgatgacag ccattgtaat aataatgatg aagtaacgtt tgctaaatgg
     7981 aacgctgaaa cgtgacgcaa atgcatacat taatccaaca acagtattgt agataacaag
     8041 tatcataatg acagacataa taataccaat tgacggagac atttgtgtcg ctaattttaa
     8101 tgtaggtaaa tcaacgtgtt taattttatc gaattgagaa attaaaccta gattaatcat
     8161 catgagtaaa aaagtaatga ttaaaccgcc aatcaagccc ccgtataagg ttgagtcacg
     8221 atatttaact ttgctaccca tcactgataa gaagctgaag gcagcagcaa tttgcaagct
     8281 tgcataatta atagcatcaa accaccaacc aggtgataat gatttctgct tatgaatctg
     8341 agcatcatta ttagcggcag taaaatcaag atgactcgtt gtgaaatagt acaccgcaat
     8401 cataattaca atcgcaatta aaaatggggt aacaccacca agcacagcaa ttaaacgatc
     8461 gaattttaga aacagtgttg ctaaaataaa ggcgactaag atgagtgcgc tcagccaata
     8521 cggtaagttg aaactttgat gaatggttga agcgccccct gcagtcataa taatagctag
     8581 agacaacata aacattgtca aaataatatc aaaacctctt gcaatagagg ggtataagaa
     8641 atagttaatt gaatcagaat gatttctaga ctttagatga tgacctgtat gcatgacaat
     8701 cattccacct aaagtaatca atagtcctgt cacaataatg cctgaaatgc tatatgcgcc
     8761 atgacttgtg aaaaactgga aaatttcttg accagtagca aagccggcac caacgacaac
     8821 accaacaaag gcaaatgcca caataatgga ctcttttaag atacgcatga tttaaaaatg
     8881 tcccttcgta attttaagta atatagaaaa tgtaacatac atgttaatga aaaatatagt
     8941 actaatatag tattttgtta aattggagta gaagcgaggg cgtcggttat tttattagtt
     9001 tattagttga ttttccattt ttttgctgta aagatattat aatacagtta acaaggtact
     9061 agcatagaca caccaatccc ctcactattg cgagcagtga ggggattttt ttcggtgtag
     9121 ctaggtcgcc tatttattta ccatcgcgtt tgcgtagcca atgcgcaaat actgcaacaa
     9181 tacaacagta cacaatacat gcatcaaatg tcgtctttag tccaagtaac gatcatgcat
     9241 taacattttt aaaatatcta tttgagcttg aagatcttta ccaatattgg tatcacgaat
     9301 cttcttacgt tgtaattctt tatctacgac gcgctttata gaaagttcat cgataccttc
     9361 ggaaagtatt ttttctttag cgttaaattg ttggtgtgca acgagttgca taccgaatga
     9421 attatacaat agtgtatagc ctgcaatgcc agttgttgac tgataagctt ttgaaaagcc
     9481 accatcaatg acaagcatct ttccatcagc cttgatagga tcttcgccat tgatttcttt
     9541 cactggtgtg tgaccattaa taatacgacc ttcatctgca tttaatccga aatcactgag
     9601 cattttacga accatattca catcttcacg tagatgatag tatggatttt tttcttcttt
     9661 atgagaagct ttatctgcaa taaagtatcg ctcaaacgtt gtcatggcac gtttaccaaa
     9721 taatgacgaa tatttcccag tccataaata ccaaactaaa tccgtcgata agtcatcagt
     9781 attttctttt tcatcaaatg atttacggac atgatactca aacacatcta ataattcttg
     9841 gccactgtaa gtatgaccat caatttcaaa tgattccatc tcaccatttt catcaactgg
     9901 aatacaacca tgaatgagta aattgccatt atatggtaag taaagcgaac ctttacgcat
     9961 caagaaagac atatgacgac gtaatttttc agattgttgg aatgacaata atagtttatt
    10021 catgacttct tcttcttcag gtagtaattc tgctggatta tcacgattga cagtttggaa
    10081 acatgtgtct ttcaacgggt atgtattacc ataaactgta atttcatttg tatcataatt
    10141 aaccttttca agcacaagac gttcttccat ttcgaaattt ggacgacgtt taataattgg
    10201 tatttctaac ttgaattgaa tcatcgcaat agcttgatga attttagtaa tttgactttc
    10261 ttcacgttga gttaaacgtt catgtttgtc aggtcttttt ttaggtttaa aagcaggatt
    10321 atctgcgtca tagtattttt cagctaaagt aagcagtggt cttaaattaa tgccataagc
    10381 gtcttcgata atatctaaat tatcataacg tgcacaaatt cgaagtaagt ttgctaagca
    10441 tacttttgac ccagcatagg ctccaaccca aagcacatca tgattacccc attgaatatc
    10501 tagggaatga taattaatca gtgtatccat aattttatct ggttgtggtc cacgatcata
    10561 aatatcaccg acaacgtgta aatgatcgac gactaagcgt tgtacagaat acgcaagccc
    10621 tataattaaa tcatctgcct gtttaagttc aattacttgg ttaacaagtg tttcgtaata
    10681 tgatttttta ttttgatatt cattactttt atacagtagt tcctcaataa tataaacgta
    10741 ttgctttggc aatgctttac gcagtttaga acgcgtatac tttgaagaac aatatttaat
    10801 caactcaatt aaatgttcga ttgttgtgat ataccagaca ttaagttgac cgcaactttg
    10861 gaaatcactt ttaatcaatt ttaatttgtc ttctggatag tagacaagag cagttaagtc
    10921 attaagctcc ttagttgaaa gtctctcttt gaaaatatca ttgattttcg ctcgcacgtt
    10981 cccagaaccg ttgcgtaata cgtgttggaa agcttcatat tcaccatgta aatcgctgac
    11041 gaaatgttcc gtacctttag gtaattctaa aattgattct aaattgataa tttcagttgc
    11101 aagtttttct ggagtatcaa aattttgaga tagtaaatct aaatacttct tttttaattc
    11161 tttttcagtg atttgagtca ttacgtgtca ctcctgtagt atatttcaaa cgttagttta
    11221 taaaataaat gcgcttacaa tgattatcaa gaaaacgtta acatttttca ataagtatgc
    11281 ttaaattgat aaaaatagag gacaagattt tgagaatttt agtattagga gccgttgcag
    11341 tttagttaag taaaagtggg gaagtgatat atttatttta aaaatgagag gagggatttg
    11401 ttatattcac aagtttccta aaaaattacg atatgtaaaa tgtgaaattg ccaaaatttg
    11461 ggtgaaaaat ccccgcacca ttattttggt gaagggattt gaaaatgatt ttggaatacg
    11521 ctggaatatg tgtctgttta gacatctaat actttaagtt actatacatt tttcttcttg
    11581 aaaataaata ctacaaggat taagaaaata gagatataag caatattacc aataaacaat
    11641 tcgtgaagtt ctaatttcgt cgacttacta aaaccttttt caactgtttg aatcataata
    11701 ttcatcatat taatagggtt ccactttagc cattcaattt tttctaaaag tgtcgtttga
    11761 actactgcta atatagtact tgcaaaataa aaaacaatac ctactgcaat ggctacgccc
    11821 gttgaatttg ttgcgcatga taatagtaac gttaagctta aaactaacca aacgccaaca
    11881 aatgtgccta aactaactaa taataattga ttaaataaag ataattgatt cccgctactt
    11941 tcaaatatat ttaaatcatt aaagaataaa gacccaatac caattgaagc gataatggta
    12001 atagcaaaat aaattaaaga aataataaat aatgtgatga ttttgctaat aatcatagtt
    12061 gttcttgaat attcacgata aagtaaattt ttaatcgttc cgtaatgaaa ttccattgaa
    12121 ataattgtac ttgcttgaat aattaataaa aatgaaaacc atgcaagtcc attaaaacca
    12181 gatgtgaaat tttcctttgg cgtgatattg tatttcgtag caatataacc ttgagcaacc
    12241 attagtagaa taaagacaat aggtgcgata aaagttgatt tcttcttata taatttgaaa
    12301 cattcttgtt taattaaagt tcccatttta tgcttggtcc tccctttcta tgatgtctag
    12361 taatacattt tctaaatcat tatctttaaa ttgtttaagt tcttcttctg atgtttctgt
    12421 tacaatttta cctttgttga taataaggat agagtttgta attttaacca attcacttaa
    12481 aatatgactc gaaattaaga aagtaacacc ttcttgcgct ttttggacaa tcaattcacg
    12541 tacatctcgc acagcttttg gatctaagcc gttcattggt tcatctaaga taacaaattg
    12601 aggatcattt aaaaatgcta tagcaattcc taatttttgt ttcataccaa gagaatacgt
    12661 tttagctttt ttatgaatgt attcctccat attaagttgt gagacaattt tatcgatatc
    12721 ttgagtgttt tttgattcgt tcaataactt caagttttca tatccagaca taaaaggata
    12781 tattcctgga ttttcaatca atgcaccgat attgcttttg ctgtccttgt ttttaataac
    12841 gttgaaattc ccgctttgga aactagagta acctaatata actttcatta aagttgtttt
    12901 tcctgcacca ttcgctccaa caagacctac aatgtgtcca cgttttaacg ttaaggatat
    12961 atcttcaaga atcgttttat ttcctatctt ctttgttaaa tgttcgactg ttacaacatc
    13021 catatcacta tctcctttac aattaaataa tctgaatctt ctaattttgt atataaaatt
    13081 aattttatca caagtcaaga gttattaaaa tatagattta aaaaacttta ataatataaa
    13141 aagttaatac aaatacgtgt atttttgcag catgcaaatg gcattgctaa atttattaaa
    13201 atgcgcaatt tgttatgagt gacttttttg aatagaaggc atattaatga ataatgaaca
    13261 tagtatagtg gatttataat atgagaaata ataagtaaat aactgttaat tacaacagtg
    13321 tttgacaaaa gagagagaat aaagaataat aaagttaacc ggttaactaa tgaagtggag
    13381 gaataataat ggatatgaaa agagaagtta tttttaaatt acagcaattc attatagaaa
    13441 gagaaaatgc tgataaaaga agacagtata aaaaagaaaa tgaggaattg gatttatctc
    13501 taacacaatt tcatattata gagttgattg ataataatga taaagtgaat aataaatttt
    13561 tgtctgaaat gttaaatgta tcaaaaccag caattactaa atcgataaaa aaacttttag
    13621 cgaaaggctt agtagttgaa tcgcacaatg aatttaacaa aagagaagtt aattattcat
    13681 taacgcaaga aggaaa
//
LOCUS       DAHHRR010000069        13586 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.069, whole genome shotgun sequence.
ACCESSION   DAHHRR010000069 DAHHRR010000000
VERSION     DAHHRR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13586
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.069"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..1347)
                     /locus_tag="NBI42_001711"
     CDS             complement(<1..1347)
                     /locus_tag="NBI42_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1778128.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGIIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EEVEGHPFKMPGGAVSNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFI"
     gene            1627..1983
                     /locus_tag="NBI42_001712"
     CDS             1627..1983
                     /locus_tag="NBI42_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778129.1"
                     /translation="MLKQILPRATKISVIFAVAFFIINYIGMYKPDILYLVGRTIIAT
                     LAFILICLTLFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            1980..2165
                     /locus_tag="NBI42_001713"
     CDS             1980..2165
                     /locus_tag="NBI42_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778130.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(2261..2452)
                     /locus_tag="NBI42_001714"
     CDS             complement(2261..2452)
                     /locus_tag="NBI42_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778131.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(2463..3098)
                     /locus_tag="NBI42_001715"
     CDS             complement(2463..3098)
                     /locus_tag="NBI42_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1778132.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(3173..4090)
                     /locus_tag="NBI42_001716"
     CDS             complement(3173..4090)
                     /locus_tag="NBI42_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCD1778133.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANDWLEVSFLSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            4358..4888
                     /locus_tag="NBI42_001717"
     CDS             4358..4888
                     /locus_tag="NBI42_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778134.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(4954..6558)
                     /locus_tag="NBI42_001718"
     CDS             complement(4954..6558)
                     /locus_tag="NBI42_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1778135.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVVIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASTGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            6888..7652
                     /locus_tag="NBI42_001719"
     CDS             6888..7652
                     /locus_tag="NBI42_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1778136.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTEKMYTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(7710..8216)
                     /locus_tag="NBI42_001720"
     CDS             complement(7710..8216)
                     /locus_tag="NBI42_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCD1778137.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIKADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(8383..9696)
                     /locus_tag="NBI42_001721"
     CDS             complement(8383..9696)
                     /locus_tag="NBI42_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCD1778138.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     regulatory      complement(9773..9868)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9921..10490)
                     /locus_tag="NBI42_001722"
                     /pseudo
     CDS             complement(9921..10490)
                     /locus_tag="NBI42_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10977..11858)
                     /locus_tag="NBI42_001723"
     CDS             complement(10977..11858)
                     /locus_tag="NBI42_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1778139.1"
                     /translation="MATQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKMLVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEHAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(11969..13090)
                     /locus_tag="NBI42_001724"
     CDS             complement(11969..13090)
                     /locus_tag="NBI42_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCD1778140.1"
                     /translation="MGLKDQAVAWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLKTGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQPAEELGHGAFKIIETHALQDVQAVLGFHNDPSRPVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLIETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
BASE COUNT         4582 a         2679 c         1895 g         4430 t
ORIGIN      
        1 aataaaataa taaatcgtta caatcgttaa gaagataaca ccaataagca ctgatattct
       61 agtttcaaca ttaaatacca taccgaccaa tactaatatt aagaataata ttgttaagta
      121 gttacttact gcgccaccag gcattttgaa tgggtgacct tccacttctt cagggtgtaa
      181 ttttctaaaa cgtaagtgac tgaataaaat cataaaccaa ggaatcatac ctggtaaaat
      241 agacgcacta tatacgtata cgaaaatact cttcgctcca tcgatatata aaggtaaaat
      301 tacatttaat aaagcaccaa ttaaaatacc aagagatact gcaacaacag tgtacaatgg
      361 cacaccattt ctcatgattt tagtaaatat tttcggcatt tcacctttat gagcaagtgt
      421 ataaatcata cgactcgcac tgaaaatacc tgagttacat cctgacattg ctgcagttaa
      481 tacaacaaag ttaattaatc cagctgcaaa tgtaataccg attttagcaa atgttgcaac
      541 aaacggactt ccaatgtctc ctaattgatt ccaaggatac actgaaacaa taacaaaaat
      601 agcacctaaa tagaaaatta aaattctcca gataacacca ttcactgctt taacgatatt
      661 tttctgagga tctttagttt cacctgcagt aatacctatc agttccacac cttggtatga
      721 accaattaca attgataatg caaagaagaa accaacaata ccatttggca taaatccgcc
      781 atttgtccat agattagaaa tacctaccgc atggccacca tttccaaaac caaagaaaat
      841 aagaccaaaa ccagcaataa tcattaaaat aattgttaca actttaatta aagcaaacca
      901 aaattcaaat tctccaaacg cttttaccga gaataaattc gctgccatta ataataaaat
      961 agcaataata ccaggaatcc aagttggcaa ttccgggaac caaaacttca tatattctcc
     1021 tactgcgatg acctcactca taccaacaac aatccattgg aatatattac tccatgctgt
     1081 catataacct gctgcaggat gtatataatc acttgcaaat gttgcaaatg atcctgttgt
     1141 agggtttaaa taaatcattt cccccattgc tctcatgatt aaaaataaaa agatacccgc
     1201 aattaaatat gcaaggataa ctgatgggcc cgtccattta attgtacttg tcgcacccat
     1261 gaaaagcccc acgccaatcg taccaccaag tgcaatcatt tgaatttgac gggcactcaa
     1321 tcccctttgt agttcattgt tctccataac ttcttacccc atctctgatt gatactaaat
     1381 aaatccataa aaaaacttat tgccgtaatt atactataaa ttatcgattt tggtacatta
     1441 atatttgcat accatgcaat cgtttacaga actactatag caggcttttg caactttgca
     1501 aacaattatt ttgtgatttt tctaaaaaat taaaaaagtt gtctaagtat cggtgagtca
     1561 catacaactt cagctattac aagtaattat gatataattt taataatatt aaaaaggagt
     1621 atatcaatgt taaaacaaat actgccacgt gctacaaaaa tatcggtaat ctttgctgta
     1681 gcatttttca tcataaatta tatcggtatg tacaaacctg acatcctata tttagttgga
     1741 agaactatta ttgcaacact tgctttcata ctcatttgtc ttacattatt tactattatt
     1801 aattctccag agcgcaagat taaactcggt acgactttac cgattgcatt aattattggt
     1861 atcattttcg gcgccatatt tttaacggta caaatcggtg tcatcactgg gttaatcatc
     1921 ggtgtgattg ctacgtttat ttgggaattg attgaaaaaa ataaaggagg tcgttcatca
     1981 tgacaacagc attaattggt gtaattgtat taatcttact aattattagc tttgtgccaa
     2041 actaccgtgc tatgaaatca gcaaaagaac aaggaagtaa tgcaacaaga gctacaatta
     2101 tggtaggcat agatattatc ttaattgtac ttatcatcgt gacgttatta ttaaaatata
     2161 tgtaactatt taaaccaccc tatctcatac ataaacatga acgttctacc atttcacata
     2221 catatggtcg tccatttaat gaggtaaggt ggttttctcg ttaaagttta ccttgtaata
     2281 gtaaaaaata gccaattgtt gcgagtatta agtaaatcat tgaaccaaca acaaaagatc
     2341 ttacttgcac gcgaaatggt gtctgtttgt taaatatagg accaatcacc aaatttccaa
     2401 cgcctaaaat tactggacca gctagaaaaa acaatataat gtaacccatc atgatacgcc
     2461 tcctatttcg taaagaaacg cgctacaatt tgatcgtaat caatatcttt tgctacataa
     2521 tctacattag aaaagtcctg tgcgcttgtc attttattcg tattaacaat aacatctaat
     2581 ccagctgcaa tcgcggctgt tgcaccattt acagaatctt caatagccaa acattgtgtc
     2641 ggcatataat ttaattgttg tactgcagat aaatataatt cagggtcagg tttaacttgt
     2701 tcaacatctt ctctaccaac aactacctct atataatcgt ctagacctaa tgctttaaag
     2761 gtaggcatta tttccgcttt cacactactt gtggcaatcg ccataggaat gtgacgttgc
     2821 tttaaaaatg ccatcaatga tttaatcgta tcaatcatcg gtaatgttgt actagtaaga
     2881 tgatgttctt cataaagttt atctttattt tccgaaccaa tcgctttaat taaatggtca
     2941 tgcaaatctg tagctgctcc cccaatagaa gaacgataaa aatcaatgct tataggatcg
     3001 gcattatgca tctctaaatg tttattaata acattaaata aatgttgttc cgtatctatt
     3061 attgttccat cgaaatcaaa tataactgct ctatacatat atttactcct ctattttttc
     3121 tctttatcaa agcttaacac agtccaatga ccacttcatt ctattagtgc aattataact
     3181 tttttgacca atactttgct aaaataggcc ctgttatatt atgaatgaaa ctaaacactg
     3241 cgcctggtac tgctgcaatt ggattaaaat gcaatgctgc taatgacaca gctaaaccag
     3301 agttctgcat tccaacttca atactgactg ctttttgatc ttgtcgatct aattttaaaa
     3361 gacgagctaa ccaatatcca atcgtatacc ctaatacgtt atgtaaaata actactaaaa
     3421 atattaataa tcctgtagtt aagatttggt gcttactgcc acctacaact attgctaaaa
     3481 ttaatgaaat agcaacaact gatataatcg gcaaagctgt agaagctttt tcagcaattt
     3541 tacgattaat aatttgcaaa acaataccta aagcaattgg aattaataca acttgaacaa
     3601 ctgaccacaa catactcaag aaagatactt ccaaccaatc atttgcaaat agatatatta
     3661 acgcaggtgt aacgaatggc gctagcaacg tagagaccgt cgtaatagaa acagaaagtg
     3721 ctacgttagc tttggctaaa taactcatta cattacttga tgtcccaccc ggacaacatc
     3781 caactaatat tacgccaaca gcaatatcag gtggtaaatg aaaagacttt gcaattataa
     3841 atgctaatgt gggcataata ctgaattgta gacagacacc aataattact gctctaggtg
     3901 ctttaaagac cattttgaaa tcattaggtg taattgttaa tcccatacct aacataacaa
     3961 taccaagtaa ataaggtacc cacttaccta atgtcgcaac atgttgtggg aaaataaatc
     4021 caataacagc tgctactaac atccatacca aaaaactctt cgtggcaaat atactcaatt
     4081 tttgaaacat tgttaacaca tcctcgtcga taatattgaa aaatattacc ataagaccag
     4141 taaggttaca attacttttc tgaaaattaa gattatttag tcattctctt cttattgcaa
     4201 atattttatc actgataaac aattatttta aacttcattc aaaagtctta gtgaataaag
     4261 aaaaaaagcg tggtaaaatc aacttgaaca aaattaagtc acctttataa atattaaacg
     4321 tgtatttaat gccatgtatt cctcgggagg tcctcacatg ttaattgata aattcgaaac
     4381 atacatcatc aacatcgccg gtttaaatga ccgtactact agaaaaaaat taagtaagtt
     4441 atgcaaaagt gtacaatttt gtgatgcgtt gcaattctca ataaacaaac agtttaatca
     4501 gtatgtatta gaaattagct taccaaagca acaacttcca tacttcattt catttttaag
     4561 ttttcatcag tatagtattt tccaagtact ttcaccaaag aaaataaatg aattacttga
     4621 ttctgataat ttatatcaat ctgcaaagcg tttcgatatc aatattgatg gcttgcaaga
     4681 tgctttcatt aaagacaaag tcatcgatat tatgaatatg ttccaaaatc acactgatat
     4741 cacttatacg ttgaataagt ctcatgcgca tattatatgt acgccagaaa tattcgctaa
     4801 attgttacat acgattgcaa ctagaaatat cgacattcta tctgccaatt atagatcgtc
     4861 atctatgagc aaagctcgta tatcataata aaagacacat ctatacgatg atcatttaca
     4921 atgattgcgt atagtgatgt gtcttttttt gtattattta tcttcggacg attgatcctc
     4981 aaccatttgt tcaaatgctt cacgaacttg tggtactgtc atacctacta caacttggat
     5041 actcttacca ctctttacta atccgtgtgc catttgttga tgcgtgaaat attccgtatc
     5101 cgcaacttta ctttcatcat aaacagttaa gcgtaatctt gttgtacagt tagtaacatc
     5161 tttgatattt tctttgccgc ctaaaccgtc taggtaatat gcagctttgt cttcgtattc
     5221 attaccagtt gaagcacgtt tagaatctac gctatcacct tttttatttt tgtaatcttg
     5281 cttagaaaat aatttaactt cctcttctgt tttcttacgt ccaggtaatg gaatatcaaa
     5341 tttcaaaatt aagaatctga ataagaagaa gtaaatcgct acaaatatta agcctataac
     5401 aacttgtgca acataagtgc cccaatgttc tttacctaat ggcaaccagt ttgttgaaat
     5461 aaagtctaac aagccaccac ccatgttacc tacaacacca aatgcgtaca ttaaagtatc
     5521 cattgatgct gctaaaactg cgtgtaaaac aaataagtat ggcgcgatga ataagaatgt
     5581 aaactctaat ggctcagtaa tacctacaac gactgctgtt aatgtggcag gaataagtag
     5641 tgccgccacc tttttacgat tttctttagg tgtcgtagca tacattgcta aggcaatacc
     5701 taaacatcca aatactttcc catttccttg taacataaag ccataaggga attgctcttt
     5761 taatggctta gtactcttag caaactcatt taaatgttgt aaccattcag ctttcaagcc
     5821 gtgattgact actactggtc caacttcaat tggtgcataa ataaagtgat gtaatcctgt
     5881 tggtattaat acacgttcta agaagtgata caaccataca ccgatatagc ctgaagcaat
     5941 gataaagtgt tgcattgagc caataccgtg ctgaacagtt ggccaaataa cacaagtaat
     6001 tgctgctaat ggtaacatca caaaaaatga gattgtaacc acaaatgtta aaccttggaa
     6061 tacaccaacc atttctggta atcgtttact atagtatcta ttgtgaatcc aagtaataat
     6121 agctgaaata ataataccac ctagaatatt cgtatccaat gtggcaatac ctgcaattga
     6181 ttttaaacca ggtacatttt caacgccttt ttctaaatta gcgccaaacg tatgtggcca
     6241 ttgcgttaaa atggcattta taaatgtatt aaacattaag taacccatca atgctgcaag
     6301 tgctgcatga cctggtgctt ttttagctaa agaaagtggt aaacctacaa caaatacaac
     6361 ttccatatgc gtaaaaataa cccagccacc agattctatc actgaccaaa atttaaacca
     6421 aaacgtatgt tgatctgcta aactccccat gattgtagga tttttaaata atgtcgcaaa
     6481 gccaagcact atcccaaaga aagcgaacat taatactggt acaatcattg cactaccgaa
     6541 acgctttatc gcattcatct tctattccct ccatatcatc tttcctaaca atacatctaa
     6601 ttagattcat ttataaatag atgtcttact atttaaatat aatatatagt aaacgcttac
     6661 acacctacaa cgacattgac gtattttgaa agtattttgt ataatcagat tatcttttca
     6721 tatagtgaaa attttttcac gaccttatat ttgacatcgt tgtatttgta atacattcgt
     6781 tttaaacgca taatcaaacc tatatcaata cacaaatata tataatgaca tacaaggttt
     6841 taatgtaata acaatctatc aaacatttat tttcaaggag gttgaatatg tttttagatg
     6901 aacacattaa tcgaaacttt gataaactta atgacaatga tttacatatc gctcacttta
     6961 tcaatacaca tatagatgaa tgtaaaaata tgaaaataca agatttagcg caattcacac
     7021 atgcttcgaa tgcaaccatt cacagattta cacgcaaatt aggttttgac ggttatagtg
     7081 attttaaatc gtaccttaaa tttgaaagta ataagacaca ccaattacca tctaactcta
     7141 tcgatagttt caaacaagaa atcgatagta cattcagtta tttagaacgc gttgattacc
     7201 agcttcttac tgaaaaaatg tatacagctt caacaatata tttgtatggt acagggcgtg
     7261 cacagttaaa cgttgcttca gaggcacagc gtatattgtt aacgttacat aatcatgtca
     7321 ttgtacttca tgatgaacat gaacttaaaa tgattttgaa caagagtgaa cctaatgatt
     7381 tattctttat tatttcatta tcaggtgaaa cacatgaatt attagaagta acaaacttgc
     7441 ttcaacttag acaaaaatac tttatttctg ttactacaat gaaagataat acccttgctc
     7501 aaaaagcaaa ttacaatgtc tatgtatcca gtaacacatt ctacttaact gatggtacag
     7561 actattctag cttcatcagt tatcatatat tctttgaaac acttgtaaga aaatataatg
     7621 agtatttact acataatgat atttcaaatt agaaaaaagg aagttatgat ttaatggcct
     7681 tgagcctatc ataacttcct tttatcattt tattgttgtg ttgatgtttc gataacgtgg
     7741 tacatcttat caaacatcaa ttcgaaacca tgcaccatgg catcatgata ttcttttttc
     7801 ttttgcttgt attctaaatt agtaaatcgt ctttcttttt caactaatga acgataataa
     7861 aatagcattt gggtgccacc tgtttcacgt tcaaaaaatt ctacctcaat gacatcttgt
     7921 gtttcactta gtccaggcat accgatagtc atcttaacgt attcatccat aactaaagat
     7981 tcataaatac cttcaatcac atttactttg ccattacgtt gttgatctac aatacgatat
     8041 ttaccgcctt ctttaacatc cgctttaatc tctttattcg ttctggctga tgtcataaac
     8101 cattgtttca acaaatcttt ctttgtccaa gcttcgtata ctaactctgg agaaaattta
     8161 taaagctttt caatttcaac ttcgacatgt tcattctcta cattaaattt tgccactgtt
     8221 gtccacccac tttcgctctt acttttattt taacgtattt ttgctcagtt ccaaacatag
     8281 atgatcatca tttttaaaag attagcgtta tacggtgagt acaacatgat ctgttaatat
     8341 aacaagccac cttacttggc tacatcgata tattgttaag cattaatgtt tcatttcttg
     8401 actagtgttc ttttttagct ttggaaaatt aaataaaatc gcaataagtc cgcatacacc
     8461 taataatata ggataaatgc tgtatgggaa taacattaac ggtgatatac cagctacacc
     8521 agccgctgaa atgacttgcg ggctatatgg taataaacct tggaagcagc ctccaaatat
     8581 atcaagaata cttgctgatt tccttgaatc cacatcatat tcatctgcaa tatttttagc
     8641 taaaggacct gacataataa tagagatggt gttgtttgcc gtggcaatat ctgcgacact
     8701 taccaaactc gcaattccta attctgcgcc acgctttgat ttcactttag agcgaacaaa
     8761 ttgcaacaac cattcaatac caccattgtg ttgaataata ccgactaaac caccaattag
     8821 caacgcaatc atagcaatat cttccatgct tataatacct ttggacactg catctagtag
     8881 ccccatccaa ccgaatgaac catctatgag accaatgatt ccggctaata atgttccgcc
     8941 aatcaatacg ataatgacat ttacacctaa taatgctaat accaatacta aaatatacgg
     9001 tacaacttta attagattgt aatcatagtt tttagcatga tttaaagaaa tgccattcgt
     9061 taagaaatac agaataataa tcgttaaaat agcacctggc aatacaattt taaagtttac
     9121 tctgaattta tctttcattt tcgtatgttg tgttctaacc gcagcaattg ttgtatctga
     9181 aatcattgat agattatcgc cgaacattgc acctccaaca actgtagcca ttgctagtgc
     9241 agttggtaca tctgtcgctt gtgcaaatcc gtaaccaaca ggtgctattg cagcaacagt
     9301 ccctactgac gttcccatag atatagaaac aaacatacag atcacaaaca atcctacaat
     9361 aattaaattt tcagggatta atgatagtcc taaattaact gtcgacttta cgccacccat
     9421 tttttcagct gtatttgaaa atgcacctgc taaaataaaa atcaacatca ttaaaataat
     9481 gtttgaatgg cctgcacctt tcgtgaagac ctcaactttt ttagcaaatg attcttttcg
     9541 attcattaat aacgccacaa ttaccgttat cgtaattgca acatttaatg gcattgaagt
     9601 aaaatcacct gtgataatac ctacgcctaa aaacaacgcc acaaataata acaaggggaa
     9661 taatgcccaa gcattgctct ttttatgtac ttccatcctt tttacctgct ttccaattaa
     9721 aaatacctct ttctcacaaa cgatgaagaa agaggttttc atgtgcttta cctgcttatc
     9781 ttcaaaccat tacggttact ggaattggca cattcgagat gttgccgagg cttcataggg
     9841 ccagtccctc cacctctcta gataagtgat gcttatttac gtttacgtta caagataatc
     9901 cttagtacgt caatcataaa ttaatcagga gtcgtataat atttttcata aacaatcatt
     9961 gctactataa taataatcaa aacaataatg ctaataacaa gtaaaagcca ccatttaagc
    10021 attaatgcaa taaaaatgaa cacgatagac acacttacta atattaatga tatgacttta
    10081 aattgctgtg cgctttgttc agaaataact ttcagctgtt tgtttgatgg acgcgtgttt
    10141 cttatactga ttcccagtat attttctaat atttgaacca atacgatact tattgcaaat
    10201 ataataattg gtaaaacatc atagctccct atagttaatg tataaattac aaatccaatg
    10261 taaagtaacc ctgagacaaa ggctaaaaag tatgcgacgt atttgttaaa cttaatgata
    10321 ttctttttaa cgttttgatg tgtaagctat atattcgaaa caatcgcaac cactacaaac
    10381 agtgtgaata atatatataa tggcaacttt tgttcaggaa aaacagtcgc tattccaaaa
    10441 gctaatgcta aaatcaaaaa taatatagct ctatatacta ttaatgccat aataacaacc
    10501 cctttgttta atattgagtt tgcaaattta cgtttatcag catttctatg attagtactt
    10561 ctacgggcag cgtttctatg taatttacat catcttaaca tataaatact tcgctatata
    10621 attgaaaaca tatcctatta ttctttgtcc gttctgacgt ctaatatcta accttaggca
    10681 tttcacttgt taatgaattt aactttcttc cactaaccgt ccctaaaccc aatcccgcaa
    10741 cagtttttaa ctttttcgtt gttgtcctga catcctcatt aagaaagttt attctgctta
    10801 aaacttataa tccacaccct gagcaaacgc tccttatgac aaagtattaa aataagccga
    10861 taaagataca cacctttacc gactatttaa aatacacttc actaattcat tttaatttac
    10921 ttgattgaag tggctaaatt aatattatgt tgttcaatta aaagcttcat acaaacctaa
    10981 tctatttgca ctccaccgct aacaccgaac acttgtccag ttgtataact tgattcttct
    11041 gacgctaata gcacataagt tccacataac tcaacaggtt gacctgcacg acctaaaggt
    11101 gttttttgac caaatgttgg gattttactt tgaggttgtc caccagaaat ttgtaatggt
    11161 gaccagaatg gaccaggcgc tacacagttc actctaattc cttttggtcc taattcttct
    11221 gaaaaacttt tagttaatga aataattgct gcttttgaag cggcataatc atgaagaata
    11281 ggactaggat tataaccttg tacagatgat gtcgtcgtaa ttgacgcacc cggttttaaa
    11341 tattccaaag ctttttgaac tgtccaaaat agcggataga cattcgtttc aaatgtttct
    11401 gtaaatgcct cagttgtaaa tccatgaata tcatcatgat actgttgatg tccagcaact
    11461 aaagtaacat tatctaagcc acctaattgt tgatatgcat gttcaacaag gtcatagttg
    11521 aactgctcat ctcttatgtc accaggaatt aacactgcct tttgaccact ttcttcaatc
    11581 acttggcgta cttcttgtgc atcttgttct tcgctcggaa gatagttaat cgctacatct
    11641 gcaccttctt tagcatacgc aattgctgct gcacgcccta ttgctgagtc accacctgta
    11701 actaacattt tatagccttg taagcgttga tgaccttggt aagacgtttc gccacaatca
    11761 ggtgctggcg tcatttcaga ttgtaaaccc ggtacctctt gttcttgttt ttcataatcc
    11821 gttgttttaa attttgttct aggatcttga gttgccattt tttaacatct ccttattcgc
    11881 ttaatgatta ttatttaccc aatcttccta ggaacttaat catgattaca ctaaaaatta
    11941 ctttcttctt tataaaaaaa agctcgaatt actcatgcaa tagtctcttt acaaattcaa
    12001 caaaatactc cggtactttt tccagaatcc tttcatccgg tttatattga ggatgatgta
    12061 aatcatattc actatgagaa ccaattaacg caaatacact tggaaaatgt tgactataac
    12121 ctgaaaaatc ttctccaatc gtaagcggct gttccatcat ttccacctta tatccaacat
    12181 gttgggctac tgctattgct ttatgcgtca atgcctcatc attcatcaca gcgccaggta
    12241 aatgtgtata atttaaatta attttcatat tatatgcttg agctaacccg tccgcaatat
    12301 cttgtaatcg tgtttctata agctttcgca ccacaggatc aaaactacgc actgtgcctt
    12361 gtacatacgc atgatcagca atgacattcc aagtattacc acatgatatt tgcccaattg
    12421 ttactaccgc ttcatcaaac gcagatagat ttctactaac tatggattga atactattaa
    12481 tcaattgcgc caacacaata actggatcat tacattgttc tggctttgca gcatgaccac
    12541 ccacgccttt aatatgaaac tcaaaacgat ctactgctga tgtaattgcc cctgttttga
    12601 ttgcaaatgt acctaccgga cgcgatgggt cattatgaaa acctaatacc gcttgtacat
    12661 cttgtaatgc atgtgtttca ataattttaa aagcgccatg tcctagttct tctgctggtt
    12721 gaaaaatgaa tttaacacgc ccagtaagag tgccctcaat ttcttttaac tttacagccg
    12781 tagccaaaat actagccatg tgaatatcat gaccgcacgc atgcataaca ccttcatttt
    12841 cagatttaaa atcctgttca acaagctctt gaatcggtaa ggcatcaatg tcagcccgaa
    12901 cagctataca actcagacct tgtcccactt cagcaaccag cccagtcttg agcggtaagt
    12961 ctaatattct aatatgatgt tctgttaaaa tatctttaat tttttgtgta gtcttaaatt
    13021 ctttatcgga tagttctgga aattgatgaa aataccttct ccaggcaaca gcttgatctt
    13081 ttaatcccat cggtcattcc ccttccttaa gtcaatgaaa tgttgtctac cctacgatga
    13141 tcgtctttaa ctatcaaacg atgatttcac aacaatgtac tcttgttaat tactttcgct
    13201 aatgatagac aattgtttaa taatatcgta acactattgt caaactattc taacttttat
    13261 aattaagaat ctatacaaaa acgtgttctc ggataaactt gtttttacaa atcacaaaaa
    13321 gagtctggga cataaatccc taaaaaaaca gcagtaagat aattttcaat tagaaaatat
    13381 cttactgctg ttctctattt atacaatact tcgtattgaa tggcttcgct ttcctagggt
    13441 gccgtctcag ccttggtctt cgactggcac tgcttcctca ggagtctcgc cattaatact
    13501 acgtattaac atgtaatttt actttgaaat actttaaaaa aataagacac tttcgtataa
    13561 tttaataaat accactaaac taaatt
//
LOCUS       DAHHRR010000070        13510 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.070, whole genome shotgun sequence.
ACCESSION   DAHHRR010000070 DAHHRR010000000
VERSION     DAHHRR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13510
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.070"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(739..3585)
                     /gene="uvrA"
                     /locus_tag="NBI42_001725"
     CDS             complement(739..3585)
                     /gene="uvrA"
                     /locus_tag="NBI42_001725"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCD1778141.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIEGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNINLSE
                     QDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASNRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKLKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            complement(3593..5578)
                     /gene="uvrB"
                     /locus_tag="NBI42_001726"
     CDS             complement(3593..5578)
                     /gene="uvrB"
                     /locus_tag="NBI42_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCD1778142.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLIMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNDRV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            complement(5847..6083)
                     /locus_tag="NBI42_001727"
     CDS             complement(5847..6083)
                     /locus_tag="NBI42_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HCD1778143.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            complement(6080..6730)
                     /locus_tag="NBI42_001728"
     CDS             complement(6080..6730)
                     /locus_tag="NBI42_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1778144.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            complement(6904..7743)
                     /locus_tag="NBI42_001729"
     CDS             complement(6904..7743)
                     /locus_tag="NBI42_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1778145.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKVVKSPVRKASQAKKVVKQSVQQASKKVVVKQAPKQAVAKAVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            complement(7891..7998)
                     /locus_tag="NBI42_001730"
     CDS             complement(7891..7998)
                     /locus_tag="NBI42_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778146.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIIENVLNYMI"
     gene            complement(8152..9262)
                     /gene="prfB"
                     /locus_tag="NBI42_001731"
     CDS             complement(join(8152..9189,9191..9262))
                     /gene="prfB"
                     /locus_tag="NBI42_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCD1778147.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            complement(9417..9533)
                     /locus_tag="NBI42_001732"
     CDS             complement(9417..9533)
                     /locus_tag="NBI42_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778148.1"
                     /translation="MLWEESFYFTSVKYFKKYEEVVKRCYVLGFKKGLGICR"
     gene            complement(9575..12106)
                     /gene="secA"
                     /locus_tag="NBI42_001733"
     CDS             complement(9575..12106)
                     /gene="secA"
                     /locus_tag="NBI42_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCD1778149.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            complement(12520..13092)
                     /gene="raiA"
                     /locus_tag="NBI42_001734"
     CDS             complement(12520..13092)
                     /gene="raiA"
                     /locus_tag="NBI42_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCD1778150.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(13153..>13510)
                     /locus_tag="NBI42_001735"
     CDS             complement(13153..>13510)
                     /locus_tag="NBI42_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCD1778151.1"
                     /translation="AHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPIEAVLKAKGIRFD
                     KILKMSNRPKQSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCK
                     LYAKNIRKFKVFAFAR"
BASE COUNT         3949 a         2670 c         1886 g         5005 t
ORIGIN      
        1 tccataagtt gccaactagt tcgttgtttt ctgcatgatg taaatctata acctccattc
       61 tttactttgt atgaatattg ttcagtcctt cggtacattt tccctactat gacgtctgct
      121 gacttctcat tattcgttgt tactacggta tgtgtaccgc tagtgagacc tccctgggta
      181 aggtgcaacc actttccact catctcatcg catcatttac catataaaat tcgggtagta
      241 ttggacttcg tcttgtttag cagactcatc cgttttatac ggccttatta tgatgtttct
      301 gttcgtcaat gcgagtgttt gcgtccgact tccttcagat tccacttcac aatggacacc
      361 cttgtctttc gctaacagtt cctactacca agcctgtaac ggactttcac cgtcaagttg
      421 ttgcccatgc caggcgcact aaaaaatagc tatgaaaaaa tctatcgtca tagatttctt
      481 catagctaat cttagtatgt ttcctatttt taagtacaca atagctgtgg ctaatgacaa
      541 agaaccacta cataataaat cgatagtgat tctttaccat ttctgtccca ctctcagttt
      601 cgatttatgt ttagctactc tagattgacc ctatcttaac ataattcttt ttacgcatct
      661 tttctttcta ctattcatta ctaaattaac aaaaagaagt gaagggtaaa tttacaacac
      721 ccttcacttc ttttaatctt atttatcttc agtattttgt ttatctcgtt caagtacttc
      781 ttttaaatac tttcctgtat atgatgactt tgtctgagca atatcttcgg gtgtgccagt
      841 cgcaacaata gtaccaccgc cactaccacc ttcaggacct aagtctataa tatagtctgc
      901 tgttttgata acatctaggt tatgttcaat aattacaaca gtatcaccat tttcaactaa
      961 tcggtttaat acttttaata atctactaat atcgtcaaca tgtaaccctg ttgtcggttc
     1021 gtctaggata taaatagatt taccagttga acgtttatga agttcagatg caagtttcac
     1081 acgttgagcc tcaccacctg ataacgttgt agcttgttga cctaatgtga cgtatccaag
     1141 accaacatca actagtgttt gtaacttgcg cttaatctta ggaatatttt caaaaaattg
     1201 tgttgcttct tcaacagtca tttctaaaat gtcagcaata tttttacctt tgtaagtaac
     1261 ctctagtgtc tcacgattat atcgtttacc atcacacact tcacaaggaa cataaacatc
     1321 aggtaaaaaa tgcatttcaa ttttaataat accgtcacct ttacaagctt cacagcgtcc
     1381 accttttaca ttaaaactaa aacgcccttt ttgatatcct cgaattttag cttcatttgt
     1441 ttgcgcaaac acatcacgta tatcatcaaa cacgccagta tacgttgctg gattagagcg
     1501 tggcgttcta cctatcggtg attgatcaat atcaataatt ttatcaagtt gatcaatacc
     1561 ttcaatctta tcgtacaatc ctggctttaa tttagattta ttgatttttt gagctaatga
     1621 tttgtataat acttcattta ctaatgagct tttaccagaa cctgatacac ctgtaacgac
     1681 cgtcatgatt gaaagtggta tgtccacatc aacgccttta aggttgttac ttctagctcc
     1741 acgtatagaa attttacgat ttgaagccgg tctgcgatat tcaggtactt caatacgttt
     1801 cttaccactc aagtattgtc ctgttaaaga ttttttatct ttcattacct tttgaggtgt
     1861 accactagac acaatctgtc ctccatgctc accagcacca ggccctatgt ccactaagta
     1921 atcagccgca cgcattgtat catcatcgtg ttcaactaca attaaagtat ttcctaaatc
     1981 tctcatttct ttaagtgtat taattaatcg atcattatct ctttgatgca gtccaattga
     2041 tggctcatct aatacatata agacaccagt caaacgtgac ccaatttgcg ttgccaatcg
     2101 aatacgttgt gcttcaccac ctgaaagtgt acctgaagct ctgtttagcg ttaaatattc
     2161 aagtcccaca ttatttaaaa aagtgagtcg ggaaataatt tctttcaata tttgatttgc
     2221 aatcgcttga tcttgttctg acaaattaat gtttttataa tagttcagcg cttgactgat
     2281 tgagtattcg actacttcac caatatttaa accacctaca taaacagata acgcttcacg
     2341 actcaatcgc tttccatgac aagtttcgca aggtagttcg gtcatatatt tactcatcat
     2401 ttcacgtgta tattctgatg gagattcatg gaatcgtcta cttatattag gaactacacc
     2461 ctcgaaaacc attgttcgtt ttctagtacc accttgacgt tgtgtaaatg taaattcaat
     2521 ttctttgtca cccgaaccat acaataaaat atcacgttga cgttctgtta actttttaaa
     2581 aggtttatcc atattgattt tataaacttc acaaacacgt tttaacaatg ttggataaaa
     2641 atcagaactc gtcggtatcc atggttctat tgcaccttcg tttagcgtct tatctttgtc
     2701 aggaacaacc aagtctacat cgactgttaa cttttgacct aagccatcac atgttggaca
     2761 agcaccaaaa ggactgttaa agctaaacat tcttggttct aactctccga ttgaaaatcc
     2821 acatatagga caagcatggc tttctgaaaa cttaaggtct tccccttcaa taacatcaac
     2881 tgttaattgt ccttctgaaa gctctaacgc agtttcaata gagtcagcta gtcgtgtttc
     2941 aattccatct ttaacaacta atcggtctac aacaacttct attgtatgat tcttgttctt
     3001 atctaatgta ggtacatcat ttacatcaac aatttcgcca tcgattctta aacgtacata
     3061 acctttttta ccaatatctt cgattagctt ttcatgacta cctttacgat gagcgatgac
     3121 aggtgctaat aattgaatct ttgtacgtgc ctctaattcc ataatgcggt caaccatttg
     3181 ttgtactgtt tgcgattcaa tttctatatt gtgatttgga cagtaaggtt taccaacacg
     3241 tgcatataac aaacgtatat aatcatatat ttctgttact gttgcaacag ttgaccttgg
     3301 atttttactt gttgtttttt gatctattga aattgctggc gataatcctt caattgtatc
     3361 aacatctggt ttgtccattt ggcctaaaaa ttgacgcgca taggcactta atgattcaac
     3421 ataacgtcgt tgtccttcag catatatagt atcgaatgct aatgacgatt tacctgaccc
     3481 agataatcct gtcataacaa ttaatttatt tttaggtagt tcgatatcaa tatctttcaa
     3541 gttatgcgca cgagcacctt ttactactat ggatggttct ttcatttact tgtcaccctt
     3601 ctgcttttaa ttcaaataac atatctctta attctgtagc tttctcgaaa tctaaatctt
     3661 tcgctgcttg tttcatttct ttttctatat tgtcgattgt cttttgacgt tcttttttcg
     3721 tcatcttctt aggtatcaca gtttgtgctt tgtcattatt ttcgtcattt tcaacagtag
     3781 cactaattaa atcatgtatc tttttattaa ttgttttagg cgtaatacca tgtttttcat
     3841 tatgtttcat ctgtatttct cgacgacgtt gtgtctcatc aattgcatac ttcatcgaat
     3901 cagtcatttt atcggcatac ataatgactt cacctttatc gttacgcgca gctctaccta
     3961 ttgtttgaat taatgagcgg ttagaacgta aaaatccttc tttatctgca tctaatatga
     4021 caactagaga aacttctggt atatcaatac cctctcttaa taaattaata cctacgataa
     4081 catcatatgt acccattcgt aagtctctaa ttatttcgat tcgttctaat gttttaattt
     4141 cagaatgtag atagttaact ttaatgcctg cttctttcat atatgtggtt aaatcttcac
     4201 tcatcttttt agtgagcgtt gtaacaagta ctctatcatt acgctcaacc cttgtttgaa
     4261 tttcacttaa taaatcgtca atttgatttt cagtaggtct aacctcaatc ttagggtcca
     4321 gtaaaccagt aggacgaata atttgttcaa ccatcttatc cgtatgttca atttcgtatg
     4381 gaccaggcgt tgcagataca tacacaagtt gtttcgtctt ttcttcaaat tcttcaaatt
     4441 taagtgggcg gttatctaac gcacttggta atctaaatcc atgatcaact aaaacttgtt
     4501 ttcgtgctct gtctccgtta tacatgccac gaacttgcgg taacgtcaca tgtgattcat
     4561 caatcattat taaccaatca tcgccaaagt aatccaataa agtatatggt gtcgaaccta
     4621 gtggtcgcaa cgttaaatgt actgaatagt tttcaattcc tgaacagaaa cccatttctc
     4681 gcatcatttc taaatcatag ttggtacgct gttctaacct ttgcgcttct agtaatttat
     4741 tctcatctcg taattctttc aatcgttctt ccaattcttt ttcaatacgt tcaatcgcaa
     4801 ctttcaactt ttcttcacgt gttacgaagt gagaagctgg gaatatcgca aaatgttctc
     4861 tttctttcaa cacttcacct gttaggtagt taacttctcg aatacggtca atctcatcgc
     4921 cgaaaaactc aacccttata caaagttctt ctttagaggc tgggaatatt tcaactacat
     4981 caccacgcac tcgaaacgtt cctcgttgga aatcgatgtc attacgtgta tattgcacat
     5041 ctacaagttt tctaagtaat tcgcttctat ccatttccat accaactcga acacttacta
     5101 ctaaatcttt atattcttca ggattaccta aaccatatat acaacttaca ctagcaataa
     5161 taattacatc atcgcgttca aataatgcac ttgtagcaga atgtcgtagt tgatcaattt
     5221 catcattgat tgaggcatct ttttcaataa aagtgtcagt agacggtacg tatgcctcag
     5281 gttgataata atcatagtaa cttacaaagt attccaccct gttttcagga aaaaattctt
     5341 taaactcact atataattgt cctgctaatg ttttgttatg tgcgataatt aacgttggtt
     5401 tcccaacttc tttaataaca ttactcatcg taaatgtttt ccctgtgcca gttgcaccta
     5461 ataaagtttg atgtcttttc cccgctttaa taccttccac gatttcctta attgcttgcg
     5521 gttggtcacc ttgaggctca aaatcagaat gtattttaaa aggataatgt tcaaccattg
     5581 tcacatatgc ctcctctttc aacgtattat ttatatttcc gctcaactct tcgctttctc
     5641 attacatatt ttagcaaagt agtcacacaa aaagcaaacg tttgttcgta aaaatgtcga
     5701 acaaagaaaa acaaacatag ttgtgctaac tatgcttgca ttaaatcaaa tcgtttatct
     5761 tgttattttt agtatgtaaa tacacattat attaatcatc gttttgtttc aacaaatttg
     5821 tttgaaacat tattttaaag ttaatcttag cgatcttcat cttgatgttt atgaaattcg
     5881 agttgatcta taattaaata accagctaat aatgacacta catcaataaa aataatccac
     5941 tcgttatgga aatactcttt atagattgag gcaccgatta aaattaatgt cagaatagta
     6001 ccgacccatt tacttcttgt tattacacta aacaatacta ccaggacaca tggaaagaat
     6061 gctgcgctaa aataccatat cattcatttt cctcttttct tttatttaaa atgttcatgg
     6121 ttgtttctct taattctgtt ctaggtataa agttttccgt caacatttct ggaatgatat
     6181 tattaataaa atcttgtaca gatgctaaat ggtcaaattg aataattgtt tctagactca
     6241 tttcataaat ttcgaaaaat aattcttcgg gattacgttt ttgtatttct ccaaatgttt
     6301 cataaagtaa atcaatttta tcagcaactg aaagtatttg gccttctaat gaatcatctt
     6361 taccttcttg cagtcgttgc ttataaacat ctctatattg taatggaatt tcttcttcaa
     6421 taaaggtctc taccatttct tcttcaactt gcgaaaataa tttttttaat tcactactcg
     6481 catatttaac aggtgttttt atatcaccag taaacacttc agcgaaatca tgatttaatg
     6541 ctttttcata taagcttttc caattaatct ttcgtccatg atattcttca actgttgcta
     6601 aatattgagc aattttagtt actttaaagg agtgtgctgc aacattgtgt tcaaaatatt
     6661 taaattttcc aggtaatctt ataagtcttt ccatatctga taatctttta aaatattgat
     6721 gtacacccat ttcaattacc tcctccatta attaatcata aattatactt tctttttaca
     6781 tatcattcaa ttaaatatca tttaaatatc ttctttatat aactctgatt aaatgatacc
     6841 aaaaaatcct ctcaacctgt tacttaaaca ggctaagagg atagtcttgt cttgatatat
     6901 tacttagtgg atgtaattat attttcctgg atttaaaatt gttcttgaag atttaacatt
     6961 aaatccagca tagttcattt ccgaaacagt aattgttcca ttagggttta cagattcaac
     7021 aacaccaaca tgtccatatg gaccagcagc tgtttggaaa atagcgccaa cttctggtgt
     7081 tttatctact ttaaatcctg caacttttgc tgcgtaattc cagttatttg cattgcccca
     7141 taaacttcct atacttctac ctaattgtgc acgacgatcg aaagcataat atgtgcagtt
     7201 tccataagca tataagtttc ctctgttagc aactgattta ttgtagttat gtgcaacagg
     7261 tacagttggt actgattttt gtacttgagc aggtttgtat gctacattaa ctgccttagc
     7321 tactgcttgc ttaggtgctt gcttaactac tactttttta gacgcttgtt gtacagattg
     7381 ttttactacc tttttagctt ggcttgcttt tcttactggt gatttaacca ctttagtttg
     7441 tttcacttta ttttgaggaa caagtgaaat cacgtcacca ggaaaaatta aaggtgttac
     7501 accaggattg tatgaatata attgattcaa cgttaagtga tgctctaaag caattttata
     7561 taatgaatcg ccagcaacta ctgtataagt tgtcggtgat tgcgtttgtg cttgaacatt
     7621 tgatacataa ttatgttgaa caggtgtttt tacttgtgtg ccatgttgtt gtgcatgtgc
     7681 tgcattattt aaagctaaaa aagctaacac tgacgaaacc gtcactgtaa gagatttttt
     7741 catcttgctg tcattccttt gctgttagta ttttaagtat gcaaatacta tagcacaata
     7801 cattttgtca aaagctattg ttataacgat gtaatcaaat ggttaacaat ataaaaagaa
     7861 tacaaccttt tatcatagtg taaaatgtat tcatatcatg taattgagaa cgttttcaat
     7921 aattaattca ataccttgaa aatcgccata ggtaatatta ctaaatgcac actgcatatg
     7981 ttgttttaac aaacacaact tttaaaaaat atattctaac cctatcaacc gaattgtact
     8041 taaatattca taaacaaatc atattccaaa atctaattta caatttattt agctaccttt
     8101 aaaaaaacca aaaaccgatg cccttttaga gcctcggttt taaaatatat attaatcgtg
     8161 cgacattgtc tgtcttaaat atgattcgat aaatggtcca atgtctccat ccatcactgc
     8221 atcaacctta cctgtttctt cgtttgtacg atgatctttc accattgagt atggatggaa
     8281 aacatatgat ctaatttggc ttccccatcc gatttctttt tgttcgccac gaatttcagc
     8341 catttcacgt gcctgctctt ccaattttaa ttgatataat ttagacttta acattttcat
     8401 agctgcttca cggtttttaa tttgagaacg ttcattttgg ttattaacaa ctatacctga
     8461 ggggtggtgg gtaattcgta ttgccgattc agttttgtta atatgctgac cacctgcacc
     8521 agaagctctg aatgtatcaa ctgtaatatc atccggattg atttcaatct ctatttcatc
     8581 attattaaaa tctggaatca cgtcgcatga tgcaaatgat gtatggcgac gtcctgatga
     8641 atcaaatgga gaaattcgta ctagccggtg tacacctttt tcagctttta aataaccata
     8701 agcattatgt cctttgatga gcaatgttac acttttaatc cccgcttcat ctccaggtaa
     8761 ataatcaaca gtttcaactt taaaaccttt cttctcacaa taacgttgat acattctaaa
     8821 tagcatatta gcccaatctt gagactccgt gccacctgca ccaggatgta actctagaat
     8881 tgcgttattg gcatcgtgag gcccatctaa taataattgc aattcgtatt catccacttt
     8941 agccttaaaa ttaatgacct cttgctctaa gtcttctttc atttcttcat caaattcttc
     9001 ttgtaataaa tcccaagtgg catccatgtc atctacttct gcttgtagtg ttttataacc
     9061 attaactatt gcttttaacg cattattttt atctataata tcttgcgctt ttgtttggtt
     9121 atcccaaaaa ttaggttctg ccatcatttc ttcatactct tgaatattag tttctttgtt
     9181 ctctaagtca aagagacccc ctaatttgtg ttaaatcttg attatactta tctatatttc
     9241 gtttgatttc tgataattcc atagccttcg ctcctattta taattcaatt caagtcattg
     9301 atttgtgtct tttataatgc taaattttaa cataattttg ttaaataaca atgttaaaaa
     9361 atataagcac actgacaatt agtttatgct tttattgtta aaaatcagta catttatcat
     9421 cgacatatgc ctaaaccttt tttaaaacca agtacataac aacgtttaac aacttcttca
     9481 tattttttaa agtatttaac gcttgtaaaa taaaaagact cctcccataa cacaaactat
     9541 aggtgtttaa ttggaaggag ttattttata tcatttattt tccatggcaa tttttgaatt
     9601 ttttaccact accacatgga caatcatcgt tacgaccaac ttgatcgcct ttaacgattg
     9661 gtttcggttt cactttttct ttaccatctt cagctgaaac gtgcttcgct tcaccaaact
     9721 ctgttgtttt ttcacgttca atattatctt caacttgtac tacagatttt aaaatgaatt
     9781 tacaagtatc ttcttcgata ttttgcatca tgatatcaaa taattcatga ccttcatttt
     9841 gatagtcacg taatggattt tgctgtgcat atgaacgtaa gtgaatacct tgacgtaatt
     9901 gatccattgt gtcgatatga tcagtccaat ggctatcaat agaacgaagt aaaatcatac
     9961 gctcaaactc attcatttgt tcttctaaga tatctttttg actttgatat gctgcttcaa
    10021 tcttagccca aacgacttcg aaaatatctt cagcatcttt acctttaata tcatcctctg
    10081 taatgtcacc ttcttgtaag aaaatgtcat taatgtagtc gatgaatggt tgatattcag
    10141 gctcgtcatc tgctgtatta atatagtaat taatactacg ttgtaacgtt gaacgtagca
    10201 ttgcatctac aacttgagag ctgtcttctt catcaataat actatttctt tcgttataga
    10261 taatttcacg ttgtttacgt aatacttcat cgtattctaa gatacgttta cgcgcgtcga
    10321 agttattacc ttctacacgt ttttgtgctg attctacagc tcttgatacc atttttgatt
    10381 caattggtgt agagtcatct aaacctagtc ggctcatcat cttctgtaaa cgttcagaac
    10441 caaaacgaat cattaattca tcttgcaatg ataaatagaa gcgactatcc cctttatcac
    10501 cttgacgtcc agaacgacca cgtaactggt catcaatacg acgagattca tgtcgctctg
    10561 tacctattac tgctaaaccg cctaattcct ctacgccttc acctaatttg atatctgtac
    10621 cacgaccagc catgttagtg gcaatagtaa cggcaccttt ttgtccagcg cctgcaacaa
    10681 tttcagcttc acgttcatga tttttcgcat ttaacacatc atgacggata ccacgttttt
    10741 taagtaaatt tgaaatatat tcagaagtct caactgcaac agtacctaat agcactggtt
    10801 gccctgcctt gtgtttttca acaacatctt ctactactgc atcaaattta cctttttggc
    10861 taatgtaaat taaatcagac ttatcgttac gttgcacagg tttatttgtc ggaatttgag
    10921 ttactgtcat gttataaata tttctaaatt cttcttcttc agttttagct gtacctgtca
    10981 tacccgcaag tttattgtac attctgaaat agttttggaa tgtaatagac gccatagttt
    11041 tagattcatt ttgaatttga acgccttcct tcgcttcaat agcttggtgt aaaccttccg
    11101 agaaacgacg gcctggcatt gtacgtcctg taaattgatc gacaattaat acttcgccat
    11161 caacaaccat atagtctacg tcacgttgta atgtaacgtg cgcacgtaaa gctgtgttga
    11221 tatgactaat aacatcaaca ttttgtacat catataaatt ttcaactttg aacatacgtt
    11281 cagctttgtc cgcaccttgt tctgttaaat gtacggcttt cgttttttca tcgtatttat
    11341 aatcttcgtc ctgttttaac attttcgcaa aaacatttgc ttgtgtataa agtgacgttg
    11401 acttttcagc ttcaccagaa ataattaatg gcgtacgtgc ctcgtcgatt aaaattgagt
    11461 caacctcatc aatgattgca aaatgtaatg gacgcattac tctatcttca gaataattca
    11521 ccatgttatc acgtaagtaa tcaaaaccta gctcattatt agtactgtaa gtaatgtctt
    11581 gtgcgtatgc ttcacgtttt tcttctgtcg tcttactgtt taagtttaat ccgacagtca
    11641 aacctaagaa gttatataac tcagccattt cttcactttg aacacttgat aagtattcat
    11701 tgactgtaat aacgtgaaca cctctaccag ctaatgcatt taagtatgtt ggcattgtcg
    11761 ctgttaatgt tttaccttca cctgttctca tctcagcgat atcaccttta tgaattgcaa
    11821 taccacccat aatttgaact ttatatggtg tcatattgaa tacacgttta gagccttctc
    11881 taacaagtgc atatgcttct ggtaaaattt tatctaaata atcattttgc tttttgacat
    11941 tatcaatgtc agctaattct gtttggaatt gtttcgtttt attacgaatt tcttcatcag
    12001 ttaaaattgc cgttttttct tctaaagcga ttactttatc agcaagttta cctaactgtt
    12061 taatttcttt attattgcca tcaagaattt ttgataaaaa tcccatttcg ttcgctcctt
    12121 tagctaaaaa actgtttggc ctacaacaat atatcttatc atttatagtt agaaaattat
    12181 acttatttac tcatttgtag aatcaatata aatatattta tgacatactt cattcacatt
    12241 ctgttgtcaa caagtttatc actaataaat atattctcga tacgcaatta tacttcctaa
    12301 taaattatat tataaatatt ttacgatttt cgactcggac tatacaatag actgacatac
    12361 tattattaac ttaacattca aatatataca tccattaaca ttagcatagt cactatgttt
    12421 cattcaacaa attacattat cgaactatga aatagtcaga aattgctttt ggagtataaa
    12481 aaagcacttg tgcaaaaaca caagtgcttt aaacttaatt tattgttcac tagtttgaat
    12541 caagccatat ttaccgtctt tacggcggta aacgatactt gttccatcag tttctctgtc
    12601 tgtgaataca aagaagtcat gacctaatag attcatttgt aataccgctt cttctgaatc
    12661 cattggtttt aagctgaatt cttttgaacg aataatttct atctcgttat catcgtaagc
    12721 gtcattatca acttgtgttt cttgcatttc ttgtaattcg gcaacaaaca cttcttgatc
    12781 tcctcgatca cggctcttac gattaatacg tgttttatat tttcgaactt gtctttcaag
    12841 tttattatta attaaatcaa tacctgcgta taaatcatcg tttcgctctt cagctcttaa
    12901 cgtaacattt ttcaatggaa tggttacttc aattttagta gctgaatttg aataagtttt
    12961 aactttaaca tgcgccactg catttggtac gtcattaaaa taacgttcca acttaccaat
    13021 tttttcctca atatagttgc gaatagcatc tgtgatagtg aggttatctc catgaatttc
    13081 aaatctaatc atagtaatct ctccttaaac ctctttattg gtaactcttt attgtatttt
    13141 aacattttta cgctatcgtg caaacgcaaa cactttgaat tttctgatat ttttagcata
    13201 taatttacac cctgcacgat gaattgttaa tcctgttgta taaatatcgt caacgagtaa
    13261 tatttccttt ccatttaaat ctaattccgt atcaataata aatggatttt catctgccag
    13321 acgctctttc ttagttaaat gagactgttt tggtctattt gacatcttta aaatcttatc
    13381 aaagcggatc cctttagctt ttagtactgc ttctatcggg ttaaatgttc tagataaatc
    13441 atgtgccggc gaagaaggaa ttggcacaat atagtcataa gatgtttgtg gtatttcaat
    13501 caaatgtgcc
//
LOCUS       DAHHRR010000071        13463 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.071, whole genome shotgun sequence.
ACCESSION   DAHHRR010000071 DAHHRR010000000
VERSION     DAHHRR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13463
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.071"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(47..640)
                     /locus_tag="NBI42_001736"
     CDS             complement(47..640)
                     /locus_tag="NBI42_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD1778152.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            704..1087
                     /locus_tag="NBI42_001737"
     CDS             704..1087
                     /locus_tag="NBI42_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HCD1778153.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHERKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            1218..3623
                     /gene="mnhA1"
                     /locus_tag="NBI42_001738"
     CDS             1218..3623
                     /gene="mnhA1"
                     /locus_tag="NBI42_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HCD1778154.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWIWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTVMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTSSYVTDYSRNNLVIIFGALILLTFVTVFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            3616..4044
                     /gene="mnhB1"
                     /locus_tag="NBI42_001739"
     CDS             3616..4044
                     /gene="mnhB1"
                     /locus_tag="NBI42_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HCD1778155.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            4044..4385
                     /gene="mnhC1"
                     /locus_tag="NBI42_001740"
     CDS             4044..4385
                     /gene="mnhC1"
                     /locus_tag="NBI42_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HCD1778156.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            4378..5874
                     /gene="mnhD1"
                     /locus_tag="NBI42_001741"
     CDS             4378..5874
                     /gene="mnhD1"
                     /locus_tag="NBI42_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HCD1778157.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPMIKRYVALGGTLLTLV
                     AAIINLANVVKHGPLRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            5876..6355
                     /gene="mnhE1"
                     /locus_tag="NBI42_001742"
     CDS             5876..6355
                     /gene="mnhE1"
                     /locus_tag="NBI42_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HCD1778158.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            6355..6648
                     /gene="mnhF1"
                     /locus_tag="NBI42_001743"
     CDS             6355..6648
                     /gene="mnhF1"
                     /locus_tag="NBI42_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HCD1778159.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            6626..6982
                     /gene="mnhG1"
                     /locus_tag="NBI42_001744"
     CDS             6626..6982
                     /gene="mnhG1"
                     /locus_tag="NBI42_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HCD1778160.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            7246..8400
                     /locus_tag="NBI42_001745"
     CDS             7246..8400
                     /locus_tag="NBI42_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCD1778161.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFLDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKEHDQVVYDKTDHIVGSGITAAHLALKLLNHDDDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISDGLINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(8456..8830)
                     /locus_tag="NBI42_001746"
     CDS             complement(8456..8830)
                     /locus_tag="NBI42_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCD1778162.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMSVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHQGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(8849..10165)
                     /locus_tag="NBI42_001747"
     CDS             complement(8849..10165)
                     /locus_tag="NBI42_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HCD1778163.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(10576..12051)
                     /locus_tag="NBI42_001748"
     CDS             complement(10576..12051)
                     /locus_tag="NBI42_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HCD1778164.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPETFAEDIVNHFKNTKVKVEVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKYDMCGAANVVGIIEAASRLQLPLNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVYYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSKVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(12182..13390)
                     /locus_tag="NBI42_001749"
     CDS             complement(12182..13390)
                     /locus_tag="NBI42_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1778165.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
BASE COUNT         4250 a         2359 c         2114 g         4740 t
ORIGIN      
        1 agctttatcc gagtttaaaa tgttggtagt taattatgtt tagatattat tcttcaacat
       61 caatagattc aattacaaca tcatgaagtg gtttatcttg tggtcccact tttgtatttg
      121 caatatcttc taatgtagtt tcaccatcaa tgatttgacc gaatactgta tgtttttgat
      181 ctaaccatgg tgtaccaccc ttctcgccat atgcatcaac gattggttgt ggccaaccac
      241 catctgcaag ttgacttaac atattttgag gtacttcttt catttgaaca atgaaaaatt
      301 gtgaaccatt agtattaggt cctgagttag ccattgataa tgcgccatat aagttaaatg
      361 catttaatga aaattcatct tcaaaagcac cgccataaat actttcgcca cccataccag
      421 tagctgttgg atcgccacct tgaatcatga agtcattaat gacacggtgg aatgttattc
      481 catcataata accatttttt gcatgtgtca caaaattttc aactgttttt ggtgcaatat
      541 ttggaaataa tttgaatgtc atgtcacctt tatttgtgtg cataaccact ttaatttcac
      601 cttgttgtac ttctttgttt aactgtggat agttagccat ttatattctc cattcatgtt
      661 atgatacata tatattaaca tacttttaag ggaggaaaat agcatgaagc tatatcgatt
      721 ttcacacaaa acaggtagct atggtgtcac gattgttgaa gaaaatggag acgatgtact
      781 tgttaaagtt gaacaagtta tcaaacaccc gaaacaaggt gatttacata accctactga
      841 aactgaaggt gtatttttcc atgaacgcaa agcattaagt cattttgaaa aacgctatac
      901 gaaacgttca caattacgcg acttcaatgt tgatgtattg cgctatgaag attcacttca
      961 acaagcaatt acagatttag aaactaaact taaagctcag caaacaaaac atgctgaaat
     1021 gtctttagct agtttagcac gcttaaaaga agattattca cttcaataca aagaaaattt
     1081 taaataaaaa atgacgtgtt tcttcttatt taaatacttc tgaaaataaa gcacaacaag
     1141 gagtgtgtac gtttttcaga actatatcat tcatgtataa tgaataacat aaatattttt
     1201 taaaggagga caacatagtg agtttgttac atattgcggt gatattaccg ttaatattcg
     1261 cactcattat tcctatcctg tatagatttt ttaaacgtat acatttagga tggttcgtat
     1321 tacctgtccc tatcgtcata tttatttata tgcttacatt aattaaaacg acgatgtcag
     1381 gtaatactgt aatgaaaacc ttaaattgga tgcctcattt tggtatgaac tttgatttat
     1441 atcttgatgg cttaggttta ctctttagtt tgcttatttc aggaatcggc agtttagtag
     1501 tattatattc aatcggctac ttaagtaagt cagaacaact agggaatttt tattgttatt
     1561 tattactatt tatgggtgca atgttaggtg tcgtactttc agataatgtt attattctat
     1621 atttattctg ggagctaacg tcattttcta gttttttact tatttcattc tggcgcgaac
     1681 gacaagcatc tatatatggt gctcaaaaat cgctcataat cacagtattt ggtggtctat
     1741 cattattagg tggtatcatc ttactcgcta taccaacgca atcatttagc attcagtaca
     1801 tgattcaaca tgcttctgaa attcaaaata gcccattctt catttttgcg atgattttaa
     1861 tcatgattgg tgcttttaca aaatctgctc agtttccatt ttatatttgg ctaccagatg
     1921 caatggaagc acctacacca gtcagtgctt atcttcattc agcaacaatg gttaaagcag
     1981 ggttatattt aatcgctcga atgacaccaa tcttcgcagc atcgcaaggt tggatatgga
     2041 ctgtcacact cgttggttta attacgttat tctgggcttc gctaaacgca acaaaacaac
     2101 aagacttgaa aggtatactg gcattttcaa cagtatccca attaggtatg attatggcga
     2161 tgcttggaat cggtgccatt agttatcact atcaaggcga tgatagcaaa atatatgctg
     2221 ctgcatttac ggctgcaata ttccatttaa tcaaccatgc aacatttaaa ggtgccctat
     2281 ttatgattac aggtgcagtg gatcattcta ctggtacacg tgatgttaag aaattaggtg
     2341 gcttactaac agttatgcct atttcattta caatcactgt tattacagca ttaagtatgg
     2401 ctggtgtacc accgttcaat ggtttcttat caaaagaatc atttttagaa acaacattta
     2461 cagcaagcca agcaaattta ttcagtgttg atacattagg ctacctattc ccaatcattg
     2521 gtatagtggg cagtgtcttc acatttgtat attcaattaa atttataatg catatatttt
     2581 tcggacaata taaacctgaa caattaccta aaaaagcaca cgaagtatca atattaatgc
     2641 ttttatcacc tgctatttta gctacattag taattgtatt tggtttattc cctggcattt
     2701 taactaattc gattattgaa ccagctacat cttcgattaa tcatacggta attgatgatg
     2761 ttgaattcca tatgttccat ggattaacgc ctgccttttt atcaacatta gtgatttata
     2821 ttttaggtat cttactaatt gtgacattta gttattgggt gaaattgttg caacgacaac
     2881 ctggtaaact aacattcaat tattggtaca acagaagtgc aaatgttatt ccaaactact
     2941 ctgaaaagat gacgagtagc tatgttaccg attattcacg taataattta gttatcattt
     3001 ttggtgcatt aattttatta acatttgtca ctgtattctc agtacctttc aacattaatt
     3061 ttaaagacgt tagtccgatt agaattttcg aagtttgtat cgtaatttta ttattaagtg
     3121 ctgccttttt aattcttttc gctaaatcaa gattatttag catcattatg ttaagtgcag
     3181 ttggatatgc tgtgtctgta ttgtttatat tctttaaagc acccgactta gcattgacac
     3241 agtttgtcgt tgaatctatt tcaacagcat tgttcttatt atgcttctac cacttaccaa
     3301 atttaaatcg ttataatgaa aagcgctcat tccagcttac taatgcttta attgctggtg
     3361 gtgttggttt gtccgtcatt attatcggtt taattgcata cggaaataga cattttgaat
     3421 cgatatctaa gttttatcaa gagcacgttt atgatttagc tcacggtaaa aacatggtaa
     3481 acgttatact tgttgatttc cgtggtatgg atactttatt cgagtcatct gtgcttggta
     3541 tcgcaggatt agctgtatat acaatgatta aattacgtaa aaagcgtcaa acgcaaggta
     3601 acgaggtgaa aaatcatgaa tagacaacaa aatgatctaa ttttgcaatt tgctgcagtt
     3661 attatctttt tcatggtaat ggtctttggt ttttcacttt tcttagcagg acattataca
     3721 cctggtggcg gtttcgtcgg cggattatta ttcgcaagtt cacttgtaat cattacgatt
     3781 gcatttgata tagaaacaat gcgtaaaatt ttcccattag attttaaaat cttgataggt
     3841 atcggattag ttttttgtat tgcgacgcct atcgcaagtt ggtttttagg taaaaatttc
     3901 tttacgcatg tcacatttga tataccgtta tttatattag aacctgttca tatgacaaca
     3961 gcagtattct ttgatttcgg tgttttatgt gcagtagttg gtactgtcat gacaattatt
     4021 atttcgattg gagagaacga atagtggaaa ttattatgat ttttgttagt ggtattctca
     4081 cagcaattag tgtctatctc gttttgtcta aaagtctgat acgaattgtt atgggaacta
     4141 cactactaac acatgcagca aatttatttt taataactat gggtggactt aaacatggta
     4201 ctgttccaat ttatgaagcg aacgtaaaaa gctatgttga tcctatcccg caagcactta
     4261 ttttaacagc aatcgttatc gcctttgcga caacagcctt tttcttagta ttagcattta
     4321 gaacatataa agaattaggc acagataacg ttgagagtat gaaaggagtt ccagaagatg
     4381 attgaaagta atatgcttgt gttaacgctc gtcattccag taattacagc catattactt
     4441 gtctttattg gaaaacgtcc tatgataaag cgttatgtcg cactaggcgg tacattatta
     4501 accttggtcg cggcaattat caatttagca aatgttgtaa aacatggacc attacgagtt
     4561 gaactaggtt catggaaagc gccttacagt attgtctttg ttttagacat attcagtgcc
     4621 ctactaatta ttactagtat tattattaca gctattgtga ttttatattc ataccaaaca
     4681 attggtattg aacgtgaaag atattattat tatttttcag tactatttat gttaataggt
     4741 atcataggtg cattcacaac tggtgacatt ttcaacctat tcgtattttt cgaagtattt
     4801 ttaatgtcat cttatttctt attagtaatt ggctctacaa aaattcaact acaagaaaca
     4861 ataaaatatg tacttgttaa cgttgtatcg tcatccttct tcgtaatggg tgtagcgatt
     4921 ttatattcag tagtaggaac attgaatcta gcagatataa gcaataaatt agctaacctt
     4981 tcagcgcatg acagtggctt agtcaatatc gttttcatac tatttatctt tgtttttgct
     5041 acaaaagctg gcgtattccc aatgtttgtt tggttaccta gtgcatacta tgcaccacca
     5101 attccaatca ttgcattctt tggcgctttg ttaacaaaag ttggtgttta tgcaatcgct
     5161 agaacattga gcctattctt tagtgataat gtaagctttt cacattatgt cattttattc
     5221 ttagcactat taacgatcat ctttggttgt gtcggtgctg ttgcatatgc caacattaaa
     5281 aaaatcatcc tatacaatgt catgattgct gtaggtgtga tcttagtcgg cgttgcaatg
     5341 atgacagagt caggtatgat tggggctatt tactatacat tgcacgacat gttagtaaaa
     5401 cttgcattat tcttgcttat cggaattatg attaaaatta caggcactgc tgatttaaga
     5461 caatttggtg gtttaattaa acgatatcct gtacttggtt ggtcattctt tatcgcagca
     5521 ttaagtttag ctggcattcc cccactcagt ggcttttatg gaaaattttt cattgtccaa
     5581 tcaacatttg aaagaggatt ttatttaagt ggtgtcatcg tcttattatc aagtttagtc
     5641 gtattatatt ctgtcattcg catattccta caaggctttt tcggtcaacc aaaaggatat
     5701 gatcttaata acaaagtcga cgtcaaatat ttaactacga ttgcaatcgt tgcagttgtg
     5761 ataactgttt tatatggatt gtcagcagat tacttatatc caatggtaaa agctggtgca
     5821 gaaacattct acaatccatc aacctacgtt aaagctgtat taggaggtaa ataacatggc
     5881 tgtacaatta gtattaaact ttattatcgc agtgttttgg ttgtttgtga caaatagtta
     5941 cacaacaaat aactttgtgt taggtttcat ctttggccta gttcttgttt acctcttaca
     6001 cagagtgtta ccaggtagat tttatgtcat cacgctatat cgtattatta aattagttat
     6061 tattttctta atcgaactga ttaaagctaa ttttgatgtt ttgaaaatta ttattaaacc
     6121 ttcgattaaa aatgaaccag gattctttgt ttatcacaca gatttaaaaa aagattggca
     6181 aattgtcttg ctgtctaatt taattacatt aacacctggt actgtagtgc ttggtgtaag
     6241 tgacgatcgt acaaaaattt atattcatgc catagatttt tcaactaaag agcaagaagt
     6301 agaaagtatt aaaacatcat tagagaaaat tgtaagagag gtgggcgaaa tataatgaat
     6361 cataatgtta ttatcgttat tgcattaatc atagttgtca tttctatgtt agctatgctc
     6421 attcgcgttg tgctaggccc atcacttgcc gatcgtgttg tcgcattaga tgcgattggt
     6481 cttcaattaa tggcagttat agcattattc agtattttat taaatattaa atacatgatt
     6541 gtcgttatta tgatgattgg tatattagcc tttttaggta ctgcagtatt ctctaaattt
     6601 atggacaaag gtaaggtgat tgaacatgat caaaatcata ctgattagtc ttgcacttat
     6661 ctttgttatc atcggtgctt taattagcgc cctagcagcc ataggattat tgagacttga
     6721 agatgtatat tcacgtgcac atgctgccgg aaaagcatca acattaggtg caatgtcatt
     6781 actatttggt acgtttttat attttattgc tactcaaggt tttgtaaata tgcaattaat
     6841 cgttgcgatt atctttgtat taattacagg tcctctttca agtcatatga ttatgaaagc
     6901 agcatataat attaaaacgc cttatactaa aaagactaaa gtcgatgaaa tttctgaaga
     6961 cttaaaagac acaaaattat agattaaata acaaattcat gaatataaat cagcaacaaa
     7021 cagcagttag atgattccaa attaggaatg attttactgc tgttttcttt tgacattgtt
     7081 acctcttttt caatgatttt agtttagtac atacttcttt gactacagct tcgccctatc
     7141 cacgtatatc tctttaattt aattgccttt catgtcgtta tgtattatga taataataat
     7201 tataaatcgt aacgattacg ctttaaaaaa gagagaggtt ttattatgca ttggacaatt
     7261 atcggcggtg gcatacaggg aactgcaatc gcacaaaaac tattatcaag cggattaaca
     7321 acagaccgat taacaatcat tgacccacac gaaacttttt gccaaaggtt taactcatat
     7381 acaaatcgaa tagaaatgcc ttatttaaga tcaccgattg tacatcacgt acatccacaa
     7441 ccgttccatc taaaacaatt cgctaaacag caccaatata caaatgcttt ttatggtcct
     7501 tatcagcgac ctgaattgac aatgtttttg gatcatattg cacatgcttc taaacaatat
     7561 caattagagg attgtttggt tcaaggttta gttcaaactt tagataaaca agaagacaaa
     7621 tggcatatca agttagaaga tggacaaatt atcactacag attgcgtcgt tattgcaata
     7681 ggtagtacaa atattccgtt tatgcctgac attttaaaag acaaacagaa tgtaaatcat
     7741 atcttcgaga aagaacatga tcaagtagta tatgataaga ccgatcatat cgttggtagt
     7801 ggcattactg ctgcacatct tgcacttaaa ttgttaaatc atgatgacga taaaaagatt
     7861 catttatggc taaataaaga tattgaaata catgactttg atgctgatcc tggttggtta
     7921 ggtccgaaaa atatgtcttc atttttaagt actaaaagca tgcctgaaag aaatgccatt
     7981 gtacaacgtg aacgtcataa aggatcaatg cctcacgaac tgtacttacg ccttaaaaaa
     8041 catattaaaa atggccgtat aaatgtgcat aaaacaccta tcactcaaat tagtgatggt
     8101 ttaattaaca ctgaaaatga ttctgttcca tatcaacaga ttatggttgc aactggtttt
     8161 gaacaagatt ttatgtcaca accacttatt aagcaattaa tacaaaatta tgatgcacct
     8221 atcaacgaat gtaattaccc tgttatttcc gaaaaattag aatggatacc aaatctattt
     8281 gtcgcaggat gctttgcaga cttagaatta ggaccatttg gtagaaatgt tatgggtggc
     8341 cgtaaagctg ccgaacgcat tgaacaagca tttctaaaac tacaacaata tagtgcataa
     8401 aaaaactgct agccgtagtt atgaatatct cataactttt cagctagcag ttcttttatt
     8461 ttaaaggttt aatagcaact gtaccacgca taactgtaat taattgttct ttgtcattct
     8521 taatttttat atcccataca tgtgtcgact taccttgatg aataatttca gctgtcgcag
     8581 taacacgacc atctttagca gaatgaatat ggttggcatt catctctaaa cctaatggaa
     8641 taaatttggt tgtatcaatt agattagcag atcctaatga acatgctgtt tcacctaaag
     8701 caatcgaagc accaccatgt aaatatccaa atggttgttt tactttatca gtaacagaca
     8761 tagaaataac aactaaacca tcttcctggt gatctattga catctcaaat gtctctaata
     8821 aatgagtcat attttcacct ctttatgctt atagtaccat agcagcaata gtaccgaaaa
     8881 tcattaaagg aatattataa aacaagaagt ttggtacaca agtatcgcgt atatgatcat
     8941 gttggccatc aacatttaat cccgcagttg gtcctaatgt tgaatcactt gcaggtgaac
     9001 ctgagtcacc taatgcactc gctgttccaa tcaatgcgat taatgccata gtatctagtc
     9061 caattgacgc tccaaaagga atgaataacg atgcgataat aggaattgtt gcaaatgatg
     9121 atccaatacc taaagtgaca attaaaccta tcacatacat catgataatg ctaaataatt
     9181 tattatcacc agtaatactt gtcaaagttt taactaattc atctatatca ccagtagcat
     9241 tcattacacc agcaaatcca tttgctgtta aaataactac accaatataa gccataattt
     9301 taataccttc aacaaactta gcatctaatt cataccaatt atatgcacgt gaaataaaga
     9361 atacgagtac ccctgccagt gcaccaaaaa tcattgaatc tgtaaatgtt tgtactaaaa
     9421 atgtagctag tattgctaca attgttacta ttaagatata tggttttaac tctgtaacat
     9481 tgtcactatc tgaaatttta cgtgtttcat attcacgtgg tttacgatat acatataaac
     9541 cgataagtaa gccaacaata taccccattg aaggaataag cattgctttc caaatcatat
     9601 taaactcaat tgggtgattc gcctttgcaa agccactttg aataatttgt tggaaaattt
     9661 gaccgaatcc ataaggtaat aacacatacg ggaaacataa accaaaaccg ataatcaaac
     9721 cgattaaacg tctatctatt tttaagtcat taaacagact taataacggt ggaatgacaa
     9781 ttggaatgaa tgcaatatgt acaggaatta agttttgact catgatactc atagctaata
     9841 atgcaatgat tattgtcact tttactttaa ctcttgacca tcgactattt tcagcgtgaa
     9901 ttgcatttat aatttttcct actaaatagt ctgtgatacc actgtatgaa attaatgctg
     9961 caaatccacc taataaggca tagcttaatg ctacctcagc accatcgact atatttttcc
    10021 caaatacatt tataactttt tcaatgctca tgcctgaaat taagccacca actagcgcac
    10081 tgataaataa gcttataact acgtttaatc gacataaaca tagcacaatc attaatatta
    10141 ctgctattac tactgcattt atcatatgac aaccatcctt tatcactcta tcgaactaaa
    10201 gcgtttatag actacatagt atcagtagct ttaacacctg tcaatagcaa actatactaa
    10261 taatctttat tttttagtta attgtctttc ttaaaataat tttcgctttc attaaattaa
    10321 acaattttac aagcctggaa caccaatcaa aatcctaagt tctataatgt tatattagta
    10381 gtcgttgact gaatgaacat atgcttataa tacgtttttg caatgctagt caagttgatt
    10441 tatgctcaca aggatatgcg atttatattt tcttacaata atgaaaatgc ctgatacaat
    10501 gcgatccttt atactttcaa gagttaaatt aaaaatgtat aacaagacac aagttgtatc
    10561 aggcaattaa ttaaattatt gttgttttaa ccattgtact atcgtcggaa tcataaatcc
    10621 tgttggccct tttggaccat tatatgaagc tttattagtc gttgctggac ctgcaatatc
    10681 gaaatgaatg tgaggtgttt gaccactaaa atgtgttaca aaacttgccg caaatagcgc
    10741 tttaccttgt ccattcgtat ggttaactaa atcagcgata tcactgtgtt taatacttgc
    10801 acgttcggtt gcagtaatcg gtaattcaaa taccatttca tcgactttag aacttatttg
    10861 taatatatcg tttaatatca ctttactatt cgattcaaat gcagcagctt tatcatcgcc
    10921 tagtgcaaca attgctgcac ccgttaatgt agcaaagtcc ataatcacac taggctgata
    10981 ttgatttgca taatacacag catctgcaag gactaatctg ccttcagcgt ctgtattcat
    11041 tacttctaca gtttcaccac ttaatgctgt aaatacatca tctggcttca ttgacgcttc
    11101 atttatcata ttttcagcac aagcaagcac tccgacaata tttaaaggca gttgtaaacg
    11161 actagccgct tcaatgatac caacgacatt cgcagcgcca cacatgtcat atttcattgt
    11221 agccatgcca ttcttcgttt taatactata accaccagaa tcatacgtta tacctttacc
    11281 aactaaggca attggtgctt catctttgtc tttgccatta tatgtgatgg ttactaatct
    11341 cggtttatgc ttactacctt tacctaccgc ttgtaaaagt ccgaatcctt cagaaactaa
    11401 agtgtcataa tctttaacct ctactttgac ctttgtattt ttgaaatgat taactatatc
    11461 ttctgcaaat gtttctggtg ttaatacatt cggtggcata ttactaaagt ctcttgccaa
    11521 attaatggat tggccaatac taataccctc atgaataaaa tctaattcaa ttaatgattc
    11581 actaataagg tttaaattcg tcttaaacgg tgctttctta cttgatttat aatgatcgaa
    11641 ttcatatgtt gcacgctcac tttgaatgcc gcatgccatt aatacatctg ataactgatc
    11701 atattttgaa ataaatgaat ccataagtaa atacgtatct tcaatgtgtt ctgactttat
    11761 gtattggaaa agatgtcccc atattttcaa catatcttga taactacgtg tttttaagtt
    11821 tcctaaacca actgtaatta aacgatatgt ttgatcttgt acatcaaatg cagttgtata
    11881 aatcttccca actttactac caataatatg ttgatgtttt agtctttcaa gtgattctgt
    11941 aatatcgata tgattaaaac taatgcgctc taactgattt aaatgttctg gtataccaat
    12001 aatcaatgta tttatttcgt tgcttagtgt gttatttaat ttaaaattca taatgtacct
    12061 ccttagattt gatatgttac tcacttcaaa ttgtacaaca ataaagccct tcagtgacac
    12121 tgaaggactt tataaatgaa taatttaatt ttatgtgttt agctgtaact taatttaaag
    12181 cctagaattt accttttttg aatgctaaac cgataccacc gattttgaat accgcacgtg
    12241 tatcaatcac ttttttcatg aatgctgctt ttttaccagc gataggttta ccaaatacca
    12301 tacctacacc gtcatgtgaa cctaaagaac aaacagttcc acgatcaacg tattcgaatt
    12361 cttcagttga ttcaccgttt aagatgcgtt taatgttttt agcaacactt tcaccttgtt
    12421 gcattgcaat ttgtgctgta gttggtaatg gacgttcttc tccagctggg ataaacgctg
    12481 aacagtcacc aataacaaaa atgttgtcgt aaccattgat tgttaaatct tgctttgtaa
    12541 cgatacgtcc acgtttaacg ccttcaaatg attcttccat taatttacta ccacgtacac
    12601 cagctgccca tactgaagta cctgcattta attgttgttt ttcaccatct acttcaacta
    12661 cgaaaccttt ttcgttacaa gcaacgattg gtgtagcaat tttaaattca acaccgcggt
    12721 cttctaagta gctaactgcg tggttaacta attcttctga gaacattggt aacattttag
    12781 gtgctgcttc aacacaagtg attttaactt tattctgatc cacaccatat ttgctacata
    12841 attcaggaat tctgtctgtt aattcaccta agaattcaac accagtgaat ccagcaccac
    12901 caactaagat agataaatcg ttatcatctt tttcttttga tgctgcatag ttagcaaatt
    12961 tgtcttcgat atgacgtgat aattcacgtg ctgtgataac attttcaatt tggaaagcat
    13021 gatctttcat accttcgatg ccgaatgttt cactaacgaa acctaatgct actactaaaa
    13081 tatcaaagtc ataaatacct tgatttgttt ctaccttttt agcatcacgg tcaatttttg
    13141 ttacttctgc ttgaacaaag ttcactttgt ctttcttcaa gacactttcc acaggatata
    13201 atacatcttc atagtttagt gtacctgctg atgcttcatg taaccatgtt gcttcatagt
    13261 gatattcatt tttattaata agcgtaattt ctgcttcttc tgttgatatc gctttttgca
    13321 atttagttac agtttgtaaa cctgcataac cagcaccaag tacaagtact tttttacgat
    13381 cttgagccat ttaatttcac ctaagctttc atattttttt aaccaaatgc tgataattac
    13441 tagtaacaac tggtcattac tac
//
LOCUS       DAHHRR010000072        13251 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.072, whole genome shotgun sequence.
ACCESSION   DAHHRR010000072 DAHHRR010000000
VERSION     DAHHRR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13251
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.072"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(188..589)
                     /locus_tag="NBI42_001750"
     CDS             complement(188..589)
                     /locus_tag="NBI42_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1778166.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(1026..1238)
                     /locus_tag="NBI42_001751"
     CDS             complement(1026..1238)
                     /locus_tag="NBI42_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778167.1"
                     /translation="MNDKTSKDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQGQI"
     gene            complement(1268..1879)
                     /locus_tag="NBI42_001752"
     CDS             complement(1268..1879)
                     /locus_tag="NBI42_001752"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCD1778168.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKQAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            complement(1879..2571)
                     /gene="pyrF"
                     /locus_tag="NBI42_001753"
     CDS             complement(1879..2571)
                     /gene="pyrF"
                     /locus_tag="NBI42_001753"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCD1778169.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLSTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            complement(2681..5854)
                     /gene="carB"
                     /locus_tag="NBI42_001754"
     CDS             complement(2681..5854)
                     /gene="carB"
                     /locus_tag="NBI42_001754"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCD1778170.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFELDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENEIKPVYKMVDTCAAEFESSTPYYYGTYETENESIITDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            complement(5847..6947)
                     /gene="carA"
                     /locus_tag="NBI42_001755"
     CDS             complement(5847..6947)
                     /gene="carA"
                     /locus_tag="NBI42_001755"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCD1778171.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLDTGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKKLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            complement(6949..8223)
                     /locus_tag="NBI42_001756"
     CDS             complement(6949..8223)
                     /locus_tag="NBI42_001756"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCD1778172.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSAGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKVIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLVGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            complement(8241..9122)
                     /locus_tag="NBI42_001757"
     CDS             complement(8241..9122)
                     /locus_tag="NBI42_001757"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCD1778173.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            complement(9150..10457)
                     /locus_tag="NBI42_001758"
     CDS             complement(9150..10457)
                     /locus_tag="NBI42_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCD1778174.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIAVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            complement(10675..11202)
                     /gene="pyrR"
                     /locus_tag="NBI42_001759"
     CDS             complement(10675..11202)
                     /gene="pyrR"
                     /locus_tag="NBI42_001759"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCD1778175.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            complement(11603..12520)
                     /locus_tag="NBI42_001760"
     CDS             complement(11603..12520)
                     /locus_tag="NBI42_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD1778176.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNSDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTGEYIERHAELPQDFEDLLDTIRKRDA"
     gene            complement(12520..13011)
                     /gene="lspA"
                     /locus_tag="NBI42_001761"
     CDS             complement(12520..13011)
                     /gene="lspA"
                     /locus_tag="NBI42_001761"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCD1778177.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRVLTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
BASE COUNT         4074 a         2524 c         1921 g         4732 t
ORIGIN      
        1 gcgcaattat ctctataatt aaaagtgcct aaacaattaa ctaaatgaga taatgcgcct
       61 atgaacaatg ttatactata ttcctttaat ttgaaaaata aaaatattat tttttacgat
      121 aaacttgaaa aaattgagta taaagggaga aagtgtttct ttatcattca acgctctgcg
      181 aatcctttta ctcaggtaat gctaattgaa aacttactcc aaacttatct tgaacccaag
      241 caaactctct gtatgggggc atattcgttt ttggcattaa aatggcacct tcatctttta
      301 atccattaaa tagtcgttcc atttcaatag tatcttttac tgtaacaaat aatgaaatcg
      361 caggattcat tggtaattct gtgccactat tagcatcaat cgccatgaat acttgtccat
      421 ttaatgtaaa tattgagtgt tgtacagtcc caggatcacc aggtccattt tcaccatact
      481 tagccattgt tataatctca ctatcttcaa ataagcttgt gtatagttta acagcttctt
      541 cagcttggtt attaaacatt aaaaatgtcg taatttttgg aatatccatg ctatgcctcc
      601 tatagattga acttctataa ctactattat cttaacataa ttaaaatgac aaaagcgcgt
      661 tgtaccgtta tgtgcacgaa tgacaaatgt cttattggcc tgttgattaa atcacatagt
      721 taacgataaa aattacgcaa gattaaaaat atgatacata tgttaataaa tgatgcttat
      781 cataggtatt aatgttttga cttattgtca aatttgatta caattaaaat taagttaaag
      841 gagggacgtt atgttacatt atgtgattcc agtattagtc ctattactcg tattcttaat
      901 tggttggatt ttaataaagt taattatccg ctaataaatt ttttatataa taaaagctat
      961 agtgtttgta atattctgat gcggttaagc agcccctgat attaaacact atagcttttt
     1021 tgtttttaaa tttgtccttg tttccgtaat atatccttta catgtttaaa taaatcatca
     1081 ttatgatcta tagttgtttt attttttata tgtttttgct tcgttgaact ttcatcgaca
     1141 tcgtgactac tagatagatt ggaatcattt gaatgattga taaattcgtt acaatgtttt
     1201 atcttcccat ataaatcttt agatgttttg tcattcattt gttgaattcc tccttctagt
     1261 gtctatatta tgctaagttg tctctccatt caactaatgt ttggatatct tcactagaaa
     1321 ttttaccttc attttcagct acttcaatta attcattgta atcgcttaaa gtgtaaaaag
     1381 gtagttgaat attgctaaat gtatcatctg cttttttcaa accgtaagta aagatagcta
     1441 caacacctaa tacttctgca cctgcttgtt ttaaggcttc aactgctgtg actgaagaac
     1501 cacctgttga aattaaatct tctatcacaa cgactttttt accttcgctt ttagcacctt
     1561 cgatttgatt ttgcttacca tgactcttat ttgatgaacg aacataattc attggtaatt
     1621 ttaatttttc agcaataaaa gctgcatgtg gaataccagc tgttgcagta ccagaaataa
     1681 cttctacttc aggaaagtgt tctttaatta agttaattaa accatcgcgg attgcgcctc
     1741 gaactaaagg ataacctaac gtaacacggt tatcacagta aatcggtgat ttaatacctg
     1801 aactccatgt atataaatca tttggtgata atgttacagc ttcaatatct aataatgatt
     1861 ttgcaatttc tttagccatt aaactaacca actttcttta attttatgat aactttcgac
     1921 tggattgtca ctttgtgtaa tcggtctacc gactacaata tgcgtcgaac caagctgtct
     1981 tgcttcttcc ggtgtcgtaa tacggtgttg gtcatcttga gatgcacctt taggtctaat
     2041 acctggtgtt acttttaaaa atgatgtact taacttttca gtcaacatac gactttcaag
     2101 aggtgaacaa acaacgccat ctaaaccagc tgcatttgct aacttggcat aatttaaaac
     2161 ggcctcttca atcgatgttt gtatattttg ttcgtgtcgt aacatgtctt ctgttgttga
     2221 cgtaagctgt gttactgcaa taatttttgt atgttgatta tgttttctta atcctttaat
     2281 ggcctcagac atcattttta cgccaccagc agcatgaaca tttaccagat caacattcaa
     2341 tttagctagt ccttccatcg ccttaccaac tgtattggga atatcatgca gttttaaatc
     2401 taaaaataca tcatggcctc tttcttttat ctcattaatt aattgaggac cttcttgata
     2461 aaaaagctcc atacctactt ttacgaataa tgattcatca aataaatcta aaaattgatt
     2521 tactttttct tttgattcaa aatctaatgc aataattggt aaatctttca tcatatatgt
     2581 ctccctttac gttaagaaat tcattaaaat aatgaggctg taatataaac ttctttaagt
     2641 aaatggttaa ttacagcccc accgatacag tttgattgat ttacatttga cgcattgtaa
     2701 atgtcatact ttcaattaca ttcgttaagg cattagctgt atctaaagat gtcaaacatg
     2761 gaataccatt ttcaactgta gtacgtctaa tttggaagcc atctctttct acttctttac
     2821 ctttagtcat tgtatttata acgatttgaa catcaccatt ttgaatacgt gttaataaat
     2881 cattttcgcc accaattttg cctactactt ctgcaggtat gtcatactca gctaatttat
     2941 tagctgttcc agacgttgct aaaattttat agccaacttc atttaagcgt tgtgccaatt
     3001 taacaacttc ctctttatct ttgtcactga cagtcattaa tactgtaccg tgatctttaa
     3061 cttcaacacc actacctgtt aaccctttga ataacgcctt ttctaatgta gtatctttcc
     3121 ccatcacttc acctgtagac ttcatttcag gtcctaaagt aatatcaaca tttttcaatt
     3181 tattaaaact aaataccggt gctttcacaa atacaccctc agcatatggt tgaacccctt
     3241 cttgataacc catatctgtt agtttttccc caatgattgc tcgcatagct aattgtgcca
     3301 ttggaatatc agtaatttta cttaagaatg gtaccgttct actagaacgt ggatttactt
     3361 ctaaaacata cacaccatcg tgagctataa cgaattgaat gttgattaag ccaatgatgt
     3421 ttaaaccttt agctaatttt atagtatagt cctcaagtgt tgctaactcg tcttctgtca
     3481 aagtttgtgg cggatataca gcgattgagt caccactatg cacaccagca cgttcaatat
     3541 gttccatgat tcctggaata atgaccgttt ctccatcaca aatcgcatca acttcaattt
     3601 ctttaccagt taaatatcta tcgactagta ccggatgttc cggactcgct tttacagcct
     3661 gggtcatata gttttctaac tctttgtcat tgtctacaat ttccattgcg cgaccaccta
     3721 atacataaga aggtctgact acaaccggat atccgatttc tgcagcattc gctaatgctt
     3781 cctcaggtga tgtagctgtt ttcccttgtg gctgtggcac gttaattttt cttaatagtg
     3841 cttcaaattc ttttctatct tcagcacgat ttagattttc tagtgaagta ccaagtattt
     3901 taacaccatg tttagccaat ttgtctgcta aattaatcgc tgtttgtcct ccaaattgta
     3961 caacgacacc tttaggtttt tctaaattaa tgatattcat cacatcttct tcagttaaag
     4021 gttcaaagta taatttgtca gaaattgaga agtctgttga tactgtttct ggattgttat
     4081 tcacaattat cgcttcgtac cctgcttttt gaattgccca aacggcgtga actgtcgcat
     4141 agtcaaattc tacaccttga ccgattcgaa ttggtccaga gcctaatact aagatttttt
     4201 ctttgtcagt aattatggat tcattttcag tttcgtatgt accataataa tatggtgttg
     4261 aagattcaaa ttcagctgcg caagtatcaa ccatcttata aacaggttta atttcatttt
     4321 ccatacgcaa ttgatatact tcttcttccg tcatattaaa gcgatgcgct attgttttat
     4381 cactaaatcc ataatctttt gcatatttaa gatattctaa atcaccttga tgttctttta
     4441 gttgatgctc aatatcaata atgttttgga acttgtgtaa gaagaagtaa tcaatctgag
     4501 tcatattatg aatttcttct aatgttgtgc ctcttctaat tgcttcgccg atgaaaaata
     4561 atcgttcatc atcttggtgt gaaatacgtt ctttaatata atcaagttcg aagctttcac
     4621 catttggtaa tcctaagtga tgcacaccat actcaagtga tcgaattgct tttaacaatg
     4681 attcttcgta agttcgacca atggccataa cttcacctgt tgctttcatt tgtgtgccaa
     4741 gctctcgttc tcctttttca aatttatcaa aaggaaatct tggtattttt gaaatcacat
     4801 agtctaaagt tggttcaaac gctgcataag atgttcctgt aattggattt aacatttcat
     4861 ctaatgttag accaaccgcg attttagcag ctaattttgc aataggatat cctgttgctt
     4921 ttgaagctaa cgctgatgaa cgtgatacac gcggatttac ttctataata taataatcga
     4981 atgaatgcgg atctaatgct aattgaacat tacaaccacc ttcgatacct aaagctcgaa
     5041 taacttttaa tgaaacatca cgtaacattt gatactcaac atctgataat gtttggctag
     5101 gagccacaac aattgaatcg cctgtatgaa taccaactgg atcaatattt tccatgttac
     5161 atacaacgat ggcattatcg tttttatcac gcattacttc gtattcgatt tctttaaaac
     5221 ctgcgataga tttttctaat aaacattgcg ttgctggact ataatgaaga ccatttgaga
     5281 cgatttcatg taattcttca tcattatgac aaataccgcc tccggtacca cccatcgtaa
     5341 atgctggtct aacaattagc gggtatccca cttgctcttt gaatttaaag gcttgctcta
     5401 ctgtatttac aatgtcactc tctggtacag gaacgtttaa atcattcatt aatgttcgaa
     5461 acatttcacg gtcttctgct tgttgaattg atgttagctc agttcctaat aattggacgt
     5521 tattatcttg aagcacacca ctttcgtgta gttgaatcgc catgtttaaa cctgtttgac
     5581 cacctaaagt tggaagtaaa gcgtcaggtt gctctttacg tataattcgc gctataaaat
     5641 catgagttaa cggttcgata tatactttat ccgcaatttc cttatcagtc atgattgtcg
     5701 ctggatttga atttacaaga ataactcggt atccctcttc ttttaaagct agacatgctt
     5761 gtgttccagc ataatcaaat tcagctgctt gacctatgat aattggtcca gaccctatta
     5821 ctaaaattgt tttgatatca ttacgtttag gcattgatat gacgctcctt ttccttaaaa
     5881 ttagtcatca ttgctacaaa atcatcaaat agatagtttg aatctgacgg tcctggattt
     5941 gcttcaggat ggtattgaac agaaaatgct ggtaattttt tatgttttaa accttctaca
     6001 gtaccatcat ttaatgcaag atgagtaact tctaaatcag tactttttaa cgaatctata
     6061 tctattgcat atccatggtt ttgactcgta atatcaactt ttccagtatc taaattttta
     6121 actggatggt tcgcaccacg atgaccaaac ttcattttaa atgagcttgc tccttgagat
     6181 aatgcaaaga gttgatgtcc tagacagata ccaaagaacg gaattttccc taaaattcct
     6241 tgaatcattg gaatcgcaca ttctacaact tcaggattac ctggtccgtt tgatagcata
     6301 acgccatctg gagccattgc taaaatttct tcagcagtag ttgtgtatgg tacaactgtg
     6361 acgttacaac ctctgacgtt taattctcga acaatatttt gcttcttacc aaagtctaca
     6421 agtacgacac ttagatcctt acctgtcgaa acatacggtg ttttagtcga aacgatttct
     6481 acttcgtttt taggtaattc tacttgttgt aaatgtttga caagttgatc aatatcttct
     6541 tttcgatcag taaaaccagc ttttaacaca ccgtgttgtc gaattttacg cgtaatactt
     6601 cttgtatcaa cacctgcaat ccctggaatt tgatgcaatt ctaaaacgtc atgaagtgtc
     6661 ttttgttgtc taaaattact tggatgcgca ctcgcttctt tcactacaat accgtttaat
     6721 gtaggtacta atgattcaaa atcgtctcta ttgataccat aattaccgat taatggatac
     6781 gtaaatgtaa tgatctgacc tgtatatgat ggatctgaaa tagtttcttg ataacctgtc
     6841 atcgctgtat taaatacaat ttctcctaca gttaagttat cagatcctaa acggtagcct
     6901 tcgtaaaaag aaccgtcttc taacactaga taacgtttgc tttgcatatt atttatcccc
     6961 ctcaaattta acttcgcctt caaccattgt taagatcgga tttccataaa ctttatagcc
     7021 gataaatggt gtattatctg ctttagataa gaaatcttca cctttaattt cttgttcact
     7081 atctaaatca atgattgtta aatctgcata accattttct tttaatgtgc cgtattctaa
     7141 attaaatgtc tcacatggtt taattgttaa gtaatctact aattgttgta atgtccaatc
     7201 accatttttt acaaaatgcg tatataataa tgggaatgct gtttcactac caacaattcc
     7261 gaatggtgct ttttccattg gttgtgcttt ttcatcacgt gcatgtggtg catggtctgt
     7321 tgcgatacag tcaattgtac cgtctagtaa ccctactaac aaagcctctc tatcttcagt
     7381 acttctcaat ggtggattca ttttataaat ggcattgtta ccaggaatat catcttctgt
     7441 taacaataaa tggtgtggtg taacttcagc tgtaacatga atgcctgcgc gtttagcatc
     7501 acgaatgact ctaacacttt ctttagtaga aacatgacat acatgataat gacaacctgc
     7561 tgcttcagct aatagtacat ctcttgcgat ttgaacagat tcacaaatgt ttggaatacc
     7621 tggtatacct aactctttac tgcgtttccc ttcatgcatt gcaccaccat agattaatga
     7681 attatcttca cagtgtgcta cgatgacttt gtttactttt gcagcttcaa tcatgccttc
     7741 atacatcatg cttgcagttt gtactcctac accgtcatct gtaaacgcaa aggcaccttc
     7801 ttttactagt gctgggaaat caaccaattc tttacctaat tgacgtgttg taattgaagc
     7861 ataaggtaat acacgtactt gagcattgtc atcgattaat ttttgtaaag cttcaaaatg
     7921 ttctacagaa tctggtaccg gtcttgtgtt aggcattgga catacagttg taaatccgcc
     7981 tctagcagca gctttagtac cagtttcaat tgtctcttta tattcaccac caggttcgcg
     8041 taaatgaaca tgaacatcga caaatccagc tgacacaaag tgacctttcg catctatgat
     8101 gtcaacacca ttgcttggtt caattgcagg tgcaatttgt ttaattacct taccatcaat
     8161 taaaatatct gcttgttgta attcgccatt ttgtaatact ttaccgtttt taattaattt
     8221 catcattttc gtccccttac ctattttaat aattcatcaa tgactgccat tcttaagtaa
     8281 acgccatttt ccatttgctt aaaaattctt gattttgaag cttctactaa atcgctttgt
     8341 atttctactc ctctattcac aggtgccgga tgcattacaa tagcatgttc ttgtaatttg
     8401 ttatagcgca cttcatttaa gccatgcttt tgatgataat catctgctgc aaatctagtt
     8461 tcttctgcaa gcccatgtct ttcatgttga attcttaata acataactat atctactgtt
     8521 tctataacat catctatatt tacataaggt gcttctaaag aatcatcaat ccaagcattt
     8581 gggctattaa acattacgtt tgcacctaat gcttttaaac tatggtaatt actacgtgcg
     8641 acacgtgaat ttttaatgtc gccacaaatc aatacattca agccttcaaa atatccatac
     8701 tcttcatata tcgtcattaa atcaagcaaa ctttgtgttg gatgttgtcc actaccatca
     8761 ccagcattcg caattgggat gttaatattc gctaattttt catagtagtt attaaacgga
     8821 tgtctaatga ctaataaatc acagccaata ctttctaaag ttttacatgt gtcatataaa
     8881 gattcacctt ttgaaacaga tgatgttgat gtttcaaagc taatcgtttt taaccctagc
     8941 ttaagttctg ccatttcaaa actacatttt gttcgagtag aattttcaaa gaataaattt
     9001 gcgacatatt tcccttcaaa gtttggtagt tgacgttcac cagatttaaa ttgacttgct
     9061 ttttggataa gtttgtatat ttgatctgta gataaatgtt ccattgataa taaatgattc
     9121 ataaaagcgc cctccttaat ttgtaaatct taattgtttt gttttttctc tttaggtaag
     9181 attaagttca aaataattcc tgaaagtgca gctaatgcca tcccctcaat ttgaaggttg
     9241 ataccaattt cttttaagtt aaatactaaa ttaccgatac ctacaactaa aattactgaa
     9301 gctataacta aatttcgatt gttcgcaaaa tctactttgc tttcaactaa cattcttaag
     9361 ccacttgctg caataatacc gaaaagtaat atagatactc ctcccataac tggtgtaggt
     9421 atagaagaaa ttaatgcagt gaacttacca atgaatgcta aaacaattgc tataactgct
     9481 gcaccaccaa tgacgtaaat actgtatatt ctggtaatcg ctaatacacc gatattttca
     9541 ccgtatgttg tacttggtgg tccaccaata atactggcaa acattgtaga aacaccatca
     9601 ccaatgattg atttatcaag tcctggcttt tcaaagaagt ttctacctac gattttattc
     9661 aataccattt ggtgcccaat atgttcactt actgttacaa acacaatcgg aatcattaca
     9721 agtacaagtc ctaagtgaaa tgaaggtaca taatctttaa atggcagata aatatgaggg
     9781 aaatctatcc attttgctga cataattgca tcaaatttca caatccccat aaaaatcgct
     9841 accacataac ctacaataat acctacaagt actggaatta aagataggaa tcctttaaaa
     9901 aatccttgaa cggctattgt tacaagcaag gttatcattg caacaattaa gaaactgata
     9961 ttgtaacctt tcatatctcc aggattttca tacattgcca tattgactgc agtaggcgct
    10021 aagcttaaac caatgaccat gatgactggt cctacaacaa ctggtggtaa taatttcatt
    10081 aaccatgctg tcccacttaa tttgattaga atcccaatga tgacgtacat aacaccactc
    10141 atgaataatg ctacaagcat gtctcctaag ctatgcgtac ttaatcccgt gataattggt
    10201 gtgataaatg caaagctaga tcccaagtat gctggtattt gcgccttcgt tattaagata
    10261 taaagtaatg taccgattcc cgaagctagt aacgctgcgg atattggtaa tcctgttaag
    10321 aatggtacta gtactgttgc gccaaacatc gcaaataaat gttgtaagct taaaaatgcc
    10381 cattgcgctg gttgtggttt ttcatttaca tctagtacag gttttactgt tcgttcaaac
    10441 atttcatcat tttgcataat attcatttcc tccgataata aaaaaatctc tttacatcag
    10501 tatatgtaaa gagacaaaaa gtgtgacaag ttgctacaag tcattttcgt ccatagaaat
    10561 tgacttatag ttgtcgaaca tgagggtatt attagataaa caagcatatg aaaacttatt
    10621 tatcattcaa ctcccccacc tttttcagtc tctcgtactg aattaaaagg ggtattattt
    10681 aattataact gcatttcttt gatccatttc ttctaaatag acacttaccg tttcctcttt
    10741 agaagtaggt atatttttac caacaaaatc tgctcgaatt ggtaactcac gatgtcctcg
    10801 atcaaccaag gcagctaaac caattttaat aggtctagca tttagcaaaa tagcatcaag
    10861 tgaagcacga accgttcgac cagtatacag cacatcgtca ataatgatga ctactttatc
    10921 tgtaatatct gtgtcgatgt ctattgcgtc ttttgtcgta agtgatgaca tgtgctctat
    10981 atcatctcta aagtatgtaa tatcaattgt tcctgtaggt atacgttgtt gctcaatttg
    11041 atgaatttta tcttgtatac gattcgctaa atattcacct cttgttttga tacctaaaag
    11101 aattaaatta tcagtacctt tgttatactc caaaatttca tgagcgattc tcgttactgt
    11161 acgttgtatt gcggcatcat ccattatgat acgttcagac atattctcac cctttcaaaa
    11221 tttagaaaca aaaaacccca tgtctttaat atcgacatga ggtttagtat atacaatcag
    11281 cctatttaca tagaagaatc gtagtattac tacttattct tgcatatgtg aaagtgttat
    11341 gattcatttt agtatataac tatattttca tgtctttgac gtctctctgg acgctcaatt
    11401 aaagactctt tatgttgtac ttaaactgtt ataaggataa tactaatcag acgctttgtc
    11461 aatctatatt gaaaaattaa ccattctcag taaaaatgtt aaccttaatc tattttataa
    11521 tgtgttttca tttttaaaaa acgaaaacga taaatactta tggtttacat aataatgtta
    11581 tcgtaattat agcacgacac aattatgcat ctctttttcg aattgtatct aataaatctt
    11641 caaagtcttg tggtaattca gcatgtcttt caatatattc acctgttact ggatgttcga
    11701 atccaataag tccagcatgt agagcttgac caccaatatc caatgtcttt ttcggtccat
    11761 actttggatc accaactaat gggaagccaa tatatttcat gtgcacacgg atttgatgcg
    11821 tacgtcctgt ttcaagttga cattcaacaa gcgtataatc tttaaaatgt tctagtacgt
    11881 taaaatgtgt cactgcttcc ttaccatcat caacaacagc catagattga cgatcatttt
    11941 tgtttctacc aattggcgca tcgattgtac cgtaatcatg aggaatattc ccgtgaacta
    12001 aagcgatata ttttctttta acagatttat ccattaattg ttctacaagc ccacgatgag
    12061 caatatcatt tttagcaacc attaataaac cagaagtatc catatctata cggtgaacaa
    12121 tacctggacg aatttctcca ttaatacccg acaaattttt aatttgatac attaaaccat
    12181 taactaatgt attggtataa tgccctggtg atggatgaac taccatgcct ttcggtttat
    12241 atacaactgc aacatcgtca tcttcataat aaatatctaa atttaaattt tcaggtagaa
    12301 tatcagcttc aaccacttct ttttcagtga caactatatg atcattaagt ttcactttat
    12361 aattagattt aacaacttta tcgtttgcaa cgactaaacc tgctttaatc caatcttgta
    12421 tctggttacg agaccaatca ctatttaatt caggcagcaa cttatctaca cgcatacctg
    12481 tttgttcttt atctgtaatg ttaaattcat aagtctccat tacttaacct ccttctcctt
    12541 tttattggaa gtatccttta gtaaggcaat aataattaat attacaccaa ttgttaaact
    12601 tgaatctgcg atattaaata ttggaaaatc ataaccaaaa atatttgtat caataaagtc
    12661 aacaacttct cctgttaaaa ctctatcaat aaagtttcca agtgcacctg caaaaagtaa
    12721 actaatagca acttgcataa acaaattata ttgagcatct ttaataaaga aatatactaa
    12781 ggctattaat ataataatgg taataataaa gaaaaatgtc atttttccac tcaatattcc
    12841 ccatgcagca ccattatttc gatgtgatgt tatgtttaaa aagtgcggta tcacttcaaa
    12901 tgaatctcca attttcattg tagtagctat aatatattta gtgacttggt caaatataac
    12961 gacaaatact gctattaaaa tggaagtgcc aataaaatat tttttgtgca ttttcgttcc
    13021 tccaatcaat cgtccataag acaactcttt atattatagc ttacacctgc taataaaaaa
    13081 agtaagcata ttacattaaa tctaatgtta ctaactcaat acttgataaa ctactatgtt
    13141 ttgacattaa atatgaactt aattattcat ttatcatatt taagatgaca ttaaaaatta
    13201 ggaaacaggc tgggacataa atccctaaaa aaacagcagt aagatatttt c
//
LOCUS       DAHHRR010000073        12697 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.073, whole genome shotgun sequence.
ACCESSION   DAHHRR010000073 DAHHRR010000000
VERSION     DAHHRR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12697
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.073"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..558
                     /locus_tag="NBI42_001762"
     CDS             <1..558
                     /locus_tag="NBI42_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1778178.1"
                     /translation="EKVLIHVSNFRQVKRIDTIIETFAKVREKIPSKLILLGDGPELV
                     PMRQMTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLLSEKESFGLTLLEAMKTGVVPI
                     GSNAGGIKEVIKHGETGFVVDVGDCDSASAYAIRLLEDKVLYNKLQKNMLADIAERFG
                     SELITDQYEYYYQKMLNEHNKSKGE"
     gene            563..1765
                     /locus_tag="NBI42_001763"
     CDS             563..1765
                     /locus_tag="NBI42_001763"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCD1778179.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRTQMADIKFLSIERIVIELTKLMRGNNVEESFNHLKS
                     LKAFNYMPYFEHLDINQINVTEPIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSVITKEQLKMFVYDYDTNLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAYLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            1752..2723
                     /locus_tag="NBI42_001764"
     CDS             1752..2723
                     /locus_tag="NBI42_001764"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCD1778180.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     DFDESIRHRATSIQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            2747..5440
                     /locus_tag="NBI42_001765"
     CDS             2747..5440
                     /locus_tag="NBI42_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCD1778181.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVNQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFDAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKQ"
     gene            5761..7053
                     /gene="asnS"
                     /locus_tag="NBI42_001766"
     CDS             5761..7053
                     /gene="asnS"
                     /locus_tag="NBI42_001766"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCD1778182.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            7382..8068
                     /locus_tag="NBI42_001767"
     CDS             7382..8068
                     /locus_tag="NBI42_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCD1778183.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKEQNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDSAIIQ
                     AALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            8058..8717
                     /gene="nth"
                     /locus_tag="NBI42_001768"
     CDS             8058..8717
                     /gene="nth"
                     /locus_tag="NBI42_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCD1778184.1"
                     /translation="MVSKKKALEMIGVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            8722..9063
                     /locus_tag="NBI42_001769"
     CDS             8722..9063
                     /locus_tag="NBI42_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778185.1"
                     /translation="MTNITKEVFDNLEREIDVFAKNKTLGSNEAKPYLDEYHSKIIDY
                     FKQVNDITSNIDFDNLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(9580..11763)
                     /locus_tag="NBI42_001770"
     CDS             complement(9580..11763)
                     /locus_tag="NBI42_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD1778186.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDVKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(11760..12437)
                     /gene="recU"
                     /locus_tag="NBI42_001771"
     CDS             complement(11760..12437)
                     /gene="recU"
                     /locus_tag="NBI42_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCD1778187.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNAFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
BASE COUNT         4543 a         1809 c         2214 g         4131 t
ORIGIN      
        1 gaaaaagtgt taatacatgt atcgaatttt agacaagtaa aacgcataga tactattata
       61 gaaacatttg caaaagtacg tgaaaagata cctagtaaat taattttact tggtgatggt
      121 cctgaattag tgccaatgcg tcaaatgacg aaagagttaa acgtagaaga ggatgtttta
      181 tttttaggga aacaagattg tgtaagtgaa ttttaccaat tatctgactt agtattatta
      241 ttaagtgaaa aagaaagctt tggactaact ttacttgagg ctatgaaaac aggcgtcgtt
      301 ccaattggtt caaatgccgg tggaattaaa gaggttatta aacatggtga aactggattt
      361 gtcgtagatg tgggtgattg tgattcagcg agtgcctatg ctatccgatt acttgaagat
      421 aaggtgttat acaacaaact tcagaaaaac atgctagcag atattgctga acgttttgga
      481 tcagaactca ttacagatca atatgagtat tattatcaaa aaatgctaaa tgagcataac
      541 aaaagtaaag gcgagtaata atatggataa atcattattt gaacaggcaa ggcctatatt
      601 agaacaaatt caagacaatg gttttgaagc atattatgta ggtggctctg taagagatta
      661 tgtcatggga agaaatattc atgatataga tatcacaaca agtgcaacgc cagatgaaat
      721 agaatctatc tttagtcata ctatacctgt aggtaaagaa catggcacca taaatgtagt
      781 ttttaatgat gaaaattatg aagtgacaac attccgtgct gaagaagatt atgtcgatca
      841 ccgtagacca agtggtgtta catttgttcg tgatttatac gaagatttgc aacgaagaga
      901 tttcacgatg aatgcgatag caatggatac agcatacaaa ttgtatgatt attttgatgg
      961 tcaacaagat attaataatc gaataataag aactgtaggt atagctgagg aacgattcca
     1021 agaagatgct ttacgcatga ttcgatgttt aagattccag tcacaattat catttgatat
     1081 tgcaacggaa acatttgaag cgatgcgtac acaaatggca gatattaaat ttttatcaat
     1141 tgagcgtata gtgattgaac taactaaatt aatgcgaggt aataatgttg aagagagttt
     1201 taatcattta aaatcgctga aagcatttaa ttatatgcca tatttcgaac atcttgatat
     1261 aaatcaaatt aatgtgactg aaccaattga tttagaattg ttgattgcta tagtatcagt
     1321 taaatttgat attaattact cattgaagcc tttaaaacta agtaaccgac aagtaaaaga
     1381 tatcaatcaa tatattcaaa ttatgaatgc attaccaagt gttattacaa aagaacaatt
     1441 aaaaatgttt gtttatgatt atgatacgaa tctcattaaa aatgtaatgg ttgcagcaga
     1501 cgtgttaaag gcaaatgata ttcaaggaca tgaaccatta atcgttaacc ttcaaacgat
     1561 tgatgaaaca ttacatcgtt tacctatgca taatagaaaa gacatgatgg ttaatggcgg
     1621 tgtacttatg gcatatttaa atgccaaaag tggtccgtgg ttaaaagatg tgctaagaca
     1681 aattgagata gcgattgtaa caggtaaagt aagcaacgaa gaaactgaaa ttttgaaatg
     1741 ggtggataat catgtcaaaa tatagtcaag atgtacttca attactctat aaaaataaac
     1801 cgaattatat atctggacaa agcattgcgg aatcacttaa tatatcgcgc actgcagtaa
     1861 aaaaagtgat tgaccaatta aagttagagg gatgtaaaat agattcagta aatcataaag
     1921 gacatttatt acaacaactc ccagatattt ggtatcaagg tataatagac caatatacaa
     1981 aaagttctgc tttgtttgat tttagtgaag tatacgattc aatagattct acacaacttg
     2041 ctgcgaaaaa atcacttgtt ggaaatcaat cttcattttt tatcttgagt gatgaacaaa
     2101 cgaaaggtcg tgggcgattt aatagacatt ggagttcttc aaaagggcaa ggactttgga
     2161 tgtctgtcgt gttaagacct aacgttgcat tctcaatgat atctaaattt aatttattta
     2221 ttgcattggg gataagagat gcgattcaac attttagtca agatgaagtc aaagtgaaat
     2281 ggccgaatga tatatatatt gataatggta aagtgtgtgg tttcttaact gaaatggttg
     2341 ctaataatga tggtatagaa gcaataatat gtggtatagg tattaatttg acgcaacaac
     2401 tagaagactt tgatgaaagt attagacata gagcaacaag tatacaatta catgataaaa
     2461 ataaattaga tagatatcaa tttttagaga gattacttca agaaattgaa aaaagatata
     2521 atcaattttt aacgttacct ttttctgaaa ttcgtgaaga atatattgca gcttctaata
     2581 tttggaatag aacgttgcta tttacagaaa atgataaaca gtttaaagga caagcaattg
     2641 atttagatta cgatggctat ctaattgtta gagatgaagc gggtgaatca caccgtttaa
     2701 ttagtgcaga tatagatttt taacactaaa gcaaggagag atagctatgg gtatggcaac
     2761 ctatgccgtt gtggatttgg aaacaacagg caaccaatta gattttgacg atatcattca
     2821 aattggtata acgtttgtgc gcaataacca aattattgat acatatcatt ctatgattag
     2881 aacaaattta gagattccgc cattcattca agcgttaaca tccattgaag aaaatatgct
     2941 acaacaagcg ccatatttta atcaagttgc acaagaaatc tacgataaga taaaagattg
     3001 catttttgtc gctcataatg tagattttga tttaaatttt attaaaaagg catttaaaga
     3061 ttgtaatatt caatatcgtc ccaaaaaagt tatagataca ctagaaatct ttaaaatagc
     3121 ttttcctaca gataaaagct atcaattaag tgaattagcg gaggcacatg gtattacctt
     3181 agcaaatgcc catcgagctg acgaagatgc cgctactact gctaaattga tgatattagc
     3241 ttttgaaaaa tttgaaaagt taccgttaga tactttaaag caattatatt atttaagtaa
     3301 acagttgaaa tacgatttgt atgatatttt ctttgagatg gttaggcaat atgatgcaaa
     3361 gccattggat aaatcgtacg aaaagttcga acagattatc tatcgtaagc aagttgattt
     3421 taaaaagccg acaacaaatt ataatggcag cttgaagtct ttatatagca aagctgtaaa
     3481 tcaacttggc ttaacatatc gaccgcagca attatattta gcggaaacta tattagatca
     3541 gctcatgcat agtgaaaaag caatgattga agcatcacta ggcagtggta aatcattagc
     3601 atatttactt gcggccttga tgtataatat tgaaactgga aagcatgtca tgatttctac
     3661 caatactaaa ttactgcaaa gtcaattgtt agaaaaagat attccagcaa tgaatgaagc
     3721 gttgaatttt aaaattaatg cactattgat taaaagtaaa agtgattaca tttcattggg
     3781 acttattagt caaattttaa aagatgacac aagtaattac gaagtgaata tcttgaaaat
     3841 gcaattgctg atttggatta cagagacgcc gtccggagat atacaggaat taaatttaaa
     3901 aggtggtcaa aagatgtatt ttgaccagaa aattgaaaca tatgtaccgg cacgtcacga
     3961 tgttcattat tataatttta ttaagcgaaa tgcacagaac attcaaattg gtattacaaa
     4021 tcatgcacat ttaattcatt ctgatgttga aaattcaata tatcaattgt ttgatgattg
     4081 tatagtggat gaagcacatc gcttaccaga ctatgcctta aatcaagtga caaatgagtt
     4141 gagttatgct gatattaaat atcagcttgg tttgattggt aaaaacgaaa atgaaaaatt
     4201 gttaaaggca attgatcaac tggaaaagca gcgaattttg gaaaaattag atattgcacc
     4261 aattgatatt tttggcttga aggctagtat gaatgagatt catgagttaa atgagcaatt
     4321 gttctcaacg atatttacga ttatcaatga ttctgatgtt tatgatgatg atattcatcg
     4381 cttccataat gtatttactt ttgaaacaaa agacattttg aaggatttac atgcgattat
     4441 cgataagtta aataaaacgc tagaaatatt caatggcatt agtcataaaa cagttaaatc
     4501 attacggaaa cagttgttgt atttaaaaga taaatttaaa aatatcgaac aaagtttaaa
     4561 agctggacat actagtttca tttcaattaa aaatctatct caaaaatcaa caatacgttt
     4621 atatgtgaaa gattacgctg taaaagatgt attaacgaaa caagtattgg aaaaatttaa
     4681 atcactcatt tttatatctg gtactttaaa atttaatcat tcgtttgatg cttttaaaca
     4741 gttattcaac aaagatgttc actttaatac atttgaagtt aacacgtcgt tacaaagtgc
     4801 aaaaaataca agtgtattta taccgagtga tgtagcatct tatcaatata aaaatataga
     4861 tgagtatgta gcatcgattg taagctatat tattgaatat acgactataa cgtcatcaaa
     4921 atgtttagtc ttatttacga gttataaaat gatgcatatg gtacaagata tgctaaatga
     4981 attgccagaa tttgaagatt atgttgtatt aacacaacag cagaaccaaa attataaaat
     5041 agtacaacag tttaacaatt ttgataaggc catactgctt ggtacgtcaa cattttttga
     5101 aggctttgat tttcaagcaa atggtattaa atgtgtgatg atagcgaagt taccgtttat
     5161 gaacaagcat aatgcaaaat attggcttat ggattctgaa ttcacttcaa ctttcaagga
     5221 atatgtatta cctgatgcag ttacacgttt tagacaagga cttggaagat taattagaaa
     5281 tgaaaatgac cgcggcataa tagtttcatt tgatgatcga ctcataaaca gtaattataa
     5341 aaactttttt gagcaaacac ttgaaaatta tcgtcagaaa aaaggcgaca ttcaacagtt
     5401 tggaaaatta ttaagacaaa ttcaaaaaaa gaaacagtaa aatacaaaat aaaattcttg
     5461 taacatgaca tgtgctatgt tctgttacaa gaatttttgt ttggtcgtca aaatttgagt
     5521 tttataatta aacttgaatg ttggcacaaa atcaaccttc atactaataa tattgaggct
     5581 ttattatata ttttgtctga aattgatgat tgcactgtct taatatgaat taatgttcaa
     5641 atctgtgctt aaatcattac catttacacg ttatctttta gtctagttct agaacttgaa
     5701 agcggatttt gttaaaatga atgagtattg tataacgaaa aaataaatag gagaatggtt
     5761 atgaaaacaa cgattaaaca agcgaaagat catttaaacc aagacgttac aattggtgct
     5821 tggctaacaa ataaacgttc aagtggtaaa atcgcctttt tacaattacg tgatggaaca
     5881 ggctttatgc aaggcgtagt agttaaatca gaagttgatg aagaggtatt caaacttgcg
     5941 aaagaaatta ctcaagaatc atctctatat gttacaggca caattacaga agataatcgt
     6001 tctgacttag gatacgaaat gcaagtgaaa tcaattgaag ttatttcaga agcgcatgac
     6061 tatccgatta cacctaaaaa tcatggtaca gaattcttaa tggatcaccg tcatttatgg
     6121 ttacgttcta aaaaacaaca tgctgtaatg aaaattagaa atgaagttat tcgtgcaaca
     6181 tatgaatttt tcaacaaaga tggatttaca aaggttgatc caccaatttt gacagcaagt
     6241 gcaccagaag gtacaagtga attattccat actaaatact ttgatcaaga tgcgttttta
     6301 tctcaaagtg gtcagttata cttagaagct gcagcaatgg cacacggaaa agtattttca
     6361 tttggtccaa ctttcagagc tgaaaaatca aaaacacgta gacacttgat cgaattctgg
     6421 atgattgaag gggaaatggc tttcacaaat catgctgaaa gtttagaaat tcaagaacaa
     6481 tatgtaacac atgtagtaaa atcagtttta gaaaattgta aactagagtt gaaaatttta
     6541 gagcgtgata catcaaaact tgaaaaagtt gcgacaccat tccctagaat ttcatatgat
     6601 gatgcaattg aattcttaaa agcagaaggc tttgatgata ttgaatgggg cgaagatttt
     6661 ggtgcgccac atgaaacagc cattgcaaat cattatgatt taccggtgtt tattactaac
     6721 tatccaacta aaattaagcc tttctatatg caaccaaatc ctgagaatga agaaactgta
     6781 ttatgtgcag acttaattgc acctgaagga tacggtgaaa ttataggtgg atctgaacgt
     6841 gtggatgact tagaattgtt agaacaacgc gttaaagaac atggattaga cgaagaagca
     6901 tatagttact acttagactt acgtcgttat ggtagtgtgc cacactgtgg atttggttta
     6961 ggtttagagc gtacagtagc atggatttct ggtgttgaac acgttcgtga aacagcgcca
     7021 ttcccaagat tattaaaccg tttatatcca taagttttag ggtcgttact taaaatttat
     7081 attatgataa taaaaagaat cgtccagcct atattaatag ctgggcgatt tatgcttgga
     7141 aaatgaagat tgataacggc ttatattatg catgaacaaa ctattgttta gtaactagat
     7201 ttggaaaata aatagctcat ctgattatac ataaagttca ttgttaaaaa ctagttttaa
     7261 aaaaatactg ttatttaaaa tttgtttgct actagttaaa tatattaagc attttagtcg
     7321 aaaattaaag aaaatgattt atactattaa gtaatggtta aatttaaaag ggggcacgaa
     7381 catggataaa tatcaattaa aagcaagacc tgtagtgata cgaagagaat tattagacca
     7441 ttacagtgac ttaggtttag atgagcaaga tttagtcatt ttgcttaaat tgatttatgc
     7501 ttccgaaaca tcaaataaac agccttcaat tgaattactt cagaagggct caactatgca
     7561 accacgcgat attacaatgg ttattcaaaa tttgatacaa cgtgaattgc tagaattaca
     7621 agttcaaaaa gatgaagaag gtagatttac tgaatatatg aatctagatc cttttttcga
     7681 aaaattaagt catatattga agcaacaaag tatggaaacg aaagagcaaa atagtaaaga
     7741 aaaattcaaa caattattta gagttttaga ggatacattt gcaaggccgt tatctccata
     7801 tgaaatagaa acgttaaatc aatggattga tgttgataaa cacgatagtg caattataca
     7861 agcggcatta gacgaggcga atagtttgaa taaacttagc tttaaatata tggatcgtat
     7921 tttacttaat tggaagaaaa acaatgtcaa aacaattgac gattctagaa aaataagaga
     7981 aaaatttaat aaaccgaaaa tgacgcacac agttaaaacg gtacctaaat ttgattggtt
     8041 aaacggggag aaccttgatg gtaagtaaga aaaaagcatt agaaatgatt ggtgttatag
     8101 caaatatgtt tccggatgca gaatgtgaat taaaacatga taatccgttc gaattaacta
     8161 ttgctgtatt attgtcagcg caatgtacag acgttctagt aaatagagta acaactgagc
     8221 tgtttaaaaa atacaaaacg cctgaagatt atttggcagt cagtgatgaa gaacttatga
     8281 atgatattcg ttccatcggt ctgtatcgta acaaagctaa gaatattaag aaactttgtc
     8341 aatctttgat tgatcaattt aatggagaaa taccacaaac acataaggaa ttagaaagtt
     8401 tagcaggtgt aggacgtaaa actgctaatg tagtcatgag tgtagcattt gatgaacctt
     8461 cattagctgt tgatacgcat gtagaacgtg tttctaaacg cttaggtatt aatcgttgga
     8521 aagataatgt gagacaggta gaagaccgtt tatgttctgt catacctaga gatagatgga
     8581 ataggagcca tcatcaactc attttctttg gaagatacca ctgtttagcc cgaaaaccta
     8641 aatgcgatat ttgtccacta ttagaagatt gtagagaagg acaaaaacgt tataaagcta
     8701 gtttgaaaga agcgtgataa catgacaaat attacaaaag aggtatttga taatttagaa
     8761 cgagaaattg atgtatttgc caaaaataag acattaggtt ctaatgaagc aaagccctac
     8821 ttggatgaat accatagtaa aattattgat tattttaagc aggttaatga cataactagc
     8881 aatattgatt ttgataattt aaatcaatat cctgttgtgc caatgaattt taaagaaaga
     8941 tatgattata tgattgaacg taaatatcat tttatgggct atcgacaaat gaagaccttt
     9001 aaaactgaat tgattaaaat gaatgctagt tatcaaacaa ggttaaagaa taagcaggta
     9061 tagacaagtt tgatcatttc ctaaaattga aaatatgtaa aactaattca tgcacacata
     9121 tttgtattta tgttagatgt cgtgtcattg caacgtctta atataacaac tgtccatcta
     9181 catcataatg gatatcttga ggtatgcttt aagatatcta ttttgaatcg taggtggatt
     9241 tcttatatct atgattgtga atatacgttg caaacatgtt gatatttcac tgattaacgt
     9301 actcagggaa ttgataaaca tatcaattta aaacaacgtc taagatgact atgagaaaat
     9361 ataatcataa ttttaaaaat gggaacaacc acttttgcaa tttcaataat tttaaatagc
     9421 taaagatgtg aatatgaaaa actatttaga tgtttcaaaa tgtatgtgaa tgactgattt
     9481 tacggtttct actaatatag caatagcgta tgaatactta taaaaaatcc tcagctacac
     9541 cataaatatg gtataactga ggattttatg ttgagtggat tagttgaata tacctgttaa
     9601 tccaccgctg tttctagatt gttgagatct agtattgtta tttgattgtg cagtaccact
     9661 gctgtttgct gatgtgtcac taccaccgtg tgtactacga tttgtagtgt tgttatcttg
     9721 actaccagaa acattgattg atggaatact accacttact gagctaggac gtttaaagtc
     9781 ttcgccatct ctagatgaaa tttttgacat cacattttca tataagaact gtggatattc
     9841 ttgttggcta tgtcccacaa atgagttttc accatattgt ttaactttac tgaagcccat
     9901 ccacactgac atagtgtatt gaggtgtaaa gccgttaatc cacacgtctt tcgctgcatt
     9961 atcaggtaaa ttatattgtg aataagtttc agcaccgtaa gtaccagtac ctgtcttagc
    10021 ccccatattt actccagata caccatggcc atatgcagaa ccatatggtt taaatgtacc
    10081 ttttagcatc tcagctaaca tgtatgcagt gtaatcactc atcgctttat ggctagtatg
    10141 atcgtattcg attgtttcac catcacgagt aactactttt tgtattgaat gcgcgttgtt
    10201 ataagtacca ccgttagcga ttgcagcaaa tgctgatgct aattgtgttg gtgagaattc
    10261 tgaagcagaa ccaccaagta cttcagatgg accaatatcg ccttcgtagt ttaagccaag
    10321 tttggcagcg aatttcttag gtgcatcatt accagcattt tgcttaactg attgccaagc
    10381 ttttaaagct gggatattga aactttgtcg taaagcatca taaatagata cagtaccgtg
    10441 actcttcaca tcgtagtttc taaatgtaga tccatcaact tgatatgaag attcatcttg
    10501 aatcgcatgg tttgttgccc atttcatatt ttcaatggca ggtccatacg ctaagaaagg
    10561 ttttaaagat gaaccagtag ggtgaggatc tgttgcttgg tttctgttaa cgacgtcttt
    10621 gaaatcacgt ccaccagata tagcaactaa accaccagtt ttactatcaa gaatcgttgc
    10681 accaacttgt tggtctttat tcttgtagaa gctaccatta tcaacatcat tttgtaatgt
    10741 tttttgaaca tctttatcca tgtttgtata aattttaata ccactttgta atacattacc
    10801 taaattttca tctttgaatg ctttattatt cattaattca gattttacaa agttaacgta
    10861 tgaattatac tcagaatctt gatttgtatc aatgttttga cgttcttcag gagtacgatt
    10921 tactaagttc gcttttaaat cgattttctt agcatcttcc cactgtttat ctgtaatgcg
    10981 tttatgataa tgcattaagt ataaaacagt gtttttacga tcttcagcag cttttggatg
    11041 atcataaata ttatagttgt taggaacctg aggtaaaccg gctaaataag cttcttccgc
    11101 taagtttaaa tcttttaaat ctttattaaa gtaatactta gcagcagctt taatacctgt
    11161 tacgccatca gaatagtaaa ttttatttag atatacttgg aagatatcat ctttactata
    11221 ctcttgttct aaacgatatg ataagtatgc ttcttgagct ttacgtccaa tagatttatg
    11281 ttgtgataaa aatgcatctt taacaacttg ttgtgttaat gttgaggcac cttcagaacc
    11341 aaatccacca gtcaagttct taccaattgc accgaataaa cgtttataat caagtgcgcc
    11401 atgttcgtag aaacgattat cttcagttgc aagtactgcg tctttcattg atttcggcac
    11461 gtcttttaaa tttacatgct catgtctttg gccattatct aatgttttaa caagttctcc
    11521 gttcttgtca tatatctttg caggaatcgg atcttgtaat ttagcttcgg taaaagcagg
    11581 tgctttccaa gcataataag caaacaataa gatacctagt aaaagaacaa cgaaaaatgc
    11641 aataatcatg aagccaataa tcttaataat cgttctcttc acatttctat tcttttttga
    11701 gttggatttc ccaccattat taccattttt cttaggctga gaagatcctt tgttttccgt
    11761 catacgcggt cctcactttc atctaatatc aacttatcaa ctgcttttag atagtctaat
    11821 cttggttgat actgataagg aatatggtaa ccattttttc gtatttcatc aactgttata
    11881 gactttttaa tattatcttt atatctcttc caaaatactt cgaattttga atagggtaaa
    11941 agataaactt catctagcgt tttaaaacga atcattaaaa acacaatacc tttttgttga
    12001 tatgcatttt tcatatgttc gacttgatgg tcatgaatat tatttaaagg aaaggacgtc
    12061 ttgtttttag tttcctttgc ttcaaaatca atataataac cttgataaac gccgttgtaa
    12121 tcagttgttg aaggtgtacg aaaataagct tcgttaatca cagctttact ccgtttagga
    12181 tagttgacat taactatttg tactggcgta ggctttttgt gaataactgc aatgtcgctt
    12241 ttaagataaa acgcatttga atgttcgata tctttttcaa gtgacatacc acgtccacca
    12301 tactcaatat tactaaaggc agcggtcttt ttccctccgt ctatagcact atttttacga
    12361 tatggtttac catttggata attcataaat ttcaccacac aagtttggta gtaaacgttg
    12421 tttattatac acaacattat agtgaaacac caaacttaaa ttagtataaa tgcgaaagac
    12481 acaatacacc aaagcttatt ttaacgtgat ttcagcatta acacaataat attataatac
    12541 atttaccgtt aatgttggaa ttttacacac aattttaatt aattgaaaag ttttgcttat
    12601 aaaaatgaat actcattaca atatttttgg attttgttat aaaagtcaaa tcaagatcag
    12661 taattattat ttttgaggca agtacaagta tgtgttg
//
LOCUS       DAHHRR010000074        12481 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.074, whole genome shotgun sequence.
ACCESSION   DAHHRR010000074 DAHHRR010000000
VERSION     DAHHRR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12481
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.074"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..284)
                     /locus_tag="NBI42_001772"
     CDS             complement(<1..284)
                     /locus_tag="NBI42_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1778188.1"
                     /translation="MEKNQRKKRSDAEYNQQIILTTMEDLLEQGEDISAKKMSDIAKI
                     SGVGVGTLYRHFESKTLLCQAIMDKKVDQMFIEIEDILAENTQWPVRDKI"
     gene            complement(558..923)
                     /locus_tag="NBI42_001773"
     CDS             complement(558..923)
                     /locus_tag="NBI42_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCD1778189.1"
                     /translation="MNHQHGDFYYEMNNFFNVYFEYKGFTVNIVSSTIHTHGGGKSLK
                     GSQVSLIIPIDGVDYVAGAGFGDLPFSAMPIVPKDNSPIIHDINGDFHARYVNDYLFY
                     VRKMGKDNDNNWDHTMKRN"
     gene            complement(933..1136)
                     /locus_tag="NBI42_001774"
     CDS             complement(933..1136)
                     /locus_tag="NBI42_001774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778190.1"
                     /translation="MKNAIMKGVVYISFEGLEQYLNGDNTRYNTLSLDVLNYYLKQYV
                     LTIPFKNMDVQNGMKVSAEVKDI"
     gene            1250..2641
                     /locus_tag="NBI42_001775"
     CDS             1250..2641
                     /locus_tag="NBI42_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCD1778191.1"
                     /translation="MRNVYAFNIDLKQRNRVIAVVMIGAFVGVLNQTLMTTILPEIMK
                     DFTVSSSTAQWLTTIFMLVNGIMIPITAFLIERFTLRSLFFNATCFLMIGSFICMLGI
                     NFPMLLVGRSIQALGAGILMPLTQTLLFIMFPPEKRGMAMGMFGLVIGFAPAIGPTAA
                     GWFVNIFDWRYLFLVVLLIGMVDFVFGYLSLPNITELSKPNLEKLSVILSTVSFGGLL
                     YGFSTAGNLGWSHPMVYITIIVAIVILTIFIFRQLKLESPLLEFRVFKYNDFSVAMIL
                     IVLMFMLFIGNLTILPIYMQTMMKWSPLESGLILLPGGLIMGLLSPVTGKLFDKIGGR
                     ILSIMGMLTIMIGALLMAQFSQNTTQLYVIISFSVTMLGNAMIMTPMTTQALNALPRQ
                     YIAHGTAMNNTIRQVSAAIGTGILVTLMTGLGKTSSLSGSQGLIHGLDITFYVVALIA
                     FIGTIIAFFIRKQ"
     gene            2661..3032
                     /locus_tag="NBI42_001776"
     CDS             2661..3032
                     /locus_tag="NBI42_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCD1778192.1"
                     /translation="MLLRHVINTYVGSEIFKSSLPKVQNSDEMAQQFEQGFGLSRRSM
                     RLAGAFEMIGSLLLFTSIFGKLGKKLVVVGTIMINIVMGTAIYNHYKAGHGYKGAQAA
                     SKFFFLNVLSLLEVLSLTRNK"
     gene            3085..4056
                     /locus_tag="NBI42_001777"
     CDS             3085..4056
                     /locus_tag="NBI42_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1778193.1"
                     /translation="MTETKAIDTHAHLWNEDYLDKLGKLGSQGTEVAKGINQSASKED
                     LEKRFKMMDDAGVDLQIISATPQSPQWGTKEEAHQSAQEINDLYERLVQQYPDRFLAY
                     GAVSLPYVDQAIEEAQALLKKDEFVGIAIPTIVKDKVSVADKQFEPFFAAMNELNATL
                     YIHPTGCGAQSPLVNDYQLEWVIGAPLESTFITLQLIKNEIPQKYPNIKFHISHLGGA
                     LPFFMTRIKDNYEDWNAFNIDPYEVLNRQFWYDTANFHEPSLINSIETFGKDKFMMGS
                     DFPYFQDEKYTRGVDYIKNSNIDPDTINGILRGNAIEFYQIEENHSH"
     gene            4132..4518
                     /locus_tag="NBI42_001778"
                     /pseudo
     CDS             4132..4518
                     /locus_tag="NBI42_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4559..4907
                     /locus_tag="NBI42_001779"
                     /pseudo
     CDS             4559..4907
                     /locus_tag="NBI42_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633422.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
     gene            complement(5139..5642)
                     /locus_tag="NBI42_001780"
     CDS             complement(5139..5642)
                     /locus_tag="NBI42_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778194.1"
                     /translation="MIEYYLKKIIHLFENNKCEILHLKKNFNDNFDMLSYIYCIENMH
                     RGSNIIKIAEYILVKYFQKYCIKKDFSIGPFQVKKSFCVSNNLYLESLDKLLELHSSA
                     HVINEFIENKKYYLNNNEILSLYHSGKVMDTSFSTLMYIGLFKHFSSYLRIHEEKTTD
                     DNKLTNY"
     gene            complement(5635..6342)
                     /locus_tag="NBI42_001781"
     CDS             complement(5635..6342)
                     /locus_tag="NBI42_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778195.1"
                     /translation="MKFDLIISNIMIGILVICSAFALMFVFYIQRKLTPEQYFSFSEF
                     FLIGRGLNIKKILNRMILIASFNCILFLILKNFFEIELVCSMCILSTLLGSILIIYPA
                     FKIRHDLPNNEMKSLLNYMYVSFVFLALIISTLTVFTLVVLFDDISIVTLWQEHKSGI
                     ILYLLLVVPSLFFNPTNYEKKMEYAIEKHRESEKEIKETLIINNNETIIPDETKSSSP
                     FKIKKVFRLLNRIMKDD"
     gene            complement(6455..9583)
                     /locus_tag="NBI42_001782"
     CDS             complement(6455..9583)
                     /locus_tag="NBI42_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCD1778196.1"
                     /translation="MSFQFSEDDLEQVALKWFEGLGYSVKNGRDSSDNGIMNERESDK
                     DVVFDDRLEAALRRINPELNNSAIEQAMREISIEKSPSLLENNRSFHEMITNGIEVEH
                     YNDDGETINDLVYVFDFDNPGNNDFLAVNQLTVVNGDYKKRPDIVLFINGLPVVVIEL
                     KNSTNESVGIEDGYHQLETYKMRIPQLFNHNAVLVTSDGVNTKAGSLTADYDRFMTWR
                     TKDGKTEDSSTFRSLDILIHGMLNKDVLLDLIRHFVLFQDDGKGNIVKILAAYHQYYA
                     VNKAIDRAIEATSEHGDGKGGVIWHTQGSGKSLTMVFFSGKLIQKLNNPTLVVVTDRN
                     DLDNQLYGTFVKSKGRSGKGLLRQTPKQAESRKELKELLSVESGGIVFTTMQKFEPEE
                     GQVTMDALTDRKNVVVMADEAHRTQYGFKATYDEKGQGIKYGYAKYLRDALPNASFVG
                     FTGTPVSSTDKNTQMVFGNYIDVYDMTQSVEDGSTVKIYYESRIIPLNLPTDLDIDEA
                     YDVITSGQEEDTKSRLKSKWSRIEALSGAEQRVHALATDIINHFETRQKAMKGKGMIV
                     TMSRRIAVDLYDEIVRLKPEWHSDDDDKGVIKVVMTGSSSDPQNFQKHIGPKKRRNLL
                     ENRMKDVNDELQLVIVRDMWLTGFDVPSMHTMYIDKPMKGHNLMQAIARVNRVFIDKP
                     GGLIVDYVGIAESLKEALKEYTDSDREQTGIDTDKALELMLMKYDIIQDMLYNHDSSD
                     FESEDQLNRYNAISNTMDYIIGLGENERERFVTTVTELSKAFALCATEEAAQELNSEI
                     AFLKAVKSGLVKLLAPPSEGSKRKKTPAEIEAEINQLISKSVVTEEVVDIYQTLGIEN
                     PDISILSDDFLKDVEGLQQKNVAVELLNKLLKGQVKSLMKTNTTVSKRFSEMLSKSIQ
                     KYNNRSIEASKVIEELIQMAKEINQEKERGKELGLSTEEIAFYDALASHETAKEAMDD
                     KELRAIAHELTKTVKNNMSVDWSKRESAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVV
                     EQAELMASQQYK"
     gene            complement(9567..10799)
                     /locus_tag="NBI42_001783"
     CDS             complement(9567..10799)
                     /locus_tag="NBI42_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD1778197.1"
                     /translation="MEFETFNLTDLYTISSGLSKNRKYFGTGTPFLTFKDVFDNLILP
                     NEFSGQVITEEKEREKYSVKKGDLFLTRTSEKQNELGISAVALKDYKNATFNGFTKRL
                     RPNKYCENKLLPVFAAFYFRSNNFRNQVNSMSIMSTRASLNNEMISKLKITIPSLQNQ
                     MKISHILLALLKKEKINQKIIANLEELSQTLFKRWFVDFEFPDENGNPYKSSGGEMVD
                     SELGKIPRSWKVDELGNYIKIKSGKRPKNKVDKEDIENVVPIIGASKIVGYTNDYLYN
                     EKIIIIGRVGTHGVIQRFSTRTWPSDNTFVITSDFESIIYQVLKSIDYISLNRGSTQP
                     LLSQKDIKNTKVVMPTNATLLSKYQKKNNHILKMMDQKIIENKKLTQLRDTLLPKLMS
                     GEIEIPDDIEVNEDELSI"
     gene            complement(10789..12303)
                     /locus_tag="NBI42_001784"
     CDS             complement(10789..12303)
                     /locus_tag="NBI42_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HCD1778198.1"
                     /translation="MASIGFEEKLWQAADKLRGSMDAAEYKNVALGIIFLKYVSDSFE
                     EKYEELLNDEYADEEDKDEYLAENIFWVPKESRWQYINDNSKKPEIGQIIDKAMIAIE
                     RENESLKGVLPKDYARPALDKEKLGDIIDLFTFKVGDSESKKQDVLGRVYEYFIAKFA
                     SAEGKNAGEFYTPASIVKLLVEMVEPYEGRIYDPCCGSGGMFVQSERFVERHQGRLDN
                     IAVYGQESNPTTWKLAKMNLAIRGIDNDLGDHHADTFHNDLHKDLKADFILANPPFNA
                     SDWGREKLLDDYRWKFGIPPKGNANYAWIEHMISKLAPSGTAGFVLANGSMSTSGKDE
                     LEIRKNLIEQDLVECIVTLPGQLFYSTQIPVCLWFVTKNKAKNGKNERRGEVLFIDAR
                     NIGSMVSRTLKEVSDDEIKDIANVYHSWRGTNNNQYEDKAGFCKVAKTEEIKNNEYIL
                     TPGRYVGLAEVEEDSEPFEQKMERITADLSEQFAKSKELEDQIRKSLEGLGYGV"
BASE COUNT         4004 a         2195 c         1725 g         4557 t
ORIGIN      
        1 ttgattttat ctcgcacggg ccactgtgta ttttccgcta aaatatcttc tatttctata
       61 aacatttgat cgactttttt atccataata gcctgacata acaatgtttt actttcaaaa
      121 tgacgatata atgtaccaac tccaacacct gatattttag caatatctga cattttctta
      181 gcacttatat cttcaccctg ttctagtaaa tcttccattg tagttaagat aatctgttgg
      241 ttatattcgg catcactgcg tttttttcgt tgattttttt ccattatatt cctcttttta
      301 acgttgtttt taactttcta atctatcaat tttaacatct aaattaaatt cttcttctaa
      361 aattgtctat cattctcagg tgttacatca tattattctt tcttttgttg cttagttaaa
      421 gttatattat tttgtaacat tgtagcacgc ccaaaaagat ttaatcattg ttactaacta
      481 gcgtttcaca aaaatagcat taggattaat ttcattatat tcaatattgt aaacaaagtc
      541 atgaatatga cgaggtgtta attccgcttc atagtgtgat cccagttatt gtcattatct
      601 ttccccattt ttcgaacata aaataaataa tcgttgacgt accttgcatg gaaatcaccg
      661 ttaatatcat gaatgattgg actattatct tttggaacaa ttggcatggc actaaatggc
      721 aagtctccaa agccggcacc tgcaacataa tcaacgccat caataggtat gattaaagac
      781 acttgagaac cttttaagct ttttccacca ccatgggtat ggatagttga agaaactata
      841 tttacagtaa atcctttata ttcaaaatac acgttaaaga aattattcat ttcataataa
      901 aagtcaccgt gttgatggtt cactacttta ttttaaatat cttttacttc tgcagaaact
      961 ttcatcccat tttgaacatc catatttttg aacgggatag ttaacacata ttgttttaaa
     1021 taataattaa gcacatctaa actaagcgtg ttatatcttg tattatcacc atttaaatat
     1081 tgttctaatc cttcaaaact tatataaact acccccttca ttattgcatt tttcatttta
     1141 tcagatacct attatcatac ccttgagtta ttaaattgac aaacggaaca cgttccgtat
     1201 aaaataaatg atagtaggtt ccacttaaaa gagggggagg cgtcaaaatt tgaggaatgt
     1261 atatgccttt aatatagatt tgaaacaacg gaaccgtgtt atagctgtcg taatgattgg
     1321 tgcatttgtt ggtgtgctga atcaaacttt gatgactacg atacttccag aaattatgaa
     1381 agattttacg gtatcaagta gtacagcaca atggctcacg acaattttta tgctagtcaa
     1441 tgggattatg attcctatca cagctttttt aattgaacgt tttacattac gttctttatt
     1501 ttttaatgca acatgctttt taatgattgg ttcatttatt tgtatgctag gaatcaattt
     1561 tccaatgtta ttagtaggac gtagtattca agccttaggt gctggtatac tgatgccact
     1621 cacacagaca ctccttttca ttatgtttcc acccgaaaaa cgtggaatgg caatgggaat
     1681 gttcggtctt gtaataggtt ttgcacccgc gattggacca actgcagctg gttggttcgt
     1741 caatattttc gattggcgct atctattcct agtcgtatta ctaataggta tggttgattt
     1801 tgtattcggg tatctatcat tgccaaatat aacggaacta agtaaaccaa acttagaaaa
     1861 actatccgtt attttatcga cggttagctt tggtggctta ctatatggtt ttagtacagc
     1921 aggaaactta ggttggtcac atccaatggt ttatataact attattgttg ccatagttat
     1981 attaacgata tttattttcc gacaattaaa actggaatcg ccattgttag agtttagagt
     2041 atttaaatat aacgactttt cagtggcaat gattttaatt gtactaatgt tcatgttatt
     2101 tatagggaat ttaacaatct taccaattta tatgcaaacg atgatgaaat ggtctccgtt
     2161 agagtcagga cttattttat tacctggcgg gttaattatg ggactgttat cgcctgtcac
     2221 tggtaaatta ttcgataaaa ttggtggacg tatcttgagt attatgggga tgctaacgat
     2281 tatgattggc gcattattaa tggctcaatt tagtcaaaat acaacacaac tatatgtgat
     2341 tatttcattt tcagttacca tgttaggtaa tgctatgatc atgacgccca tgacaactca
     2401 agctttaaat gctttgccac gtcaatacat tgcacatggt acagccatga ataatacgat
     2461 tagacaagta tcagctgcaa taggtactgg tatcttggtc acattgatga caggattagg
     2521 taaaacctct tcattaagtg gtagtcaagg acttattcat ggtctagata ttacattcta
     2581 tgtcgtcgca ttgattgcct ttataggtac tatcattgca ttctttattc gcaaacagta
     2641 attaattgga ggattttaaa atgttattaa gacacgtcat taacacatat gtaggtagtg
     2701 aaatttttaa aagtagttta ccaaaagtac aaaatagtga tgaaatggct caacaatttg
     2761 aacaaggctt tggtttatca cgtagatcaa tgagattagc tggcgctttc gaaatgattg
     2821 gttcacttct tttatttact tctattttcg gtaaattagg aaaaaaatta gtagttgttg
     2881 gtacgattat gattaatatt gttatgggta cagctattta taaccattat aaagctgggc
     2941 atggttataa aggtgcacaa gctgcttcta aatttttctt tttaaatgta ttaagcttac
     3001 tagaagtgtt aagcttaact cgtaataaat aatctattgg caataagcaa attatgaatg
     3061 acaattttta atggaggggt taatatgact gaaacaaaag cgattgatac acacgcacat
     3121 ttatggaatg aagactattt agacaaactt ggcaaattag gtagtcaagg tactgaagtt
     3181 gctaaaggga ttaatcaatc tgcgtctaaa gaagatttag agaaacgctt caaaatgatg
     3241 gacgatgctg gcgtagattt acaaattatt tctgcgacac cacaatctcc acaatggggt
     3301 actaaagaag aagctcacca aagtgctcaa gaaattaatg acttatatga aagattagtt
     3361 caacaatatc cagatcgttt cttagcatat ggtgctgtct cactacctta tgtcgatcaa
     3421 gctattgaag aagcccaagc ccttttgaaa aaagatgaat ttgtgggcat tgccattcca
     3481 actatagtaa aagataaggt ctctgttgct gataaacaat ttgaaccttt ctttgcagcg
     3541 atgaatgagc tcaatgctac actttatatt cacccaacag gttgtggcgc tcaaagtccg
     3601 ttagtcaatg actatcaatt agaatgggtt attggtgcac cgttagagtc tacctttatt
     3661 acattacaat tgattaaaaa tgaaattccg caaaaatatc caaatattaa attccatatt
     3721 tcacatttag gtggcgcttt accattcttc atgactcgta ttaaagacaa ttatgaagat
     3781 tggaatgcct ttaatattga tccttatgaa gtattgaatc gacaattttg gtatgataca
     3841 gcaaacttcc acgaaccttc attgattaac tctattgaga catttggtaa ggataaattt
     3901 atgatggggt cagacttccc ttacttccaa gatgaaaaat atacacgcgg cgtagactat
     3961 attaaaaaca gtaatattga ccctgacacg attaatggta tattaagagg caatgcaatt
     4021 gaattttatc aaattgaaga aaaccatagt cactagcgtt ttattctaga ggcattatac
     4081 tatggtataa actaaataag ttagaataac gctactttac tttctatata aaaaggtatc
     4141 attaagtatt tattagatca agatgactca ctaaatgacg cctaccaata tatcaatcag
     4201 ctatgattca aacttaaaca taatgattat gaaggcttta ttcacgaatt aaaacatatg
     4261 ccattatccc aaacgcattc agttgtccaa cgagcaatta agacacttaa caaacatgta
     4321 tactatataa aaaatacatt tgattattac aatttatcaa atggtccttt agaagggata
     4381 aacaacaaaa taaaattaat taaacgcaca tcttttggtt atggaaatta caatcatttg
     4441 cgcaatcgaa tattattatg ttcaaaactt tacgctccaa aaagtaaaaa ggaagttaag
     4501 caatgtttag ttgtttaact tcctttattg aatgcatcag tccaatttga catagagcca
     4561 aaataatcca gctaatatat taataatcaa tgaaatagga aaaaatacaa ataactatgt
     4621 catggtctat agagataatc ctaaggataa aaaactacaa ctcacttcaa gcctaaaatg
     4681 aaaaaaataa tatatctgag tccgttaagc atcaggataa atatgaagtg aagaatactg
     4741 ataaggcaac acagcaaacc caaaaagaag tttgggtttg gaaatcagat ttatataaat
     4801 cattactaag cttcgatgaa agtgaaaacg aattaattca atcaacaact acagttaccg
     4861 ttccaaaaga tacttgggtt atcataaatg ccaaaaaaga caaatagaaa atacagattt
     4921 atttagctat gccaaatttt aggaatagca aaaaacacct tatatgattc aaattaagca
     4981 gaagcaagag cacgagcact actaaatgaa tttgaagccc aaatggacga agacgttaaa
     5041 gtagaattat aggtgtaata agggaaagga ggaatttaag tttcctcctt ttttgtttgt
     5101 gttaaagtct tttagatatg aactagggtg atttaaattt aataatttgt taacttgtta
     5161 tcatcagttg ttttttcttc gtgtattctt aaataactag aaaaatgttt aaataatcct
     5221 atatacatta aagttgaaaa gctagtatcc ataaccttcc ctgaatgata taaggataaa
     5281 atctcattat tattcaaata atatttttta ttttcaataa attcattaat tacatgagca
     5341 ctactgtgca actccaataa tttatctagt gactctaaat ataaattatt agacacacaa
     5401 aaactttttt taacttgaaa tggacctata gaaaagtctt tttttataca atatttttga
     5461 aaatatttta ctaatatata ctccgctatc tttataatat ttgaacctct atgcatgttc
     5521 tcaatacaat aaatataact taacatatca aagttatcat taaaattctt ttttaaatgt
     5581 aaaatttcgc atttattatt ttcaaataaa tgtataattt tcttcaaata atattcaatc
     5641 atctttcata atcctattca acaatctaaa aactttttta attttgaatg gtgaagaact
     5701 tttcgtctcg tctggtataa ttgtttcatt attatttatt attagcgttt cctttatctc
     5761 tttttcactc tcacgatgtt tttcaatggc atattccatt ttcttttcat aatttgtagg
     5821 attaaaaaat agactaggta ccactaatag aagatataaa attattccac ttttatgctc
     5881 ttgccaaagt gttactatac taatatcatc aaataatact actaaagtaa atacagtaag
     5941 agtagaaata ataagcgcta aaaaaacaaa agatacatac atatagttca acaaactttt
     6001 catctcattg ttaggtaaat catgcctaat tttaaacgcc ggataaataa ttaaaatcga
     6061 acccaacaat gtacttagta tacacatact acatactaat tcaatttcaa aaaaattctt
     6121 aagtattaaa aacaaaatac agttaaaact agcaatcaat atcattctat tcaaaatttt
     6181 cttgatattt aaacctcttc ctattaaaaa gaattctgaa aaagaaaagt attgttccgg
     6241 cgttaacttt ctttgaatat aaaatacaaa cattaaggca aatgcagaac aaatcactaa
     6301 tatccctatc ataatattac ttataattaa gtcgaatttc atatcaatct ccttttctct
     6361 ttagtctacc ggtatattga cactttttta tatactctgt cttaaatgtt taattttaaa
     6421 aaagtatcta acaggtcaac aatataaaca gtatttattt atattgttga cttgccatta
     6481 attctgcttg ttccactact tgttctactg ccattttttg taagtctggt ggataaccat
     6541 atttctttaa tagtcgtctt acttggactc tcattttagc ctttgcgctc tcacgttttg
     6601 accagtctac gctcatatta ttcttaaccg tttttgttaa ttcatgtgca atagccctta
     6661 attctttatc atccatcgct tcttttgctg tttcatgtga agctaatgcg tcataaaatg
     6721 caatttcttc agtacttaaa cctaattctt ttcctctttc tttttcttga tttatttctt
     6781 tcgccatttg aattaattct tctatgactt ttgatgcttc aatagaacgg ttattatatt
     6841 tttgtattga tttacttagc atctcagaaa accttttaga tacagtagta ttagttttca
     6901 ttaatgactt aacttgtcct ttgagtaatt tgttcaacaa ttcaactgca acattttttt
     6961 gctgtagtcc ttctacatct tttaagaaat catccgacaa tattgagata tctggatttt
     7021 caattccgag tgtttgataa atatctacaa cttcttcagt tacaactgac tttgaaatca
     7081 attgatttat ttctgcttct atttcagcag gtgtcttttt acgcttgctt ccttcactag
     7141 gtggagctaa taatttaaca agtcctgatt ttactgcttt taaaaacgca atttcactat
     7201 tcagttcttg agcagcttcc tctgttgcac ataatgcaaa agccttagaa agttctgtaa
     7261 ctgttgtcac aaatcgctct ctttcattct cacctaatcc aatgatataa tccatcgtat
     7321 ttgagatagc attatagcga tttaattgat cttctgactc aaagtcagaa gagtcatggt
     7381 tatatagcat atcttgaatg atatcgtatt tcattaacat tagttctagc gctttatctg
     7441 tatctatacc tgtttgttct ctatcagagt ccgtatactc ttttagtgct tccttcagac
     7501 tttcagcaat acctacatag tcaacaatta agccacccgg tttatctata aatactctat
     7561 taacacgtgc aatagcttgc attaagttgt gacctttcat cggtttatct atgtacattg
     7621 tatgcattga aggtacatca aatcctgtta accacatgtc tcgcacaatt acgagttgca
     7681 attcatcatt aacatctttc atacgattct ctaaaaggtt tcttcgcttt ttcggaccta
     7741 tatgtttttg gaagttttgt ggatcactag atgaacctgt cataacaact ttgataacac
     7801 ctttgtcatc atcatctgaa tgccattcag gtttaagtct tacaatttca tcatataaat
     7861 cgactgcaat acgacgactc attgtgacaa tcataccttt acccttcatt gccttctgtc
     7921 ttgtttcaaa gtgattaata atatcagtcg ctaaagcatg tacacgttgt tctgcaccag
     7981 atagagcttc tatacgtgac cacttagatt tcaatcgaga tttagtatct tcttcttggc
     8041 cagaagtaat gacatcatat gcttcatcaa tatctaaatc ggttggtaaa tttaatggaa
     8101 taatacgact ttcatagtat attttaaccg tactaccgtc ttctacagat tgtgtcatat
     8161 catatacatc aatataattt ccaaatacca tttgtgtatt cttatctgtt gaagatacag
     8221 gtgtaccagt aaaaccaacg aacgaagcgt taggtagagc atctcttaaa tattttgcat
     8281 aaccatattt aataccttga cctttttcat cgtaagttgc tttgaagcca tattgtgtac
     8341 ggtgtgcttc gtctgccata actactacat ttttacggtc tgttaatgca tccattgtca
     8401 cttgaccttc ttcaggttcg aatttctgca tagttgtaaa tacgatacca cctgattcta
     8461 cagataatag ttctttcaat tcttttcgtg attcagcttg tttaggtgtc tgtcttagta
     8521 atcctttacc tgagcgtcct ttggacttaa caaaagtacc atacaattga ttgtctaagt
     8581 cattacggtc tgttacgacc actaatgtcg ggttatttaa tttttgaatt aactttcctg
     8641 agaagaagac cattgtcaaa ctcttaccag aaccttgtgt gtgccaaata acaccacctt
     8701 taccatcacc atgttcagat gttgcttcaa tagcacgatc aatagcttta ttcactgcat
     8761 aatattgatg atatgcagct aatattttaa ctatatttcc tttaccatca tcttggaaca
     8821 aaacaaaatg acgaattaaa tctaatagta catccttatt taacataccg tgaatcaata
     8881 tatctaaact acggaacgtt gatgaatctt cagtttttcc atctttagta cgccaagtca
     8941 taaaacggtc ataatcagca gttaaagaac cagcttttgt attaacacca tcactcgtta
     9001 ctaatactgc attatgatta aataattgtg gaattctcat tttataagtt tctaactgat
     9061 gatacccgtc ttcaatacca acagattcat ttgtagaatt ctttagttca attaccacta
     9121 ctggtaatcc atttatgaat aacacaatat caggtctctt tttataatct ccattcacaa
     9181 cagttaattg attaaccgct aagaaatcat tatttcccgg gttatcaaag tcaaacacat
     9241 agaccaaatc attaatcgtc tcaccatcat cattatagtg ttcaacctca ataccattag
     9301 taatcatttc atggaaagaa cgattattct ctaataaact aggagacttc tcaatagaaa
     9361 tttcacgcat agcctgttca attgcactat tatttagttc aggattaata cgtctcaaag
     9421 ctgcttccaa acgatcatca aacacaacat ctttgtcgct ctcccgttca ttcataatcc
     9481 cattgtcact actatctcga ccattcttaa cagaatagcc aagaccctca aaccacttca
     9541 gtgcaacctg ttctaaatca tcttcactaa attgaaagct catcttcatt cacctcgatg
     9601 tcatcaggta tctcaatttc acctgacatt aattttggta gtaatgtgtc acggagttgt
     9661 gttaatttct tgttttctat gattttttga tccatcattt ttaaaatatg attgtttttc
     9721 ttttgatatt ttgaaagcaa agttgcattg gtgggcatta ctactttagt attcttaata
     9781 tctttttgag ataatagagg ttgtgtagaa cctctattca atgatatata gtctatactt
     9841 ttaagtactt gatatataat gctttcaaaa tcacttgtta ttacaaaagt attatctgat
     9901 ggccatgttc ttgtagaaaa cctttggatt acaccatgag ttcctaccct accaattata
     9961 ataatttttt cattatataa ataatcattc gtataaccca ctattttact agcaccaatt
    10021 attggcacca cattttctat atcttcttta tcaactttat ttttaggtct tttaccactt
    10081 tttattttta tataatttcc taattcatca actttccaac tcctaggtat tttacccaat
    10141 tcactatcaa ccatctctcc accgcttgat ttgtatggat ttccattttc atcaggaaat
    10201 tcaaaatcaa caaaccaacg tttgaatagt gtttgagaaa gttcttcaag atttgctatg
    10261 attttttgat tgattttttc tttttttaaa agtgctaata gtatatgact aattttcatc
    10321 tgattttgaa gactaggtat agtaatttta agttttgaaa tcatttcgtt atttaaactt
    10381 gctcttgtag acatgataga catagaattt acttgatttc taaaattatt acttctaaaa
    10441 taaaatgcag caaatactgg taacaattta ttctcacaat atttgtttgg tcttaatctt
    10501 ttggtaaatc cattaaaagt cgcattttta tagtctttta aagctactgc acttatacct
    10561 aactcatttt gtttctcact agttcttgtt aaaaaaaggt ctcctttttt aactgaatac
    10621 ttttctcttt ctttttcttc agttataact tgcccagaaa actcattagg taatattaaa
    10681 ttatcgaata catctttaaa tgttaaaaaa ggtgtaccag taccaaaata ttttctattt
    10741 tttgataaac ctgaactaat agtatataaa tcagttaaat taaatgtttc aaactccata
    10801 acctaatccc tccaatgact tacggatttg gtcctcgagt tcttttgatt tcgcaaattg
    10861 ttcacttaaa tctgctgtga ttctttccat tttctgttca aatggttctg agtcttcttc
    10921 tacttctgct aatcctacat aacgtcccgg cgttaagata tattcattgt tttttatttc
    10981 ttctgtcttt gctactttac aaaaacctgc tttatcttca tattggttat tgtttgttcc
    11041 acgccatgag tgataaacat ttgctatatc ttttatttca tcatctgaga cttcttttaa
    11101 tgttcttgat accatgcttc ctatatttct agcatctata aatagtactt ctccacgtcg
    11161 ttcattcttg ccgtttttag ctttattttt tgtcacgaac cacaaacata ctggaatttg
    11221 agttgaatag aatagttgcc ctggtaatgt aactatacat tcaactaagt cttgttcaat
    11281 taagttttta cgtatttcta attcatcttt accacttgta gacattgaac cattcgctaa
    11341 tacaaatcct gctgtaccac ttggggcaag tttagaaatc atatgttcaa tccatgcata
    11401 gttggcattg cctttaggtg gaataccaaa tttccagcgg taatcatcta gtaatttttc
    11461 tcgtccccaa tcacttgcat tgaatggtgg attagccaat ataaaatcag ctttcaaatc
    11521 tttatgtaaa tcattatgaa acgtgtctgc atgatgatca cctaaatcat tatctatacc
    11581 tcgaatagcc aaattcattt ttgctaattt ccaagtggtt ggatttgatt cttgaccgta
    11641 taccgcaata ttatctaagc gtccttggtg acgttctaca aaacgttcac tttgtacaaa
    11701 cataccgccg gaaccacagc aggggtcata gattctacct tcatatggtt caaccatttc
    11761 aacaagtaat ttaactattg aagctggtgt atagaattca cctgcatttt taccttcagc
    11821 gcttgcaaac ttcgcgatga aatattcata aacacgtcct aagacgtctt gttttttact
    11881 ttcagaatcc ccaactttga aagtaaatag gtcgataata tctcctaatt tctctttatc
    11941 taatgcaggt cttgcataat ctttaggtag cactcctttt aaagactcat tttctctttc
    12001 aattgctatc atagctttat cgataatttg accaatttct ggtttcttag aattatcatt
    12061 tatgtattgc catcttgatt ctttaggaac ccagaaaata ttttctgcta agtattcatc
    12121 tttatcttct tcatcggcat attcatcatt taataattct tcatactttt cctcaaatga
    12181 atcagaaaca tatttcaaaa atataatacc taatgctaca tttttatatt ctgctgcatc
    12241 catactccca cgtaatttat ctgcagcttg ccataatttc tcttcaaacc caattgacgc
    12301 catttatatt ccacctctat attatccttt atacaaatat accaaagatt aaagaactat
    12361 gtgaatgatt aaaacaatta aaataaaatg tcattttaaa aaattatatg gtattttaat
    12421 ataaatagtg tatattggat gttttaatat aggattataa aaagtacaaa ataaagttgg
    12481 t
//
LOCUS       DAHHRR010000075        12388 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.075, whole genome shotgun sequence.
ACCESSION   DAHHRR010000075 DAHHRR010000000
VERSION     DAHHRR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12388
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.075"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(204..563)
                     /locus_tag="NBI42_001785"
     CDS             complement(204..563)
                     /locus_tag="NBI42_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCD1778199.1"
                     /translation="MNIVSTILIIFVAIEFFYIMYLETFATTSRKTSETFNISIGKLK
                     DKNIKLLLKNQGVYNGLIGVLLLYGLFFSSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            complement(582..1427)
                     /locus_tag="NBI42_001786"
     CDS             complement(582..1427)
                     /locus_tag="NBI42_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1778200.1"
                     /translation="MNIMLTGATGNLGKHITKQAIDNHIDHFYIGIRNIEKMPKEWDG
                     KVSVRQLDYFNQASMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKKSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAERLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGIIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            1887..2567
                     /locus_tag="NBI42_001787"
     CDS             1887..2567
                     /locus_tag="NBI42_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HCD1778201.1"
                     /translation="MKFKTIAKASLALGMLATGVITSNTQSVQAKTEVKQQSESELKH
                     YYSGPSFEHKKVTGFKYTEDGKHYLEVTVGQQHSRITLLESDKDKFKEGENSNIDVFV
                     VREGAGRQATNYSIGGVTKTNSVQYIDYINTPILEIKKGNEDAQTIYYQIYKEDISLK
                     ELDFKLRKQLISQSGLYSNGLKQGQITITMKDGTTHTIDLSKKLEKERMGESIDGTNI
                     EKILVEIK"
     gene            2820..3515
                     /locus_tag="NBI42_001788"
     CDS             2820..3515
                     /locus_tag="NBI42_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HCD1778202.1"
                     /translation="MKMKSIAKVSLVLGILATGVNTTTEQPVHAEKKPIVISENSKKL
                     KSYYTQPSTEYKNTTGYISSIQPDIKFMNIIQNNTVNNLALVGKDKQHYHAGVHRNLN
                     IFYVAEDKNFNGGKYSIGGITKASDKAVDQIAEARVIKEDHTGEYDYDFFPFKVDKED
                     MSLKEIDFKVRKYLIENYGLYGEMSSGSIIVQTKNYGRYTFELDKKLQEDRMSDIIDA
                     TSIERIEVKVVKA"
     gene            3803..4648
                     /locus_tag="NBI42_001789"
     CDS             3803..4648
                     /locus_tag="NBI42_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HCD1778203.1"
                     /translation="MKMTTIAKASLALGLLTAGVTTTQTANAEKASTANPEQAPKASA
                     EKASTPSSTNIKVTQPTSTATTPSSSNVDTPQPQSTNPATPPIEINPKYKDLRAYYTK
                     PSFEFEKQFGFMLKPWTTVRFMNIIPNRFIYKIALVGKDDKKYKDGPYDNIDVFIVLE
                     DNKYQLKKYSVGGITKTNSKKVDHKAELNITKEDNKGKISSDVSEYKITKEEISLKEL
                     DFKLRKQLIEQHNLYGNIGSGTIVIKMKNGGKYTFELHKKLQEHRMADVIDGTNIDSI
                     EVNIK"
     gene            4770..4913
                     /locus_tag="NBI42_001790"
     CDS             4770..4913
                     /locus_tag="NBI42_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778204.1"
                     /translation="MFVKVAFLWLKSDETSNMPSVDSHQNQFYLTNIMDFLTMIQIKL
                     NKV"
     gene            5007..5939
                     /locus_tag="NBI42_001791"
     CDS             5007..5939
                     /locus_tag="NBI42_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HCD1778205.1"
                     /translation="MKMTTIAKTSLALGLLTTGVITTTTQAANATTPSSTKLETPQPT
                     PTSTKTEQSPKANAENVLATNPEQSPKANAEKATTPASTDIKTPQSTPNATTPPSIKV
                     ETPQSPNTKQVPTEINPKYKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNIVPDYFI
                     YKIALVGKDDKKYGEGVHRHVDAFIVLEEKNKYEVEIYSVGGITKSNSKKVDHKAGIR
                     ITKEDNKGTISHDVSEYKITKEEISLKELDFKLRKQLIEKHNLYGNVGSGKIVIKMKN
                     GGKYTFELHKKLQEHRMADVIDGTNIDSIEVNIK"
     gene            6304..7008
                     /locus_tag="NBI42_001792"
     CDS             6304..7008
                     /locus_tag="NBI42_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HCD1778206.1"
                     /translation="MKMTAIVKASLALGILATGTITSTHQTVNASEHESKYENVTKDI
                     FDLRDYYSGASKELKNVIGYHYSKGGRHYLVIDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVYSYGGVTKKNKGAYYDYLNAPRFQIKKDEGDGIATYDRIHYI
                     YKEEVSLKELDFKLRQYLIQNFGLYKKFPKDSKIKVTMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEVDIR"
     gene            7454..8149
                     /locus_tag="NBI42_001793"
     CDS             7454..8149
                     /locus_tag="NBI42_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HCD1778207.1"
                     /translation="MKLKTIAKATLALGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELKNLDGLYRQKVTDKGVYVWKNRKDYFVGLLGKDIEKYPQGEHDKQDAF
                     LVIEEETVNGRQYSIGGLSKTNSKEFSKEVDVKVTRKNDESSEKSKDSKFKITKEEIS
                     LKELDFKLRKKLMEEEKLYGAVNNRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTIDG
                     TKIKEINVELEYK"
     gene            8560..9255
                     /locus_tag="NBI42_001794"
     CDS             8560..9255
                     /locus_tag="NBI42_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HCD1778208.1"
                     /translation="MKLKTLAKATLALGLLTTGVITTEGQAVHAKDKQERVQHLYDIK
                     DLHRYYSAPSFEYSNINGKVENYNGSNVVRFEQKDQNHQLFLLGKDKEEYKEGLKGQN
                     VFVVQELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFLINKEEV
                     SLKELDFKIRKQLVEKYGLYKGTTKYGKITINLKDEKKEVIDLGDKLQFERMGDVLNS
                     KDIQNIAVTINQI"
     gene            9586..10284
                     /locus_tag="NBI42_001795"
     CDS             9586..10284
                     /locus_tag="NBI42_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HCD1778209.1"
                     /translation="MKLTAIAKATLVLGILTTSVITAESQTVHAKGKYEKMNRLYDTN
                     KLHQYYSGHSYELTNVSGQSQSYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGITKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EELSLKELDFKIRKLLIKKYKLYEGAADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVNLK"
     gene            10660..11345
                     /locus_tag="NBI42_001796"
                     /pseudo
     CDS             10660..11345
                     /locus_tag="NBI42_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
     gene            11703..12386
                     /locus_tag="NBI42_001797"
     CDS             11703..12386
                     /locus_tag="NBI42_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HCD1778210.1"
                     /translation="MKLTAIAKATLALGILTTGTLITEVHSGHAKQNQKSVNKHDKEA
                     LHRYYTGNFKEMKNINALKHGKNNLRFKYRGMKTQVLLPGNEYRKYQQRRYTGLDVFF
                     VQERRDKHDISYTVGGVTKTNKTSGFVSEPRLNVTKENGKDAFVKGYPYHINKEEISL
                     KELDFKLRKHLIEKYGLYKTISKEGRVKISLKDGSFYNLDLRYKLDFKYMGEVIDSKQ
                     IKDIEVNLK"
BASE COUNT         4964 a         1683 c         2116 g         3625 t
ORIGIN      
        1 aatacatata agaaaaatgc agtaagttcc aatgcgagta gttataataa tattgcgttt
       61 aattgttaat tagtaatcat actaatcctt gatttggaag ttttgctcat ttattcgtga
      121 gtctaaagtc cacacccaaa tcactccaaa ccctcaaatc ctccaaacct ccgactacat
      181 ctccaaacca caaacatcaa tacttaccaa agcattgaaa tgagtgccaa tactggcaac
      241 gtgccttgtt tgaaaaagat actaatattg cttgaaaggc caccataaat agcaacgcca
      301 atgatataca ctaaaatagc agcgcatatt tcttttgggt tactactgaa aaataaaccg
      361 tataataaca aaacgccaat caaaccgtta tatacgcctt gattcttcaa aagcaattta
      421 atatttttgt ctttcaattt accaatgcta atattaaatg tttcacttgt tttccttgaa
      481 gttgttgcga atgtttcgag atacataata tagaaaaact ctattgccac aaatatgatt
      541 aaaattgttg agacgatatt cactgtaacg ctcctttatt attaaatatt ttcttgtaaa
      601 aatgattgta gtgtctgtgg ttgatcattg actaattgtt ggaaatcatt ggattcttgg
      661 tctaatagtc ctcttgctcc tgcgtcgtac attgatgcca ataatgcacc aaaaccttta
      721 ggttcatcgt acatttctgc aaatgtctct aatgaaacgg gctcatattt aatttctgtg
      781 cctgatgcct cagataaaat agcagcaagt tccttcatat cataactgta gcctgataat
      841 aaataacgtt tgccccaagt atctggattt ttaataatag caatgatacc tctagcaata
      901 tcatttctag taatataatt aatacgacca tcaccagctg gataaatcag tttgtgcata
      961 ttcatcaatt ctggtaaata tggtttaagt ggatccatgt acattgccat tcttacatac
     1021 gtatagtcaa taccacttgt tgctaataac cgttcggcat aaccgaaata agggctcatg
     1081 tggaatggat tattatgttg atccgcgtaa taacctatga aaatgatatg agcaacgcca
     1141 cttttctttg cagcatatac taaattttct acttcaggaa tacgtttgaa tgatggatgg
     1201 ataatacttg gaataaacac aacggtatcc attcctttaa atgcttctac catgcttgct
     1261 tgattaaaat aatctaattg tcgaacagat actttaccgt cccattcttt tggcattttc
     1321 tcgatatttc taataccaat gtaaaaatga tctatgtgat tatcaatggc ttgcttcgta
     1381 atatgtttgc ctaaattgcc tgtagctcct gttagcataa tattcatttg cgtcatctcc
     1441 taatattgat atacataaca taatacttat tcgatgattt tcaaaatatt tgattttata
     1501 aaaagtgtag tctgcattta tggtcataat gtttagtaaa tacttaacat cggaaacgcc
     1561 gtaatgatgc gtttttgttg atgtgtatca ctaatatctt tgaaaatttt aatcaggtac
     1621 tacgacaata tgatgtctgt tttgtgtctg aaagttttac agtttttaaa attaaattgg
     1681 aataaagtgt gaatggtata aaaatgagtc tcgatggata agaattgatt aataacagtt
     1741 agcatttatt aattacctta acaatgattc aagtttagtt aaacgaggtt taattggaat
     1801 ggcatattgg cgcgaatata atgtgggtag attgttcata ttacgtaatt gaaaaatcaa
     1861 atttaaatag attggggctt aaaattatga aatttaaaac aatagcaaaa gcaagtttag
     1921 cattgggaat gttagcaaca ggtgtaatta cgtcgaatac acaatcagta caagcgaaaa
     1981 cagaagtaaa acaacaaagt gaatcagagt tgaaacacta ttatagtgga ccaagttttg
     2041 aacataaaaa agttactggg tttaaatata ctgaagatgg taagcattat ttggaagtta
     2101 cagtaggaca acaacattct cgaataactt tactagaatc tgataaagac aaatttaaag
     2161 aaggagaaaa ttctaatata gatgtatttg tcgttagaga aggtgctgga agacaagcaa
     2221 cgaattactc aattggtggg gttacaaaaa caaatagtgt tcagtatatt gactatatca
     2281 atacaccaat tttagaaata aagaaaggta atgaagatgc tcaaacaatt tattaccaaa
     2341 tttataaaga agacatttcg ctaaaagaac ttgattttaa attgagaaag caattaatta
     2401 gtcaaagtgg cttatattca aacggtctta aacaaggtca aattacgatt acaatgaagg
     2461 atggcacaac acatacaatc gatttaagta aaaaacttga aaaagaacgt atgggcgagt
     2521 caatcgatgg cactaatatt gaaaaaattc tagtagaaat taaatagtac ctgttacaga
     2581 aatatatgga agttaagcga cacatcgttg cttagcttct tttttgaggg gcaaagtgac
     2641 ttaacataca cacaaacaaa ctcattactc attatctttt acttaactag cttaatcata
     2701 ttttatgaat agttaaaaaa ggtttaatgt gaatatcagg acacagctcc tataatatgg
     2761 ttgtatggtt caaattacgt aataagacaa tcgaacataa tagattggag catacaacta
     2821 tgaaaatgaa atcaatagca aaagtaagtt tagtattagg tattttagca acaggtgtaa
     2881 acactacaac ggaacaacca gtgcatgctg aaaagaaacc tattgtaata agtgaaaata
     2941 gtaaaaaatt aaaatcgtac tatactcaac ctagtactga atataaaaat acaacaggtt
     3001 atatcagtag catccaacca gatattaaat ttatgaatat catacagaat aatactgtga
     3061 ataatctagc tttagttggc aaagataagc aacactatca tgcaggtgta catcgtaatc
     3121 ttaatatatt ttacgtggct gaagataaga attttaatgg tggaaagtac tctattggtg
     3181 gtatcacaaa agcaagcgat aaagctgttg accaaatagc agaagcaaga gttattaaag
     3241 aagatcatac tggtgaatat gattatgact ttttcccatt taaagtagat aaagaagata
     3301 tgtcattgaa agagattgat tttaaagtaa gaaaatatct cattgaaaat tacggtcttt
     3361 acggtgaaat gagttcagga agcataatcg ttcaaacgaa aaactatggc aggtatacat
     3421 ttgaattaga taaaaagtta caagaagacc gtatgtccga tatcatcgat gctacgagta
     3481 ttgaaagaat tgaagtcaaa gtagtaaagg cataacgtat agacttgctg tcgacataat
     3541 ttgaaataga aaaagagaag ttaagtgatg gtaatacgtt gcttaatttc ttttttgtgc
     3601 cttaaaatca ttcgatcata aatagaatcg ctacataaaa ctcaaaagta ctaattactt
     3661 tttacttaaa ttacttaata atacttcaaa aactgttaaa cggtgtttaa tgtgaatatc
     3721 ctagactgtc ttttataatg aagttgtatg gttcaaatta cgtaataaaa caatcgaata
     3781 taatggattg gagcatacaa ctatgaaaat gacaacaatt gctaaagcaa gtttagcact
     3841 aggactttta acagcaggag taacgacaac gcaaacagct aacgcagaaa aagcatcaac
     3901 cgctaatccc gaacaggcgc caaaggctag cgcagaaaaa gcatcaacac cgtcttcaac
     3961 taatataaaa gtaacacaac caacatcaac agcaactaca ccatcttcgt ctaatgtaga
     4021 cacaccacaa cctcaatcga caaacccagc aacaccgcct atagaaatta atcctaaata
     4081 taaagattta agagcatatt atactaaacc aagttttgaa ttcgaaaaac aatttggatt
     4141 tatgctgaaa ccatggacga cggttagatt tatgaatatt attcctaata ggttcatcta
     4201 taaaatagct ttagttggaa aagatgataa aaaatataaa gatggacctt acgataatat
     4261 cgatgtattc atcgtattag aagacaataa atatcaatta aaaaaatatt ctgtcggtgg
     4321 tatcacgaag acgaatagta aaaaagttga tcacaaagca gaattaaaca tcactaaaga
     4381 agataataaa ggaaagattt caagcgatgt ttcagaatat aagattacta aggaagagat
     4441 ttccttgaaa gagcttgatt ttaaattgag aaaacaactt attgaacaac ataatctata
     4501 cggtaacatt ggttcaggaa caatcgttat taaaatgaaa aacggtggga agtatacgtt
     4561 tgaattacac aaaaaactac aagaacatcg catggcagac gtcatagatg gcactaatat
     4621 tgatagcatt gaagtgaata taaaataatc atgatggatt ctaaatagaa actatcagct
     4681 atgaaacaag aagttaagta acaaaggcct cccaaatgtt gcttggcttc ttttattacg
     4741 cacaatgata taagaaaacg aatatactct tgtttgtaaa agtggcattt ctatggctta
     4801 aaagtgacga aacctcaaat atgccaagtg ttgactcaca tcaaaatcag ttttatttaa
     4861 cgaacattat ggatttctta acgatgattc aaataaagtt aaacaaggtt taatgtgaat
     4921 ggaacaatac gccaactata ataaagctgt atgattcaaa gaacgtaatc gaacaaatct
     4981 aataattacg attggagcat acaactatga aaatgacaac aattgctaaa acaagtttag
     5041 cattagggct tttaacaaca ggcgtaatca caacgacaac gcaagcagca aacgcgacaa
     5101 caccatcttc aactaaattg gaaacaccgc aaccaacacc gacgtcaact aaaacagagc
     5161 agtcgcctaa ggctaacgca gaaaatgtac tagctactaa tcccgaacag tcacctaagg
     5221 ctaacgcaga aaaagcgaca acaccggcct caactgatat aaaaacaccg caatcaacac
     5281 caaacgcaac aacaccacct tcaattaaag tggaaacacc acaatctcca aatacaaaac
     5341 aagtaccaac agaaataaac cctaaatata aagatttaag agcgtattat acgaaaccaa
     5401 gtttagaatt taaaaatgag attggtatta ttttaaaaaa atggacgaca ataagattta
     5461 tgaatattgt tccagattat ttcatatata aaatagcttt agttggaaaa gacgataaga
     5521 aatatggtga aggagtgcat aggcatgtcg atgcatttat cgttttagaa gaaaaaaata
     5581 aatatgaagt ggaaatatac tctgtcggtg gtatcacaaa gagtaatagt aaaaaagttg
     5641 atcacaaagc aggaataaga attactaaag aagataataa aggtacaatc tcgcatgatg
     5701 tttcagaata caagattact aaagaagaga tttccttgaa agaacttgat tttaaattga
     5761 gaaaacaact tattgaaaaa cataatctgt acggcaatgt tggttcggga aaaattgtta
     5821 ttaaaatgaa aaacggtgga aagtacacat ttgaattaca caaaaaacta caagaacatc
     5881 gcatggcaga cgtcatagat ggcactaata ttgatagcat tgaagtgaat ataaaataat
     5941 catgacgttc tctaaataga acctgtcatg gataaaacaa gaagttaagc gacaacggcc
     6001 tacatgttgc ttagcttctt ttgctatgtt caataaaatg agaacccgaa tattcgatgg
     6061 ggccaaatat tacatacttg tgtcctgaat gtgttgtgaa atccctatat aactagtgtt
     6121 aaagcacaac gaaatcattt ttatttaacg aacattatag attccttaat ttacttaacg
     6181 atgattcaat gatagttaaa taaggattaa tatgaatggc caaatgcgcc agctataata
     6241 aaactgtatg attcaataga cgtaagcgaa caattctaat aattacgaat ggagcataca
     6301 acaatgaaaa tgacagcaat tgtgaaagca agtttagcat taggtatttt agcaactgga
     6361 acaataacat caacgcatca aactgtaaat gcgagtgaac acgaatcaaa atatgaaaat
     6421 gtgacaaaag atatatttga cttgagagac tactatagtg gtgcaagtaa agaacttaaa
     6481 aatgttatag gttatcacta tagtaaaggc ggtagacact atcttgttat tgataaaaat
     6541 agaaagttca caagagtaca gatatttggt aaagatattg aaagatttaa agcacgcaaa
     6601 aatccgggat tagacatatt tgttgttaaa gaagcggaaa accgtaacgg tacagtttat
     6661 tcatatggtg gcgtgacaaa gaaaaataaa ggcgcctatt atgattattt aaacgcacca
     6721 agatttcaaa ttaaaaaaga cgaaggtgac ggtattgcta cgtacgatag aatacactac
     6781 atttataaag aagaagtatc acttaaagaa ttggatttta aattgagaca gtatttaatt
     6841 caaaattttg gactgtataa aaagtttcct aaagatagca agataaaagt gacaatgaaa
     6901 gatggcggct attatacttt tgaacttaat aaaaaattac aaacaaatcg catgagtgat
     6961 gtcattgacg gtagaaatat tgagaaaata gaagtcgaca ttagataatt caatgaaata
     7021 tggataatag taaaatatgg atagtataga ggagttaggc aacataagtt gcttagcttc
     7081 ttttttgtgt cgttacgatc ggaaatgaag tgggtcgatg aaagataata acgacaataa
     7141 aatttttgaa aatagttgat acctatagtc gcacggtgtc gcattagtga catgaaacaa
     7201 tgtggaaaac ataattaaac tgaaggaaag tgtgaaatgt gaaaaaagct gcgttaagtt
     7261 taaaaaatag attaaagctg ttaggattcc attaattagc ttaacattgg ttcaggaata
     7321 gttaaaatga ggttaattca tagcttagta tcccgcttat ataatgatag tagattgttc
     7381 gtattacgta attgaaataa tcatatgaaa attattataa gcaaatttct aaatagattg
     7441 ggagaatagt actatgaaat taaaaacgat agctaaagca acattagcat taggattatt
     7501 agctactggt gtaataacaa cagaaagtca aacagtaaaa gcggcagaat caactcaagg
     7561 tcaacacaat tataaatcgt taaaatacta ctatagcaag ccaagtatag agttaaaaaa
     7621 tcttgatggt ttgtatagac agaaagtgac agataaagga gtatatgttt ggaagaatcg
     7681 aaaagattat tttgttggct tgcttggtaa agatattgaa aaataccctc aaggtgagca
     7741 tgataagcaa gatgcatttt tagtcatcga ggaggaaact gttaatggaa gacaatattc
     7801 aattggtggt ttaagtaaga caaatagtaa agaatttagt aaagaagtcg atgttaaagt
     7861 aactagaaaa aatgatgaat catcggaaaa gtctaaagat agtaaattta aaattactaa
     7921 agaagaaatc tcattaaaag agttggactt taaattaaga aaaaaattga tggaagaaga
     7981 aaaattatat ggtgctgtca acaatagaaa aggtaaaatt gtagttaaaa tggaagatga
     8041 taagttttat actttcgaac ttacaaaaaa attacaaccg catcgcatgg gtgacacgat
     8101 agatggtact aaaatcaaag aaattaatgt tgagctagaa tataaataat ctttggacaa
     8161 gcagactagt aattataggg aagttaagcg ataacatatt gcttagcttc ttttttgttt
     8221 tgttatgatg aaaaaaggag cgggtcataa tcaaattttt gaaaaaaagg ttgataccta
     8281 tagtcgcgcg ttgtcctttt cgtgacttga aacaatacag aatttgcaat taaattgaag
     8341 gaaaatgtga aatgtgaaaa aaacagcgtt aagttataaa aatagattaa cgctgttaga
     8401 ttttaattaa ctaacttaat gttggttcaa aaatagttaa aacgaggtta attcatagcc
     8461 caacatctca gaaatataat gatagtagat tgttcgtatt acgtaattga attaatcata
     8521 tacaaataaa tttataaaat agattgggag aattctacta tgaaactaaa aacgttagct
     8581 aaagcaacat tagcattagg attattaact acaggcgtta tcacaactga aggtcaagca
     8641 gtccacgcaa aagataagca agaaagagta cagcatttat atgatattaa agacttacat
     8701 cgctactact cagcaccaag ttttgaatac agtaatatta atggcaaagt tgaaaactac
     8761 aatggatcta acgttgtacg ctttgagcaa aaagatcaaa atcaccaatt attcttatta
     8821 ggaaaagata aagaggaata taaagaaggt cttaaaggtc aaaacgtatt tgtagtacaa
     8881 gaattaattg atccaaacgg cagactatct acagttggtg gtgtaacgaa gaaaaataac
     8941 aaatcttctg aaactaatac acatttattt gttaataaag tgtatggcgg aaatttagat
     9001 gcatcaattg actcattttt aattaataaa gaagaagttt cactgaaaga acttgatttc
     9061 aaaattagaa agcaattagt tgaaaaatat ggtttatata aaggtacgac taaatacggt
     9121 aagatcacga tcaatttgaa agacgagaaa aaggaagtaa ttgatttagg tgataaactg
     9181 caattcgagc gcatgggtga tgtgttgaat agtaaggata ttcaaaatat agcagtgact
     9241 attaatcaaa tttaaattaa acaatcaatg actttaaagt aataaatttt gaagcagctt
     9301 agcgatgaaa tgttgaatag atacgtacac cttacaaaaa ggagcgtatt taaaacaacc
     9361 tcatcgttag gctgtttttt tacgttttat aacgcaggtt atgagcatac taaaaattta
     9421 cattgctctt gaaagtgagg cttattagct cttcattaac aatgattcaa gtttaattaa
     9481 acgagtgtta attacagtct gtctcaaggc aatttataat aaaggcatat gattcaaatt
     9541 acgtaatgaa aacaatccaa tacatttaga ttggagcata tgaatatgaa attaacagcg
     9601 atagctaaag caacattagt attaggaata ttaacaacaa gtgtgattac agcagaaagt
     9661 caaacagttc acgcaaaagg aaagtatgaa aaaatgaacc gtttatatga tacaaacaag
     9721 ttacatcaat actattcagg acatagttat gagttaacaa atgttagtgg ccaaagtcaa
     9781 agttattatg actctaacgt tttgcttttt aaccaacaaa atcaaaagtt ccaagtgttt
     9841 ttattgggaa aagatgaaaa taaatacaaa gaaaaaacac atggcctcga tgtctttgcg
     9901 gtaccagaat tagtagattt agatggaaga atatttagtg ttagtggtat aacaaagaaa
     9961 aacgtaaaat caatatttga gtctctaaga acgccaaact tactagttaa aaaaatagac
    10021 gataaagacg gtttttcgat tgatgaattt ttctttattc aaaaggaaga actttcattg
    10081 aaggaacttg attttaaaat aagaaaactg ttgattaaaa aatacaaact gtatgaaggt
    10141 gcagctgata aaggtagaat tgttattaat atgaaagacg aaaataagta tgaaattgat
    10201 ctaagtgata aattagattt cgagcgtatg gcagatgtca ttaatagtga acaaattaaa
    10261 aacatcgaag tgaatttgaa ataataaaat atatagaata aaagcttaag aagcagttta
    10321 ataatcacat gtttaatgat attgatacgt gtttcaataa taaaatacat atcgagcatt
    10381 gacgacgtta ttaagctgct tttttgtaga ctttatagcg aataacttaa gatttaacac
    10441 tagttaaaaa aacaataatc caccaaaaac ataactatgt tgctattaaa aatcagctaa
    10501 tatgaatgtt aaaatgcgtt tgtttttcat taacaatgtt tcaattttat ttaaatgagc
    10561 gttaatgtca gtctgttttg ctgcacttta taataaagat gtattattca aattacgtaa
    10621 tcataacaat ccaatacatt aagattggag cacagaaata tgaaattaac aacgatagct
    10681 aaagtaacat tagcattagg aatattaact acaggtgtgt ttacaacaga aagtcaaact
    10741 gttcacgcga aagtagaact ggatgaaaca caacgcaaat attatatcaa tactattctg
    10801 aagaaagttt tgaaccaaca aacattagtg ttaaaagtga agattattat ggctcaaatg
    10861 ttttaaactt taaccaacga aataaaacct tcaaagtatt tttaattgga gactataaaa
    10921 ataaatataa agagaaagca catggccttg atgtctttgc agtacctgaa ttaattgata
    10981 caaaaggtcg catatatagt gttggcggta taacaaagaa aaatgtgaga tcagtgtttg
    11041 gatttgtaag taatccaagt ctacaagtta aaaaagttga tgctaaaaat ggattttcga
    11101 taaacgagtt gttctttatt caaaaggaag aagtatcatt gaaagaactt gattttaaaa
    11161 taagaaaact attaattgaa aaatatagat tgtacaaagg aacggcagat aaaggtagaa
    11221 ttgttatcaa tatgaaagac gaaaagaaat atgaaattga tttaagtgaa aaattaagtt
    11281 ttgatcgtat gtttgatgtc atggacagta agcaaattaa aaacattgaa gtgaatttaa
    11341 attagttcga gttaatagca taatagctta agaagcgact taacgacatg tgaattgaca
    11401 tccgtgtcct tatataagga actgtgttaa atacattact gttgttaagt tgtttttgca
    11461 ttttaaagag cagaacagag taacaaagtc agatatagta aaatccagta aaataactaa
    11521 atgaaataat aaaagccatt taacctgaac attaaaatat atttgttttt cattaacaat
    11581 gtttcaagat tatttaaatc aaggttaatt ctagtctgct gagatgccct ttataataaa
    11641 aatgtatgat tcaaattacg taatgaaaac aatctaatac atttagattg gagcaaaaaa
    11701 atatgaaatt aacagcgata gctaaagcaa cattagcttt aggaatttta acaacaggaa
    11761 ctttaataac agaggtccat tcaggtcatg caaaacaaaa tcaaaagtca gtaaataaac
    11821 atgacaagga ggcactacac cgatactaca ctggaaactt taaggaaatg aaaaacatta
    11881 atgctttgaa acatggtaaa aataacttac gttttaaata tagaggtatg aagactcaag
    11941 tattattgcc tggaaatgaa taccgtaaat atcaacagcg aagatatacg gggttagatg
    12001 tgttttttgt tcaagaaaga agagacaagc acgacatatc atatactgtt ggtggcgtta
    12061 caaagacgaa taaaacatct ggctttgtca gtgaaccaag attaaatgtt acaaaagaaa
    12121 atggtaaaga tgcttttgtg aaaggttacc cttatcatat taataaagaa gaaatatcac
    12181 tgaaagagtt agattttaag ttgagaaagc atctaatcga aaaatatgga ctttataaaa
    12241 caatttcaaa agagggtagg gttaaaatta gcttgaaaga tggcagtttt tataaccttg
    12301 atttaagata caaattagat ttcaaatata tgggggaagt catagatagt aaacaaatta
    12361 aagatattga agtgaattta aagtaaat
//
LOCUS       DAHHRR010000076        12273 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.076, whole genome shotgun sequence.
ACCESSION   DAHHRR010000076 DAHHRR010000000
VERSION     DAHHRR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12273
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.076"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            335..1177
                     /locus_tag="NBI42_001798"
     CDS             335..1177
                     /locus_tag="NBI42_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1778211.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVPGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDDGENIDAYILRETL
                     VDNN"
     gene            complement(1302..1613)
                     /locus_tag="NBI42_001799"
     CDS             complement(1302..1613)
                     /locus_tag="NBI42_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD1778212.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            1700..2776
                     /locus_tag="NBI42_001800"
     CDS             1700..2776
                     /locus_tag="NBI42_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD1778213.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTSFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPAFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            2841..3152
                     /locus_tag="NBI42_001801"
     CDS             2841..3152
                     /locus_tag="NBI42_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1778214.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            3252..4109
                     /locus_tag="NBI42_001802"
     CDS             3252..4109
                     /locus_tag="NBI42_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HCD1778215.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            4138..4734
                     /locus_tag="NBI42_001803"
     CDS             4138..4734
                     /locus_tag="NBI42_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCD1778216.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEETGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            4755..8579
                     /locus_tag="NBI42_001804"
     CDS             4755..8579
                     /locus_tag="NBI42_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCD1778217.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKSKAHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTEASQLHTNGIENEAVSNDENKQPSIQNEDTDDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSDVTSQRQSALQHNVEVNNQDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEATTSNSEDNTNRDASEMHVDAPKTQEH
                     VVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDGHVLEDSSMNEIEK
                     QNAEITENVQNEAAESEQNVEEKTIENINPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSESKTESQATPSSSTDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQHYSEQKPSADSIQTEIFEESQDDNQLENEQVDQSTPSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQPSFSNQNEDTANENRPRTNQPDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIEPDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDHLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            8603..9916
                     /locus_tag="NBI42_001805"
     CDS             8603..9916
                     /locus_tag="NBI42_001805"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCD1778218.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVVLFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            9990..10481
                     /locus_tag="NBI42_001806"
     CDS             9990..10481
                     /locus_tag="NBI42_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCD1778219.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            10555..11919
                     /locus_tag="NBI42_001807"
     CDS             10555..11919
                     /locus_tag="NBI42_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778220.1"
                     /translation="MTQQQNNKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAETDKSAAVSNEESKAAALKAQQVAI
                     KEEASANNLSDTSQEAQEIQEAKREAQAETNKSAEVSNEESKAVALKAQQAAIKEEAS
                     ANNLSDTSQEAQEIQEAKKEAQAEKDKSVAVSNEEPKASTLNVSKEESQAERLANAAK
                     QKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNTDNKDTVK
                     QKEAKFENGVITRKSDEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQ
                     HNKKSAQGAKKQSSSSKSTQKNNQTSNKNSKTTKAKSSNASKTSNAKVEKAKSKIEKR
                     TFND"
     gene            12075..12158
                     /locus_tag="NBI42_001808"
     CDS             12075..12158
                     /locus_tag="NBI42_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778221.1"
                     /translation="MLFVTSLEYNSDIAHFKLKMEKYSIIK"
BASE COUNT         4782 a         1691 c         2281 g         3519 t
ORIGIN      
        1 ttgaatataa aaaaagtgca attatctcta taattaaaag tgactaaaca aataactaaa
       61 ggagataatg cgccagtgaa cgattatata ctaaatatct ttgatttaaa agaataaagc
      121 ttgcatttta cttggaaatt ttaattatta atttaaaatt acagtccgat ggttatgatg
      181 catttacata taatataatt gcattaatct ttataagtag ttggaagtgc attagtgtgg
      241 ggcgtttcgt ttttgttcgc atttatgaat agtgatatga tgtaataaac acatgctaat
      301 tgtaataacg aatacagaaa gaataggtga tgttatgaaa atcggggata tatctattca
      361 ttatctaaat ggcggcaata caaaaatgga tggcggtgca atgtttggtg ttgttccgaa
      421 gccgttgtgg tcaaagcaat acaatgcaaa tgaacgaaat caaatcaatt taccgacaca
      481 tccaattttg attcaaacgg ctcaatataa tttgattata gatgcgggta ttggtaatgg
      541 taaattatct gaaaagcaat tacgtaattt tggagtagat gaagaaagtc atataattgc
      601 tgatttggca aattataatt taacgccaaa ggatattgat tatgtgctaa tgacacatat
      661 gcattttgat catgctgccg gtttgactga tcaagcggga catgcaattt ttgaaaatgc
      721 gattcatgtt gtgcaacaag atgagtggca tgagtttatt gcacctaata taagaagtaa
      781 atcaacttac tgggataaga ataaaggcga ttatagtaac aagttgattt tattcgaaaa
      841 acattttgaa ccggttccgg gtatcaagat gcaacatagt ggaggtcata gctttggcca
      901 cacaatcatt acgattgaaa gtcaaggaga taaagcagtt catatgggtg atatattccc
      961 gactactgca cataaaaatc ctctatgggt aacggcatat gatgattatc ctatgcaatc
     1021 gattcgtgaa aaggaacgca tgataccata ttttatccag caacaatatt ggttcttgtt
     1081 ttatcatgat gaaaactact ttgctgtaaa atacagcgat gatggtgaaa acatagatgc
     1141 atatatttta cgtgaaacat tagttgataa taactaaaat aaagatgtat tactaaacaa
     1201 attttcaaga ataaaaaatt gagccacatc caatcttact aattagggtg tggctcattt
     1261 ttaagtttta cgatccaaat caaatatgga taaaattcgt attaacgctc tacaatgtta
     1321 atgacttcac cagtatatgc atctgcataa aaatcataat gaatattttg accattttta
     1381 atagttgtaa ttccaccttg ataaactaaa cggtatttat cagtttcagg atgaacgaat
     1441 ggttcataaa cgatatatga gcctttgaca ttcataaaat atgattttac ttcattaagt
     1501 actttaacgg gattatagcg tttacgatta ataaattgga tgattgaaat ggtagaaata
     1561 attacaatgg ctactgcaat tattgttgga attatatatt tcagtttagt catcatttat
     1621 gctcccttga tttcaaattc atatcattag tttaccatat tgaagatgat ataataactt
     1681 ataaggagtg agaactttta tgaacataaa taaaaaagta acattacaac gaattcaaac
     1741 tttaactgag cttcatgggg caccaggttt tgaagaagaa gtaaagaatt atatgactca
     1801 gcaaatggcg ccgtacgtag atgaatttat tgaaaatcgt atgggtggtt tttttggtgt
     1861 gaaaaaatct aaaaatccaa atgcaaaacg tgtaatgatt gcagcacata tggatgaaat
     1921 tggatttatg attacaaaca tcactaaaaa tggaatgatt caattcacaa atttaggtgg
     1981 tgttgcaaat gatatttggc aaggacaacg cttagtaatt aaaaatagaa atggcgataa
     2041 aattatcggt gttgtttcta atatacctaa acattttcgt actggtagtg aaggtgcacc
     2101 ggaaattaaa gatttaacat tagatatagg tgctcaaaat gaagatgaag tgcgtgagcg
     2161 cggaatagat ataggagata caattgtacc tcacacgtca ttcacacagt tatctgaaca
     2221 tcgttatagt gctaaagcat gggataatcg ttatggttgt gtcttggcaa ttgaaatact
     2281 agaattatta aaagatatag aattagatgt agacttgtat gttggcgcaa atgttcaaga
     2341 agaggttgga ttacggggtg cgaaagcatc tgcagagatg atagacccag acgttgcatt
     2401 tgtagtcgat tgttcacctg ccaatgacgt taaaggtaat caaccattat ccggtgaact
     2461 tggtaaaggt acgttaattc gcataaaaga cggtacaatg attttaaagc ctgcatttag
     2521 agactattta ttaaagttag tagaagcaca tgacattgaa catcaatact atatgtcacc
     2581 tggtggaaca gatggtggag aaattcataa agctaatatt ggtattccga ctgcagttat
     2641 tggtgtatgt gcacgatata ttcatagtac agactcagta tttgatataa gagactattt
     2701 tgcagctaga tctttacttt cagaagccat ttgtaattta gacaataatc aaatagaaac
     2761 attacaatat aaataatcgg gtaataacaa ttattatctc taaatagtta tatataatca
     2821 ttaattaagg agacataaaa atgaaacaac ttgaatcaga acaacaattt gaatctttaa
     2881 aacaaggtgc tacagtattt gaattcactg caggctggtg tccagattgt agagtgatag
     2941 aaccagattt accggaatta gaagcgagat atcctatgtt tgacttcgta tcagtagatc
     3001 gtgataaatt tatggatatt tgtattgaaa atggtattat ggggattcca agttttctag
     3061 tatataaaaa tggagaactg cttggaagtt atattggaaa agaacgaaaa tcaattgaac
     3121 agatagatgc atttttagct caatacgtgt aatttagact agagaaaaac ggggtaatac
     3181 tcgtttttct ctgttactat gtgttgattt attgtaaact attataaggt gcgaaattag
     3241 gagtgttaca tatgaatacc tttcaaatga gagataaatt aaaggaacgt ttaagccatt
     3301 tagacgttga ttttaaattt aatcgtgaag aagaaacttt gcgtatttat cgaacagata
     3361 ataacaaagg tatcacgatt aaacttaacg ctatagtcgc aaaatatgaa gataaaaaag
     3421 aaaaaattgt agatgaaatt gtttattacg ttgatgaagc tattgcacaa atggcagata
     3481 aaacccttga gagtatatca tctagtcaaa ttatgcctgt cattagagcg actagctttg
     3541 ataaaaaaac taaacaaggt gttcctttta tctatgatga gcatactgca gaaacagcag
     3601 tttattatgc agtcgattta ggaaaatcat atcgtctaat tgatgaaagc atgttagagg
     3661 atttaaagtt aactgaacaa caaataagag aaatgtctct gtttaatgtt agaaaattgt
     3721 caaattcata tacgactgat gaagtaaaag gtaatatttt ttattttatt aactcaaatg
     3781 acgggtatga tgcaagtagg atactaaata ctgcattttt aaatgaaatt gaggcacaat
     3841 gtcaaggcga aatgctcgta gcagtgccac accaagatgt gttaattatt gcagatatac
     3901 gcaataaaac aggatatgat gtgatggcac atttaacaat ggaatttttc actaaaggtc
     3961 tagttccaat tacatcatta tcctttggat ataaacaggg tcatcttgaa ccgatattta
     4021 ttttaggtaa aaataataaa caaaaaagag atccaaacgt gattcagcgt ttagaagcaa
     4081 atcgtcgtaa atttaataaa gataaataga aataattgga taaggagttt tgtcataatg
     4141 aatttatttt acaatcctaa atatgtagga gatgtcgcat ttttacaaat tgaaccagtt
     4201 gaaggtgaat taaactacaa taaaaaaggt aacgttgttg aaattactaa tgaaggtaat
     4261 gttgtaggtt ataatatttt tgaaatttca aaagatataa caattgaaga aacaggtcat
     4321 attaaattaa ctgatgaact tgtaaatgta ttccaaaagc gtatttcaga agctggtttt
     4381 gattataaat taaatgctga tctatcaccg aaatttgtag ttggctacgt tgaaactaaa
     4441 gacaaacatc ctgatgcaga taaattaagt gtactaaatg taaacgttgg aaatgacaca
     4501 ttacaaattg tatgtggcgc gcctaacgtt gaagctggac agaaagttgt tgttgctaaa
     4561 gtaggtgcag tgatgcctag cggtatggta attaaagatg ctgaattacg tggtgttgcc
     4621 tcaagcggta tgatttgttc aatgaaagaa ttgaatttac ctaatgcacc tgaagaaaaa
     4681 ggtattatgg tattaaatga cagctatgaa attggacaag cattttttga ataattaagg
     4741 aaggtagtga aaatatgagc tggtttgata aattattcgg cgaagataat gattcaaatg
     4801 atgacttgat tcatagaaag aaaaaaagac gtcaagaatc acaaaatata gataacgatc
     4861 atgactcatt actgcctcaa aataatgata tttatagtcg tccgaggggg aaattccgtt
     4921 ttcctatgag cgtagcttat gaaaatgaaa atgttgaaca atctgcagat actatttcag
     4981 atgaaaaaga acaataccat cgagactatc gcaaacaaag ccacgattct cgttcacaaa
     5041 aacgacatcg ccgtagaaga aatcaaacaa ctgaagaaca aaattatagt gaacaacgtg
     5101 ggaattctaa aatatcacag caaagtataa aatataaaga tcattcacat taccatacga
     5161 ataagccagg tacatatgtt tctgcaatta atggtattga gaaggaaacg cacaagtcaa
     5221 aagcacacaa tatgtattct aataatacaa atcatcgtgc taaagattca actccagatt
     5281 atcacaaaga aagtttcaag acttcagagg taccgtcagc tatttttggc acaatgaaac
     5341 ctaaaaagtt agaaaatggt cgtatccctg taagtaagcc ttcagaaaaa gttgagtcag
     5401 ataaacaaaa atatgataaa tatgtagcta agacgcaaac gtctcaaaat aaacaattag
     5461 aacaagagaa acaaaatgat agtgttgtca aacaaggaac tgcatctaaa tcatctgatg
     5521 aaaatgtatc atcaacaaca aaatcaatgc ctaattattc aaaagttgat aatactatca
     5581 aaatagaaaa tatttatgct tcacaaattg ttgaagaaat tagacgtgaa cgagaacgta
     5641 aagtgcttca aaagcgtcga tttaaaaaag cgttgcaaca aaagcgtgaa gaacataaaa
     5701 acgaagagca agatgcaata caacgtgcaa ttgatgaaat gtatgctaaa caagcggaac
     5761 gctatgttgg tgatagttca ttaaatgatg atagtgactt aacagataat agtacagagg
     5821 ctagtcagct tcatacaaat ggcatagaga atgaagctgt atcaaatgat gaaaataaac
     5881 aaccgtcaat acaaaatgaa gacactgatg acactcatgt agatgaaagt ccatacaatt
     5941 atgaggaagt tagtttgaat caagtatcga caacaaaaca attgtcagat gatgaagtta
     6001 cggtttcgga tgtaacgtct caacgtcaat cagcactgca acataacgtt gaagtaaata
     6061 atcaagatga actaaaaaat caatccagat taattgctga ttcagaagaa gatggagcaa
     6121 cgaataaaga agaatattca ggaagtcaaa tcgatgatgc agaattttat gaattaaatg
     6181 atacagaagt agatgaggct actacttcaa atagcgaaga taataccaat agagacgcgt
     6241 ctgaaatgca tgtagacgct cctaaaacgc aagagcacgt agtaactgaa tctcaagtta
     6301 ataatatcga taaaacggtt gataatgaaa ttgaattagc gccgcgtcat aaaaaagatg
     6361 accaaacaaa cttaagtgtc aactcattga aaacgaatga tgtgaatgat ggtcatgttc
     6421 ttgaagattc aagcatgaat gaaatagaaa agcaaaacgc agaaattaca gaaaatgtgc
     6481 aaaacgaagc agctgaaagt gaacaaaatg ttgaagagaa aactattgaa aacataaatc
     6541 caaagaaaca gactgaaaag gtttcaactt taagtaaaag accatttaat gttgtcatga
     6601 cgccatctga taaaaagcgt atgatggatc gtaaaaagca ttcaaaagtc aatgtgcctg
     6661 aattaaagcc tgtacaaagt aagcaagctg tgagtgaaag caagactgag agtcaagcca
     6721 caccatcatc aagcactgat tcacaagagt cgaacacaaa tgcatataaa acaaataata
     6781 tgacatcaaa caatgttgag aacaatcaac taattggtca tgcagaaaca gaaaatgatt
     6841 atcaaaatgc acaacattat tcagagcaga aaccttctgc tgattcaatt caaacagaaa
     6901 tatttgaaga aagccaagat gataatcaat tggaaaatga gcaagttgat caatcaactc
     6961 cgtcttcagt ttcagaagta agcgacataa ctgaagaaag cgaagaaaca acacatccaa
     7021 acaatactag tggacaacaa gataatgatg atcaacaaaa agatttacag ccttcatttt
     7081 caaatcaaaa tgaagataca gctaatgaaa atagacctcg gacgaaccaa ccagatgttg
     7141 caacaaatca agctgtacaa acatctaagc cgatgattcg taaaggccca aatattaaat
     7201 tgccaagtgt ttcattacta gaagaaccac aagttattga gccggatgag gactggatta
     7261 cagataaaaa gaaagaactt aatgacgcat tattttactt taatgtacct gcagaagtac
     7321 aagatgtaac tgaaggtcca agtgttacaa gatttgaatt atcagttgaa aaaggtgtta
     7381 aagtttcaag aattacggca ttacaagatg acattaaaat ggcattggca gcgaaagata
     7441 ttcgtataga agcgccaatt ccaggaacta gtcgtgttgg tattgaagtt ccgaaccaaa
     7501 atccaactac agtcaactta cgttctatta ttgaatctcc aagttttaaa aatgctgaat
     7561 ctaaattaac ggttgcgatg gggtatagaa ttaataatga accattactt atggatattg
     7621 ctaaaacgcc acacgcacta attgcaggtg caactggatc agggaaatca gtttgtatca
     7681 atagtatttt gatgtcttta ctatataaaa atcatcctga ggaattaaga ttattactta
     7741 ttgatccaaa aatggttgaa ttagctcctt ataatggttt gccacattta gttgcaccgg
     7801 taattacaga tgtcaaagca gctacacaga gtttaaaatg ggccgtagaa gaaatggaaa
     7861 gacgttataa gttatttgca cattaccacg tacgtaatat aacagcattt aacaaaaaag
     7921 caccatatga tgaaagaatg ccaaaaattg tcatagtaat tgatgagttg gctgatttaa
     7981 tgatgatggc tccgcaagaa gttgagcagt ctattgctag aattgctcaa aaagcgagag
     8041 catgtggtat tcatatgtta gtagctacgc aaagaccatc tgtcaatgta attacaggtt
     8101 taattaaagc caacatacca acaagaattg catttatggt atcatcaagt gtagattcga
     8161 gaacgatatt agacagtggt ggagcagaac gcttgttagg atatggcgat atgttatatc
     8221 ttggtagcgg tatgaataaa ccgattagag ttcaaggtac atttgtttct gatgacgaaa
     8281 ttgatgatgt tgttgatttt atcaaacaac aaagagaacc ggactatcta tttgaagaaa
     8341 aagaattgtt gaaaaaaaca caaacacaat cacaagatga attatttgat gatgtttgtg
     8401 catttatggt taatgaagga catatttcaa catcattaat ccaaagacat ttccaaattg
     8461 gctataatag agcagcaaga attatcgatc atttagagca actcggttat gtttcgagtg
     8521 ctaatggttc aaaaccaagg gatgtttatg ttacggaagc agatttaaat aaagaataat
     8581 tatgagtaag gagttttata taatgacaca ctatcatttt gtcggaatta aaggttctgg
     8641 catgagttca ttagcacaaa tcatgcatga tttaggacat gaagttcaag gatcggatat
     8701 tgagaactac gtatttacag aagttgctct tagaaataag gggataaaaa tattaccatt
     8761 tgatgctaat aacataaaag aagatatggt agttatacaa ggtaatgcat tcgcgagtag
     8821 ccatgaagaa atagtacgtg cacatcaatt gaaattagat gttgtaagtt ataatgattt
     8881 tttaggacag attattgatc aatatacttc agtagctgta actggtgcac atggtaaaac
     8941 ttctacaaca ggtttattat cacatgttat gaatggtgat aaaaagactt catttttaat
     9001 tggtgatggc acaggtatgg gattgcctga aagtgattat ttcgcttttg aggcatgtga
     9061 atatagacgt cactttttaa gttataaacc tgattacgca attatgacaa atattgattt
     9121 cgatcatcct gattatttca aagatattaa tgatgttttt gatgcattcc aagaaatggc
     9181 acataatgtt aaaaaaggta ttattgcttg gggtgatgat gaacatctac gtaaaattga
     9241 agcagatgtt ccaatttatt actatggatt taaagattcg gatgacattt atgctcaaaa
     9301 tattcaaatt acggataaag gtactgcttt tgatgtgtat gtggatggtg agttttatga
     9361 tcacttcctg tctccacaat atggtgacca tacagtttta aatgcattag ctgtaattgc
     9421 gattagttat ttagagaagc tagatgttac aaatattaaa gaagcattag aaacgtttgg
     9481 tggtgttaaa cgtcgtttca atgaaactac aattgcaaat caagttattg tagatgatta
     9541 tgcacaccat ccaagagaaa ttagtgctac aattgaaaca gcacgaaaaa aatatccaca
     9601 taaagaagtt gttgcagtat ttcaaccaca cactttctct agaacacaag catttttaaa
     9661 tgaatttgca gaaagtttaa gtaaagcgga tcgtgtattc ttatgtgaaa tttttggatc
     9721 aattagagaa aatactggcg cattaacgat acaagattta attgataaaa ttgaaggtgc
     9781 atcgttaatt aatgaagatt ctattaatgt attagaacaa tttgataatg ctgttgtttt
     9841 atttatgggt gcaggtgata ttcaaaaatt acaaaatgca tatttagata aattaggcat
     9901 gaaaaatgcg ttttaatatg tttataatag agtagtatgg gtatttatta ttaatgacat
     9961 tattacatgt taattaggag gcgtttttaa tggattggat tttaccaatt gctggaatta
    10021 tcgctgcgat tgcattctta attttatgta tcggtatcgt agctgtatta aattctgtta
    10081 agaaaaactt agattatgtt gcaaaaacac ttgatggtgt agaaggtcaa gttcaaggta
    10141 ttactcgtga aacaacagat ttacttcata aagtaaaccg tttaactgag gatatccaag
    10201 gtaaagtaga tcgtttaaac tcagttgtag atgctgttaa aggtatcggt gactcagtac
    10261 aaacgttaaa cagctctgta gatcgtgtaa caaattcaat tacacataat atttctcaaa
    10321 atgaagataa aatctcacaa gttgttcaat ggtcaaatgt tgcaatggaa attgcagaca
    10381 aatggcaaaa tagacactac cgtcgtggaa gtgcaaatta caaagctaat aatgtagcaa
    10441 ctgatgcaaa tcatagctat acttctcgag tagataaata attaataatt aaacaaatgc
    10501 acttaaatga ggttgttact tatttaagtg catttgttat aggaggatat acaaatgacg
    10561 cagcaacaaa ataataaaag aacattaaaa aataaacaca cttatcaaaa tgaaccacta
    10621 ccaaaccgta aagattttgt tgttagtttt ataactggcg cgcttgttgg ttcagcttta
    10681 ggcttgtatt ttaaaaataa agtttatcaa aaagcagatg atttaaaagt caaagaacaa
    10741 gaactgtcgc aaaagtttga agaaagaaaa acgcaacttg aagaaacggt tgcctataca
    10801 aaagaacgtg ttgaaggatt tttaaacaaa tctaaaaatg agcaagcggc attaaaagca
    10861 caacaagcag cgataaaaga agaagcaagt gcaaataatt tgagtgacac gtcacaagag
    10921 gcacaagaga ttcaagaagc taaaagagaa gcacaagcag aaacagataa aagtgcagct
    10981 gtatcaaatg aagagtcaaa agcagcggca ttaaaggcac aacaagtagc gataaaagaa
    11041 gaagcaagtg caaataattt aagtgataca tcacaagagg cacaagagat tcaagaagct
    11101 aaaagagaag cacaagcaga aacaaataaa agtgcagaag tatcaaatga agaatcaaaa
    11161 gcagtggcat tgaaagcaca acaagcagcg ataaaagaag aagcaagtgc aaataattta
    11221 agtgatacat cacaagaggc acaagagatt caagaagcta agaaagaagc acaagcagag
    11281 aaagataaaa gtgtagctgt atcaaatgaa gaaccaaaag catcgacatt aaacgtatcg
    11341 aaagaagagt cacaagctga aagattagca aacgctgcaa aacagaagca agctaaatta
    11401 acaccaggtt caaaagagag tcaattaact gaagcgttat ttgcagaaaa accagttgct
    11461 aaaaatgact tgaaagaaat tcctcaatta gttactaaaa agaatgacgt atcagagaca
    11521 gagacagtta atacagataa taaagacact gttaaacaaa aagaagctaa atttgaaaat
    11581 ggtgttatta cacgtaaatc tgatgaaaaa acaactaata atacagctgt tgacaagaaa
    11641 tcaggtaaac aatctaaaaa aacaacacct tcaaataaac gaaatgcatc aaaagcatct
    11701 acaaataaaa cttcaggtca gaaaaagcaa cataataaga aatcagcaca aggtgctaag
    11761 aaacaaagta gttcaagtaa gtcaactcaa aagaataatc aaactagtaa taagaattca
    11821 aaaacaacaa aagctaagtc atccaatgca tctaaaacgt caaatgctaa agttgaaaaa
    11881 gctaaaagta aaatagagaa acgtacattc aatgactaag attactaaaa tcgtttaata
    11941 atcaatcata gcagtaagct atgtattata tttttaaaaa ttgaatttca aaataaaagg
    12001 taggcattca ggtgcttacc ttttttgata tcatcagttt attttgaaag gcaacgcgat
    12061 cattccatgt atttatgttg tttgtaacat cgttagaata caatagtgat attgcacatt
    12121 ttaagctgaa gatggaaaaa tattcgataa tcaaataaac aatgaatctt agaaggtaca
    12181 atgatgttca ctaattcaaa tatagacgaa tatgttggtg agtgatgttc actatagatt
    12241 tatattaata tacaaaggtt tttaattttt att
//
LOCUS       DAHHRR010000077        12241 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.077, whole genome shotgun sequence.
ACCESSION   DAHHRR010000077 DAHHRR010000000
VERSION     DAHHRR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12241
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.077"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(24..1115)
                     /locus_tag="NBI42_001809"
     CDS             complement(24..1115)
                     /locus_tag="NBI42_001809"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCD1778222.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKFLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECSLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANMLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNVQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAEA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            1280..2311
                     /locus_tag="NBI42_001810"
     CDS             1280..2311
                     /locus_tag="NBI42_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD1778223.1"
                     /translation="MEPILEMIKTLTDINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGD
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            2804..4210
                     /locus_tag="NBI42_001811"
     CDS             2804..4210
                     /locus_tag="NBI42_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCD1778224.1"
                     /translation="MDVFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSIVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVNKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHANQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEITGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            4207..4773
                     /locus_tag="NBI42_001812"
     CDS             4207..4773
                     /locus_tag="NBI42_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCD1778225.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDNNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQDKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEALKITGRTEDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKKG"
     gene            4779..5777
                     /gene="trpD"
                     /locus_tag="NBI42_001813"
     CDS             4779..5777
                     /gene="trpD"
                     /locus_tag="NBI42_001813"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HCD1778226.1"
                     /translation="MTLLTRIKTETILLESDIKDLIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAITLNI
                     LNGKDQSSRRDVVVLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALKKYHQMRGE"
     gene            5779..6561
                     /gene="trpC"
                     /locus_tag="NBI42_001814"
     CDS             5779..6561
                     /gene="trpC"
                     /locus_tag="NBI42_001814"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCD1778227.1"
                     /translation="MTILAEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYEKYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIDVNNRDLKRFVTNVEHTNTILENKKPNHYYISESGIH
                     NASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            6561..7193
                     /locus_tag="NBI42_001815"
     CDS             6561..7193
                     /locus_tag="NBI42_001815"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCD1778228.1"
                     /translation="MKLKFCGFTSIEDVTATSQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPEHIDKVCVVVNPDLTTIEHVLSNTPINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRENE"
     gene            7186..8401
                     /gene="trpB"
                     /locus_tag="NBI42_001816"
                     /pseudo
     CDS             7186..8401
                     /gene="trpB"
                     /locus_tag="NBI42_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
     gene            8394..9122
                     /locus_tag="NBI42_001817"
     CDS             8394..9122
                     /locus_tag="NBI42_001817"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCD1778229.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFEQLEKHGNQIKCQYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPFELSQRLKQQFSHYGVKIISLVAMTTDDKRIKEIVSHAEGFIYTVTM
                     NATTGQNGAFRPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKANTREEIIKYLQSIQHALNN"
     gene            9444..10706
                     /gene="femA"
                     /locus_tag="NBI42_001818"
     CDS             9444..10706
                     /gene="femA"
                     /locus_tag="NBI42_001818"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HCD1778230.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKNRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANAQKIEEGKRLKEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            10725..11984
                     /gene="femB"
                     /locus_tag="NBI42_001819"
     CDS             10725..11984
                     /gene="femB"
                     /locus_tag="NBI42_001819"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HCD1778231.1"
                     /translation="MKFTELTVTEYDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELCKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
BASE COUNT         4495 a         1763 c         2241 g         3742 t
ORIGIN      
        1 atttatcaaa agagacttaa taattattgg atataacaat caaaatcact caatgcttgc
       61 ataccgcgtt ctcggtcagt aggatttttg aaactaattt ttaaagcacc gtatatatct
      121 tcgcgtactt ctaagattct taagttgctt atagatatat tatgtaaact caggatataa
      181 gtcactttgc ttatcatacc tgattcatcc ggaatatcta catatagatc atacgcagta
      241 tttagtccac ctagttgttt agcgggcagt gcgtcacgat acgatttagc ttcggcaaaa
      301 aatgataaca atttttcaga atcattgctt tcaattagtc tttctaaatc ttgaaactga
      361 ctttttagct gtcgaatcat ttctaaaata tacgttttat tactcaaggt gatatctttc
      421 cacatttgta cattactact agctatacga gtgatatcac gaaaaccacc agctgcaagt
      481 ttattgacta aatgatgttc ttgaccgttc ttttgactaa catgaactaa actagatgca
      541 acgatatgag gtaaatgact tacgacgctt gttacgtagt cgtgttcttc agcagtagtt
      601 acaataaatt tagcaagagt aggtgataac agttctttta acatgtttgc tgcttgctca
      661 tttcttggct cattgtagac taaaatataa taagcgtttt caaataagtg ctttttagca
      721 tttagtacac cagatttatg actaccagcc attggatgac cactgactaa atgaatatta
      781 tgctttaata aactgcattc gtgttgctgt atcattgctt tagtactacc agtatcagaa
      841 acaataacac caggtttagt tggcatatct ataagctcgc taagatattt atttgtgata
      901 gcaacaggtg ttgcataaat aattacatcg gcttttttaa tagcttcact ataatttaaa
      961 aatttttcat taataatacc gattgattta gctttatcta actgagaagt atctgcatcg
     1021 tatgcaataa tattagtatt agggttatgg tattttatat tgctagcaag acttccacca
     1081 attaatccaa gcccaacaaa taaaactgtt gtcatataaa tcaccttatt tcgaaatttt
     1141 cagaataata acattgtaaa tgagctgttg acacagtgca atagtaaata aaaatcgata
     1201 atagcattaa tagataaacg gatataaatc atctacaata aagagtatag taacacaatg
     1261 gcaacggagg ggtaaatcaa tggaaccaat attagaaatg attaaaacat taacagatat
     1321 taatagtcct tcaggagaca cagaagaagc aattcaattt gtcgaaaaat atgcaaaaga
     1381 cttaggttat caaacaacac taacaaataa aggtgcgtta ttaataacag tgccaggcaa
     1441 aaatgatgaa gtacaacgct gtattactgc tcatgttgat actttaggtg caatggttaa
     1501 agaaattaaa gaagatgggc gcttagcaat agaattaatt ggaggattca cgtataacgc
     1561 gattgagggt gaatattgcc aaattaaaac tgatgctggt caaatatata caggaaccat
     1621 ttgtctgcat gaaacaagtg ttcatgtata tagaaataat catgaaatac ctagagacca
     1681 aaagcatatg gaaataagaa ttgatgaagt aactacatca gaagaagata caaagagttt
     1741 aggtatttca gtaggtgatt ttgttagctt tgatccacgt acagttatca cgtcatcagg
     1801 ttttattaaa tctcgtcatt tagatgataa agctagcgta gcgatgatac tacaattact
     1861 aaagaaatta aaagaagagc aaataatatt accacataca acacaatttt atatttctaa
     1921 taacgaagaa ataggttacg gtgcaaatgc atcaattgat tcgaaaatca aagaatatat
     1981 tgcattagat atgggcgcgt tgggagacgg tcaagcatcc gatgaatata cagtttctat
     2041 ttgtgccaaa gatgcttcag gtccatatca taagcaattg aaatcacacc tagttaatct
     2101 ttgcaaaata aataatattc catataaagt agacatatat ccatattatg gttcagatgc
     2161 ttcagcagct ttacatgctg gtgcggatat cagacatggt ttatttggcg ctggcattga
     2221 atcatctcat gcaatggaac gaacacatat tgattctatt aaagcgacag agaaattact
     2281 atacgcatat tgcttatcac caattgagta aacaattagt gttgacaaat atgaacgacc
     2341 tatgtaatat aatgaactat aaaaataatt agaattttct aaagaaatag tagcagatat
     2401 gaaacgtagc aaatagaaag ctaatgggtg atgggaatta gcacgccata tcttgtgaat
     2461 tggactttgg aaaacaattg aatgagtttt gaaagtgaac atgaattatg ttaactaagg
     2521 tggcaccacg gtaacgcgtc cttacaggtt tatgcgttat gtggtgtctt tttatttaga
     2581 caaaatgtag tagttagtta aaggtagcaa cagaaagtta gtgggtgatg tgaactaaca
     2641 ccgagattaa tgaaattggg ttttgtctgc aacagaaaaa ttaaatatag taaagagtga
     2701 actatgaata tttcgaatat tcggttaatt taggtggtac cacgcgtcag cgtcctttat
     2761 attgataagg atgctgacgt ttttttagaa aggagcgtaa agaatggatg tattctataa
     2821 aaaaataaaa gcaaatgtaa cacctgaagt tttagcacaa cttcattcca agaagatcat
     2881 tttggaaagt acaaatcaac aacaaactaa gggtcgctat tctatagtga tatttgatat
     2941 ctacggtact ttaactttag ataacgatgt attatcagta agcactttaa aagagtcgta
     3001 tcaaatcact gaaagaccgt atcattattt aacgactaaa ataaatgaag actatcataa
     3061 tattcaagat gagcaactta agtcattacc gtttatatct ggatatgttg ggacgtgtag
     3121 ctttgattta gtaagacatg aatttcctaa attgcaatca atacaattag aagatcacaa
     3181 gcagcacgat gtacggttat atatggttga acaagtttat gtatttgacc attacaaaga
     3241 tgagttatat atcatcgcga caaatcaatt ttcaaattca acaaaatcag atcttgagaa
     3301 tcgagttaat aagtctatcg aagacttaac taaaatccaa ccattcatgc ctacacaaga
     3361 ttttgatttt aaaactaaag aaattcaatc aaacatttct gaagaaaggt ttatcgaaat
     3421 gatacagtat ttcaaagaga aaataacaga aggggatatg ttccaagttg tgccatcaag
     3481 aatttacaaa tatgcgcatc atgctaatca gcatttaaat caactttcgt ttcaactgta
     3541 tcaaaattta aaacgacaaa acccaagtcc atatatgtat tatcttaata tcgatcaacc
     3601 atatattgtc ggtagttctc ccgaaagttt cgtaagtgtc aaagatcaaa ttgtaacaac
     3661 taatcctatt gcaggtacga ttcaacgtgg tgagacgaca caaatagata atgagaatat
     3721 gaaacaacta cttaatgatc ctaaagaatg cagcgaacat cgtatgctag ttgatttagg
     3781 acgtaatgat attcatagag taagtaaaat cggtacctca aaaattacta aattaatggt
     3841 tattgaaaaa tatgaacatg ttatgcatat cgtaagtgaa atcacaggta aaataaatca
     3901 aaatttatca ccaatgacag ttattgcgaa tttattacca acaggtaccg tttcaggtgc
     3961 accaaaatta cgtgcaattg aaagaatata tgaacaatat ccacataaac gaggcgttta
     4021 tagtggtggt gttggataca taaattgtaa tcataactta gattttgcat tagcaattcg
     4081 aacgatgatg atagatgagc agtatatcaa cgtagaagct ggttgtggcg ttgtatatga
     4141 ttctattcct gaaaaagaac tgaatgaaac gaaattgaaa gctaaaagct tattggaggt
     4201 gagcccatga tcttagttgt agataattat gattccttta cgtataacct agtggatatt
     4261 gttgctcaac atactgacgt cattgttcaa taccctgatg ataataatgt gctgaatcaa
     4321 tcggtggacg ctgttattat atctcctggt ccaggacatc cattagacga tcaacagtta
     4381 atgaaaatca tatcaaccta ccaagataaa cccattttag gtatttgttt aggggctcaa
     4441 gcactgactt gttattacgg tggagaagtg attaaaggcg acaaggttat gcacggcaaa
     4501 gttgatacac ttaaggttat atcgcatcat caacatctgt tatatcaaga tattccagaa
     4561 caattttcaa ttatgagata tcattcatta ataagtaacc ctgacaattt tccagaagcg
     4621 ttgaaaatta ctggacgtac cgaagattgt atacaatcat tcgagcataa agaaagaccg
     4681 cattatggta ttcagtacca tcctgaatca tttgctacag actatggtgt caaaataatt
     4741 actaatttta ttaatctagt gaagaaagga tgaaaaccat gacattacta acaagaataa
     4801 aaactgaaac tatattactt gaaagcgaca ttaaagatct aatcgacata cttatttctc
     4861 caagtatcgg aaccgatata aaatatgaat tattgagttc gtattcggaa cgagaaattc
     4921 aacaacaaga attaacatat attgtacgta gcttaattaa tacaatgtat ccacatcagc
     4981 catgttatga aggtgctatg tgtgtgtgcg gcacaggtgg tgacaagtca aatagtttca
     5041 acatttcaac aactgtagct tttgtcgtag caagtgctgg tgttaaagtc attaaacatg
     5101 gtaataaaag cattacctca aattcaggta gtacggattt gttaaatcaa atgaacatac
     5161 aaacaacaac tgttgatgat acacctaacc aattaaatga aaaagacctt gtattcattg
     5221 gtgcaactga atcatatcca atcatgaagt atatgcaacc agttagaaaa atgattggaa
     5281 agcctacaat attaaacctt gtgggtccat taattaatcc atatcactta acgtatcaaa
     5341 tggtaggcgt ctttgatcct acaaagttaa agttagttgc taaaacgatt aaagatttag
     5401 gcagaaaacg tgcaatcgtt ttacatggtg caaatggtat ggatgaagca acactatctg
     5461 gtgataattt gatatatgaa ttgactgaag atggagaaat caaaaattac acattaaatg
     5521 cgactgatta tggtttgaaa catgcgccga atagcgattt taaaggtggt tcacctgaag
     5581 aaaacttagc aatcacactg aatattttaa atggtaaaga tcagtcaagt cgacgtgatg
     5641 tggttgtctt aaatgcaggt ttaagcctat atgttgcaga gaaagtggat accatcgcag
     5701 aaggcataga acttgcaact acattgattg ataatggtga agcattgaaa aaataccatc
     5761 aaatgagagg tgaataatat gacgatttta gcagaaattg ttaaatataa acagtcactt
     5821 ttacaaaatg gctattatca agacaaactt aacaccttaa aaagtgtgaa gattcagaat
     5881 aaaaaatctt ttataaacgc aattgagaaa gaaccaaagc tagcaattat tgcagaaatt
     5941 aaatcgaaga gtcctacagt taatgactta cctgaacgag atttatcgca acaaatctca
     6001 gattatgaaa agtacggtgc aaatgccgtg tccattttaa ctgatgaaaa gtactttgga
     6061 gggagctttg aaagattaca agcattgaca acaaaaacaa cattacccgt attatgcaaa
     6121 gattttatta tagatccgct tcaaattgat gttgctaaac aagctggtgc atctatgatt
     6181 ttattgatcg ttaacatctt atcggataaa caattgaaag atttatataa ctacgctata
     6241 tcgcaaaatt tagaagtgtt agttgaagta catgatcgtc atgaattaga acgtgcctat
     6301 aaggttaatg ctaaattgat tgatgtaaat aacagggact taaaacgatt tgtcacaaat
     6361 gtggaacata caaatactat tttagaaaat aaaaaaccaa atcattatta tatttctgaa
     6421 agtggtattc acaatgcatc cgatgtaaga aaaatcttgc atagtggtat cgatggctta
     6481 ctaataggtg aggcgcttat gcgttgtgac aatctatctg aatttttacc acaactaaaa
     6541 atgcaaaagg tgaagtcatg atgaaattga aattttgtgg ctttacatca atcgaggatg
     6601 ttacagcgac cagtcaatta cctattgatg cgataggttt catccattat gaaaaaagta
     6661 aaaggcatca aacaattacc caaataaaaa agttagcgtc tgctgttcca gaacatattg
     6721 acaaagtatg tgtcgtggta aatcctgatt taacaactat tgaacacgta ttaagcaata
     6781 cgccaattaa cacaatacag ttacacggca cagaatctat tgattttata caggaaatta
     6841 aaaagaagta ttcaagcatt aagattacta aagctttagc tgcagatgaa aacataatcc
     6901 aaaacataaa taaatataaa gggttcgtag atttatttat tatcgacaca ccctcagtgt
     6961 cgtatggtgg taccggtcaa acatatgatt ggactatttt gaagcacata aaagacatac
     7021 cttatttgat agcaggaggc attaactctg aaaatattca aacagttaat caacttaaat
     7081 tatcacatca aggttatgat cttgcatcag gtatagaagt aaatgggcga aaagatatag
     7141 aaaaaatgac agcaattgta aatattgtga aaggagatag agaaaatgaa taaacaaata
     7201 caaacagaag cagatgaatt aggtttcttt ggtgaatatg gagggcaata tgttccagaa
     7261 acattaatgc cagcaattat tgagttgaaa aaagcttata aagaagtaaa agcagaccca
     7321 gagtttcaaa gagaactgga atactattta tcagagtatg taggacgcgc gacgccactt
     7381 acatatgctg catcatatac tgaaagccta ggtggcgcta aaatatattt aaaacgagag
     7441 gatctaaatc atacaggcgc acataaaatt aataatgcgt taggtcaagc gttgctcgct
     7501 aaaaaaaatg ggcaagaaga agcttgttgc tgaaacaggt gcgggtcaac atggtgtagc
     7561 tagtgctacg gttgctgcat tatttgatat ggaacttgtt gtctttatgg gaagtgaaga
     7621 tattaaaaga caacaactta atgtatttag aatggaatta cttggtgcaa aggttgtggc
     7681 agttgaagat ggtcaaggga ctttatcgga tgcagttaat aaagcattgc aatattgggt
     7741 aagtcatgta gatgacacac attatttatt aggttctgca ttaggtccag acccattccc
     7801 aacgattgtt agagattttc agagtgtgat tggtaaagaa ataaaatcac agatattgaa
     7861 gaaagaaggt cgacttccgg atgcaattgt agcatgtatc ggtggcggct caaatgcgat
     7921 cggtacattt tatccattta ttaaagacga tgttgcgtta tacggtgttg aagccgcagg
     7981 tcaaggcgag gatactgata aacatgcact tgcaattggc aaaggatcac ctggcgtatt
     8041 acatggtact aaaatgtatt taattcaaga tgaaggtggg caagtgcaac tagcacattc
     8101 tatttcagca ggacttgatt atcctggtat tgggccagaa cattcttatt accacgacat
     8161 tggtagagta acttttgaaa atgcaagtga tacacaagca atgaatgctt taatcaactt
     8221 tacaaaacat gaaggtatta tacctgcaat tgaaagtgca catgcactga gttatgttga
     8281 aagactagcg cctacgatgt cgaaagaaga tattattgta gtaactattt ctggacgtgg
     8341 cgataaggat atggaaacaa ttagacaata tatggcagag cgaggtcttg caaatgacta
     8401 aattatttat accttatatt atgggcaata aagatttgat tgaaaatgca acattgttga
     8461 gtgaaaatgg tgcagatata attgaaattg gtgtaccttt ctctgatccg gttgctgatg
     8521 gtccagttat catggaagca ggtcaacaag cgattaaaca aggcataacg atagattata
     8581 ttttcgagca attagaaaaa cacggtaatc aaattaagtg tcagtatgta ttaatgacgt
     8641 attataatat tatttgtcat tatggagaac aagcgttttt tgaaaaatgt cgagataccg
     8701 gtgtctacgg cttaattatt cctgatttac catttgaatt atcgcagcgt ttaaaacaac
     8761 aatttagtca ctatggcgtc aaaattatat cgttagttgc gatgactact gatgacaaac
     8821 gtataaaaga aatcgtatcc catgcggaag ggtttattta tactgtgacg atgaatgcga
     8881 caacaggaca aaacggtgcg tttcgcccag aattaaaacg aaaaattgag tcaattaaag
     8941 cgatagccaa tgtgccagtt gtcgcaggat ttggtataag aactccacaa catgttgcag
     9001 atataaaaga ggtcgcagat ggtatcgtta ttggtagtga aatcgttaag cgatttaaag
     9061 ctaacacgcg tgaagaaatc attaaatatt tacaatctat ccagcatgca ttgaataatt
     9121 aagtttactt gatttaaaaa aattagtcga atactgtttg aaaaagtgaa aaacggtgaa
     9181 ttataaaatt gaatagaaat gatacaaaag taatatgagc aaacccaaac gttcatatta
     9241 ctttttttga aattgtatgc aaaaatctaa atattactat aaaagtatat gcaattaaag
     9301 cgtttatgtt ttagttttaa cattaactat tgtatactta tttagattag atttattatt
     9361 tttgataatt gcagagggga aatagaaaaa ctgcaaatac ggaaatgaaa ttaattaacg
     9421 agagacaaat aggagtaatg ataatgaagt ttacaaattt aacagctaaa gagtttggtg
     9481 cctttacaga tagcatgcca tacagtcatt tcacgcaaac tgttggccac tatgagttaa
     9541 agcttgctga aggttatgaa acacatttag tgggaataaa aaacaataat aacgaggtca
     9601 ttgcagcttg cttacttact gctgtacctg ttatgaaagt gttcaagtat ttttattcaa
     9661 atcgcggtcc agtgatcgat tatgaaaatc aagaactcgt acactttttc tttaatgaat
     9721 tatcaaaata tgttaaaaaa catcgttgtc tatacctaca tatcgatcca tatttaccat
     9781 atcaatactt aaatcatgat ggcgagatta caggtaatgc tggtaatgat tggttctttg
     9841 ataaaatgag taacttagga tttgaacata ctggattcca taaaggattt gatcctgtgc
     9901 tacaaattcg ttatcactca gtgttagatt taaaagataa aacagcagat gacatcatta
     9961 aaaatatgga tggacttaga aaaagaaaca cgaaaaaagt taaaaagaat ggtgttaaag
    10021 taagattttt atctgaagaa gaactaccaa tttttagatc atttatggaa gatacgtcag
    10081 aatcaaaagc ttttgctgat cgtgatgaca aattttacta caatcgctta aaatactaca
    10141 aaaatcgtgt gttagtgcct ttagcgtata tcaactttga tgaatatatt aaagaactaa
    10201 acgaagagcg tgatatttta aataaagatt taaataaagc gttaaaggat attgaaaaac
    10261 gtcctgaaaa taaaaaagca cacaacaagc gagataactt acaacagcaa cttgatgcga
    10321 atgcgcaaaa gattgaagaa ggcaaacgtc taaaagaaga gcatggtaat gaattaccta
    10381 tctctgctgg tttcttcttt atcaatccat ttgaagttgt ttattatgct ggtggtacat
    10441 caaatgcttt ccgacatttt gccggaagtt atgcagtgca atgggaaatg attaattatg
    10501 ctttaaatca tggtattgac cgttataatt tctatggtgt tagtggtaaa tttacagaag
    10561 atgctgaaga tgctggtgta gttaaattta aaaaaggtta taatgctgaa attattgaat
    10621 atgttggtga ctttattaaa ccaattaata aacctgtata tgcagcatat actgcactta
    10681 aaaaagttaa agatagaatt ttttaggaaa ggaattatta aaacatgaaa tttacagagt
    10741 taactgttac tgagtatgac aactttgtac aaaatccatc gttggaaagc cactatttcc
    10801 aagtaaaaga aaatattgtt acccgtgaaa atgatggctt tgaagtagtt ttattaggta
    10861 ttaaagacga caataacaaa gtaattgcag caagcctttt ctctaaaatt cctactatgg
    10921 gaagttatgt ttactattcg aatcgtggtc cagtaatgga tttttcagat ttaggattag
    10981 ttgattatta tttaaaagag ttagataaat atttacagca acatcaatgt ttatatgtta
    11041 aattagatcc gtattggtta tatcatctat atgataaaga tatcgtgcca tttgaaggtc
    11101 gcgagaaaaa tgatgcctta gttaacttgt ttaaatcgca tggttacgag catcatggct
    11161 ttacaaccga atatgatacg tcaagccaag taagatggat gggtgtgtta aatcttgaag
    11221 gtaaaacacc tgaaacattg aaaaagacat ttgatagtca acgtaaacgt aatattaaca
    11281 aagcgataaa ctatggtgtt aaagtcagat tccttgaacg tgatgagttc aatcttttcc
    11341 tagatttata tcgtgaaact gaagagcgtg ctggatttgt ttcaaaaacc gatgactatt
    11401 tttataactt tattgacaca tatggcgata aagtattagt accattagca tatattgacc
    11461 ttgatgaata tgtgttaaag ctacaacagg aattgaatga caaagaaaat cgtcgtgatc
    11521 aaatgatggc gaaagaaaac aaatcagata aacaaatgaa gaaaattgca gaattagata
    11581 agcaaattga tcatgatcag catgaattat tgaatgcgag tgaattgtgc aaaacggacg
    11641 gcccaattct aaaccttgct tctggcgttt attttgcaaa tgcatatgaa gtgaattatt
    11701 tctctggtgg ttcatcagaa aaatataatc aatttatggg accatacatg atgcattggt
    11761 ttatgattaa ttattgcttc gataatggtt atgatcgtta taatttctat ggtttatcag
    11821 gtgattttac agaaaacagt gaagattacg gcgtataccg ctttaaacgt ggatttaatg
    11881 tacaaatcga agaattaata ggggatttct ataaaccaat tcataaagtg aaatattggt
    11941 tgttcacaac attggataaa ttgcgtaaaa aattaaagaa atagatgtaa atgatgttag
    12001 ggcttatagt tattgatact ataggctctt ttttatatgt tttttagcga aattttaatt
    12061 atttcacact aagaaaaact agtatgtatt tgtcaatcta atatttttat ttataaaatg
    12121 tattagagag tatatagtgc catatttgag ttttaatgat aatattttta taatatataa
    12181 tataaaaata tggttatctg agtatgaaat gcaagtaagt gaggtagtta aaaaatgaat
    12241 c
//
LOCUS       DAHHRR010000078        12212 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.078, whole genome shotgun sequence.
ACCESSION   DAHHRR010000078 DAHHRR010000000
VERSION     DAHHRR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.078"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(192..1550)
                     /locus_tag="NBI42_001820"
     CDS             complement(192..1550)
                     /locus_tag="NBI42_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCD1778232.1"
                     /translation="MFNEIWPLISVVLGIAILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGEVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLIATITQLVTGHDKPTNLIESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(1667..3220)
                     /gene="gntK"
                     /locus_tag="NBI42_001821"
     CDS             complement(1667..3220)
                     /gene="gntK"
                     /locus_tag="NBI42_001821"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCD1778233.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYIMRESKVNQDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTTKFADIKT
                     YIFYHLFDRYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPEIVPTTYVMKGM
                     KKRYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIKENVAVYQDLVSIFINLSRSLTENYEQIADFQR
                     QHMAENKTQ"
     gene            complement(3244..3924)
                     /locus_tag="NBI42_001822"
     CDS             complement(3244..3924)
                     /locus_tag="NBI42_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1778234.1"
                     /translation="MMYGYPEKWLEGMTTGESIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNRERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(4078..4842)
                     /locus_tag="NBI42_001823"
     CDS             complement(4078..4842)
                     /locus_tag="NBI42_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1778235.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNETSTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIMSA
                     ILMKNKWPFLIALPFMIGYGIGITIYYQRKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISRIQHNN"
     gene            complement(5007..5699)
                     /locus_tag="NBI42_001824"
     CDS             complement(5007..5699)
                     /locus_tag="NBI42_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCD1778236.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(5930..6346)
                     /locus_tag="NBI42_001825"
     CDS             complement(5930..6346)
                     /locus_tag="NBI42_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCD1778237.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGVVRQREPLMDGPHPKVVESFKDV"
     gene            complement(6485..8308)
                     /locus_tag="NBI42_001826"
     CDS             complement(6485..8308)
                     /locus_tag="NBI42_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD1778238.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLKVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSDENVRYWRKEVTVSEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFNFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLATFENIAK
                     HLELNSELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKSV"
     gene            complement(9219..10496)
                     /locus_tag="NBI42_001827"
     CDS             complement(9219..10496)
                     /locus_tag="NBI42_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1778239.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            11043..11654
                     /locus_tag="NBI42_001828"
     CDS             11043..11654
                     /locus_tag="NBI42_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCD1778240.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            complement(11663..11761)
                     /locus_tag="NBI42_001829"
     CDS             complement(11663..11761)
                     /locus_tag="NBI42_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778241.1"
                     /translation="MPNKLKKAVNHFNRWNDSLLFVYRNLKFMSLC"
     gene            complement(11751..>12212)
                     /locus_tag="NBI42_001830"
     CDS             complement(11751..>12212)
                     /locus_tag="NBI42_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1778242.1"
                     /translation="YDIGVGISGVLFLPITVSVILGSLFYKRLSKKYNEIMILRVTIT
                     GFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGKVLGVFN
                     FVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIIIFLYIKIGDFQLKYAK"
BASE COUNT         3907 a         2315 c         1762 g         4228 t
ORIGIN      
        1 gtttcataat tatcgcctgt acaatacgca caaaaacatg tcttgaaacg cctttcatca
       61 ctctaaaata accaatatac tttttatatt gttcggtttt taactatttc agacgatttt
      121 ccgcataaaa ataaacgtgt ttcaaggcaa tatattgcaa ttacctaaaa acacgtttac
      181 ttaatattta gttaaacaaa taagctgatg aataaaatga agatgatacc tgaaacggaa
      241 ataattgttt ctaataatga ccatgttaag aatgtttctt ttacagttaa accgaaatat
      301 tctttaaaca tccaaaatcc tgcatcgttc acatgagaca aaatcacact acctgcacca
      361 atcgcaagta caactaatgc tacatttaca tctgaagatt gtaataatgg taagacaata
      421 cctgttgttg aaatcgcagc tactgtagcc gaacctaatg cgatacgtag tacagctgca
      481 acgatccacg ctagtaaaat cggagacatc tctgtacctt caaacatttt agcaatggta
      541 tttccgacac caccgtcgat taatacttgt ttaaaagtac cgccaccacc aataatcaat
      601 aacatcatgc cgattggata aatcgcattc gtcactgatt ccataatatg attcatctta
      661 cgttttctca ttaatcccat cgtaacgatt gcaaataata ctgctattag catggctgtc
      721 cctgctgttc caatcatata aatgatagat tcaattagat ttgtaggttt gtcgtgcccg
      781 gttacaagtt gcgtaattgt agctatcagc atcaatatta ctggtagtgt tgccgttaat
      841 aaactcatac caaatcctgg catctcttga tccgtaaatt ctttttgtgc acctaacgct
      901 gaaatatctc cttctcttgt atatgcagac ggaatcattt tttgtgcgac tttattgaaa
      961 atcggacctg caatgagtgt aactggaatg gcaataatca taccatataa taatacttca
     1021 ccaacatttg cctttaattc ttttgcgatg actaccggtc ctggatgtgg tggtaaaaag
     1081 ccatgtgtca ctgataaagc tgttaccata ggtagtccta gttttaacac tgaaacattt
     1141 gcgcgttttg ctactgtaaa tactaatgga atcagtaaga ctaaacctac ttcaaagaac
     1201 aatgcaatcc cgacgataaa tgctgcaaca agcattgccc attgtacatg tttttgacca
     1261 aatttttgaa tcaacgtgtc tgcaattcga gttgcaccac caccatcagc aagtaatttc
     1321 ccaagtatgg cacctaaacc gaatatcagt gcaatgtggc cgagcgtact gcccatccct
     1381 ttctcaatcg tctccataat tttagtcaat ggtataccta gcattaacgc tgtaatcatc
     1441 gatgtgataa ttaatgaaat aaatgtattt aatttaaacc caataattaa tactaataaa
     1501 atagcgatac ctaaaacaac actgattaac ggccatattt cgttaaacat gacattcccc
     1561 tctttctctt ttcaatagaa tgtaacaccg tcgtcgcgca cgtattccat ataatatata
     1621 gtagtcatgg cacaacaact ttatctaaga tgcttgagtg acgtatttat tgtgttttat
     1681 tttcagccat atgttggcgt tgaaaatctg caatttgttc ataattctct gttaaagaac
     1741 gacttaaatt gataaaaatg gatacgagat cttgataaac agcgacattt tctttaatcg
     1801 gcgtatgatt gtttgtggca ccgaccatcg atgaaacgat tgaaaaatct tcaatgtcac
     1861 ctacagcttt aagcccgagt acgcaggcac ctaagcatga actttcataa ctttcaggaa
     1921 ccactaactc tgtgtcaaat atatctgaca tcatttgacg ccatacttca cttttcgcaa
     1981 aaccacctgt tgcttttatc atcttaggtg tttcattcat tacttcaata agcgcaagat
     2041 agacggtata caaattgtaa agaacacctt ctaatgcagc gcgaatcata tgttcttttt
     2101 tatgagataa agttagaccg aagaatgaac ctcttgcatt tgcgttccaa agcggtgcac
     2161 gttctccagc taaataggga tggaatatta agccatcagc gccaggttta acacgcttag
     2221 caatttgagt taaaacatca taaggatcaa caccgagacg tttcgcagtt tcgacttcgc
     2281 ttgctaacaa ctcatcgcgc aaccatctca atacgacacc accattattt actggcccac
     2341 caatgacata gtggtcttct gttaagacat aacaaaatat tcttccttta taatctgtac
     2401 gtggtttatc tatcacagtt cgaatcgcac cagatgtacc gatagtgaca gcaacttctc
     2461 ctttgccaac actattgaca cccaaattag aaagcacacc atcgctagca ccaatgacaa
     2521 aaggtgtatc ttcattaagc cccattaatg ttgcataacg ttttttcatg cctttcatca
     2581 cataagttgt tggtactatt tctggtaaca tttccttgga aatacccagc agttctaatg
     2641 cctcaacatc ccaatctaat gtttctaaat taaacatccc tgttgcggaa gccattgaat
     2701 aatcaatgat atatctatca aacaaatgat agaaaatgta tgttttaata tctgcgaatt
     2761 tagtagtacg ttgaaataca tcttgccatt catgtttcat ccaaaaaatc ttcgctaatg
     2821 gcgacatagg atgaataggc gtacctgttc gctgataaat cgcattgcca tcatgcactt
     2881 catttattac tgttgcatat tttgcagcgc ggttatctgc ccaagtaata ttatttgtta
     2941 atctttgatg ttgctgatcc atcgcaatca agctatgcat ttgcgcacta aatgacacaa
     3001 atttaatatc atcttgatta actttcgatt ccctcattat gtatttaatt gtcattaata
     3061 ccgcatcaaa taattcatct gggttttctt ctgagacatc aacgtttggt gtatgtaaat
     3121 catagccgat ttgatgtttc atgatgaaag ttccattttc atcatataag actgacttgg
     3181 tactcgtcgt tccaatgtcg acaccaatca tatatttcat gacaaatcct tctttctttc
     3241 attttaattc aaccaaaatc cttcaatatc tttaccaaca tcgtcaaaat ttaaatgaaa
     3301 cgcttctttc aaaatttgac tgtcgtattg ttccactgca tcaataaaca cttgatgatt
     3361 atgatgtatg cgttcaaaat cttgcgggtt ttgttgcata cgttgtcgca tggatgttaa
     3421 aacgagcgcc atcattactg gttttaaatg actccaaaat gagtttaaat attgatgatt
     3481 agatgctttg attaatgttt catgaaattc aaagtcatgc ttcgtaaatg attctgcatc
     3541 ttcaaacttt actgccactt tcatcatttc aagttgtttc ttcatttctt ttacgatagg
     3601 tagtcgctct cgatttttaa ctcttgaaaa tgcaaatgac tctagcatca gtcgcaaatc
     3661 gtacatttct ttcttttctt gttccccaaa cggcaacaca tgtgcaccca ttctttctaa
     3721 ttggatgagt tgattttgtt gcaataattt aaatgcatct cgaattggcg aacgactcac
     3781 attaaattgc tttgccattt gattttcagt gagtaacgta ccttcagcta tgtgaccatt
     3841 cacaatgcct aagcgtaatt ctgctgcgat actttctcca gttgtcatac cttccaacca
     3901 tttctctgga tatccataca tcatcaaagc cactccttca ttacacgaca tacttgtata
     3961 caagtatgtt aatatagttc ttaatcgttt gcaagcgctt tctttacgcg cactaaaata
     4021 gtgaccaccc cttttcgatg taagtttaaa ggggatggtc actatcacac gaatgattta
     4081 attgttatgt tgtatgcggg atatttctaa ttgttctgta ctcatatgcg ctttaggtac
     4141 ttcaatgcaa taatgtgttt catgacagtt tggacattcg aatcgacgtg ttgtcgctgt
     4201 atgtttcgct ttgataactg cccacaaaga cggtgagaat atatgctggc agttagggca
     4261 taaataggca actttgcgtt gataatagat agtaatacca atgccataac cgatcataaa
     4321 tggtaaagca attaagaacg gccatttatt tttcatcaaa attgcactca taatgctaga
     4381 atattgaatt attcctataa taccagcact aatccaaatg ttacgacgaa tacttttcat
     4441 ttcagctgat ttactcatga catgctctat gtcttttaag tgtgtgattg gagacgtcga
     4501 cgtttcattt acgtaatatt gaacattttt aattttgttt aataccgctt gttgctgttt
     4561 gacttgttgg ttaatttctt gttgtttcat agttagtaaa gtattgagcg tcttcaaagt
     4621 accttcacct tttagcaaca tatctatatc gcttaacgca caacctaaat ctttaagcaa
     4681 taagattaac tctaatgttt gtcgctgttg ttctgtatac acacgacgct ttccttctgt
     4741 aaatccttgt ggtttcaaaa tacctttgcg atcataatat tgaatcgttc gtgttgtcac
     4801 attgcataat tttgcgagtt ctccagtcga atagttagac atagactcca cctcctacaa
     4861 tcaccatagt tgatgacccg acgtcacgag caagtacaat ttccacattt taaaggaatt
     4921 tattatacta ggcgtcttat ttttgtgatt tcgtaccatg ttgatttaca aaatcactca
     4981 aattaagcaa cactcctact aaacgtctac tctgttattt cagaatgaat ttgttgtaat
     5041 ttatcttcaa cttcagtaat ctctgtcgca cattctttca ataaatctcg atacttttcc
     5101 gtctctgcat tgtttttata acgtatttta tgttctaaac ttgcccacat atccatacct
     5161 atcgttctaa tttgaatttc aacaggcaat acctcaacac gttctgctaa aaagactgga
     5221 atggatacaa cgatatgtaa actgcggtaa ccattttctt tagggtgctg aatataatct
     5281 ttacgtttta tcaattgtac gtcttcttgt ttaagcaaca tctcttcaat caaataaata
     5341 tcatctaaat aattacatac tacgcgaatt ccggcaatat ccagtatgtg ctctttggca
     5401 gtttctgcgc taatctgtaa tcctttacga ttaagctttt ctattaaact acgcatttct
     5461 ttcacacgtc gctccatatg atgtataggg ttgtgtttat aaatgtgatt gaaattgtcg
     5521 tctaaaatac tcagtttagt gcttatttcc tttagtgctg atgaataaat atgatctaac
     5581 tctacgaaac ctaataacgt atcaaatgct tcatcaccat tttcaaattt acttaaacta
     5641 tttttaaaat catgtcgcaa atcctctaaa tataatgatg gttttcgatc tacatacatt
     5701 tttatactta cctccgatat ataatcatct ttattgtacc taacatttta ttaagataat
     5761 actaatacac tatcgaactt tgggctgata ctgaaacagc acaaagaaac tcaaacaatt
     5821 cgaattatgt atcaaaacct tcaattatag gaagtaattt taaattgatt taacgcaaaa
     5881 aaacacctgt taccgttata taggtacaag tgcttaattg atagagatgt tatacgtctt
     5941 taaatgattc cacaactttt ggatgtggac catccataag cggttctctt tgtcgaacga
     6001 caccactttg gttgccaatt gattcgctat caaagtacga tttatcttga tttgattgtt
     6061 cttgaatatg ttcttcatta tcagttgttg catattcact atccacctct gttttttcca
     6121 ttgttgctgt atggaattgt acgaagttct cttcttctaa tgctttgatt tcttctttcg
     6181 ataacgctcg ataccaatct ttcgcttttt tcgccgcaat aggaacaaca tctttttcaa
     6241 gatatccttc ttttaacatt gcattcgcaa tttctatcgc tttttgccgt ttaattactt
     6301 caaattcttt ccatttttcg gggtaacctt tcattgtaaa gggcatttca ttaacctcca
     6361 atatgttata attcatgtta tataccacca taattataaa atgaaacata ttaaatacaa
     6421 ttttttagac tcccgtcata ataacttcag acaaacgtta gtctgacctg ataaatattt
     6481 tattttaaac gctcttgtct ctctcacgaa aaccattgtg ttgaatattc atcattgctg
     6541 attgaatata attatactgt ggtaaatctg gtaatatctt tatttctaat tctgaattta
     6601 attctaaatg cttagcgatg ttttcaaatg ttgctaaata ctcgcccatt aattgttcat
     6661 ttattgtaag tctatcttca ttagccatgg ctctatttaa cataaagcta atttcttcta
     6721 atgcgaataa gctaggataa taattttgaa tcaatgtctt atcactaaat aattcaccat
     6781 tagctgcatt ataaacttgc gtcatgttat ttaatttcac agataggttc atagccgtgt
     6841 ttttctgata cacatcatct ttatattggt tttcagaaaa taaataatgg aagagtgttg
     6901 cttctttacg aacaacttca gccattacat taggaagcaa tgcggatgcg gtacgttgtc
     6961 caaggacaaa taaacctatg attgcaatca caatacccat tgcaacgtca ataactcttg
     7021 gaaacgcaat gttaattgtt aaattctgtg atgctaagcc gttcattaaa ataacttgta
     7081 ttgtaataaa aactactgca atcgcataat ttgctcccac caatgcttca gtaaacaatg
     7141 ctgcaatgcc cattaaaatt actgcaacag gtgttggtat tgaaaataac aatataacag
     7201 aaagtactaa cacacctaaa atagtaccta aacctcgtgc catacctctc tcgattgcat
     7261 gtatagttga tgttcctagt aatattgtat gtgcagataa cggtatccag tatgcttttt
     7321 caaaattaaa cattaacgca ataaatatcg ctatcatcat aatcgctgta tatctcaatg
     7381 tatttctaaa aacaatagag tctaatgtta aattttgata aatgcgttta ctatataaag
     7441 gttttcgcat gtctgcttca tacgcaatac gcgcttcatt tgcatgcacc atttcatcaa
     7501 ttttcaatat atggttgaat aaattttgaa attcttctga tactgtcact tcttttcgcc
     7561 aatatcttac attttcatca ctattatcta gttgtgcaat gatatgatcc atcatttcaa
     7621 ttaactcatc tggcaatggt ctaatttgtt tagcgttcaa ctctaacagt tcagaataaa
     7681 tcccttgggc agatgtgtgt aataataata atttttggaa acgtctactt aatttatcgt
     7741 tacttgaagt agaagtgatt aataatttat cagatgcttt aaaactatcc actgctgttt
     7801 ttgctacttt taaaaaagca gatttatcat tataagtgtg taacaactta gatatgagtt
     7861 taaaatcatt ttgaattgct tgttcttcag ctttgttttt agaaaatacg attgtgatta
     7921 acaccatcat agtggcaagt ataccgccta ctaaaatcgc aaagcctcta tataatgctt
     7981 cttcgggagc tataggtaag ttaatgggta gactgaatgt cacaatgaag aatgtcgatg
     8041 acggtccagc tatttttaag gcagtaaata tataaaatgg tataaccgta acaattaata
     8101 atgtcattcc aaaaacgagt ggcgttttgg ctgttagcgt accaagcatc atacatattg
     8161 caaacgctaa attacaaatt attacagttc gcagcttaga tcgcgacgga cctttaaaaa
     8221 cataaatatg ggctagtgtt ccagttgcaa ctaataatcc aaattggaaa ttaccacata
     8281 agtaaccgat tattgcagga ataatcatta acagtccctg tcttacacct ttataaaagt
     8341 ctaagttaag tcgattgatt tttatgagtg atgtcaaaaa gatgtcattt cgacttctcc
     8401 tttccttgtc aaaatatttc cagaatcttt ttattagcat agttaaaatg aatttgttgc
     8461 tataaattta aattacgcaa catatgtcat tataacacct tatgctcaat tatctaaatt
     8521 taaaaacttt taaagtccaa ttaaaattat tttctaatgt tatttaacat cgttgaacct
     8581 ttggtcgccc acctttgcgc tagttaaaat ttatttgtga catgttgaga ttattttagt
     8641 gaattataca tttagggtta gaaatacatt cacatagact tagtgtctat ttacttcata
     8701 ttattcctag ggttcaagat ccaaactata gtgaatttaa gaaagtttat aaaacatatt
     8761 tgcctcataa gcttgaatag ttcctatatt cgttatttaa aatgacattc gatgtcagtt
     8821 catttcttaa aatatcgaaa gatgcttacg tataatatat atagtgacgt atttacaaat
     8881 aatggcagta agcagtacaa aatgattaca caaaaatttc cttctactcg ataaattaag
     8941 aagtataaaa taattaaaaa aatacttata attagaccta taagaggcaa acttctaata
     9001 tatttctcta agttttcctt catatgtata acctcctaaa atttatatgt acttttacaa
     9061 tagttacctg actaatgttt taaaacttta tttcttctac atatatcatt ttattaactt
     9121 cccgttcatc ttaaaccata tctgttttaa aatttttcaa ttttaacgta aaaaaagcaa
     9181 taagccccaa caatgtgaaa gcttactgct tgtttaaatt atttttctat tgctttatca
     9241 tcactaacaa ttaaaggttg atctggtttc acatagaacc acataatcac agcgattact
     9301 gctggaacga ttaacaattg gaatgtcatt gtccaaccta aatgttctac gaatagacct
     9361 gctaataacg gactgattaa tgcacctata ttaccccata agttcatcca tcctgatact
     9421 gtaccagaga agttacgtcc taagtcagtt gctgccgccc aactcatacc cattgaaata
     9481 ccgataccac caagacctag tgataaccag aaaatactta catataaatt tcctgtacgt
     9541 acagcaaaga taattgaaac tgcaaataca ataaatcctg cgattgcaat gacacctcta
     9601 gctacgaatt ttgaacgacc taatcctaat acacggtcag aaattgcacc tgctgataag
     9661 attaagaaga acattaataa ccaaggtaat gaactaatgc tcatttcttt aaagttaacg
     9721 tggaatactt ctgttaaata tgtcggtaac caaattaaga ataatgtaat gataaattgt
     9781 acaacaaagt attgaattgc aattgcatag aagctaaaac gcttaaagaa atcattccat
     9841 ggtggtgatg acttttcagt agctacgata tcacgatttt ccataatgaa acgtttctcc
     9901 gcttcattaa ccattctatg ttgctcaggt aagtctttgg caataatcgc ccataataca
     9961 gccattaaaa tacctactgc accaaaaatg taaaatactg cttgccagtt aaatgcgtta
    10021 acaatagcaa ttgtaactat tggtgctaat acaggtccga aatatgatcc tgctaataat
    10081 gcacttgatg ctctaccttt ttcattttta gagaaccaaa atgagttaaa cacagcatta
    10141 gaagggtaca ttggcgcctc acccacacca aataagaatc tcactaaata aattaaaccg
    10201 tggttcttaa tcatacccgt taagattgta aatgcactcc accaaactaa tgcaattgta
    10261 atcatcttac gtggaccgaa tttttcagcc aacatccccg atggtacttg cattaatgca
    10321 taacctagag agaagaatga tgccaataaa ccaaattgcg gttttgtcat ccctaaatca
    10381 tccatcattt gcttagcgat atatgaaatg tttgctctat ccatatacgc aataacccca
    10441 ataaagaaga acgcaagcgc gaacatccac cttacgttag ttcgtttttc cttcataact
    10501 attcctactt ccctaattaa ttttttaaaa cttaccctga aacgttaaaa ttttcggaca
    10561 tctggcatgt ccacccctac tttgtcacaa aattgtacca aagttatttt ttataatatt
    10621 atcagacatg gaaagcgttt acaatagaat ctataaaaat tttattaaat tccgcttaac
    10681 ttttgctatt aaaacgatat aaaggaactt ttagttacat agtagcccat catttgttaa
    10741 ttaaaaatac tgcaatacta gtgttttaaa acgtatattg caggctttct tacatatttg
    10801 acattcattt taaattgcat aaagctacat tgcttattca ttgtgcaacg cctgttattt
    10861 agatgattac ttgttgactt ttcgtactta tagcaagcaa ttttttgatt tattgcttat
    10921 aatacatttt aagacaaatg cttgtttcaa catcgcatac gacatcattc attattcatt
    10981 tgaatttatc cagtttctat ttgtctgcaa ttgctgaaaa taaaaggagc ttatttttaa
    11041 taatggaaca aattatcact gaatttatta gtcgttttgg gtatgcagcc atttttatat
    11101 taattttatt agaaaatgtt ttacctatcg taccatcgga aattattctg acatttgctg
    11161 gtcttatgtc tgttaaatca catttatcaa ttttaacctt atttattatt gcaaccatcg
    11221 catcgtttat aggactgtta attttatatt atatctgtcg tttgatttca gaagaacgtc
    11281 tatatcgctt tattgatcga cacggtaagt ggattaaatt gaaaagtaag gatttaaagc
    11341 gagcaaatga ttggtttaaa aagtatggcg tatgggctgt atttatctgt cgtttcatac
    11401 ctgtattacg tgtattgatt accattccag ccggtgtaaa ccgcatgaat gttgtgacat
    11461 ttaccgttat ttcattaata ggtacaacaa tttggaattt cggtttaatt ttactaggac
    11521 gcacgttgag cgatagcttt ggtatgttga tgactggtct tcatacatat tcacgtatta
    11581 tgtatgttgt catcattatc gcagtcattt actttgctat acgttacatc ggcaaacgta
    11641 agagagtaaa ataatattgt tgttagcata aagacatgaa ttttaaattt ctataaacaa
    11701 aaagcagtga atcattccat ctattgaaat gattcactgc ttttttaagt ttatttggca
    11761 tactttagct gaaagtcacc tatctttata taaagaaata ttattattaa catgatagtt
    11821 atagttgtga agtaaaaggc taccaacggc tgagaaatga taccaattaa taatgcaccg
    11881 acagtcattc caatatatct cacaaaatta aatacaccta gcacttttcc ttttatatgt
    11941 tcaaatcttt tagaaattat agtagacagc aaagctggca caataccaac acaagtacct
    12001 aatataaata taattactga taatataatt acatttgatt cgtttaacca tccaaacaat
    12061 aacgtaaata tagcaaatcc agttatagta acacgaagta tcattatttc attatatttt
    12121 tttgacaatc gcttataaaa taaacttcct aatataacac taacagtaat aggtaaaaac
    12181 aaaactcctg atattccaac gcctatatca ta
//
LOCUS       DAHHRR010000079        12084 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.079, whole genome shotgun sequence.
ACCESSION   DAHHRR010000079 DAHHRR010000000
VERSION     DAHHRR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.079"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            107..1114
                     /gene="aaa"
                     /locus_tag="NBI42_001831"
     CDS             107..1114
                     /gene="aaa"
                     /locus_tag="NBI42_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HCD1778243.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNATSSNSGSATTTNR
                     GYNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRP
                     TVGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYI
                     H"
     gene            complement(1299..1568)
                     /locus_tag="NBI42_001832"
     CDS             complement(1299..1568)
                     /locus_tag="NBI42_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778244.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            1718..2113
                     /locus_tag="NBI42_001833"
     CDS             1718..2113
                     /locus_tag="NBI42_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCD1778245.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYIGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            2103..2588
                     /locus_tag="NBI42_001834"
     CDS             2103..2588
                     /locus_tag="NBI42_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1778246.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(2758..3540)
                     /locus_tag="NBI42_001835"
     CDS             complement(2758..3540)
                     /locus_tag="NBI42_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCD1778247.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(3537..4655)
                     /locus_tag="NBI42_001836"
     CDS             complement(3537..4655)
                     /locus_tag="NBI42_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCD1778248.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(4770..5654)
                     /locus_tag="NBI42_001837"
     CDS             complement(4770..5654)
                     /locus_tag="NBI42_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1778249.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNIMLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPNLLDHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAESVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            5835..10334
                     /gene="gltB"
                     /locus_tag="NBI42_001838"
     CDS             5835..10334
                     /gene="gltB"
                     /locus_tag="NBI42_001838"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCD1778250.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEAVFKKYFEGEELSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSFL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVDAVKQGAQILVLDDSGLVDGNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRLEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEITKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATGGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHQSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            10352..11815
                     /locus_tag="NBI42_001839"
     CDS             10352..11815
                     /locus_tag="NBI42_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HCD1778251.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLTAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIA
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGQIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
BASE COUNT         4088 a         1822 c         2470 g         3704 t
ORIGIN      
        1 catcaaacat caacctatta tacataataa aatcgtataa tgatgtagta ttcataaatt
       61 cggataaaag aatgttagga aagttaagca agaggaggat tttaaagtgc aaaaaaaagt
      121 aattgcagct attattggga caagcgcgat tagcgctgtt gcggcaactc aagcaaatgc
      181 ggctacaact cacacagtaa aaccgggtga atcagtgtgg gcaatttcaa ataagtatgg
      241 gatttcgatt gctaaattaa agtcattaaa caatttaaca tctaatctta ttttcccaaa
      301 ccaagtacta aaagtatctg gctcaagtaa ttctacgagt aatagtagcc gtccatcaac
      361 gaactcaggt ggcggatcat actacacagt acaagcaggt gactcattat cattaatcgc
      421 atcaaaatat ggtacaactt accaaaacat tatgcgactt aatggtttaa ataatttctt
      481 tatttatcca ggtcaaaaat taaaagtatc aggtactgct agctcaagta acgctgcgag
      541 caatagtagc cgtccatcaa cgaactcagg cggcggatcg tattacacag tacaagcagg
      601 tgactcattg tcattaatcg catcaaaata tggtacaact tatcaaaaaa ttatgagctt
      661 aaatggctta aataatttct ttatatatcc gggtcaaaaa ttaaaagtaa ctggtaatgc
      721 aacatcttca aactcaggat ctgcaacaac tacgaataga ggttacaata caccagtatt
      781 cagtcaccaa aacttatata catggggtca atgtacatat catgtattta atcgtcgtgc
      841 tgaaattggt aaaggtatta gtacttattg gtggaatgct aataactggg ataacgcagc
      901 ggcagcagat ggttacacta tcgacaatag acctactgta ggttctatcg ctcaaacaga
      961 tgtaggttac tatggtcatg ttatgtttgt agaacgtgta aataacgatg gtagtatttt
     1021 agtttcagaa atgaactatt cagctgcacc aggtatttta acttacagaa cggtaccagc
     1081 ttaccaagta aataattata gatatattca ctaaagtctt acgtatataa atatataatg
     1141 aattcctatt acatttcaca agctgaaaag ttggattgaa tcctaaactt tatggcgggg
     1201 ctatcaaagt cgtgattgtt gtaataggaa tttttgtata agaaaaagga ttggtcgaat
     1261 gaacttcatg ttctatgttc gaccaatcct tttctgattt agtattcatg cttaactttg
     1321 cgaatgcttg taaataatct agcaccgttt gttattaaag taacaactgc cactgctttt
     1381 ttgaacttgc gtggtgactt aattgaaatg taaaagtcta acacggttcc tacagtgctt
     1441 aaacctaatg aaagatatac taatttttta tttttagcat gatatttata gccattgtag
     1501 ccgtcgacta tgaaacctgc gacatttagt aaacttgata aacgttgtga tttggaacgt
     1561 tttgccataa taataattcc ccctagtatc ttctgtttat atggaaaaat tgggtgcgct
     1621 gaattgctaa cgttttgcgc tataactact catatatgat aacataattg tacagtataa
     1681 tttgaaaaat tgatttcaca aagttggggt gtcaaagatg attaaatgtg tctgtttagt
     1741 tgaagaaaca gctgataaaa tattgcttgt tcaagtaagg aatcgcgaaa agtattattt
     1801 cccaggtggt aaaatagaag aaggggaatc acaagtacac gcactgttaa gagaagtaaa
     1861 agaagaatta aatttaacat taacaatgga tgaaattgaa tatatcggaa caattgtagg
     1921 tcctgcatat ccacaacagg atatgttaac tgagttaaat ggatttcgcg cattaaccaa
     1981 aatcgattgg gaaaacgtaa ctatcaataa tgaaattacg gatatacgct ggattgataa
     2041 agataatgat gcgttgattg cgcctgctgt caaagtttgg attgaaacgt atggtggtaa
     2101 acatgacaaa taatgacacc atcatgttac gacattatgt cccacaagat tattcgatgt
     2161 tagaagcttt tcaattaagt gaaagtgatt tgaagtttgt taaaacgcca gaggaaaata
     2221 ttacagctgc aatgtctgat aatgaaaggt atcccatcgt tgtaatggat ggcaggcaat
     2281 gtgtggcctt ttttacatta catcgtggaa aaggggtcgc accatttagc gataaccaag
     2341 atgcagtatt ttttaggtca tttagtgttg atcaacgtta tcgtaataga ggaataggta
     2401 aagtggtaat ggaaaaattg gcgtcattta tcacttcaac atttcaggat attaatgaga
     2461 ttgtgttaac ggttaatact gacaatccac atgccatggc actttatcgc caacaaggat
     2521 atcaatatat gggagatagt atgttcgtcg gaagacctgt tcatattatg gcgctaacta
     2581 taaaataaat taaatttaat agcatcttta ctcatcgtcg accacaacaa ttaatgatga
     2641 ataaaggtgc tttttgttat agaccaccgg acaatttact atagtaaaaa gcgacctagt
     2701 gaacaattga catatatcca caggtcgctt aactataagt tatattgcta gttgcgatta
     2761 attgatagac tcatcatttt tgcgctgtcg agatggtctt tttattaaaa atgccgtaat
     2821 ccaagccgta atcggaatac tgattgcaac ggcaataccg cctaaaataa tagaaataaa
     2881 ttcttgggca aatattttcg agtttataat atgaccaaat gaatatttaa gtttgaaaaa
     2941 ccaaataaat aaagcaagtt ggccaccaaa aaaggcaagg taaatcgtgt tcgcagatgt
     3001 cgctaaaatt tctctaccaa cacgcatgcc agattggaat aattcgtatt gcgtaagtgt
     3061 tggattcact tgatgcaatt cataaatggg tgaactaatg gtaatcgtta aatctatcac
     3121 agctgcaata acagcaagaa taatagtgaa caccataaat tgaaccatat caatgccaat
     3181 attcattgaa tacacatatg tttcatcttg ttgttcggtt gaaaagcctt gtagatgacc
     3241 gaagtagacc gataaataaa tgagtgtaat caacaatatt gttgtaacaa tagtgctgat
     3301 aaatgcagct tgtgttttaa cattgtaact attgagtacg aataaattac aagcgccaat
     3361 aataatgcag aaaaagaatg tgacgacata aatcggtacg ccaaaaataa tcaatacaat
     3421 gctaataatt aaaatagcaa aatttaaaaa tagggttaaa taagagatga atcccttttt
     3481 acctccgaaa attatcatca gaaagaggag caataacgcc aatataaata cagcattcat
     3541 tgtttcgccc tccttaatgt ttcaaatatt tccataaaca atattgtgat aggaattgta
     3601 agtacgatac ctataccacc tgttagtgcg cgcgcaattt ctaacgacca attcatcgaa
     3661 atagtataag tcactgtatt ggcatttttt aaaaagatta aaaacatagg tagtgcaccg
     3721 gataaatatg agaataataa gatgttagtc attgttccca taatatcttg gcctatgttt
     3781 cgcccagcaa gcgcccatct cctcattgaa atgtgcggcg tacgctgtaa aatttcatgc
     3841 attccactag caattgtaat tgcaacatcc ataatagcgc caagtgaacc tattaacact
     3901 gaggctagga agatatcttt cggtggtaat gataaaaagt tcatcgtttc atatttaatg
     3961 cctttaccat ctgtcatata tatgattaat tctgttaaac ctatactcaa aaaagttccg
     4021 ataattgtac tggctatggt aatgagtgta cgcatatgcc agcctgtaac gagcaataaa
     4081 gtgagtattg ttgaacagat catggcaatg gtcatgagta agaataaatt aatattgcta
     4141 tgttgaatat gaatgtaaat tgcgattaat atggcaatag aattcaagat taacgataaa
     4201 atcgattgca gtccgacttt gcgaccaacc aataatacag ttaataagaa caaaccagtg
     4261 atgataaccg ttaaggtatc acgcttcttt tctataatat aagcatcact cggcttgtta
     4321 gaaatatgta ataatacttt ttcgtgtgtg cgaaatgcct cagaatctgc ttgcgatttg
     4381 acgtactgat gattaatcgt cgtcgtttct ccagcaaatt gaccatttaa tattttgact
     4441 tttaattgat ttttatattt aatatcacga ttattttgtg catcttttgt aggtgtcgaa
     4501 gaaacatgtt tgacatctat aatttgacca attggtttgt tgtaaaagtt ctcattattg
     4561 aatgtaaata aaatagcacc aatgaatgcg atgcagaaca aacctaaaat tatattaaat
     4621 ggctttgtaa ataaatttct atatttcaaa aacaaaaccc caattctatg aatgaattaa
     4681 tatggtgatt atacgccctt aattttttat tttcaaagat attactgcta agtgtaaaac
     4741 gaaaatcatc attgatagcg tcgaattact taatggaatg tagacgtttt agtcattaat
     4801 tgttgaatga gtgttaataa gataccaata tcactcttcg tataaggctc ctttgtaata
     4861 gcacatatcg ttctttttaa ttcagtatga tctaatttta tatctatcca tgatttagat
     4921 tctggtaaat gtatattttg tgatgaaatg atgtaacctt ctttttgacg aaggagatac
     4981 tgcgcaagtg gttgactact gattgtgtat acatctgatt tagtaatctt acgcaattgt
     5041 ttttttacag attcggcaaa tggtgccaag caataaatat gactatgctc aaactgaatt
     5101 aatggtgggt gtgtcgccat cgtgattgga tcgtctgaag gcgcatataa atgatagtgc
     5161 tcttcgaata aaggtagcat atgtaattgt ttgtgtttac gtatttctgg tgtaagttcc
     5221 gtgaaaccaa tgtctatatt cccatttaat acactattta taatcgtgtc atgatctaat
     5281 aagttcggta tgacatgtgt atcattttgt aaatgaaacg tttggataag tggtagtaac
     5341 atgtgggata cgtcactctc atcatagcca atgtagatac ttttattttt agttaatcca
     5401 tggctttgaa attgttcaat cgtgctatct aaatgttcaa taatacgcag agcttcatta
     5461 aataataatt tcccctcaga tgtgagcata atattgcgtc cttgcttttt aaataaagac
     5521 acattaagtt cttgttctaa taatgtaatt tgacggctta tcgctgattg agcaatgttt
     5581 agttcaagtg ctgtttcgga gatatgttct cttttagcga cctcgataaa atatcttaat
     5641 tgtttaattt ccatagcgat ataggcacct ccgaaaatga gtgttttgta actattatag
     5701 caatattatt gatgaatgtt ctatttttta gatgaatatc ttctatttta tatattgaac
     5761 agataaattt tttagattat agtaattatc attaataact aatatcagaa tattctaaaa
     5821 aaggggtgtg catcatgcac aatgagaaat taattaaagg cttatatgac tatcgtgagg
     5881 aacatgatgc gtgtggtatt ggtttttatg cgaatatgga caataaaagg tctcacgaca
     5941 tcattgataa atcgcttgaa atgttgcgac gcttagatca caggggcggg gtcggcgcag
     6001 atggcatcac tggtgatggc gcaggtatta tgactgaaat accttttgca tttttcaaac
     6061 aacatgtaac ggactttgat atcccaggtg aaggtgaata tgccgtgggg ttattttttt
     6121 ccaaagaacg cattttaggt tctgaacatg aagcagtttt taaaaaatat tttgaaggcg
     6181 aagagttatc aattcttggt tatcgtaatg taccagttaa taaagatgcc attgctaaac
     6241 atgtagcaga tacgatgcca gtcattcaac aagtgtttat tgatattagg gacattgaag
     6301 atgttgaaaa gcgtttgttt ttagcgagaa aacaattaga gttctattcg actcagtgcg
     6361 atttagaatt atattttacg agcttatcac gcaaaacaat tgtatataaa ggttggttac
     6421 gatcagacca aattaaaaaa ctctatacag atttatcgga tgatttatat caatcaaagc
     6481 tagggttagt gcattcgaga tttagtacga atacattccc gagttggaaa agggcgcatc
     6541 ctaaccgtat gttaatgcat aatggtgaga ttaacacgat taaaggtaat gtgaactgga
     6601 tgcgagcacg ccaacataaa ttaatcgaaa cattatttgg tgaggatcaa cataaagtgt
     6661 ttcaaattgt cgatgaggat ggtagtgact ctgctattgt agataatgcg ctagagttct
     6721 tatcgttagc catggagcca gaaaaggcag cgatgttact catacctgaa ccttggttat
     6781 ataatgaagc gaatgatgca aatgtacgtg cgttttatga attttatagt tttttaatgg
     6841 aaccgtggga tggtcctaca atgatttcgt tctgtaacgg tgacaaactt ggcgcgctta
     6901 cagatagaaa tggattacgt ccaggtcgtt atacgattac taaagataac tttattgtct
     6961 tttcatctga agtgggtgtt gtcgacgtac ctgaaagtaa tgttgctttt aaaggtcaat
     7021 tgaatcctgg aaagttattg cttgttgatt ttaaacagaa taaagtcatt gaaaataatg
     7081 atttaaaagg tgcgattgct ggagaattac catataaagc gtggattgat aaccataaag
     7141 ttgattttga ttttgaaaat atacaatatc aagattcgca atggaaagat gaaacgttat
     7201 ttaaattaca acgtcaattt gcatacacga aagaagagat tcataagtat attcaggaac
     7261 ttgtagaagg taagaaggat cctatcggtg caatgggata tgatgcgcca attgcagtgt
     7321 tgaacgagcg accagaatca ctatttaatt attttaaaca gctgtttgca caagttacga
     7381 atccaccaat tgatgcgtat cgtgaaaaaa tcgtaacgag tgaactttct tatttaggtg
     7441 gcgaaggtaa cttattagca cctgacgaaa cggttttaga tcgaattcaa ttgaagagac
     7501 cggtattgaa tgaatcacac ttagcagcga ttgatcagga gcattttaaa ttaacatatt
     7561 tatcaacggt atatgaaggg gatttggaag atgcgttaga agcattaggc cgagaagcag
     7621 tggatgctgt aaagcaaggc gctcaaattc tagtgttaga tgatagtgga ttagttgatg
     7681 gcaatggctt tgcaatgccg atgttactcg caataagtca tgtgcatcaa ttacttatta
     7741 aagcagattt acgtatgtct acaagtttag tcgctaaatc tggtgagaca cgagaagtgc
     7801 atcatgttgc ttgtttacta gcatatggcg cgaatgcaat tgtgccatac ctagcgcaac
     7861 gtacagttga acaactgaca ttgacagaag ggttacaagg caccgttgtc gataatgtta
     7921 agacatatac ggatgtattg tcggaaggtg tcattaaagt aatggcgaag atgggaattt
     7981 cgacagtgca aagttatcaa ggtgcacaaa tatttgaagc gattggcttg tctcatgatg
     8041 tgattgatcg ttattttact ggaacacagt ctaagttatc tggtatttcg attgatcaaa
     8101 tcgatgctga aaataaagca cgtcaacaaa gtgatgataa ttatcttgca tcaggtagta
     8161 cattccaatg gagacaacaa ggtcaacatc atgcttttaa tccggaatct attttcttat
     8221 tgcagcacgc atgtaaagaa aatgactatg cgcaatttaa agcatactct gaagcggtaa
     8281 acaaaaatag aacagatcat attagacatt tacttgaatt taaagcatgt acaccgattg
     8341 acatcgacca agttgaaccg gtaagtgaca ttgtcaaacg ctttaataca ggggcgatga
     8401 gttatggatc gatttcagca gaagcacatg aaacgttagc acaagccatg aaccaattag
     8461 gtggaaagag taatagtggt gaaggtggcg aagatgcaaa acgttatgaa gtacaagttg
     8521 atggaagcaa caaagtaagt gcgattaaac aagttgcttc tggacgtttt ggtgtaacta
     8581 gtgattattt acaacatgcc aaagaaattc aaattaaagt tgcgcaaggt gcaaagcctg
     8641 gtgaaggtgg tcaattacct ggtactaagg tatatccgtg gattgcgaag acaagagggt
     8701 caacgccagg tatcggtctg atttcaccac cgccacatca tgatatttat tcaatagaag
     8761 atttagcgca actgatacat gatttgaaaa atgcgaataa agatgcagat atcgcggtaa
     8821 aattagtttc gaaaacaggt gttggtacca ttgcatctgg ggtggcaaaa gcatttgcgg
     8881 ataaaattgt catcagtggt tacgatggtg gtacaggggc ttcacctaaa acgagtattc
     8941 agcatgccgg tgttccttgg gagattggtt tagcagaaac acatcaaaca ttaaaactaa
     9001 atgacttaag aagtcgtgtt aagttagaaa cagacggtaa gttattaact ggtaaagatg
     9061 tagcgtacgc atgtgcgctt ggagcggaag aatttggatt tgcaactgca ccattagtgg
     9121 tgttgggctg tattatgatg cgtgtatgcc ataaagatac atgtccagta ggagttgcaa
     9181 ctcaaaacaa agatttacgt gctttatata gaggtaaagc acatcatgtt gttaatttta
     9241 tgcattttat tgcacaagaa ttaagagaaa ttttagcatc tttaggtttg aaacgtttag
     9301 aagacttagt tggaagaact gatttattac aacgatcatc aacattaaaa gcgaatagca
     9361 aagcggctag tattgatgtt gaaaaactgt tatgtccttt cgatgggcca aacacaaaag
     9421 aaattcaaca aaatcataat cttgagcatg gatttgattt aacaaattta tatgagataa
     9481 cgaaaccata tattgctgaa ggacgtcgtt atacaggtag ctttacagta aataatgaac
     9541 aacgtgatgt aggggttatt acaggtagtg agatttcgaa acaatatgga gaagcaggac
     9601 ttcctgaaaa tacaattaat gtttatacga atggtcatgc tggtcaaagt cttgcagcat
     9661 atgcaccgaa aggcttaatg attcatcata ctggagatgc gaatgactat gttggtaaag
     9721 gattatctgg tggtacggtc attgtcaaag caccttttga agaacgacaa aatgaaatta
     9781 ttgctggtaa cgtctcattc tatggtgcga caggtggtaa ggcatttatt aacggtagtg
     9841 caggagaaag attctgtatt agaaatagtg gtgtagatgt tgtcgttgaa ggtatcggcg
     9901 accatggatt agagtacatg actggtggac atgtcattaa tttaggtgat gtaggtaaga
     9961 acttcggtca aggtatgagt ggtggtattg cttacgttat cccgtctgat gtagaagctt
    10021 ttgttgaaaa taatcaacta gatacgcttt cgtttacaaa gattaaacac caagaagaaa
    10081 aagcattcat taagcaaatg ctggaagaac atgtgtcaca tacgaatagt acgagagcga
    10141 ttcatgtgtt aaaacatttt gatcgcattg aagatgtcgt cgttaaagtt attcctaaag
    10201 attatcaatt aatgatgcaa aaaattcatt tgcaccaatc attacatgac aatgaagatg
    10261 aagcgatgtt agctgcattt tacgatgaca gtaaaacaat cgacgctaaa cataaaccag
    10321 ccgttgtgta ttaaggaaag ggggagatac gatgggcgaa tttaaaggat ttatgaagta
    10381 tgacaaacag tacttaggtg aattatcact ggtagaccgt ttgaagcatc ataaagcata
    10441 tcaacaacga tttactaaag aagatgcctc tatccaaggt gcacgatgta tggattgtgg
    10501 aacgccgttt tgtcaaaccg gacaacagta tggtagggaa acaataggtt gtccaattgg
    10561 aaactacatt cctgaatgga acgacttagt gtatcatcaa gattttaaaa ctgcttatga
    10621 acgcttaagc gaaacaaata actttcctga ctttacaggg cgtgtatgtc ctgcaccatg
    10681 cgaaagtgct tgtgtgatga agattaatag agaatcgatt gcgattaaag gtattgaacg
    10741 cacaattatt gatgaagctt ttgaaaatgg ttgggtagcg ccgaaagttc cgagtcgccg
    10801 tagagatgaa aaagtagcaa tcgttggaag cggtcctgca ggattaactg ctgctgaaga
    10861 acttaatcta ctagggtatc aagtaactat ttatgaacgt gctagagaat caggcggttt
    10921 attaatgtat ggtattccga atatgaagct tgataaagat gtggttcgac gtcgtattaa
    10981 gttaatggaa gaagcgggca ttgctttcat taatggtgtt gaagtaggcg ttgatattga
    11041 taaagcaacg ttagaatctg agtatgatgc cattatatta tgtactggtg cacaaaaagg
    11101 tagagattta cctttagaag gacgcatggg tgatggtata catttcgcta tggattattt
    11161 aactgaacaa acgcaactgt taaatggaga aattgatgat ataacaataa ctgcaaaaga
    11221 taagaatgtc attatcattg gtgctggtga tacaggggca gactgtgtag cgacagcatt
    11281 aagagaaaat tgtaaatcga ttgttcaatt taataaatat acgaaattgc cagaagcaat
    11341 tacatttaca gaaaatgcat catggccttt agcaatgccg gtgtttaaaa tggactatgc
    11401 gcaccaagag tacgaagcta agtttggtaa ggaaccacgt gcatatggtg ttcaaacaat
    11461 gcgttacgat gttgacgata aaggacaaat acgtggtttg tatactcaaa ttttagagca
    11521 aggcgaaaat ggtatggtca tgaaagaagg gcctgaaaga ttttggcctg ctgaccttgt
    11581 attattatca atcggcttcg aaggtacaga accaacagta ccgaatgctt ttaacattaa
    11641 aacggataga aatcgaatcg tggcggatga tacaaactat caaactaata atgaaaaggt
    11701 atttgctgct ggagatgcta gacgtggtca aagtttagtt gtatgggcga ttaaagaagg
    11761 tagaggcgta gcgaaagcag tagatcagta tttagcgagt aaagtttgtg tataatcttt
    11821 gtatggaaat ggtggttacg ttgacgttgt gtcatgctga atcgagtttg aaataatcta
    11881 gtatccatca acgtcacatg ccatctttgt aacctaaaaa caaaggtttg taagacaaca
    11941 aatagattaa ttataagtag tgattttttt aagtgcattt tataggtcaa ctgtagtgga
    12001 agacaatgat ttgtggtaat catgtaatgc ttaaaaacaa tattgacttt tacagaacgt
    12061 tcatatatga taaatattgt gttt
//
LOCUS       DAHHRR010000080        11842 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.080, whole genome shotgun sequence.
ACCESSION   DAHHRR010000080 DAHHRR010000000
VERSION     DAHHRR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11842
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.080"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            271..3960
                     /locus_tag="NBI42_001840"
     CDS             271..3960
                     /locus_tag="NBI42_001840"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCD1778252.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFIIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EDKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIESNEAILKRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            3950..5509
                     /gene="narH"
                     /locus_tag="NBI42_001841"
     CDS             3950..5509
                     /gene="narH"
                     /locus_tag="NBI42_001841"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCD1778253.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            5502..6077
                     /gene="narJ"
                     /locus_tag="NBI42_001842"
     CDS             5502..6077
                     /gene="narJ"
                     /locus_tag="NBI42_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCD1778254.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            6070..6747
                     /gene="narI"
                     /locus_tag="NBI42_001843"
     CDS             6070..6747
                     /gene="narI"
                     /locus_tag="NBI42_001843"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCD1778255.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAVGVSEHLYHIGAVYIGSIFGIITLV
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            6767..7219
                     /gene="nreA"
                     /locus_tag="NBI42_001844"
     CDS             6767..7219
                     /gene="nreA"
                     /locus_tag="NBI42_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HCD1778256.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDN"
     gene            7243..8277
                     /gene="nreB"
                     /locus_tag="NBI42_001845"
     CDS             7243..8277
                     /gene="nreB"
                     /locus_tag="NBI42_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HCD1778257.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            8300..8953
                     /gene="nreC"
                     /locus_tag="NBI42_001846"
     CDS             8300..8953
                     /gene="nreC"
                     /locus_tag="NBI42_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HCD1778258.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTSDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            9380..10549
                     /locus_tag="NBI42_001847"
     CDS             9380..10549
                     /locus_tag="NBI42_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HCD1778259.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRTYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            complement(10656..11084)
                     /locus_tag="NBI42_001848"
     CDS             complement(10656..11084)
                     /locus_tag="NBI42_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCD1778260.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKHKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNKTTSSTSIPIS"
     gene            complement(11300..11746)
                     /locus_tag="NBI42_001849"
     CDS             complement(11300..11746)
                     /locus_tag="NBI42_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1778261.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDRK"
BASE COUNT         4062 a         1743 c         2375 g         3662 t
ORIGIN      
        1 agaagttgac ggaaagtcaa cttacaataa tgtgcaagtt ggggtgggac aacgaaataa
       61 attttgtaaa aatataattt ctgtcccact cccatttcaa taaaattccg ttattgatag
      121 ggaaattcct atagttatta ggtaattccc taatatttta tgtttgtgaa aaaatgtaca
      181 atctaattga agcaatagtc ttgggcattt taaagtattt aaaaacacga catctacaca
      241 tcgtattttt atacgtagga ggatataaat atgggaaaat ttggattgaa tttctttaag
      301 ccaacagaaa aatttaatgg aaattggtcg atcctagaaa gtaaaagtag agaatgggaa
      361 aaaatgtaca gagaacgttg gagccacgat aaagaagtaa gaacaacaca tggtgttaac
      421 tgtacaggct catgttcttg gaaagtattt gtgaaaaatg gtgttattac ctgggaaaat
      481 caacaaactg actatccaag ttgtggtccg gatatgcctg aatatgaacc gagaggatgt
      541 ccacgaggtg cgtcgttctc ttggtatgaa tacagtccac ttcgaatcaa atatccatat
      601 attcgtggaa aactctggga tttatggact gaagcattag aagaaaacaa tggtaatcgc
      661 gttgctgcat gggcgtctat tgttgaaaat gaagacaaag ccaaacagta taagcaagcc
      721 cgaggtatgg gagggcacgt gcgttcaaat tggaaagacg ttacagagat aatcgcagca
      781 caattactgt atacaataaa aaaatatggt ccagatcgaa tcgcaggatt tacacctatt
      841 ccagcgatgt cgatgattag ttatgcagca ggtgctcgat tcatcaattt gcttggtggt
      901 gaaatgctta gtttttatga ctggtatgca gatttaccac ctgcctctcc acaaatttgg
      961 ggagagcaaa cagatgtgcc tgaatcaagt gactggtata acgcatcata cattattatg
     1021 tggggctcta atgtaccttt aacacgtact ccggatgcac attttatgac agaagtccgc
     1081 tataaaggta caaaagtcat ttcagtagca ccagattacg cagaaaatgt gaaatttgca
     1141 gataactggc tagcaccgaa tcctggttca gatgctgcaa ttgcacaagc aatgacgcat
     1201 gttattttac aagaacatta tgttaatcaa cctaatgaac gctttataaa ttacgctaaa
     1261 caatatacag atatgccgtt tattatcatg ctggatgaag atgaaaatgg atataaagcg
     1321 ggtcgatttt taagagcgag tgacttaggt caaacaacag agcaaggcga atggaagcca
     1381 gttattcatg atgcaatcag cgatagttta gtagtaccta atggcacaat gggtcaacgt
     1441 tgggaagaag ataagaagtg gaacttaaaa ctagaaacag aagatggttc taaaattaat
     1501 cctacattat caatgacaga aggtggatac gaattagaaa cgattcaatt cccatacttt
     1561 gatagtgatg gagatgggat attcaatcgt ccaattccaa ctcgacaagt cactttagca
     1621 aatggtgaca aagtccgtat tgctacaatt tttgacttaa tggcaagtca atatggcgtg
     1681 cgtcgttttg atcataaatt agaatcaaaa ggatacgacg atgcagaatc aaaatataca
     1741 cctgcttggc aagaagccat ttcaggcgta aaacaaagtg ttgtcattca agtagctaaa
     1801 gaatttgcgc aaaacgctat cgatactgaa gggcgttcaa tgattatcat gggtgcgggt
     1861 attaaccatt ggtttaactc agatacgatt tatcgttcaa tcttaaactt agttatgtta
     1921 tgtggctgtc aaggtgtgaa tggtggcggt tgggctcact atgtgggaca agaaaaatgt
     1981 cgcccgattg aaggatggag tactgtcgca tttgcgaaag actggcaagg accaccacgt
     2041 ttgcaaaacg gaacaagttg gttttatttt gcgacagacc aatggaaata tgaagagtca
     2101 aatgtagatc gtttaaaatc tccattagct aaaacagagg agttaaagca tcaacatcca
     2161 gctgattata atgttttagc agctagactt ggttggttac catcatatcc acaatttaat
     2221 aaaaatagtt tgttgtttgc agaagaagct aaagatgaag gcattgagtc gaatgaggca
     2281 attttaaaac gcgcgataga tgaagttaag tcaaaacaaa cgcaatttgc gatagaagat
     2341 cctgatttga aaaagaatca tccgaaatca ctgtttatat ggcgctcaaa tctaatctca
     2401 agttctgcaa aaggtcaaga atactttatg aagcatttac ttggcacaaa atcagggtta
     2461 ttagctacac caaatgaaga tgaaaagcca gaagaaatca cgtggcgtga ggaaacaaca
     2521 gggaaattag atttagtcgt ttctttagat ttcagaatga cagcaacacc tttatattct
     2581 gacattgtat tgccagcagc gacttggtat gagaagcatg atttgtcatc tacagatatg
     2641 catccatatg tacatccttt taatccagct attgatccat tatgggaatc gcgttcagac
     2701 tgggatattt ataaaacgtt ggcaaaagca ttttcagaaa tggcaaaaga ctatttacct
     2761 ggaacgttta aagatgttgt gacaacgcca cttagtcatg atactaagca agaaatttca
     2821 acaccatacg gcgtagtgaa agattggtcg aagggtgaaa ttgaagcggt acctggacgt
     2881 acaatgccta actttgcaat tgtagaacgc gactacacta aaatttacga caaatatgtc
     2941 acgcttggtc ctgtacttga aaaagggaaa gttggagcac atggtgtaag tttcggtgtc
     3001 agtgaacaat atgaagaatt aaaaagtatg ttaggtacgt ggagtgatac aaatgatgat
     3061 tctgtgagag cgaatcgtcc acgtattgat acagcacgta atgtagctga tgcaatacta
     3121 agtatttcat ctgctacgaa tggtaaatta tcacaaaaat catatgaaga tctcgaagaa
     3181 caaactggaa tgccgttaaa agatatttct agcgaacgcg ctgctgagaa aatttcgttt
     3241 ttaaatataa cttcacaacc acgagaagta ataccgacag cagtatttcc aggttcaaat
     3301 aaacaaggtc gacgatattc accatttaca acgaatatag aacgtctagt accttttaga
     3361 acattaacag gacgtcaaag ttattatgtg gatcacgaag ttttccaaca atttggggag
     3421 agcttaccag tatataaacc gacattgccg ccaatggtat ttggtaatag agataagaaa
     3481 attaaaggtg gtacagatgc tttggtacta cgttatttaa cgcctcatgg aaaatggaat
     3541 atacactcaa tgtatcaaga taataagcat atgttgacac tatttagagg tggtccaacg
     3601 gtttggatat caaatgaaga tgctgaaaaa cacgatatcc aagataatga ttggctagaa
     3661 gtgtataacc gtaatggtgt tgtaacggca agagcagtta tttcgcatcg tatgcctaaa
     3721 ggtacaatgt ttatgtatca tgcacaagat aaacatattc aaacgcctgg gtcagaaatt
     3781 acagatacac gtggtggttc acacaacgcg ccgactagaa tccatttgaa accaacacaa
     3841 ctagtcggag gatacgcaca aattagttat cactttaatt attatggacc aattgggaac
     3901 caaagggatt tatatgtagc agttagaaag atgaaggagg ttaattggct tgaagattaa
     3961 agcgcaagtt gcgatggtat taaatttaga taaatgcata ggatgccata cgtgtagtgt
     4021 gacatgtaaa aacacttgga caaatcgtcc aggtgctgag tacatgtggt tcaataacgt
     4081 agaaacgaag ccaggtgtag ggtatccgaa acgttgggaa gaccaagaac actacaaagg
     4141 tggttgggta ctaaatcgta aagggaaact tgaattaaaa tctggaagta gaatttcaaa
     4201 aattgcttta ggtaaaattt tttataaccc agatatgcca ttaattaaag attattatga
     4261 gccatggaac tataattatg aacatttaac aactgcgaaa tcagggaagc attcgccagt
     4321 tgctagagcg tattcagaaa ttacagggga taacattgaa attgaatggg gacctaactg
     4381 ggaagatgac ttagcaggtg gtcatgttac agggccaaaa gatcctaaca tccaaaaaat
     4441 agaagaagat attaaattcc aatttgacga aacttttatg atgtatttac cacgtttatg
     4501 tgaacattgc ttgaatccaa gttgcgttgc atcgtgtcct tcaggtgcta tgtataaacg
     4561 tgatgaagat ggcattgtat tagttgacca agacgcatgt cgtggatggc gttactgtat
     4621 gacaggttgt ccatataaga aagtttactt caattggaaa acgaacaaag ctgaaaagtg
     4681 tacattctgt ttcccgagaa tcgaggcagg tatgcctacg gtatgctctg aaacttgtac
     4741 aggacgtatg cgttacttag gtgttttatt atatgatgca gatcgagttc atgaagcagc
     4801 ttcagcagta gacgagaaag atttatacga aaaacaatta gatatattct taaatccatt
     4861 tgacgaagaa gtcattgctc aagctgaaaa agatggtatt ggttacgatt ggattgaagc
     4921 tgcacaaaac tctccaattt ataagttagc aattgaatat aaacttgcat tcccgttaca
     4981 tcctgaattt agaacgatgc cgatggtttg gtattgccca ccacttagcc caattatgag
     5041 ctattttgaa ggtaaaaata caactcaaaa tccagatgcg attttcccgg ccattgaaga
     5101 aatgcgttta ccaattgaat acttagctaa tattttcact gcaggagata cagaaccagt
     5161 aaaaggagcc ctacaacgta tggcaatgat gagaagttat atgaggtcac aagttactca
     5221 acagccattt gacacgtctc gattagagcg attaggtata acggagcgcc aaactaaaga
     5281 catgtatcgt ttattaggac tagccaaata tgaagatcga tttgttattc caacatcaca
     5341 caaagaaacc tatttagata cgtatcacgc acaaggtagt acaggataca attacggcgg
     5401 cgagcatttt ggagataact gtgaaggctg tggcgttgca gtaggttcag ggaaaactgg
     5461 tcaagaaatt tataatgaga atttctatgg agggattttc cgtgattaat ttcgataatt
     5521 ttaaaaaata tcaagaaagt tttggatata tggcacaaca gttatgtttt ccagaaaaat
     5581 taacttttca tccaaaaact tttgaagaaa caatttcgaa agatcatcca gcatatgaag
     5641 atttagtagc atttagaaat gtaatgatga cattttcatt atcagaaatc aaagcgattt
     5701 atacagatac atttgatttt acgaaaaaag caccattata tatgacgtat aacaaatttg
     5761 atacgcaaaa agaacgtggg caaatgttag ctaaattaaa ggtattatat gaaatgtttg
     5821 gactagaaat ggtagataat gaactttctg attatctacc gttgatgctt caatttttgc
     5881 aagtggcaga atggcgaaat gatccaagag ctgaagagaa tattcaacta atcattatga
     5941 tcattgagga tggaacatat gtgatggcga atgcacttgg tgaagataac aatccatatg
     6001 cctatgtgat tcaagcactt agaaaaacat tgaaattatg tttgacatca ccaaaaggag
     6061 tgaaacatca tgcttaatca gtttttatgg gttatatttc cgtatttatg tttagttgtc
     6121 tttgtcgcag gtcatattgc acgttatcgt tatgataaat tttcatggac agctaaatct
     6181 agtgaactaa tagaaagaaa aaggttgatg tggggaagtt tattatttca cttaggtatt
     6241 attccagtgt tttttggaca cgtagttgga ttgttaatac cgaaatcatg gatggatgca
     6301 gtgggtgtgt ctgagcattt atatcatata ggcgctgttt atattggtag catctttggt
     6361 ataataactt tggtagggat gtttttatta acagcgagac gtgttactac gaaaagtatt
     6421 cgaagattaa gttcagcgtc ggatatattt gtaaatttct tattattact tatcgtattt
     6481 atgggatgtt atgcaacatt agtaaccaat atacaacatc cagattttaa ctacagaact
     6541 tcattatcaa tttggtttag acaattattt atgttaaaac cagatgcgtc attgatgagt
     6601 ggtgtgccga ttgcatttaa aatgcatata ttgttaggtt tcactattat ggcttgttgg
     6661 ccatttacac gtttggtgca tgtttggagt gtacctttga catatataaa tcgcagatat
     6721 attatttacc gtaagaataa agtgtaagca taagaggtga aaggatttga caccagaagc
     6781 aataattgaa gatcgtagat ttcaagagac attagataaa atacgcaaag aagagggtta
     6841 tgattttgcg gcaatcgctt tctatgaatc taacaaacca tcatctccaa taaaatggca
     6901 ctatgtttca ggtaataaaa ataatcgatt taaattgatt attttaagaa aaggtcgtgg
     6961 attagctgga actgttatga aaacaggaaa gcgtatggta atagccaatg ttggtttagc
     7021 tttagggcct gaagaaaaga ttgactatcc aattttattg agcgaatcat taacagcagt
     7081 tttagcagtg ccgctttggt ataagaatca agtgtatggc gtactattat ttggtcaaag
     7141 agatggtcgt cctttaccaa aaatatttga taatgatgat attcaacgta agtttggtat
     7201 tttcaatgat gataattaat gagatgaatt agggtgtaag tcatgattaa tgaggacagt
     7261 atacagttag atactttatt gaaaaaatat tatgagcatt caatcgaaaa gatagttttt
     7321 gctgatgata acggcaaaat tattgcaatg aatgatgctg ctaaagatat attatctgaa
     7381 gaagataatt acagtgctgt ggcgaatgcg atttgtcaca gatgtgaagg gtatacaaat
     7441 gcctatgatg tacaatcatg taaagactgt ttcttagaat cgatgcaagt acaagctacg
     7501 aatttccaag tatttatgaa gacgaaagat caaaaagtga tgccatttac agcaacatat
     7561 cagttgattg atcaagacag gggaattcat gcatttacgc ttcaaaatgt ttcatcacaa
     7621 attgaacagc aagagaaatt acatcaacaa cgtatgatgc gtaaaacaat ctcagcccaa
     7681 gaaaatgaaa gaaagcgaat ttcaagagag ttacatgata gtgtgattca agaaatgctc
     7741 aatgtagatg ttcaattgag attattaaaa tatcaagaag atacaactaa gcttttggaa
     7801 gacgcagaaa atattgaata tattgtcgct aaattaattg atgatatacg taatatgtcg
     7861 gttgaattaa gacccgcttc tttggatgat ctaggccttg aagcagcttt taaatcatat
     7921 tttaaacagt ttgaagaaaa ttatggaata aaaatcatat atacatcaaa tattaaaaat
     7981 actcgctttg atagtgatat tgaaacggtt gtttatcgag tagtgcaaga agctattcta
     8041 aatgcgttga aatacgctga tgtaaatgaa ataaatgtag gtatccgtca aactgggcgc
     8101 catttggtag cagaagtgat agatgcggga aatggttttg atccaagttc taagcctaaa
     8161 ggttctggac tcggtttata tggaatgaat gagcgagctg agttagtcag cggtagcgtc
     8221 aatattgaaa cgaaaattgg tgagggtacc aatgttacat tgaacattcc aatttgaaac
     8281 atacattggg ggaataaaat tgaaaatagt cattgccgat gatcacgctg ttgtccgtac
     8341 agggttctct atgattttaa attatcaaaa tgatatggaa gttgttgcaa cggctgcaga
     8401 tggcgtcgaa gcttaccaaa aagtaatgga atataaacct gatgtgttac taatggattt
     8461 aagtatgcca ccaggtgagt caggtcttat cgctacgagt aaaattgctg acagctttcc
     8521 tgaaactaaa atactaatac taacaatgtt tgatgatgag gagtatttgt tccatgtatt
     8581 gcgtaatggt gcgaaaggtt acatattgaa aaatgcacct gatgaacaat tattgttagc
     8641 tattcgaact gtatataaag gtgaaacata tgtagatatg aaacttacaa catctttagt
     8701 gaatgaattt gtatctaatt caaatcagga cactgcaaac acatcagatc cttttaaaat
     8761 cttatcaaaa cgagaactag aaatattgcc acttattgcc aaaggttacg ggaataaaga
     8821 aattgcagag aaattatttg tatctgtgaa aacagtagaa gcacataaga cgcatattat
     8881 gacaaagctt ggcttaaaga gtaaacctga gctagttgaa tatgcattga aaaagaaatt
     8941 attagagttt tagagcttga aattaatata attcagtata aaagcgcgag aggaaatgat
     9001 tatcttctgg cgcttttata tgttttatca ttaaatatat gcagatagtt atgactaaga
     9061 attaggttga tgaatgggga ggatgtttga aattgaaaag tatgtgagtg ttcctaaata
     9121 atatgattaa aatgatggcg aataagtgtc taaaagcatc ttaaagggac attgtatagg
     9181 gtaaatcact tcataaataa gggaaaatcc ttatgttcac tttttcacaa tcataataaa
     9241 atatatatgt agtcaatact ttgtctatat tgaatgtttt catataaatg aaagcatttt
     9301 taaataacat tgacctctaa tatataggca gagtattgat atctattaaa aataaatgat
     9361 tttggtgaag gtgaaacgta tgtacaaaac aaaaggtggc tttcaactta cattacagac
     9421 attaagttta gtggttgggt ttatggcttg gagtataatt gcgccattaa tgccctttat
     9481 taaacaagat gtcaatgtta ctgaaggtca aatatcaatc attttagcga taccagttat
     9541 tttgggatcg gtgctccgtg tgccatttgg ttatctaaca aacattgttg gcgctaaatg
     9601 ggtattcttt actagtttta tcgtattgtt attcccgata tttttcttaa gccaagcaca
     9661 aacaccgggt atgttaatgg cgtcaggatt tttccttggc gtaggtggtg caattttctc
     9721 agttggtgtt acatcagttc ctaaatattt ccctaaagaa aaagtaggtc tagcaaatgg
     9781 tatttatggt atggggaata tcggtacagc agtttcttca tttttagcac cgccaattgc
     9841 gggtattatt ggttggcaaa caactgttag aacttactta attattatcg ctttatttgc
     9901 attaattatg tttatatttg gtgatacaca agaacgtaaa attaaagtgc cattaatggc
     9961 acaaatgaaa acattatcta aaaactacaa attatattac ttgagttatt ggtatttcat
    10021 tacttttggt gcttttgtag catttggtat tttcttacct aactacttag taaatcattt
    10081 tggaattgac aaagtagatg ctggtattcg atcaggtgta ttcattgcgc tggcaacatt
    10141 cttaagacca ataggtggca ttttaggtga taaatttaat gcagttaaag tattgatgat
    10201 tgattttgtt gttatgatta tcggtgccat tattttaggt atttcagacc atatcgcatt
    10261 attcactgta ggctgtttaa caataagtat ttgtgcaggt attggtaacg gcttaatctt
    10321 caaattagta ccatcatact tcttaaatga agcgggatcc gcaaatggta tcgtatcaat
    10381 gatgggtggt ttaggaggat tcttcccacc actagtaatc acatacgtag ctaatttaac
    10441 aggatcaagt catttagcat ttattttctt agcggtattt ggatgtattg cattatttac
    10501 catgcgtcat ttataccaaa aagaatatgg ctcattgaaa aacggttgat atgtaataca
    10561 tgacattcat ttagttaaat acaaagcctt aatatcatgc gcaatattcg tagcatgaca
    10621 ttaaggcttt agtagtgtat tcattttaaa tttaactatg aaattggaat agatgttgat
    10681 gaagtagttt tattgcttgg tttgatttta ggcaaggtaa tagttaataa cccattttca
    10741 aaactagcag taatatgttg cttatcaaca gcttcaaaat caaattgacg cattaatgat
    10801 tcgaagttac gctcatctaa aatgagttgt tcagatttgt gttttgcgct tctagtagct
    10861 tgaatagtga gcgtattatt attgaaatcg atactaatat cttctttatt tacacctgct
    10921 agttcagctt ctaagtaata taattcgtct gtttcatata tattcgttac aaatgaaggt
    10981 gttgaaaagt aattaaatac ttgtttacct aaatctttaa atgtgtcact tggattaccg
    11041 ttaaaaaagt tttgattctc gaattgattg aagttcaata tcatcacttc cattatataa
    11101 atttatatta caaagccatt atatgagtag tattaaaaaa gataaagtaa aaggggaggt
    11161 aaaaaagtta taaatgggta gatttgtgcc tgataaggaa tcagggcaaa agaactgagc
    11221 atcgagattt atacaatcaa taaagataat gaataaacat agcgatgcat acttctgccc
    11281 atcaccttat gatagggagt tactttctgt cggaatagtc aaaattttta gaaacaaaat
    11341 ttcgcaaatt attaattata tctgatgctt ctcgttctga aatgttgaat tcattaaaga
    11401 ccttaacaga aatttcagca agagggcttt ttattgcttt accttgttct gttagagaaa
    11461 tttgtaggtt tctttcatct ttctcttcac gtgttcgaac aacgtaatct ttcttttcta
    11521 atttctttag taatggtgtc agtgttccag aatctaagaa cacacgttca cctaattttt
    11581 tgatgttaag tttttcatca ttttcaatcg ccattaaaac aatgtaacca gtataggtta
    11641 aatcatattc cttaagatag tttgtgtatt ttttaattat ttcttttgaa gaaacataaa
    11701 ataaaaaaca caactgtttg ctaagatagc tgttttctac atacatccaa tcactccttg
    11761 ttaaaataaa caatattatg aagtgtaata gttgtcaagt actgatttca tcgcaattat
    11821 tgaattgtct ataattgaat tt
//
LOCUS       DAHHRR010000081        11772 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.081, whole genome shotgun sequence.
ACCESSION   DAHHRR010000081 DAHHRR010000000
VERSION     DAHHRR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.081"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..874
                     /locus_tag="NBI42_001850"
     CDS             <1..874
                     /locus_tag="NBI42_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCD1778262.1"
                     /translation="KIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKELQ
                     HVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDTMK
                     VKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESEIK
                     LADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITDIN
                     QTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDITNG
                     DKAFEKKLEKLEEK"
     gene            1028..1348
                     /gene="ytxJ"
                     /locus_tag="NBI42_001851"
     CDS             1028..1348
                     /gene="ytxJ"
                     /locus_tag="NBI42_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HCD1778263.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            1773..2897
                     /locus_tag="NBI42_001852"
     CDS             1773..2897
                     /locus_tag="NBI42_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD1778264.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIQVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMINHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLDENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPESYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(3085..4311)
                     /gene="pepT"
                     /locus_tag="NBI42_001853"
     CDS             complement(3085..4311)
                     /gene="pepT"
                     /locus_tag="NBI42_001853"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCD1778265.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDADVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENN"
     gene            complement(4325..4819)
                     /locus_tag="NBI42_001854"
     CDS             complement(4325..4819)
                     /locus_tag="NBI42_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCD1778266.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            complement(4837..5598)
                     /locus_tag="NBI42_001855"
     CDS             complement(4837..5598)
                     /locus_tag="NBI42_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCD1778267.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            complement(5794..6864)
                     /locus_tag="NBI42_001856"
     CDS             complement(5794..6864)
                     /locus_tag="NBI42_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCD1778268.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            7182..8237
                     /locus_tag="NBI42_001857"
     CDS             7182..8237
                     /locus_tag="NBI42_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCD1778269.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            complement(8409..9050)
                     /locus_tag="NBI42_001858"
     CDS             complement(8409..9050)
                     /locus_tag="NBI42_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCD1778270.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAIPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            9194..10060
                     /gene="fakB1"
                     /locus_tag="NBI42_001859"
     CDS             9194..10060
                     /gene="fakB1"
                     /locus_tag="NBI42_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HCD1778271.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            10424..11506
                     /locus_tag="NBI42_001860"
     CDS             10424..11506
                     /locus_tag="NBI42_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1778272.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRM
                     RQTILLYAVTGAGKTEMMFQGIQYARRQGDNIAIVSPRVDVVVEICKRIKDAFLNEDI
                     DILHQQSSQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKSLQQALKSSSK
                     VKHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNNKIQKMLYR
                     ILQDQINKQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQKMNKLALKRGWIDE"
     gene            11499..>11772
                     /locus_tag="NBI42_001861"
     CDS             11499..>11772
                     /locus_tag="NBI42_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCD1778273.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMI"
BASE COUNT         4156 a         1698 c         2032 g         3886 t
ORIGIN      
        1 aaaaattgtt attatcgctg ttttagcgat tttatttgta gtaataagtg cttgtggtaa
       61 taaagaaaaa gaggcacaac atcaatttac taagcaattt aaagatgttg agcaaaaaca
      121 aaaagaatta caacatgtca tggataatat acatttgaaa gaaattgatc atctaagtaa
      181 aactgataca actgataaaa atagtaaaga atttaaggca ctacaagaag atgttaaaaa
      241 ccatctcata cctaaatttg aagcatatta taagtcagca aaaaatttgc ccgatgatac
      301 aatgaaagtt aagaaattaa aaaaagaata tatgacgctt gcaaatgaga agaaggatgc
      361 gatatatcaa ttaaaaaaat tcataggttt atgtaatcaa tctatcaagt ataacgaaga
      421 cattttagat tatacgaaac aatttgaaaa aaatagatac aaagttgaat cagaaattaa
      481 attagctgat aataaaagtg aagcaacaaa tcttacgacc aaattagaac ataataataa
      541 agcgttaaga gatactgcga aaaagaacct agatgatagt aaagaaaatg aagtaaaagg
      601 cgcgattaaa aatcacatta tgccaatgat tgaaaaacaa attaccgata ttaaccaaac
      661 taatattagt gataagcatg ttaataatgc aaggaaaaac gcaatagaaa tgtattacag
      721 tctgcagaac tattataata cacgtattga aacaataaag gttagtgaga agttatcaaa
      781 agtcgatgta gataagttgc cgaaaaaggg tatagatata actaacggcg ataaagcctt
      841 tgaaaaaaag cttgaaaaat tagaagaaaa ataactataa tcatttttca aagttaaaaa
      901 ttttgaattt atggttaaca tgtcaactta ctatgtgtat aatggtaaac attgatatta
      961 actatatgta taaaaatgtc acgcagatgc tatttaaatg tgataaatat ttttagaggt
     1021 gaatagagtg gctataaagc taagttcaat tgaccaattt gaacaggtta ttgaggaaaa
     1081 taaatatgtt tttgtattaa aacatagtga aacttgtcca atatcggcaa atgcgtacga
     1141 tcaatttaat aaatttttat atgaacgcga tatggacggt tattatttga ttgtccaaca
     1201 agaacgcgat ttgtcagatt atattgctaa aaaaacgaac gttaaacatg aatcacctca
     1261 agcattttat tttgtaaatg gtgaaatggt ttggaataga gaccacggtg atattaatgt
     1321 gtcgtcatta gcacaagcag aagaataatg aaactatagg gttggaacat tttgccttac
     1381 actactagac gtgaatagca caacttaaat tcgtgtgaat cagagtagtt tggctataat
     1441 aatgttctga ccttttattt tatataacct ttagaagcag ttaagttagt acttttttac
     1501 aaacatatgt ataatgtatt cgagtatttt tattggaaat attttggaaa acgacgaatc
     1561 caataagaaa atttaaacat gatttgtaag ttagtttaat aggaaatata tgctaaacca
     1621 gaagaagcat actgttattt actggaataa ttaataatca tgtcatgtta aatgttcgca
     1681 tataatcacg agataaaatc taaaatttaa gattaatctt ttatgaataa aaaacgtatc
     1741 acaacaaata atagagtagg gtggtcaagg ttatgaaagt attagtagcc atggatgagt
     1801 ttcatggaat tatttcgagt tatcaagcta atagatatgt tgaagaggca gttgcaagcc
     1861 aaattgaaac tgcagatgta gttcaagtac cattgtttaa cggaagacat gaattattag
     1921 attctgtatt tttatggcaa tctgggcaaa agtatcgtat acaagtacat gatgcagata
     1981 tgaatgaagt tgaaggtgtt tacggacaaa ctgatacagg gatgaccgtt atcgagggga
     2041 atttattttt aaaaggtaaa aagccaattg ttgaacgaac aagttatggt ttaggagaaa
     2101 tgattaacca tgcattagat aacgacgcaa aacatgttgt aatttcatta ggtgggattg
     2161 atagttttga tgctggtgca ggtatgttac aagcattagg tgctcaattc tatgatgacg
     2221 aaggacgtgt cgtagatatg agacaaggtg ctggtgtaat taaatatatt cgtcgtatgg
     2281 atatgtcgaa cttacaccct aaaatggaaa cagcaagaat tcaagtaatg tcggattttt
     2341 caagtcgatt atatggtaag caaagtgaaa tcatgcaaac ttatgatgcg catcagttga
     2401 atcataatca agcagcagaa atcgataatt taatttggta ttttagtgag ttatttaaaa
     2461 gtgaattgaa aattgcaatt gggccagttg aacgtggtgg tgctggtggt ggaattgcag
     2521 cagtcttgaa tggactgtat caagctgaaa tattaaccag tcatgcatta gtagaccaac
     2581 taacacattt agaaaattta gttgaacaag cggatttaat tatttttgga gaaggattag
     2641 atgaaaatga tcagttgcta gaaacgacaa cattgcgtat tgcagaactt tgtcataaac
     2701 atcaaaaggt agccattgca atttgtgcaa ctgctgaaaa gtttgattta tttgaatcac
     2761 aaggggttac agcaatgttt aataccttta tagatatgcc ggaaagttat actgacttta
     2821 aaatggggtt acaaattagg cattatactg ttcagtcttt aaaactgttg aaaacacatt
     2881 ttaatgttga ggtatagtaa agaaggacta aattggtgat gctgtcatga tggttaataa
     2941 catttatgat ggttagcaaa acaaattaga agatcgaaag tatacgtaaa aaatatgaaa
     3001 aatcacgcta tcattgcact gaatgttagc gtgatttttt atattaatta agcctgagtt
     3061 gaactagtat ataatcgttt gtttttagtt attttcagcg atatcttcta caattccaat
     3121 gattacttgt actgcctttt ccatgacatc aatggatgca tattcatatg ggccgtgaaa
     3181 gttaccgcaa cctgtaaaga tgtttggagt tggtaacccc ataaatgaca attgtgaacc
     3241 atctgtacca ccacgaatag gttcagtgtt tgctggaata tctaatttag caaagacacg
     3301 tttaggtata tcaataatat gaggcaatgg taatattttt tctgccatat tgaaatattg
     3361 atccgatata tcaactttaa ctggataatt ttcaaaatgt gcattgatat cgtcacgtat
     3421 ttctaaaata cgtttcttac gcaattcgaa ttgtttttta tcatgatcac gaataatgta
     3481 ttgcaaagtt gctttttcaa cagttccttc aaagttcatt aagtgataaa agccttcgta
     3541 tccttctgtg cgctccggaa cttcactatc aggtagcaaa ctatcgaatt gttcacctaa
     3601 acgtattgcg tttaccattg catttttagc tgaaccagga tgaacattca caccgtggca
     3661 tgtaataacc gcctcagcag cgttaaagct ttcatattgt aattctccat attgactacc
     3721 atccatagta taagcaaaat cagcattgaa gcggtcaaca tcaaatttat gtggaccacg
     3781 accgatttct tcgtctggtg taaatccaat gcgaatggta ccatgtttaa tttctggatg
     3841 ttcttgtaaa taacaaatag cttccataat ttccacaata cccgctttat cgtctgcacc
     3901 tagtaacgat gtaccatcag ttaccattaa tgtatgacca actaaactgt taagttctgg
     3961 aaatacttta ggatctaaga cacgtttagt attgcctagt ttgtatggct taccatcata
     4021 gttttcaata atttgcggtt taacatttga agcattgaaa tcaggtgatg tatcaacatg
     4081 ggccaaaaat ccaactgttg ggacgtcggc atcgatgtta ctttctaatg tagcaaataa
     4141 gtagccattt tcatctaaat cagttggcaa tcctaattgt tgtaattctt tttctaataa
     4201 atgtaacaaa tcccattgct tttcagttga aggtgttgtt gtagattttg gatcagattg
     4261 cgtatcaatt gtcgtatacc ttgttaatct atctatcaat tggttcttca ttatattcga
     4321 ccccttaaac tctattattc atgttgtaag attttttata tgttttacct ttgattttac
     4381 catacagttg tttgatacgt gtgtacaggt aatatagaat ttcagaaact agtataccga
     4441 aagcaatcgc gcctgaaatc agtgtaactt ctaaaaatgt atttacagca cttgtataat
     4501 cattcgatac taaaaaacga gtcgcttgat aagctgcacc accaggtact aatggtataa
     4561 tgcctggcac tatgaatata attaccggtc gtttatatct gcgactcata gtatgactca
     4621 ttaagcctaa aattaagctt cccaaaaatg aagcgccaac ttttccaaac tctaaatcta
     4681 ccgttaattg gtaaatcgtc catgcaatgg cacctacaaa tccacatgct actaagagac
     4741 gtttgggtgc attgaaaatg atagagaaaa gtactgttga tataaagctg attgtaaaat
     4801 gaaataaata aaatagcatg ctttaacagt ccttccttaa atgattaata aaacgattgc
     4861 gacaccagca ccgattgcga atgctgttaa tgcagcttca acacctcgag acatacctgc
     4921 aagtaattca cccgctaata aatctcgaat ggcattggta attaatatac cagggacaag
     4981 tggcatgaca ctggctatag taatgatatc ttgattggtt gcaataccta atttagtaaa
     5041 tgtggctgca atggatatga ccacagcggc tgcaacaaac tctgagaaaa atttaatttg
     5101 tatatagcgt tgcacgaagc tgaatgttaa aaatgcggat ccaccagcaa tgactgcaat
     5161 ccaacaatct gatgcgacac caccaaacat aaataggaag aagccacatg caatggcagc
     5221 tgcaaagaaa ttcgttaaaa aagaatattg taatgatgca tgctgtaaat gaataaattc
     5281 agatttagct tcatcaattg tgagttcttt atttgatatt ttacgtgaaa gactattcgt
     5341 taaagcgatt ttctctaaat ctgttgtacg ctcttgtaca cgaattaatc ttgtacttgt
     5401 tcgatcgttt aatgaaaaaa taattgcagt tgaactgaca aaactatatg tattatgaag
     5461 accataacta tgtgcgatac ggttcattgt atcttcaact cgatatgttt cagcacctga
     5521 ttcaagtaaa attctacctg caattaatac aacatcaatc actttgtttt catctataat
     5581 tgtgattgaa tctggcatat caattcacct ccaatgatat gtgttattta tttgaacaat
     5641 tgaagtttac aacttgttgt tgcaactttc aatagtgaga ctttgtgtta gtatgatgaa
     5701 cttgtatggt tcaaatttaa ataagaaaaa ctgttaatct ttgctattat actatgattt
     5761 aataatagca aaggattaac agttttgtcg ttgttataaa ttgataatag ggttaaacat
     5821 tactttgttt cgcccttgat ttttggctac atgcaccata tcgtctgcat ctttaaacac
     5881 tttacgctgt gattttggat cgtcatctgt tagataacca acaccgatag acactgacaa
     5941 tttaataact tctttgtttg gtaaatggaa tgatgatttt tcaacacctg aacgaatatt
     6001 ttcagctaat ttaacacttt gatcaagtga atagttgtga atgacaactg agaactcttc
     6061 gccaccattt ctaaaaattt taaattgatt cggcacatag tttttaagta attgagacat
     6121 ttgttttaat acagcatcac ctgatttgtg tgagtaggta tcattgacat ctttaaatcc
     6181 atcgatatcg attaataata atgcgatact ttgatgttct ttttcagctt ttcgtgaaat
     6241 ttcatttaaa tgtctatcaa attcttttac attacctaag cctgttaagt aatcatattt
     6301 atcttcgttt tcataacgat ttacgagtga gaagaaatgc caaatatcga caaatgttat
     6361 cgctgaagct aaagtgataa ttaatgaaat tggtattaaa atgataactt ccgatagtgt
     6421 gtaaatagga ctcactaatg cgacaccaaa taaaatgatt attgtaacaa cattaagtat
     6481 taataatgat agcacatcat tttgttttaa aaatggtcca atagcacttg ttactgcggc
     6541 aataacaatc aacgtaacac cgtacataat cgagttgtta aatactacaa tttcaacaat
     6601 tgctacaatt actgtggcag ataatgtata gaccatattt gtaaatctac ctaaaaacaa
     6661 taaaggaacg aatgttaagt gaattaaata atcttcacga taagggatag ggtagacaga
     6721 taataataat gatacgattg tcattaaaac agtgacataa gccttagaaa aaaccatacg
     6781 tttgttttct gaatactgta agcggtggaa taaatagatt ccagcgacta taacagatat
     6841 attgtatata aatgcttcga acatgtctga atcgactcct ttaattgacc actagctatt
     6901 gtaagtgaaa acttacaatt tgtcattagt ttacatataa aattaatgta tgatatagac
     6961 tttgatgtta aaatgttgcc ttaaatgata tgatgaaaaa atgaataata ggcgatatat
     7021 aagaaatgaa tcgtatagtt gtaaatatga tatcattgat tgagcgaatt aatttataat
     7081 aaagctataa gatatacgta gaaaatagat atatcattct ataaagacaa tattaaataa
     7141 atataacgtt aaaaacaatt aatatcgatg aaggtgaata aatggttaca ttattactag
     7201 ttgcagtaac aatgattgtc agtttgacga taacaccaat tgttattgca atatcgaaaa
     7261 gattaaattt agttgataaa ccaaatttta gaaaagtaca cactaaacct atttcagtta
     7321 tgggtggtac agtgattctc ttttcatttt taataggtat ttggattggt catcctattg
     7381 aaacagaaat caaaccactt attattggtg cgattattat gtacgtactt gggcttgtag
     7441 atgatatcta cgatttgaaa ccgtatataa aattggctgg tcaaattgcc gctgccttag
     7501 tagttgcttt ttatggtgtg actattgatt ttatttcgtt gccaatgggt acaacgattc
     7561 attttggatt tcttagtatt ccaattactg tgatttggat tgttgctatt acaaatgcaa
     7621 ttaacttaat tgatggactc gatggtttgg cgtcgggtgt ttctgcaata gggctcatta
     7681 caataggatt cattgcaatt ttacaagcta atattttcat aacgatgatt tgttgtgttt
     7741 tattaggctc tttaattggg tttttatttt acaatttcca tcctgccaaa atatttttag
     7801 gtgatagtgg ggctttaatg attggattta tcatcggatt cctttcttta ctcggattca
     7861 aaaatattac aattattgca ttgttcttcc caattgttat cctagcagtt ccattcattg
     7921 atactttgtt cgcaatgatt cgacgtgtga aaaaagggca gcatataatg caagctgata
     7981 aatcgcattt gcatcataaa ctattagctt taggctacac acatagacaa acagtattat
     8041 taatctattc aatctctatt ttatttagtc tttcgagcat tatcttgtat gtatcgccac
     8101 cattaggtgt ggtattaatg tttgtattaa tcatatttag tattgaatta attgttgaat
     8161 ttacaggatt aatagataac aactatcgac caatattaaa tttaattagt cgtaagtcat
     8221 ctcataaaga ggaataggga atgaaagcat agttgtatgg gataatttgt attatatggc
     8281 tttactcttt acaatttttt atgtattaaa tttcaaatat aaaaaagcac tgccataaac
     8341 gtgtacttca attgtcattt aataatacgt aattgatatt taccgtctta tgatagtgct
     8401 ttttattttt attcagttgg tatatcgaaa ggtaactgct ttggagtttc ttcagtcaaa
     8461 tcgaaatttc cagcagtcat ttgatttaaa aagttaataa acgcttcata gtcactttta
     8521 acgacgtcga tatagtaact taccttatct gtgtaagttt ggtttcttaa cataaaatga
     8581 gttgaagcta attcatattc aaatttacca gtttgatcat aattcagtgt tactatacat
     8641 ggtattgctt ctcgtagttc gacacgtccg atatcataaa tgacgtctct aacagcaccg
     8701 ctataggcgc gaattaaacc gccaccacct aatttaatac caccaaaata tcttgttact
     8761 acgacacacg cattatgaac atcgagcttt tttaatatgt ctaacattgg gacaccggca
     8821 gttcctgtcg gttcaccatc atcattcgct ttttgaatat tcatttcagg tccaatagta
     8881 tatgcagaac aattatgagt ggcatcttta tgttcttttt ttattgcagc aataaatgct
     8941 ttagcttcat cttcattttg aacaggtttg atatgagcaa tgaatcttga tttactaatc
     9001 acattttcaa taatgtgttc ttttttaaca gtaatgatat tttgtgtcat aataactcct
     9061 taattcataa gcttaagatt atttaatctt cattatacac tgaaaatgac atgactataa
     9121 atcgtttgat tgtcattttc tttttaactg aaatattgta tcattgctat gagtatattt
     9181 taggaggacg actatgaaaa ttgctgtgat gaccgattct acaagttatc tgtcgcagga
     9241 cttaatcgat aaatataata ttcaaatagc gccattaagt gtgacttttg atgatgggaa
     9301 gaactttaca gaaagtaatg aaatagcaat tgaagaattt tataataaaa tggcatcgtc
     9361 tcaaacgatt ccaacaacaa gccaaccagc aattggcgaa tggattacta aatatgaaat
     9421 gttaagagat caaggttaca cagatatcat tgtcatttgc ttatcaagtg gaattagtgg
     9481 aagttatcaa tctagttatc aagcagggga aatggttgaa ggtgttaatg tacatgcatt
     9541 tgatagtaag cttgcagcaa tgattgaagg atgctatgta ttacgtgcta ttgaaatggt
     9601 tgaagaagga tacgaaccac agcaaattat tgatgattta actaatatgc gtgaacacac
     9661 aggtgcatat ttaattgttg atgacttaaa gaatttacaa aaaagtggtc gaattactgg
     9721 tgctcaagca tgggttggaa cattattgaa aatgaagcca gttcttaagt ttgaagatgg
     9781 caagattata ccagaagaaa aagttcgtac taaaaagcgt gccattcaaa cattagaaaa
     9841 gaaagtatta gatattgtaa aagactttga agaagtaact ttatttgtca taaatggaga
     9901 tcatttcgaa gatggtcaag cgttatacaa aaagttacaa gatgattgtc cttcagctta
     9961 tcaagtagca tactctgagt ttggtccagt tgttgcagca catttaggtt ctggtggatt
    10021 aggtttaggc tatgttggca gaaaaataag attaacataa ttataaaatt ttaataaaag
    10081 agtctatatt gtaattggaa attatctctc gtatacatgg ctttaaatgt tcatcatttg
    10141 aaagtcaaaa tgctaaagat ataagaaaat cattataata ttaggctctt ttttacgttg
    10201 aaatgaggtt ttaagcatta agcattacgg gaaattaatt catcctcata cttcacttac
    10261 taatgaaaaa attaaaaaag aagtaacagg tgtcatcaaa caaaattcaa actattattg
    10321 tgttcaatgt gaaagtacaa atccaaagca tttttatcag tatgattcct cagtacattc
    10381 caagaaaatt gtatattgca gaaattgtat atcactgggt cgaatggata atgtaacaag
    10441 atataaaata acagagagtt cgcaaagttc atcacaagca tattatcatc tctcatttga
    10501 attgtcggaa cagcagtctt atgcctcaga acatattgtt cgagccatta gaatgagaca
    10561 aacgattttg ttatatgcag taacaggtgc aggtaagaca gaaatgatgt ttcaaggcat
    10621 tcaatatgca agacgacagg gggataatat agctattgtg tcaccacgtg tagatgttgt
    10681 tgtagaaatt tgtaaacgta ttaaagacgc atttcttaat gaagatatag acatactaca
    10741 ccagcaatca agtcaacaat ttgaagggca ttttgttgta tgcacagtgc atcaacttta
    10801 ccgattcaaa cagcactttg atactatttt tattgatgaa gtcgatgcct ttcctttatc
    10861 aatggataaa agtttacaac aagcattgaa gtcatcttct aaagttaaac atgcaacgat
    10921 ttatatgaca gcaacaccac cgaaacaact tctgtcagag attccccacg aaaatataat
    10981 taaattgcca gctcgctttc ataaaaaatc acttccagtt cctaaatatc gctatttcaa
    11041 acttaataat aataagattc agaaaatgtt atatcgaatt ttacaagatc aaattaataa
    11101 acaacgttat acactggtgt tttttaacaa tatagaaaca atgattaaaa cattttcggt
    11161 ttataagcag aaaattacta aattaacata tgtccatagc gaggatgttt ttcgctttga
    11221 aaaagttgaa caattaagga atggacattt cgacgtcatt tttactacga caatattaga
    11281 acgtggattt acaatggcaa atttggatgt tgttgttatc gatgcacatc aatatactca
    11341 agaggcttta atacaaattg ctggacgtgt tggacgaaaa ttagaatgtc ctacaggaaa
    11401 agtattgttt tttcatgaag gagtaagtat gaatatgatt caagctaaaa aagagattca
    11461 aaagatgaac aaattagcat taaaaagagg ttggattgat gaataattgt ttgagttgtg
    11521 gtgctaagtt atatgaaaat ataaccattt ataatttgtt caagaaacct aatagattat
    11581 gtgacagatg caaagagaat tgggacaata ttaaacttga tattaaagca aggcgatgtt
    11641 caaggtgctt aaaacactta aatcaagatg aagcgtattg tttagactgc aagtttctat
    11701 cggcacactt taatttaatg gaacaattat attgtcaatt tcaatatgac ggtttaatga
    11761 aagagatgat ac
//
LOCUS       DAHHRR010000082        11634 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.082, whole genome shotgun sequence.
ACCESSION   DAHHRR010000082 DAHHRR010000000
VERSION     DAHHRR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11634
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.082"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            214..1098
                     /gene="ylqF"
                     /locus_tag="NBI42_001862"
     CDS             214..1098
                     /gene="ylqF"
                     /locus_tag="NBI42_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCD1778274.1"
                     /translation="MVIQWYPGHMAKAEREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     STAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKAHYKIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            1082..1849
                     /locus_tag="NBI42_001863"
     CDS             1082..1849
                     /locus_tag="NBI42_001863"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCD1778275.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKENPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAFGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            1958..3124
                     /gene="sucC"
                     /locus_tag="NBI42_001864"
     CDS             1958..3124
                     /gene="sucC"
                     /locus_tag="NBI42_001864"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCD1778276.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            3146..4054
                     /gene="sucD"
                     /locus_tag="NBI42_001865"
     CDS             3146..4054
                     /gene="sucD"
                     /locus_tag="NBI42_001865"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCD1778277.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPALFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNICGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            4281..5399
                     /locus_tag="NBI42_001866"
     CDS             4281..5399
                     /locus_tag="NBI42_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD1778278.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEAVVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVVNNNEPTKEIKIANLEAQNSNQKKVNKGTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNNSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
     gene            5427..6671
                     /gene="fmhC"
                     /locus_tag="NBI42_001867"
     CDS             5427..6671
                     /gene="fmhC"
                     /locus_tag="NBI42_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCD1778279.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KDDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGV
                     FILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVL
                     DLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEDKHGFHF
                     MNEDYFKRMQEIYKDKAMLKIACIDLNEYQDKLKIQLLKIENEMMTVNRALNENPNSK
                     KNKSKLNQLNMQLSSINNRISKTEELILEDGPVLDLAAALFICTDDEVYYLSSGSNPK
                     YNQYMGAYHLQWHMIKYAKSHNINRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEEL
                     IGDFIKPVRPILYKFAKLIYKV"
     gene            6843..7715
                     /gene="dprA"
                     /locus_tag="NBI42_001868"
     CDS             6843..7715
                     /gene="dprA"
                     /locus_tag="NBI42_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCD1778280.1"
                     /translation="MIRLFLLKLYWAHFSTKQIHQFLMAYPNVIKEGGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDTEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEIGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            7889..9964
                     /gene="topA"
                     /locus_tag="NBI42_001869"
     CDS             7889..9964
                     /gene="topA"
                     /locus_tag="NBI42_001869"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCD1778281.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            10120..11427
                     /gene="trmFO"
                     /locus_tag="NBI42_001870"
     CDS             10120..11427
                     /gene="trmFO"
                     /locus_tag="NBI42_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HCD1778282.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITEVLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
BASE COUNT         4475 a         1545 c         2207 g         3407 t
ORIGIN      
        1 aatatcacct aataagcagc ttagatttat taaatgaaaa tttttaatta tagaaaactt
       61 ggattaatca cttatacctt aatctaaagc attgttaaga agtgtgacaa tgttaaaata
      121 aatatagttg aattaatgaa tttgttctat aattaacaag ttattaattt taagaaggta
      181 aagaaaagaa ctgacgaaag taaggtgaat tgaatggtta ttcaatggta tccaggacat
      241 atggcgaagg ccgaaagaga agtaagtgaa caattaaaaa aagtagatgt agtatttgaa
      301 cttgtagatg caagaattcc ttatagttca aggaatccaa tgatagatga agttattaac
      361 caaaaaccac gtgttgttat attaaataaa aaagatatgt ctaatttaaa tgagatgtca
      421 aaatgggaac aattttttat tgataaagga tactatcctg tatcagtgga tgctaagcac
      481 ggtaaaaatt taaagaaagt ggaagctgca gcaattaagt cgactgctga aaaatttgaa
      541 cgcgaaaaag cgaaaggact taaaccacgt gcaattagag cgatgatagt tggaatacca
      601 aatgtcggga aatcaacttt aataaataaa ctggcaaagc gtagtatcgc acagactggt
      661 aataaaccag gtgtgaccaa acaacaacaa tggattaaag ttggtaatgc attacaatta
      721 ttagacacac cagggatact ttggcctaaa tttgaagatg aagaagtcgg taagaagttg
      781 agtttaacag gagcgattaa agatagtatt gtgcacttag atgaagttgc catctatggt
      841 ctgaactttt taattcaaaa tgatttagcg cgcttaaaag cacattataa aatcgaagta
      901 cctgaagatg cagaaatcat agcgtggttt gatgcgatag ggaaaaaacg tggcttaatt
      961 cgacgtggta atgaaattga ttacgaagca gtcattgaac tgattattta tgatattcga
     1021 aatgctaaaa taggaaatta ttgttttgat atttttaaag atatgactga ggaattagca
     1081 aatgacgcta acaattaaag aagtaacgca gttgattaat gcggttaata caatagaaga
     1141 attagaaaat catgaatgct ttttagatga gcgaaaaggt gtacaaaatg ccatagccag
     1201 acgaagaaaa gcgttagaaa aagaacaagc tttaaaagaa aaatatgttg aaatgactta
     1261 ctttgaaaat gaaatattaa aagagaatcc taatgctatt atttgtggta ttgatgaagt
     1321 tggaagggga cctttagcag gtccagtcgt tgcatgcgca acaattttaa attcaaatca
     1381 caattatttg ggccttgatg attcgaaaaa agtacctgtt acgaaacgtc tagaattaaa
     1441 tgaagcacta aaaaatgaag ttactgcttt tgcatttggt atcgcgacag ctgaagaaat
     1501 agatgaattt aatatttata aagctactca aatcgccatg cagcgagcta ttgatggatt
     1561 atcagtacaa ccaacgcatt tattgataga tgcgatgacg cttgataatg cactgcctca
     1621 agtatcttta atcaagggtg atgcaagaag tgtatctatt gcagctgcaa gtatcatggc
     1681 aaaggttttt cgtgatgatt atatgacaca gttatctaaa gattatcctg aatatggttt
     1741 tgaaaaaaac gcgggttacg gtaccaaaca acatttacta gcaatcgatg atattggcat
     1801 tatgaaagag catagaaaaa gctttgaacc tataaaatcg ttactgtaaa tcgtaaaatt
     1861 caattaaatg ataaaaaagt agcatttaag tttacaatag cgcttacatt ttttataatt
     1921 gtaagggaac ttaacctaag taacaggagg atggaagatg aatatccacg agtatcaagg
     1981 taaagaaata tttcgttcaa tgggcgttgc agttccagaa ggacgagtag catttactgc
     2041 tgaagaagcg gtggagaaag caaaagaatt aaattctgat gtatatgttg taaaagcaca
     2101 aattcatgct ggaggtagag gtaaagcagg cggagtaaaa attgctaaat ctttatctga
     2161 ggtagaaaca tatgcaaaag aattattagg gaaaactttg gtgacacatc aaactggtcc
     2221 agaaggtaaa gaaattaagc gtttatatat cgaagaaggt tgtgctattc aaaaagaata
     2281 ttacgttgga ttcgttattg atcgtgcgac tgaccaagta acattgatgg cgtctgaaga
     2341 agggggcact gagattgaag aagttgctgc gaagactcct gaaaagatct tcaaagaaac
     2401 tatcgatcca gtaatcggac tttcaccatt ccaagcaaga cgaattgcgt ttaatattaa
     2461 tattcctaaa gaatctgtta acaaagctgc taaattctta ttagcacttt ataatgtatt
     2521 cattgaaaaa gattgttcaa tcgtagaaat caacccatta gttacaacag ctgatggtga
     2581 tgtattggca ttagatgcta aaattaattt tgatgataat gcattattca gacataaaga
     2641 tgttgtagaa ttacgtgatt tagaagaaga agatccgaaa gagattgaag cgtctaaaca
     2701 tgatttgtca tacattgcat tagatggtga catcggatgt atggtgaatg gtgcaggttt
     2761 agccatggca acaatggata cgattaatca tttcggtgga aacccagcca atttcttaga
     2821 tgcaggcgga agcgctacta gagaaaaagt aactgaagca tttaaaatca ttttaggtga
     2881 tgaaaatgtt aaaggtattt ttgtaaacat tttcggtggc attatgaaat gtgatgttat
     2941 cgcagaaggt atcgttgaag ctgtaaaaga agtagattta actttaccac tagttgtacg
     3001 cttagaaggt acaaatgttg agttaggtaa aaaaatctta aaagactcag gattagcaat
     3061 tgaaccagca gcaacaatgg ctgaaggtgc acaaaaaatt gttaaactag tcaaagaagc
     3121 ataaggaaag gatgggagca ctaagatgag tgtatttata gataagaata ctaaagtaat
     3181 ggtacaaggt attacagggt ctactgccct tttccataca aaacaaatgc ttgattatgg
     3241 tacgaaaata gtagcaggtg tgacgcctgg taaaggtggt caagttgttg aaggcgttcc
     3301 tgttttcaac actgttgaag aagctaaaaa tgaaactggg gcaacggttt cagtcattta
     3361 cgttccagca ctatttgctg cagactcaat tttagaagca gctgatgcag acttagatat
     3421 ggttatttgt atcactgaac atattcctgt attagacatg gttaaagtta aacgctactt
     3481 acaaggtaga aaaacacgtt tagttggtcc aaactgtcca ggtgtgatta cagcagatga
     3541 atgtaaaatt ggtattatgc ctggctatat tcacaaaaaa ggtcatgttg gtgtagtatc
     3601 acgttcaggt acactaacat atgaagcagt gcaccaattg actgaagaag gtattggtca
     3661 aactacagct gttggtattg gtggagaccc agtcaacgga acaaacttta ttgatgtttt
     3721 aaaagcattc aatgaagatg acgaaacgaa agcggttgtt atgattggtg aaatcggtgg
     3781 tacagctgaa gaagaagcag ctgaatggat taaagcgaat atgacaaaac cagttgtagg
     3841 ctttatcggt ggacaaacag cacctcctgg aaaacgtatg ggacatgctg gtgcgatcat
     3901 ttcaggtggt aaaggtactg ctgaagagaa aattaaaaca ttaaatattt gtggtgtgaa
     3961 aacagcggca acaccttcag aaattggttc aacattaatt gaagctgcta aagaagcagg
     4021 tatttatgaa tcattattaa cagttaataa ataaagttaa aagatgatat aaatggctat
     4081 agcccattcc atactttata aaagtatcgg aaatgggcta tttgtcgtgc gatgaatttt
     4141 agtacacttt tcatattttg gaaaggcaag ttaattattt tacctccttc aatagctttc
     4201 taaaacaaag caaactttta ttaaaatata tagtgcgcta tgttataata tttatatatt
     4261 attgtaagga gtgtttcatc atgaataaac aacaaagtaa agtacgctat tcaattagaa
     4321 aagttagtat tggaattttg tcaatttcaa taggtatgtt tttggcattg ggtatgtcga
     4381 acaaagcata tgcagatgaa attgataaat ctaaagattt tacaagaggg tatgagcaaa
     4441 atgtattcgc gaaatcagag ttaaatgcta ataaaaatac gacaaaagac aaaataaaaa
     4501 atgaagctgt tgttaaaaca tcggacacaa gtttaaagtt agacaacaaa tcagcaattt
     4561 caaatggaaa tgaaattaat caagatataa agatttcaaa tactccgaaa aactcaagcc
     4621 aaggtaacaa tctagttgtt aataacaatg aacctactaa agaaattaaa attgcaaact
     4681 tggaagctca aaattctaat cagaagaaag tgaataaagg tactaataat tactttggtt
     4741 actacagttt tagagaagct ccaaaaacac aaatctatac tgtaaaaaaa ggtgacacac
     4801 ttagtgctat agcattaaaa tacaaaacta cagtttcaaa tattcaaaat acaaataata
     4861 tagcaaatcc taatttaata tttattggtc aaaaattaaa agtgccaatg acaccattag
     4921 tagaaccaaa accaaaaaca gtgtcttcaa ataataaaag taatagtaat aacagtacat
     4981 taaattattt gaaaacatta gagaatagag gatgggattt cgacggtagt tatggatggc
     5041 aatgtttcga tttagtcaat gtatattgga atcatcttta tggtcatgga ttaaaaggat
     5101 atggagctaa agatatacca tatgcaaata attttaatag tgaagctaaa atttatcaca
     5161 acacaccaac tttcaaagct gaacctgggg acttagtggt ttttagtgga agatttggtg
     5221 gaggatatgg tcatacagct attgttttaa atggtgatta tgatggaaaa ttaatgaagt
     5281 tccaaagttt agatcaaaac tggaataatg gtggatggcg taaagcagag gttgcacata
     5341 aagttgttca taattatgaa aatgatatga tttttattag accatttaaa aaagcataat
     5401 ttaaatcgaa ggtaggacat tgaaatatga aattttcaac tttaagtgaa gaagaattta
     5461 ccaactacac caaaaagcac ttcaaacatt atacacagtc tatagaatta tataattata
     5521 gaaataaaat aaatcatgaa gcacatattg tgggagtgaa ggatgataaa aatgaagtta
     5581 tagctgcatg tttattaaca gaggcacgta tttttaaatt ctacaaatat ttctactctc
     5641 atagaggtcc tttacttgat tatttcgatg ctaaattagt ttgttacttt tttaaagaat
     5701 tatctaaatt catttataaa aatagaggag tatttattct tgttgaccca tatttaatag
     5761 agaatttaag agatgcaaac ggtaggataa taaagaatta taataattca gtgatagtaa
     5821 agatgctagg aaaaattggg tatctccatc aaggttatac aacaggatat tcaaataaaa
     5881 gccaaattag gtggatttct gtattggatt taaaagataa agatgagaat caacttttaa
     5941 aagaaatgga ataccaaact agaagaaata taaaaaagac tattgagatt ggtgttaagg
     6001 ttgaagattt atctattgaa gaaacaaatc gattttataa attgtttcaa atggctgaag
     6061 acaaacatgg ttttcatttc atgaatgaag attattttaa acgaatgcaa gaaatatata
     6121 aagataaggc aatgttaaag atagcttgta tagatcttaa tgaatatcaa gataaattaa
     6181 aaatacaatt attgaaaatc gaaaatgaaa tgatgactgt gaacagagca ttaaatgaaa
     6241 atccaaattc taaaaagaat aaatcaaaat taaatcagtt aaatatgcaa ttatctagta
     6301 ttaataatag aattagtaaa accgaagaac taatattaga agatggacct gttttggatt
     6361 tagctgctgc tttatttata tgtactgatg atgaagttta ttatctatca agtggatcaa
     6421 atcctaaata taatcagtat atgggagcat atcatctaca atggcatatg ataaaatatg
     6481 caaaatcaca taatattaat aggtataatt tttatggaat aacaggcgtc tttagtaatg
     6541 aggcggatga ttttggtgta caacaattta aaaaaggttt taatgcacat gttgaagaat
     6601 taattggtga tttcatcaaa ccagtaagac caattctata taaatttgca aaacttattt
     6661 ataaggtttg attataaagt atgttggaaa ttgaaattta aaattctttc caacatactt
     6721 ttcacttttt aagtcggtat tttactcctt aaaataacat tacatcgaca atgtttttgt
     6781 attagaaatg taaaacaaaa atagaatatg attttgtatg attaaaaaaa ggagttgatt
     6841 tattgattag actatttttg cttaagttat actgggcaca cttttcgact aaacaaattc
     6901 atcaattttt aatggcatat cctaatgtaa ttaaagaggg gggcagaaaa aaagatagtt
     6961 atttatgtga atgggtgaat agggaagaaa atgttcattt attacgtaaa tactatgctt
     7021 ttataaaact tgatcataac gacattatta aagaactgca gaaattaaaa gtaagttaca
     7081 ttacatatat ggatactgaa tacccagtgc tattaaaaga aatatatcaa tttccattac
     7141 ttcttttcta taaagggaac atcaaattaa taaataatat gcatcatttg gcagtagtag
     7201 gtgcaagaga ttctacgagt tatacccaac agtctttaga atttttatta tcaaatgata
     7261 aaagcaaata tttaacaatt gtttccggcc ttgctcaagg agctgatgca atggcacatc
     7321 aaatagcttt aaaatacaat ctccctacaa tagcagtttt agcctttggc catcaaacac
     7381 attatcccaa aagtacatta gcattaagaa ataaaataga agaaattggt ttagttatat
     7441 ccgaatatcc accacataca ccaattgcta aatatagatt tcctgagcgc aatagaatta
     7501 tcagcggttt gtcaaaaggg gttttaatta ctgaggctaa ggaacaaagt ggtagtcaca
     7561 tcacgataga ttttgcatta gagcaaaata gaaatgttta tgttttacct ggatctatgt
     7621 ttaatcctat gacaaaaggt aatttattac gtatccaaga aggtgctaag gtagtattaa
     7681 acgctaatga tatatttgaa gactactata tttaaaacta aaaataacga gttgcttcat
     7741 atttcttttg ttaccctttg taatttattt tttaataaaa tctataaaaa atagacaggg
     7801 aaaatgattt gtttagatat aaaacgttga caaaagcaaa attaagcgtt tatcatttat
     7861 ctttagtaat tagattagcg agggggaaat gacattggca gataatttag tcattgttga
     7921 atcgcctgca aaagcaaaaa ccattgaaaa gtatttaggt aagaaatata aagttatagc
     7981 ttcaatggga cacgtcagag acttaccaag aagtcaaatg ggtgtcgaca ctgaagataa
     8041 ttacgaacca aaatatataa caatacgcgg aaaaggtcct gttgtaaaag aattgaaaaa
     8101 acatgcaaaa aaagcgaaaa acgtctttct cgcaagtgac cccgaccgtg aaggtgaagc
     8161 aattgcttgg catttatcaa aaattttaga gcttgaagat tctaaagaaa atcgcgttgt
     8221 tttcaacgaa ataactaaag acgctgttaa agaaagtttt aaaaatccta gagaaattga
     8281 aatgaactta gtcgatgcac aacaagcgcg tcgaatatta gatagattag ttggctataa
     8341 catctcgcca gttctttgga aaaaagtaaa aaaagggttg tcagcgggtc gagttcaatc
     8401 tgttgcactt cgtttagtca ttgaccgtga aaatgaaatt cgaaacttta aaccagaaga
     8461 atattggact attgaaggag aatttagata caaaaaatca aaattcaatg ctaaattcct
     8521 tcattataaa aataaaccat ttaaactaaa aacgaaaaaa gatgttgaga aaattacagc
     8581 tgcattagat ggagatcaat tcgaaattac aaacgtgact aaaaaagaaa aaacgcgtaa
     8641 tccagcaaat ccatttacaa cttctacatt acaacaagag gcggcacgta aattaaactt
     8701 taaagctaga aaaacaatga tggtcgcaca acaattatat gaaggtatag atttgaaaaa
     8761 acaaggtacg attggtttaa taacatatat gagaactgat tctacacgta tttcagatac
     8821 tgccaaagct gaagcaaaac agtatataac tgataaatac ggtgaatctt acacttctaa
     8881 acgtaaagca tcagggaaac aaggtgacca agatgcccat gaggctatta gaccttcaag
     8941 tactatgcgt acgccagatg atatgaagtc atttttgacg aaagaccaat atcgattata
     9001 caaattaatt tgggaacgat ttgttgctag tcaaatggct ccagcaatac ttgatacagt
     9061 ctcattagat ataacacaag gtgacattaa atttagagcg aatggtcaaa caatcaagtt
     9121 taaaggattt atgacacttt atgttgaaac taaagatgat agtgatagcg aaaaggaaaa
     9181 taaactgcct aaattagagc aaggtgataa agtcacagca actcaaattg aaccagctca
     9241 acactataca caaccacctc caagatatac tgaggcgaga ttagtaaaaa cactagaaga
     9301 attgaaaatt gggcgaccat caacttatgc accgacaatt gatacgattc aaaagcgtaa
     9361 ctatgtcaaa ttagaaagta agcgttttgt tcctactgag ttgggagaaa tagttcatga
     9421 acaagtgaaa gaatacttcc cagagattat tgatgtggaa ttcacagtga atatggaaac
     9481 gttacttgat aagattgcag aaggcgacat tacatggaga aaagtaatcg acggtttctt
     9541 tagtagcttt aaacaagatg ttgaacgtgc tgaagaagag atggaaaaga ttgaaatcaa
     9601 agatgagcca gccggtgaag actgtgaagt ttgtggttct cctatggtta taaaaatggg
     9661 acgatatggt aagtttatgg cttgctcaaa cttcccggat tgtcgtaata caaaagcgat
     9721 agttaaatct attggtgtta aatgtccaaa atgtaatgat ggcgacgtcg tagaaagaaa
     9781 atctaaaaag aatcgtgtct tttatggatg ttcgaaatat cctgaatgcg actttatctc
     9841 ttgggataag ccgattggaa gagattgtcc aaaatgtaac caatatcttg ttgaaaataa
     9901 aaaaggcaag acaacacaag taatatgttc aaattgcgat tataaagagg cggcgcagaa
     9961 ataatatttt tatttcctag agacatttta agaatgttaa atagaatcat tagtgaatct
    10021 tattttaaag atagtaatgg attaatctat ataagtgcgg ataatataaa cataacaaca
    10081 taattaaaag acataaatga caataaaagg agtatagaaa tgactcaaac tgtaaatgta
    10141 ataggtgctg gtcttgccgg ttcagaagcg gcatatcaat tagctgaaag aggaattaaa
    10201 gttaatctaa tagagatgag acctgttaaa caaacaccag cgcaccatac tgataaattt
    10261 gcggaacttg tatgttccaa ttcattacgc ggaaatgctt taactaatgg tgtgggtgtt
    10321 ttaaaagaag aaatgagaag attgaattct ataattattg aagcggctga taaggcacga
    10381 gttccagctg gtggtgcatt agcagttgat agacacgatt tttcaggtta tataactgaa
    10441 gtacttaaaa atcatgaaaa tatcacagtt attaatgaag aaattaatgc cattccagat
    10501 ggatacacaa ttatcgcaac aggaccactt actacagaaa cccttgcaca agaaatagtg
    10561 gacattactg gtaaagatca actttatttc tatgatgcag ctgctccaat tattgaaaaa
    10621 gaatctattg atatggataa agtttactta aagtcccgtt atgataaagg tgaagctgca
    10681 tatttaaact gtcctatgac tgaggatgaa tttaatcgct tttatgatgc agtattagaa
    10741 gctgaagttg cgcctgtaaa ttcatttgaa aaagaaaaat atttcgaggg ttgtatgcct
    10801 tttgaagtaa tggcagaacg cggacgcaag acattactat ttggaccaat gaaaccagta
    10861 ggattagaag atccaaagac ggggaaacgt ccttatgcag tggttcaatt aagacaagat
    10921 gacgctgctg gcacactcta caatattgtt ggcttccaaa cgcatttaaa atggggagct
    10981 caaaaagaag ttattaaatt aattccaggt ttagaaaatg ttgatattgt tagatatggt
    11041 gtgatgcata gaaatacctt cattaactca ccggacgtat taaacgagaa atatgaattg
    11101 atttcacaac caaacataca gtttgcggga caaatgactg gtgttgaagg ttatgtagaa
    11161 agcgcagcta gcggcttagt tgcaggcatc aatcttgcgc ataaaatatt aggcaaaggt
    11221 gaggtagtat ttccgagaga aactatgatt ggaagtatgg cttactatat ttctcatgct
    11281 aaaaacaata agaatttcca acctatgaat gctaacttcg ggttattacc atctttagaa
    11341 actagaatta aagataaaaa agaacgctat gaagcacaag ctaatagagc tttggattac
    11401 ttagaaaatt tcaaaaaaac tttataaaat agttagaaag actagatatg ctattcattc
    11461 ttaagtcatc aacgagtaag taatgacttt ctaaatggaa aatacttatc ctagtctttt
    11521 taattttgga attgttacgt atttctgaca attgagaatt cgcattcaaa aaatatctaa
    11581 ataaataaca cgcaataagt tgattgatgt aacatgtaag agaatgtttt aaat
//
LOCUS       DAHHRR010000083        11617 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.083, whole genome shotgun sequence.
ACCESSION   DAHHRR010000083 DAHHRR010000000
VERSION     DAHHRR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11617
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.083"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..1329
                     /locus_tag="NBI42_001871"
     CDS             <1..1329
                     /locus_tag="NBI42_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778283.1"
                     /translation="NMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDILKEDMLNEYYYL
                     PKTLYNTLASPEFEDLKRTDASQVDGQDDTSEDDDNQSEKADSKSADSESKGGAYLEM
                     ELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNETLNREEGDAVGQSQAFQLDG
                     VNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIEREQRDARFNLTKG
                     RLQKDLINWFIDDQFKLFYKQQDLSKSFDATFTLLIDASASMHDKMAETMKGVVLFHE
                     TLKDLNIKHEILSFSEDAFDSDEHVQPNIINEIINYNYSTFEKDGPRIMALEPQDDNR
                     DGVAIRIASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEAVEMSRKFGIEVF
                     NVFLSQEPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLLLKSL"
     gene            1554..2897
                     /gene="brnQ"
                     /locus_tag="NBI42_001872"
     CDS             1554..2897
                     /gene="brnQ"
                     /locus_tag="NBI42_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD1778284.1"
                     /translation="MNKNTWIIGFTLFAMFFGAGNLIFPPNLGLDSGKFFWPAILAFV
                     LTGIGLPLLGVIVGSLDKEGYIGALNKISPKFSILFLVIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFLVVLYICINPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGFIGNHMPVSDATLQELNSKDRNIGTYLLT
                     TMAATGFGSLGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
     gene            complement(2970..4106)
                     /locus_tag="NBI42_001873"
     CDS             complement(2970..4106)
                     /locus_tag="NBI42_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCD1778285.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQTTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(4138..4767)
                     /locus_tag="NBI42_001874"
     CDS             complement(4138..4767)
                     /locus_tag="NBI42_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCD1778286.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(4786..5055)
                     /locus_tag="NBI42_001875"
     CDS             complement(4786..5055)
                     /locus_tag="NBI42_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCD1778287.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            5218..5526
                     /locus_tag="NBI42_001876"
     CDS             5218..5526
                     /locus_tag="NBI42_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCD1778288.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            5697..5897
                     /gene="cspA"
                     /locus_tag="NBI42_001877"
     CDS             5697..5897
                     /gene="cspA"
                     /locus_tag="NBI42_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HCD1778289.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
     gene            6094..6495
                     /locus_tag="NBI42_001878"
     CDS             6094..6495
                     /locus_tag="NBI42_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSA protein"
                     /protein_id="HCD1778290.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMMILVIPITITGI
                     LFFKTNLDKTYIFFNILFIDFYYYIYNVHLMALPRFNSYIKAEMMELEDIDVLITSKD
                     FGFDEILFFTLYLLLILIILYYLKKQVKTKS"
     gene            6629..7246
                     /locus_tag="NBI42_001879"
     CDS             6629..7246
                     /locus_tag="NBI42_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HCD1778291.1"
                     /translation="MHVPKYFQVDDFKEIEMFIQSNSFGMIVTVDQGKPLATHVPLVL
                     DKKGDDYYICGHLAKTNPQFNTLKDNQNVLIIYQGAHSYISSAWYEKENVPTWNYQAV
                     HLYGESSILSEEETIDDLKRLLVKYERHREDPVLWDNISENTKMQAKGIVGFKVKIHK
                     IEAAYKMSQNRNEKDYNNIIEKLEDESNASSVAVAEVMKEIHPYL"
     gene            complement(7325..8590)
                     /gene="lysA"
                     /locus_tag="NBI42_001880"
     CDS             complement(7325..8590)
                     /gene="lysA"
                     /locus_tag="NBI42_001880"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCD1778292.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKESGLKYNISYASKAFTCLQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENKIGYFVIDSLEEIDLIDRYANDTVQVVIRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKTAIEKVLQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LHWLKEHNIQVDLLNLGGGFGIKYVEGDESFPIEKGIKEITDAIKSEVQLLGIETPEL
                     GIEPGRSIVGEAGVTLYEVGTIKEIPGVNKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEDSDDTVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMSSNYNQMQK
                     PSVFFLKDGKAREVVKRQSLRQLIINDTK"
     gene            complement(8580..9665)
                     /locus_tag="NBI42_001881"
     CDS             complement(8580..9665)
                     /locus_tag="NBI42_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCD1778293.1"
                     /translation="MTATWSVNKKIFLQNAIAVKNNQPIMAVVKNNAYHYDLEFAVSQ
                     FIHAGIDTFSTTSLREAIKVRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHQNQNAKMIISGLWTHFGYADE
                     FDVPDYNVERSQWMEIVETLLFEGYQFDLIHAQNSASFYREGQELLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTVKANVIQVHEVQAGDYCGYSFAFEVTKTNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            complement(9670..10821)
                     /locus_tag="NBI42_001882"
     CDS             complement(9670..10821)
                     /locus_tag="NBI42_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1778294.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMQKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            complement(10964..>11617)
                     /gene="dapD"
                     /locus_tag="NBI42_001883"
     CDS             complement(10964..>11617)
                     /gene="dapD"
                     /locus_tag="NBI42_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCD1778295.1"
                     /translation="IKVYLNGNFEGITYPESFKVFGSEQSKVIFCEADDWKHFYEIYG
                     SQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIREQAIIEDGAVVMMGATINIGAV
                     VGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIEPPSASPVIIEDDVLIGANAVIL
                     EGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIKQASEVQDTKKEIVAALRKLND"
BASE COUNT         3768 a         1869 c         1850 g         4130 t
ORIGIN      
        1 aatatgttct tatatttacc taattttttc caaaatcaaa attctgaaga taatatgtat
       61 ctggctcaaa gaattatgta tcaagtagat gatattttaa aagaagatat gttgaacgaa
      121 tactattact tacctaaaac actatataat acattagcta gtccagagtt tgaagattta
      181 aagagaactg atgcaagtca agtcgatggg caagatgata cctcagaaga tgatgataat
      241 caaagtgaaa aagctgactc taaatctgca gatagtgagt caaaaggtgg cgcttattta
      301 gaaatggagc ttcacgaagg acaaaatagt gaaacattgg gtaatgatga agcacgtgaa
      361 ggcgatgcta cagatgatat gacagatatg atgaccaaaa agggcaaagg atcaaacgaa
      421 acgctaaatc gtgaagaagg cgatgctgtt ggtcaaagcc aagcatttca acttgatgga
      481 gttaacaaaa atgtagaaat aaaatggcaa attcctgaaa ttgagccaca atatgtttta
      541 gaatatcaag aatcgaaaca agacgtacaa tatgaaatca aagacttaat tcaaattata
      601 aagaaaacga ttgaacgaga acaacgagat gcacgtttta atttaacaaa agggcgatta
      661 caaaaggatt taattaattg gttcatcgac gatcaattta aattgttcta taaacaacaa
      721 gatttaagta aatcctttga tgcgacattc actttactta ttgatgcttc ggctagtatg
      781 catgacaaga tggctgaaac gatgaaaggt gttgtgttat ttcacgaaac actaaaagat
      841 ttaaatatta aacatgaaat tttatctttc agcgaagatg catttgattc agacgagcat
      901 gttcaaccaa acataattaa tgaaatcatt aattataatt attcaacctt tgaaaaagat
      961 gggccgcgta ttatggcatt agaacctcaa gatgacaacc gagacggtgt tgcgattcga
     1021 attgctagcg aaagattaat gcgtcgaaat caacatcaac gatttttaat cgtattttct
     1081 gacggcgaac cgtctgcatt taattatagt caagatggta ttattgatac gtatgaagct
     1141 gtagaaatgt cacgtaaatt tggcattgaa gtatttaatg tatttttgag tcaagaacca
     1201 ataactgaag atgttgaaca aacaattcat aatatttatg gtcaatatgc tattttcgtt
     1261 gaaggtgttg cgcatttacc tggacattta tcgccattac ttaaaaaatt acttcttaaa
     1321 tcattgtaaa tatacttaaa acctgagttt gtatgaaaag taaaataatt acctttaata
     1381 aatagcttaa atcttattgg aggtttctat tttttgaatc ataatctgat ttaaacatta
     1441 aattttttta tttctgatat gtacaacact tttcatatag actctttatt attttttgtg
     1501 ctaaaatatc tacttgtaaa ttatttcgtg ttttcaaaaa aaggagtaac tctatgaata
     1561 aaaatacatg gatcattggg tttacgcttt tcgctatgtt ttttggcgca ggcaatctga
     1621 ttttccctcc taatctcgga ctagatagtg gtaaattctt ttggcctgct attttagcat
     1681 ttgtcttaac cgggattggt ttaccattat taggagtgat tgtaggctca cttgataaag
     1741 aaggttatat tggagcgtta aataaaatat cgcctaaatt ttcaatattg ttcttagtta
     1801 ttatatattt gactataggt ccactttttg caataccgag aactgcatca acttcatttg
     1861 aaatgactat tacaccaatt atacatagta acagtagtat tgctttattt atttttacta
     1921 ttatctactt tttagtagta ttgtatattt gtataaatcc atcaaagtta atcgatcgta
     1981 ttggttcgct actaacacca ttattattga ttactatttt agcgatgatt attaagggat
     2041 acttagattt tagcggtaat agtgctggaa agggcaatga agcactatat cattctaatt
     2101 tttcaagttt tgctgaaggc tttacacaag gctatttaac aatggatgcc attgcagcaa
     2161 ttgctttttc aatgattgtt gttaatgcag taaaacttac aggtattact aaaacaaatc
     2221 aaatattcaa acaaactttg actgctggtt taatagcagc cgtggcattg attttcatct
     2281 atatttcatt aggttttata ggaaatcata tgcctgtgag tgatgcgaca ttacaggaat
     2341 taaattcgaa agatcgtaac attggaactt acttattaac gactatggcc gcaactggct
     2401 ttggatcctt aggtaaatat ttattaggta taattgtagc attagcatgt cttacgacag
     2461 catgtggtct tattgttgca gtatctgaat atttccatag aatcgtacct aaagtgtcat
     2521 acaaagcatt tgtattagtt ttcattttaa tgagttttat tattgctaac caaggtttaa
     2581 atgctgttat ctcaatgtca attccggtat taagcattgt atacccagta gcaataactg
     2641 ttgtgttatt aattttaatt gccaaattca taccgacaaa acgcatttca caacaaattc
     2701 cagttattat cgtatttata ttgtcgattt tcagtgttat tagtaagtta ggttggctga
     2761 aaattaactt tatagaatca ttgcctctaa gagcgtattc tttagagtgg ttcccagtag
     2821 caattattgc aacgatatta ggctatctag ttggcatatt tgtaaaacaa gatccaatta
     2881 aatatcaaca ggaataacga ataatataaa aggaagcatc tccaatttaa tcgtaggatg
     2941 cttccttttt gttatatcta attcgtaatt tatgattgtt tattcggtcc tttaatgttc
     3001 actaaatgtg actttaaatc ttgttctaat tgttgtaatt ctttttcagc taattgtcgt
     3061 tcttcgcgac cgtgttgttg aataattaat gtttcttcaa ttgtctcaat aatgttacgc
     3121 tgtgtacgtt tcaatgtatc aaggtcaaca atgccacgct cattttctgt tgcagtttca
     3181 atcgcatttt gtttcaacat ttcagcattt gctgttaata aatcattagt tgtatcagtg
     3241 acagctcgtt gtgcagcaac tgcattacgc tgtctcatta atgtaagcgc aatggccatt
     3301 tgatttttcc atagtggaat acttgtcaaa attgaacttt gtatcttctc ggcaagggct
     3361 tgattaacat tttgaatcat acgaatttgt ggcgcagttt gcaaagctat ttgtcttgaa
     3421 agctgtaagt catatatgcg tttatctagt ctatctataa attgctgcat atctgcaact
     3481 tgttgaatat ccatttgatt agtggtttgc tgcgcttgct gttgcaattg tggtagcttt
     3541 tcattttcta attgcaactt cttttgctgt gcagcaatga tatgcaatga taagtcatca
     3601 aagtattgtt tgtttttatc atatagcgta tctaataatt caatatctct tgttaaatgt
     3661 gtttgatgtt tctgcagttg aatcgtgatg cgatcgactt gagcactaac tgattgcatt
     3721 cttgaaaaga tttcattgat agacgacttt gctctgctaa aaattctttt taacatagat
     3781 ggtttatcag tatttaactc atttggatta actgacttta gttttgacat gagatctgac
     3841 aaagtatcac caataggacc aacatcttta ctttgtactt catccaacat ttgatgtgaa
     3901 aattgagaca tttgtttctg taaatcagaa ccaaacgcta ataaaccttc attgtctaaa
     3961 gggttaattt gtttactgat tgtgtctacc tgtttttgtt gttcaattgt taacttttct
     4021 ttttgaatag tagtattcga taattcttta tcgcttataa aatcatctag tggatggctt
     4081 tctttgaaac ttttattttc agtcatcaat catctctcca atagttgcta ttttacatca
     4141 atcttgatga tgcttttgat gtaacttatt taattccatt tcaatatcta aacgttcata
     4201 atcatcttcg ttgagacgct ttaaatcagc gattaatgtt cgtttgacct catccaaagt
     4261 aattcgtgtt tgttctaatt tttgctgttc attaattgat ttttttggca tttttgctag
     4321 acgtgtatat gcatcaacca aatttaaagc attatcaata tgagaataaa aaaagccttc
     4381 aactttataa aatgatgcag gtctctgtct aactgtcgta taaatagaac gtgaaatttg
     4441 gtatatatca ttaatttgcc taaaatcttt aattgatctt atattgacat acgtttttaa
     4501 aatacctcta agtttttggt gtgtatgatt taactgattt cgaatatagc gatagtcttt
     4561 tctagtcaaa ccaatttcgt ttaaatattt gcgtgaagtg agtttttgta tcggtaggta
     4621 tgtcattaaa aagccaacaa taccaataga catatcaatt aaaaaagata catcaagtgc
     4681 aatcatccca aatatgcttg ttaaaaacgc tacaggaatt cccactaaca ccccaaatat
     4741 atgagaaata ttatatctca ctgtcatctt cctttattta gcattttata ttgatcgaaa
     4801 atccgataat ttttgattta gttctaactc ttcaagttga tggctcgtta catttgatgc
     4861 tggtgaggca ccttcaatta caccttgaat aaatctctct atatctgcgt catccccttg
     4921 tgcatatatc tctacatagt catctacatt ttgaacagta ccgacaatgt tatagttcat
     4981 tgcaatgcgt tgtgtaaaat atctaaaacc gacgccttga acgcgtccga atacttgtaa
     5041 atgtatatgt ctcattttta ccacctcata gtattattat acgtagtttt acttaaaaaa
     5101 actaataatt actatagtta atactttgtt tgtttcaagt cgtcaaactt gattttcaga
     5161 ggataaaggt ataaaaataa gtatagagtt tttgaagtat ggaaggggtc tttaataatg
     5221 tggacagtta ccaaaattag agccgattat gagggatggt ggttattcag tgactggcca
     5281 gaaaacattg ttgaaaaata tcaatatcaa gattttgatg acatgtttaa gcactatcaa
     5341 caattgatta atcaatgtaa agttcagttc gataattatg tcacaggcaa atataatatt
     5401 tatgcatttt ataataattg tgacatgaac tattgcgaag attgtgaaga ggatttacaa
     5461 atattttata gtttcattgt tttacaaaat aatgaagtat attataaact accaataatt
     5521 gattaaatga atatttttta ttattgtatt gcaatttggt aataactgca ctataataca
     5581 agttaagcag atgattattc catattgcaa agaatatttg agtgaaattt gcatgtattt
     5641 tttgtaatat gcgaataagc atattgaatg aattttagtc ttggaggttt cagattatga
     5701 aacaaggtac agttaaatgg tttaacgctg aaaaaggatt cggctttatc gaagttgaag
     5761 gagaaaatga cgtattcgta catttttcag caattaacca agatggttac aaatcattag
     5821 aagaaggtca agctgttgag tttgaagtag ttgaaggcga ccgcggtcca caagctgcaa
     5881 acgttgttaa actataattc ttagatttga atcattgatt ttaactaaca ccttaccaag
     5941 aagtaaggtg tttttatatg cttattacaa atacttaacg cgatgagttt ctcaaccaaa
     6001 ttgagaactc tttttttata tcaccaacat tgtttgcaat agaaaattat atttatataa
     6061 tataattatg gtgtacaatg acgaggtgat tttatgaaat atcttatcct tagtttagtt
     6121 gctaatttat tagttttcgg tgtattatct gcaattggtt taaatattaa tattttagca
     6181 gcaatgatga tgattctagt tattccaatt acgataacag gaatattatt ctttaaaact
     6241 aatttagata agacatacat attttttaac atacttttta tagactttta ttactatatt
     6301 tacaatgtac atttaatggc cttaccaaga tttaacagtt atattaaagc agagatgatg
     6361 gaattagaag atattgatgt attaattaca agtaaagatt ttggatttga cgaaatttta
     6421 ttttttacac tgtatttgtt attaatcctt atcattttat attatttaaa aaagcaagtt
     6481 aaaactaaaa gttaaagcct aattattttt gtttcatagt ttattttata aacgagtgat
     6541 actatggcat tactctaact tatttatcct tagatttttt aatgataaaa tatattaaaa
     6601 tgaattgtta ttcacagggg gagatcatat gcatgtgcca aagtattttc aagtagatga
     6661 ttttaaagaa atcgaaatgt ttattcaaag taattctttt ggaatgattg taactgtgga
     6721 tcaaggaaag cccttagcta cccatgtacc attagtttta gataaaaaag gggacgacta
     6781 ttatatttgt gggcatctag ctaaaactaa tcctcaattt aatacattaa aagataatca
     6841 aaatgtatta atcatatatc aaggtgcaca ttcttacatt tcttcagcgt ggtatgaaaa
     6901 agaaaatgta cctacatgga attatcaagc agttcattta tatggtgaat caagtatatt
     6961 aagtgaggaa gaaacaattg atgacttgaa aagactattg gtaaagtatg aaaggcatcg
     7021 tgaagaccca gtattatggg acaatatttc tgaaaatacc aaaatgcagg ccaaaggtat
     7081 tgtaggcttt aaagtcaaga tacacaagat agaggctgca tacaaaatga gtcagaatcg
     7141 taatgaaaag gattataata atataattga aaagcttgaa gatgaatcta atgcaagcag
     7201 tgttgctgta gctgaagtaa tgaaagaaat tcatccttat ttataattta acaatcattt
     7261 aataataact aaaaaaatcc ccaagtcaca gttataactg atgttagggg atatatttta
     7321 tagattattt cgtatcatta ataataagtt gtcttaatga ctgacgtttt acgacttcac
     7381 gtgccttacc gtcttttaag aaaaatacgg atggcttctg catttgattg taatttgatg
     7441 acatagagta atggtatgca cctgttgaaa ggattgcaag gtaatcacca cgttttactg
     7501 atgacggtaa cttagcatct ttaataatga tatctccaga ctcacataat tttcctgcaa
     7561 tagtaaccgt atcatctgaa tcttcatttc tgttaactaa taatgcctga tacttcgcat
     7621 cataaagtgc agttctgata tggtcactca taccaccatc aattgaaacg tatttattaa
     7681 ctccaggaat ttcttttatt gttccgactt catataaagt gacaccggct tcaccaacaa
     7741 ttgaacgacc tggttcaata cctaattctg gggtttcgat accaagaagc tgtacttcag
     7801 attttatcgc atctgtaatt tccttaatac ctttttcgat agggaaactt tcatcacctt
     7861 cgacatattt aataccaaag ccgccaccaa gattcaataa atcgacttga atgttgtgtt
     7921 cttttaacca atgcagtacg attttagcag tttcgataaa tgcctctgtt ccttcaattt
     7981 gagaaccaat atgacaatga acacctttta attttaaatg tttggattgt aggacttttt
     8041 caattgctgt tttggctaag ccatattgaa ttgataatcc aaatttacta tcttcttgtc
     8101 cggtttgtat aaattcatgt gtatgtgctt caacccctgg gttcacacgt ataactactt
     8161 gaaccgtgtc attggcatat cgatcaatta aatcaatttc ttctaatgaa tcaatcacaa
     8221 aatatccgat tttattttct aaagcatatc taatttcatg tttcgtttta ttgttaccgt
     8281 gaaaatgaat acgacttggt tcaaagcctg cttctaatgc cgtgtataat tcaccttcag
     8341 aaacaacatc taattgtaaa tcttcttcag ctacaagttt gaccatttga aggcaagtaa
     8401 atgcttttga agcatatgag atattatatt ttaagccact ttctttaaat gcgcgatgat
     8461 aacgacgcat ttgttctcta atttgtaatt cgtcatatac aatggtaggg gtaccgaagc
     8521 tttgcgcaat cgtttttaaa ctaataccat ccattgttaa ttcgccattt tgattatatt
     8581 taacagtcat ttgaaatgta ctcctttttt aaagaatcat gattcatagc acttaattgt
     8641 tcagaatttg caccaacacc tttaaaggta tattcatcga tgcggatatc attattataa
     8701 agaataactt catcttgtgc atgtacattg ccatctactt caacaaacat atggctcatc
     8761 attaatgcac gtatcgggta gcgtttacca ttgataagtg cttcatgttt agcacgcgtt
     8821 cttaaaattc catcgccata accgatatca actacagcta attttgtatt ggttttagtc
     8881 acttcaaagg caaagctata accgcaataa tcaccagctt gtacttcgtg cacttgaata
     8941 acatttgctt ttacagtcaa tgactgaaca atatcatgtt gattcagtga actatatggt
     9001 cttgaaccgt atagtgcaat acctacgcgt gcgtgtgtat ggtgggggag taattcttgt
     9061 ccttcacgat aaaaacttgc gctattttgt gcatgtatta gatcaaattg ataaccttca
     9121 aataaaagtg tctcaacaat ttccatccat tgtgaacgtt caacattata atctggcaca
     9181 tcaaactcat cagcgtatcc aaaatgagtc cacaaaccac ttattatcat ttttgcattt
     9241 tgattttgat ggtgatcttt caatacttct ttaatttcgt ttaaatcttt aaatccagac
     9301 cgatgtaata aattttcaaa ttctaagtga acatgaatac cagctaaatc atttttatgg
     9361 ttatagtaat atgtcaacga cggcaaagtc atgtgtattt gatgttcacg gactaaatca
     9421 aactcgtaaa ctgcattcat taaaaagatt gttgcatctg gagcaagttg tctaacttta
     9481 atcgcttcac gtaatgacgt agtactaaac gtatcaatac cagcatgaat aaattgggat
     9541 acagcaaatt ctaggtcata gtgatatgca ttatttttaa caactgccat tattggttga
     9601 ttgtttttaa ctgctattgc attttgtaaa aatattttct tatttacaga ccatgttgct
     9661 gtcaatgtat tacacctctt tgtaattatt taataaattt tcgtaaaaat taaccacgtt
     9721 tattaacact ttttcatcaa aatttaaatg tgatgtgtgc aaaccagtta caaaaccttt
     9781 atcttcattt cgtgttccta taaaaacaaa gtaagctgga gctagttgtt gaccataaaa
     9841 actaaaatct tccccaaata agaatggcgt tggtttgtca tagacattta aatcagcttt
     9901 tattaaagcg tcctctattt gagtacgtaa tttcggacta ttgattgtag ggggataacc
     9961 ttctgcaaat ttaacttcac agtctacatt aaacagaagc ttgacacttt ctgctatctt
    10021 ttgcatttga tttttaacga ttgttaaatc atcaatatca tatgtacgaa ttgtaccttc
    10081 taaataacca ttacttggta cagtattaat cgcttcacca gctttaaaat gaccaatatg
    10141 aacaatattt cgtttcaaac cgttaaggtg aaattgttga atttgtgata tttgagttaa
    10201 tacatgttgt aatgcttcac cacaagaatg accttgttct ttatcagcaa catgacttga
    10261 caggcctgtt aagaaaaagc gatactctgt tgcactggcc gtaatttctt catctcttat
    10321 cactgcaatg ccttcatcag caaatgggtt aacatgaata ccaaataccg cttcaattgg
    10381 atacttatca aaggcaccgg cttttattaa tcgatttgca ccgccaccag tttcttctgc
    10441 aggttggaaa atgaaaacga cattttgcgg taattgaccc gcatcttgca tgtctttgca
    10501 acgttgtaca aaaagcatta atgcagttgt atgaccatca tgtccacaag catgcatcac
    10561 atgatcagat tgactgcgat aaggcacatc attttcctct aaaataggta acgcatcaat
    10621 atcagctcta tacgctatcg tatgtgagcc attaccttct aagtatgcaa tgacgccagt
    10681 ttccaatggg caatcgtatt taatatttaa actatctaaa aacgctttaa tataagcagt
    10741 tgtttcaaat tcatgtaaac ttaattcagg atgttgatgt aaatgacggc gatgtttcgt
    10801 aacaaattct aattcattca taattatcaa tcctttgtgt taaattacta tataaatagt
    10861 gtcacgtatt tcgaaatttg tgatcataag tttattcaat gataaacaat aaggttgaga
    10921 cataatcgta tctcaacctt gaaattatta tacgttgacg tcactagtca ttcagttttc
    10981 ttaatgctgc tacaatctct tttttagtat cttgtacttc agaagcttgc ttaatcactt
    11041 tcgcaggtgt accagcaaca actgcaccag ctggtacatc ttgtgtcaca atcgcgccag
    11101 ctgcaacaat agcaccttta ccaacacgta caccttctaa aataactgca tttgcaccga
    11161 ttaatacatc atcctcgatt ataactggtg aagcgctagg gggttcaatc acacctgcta
    11221 atactgcgcc agcccctaca tgtacatttt tacctgttgt agcacgacca ccgagagtag
    11281 cattcatatc aatcatcgta ccttcgccaa cgactgcgcc aatattaatt gttgcgccca
    11341 tcataacgac agcaccatct tcaataatgg cttgttctct aataaacgca cctggttcaa
    11401 ttcgtgcatt cgtatttgtt aagtctttta atggaatggc agaattgcga cgatccattt
    11461 caatttctat atcttcgaat tgactaccat atatttcgta aaaatgtttc caatcatctg
    11521 cttcacaaaa gattacttta gattgttctg aaccaaatac tttaaaactt tctggatatg
    11581 taatgccttc aaaattacca tttaaatata cttttat
//
LOCUS       DAHHRR010000084        11458 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.084, whole genome shotgun sequence.
ACCESSION   DAHHRR010000084 DAHHRR010000000
VERSION     DAHHRR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.084"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            19..708
                     /locus_tag="NBI42_001884"
     CDS             19..708
                     /locus_tag="NBI42_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1778296.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(1213..1626)
                     /locus_tag="NBI42_001885"
     CDS             complement(1213..1626)
                     /locus_tag="NBI42_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD1778297.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            1831..3129
                     /locus_tag="NBI42_001886"
     CDS             1831..3129
                     /locus_tag="NBI42_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCD1778298.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            3582..4523
                     /gene="pde2"
                     /locus_tag="NBI42_001887"
     CDS             3582..4523
                     /gene="pde2"
                     /locus_tag="NBI42_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HCD1778299.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGSKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLITHFNDEAIVNKDIARVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITKDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGSEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVNSWDEFEQLATALRTKLN"
     gene            4544..7741
                     /locus_tag="NBI42_001888"
     CDS             4544..7741
                     /locus_tag="NBI42_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCD1778300.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVEALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTIETVVLAKDNNGLKDLYQLSSKIKMNSM
                     ENVSFELLQQFSSNLIIIFKNVADEHRDIVQVFDSHEDTYLDHRSVLVQGIKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEIKQLDINQGYLTQVDV
                     IAQKCNAELKYHQSLLPQYQTPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLRITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFDLLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKENGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QIYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNNKPILVQ
                     FDKVKQIRTKNGKNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDLR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSHIDTFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
     gene            8191..9420
                     /locus_tag="NBI42_001889"
     CDS             8191..9420
                     /locus_tag="NBI42_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCD1778301.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            9615..10472
                     /locus_tag="NBI42_001890"
     CDS             9615..10472
                     /locus_tag="NBI42_001890"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCD1778302.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            10472..11416
                     /locus_tag="NBI42_001891"
     CDS             10472..11416
                     /locus_tag="NBI42_001891"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCD1778303.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVEQLDSLESLSRDEIANDRFEKFRNIGSYIE"
BASE COUNT         3976 a         1598 c         2173 g         3711 t
ORIGIN      
        1 tgataggagg atgctcaaat gaagttatca tttcatggtc aatcaacgat ttatttagaa
       61 gggaacaaca aaaaggttat cgtagatccg tttatatcaa ataaccctaa gtgtgatttg
      121 aatattgaaa cagtgcaggt agactatatc gttttaacgc acggtcattt tgatcacttt
      181 ggtgacgttg tggaactagc aaagaaaaca ggagcaacag taattggaag tgcagaaatg
      241 gctgactatc tttcttcata tcacggtgtt gaaaatgttc atggtatgaa tattggtggt
      301 aaagcaaact ttgatttcgg aagcgttaaa tttgttcaag catttcatag ttcaagtttt
      361 acacatgaaa atggtattcc agtttattta ggtatgccaa tgggtatcgt gtttgaagtt
      421 gaaggaaaaa caatttatca tactggtgat acaggtctgt ttagtgacat gtcacttatt
      481 gcgaaacgtc atccagttga tgtatgtttt gtaccaattg gagataattt tacgatgggt
      541 attgatgatg caagttatgc tattaatgaa tttattaaac caaaaatttc tgtaccgatt
      601 cattatgata cattcccatt aatcgaacaa gatccacaac aatttaaaga tgctgttaat
      661 gtaggggacg tgcaaatttt aaaaccaggt gaatctgttc agttttaaat agaatataga
      721 ttttacacta aaaaacacct cgctaatcta tgtggaagtt atcgtccgca tatttttagt
      781 gagtgttttt atattctact gatattgtaa gctgactttt ccgtcagctt acatgttggg
      841 gccccacccc aacttgcaca ttattgtaag ctgacttttt cgtcagcttc catgttgggg
      901 cccagcccaa cttgcattgt ctgtagaatt tcttttcgaa attctctatg ttggggccca
      961 ccccaacttg cacattatag taagctgact ttttcgtcag cttccatgtt ggggccccat
     1021 agtcaactcc tgctgggtta tgatattgat tatgtttttg gttcaaaatg gttttgtttg
     1081 atacaagttg ttggttagat agtgaataat ataaaaaatc cagaaccaca gtcataatca
     1141 tttcttgtgt ttactcagtc gtttgagact gcatcatgat tgaaaggtta tttgtgattc
     1201 tggataaaaa ttttatttaa cgattaatac agggatagtc gcacgttttg ctactttgtg
     1261 gctgacactt ccaagtacaa atttcttttt gtcttctgct ttacggttac ttaaaacaac
     1321 aatttcgtat ttaccgctat tagcatgttt taatagttct tcttttgcat taccacgaac
     1381 aattatttgg tcataatcaa taccataatc ttctaaagct ttgcgtgttt tttccaatcg
     1441 cttgcttcgt tcttcagtaa gtttgtttaa atgaacacct gctttaattg atgcttgagc
     1501 atcttgttcg ctgattgcgt ttaaaactgt tacgactgta ccttcgccag ctaatttaga
     1561 cacttctttt agtgcttttt catttttcaa ctgagtgtct acaccaagta atatattttt
     1621 atacattagt acatccctcc tcacttacat tctaataaat aataatggat ttttccattt
     1681 aaaattaaag attttgtgat ctttgtgttt gttgtatatt ttcaaaaaat aaaataggtg
     1741 aaacaatcat tatttcattt cacttcaatg ctttttatcc aaaataaagt tataatgtag
     1801 ttaatactgt tcgaaaagaa gaggtaaaga atgacaaaac atgaacaaat tttagattat
     1861 attgaatcgt tatctatagg ctctaaaatt tcggtgcgta aaatcgctaa atttttaaac
     1921 gtatctgaag ggacagcata tcgtgcaatt aaagatgctg ataaaatggg tatggtcgca
     1981 acgatagatc gtgttggtac ggttcgaatt gaaaaacgta atcgtaatga aattgaacat
     2041 ttaactttta atgaaattgt caatattatt gatggccaag tgttaggtgg caacaaaggt
     2101 atcactaaga tggtatctaa atttgcaatt ggagcaatgg aactcaaaga tattttaaag
     2161 tatattggtc caaagacatt attgattgtc ggaaatcgcg aagatgtgca gattgaagct
     2221 ttaaaaagag gcacggctat attaattact ggtggattta agccatctaa taaagttatt
     2281 aattttgcga atgaacatga tttacctgtt ctatcatcaa gttatgatac attcctagtt
     2341 gcaaatatta tcaacaaagc actttttaat cagaaaatac gaaaagatat cttaatagtt
     2401 caagatataa tgacgccatt agatgattta tctgtactgt ttgatacgat gaaaatagca
     2461 gattataagc gtatggcgaa tcgaactggc catacaagat ttccggtagt caatgagtct
     2521 tataaattag taggtattgt aacaagtcga gagatgatta atacaaagga cgacgacgaa
     2581 atagataaag taatgacgcg aaatcctatt tatgtaaatg ctatgagtac agtagcaagt
     2641 tgtgcgcata tgatgatttg ggaaggtatt gaattgattc ctgtcgtttc gagtaataaa
     2701 aagacagtgg gtgtgattaa tagacaagat gttttaaagt ctatgcaact tttaggaaga
     2761 cagcctcaaa tgggtgaaac aattaacgat caaatcgcca aatatataac aatgaatcaa
     2821 gatggaatta cagtagaagt ttcaccactg ttaatcaatc actacggaac agtaagtaaa
     2881 gcggcatttg taagtattat agaagaaaca atacaatatg aaatgcgtaa atttaaaaaa
     2941 ggtaatgtca tgatagagaa tttaaatatc gtgtatatta aaacagtacc tattgaatca
     3001 catataacag tgcgcttcgg catactagac gtcggaagaa acttcgccaa aatagaggtg
     3061 aacatgcata gtcaaaacga caaagtagcg agcgctctcg tgatatgcca aatgtttgac
     3121 gaagtatagg aacttagcag agaaaatagg cataagatag atatcgcaca aatgttggag
     3181 cctaacttgc acattatagt aagctgacat tacgtcacct tctatgttgg gccccacccc
     3241 aacttgcatt gtttgtagaa tttcttttcg aaattctttg tgttgggtcc ccaccccaac
     3301 tggcattgtc tgtagaaatt ggggacccaa tttctctgtg ttggggcccc ggaattaaaa
     3361 agcttgttat atgtgtattt tttatttggt caactgttgc taatataata ttgattgatg
     3421 tcctatactc gttatgaact aaatagtatg gaaatatata tatctaaata attaaaacgg
     3481 gcaaatagtt taatatggta gtaacaattt ttataattta gatattttga tatagactaa
     3541 tgcttaaatt aagcaacttt tctaaaaaag ggagtatgaa aatgattagt actttgaatg
     3601 aaattatgaa atgtatcgaa gacaacgata caattattat acacagacat gttagaccag
     3661 atcctgatgc atatggttca caacttggtt taaaatacta tattcagcag aaatttccgc
     3721 aaaagcaagt atttgcagtg ggtgaagcgg aatcatcatt aagttttatt ggagagttag
     3781 ataacattga tgataaaaca tatcaagatg cgcttgtaat tgtatgtgat actgccaatg
     3841 ctccacgaat tgatgatgaa cgttatagta caggtagtaa acttattaaa atagatcatc
     3901 atcctgcagt tgatcagtat ggtgatatta atttagttaa tacgaacgcg tcatctacaa
     3961 gtgaaatcat ttatgattta atcacacatt ttaatgatga agcaattgtt aataaagata
     4021 tcgcgagggt tttatacctt ggtatcgtcg gtgatactgg gcgattcctt tttaacaata
     4081 cctcagaaca tactatggaa attgctggaa agttaattgg gcatgatatt gatcataatg
     4141 cgcttttaaa taaaatgatg gagaaggatc caaaaatgtt gccgtttcaa ggttatgttt
     4201 tacaacattt cgaacttatg gatgatggat tctgccaagt taaaataact aaagatgtat
     4261 tggagcaatt cggtattcag ccaaatgaag catctcagtt tgttaataca attgctgaca
     4321 ttaaaggttt gaaaatatgg gtgtttgcag ttgatgaggg tagtgaaata agatgtcgct
     4381 tacgctctaa agggcaattg attattaatg atattgcgca agattttggt ggcggtggtc
     4441 atccgaatgc gtcaggagtt tcagtgaaca gctgggatga atttgagcaa cttgctacag
     4501 ctttacgcac aaaacttaac taatagaaag gagccattca atcatggtgg catatttaaa
     4561 tattcatacg gcttatgatt tgttaaattc aagcttaaaa atagaagatg ccgtaagact
     4621 tgctgtgtct gaaaatgttg aagcacttgc cataactgac accaatgtat tgtatggttt
     4681 tcctaaattt tatgatgcat gtatagcaaa taacattaaa ccgatttttg gtatgacaat
     4741 atatgtgaca aacggtttaa acactattga aaccgttgtt ttagcaaaag ataataatgg
     4801 gttgaaggat ttatatcaac tttcatcgaa aataaaaatg aattcaatgg aaaatgtttc
     4861 atttgaacta ttgcaacaat tttcatcgaa tttgattatc atttttaaaa atgttgcaga
     4921 cgaacatcgt gacattgttc aagtttttga ttcgcatgaa gatacgtatt tagatcatcg
     4981 aagtgtttta gttcagggta taaagcacgt atggattcaa aatgtttgtt accaaacacg
     5041 tcaagatgcc gatacgattt ctgcattagc agctattaga gacaatacaa aattagactt
     5101 aattcatgat caagaagatt ttggtgcaca ttttttaact gaaaaggaaa ttaagcaatt
     5161 agatattaat caaggatatt taacgcaggt tgatgttata gctcaaaagt gtaatgcaga
     5221 attaaaatat catcaatctc tacttcctca atatcagaca cctaatgatg aatcagctaa
     5281 aaaatatttg tggcgtgtct tagttacaca attaaaaaaa ttagaactta attatgacgt
     5341 ctatttagag cgattgaaat atgagtataa agttattact aatatgggtt ttgaagatta
     5401 tttcttaata gtaagtgatt taatccatta tgcgaaaacg aatgatgtga tggtaggacc
     5461 aggtcgtggt tcttcagctg gctcattggt cagttattta ctaggtatta caacgattga
     5521 tcctataaaa ttcaatctat tatttgaacg ttttttaaac ccagaacgtg taacaatgcc
     5581 tgatatagat attgactttg aagatacacg ccgagaaagg gttattcagt acgtccaaga
     5641 aaaatatggc gagctacatg tatctggaat tgtgactttc ggtcatctgc ttgcaagagc
     5701 ggttgctaga gatgttggtc gaattatggg gtttgatgaa gttacattaa atgaaatttc
     5761 aagtttaatc ccacataaat taagaattac acttgatgaa gcatatcaaa ttgacgattt
     5821 taaaaaattt gtacatcgaa accatcgaca tgaacgttgg ttcagtattt gtaaaaagtt
     5881 agaaggttta ccaaggcata catctacaca tgcggcagga attataatta atgaccatcc
     5941 attatatgaa tatgcccctt taacgaaagg ggatacagga ttattaacgc aatggacaat
     6001 gactgaagcc gaacgtattg gtttattaaa aatagatttt ctagggttga gaaacttatc
     6061 gattattcat caaatcttga cacaagtcaa aaaagattta ggtattaata ttgatatcga
     6121 aaaaattccg tttgatgatc aaaaagtgtt tgatttgttg tcgcaaggag atacgactgg
     6181 catattccaa ttagagtctg acggtgtaag aagtgtatta aaaaaattaa agccggaaca
     6241 ctttgaagat attgttgctg taacttcttt gtatagacca ggtccaatgg aagaaattcc
     6301 aacttacatt acaagaagac atgatccaag caaagttcaa tatttacatc cacatttaga
     6361 acctatatta aaaaatactt acggtgttat tatttatcaa gagcaaatta tgcaaatagc
     6421 gagcacattt gcaaacttca gttatggtga agcggatatt ttaagaagag caatgagtaa
     6481 aaaaaataga gctgttcttg aaagtgagcg tcaacatttt atagaaggtg caaaggaaaa
     6541 tggttatcac gaagacatta gtaagcaaat atttgatttg attctgaaat ttgctgatta
     6601 tggttttcct agagcacatg ctgtcagcta ttctaaaatt gcatacatta tgagcttttt
     6661 aaaagtccat tatccaaatt atttttacgc aaatatttta agtaatgtta ttggaagtga
     6721 gaagaaaact gctcaaatga tagaagaagc aaaaaaacaa ggtatcacta tattgccacc
     6781 gaacattaac gaaagtcatt ggttttataa accttcccaa gaaggcattt atttatcaat
     6841 tggtacaatc aaaggtgtag gttatcaaag tgtgaaagtg attgttgatg aacgttatca
     6901 gaacggcaaa tttaaagatt tctttgattt tgctagacgt ataccgaaga gagtcaaaac
     6961 gagaaagtta cttgaagcac tgattttagt gggagcgttt gatgcttttg gtaaaacacg
     7021 ttcaacgttg ttacaagcta ttgatcaagt gttggatggt gatttaaaca ttgaacaaga
     7081 tggtttttta tttgatattt taacgccaaa acagatttat gaagataaag aagaattgcc
     7141 tgatgcactt attagtcagt atgaaaaaga atatttagga ttttatgttt cgcaacaccc
     7201 agtagataaa aagtttgttg ccaaacaata tttaacaata tttaaattga gtaacgcgca
     7261 gaataataag cctatattag ttcagtttga taaagttaaa caaattcgaa ctaaaaatgg
     7321 taaaaatatg gcattcgtca cattaaatga tggcatcgaa actttagatg gtgtgatttt
     7381 ccctaatcag tttaaaaagt acgaagagtt gttatcacat aatgacttgt ttatagttag
     7441 cgggaaattt gaccttagaa agcaacaacg tcaactaatt ataaatgaga ttcagacatt
     7501 agccactttt gaagaacaaa aattagcatt tgccaaacaa attataatta gaaataaatc
     7561 acatatagat acgtttgaag agatgattaa agctacgaaa gaaaatgcca atgatgttgt
     7621 gttatccttt tatgatgaaa cgattaaaca aatgactact ttaggctata tcaatcaaaa
     7681 agatagtatg tttaataatt ttatacaatc ctttaaccct agtgatatta ggcttatata
     7741 acttttatca actaataaat tatgatatag taaactgatg gttagatatt ttttaaccat
     7801 cagtttgctt ttatattagt tttttatgct tattattttt atgagtttca ctttacatca
     7861 ttgattaatc cataagaata attagcacaa aaagcagtgt acataaattg agtaaagaat
     7921 ttgtcgatat atcgtgctaa aaaataataa ctttttgata tattggtttt tgctatccat
     7981 tataataatt aaaggtatat ctttagatag tatggtttaa tgtatcttta aaaatattat
     8041 tttattatcg tttatcgcta tttgtgttac ataatcaaaa ccataaattc ttactcattc
     8101 agatttaccc aatattttta cttttataat gtaatgcgtt ttatccaagt tattttttaa
     8161 aaaataaata ttgaattagg ggctgatttc atgtcattaa gagatgaagc attggaaatg
     8221 cacaaacgta atcaaggtaa attagaagtt aaaccaaatg taaaagttac taataaagag
     8281 gaattaagtt tagcatactc acctggcgtt gctgaaccgt gtaaagatat atatgaagat
     8341 aaaagaaaag tgtatgatta cacaattaaa ggaaatacag ttgcagttat tactgatgga
     8401 acagcggtat taggtttagg gaacattgga cctgaagcaa gtattcctgt aatggaaggt
     8461 aaagcagtat tattcaaaag cttcgctggt atcaatgggg ttcctattgc gttaaataca
     8521 actgataccg aagaaatcat taaaacagtt aagttgttag aacctaatta tggtggtatt
     8581 aatttagagg atatttcggc accacgttgt tttgaaattg aagaacgatt gaaaaaagaa
     8641 actaacattc cggtattcca tgacgatcaa catggtacag cgattgtaac aatggcaggt
     8701 ttggtaaatg cattgagagt tgttaataaa gatattgcta aaataaaagt tgtactaaat
     8761 ggtgctggtg cagcaggaat agccattgtt aaattactat acgcgtatgg tgtaagaaat
     8821 atggttatgt gtgactcaag aggcgcaatt tttgaaggac gttcatatgg tatgaatcct
     8881 acgaaagatg ttgtagctaa atggacaaat aaagataaga ttgaagggtc tttagaagaa
     8941 gtcgtaaaag acgcagatgt atttattgga gtttctgtag ctaatgcgct gtcacaagat
     9001 atggttaaga gtatggcaga taatccaatt atatttgcaa tggctaatcc aaatcctgaa
     9061 ataatacctg atgatgccaa agcggcaggt gcacgagtag ttggtacagg acgttcagac
     9121 tatcctaacc aaattaataa tgtattagct ttccctggta tttttagagg tgcattagag
     9181 gttgaagcta cacatataaa tgaagaaatg aaaaaggcag ctgtagaagc gattgctgat
     9241 ttaatcgata gttctgaatt aaatgaagac tactgtatac caggaccgtt tgataaacgt
     9301 gtagcgccat cagttgctcg taatgttgct aaagcggcaa tggaatctgg agtagctagg
     9361 attgaagttg atccgcaaga tgtgtatgat aaaacaatga aacttacaga tttacaataa
     9421 aaataattaa cgtttaatta aattgcttaa aagttagcaa taaagagtca ttaaaatgat
     9481 gaaaatgaaa gtttatgata aacattcaac agtcaaacga atataaatca aataaattta
     9541 aacccgtttt taactggtta agttcagttt aaggctctaa atggttagaa cagaggttat
     9601 ttggaggttt tcctatgttt aaagattttt ttaatcgaac aaagaaaaag aaatatctta
     9661 cagtacaaga ctctaaaaat aatgatgtgc ctgcaggtat tatgactaag tgtccaaagt
     9721 gtaagaaaat tatgtacaca aaagaattag ctgaaaattt aaatgtgtgc tttaattgtg
     9781 atcatcatat tgctttaact gcgtataaac gtatagaagc aatttctgat gaaggatcat
     9841 ttacagaatt cgataaggga atgacctctg cgaatccatt agattttcca agttatttag
     9901 aaaaaattga aaaggaccaa caaaagacag gtcttaaaga agcagttgtg actggtacag
     9961 cgcaactaga tggtatgaaa tttggcgttg ctgtcatgga ttcacgtttt agaatgggaa
    10021 gtatgggatc ggttatcggt gaaaagatat gtcgaatcat tgattactgc actgagaacc
    10081 gtttaccatt tattcttttc tctgcaagtg gtggtgcacg tatgcaagaa ggtattattt
    10141 ccttgatgca aatgggtaaa accagtgtat ctttaaaacg tcattctgac gctggactat
    10201 tatatatatc atatttaaca catccaacta ctggtggtgt atctgcaagt tttgcatcag
    10261 ttggtgatat aaatttaagt gagccaaaag cgttgatagg ttttgcaggt cgtcgagtta
    10321 tagagcagac aataaacgaa aaattgccag atgatttcca aactgcagaa tttttattag
    10381 agcatggaca attggacaaa gttgtacatc gtaatgatat gcgtcaaaca ttgtctgaaa
    10441 ttctaaaaat ccatcaagag gtgactaaat aatgttagat tttgaaaaac cactttttga
    10501 aattcgaaat aaaattgaat ctttaaaaga atctcaagat aaaaatgatg tggatttaca
    10561 agaagaaatt gacatgcttg aagcgtcatt ggaacgagaa actaaaaaaa tatatacaaa
    10621 tttaaaacca tgggatcgtg tgcaaattgc gcgtttgcaa gaaagaccta cgaccctaga
    10681 ttatattcca tatatctttg attcgtttat ggaactacat ggtgatcgta attttagaga
    10741 tgatccagca atgattggtg gtattggctt tttaaatggt cgtgctgtta cggttattgg
    10801 acaacaacgt ggaaaagata caaaagataa tatttatcgg aattttggta tggcgcatcc
    10861 agaaggttat cgaaaagcat tacgtttaat gaaacaagct gaaaaattca atcgtcctat
    10921 ctttacattt atagatacaa aaggtgcata tcctggtaaa gctgctgaag aacgtggaca
    10981 aagtgaatct atcgcaacaa atttgattga gatggcttca ctaaaagtac cagttattgc
    11041 gattgtcatt ggtgaaggtg gcagtggagg tgccctaggt attggtattg ccaataaagt
    11101 attgatgtta gagaatagta cttactctgt tatatctcct gaaggtgcag cggcattatt
    11161 atggaaagac agtaatttgg ctaaaattgc agctgaaaca atgaaaatta ctgcccatga
    11221 tattaagcaa ttaggtatta tagatgatgt catttctgaa ccacttggcg gtgcacataa
    11281 agatattgaa cagcaagctt tagctattaa atcagcgttt gttgaacagt tagattcact
    11341 tgagtcatta tcacgtgatg aaattgctaa tgatcggttt gaaaaattca gaaatatcgg
    11401 ttcttatata gaataatcaa cttgagcatt tttatgttaa atcgatactg ggttttac
//
LOCUS       DAHHRR010000085        11309 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.085, whole genome shotgun sequence.
ACCESSION   DAHHRR010000085 DAHHRR010000000
VERSION     DAHHRR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.085"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            158..1846
                     /gene="ilvD"
                     /locus_tag="NBI42_001892"
     CDS             158..1846
                     /gene="ilvD"
                     /locus_tag="NBI42_001892"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCD1778304.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            1874..3643
                     /gene="ilvB"
                     /locus_tag="NBI42_001893"
     CDS             1874..3643
                     /gene="ilvB"
                     /locus_tag="NBI42_001893"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCD1778305.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETVEHSDWVKHCQNNKQKHPFKLGEEDS
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVNPMVPSGKSNHEMEGL"
     gene            3643..3897
                     /locus_tag="NBI42_001894"
     CDS             3643..3897
                     /locus_tag="NBI42_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCD1778306.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTIECYDLVDNEA"
     gene            4019..5038
                     /gene="ilvC"
                     /locus_tag="NBI42_001895"
     CDS             4019..5038
                     /gene="ilvC"
                     /locus_tag="NBI42_001895"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCD1778307.1"
                     /translation="MEEFIMTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDN
                     GYDVVIGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNL
                     EKHNALAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQD
                     ASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETL
                     VEAGYQPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVK
                     ENMKAVLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKS
                     KSIEK"
     gene            5068..6597
                     /locus_tag="NBI42_001896"
     CDS             5068..6597
                     /locus_tag="NBI42_001896"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCD1778308.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVISSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTVLNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6600..7646
                     /gene="leuB"
                     /locus_tag="NBI42_001897"
     CDS             6600..7646
                     /gene="leuB"
                     /locus_tag="NBI42_001897"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCD1778309.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLIKLRKSLNLFAN
                     IRPTTVVKGASSLSPLKQERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7660..9030
                     /gene="leuC"
                     /locus_tag="NBI42_001898"
     CDS             7660..9030
                     /gene="leuC"
                     /locus_tag="NBI42_001898"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCD1778310.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            9031..9603
                     /gene="leuD"
                     /locus_tag="NBI42_001899"
     CDS             9031..9603
                     /gene="leuD"
                     /locus_tag="NBI42_001899"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCD1778311.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9618..10886
                     /gene="ilvA"
                     /locus_tag="NBI42_001900"
     CDS             9618..10886
                     /gene="ilvA"
                     /locus_tag="NBI42_001900"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HCD1778312.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKSYSPNTKIIGVEPSGA
                     SSMYESVVVNNQVITLPNIDKFVDGASVARVGDITFEIAKKNVDNYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCIISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
BASE COUNT         3790 a         1879 c         2172 g         3468 t
ORIGIN      
        1 aaatatttac taaacgcttt aagtcctatt tctgtttgaa tgggacttgt aaacgtccca
       61 ataatattgg gacgtttttt tatgttttat ctttcaatta cttattttta ttactataaa
      121 acatgattaa tcattaaaat ttacggggga atttactatg cgaagcgaca tgatcaaaaa
      181 aggagatcac caagcaccag caagaagtct tttacatgcc acgggcgcgc ttaaaagtcc
      241 aactgatatg aacaaaccat ttgtagctat ttgtaactct tatattgata tcgttccagg
      301 acatgttcat ttaagagagc ttgcagatat agctaaagaa gcaattagag aagccggtgc
      361 cattccattt gaattcaata caattggtgt tgatgatgga atagctatgg gacatatcgg
      421 aatgcgatat tctctaccat cacgtgaaat tattgcagat gcagctgaaa ctgtaattaa
      481 cgctcattgg tttgacggcg tattttacat tcctaattgt gacaagatta cacccggtat
      541 gattttagca gccatgagga caaacgtacc agctatcttt tgctctggtg gaccaatgaa
      601 agctggctta tctgcacatg gtaaagcatt aacactttca tcaatgtttg aagcagtcgg
      661 cgcatttaaa gaaggatcga tttctaaaga agaattttta gatatggaac aaaatgcctg
      721 ccctacttgt ggttcatgtg ctgggatgtt tactgcaaat tcaatgaact gtttgatgga
      781 agttttaggt ctagcattac catacaacgg tactgcactt gcagtcagtg atcagcgacg
      841 cgaaatgatt cgccaagcag cttttaaatt agttgaaaat attaaaaatg atttaaaacc
      901 acgtgatatc gttactcgcg aagcaattga tgatgcattt gcacttgata tggctatggg
      961 tggttcaaca aacacggtac tgcatacgtt agccattgcc aatgaagctg gtattgatta
     1021 tgacttagag cgcattaatg ctattgccaa acgcacgcca tatttatcaa aaatagcacc
     1081 tagttcatcg tattcaatgc atgatgtgca tgaagctggt ggcgtcccag caattattaa
     1141 tgaattgatg aagaaagatg gcacattaca cccagataga atcacagtta ctggcaaaac
     1201 gttacgtgaa aataacgaag gcaaagaaat taagaacttt gatgtcattc accctcttga
     1261 tgcaccatat gatgcacaag gtggtttatc tatcttattt ggtaatatcg cccctaaagg
     1321 cgcagttatt aaagttggcg gcgttgatcc atctatcaaa acatttactg ggaaagcaat
     1381 ttgtttcaat tcgcatgatg aagctgttga agcaatagac aatcgtaccg ttcgtgcagg
     1441 ccacgtcgtt gtcattagat atgaaggacc taaaggtgga ccaggtatgc ctgaaatgtt
     1501 agcacctact tcctctattg ttggtcgtgg cttaggtaaa gatgttgcat taattactga
     1561 tggtcgtttt tccggtgcca caagaggtat tgcagttggt catatttccc ctgaagctgc
     1621 atctggtgga ccaattgcct taattgaaga tggagatgag attactattg acttaacaaa
     1681 tcgtacatta aacgtaaacc agcctgaaga tgttctggcg cgtcgccgag aatctttaac
     1741 accatttaaa gcgaaagtaa aaacaggtta tctagctcgt tatactgccc tagtaactag
     1801 cgcaaataca ggtggcgtca tgcaagtgcc tgagaattta atttaattta tttttatatt
     1861 ggagatggtt aaaatgtcta aaactcaaca tgaagtaaac caaaatattg accctttaaa
     1921 aatggctgaa tcacttgaac ctgaacaact aaatgaaaaa actttaaatg acatgcgttc
     1981 aggatcagaa gtgctagtag aagctctact taaagaaaat gtggattatt tattcggtta
     2041 tcctggtggt gccgtactac ctttatacga cacattttat gatggtaaaa tcaaacatat
     2101 tttagcaaga cacgaacaag gtgctgttca tgctgcagaa ggttatgcac gtgtatctgg
     2161 taaaactggc gtcgttgtag ttacaagcgg tccaggtgca acaaatgtaa tgacaggtat
     2221 tacggatgca cattgcgact ctttacctct agttgtattc actggacaag ttgctacacc
     2281 aggtattggt aaagatgcat tccaagaagc ggatattcta tctatgactt caccaattac
     2341 aaaacaaaat tatcaagtga aacgtgttga agatatccct aaaatcgtac acgaagcttt
     2401 ccatgtagct aattctggac gcaaaggtcc tgtagtgatc gattttccaa aagatatggg
     2461 tgttttagct acaaatgtgg atttatgcga cgaaatcaat attccaggtt atgaagttgt
     2521 tacagaacca gaaaataaag acattgacac tttcatctca cttttaaaag aagcgaaaaa
     2581 gcctgtcgta ttagccggcg caggtattaa tcaatcaaaa tcaaatcaat tattaacaca
     2641 gtttgttaat aaacatcaaa tcccaacagt tactacttta ctaggtctag gtgctgtacc
     2701 ttatgaggat acactatttt taggtatggg aggtatgcat ggttcttatg ctagtaatat
     2761 ggcgttaact gagtgtgatt tactcattaa tttaggtagc cgcttcgatg atagattagc
     2821 aagcaaacct gatgcctttg cacctaacgc caaaattgta catgtagata ttgacccttc
     2881 agaaattaat aaagttattc atgtagattt aggtattatt gcagactgta aacgattttt
     2941 agaatgttta aatgataaaa atgttgagac tgtagaacac agtgactggg ttaaacattg
     3001 tcaaaataat aagcagaaac acccatttaa acttggtgaa gaagattcag tattttgtaa
     3061 gccacaacaa acaatcgaat atatcggcaa aattacaaat ggtgaagcaa ttgttactac
     3121 agacgtggga caacatcaaa tgtgggcagc tcaattttat ccatttaaaa atcacggaca
     3181 atgggttaca agcggtggtt taggaacaat gggattcggt attccttcgt caattggtgc
     3241 caaattagct aatcctgata aaacagtcgt atgtttcgtc ggtgacggtg gtttccaaat
     3301 gacaaaccaa gaaatggcac tattacccga atatggttta gatgtcaaaa tcgtactaat
     3361 caataatgga acattaggta tggttaaaca atggcaagat aagttcttta atcaacgctt
     3421 ctcacactca gtatttaatg gtcaacctga ttttatgaaa atggcagaag catatggcgt
     3481 caaaggtttc ttaatcgata agccagaaca actggaagaa caattagatg cagcgtttgc
     3541 ttatcaagga ccagctttaa ttgaggttcg tatttcccct actgaagctg taaacccaat
     3601 ggttccgagt ggcaaatcaa atcatgaaat ggagggctta taatgacaag aattcttaaa
     3661 ttacaagttg cggatcaagt cagcacgcta aatcgaatta caagtgcttt tgttcgccta
     3721 caatataata tcgatacatt acatgttaca cattctgaac aacccgggat ttctaacatg
     3781 gaaattcaag tcgatattca agatgataca tcacttcata ttttaattaa aaaattaaaa
     3841 caacaaatta atgttttaac gattgaatgc tacgaccttg ttgataacga agcttaattt
     3901 taagacaaag gcaatgatgc gctaattagt taaagatata tcataggctg ctagttaaca
     3961 tctgccacta ttacaaagtt atatttcaga attttcgaaa aacaaaacat ttaattattt
     4021 ggaggaattt attatgacaa cagtttatta tgatcaagat gtaaaaacgg acgctttaca
     4081 aggcaaaaaa attgcagtag taggttatgg atcacaaggc cacgcgcatg cacaaaactt
     4141 aaaagacaat ggatatgatg ttgtcatcgg tattcgacca ggtcgttctt ttgacaaagc
     4201 taaagaagat ggatttgatg tgttccctgt tgcagaagca gttaagcaag ctgatgtaat
     4261 tatggtgcta ttacctgatg aaattcaagg tgatgtatat aaaaacgaaa ttgaaccaaa
     4321 tttagaaaaa cataatgcgc ttgcatttgc tcatggcttt aacattcatt ttggtgttat
     4381 tcaaccacca gctgatgttg atgtattttt agtagctcct aaaggaccgg ggcatttagt
     4441 tagacgtaca tttgttgaag gttctgctgt accatcacta tttggtattc aacaagacgc
     4501 ttcaggtcaa gcacgtaata ttgctttaag ttatgcaaaa ggtattggtg caactcgtgc
     4561 aggtgttatt gaaacaacat ttaaagaaga aacagagaca gatttatttg gtgaacaagc
     4621 agtactttgc ggtggtgtat cgaaattaat tcaaagtggc tttgaaacat tagtagaagc
     4681 gggttatcaa ccagaattag cttattttga agtattacat gaaatgaaat taatcgttga
     4741 tttgatgtat gaaggcggta tggaaaatgt acgttactca atttcaaata ctgctgaatt
     4801 tggtgactat gtttcaggac cacgtgttat cacaccagat gttaaagaaa atatgaaagc
     4861 tgtattaact gatatccaaa atggtaactt cagtaatcgc tttatcgaag acaataaaaa
     4921 tggattcaaa gaattttata aattacgcga agaacaacat ggtcatcaaa ttgaaaaagt
     4981 tggtcgtgaa ttacgcgaaa tgatgccttt tattaaatct aaaagcattg aaaaataaga
     5041 tagacctaca atgaggagtt gttaaatatg agtagtcata ttcaaatttt tgatacgaca
     5101 ctaagagatg gtgaacaaac accaggagtg aattttactt ttgatgaacg cttgcgtatt
     5161 gcattgcaat tagaaaaatg gggtgtagat gttattgaag ctggatttcc tgcttcaagt
     5221 acaggtagct ttaaatctgt tcaagcaatt gcacaaacat taacaacaac ggctgtatgt
     5281 ggtttagcta gatgtaaaaa atctgacatc gatgctgtat acgaagcaac aaaagatgca
     5341 gcgaagccgg tcgtgcatgt ttttatagca acatcaccta ttcatcttga acataaactt
     5401 aaaatgtctc aagaagacgt tttagcatct attaaagaac atgtcacata cgcgaaacaa
     5461 ttatttgacg ttgttcaatt ttcacctgaa gatgcaacgc gtactgaatt accattctta
     5521 gtgaaatgtg tacaaactgc cgttgacgct ggagctacag ttattaatat tcctgataca
     5581 gtcggctaca gttaccatga tgaatatgca catattttca aaaccttaac agaatctgta
     5641 atatcttcta atgaaattat ttatagtgct cattgccatg acgatctagg aatggctgtt
     5701 tcaaatagtt tagctgcaat tgaaggcggt gcgagacgaa ttgaaggcac tgtaaatggt
     5761 attggtgaac gagcaggtaa tgcagcactt gaagaagtcg cgcttgcact atacgttcga
     5821 aatgatcatt atggtgctca aactgtcctt aatctcgaag aaactaaaaa aacatcggat
     5881 ttaatttcaa gatatgcagg tattcgagtg cctagaaata aagcaattgt tggccaaaat
     5941 gcatttagtc atgaatcagg tattcaccaa gatggcgtat taaaacatcg tgaaacatat
     6001 gaaattatga cacctcaact tgttggtgta agcacgactg aacttccatt aggaaaatta
     6061 tctggtaaac acgccttctc agagaagtta aaagcattag gttatgacat tgataaagaa
     6121 gcgcaaatag atttatttaa acaattcaag gccattgcgg acaaaaagaa atctgtttca
     6181 gatagagata ttcatgcgat tattcaaggt tctgagcatg agcatcaagc actttataaa
     6241 ttggaaacac tgcaactaca atatgtctct agcggccttc aaagtgctgt tgttgttgtt
     6301 aaagataaag agggtcatat ttaccaggat tcaagtattg gtactggttc aatcgtagca
     6361 atttacaatg cagttgatcg tattttccaa aaagaaacag aattaattga ttatcgtatt
     6421 aattctgtca ctgaaggtac tgatgcccaa gcagaagtac atgtaaattt attgattgaa
     6481 ggtaagactg tcaatggctt tggtattgat catgatattt tacaagcctc ttgtaaagca
     6541 tacgtagaag cacatgctaa atttgcagct gaaaatgttg aaaaggtagg taattaatta
     6601 tgacttataa cattgttgcc ctacctggtg atggaatcgg tccagaaatt ttgaacggat
     6661 ctctatcatt gcttgaaatt ataagtaata aatataactt taattatcaa atagagcacc
     6721 acgaatttgg tggtgcctct attgatacat tcggcgagcc tttaactgag aaaaccttaa
     6781 atgcgtgtaa aagagcagat gctattttac tgggtgcaat cggtggacct aaatggacag
     6841 atcctaacaa tcgaccagaa caaggattaa taaaattacg taaatcatta aatttatttg
     6901 caaatatacg ccccactacc gttgtcaaag gcgctagttc tttatcacct ttaaagcaag
     6961 aacgcgttga aggcacagat ttagttatag tccgtgaatt gacaagtggt atttattttg
     7021 gagaacctag acattttaat aatcacgagg ccttagattc tcttacttat acaagagaag
     7081 aaatagaacg cattgttcac gtagcattta aattggccgc ttcaaggcga ggaaaactaa
     7141 catcagttga taaagaaaat gtattagctt ctagtaaatt gtggcgcaaa gtcgtaaatg
     7201 aagtgagtca attatatcca gaagtaacag taaatcactt acttgttgat gcttgtagta
     7261 tgcatttaat cacaaatcca aaacaatttg acgtcatcgt ttgtgaaaac ttatttggtg
     7321 atattttaag tgatgaagct tcagtgattc ctggttcact tggtttatca ccttctgcta
     7381 gttttagtaa cgatggtcca agattatatg agcctattca tggatcagca ccagatattg
     7441 caggtaaaaa cgttgccaat ccatttggaa tgattctatc tttagcgatg tgtttacgtg
     7501 aaagcttaaa tcaaccagat gctgcagatg aattagaaca acatatttat aacatgattg
     7561 aacatgggca aacgacagca gatttaggcg gcaaattgaa tactactgat attttcgaaa
     7621 ttctatctca aaaattgaat cactaagggg gagatgtaaa tgggtcaaac attatttgac
     7681 aaggtgtgga acagacatgt gttatacggg aaattgggcg aaccgcaact attatacatt
     7741 gatttacacc ttatacatga agttacttct cctcaagcat ttgaaggact taggcttcaa
     7801 aacagaaaat taagacgccc agatttaaca tttgcaacac tcgaccacaa tgttcctact
     7861 attgatattt tcaatattaa agatgaaatt gcaaacaaac aaatcacaac attacaaaaa
     7921 aacgccatag attttggggt gcatattttt gatatgggtt ctgatgaaca aggtattgtt
     7981 cacatggtag gacctgagac aggacttaca cagcccggca aaacaatcgt ttgtggtgac
     8041 tctcacacag caacacatgg agcatttggt gctattgcat ttggaattgg aacaagtgaa
     8101 gttgaacatg ttttcgcaac tcaaacgcta tggcaaacaa aacccaaaaa cttaaaaatc
     8161 gatattaatg gtaccttacc aacaggcgtc tatgctaagg acattattct gcatttaatt
     8221 aaaacgtatg gtgttgattt tggtacaggc tatgctttgg aatttactgg cgaaacaatt
     8281 aaaaaccttt caatggacgg tcgaatgact atttgtaaca tggctatcga aggtggtgcc
     8341 aaatacggca taatccaacc tgatgatata acatttgaat atgttaaagg gagaccattt
     8401 gccgataact tcgctaaatc agttgataag tggcgtgagc tatattctga tgacgacgcg
     8461 atatttgatc gtgtaattga acttgatgtt tcaacattag aaccacaagt gacatgggga
     8521 actaatcctg aaatgggtgt taatttcagt gaaccattcc ctgaaatcaa tgatatcaac
     8581 gatcaacgtg cgtatgatta tatggggtta gaaccaggac aaaaagctga agatatcgat
     8641 ttagggtatg ttttcctcgg ttcatgtaca aatgctagac tatcagattt gattgaagct
     8701 agtcatattg ttaaaggaaa taaagttcac ccaaatatta cagctattgt cgtaccaggt
     8761 tctcgtacag taaaaaaaga agctgaaaaa ttaggtctag atactatttt taaaaatgca
     8821 ggatttgaat ggcgtgaacc aggatgttca atgtgtttag gcatgaatcc tgaccaagta
     8881 cctgagggtg tacattgtgc atctacaagt aatcgaaact tcgaaggacg acaaggcaaa
     8941 ggtgctagaa cacatttagt atcccctgct atggcagcag cagcagctat tcatggtaaa
     9001 tttgtggacg taagaaaggt ggttgtttaa atggcagcaa tcaaacctat tacaacatat
     9061 aaaggtaaaa tagtccctct tttcaacgat aatatcgata cagatcaaat cattcctaaa
     9121 gtacacttaa agcgtatttc aaaaagtggc tttggtccat ttgcttttga tgaatggcgg
     9181 tacttacctg atggttcaga taatcctgat ttcaatccaa ataagcctca atataaaggt
     9241 gcatccatat taattactgg agataatttc ggatgtggtt caagtcgtga acatgcagct
     9301 tgggcactta aggactatgg ctttcatatt attattgcag gaagtttcag tgacattttt
     9361 tatatgaatt gtacaaaaaa tgcaatgtta ccaattgttt tagaaaaaaa tgcacgtgaa
     9421 catcttgcga aatatgttga aattgaggtc gatttaccaa atcaaactgt gtcatcacca
     9481 gacaagcgtt tccattttga aattgatgaa acttggaaga ataaacttgt aaatggctta
     9541 gatgacattg caatcaccct acaatatgaa tcattaatag aaaaatatga aaaatcactt
     9601 taagggagtt gaatattatg acagtcaaaa caacagtttc tacgaaagat atcgatgaag
     9661 catttttaag acttaaagat attgtcaaag aaacaccttt acaattagac cattacttat
     9721 ctcaaaagta tgattgtaaa gtttatttaa aacgagaaga tttacaatgg gtacgttctt
     9781 ttaaattaag aggtgcttac aacgctattt ctgttttatc agatgaagct aaaagtaaag
     9841 gtattacatg tgcgagtgca ggtaatcatg ctcaaggtgt tgcctataca gctaaaaaac
     9901 ttaatttaaa cgctgttatc tttatgccag tcactacacc tttacaaaag gtaaatcaag
     9961 taaagttctt tggaaatagt aacgttgaag ttgtactcac tggtgataca tttgatcact
    10021 gtttagctga agctttaact tatacaagtg aacatcaaat gaactttata gatccattca
    10081 ataatgttca tacaatttct ggacaaggta cgcttgctaa agaaatgcta gaacaagcaa
    10141 agtctgacaa tgttaacttt gattatctat ttgccgcaat tggtggtggt ggtttaattt
    10201 caggtattag tacctatttt aaatcctatt ctcccaacac aaaaattatc ggagttgaac
    10261 cttcaggtgc tagtagtatg tatgaatctg ttgttgtaaa taatcaggtt atcacattac
    10321 ctaatatcga taaatttgtc gatggagctt ctgtagccag agttggcgat attacttttg
    10381 aaattgccaa aaaaaatgta gataactacg tacaagtaga tgaaggtgct gtatgttcaa
    10441 caattttgga tatgtattca aaacaagcaa tagtagcaga acccgctggc gctttaagtg
    10501 taagtgcgct tgaaaactat aaagatcata ttaaaggtaa aacagtggtt tgtatcatta
    10561 gtggtggtaa taatgatatt aatcgaatga aagaaattga agaacgttca ttactatacg
    10621 aagaaatgaa gcattacttt atcttaaatt tccctcaacg tccaggtgca ttgagagaat
    10681 ttgtaaatga cgtattagga cctcaagacg atattactaa atttgaatac ttaaaaaaat
    10741 cttctcaaaa tacaggtact gtcattattg gtattcaact taaagatcat gatgatttaa
    10801 tacaactcaa acaacgtgta aatcatttcg atccttccaa tatttatatt aatgaaaata
    10861 agatgttata ttcattgtta atttaacaca tattaagaaa agtagtcata aattgatttc
    10921 taattgaaat catcttatga ctgcttttta ttatgcttta cattatttag gttgcacgtt
    10981 ttgtatacca tcgtatattt ctttgagcct aaaatcttct ttacctttac ttttttactt
    11041 tgctacttta gacattaaaa aagagacctt gcggtctcaa cttgcctggc aacgttctac
    11101 tctagcggaa cgtaagttgg ctaccatcgt cgctaaagaa ctttcttgac ttgtgacaat
    11161 cgcttgcttc tttcctcttc ttcggctctc gcttactcat ttagctctac taaactcgtt
    11221 gcgctctttt ctcgtttcgt cagattcaaa cgttttcact tcgtcaagcc atttttcttt
    11281 gtgtttgctt tttattttga cgttttaga
//
LOCUS       DAHHRR010000086        11274 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.086, whole genome shotgun sequence.
ACCESSION   DAHHRR010000086 DAHHRR010000000
VERSION     DAHHRR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.086"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..686
                     /gene="tilS"
                     /locus_tag="NBI42_001901"
     CDS             <1..686
                     /gene="tilS"
                     /locus_tag="NBI42_001901"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCD1778313.1"
                     /translation="NQLKVSHLLKLKQWHDEQYDILQYSAKQFIQESVKFDEQSKYLE
                     VSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSINLADKW
                     IIQIAYGKLIIMAKNNGDTYFRVQTIEKPGNYIFNKYRLEIHSNLPKCLFPLTVRTRQ
                     SGDTFKLNGRDGYKKVNRLFIDCKVQQWVRDQMPIVLDKQQRIIAVGDLYQQQTIKQW
                     IIISKNGDE"
     gene            691..1230
                     /gene="hpt"
                     /locus_tag="NBI42_001902"
     CDS             691..1230
                     /gene="hpt"
                     /locus_tag="NBI42_001902"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCD1778314.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            1488..3581
                     /gene="ftsH"
                     /locus_tag="NBI42_001903"
     CDS             1488..3581
                     /gene="ftsH"
                     /locus_tag="NBI42_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCD1778315.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
     gene            3995..4876
                     /gene="hslO"
                     /locus_tag="NBI42_001904"
     CDS             3995..4876
                     /gene="hslO"
                     /locus_tag="NBI42_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCD1778316.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            5055..5987
                     /gene="cysK"
                     /locus_tag="NBI42_001905"
     CDS             5055..5987
                     /gene="cysK"
                     /locus_tag="NBI42_001905"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCD1778317.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            6203..7006
                     /gene="folP"
                     /locus_tag="NBI42_001906"
     CDS             6203..7006
                     /gene="folP"
                     /locus_tag="NBI42_001906"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCD1778318.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAVTRVKAMMDEGADII
                     DVGGVSTRPGHEMVSLEEELNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDII
                     NDQWAGLYDHHMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            6984..7349
                     /gene="folB"
                     /locus_tag="NBI42_001907"
     CDS             6984..7349
                     /gene="folB"
                     /locus_tag="NBI42_001907"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCD1778319.1"
                     /translation="MQDTIFLKGMRFYGYHGVLSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            7346..7822
                     /gene="folK"
                     /locus_tag="NBI42_001908"
     CDS             7346..7822
                     /gene="folK"
                     /locus_tag="NBI42_001908"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCD1778320.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYEGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRVRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVIEPRSKLKVKDLVFVDDSVKRYK"
     gene            8219..8311
                     /locus_tag="NBI42_001909"
     CDS             8219..8311
                     /locus_tag="NBI42_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778321.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            8364..9851
                     /gene="lysS"
                     /locus_tag="NBI42_001910"
     CDS             8364..9851
                     /gene="lysS"
                     /locus_tag="NBI42_001910"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCD1778322.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEYGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            10409..10523
                     /gene="rrf"
                     /locus_tag="NBI42_001911"
     rRNA            10409..10523
                     /gene="rrf"
                     /locus_tag="NBI42_001911"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            10536..10611
                     /locus_tag="NBI42_001912"
     tRNA            10536..10611
                     /locus_tag="NBI42_001912"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10569..10571,aa:Val,seq:tac)
     gene            10628..10703
                     /locus_tag="NBI42_001913"
     tRNA            10628..10703
                     /locus_tag="NBI42_001913"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10661..10663,aa:Thr,seq:tgt)
     gene            10710..10782
                     /locus_tag="NBI42_001914"
     tRNA            10710..10782
                     /locus_tag="NBI42_001914"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10743..10745,aa:Lys,seq:ttt)
     gene            10816..10890
                     /locus_tag="NBI42_001915"
     tRNA            10816..10890
                     /locus_tag="NBI42_001915"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10848..10850,aa:Gly,seq:gcc)
     gene            10898..10986
                     /locus_tag="NBI42_001916"
     tRNA            10898..10986
                     /locus_tag="NBI42_001916"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10932..10934,aa:Leu,seq:taa)
     gene            10992..11068
                     /locus_tag="NBI42_001917"
     tRNA            10992..11068
                     /locus_tag="NBI42_001917"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11026..11028,aa:Arg,seq:acg)
     gene            11089..11162
                     /locus_tag="NBI42_001918"
     tRNA            11089..11162
                     /locus_tag="NBI42_001918"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11123..11125,aa:Pro,seq:tgg)
     gene            11186..11261
                     /locus_tag="NBI42_001919"
     tRNA            11186..11261
                     /locus_tag="NBI42_001919"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11219..11221,aa:Ala,seq:tgc)
BASE COUNT         3968 a         1569 c         2340 g         3397 t
ORIGIN      
        1 ataatcaact taaagtatcg catttattaa aattaaaaca atggcatgat gaacaatatg
       61 atattttgca atattcagct aaacaattta ttcaagaatc tgtgaagttt gatgaacagt
      121 caaaatattt agaggtttct agacaagctt ttaataactt gccaaactca ttaaagatgg
      181 ttgtgttgga ttgcctatta tcaaagtatt atgagttgtt taatattagt gctaaaacat
      241 acgaagagtg gtttaaacaa tttagtagta agaaagcaca attcagtatt aatctcgcgg
      301 ataaatggat aattcaaatc gcatatggta aattaataat aatggctaaa aataatggcg
      361 atacatattt tagagttcaa actattgaaa agccaggtaa ttatattttt aacaaatatc
      421 gattagagat acattctaat ttaccaaaat gtttatttcc gcttacagtg agaacacgac
      481 aaagtggcga tacatttaaa ctgaatgggc gcgatggtta taagaaagtg aatcgcctgt
      541 ttatagattg taaagtgcaa cagtgggttc gggatcaaat gccaatcgta ttggataaac
      601 aacagcgcat tattgcggta ggagatttat atcaacaaca aacaataaaa caatggatta
      661 taattagtaa aaatggagat gaatagcgtt atgcataatg atttgaaaga agtattgtta
      721 actgaagaag atattcaaaa tatctgtaag gaattgggag cacaattaac aaaggattat
      781 caaggtaaac cattagtatg cgtgggtatc ttaaaaggct cagcaatgtt tatgtcagat
      841 ttaattaaac gaattgatac tcatttatca attgatttca tggatgtttc tagttatcac
      901 ggaggcactg agtcaactgg tgaagttcaa atcattaaag atttaggttc ttctattgaa
      961 aataaagacg tattaattat tgaagatatc ttagagactg gtactacact taagtcaatt
     1021 actgaattat tacaatctag aaaagttaat tcattagaaa tagttacttt attagataag
     1081 ccaaaccgtc gtaaagcgga cattgaagct aagtatgtag gtaaaaaaat accagatgaa
     1141 tttgttgttg gttacggttt agattatcgt gaattatacc gaaacttacc atatatcggc
     1201 acgttaaaac ctgaagtgta ttcaaattaa ttttttaatc gatttcagtt attattacta
     1261 tgcgtttgag aaataatagt gtagactcaa aaatatgaaa aatgtatttc atatatattt
     1321 aattttagac aagacatatg tcttaaaaag ttgaaaaata tagagattga taaaactaac
     1381 acagggtgtg aatgacattg atgttaagct caattactag cttataaaac atgtcatatg
     1441 ttacaatttt tgttagtttt attatgggaa gtaggaggaa atgacgcatg cagaaagctt
     1501 ttcgcaatgt gctagttatc gtaataatag gcgttattat ttttggtcta ttttcatatt
     1561 taaacggtaa tggaaatatg ccgaaacagc ttacatataa tcaatttact gagaagttgg
     1621 aaaaaggtga ccttaaaact ttagaaatcc aaccacaaca aaatgtctat atggtaagtg
     1681 gtaaaacgaa aaatgatgaa gactattcat caactatttt atataacaac gaaaaagaat
     1741 tacaaaaaat tactgatgct gctaaaaagc aaaacggtgt aaaattaacg attaaagaag
     1801 aagaaaaaca aagtgtcttt gtgagtatac tttcaacatt aattccagtt gtagtcatag
     1861 cgttattatt tattttcttc ctaagccaag cacaaggtgg cggtagtggc ggtcgtatga
     1921 tgaactttgg taaatctaaa gcaaaaatgt acgataataa taaacgtcgt gttcgtttct
     1981 ctgatgtagc aggggcagat gaagaaaaac aagaattaat tgaaattgtt gatttcttga
     2041 aagataataa aaaattcaaa gaaatgggat ctaggattcc taaaggtgtc ttacttgttg
     2101 gacctccagg tactggtaaa acattacttg ctagagcggt tgcaggtgaa gcaggcgcac
     2161 cattcttctc tattagtggt tcagactttg tagagatgtt tgttggtgtt ggtgcgagcc
     2221 gtgttcgtga cttattcgat aatgctaaga aaaacgcgcc ttgtatcatc tttatcgatg
     2281 agattgatgc tgttggtcgt caacgtggtg caggtgttgg tggcggtcat gatgaacgtg
     2341 aacaaaccct aaaccaatta ttagttgaaa tggatggatt cggtgaaaat gaaggtatca
     2401 ttatgatagc tgctacaaac cgtcctgata tccttgaccc agccttatta cgtccaggtc
     2461 gttttgatag acaaattcaa gttggtcgtc cagatgtgaa aggccgtgaa gcaattcttc
     2521 atgttcatgc taaaaataaa ccacttgatg aaacggttga tttaaaagca atttcacaac
     2581 gtacacctgg tttctcaggt gctgatttag agaacttatt aaatgaagca tctttaattg
     2641 ctgtacgtga aggtaaaaag aaaattgaca tgagagatat cgaagaggcg acggatagag
     2701 ttatagccgg acctgctaag aaatctcgag ttatttctaa gaaagaacgt aatattgttg
     2761 ctcatcacga agctggtcat acaattatcg gtatggtact tgatgaggca gaagtagtgc
     2821 ataaagttac tattgttcca cgtggacaag caggtggata tgcaatgatg ctacctaaac
     2881 aagatcgttt cttaatgact gaacaagagt tattagataa aatctgtggt ttacttggtg
     2941 gacgtgtatc agaagatatt aactttaacg aagtatcaac aggtgcttca aatgacttcg
     3001 aacgtgcaac acaaatcgca cgctcaatgg ttacgcaata tggtatgagt aaaaaattag
     3061 gaccattaca gttcggtcat agcaatggtc aagtattctt aggtaaagat atgcaaggtg
     3121 agcctaatta ttcaagccaa atcgcatatg aaattgataa agaagttcaa cgaatcgtta
     3181 aagaacaata cgaacgttgt aaacaaattt tattagagca caaagaacaa ttaattttaa
     3241 ttgctgaaac attattaaca gaagaaacat tagttgctga acaaattcaa tcattattct
     3301 acgaaggtaa attacctgaa attgattatg atgcagctaa agttgttaaa gatgaagatt
     3361 ctgaatttaa tgatggtaaa ttcggtaaat cttatgaaga gattcgtaaa gagcaattag
     3421 aagatggaca acgtgacgaa agtgaagatc gtaaagaaga aaaagatatt gctgaggata
     3481 aaaaagaagc tgataaatct gatgaaaaag acgaaccagc acatcgacaa gccccaaata
     3541 tcgaaaaacc ttacgatcca aatcacccag acaataaata accgattata ttcagtacct
     3601 ctttctatga taaagttata gaaagaggta cttttatgat ttaaggaata cgagcctgag
     3661 acataaatca atgttcaatg ctctacaaag ttatattaac agtagttgac tgaataaaaa
     3721 tacacttgta acaagctttt ttcaaaaata gtcggggccc caacacagaa gctgacgaaa
     3781 agtcagctta cgataaagtg caagttgggg tgggacgacg aaataaattt ttcgaaaata
     3841 tcatttctgt cccactccca ttatggcacg aataattaat aatcaaacaa gttgcttcat
     3901 tatttagaat gatgggtgta gaataagtac aattgttgca ttttatgaag taaagtaatt
     3961 ttttaaatat agagtaatag aggagattga aataatgaca cacgattata ttgttaaagc
     4021 attagcattt gatggagaga ttagggctta tgctgctttg acaactgaaa ctgttcaaga
     4081 agcacaaacg agacattata catggccgac agcatctgct gcaatgggaa gaacaatgac
     4141 agcaacagct atgatgggcg caatgttgaa aggtgatcaa aaattaactg tcactgtaga
     4201 tggccaagga cctattggac gaattattgc cgatgcaaat gctaaaggcg aggtgcgtgc
     4261 ttatgtagac catccacaaa ctcattttcc attaaacgag caaggtaaac ttgatgtaag
     4321 acgagcagta gggacaaatg gatctattat ggttgttaaa gacgttggaa tgaaagacta
     4381 tttctctgga gcaagtccaa ttgtttcagg agaacttggt gaagatttta cttattatta
     4441 tgctacaagt gaacaaacac cttcatcggt aggtcttggt gtattggtaa atcctgataa
     4501 tacgattaaa gcagcaggag gatttatcat tcaagttatg ccaggtgcca aagatgaaac
     4561 aatttcaaaa ttagaaaaag caattagtga aatgacacca gtttctaaat taattgaaca
     4621 aggattaacg ccagaaggat tactaaacga aatcttaggt gaagaccatg tgcaaatttt
     4681 agagaaaatg cctgttcaat ttgaatgtaa ttgtagtcat gagaaatttt taaatgctat
     4741 taaaggattg ggcgaggctg agattcaaaa tatgattaaa gaagatcatg gtgctgaagc
     4801 agtatgtcat ttctgtggaa ataaatataa atatactgaa gaagaattaa acgtgttgct
     4861 agaaagttta gcgtaattta atttaaatca atacgctaaa atgtttattt ttagcggttt
     4921 agtgaaatgt agaactaaat agttgtataa tccttagtaa ttttgtttgc tttctagaat
     4981 ttatttgata aaataattct atatccgata aataaactaa gatttcaaca actaactaaa
     5041 aaggagtgtt cttaatggca caaaaaccag tagataatat tactcaaatt attggcggta
     5101 caccggtagt caaattgaga aatgtagtag atgacaatgc agcagatgtt tatgtaaaat
     5161 tggaatatca aaatccaggt ggttctgtaa aggatagaat tgctttagca atgattgaaa
     5221 aagcagaacg agaaggcaaa attaaacctg gcgatacaat tgtagaacca acaagtggta
     5281 atacaggtat cggtttagca tttgtatgtg ctgctaaagg atataaagca gtatttacta
     5341 tgcctgaaac aatgagccaa gagcgtcgta atttattaaa agcatacggt gcggaattag
     5401 ttttaacgcc tggatcagaa gcgatgaaag gtgcaattaa aaaagctaaa gaattaaaag
     5461 aagaacatgg ttacttcgag ccacaacaat ttgaaaaccc tgcgaaccct gaagttcatg
     5521 agttaactac aggtcctgag ttattacaac aatttgaagg gaaaactatc gatgcgttcc
     5581 tagctggtgt tggtactggt ggtacgttat ctggtgtagg taaagttctg aaaaaagaat
     5641 atcctaacat cgaaattgtt gctatagagc ctgaggcttc tccagtattg agtggtggtg
     5701 agccaggtcc acataaatta caaggtttag gtgctggatt tattccaggt actttgaata
     5761 cagaaatcta tgacagtatt attaaagtag gaaatgatac agcgatggaa atgtctcgtc
     5821 gagttgctaa agaggaaggt attttagcgg gtatttcatc aggtgctgcg atttatgctg
     5881 ccattcaaaa agcaaaagaa ttaggaaaag gtaaaacagt agtaacagta ttgccaagta
     5941 atggtgagcg ctacttatca acacctttat attcattcga tgactaatta atgtcattta
     6001 aaagagtgag ttatcttttt gagataactt gctctttttt tctaccatgt ataattttaa
     6061 aaatatgagc gttaaattaa acatttttct gataaaaaca tccagtgaat gataagataa
     6121 taaacgtaca tactaataac tagtaaatcg caggagtaaa ttttactaga gttaaacaat
     6181 acataattaa agggtggtta acatgactaa aacaaaaatt atgggcatat taaacgtcac
     6241 acctgattca ttctcagatg gtggaaaatt taataatgtt gaatcagcag taactagagt
     6301 gaaagcgatg atggatgaag gtgctgacat tatagatgtt ggaggtgttt caacgagacc
     6361 cggccatgaa atggtttcat tagaagaaga gctgaacaga gtattacctg ttgttgaagc
     6421 gattgtcggt tttgatgtga aaatttcagt cgatacattt cgaagtgagg ttgctgaagc
     6481 atgtttaaaa ttaggcgttg atatcattaa tgatcaatgg gcggggctgt atgatcatca
     6541 tatgttccaa attgtagcta aatatgacgc ggaaattatt ttaatgcata atggaaatgg
     6601 taatcgtgat gaaccggttg tcgaagaaat gttaacatct ttgttagcac aagcacatca
     6661 agctaaaata gctggtatac cttcaaataa aatttggcta gatccaggta taggtttcgc
     6721 taaaactaga aatgaagaag ccgaagttat ggcaagactg gatgaacttg ttgcaacaga
     6781 atatccagtt ttattagcga caagccggaa acgtttcact aaagagatga tgggttatga
     6841 tacaacaccg gttgaaagag atgaagtaac tgcagctacg actgcatatg gtattatgaa
     6901 aggcgttaga gcagtacgcg ttcataatgt cgagttgaat gctaaattag ctaaaggtat
     6961 agatttttta aaggagaatg aaaatgcaag acacaatctt tcttaaaggt atgcgctttt
     7021 atggatatca tggtgtttta tcagctgaaa atgaaatagg gcaaattttc aaagtggatg
     7081 taactttgaa agtagactta gctgaagctg gtcgtactga taatgttatt gatacagttc
     7141 attatggtga agtgttcgaa gaggttaaat caattatgga aggtaaggcc gttaatttac
     7201 ttgagcatct agctgagcgt attgcaaatc gtataaattc acaatataat cgtgtaatgg
     7261 aaacgaaagt gagaatcact aaagaaaacc caccgattcc gggtcattat gatggagtag
     7321 gtatcgaaat agtgagggag aataaatgat tcaagcatac ttaggattag gtagtaatat
     7381 tggtgataga gaaagccagt taaacgatgc tataaagatt ttgaatgaat atgaaggtat
     7441 taacgtatct aatatttctc cgatttatga aacagcacca gttgggtata ctgagcaacc
     7501 taacttttta aatttgtgtg ttgaaattca aacaacactc acagtattac aactgttgga
     7561 atgttgtttg aagacagaag aatgtttaca ccgtgttaga aaagaacgat ggggtcctag
     7621 aactttagat gtggatattt tgttgtatgg agaagaaatg atagatttac caaaactgtc
     7681 ggtgccacat ccgagaatga atgaacgtgc atttgtttta atcccattaa atgatatagc
     7741 agcaaatgtc atagaaccac gttcgaaatt gaaagtgaaa gatttagttt ttgtcgatga
     7801 cagtgtaaag agatataaat aatgtattgt tgagaacatt catatttatt gggaatagat
     7861 gatgattgat tattggagat gtttccgcat tttgattatc tctaaatgca tttgatttcg
     7921 aattagtata tgatgattta gtatgtgtta gaattaagtt atttcataaa atcaaagtaa
     7981 gcaatatttg ataaattgct gtaatttatt tggggttttg ttgaagtttg tggaagataa
     8041 tttttataca tttacttagg gttgtattaa tgataatcgt attttaaatg aataagtaat
     8101 tcataaacga aaacagaaat tgctttatta catttacaac ctcttgtaaa attaaaaaag
     8161 tgataaaatt gatgttatga atataagaaa acatgccaca tcataacttt aaggtgtaat
     8221 ggttaatgat aaagtattag aaacatcgaa agagatgtat gttgagcaaa aatgtctgat
     8281 attttataaa actttaaagg aaaatgtttg agtgtaccag ttggaatact aaaggattac
     8341 aacaagttaa aggagagaaa gttatgtcag aagaaatgaa tgaccaaatg ttggttcgac
     8401 gtcaaaaatt acaagaatta tatgatcttg gtatagaccc gtttggttct aaatttgacc
     8461 gttcaggttt atctagtgat ttgaaagaag agtgggacca gtattctaaa gaagaattgg
     8521 tagaaaaaga agcggatagt catgtcgcta tagctggacg attaatgact aagcgtggta
     8581 aaggtaaagc aggatttgca cacgttcagg acttagctgg acaaattcaa atttacgttc
     8641 gtaaagatca agttggcgat gacgaatttg atttatggaa aaatgctgat ttaggcgata
     8701 tcgttggtgt tgaaggtgta atgttcaaaa caaatactgg cgaattatcg gttaaagcga
     8761 agaaattcac gctactaact aaatcattgc gaccattacc ggataaattc cacggtttac
     8821 aggatattga acagagatat cgtcaaagat atttagattt aattacgaac gaagatagca
     8881 ctcgtacatt tattaatcgt agtaaaatca ttcaagaaat gcgtaattat ttaaataata
     8941 aaggtttctt ggaagtagaa acacctatga tgcaccaaat tgctggtggc gcagctgcta
     9001 gaccatttgt aacacatcat aatgcattag atgcaacgtt atacatgcgt attgctattg
     9061 agttgcattt gaaacgctta attgtcggtg gacttgaaaa agtgtatgaa attggtagag
     9121 tattccgtaa tgaaggtgta tcaactagac ataaccctga attcacaatg attgaattat
     9181 atgaagcata tgcagattat catgacatta tggatttaac agaatctatg gtgagacata
     9241 ttgccaatga agtattaggt tctgcaaaag tacaatacaa tggggaaacg attgatttag
     9301 aatctgcttg gactcgtttg catattgttg atgctgtaaa agaagctact ggtgtagatt
     9361 tttatgaagt taaaagtgat gaagaagcta aagctttagc taaagaatat ggtattgaaa
     9421 ttaaagatac aatgaaatat ggtcatattt taaatgaatt ctttgagcaa aaagttgaag
     9481 aaacacttat tcagccaacg tttatctatg gtcatccgac tgaaatttcc cctttagcga
     9541 agaaaaatcc tgaagatcct agatttactg atcgtttcga attgttcatt gtaggtagag
     9601 agcatgcaaa tgcatttact gaattaaatg atcctattga tcaaaaaggt cgttttgaag
     9661 cgcaacttgt tgaaaaagcg caaggtaatg atgaagcgca tgaaatggat gaagattaca
     9721 ttgaagcgtt agaatatggt atgcctccga caggtggtct tggtatcggt attgacagat
     9781 tagttatgtt attaactgac tctccatcaa tcagagacgt attattattc ccttatatga
     9841 gacaaaaata aatgacgttg attgttagta agagctctcg tgtatacaac atgtgtatgc
     9901 gagggttttc ttaattatgg taattagttc gtgtttgaat gtttttgata gtaatagtta
     9961 acgataatgg tgctattttt gactgttaaa caaggtagtt agctgataga tgaaaatgac
    10021 aatacgtata tagagtagtt ctgatgagaa atgttaacga tagacattga gatatcttat
    10081 aacaaaaatt gctatattag tataatttat cttaatcagc tataaaagta ctttaaaatt
    10141 gtatagaatg tgtatggttt tgtacatatg tgtatgatag aatactaaaa gtgttatgaa
    10201 ttagagagca cgagaaatgt cagttttgaa gaataaaaaa gttaattaaa agtgttgact
    10261 ttatcaattg aatgaagtaa tatataaaag tcgtcaaaaa cagacgaaac acactaaaag
    10321 ccgatgtgac aaagttcaca acaaagtgta aaattaacta ttgcgcctta ttaattaagc
    10381 gtgtatcata aataagtaag ttattttgtc tggtgactat agcaaggagg tcacacctgt
    10441 tcccatgccg aacacagaag ttaagctcct tagcgtcgat ggtagtcgaa cttacgttcc
    10501 gctagagtag aacgttgcca ggcaaatgac aaatcggaga attagctcag ctgggagagc
    10561 atctgcctta caagcagagg gtcggcggtt cgaacccgtc attctccacc atttattctt
    10621 acatatagcc ggcctagctc aattggtaga gcaactgact tgtaatcagt aggttggggg
    10681 ttcaagtcct ctggccggca ccatcttttg agccattagc tcagctggta gagcatctga
    10741 cttttaatca gagggtcaga ggttcgaatc ctctatggct cattacgatt taattcttat
    10801 atttagcaaa ataatgcaga agtagttcag cggtagaata caaccttgcc aaggttgggg
    10861 tcgcgggttc gaatcccgtc ttctgctcca ttattttgcc ggggtggcgg aactggcaga
    10921 cgcacaggac ttaaaatcct gcggtgagag atcaccgtac cggttcgatt ccggtcctcg
    10981 gcaccatttt agcgcccgta gctcaattgg atagagcgtt tgactacgga tcaagaggtt
    11041 atgggttcga ctcctatcgg gcgcgccatt tttaaattaa ttgaataacg ggaagtagct
    11101 cagcttggta gagcacttgg tttgggacca aggggtcgca ggttcgaatc ctgtcttccc
    11161 gattacttct taaattccat tttatggggg cttagctcag ctgggagagc gcctgctttg
    11221 cacgcaggag gtcagcggtt cgatcccgct agtctccacc atttattttc acac
//
LOCUS       DAHHRR010000087        11254 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.087, whole genome shotgun sequence.
ACCESSION   DAHHRR010000087 DAHHRR010000000
VERSION     DAHHRR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.087"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            122..1093
                     /locus_tag="NBI42_001920"
     CDS             122..1093
                     /locus_tag="NBI42_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778323.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKSSSKKSEESKQETVKKES
                     EEEKDPKVAHYKKNKEKIAKVKAVPVLEKIEPFITEKTFMNSTGMQGWKDYKSLMNQV
                     KLADYNFTKESKGASMEDVDKFFKNKKGVKRKEVTTYDGLKQVNYWYVDKSGKKIGGS
                     DTPVFYAEILTKYKDGKLIYASVEPGSYSYSNKNAVNLDKVEELDDLSMFSNLKDPKP
                     VPYSVAQMEISSVPVTSVSFVTKGGNHKDTNPEKEQVDMPQLAYLTVSPQLYHDKDHP
                     DPHIIGLVALPYLNASRDFGNAHYSVLNNLSKEMKEKLASRSLDLDK"
     gene            complement(1231..1434)
                     /locus_tag="NBI42_001921"
     CDS             complement(1231..1434)
                     /locus_tag="NBI42_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCD1778324.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1733..2221
                     /locus_tag="NBI42_001922"
     CDS             1733..2221
                     /locus_tag="NBI42_001922"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCD1778325.1"
                     /translation="MAIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIIRQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTEKEVEAYFI
                     ND"
     gene            2214..3149
                     /locus_tag="NBI42_001923"
     CDS             2214..3149
                     /locus_tag="NBI42_001923"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCD1778326.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDINLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3146..4453
                     /gene="rsmB"
                     /locus_tag="NBI42_001924"
     CDS             3146..4453
                     /gene="rsmB"
                     /locus_tag="NBI42_001924"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCD1778327.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVDLQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4456..5550
                     /gene="rlmN"
                     /locus_tag="NBI42_001925"
     CDS             4456..5550
                     /gene="rlmN"
                     /locus_tag="NBI42_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCD1778328.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5557..6300
                     /locus_tag="NBI42_001926"
     CDS             5557..6300
                     /locus_tag="NBI42_001926"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HCD1778329.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEEHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6297..8291
                     /gene="pknB"
                     /locus_tag="NBI42_001927"
     CDS             6297..8291
                     /gene="pknB"
                     /locus_tag="NBI42_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HCD1778330.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKAETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     MLYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEQALQKLKSLGLKDVKIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVDDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKDDSEDVKSTTASVDVPYTGKNDKPQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDI"
     gene            8519..9394
                     /gene="rsgA"
                     /locus_tag="NBI42_001928"
     CDS             8519..9394
                     /gene="rsgA"
                     /locus_tag="NBI42_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCD1778331.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFDVQNINEGYIHQVYERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARVLVTKKDKTPIEKQLEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9395..10039
                     /locus_tag="NBI42_001929"
     CDS             9395..10039
                     /locus_tag="NBI42_001929"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCD1778332.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            10046..10687
                     /locus_tag="NBI42_001930"
     CDS             10046..10687
                     /locus_tag="NBI42_001930"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCD1778333.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPSGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(11068..>11254)
                     /locus_tag="NBI42_001931"
     CDS             complement(11068..>11254)
                     /locus_tag="NBI42_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCD1778334.1"
                     /translation="GKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKPK
                     KVWVSARALKSGKVTRV"
BASE COUNT         4182 a         1551 c         2105 g         3416 t
ORIGIN      
        1 aattatcaat taaagtattt aaaaatattg atggttaatg taaaatgtca atataaataa
       61 gttttttaaa tttatgtatg tttatttgat tcaaacaaaa taacttaaga ggagaaagtt
      121 tatgaaaaag acactgggat gtttactttt aattatgctt ttagtcgtag caggttgttc
      181 ttttggtggg aatcataaat catcatcaaa gaaatcagaa gaatcaaaac aagaaactgt
      241 aaaaaaagaa tcggaagaag agaaagatcc aaaagttgca cactataaaa aaaataagga
      301 aaaaatagcg aaagttaaag cagtacctgt tttagaaaag atagaaccat ttataaccga
      361 aaagacattt atgaatagca caggtatgca agggtggaaa gattataagt ctttaatgaa
      421 tcaagtgaag ttagcagatt ataattttac aaaagagtct aaaggtgctt caatggaaga
      481 tgttgataag ttttttaaaa ataaaaaagg tgtaaaacgt aaagaagtta ctacttatga
      541 tggtttgaaa caagttaact attggtatgt agataaaagt ggtaaaaaaa ttggtggttc
      601 agacacacct gttttttatg ctgaaatttt aacgaaatat aaagatggaa agttgattta
      661 tgcatcagtt gaacctggat catattcata ttcaaataag aatgcggtaa atctagataa
      721 agttgaagaa cttgatgatt taagtatgtt ctctaatctg aaagatccta aaccagtacc
      781 atatagtgtt gctcaaatgg aaatatcaag cgtcccagtt acaagtgtat ctttcgtaac
      841 aaagggaggg aatcataaag atactaatcc agagaaagaa caagttgata tgccacaact
      901 agcttattta actgtttcac cacagctata ccatgataaa gatcatccag atccgcatat
      961 aattggatta gttgccttgc catacttaaa tgcatctaga gattttggga acgcgcatta
     1021 ctctgtttta aataatcttt caaaagaaat gaaagagaaa ttagcgagtc gttcattgga
     1081 ccttgataaa tagtgtgcta cattaaataa tcgcaataat aatcccgata aacaatcagc
     1141 attactgctt atcatataga gttcgtaata actataactc tatgattcgc aaataataaa
     1201 tgattgtcat cgggatttat ttttatcaat ttataaagtg acattacctt gttcatcagc
     1261 aggtttgaaa acagtaatca ctgcactaat aattgctaaa atgtgtggga tacctgtcca
     1321 acagaatatt aagtgtagaa taccttgcat attcttgccg gcataaaatt tatgaatacc
     1381 aaaactacct aagaacaatg ctaataaaat ataaataact ttgtttactt gcatttcttt
     1441 ccctccagtt gaattgctta taatgacatt agcttctctt tttattatac ctacttttag
     1501 ttcaaacatt ctagtttaag cattcccaat catctaaatt tcagttattc aatccttaca
     1561 ataaatatag tatttcactt cagttgcatt gtattatttt acgtgtgaaa tatacgtaat
     1621 gaatcacatg acaatcttta aattgaaaaa tatacattct atgatgtaag gtcgcatttt
     1681 taatatattt acgttataat ggtttgatga attttattga ggagtaagag ctatggcgat
     1741 taaaaagtta gtaccagcat cgcatcctat tttaacgaaa aaagcgcaag cagttataaa
     1801 atttgatgat tcgttaaaaa gattattaca agatttagaa gatacaatgt atgcacaaga
     1861 agctgctggc ttatgtgcac ctcaaattaa tcagtcattg caagtggcaa tcattgatat
     1921 ggaaatggaa ggattattac aacttgttaa tccgaaaatt attagacaat caaatgaaac
     1981 aataacagac ttagaaggtt caattacatt gccagatgtt tacggcgaag tgacaagaag
     2041 taaaatgatc gttgtcgaaa gttatgacgt caatgggaac aaagttgaac taactgcaca
     2101 tgaagatgta gcaagaatga ttttgcatat tatagatcaa atgaacggta tcccttttac
     2161 agaacgtgcg gaccgtattt taacagaaaa agaagtggag gcatatttta taaatgacta
     2221 aaataatatt tatgggtaca ccagactttt caacaactgt tttagaaatg cttattgcag
     2281 aacatgatgt cattgcagtc gtaacgcaac cagatcgacc tgttggacgt aaacgtgtta
     2341 tgacaccacc accagttaaa aaagttgcaa tgaaatatga tttacctgta tatcagcctg
     2401 aaaaattaag tggatcagaa gaattagaac aattgcttca attagatgta gatttaattg
     2461 taactgctgc ttttggacaa ttattacctg aatcattgtt ggcattacca aaacttggcg
     2521 caattaatgt acatgcatca ttgttaccga agtatagagg tggtgcacca attcatcagg
     2581 caattatcga tggtgaacaa gaaaccggca taacaattat gtatatggtt aaaaaattag
     2641 atgcgggtaa tattatttcg caacaagcaa ttaaaataga agaaaatgat aatgtcggta
     2701 cgatgcatga taaattaagt gtattagggg cagatttatt aaaagaaact ttaccatcta
     2761 ttatagaggg cacaaatgaa agtgtacctc aagatgatac gcaagcaaca tttgcttcca
     2821 atattcgacg cgaagatgag cgaattagct ggaataaacc aggaagacaa gtgtttaatc
     2881 aaattcgagg attatcacca tggccagttg cttatacaac tatggacgat attaatttga
     2941 aaatatatga tgcggaactc gttgagacta ataagataaa cgagcccgga acaattatag
     3001 aaacgactaa aaaagccatt attgttgcta caaatgataa tgaagctgtt gcaattaaag
     3061 atatgcaatt ggctgggaaa aagagaatgt tagctgccaa ttatttaagt ggtgcgcaaa
     3121 acacactagt agggaagaaa cttatatgat agaaaacgtg agaagtcttg cttttgacac
     3181 gattcaagat atattaaatg aaggtgcgta tagtaacttg cgtatcaatg aagtgttgtc
     3241 agaaaatgaa ttaaatgcaa tggataaagc tttatttaca gaaattgtct acggaaccgt
     3301 taaaagaaaa tatacgttag atttttattt aaagcctttt gtgaaaacga agattaaggc
     3361 atgggttagg caattattat ggatgagtat ttatcaatat gtttatttag ataaagttcc
     3421 aaatcacgcc atcattaatg aagcagttga aatagcaaaa gaacgaggtg gctatcacaa
     3481 cggaaatgtc gtaaatggca ttttacgaac aatgatgcgt agtgacttac ctgattttaa
     3541 tgaaattgca gatcctaaga aaagaatggc aatcgagtat agtatgccga agtggattat
     3601 agatcattgg gcaacacatt atggtctcga agaaactgaa acaattttac agtcattttt
     3661 agaaacgaca tcaacaactg tgcgtgccaa cctgacgcga gcatcattag atgatattat
     3721 tgaaaagttg caagacgaag gttatgacgt tgaaaaagat catgacttac cttattgtct
     3781 ccatatagga ggacaaccaa ttattcattc tcgttcattt aaagatggat tcgtttcaat
     3841 tcaagataaa agctcaatgt ttgttgcaca cattatgaat gtagaccgac atgatcacgt
     3901 attagatgca tgtagtgcac ctggcggtaa ggcttgtcac attgctgaag ttttaatgcc
     3961 agaagggcaa gttgacgctt cagatataca tgatcacaaa atagacttaa ttaattttaa
     4021 tataaaaaaa ttacgattaa caaatattaa agcttttcaa catgatgcga caaaacctta
     4081 tgataaaaca tacgataaga tacttgttga tgcaccgtgt agtggattag gtgttatgag
     4141 acacaagcca gagattaaat atactcaaag caaacaacat attgagtcac tagttgattt
     4201 acagcttgaa atattggaaa atgtaaaaaa caatgtaaaa ataggtggcg aaatcatcta
     4261 ttcaacatgt acaattgagc aactagaaaa tgaaaacgtg atttatacgt ttttgaaaaa
     4321 taataaaaac ttcgaatttg aaccgtttca acatccgata actggagagt tggtcaaaac
     4381 gttacaaatc atgccgcaag actttaattc agatggattc tttatcacta agataaaaag
     4441 aaaggacaat taggaatgat aactgctgaa aagaaaaaga agaataaatt tcttccaaat
     4501 tttgacaagc aatcaatata ttcattgcga tttgacgaaa tgcaaaactg gctcgttgaa
     4561 caaggtcaac aaaaatttcg agcgaaacag atttttgaat ggttatatca aaaaagagta
     4621 gactcgattg atgaaatgac gaacttatcg aaagacttac gacagctttt aaaagataac
     4681 tttactgtta caactttaac aactgtagta aaacaagaaa gtaaagacgg tacaattaaa
     4741 ttcttatttg aattacaaga tggctataca attgaaactg ttttaatgag acatgattat
     4801 ggaaattcag tatgtgtaac gacacaagta ggttgtcgta tcggatgtac gttttgtgct
     4861 tctacacttg gcggtttaaa aagaaacctt gaagctggcg aaattgtttc acaagtttta
     4921 acagttcaaa aagcccttga tgctacagaa gagcgcgtat ctcaaattgt cataatgggt
     4981 atcggtgaac catttgaaaa ttatgatgaa atgatggact ttttaagaat cgtcaatgat
     5041 gataacagtt taaatattgg tgcacgtcac attacagtat caacatcagg tatcattcct
     5101 agaatatatg actttgcgga tgaagatatt caaattaatt tcgctgtaag tttacacgcc
     5161 gcaaaagatg aagtgcgatc acgcttgatg ccaattaacc gtgcatataa tgttgagaag
     5221 ttaatcgaag caattcaata ttatcaagaa aaaacaaatc gtcgtgttac ttttgaatat
     5281 ggtctgtttg gtggtgtgaa tgaccaacta gaacatgcaa gagaattagc acatttaata
     5341 aaaggcttaa actgccatgt taacttaatt cctgtcaacc atgttccaga aagaaattat
     5401 gtaaaaacgg ctaaaaatga tatctttaaa tttgaaaaag aattaaagag actaggaatt
     5461 aatgccacaa tacgtcgtga acaaggttcg gatattgacg cagcttgtgg tcaattaaga
     5521 gcaaaggaac gacaagtaga aacgaggtaa agacaaatgc tagaggcaca attttttact
     5581 gatactggac aacatagaga taagaatgaa gatgcgggtg gtatttttta taatcaaact
     5641 aatcaacaac ttttagttct gtgtgatggt atgggcggcc ataaagcagg agaagttgca
     5701 agtaaatttg ttacagatga gttgaaatcc cgttttgaag cggaaaatct tatagaagaa
     5761 catcaagctg aaaattggtt gcgtaataat ataaaagata taaattttca gttatatcac
     5821 tatgcacaag aaaatgcaga atataaaggt atgggtacaa catgtgtttg tgcacttgtt
     5881 tttgaaaaat cagttgtgat agcaaatgtc ggtgattcta gagcctatgt tattaatagt
     5941 cgacaaattg aacaaattac tagtgatcac tcatttgtta atcatcttgt tttaacgggt
     6001 caaattacgc cggaagaagc atttacacat ccacaacgta atattattac gaaggtgatg
     6061 ggcacagata aacgtgtgag tccagatttg tttattaagc gattaaattt ttatgattat
     6121 ttattattaa attcagatgg attaactgat tatgttaaag acaatgaaat taagcgtttg
     6181 ttagtaaaag aaggtacaat agaagatcat ggtgatcaat taatgcaatt ggcattagat
     6241 aaccattcga aagataacgt tactttcata ctcgcggcta ttgaaggtga taaagtatga
     6301 taggtaaaat aataaatgaa cgatataaaa ttgtagataa gcttggcggc ggtggcatga
     6361 gtaccgttta tcttgcagaa gatacgatac ttaacattaa agttgcaatt aaggcgattt
     6421 ttataccacc tagagaaaaa gcagaaacat taaaacgttt tgaacgagaa gtacataact
     6481 catcgcagct atcacatcaa aatatagtaa gtatgatcga tgttgatgaa gaagatgact
     6541 gttactactt agtaatggaa tatattgaag gtccgacttt gtctgagtat attgaaagtc
     6601 atgggccatt aagtgttgac acggcgatta attttacgaa tcaaatattg gatggcatta
     6661 aacatgcgca tgatatgcgt attgtacata gagatattaa gccacaaaat atattaattg
     6721 acagcaataa aacgttgaaa atatttgatt ttggaattgc taaagcttta agtgagacgt
     6781 ctttaactca gactaatcat gtgttaggta ctgtgcagta cttttcgcca gaacaagcaa
     6841 aaggtgaggc aacggatgaa tgtacagata tttattctat aggtattgtg ttatatgaaa
     6901 tgcttgttgg tgaaccacca tttaatggag aaactgcagt tagcattgcg attaaacata
     6961 ttcaggattc tgtgccaaat gtgacaacag atgtacgtaa ggatattccg caatctttaa
     7021 gtaatgtcat tttacgcgct acagaaaaag acaaagcgaa tcgttacaaa acaattcaag
     7081 aaatgaaaga tgatttgagt agtgttttac atgaaaatcg agcgaatgaa gacgtctatg
     7141 aactcgataa aatgaaaacg atagcggtac ctttgaaaaa agaagatcta gcaaagcata
     7201 ttagtgaaca taagtcgaat caacctaaac gtgaaacgac gcaagtacct attgtaaatg
     7261 ggcctgctca tcatcagcaa ttccaaaagc cagaaggtat gttgtatgaa ccaaaaccta
     7321 aaaagaaatc aacacgaaag attgtgctct tatcactaat cttttcgttg ttaatgattg
     7381 cacttgtttc ttttgtggca atggcaatgt ttggtaataa atacgaagag acacctgatg
     7441 taatcgggaa atctgtaaaa gaagcagagc aaatattcaa taaaaacaat ctgaaattag
     7501 gtaaaatttc tagaagttat agtgataaat atcctgaaaa tgaaattatt aagacaactc
     7561 ctaatacagg tgaacgtgtt gaacgtggtg acagtgttga tgttgttata tcaaaaggcc
     7621 ctgaaaaggt taaaatgcca aatgtcattg gtttacctaa ggagcaagcc ttgcagaaat
     7681 taaaatcgtt aggtcttaaa gatgttaaga ttgaaaaagt atataataat caagcgccaa
     7741 aaggatacat tgcaaatcaa agtgtaaccg caaatactga aatcgctatt catgattcta
     7801 atattaagct atatgaatct ttaggcatta agcaagttta tgtagatgac tttgaacata
     7861 aatcctttag caaagctaaa aaagccttag aagaaaaagg atttaaagtt gaaagtaaag
     7921 aagagtatag tgacgatatt gatgaaggtg atgtgatttc acaatcacct aaaggaaaat
     7981 cggttgatga aggatcaacg atttcatttg ttgtttctaa agggaagaaa gatgattcag
     8041 aagatgtgaa atcaactaca gcatctgttg atgttcctta cactggtaaa aatgataagc
     8101 cacaaaaagt aaaagtttat attaaagata aagataatga cggttcaact gaaaaaggta
     8161 gtttcgacat tacaagtgat caacgtatag acattccttt aagaattgaa aaaggaaaaa
     8221 cagcaagtta tattgttaaa gtagacggta aaactgtagc tgaaaaagaa gtcagctatg
     8281 atgatattta aatataattg aagtaaatgt accgaggttt ctatttggaa gtctcggtat
     8341 ttttatattg agattgcggt agttttaaaa tgcttcatgt catcatatac aggtgatcat
     8401 tgactatgaa ttctgtatca ctcccacaat atttatatca tttgaaatga tcatttatat
     8461 attaattttc cagtctttga tataataaat gcagtaaaat taatcgagag gtgccatttt
     8521 gaagacaggt cgaatagtga aatcaattag tggggtatat caagtagacg ttaatggcga
     8581 acgtttcaat acaaaaccac gaggattatt tagaaagaaa aaattttcac cggtagttgg
     8641 tgatatagtg gaatttgatg tacaaaacat taacgaaggc tatattcatc aagtgtatga
     8701 gcggaaaaat gagttgaaaa gaccacctgt aagtaatata gatacactag taattgtaat
     8761 gagtgctgtc gagccaaatt tttcgacgca attattagat cgatttttag ttattgctca
     8821 ttcgtatcag ttaaatgcga gagttttggt gactaaaaaa gataaaacac caattgaaaa
     8881 gcagctcgaa attaatgagt tgttgaaaat atatgaaaat attggctatg agactgaatt
     8941 tattggaaat gatgatgatc gaaaaaaaat tgtagaagct tggccagcag gacttatagt
     9001 acttagtggt caatcaggtg tcggtaagtc cactttctta aatcattatc gtccagaact
     9061 taatcttgaa acaaatgata tatcaaaatc attaaatcga ggaaagcata ctacaagaca
     9121 tgtcgaacta ttcgaacgtc aaaacggtta tattgcagac acacctggat tcagtgcttt
     9181 agattttgat catatagata aagatgaaat aaaagattat tttcttgaat taaatcgata
     9241 tggtgaaaca tgtaagttta gaaattgtaa tcatatcaaa gaacctaatt gtaatgttaa
     9301 gcatcaatta gagataggga atattgcgca atttagatat gaccattatt tacaactatt
     9361 taatgaaatt tcgaatagaa aggttagata ttaaatgaca aaactatatc catcattatt
     9421 atctgttgat tttttggatt tacaacatga attaaaacga cttgaagaag caggtgtcga
     9481 cggggttcat tttgatgtta tggatggtca atttgtgcct aatatatcta ttggtttacc
     9541 aatattagat gcagtaagaa aaggcacaac attacctata gacgtacatt tgatgattga
     9601 aaatccagaa aagtatattg catcatttgc agaacatggt gccgatatga tttcaattca
     9661 tgtcgaatca acgcctcata tccatcgtgc tattcaaatg attaaacatt tagataaaaa
     9721 agctggtgta gtaattaatc ctggtacacc aatatcacaa attgaaccta ttttagacat
     9781 tgttgattat gtactggtga tgacagttaa cccagggttt ggtggtcaat catttattga
     9841 tcaatgcgta gaaaaaatag cgggtcttaa tgctattaaa atggaacgtc aattaaactt
     9901 tgatattgaa gttgatggag gcgtaaatac cgatacagcg aaagtttgtg ttgaaaatgg
     9961 tgctacaatg ctagtaacag gttcattttt ctttaaacaa gaggattata aaaaagtcac
    10021 acaagcattg aaaggttgag tgtatatgca tataaattta ttatgttctg atcgacactt
    10081 gccgcaagat atttgggcca aaagtaatga aggtaaatgg ggcggcgttg atagaggtgc
    10141 tttgatttta ttgaagcatc aaattatccc ttttttctca gtgggagact ttgattcagt
    10201 cagtaaagaa gaacgccaac ttctaacaga acagttacaa atcaaacctg ttcaagctga
    10261 aaaagctgat acggatttag ctttagcggt tgataaagct gttgcacttg gatttgatag
    10321 tattacaatt tatggtgcaa caggcggacg attagatcac ttttttgggg caattcagtt
    10381 attattgaaa aaagcatatt ataaacatga tgttcatata gaagttatcg atcaacaaaa
    10441 taaaattgaa ttattgccta gtggtcaaca tacagttgaa aaagataaga gttatccgta
    10501 catttcattt ataccgatga ctgatgatgt agaactttct ctagcaggtt ttaaatataa
    10561 tttagctaga caaatgctta atataggttc tactttaact atttcaaatg aaattgagtc
    10621 tttgcaagcg aaagtaactg tacacgatgg gttgattttg caaattagaa gtacagattt
    10681 aaattgagta tatacaaatt tgtgaagatt tttaataaag tgcatgagtt atttaataaa
    10741 gtaacgttta taaatagaat ttatagcatt ataatagatg aatcacgctg taattaatga
    10801 agattatggc gtgatttatt attgaaagaa aaattgtagg tcctagtaca ttgattaatg
    10861 tgccaaaatc gatattgaac ccataagtcc aatttgacag agaatcatta ttaaacgttt
    10921 taaccaaatt atattgatat ttcgaatttc actatgaacc attatgtatt gttttgtaac
    10981 aaaaaaagaa ctaagaggtt gagctcttag ttcttaagta aatgcgtcct ccacataagg
    11041 tcacattttt tatgtgcaat atatttatta aactctagtt actttaccag attttaaagc
    11101 acgtgcagaa acccaaactt ttttaggttt accgtcaact aggattctaa ctttttgaag
    11161 gttagcgttc catctacgtt tagtagagtt taaagcgtgt gaacgtctgt taccagtcga
    11221 agctttacga cctgttacga aacattgttt accc
//
LOCUS       DAHHRR010000088        10460 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.088, whole genome shotgun sequence.
ACCESSION   DAHHRR010000088 DAHHRR010000000
VERSION     DAHHRR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.088"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(245..667)
                     /locus_tag="NBI42_001932"
     CDS             complement(245..667)
                     /locus_tag="NBI42_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCD1778335.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            815..1531
                     /locus_tag="NBI42_001933"
     CDS             815..1531
                     /locus_tag="NBI42_001933"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCD1778336.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDLYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIYFKMQKFNPEYVK
                     LAVMPHNKIDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     EPQAPGQIDVTDLKAQVTLY"
     gene            1614..2153
                     /locus_tag="NBI42_001934"
     CDS             1614..2153
                     /locus_tag="NBI42_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCD1778337.1"
                     /translation="MELQQAIANRRSVKKFKKDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEEDMPKAPRKNRNLITLY"
     gene            complement(2303..2623)
                     /locus_tag="NBI42_001935"
     CDS             complement(2303..2623)
                     /locus_tag="NBI42_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1778338.1"
                     /translation="MQAIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            2767..3123
                     /locus_tag="NBI42_001936"
     CDS             2767..3123
                     /locus_tag="NBI42_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCD1778339.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            3281..3661
                     /gene="gcvH"
                     /locus_tag="NBI42_001937"
     CDS             3281..3661
                     /gene="gcvH"
                     /locus_tag="NBI42_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCD1778340.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQLEALLTAEKYSEMIGE"
     gene            3840..4718
                     /locus_tag="NBI42_001938"
     CDS             3840..4718
                     /locus_tag="NBI42_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HCD1778341.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLSQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLNDLPEQQELFPVNEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLKDIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAREP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            5305..5691
                     /locus_tag="NBI42_001939"
     CDS             5305..5691
                     /locus_tag="NBI42_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCD1778342.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPFLPNINNERLVLVNE"
     gene            5684..5980
                     /locus_tag="NBI42_001940"
     CDS             5684..5980
                     /locus_tag="NBI42_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1778343.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     regulatory      6038..6141
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            6230..7255
                     /locus_tag="NBI42_001941"
     CDS             6230..7255
                     /locus_tag="NBI42_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCD1778344.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKSREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEHGPVTQVFENPQHTVTKRFVKEDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     gene            7248..7943
                     /locus_tag="NBI42_001942"
     CDS             7248..7943
                     /locus_tag="NBI42_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1778345.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            7961..8782
                     /locus_tag="NBI42_001943"
     CDS             7961..8782
                     /locus_tag="NBI42_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1778346.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGSKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            9137..9331
                     /locus_tag="NBI42_001944"
     CDS             9137..9331
                     /locus_tag="NBI42_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCD1778347.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(9565..10416)
                     /locus_tag="NBI42_001945"
     CDS             complement(9565..10416)
                     /locus_tag="NBI42_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCD1778348.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTGLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFTGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT         3786 a         1454 c         1888 g         3332 t
ORIGIN      
        1 ggaaattatg gaattggtta ttggtcgaca ctggactttt atttttagac ggtaagcgaa
       61 gaagtcaagc cagtattgga accggtcaca ttcatgatga ctaagggaca atcattttca
      121 gttaaatatt gccagaaatt tcaatcacga atagaaaaaa taaccaacca aaaatttttg
      181 gttggcaaat ttgaatttta tgtgaatttt attaataacg ttgaataatc tctttaagtt
      241 aatgctaatc tacaacattt acatttaaat cgacattaat atttccttga gtcgcttttg
      301 aatatggaca aaattcatga gccatttgta aatatttttc agcttcttct tgagatataa
      361 catttttaat tgtcgcatca attgaaacac ttaatttagg actttctgag tctgaatcat
      421 cttctagtct cactgttagt gttacttctg gatgagcatc acgcactttg ttttgcttta
      481 aaattaggtc gaaagcaccg ttgaagcaag atgcataacc tgctgcaaat aattgttcgg
      541 ggttagtagc tttaccatct gcttgagcag gcggaactat atcaatatct aacgcgcgat
      601 catcagtata aacatgtcct ttacgtccgc caacattcgt cgctttagtt tcataatgta
      661 ttgccattct tataacctcc taatttaaaa tacatttatg ctttacccat tcgaaaatac
      721 ttaaatcatt ttcatttata gcattgtttg attgaaggat aaaaagttgt tgtttacaat
      781 aaaaataatg agtatctgaa atgagggatt tactatgaca catgtggaag tagtagcgac
      841 tatcgcgcca caattatcta tcgaagaaac tttaattcaa aaaattaatc atcgtattga
      901 tgcaatagac gtattagaat tacgaattga tcaaattgaa aatgtcacag ttgatcaagt
      961 ggcagaaatg attacaaagc tgaaggttat gcaagattca ttcaaattat tagttacgta
     1021 ccgtacaaag ttacaaggtg gctatgggca atttacaaat gacttgtatc ttaatttaat
     1081 atcagactta gcaaatatca atggcataga tatgattgat atagaatggc aagcagatat
     1141 tgacattgaa aaacatcaac gaatcattac acatttgcaa cagtataata aagaggtggt
     1201 tatatcacat cataatttcg aaagtacgcc tccattagat gaattgcaat ttatatattt
     1261 taaaatgcaa aaattcaacc cagaatacgt taaattagca gtaatgccac ataataaaat
     1321 tgatgtgtta aatttattgc aggcaatgtc tacattttca gatactatgg actgcaaagt
     1381 tgttggtatt tcaatgtcta aacttggact aataagtaga acggctcaag gcgtttttgg
     1441 tggtgcgttg acttatggtt gtatcggaga accacaagct ccaggacaga ttgatgttac
     1501 tgatttaaaa gcacaagtga ctttatacta ataaaatgaa ttaggttgct tttgtgtaag
     1561 tgctgtctta taattgagaa gtattatcat tgttcgtgga ggagcgattt tagatggaat
     1621 tacaacaagc aatagctaat agaagaagtg tgaaaaaatt taaaaaagat atgcacatag
     1681 atgacgcatt gctatatcaa gcaattgaga aagctgctga tgctccaaat cacggaatga
     1741 gggaaccatg gagagttgtg catgttccga aagacagatt aggagatatg agtaaggata
     1801 tttctaaatt tgcatttcct aatgaattag ataagcaaca atgtcattat gatgcagtaa
     1861 cgaaactagg tggcatgtta ttgcttattt taaaaacaga tccaagacaa cgccaaaatg
     1921 atgaaaacta ctttgcattt ggtgcatatg cacaaaatct tatgttgtta ctttatgaag
     1981 cgggaatagg tacatgttgg aaatcgccat tatatatcta tgatcctaaa gtaagaaaaa
     2041 cacttggtat aaagaaagat gaagttcttg ctggattctt atatttaacg gatttagaag
     2101 aagatatgcc taaagcacca cgtaaaaata gaaacttaat tacattatat taatatgtac
     2161 aattatagaa acattaataa aagctgagac atgaattgat ggacatctat cgagttagag
     2221 atttaatcta acttactaga gtcgttacaa tcacagtctc agctttttat tgtgcagttt
     2281 atacacattt ttattttagt atttatttaa aagtttctgc taaaaatgat tcaacttgtt
     2341 caggtgactt agcatttgct gaatgaaggt gtgcaatttt gtcgccgttt ttaaatacta
     2401 gcaagctagg gatacccata acttcatttt caacaactac atcttctaat tcatcacgat
     2461 taacagtata ccattggtaa tcattatatt gttctacgat tgggtcaatc cataaatcca
     2521 tagcacgaca gtctgggcac cagcctgcct caaatttaac aattacaggt gtatcgctat
     2581 taattacaga tttaaatgat tcattacttt tgattgcttg cattgtaaca actcctctag
     2641 atagtttaat aatttttatt atagctaaat ttataacata ataaaaaatt ttagcttcaa
     2701 aatgaaagcc ttttgctatg gaaaatgata tatttatttt aaatacataa aggaggttgc
     2761 agtcgtatga ttaaatttta ccaatataag aattgtacaa cttgtaaaaa ggcagcaaag
     2821 tttttagatg aatatggcgt aagttatgaa ccaattgata tcgttcaaca tacacctaca
     2881 ataaatgaat ttaaaacaat aattgcaaat acaggcgtag aaattaataa attgtttaat
     2941 acacacggcg cgaaatatcg tgagcttgat ttgaaaaata aattacaaac tttatcagat
     3001 gatgaaaagt tagagttgtt atcatctgat ggtatgttag taaagcgtcc tctagcagta
     3061 atgggcgata agataacatt agggttcaaa gaagatcaat ataaagagac ttggttagcg
     3121 taagtataat gtaaacgttt actaaatatc tcgatattta gattcattac atgtaaaatg
     3181 aaataagcta tacaattgtt aatttttata aatatagttg aatagcatct agccttatgg
     3241 catcattaat gatgtaaaga ttaattagga ggggattctc ttggcagtac caaatgaatt
     3301 gaaatattca aaagagcatg aatgggttaa agttgaaggt aatgtagcaa caattggaat
     3361 cacagaatac gcacaaagcg agttaggtga tattgttttt gttgaattac cagaaacaga
     3421 tgatgaaatt aatgaagggg atacgtttgg tagcgtagaa tcagttaaaa ctgtatcaga
     3481 attatatgca ccaatctctg gtaaagtagt tgaagtcaac gaagaactag aagatagtcc
     3541 cgaatttgta aatgaatctc catacgaaaa agcatggatg gtaaaagtag aaattagtga
     3601 tgaaagtcag cttgaagctt tattaacagc tgaaaaatat tcagaaatga ttggtgaata
     3661 atcaccgtgt aactccttaa tctaagattg aggagtttat ttttagtctg agtaagtaaa
     3721 taatatgaaa gaaaatttat taggcactat tatttggagt attgctacat tttattattc
     3781 aagaatgatg aaaataatga atttagctat tttaaaaata aaaattgggg gaagttaata
     3841 tgctaaacat tcaagacgtt agtcatcttt ctaaaaagga gcaaaaagca tataaccgtt
     3901 tcgtagaatc tgtagaaaac ggtaatttac cagtactacc atgtattgaa atggatctaa
     3961 aagagatgca agaagaaact ttaagccaga gtaagattgg tggaatgcca tttttaaaat
     4021 cttttaaaga tataccatta gatgaaaata atgtaccaat ggtattgtta gcacagatta
     4081 atttgaatga tcttccagaa caacaagaat tatttcctgt aaatgaaggg atattgcagt
     4141 tttggattag ttcagaagat caaatgtatg gtatgtctga aaatttaaag ggaaacaata
     4201 taaactcaag gcttgtttat ataaaagagc caattacaga tttatcactc aaagatattc
     4261 aagcgcattt gaagtcatta gatgctgata atgaggatat cccgttcagt ggagcatttt
     4321 ctatagaatt tagattgtcg aaacaaacta ttacatgtac tgattataag tacgatgagg
     4381 acgtgcttgc attgtggaat aaagtcaatc catccttcgc gctaaaatcg atgtttggtg
     4441 gatatgatga attaatggaa cctgtgtgta acacatttac tgctagggaa ccatttaatc
     4501 aacttggtgg ttatccatat tttgaccaaa tagatccaag aacgaacgat caagaactga
     4561 aaatgtatga tagagtctta ctgcaaattg attctacaag agatggtaat tcttcgatta
     4621 tatggggtga tttaggtatt gccaatatct tagtaaaatc tactgacctt gaggctatga
     4681 agtttgatga ttacatgtat tcatgggatt gcagctaaga gaatcataaa aatatattcc
     4741 tttaataagt gtagtatcta tagatcgatc tagggagtgg gacagaaatg ataaagaacc
     4801 actagtgatt tataatgtag tggttctttt tcataagcca cagctaatgt gtatttaaaa
     4861 ataggaatac atgagtaaaa ctcatgcata agaaatacta atttctaaag aaaaagtatt
     4921 tctttatgtt ggggccccga caacttgcat tgtctgtaga atttcttttc gaaattctct
     4981 gtgttggggc ccaccccaac ttgcattgtc tgtagaaatt ggaagtccaa tttctctgtg
     5041 ttggggcccc gctataattg aaaagtttgt tataggtgta ttttcttttg gttaactatt
     5101 ggtaatataa cattgtagat tttaggatgt tgatttatgg tttagccttt gtctgtttat
     5161 gtaaaatgtc ccagctagtc aaacattgag tttgtaatta ttatattaag ctagtttaaa
     5221 ttttttgtga tttatgtttt attcatacca attttgtgaa atgatacagt gacagatgtt
     5281 tattgatttt aaagggtgat gatgatggct attgtaaata aagtgataat tgttgaagga
     5341 aaatctgata aaaaaagggt gcaacaggtt attgcagaac cagtcaatat tatttgtact
     5401 catggaacaa tgagtataga taagcttgat gatatgatag aatcactata tgataaacaa
     5461 gtttttgtat tagccgattc tgatgacgaa ggagatcgaa ttagaaattg gtttaaacgt
     5521 tatttgagtg aaagtgaaca tatatttatt gataaaactt actgtcaagt tgcgaattgc
     5581 cccaaacaat atttggcgca tgtactttca aaacatggct ttacttgtaa gaaagaaaca
     5641 cctttcttac cgaatataaa taatgaaagg ttagttttag taaatgaata attcattaga
     5701 catcaaagat gtaactacat tttatgagga agacaaacat ttaatctttg gttatacacc
     5761 aacttgtggt acttgtaagg tttcagaaag aatgttagac attgctaatg aaatattgca
     5821 gttgccatta ttgaaaatag atttaaactt ttatcctcag ttttgtaaag atatgcaaat
     5881 catgtctacg ccgattttat tgttgatgaa taaagataaa gaagtaaaac gaatttatgc
     5941 atttaaatcg gtgactgatt tgttagaaaa tttaaaatag ttattgacga aattttacta
     6001 aggtgttagg attaatacaa attaaatgat gcgtaaactc ttatcgagag tggtggaggg
     6061 atgtgcccta cgaagcccgg caaccgtctt atatagaaat ggtgccaatt cacataaagt
     6121 tttaactttt gaagatgaga gaaacaatac tactattgct ttctcaattt ttctatcgat
     6181 attgagaaag cattttttat tttattaagc aacacaggga ggaatcaacg tgattgaatt
     6241 aaaagaagtt gttaaagaat atcggactaa aaataaagaa gtccttgctg tagatcacgt
     6301 taatttatcg attcgagcag gatcgattta tggcgtcatt ggtttttctg gagcaggaaa
     6361 aagtactttg attcgaatgt ttaatcattt agaagcgcct acatcaggtg aggttattat
     6421 agatggagac catataggcc aattgtccaa aaatggatta agagcaaaaa gacaaaaagt
     6481 aagtatgatc ttccaacatt ttaatttgtt atggtcaagg actgtgttaa aaaatattat
     6541 gtttccgctt gaaatagcag gtgtccctag aaggagagca aagcaaaaag cattagaact
     6601 tgtcgaactc gtcggtttaa aaagtagaga aaaggcttat ccatcagagt tatcaggtgg
     6661 acaaaagcaa cgtgttggga ttgcacgagc gttagctaat gatccaacgg tcttgctttg
     6721 tgatgaggca acaagtgcac ttgatccgca aacaacagat gaaattttag atctactact
     6781 aaaaattaga gaacaacaaa atttaacaat tgtactaatt acgcatgaaa tgcatgtcat
     6841 tcgtcgtatt tgtgatgaag ttgcagttat ggaaagtggt aaagtgatag aacatggacc
     6901 ggtgacacag gtttttgaaa atccgcaaca cactgtgaca aaacgatttg tgaaagaaga
     6961 tttaaatgat gatttcgaaa catctttaac agaattagag ccattagaaa aagatgctta
     7021 tatcgttaga ttagttttcg ctggttcaac aacaaccgag cctattgtat cgagtctatc
     7081 aactgcctat gatattaaaa ttaatatttt agaagcaaat attaaaaata cgaaaaatgg
     7141 aacagtcggc tttttagttc tgcatattcc atatatttca agtgtagatt tcggaaaatt
     7201 cgaaaaagag ttaattgagc gacaagttaa aatggaggtg ttaagacatg ggtaaatcat
     7261 ttagtgaaat tataaatgaa atgattacaa tgcctaatat tcagtggcca gaagtttgga
     7321 ctgcaatagt cgaaacacta tacatgacag tcgtctcaac tatatttgca tttatactcg
     7381 gtcttatttt aggtgtgtta ttattcttgt ctgctaaagg taagtctatc ggtgcaaggt
     7441 tattttattc tatcgtttct ttcattgtta acttatttag agcgatacca tttattattt
     7501 taattttatt attaattcca tttacaagtt tgatacttgg aacgataagt ggtccgacag
     7561 gtgcgttacc agccttgatc attggcgcag cgccgtttta tgcaaggctc gtagaaattg
     7621 cttttaaaga aattgataaa ggtgtcatcg aagcggcctg gtcaatgggt gctaatactt
     7681 ggacagtaat tcgtaaagtc ctcttacctg aagccatgcc agcgctagtg tctggcatta
     7741 cagttacagc aatcgcttta gttggttcaa cggcagttgc aggtgtaatt ggtgccggtg
     7801 gtttaggaaa tttagcatac ttaacaggtt tcactcgaaa tcaaaatgat gtcattttag
     7861 tatcaacagt ttttattcta attattgtat ttataatcca attcattggg gattggctta
     7921 caaataaact tgataaacga taaattgggg gtttcatttt atgaaaaaat tatttggtct
     7981 tattttagta ttaacatttg cagttgtatt agcagcttgc ggtaatggaa gcaaaagtgg
     8041 cagtgacgac aagaaaataa cagtaggtgc ttcaccagca ccacatgctg aaattttaga
     8101 aaaagcaaaa ccattattag agaaaaaagg ttacgaacta gatattaaaa caattaacga
     8161 ttacactacg cctaataaat tactagacaa aggtgaaatt gacgcaaact atttccaaca
     8221 tacaccatat ttaaacacag agaaaaagga taaaggttac aaaatcgtaa gtgcaggtga
     8281 tgttcactta gaaccgatgg ctgtatactc taaaaagtat aaaagtttaa aagaattacc
     8341 aaaaggtgca acagtttatg tgtctaataa tccagctgaa caaggacgct tcttaaaatt
     8401 cttcgttgat gcaggtttaa ttaagatcaa aaaaggcgta aaaattgaag atgctaagtt
     8461 tagtgatatt acagagaata aaaaagatat taagtttaat aataaacaat cagcagaatt
     8521 cttacctaaa atttatcaaa atgaagacgc tgatgctgtt attattaatt caaactttgc
     8581 aatcgaacaa aaactaaatc ctaaaaaaga ttctattgct gtagaaagtg cgaaagataa
     8641 tccttatgca aacttaattg ctgttaaaga aggacatcaa gatgataaga aaatcaaagc
     8701 attaattgaa gtattacaat ctaaagatat tcaagacttc attaatgaaa aatacaacgg
     8761 tgcagttatt cctgctaaat aaatcatatt aatggctcag taacgtggtg ttgctgggct
     8821 ttttaattta aacaggtatt tatatgaaat ttaggaatga gatgctatta cgtataaagt
     8881 aaaatcctat atgcatgcgt gctactcatt tattcttatt tttaatttca catggatagt
     8941 gattactaca taataaatca ttagtgattc tttattattt cagtccccac tccctcaagt
     9001 taatgatgaa tctatttcgc tagttataaa atgacgtcaa atcaatatca aaaattattt
     9061 aagtaaaatg tttagataat ttttcagtgg gtaagtatta tatataacat ttaattattc
     9121 cgaggaggca tttattatgg cagacgaaag taaatttgaa caagcaaaag gtaatgttaa
     9181 agaaacagta ggtaatgtta ctgataataa aaatttagaa aacgaaggta aagaagataa
     9241 agcttctggt aaagcgaaag aattcgttga aaatgcaaaa gaaaaagcaa ctgattttat
     9301 tgataaagta aaaggtaaca aaggcgagta attttacttt ttattcctaa tacactgcac
     9361 atgccactta gtcggtataa ccatcggcta ggtggttttt tgtattaaaa aagtggatac
     9421 caaaatttat taataattat tttaatgtta gaaaaaaact aaataaaaac tcgctaatga
     9481 tatccaataa tatgtataca aaacgagaca tatattgcat atgattaacg agatactgaa
     9541 tatattttat caaccctaaa atgattattc attttcagcg gtaattcgac ctaaagtcaa
     9601 acttacaata aaaccgatga taaatactac taatgaaacg aaccacatca cgatattagt
     9661 aggtaaacct ggaaatactg caaagaggga gccaacaaca aaaccaatga ttaatgcaaa
     9721 agtcattagt ttatgatgtg ttaggaaata ctggataatt ttgcttgaaa taatgaatcc
     9781 agcaagcacg ccaaatccga ctgcaagtaa tataggaaga ccggtaaagt taagtttaac
     9841 aacttcagat atggctagca tgactgtgcc atagacgcca aatactaata acataaatga
     9901 ccctgaaata cctgggagta acatagcact tgatgcacac atacctgcaa taaagtattt
     9961 aataataaga ccagttgata gagtaagtgt ttctccagca tgtttatcgc cattattcat
    10021 taatgtaata acaattaaga tagcgatacc agctataacc atcatgtaat gtttagttgt
    10081 aaatgacgtt ttatagttag aaattttcaa taaatatgga acgataccaa tgattaatcc
    10141 accaaagaaa aacatagttg gaatatggtg ttggcttaat aaataattaa aaagattact
    10201 tagtgatccc attgccagta acattccaat tataatgggg attaaaaatg taaaacttgg
    10261 ccaaaaacgt cgtgagaata tgccgctaat tgaagcgata aattgattgt atatacctaa
    10321 caataatgcg atagtcccac cgctaacacc aggtaccaag tcactcgttc ccatagcaaa
    10381 accttttaga atattaatcc atttaaattg ttgcatgaat aactcctttc aaacgattgg
    10441 aataaaatca taaatagcat
//
LOCUS       DAHHRR010000089        10292 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.089, whole genome shotgun sequence.
ACCESSION   DAHHRR010000089 DAHHRR010000000
VERSION     DAHHRR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.089"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..125)
                     /locus_tag="NBI42_001946"
     CDS             complement(<1..125)
                     /locus_tag="NBI42_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCD1778349.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLN"
     gene            264..554
                     /locus_tag="NBI42_001947"
     CDS             264..554
                     /locus_tag="NBI42_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778350.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            554..2341
                     /locus_tag="NBI42_001948"
     CDS             554..2341
                     /locus_tag="NBI42_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCD1778351.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            2575..3924
                     /locus_tag="NBI42_001949"
     CDS             2575..3924
                     /locus_tag="NBI42_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1778352.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            3946..5574
                     /locus_tag="NBI42_001950"
     CDS             3946..5574
                     /locus_tag="NBI42_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1778353.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            6096..6446
                     /locus_tag="NBI42_001951"
     CDS             6096..6446
                     /locus_tag="NBI42_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778354.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            6533..6844
                     /locus_tag="NBI42_001952"
     CDS             6533..6844
                     /locus_tag="NBI42_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCD1778355.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            6859..7365
                     /locus_tag="NBI42_001953"
     CDS             6859..7365
                     /locus_tag="NBI42_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCD1778356.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            7417..9024
                     /locus_tag="NBI42_001954"
                     /pseudo
     CDS             7417..9024
                     /locus_tag="NBI42_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(9015..9275)
                     /locus_tag="NBI42_001955"
     CDS             complement(9015..9275)
                     /locus_tag="NBI42_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCD1778357.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(9247..10233)
                     /locus_tag="NBI42_001956"
     CDS             complement(9247..10233)
                     /locus_tag="NBI42_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCD1778358.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
BASE COUNT         3861 a         1768 c         1621 g         3042 t
ORIGIN      
        1 tcatttaaat cgcttaaact tcttaataac aacaaatctt cataaacctc aactgttaca
       61 tcaaatttca aataatcaac taagtttcca tacttatcaa taatttcttc tagcgtaggt
      121 atcaatatat accccccctc cccatttttt gttccgcgtc taatcataat gcttcattta
      181 tgcttattat agcaatcaat tgaagatagt ttaacctata tgatattgat tgactcctta
      241 aaaattattg gaggaatttt aaaatgaata agaaatctaa gcagcaagaa aagctttata
      301 attttataat tgctaaatct tttcaacaac cagttggaag tacgttcact tatggtgaat
      361 taagaaagaa atataatgtg gtttgtagca cgaatgatca acgtgaagta ggacgtcgtt
      421 ttgcttactg gattaagtac acgccaggat taccatttaa aattgtagga acaaaaaatg
      481 gcagtttatt atatcagaaa atagggataa acccatgcaa taatagcacg ccttcgaaag
      541 gaggtgattg ctaatgagtt acacattgtt tgaaattggc ccatttgctt tagattcctt
      601 cggttggaaa gaaacgatac cacctaaaaa agatggtgac agtgaaaaag tggttagaat
      661 ggctagccct attattgtta atgcaagatt ttctgatcca attactggtg ttgaaaagtt
      721 gattattact aacaacaatg gtaaaaaaga catttttgag tctgatatct taacaacacg
      781 aaacttacct tctttaatta aatatggcta cagtattaac gagaagtata ttagatcatt
      841 gagttatgcc ttgcaattga tgcgagatag attaccactt tctgaattat atgagggtgt
      901 aggtatacta gagacacctt ttggctactt gatatcgcta gataaagttc tcaaatctat
      961 tcaatttaat cagtcctcac cttcataccc tattgtagat agtgcttatg acttaactcc
     1021 taaaggtaca tttgataatt ggtttaacat gtatattgac gaggttaaag gtcatcttct
     1081 tttagaattg gcagtcatat ttgggatttc tgccttagtg acaagttttc tgaagcataa
     1141 acatgaaatc gaatttgcag gaattctctt tagttttaca ggtcaatcct caaccggtaa
     1201 gtcaaccgca gctgctttag ctgtatcagt cgctggtaac cctaccaaag gtaatgaaac
     1261 tctttttaga agttggaatg ccacacgaaa cgcacttgaa ggttatttga gtaacaatta
     1321 tggcattcct atagtatttg atgagctttc atctacgaca cttagagata ccacgggttt
     1381 gttatattcc atcgctgaag gtcaaggtcg tcaacgttca aacgttcatg gagaagttaa
     1441 aacgcctaag aattggggta cttctgtcat ttcaacgtca gaatacagta tttttaatga
     1501 ttcagctcaa aacgatggat tacgtgtaag aaccatcgag attaatgaac aattcactac
     1561 aaatgcgacc aatgctgaca atattaaaaa agctgtagca ctaaactatg gtcatgtttt
     1621 accgttagta gctaaatatc ttataaatcg tgaagatgaa gttattcaat ggttttacaa
     1681 ggaagtagat tggtttgaag ctaaattgaa agatgaaaca aacaatacag gtattcgtat
     1741 gtttaagcgt tatgcagtca ttacaacatc tgcgaaaata ttaggtcgtg ttttatctac
     1801 agatattgat atcgctaata taagagacta ctttattgat tatcatactc atacagtctc
     1861 tgaacgctca ttagctgata aagctattga tgtcatcatt caatttgtgg cacaaaacag
     1921 aggtaagttt tcagatgaag gtgctttgaa aaatatgttt gagaactatg gattaatctc
     1981 attaaaagat gaccatatcg aagtaaaaat aattgcgaac gtttttaaac agatgctaaa
     2041 caatcatcaa tttcaagatg ttaataatgt tgtcaacgcc ttaagagata aaggtttcat
     2101 attagctgac cgtggacgcc aaacaactaa aagaagtgtc aaagataata gcggtaaaaa
     2161 acaatcactt gtattttacc atctaaaatt agatgtagag tttgcatcta tcctcggatt
     2221 aactaaagat aagtcgttac ttcaaaattg gacaccgtca aatgataaca aagctgcgaa
     2281 agaacttttt aaatccgcta atgaagggat aggcccttca ggagttcacg aagatttcta
     2341 attgtcttta tcatacaact gtgaagcatc aaactgaata atcgacgtat tgaagataga
     2401 acaaataacg gagtgaaaaa catggatgaa cttatcgaga tattagccga tttggtaatt
     2461 gaagaaatca atcaaacaaa tgactaatat ggacgcccaa catctcattg atgttgggct
     2521 tatatatcaa ttcatccata aatatatatt atcactgtga aaggaggagc cgatatgaaa
     2581 caagtcatag gctatttacg tcaaagtacg atgaaacaac aatctcttgc agcacagaaa
     2641 caagctatcg aagcaatagc cgaaaaacat catattcaac atatcaactt ttatagcgac
     2701 aaacaatcag gtcgcaaaga taatcgtagt gggtatcgac aaataacaca attaattcaa
     2761 caaggacagt gtgacatatt atgctgttat cgtcttaata ggttgcatcg taatctgaaa
     2821 aatgcattaa aactcatcaa attatgtcaa acgtaccatg ttcatatctt aagcgtacat
     2881 gatggttact ttgatatgga tcaagctttc gaccgactca agcttaatat cttcatcagt
     2941 ttagccgaac ttgaatcgga taacattgga gaacaagtca gaaatgggct tcaagaaaaa
     3001 gcaaagcaag gtcgattgat tacaacacat gcaccttttg gttacgaata tcacaacgga
     3061 acattcatca tcaatcaaaa tgagtcacca acggtaaagg ctgtattcaa ttattacatt
     3121 aaaggtcatg gttataagaa aattgcacag ttattagaag aagataacac gtatatcaat
     3181 cgacaaccct atcaagttcg taacattatc atcaatccta attattgtgg tcgtgtcaac
     3241 aatcaatatg gccaattcga caatatgttt ccttctattg tttccacaag tatatatgag
     3301 caagcgcaga gacttcgatt gcaaaaacaa accaaacaga caccttcgga taatcaactc
     3361 aaacaaaaaa tcaaatgccc atgttgtaat gcaacactta caaatatgac cattagaaaa
     3421 aagaatcata cattacgtta ctacgtctgt cctaaaaaca tgaatgcttc acgctttgtc
     3481 tgtgatttta aaggcatcaa tgcacaaaca cttgaagata aagtattaga agtgtgccga
     3541 gacttttatc aaaatcaacg catctacaca aaaattaaaa gtgcgattga caaacgcatc
     3601 aaaagacaaa gaaacataga aaaacatcac acattgactc aagaacaact gatagaaaag
     3661 ttggcacaag gcatcattga tgcagaaacg ttcagagagc aaacacaatc attacgtcaa
     3721 caaccgcaac gcactacatc tatcaatggg catcaaatac aacacatcat tcaaaatatt
     3781 attcaaaaac gtttcacgtt aaacatattg tacccctata ttgaaaccat tcacattacg
     3841 aaagataaaa atcttatagg aatctatttc aaaaatgaac cactcaatat tgtcaatcaa
     3901 accatgcaat catcgattgc ataaatgaag aaaggatgta acataatgca acaacttaaa
     3961 acaaaacgtg tcggtatcta tgtacgtgta tcaacagaaa tgcaaagcac agaaggttat
     4021 agtatcgacg gacaaatcaa tcaaatcaaa gaatactgtg acttccatca ttttgaagtt
     4081 aaagatatat acgctgaccg tggtatttca ggtaaatcta tgaacagacc tgagctccaa
     4141 cgtatgttga aagatgcaaa agaaggcaat atcgactgtg ttatggtata caaaacaaac
     4201 cgattagctc gtaatacatc tgatcttctc aaaattgtcg aagatttaca caaacaaaat
     4261 gtcgaatttt tcagtttgtc agagcgtatg gaagtcaata cttcttctgg taaactcatg
     4321 ttacagatac ttgcgagttt ctcagaattc gaacgtaata acattgtcga gaacgtattt
     4381 atgggtcaaa cgagacgtgc ccaagaaggc tattatcaag gcaatttacc actaggttat
     4441 gacaaaatac cagatagtaa acacgagcta atgattaacc aacatgaagc taatattgta
     4501 aaatatatat tcgagtgcta tgccaaagga catggctatc gtaaaattgc caatgcattg
     4561 aatcacaaag gatatgtcac taaaaagggg aaacctttca gtattagttc aatcacatac
     4621 atcttagcta accctttcta tatcggcaaa attcaatttg cgaaatacaa agattggagt
     4681 gaaaaacgtc gtaaagggct gaatgataaa ccagtgatag ctgaaggtaa gcattccccc
     4741 attattaatc aagatttatg ggataaagta caaatgcgta agaaacaagt cagtcaaaaa
     4801 ccccaagtcc atggcaaagg aacgaatctg cttacaggca ttattcactg tccccaatgt
     4861 ggcgcaccta tggcagcaag caataccacg aatactctta aagacgggac taagaaacgt
     4921 attcgttact attcatgtag taattttcgg aacaagggtt ccaaagtatg ttcggcaaac
     4981 agtgtaagag ctgatgtgat tgaagattat gtgatgaagc aaatacttga aatagtcaaa
     5041 agtgataaag tcattcaacg cgtagtaaca cacgtcaatc aagaaaatca agttgatggc
     5101 gctgcacttc atcacgatat tgcttataag caacaacaat atgatgaagt acaaatcaaa
     5161 ataaataact tgattaaaac catcgaggat aatccggact taacatcagt aatcagacca
     5221 agtattcaaa aatatgaaaa gcaactcaat gacattacga atcaaatcaa ccaactcaaa
     5281 aatcaacaaa atgaagataa gactttattt gatgccaaag aaatcagtaa actattacaa
     5341 cacatctttc atgatattaa gcacatagaa aaatctcgac tcaaagcatt gtatctatca
     5401 gtgattgatc gcattgatat taaaaaagat ggtaatcata aaaaacaatt ctatgtcaca
     5461 ctcaaactta ataacgaaat cattaaacaa cttttcaata ataaacaact cgacgaagtg
     5521 catctcagca cttcgtcttt atttttgccc caaacactat atcttactat ttagctaata
     5581 gaatcattgt tataacaata attttcacta tattctttcg cttattttat ctaactattt
     5641 tcaagaattg ctctttatca atttcaataa cacttatcta atgaaacagt tattgtcgta
     5701 attttttcat aactatacaa tgcttatatt gaaattgcta aagaacatcc cacaatgatt
     5761 ttttatcggg aaatgattgg gatttgcaga ttcgaagaaa cagaaggatt gtgcaagcac
     5821 aatcccaaaa tcccgttact ttttataaat aaataatata tataaggaag cagagacaca
     5881 accctatcat caatacttat aaataatgag ttgattgttt gtcaaaatag gcggaatttc
     5941 aggatttcct tggcatacca acgtttgtag cgggttttta tatggaattt tctcttattc
     6001 ccaccgggat tcgtaataaa tgacaaatca aatatatatt atctcaatga tgtatatgaa
     6061 ctacatcact attacactat ctaggaggca ataatatgaa aaccatcact caagaactca
     6121 aacaatatat aacccaccta ttccaactat ctaataacga agcttgggaa tgtgaagcgc
     6181 tggaggaagc agcagaaaat attctaccgg aacgttttat taatgattct ccactggtac
     6241 atctcacact cgagacttat acctactata atgatgaact acatgagcta agtatctatc
     6301 catttctaat gtatgccaat aaccaactca tcagtgtcgg atacttagac cattttgaca
     6361 tggactttct gtaccttaca gatactcaaa atatcattat tgatgaacgt catttactaa
     6421 aacaaggagg tcaggaccat gaataattgg atcatagttg ctcaattatc cgtcacagtt
     6481 atcaacgaaa tcatcgacat catgaaagaa aagcagaagg gagggaaata gcatgaaaat
     6541 caatcgatac atcacaagag gaattaatga aagtattcca ctagaccttc aaatcttact
     6601 ttggcacatg gtagaaaaaa aagataacca gcctcataca gattacctac acatttttaa
     6661 attacaagaa gatgagaata tactctcaat cacacatgaa caagaacaac ccacatacaa
     6721 attggaatat cactatacaa actatgaaaa aaatcaaaat gcattaccta agaaagtcta
     6781 cgtcattcgt gaagatgacg tggacgcttt ttattatgtg atgcttttac cagaagaata
     6841 ctaaaggaag tgacgatgat gaatacaatc aaaagtacga tacacacaga agccatattt
     6901 agcgatgatg aacagcaccg ctatttactc aagaaaacat gggatgaaaa gaaagctgca
     6961 tgcacagtga taacgatgta tcctcattta gacggtgtat tatcactcga tctcacaact
     7021 gttcttatcc tcaaccaatt agccaattca gaacgatacg gtgctgtata tcttgtaaat
     7081 ctattctcta atattagaac acccgaaaac ctcaaacata tcaaaaatcc atacgatgag
     7141 cacactgata ttcatttgat gaaagcgatt agtgaaagtg acacagtgat tcttgcttat
     7201 ggtgcctatg cgaagcgacc agttgttatc gaccgtgtcg aacaagtgat ggaaatgtta
     7261 aaacctcata aaaagaaagt aaaaaagctc atcaatccag taacaaatga aattatgcat
     7321 ccactcaacc ctaaggcacg tcaaaaatgg attttgaaat catagcaaat acatggaggt
     7381 caatatggaa acaaaacaaa tcaacatcgt tttactgtaa atgatattgt tgaaacaatt
     7441 cctgacaatg aattcgacga attcagacat caccgtggtg caacttcgta ccatcctaaa
     7501 atgatgttaa aagtgattct atatgcctac acacaatctg tattctcagg tcgtaaaata
     7561 gaaaaaatgc ttaatgatag catccgaatg atgtggctat cacaaaatca aaaaccttct
     7621 tataaaacaa ttaatcaatt tagagtaaat ccaaaagtag atgctttatt agaatcttta
     7681 tttattcaat tttacagtca gtgtgtaaaa caaaatctta tagatgataa agctattttt
     7741 attgatggta caaaaattga agcaaatgcc aatcgatata catttgtatg gaaaaagagt
     7801 attcaaaacc atgaatcaaa gatgaatgag gattctaaag ccctctacca tgaattggta
     7861 accaataaaa tcataccgga aattaaagaa gatcatgata atgaattaac aaaagaagaa
     7921 atagatttga ttggtagtca cttagataaa gaaatcgaag atttaaacca acatatcaac
     7981 aatgaaaaat gtactaaaac aagaaaacaa atacgtctca aaagaactaa aatcaaaaaa
     8041 tacaaaaagc aaatcaatga ttattttgag cgaaagtatc gatacgaatt tcaaaaatct
     8101 attttaaagg atagaaatag ttattctaag acagattatg atgcgacatt tatgagaatg
     8161 aaagaagatc acatgaaaaa tggacaactt aagccagggt ataatttaca aatagcaaca
     8221 aattcccaat ttgttttatc ttataatgtg tatcaaaatc caacggatac tagaacgatg
     8281 attccatttt taaattcaat tcaagagacc tacggtcatt tacctgaata tattgtagct
     8341 gatgcaggtt atggtagtga atcaaattat aaggcaatta tagatgactt taatcgaacg
     8401 ccactcataa catatggaat gtttataaaa gataaaacta aaaaatataa aagtgacatc
     8461 tttaatactc aaaattggaa ctatgacgaa attaatgacg aattcatttg tccgaataat
     8521 aaacggctag gttttaaaag atatgcctat cgtcatgata agtatggtta taagcgagac
     8581 ttcaaattat atgaatgtga tgattgttca gaatgtcctc tgaaaaatca atgtatgaac
     8641 ttcaattcaa aaacaaacaa aaaaataatg aagaattata actgggaata ttttaaatcc
     8701 caaattaaca aaaagctttc agaaccagaa acaaaaaata tccacagtca aagaaaaatt
     8761 gatgtggaac ctgtttttgg atttatgaag gctattttgg gtttcactcg gatgtctgtc
     8821 cgaggactca ataaagtcaa aagagaactt ggttttgtat taatggcact taatataaga
     8881 aaagtagtag ctcaacgagc tgaaaataat caaaaaattt ataaaaaaga caatttctat
     8941 attatttcaa tagaaattgt cttttttcac ttatccaaga actttatgtc ccggactcat
     9001 tatgatttat ttttttattg ctgacttgcc attaattctg cttgctctac aacttgttcc
     9061 acagccattt tttgaagatc tggtggatag ccatatttct ttaataggcg tctaacttga
     9121 actctcattt tagctttagc actgtctcgt ttagaccaat caacactcat gttatcctta
     9181 actgttttag ttaactcatg tgcaatggct cttaactctt tatcgcccaa agcttcttta
     9241 gctgtttcat gtgaagctcg atatactaca tacaatatac gcaacaatac gcttgtttcg
     9301 attaggttta ggcattgaat cataaagtgc aatatactta acacgttctt taatatttga
     9361 attaaaacct agtaaatatt gtgctgccac attatttatg tgctgagatt ttaaaataga
     9421 gcattttaat atcgattcac cataacgtat atgttcatgg cgattcaaaa tttttaaaac
     9481 gtttctatca catacttttt cacagtcatt gtccatcatt gttttactta tatatagtgc
     9541 aggattaaac cagaatatca ttttaaaaac aacataaagc tggttgaata ttaagtcatg
     9601 acttttcaca tgtgatagtt catgtagaat aatatattca atttctttgt cattcatggt
     9661 ttcgactacg acagttggta gtacaatttg ggatttcact aaaccaaata ccatcggatt
     9721 atcaatgttt gaactataac taattgttat atgctttttg tagaactgca tcttactttg
     9781 acatacttta agtcgttcat taagatatga cgattccaat gacgaacttt taataacatc
     9841 aatttgtcgg aatgccttaa tcatataaaa taagcacaac aaactaccaa atacccatat
     9901 caaaagaatc atatacgtta tatttgaggt ctcaaactga ttaacattaa ttgctaagtc
     9961 tttcgtaaca gatgattgtt gaccatctaa catatgacta accgaagaag tcgtgtcaga
    10021 tacatttcga ttcatcatat cttttgaaaa tgtaaaattc gatattttgt aaaatggtat
    10081 taatggaatt aacgtggaga cgagcactaa taaccaaatc ttatgtgaca taatattttg
    10141 agtatatttt atatagagca ttctcactaa aaaaattaca catatcgtga gcaatgaact
    10201 gattatactt aacattaaaa aagatgataa caccttctac acctccatat cacaaaaatt
    10261 ataacattat tttgacataa atactacatt tg
//
LOCUS       DAHHRR010000090        10237 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.090, whole genome shotgun sequence.
ACCESSION   DAHHRR010000090 DAHHRR010000000
VERSION     DAHHRR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10237
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.090"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            582..980
                     /gene="nrdI"
                     /locus_tag="NBI42_001957"
     CDS             582..980
                     /gene="nrdI"
                     /locus_tag="NBI42_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCD1778359.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            943..3048
                     /gene="nrdE"
                     /locus_tag="NBI42_001958"
     CDS             943..3048
                     /gene="nrdE"
                     /locus_tag="NBI42_001958"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCD1778360.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            3166..4137
                     /gene="nrdF"
                     /locus_tag="NBI42_001959"
     CDS             3166..4137
                     /gene="nrdF"
                     /locus_tag="NBI42_001959"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCD1778361.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            4697..4852
                     /locus_tag="NBI42_001960"
     CDS             4697..4852
                     /locus_tag="NBI42_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778362.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLGSKNAT
                     INEKYYQ"
     gene            4891..5862
                     /locus_tag="NBI42_001961"
     CDS             4891..5862
                     /locus_tag="NBI42_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1778363.1"
                     /translation="MKYLLKGNILLLLLMLLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            5849..6805
                     /locus_tag="NBI42_001962"
     CDS             5849..6805
                     /locus_tag="NBI42_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCD1778364.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDIEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            6802..7563
                     /locus_tag="NBI42_001963"
     CDS             6802..7563
                     /locus_tag="NBI42_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1778365.1"
                     /translation="MIQVGNLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFDEGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMDIQIEHIRNQRICLYFKD"
     gene            7682..8710
                     /locus_tag="NBI42_001964"
     CDS             7682..8710
                     /locus_tag="NBI42_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1778366.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKETVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEVKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAVSGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(8971..9285)
                     /locus_tag="NBI42_001965"
     CDS             complement(8971..9285)
                     /locus_tag="NBI42_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778367.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(9303..10226)
                     /locus_tag="NBI42_001966"
     CDS             complement(9303..10226)
                     /locus_tag="NBI42_001966"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCD1778368.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKLTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
BASE COUNT         3598 a         1461 c         1818 g         3360 t
ORIGIN      
        1 tcttgatttc tgttgataca attgagattg gcacagattt aaaaaaatca agtgatatct
       61 actaaaaaaa atttttaaat ttgttcaagt ttttctaatt tagtattggt gcctagttgg
      121 aacgttttac gaacattcga ttagaaaatg gcactttaaa tcatagtgtg tcttatgtat
      181 aatgaaacac ataatatagt gttggtgaaa cgaaaaagac acaatatctt gtgttttgta
      241 tgcaaatgct ttatttatga agaaattaca tttaaaagta atttaacaca gaaatttaat
      301 agttattatc aattaatagt catattttta gaaaatgtac tgagcaaatg gaagatatcc
      361 aatgatgtaa acactacata tagtgatttt tatacattca acccatataa gctactattt
      421 tctcaaatat aaatctatgc aattggttta catttgagaa aataagtagc ttcattatag
      481 ttaatacaat gctgagataa ccatagtaac catgttgtta aagcattttt taattgtaat
      541 gactacttta tttaaaaggg ttgaagaaag aaggtgatcc aatgaaaata atatattttt
      601 catttactgg aaatgtccgt cgttttatta agagaacaga acttgaaaat acgcttgaga
      661 ttacagcaga aaattgtatg gaaccagttc atgaaccgtt tattatcgtt actggcacta
      721 ttggatttgg agaagtacca gaacccgttc aatctttttt agaagttaat catcaataca
      781 tcagaggtgt ggcagctagc ggtaatcgaa attggggact aaatttcgca aaagcgggtc
      841 gcacgatatc agaagagtat aatgtccctt tattaatgaa gtttgagtta catggaaaaa
      901 acaaagacgt tattgaattt aagaacaagg tgggtaattt taatgaaaac catggaagag
      961 aaaaagtaca atcatattga attaaataat gaggtcacta aacgaagaga agatggattc
     1021 tttagtttag aaaaagacca agaagcttta gtagcttatt tagaagaagt aaaagacaaa
     1081 acaatcttct tcgacactga aatcgagcgt ttacgttatt tagtagacaa cgatttttat
     1141 ttcaatgtgt ttgatattta tagtgaagcg gatctaattg aaatcactga ttatgcaaaa
     1201 tcaatcccgt ttaattttgc aagttatatg tcagctagta aatttttcaa agattacgct
     1261 ttgaaaacaa atgataaaag tcaatactta gaagactata atcaacacgt tgccattgtt
     1321 gctttatacc tagcaaatgg taataaagca caagctaaac aatttatttc tgctatggtt
     1381 gaacaaagat atcaaccagc gacaccaaca tttttaaacg caggccgtgc gcgtcgtggt
     1441 gagctagtgt catgtttctt attagaagtg gatgacagct taaattcaat taactttatt
     1501 gattcaactg caaaacaatt aagtaaaatt gggggcggcg ttgcaattaa cttatctaaa
     1561 ttgcgtgcac gtggtgaagc aattaaagga attaaaggcg tagcgaaagg cgttttacct
     1621 attgctaagt cacttgaagg tggctttagc tatgcagatc aacttggtca acgccccggt
     1681 gctggtgcag tgtacttaaa tatcttccat tatgatgtag aagaattttt agatactaaa
     1741 aaagtaaatg cggatgaaga tttacgttta tctacaatat caactggttt aattgttcca
     1801 tctaaattct tcgatttagc taaagaaggt aaggactttt atatgtttgc acctcataca
     1861 gttaaagaag aatatggtgt gacattagac gatatcgatt tagaaaaata ttatgatgac
     1921 atggttgcaa acccaaatgt tgagaaaaag aaaaagaatg cgcgtgaaat gttgaattta
     1981 attgcgcaaa cacaattaca atcaggttat ccatatttaa tgtttaaaga taatgctaac
     2041 agagtgcatc cgaattcaaa cattggacaa attaaaatga gtaacttatg tacggaaatt
     2101 ttccaactac aagaaacttc aattattaat gactatggta ttgaagacga aattaaacgt
     2161 gatatttctt gtaacttggg ctcattaaat attgttaatg tgatggaaag cggaaaattc
     2221 agagattcag ttcactctgg tatggacgcg ttaactgttg tgagtgatgt agcgaatatt
     2281 caaaatgcac caggagttag aaaagctaac agtgaattac attcagttgg tcttggtgtc
     2341 atgaatttac acggttacct tgcaaaaaat aaaattggtt atgagtcaga agaagcaaaa
     2401 gattttgcaa atatcttctt tatgatgatg aatttctact caatcgaacg ttcaatggaa
     2461 atcgctaaag agcgtggtat caaatatcaa gactttgaaa agtctgatta tgctaatggt
     2521 aaatatttcg agttctatac aactcaagaa tttgaacctc aattcgaaaa agtacgtgaa
     2581 ttattcgacg gtatggctat tcctacttct gaggattgga agaaactaca acaagatgtt
     2641 gaacaatatg gtttatatca tgcatataga ttagcaattg ctccaacaca aagtatttct
     2701 tatgttcaaa atgctacaag ttctgtaatg ccaatcgttg accaaattga acgtcgtact
     2761 tatggtaatg cggaaacatt ttaccctatg ccattcttat caccacaaac aatgtggtac
     2821 tacaaatcag cattcaatac tgatcagatg aaattaatcg atttaatagc gacaattcaa
     2881 acgcatattg accaaggtat ctcaacgata ctttatgtta attctgaaat ttctacacgt
     2941 gagttagcaa gattatatgt atatgcgcac tataaaggat taaaatcact ttactatact
     3001 agaaataaat tattaagtgt agaagaatgt acaagttgtt ctatctaaca attaaatgtt
     3061 gaaaatgaca aacagctaat catctagtct gaattagcag atgattagac tgctatgtct
     3121 gtatttgtca attattgagt aacattacag gaggaaatta tattcatgat agctgttaat
     3181 tggaacacac aagaagatat gacgaatatg ttttggagac aaaatatatc tcaaatgtgg
     3241 gttgaaacag aatttaaagt atcaaaagac attgcaagtt ggaagacttt atctgaagct
     3301 gaacaagaca catttaaaaa agcattagct ggtttaacag gcttagatac acatcaagca
     3361 gatgatggca tgcctttagt tatgctacat acgactgact taaggaaaaa agccgtttat
     3421 tcatttatgg cgatgatgga gcaaatacac gcgaaaagct attcacatat tttcacaaca
     3481 ctattaccat ctagtgaaac aaactactta ttagatgaat gggttttaga ggaacctcat
     3541 ttaaaatata aatctgataa aattgttgct aattatcaca agctttgggg taaagaagct
     3601 tcgatatacg accaatatat ggccagagtt acgagtgtat ttttagaaac attcttattc
     3661 ttctcaggtt tctattatcc actatatcta gctggtcaag gaaaaatgac gacatcaggt
     3721 gaaatcattc gtaaaatact tttagatgaa tctattcatg gtgtatttac cggtttagat
     3781 gcacagcatt tacgaaatga actatctgaa agtgagaaac aaaaagcaga ccaagaaatg
     3841 tataaattgc taaatgactt gtatttaaat gaagagtcat acacaaaaat gttatacgat
     3901 gatcttggaa tcactgaaga tgtgttaaac tatgttaaat ataatggaaa taaagcactt
     3961 tcaaatttag gttttgaacc ttattttgag gaacgtgaat ttaacccaat cattgagaat
     4021 gccttagata caacaactaa aaaccatgac ttcttctctg taaaaggtga tggttatgta
     4081 ttagcattaa atgtagaagc attacaagat gatgactttg tatttgacaa caaataacaa
     4141 ttaaattaaa agaccttcac atgtaaaggg aaatagcgat tcgtttcgtc ttgtctccta
     4201 catgttgaag gtcttttttt atgtgtatct aactcattat tagactgagt cagaaatcaa
     4261 tgttctaaga tgtacaatgt tatttatatt ggcagtagtt ggcggggcct ccaacacaga
     4321 agctggcggg aactcagctt acaataatgt gcaagttggc ggggcctcca acatagaagc
     4381 tggcgggaac tcagcttaca ataatgtgca agttggcggg gccccaacat agaagctggc
     4441 ggaaagtcag cttacaataa tgtgcaagtt ggggcggagc ctcaacacag agaaattgaa
     4501 ttcccaattt ctacagacaa tgtaagttgg cggagcctca acataaagaa aaactttttc
     4561 tttagaaatt atcatttcca ctcatgtatt cctttttaag tacacattag ctgagtctaa
     4621 tgttaagaac cactacataa tcaatcatta gtagttttta tcatttccac actattccca
     4681 gacatcaaaa tcttaagtgt tctattttac tttaagtaaa caaaatacac attctgaaaa
     4741 attaaatttc agtttaattg caaatatcaa taaaattgac actaaattat ttgaaaggct
     4801 attgaaatta gggtcaaaaa acgctactat taatgagaaa tattatcaat gataatgatt
     4861 atcattaatt taaagggaga aaaatttgta atgaagtatt tattaaaggg aaatattttg
     4921 cttctattac taatgttgtt gacaattatt tcgttgttca taggtgtgag tgaactatca
     4981 attaaagatt tactacattt aactgagtca cagcggaata ttttattctc aagccgaata
     5041 ccaaggacga tgagtatttt aattgctgga agttcgttgg ctttagcagg cttgataatg
     5101 caacaaatga tgcaaaataa gtttgttagt ccgactacag ctggaacgat ggaatgggct
     5161 aaactaggta ttttaattgc tttattgttc tttccaaccg gtcatatttt attaaaacta
     5221 gtatttgctg ttatttgtag tatttgcggt acgtttttat ttgttaaaat cattgatttt
     5281 ataaaagtga aagatgtcat ttttgtaccg cttctaggaa ttatgatggg tgggattgtt
     5341 gcaagtttca caaccttcat ctcattgcgc acgaatgctg ttcaaagcat tggtaattgg
     5401 cttaacggga actttgccat tatcacaagt ggacgctatg aaattttata tttaagtatt
     5461 cctcttttag cattgacata tctttttgct aatcatttca cgattgtagg aatgggtaaa
     5521 gactttacta ataatttagg tttgagttac gaaaaattaa ttaacatcgc attgtttatt
     5581 actgcaacta ttacagcatt ggtagtggtg actgttggaa cattaccgtt cttaggttta
     5641 gttattccaa atattatttc gatttataga ggtgatcatt tgaaaaatgc tatcccacat
     5701 acgatgatgt taggtgccat ctttgtatta ttttctgata tagttggcag aattgttgtt
     5761 tatccatatg aaataaatat tggtttaaca ataggtgtat ttggaacaat cattttcctt
     5821 atcttgctta tgaaaggtag gaaaaattat gcgcaacaat aataaaaaaa taatgctttt
     5881 aattgcagta acgttattaa ttagtatgct gtacttattt gtaggtattg atattgaaat
     5941 atttgaatat caattttcaa gtcgtttaag aaagttcata ttaattattt tagtaggtgc
     6001 tgccattgca acttcagtgg tgatttttca agcgattaca aataaccgtc tattgacacc
     6061 atcaataatg ggcttagatg cagtttattt atttatcaaa gtattgccag tctttttatt
     6121 tggaattcaa tcggtatggg ttactaatgt atatttgaac tttatattaa cacttataac
     6181 gatggtgtta ttcgcactaa tcctattcca aggtatcttt aaaattggac atttttcaat
     6241 ttattttatc ttacttattg gtgtcctttt aggaacattt tttagaagta ttacaggttt
     6301 tattcaattg attatggatc ctgagtcatt tttagcaata caaagtagta tgtttgctaa
     6361 ttttaatgct tctaattcga atttagttac tttctcagca gtgctattag taatattatt
     6421 agtcattaca attttactat tgccttattt agatgtattg cttttaggtc gtgctgaagc
     6481 aattaatctg gggatatctt atgaaaaatt aacgcgaatt ctacttgtaa tagtctcagt
     6541 tttagtttct gtgtcaactg cattagtagg accaattaca tttttaggtt tattaactgt
     6601 aaatctagcg catgaactaa tgaagacgta tgaacataag tatattttaa ttgcgacaat
     6661 ttgcttgagt tggattagtt tatttagtgc gcaatgggta gttgaaaatg tgtttgaagc
     6721 tacgacagaa atgagtatac ttattgattt gattggtgga agttatttca tttatctatt
     6781 agttagaagg agaaatgcgc aatgattcaa gttggaaatt taactaaaac tataaataat
     6841 caaatgatat tggaagatat tagcatagat atcgaaaaag gtaaattgac ttctttaatt
     6901 ggacctaatg gtgcgggtaa gagtacttta ctttcagcga tatgtcgctt aattcggttt
     6961 gatgaagggg aagtgaaaat agatggacag ctaatgtctg attataaaaa taatgacttg
     7021 tcgaaaaaga tatcgatttt aaaacaaaca aaccatactg aaatgaatat tacggtagag
     7081 cagttggtaa actttgggcg attcccttat tctaaaggtc gtttgacgaa agaggatcat
     7141 gatattgtca atgatgcgct agatttgttg caactacaag atatcagaaa tcgtaatatt
     7201 aagtcattat ctggtggaca acgtcagcgt gcatatattg caatgacaat agcacaagat
     7261 actgaatata ttttgctaga tgaaccatta aataatttag atatgaagca tgctgttcaa
     7321 attatgcaaa cgttaaaaat gttagcgcat aaaatgaata aagcgattgt cattgtatta
     7381 catgatatta actttgcgtc ctgttattca gatcagattg tagcattgaa aaacggacaa
     7441 ctagttaaat cagatttgaa agataatgtc attcaaagta gtgttttaag tgatttatat
     7501 gacatggata ttcaaattga acatataaga aatcaaagga tttgtttata ttttaaggat
     7561 tgataatttg gagacacttt aaaggggtga tgcgccaatt aaagaagggt taaacgtaaa
     7621 gcatttattt atatttcaca tcaagcacac agattaagct aaaagaggag aataatatat
     7681 tatgaagaaa acagtcttat atttagtatt agcagtaatg tttttattag cggcatgcgg
     7741 taacaattct gataaagaac aatcaaaatc agaaactaaa ggttcaaaag aaacggttaa
     7801 aattgaaaat aactataaaa tgcgtggcga gaaaaaagat ggtagtgacg ctaaaaaagt
     7861 taaagaaact gttgaagtac caaagaatcc taaaaatgca gttgtgttag actatggcgc
     7921 attagatgta atgaaagaaa tgggcttatc agacaaagta aaagcattac ctaaagggga
     7981 aggcggtaag tcattaccga atttcttaga atcatttaaa gatgataaat atacaaacgt
     8041 tggtaattta aaagaagtga attttgataa aattgctgcg acgaaacccg aagtaatctt
     8101 tatctctgga cgtacagcta atcaaaagaa tttagatgaa ttcaaaaaag ctgcacctaa
     8161 agcgaaaatt gtttatgttg gtgcagatga aaagaactta attggttcaa tgaaacaaaa
     8221 cactgaaaat atcggtaaaa tctacgacaa agaagtcaaa gctaaagagt taaataaaga
     8281 tttagataat aaaatagctt caatgaaaga taaaacgaaa aacttcaata aaactgttat
     8341 gtatttacta gttaacgaag gtgaattatc aacatttgga cctaaaggtc gttttggtgg
     8401 attagtttac gatacattag gattcaatgc agttgataaa aaagtaagta atagcaatca
     8461 tggacaaaat gtttctaacg aatatgtaaa taaagaaaat ccagatgtta ttttagccat
     8521 ggatagaggt caagcggtaa gtggtaaatc aactgcgaaa caagcattaa ataatcctgt
     8581 attaaaaaat gttaaagcaa ttaaagaaga caaagtttat aatttagatc ctaaattatg
     8641 gtactttgca gctggatcaa ctacaactac gattaaacaa attgaggaac ttgataaagt
     8701 tgtaaaataa ttttaaaaga gggaaacaat ggttaaaggt cttaatcatt gtttccctct
     8761 tttctttaaa aaaggaaatc tgggacgtta atcaatgtcc tagactctat aatgttctgt
     8821 tgtcagtcgt tggttgaatg aacatgtact tgtaacaagc tcatttcaat accagtgggc
     8881 tccaaacata gagaatttca aaaaggaatt atacagaagt ggtgctttat catgtctgtc
     8941 ccacttccta taatgttttg actatgttgt ttatatttca aaataaatat gatagtgata
     9001 tttgcagcga ttgttaaacc gagattggca atttggacaa cgctctacca tcatatattc
     9061 attgattgtt aattcgtgtt tgcatacacc gcataagatt gctttttcgt taaatgaagg
     9121 ctcagaccaa cgcttaatgg cgtgcttttc aaactcatta tggcacttat agcatggata
     9181 gtatttattg caacatttaa atttaatagc aataatatct tcttcggtaa aataatggcg
     9241 acagcgtgtt tcagtatcga ttaatgaacc ataaacttta ggcatagaca aagctcctta
     9301 acttacgatt cctttggatg ttcaccaata atgcgaactt cacgatttaa ttcaatgcca
     9361 aatttttctt tgacggtctt ttgtacataa tgaataaggt tttcataatc tgtagcagtt
     9421 ccattgtcta catttaccat aaaaccagcg tgtttagttg aaacttcaac gcctccaata
     9481 cggtgacctt gcaaattaga atcttgtatc aatttacctg caaaatgacc aggcggtctt
     9541 tggaatacac taccacatga aggatactct aaaggttgtt tagattctct acgttctgtt
     9601 aaatcatcca ttttagcttg tatttcagtc agtttaccag gagctaaagt aaatgcagct
     9661 tctaatacaa ctaagtgttc tttttgaata atgctattac gataatctaa ttctaattct
     9721 tttgttgtaa gtttaattaa cgagccttgt tcgtttacgc aaagcgcata gtctatacaa
     9781 tctttaactt cgccaccata agcgccagca ttcatataca cagcaccacc aattgaacct
     9841 ggaataccac atgcaaattc aaggccagta agtgcgtaat cacgagcaac acgtgagaca
     9901 tcaataattg cagcgccgct accggctatt atcgcatcat cagatacttc gatatgatct
     9961 agtgataata aactaattac aatgccacga ataccacctt cacggataat aatatttgag
    10021 ccatttccta aatatgtaac aggaatctca ttttgatagg catatttaac aactgcttgt
    10081 acttcttcat ttttagtagg ggtaatataa aagtcggcat taccacctgt tttagtataa
    10141 gtgtatcgtt ttaaaggttc atcaacttta attttttcat ttgggataag ttgttgtaaa
    10201 gcttgataga tgtctttatt tatcacttct cagtaca
//
LOCUS       DAHHRR010000091        10126 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.091, whole genome shotgun sequence.
ACCESSION   DAHHRR010000091 DAHHRR010000000
VERSION     DAHHRR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.091"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..857)
                     /locus_tag="NBI42_001967"
     CDS             complement(<1..857)
                     /locus_tag="NBI42_001967"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCD1778369.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEVSQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRA"
     gene            1075..1596
                     /locus_tag="NBI42_001968"
     CDS             1075..1596
                     /locus_tag="NBI42_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HCD1778370.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLLQAIGGEGYVYVKG
                     YDDLQFFENFVLHSDERLKSSRKLDAIKDFKEIDSTDRVLLFSPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(1705..3315)
                     /locus_tag="NBI42_001969"
     CDS             complement(1705..3315)
                     /locus_tag="NBI42_001969"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCD1778371.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(3651..4181)
                     /locus_tag="NBI42_001970"
     CDS             complement(3651..4181)
                     /locus_tag="NBI42_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCD1778372.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIETRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEDFEDEEDFKA"
     gene            complement(4293..5153)
                     /locus_tag="NBI42_001971"
     CDS             complement(4293..5153)
                     /locus_tag="NBI42_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1778373.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINGFELNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVIAMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            5577..6380
                     /gene="coaW"
                     /locus_tag="NBI42_001972"
     CDS             5577..6380
                     /gene="coaW"
                     /locus_tag="NBI42_001972"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HCD1778374.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLGK"
     gene            complement(6510..7181)
                     /locus_tag="NBI42_001973"
     CDS             complement(6510..7181)
                     /locus_tag="NBI42_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HCD1778375.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGPIVSAQKLS
                     NELTF"
     gene            complement(7580..8773)
                     /locus_tag="NBI42_001974"
     CDS             complement(7580..8773)
                     /locus_tag="NBI42_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCD1778376.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPFVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            complement(8773..9957)
                     /locus_tag="NBI42_001975"
     CDS             complement(8773..9957)
                     /locus_tag="NBI42_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCD1778377.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKGQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGP
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
BASE COUNT         3202 a         1948 c         1386 g         3590 t
ORIGIN      
        1 ttagcgcggt ttgaagtacc gaactcttta attttacctt taactgtttc tttgatagct
       61 tcacgtgcag gtcctaagta tttacgagga tcgtaaactt ctttgtcgtt atttaaaacg
      121 tcacgaactg cttttgctga agcgatttgg ttttcagtgt ttacgttaat tttagctgta
      181 ccaaatggaa ttgctttttg gatatcttta gttgggatac cagtaccacc gtgtaatact
      241 aatggtaaac ctgtagataa accgatttct tccatttctt taaatcctaa ttttggttca
      301 cctttgtatg gaccatgaac tgaacctaat gctggcgcta atgcatcgat accagttttt
      361 tcaactagtt cttgacattc tttaggatca gcataaatga tgccgtctgc tacaacatca
      421 tcttcttgcc cgccaacagt acctaattca gcttctacag aaacaccttt ttcatgagcg
      481 tattcaacaa cttttttagt tgttgctacg ttttcttcga atgggctgtg tgaagcatca
      541 atcattactg atgtgaaacc agcatcgata gcttctttac atttttcaaa gcttgaacca
      601 tggtctaaat ggattgctac aggaatagtg atgtttaagt catgcattaa cccttcaacc
      661 attttaacaa ttgtgtagaa accgctcatg taacgagcag caccttcaga aacacctaaa
      721 attacaggtg cattttcttc ttgtgacact tctaaaattg cttgtgtgaa ttctaggtta
      781 ttaatattgt attgacctac cgcataacca ttttcttttg catcaattaa catttctttc
      841 attgaaacta aaggcatgaa agttcctcct tgagtgctta cgcacgtatt tttcaaagta
      901 attatatcaa aaaaatagcg tcaatgcatt gaaatactgc agattaaatg tgtatttttt
      961 gaaaaatgcg atagttatac taaatgcaaa cgtatacatt tttatgcaat ttaagtcatt
     1021 gcataatatg tgcccattaa ttacaatatt ttataagtag attggaggat aaagatgtca
     1081 aaaatcttaa acacacaatt aataggaatc tttaatagat tagaaaaaca atctttagaa
     1141 attcaaatgg cagcacaatg cttactacaa gcgatcggtg gtgaaggtta tgtttatgta
     1201 aaaggatatg atgacttaca atttttcgaa aattttgttc tacatagtga tgagcgttta
     1261 aaatctagcc gaaaacttga cgctattaaa gactttaaag aaattgactc aactgatcgt
     1321 gtattattat tctcaccatt ttataatgat caagttgcat tagatattca aaaacttatc
     1381 gacttagata tcgatgtggt cttaataagc aacaaaccta aaacagatga tttccctgat
     1441 catctcgtac attttatcga tttatctaca ccaagaccta tagtctatac tgaagattac
     1501 gataagattg tccagccaca tgcgatggca ttgaattatg tatactatga catttataca
     1561 caaatgattg aaatgacaag agacttagaa ttataaaagt cactattgtt atttaatacc
     1621 ttgtcactaa cataatatta aaattatacg aaaaaccggt actttcaatg tttaataatg
     1681 aaagtaccgg ttttattagc aaatttattt attttgttga tattttaatg aagcttcaat
     1741 aaatgattta aaaatcgggt gcggacgatt tggtctagat aagaattctg ggtggaattg
     1801 acaagcaata aagaaatcat ttgtcggaat ctctaccatt tctactaaac gtccatctgg
     1861 acttgtacca gaaatcacca taccattcgc ttctaattgt tctctatagt cattattaaa
     1921 ttcataacga tgacgatgtc tttcttcaat ttccgcttta ccataaacat cttgtgccaa
     1981 tgtaccttct ttaattgaac atggatataa gcctaagcgt aatgtaccac ctaaatcttc
     2041 gatatctttt tgttctggta ataaatctat aattgggtat ggtgttgcag ggtctaattc
     2101 agctgaatgt gcgccttcaa ggcctaatac gtttcttgaa aattcaactg ttgcaagttg
     2161 cattcctaaa caaataccaa agaatggtac attgttttct ctagcatact taattgcact
     2221 aattttacct tcacttgcac ggaaaccaaa tccacctggt actaaaatac cgtcgacatc
     2281 tgcaaggtat tcggccgcat tttcatctgt aacttcactt gaatcaatcc atctaatatc
     2341 aatatcttta gcgaaaggat atccggcatg ttttaatgat tcaactacag ataagtatgc
     2401 atcttgtaag ctaacatact taccaactaa accaattgta attttaccat ctaaattatt
     2461 aacgatatct aacaactgtt tccattcatc aagctgtgtt tcatatttcg cgtttaattg
     2521 taaacgttta ataacgatat catccatatt ttgttggctt aattgtaatg ggatttcgta
     2581 taaagagtct gcatcacgac attcaataac actttcttta ttaatgtcac agaataatgc
     2641 aattttatct tttaaatctt gtgtcatttc atattcagtt cttacaacga ttaagtctgg
     2701 ttgaatacct aagcctcgta attctttaac gctatgttgt gttggcttcg ttttcatttc
     2761 tccagcagct ttaatataag gtaatagtgt acaatgaaca tacataacgt tttctctacc
     2821 taaatcactg cgaatttggc gaatcgcttc aataaacggt aatgactcaa tatcacctgt
     2881 tgtaccgcca atttcagtga taacaacgtc tgcattcgta ctttcccctg caagtaataa
     2941 acgttcttta atttcatttg taatatgcgg aataacttga actgttccgc ctaagtaatc
     3001 accacgacgt tcttttttca atacgtgtga atacacttta ccggctgtta catttgaaaa
     3061 cttgtttaaa ttaatatcaa taaatctttc gtaatgtcct aagtctaggt cagtttctgc
     3121 accatcatcc gttacgaata cttcaccatg ttgataagga ctcattgtac ctgggtcaac
     3181 atttaagtat ggatcgaatt tttgaattgt tacatttaga cctctatctt ttaataatct
     3241 acctagagaa gatgctgtaa tacccttccc taatgatgaa actacgccac ctgttacaaa
     3301 aataaatttt gtcatgtctt gacctcctaa ttattaaagg gaatgcttcc tcatcaattg
     3361 taaaaattac gtaaaacata acatttgagt aagctcattc aagttgttta tttgtcgaat
     3421 tcgacaaaat taaattctgt ttacttaccg tttccatact gctatgtcac tatgtatatc
     3481 atttacaact ttgattattg cgttatcatt tcttgattta ctcaattaaa taaaaaaacg
     3541 ctccctctca caaattttgt aaggggagcg tataaatttt atacacgtgt cctatttaaa
     3601 ggagcccgaa taaaatttta tcatctttca actaaaagtc aaacaaaaaa ttatgctttg
     3661 aagtcttctt cgtcttcaaa atcttcttcg tcttcatcta gttcatcttc atcttcttca
     3721 atgactatat ccgaatgatt gatttcttct tcaacttgct catcttcagg atcatttaat
     3781 tcttcttgat catcagtttg agctggaata tcgtcatcat catccatttc atcttcaccc
     3841 aataatttta agttttgatc ttcttcatca tcggcgtcta aaatatcaaa tttttgaatt
     3901 gttggtgcaa ttttctcttc gatatcatct actgagtacc agtcacgtaa tccccataag
     3961 ttttcaccaa catttaaaaa acgtccatct gtatttaaat ctgtgtaaaa ttgtacaaca
     4021 cgagtttcaa tttcctcata ctcataatca cctaatgctc taaattcatc gatgatatca
     4081 tataagttca ttgtttcacc tttatcattc aataatgtat aagccatatc aataaatgat
     4141 ttttcatcaa ccatttgttt tgtataatct tgaattttca ttgcccaata cttcctttcg
     4201 aaggatataa tctaaattta tacgtctgaa ttcatttata tatagtaaca aaactaaatt
     4261 ataaatgaca atatcatatt tcgcaatcac tgttagtaat ttaaatattt ttcgaaaacg
     4321 acataatcat cagtttgttc gatttcaacg aaattactat ttagacataa tggtttaaaa
     4381 tcattattct ctccaaacag aatcatcgca atcacattaa ggttgtcata gttttttctt
     4441 aagtaagtag gcaattgtcg aagtgcactt gaaccaatac cattaaagcg atatgattta
     4501 tccacttcaa tagcagcaat gttcatgcgt tcaccaatct gctccaatgc gataaagcca
     4561 accttttggc catcaatcat taaattaatg atatgtacat tggtcacttc tgaacgactt
     4621 tcttcttcaa gctttaatga cgaaacatgt tttgaatcta agtccaagta atataatcgt
     4681 tctttgccaa ttggtccttc ttctttagtc gctgtatgca tgaatccagc acgttcgtat
     4741 aggttccaag cattgtcatt ttcagcatct actactagat aaagatgatt aaaatcagga
     4801 aatacacctt gaacatattg cggcaatgac atcattattt tcgtgccata tccaaaacct
     4861 tgatattttt cattaatcga taatgaacgt atataaacga cattttcagg tgtatcatag
     4921 ccttcatgct gatagtaacg atgtaataca aaaaagccca ccacttcatt tttatcgttt
     4981 attgctacat tcgcaatcct atcagcatca ttaattgcat catcaataac ttctttaggt
     5041 aaagatgaat atatttgttg tctttcattc aattcaaaac cattaattgc tgaacgatat
     5101 tgttcatcaa atgcttttaa agtgatacct tcaaaacaaa gttctttagc catatattta
     5161 acccctatct ttttatatat gaagcatgaa tccccatatt attttaccta ttatttcact
     5221 acgataccat tatttactct aagttaaata acttacttat ttatattttt caaattttaa
     5281 aattaatgta ctattcctca ttacttatca ttttaatcag agacttacaa atacactgcg
     5341 tacgaactac ttttaatatt acatctctaa ctaacaatcc tatacaactt caaaattcct
     5401 agaaaataat atatataact taacgtccac gtttgtaata caataattgc gatacgctta
     5461 caatgttgaa atacaatggc atcaaatttg ttaataatta ctattattac ttgtttttta
     5521 gtcattttac tatactatta tttaaggata gacattaata atgaggtggg aatcttatga
     5581 aagttggcat tgacgctggc ggtacactaa ttaaaatcgt tcaagagcaa gataaccaac
     5641 gtacttttaa aactgaatta actaaaaata ttgatcaagt tgtggaatgg ttaaaccaac
     5701 aacaaattga aaaattatgc ttaactggag gtaatgcagg tgtcattgct gaaaacatca
     5761 acattcctgc acaaattttt gtagagtttg atgctgcatc tcaaggacta ggtattttgt
     5821 taaaagagca aggccatgac ttagcagatt atatttttgc aaatgttggt accggtacgt
     5881 cactacatta tttcgacggc caatcacaac gtcgtgtagg cggtatcggt acaggtggtg
     5941 gcatgattca aggactcggt tatttactaa gtcaaatcac tgattataaa cagttaactg
     6001 acatggcaca acacggtgat cgtaacacta tcgatttaaa ggttagacat atttataaag
     6061 ataccgaacc acccattccc ggtgatttaa cagcagctaa ttttggtcat gtactacatc
     6121 atttagatgc tgattttaca cctagcaata aattagcagc cgttattggt gtcgttggcg
     6181 aagttgttac gacaatggcc attactgttg cacgtgaatt taaaactgaa aatatcgttt
     6241 atatcggttc ttcatttcat aataacgctt tattacgcaa agttgtggaa gattatactg
     6301 tgttacgcgg ttgcaaaccg tactatgttg aaaacggcgc attttcaggt gctattggtg
     6361 cattatattt aggaaaatag aaaaagacca tttatatgag cgttgtttta aataaacgct
     6421 atataaatgg ccttttattt tatggtctac gtgttttgaa tacgtattgt tccatttatt
     6481 atcacttaat tacttacaac tcaattcaat taaaatgtaa gttcattaga caatttttgc
     6541 gctgaaacaa ttggaccaac cacgccagtt tttaaatcta tcgcaacggc tttatgatca
     6601 tctctaagtt catctagcca ttctccgaaa acagaacctt caatcgtaat taaatcacac
     6661 tcttcaaaat cttcatctcg aacttttaac gctctgtttt taatactcca tacaggcata
     6721 atatgcggct ttttttcgtc gctatcattt tcactaatga gaataaactg cttacttcct
     6781 tttttagtta agccatatac ttcatcatat ttcgctacat cttggacaaa ttctttgatt
     6841 ctaattttat ctaattccgt catcacttct tgtgtaacag ctacaacgtt tagaagatgc
     6901 ccttcttctt ccattgtttg attcataata atatggtctt gttcatcaaa caattcatct
     6961 aattcataag tagcaaaacg atcaacgtcc atttttacaa ctttatcaaa tggaatatca
     7021 cgacttgtta aaaatgatac ggccacactt tcttctgtcc aaacgcctac atacgcttca
     7081 cttttataat ttaggcgtac tagtttttta tttttagagg cgaaataaat acattcattt
     7141 attaatatat ctctaaagaa tggatatgct ttataagaca tgcttgaccc tcacttttta
     7201 aaatatgtca tacataaaat agtaaatgac tccaacaaga tagtcaatta gacatattat
     7261 gaattcaatt taatgtcatg tttttcattt taaaaattca atagcattaa attttacgta
     7321 tacacaaatt cggtctataa cacgaaacat atctagttac taaaaatttc aaaacgataa
     7381 cctttaatat acctttatca acaaaatggc aactcttata acatagtgaa ataaaagtca
     7441 ccatttaata ttagtattac aaaatattgc catatcataa atttaattac tgttgactag
     7501 aatcaaaaaa agcttgttac aagtgcatat tcagtcaact actggcaaca taacattgta
     7561 gcgccttatg ttccgaactc taatagtgtt caagttcagc taaaaatgcg cgttcattct
     7621 ccagtactgt ttcttttgaa tctccatgcc aaatacagcc aaaccttgat ttaactttat
     7681 cttcaccgta ccaatcacca tcgtaataag ataacgggta taaactacct tctttgacat
     7741 atttcaaaat cgtattgaag aaatgtgtgt tatcaccttt tgaatgataa ctataaaact
     7801 tttgatatcc tgaattcaac tcgtttgcaa gtaataacat gctcgttgaa ccattttgtc
     7861 taaagtttaa atcaatcgca taaacgttat catcctcatc gacgagtaag tcaaaaccag
     7921 ccacaccaaa gaatccttga ttcacaccgt tttccataat ttgtctacct gcttctataa
     7981 cgtgctctgg tacattagta gtattttcat ttccgttata aaatccgtac ttatccgtta
     8041 attgcgttgc cgcaccaaga tattgaatgc ctagcgattc cgagtatgca aactgaacgc
     8101 aataattcgc tttttcttca atcttctgtt cgataataag cgaattcgtt tcagcagttg
     8161 cctctttaat acgcgttatc gctttttgta aatctgcatc atgataacaa atcataacgc
     8221 catagccacc tgctgtcgga agatcatctc ccggcttaat tacaaacggg aattcccaat
     8281 tcttaatctc gttttcgaat tgctcaattt ttacaacttt tctttttggt aaaaacttcc
     8341 catttgtcca ttcaggtatt cttgctttat tatttaaagc aacaaataac gttttatcta
     8401 atgcataata ttgctgattc aagattgttt catcatgaat atattgaaaa taaatctttt
     8461 tattttcctt atgtgccaat tgtttgatca agttttcgta agattgctga ttgttaaatg
     8521 tataaattga gttcggtact tccttaccaa taacttgaaa tagctgatgc aatttgtctg
     8581 tcgcactagc ttcgtgaaca ataacaggta attgatttgc tattaataac tccctaccag
     8641 ttaaaaaatt agattgatgt tcgtccggtt tcaaccatgg attcgatata tacgaaggtc
     8701 ttgacgtata gacaacatct ttgtcatata aatcacttaa cgttaagttc ggctcattac
     8761 cattatttgt cattacttcc cattcccttt caaatgcgca tgctcttcaa taatgtcttg
     8821 ataaacgtct tgatttgtaa ttaactctaa ccccatcaac gccattattt tagcaccttt
     8881 aattaatgct tcatcaccat gtacactcgc agctgcttct ctaaatctat gcgtatgtcc
     8941 tactaaatta cgtgatccta ttttaatatg aggatgtatt gttggcacaa catgacttac
     9001 gttccctgta tccgtagagc cataaccaaa atcatcatca ataactgctt caccaacttc
     9061 ttcagcatat ttagcaaata aatcatctaa tttcggcgtt ttaatgaatt cattcacacc
     9121 gttttgaatt ggaccaaatt cataatcaca accagtctgt atcgcagctc cacgtgcgat
     9181 ttgatttact ttttctgtta atatatccaa ttctttacgc gtcattgctc tagtataaaa
     9241 acgagcatgt gtatagtctg gaataatatt agctgctttc ccgccatcta aaatcacacc
     9301 atgcacacgt tgaccttttt taatatgttg tcgtagttgt gctacaccat taaaataact
     9361 aatcatagcg tctaatgcat ttaacgcttc atctgcattt tcagaggcat gagcactttt
     9421 tccgtaaaat ttaacatcta aaacatccac tgccaaagta tcaatcgttt tataagtttc
     9481 atttcccgga tgaatcatta aggcaatgtc tatttgatca atcacaccag ccttgacata
     9541 agaagcttta gcgctaccat tttccccacc ttcttcagct ggacatccga gaacgactac
     9601 tttaccacca atttggtcaa tcacttgctt caaaccaatt gcaccaagca cacttgcagt
     9661 tccaatgata ttatgaccac aagcatgacc caatcctggc aaagcatcgt attctgctaa
     9721 aaaacctata gctggcccgt caagacccga atcatatgtc gctataaacc cagttgcatg
     9781 cccagcaatc tcggtttcaa tttcaaaatc atgttctttc aaacgatcta ttaaagttcg
     9841 agacgcaaat atttcttcat taccaagttc aggacgttca tgaattctat gactaatttc
     9901 gatataacta tatttatttg tttctatata atcgagaatt tgttgttttt cactcatttt
     9961 ttactatctc ccttttaccc tacactcatt ttaatcatcc acaatatttt gttctttcaa
    10021 aatgaattat tacttattct atcggtttta tctcatgatg tcatctagtt tttctttatt
    10081 tacaaaattt tctaataact aaaagccttt cataaattta taaaac
//
LOCUS       DAHHRR010000092         9753 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.092, whole genome shotgun sequence.
ACCESSION   DAHHRR010000092 DAHHRR010000000
VERSION     DAHHRR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.092"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            73..708
                     /locus_tag="NBI42_001976"
     CDS             73..708
                     /locus_tag="NBI42_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCD1778378.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            1013..2092
                     /locus_tag="NBI42_001977"
     CDS             1013..2092
                     /locus_tag="NBI42_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCD1778379.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATGILQIPAHSFPILGTVIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIAIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVQQQSAKVQQQTQIV"
     gene            2152..2376
                     /locus_tag="NBI42_001978"
     CDS             2152..2376
                     /locus_tag="NBI42_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCD1778380.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLKSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            2584..3834
                     /locus_tag="NBI42_001979"
     CDS             2584..3834
                     /locus_tag="NBI42_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCD1778381.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFA
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            4017..4967
                     /locus_tag="NBI42_001980"
     CDS             4017..4967
                     /locus_tag="NBI42_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1778382.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQIEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVKQVDAIVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            5117..6601
                     /locus_tag="NBI42_001981"
     CDS             5117..6601
                     /locus_tag="NBI42_001981"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCD1778383.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYGEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            6598..7557
                     /locus_tag="NBI42_001982"
     CDS             6598..7557
                     /locus_tag="NBI42_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCD1778384.1"
                     /translation="MRRLFSIGEALIDFIPNITNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPRFVNDIDVNENDVVHFCSVDLVDSPMRVAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYNVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(7745..7851)
                     /locus_tag="NBI42_001983"
                     /pseudo
     CDS             complement(7745..7851)
                     /locus_tag="NBI42_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7923..8639)
                     /locus_tag="NBI42_001984"
     CDS             complement(7923..8639)
                     /locus_tag="NBI42_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1778385.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(8658..>9753)
                     /locus_tag="NBI42_001985"
     CDS             complement(8658..>9753)
                     /locus_tag="NBI42_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCD1778386.1"
                     /translation="IIFLYFKIRLYSVFLVMVTQIILYCANFVYIIIFSYIITISHSV
                     FIVLPIFLVVYVSISYALAYILNRILKRINGTYLSLNKKFLTVITIVIVITFSLLFAY
                     SQIDASDASTIKQYSLLFLGIIILLSILIFISSQFTLKEMKYKRNQEEIETYYEYTLK
                     IEAINNEMRKFRHDYVNILTTLSEYIREDDMNGLRDYFNKNIVPMKDNLQMNALKLNG
                     IENLKVREIKGLLTAKILRAQEMNIPISIEIPDEVTRINLNMIDLSRSIGIILDNAIE
                     ASSEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTL
                     KEIADNADNVLLDTIIENGFFIQKVEIINN"
BASE COUNT         3404 a         1341 c         1805 g         3203 t
ORIGIN      
        1 tctaacaatt acgctctaac tcgtttgaat acatagatat aattcattca aacacgaagg
       61 aggaaatagc gaatgagtga ccaagttaaa attcctcgag caactttaaa acgtttgccg
      121 ttatattata gatttgtcag ttcattaaaa tctaaaggta tagatcgtgt aaattcaaaa
      181 gcgattagcg atgcgttaca aattgactcg gcaacaattc gtcgtgactt ttcatatttt
      241 ggcgaattag gtaaaaaagg gtacggatat aatatagata gtttattgga tttctttaaa
      301 tctgaactaa gcgagagtga catgatcaaa atcgcaattg tcggagttgg gaacctaggg
      361 aaagctttgc tcacatataa cttttcaata catgacgata tgacgattac agaagcgttt
      421 gacgtaaaag aagatgttat tggccagaaa atagggaacg ttattgtaaa agataacgat
      481 gaattaataa caacattgaa gaaggaagaa atagatgttg tgattctaac tacaccagaa
      541 agagttgcac agaaagttgc agatgaactc gtccaagctg gtgtgaaagg tattttaaac
      601 ttcactcctg gtagaattaa tacgccttca gatgtgcaag tacatcaaat tgacttaggt
      661 atagaattac agtcattatt attctttatg aaaaattaca gtgaataaat gtgatgttag
      721 ctttgaatga ttaaccttgt tatatcttta attaattata gaacagtctg ggactaagta
      781 tgtctcaggc tttttctgtt atgtttgaaa atgaaaggaa aaaatgttgt gttgataaat
      841 atagtatagg ttaataaatt aacacgtaga gatataatga tgaatcgtaa ttaatcgcat
      901 ttcttatgtg aatataatat aaatatcaaa aatgttgtgt ataaggttga aaacaagaaa
      961 atcatcgcct ataatacata ctaaacttat cggaaatcag ggggttagga cgatgtggat
     1021 gatttttagt ggcttaattg tcgggggact cttaggtttt gtgatgcaaa gaacaagatt
     1081 ttgtttaaca ggtggctttc gagatatgta tgtgcaaaag aataataaga tgttctatgc
     1141 attattaatc gctattacta ttcaaagtat aggattattg attttgacgg caacaggtat
     1201 tttacaaatt cctgcacata gttttccaat attgggaaca gttataggtt cttttatttt
     1261 tggaattgga atagtattgg ctggagggtg tgcaacaggt acttggtatc gtgctggtga
     1321 agggctaatt ggtagttgga ttgcattagt attatatgct gttactgcag caatcactaa
     1381 aacagggatt ttaaagccag taatggataa aataaatcaa ccaacgaatg taaatagtga
     1441 tatgtctcaa acaactggca ttccgttttg gggattagtc gtcatattaa ctataatcac
     1501 catttttcta gttgtaagaa cactcaataa taagaaagtt cgagttgctg ttccaaaact
     1561 aaagcaacgt tatacaggta ttagatatta ccttttcgaa aaacgatacc atccatttat
     1621 tgcagcaatt gcaattggac ttatcgcact cttagcttgg ccaatgagtg catcaactgg
     1681 aagaaatgac ggtttaggta taacaacgcc atcagcaaat ttagtacact ttttgattac
     1741 aggtgaaact aaatttattg attggggtgt ctttttagtt ctaggaattt tcattggttc
     1801 atatattgca gctagaggat caagagaatt taaatggcga ttgccagaca agattacaat
     1861 acgaaacagt gctattggtg gcatatgtat gggatttggt gcgtcagttg ctggtggttg
     1921 ttctatcggt aacggtttgg ttgaaacggc aacgatgact tggcaaggat ggattgcgct
     1981 agcatcgatg atagttgggg tatggacaat gagtcatttt atctttgttc gtccaatgaa
     2041 aaaagtacaa caacaatctg caaaggtcca acagcaaacg caaatagtat agaagattat
     2101 tatgcaaatg atgttgatca aataaaagtg attggaaaag gagaaataat tatgatacac
     2161 gaattaggta cagtaggaat ggtatgtcca tttccgttaa ttgaagcgca aaagaaaatg
     2221 gcaacattga aatctggaga tgaattaaaa attgattttg actgtacaca agcgacggaa
     2281 gctattccaa attgggctgc agaaaatgga tatcctgtaa caaactatga acaaattgat
     2341 aatgcttcat ggacaattac aattcaaaaa gtttaacgtt atcattttaa caataaaata
     2401 gatattagat tctatggcta cttccgctac tttaaaagtg agtaagtagt cttttttgtt
     2461 ttagttcatg aaatcatttt tatatagtgt ggcacatttt attccaaaag atgtaataaa
     2521 acttaacgca tttttgcttt ttataaattg tcagattatt atgaaaacaa gggagtggta
     2581 agtatgaatc ttaacgatac gatatttatg tttttgtgta cattattagt ttggctaatg
     2641 acaccaggat taagtttatt ttatggtggg ttagttcaat ctaaaaatgc gcttaatact
     2701 gtcatgcaaa gtatggcagc aattgtgctt gttacatttg tatggataac agttggtttt
     2761 acaattagtt ttgggaatgg gaatttatgg ttcggaaatt gggaatatac ttttcttaat
     2821 catgtaggtt ttgcgactca agaagatatt agcccacata ttcctttcgc tttgtttatg
     2881 ttatttcaaa tgatgttttg tacgattgca atttctattt tatctggttc aatcgctgag
     2941 aaaatgaagt ttattcctta tttattattc gtagtaatat ggactgctct tgtatacagt
     3001 ccagtagcac attgggtttg gggcggcggt tggattaaca aactcggtgt attagatttc
     3061 gctggaggta ctgttgttca tattacatca ggtgtttctg gtttagtatt agctattatg
     3121 attggaaaag gaaacaaaca ttctgaatca acaccacata atcttatcat tacgttgatt
     3181 ggcggtatat ttgtgtggat tggttggtat ggatttaatg taggtagtgc ttttgcattt
     3241 gataatattg cgatgcttgc atttacaaat actgtcattt cagccagtgc aggtgctata
     3301 ggttggttaa ttctagaata tatttttaaa aagactacaa gtttactagg attattacta
     3361 ggtgcattag ctggtttagt tgtcattaca cctgcagcag gatacgtgac atatcttagt
     3421 gcaacaataa tggctttaat aggaggtatc tgttgttata ttgtcattaa ttacatcaag
     3481 gtaaaactaa aatatcatga tgcattagat gcatttggta ttcatggtgt tggtggtatt
     3541 attggtgctg ttttaacagc agttttccaa agtaaaaaag ccaatcctga cattgagaat
     3601 ggctttattt atactggtga catacatatt atacttgtac aaatattatg tgtaacagca
     3661 gttgtaattt ttagtatcgt catgacgttt attattgcga aagtaattaa attaattaca
     3721 ccattatctg ttacggaaca agaaacgaat ataggattag acaagattgt tcacggtgaa
     3781 catgcttact ttgaaggtga gctaaataga ttcaataaac atattcgata ttagaatata
     3841 tttacatcga atattcattg tcctgacatt taactaaagg ttgatgttgg gacattttgt
     3901 tatacaaaag ttttattttg aaatcttttt atgaaagaag cagaaatatt atttaaagcg
     3961 gttacacata tgctaaaata aggctaagtg tcacaaataa tgataggtga ataagtatga
     4021 aaaatatatc tgatattgcc aaattggcag gcgtttcaaa aagtacagta tctagatttt
     4081 taaataatgg atctgtcagt aaaaaaacaa gtgaaaaatt aacaagaatt atagcagaac
     4141 atgactatca accgaatcaa tttgctcaaa gtttaagagc gagacaaaca caccttattg
     4201 gtgccattat acctagaatg aattcatatg cagtagatga aacaatcaaa ggattggcaa
     4261 aacaatgcca aaaatatgaa tcacaattaa ttttaaatta cacaggatta aatatcgaag
     4321 cagaaataca agcgcttgaa acattagcac gcagtaaagt agatggtatt gttttaatgg
     4381 ctacagacat aacagagaga catatagaag tcattaataa aatgaatgta ccaatcgtta
     4441 ttgttggtca acaacatgaa caacttcata gtattgtgca tgatgattat aaagcaggtc
     4501 aaattatagg tgaatggatt ggtcaacagg gatatcaaca aattgaagtg tttagtgtga
     4561 gtgaaaaaga tattgcagtt ggtatacata gaaaacgtgg tttacttgac cagttagcca
     4621 aatatcaaat taaacctaat atacatgaaa caaattttac ttatgtggaa gcacaaaaag
     4681 atgttgcaaa tgttttggaa aatgtaaagc aagtagatgc aattgttggt gcaactgata
     4741 cgattgcatt agctgcctat aaatattatt ctgataaaaa agatgttatg aaaccacatc
     4801 aaatatatgg ttttggtggt gacccaatga cacaattagt gtctccatcg ataaaaacaa
     4861 ttcattataa ttattttgaa gctggccaat gcgcgatgga agagatacaa cagatgctta
     4921 aaaagcaaga tatgccatat agcgtcacag tagatgttaa tatttagacg ctgtattttt
     4981 ttaaaataaa tgtggaaccg ataccatata agtataaacg gataggttaa aagttaaaga
     5041 acgtaggtaa aatttgctat aatagaatat aaattgttaa cagcataaat tataaaagga
     5101 ggactgggta aatattatga ccgaatggac tagagaagaa cgttatcaac gaatcgagga
     5161 cgttgatact gagtatttta aaacattaaa acaacaagtt gatcaatcaa aatttcgtca
     5221 acaatttcat atacaaccag aaacaggctt attaaatgat cccaacggac ttatttttta
     5281 taaagggaag tattatgttt cacatcaatg gttcccgtta ggcgcagtac atggcttaaa
     5341 gtattggtat aactacacga gtgatgactt aataaacttt aaagctgaag ggccaatatt
     5401 aaatccagat actaaatatg acagccatgg tgtttatagc ggtagcgctt ttgaatataa
     5461 cgggcattta tattatatgt acacaggaaa tcatcgagat aatcattggc aacgacatgc
     5521 gagtcagatg atcgcacgat tgaaagaaga cggttcagtt gaaaagtttc caaagccagt
     5581 aattagccag caaccagaag gatatacgag tcattttaga gatcctaaag tttttaaata
     5641 tggtgagaaa tattatgcga tcattggcgc acaaaataat gatcagcaag gtcgattatt
     5701 actttataat actgaagata taattaattg gcattattta ggtgaaataa atacagagtt
     5761 agatgatttt ggatatatgt gggaatgccc agattacttt aatgtagata atcaagatgt
     5821 catacttatt tgtccacaag gtattgaacc aaaaggcgat cagttcaaaa atatttatca
     5881 aagtggttat atacttggaa agtttgatat tgaaaagtta acatatgaac atgaaaattt
     5941 tgtcgagctt gataatggct ttgatttcta tgcacctcaa acatttttag atgaaaaagg
     6001 ccgacgagta ctaattggat ggatggggtt accggaaatc gaatatccta ctgataatga
     6061 aggatgggcc cattgcttaa caattcctcg tgtattaaat gtagaaaatg gtcaacttaa
     6121 gcaacgtcca tatccagcgt tggaaaaatt acgtcacaat aaagagacag cattaggcta
     6181 cgcaaataaa tttactcgaa aattacatcc gtatgaaggt aaacagtatg aattaatcat
     6241 agatattttg gataatgatg ctaccgaagt gtactttgaa ttacgtacat ctaagacttc
     6301 ttcaacatta atcgcttata acaagcgtga aaataaaata acattagatc gcagcgacag
     6361 tggtttattg ccgacaaatg ttgaaggaac gacgcgtagt acgatattag acacgccatt
     6421 aaagcaatta caaatttttg ttgatacatc tagtatagaa attttctgta atgatgggga
     6481 acgtgttttg acatctcgaa ttttcccgac agaagacgca ctaggtatta aaacatcaac
     6541 agaatcaggg caagtatatt tacaatttac taaatatgat ttaaaggatg aacataaatg
     6601 agacgtctat tttcaatagg agaagcgtta attgatttta ttccaaatat tacaaatgcg
     6661 aatttaaaag atgttcaaac atttacgaag caaattggtg gcgcgccatg caatgtagct
     6721 tgtacggttc aaaagttagg acaacaagcg tatatgatta cacaattagg taatgatgca
     6781 tttggagata gcattattga aacgatatca agtattggtg tagatgtatc aaaagtgtat
     6841 cgcacgaatg aagcaaacac tgcgttagct tttgtgagtt tgactgaagc gggcgagcga
     6901 gatttttctt tttatcgtaa gccctctgct gatatgttat ttgaaccgcg ttttgtgaat
     6961 gatattgatg tgaatgaaaa cgatgtagta catttttgtt ctgttgattt ggtcgatagt
     7021 ccgatgagag ttgcgcatta tcaattgatt accaaaacat taaatgcaaa tggtactgtc
     7081 gtatttgatc ctaatgtaag attgccatta tgggataacg ctgaagattt aagacaaact
     7141 attcatacct ttttgccatt ggcgcatatt gtaaaagttt cagatgaaga attagaattt
     7201 attacaggta ttcatgatga aaatgaagcc attcaatcat tatttacagg taatgttact
     7261 gtagtcattt atacgaaagg agcagatggt gctgccgtat atttgaaaaa tggtattaat
     7321 cattatcata gtggctataa tgtaaaacct gttgatacaa ctggggcagg ggatgcattt
     7381 attggtgcag ttattagcag gattttagca accgatgtgt taaatttaac tcagctattt
     7441 gaaaatgaag gtgaggaaat tttagcgttt agcaatcgcg ttgctgcaat agtgacaacg
     7501 aagtatggtg ctattaatag tttgccaaca ttacaagagg ttgaacaagc attttaaata
     7561 attcatttat aaaacagtgt aggaagcaag ttgaatcttg tgctacactg ttttttaaaa
     7621 caaaataaga ttcacggggt aggaaattgt agcattttat ttaaggaaac tgtcgatttc
     7681 aaatttagta tattttgtta aaaattgcac cataggattg tagagtgaaa tagaaattga
     7741 taaattatgt agtaatagga ataggtatac aaagaaacgt tacatcattt tttaattcac
     7801 acgtcatatt tattttgtta ttaaaattaa aaagccagct atacagtgca tttgctagtt
     7861 atcttgttaa aatccaacaa gatttacaat tgaatacgcc gttaactgac tttattatct
     7921 tattatattt ttttaacgtt tctcaccgat gcatagcagt gttctttatt tttaaaatag
     7981 acaattcgct ctttcgaatc tatagattca atgttatggc gattgacgac aaagctatta
     8041 tgacatctaa agaaacgatc atctaattga ctcagttctt ttaaattacc ataaaattca
     8101 atttgacggt tatctaaatg ggcaatgagt ctgtgagatt ttgttgatga ttcaaaaaac
     8161 ataatatcat catattgaac atacactgaa ttactgccac gttttaattc aatcgtttca
     8221 acgctattat ctttagacaa caattgtaag cgtgtatgtg cagtttctaa acagtctata
     8281 attcgagttc ttaattcagc tggatcatct ttaaaaataa aatccatcgc tgcaactttg
     8341 tagacaaacg ttaaataggt aagttcgctg tgactcgtaa caaatataat gttaccaact
     8401 ggatcatgct tacgaatttc actgcctaat ttgataccat taatatcagt tgaaagttga
     8461 atatctaaaa agtaacagcc tatgtcattc atatttttag cttgctcaag cacctcataa
     8521 ggattatcag ttgcgagggc aatttccata ggcttttctt ctatcattat ataattttta
     8581 ataatggtaa ccatgttttc tctttgtttt ggatcgtctt cgcaaatgaa aattttcata
     8641 cattcacatc cttatggcta gttgttaata atttcaacct tttgaataaa gaaaccattc
     8701 tcgataattg tatctaataa gacattgtct gcattatcag caatttcttt taaagttgat
     8761 agacctaaac cacgaccttc acctttagta gaaaaacttt cttggaacaa ttcatgaata
     8821 cgtggtatat catctgcaca tttattcata acaataaacg ttactgaatt ttcgctttca
     8881 ataaatgcaa ctcgaatgat aggatcatca atttcacttg atgcctctat tgcgttatca
     8941 agaataatac caatactgcg acttaaatcg atcatattca aattaatgcg agttacttca
     9001 tctggtattt ctatactaat cggtatattc atttcttgtg cacgtaaaat tttagctgtt
     9061 agaagacctt taatctcacg tactttaaga ttctcaatac catttaactt aagagcattc
     9121 atttgtaaat tatctttcat aggtacaata tttttattga aatagtctcg aagaccgttc
     9181 atgtcatctt cacgaatgta ttctgaaaga gtcgtcaaga tattgacata atcatgacgg
     9241 aacttgcgca tttcattatt aattgcttca atctttaatg tgtattcata ataggtttca
     9301 atttcttctt gattacgttt atatttcatt tctttaagag taaattgaga agaaataaaa
     9361 attaagatac tcagtaatat aataattccc aagaataaaa gcgaatactg tttgattgtt
     9421 gatgcatcac tcgcatcaat ttgcgaatat gcaaataaca aagagaatgt tattactata
     9481 acaatagtta taacagtaag gaatttttta ttaagagaaa ggtatgtacc atttattctt
     9541 tttaatattc tatttaaaat gtaggcaagt gcataagaaa ttgatacata cactaccaaa
     9601 aagattggta gaactataaa cacgctgtgt gaaattgtta taatgtatga aaaaataatg
     9661 atataaacga aatttgcaca atataaaatg atttgagtaa ccattactaa aaatacagaa
     9721 tatagtctaa ttttaaaata gagaaaaatt att
//
LOCUS       DAHHRR010000093         9676 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.093, whole genome shotgun sequence.
ACCESSION   DAHHRR010000093 DAHHRR010000000
VERSION     DAHHRR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.093"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            272..1339
                     /locus_tag="NBI42_001986"
     CDS             272..1339
                     /locus_tag="NBI42_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778387.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYVTLLLDFNKIVRALGVVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKKYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            1490..2533
                     /locus_tag="NBI42_001987"
     CDS             1490..2533
                     /locus_tag="NBI42_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1778388.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQTTNENHANKDKI
                     NIKNKNARIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNNQLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            2862..3290
                     /locus_tag="NBI42_001988"
     CDS             2862..3290
                     /locus_tag="NBI42_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778389.1"
                     /translation="MTRSALKPFKNKRVMVTGRIHRVLFKNYLDRHSTFKPNVRILLK
                     DVIVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGKLVTEEAYIQNNQYQDKIYEKLPNIDFRLEDFYSKEN"
     gene            complement(3497..4003)
                     /locus_tag="NBI42_001989"
     CDS             complement(3497..4003)
                     /locus_tag="NBI42_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1778390.1"
                     /translation="MAHIIRRVSIKDVENFISMLAKIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            4116..5039
                     /gene="graX"
                     /locus_tag="NBI42_001990"
                     /gene_synonym="apsX"
     CDS             4116..5039
                     /gene="graX"
                     /locus_tag="NBI42_001990"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HCD1778391.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFTISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTTVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIVETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            5055..5729
                     /gene="graR"
                     /locus_tag="NBI42_001991"
                     /gene_synonym="apsR"
     CDS             5055..5729
                     /gene="graR"
                     /locus_tag="NBI42_001991"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HCD1778392.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAIIDLSKDSIQKGDDTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            5722..6762
                     /gene="graS"
                     /locus_tag="NBI42_001992"
                     /gene_synonym="apsS"
     CDS             5722..6762
                     /gene="graS"
                     /locus_tag="NBI42_001992"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HCD1778393.1"
                     /translation="MNNLKWVAYFLKSRKNWIFWILFLNFLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            6906..7667
                     /gene="vraF"
                     /locus_tag="NBI42_001993"
     CDS             6906..7667
                     /gene="vraF"
                     /locus_tag="NBI42_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HCD1778394.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEVLNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            7657..9546
                     /gene="vraG"
                     /locus_tag="NBI42_001994"
     CDS             7657..9546
                     /gene="vraG"
                     /locus_tag="NBI42_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HCD1778395.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMFEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKFIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAVIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
BASE COUNT         3543 a         1251 c         1673 g         3209 t
ORIGIN      
        1 ttaactgaga aattttgata tgtatttttc gaaatttaac taaaaatacg taagtcaaga
       61 agcacaatta ttaatcattt ttcctataca aaagttcgta tgactgcatt ataaaagcat
      121 aaatttataa tttttttaaa tgtcattgaa cgtgataatg tgaatggatt gagcaatttt
      181 gaaaaagtga aaaataacct atgcgacttg caattaattt tcagtacgtt ataatgcaca
      241 ctgtgcaaaa ttaaggaggt ctattattca catgatgatg aataaagaag caacaaaaat
      301 tggatttgcc tacgtcggca ttgtagtggg cgcaggattt tcaacaggac aagaagttat
      361 gcaatttttc actaaatatg gcttgtgggc ttatttaggt gtcattatat ctggttttat
      421 tttagctttt attgggcgcc aagtagcaaa aattggtact gcctttgaag cgacaaacca
      481 tgaatcaaca ttacaatacg tattcggtga aaagtttagt aaagtctttg attatatttt
      541 aatcttcttc ttatttggta tagctgtaac catgatagct ggtgcaggcg caacatttga
      601 agaaagttat aacataccta catggctagg tgctttaatt atgacattag caatttatgt
      661 aacattgcta ttagacttta ataaaatagt acgtgccctt ggtgtcgtca caccattttt
      721 aattgtttta gttgtattaa tcgctggtgt ttatttgttt aaaggtcatg tttcattagc
      781 agaagttaac caagtagtgc ctgaagcaag tatctggaag ggaatctggt ttggtacaat
      841 atatggtgga ttagcttttt ctgtaggttt tagtaccatc gtagcaatcg gtggggatac
      901 tgaaaagcgt acagtgtcag gtgcaggcgc gatgtatggt ggtattatct atactgtact
      961 actagcattg atcaactttg cattgcaaag tgaatatcca actattaaaa atgcctcaat
     1021 tccaacattg acgttagcaa ataatatcca tcctttaata gcaacagtgt tatctgttat
     1081 tatgctggcg gttatgtata atactattct aggactaatg tattcatttg cagcacgttt
     1141 tacagaacca tacagtaaaa aatatcatat ctttattatt ataatgatgg tagcaggtta
     1201 tttattaagt ttcgtaggat ttgctgaatt aattaataag ttatatacaa ttatgggata
     1261 tgtaggctta tttattgtag tagctgtaat tattaaatat ttcaaacgta aaaatgcgga
     1321 taaaaaacat attgcttaat atcatatgat ggatatccga aactttacaa ttgaatcact
     1381 ttgttttaac cttaaaagca attcgtctct actcttatcg ggcgaattgc tttttatatt
     1441 tattcagtct tttaatatga gcgtctaaca aatagagagg tacgatgtaa tgaataaaga
     1501 taataaatgg acgatgataa ctgcgctttt tataactgta atcagtgtat tgttagcatt
     1561 tcatctgaaa caacattatg accaaactac aaatgagaac catgctaata aagacaaaat
     1621 taatattaaa aataaaaatg cgcgcattta tcaaaacctt acatacaata gagttttccc
     1681 taacagtaaa ttagatatta ttacacctgt tgatatgtct tcaaatgcca aactgccagt
     1741 tattttttgg atgcacggtg gtggttatat tgcgggtgat aagcagtata aaaacccatt
     1801 attagcgaaa attgctgaac aagggtacat tgttgtgaat gtaaattatg cattggcgcc
     1861 acaatataaa tatcccactc cattaattca aatgaatcaa gcaactcaat tcattaaaga
     1921 aaataaaatg aatttaccta ttgattttaa tcaagtaatt attggcggtg attctgcagg
     1981 tgctcaatta gctagccaat ttactgcaat acagacaaac aatcaattaa gagaagccat
     2041 gaaatttgat cagtcattca aaccatcgca aattaaaggt gctatactat ttggtggttt
     2101 ttataatatg caaacagtta gagaaactga gtttccaaga atacagttat ttatgaaaag
     2161 ttatactggc gaagaagatt gggaaaagag ttttaaaaat atttcacaaa tgtcgacagt
     2221 aaaacaatcg acaaaaaatt atccaccaac atttttatct gttggagata gcgatccatt
     2281 cgaaagtcaa aatatagaat tcagtaagaa attacaagaa ttgaatgtac cagtagatac
     2341 tttgttttat gatggtacgc atcatttaca tcatcagtat caatttcacc ttaataaacc
     2401 tgaatcgata gataatatca aaaaagtgtt acttttctta agtcgtaata catcttctag
     2461 tggtattcaa actgaagaga aaccacaaat agaaaatccg agtaatgaat taccgttaaa
     2521 tcctttaaac taatgataaa cagtagtaat ttattactta agcaacattt aagatcttca
     2581 aattgaaaac gaagaattta aaacatgtgg ggctaatgtg taagtgtctg agatataata
     2641 aattgttagc ggttctttat catttctgtc acaccctatt atgatgtgac atttatttac
     2701 aaactaattt gttttgaacc tgaaaataac tttttacaac taaattgtcg aaaaacagtg
     2761 tgtacatgat ataataaatt tataaattga aaagaattca aaagaaaaat taaaatatag
     2821 attgagcacg aagtgatttg aaaataaggt tgtgaaaggg aatgacaagg tcagcattaa
     2881 aaccatttaa aaataaacgc gttatggtta ctggacgtat acatcgtgtt ttgtttaaaa
     2941 attatttaga tagacatagc acatttaagc cgaatgtaag gatattatta aaagatgtaa
     3001 ttgtttcagg tgtttcaata gatcatttat ggctatatga gacaaataaa tactatgcat
     3061 tggcaatgga acttattcat caacgagtaa aatttagtgc gaatgttgta ccgtattaca
     3121 aaataaatag aaataataat ttattcgtac aagattatgg aattaagcgt aaaggtaagt
     3181 tagttactga agaagcttac attcaaaaca atcagtatca ggataaaata tatgaaaaat
     3241 taccgaatat agattttaga ctcgaagatt tttatagtaa ggaaaactaa ataatatata
     3301 aagcaccact aagcgtagtt gatagttgat caattacaca ttagtggtgc tttattttga
     3361 atttaaattt gataaatgaa taagagtata cgcattcatt taagattaga tgacattgat
     3421 aaggatgagt aatgtattag ataaccatta aattcgttgt tatgcgattc tgatacaaga
     3481 ttatgataaa atagttttaa ttaagaattt tagccatcat atagtcatcg taatatttac
     3541 catcgataaa taacttatct tttaaaacgc cttcgatttg aaaatcggca cttttaaaaa
     3601 gttcgagggc aggttggtta ttgagtggta catttgcttc aattcggtgt atttgattat
     3661 ttaaacacca agccataatg gcatcaagaa gtgcttggcc aattccacga tgttgatata
     3721 atttctttac acctaaatca attttagcaa catgtttaat gcgttgaaat ggtgtcgtat
     3781 taacaaaggc aaagccaacg agttgttcat cactttcagc aacaaagatg actttatgtg
     3841 gagaagtgat atattcttct aattgtttac tagctgatgt gacgctagga tcatattctc
     3901 ctggtgtgta gaacatatac ggagattcat cgtatatttt cgctaacatt gaaatgaaat
     3961 tttctacatc tttgatacta actctacgta taatatgggc cataaaaagc ctccagtatt
     4021 ttgaaatttt aaaacatttg ctactattat aatatatatg taactaaaag gtggagtaat
     4081 atgttttttg gttacagttt aaaggagtat tttagatgaa acctaaagtt ttattagcag
     4141 gtggaacagg atatattggt aagtatttaa gtgaagtgat tgaaaatgat gctgaacttt
     4201 ttactatatc aaaatatcca gacaataaaa aaacagatga tgttgaaatg acttggattc
     4261 agtgtgatat atttcattac gaacaggttg ttgcagcaat gaatcaaata gatattgctg
     4321 tattctttat cgacccaaca aagaattctg ccaaaataac acaatcatca gcaagagatt
     4381 taacattaat cgcagcagat aattttggtc gagcagcggc tatcaatcaa gtaaaaaaag
     4441 taatctacat acctgggagt cgttatgata atgaaacaat tgaacgccta ggtgcatatg
     4501 gtacaactgt agaaacaaca aatttagttt ttaaacgttc tttagttaat gtagaattac
     4561 aagtttcaaa gtatgatgat gttagatcaa cgatgaaggt agttttacca aagggatgga
     4621 cattaaagaa cgttgtaaac cactttattg catggatggg ttacactaaa ggaacttttg
     4681 tgaaaacaga aaaatcacat gatcaattta agatatatat taagaataag gtgcgaccgc
     4741 tcgcagtatt taaaatagta gaaacagctg atggaataat aactttaatt ttattgagtg
     4801 gaagtttagt gaaaaaatat acagttaatc aagggaagtt agaatttaga ttaatcaaag
     4861 agtcggcagt cgtttatata catctatacg attatatccc tcgattattt tggccgattt
     4921 attactttat acaagcacca atgcaaaaaa tgatgattca tggctttgaa gttgactgcc
     4981 ggattaaaga ttttcaaagt cgattaaaat caggagaaaa tatgaaatat actaaatgat
     5041 attgggtgat atggatgcaa atactactag tagaagatga caatactttg tttcaagaat
     5101 tgaaaaaaga attagaacaa tgggatttta atgttgctgg tattgaagat ttcggcaaag
     5161 taatggatac atttgaaagt tttaatcctg aaattgttat attggatgtt caattaccta
     5221 aatatgatgg gttttattgg tgcagaaaaa tgagagaagt ttccaacgta ccaatattat
     5281 ttttatcatc tcgtgataat ccaatggatc aagtgatgag tatggaactt ggcgcagatg
     5341 attatatgca aaagcctttt tatacaaatg tattaattgc taaattacaa gcgatttatc
     5401 gacgagtgta tgaatttact gctgaagaaa aacgcacgtt aacatggcaa gatgccatta
     5461 tagatctatc aaaagatagt atacaaaaag gtgacgatac gatttttcta tccaaaacag
     5521 aaatgattat attagaaatt cttattacca aaaaaaatca aatcgtttcg agagatacaa
     5581 ttatcactgc attatgggat gatgaagcat ttgttagtga taatacgtta acagtaaatg
     5641 tgaatcgttt acgaaaaaaa ttatctgaaa taagtatgga tagtgcaatc gaaacaaaag
     5701 taggaaaagg atatatggct catgaataat ttgaaatggg tagcttattt tttgaaatct
     5761 cgcaagaact ggatattttg gatattgttt ttaaacttcc ttatgttagg tattagtcta
     5821 atcgattatg attttccaat agacagttta ttttatattg tttctttgaa tttaagttta
     5881 acaatgattt ttcttatatt gacatatttt aaagaagtaa aattatataa gcattttgac
     5941 aaagataaag aaatagaaga aattaaacat aaagatttag cggaaacgcc atttcaacgt
     6001 catacagttg attatttata tcgtcaaatc tcagctcaca aagaaaaggt tgttgagcaa
     6061 cagttacaat tgaatatgca tgaacagacc attacagaat ttgtgcacga cataaaaaca
     6121 cctgtgacag ccatgaaatt attaattgat caagaaaaaa atcaagaaag aaaacaggca
     6181 ttactatatg aatggtctcg tataaactcg atgctggata cacagctgta tattactaga
     6241 ttagaatctc aacgcaaaga tatgtatttt gattacgtgt cacttaaacg catggtcatt
     6301 gacgaaatac aattaacaag acatattagt caggttaaag gtattggttt tgatgttgac
     6361 tttaaagtgg atgattatgt ttatacagat ataaaatggt gtcgtatgat tattagacaa
     6421 attttgtcaa acgcattgaa atatagtgag aattttaata ttgaaattgg gacagaatta
     6481 aatgatcaac atgtttcgtt atatattaaa gactatggca gaggtattag taaaaaagat
     6541 atgccgcgaa tatttgaacg aggatttacg tcaacggcta acagaaatga aacgacgtct
     6601 tcaggtatgg gtttatattt agtaaatagt gtaaaggatc aattaggtat tcacctgcaa
     6661 gtcacgtcga ctgttggtaa ggggacaact gtcagattga ttttcccatt acaaaatgaa
     6721 attgttgaac gcatgtcgga agtgacaaat ttgtcatttt aaacatgcgt tttgttactt
     6781 agaattgata catcaatgca gcttcaacgt tataataaga tagatgttag tcatatgtta
     6841 aattgaaaat acaagtgcca aagcctaaag gaaatgaagt taagataaat tataggagtg
     6901 ttaaagtggc aattttagaa gtaaaacaat taacaaaaat atatggaact aaaaaaatgg
     6961 cacaagaagt gttgcgagat atcaatatgt ctattgaaga aggcgagttt attgctatta
     7021 tgggtccctc tggatctggg aaaacgacat tattaaatgt tttaagttca attgattata
     7081 tttcacaagg ttctattaca ttaaaaggaa aaaaattaga aaagctttca aacaaggaat
     7141 tatctgatat acgcaagcat gatattggtt ttatttttca agagtataat ttactgcata
     7201 cattgactgt taaagaaaac ataatgttac cactaacggt acagaagtta gataaagaac
     7261 acatgttaaa tcgttatgaa aaagtagcag aagtattaaa tatattggat attagtgata
     7321 aatacccttc tgaattgtct ggtggacaaa gacaacgaac atctgctgca agagcgttta
     7381 ttacattacc ttctatcata tttgctgacg aaccaacagg tgcactggat tctaaaagta
     7441 ctcaagattt attaaaacga ttaacaagaa tgaatgaagc atttaagtct acaattatta
     7501 tggtaacgca tgatcctgtt gcagcaagtt atgccaatag agtagtgatg ctaaaagatg
     7561 gtcaaatttt cactgaatta taccaagggg atgacgataa acataccttt ttcaaagaaa
     7621 taatacgtgt acaaagtgtt ttaggtggcg ttaattatga cctttaacga gataatattt
     7681 aaaaatttcc gtcaaaattt atcacattat gccatctatc ttttttcatt aattacgagt
     7741 gtagtattgt attttagctt tgtagcatta aaatacgcgc ataaactaaa catgacagag
     7801 tcatatccaa ttataaagga aggctcacaa gtcggaagct actttctatt tttcatcata
     7861 attgcatttt tgttatatgc caatgtgtta tttattaaac gacgaagtta tgagcttgca
     7921 ttatatcaaa cattaggttt atctaaattc aacattattt atatactaat gttcgaacaa
     7981 ttactaatat ttataattac ggcaatatta ggtattatta ttggtatttt tggttcgaaa
     8041 ctgttattaa tgattgtctt tacattatta ggaattaaag aaaaggttcc aattattttt
     8101 agtttgaggg cggtatttga aacattaatg ttaatcggtg tcgcttattt tttaacatct
     8161 gctcaaaatt ttatattagt gttcaaacaa tctatttcac agatgtcaaa gaataaccag
     8221 gttaaagaaa caaatcataa taaaattaca tttgaagagg ttgttttagg catcttaggt
     8281 atagtattga ttaccacagg atactatcta tctttgaaca ttgttcaata ttatgattct
     8341 atcggtacac ttatgtttat tttattgtca actgtgattg gggcatactt attttttaaa
     8401 agctctgttt ctctagtttt taaaatggtg aagaagttta gaaaaggtgt tataagtgta
     8461 aatgatgtca tgttctcatc atctattatg tatcgtatta agaaaaatgc tttttcactt
     8521 acggtcatgg caatcatttc agcgattact gtttcagttc tttgctttgc tgctataagt
     8581 agagcgtcct tatcaagtga aataaaatat actgcaccac acgacgttac aattaaagac
     8641 caacaaaaag ctaatcaatt agcaagtgaa ttaaacaatc aaaaaattcc tcatttttat
     8701 aattataaag aagtaattca tacgaaattg tataaagata atttatttga tgtaaaagcg
     8761 aaagaaccat acaatgtaac aattactagt gataaattta tccctaatac tgatttgaaa
     8821 cgtgggcaag ctgatttgtt tgtagcggaa ggttctatca aagatttagt gaaacataag
     8881 aagcatggta aagcagttat aggaacgaaa aaacatcatg ttaatattaa gttacgtaaa
     8941 gatattaata aaatctattt tatgacagat gttgatttag gtggaccaac gtttgtctta
     9001 aatgacaaag actatcaaga aataagaaag tatacaaagg caaagcatat cgtctctcaa
     9061 tttggattcg atttgaaaca taaaaaagat gctttagcat tagaaaaagc gaaaaataaa
     9121 gttgataaat ctattgagac aagaagtgaa gcgataagct caatatcaag tttaaccgga
     9181 atattattat ttgtaacatc atttttaggt attacattct tgattgctgt atgttgcatt
     9241 atatacatta agcaaataga tgaaaccgaa gatgagttag agaattatag tatattgaga
     9301 aagcttggat ttacacaaaa agatatggca aggggactaa agtttaaaat tatgtttaat
     9361 tttgggttac ctttagttat tgcactatca catgcatatt ttacatcatt agcatatatg
     9421 aaattaatgg gtacaacgaa tcaaataccg gttttcatag taatgggatt atacatttgt
     9481 atgtatgctg tttttgcagt gacggcttat aatcattcca agcgaacaat tagacattcc
     9541 atataaaata tacagatagc tttcagtaga gtagtggatt cggattcacg aactatactg
     9601 gaagcttttt attataaatg aagagaagtt atatttttag catgtatagt tgaatactgg
     9661 gttaaaatac catatt
//
LOCUS       DAHHRR010000094         9662 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.094, whole genome shotgun sequence.
ACCESSION   DAHHRR010000094 DAHHRR010000000
VERSION     DAHHRR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.094"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            119..1039
                     /gene="mvk"
                     /locus_tag="NBI42_001995"
     CDS             119..1039
                     /gene="mvk"
                     /locus_tag="NBI42_001995"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCD1778396.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLNGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            1044..2027
                     /gene="mvaD"
                     /locus_tag="NBI42_001996"
     CDS             1044..2027
                     /gene="mvaD"
                     /locus_tag="NBI42_001996"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCD1778397.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFMLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDLQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESDHFEDELAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            2040..3116
                     /locus_tag="NBI42_001997"
     CDS             2040..3116
                     /locus_tag="NBI42_001997"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCD1778398.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            3293..3634
                     /locus_tag="NBI42_001998"
     CDS             3293..3634
                     /locus_tag="NBI42_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCD1778399.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(3686..5008)
                     /locus_tag="NBI42_001999"
     CDS             complement(3686..5008)
                     /locus_tag="NBI42_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCD1778400.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSSKMYGGTCI
                     NIGCIPSKTLVHDGLEGKTFEASYNRKNDVVNALNNKNYHLLADDSNIDVLDFKAQFK
                     SNTEVNLLDQHGDIADSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAHAITDLENKGIAL
                     HTNVETTELSSDNYHTTVHTNVGNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAQLQTSVPHIYAAGDVKGGLQFTYISLDDYRIIKSTLYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDSRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(4995..5435)
                     /locus_tag="NBI42_002000"
     CDS             complement(4995..5435)
                     /locus_tag="NBI42_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCD1778401.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYKEKTIKDVIEDIQKEDLCHENI"
     gene            5493..5594
                     /locus_tag="NBI42_002001"
     CDS             5493..5594
                     /locus_tag="NBI42_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778402.1"
                     /translation="MCQYLCLKSVELGVFIVMIYCLKFVALTVRLHN"
     gene            6060..7457
                     /locus_tag="NBI42_002002"
     CDS             6060..7457
                     /locus_tag="NBI42_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1778403.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIQTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSIMKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGIGGTAAVGAIAAFVLSSNPVGWT
                     VLAGLAVGSGLAFAADWAYQTNKLGLKDKTDWVGHKIDESMEAVEKSADSVRNTVGEA
                     TKNIGKQFNSMKWAW"
     gene            7461..8087
                     /locus_tag="NBI42_002003"
     CDS             7461..8087
                     /locus_tag="NBI42_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCD1778404.1"
                     /translation="MLCESKQIYKNPKYRVIRYNDEYFMIDLVSTWITYFLPMINWFV
                     PKKYAKISEKEFESLNIIKPVKNNIFWPVAGSSVLFGIILRKYGNFFNVQFEKKLAIT
                     VFFIMLIGMLIFYFYLNKKLKLKIFNTNVVNKNRIVLIPTFKQGCLIVFAYIFLGSAS
                     IFTLTILLTTESQNIIIFITWVIITMFFFLVNMASIGNEKVHVIMKNN"
     gene            8211..8846
                     /locus_tag="NBI42_002004"
     CDS             8211..8846
                     /locus_tag="NBI42_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCD1778405.1"
                     /translation="MLCESRVIDKNGKYRIIKYNDEYLMIDLVSTWLTLFLPMINWLI
                     PKKYVKISKKEFDDLNIVKPTKNRAFWPAAGGSVLFGVTFRKYTQFLNILLEKNMVIV
                     ICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSI
                     MALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK"
     gene            8905..9080
                     /locus_tag="NBI42_002005"
                     /pseudo
     CDS             8905..9080
                     /locus_tag="NBI42_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020794.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9081..9662
                     /locus_tag="NBI42_002006"
                     /pseudo
     CDS             9081..9662
                     /locus_tag="NBI42_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
BASE COUNT         3423 a         1329 c         1827 g         3083 t
ORIGIN      
        1 gataaagact gcaatgaaat ttaacgcgaa tgcgtatgca acttagaata tgatgtcttt
       61 tactaataaa taaaaataat gaatcaagta taggataaat ataaaaggtg atattgacat
      121 gacaagaaaa ggatatgggg aatcgacagg taagattatt ttaataggag aacatgctgt
      181 tacatttgga gagcctgcta ttgcagtacc gtttaacgca ggtaaaatca aagttttaat
      241 agaagcctta gagagcggga actattcgtc tattaaaagc gatgtttacg atggtatgtt
      301 atatgatgcg cctgaccatc ttaagtcttt ggtgaaccgt tttgtagaat taaataatat
      361 tacagagccg ctagcagtaa cgatccaaac gaatttacca ccttcacgtg gattaggatc
      421 gagtgcagct gtcgcggttg cttttgttcg tgcgagttat gattttttag ggaaatcatt
      481 aacgaaagaa gaactcattg aaaaggctaa ttgggcagag caaattgcac atggtaaacc
      541 aagtggtatt gatacgcaaa cgattgtatc aggcaaacca gtttggttcc aaaaaggtca
      601 tgctgaaacg ttgaaaacgt taagtttaaa cggctatatg gttgttatag atactggtgt
      661 gaaaggttca acaagacaag cagtagaaga tgttcataaa ctttgtgagg accctcagta
      721 catgtcacat gtaaaacata tcggtaagtt agttttacgt gcgagtgatg tgattgaaca
      781 tcataacttt gaagccttag cggatatttt taatgaatgt catgcggatt taaaggcgtt
      841 gacagttagt catgataaaa tagaacaatt aatgaaaatt ggtaaagaaa atggtgcgat
      901 tgctggaaaa cttactggtg ctggccgtgg tggaagtatg ttattgcttg ccaaagattt
      961 accaacagcg aaaaatattg taaaagctgt agaaaaagct ggtgcagcac atacatggat
     1021 tgagaattta ggaggttaat gcgttgatta aaagtggcaa agcacgtgca catacgaata
     1081 ttgcacttat aaaatattgg ggtaaaaaag atgaagcact aatcattcca atgaataata
     1141 gcatatctgt tacattagaa aaattttaca ctgaaacgaa agtcactttt aacgatcagt
     1201 taacacagga tcaatttatg ttgaatggtg aaaaggttag tggcaaagaa ttagaaaaaa
     1261 tttcaaaata tatggatatt gtaagaaata gagctggcat cgattggtat gcagaaattg
     1321 aaagcgacaa ttttgtacca acagcagcag ggttggcttc atcagcaagc gcatatgcag
     1381 ctttagcagc agcttgtaat caagcgctag acttgcagct atcagataag gatttatcga
     1441 gattggcgcg aattggttcg ggttctgcgt cgcgtagtat ttatggtgga tttgcagaat
     1501 gggaaaaagg gtatagtgat gagacgtcat atgccgttcc acttgaatca gaccattttg
     1561 aagatgaact tgctatgata tttgttgtga ttaatcaaca ttctaaaaag gtacctagtc
     1621 gatatggtat gtcgttgaca cgaaacacat caaggtttta tcaatattgg ttagatcata
     1681 ttgatgaaga tttagctgaa gcaaaagcag cgattcaaga caaagatttt aaacgccttg
     1741 gtgaagtaat tgaagaaaat ggtttgcgta tgcatgccac gaatctagga tcaacaccgc
     1801 cgttcacata tcttgtgcaa gaaagttatg atgtaatggc gctcgttcac gaatgccgag
     1861 aagcggggta tccgtgctat tttacgatgg atgcgggtcc taatgtgaaa atacttgtag
     1921 aaaagaaaaa caagcaacag attatagata aattattaac acagtttgat aataaccaaa
     1981 ttattgatag tgacattatt gccacaggaa ttgaaataat tgagtaagga agagataaaa
     2041 tgattcaggt caaagcaccc gggaaacttt atattgctgg agaatatgct gtaacggaac
     2101 caggatataa atctgtactt attgcgttag atcgttttgt aactgctact attgaagaag
     2161 cagaccaata taaaggtacc attcattcaa aagcattaca tcataaccca gttacattta
     2221 gtagagatga agatagtatt gtcatttcag atccacatgc agcaaaacaa ttaaattatg
     2281 tggtcacagc tattgaaata tttgaacaat acgcgaaaag ttgcgatata gcgatgaagc
     2341 attttcatct gactattgat agtaatttag atgattcaaa tggtcataaa tatggattag
     2401 gttcaagtgc agcagtactt gtatcagtta taaaagtatt aaatgaattt tatgatatga
     2461 agttatctaa tttatacatt tataaactag cagtgattgc aaatatgaag ttacaaagtt
     2521 taagttcatg cggagatatt gctgtgagtg tatatagtgg atggttagcg tatagtactt
     2581 ttgatcatga atgggtcaag catcaaattg aagatactac ggttgaagaa gttttaatca
     2641 aaaactggcc tggattgcac attgaaccat tacaagcacc tgaaaatatg gaagtactta
     2701 tcggttggac tggctcacct gcatcatcac cacactttgt tagcgaagtg aaacgtttga
     2761 aatcagatcc ttcattttac ggtgacttct tagaagattc acatcgttgt gttgaaaaac
     2821 ttattcatgc ttttaaaaca aataacatta aaggtgtgca aaagatggtg cgtcagaatc
     2881 gtacaattat tcaacgtatg gataaagaag ctacagttga tatagaaact gaaaagctaa
     2941 aatatttgtg tgatattgct gaaaagtatc acggcgcatc taaaacatca ggcgctggtg
     3001 gtggagactg tggtattaca attatcaata aagatgtaga taaagaaaaa atttatgatg
     3061 aatggacaaa acatggtatt aaaccattaa aatttaatat ttatcatggg caataaatga
     3121 tttgataggg gagcaatcat ctaatttaat tagaagataa atgctccatt ttttattgtc
     3181 agccgtgtta cgtatcttga aaaaaatatt aaaaagagta aaatagatag agttaacgaa
     3241 aaaattaatg aaatcgacga tatagatatg atatagaaag gtgggtagca atatgaaaaa
     3301 tacattcctt atttgtgatg aatgtcaggc agtcaatata agaacgttac aaaagaagtt
     3361 ggaaaaatta gatcccgatg ctgaaatcgt gataggttgt caatcttatt gtggacctgg
     3421 acgccgaaaa acattcactt ttgttaataa ccgcccactg gctgcgctta ctgaagaaga
     3481 attaatcgaa aaagtttctc aacaattaaa gaaaccacgt gatcctgaag aagaagagcg
     3541 tttaagaaaa cgacatgaag aacgtaaacg tcgtaaagaa gaacaagata gaaagcttaa
     3601 agaaaaatta gaaaagcgaa aagcacaaca ataaaacctg atggcagcat cattcaatgc
     3661 gtgccaccag gtttttatgt ctcatttaga aattaaataa atcattaaat gattcggcca
     3721 tcgtaggatg tgtataaata ttatctcgta atacggtata tggaatgttt tgatcaatcg
     3781 caagtttaat tatattaatt aattcttcag attgcttacc atataatgta gcacctaaaa
     3841 tcatattatt ttcattatta atgactactt taaataaacc ccttgaatca ttgttaattt
     3901 tgtgacgagg tattgcactt actaaaagtt ggtgttcagt gtaatcataa tgttgcgcgg
     3961 cagcttcttt actagttaat ccaacacgag ataatggtgg atctataaat actgtataag
     4021 gcacgctgcc tctattgtca gtcgtacgtg actgattacc atataaagtt gatttgataa
     4081 ttcgataatc atctaaagat atatacgtaa attgaagtcc gcctttaaca tcacctgcag
     4141 cataaatatg tggcacagat gtttgaagtt gagcattgac tttaatttcg cctctatcgc
     4201 ctaattcgat atcagtattt tctaaagcta aatccgtatt cggtttgcgc ccgatagcca
     4261 aaagtactgc atcagcctca aagttaccaa cgttagtatg gacagttgta tgataattgt
     4321 cagatgacaa ttcagtcgtt tcaacatttg tatgcaatgc aatgccttta ttttctaggt
     4381 cagtaatagc atgtgcaacg acatcttgat cttcgcgtgg cataaatgat tcaccacgtt
     4441 ctaatactgt aaccttacta cctaaattcg caaacattga agcaaattct aaggcgatat
     4501 aaccgccacc tacaataacg agatgcttag gttgatagct aatgtttaat aaacctgttg
     4561 aatcgaagac atgtttagct tgatcaaggc ctttaatgtt aggaatgaca gaggtagcac
     4621 cagtattaat aatgatatga ggtgcagtaa tactatcggc gatatcgcca tgttgatcta
     4681 ataaattcac ttcagtatta gatttaaact gcgctttaaa atccagtaca tcaatgttac
     4741 tgtcgtctgc taataagtgg taatttttat tgtttagcgc attgacaaca tcgtttttac
     4801 ggttataact tgcttcaaag gttttgcctt ctaatccatc atgtacaagt gttttcgaag
     4861 gtatacatcc tatattgata caagttccac catacatttt cgaagattgt tcgataactg
     4921 cgacgtgttg acctgttgat gcagcgtatt tcgctaaagt tttaccagct ttcccaaatc
     4981 ctattacaat taaatcatat gttttcatga cataaatcct ccttttgaat gtcttcaatg
     5041 acatctttga ttgttttttc tttataaaaa ttaataatga tattctgttc gtcttgttga
     5101 taatgtgaca tggtagttgc aatattacga gcaatttgac agtgactgcc ttcgtcgcca
     5161 gtaaatagac gtgtgtgatg ttctttctct aagacaaaat gtttatataa tgttgctaga
     5221 gagacatctg cactttgatc atttgctaaa taaccgccat ctttacctcg tattgtgtca
     5281 atcattttta aatcgacaag ttgagtcgtc acgcgtcgta attgaacagg atttaaacaa
     5341 gttaattctg ctaatgaact actattgaat ttttctgaat gatgcttagt taaaaaagct
     5401 aatacatgca cggcaatgtt aaattctaaa ttcaatgttt ctcacctcgt aattgtaact
     5461 gtattagtta caattaaaaa tcattttgct caatgtgtca atacttatgt ttgaaaagtg
     5521 ttgaactagg agtgtttata gtgatgattt attgtttgaa atttgttgca ttaacggtga
     5581 gattgcacaa ttgaaatttt agttatgtat gaactgatag atatgaaatg aatattgaaa
     5641 atgagggcaa taaaattttg aatgatgatg ttgtgtgatt ttgtaaactt gttcgtaatt
     5701 tagagacgtt ttgatatttc gcatgatgag gaagtacttt taacaactag tgacatattt
     5761 aaatcgtaaa tatattttga gatattaata tattttaggt taaggatgtt gtcgcaataa
     5821 aattaaaata ttgaaagata atttttattg attaattgcg aatatacaaa gacaccatta
     5881 tatccatatg ctaaaataag aaaaaaggcg acatgttatc tcaatttacc tgcatctaga
     5941 cccctttaca taagaaataa tcatcgccga gttttaaaat tttacaactt tatagatgta
     6001 aaaagcattt gattttaaat agtttgaaaa tcatatattt ttgaaaaagg agcatgccga
     6061 tgagtattga catgtattta gacagatctc gaaaccaagc ttcaagtgtg gggaatttga
     6121 gtcaaacaat gaattcaaat tatgatgcgt tggaaaaagc aattactcaa tttatcaatg
     6181 atgatgcgct taaagggaaa gcgtatacgt cagctaagca attttttagt acggtgttaa
     6241 ttccattatc aacaagtatg aaaacattga gtgatttaac gaagcaagct tgcgataatt
     6301 ttgtgtcacg ttatacgagt gaggttgata gcatatcttt aaaagaatca gagcttgaag
     6361 aagatatcag atcattaagt caacaaatta cgcgatatga aaatttgaat aacaatttga
     6421 aaaagcatgc ttccgataat cagcaagcca tttcatcgaa ccaacaaata atacaaacat
     6481 taggtcaaca aaaacatgaa ttagaagaga agctacgcaa attgcgtgag tttaatcaaa
     6541 aatcaccaga aatatttaaa gaagttgaag aatttcaaaa aattgtccaa caaggactta
     6601 cacaagcaca aaatttttgg aacttttcaa caaatcaatt taatattcct tcaggtaaag
     6661 aacttgattg ggccaaagca agccatgaaa aatatttgaa agttgccatg gggaaaattg
     6721 aacataaagc agagaaagaa actttaaata aagcagactt tgctgttata aaggcatatg
     6781 ccaaagagca tccggaagac gatatcccga aaagtataat gaaatatata aatgacaata
     6841 aagatagtat taaacgagat ataggattag atatcacatc aacactttta gagcaaggcg
     6901 gtataaatgc aagtaaattc ggtgtattta tcaatacagc aggtggagtg aaaggcccag
     6961 ccggtccaaa ttcatttgtg gaagtcaaac gtacatcagg taatgtgttt atagaaaatg
     7021 gtagtaaatt tgctaaaggt ggaaaatatc taggtaaagg tgttgcaggt gtaggatttg
     7081 gtataggtat gtatgatgac cttgcaaatg atgataaaac atttggagag gcgttgtcgc
     7141 ataatggtat gacgttagca gctggaatag gtggaacagc tgcagtgggt gcaatagcgg
     7201 cttttgtctt aagtagtaat ccagtaggat ggacagtttt agctggatta gctgtgggtt
     7261 ctggattagc atttgctgca gattgggctt accaaacaaa taaacttgga ttaaaagata
     7321 aaacagattg ggttggtcac aaaatagatg aaagtatgga agccgtagaa aagtctgcag
     7381 atagtgttag aaatacagtt ggagaagcta caaaaaatat cggcaaacaa tttaattcaa
     7441 tgaaatgggc gtggtaaaca atgctttgtg aatctaaaca aatttataaa aatcctaaat
     7501 atcgtgttat tagatataat gatgaatatt tcatgatcga tttagtaagt acttggatta
     7561 cttatttttt gcccatgatt aattggtttg ttcctaaaaa gtatgcaaaa attagcgaaa
     7621 aagaatttga aagtttaaat attatcaaac ctgttaaaaa caatattttt tggccggttg
     7681 caggaagttc agttctattc ggaattatat tgagaaagta cggcaacttc tttaatgttc
     7741 aatttgaaaa aaaattagca atcacggtat tttttatcat gttaataggg atgttgattt
     7801 tttatttcta tctaaataaa aaattaaaat taaaaatttt taacaccaac gtggttaata
     7861 agaatagaat tgtattaata ccgactttca aacaagggtg tttaatagtt tttgcgtaca
     7921 tttttttagg aagcgcttca atatttactt taaccattct tttgacgaca gagtcacaaa
     7981 atataataat atttataact tgggttatta tcacgatgtt tttcttttta gtgaatatgg
     8041 cttcgatagg taatgaaaaa gttcacgtta taatgaaaaa taattgatta catttaaaat
     8101 attctaaatg ttttcgacac aatcctttta agacgctagt agaattcaaa tgacttctaa
     8161 tgtatatgaa agtgtatcaa tataaaacca atggaaaaga agtggagaca ttgctttgtg
     8221 agtctagagt catagataaa aatggaaaat atagaattat aaaatacaat gatgaatatt
     8281 tgatgatcga tttagtaagt acctggttaa ctttatttct tcctatgatt aattggttaa
     8341 tccctaaaaa atatgtcaaa atcagtaaaa aagaatttga cgatttaaac attgttaaac
     8401 ctactaaaaa tagagctttt tggccagctg caggtggctc agttttattt ggagttacat
     8461 tcaggaaata tactcaattt cttaatattc tattagaaaa aaacatggtg attgtaatat
     8521 gttgtgcaat atttctgggt gttttaatac tttttttatt tctgaatcgt aagctaagat
     8581 tggaaattta taataataac tctagtaaag ggaaaataat tttatttcct tcattaaaaa
     8641 acttttgctt cacaatattt tattattttt tatttggcgg tctttcaata atggctctaa
     8701 gtatgttatt aactttaaat cctcaaaata taataggctt tattggttgg ttggtaatga
     8761 ctgcaggttt ctttctgtta aatatgtcat cgattattga caaaaaaatt tatgtattat
     8821 ctaaaactaa cacggtggaa aaatgatggt ttagctggat ttactgcggg ttctgtttcg
     8881 tcaatacttg tatattggac caatcaaaga aatgaacttg gaataaaagg taaaaacgat
     8941 tggataggac ataaactaga cgctggtata gatgctgtag aaaaatctgc tgaaaaaaca
     9001 gtagatggtg ttgaaaatgt catggtgaag ctacaaaaag tatttctaat catataagcc
     9061 ctatgaaatg gagctggtaa atgttgctgt gcgaatctaa aatcatcaat aaaaatccta
     9121 aatatagaat tattaaatat aatgatgagt atttaatggt cgatataata agcacttgga
     9181 ttagtttgtt ttttccattt attaattggt tcatcccaaa aagatatgtc aaaatcagtg
     9241 aaaaagaatt tgacgattta aatattgtta aacctgctaa aaaaatgttt tttggccagt
     9301 tgcaggtagc tctgctttgc tgggagttgc attaagaaaa tatacacatt tacttgacgt
     9361 tcaacttgat aaaaaattag ttattgccat atgttgcatc acatttatag ggattttaat
     9421 attttatgta cgcctaatta aaaaatcatc tttaaatatt tataatacta aaaataaaag
     9481 gtcaaaaatt tttttaatac ctacactaaa aaatgtttgt ttcacattat ttggatatat
     9541 tttatttggc ggattgacta tgctattcct agatgcacta ttatcaatga gttaccaaaa
     9601 cataatagta tattttgctt ggattgcagt tataatgggt ttttttctag tgaatatagc
     9661 tt
//
LOCUS       DAHHRR010000095         9574 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.095, whole genome shotgun sequence.
ACCESSION   DAHHRR010000095 DAHHRR010000000
VERSION     DAHHRR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.095"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            73..465
                     /locus_tag="NBI42_002007"
     CDS             73..465
                     /locus_tag="NBI42_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778406.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKDNYNKYIQGQKQRVIFENGHFV"
     gene            complement(594..1970)
                     /locus_tag="NBI42_002008"
     CDS             complement(594..1970)
                     /locus_tag="NBI42_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1778407.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMVASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGDHLRRAISYLMISTVGGIGLAGVIGGLIAT
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQPSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSFICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTSLQPSQYIIAVVIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            2204..3196
                     /locus_tag="NBI42_002009"
     CDS             2204..3196
                     /locus_tag="NBI42_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCD1778408.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWTSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHSVEVSFKEGE
                     VVIKDNPIGVLTNHPDLDWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNEIVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            3522..4472
                     /gene="lytM"
                     /locus_tag="NBI42_002010"
     CDS             3522..4472
                     /gene="lytM"
                     /locus_tag="NBI42_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HCD1778409.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHMHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(4524..5183)
                     /locus_tag="NBI42_002011"
     CDS             complement(4524..5183)
                     /locus_tag="NBI42_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1778410.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWAYTY
                     Y"
     gene            complement(5197..6117)
                     /locus_tag="NBI42_002012"
     CDS             complement(5197..6117)
                     /locus_tag="NBI42_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1778411.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVISLVLIGMGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVAEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            complement(6114..7280)
                     /locus_tag="NBI42_002013"
     CDS             complement(6114..7280)
                     /locus_tag="NBI42_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1778412.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKQQKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLATVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRALITWFYTMLWSIVGVVWIMFSIPNTFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            complement(7347..8870)
                     /locus_tag="NBI42_002014"
     CDS             complement(7347..8870)
                     /locus_tag="NBI42_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778413.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFSNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FDKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            9141..>9574
                     /locus_tag="NBI42_002015"
     CDS             9141..>9574
                     /locus_tag="NBI42_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCD1778414.1"
                     /translation="MELKVDDFVKNIKRPYLTVLGVFVVAYSLFFDSLMFFYGKLYDK
                     LPVYLLVFMAFTAVILIMMYIQEKNKNYKVEKRYVVRYLTLNVIVGYTLPLLLASIYV
                     FGVAGFGFNVFNYWLGIVMMLFISWLGLFLFYKNKFDSENPN"
BASE COUNT         3220 a         1454 c         1647 g         3253 t
ORIGIN      
        1 tgtatattga actatgtgat tgaaaaaaat tttaattaag gcttgttaaa aattaagagg
       61 ggatgttttt aaatgcaatt caaattaaaa gaagaagaga ttattagttt tttagaattg
      121 aaatatccag aaaaagagtt cgaatatggt cgtttgttag ttggacaaca taaacgtgat
      181 gatttagatg tttattactt tggtgatacg tttttaatgt gcacgattat ttcattcaag
      241 acatttgaaa ttaaagaaac agtagaatta tcatatgatg ctgttaatcg tattgtgtta
      301 aaagatggat ggttatttag aaaaatgaga atagaaacaa tgcaaaaagt gttaaaatac
      361 ggtacatcta aattaatgtt aactgatttt caaaaggaca attataataa atatattcaa
      421 ggtcagaaac aacgcgtgat atttgaaaat ggccattttg tctaattgat agtgaataat
      481 aattagagta agcgaagaac aataaaaaat gttcttcgct tatttttata gaatttgatg
      541 tatatcattt taaagtgata cagatatttc gaaacatgat tcttctatta tatttaagat
      601 ttaactgttt ttggaatgat catgtatgca gacaatgagc cgagtatcat taatacaatg
      661 ctaacgataa atgttacagt tgcagctaca cttggtgcat agtttagttg taacatactg
      721 aaaactgtag tacttagtgc tataccaaag gcgccaccta gtgtgccact cattttatat
      781 aatcctgtag ctaaaccaac tttttcatta ggcatactga aaattgcaat agtaaggccg
      841 ggtgttgcga ctaaaccatt tccgattgcg catatgacaa acccaataac aacagcaatg
      901 atatattgtg atggttgaag tgatgtcatg ctaataatag tgatgccgat gacaggaaac
      961 aacggaccaa tgatgagcat caacttgcca ccgaagcgta atgtcgcttt ttcgcctaaa
     1021 cgaatcatcg caactgccac aatggcatat ggcaatgtaa caagtccaga ttgcgcagct
     1081 gataaaccaa ggtgtgtttg agcatatatg aaaaagacca ctgttacgcc tagaccacta
     1141 tttaaaacaa agttatttaa aaatgcacca atgaacggac ggttgcgtaa tactgagaaa
     1201 tcaataaaag gtacttcatg tcgacgttcg atgatgatga atatcaacgt agtgatgata
     1261 aaaatgctca gacaaatgaa tgaaaatgta ctaaaccaac cttgttcgaa tccttgtgtt
     1321 aacaataatg taaagctacc aatcataaca gcgaaaatcg acataccttt gtaatcgaat
     1381 ggatgacgat ggctaggttg acttactttt tcaggtgtgc cttttagaag caatatggca
     1441 atgaaagcaa tgactatact aatgatgaaa ttcgtttgcc atccgaaatt tgtggcgatt
     1501 aaaccgccga taacaccagc taggccgatg ccaccaacag tactaatcat tagataacta
     1561 atcgctcgtc ttaaatgatc tcctttaaat tgattattta gcacgccaac tgttgaaggt
     1621 aacaagatag ctgctgatag accttgtaaa attctaccga tgatgagcag tgcagtgatg
     1681 tccgatataa ttaatagaag agatgctacc atactgatta tgagacccat gtatgtcatt
     1741 ctcaattgtc ctattttatc agcaatatca cctgcagcca ccatgaagat acctgtggcg
     1801 aaggaagtta aactaataga taaatttaac acggcaggag aggtttgata tgtttgacca
     1861 acgagaggtc ctatattaat aaatgattgt gcaaacaacc aatatgttaa tgcagacaac
     1921 ataatcgcaa ttataatatt actgcgtggt gaagattgtg tgttattcac agatgtcact
     1981 ccttgtaaaa attaaatata gaaagtcatg agatgaagtg aacggatgta taaatgatcg
     2041 ttcactcaca cattagtcat gatttcctta catatcatac cacagttatt gagaatgaat
     2101 atcaattaat tgcttgtatg attgattttt tatagtatgt ttaagtgaaa cagctttatt
     2161 tgaaagtgat taaagtaaat gaaggtacag aggagtgagg acaatgtgca caggattcac
     2221 aatacaaact ttaaataatc aagtacttct tgggcgcaca atggattatg attatccatt
     2281 agatggttcg ccagcagtga cgcctagaaa ttatcgttgg acatcgcgca ctggcacgac
     2341 aggccaaacg caatatggct ttattggtac aggaacagat atggaaggtt ttatttatgg
     2401 tgatggtgtt aatgaacatg gcgttgccat ttcaacacaa tatttccgag gttatagttc
     2461 atatggatca acacacaaag cggacgcgat gaatattacg caaaatgaaa ttgtgacatg
     2521 gattttggga tatactacaa gcattgaaga tatgaaacaa caagcatccc aaatacatgt
     2581 tgtagctgta tatttaaatg atatcggtga agttccgcca ttgcattatc atgtttccga
     2641 tgcaactgga cattcagtcg aagtttcatt taaagagggt gaagtggtta taaaagataa
     2701 tcctattggt gtcttaacaa atcatccaga cttagattgg cattatagta atttaagaca
     2761 atatatcaat atttctcctt atccagcaac agcaaattta ttggaaggtg taacgattga
     2821 acctttaggc aatgaagcag gtacatttgg attgccaggt ggatttactt caactgagcg
     2881 ctttgtgaga atggcattta tgaaagcaaa tattgctcaa aacaatgata aagaaatgga
     2941 tttaatgaat gcattttatt tattagatgc ggtaaatata ccgattggaa ttgtacgtcc
     3001 gcatgatgct gacaatcact atacgatgta tcagaccgta ataaatttaa ctacaagaac
     3061 gttatatatt aagtattatg gcagcaatga aatagtagca ttaaagctca cagatgattt
     3121 aattaataga aaagatatga cgatttttaa gcctgagaag catatcacta ttagaaagtt
     3181 gaatgacaat caatagcgat tgataatgga atttggttga tatgatttga tggatgatta
     3241 ctatcataca ttagcgttga accgcgaaca tggacgaatg attagttgtt aattttgaaa
     3301 atttataaaa ggttaaacga atgcagtgag agatgtcatt ttaatatcaa tgtatggcta
     3361 ttttgtaatg acaatgtaat gagtttagta aaacatttcg ggaatattaa atagttggaa
     3421 aaagagaatt aaatccttta ctcagttgtc taattctttt agtatgtgca gtacagttta
     3481 attgaaaact aactttaact ttaatggagg atgttttata catgaaaaaa ttaacagcag
     3541 cagcgattgc aacgatgggc ttcgctacat ttacaatggc gcatcaagca gatgcagcag
     3601 aaacgacaaa cacccaacaa gcacatacac aaatgtcaac acaatcacaa gacgtatctt
     3661 atggtactta ttatacaatt gattctaatg gggattatca tcacacacct gatggtaact
     3721 ggaatcaagc aatgtttgat aataaagaat atagctatac attcgtagat gcacaaggac
     3781 atatgcatta tttttataac tgttatccaa aaaatgcaaa tgccaatgga agcggtcaaa
     3841 catatgtgaa tccagcaaca gcaggagata acaatgacta cacagcgagt caaagccaac
     3901 aacacattaa tcaatatggt tatcaatcaa atgtaggtcc agacgcgagc tattattcac
     3961 atagtaacaa caaccaagcg tataacagcc atgatggtaa tggaaaggtc aattacccta
     4021 atggcacgtc taatcaaaat ggcggatcag caagtaaagc gacagctagt ggtcatgcga
     4081 aagacgcaag ctggttaaca agtcgtaaac aactacaacc atatggacaa tatcacggtg
     4141 gtggtgcgca ttacggtgtc gactatgcaa tgcctgaaaa ttcaccagtt tactcattaa
     4201 ctgatggtac agtagtacaa gcaggttgga gtaactatgg tggcggcaat caagtaacga
     4261 ttaaagaagc gaacagtaat aactaccaat ggtatatgca taataatcgt ttaactgttt
     4321 cagctggtga taaagtcaaa gctggtgacc aaattgcata ttcaggtagt acgggtaatt
     4381 caacagcgcc tcacgtacac ttccaacgta tgtctggtgg catcggtaat caatatgcag
     4441 tagacccaac gtcatacttg caaagtagat aatacagtaa atcccaagtt acgatatcat
     4501 acgcaacttg ggattttttc gttttaataa taggtataag cccattgctt gtcctctaaa
     4561 acatgttgat aaaaaggatc ttggccaatt aacttgatat catctgcaag tgcttccact
     4621 tcatctaaat gatgggtagt taatataatt aaacatttag atttcataat gttaagtagt
     4681 tggtggatgt catgtctaga tttcaaatca ataccaactg tcggttcatc taaaatgaga
     4741 attcgaggtt gacctagtaa acctactaat atattaattt tacgtttatt cccacctgac
     4801 aatgtagata ctttggcaga cgtatcatca aagtttaatt gctgtaaata ttcgttgata
     4861 gttgtatcgt taattggatt tttacaaagt gatttaaaaa atttaatgtt ttcagccact
     4921 gtcatgtgtt caaataacgc aatgtcttgt ggcacataac cgatgtgatt ttgtatttgt
     4981 ctttgattcc atttttcgcc gaaatagttg atagttccat cattagcttt ttcaatacca
     5041 gcaatcatac gaagtaatgt tgattttcca gcgccattat caccaagtaa tacggttaag
     5101 cgattactat caaaggacat agttaaatga ttgaaaatct gtttgttacg gtaacgcttt
     5161 gaaaggttat taatttctat catcagttac gctcctttac atgaaaataa tcaagtatac
     5221 gatacccata gcaagtgcat atataaatgt catgaataat cgatgactta ttgtttgaat
     5281 atggaataag ataaagacga tacctatctc ataaatcaat ataagtaaca gtgattttaa
     5341 gtaaaatatt aagctgagtg gttgagacaa atatagacta actgccaata gtaccaacaa
     5401 taacaaaatc gtatgtgtca ttacataagt actatatagt ttgaaacggc ttaaatgata
     5461 ttgtgataat cgttgcaatg ctgcttgttg gtttaaacga taatgaagta ctacttgaac
     5521 agcgctaaca aataaaatca ccgcaaagat taagctaatt gaaatagagt gttgtgcttg
     5581 tttagtaagc gacacaaatt tgattttaga ttcaggtgta tgtttatgat aggacttgtt
     5641 gatagcatcg atggattgat gctgtttcat atcctcaagg tgttcgtaaa taatgttagg
     5701 aatttgctgt tcatataatg aactactaac aatttctaca gcaataccac ctataaagtc
     5761 atctctacca tataactgta tcgtttcttt taaacggttc tcttttaatt tttgagagaa
     5821 acctttagga atttgcatac ttaaaatagc ttccttttta gtaacatcat cttcaatata
     5881 gctttcatct tcagcgactt ttttaatagt tacatagtca gattgtttaa ttttattgac
     5941 gaatgatttt gatgcagtgg tttggtctaa atcttgaatg gtaatcggta ttttgaagtt
     6001 gtcatgtgct acacggtaac ccataccaat aagtacgagt gagatgacaa tggttgttac
     6061 gagcaagatg tattgtaacc attgcttgaa cacaacaagt tgtatataag gcttcattga
     6121 cgatacctcc aaaccaatac agctaaatta attatcaaaa gtgcgatgaa gctaagatag
     6181 aaactagggt gcagttctaa aatgtagttg tttaaaataa tttctaacaa ttgatttgtt
     6241 acaactgcga atggttgaat attgaaaaca ccatttgcta tatgttgtaa aaaaatcgta
     6301 ggtattgtta aaccagataa caccaggatg acaatagcta atatgacttt actaatacta
     6361 ttcaacaagc ctgttgttaa aagttcgata agtaataacc acagtattaa aaaggtaaca
     6421 taatagctta aatgaatggc taacgttggc caattatata attcaaaggt attcggaata
     6481 ctgaacataa tccaaactac accaacaata ctccataaca tagtataaaa ccatgtaatc
     6541 aacgcacgaa tgattaacaa acgctcttta gaaaaatgaa acattttcaa tcgcgctttc
     6601 aatacagtat cttgattcat tttcaaaact gtaaataaag atagtgcaaa gatgaatacc
     6661 gttgctaaaa atcctgtaat tgcataataa ctgcccgtat cgtataaatg aatcggttct
     6721 aagttaaatg cacctgaacg gtttaatcct gtaatcagca aatcagtcat aacattgata
     6781 ctgtcagaat gtgatgcttt cggtgctaag tcttgaaaag ctaagatgcc acccattgat
     6841 cgcataagac gttggtaaac agaatctgtt agctgagata ggacgacact tttcatggat
     6901 tgttgatcat atgtatatac tgaaattggt agttcgcctt gtttataaaa tgccttggtc
     6961 atacctttat caaaaacaaa atagccttga agtttctgct gttttagcaa agtatgtgct
     7021 tgcttatcat catatgcttt aatgctcaca tttttaccta ggttactccc tttaccaata
     7081 gagtttaaga ttaatttcgt ttcacttgat tggtctttat ctacgacgcc tatattaaaa
     7141 tgattgtcat cttctgttac atgttggatt gtcgttaatg taataaggag tgccgctaat
     7201 ataaatagta aatagataat caaataccac tttttcaata aaaaagagtg gtagatgcga
     7261 aacaaatgta ttgttttcat ttaaccactc ctccaatgat aagattgaaa ggcaagatga
     7321 ccttccaatc ttattttata ttttagttat ttagatgcct tttttaaaat tgattcaaac
     7381 attttgccgc catttttttc gatttctttt tcaagttttt cacggtcttt tgaagataga
     7441 ctattgaaat ctttcgcacc actatcatca aaatcaatat ctgctttcaa ttttgtgcta
     7501 gattttaaaa tgaaattaat tggttcttca gcatatttga tgccgatatt taacgtagat
     7561 ttctgagtgt tattttttac gtcagaatct atattatttt caaaagtgaa ttcattttcg
     7621 tcgctatatt tatctaatgc gacagtgatt ttacctttat cttgacgttt tgtgccatct
     7681 actttttctt ggttatctaa tttgattttt gattcatcat attctgtctt tttaccaaat
     7741 tcgtatttat cattatattt attatctttt tctttagaag atacgccttt aattgtatat
     7801 ttcgcttcag catacgtgta tttatcttga tcgaaatcaa gtgcgtaatc tagttttaac
     7861 ttatcgtctt ctaaagtatt agtacctttg attttagttt tattattttc tttgtctgta
     7921 atagtaattt ctcgttttac aatcgtatgt ttttcggtat aaattttaga ttgaatttta
     7981 gcaaattcat cctttttagt ttctttgaca tcgtcaattg cttttttgat gtccttatca
     8041 aagtcttttg tagcaccttg ttcttccatt aattttttaa ggtctttatc ttttttagct
     8101 tcttctaata cagctaatgt aattttttta gtgtcagctc tgctaagtgt taacgtgaca
     8161 ggtctaactt tgtacttttc accattaacc ttaatttctt cttttttacc tttatcaaaa
     8221 ttatcgtcat ctaatttgtc gacaataagt tcggaatatt tttcggcaat tttgctgtag
     8281 tcactttgtt gtgcttgagc attactaaaa agagtattta aatttagttg ttggtttgta
     8341 ataccatttt cttttgctgt ttcttcatct tcacctgtaa gttttgaata agttgataat
     8401 aaatcagaat tattaacact atatttccct ttaaataatg gtgattcgaa ataatgctta
     8461 tctttatctg cagctaactg gaatttccct aatgcagagt ctgctattgt tggttcaaga
     8521 ttaatcattg atttctcttt tttaggatca tgtccatatg acattttaat tttagaagca
     8581 ttaacaacag atttaggaat accaagcccc ttaacaattt catctgatgc atctgcgctt
     8641 aattctaatg aagataaaaa tgaattatct ttcatctttt cttggaactt cacttcgttc
     8701 tcaaaacggt cattaaaata atctttatac atttttgcag tttgttgttc gctttttaag
     8761 tatgtatttt tcggtgtgtt tgcaaaaaat gcataaactc cccatgcgat tccacctatt
     8821 aataataaga caataataat aggaattata atttttaact ttttagacat tttgcttcct
     8881 cccaattatt tatgagttaa tcatatcaga acaatacatt ttattaaatg gtttatttta
     8941 aattttgttt aaattaatag aattttagtt ataataatgt ttaataagtt attgaaaatc
     9001 taataaacta caaaaaatag tttgattaca taatgattct tgaaaaatgt tggttaactt
     9061 aataacatgc atttttttgg caaagaagat ttatttaact tataaaaata ttgaagtaag
     9121 attggggaga ttatgaattt atggaattga aagtcgatga ttttgtaaag aatataaaaa
     9181 gaccgtactt aactgtattg ggagtatttg tagttgccta ttcattattt tttgattcat
     9241 tgatgttttt ctatggcaaa ctatatgata aattgcctgt atatttgctg gtgtttatgg
     9301 cctttacagc tgtaattttg attatgatgt acatacaaga gaaaaataag aattacaaag
     9361 ttgaaaaaag atatgtggtt agatatctca cacttaacgt tattgtggga tatactttgc
     9421 cattgctttt ggcatctatt tatgtttttg gggtagctgg ttttggattt aacgttttta
     9481 attattggct aggtatagtg atgatgctat ttatttcttg gttaggctta tttttatttt
     9541 ataaaaataa atttgatagt gaaaatccga ataa
//
LOCUS       DAHHRR010000096         9559 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.096, whole genome shotgun sequence.
ACCESSION   DAHHRR010000096 DAHHRR010000000
VERSION     DAHHRR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.096"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..150
                     /locus_tag="NBI42_002016"
     CDS             <1..150
                     /locus_tag="NBI42_002016"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HCD1778415.1"
                     /translation="TVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLE
                     ADSDY"
     gene            178..1356
                     /gene="deoB"
                     /locus_tag="NBI42_002017"
     CDS             178..1356
                     /gene="deoB"
                     /locus_tag="NBI42_002017"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCD1778416.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(1487..2302)
                     /gene="phnE"
                     /locus_tag="NBI42_002018"
     CDS             complement(1487..2302)
                     /gene="phnE"
                     /locus_tag="NBI42_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCD1778417.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIIPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(2299..3099)
                     /gene="phnE"
                     /locus_tag="NBI42_002019"
     CDS             complement(2299..3099)
                     /gene="phnE"
                     /locus_tag="NBI42_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCD1778418.1"
                     /translation="MPLETPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            complement(3101..3874)
                     /gene="phnC"
                     /locus_tag="NBI42_002020"
     CDS             complement(3101..3874)
                     /gene="phnC"
                     /locus_tag="NBI42_002020"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCD1778419.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFTVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            complement(4088..5044)
                     /locus_tag="NBI42_002021"
     CDS             complement(4088..5044)
                     /locus_tag="NBI42_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1778420.1"
                     /translation="MKKFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFVD
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            5273..6772
                     /locus_tag="NBI42_002022"
     CDS             5273..6772
                     /locus_tag="NBI42_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1778421.1"
                     /translation="MKKIYKSLTVSAIVATVSLSVLPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVNYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAAFIGDSSLVEDSS
                     PKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSLKASGLTLDTKTKLLD
                     FERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADPQPNTPDDHTPPNQNE
                     KVSFDIPQNVSVNEPFEVTIHLKGFEANQTLENLRVGIYKEGGRQIGQFSSKDNDYNP
                     PGYSTLPTVKADENGNATIKVNAKVLESMEGSKIRLKLGDKTLITTDFK"
     gene            6823..8358
                     /locus_tag="NBI42_002023"
     CDS             6823..8358
                     /locus_tag="NBI42_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCD1778422.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRK
                     QYDQSFKIDNGDFLQGSAFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLQY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVQYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFDTLQQHLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKEIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGKVVLSTDEYENVSVESCELL
                     PVIDDPTFTIDEDDQHIRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSGADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGLSYTIHVGRPKGQRVSDMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            8513..8818
                     /locus_tag="NBI42_002024"
     CDS             8513..8818
                     /locus_tag="NBI42_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778423.1"
                     /translation="METIKQEVRESIVKGIIAGYKDYVNERNEVKKRMVISDAYVYTK
                     SNHIENQVAKHLENFVKYIKENAGPSWKYLKFIFNKDEEKNNIMFILKNEDYFDEKK"
     gene            8881..9279
                     /locus_tag="NBI42_002025"
     CDS             8881..9279
                     /locus_tag="NBI42_002025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778424.1"
                     /translation="MAKNTDINFDTVEEDFEIRHQMTADSILFNIKEGSNRDINSYFL
                     IITYRIDKESKQLAAIKQWLPNPETNSAIMVDDLTPLLDKVIVEREDYHIDEEELEVL
                     KNDGELELIDVEHAFGISIDEGNDMIESER"
     gene            9285..>9559
                     /locus_tag="NBI42_002026"
     CDS             9285..>9559
                     /locus_tag="NBI42_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1778425.1"
                     /translation="MFIGKNLEYVRKLNALSRKELSEKINVSEQAIWQYETKNMMPEI
                     SKIYDMTSIFNVKSSYFISEQPEELLINSVDKHSIAFRAKNYKVSTKL"
BASE COUNT         3282 a         1500 c         1768 g         3009 t
ORIGIN      
        1 acagtaggtg ctgatgtaga agtaaaagca tcaggtggcg tacgtaattt agaagatttc
       61 aataaaatgg ttgaagcagg tgcgacacgt attggtgcga gcgcaggcgt tcaaattatg
      121 caaggtttag aagcagattc agattactaa tatatataaa atttgggagt gatagctatg
      181 acaagaccat ttaatcgtgt acatttaatc gtaatggatt cagtaggtat tggtgaagcg
      241 ccagacgcag ctgattttaa agatgaaggt tcacatactt taagacatac cttagaaggc
      301 ttcgatcaaa ctttaccaaa ccttgaaaag ttaggtctag ggaacatcga taaattacca
      361 gtagtaaatg cagttgaaca accagaagca tactatacta aattgagtga agcttcagtt
      421 ggtaaagata caatgactgg tcactgggaa attatgggat taaatattat gcagcctttt
      481 aaagtatacc ctaatggatt ccctgaagag ttaattcaac aaattgaaga aatgacaggt
      541 cgtaaagttg ttgctaacaa accggcatcg ggtacgcaaa ttatcgatga gtggggcgag
      601 caccaaatga aaactggtga cttaattgtt tatacaagtg cagacccagt attgcaaatt
      661 gctgcacatg aagacattat cccattagaa gagttatatg atatttgtga aaaggttcgt
      721 gagttgacaa aagaccctaa atatttaatt ggtcgtatta tcgcacgtcc atatgttggt
      781 gaaccaggaa actttacacg tacatctaat cgacatgact atgcgttaaa accttttggt
      841 aaaactgtct tagatcattt gaaagacggt ggttatgatg ttattgccat cggtaaaatt
      901 aatgacattt atgatggtga aggtgtaaca gaagcggttc gtacgaagag taacatggac
      961 ggtatggatc aattgatgaa aattgttaag aaagatttca caggtattag cttcttaaac
     1021 ttagtagact ttgatgcatt atacggtcat cgtcgtgata aaccgggtta tgcacaagca
     1081 attaaagatt tcgatgatcg cttgccagaa ctgtttagca acttaaaaga agacgattta
     1141 gtaattatta cagcagacca tggtaatgat ccgacagcgc caggtacgga ccatacgaga
     1201 gaatatatcc cagtaattat gtacagtccg aaatttaaag gtggtcatgc actagaaagt
     1261 gatactacat tcagttctat cggtgcaact atagcagata atttcaacgt aacattacca
     1321 gagttcggta aaagttattt aaaggaattg aaatagaata aatttagata taataaaaac
     1381 agcagtgaag ttaactataa caatagtttt cctcactgct gtttttatta taatagagaa
     1441 acgtaagacg gtaggacctc ttatttagga gtatcctgat ttaatgttaa acaatacgtt
     1501 ttcggattga accggaaatt aaatcgacaa ttgcgaccat tagtactaaa ccgattaata
     1561 taatacctac acggtcccaa gaacgtgttt gaatggcaaa tatgagtggt gtcccgatac
     1621 caccagcccc aattagcccc agtatagaag ctgaacgtaa gtttagttca aagcgataaa
     1681 gtatgagtga tagaaaggca ggaataattt gtggtatgac tgcaaatacg agtgttttaa
     1741 tcttattcgc accactggcc tttaatgatt ctacagcact gaaatctaga ccttcaatat
     1801 cttcagctaa aagtttccca agcataccta cggaatggat acctaaagct aatacacctg
     1861 aaaatgaacc tgggccaaca gctttgataa atataagtgc cattacaatt tctgggaaga
     1921 cacgtataac acttaaaata aatttgctaa cacctgaaac tgggcgtagc tttaccatat
     1981 tatttgcacc tagaaatgct aatggaatac agataattgc ggcgatgaaa gtacctacaa
     2041 cggctatcgc aaaggtttca agtaaaccac gtaataagtc ttcgccatct ggtatataga
     2101 tatagctgat atcaggatgg aataatccgc tgaatatgga ttttaagatt tctaatgatt
     2161 tacttttaag ttctaaactt ggtacacctg caaatgccca gatgataata gctaagacta
     2221 caattgcaat aagccatctt ttaatcaatt ttcgtttgtg tgcttttgtg tgaacattat
     2281 attttgctat ttcctgtgtc atgcgagatg tgccctcact ttcgtactga tgtaatcaat
     2341 gacgacgacg ataactaaag taaataaaat aatcgttgct gtttttggat attgaaataa
     2401 accaagtgtt tgatcataaa acaatccaat accgccagcg ccgactaatc caagcacagc
     2461 tgaagcacgt atatttactt caaatgcata taatacgtat gacataaatg acgatatggc
     2521 ttgtggtaca acaccgaaaa caatccattt tattttatta gcgccaacag ccgtcattgc
     2581 ttccattgga cctggatcta tcgtttccaa tgattcatat aataattttc caataataca
     2641 gatagttaaa ataaacaatg ctaatatccc tggaatttga ccgattccaa atacagccac
     2701 aaagattgct gctaatagca aatccggtat agtacgaact atatttaaaa taaaacgcga
     2761 gggtattgaa atccattttt gatgaacgat attgctagca cataataacg caattggtat
     2821 tgaaacgatg ctacctaata ctgtacttac gatagccatt cgaatagtat ctaacattgg
     2881 cgttgtaatt tgttgtaaat actcgaaatc aggtggaatc atttgtttga atagatcacc
     2941 tatttgaggt attcctatca ttaaatctcc aaaattaaac cccgtataaa tgaagctcca
     3001 aatgataagc acaatgatta acatgaaggt aaaactcgtt tttaaagaaa cctttttctt
     3061 taaaagggag tcatactttg taggtgtttc taaaggcatg ttagttcact cctagctttt
     3121 catcttcttt aattgtacgt ccatatattt cactaaatac gtcatctgtt gcttcagatg
     3181 caggaccatc atagacaact tcaccatcac gtaaaccaat gatgcgtgtg ccatattctt
     3241 ttgccaagtc aacaaaatgt aaattaatta aaattgtgat gcctaattct tggttgattt
     3301 ttcttaaatc atccataacc tgtttcgttg ttaatgggtc taatgaagca actggttcat
     3361 ctgcaagaat aatttcggat tcttggcata gcgcacgtgc aatagatata cgttgttgtt
     3421 ggccacctga taattcatca gagcgttgat tatatttatc taagatgttg acccgttcta
     3481 gtgcatccat tgctttaatt ttgtcttctt ttggaaataa acctaatacc attttccaag
     3541 tagggtgata accaacacgt ccacttagta catttcgtaa tacacttgac cgtttaacta
     3601 aattaaaatg ttggaaaatc atgcctatat ttcggcgcat ttctaataat gctttaccat
     3661 gggctttagt gattgattta ccttgaatga aaatttcacc tgacgtgata tcatgcaaac
     3721 gatttacaga tcttaataac gtggatttcc cagcaccaga tagtccgaca atgactgtaa
     3781 attcaccttt ttcaatattt aagttaatat ttttcaagcc tacatgaccg ttaggataga
     3841 ctttactgac gtttttaaat tcgatttgac tcatgtttga cacctttctt taataaagaa
     3901 aagccaaggt aaaccgaaat atcatagaca ttgggtttct gtgtacaaat agtttcaaat
     3961 gtacagcgac acctttctat atctgtttca gtttagcgcc tagcttttca atattaatct
     4021 agaatatatc tattgaacga aagcttttaa taccaaattc gataatgatt catttgttaa
     4081 ataatgatta tttcatatct ttaactaatt tttcgtactc tcttacaatg tcgaaatttg
     4141 aatctttcgt ttctgtgtat ccttcatgtg aataaacttc gctaataatt ttgtgacctt
     4201 cttttgattt agcaatgtct acaaaagctt ttttcaattt ctcttggaaa tctttatcca
     4261 tatctggtct tacagaaatt gtgtcattcg gaatagcttg tgttaatttt aaaattcgtg
     4321 tgtctttaaa tacatttggt tggtcttttt tcacagtatt acgtgcatcg ttaaatacag
     4381 ccgcagcatc tacatctcca tttaataatg agataactgc ttggtcatga cctttaacat
     4441 tcacaatttt catatcttta gttgcattaa tacctgcttc gttttttaac atcgcaagtg
     4501 ggaatgtata tccagcagtt gatgttacat cttgtaaggc aattttctta ccttttaaat
     4561 cttttaagct tttaattttt gagtcttttt taacaagaat ttctgattta taactatcta
     4621 caagttcttt acttgctgaa ccatcttctt ttacaccaaa acgttgtgct tgtaataata
     4681 aatcagctgc tttttgatca tgtgctaatg tgtatgccgt tggtggtaag aaaccaacat
     4741 caactttttt agacttcata gcttcaacaa ttgtattgta gttagttgat acagacactt
     4801 taactggaat tcctaattct ttagatagta atttttctaa tggttttgct ttagcctcta
     4861 atgttccagc attttgcgaa ggtacaaatt gtactgttaa ttctttaggt ttgtatcctc
     4921 ctgatttaga atccgaatca ttactagcgt tcttttgatt atctaaagaa cttgaatttc
     4981 cacatgctgc tgcaaaaaca atgactgcta acattaatac aaataaacac ttaaattttt
     5041 tcatttgata actgtcccct tcacattctt aatcgtgtaa tatgtatgca atgaaattac
     5101 acttatagcg taccacattt aaatgcgaaa aagtatttta taaaaatgaa ttttaataac
     5161 tttctttaag agtttgataa gattttgtaa atttttagtt aaattgccca tttcaaacat
     5221 atatttatcc tataattcaa gaacaaattt attttaaaag gagcctcaca aaatgaaaaa
     5281 aatatataag tcattaactg tctctgcaat tgttgcaacg gtatcattaa gtgttttacc
     5341 gcaatcttta gctataacgc atgaatcgca acctacaaag caacagcgaa cggtattatt
     5401 cgatcgttct catggtcaaa cagctggtgc tgcagattgg gttagtgatg gtgcattttc
     5461 agattatgcg gattcaatac aaaaacaagg ttatgacgtt aaagctattg atggtcattc
     5521 gaacataaca gaagcaagtt tgaaaagttc caaaatattt gtaattcctg aggctaacat
     5581 tcctttcaaa gaatcagaac aggcagcaat tgttaactat gtgaaacaag ggggaaatgt
     5641 tgtctttatt tcagatcatt acaatgctga ccgaaattta aatcgtattg attcatcgga
     5701 ggcaatgaat ggttatcgac gtggagcgta tgaagatatg tcgaaaggta tgaatgcaga
     5761 agaaaaaagt tctactgcaa tgcaaggtgt gaaaagttca gattggttat ctacaaactt
     5821 tggcgtacgt tttcgatata atgcactagg tgatttaaat acgagcaata ttgtttcttc
     5881 aaaagaaagt ttcggtatta ctgaaggtgt gaaatctgta tctatgcatg ccggatcgac
     5941 attagcaatt actaatccag agaaagcaaa aggtattgtg tatacaccag aacaattgcc
     6001 agcgaaaagt aaatggtcac atgctgtaga tcaaggtatt tataatgggg gcggtaaagc
     6061 agcatttatc ggtgattcat cacttgtgga agatagttcg cccaaatatg taagagaaga
     6121 taacggagaa aagaagaaaa catatgatgg ttttaaagaa caagacaacg gtaagctatt
     6181 aaataatata acggcttgga tgtctaaaga taatgatggg aaatcactta aggcgagtgg
     6241 cctaacatta gatacaaaga ctaagttgct tgattttgaa cgaccagagc gttcaactga
     6301 gcctgaaaaa gagccatggt cacaaccgcc gagtggttat aaatggtatg acccaacaac
     6361 atttaaagca ggtagttatg gcagtgaaaa aggcgcagat cctcagccaa acacaccaga
     6421 tgatcatacg ccaccaaatc agaacgaaaa agtatcattt gatatcccgc aaaatgtttc
     6481 tgtaaatgag ccatttgaag tgacaataca tttaaaagga tttgaagcaa atcaaacact
     6541 tgaaaatctt agagttggta tttacaaaga aggcggacgt caaatcggac aattttcaag
     6601 taaagataac gattataacc cgccaggtta cagtactttg ccaacagtta aagcagatga
     6661 aaacggaaat gccacaatta aggtcaatgc taaagtactt gaaagtatgg aaggttcaaa
     6721 gattcgttta aaactcggtg acaaaacctt gattacaaca gacttcaaat aaatatataa
     6781 tagaaataag aaagatgttt gtgattgaag gagtgagtga ggatgtcaaa catagcattt
     6841 tatgtcgtga gtgacgtaca tggttatatt ttcccaacag attttacgag tagaaatcaa
     6901 tatcaaccta tgggattgtt actagcgaat catgttatag aacaagacag aaagcagtat
     6961 gaccaaagtt ttaaaataga taatggtgat tttttgcaag ggtcagcatt ttgtaattac
     7021 ttaatcgcgc atagcggcag tagccagcct ttagttgatt tttataatcg aatggcattc
     7081 gactttggta cgcttggtaa tcatgaattt aattatggat tacaatactt aaaagacact
     7141 ttacgcagac tcaattatcc agttttgtgc gctaatattt atgaaaatga tagtacattg
     7201 actgataacg gtgtgcagta ttttcaggtt ggagatcaaa cggttggtgt gataggttta
     7261 acgacacaat ttattcccca ttgggaacaa ccagagcata ttcagtcact tacgtttcat
     7321 agtgcttttg acacacttca acaacactta cctgaaatga agcgacatgc agatatcatt
     7381 gtggtttgtt accatggtgg atttgaaaag gatttagaaa gtggtacgcc gaccgaagtg
     7441 ttaacgggtg aaaatgaagg atatgccatg ttagaagcgt tttctaaaga aatagatatc
     7501 tttattactg gtcaccagca tcgacaaatt gctgaaaggt ttaagcaaac ggctgtgatt
     7561 caacctggta cgagaggtac aactgtaggc aaagttgtct tgagtactga tgaatatgaa
     7621 aatgtatccg ttgaatcatg tgaattactt cctgttatag atgatcccac atttactatt
     7681 gatgaagatg accaacatat acgaaagcag ttagaggact ggttagatta cgaaattact
     7741 acattgccat atgatatgac gattaatcat gcatttgagg cacgtgtagc tccgcatcct
     7801 tttacaaatt ttatgaatta cgctttatta gaaaaaagtg gagcagatgt tgcctgtaca
     7861 gctttgtttg attctgctag tggtttcaag caagtcgtga cgatgcgaga tgttattaac
     7921 aattacccat ttccaaatac atttaaagtt ttagctgtaa gtggtgccaa acttaaagaa
     7981 gccattgaac gatcagcaga atattttgac gtgaaaaatg atgaagttag tgtgagcgca
     8041 gacttccttg aacccaaacc acaacacttt aattatgata tatatggtgg cttaagttat
     8101 accattcatg ttggaagacc aaagggacaa cgtgtgagtg atatgatgat tcaaggtcac
     8161 gcagttgatt taaagcagac atatacaatt tgtgtaaata attatcgtgc agttggcggt
     8221 ggtcagtatg atatgtatat cgacgcgcca gttgtaaaag atattcaagt tgaaggcgca
     8281 caattactta ttgatttttt atcaaataat aatttgatgc gcatcccgca agttgttgat
     8341 tttaaagttg aaaagtgacg gatatatgta aaacttactt gattttaatc gggtaagttt
     8401 ttgtttttgt ttaaactaat cactattaaa accaatatac tttaaggaaa atgttgattt
     8461 tacaaaaata aggcttatat tttatataac ttaatattat gaagtgatta atttggaaac
     8521 aattaaacaa gaagtaagag aatctattgt taaaggaatt atcgcgggat ataaggacta
     8581 tgttaatgag agaaatgaag tcaagaaacg tatggtgatt agtgatgcct atgtgtacac
     8641 caaatctaat catattgaaa atcaagttgc taagcatttg gaaaattttg ttaagtatat
     8701 taaagaaaat gctggaccat catggaagta tttaaagttt atatttaata aagatgaaga
     8761 gaaaaataat atcatgttta tattgaaaaa tgaagattac tttgatgaaa aaaagtaagc
     8821 gtagggaaga gtttagttgc tgataagaat caaaaaagta aaaactacct agaggtactt
     8881 atggctaaga atacggatat taattttgat actgttgaag aagattttga aattaggcat
     8941 caaatgactg cagatagtat attatttaat ataaaagaag gcagtaatag agatattaat
     9001 tcttattttt taattattac atatcgtatt gataaagaat caaagcaatt agctgcaatc
     9061 aaacaatggt tgccaaatcc agaaacaaat tctgcaatta tggttgatga tttgacacca
     9121 ttgttagata aagtaatagt agaaagagaa gattatcata ttgacgaaga agagttagaa
     9181 gtacttaaaa acgatggaga gttagaactc atagatgtgg aacatgcatt tggtatcagt
     9241 atcgatgaag gtaacgatat gattgaaagt gaaaggtgaa gttaatgttt attggcaaga
     9301 atttagaata cgtgagaaag cttaatgctc taagtagaaa agaattatca gaaaaaataa
     9361 atgtgagtga acaagcaatt tggcaatatg aaacaaaaaa tatgatgcca gaaattagta
     9421 aaatttatga tatgacatct atttttaatg ttaaatctag ctactttatt agcgagcagc
     9481 ccgaagagct cctaataaat tctgtcgata aacatagtat agcttttaga gcaaaaaatt
     9541 ataaagtatc tacgaaact
//
LOCUS       DAHHRR010000097         9466 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.097, whole genome shotgun sequence.
ACCESSION   DAHHRR010000097 DAHHRR010000000
VERSION     DAHHRR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.097"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            158..601
                     /gene="mgrA"
                     /locus_tag="NBI42_002027"
     CDS             158..601
                     /gene="mgrA"
                     /locus_tag="NBI42_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HCD1778426.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            complement(728..2401)
                     /locus_tag="NBI42_002028"
     CDS             complement(728..2401)
                     /locus_tag="NBI42_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HCD1778427.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINEGQLNIIYMTSIVL
                     MILTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGNNRLTDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSIRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAIARMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(2398..4029)
                     /locus_tag="NBI42_002029"
     CDS             complement(2398..4029)
                     /locus_tag="NBI42_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCD1778428.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGILVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNNNNEFLLKHEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYHPTIGTISINQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNIVEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            4248..5123
                     /locus_tag="NBI42_002030"
     CDS             4248..5123
                     /locus_tag="NBI42_002030"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCD1778429.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGENDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSVKVKNPQTVD
                     QINYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            5295..5978
                     /locus_tag="NBI42_002031"
     CDS             5295..5978
                     /locus_tag="NBI42_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778430.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     AEKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            5981..6439
                     /locus_tag="NBI42_002032"
     CDS             5981..6439
                     /locus_tag="NBI42_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCD1778431.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVITQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            6441..7007
                     /locus_tag="NBI42_002033"
     CDS             6441..7007
                     /locus_tag="NBI42_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCD1778432.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(7103..7645)
                     /locus_tag="NBI42_002034"
     CDS             complement(7103..7645)
                     /locus_tag="NBI42_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1778433.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(7725..8024)
                     /locus_tag="NBI42_002035"
     CDS             complement(7725..8024)
                     /locus_tag="NBI42_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778434.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGRTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            complement(8162..8563)
                     /locus_tag="NBI42_002036"
     CDS             complement(8162..8563)
                     /locus_tag="NBI42_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778435.1"
                     /translation="MKKLIISIMSVMILLAGCGKSEEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKDKEASQGKQ
                     SKDSTKSSDKDNHKKPTSTDKDQKANDKHQS"
     gene            complement(8631..9074)
                     /locus_tag="NBI42_002037"
     CDS             complement(8631..9074)
                     /locus_tag="NBI42_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1778436.1"
                     /translation="MRALNKDEHNYIKQIANIHETLLSQAESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISSTIHKSNLPMISLNKDLGYQVSHVKMYKDID"
     gene            9201..>9466
                     /locus_tag="NBI42_002038"
     CDS             9201..>9466
                     /locus_tag="NBI42_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778437.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNET"
BASE COUNT         3248 a         1605 c         1393 g         3220 t
ORIGIN      
        1 tgatttaatc aaaaatatct tctaaacttt aaacatactt taattttagc atggcattga
       61 atcaaagaaa gttgtgaatt aataaacaat caacttttta atgattacca atccgaaaag
      121 agaaaaaaac ggatagtatg tctggaggag aactttaatg tctgatcaac ataatttaaa
      181 agaacagcta tgctttagtt tgtacaatgc tcaaagacaa gttaatcgct actactctaa
      241 caaagttttt aagaagtaca atctaacata cccacaattt cttgtcttaa ctattttatg
      301 ggatgaatct cctgtaaacg tcaagaaagt cgtaactgaa ttagcactcg atactggtac
      361 agtatcacca ttattaaaac gaatggaaca agtagactta attaagcgtg aacgttccga
      421 agtcgatcaa cgtgaagtat ttattcactt gactgacaaa agtgaaacta ttagaccaga
      481 attaagtaat gcatctgaca aagtcgcttc agcttcttct ttatctcaag atgaagttaa
      541 agaacttaat cgcttattag gtaaagtcat tcatgcattt gatgaaacaa aggaaaaata
      601 attaactttt gtcatgacaa ttaaagtaat gtttagaatt tattaagaat agaaaaacaa
      661 ttagcacgcg taagcttgtt agttaaaaaa ctgcttgaaa ggtttcttag cctatcaagc
      721 agttttttta tgcattatat tgaatcatat tccataaagc accttgatta gcgagtaatt
      781 gttgatagtt tcccttttca actattttac catttatcat cacaatgatg gtctcaaaac
      841 gtgacaataa agttaaatcg tgtgtagcaa caattaatgt ttctgcatgt gcttcaatta
      901 aatccataac ttttaaacta ttttgtttat ctaatgccgt tgttggttca tctaaaatcc
      961 atgttgatgc agtatctttt aataacatcc tcgcaatcgc taaacgctga atttctccgc
     1021 cagataatgt atgaccatct aagtcaattt gacgttctag tgccaaatgt tctaaatcta
     1081 attgcttaaa tattgcttgc accgcttcat ctttttcatc ggtaaataaa ttttgacgta
     1141 ttgtaccatc aaataattgt tgagattgta gcaagacatt caacgattca aacttatctt
     1201 tgtcatctat ttcaaacata tccatatttt cgaaacgaat agagccacta tctaattgat
     1261 ataaccctgc cataatttgt agtaatgtac ttttccctga accagaagga cccacaatcg
     1321 caatcttttc gcctctatta acattaaaat taatatccga taacacatac gtttgctggt
     1381 tccaatactt aaaactagcg tccttaactt cataaatgtt cgttgcgtca tacttatcag
     1441 taaggcggtt atttccatta gtagaaggta cagatatcac ttcattaata tcgtgcaatg
     1501 cttggtcagt atccgcttta taatacgcga catttgtcat tggtacagct tgttcaaata
     1561 aagttaagat cattaaaacg atactcgtca tataaataat atttagttgg ccttcattaa
     1621 tttgaattaa tcctagaact aaactaccaa aaatcgaaag catagcaata atatttaata
     1681 taaaatcata aatcgttaaa aagcgttgct ctttaagttg taatttatca aaatgattta
     1741 atttagccat caaattatct cgataatgat taatttgatt aaatcgacgt agttcatcca
     1801 taccagcttt ataatcataa aaatgattta aaaatcgagc ctgttcatta gctgcatgtt
     1861 tctttaaagt acgtgctttt tttgagctta accaaggaac gatgagtaaa gtaaacaaca
     1921 tgctaaccat aatcaatagc gcatggccga ttgaaatgaa cgccaatact attactgtaa
     1981 ctagcgctgt caaaccgatg acgactggtg gataataaac acgtaaataa atattttgta
     2041 acgcctcaac acgactaatc atacgtgaaa ttaaatcact agaactcagt ttacggtaaa
     2101 cattaggaat gacatttact aatttaccga aaaactgtac ccgaatatca cgtagcattg
     2161 taaatgtagc tttatgagaa ataaggcgct ctacgtatcg agtaatagct cttaaaaatc
     2221 caaacaattt tactgtaacg actaaaatca tcagagcgta tagtggcgca ccaagtgcac
     2281 tttgtgtcac catataacca cttaagaaaa acatggcgag cgcaactaaa cttccacaaa
     2341 caccaacaac tatagctaac aataaatcct tatctacttg aaattttagt cgtgttttca
     2401 taagtcatca ccattttctg ttatatttac tgaaatattg cggtcatcag caatcagtct
     2461 tccattttct atatacaaac gtcgttgtaa atggcgaatt gtattatctc tatgagcaat
     2521 gacaatcatc gttgtgtctt taaaatgttg aaacagaacg ttctgaatca tgtgttctgt
     2581 ttgaatatct aaaccagttg caggctcatc aaatataacg agatctggct tcataactaa
     2641 aagacggcaa agttctatgc gtctcatctg tccaccagat aacatttcgc ctccttcacc
     2701 tattattgta ttaatgcctt ttgtgaaaga ttgaactttg tctaataaac ctacttcgtc
     2761 tagcacttct tcaacaatat tattttctat atctttaaac atcgtaatat tctcttttat
     2821 agaagcactg aaaatatatg gctgttgact aagtattcct atatttaaat cacgctggtt
     2881 tatacttata gtacctattg ttggatgata aacacctgca ataagatgtg tcaaagtgga
     2941 tttacctgcc ccgcttggac ctacaagtgc aatttgatca cctttaaata tttccaaatt
     3001 taaatcattt aataccaatc tatcagaatc atcatatcga aatgatatgt ctgttaactg
     3061 aataaatggt ttttgatttt cctcatgctt taatagaaat tcattattat tgttcggttg
     3121 ttctaaaaac tcaaacacaa cgtcactggc accttcactt tgttttccag tatggaacgc
     3181 ttgtcctaag tccttaattg cattataaaa ttcaggcgct aaaataatcg caattgccgc
     3241 agttttaaaa tcaatattat gaaatactac taagcttagc gttgcttcca atgcaaccaa
     3301 tccaatgcct aacatactta taaattcgag cattaatccc gataaaaaag cactgcgtaa
     3361 aatgcgcatt gttaaagttc taaactgagt actatcgtcg taaatatgct tctctgtttg
     3421 ctctgtacga ttaaatagct ttaacgtcac taaaccttta gcaatattta aaaaccgttg
     3481 actaaattga ttcaaataag tcatttgatc ttttgactca tctcgcgttt tcaaaccgaa
     3541 aataatataa aacaaaggaa taaatggtgc agttattaac ataattaatg cggtattgaa
     3601 atggatgaaa aacattgcaa tgattatgat gagcggaacc atcattgatt tgaacacttg
     3661 aggcaaataa ctcttataaa aaggtgctag accatcaatg ttttctgtga gtatagtcat
     3721 ttgttcaccg attggatgac cattattttt ataaataacc cgctgtctaa gcatatgctt
     3781 aactttaaat gctaatgtat cacctaacca ttgatttaga aattgcacag ttgctcttaa
     3841 aagtaaaaca cctaataaaa taaataatac aatccataaa ccttgaaatt gatgtcttat
     3901 aattttagct aaaaaatctg ctattaaaat attttgcgtt ataacgagta tgccgagacc
     3961 agtactgacc aagaacatga gtaccggaaa aattttatat tgaaatagta ttgttgttaa
     4021 ttttttcaca attatatcac ctaaacctat tataaaaggt tttccttaaa atcaaattaa
     4081 atagctcaat aaaatattgt agttttacat gtaaaagaaa atgaatatat acatctttag
     4141 tctagtatta taactataac tagcatgtct ttaaatgaat atgctatcat gatatggtaa
     4201 ttttgatatt taaataaatt aaattgagta atgaaagtgg gtacattatg tttatcattg
     4261 aattaataaa aggtattatc ttaggggtcg tcgaaggatt aacagaattt gcacctgttt
     4321 cctctactgg acatatgatt ctagttgatg atatgtggtt aaaatcatct gaatttttag
     4381 gttctcaatc agcatttaca tttaaaattg tcatccaatt aggatccgtc tttgcagcag
     4441 catgggtgtt ccgcgaacgc ttcttagaga ttttacatat tggtaaacac aaacatgttg
     4501 aaggagaaaa cgatcaacaa agacgttcaa agccaagacg tttaaattta ttacatgtat
     4561 tagtgggtat ggttccagca ggtattttag gcttactatt tgatgatttc atcgaagaac
     4621 atttatttag tgtgccaact gttatgattg gcttattcgt aggtgctatc tatatgatta
     4681 tcgctgataa gtattcagtt aaagttaaaa acccacaaac agtggatcaa atcaattatt
     4741 tccaagcgtt tgtaattggt atctctcaag cagtagctat gtggcctggt ttcagccgtt
     4801 caggctcaac aatttcaact ggtgtattaa tgaaattaaa tcataaagca gcatcggact
     4861 tcacatttat tatggctgtt ccaattatgt tagcagctag tggtttatct ttattaaaac
     4921 attatcaaga tattcagatt gctgatatac ctttttatat tttaggcttt ttagctgcat
     4981 ttactgttgg actaattgca atcaaaacat tcttgcatct aattaacaaa atcaaattaa
     5041 ttccatttgc catttacaga attgtcttag taatctttat tgctatactt tacttcggat
     5101 ttggcattgg taaaggtatt taatttcctt ttaaaatata gactgtcaat taaggaaccg
     5161 cttgattggc agtttttatt gtcttcttta aaaaaacatc tgaaatactt ataagtgatt
     5221 cactactctc atcgtttata ttgatgtatc atagggtatg taaaattaat aaagtaatta
     5281 tggaggcaag gtctatggat aagaaaaaag tcatcaaatt tatgattaat gtattaccaa
     5341 ttgtattggt accgttaatt gttgaacgta aacgtatcaa acaacatccg gacgtacaaa
     5401 aagttacaga tgctacaagt aaagttgctt caaaaacatc tgcagctatc agtaacacag
     5461 cgagtgatgt taaagaatat gtcggcgata aaaaacaaga ttttgaaaat aagcgtgaac
     5521 ttaaaaagtt tgccagagaa catgatcctg cctatattga gaaaaaaggc gaaaaattag
     5581 ctaaacaaaa tcgtaaagac gctgaaaaaa tgaataaaat acttcaaaaa aatatcgaaa
     5641 agcgtcataa agaagagcaa aaagcccgcg aaaagaatga aatacaacgt attaaagata
     5701 tgaaaaaatc acaaaaatac gaagaaaaag caggcttaac acctaataaa ttagatgaga
     5761 aaactgagaa aaaaggcgat aaactagctg aaaaaaatcg caaagaaatc gctaaaatga
     5821 ataaaaagtt acaaaaaaat attgaaaaac gacacaaaga agaacaaaaa cgccaacaag
     5881 aagctgataa agcacgcatc aagtcattta aaaaatataa agattatgtt gccaaaagcg
     5941 cctctcaaca aaataaagaa aacaatacag aggcataatt gtgacacata ttattattga
     6001 tggagatgct tgtcctgttg ttgattctat tatagattta acaactgaga caggcatttt
     6061 tgtgacaatt attcggagct tcagccattt ttcgaaccaa ttatatcccc cacatgtatc
     6121 aacattatat gttgatgatg gaccagatgc agttgattac aaaattgttc aattatcaac
     6181 gaaagatgat atcgtaatca ctcaagatta tggactcgca agtttattag tcgacaaagt
     6241 cttaattgtc atgcatcata atgggaagat atacaattcc aaaaatattc aacaactatt
     6301 agataaacga tatatgaatg cacaaattag aaaacaaggt ggtcgtcaca aaggtccccc
     6361 accttttaca aagcaagatc aaaaagtgtt tgaacaatca ctattaaaag tcattcatcg
     6421 aattaaggag ctggattaat atgaagcgaa tcgcagttta ttgtggtgca agcaaaggtc
     6481 atgacccttc atatgtacaa aaagcatacg atttaggtaa atattttgct gaacaaggtt
     6541 atgaattagt atttggtgca gggtcaattg gtattatggg tgccattcaa gatggtgtat
     6601 tagatcatgg cggcaaagct attggcgtca tgcctaaaat gttagatgaa catgaaatca
     6661 caagccaacg tttaaccgaa ttaattttag ttgattctat gcatgaacgt aaaaataaaa
     6721 tggcagaact tgcagatgca tttgtcatgg ctcctggtgg cgcaggttca ctagaagaat
     6781 ttttcgaaat gtatagttgg gctcaaatag gtatccatga aaagcctatt gccatttata
     6841 atattaatgg ctttttcaat ccactacaaa caatgattga ccatatgatt gaggaaggtt
     6901 ttattgatcc taagtatcgt gcacttgcac cgttatgcga cactaaggaa tcattaatag
     6961 aatcaatatt aaatttcaaa cctctaggca ctcgttcata cgattaatca ttctaaggtt
     7021 aaaaaataaa caaacacccc taatattgtt atcatcagct aaacaatgtt aggggtgttt
     7081 gttacgctta tgtataaatt tttcactttc gaagattaat gtaatagcgc acttgccttt
     7141 ttaataaatg atacttactt agattgggta attcgaaatg atcatacact gtcatgccaa
     7201 ttttttccat tacacgttgt gaagctttat ttgcttctgc tgtaaaacta tagacatcgt
     7261 atatttgatg ttcttctgct aacttcaacg ttgcctttgc gccttcagtt gctaatcctt
     7321 ttccccaaaa ttctggcaac aagcgccaac ctatttcata aagcggtaat tctttaaatg
     7381 gataatcgct tgtttctgga atataattca aacctataaa gcctatccat tgatgggact
     7441 ctttatcttc tacagcaaat aatccaatgc cataatcttt aataaccgca tccatagttc
     7501 tcatatctaa ttctgaacga cgataactca ataaacttgg aaaatattta cgtacgtcat
     7561 aattcgcatt cattttttga aaaggtaaca gatcatcttc atgccagtct cttaatatta
     7621 aacgctctgt ttcacaataa atcatcgatt gacactccta tataatttct caataaaaaa
     7681 cacaaaatcg atattttgat caactttgcg ttttagatag cagtttattt tgatttagaa
     7741 atatagctta taagttctac atgaactggc acttctttta aacgtgctct atctgatttc
     7801 agcgcagtaa aagttgcatc tataaaatct tgttgatggt ttaaacgata tgtagcaaca
     7861 aaagtatctg tatcggactc aaagtttggt aaatgtgcaa ttacacgctt aatttcgcca
     7921 gtagaatcag caatcgtgcg ccctgtaata ttttctaatt ctctacctaa ttctggcaaa
     7981 gtaatatctt gaccctctaa aattctaaaa tcttgttcat gcatgccgtt tctctccttt
     8041 attaattacg ttgtctattt ttacgttctg tttcaatcat gaccacattt taattaatac
     8101 atacccttaa aataaggttt aaaacgctgt acctttcatc taaaacaagt aagcaattca
     8161 attatgattg gtgtttgtca ttagcttttt gatctttatc tgttgatgta ggctttttgt
     8221 gattatcttt atccgatgac ttagttgaat cttttgattg cttaccttgt gatgcctctt
     8281 tatctttatc tttatcttta tctttatttt tgtcttcgtc ttttttatca tccttgttat
     8341 cttctttaga aggttttaaa tctttcactt ctttttcaag gtctttttgc ttatctgcta
     8401 atttttcttt ttcttgttga agcttttctt ttttatcttt taattcttta ttttcttttt
     8461 gtaaattatc gatatctttt tcaagagttg ctttttcttc gcttttaccg caacctgcta
     8521 ataaaatcat aactgacatg atgctgatga ttaatttctt catataaaac tccttaacgt
     8581 catttaaatc ttttttaatt ttaacatgaa ttagcaacat aatcctaatt ctaatcaata
     8641 tctttataca ttttcacatg actcacttga taacctaaat ctttattcaa agatatcatt
     8701 ggcaaattac ttttatgaat tgtactgctt attcgctttg cattcatagt ttttgcccat
     8761 ttttcaagcg caatcttcag ttgcgtagct attcccagtt tgcgaaattg tggttcaaca
     8821 tatagcagtt caatgttaac catacttttt tcattactaa aatgtcccca aataaacgct
     8881 attaattgac cttcattttc atatatataa attttatcat ttgtatgttc taatcttgaa
     8941 catatcatct cgtacctaag agcaatactc agtttagtac atttatagtt ggattctgct
     9001 tgcgacaata atgtctcatg tatattagct atttgcttga tataattatg ttcatcttta
     9061 ttaagtgccc tcattttcct ctccaatatt aatcgttata tttatgcgtt taatatgata
     9121 tgattgacct tattaatact ataaaatcat ttccatttta atttggaatt aaacattttt
     9181 aaaggagcgc ttaagtaaca atgaaaagta ctgcgcaatt aacaaaagaa aataatgtta
     9241 aatcattaag attaagcaat actgatagag aaatcttcga gaattatatg acttatatgc
     9301 gctctgactt tcgcgtcaat cctcatgata cagagttaat tattaaccgt atacttaaac
     9361 aattattaag cgcagaacaa catggtttat tagccttaga ttttttcaat cacgacccaa
     9421 aagcacatgc aataaaagaa ctaaaagcaa tgcctaatga aacatt
//
LOCUS       DAHHRR010000098         9402 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.098, whole genome shotgun sequence.
ACCESSION   DAHHRR010000098 DAHHRR010000000
VERSION     DAHHRR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.098"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..465)
                     /locus_tag="NBI42_002039"
     CDS             complement(<1..465)
                     /locus_tag="NBI42_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCD1778438.1"
                     /translation="MKIKTEFKGNNIPYEYAAGTDVSDSINGNPIKSFPFEVTELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRIKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDKDHQYELAVYALDHSLNLKNGFYLNEFLKEVN"
     gene            596..1465
                     /locus_tag="NBI42_002040"
     CDS             596..1465
                     /locus_tag="NBI42_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1778439.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNENPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYDKAQFLSIND
                     VHTSIKLIEMNQGISFLPIYITTNSDYNISIINTKILQAPISFTYIYSKKESPEILAF
                     IKSFKKYIANEQL"
     gene            complement(1524..4133)
                     /gene="clpB"
                     /locus_tag="NBI42_002041"
     CDS             complement(1524..4133)
                     /gene="clpB"
                     /locus_tag="NBI42_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCD1778440.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIAKLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(4336..6150)
                     /locus_tag="NBI42_002042"
     CDS             complement(4336..6150)
                     /locus_tag="NBI42_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1778441.1"
                     /translation="MNKTKGFTKYKKMRYMPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLLIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            complement(6718..7026)
                     /locus_tag="NBI42_002043"
     CDS             complement(6718..7026)
                     /locus_tag="NBI42_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCD1778442.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            7140..7964
                     /locus_tag="NBI42_002044"
     CDS             7140..7964
                     /locus_tag="NBI42_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1778443.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            8016..9332
                     /locus_tag="NBI42_002045"
     CDS             8016..9332
                     /locus_tag="NBI42_002045"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCD1778444.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEIVAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGV
                     DKRIDVLSMAMMNKLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
BASE COUNT         3154 a         1663 c         1323 g         3262 t
ORIGIN      
        1 atttacttct tttaaaaatt cattcaagta gaacccattc ttcaaattta aagaatgatc
       61 taacgcataa acggctaatt catattgatg atctttatca ggtggtgttg gtcctacata
      121 gtgattttct atttctgaaa aatcttcagc caacaaccca ctagtaaagc tatttttacc
      181 ttgtacatag tcacccttta ttcttgataa atctgctttt atagaaattg aattgccact
      241 aacacttaca ttagctacac tccaatgaat ccaagcaaag ccacatacag gaattgcatc
      301 atagtcaatt aaagaccaag caagatattt agtcccttcc ggtaattcag ttacttcaaa
      361 tggaaatgat ttaattggat tcccgttaat agaatcactc acatctgtac ctgctgcgta
      421 ttcatatggt atattgttcc ctttaaattc agtttttatt ttcatgttgc tctctccttg
      481 taaaattttt tattcattca accttaacct ctgatataat attaatatta tttttatgca
      541 ataaaaatta aatattatct aatgaattat taactttttt gaaagaggta agttcatgaa
      601 tttggattgg tactatacat ttgtcatttt agcgaagacg ttaaactatc gattagctag
      661 tgaagaaatt aatctcacta taccttctat acataagcaa attaaaaatt tagaacagca
      721 tctcaatgtg aaactatttg aaacttataa aaatcaaatt atcttgactg aagatggtca
      781 tacatttctt ccaattgcac aaagttttat tgaacaatat gaaagtggca tcaaacatat
      841 ccaacttaaa aagacgatgt ttcaatcgaa attaaatgtt gtcgtgtctt catatatcgc
      901 gacattcatt atgcccaaat ttttaaaatc attctttaac gagaatcctt ttatcgatgt
      961 ttctcttcat gtcaaaaatg aaaatattga aaaagatatt aataatcata cgtatgacat
     1021 cggaattagc agaaatcaac ctaaactaag agaagttcat tctgaaaaag tatgcgaagg
     1081 taaaattgta ttgattgctc ccaataaaga gaataatcat cttctaactg aggcatcttt
     1141 atttgaaaaa tacaaaataa taagtgataa ccatccagaa tattggtcat cactaaaaaa
     1201 taatatttta aatatttatg ataaagcaca atttctatct atcaatgatg tgcatacttc
     1261 aattaaatta atcgaaatga atcaaggtat atcattcctg cctatttata taacaacaaa
     1321 tagtgattac aatatttcga ttattaacac gaaaatttta caagcaccta tttcatttac
     1381 atatatatac agcaaaaaag aaagtccaga aatattggcg tttattaaat catttaaaaa
     1441 gtatattgcc aatgaacaat tataataaat ttcaaatcta aaaaaccaag aatgcgatta
     1501 atcttcacat tcttggttca attttattca tgaatttttt caacattaaa cgttaagtta
     1561 ttatctgaat ttaaattaac tttaatcgtt gtaccttctg ggaatccctc tttaatcatc
     1621 atacgtgcta atggtgtttc aatttggcgt tgtacaaaac gttttaatgg tcttgcaccg
     1681 tattgaggtt cataagcctc ttgacctagc caagctttag catcatcaga aacttcaatt
     1741 gagattcgtt gttctagtaa tcttatattt aattgcgtta agattttatc tacaatcata
     1801 ctcatgtcat caatagatag aggtttaaat aatacgatgt catccatacg attcaaaatt
     1861 tctggtttga aatatgcatt taaacttgtc ataacagctt tttctgttga ttctgtaatt
     1921 tcaccagtct cttttacgtt ttctaataaa acttgagatc caatattact tgtcataata
     1981 ataatagtat ttttaaaatc aacgctacgt cctttagaat cagttaaacg gccttcatct
     2041 aaaatttgca ataatacatt aaagacgtca gtatgcgctt tttcaacctc atctaataaa
     2101 ataactgagt atggattacg acgaaccgct tcagttaatt gacccccttc atcatgtcca
     2161 atatatccag gaggtgcacc tatcaatctt gatactgcat gtttttccat atattcactc
     2221 atatcaatac gaatcatatg tttttcagaa tcaaataatg atgcagctaa tgatttagct
     2281 aattcagttt tacctacccc agttggacct aggaacaaga aactaccaat aggtctattt
     2341 gggtctttaa tacctgctct tgctctaact actgcatctg aaaccaggtc aaccgcttta
     2401 tcttggccta caacacgttt atgcaagata tcacttaagt gaagtaattt ttcacgttct
     2461 gtttcaacta attttgaaac tggtatgcct gtccattggc tgacaatatc gccaatttct
     2521 tcgtctgtta caacttcacg aatcattcga tctgtatctt caccttgctc atcttggaaa
     2581 ttatcctcta attctctaag ttctttttcc aattgaggaa ttgttccata ttgtagttca
     2641 gcagcttttt ctaaattgtt atttgtttgt gcatcttcca acgcttgtct actttcatct
     2701 agttgcgcac gtttttcttg taactttgct attttttctt tttctgattc tacacgagat
     2761 tgaagtgctg cttgtttctc tttttcattg gcaagctctt cttgtagttc ttgtaatctc
     2821 tgtttgctcg cattgtcaga ttcattttta agtgcgcttt cttcaatttc taattgcatg
     2881 acacgtctat taacttgatc caattcagtt ggatttgatc ccatttccgt acgaattgtt
     2941 gcacatgctt ggtcaactaa atcaatcgct ttatctggta aaaaacgatc agtgatgtaa
     3001 cgatcagaca attcagcggc agcaactaag gctctatctt gaatacgcac accatgatac
     3061 acttcatatc gttcttttaa accacgtaaa attgaaattg tatcttcaac atcaggctca
     3121 ctaactgcta ctttttggaa acgacgctct aatgccgaat ctttttcaat atattctcga
     3181 tattcattta aagttgttgc accaatacaa tgtaactctc ctcgtgctaa cattggtttt
     3241 agcatgttgc ctgcatccat ggcaccatct gttttaccag cacctacaag catatggatt
     3301 tcatcaataa ataatataat tctaccatca gactctttaa cttcttttag gactgctttt
     3361 aatctctctt caaattcacc acgatattta gcgcccgcta ctagtgcgct taaatctaac
     3421 tcaaaaacag ttttatctaa taatgattct ggcacatctt tcttaactat acgctgcgct
     3481 aatccttcaa caattgcagt tttaccaaca cctggttcac caatgagcac agggttgttt
     3541 ttagttttac gacttaaaat acgaatcgta tttcgaattt cttcatctct tcctataaca
     3601 ggatccattt taccttgtct aacttcttct actaagtcgc ggccatattt agctaatgct
     3661 tcgtaattaa cttcaggatt ttgtgatgtc acgtgatttc cccctcttac ttttttaata
     3721 atttctttga taacttctac tttattattt atataatgtt ttgttgtttg atcaatgtcc
     3781 attgccgaac gtaaaatatg ctccattgaa atatattcat cttcatattc tttcatgtat
     3841 gattcagcct tagttatcaa ttggtttgct tgttggctaa tatattgacc atattgtata
     3901 ttgtcacctt ctacagatgc atacgtattt agtttgtctt cataagcttt gtttaattga
     3961 tctacctcaa tatttgctcg ttctaaaata cttttatata agctttcact ttcatttaag
     4021 gcagcgctta aaattgcctc aatttctata ttttgtaatt tatgttgctg acttagttca
     4081 actgcttgtt gtaaagcact ttgaacagca tatgtcattt tatttatatc cattaatttc
     4141 acctctaatt tgtagagttg gtcaagttgc acttttgact ttgactttct ttgaccttaa
     4201 ttatattata gtcccaatat aatcatttat caactctttt acacttgaat ttcctaatat
     4261 ttgtaaataa ttttttgaat taaaaaaatc tagctaatgt tacgttacac attaactaga
     4321 cttattaaac atatgttatt tctttttaag tgcttttaat atttcatctt ttaaagctag
     4381 aactcctttg tactctaaat gtacaccgtc tggtgcaaaa tattcactat gtccttgtga
     4441 tcgcttatac caatcaatta atgtgacatt ggactttcgt ttcgccgcgt cagctaataa
     4501 tcgatttaca tttgcctcat aaattcttgg aacacgtgta ttaactaaat aaatcttggc
     4561 ttttccaaat tgattaagta aatcgtcgag ctgtttgaca gtaaagtcgc catttgtacc
     4621 taattctaat acgacttgat cagatgattt tttatattgt gaataattcg ctttaactaa
     4681 aggtaaggtt tgatacaatt gacgccctac ttttccatca attctagact taggaactga
     4741 tgacttaaat gactcaccga tatcaaccat tactgaatca ccgattaaaa gaggtttaat
     4801 attattatat acgtcggaag attccttttt cttctcactt actaagccat caatgtgaat
     4861 gttatcaatt ggtgctatcc gcattaaata attatcttca atagtggtat caaagctatt
     4921 cgctttttct cctataatgt ctttaccata tttattgaat gcacctacta aaatcaacat
     4981 aaatggaatt aacaaggtta ctacaattgc cattcttata aattgtggta tataagaagg
     5041 tcgccaattt aaagctttaa taccttcttt tctaaatgga gtttctataa agcgatatga
     5101 tagctctgca aatataattg ttaaacttat atctataaag tacacatata caggtatctg
     5161 tccgtctaca tagtaactat gtacgaaact aattactgca aaatgccata aatatagact
     5221 gtaagacctt ttcccgataa atactaacac tggatttgaa aatatcttcg ctatccatgt
     5281 agatggatga acgacactgg caataataag taacgttaat atggatatta aatagaaacc
     5341 accatcatat atccaattcg tctcatcatt aataatgaaa aataataata taagtactat
     5401 aaatgataaa ctacctatgc tatcaataac atattttaca actttaggtg gatcattttt
     5461 caatttaaac ggtggccata aaaaagctaa aataacaccc agtaacaatg tctgtaatct
     5521 tgtatctgtt ccaaaataca ctcgtgaatg atccccatta atactgtaga taaacatcat
     5581 taaccctaaa gaaattatag atactcccca aaaaataaat cctattttgt atcgtttttt
     5641 aattgttaac aataatgtaa ccaaaataac tgggaaaaat atgtaaaact gttcttcaat
     5701 tgctaaagac cataaatgct ttaatggcat aaatgaaaat tgctcaaaat aattaacatc
     5761 ttttgctata taccaccagt ttgatacata aaatatcgca gcaataatat catgtttaac
     5821 cctaatgata ttatctgatt ttaataataa ggttgctgtc cctacaacca ttaataaaac
     5881 tatgactgct ggtaataaac gttttaaacg acgtatccaa aagcttttca atttaatgat
     5941 acctgtgtca tcatactctt tgagtaataa gcttgtaatt aaataaccag agatcacaaa
     6001 aaatgtatcc acacctaaaa agccacctgt caaccattgc ttatttaagt ggtaaataat
     6061 aattcctaga acagcgattg ccctcaaacc atcgagccct ggcatatatc tcattttctt
     6121 atattttgta aaaccctttg ttttgttcat ttttgcattc ttccctttta aaactgttct
     6181 tcttagatgc ttaattaaat ttagttatgc tgtttaaaag aatattgaaa tgcatatgta
     6241 tattattgaa ttacgacatc atcaaaatca tattgactaa aatactgtta aattaaaaaa
     6301 ttaccaatga tgattcttat ttcgaaatcc aatttgtaat gcaactcggc aacttaaaaa
     6361 ctatgaagta ttatgtattg taatataact gtaatataaa ttcaatttat tatagaaatt
     6421 ttcaagaaaa tattcaacta agaaatgaat tgtgtactct tagaattgcg agtcactgtc
     6481 ttagttcata ttttttgaaa caagttaatt ataaattttc aaaataaaat cagaaataag
     6541 gctcccttca atgtctactt tgaagggagc tcattcacaa tgaattatac tctacaatgt
     6601 tatattgact gcggggcccc aacacagaga atttcgaaaa gagattctac aagcaatgca
     6661 agttgggagc ggagccccaa ttagatttcc aatttctaca agcaatgcaa gtttatgtta
     6721 gctcacacca agtgcaatct tagcgtaacg tgacatcata tcttttgtcc aaggtggact
     6781 ccatacgata ttcacttcag tatcctgaat ttcaggaatc tctgctaata ctgttttaac
     6841 ttgatcaata atttgaggcc ccattggaca tcccattgat gttaaagtca tatcaactgt
     6901 gcatacgcct tcatcatcaa cattcacttt gtatactaaa cctaaattaa cgatatcaat
     6961 tcctaattca gggtcaatta ccatttctaa tgcacctaag atactatctt tcaatgcctc
     7021 ttccatccat atcacctctt taatgtcata ttattcataa tatatcaaat atccgacaaa
     7081 acgccaataa aatgctatga tgtatctata tgaactaagc aacttatgag gagagagata
     7141 tgcaaccaca tttaatatgt ctagacttag acggaacatt attaaacgat aacaaagaaa
     7201 tttcatcata tactaaacaa gtattaaatg aattacaaca acgtggacac caaattatga
     7261 ttgcgactgg cagaccttat cgtgcaagtc aaatgtatta tcatgaatta aatttaacga
     7321 caccaattgt taattttaat ggcgcttacg tacatcaccc taaagataaa aacttcaaaa
     7381 cttgccatga aattttagat ttaggcatcg cacaaaatat tattcaagga ttacaacaat
     7441 atcaagtatc gaatattatt gcagaagtga aagattatgt tttcattaac aatcatgatc
     7501 caagattatt tgaaggtttt tcaatgggta atccaagaat tcaaactggt aatttacttg
     7561 tccacttgaa agaatcccct acctcaattt taattgaagc cgaagaaagt aaaatacctg
     7621 aaatcaaaaa tatgcttact catttttatg ccgatcatat tgagcatcga cgctggggcg
     7681 caccattccc tgtcattgaa attgtaaaac ttggtattaa taaagcaaga ggtattgaac
     7741 aagttagaca atttttaaat attgaccgaa ataatattat tgcattcggt gatgaagata
     7801 atgatataga aatgattgag tacgcccgcc atggagttgc tatggaaaat ggtttgcaag
     7861 aacttaaaga tgtagcgaac aatattacat tcaacaataa tgaagatggc attggtcgat
     7921 atttgaatga tttctttaat ttaaatatta gatattactg ttaatttata actaatcatt
     7981 ttataatatt ttaaaacaat aggaggtaag ttacgatgcc caaaatagtc gtagtcggag
     8041 cagtcgctgg cggtgctaca tgtgccagcc aaattcgacg tttagataaa gaaagtgaca
     8101 ttattatttt tgaaaaagat cgtgatatga gctttgctaa ttgtgcattg ccttatgtca
     8161 ttggcgaagt tgttgaagat agaaaatatg ctttagcata tacacctgaa aaattttatg
     8221 atagaaagca aattacagta aaaacttatc atgaagttat tgcaatcaat gatgaaagac
     8281 aaactgtatc tgtattaaat agaaagacca acgaacaatt tgaagaatct tacgataaac
     8341 tcatattaag cccaggtgca agtgcaaata gccttggctt tgaaagtgat attacattta
     8401 cacttagaaa tttagaagac actgatgcta tcgatcaatt catcaaagca aatcaagttg
     8461 ataaagtatt ggttgtaggt gcagggtatg tttcattaga agttcttgaa aatctttatg
     8521 aacgtggttt acaccctact ttaattcata ggtctgataa gataaataaa ttaatggatg
     8581 ccgacatgaa tcaacctata cttgatgaat tagataagcg ggagattcca taccgtttaa
     8641 atgaggaaat tgttgctatc aatggaaatg aaattacatt taaatcagga aaagttgaac
     8701 attacgatat gattattgaa ggtgtcggta ctcaccccaa ttcaaaattt atcgaaagtt
     8761 caaatatcaa acttgatcga aaaggtttca taccggtaaa cgataaattt gaaacaaatg
     8821 ttccaaacat ttatgcaata ggcgatattg caacatcaca ttatcgacat gtcgatctac
     8881 cggccagtgt tcctttagct tggggcgctc accgtgcagc aagtattgtt gccgaacaaa
     8941 ttgctggaaa tgacactatt gaattcaaag gcttcttagg caacaatatt gtgaagttct
     9001 ttgattatac atttgcgagt gtcggcgtta aaccaaacga actaaagcaa tttgactata
     9061 aaatggtgga agtcactcaa ggtgcacacg cgaattatta cccaggaaat tcccctttac
     9121 acttaagagt atattatgac acttcaaacc gtcagatttt aagagcagct gcagtaggaa
     9181 aagaaggcgt agataaacgt attgatgtat tatcgatggc aatgatgaac aaactaactg
     9241 ttgatgagtt gactgagttt gaagtggctt atgcgccccc atatagccac cctaaagatt
     9301 taatcaatat gattggttac aaagctaaat aaatgaatca tgcgattcaa tctaatctta
     9361 attatctagg ttagaattat gtgagtctgg gacataaatc cc
//
LOCUS       DAHHRR010000099         9274 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.099, whole genome shotgun sequence.
ACCESSION   DAHHRR010000099 DAHHRR010000000
VERSION     DAHHRR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.099"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..325
                     /locus_tag="NBI42_002046"
     CDS             <1..325
                     /locus_tag="NBI42_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCD1778445.1"
                     /translation="LMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMI
                     KPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSNSYG
                     NASGL"
     gene            382..1356
                     /gene="holA"
                     /locus_tag="NBI42_002047"
     CDS             382..1356
                     /gene="holA"
                     /locus_tag="NBI42_002047"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCD1778446.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKSPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKNHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLMLELFILSL"
     gene            complement(1401..1652)
                     /locus_tag="NBI42_002048"
     CDS             complement(1401..1652)
                     /locus_tag="NBI42_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCD1778447.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            1998..3821
                     /gene="lepA"
                     /locus_tag="NBI42_002049"
     CDS             1998..3821
                     /gene="lepA"
                     /locus_tag="NBI42_002049"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCD1778448.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCILRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            4440..5564
                     /locus_tag="NBI42_002050"
     CDS             4440..5564
                     /locus_tag="NBI42_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCD1778449.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRNLKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     LSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSRRFKKKFDQSIESVFG
                     QTINNLKEKQLIVEKNDAIALTNRGKVIGNEVFEAFLIND"
     gene            5664..6641
                     /gene="hrcA"
                     /locus_tag="NBI42_002051"
     CDS             5664..6641
                     /gene="hrcA"
                     /locus_tag="NBI42_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCD1778450.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFSNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            6673..7299
                     /gene="grpE"
                     /locus_tag="NBI42_002052"
     CDS             6673..7299
                     /gene="grpE"
                     /locus_tag="NBI42_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCD1778451.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQTEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFISLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDHPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            7368..9200
                     /gene="dnaK"
                     /locus_tag="NBI42_002053"
     CDS             7368..9200
                     /gene="dnaK"
                     /locus_tag="NBI42_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCD1778452.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
BASE COUNT         3537 a         1264 c         1698 g         2775 t
ORIGIN      
        1 attaatgggc gatgctagta aaaataatga atctttacta ctaaaaaaat ataacttgcc
       61 ggagattgat attttaaaag taggacatca tgggagcaag acaagtagtt ctaaagaatt
      121 tatagagatg attaagccta aaataagttt gatttcttct ggaaagaaca atatgtatca
      181 tcttcctaac atagaagttg ttaaacgatt gcaaaggatt cgcagtcgca tttacaatag
      241 tcaacaaaac ggtcaagtta caattgactt agatgataat ttaaaagttg attcaaactc
      301 ttatggaaat gcaagtggtt tatagataca gaaacatgat ataatctatt gatgtgtcaa
      361 acgaatgaaa gggtgtaatg aatgagcgac aatattgtag ctatttatgg agatgtgcct
      421 gaattggttg aaaaacaaag tgcagaaatc atatcacaat ttttgaaaag tgatagagat
      481 gactttaact ttgtgaaata taatttatac gaaacagaga ttgcaccaat tgttgaagaa
      541 acattaacat tgcctttctt ttcagataaa aaagcaattt tggttaaaaa tgcatatata
      601 tttacaggtg aaaaatcgcc aaaagatatg gctcacaatg tagatcaatt aatagaattt
      661 attgaaaaat atgatggcga aaatttgatt gtctttgaga tatatcaaaa taaacttgat
      721 gaaagaaaaa agttaactaa aactctaaaa aatcatgcaa ggcttaaaaa aatagagcaa
      781 atgtctgaag aagaaataaa aaaatggatt caaagtaaat taaatgagaa tttcaaagat
      841 atcaaaagag atgcattaga tttatttatt gagttgacag gtattaactt taatattgtc
      901 tcacaagaga tagaaaagtt gattttattt ttaggtgata gaccaacaat caataagcag
      961 gatgttaacc aaattattaa tagaagttta gagcaaaatg tatttttact gactgaatac
     1021 attcagaaaa gaaagaaaga acaagcaatt catttagtaa aagatttaat aactatgaaa
     1081 gaagaaccta ttaaattact tgcactaatt acaagtaatt accgattatt ttatcaatgt
     1141 aagattctga gtcaaaaagg atatagtgga cagcaaattg ctaaaacaat aggtgtacat
     1201 ccatacagag taaaattagc gttaggacaa gtaagacatt atcaacttga tgaattattg
     1261 aatattatag atgcttgtgc ggaaactgat tataaactta aatcatcata tatggataaa
     1321 cagttaatgc tggaattatt cattctatct ttataaaaaa taaaagttca agcatgcgct
     1381 tgaactttta tttaaaaaga ttatttattt gcagtcatta attgtgattt aatacggtca
     1441 gctttgtttg aatgtattaa attactttga gcagctttgt ctactaactt aactgctaag
     1501 cttactaatt catttttatt atcagcgtta tttgaaacag ctgttttagc gtttttaact
     1561 gctgtacgca ttgcactctt ttgtgaaatg ttgcgtgctt cagctttttc agttgttttt
     1621 acacgtttaa ttgcagattt gatatttgcc atttctgtca cctcctaaaa gtgagttcat
     1681 cttatcaaaa ttttatttga ttacaacaag aaatatttta acaaaagtct aaccgttgtg
     1741 caatcattta tttgatgggg tcagcttaaa attttgcaga tttaataaat tgtgttggaa
     1801 acacaatcaa tacgttttat aattataagt acgtaattaa catattaaca tatcgcaagt
     1861 atgtatttaa ataaagattg ttataatttc aaagttcatc caagattatg gcgtttgcat
     1921 ttacctatta aaaacgttat tatatcaaag atgcgaaaga taatacgggt ttattttatg
     1981 aaagtgagaa ggataaaatg gataatgagc aacgcttaaa aagaagagag aatataagga
     2041 atttctcgat tatagcacat attgaccacg gaaaatctac attggctgat agaattttag
     2101 aaaataccaa atcagttgaa acaagagata tgcaagatca gttactagat tcaatggatt
     2161 tagaaagaga acgtggtatt acaatcaaat taaacgcagt acgtttaaag tacgaagcta
     2221 aagatggaaa tacttataca ttccatttaa tcgatacgcc tggacacgtc gattttacat
     2281 atgaagtgtc acgttctttg gcagcttgtg agggcgcgat tttagtagta gatgcggctc
     2341 aaggtatcga agcacaaaca ttagcaaatg tttatttggc attagataat gagttagagt
     2401 tattgcctgt tattaacaaa attgatttac ctgctgcaga acctgaacgc gtgaaacaag
     2461 aaattgaaga tatgattggt ttagaccaag acgatgttgt tttagcaagt gctaaatcta
     2521 acattggaat tgaagagata cttgagaaaa tagttgaagt tgtgccagct ccagatggtg
     2581 acccagaagc accactaaaa gcgttaatat ttgattctga gtatgatcca tatagagggg
     2641 taatttcatc gataagaatt gtagacggtg ttgttaaagc cggagataaa attcgaatga
     2701 tggccactgg taaagagttc gaagtaacag aagttggaat taatacacct aagcagcttc
     2761 cagttgatga attaacagtt ggtgatgttg gttatattat tgcaagtatt aaaaatgttg
     2821 atgattctag ggttggtgac acaatcacat tagctagtag acctgcatca gaaccattgc
     2881 aaggttataa gaaaatgaat ccaatggtat attgcggact gttcccgata gataacaaaa
     2941 attataatga tttaagagaa gcattagaaa aattacaatt gaatgatgca tcgttagaat
     3001 tcgagcctga atcatcacaa gcattaggtt ttggttatag aactggtttc ttaggtatgt
     3061 tacacatgga aataattcaa gaaagaattg aaagagaatt tggcattgaa ttaattgcaa
     3121 ccgcaccatc tgtaatttat caatgtattt taagggatgg ttcagaagtg acggttgata
     3181 acccagcaca aatgccagat cgtgataaaa ttgataaaat atttgagcca tatgttcgtg
     3241 caactatgat ggttccaaat gactatgtag gtgcagtaat ggaattatgt caacgtaaac
     3301 gtggacaatt tataaatatg gactatttag atgatattcg tgtaaatatt gtttatgaat
     3361 tacctttagc tgaagttgta tttgatttct tcgatcaact taaatctaat accaaaggat
     3421 atgcatcatt tgattatgag ttcatcgaaa ataaagaaag taatttagtg aagatggata
     3481 ttttattaaa cggtgataaa gtggatgcgc taagcttcat agttcataga gattttgcat
     3541 atgaacgtgg taaagcatta gttgaaaaac ttaaaacgtt aattccaaga cagcaatttg
     3601 aagtacctgt acaggctgca ataggacaaa aaattgtagc acgtacaaat attaaatcaa
     3661 tgggtaaaaa cgttttagct aaatgttatg gcggtgacat aagccgtaaa cgtaaattac
     3721 ttgaaaaaca aaaagcaggt aaagctaaga tgaaagcagt tggtaatgtt gaaattccac
     3781 aggatgcttt cttggctgta ttgaaaatgg atgacgaata atttttttaa aatcaattaa
     3841 caatttacaa tgaataaagt ttaataacta aaaagaggga gcctaggata aattaacgtc
     3901 ctgggcttta cgatgttata ttggcagtca tcgaccgagt tagaatgagc ttataagaat
     3961 ggggccccaa cacagagaaa ttggattccc aatttctact gacaatgcaa gttggcgggg
     4021 ccccaacaca gaggaattgg atttccaatt tctacagaca atgcaagttg gcggggcccc
     4081 aacacagagg aattggattt ccaatttcta cagacaatgc aagttggggt gggacaacga
     4141 aataaatttt gcaaaaatat catttctgtt ccactccctt atgcatgagt ttaactcatg
     4201 taattctatt tttaagtaca tattacctct ggctaatgtg taagagctac tacataataa
     4261 atcattagtg gttctttatt atttctatct cactatctct taacatttat aaatattaaa
     4321 atgaatcgat atattagaca atcattgatt aaacgttaaa gttaaaagta agaataattg
     4381 cagatagtcc aacaggatat agccgattgg ataaacagtc tgagaagcgg ggcattaaaa
     4441 tgacggtaca aagtgcatat atacatattc cattttgtgt aagaatatgt acatattgtg
     4501 atttcaataa atattttata cagaatcaac ctgtagatga gtacttagat gcactaatca
     4561 cagaaatgtc tacagcaaaa tataggaact taaagaccat gtatgtaggt ggtggcacac
     4621 caacggccct ttctattaat cagttggaaa gattacttaa agcaatacgt gatacgttta
     4681 caatcacagg cgagtataca tttgaagcaa atcctgatga gttaactaaa gagaaagtcc
     4741 aactattaga gaaatatgga gtaaacagaa tttcaatggg cgttcaaaca ttcaagccgg
     4801 agttattgtc tgttttaggt agaacgcaca atactgaaga tatttacact tcggtgttaa
     4861 atgctaaaaa cgcaggtatt aaatcaatca gtttagattt aatgtatcat ttaccgaaac
     4921 agacgattga agattttgaa caaagtttag atctagcttt agatatggat attcaacata
     4981 tttcgagtta cggcttaata cttgaaccaa aaacccaatt ttataatatg tatagaaaag
     5041 gtttgctcaa acttcctaat gaggatttag gtgctgacat gtatcagttg ctgttgtcta
     5101 agatagaaca atctcctttc catcaatacg aaatatctaa ttttgcatta gatggacatg
     5161 aatcagaaca taataaggtt tattggttta atgaggaata ttatggattt ggagcaggtg
     5221 caagtggtta tgtagatggt gttcgttata cgaatatcaa tccagtgaat cattatatca
     5281 aagctataaa taaagaaagt aaagcaattt tagtatcaaa taaaccttct ttgactgaga
     5341 gaatggaaga agaaatgttt cttgggttgc gtttaaatga aggtgtgagt agtagaaggt
     5401 tcaaaaagaa gtttgaccaa tctattgaaa gtgtctttgg tcaaacaata aataatttaa
     5461 aagagaagca attaattgta gaaaagaacg atgcgattgc acttacaaat cgagggaaag
     5521 tcattggtaa tgaggttttt gaagctttcc taataaatga ttaaaaaaat tgaaatttcg
     5581 agtctttaac attgacttac tttgaccaat ttgataaatt ataattagca cttgagataa
     5641 gtgagtgcta atgaggtgaa aacatgatta cagataggca attgagtata ttaaacgcaa
     5701 ttgttgagga ttatgttgat tttggacaac ccgttggttc taaaacacta attgagcgac
     5761 ataacttgaa tgttagtcct gctacaatta gaaatgagat gaaacagctt gaagatttaa
     5821 actatatcga aaagacacat agttcttcag ggcgttcgcc atcacaatta ggttttaggt
     5881 attatgtcaa tcgtttactt gaacaaacat ctcatcaaaa aacaaataaa ttaagacgat
     5941 taaatcaatt gttagttgag aatcaatatg atgtatcatc agcattgaca tattttgcag
     6001 atgaattatc aaatatatct caatatacaa ctttagttgt tcatcctaat cataaacaag
     6061 atattatcaa taatgtacat ttgattcgtg ctaatcctaa tttagttata atggttatcg
     6121 tattttcatc aggtcatgtt gagcatgtac atcttgcttc agacataccg tttagtaatg
     6181 ataaactaaa tacaatatca aattttgtta ctaacaaact aactgagttt aatcagaatt
     6241 tacaagatga tattgtctct tttgttcaat cagagcagga agaaatattt atcaataaat
     6301 tgttaaatac gatgaataat catatttcta atcaaagtaa tagcatttat atgggtggaa
     6361 aggttaaact tattgatgca ttgaatgaaa gtaatgtatc ttcaattcag cccattctac
     6421 aatatataga atcaaataga attgcagaat tattacaaga tatatcttca ccaaatatta
     6481 atgtaaaaat tggtaatgaa attgatgaca gcttaagtga tatttcaatt gtaacaagtc
     6541 aatatcattt tgatgaaacg ttaaaaggtc aaatcgctgt gattggacct acagctatgc
     6601 attatcaaaa tgtcattcaa ttattaaata gaatttggta atgatggatt agaaagtatt
     6661 ggaggacaga aaatgacaaa taaagacgaa tcagttgaaa aaaacactga atcaacagtt
     6721 gaagaaacaa acgtcaaaca aaatattgat gattcagttg aacaaactga agaaagtaaa
     6781 ggtcatttac aagatgaagc aatagaagaa acgtctgacg agaatgttat tgaagaaata
     6841 gatccaaaag atcaaaaaat taatgaactt caacaattag cagatgaaaa tgaagagaaa
     6901 tatttaaggc tctacgctga gtttgaaaat tataagcgta gaattcaaaa agaaaatgaa
     6961 ataaataaaa catatcaagc acaacgtgtg ttaacagata ttttaccagc aatagacaat
     7021 atagaacgtg cacttcaaat tgaaggtgat gatgagactt ttatatctct tcaaaaaggt
     7081 gtacaaatgg tgcatgaaag tttgattaac gcactaaaag ataatggtct tgaagttatt
     7141 aaaactgaag gtgaagcatt tgatccaaat attcaccaag ctgtagttca agatgatcac
     7201 cctgattttg aatctggcga aatcactcaa gaactacaaa aaggatacaa gcttaaagat
     7261 agagtattaa gaccatcaat ggtcaaagta aaccaataac ttaaatttgg cgaaacgaca
     7321 ttgtttaaaa tcaaattaat ttaatgatta aatggaggaa ttttattatg agtaaaatta
     7381 ttggtataga cttaggtaca acaaattcat gtgtaacagt attagaaggc gatgagccaa
     7441 aagtaattca aaatcctgaa ggttcacgta caacaccatc tgttgtagct ttcaaaaatg
     7501 gagaaactca agttggtgaa gtagctaaac gtcaagctat tacaaaccca aatactgttc
     7561 aatctattaa acgtcatatg ggtactgatt ataaagtaga tattgaaggt aaatcataca
     7621 caccacaaga aatctcagct atgattttac aaaacttaaa aaatacagct gaaagctatt
     7681 taggtgagaa agttgacaaa gctgtaatta cagtacctgc atactttaac gacgctgaac
     7741 gtcaagcaac taaagatgct ggtaaaattg ctggtttaga agttgagcgt atcattaatg
     7801 aaccaacagc tgcagcatta gcatatggtt tagacaaaac tgacaaagat gaaaaagttc
     7861 ttgtttttga cttaggtggc ggtacatttg atgtatctat cctagaatta ggtgacggtg
     7921 tattcgaagt actatcaaca gccggtgaca acaaacttgg cggtgatgat tttgaccaag
     7981 taattattga ctacctagtt gcagaattca aaaaagaaaa tggcgtagac ttatctcaag
     8041 ataaaatggc attacaacgt ttgaaagatg ctgctgaaaa agctaaaaaa gacttatcag
     8101 gtgtatcaca aactcaaatc tcattaccat ttatctcagc tggtgaaaac ggtccattac
     8161 acttagaagt aaacttaact cgttctaaat ttgaggaatt atcagattca ttaattagaa
     8221 gaacaatgga acctacacga caagcaatga aagatgctgg cttaacaaac tcagatatcg
     8281 atgaagttat cttagttggt ggatcaactc gtattccagc agtacaagaa gctgtcaaaa
     8341 aagaaatcgg taaagaacct aacaaaggtg taaacccgga cgaagtagtg gcaatgggag
     8401 ctgcaatcca aggtggcgtt atcacaggtg acgttaaaga cgtagtatta ttagacgtaa
     8461 caccactatc tttaggtatt gaaattttag gtggacgtat gaatacgtta attgaacgta
     8521 acactacgat tcctacatct aaatctcaaa tctattcaac agcagtagat aatcaaccat
     8581 cagtagatgt acacgtattg caaggtgaac gtccaatggc tgcggataat aaaacacttg
     8641 gtagattcca attgactgat attccaccag ctgaacgtgg taaacctcaa attgaagtaa
     8701 cgtttgatat cgataaaaac ggtattgtaa atgtaactgc aaaagactta ggtacaaata
     8761 aagaacaaag aattacaatt caatcaagtt cttcattatc agacgaagaa atcgaccgta
     8821 tggtaaaaga tgctgaagtt aacgctgaag cagataaaaa acgtcgtgaa gaagtagact
     8881 taagaaacga agctgacagc ctagtattcc aagttgaaaa aactttaact gatttaggcg
     8941 aaaatatcgg tgaagaagat aaaaaatctg ctgaagagaa aaaagacgct cttaaaactg
     9001 ctttagaagg tcaagatata gaagatatta aatctaaaaa agaagaactt gaaaaagtga
     9061 ttcaagaatt atcagcaaaa gtatatgagc aagcggctca acagcaacaa caagcacaag
     9121 gtgctaatgc tggtcaaaac aacgatagta ctgtagaaga tgctgaattt aaagaagtaa
     9181 aagacgacga caaaaaataa ttaaacaata caattagaat gttgtcatga tctgaaaagt
     9241 caaagtcaat agaacattgg ctttggcttt ttat
//
LOCUS       DAHHRR010000100         9101 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.100, whole genome shotgun sequence.
ACCESSION   DAHHRR010000100 DAHHRR010000000
VERSION     DAHHRR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.100"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(521..596)
                     /locus_tag="NBI42_002054"
                     /pseudo
     CDS             complement(521..596)
                     /locus_tag="NBI42_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(804..1400)
                     /gene="recR"
                     /locus_tag="NBI42_002055"
     CDS             complement(804..1400)
                     /gene="recR"
                     /locus_tag="NBI42_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCD1778453.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1407..1724)
                     /locus_tag="NBI42_002056"
     CDS             complement(1407..1724)
                     /locus_tag="NBI42_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCD1778454.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1814..3511)
                     /gene="dnaX"
                     /locus_tag="NBI42_002057"
     CDS             complement(1814..3511)
                     /gene="dnaX"
                     /locus_tag="NBI42_002057"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCD1778455.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALNHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3580..4104)
                     /locus_tag="NBI42_002058"
     CDS             complement(3580..4104)
                     /locus_tag="NBI42_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1778456.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4243..4344)
                     /locus_tag="NBI42_002059"
     CDS             complement(4243..4344)
                     /locus_tag="NBI42_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778457.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSEIDAQFE"
     gene            complement(4414..4683)
                     /gene="ffs"
                     /locus_tag="NBI42_002060"
     ncRNA           complement(4414..4683)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="NBI42_002060"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4745..5473)
                     /gene="treR"
                     /locus_tag="NBI42_002061"
     CDS             complement(4745..5473)
                     /gene="treR"
                     /locus_tag="NBI42_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCD1778458.1"
                     /translation="MVKQKKFMKIYEALKEEILNEQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRTK"
     gene            complement(5498..7138)
                     /gene="treC"
                     /locus_tag="NBI42_002062"
     CDS             complement(5498..7138)
                     /gene="treC"
                     /locus_tag="NBI42_002062"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCD1778459.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKNSDKIGKE
                     FYTDGPRVHEFLYELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTTGKPWIDIPENYHQVNVRQALQ
                     NKDSIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLTCEGRVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7202..8629)
                     /gene="treP"
                     /locus_tag="NBI42_002063"
     CDS             complement(7202..8629)
                     /gene="treP"
                     /locus_tag="NBI42_002063"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCD1778460.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGLVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSGISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
BASE COUNT         2883 a         1967 c         1215 g         3036 t
ORIGIN      
        1 atagttacgt ttttggaatt aacgttgaca tattgtcatt cagttttcaa tgttcatttt
       61 tcttaccgac aagaattaat tatacatttt attaacattt aagtcaacaa ctttttaaaa
      121 ttaattttcg ttagtaattc aagttatttc ttaagttgag ttgttttgtt tgcctcaaca
      181 aataaatact atacaggctt tcaaactgct taacaacagt aaaaattaca cctttgccat
      241 ctattctgaa cactattatc ctaaaaatcg aactttaaaa aaacccgctt ataaatagta
      301 cgttttttag aacaagaaat agatttttct ctaaacatat caatcgaaat aaccgcaaaa
      361 cagaacgtta tttttttacc agaaattaaa aataaattta caagttttaa ataatctctc
      421 aaaaccccgc tttacaggct gatttaataa gctttagacc aaagaagtac aactactaag
      481 ccagcatcta tcatttcaat tacaaaagaa tttcatttgc ctagtcatta accatcaacc
      541 gcatttatca aattaatagc accaacacac cacaacagta ttcctacttc gatgattcaa
      601 ttcgaatcat tactattaga agtaacaaat cacaaccaac gatttgcttc tttaagatta
      661 cccatcctca gtaaaactag ctaaaccatg ccaccaacac accacaaata aagtagtaca
      721 ccacaccata aaaaaagcca caatgattac actgtgactt ttcttactat agtattaaat
      781 tcaaaaatgt ttaatagaag acattacatt tctgttctac ctgcgattgc tttagataat
      841 gttacttcgt cagcatactc taaatcgcca cctaccgata acccttgtgc taatctcgtc
      901 actttgatac ctataggctt aactaatcta gaaatataca tggctgtaga ttccccctct
      961 aagttcgggt tcatagctaa gattaattcg ctaacttcat cgtttttcaa gcgttcaatc
     1021 aatgaaggaa tattaatatc ttctggtcca attccatcca taggcgaaat agacccatgt
     1081 aaaacgtgat ataaaccttt gtattctctc attttttcca tagctatgac atctttgtca
     1141 tcttccacaa cacaaataac tgaacgatct ctttgcttat cttcacaaat ataacatgga
     1201 tcattttcag taatgtgacc acatacgcta caatatgtta attctctttt aacatctact
     1261 aatgctttgg caaactgaac aacatcgtct tctttcatat ctaaggtatg aaaagccaga
     1321 cgttgggctg tctttggacc aatgcctggc aatttcataa agctatcaat aagctttgat
     1381 ataggttctg gataatgcat ctataatcac attccaggga tgtttaagcc ttgagtatgt
     1441 ttacctaaac gttcttgagt aagctcatca gctttattca tcgcttcatt agtagctgct
     1501 aacactaagt cttgtagcat ttcaatatca tctgggtcta cagcttcttc tttgatttca
     1561 acgtcgacaa cttctttatg accagttaca gtaactgcaa ccatgccacc gccagctgtt
     1621 cctacaatac gctcttcttt aagtttttct tgttcttgag ccattttctt ttgcattttt
     1681 tgcatttgtt tcatcatttg ttgcatgttt ccgccaccgc gcatattcca tttcctcctt
     1741 gaaaatcaat aaatatttat caataaaatg atgtttcttt cattatacat acgattatat
     1801 cgcttgtcat gtatcactct tcatctatca catgtacagt ttcttcaccg aaaagatctt
     1861 ttgctttttg agcaatatct gtttgttgtg cttgttgctt tggcatatca tcgccttcgt
     1921 ttttacgatt ttgtaaatac tccgttcgaa ctctttgcca ttgatctgat ggtacaccaa
     1981 caactttaac gtttttatta acgatattac atacaacact ttctatacta ctacgtttct
     2041 cgtcgtcttt attgacgatt tcacaatgga tctcttcctc aaatttcaca agtacgtgat
     2101 cttcacttgc cgccacaggt tccgaatttt gcaataaact aacgagtgat tttttatcat
     2161 tgtttttggc atgatcaatc acttcttgcc aatgatcttt caacaatttg atatctgcct
     2221 tattcgcttt atctagcact tttgcaattt gttgcattga aaatgcattt ttagattttt
     2281 gtatacctct cgcaggcttt ttcgaagatt tttgaacagg agcgacactc actccttgtg
     2341 cttttagcgt ttttagttct tgctctaact gttccatacg ttgcaacaat acatctgtgt
     2401 ttggcgatga agcaatttgt gctggttcag ctacattcgc aatcacttgt ggttgaccct
     2461 taatctgctc agctaatttt actaacaaca cttcaaaatg aacgttttga ttcacactaa
     2521 aacgaatcga cactaatgta tcattaataa gatcaatcat ttgatataac atatctaatt
     2581 ctaagttcat cagtgctcga tactcagtat ctttctcaga tgttttattc ataatcgtat
     2641 ctctgacaaa ataaatcata tcatttatta ggcgattcac ttctttacct tctgttataa
     2701 actgatggta ttttttaaaa gatgcttgta catcaccttg tacaatatca tcaaacaagt
     2761 gattcaacgc ttcatcatga acgctacccg taacatttag ggcatcttgt aatgtcaatg
     2821 tgccatcgcc gaaagcaata gcctgatcca taatacttaa tgcatcacgc ataccccctt
     2881 cagacgcttt agcgataaat gccaaggctt catcttcaca ttcaatttgt tgtgcatctg
     2941 ctacaaattt taaacgttca acaatttgat ctaagctaat tgctttaaaa tcaaaacgtt
     3001 gtgccctaga aatgattgtt ggagggattt tatgtggttc tgtcgttgcc aatataaaaa
     3061 tagcgtgtgc tggaggttct tctaacgtct ttaaaagggc attaaaagca cctgttgtta
     3121 gcatgtgcac ctcatctata atataaactt tatatttcga ttcacttggt gcatatttaa
     3181 ctttgtctct aatatttctt atttcatcaa caccattatt actagcagca tcaatttcta
     3241 tcacatctga attagtcccc tgcgtaatgc ctttacaaat atgacattca ttacaaggtt
     3301 ctccatcagt gctatttaaa cagttgattg ctttagcaaa cactttggca atactcgttt
     3361 tccccgtacc tctcggacca ctaaaaatat atgcatgcga ctgtttttct ttcgaaatcg
     3421 cattgcgcaa tgtcttcgtg acatgttctt gtccgacgac atcctcgaaa ctttggggtc
     3481 tgtacatacg atataaggct tgataattca agttagcacc tccataaaca attacctttc
     3541 attatagcat gataatacct ttacttctta aatgaacaat taataaaaat gttctggaaa
     3601 ttttacgatt ccgtgtggtg catccgtcaa attatcccat aaatatttta caagtaacgg
     3661 ggcatcacca gattctaatt taatgtcatg ctcagcagca ttttgataac ccaacttttc
     3721 aaagtagtca aaacaatggt ctacaacaac cgtactatac tcttgcgctt ttgcacgttc
     3781 ttctactgct tgaaccaaac cacgacctaa tttttgtcca cgtaattcag gatgaactga
     3841 taaagaggca atcgccaaac cataatacgt cttatcatca ctattaattt ctacttcaat
     3901 taataaaacg tgtccaacga catcgttatt ttcatttttc gctattactt ctaattcaaa
     3961 attatagcaa ggagatttcc ttaaatgttt cactttcgca cgtgcttgcc aactcgtttc
     4021 aggattatca tcaaaacttt cttcaatact atttaaagat ttatcataat ctaactctgt
     4081 taaagtactt aaatatattt gcatatgtcc tccgtaggga tttgattgtc aataatcata
     4141 acgtatctca ttaataattc tattgtaagg tactccccct catttcacca tcatttcttt
     4201 atcatcaaat aataacttct tactttttat atccgtaaaa aattattcaa actgcgcatc
     4261 gatttcactt gaatcaaact tcacatataa aaaagctact tcccacaaac atgttccacg
     4321 tataatacgc tgaattgtct tcaagaaagt agcttctata attatatatt ttcaactctt
     4381 aataatcggt ttaatatttt aaaaaataaa aaaccgtgca cctaagcatc gaacgtaatt
     4441 cacgaacgta accaaagtcg ttagctaaat ctcggctacc ctacggcaca tatgatgatc
     4501 cacttaatgc tgcttccgtc aggacctgac atggttcatg ggttcatatt gcataggacc
     4561 gaaatcttca aacactacgt gctttgggca gacttcgcaa aaatacggcc tcaacaaagg
     4621 aattaagcct cgcataaagc ggatttcgag tacagggaac cgctacctcc ccacctagca
     4681 cggcaagata tatattacta tattttaata gttaattgca agtataaatc atttatatca
     4741 ttgtttactt tgtacgacgt cttgagaagt cattaaattt aaattcattt gcaagatgtt
     4801 ttgaaatatt atattgaaac ggcattgtat tttctaaata cacaatactt cgaactgttg
     4861 ctgaataagc caccgataca tccccaaaca attgatatgc ttgttcatca aacggttcaa
     4921 aagtaataga cttacttgaa taactaatat taagatttaa taccttttcc aaataatcat
     4981 aaatagaatc actcgcaaca tcattaccta tatctgaaac aatcgacttt agaaaataat
     5041 cttcatcaac aatcttcaca tgttggttaa gccgacgagt tctaacaata tgaatgagtg
     5101 attcactaga attgatgttt aaagcatgtt gaacttctgg aacttcatgc gcttcaacaa
     5161 cctcattcac aacaacttca gttaaatatg cgacacccat ttcttcttgc atttctttaa
     5221 aactaacaag ttcagaaaat ggaaactctg taacctcctg ataaatgaca agtgaccctt
     5281 taccatgaat cttttgaatc ataccgtcta gtgccaacaa atctaatgcc ttacgcacgg
     5341 tctctcgaga tgactggtac aattgcacca aatcatgttc agacggaatt tgttcaccat
     5401 attgaatctg ctcgtttagt atctcttctt tcaacgcctc ataaatcttc ataaatttct
     5461 tttgcttcac catataaacg catccactca atttatttta cgcgtttgtt tcgattacaa
     5521 ttgcaccaaa cccgcttatc gtattatttt ccactgtacc tgtttgaatc acaacacgac
     5581 cttcgcacgt caatccttct ggcaaatcaa cagccgatgc tgagaaattc gcaattacta
     5641 gccattgttg attcttataa tgacgttcat aaacaaataa atgatcatga tccataaaac
     5701 gtggcacaat gtctccatac gtaataatat catgcgtatg tcttaattgt attaattttt
     5761 gatacgtgta gaaaatagaa tctttattct gaagtgcttg tctaacgttg acttgatgat
     5821 aattttctgg aatatcaatc caaggcttac cagttgtaaa tccagcatta acatcatcac
     5881 tccattgcat aggcgttctc gaattgtctc gtgacttctg tcctaaaatc gctaacacat
     5941 ccgcttcagc atgcccttca cttaacaact gatggtaggc attaatcgat tcaacatcac
     6001 gatattgtgc tattgatgta aaatgtgggt ccgtcatacc aatttcttca ccttggtaaa
     6061 tatatggcgt cccttgcaac atatgcagtg cgatagctaa cattttagca ctttgtatcc
     6121 tcatctcttc cgacgtatca tcaccaaatc tagacactac ccgtggctga tcatgattac
     6181 accagaaaat cgcgttccat ccgccaccgt cataaatacc tcgttgccat tgcatcagaa
     6241 tttcctttaa cttatgaaaa tcaagcttcg catttgtcca cttttcacca tcaacataat
     6301 caacctttag atgatgaaaa ttaaaaacac tattcaattc ttggcgttct ggctgtgtat
     6361 acttaataca attttcaatc gtcgtcgaag acatttctcc tacagtcatc atgtcagtgt
     6421 taccaaacgt ttgacgattt aattcataca gaaactcatg cactctagga ccatccgtat
     6481 aaaattcttt acctattttg tcagagttct taaattcacc tttagaaatt aagttaatga
     6541 catcaaatcg aaaaccatca acgccgaagt ctatccaatg attgacaatg cgatataacg
     6601 attgacgtac ttccggatta tcccaattta aatcagcttg actgacatca aataaatgta
     6661 aataatattc atctgtctca gaatcatact tccatgcatt accaccaaat ttagaatgcc
     6721 aatttgttgg cggtccgtct tcagatgatc tgaaaaagta ataatctcta taagggttgt
     6781 ctttagattt acgggcttct ttaaaccatt catgctccgt agacgtatga ttaatgacaa
     6841 tatctaacat cactttcaag tctttttgat gagcaacttt gattaacttt tcaaaatcat
     6901 ccatcgttcc aaagtcttca ttgatttcta aataattgct gatatcatag ccattatcat
     6961 tcatcggtga ttcatacact ggtgttaacc aaatataatc aacacccaat aacttgatat
     7021 aatccaattt ttctataatt ccgttaatat ctcctatacc attccccgtc gtatcattaa
     7081 acgacttagg ataaatttga tatacaacgg attttctcca atctatttct ttcgacacac
     7141 aattaccacc ttataacgta ataaaggacg tccaaataac gaacgcccct tttttatttt
     7201 attaatcttc aacaatttct ttcgcttttt gtttactaaa atgagacatc acaattgtta
     7261 gtatacatgg tacaacaata gcaatagctg tcacaataag atatactggc caaaattctt
     7321 tttgaattga aatgaatgct ggcacaccac caacaccaac ttttccaagt acgttattca
     7381 taccaacgat tgcccccaat acacaagacg ttgatatcgc agcgataaat ggatatttca
     7441 gaggtaagtt cacaccaaac atagctggtt cagtaacacc taacatacca gaaataccag
     7501 atgttaatgc caagccttct tctttaacca ttttacgacg tttatagaca aaccatgctc
     7561 caaatgctgc agacccctga caaatatttg aaatcgcaac gattggccat aagtacgtac
     7621 cacctaagct gctacccatc aattggaaat ctactgctaa aaacatatga tgcaagccag
     7681 taattacaag cggtgcatat agcaaaccat aaattgcgcc acctaaccaa ccagcatgtt
     7741 ggaaaataaa tgtaacacca gatgtaatac ctgtaccaat caataatgca actggtccaa
     7801 tgataataaa tgctaaaaat ccagtaacta aaagcgctac gggtccaacg accaacattt
     7861 ttatcgaatc gtgaacgact ttatttaatc ctttttcaat tttagctaga acataagctg
     7921 caattaaaac aggcaacact tgaccttggt aattcaactg cttaatctct aagccaaata
     7981 agttccacgt cggaatattc ccttttgcca aatcatactg agatactaat tgcgggtgca
     8041 ttaaaatcaa acctaagact aagcctagaa tcggactacc accaaataca cgcatactac
     8101 tccaaccaat taatgctggt aagaaaataa atgccgtact cgcaatcaca ttaatgatgt
     8161 ttgaaatatc agcaatttgt ggatacatct caataagtgc ttttggacca aataaacctt
     8221 tcattgtaag taaattattg atccccatta acaaaccagc tgtcacaatc gcaggtaata
     8281 ttggtataaa aatatcccct aacaatttga tcaaacgttg tactggattc cctttttgcg
     8341 cagctgcttg tttcgcttca tctttcgaag cttcttgagc acctgtttca tcaataaact
     8401 gcttatacac ttcatcgact gttcctggac caatgacaat ttgatattga tggtctgctt
     8461 tgaactgccc cttgaccaat gcgttattac ttaatgcgtc tttatcaact ttgctttcat
     8521 ctttaagcac taaacgtaat cgtgttacac aatgcgttgc agcttcaaga ttttcttttc
     8581 ccccaatagc agttacgatg gcttttacat cttctctttt tacagccata tatttcactc
     8641 ccttaatgtc ttaaaacgag tataacttgt ctagacaagt tatgtaaagg ctttcaacaa
     8701 aatattttta ttttgttaca attatctggt gtagccattt cagaaaatca atgagccata
     8761 acgacatatg tagcaataac ccctcgctac aattgtgtta ttaacaatac ctcaccacgc
     8821 tctcgccatt ataaagtgta aatgcatggc caactcctaa attgaaaaat gcatatctac
     8881 aaatagcaaa cttgcccaca caagccatcc atcatatttc acaacacaaa aaagacattt
     8941 agctatgtat attgcacaag ccaaatgccc ttccaatctc aatgccttat catttctatt
     9001 atcaaaaaat acgagtcatt caaaggcata taaaaaaggt aaacactcaa actttgagta
     9061 cttacctttt ttatatatag tatttaatat aaatattgat g
//
LOCUS       DAHHRR010000101         9098 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.101, whole genome shotgun sequence.
ACCESSION   DAHHRR010000101 DAHHRR010000000
VERSION     DAHHRR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.101"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            71..883
                     /gene="tagG"
                     /locus_tag="NBI42_002064"
     CDS             71..883
                     /gene="tagG"
                     /locus_tag="NBI42_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HCD1778461.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMVHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            982..2085
                     /locus_tag="NBI42_002065"
     CDS             982..2085
                     /locus_tag="NBI42_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1778462.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSTQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLALYVPT
                     YREDKADNRAIDKAYFETCLPGYTLINKLHPSIEHSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEETYDKVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            2082..3143
                     /locus_tag="NBI42_002066"
     CDS             2082..3143
                     /locus_tag="NBI42_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD1778463.1"
                     /translation="MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTNPHDSDAFMVINPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTITEQPDLLTMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNNE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMCMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKRF
                     LIQLKGRKFAHRTKSMLKR"
     gene            3206..3604
                     /gene="tagD"
                     /locus_tag="NBI42_002067"
     CDS             3206..3604
                     /gene="tagD"
                     /locus_tag="NBI42_002067"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCD1778464.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVKKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(3721..5016)
                     /gene="pbp4"
                     /locus_tag="NBI42_002068"
     CDS             complement(3721..5016)
                     /gene="pbp4"
                     /locus_tag="NBI42_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HCD1778465.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQAANSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     IENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWAEHPLFTIIGGACLVAGLALIVHMIINRLFRKRK"
     gene            5437..7164
                     /locus_tag="NBI42_002069"
     CDS             5437..7164
                     /locus_tag="NBI42_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1778466.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            7523..8752
                     /locus_tag="NBI42_002070"
     CDS             7523..8752
                     /locus_tag="NBI42_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD1778467.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            9007..>9098
                     /locus_tag="NBI42_002071"
     CDS             9007..>9098
                     /locus_tag="NBI42_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778468.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
BASE COUNT         3123 a         1284 c         1623 g         3068 t
ORIGIN      
        1 caacaattat tataatacta tttagttaag tagagaaata gttaaacatt tgaaagtgtg
       61 gtttaatgga atgtcagcaa taggaacagt ttttaaagaa catgtaaaga acttttattt
      121 aattcaaaga ctggctcagt ttcaagttaa aattatcaat catagtaact atttaggtgt
      181 ggcatgggaa ttaattaacc ctgttatgca aattatggtt tactggatgg tttttggatt
      241 aggaataaga agtaatgcac caattcatgg tgtacctttt gtttattggt tattggttgg
      301 tatcagtatg tggttcttca tcaaccaagg tattttagaa ggtactaaag caattacaca
      361 aaagtttaat caagtatcga aaatgaactt cccgttatcg ataataccga catatattgt
      421 gacaagtaga ttttatggac atttaggctt acttttactt gtgataattg catgtatgtt
      481 tactggtatt tatccatcaa tacatatcat tcaattattg atatatgtac cgttttgttt
      541 tttcttaact gcctcggtga cgttattaac atcaacactc ggtgtgttag ttagagatac
      601 acaaatgtta atgcaagcaa tattaagaat attattttac ttttcaccaa ttttgtggct
      661 accaaagaac catggtatca gtggtttaat tcatgaaatg atgaaatata atccagttta
      721 ctttattgct gaatcatacc gtgcagcaat tttatatcac gaatggtatt tcatggttca
      781 ttggaaatta atgttataca atttcggtat tgttgccatt ttctttgcaa ttggtgcgta
      841 cttacacatg aaatatagag atcaatttgc agacttcttg taatatattt atatgacgaa
      901 accccgctaa ccattaataa atggaagtgg ggttcatttt tgtttataat ttaagtaaat
      961 aacatattaa gttggtgtat tatgaacgtt ttaataaaga aattttatca tttggtagtt
     1021 cgaatacttt ctaaaatgat tacgcctcaa gtgattgata aaccgcatat cgtatttatg
     1081 atgacttttc cagaagatat taagcctatc atcaaagcat taaataattc gtcgtatcag
     1141 aaaactgttt taacaacacc aaaacaagca ccttatttat ctgaacttag cgacgatgtt
     1201 gatgtgatag aaatgactaa tcgaacattg gtaaaacaaa ttaaggcttt gaaaagcacg
     1261 cagatgatta ttatcgataa ttattaccta ttactaggtg gatataataa gacttctaat
     1321 caacacattg ttcaaacgtg gcatgcaagt ggtgcattaa aaaactttgg tttaacagat
     1381 catcaagtcg atgtgtctga caaggcaatg gttcagcagt atcgtaaagt ttatcaagcg
     1441 acggattttt acttagtggg ttgtgaacaa atgtcacaat gttttaaaca gtctttaggt
     1501 gcaacagaag agcaaatgtt gtattttggg cttccgagaa ttaataaata ttacacagct
     1561 gatagagcaa cggttaaggc agagttaaag gataaatatg gcattacaaa taagttggca
     1621 ttatatgtac caacatatag agaagacaaa gcagataata gggctattga taaagcttat
     1681 tttgaaacat gtttaccagg atatacattg attaataaat tacatccatc aattgaacat
     1741 tcagacattg atgacgtatc ttcaatcgac acgtctacat taatgttaat gtcagatata
     1801 attattagcg actatagttc gctgccaata gaagctagct tgttagatat tccaactata
     1861 ttttatgtgt atgatgaaga aacatatgat aaggtgagag gcctgaatca attttacaaa
     1921 gcaataccgg atagctacaa agtgtatact gaagaagatt taataatgac gatacaagaa
     1981 aaagaacatc tattaagtcc gttatttaaa gattggcata agtataatac tgataaaagt
     2041 ttacatcagc tcacagaata tatagataag atggtgacaa aatgaggctt acgataatca
     2101 tacctacatg taataatgag gcaacaattc gacaattgtt aatatctatt gagagtaaag
     2161 aacactatag aatcctttgt attgatggtg gttctactga tcaaacaatt cctatgattg
     2221 aacggttaca aagagaactc aagcatattt cattaataca attacaaaat gcttcgatag
     2281 ctacgtgtat taataaaggt ttgatggata tcaaaatgac aaatccacat gatagtgacg
     2341 catttatggt tataaatcca acatcaatcg tattgccagg taaattagat aggttaactg
     2401 cagctttcaa aaataatgat aatattgata tggtaatagg gcagcgagct tacaattacc
     2461 atggtgaatg gaaattgaaa agtgctgatg agtttattaa agacaatcga atcgttacaa
     2521 taacggaaca accagatttg ttaacaatga tgtcttttga cgggaagtta ttcagtgcta
     2581 aatttgctga attacagtgt gacgaaactt tagctaacac atacaatcac gcaatacttg
     2641 tcaaagcgat gcaaaaagct acggatatac atttagtttc acaaatgatt gtcggagata
     2701 acgatataga tacacatgct acaagtaaca atgaagattt taatagatat atcacagaaa
     2761 ttatgaaaat aagacaacga gtcatggaaa tgttactatt acctgaacaa aggctattat
     2821 atagcgatat ggttgatcgt attttattca ataattcatt aaaatattat atgaatgaac
     2881 acccagcagt aacgcacacg acaattcaac tcgtaaaaga ctatattatg tgtatgcagc
     2941 attctgatta tgtatcacaa aatatgtttg acattataaa tacagttgaa tttattggtg
     3001 agaattggga tagagaaata tacgaattgt ggcgacaaac attaattcaa gtgggcatta
     3061 ataggccgac ttataaaaga ttcttgatac aacttaaagg gagaaagttt gcacatcgaa
     3121 caaaatcaat gttaaaacga taacgtgtac attgatgacc ctaaactgca atcctatgat
     3181 gtgacaatat gaggaggata actaaatgaa acgtgtaata acatatggca catatgactt
     3241 acttcactat ggtcatatcg aattgcttcg tcgtgcaaga gagatgggcg attatttaat
     3301 agtagcatta tcaacagatg aatttaatca aattaaacat aaaaaatctt attatgatta
     3361 tgaacaacga aaaatgatgc ttgaatcaat acgctatgtc gatttagtca ttccagaaaa
     3421 gggctgggga caaaaagaag acgatgtcaa aaaatttgat gtagatgttt ttgttatggg
     3481 acatgactgg gaaggtgaat tcgacttctt aaaggataaa tgtgaagtca tttatttaaa
     3541 acgtacagaa ggcatttcga cgactaaaat caaacaagaa ttatatggta aagatgctaa
     3601 ataaattata tagaactatc gatactaaac gataaattaa cgtaggttat tataaaataa
     3661 atataaaacg gacaagtttc gcagctttat aatgtgcaac ttgtccgttt ttagtatgtt
     3721 ttattttctt tttctaaata aacgattgat tatcatatga acaattagtg ctaatccagc
     3781 gacaaggcat gcaccaccaa tgatagtgaa taatggatgt tctgcccaca tacttttagc
     3841 aacagtattt gccttttgaa taattggctg atgaacttct acagttggag gcccataatc
     3901 tttattaata aattctcttg gatagtccgc gtgtacttta ccatcttcga ctacaagttt
     3961 ataatctttt ttactaaaat cacttggtaa aacatcgtaa agatcatttt caatataata
     4021 tttcttacca tttatccttt gctcaccttt agacaatatt tttacatatt tatactgatc
     4081 aaatgaacgt tccattaatg cattccccat catattacgt tgcttctcgc caccaaggtt
     4141 tttatagtct cctgcaccca tgataacttg attaattcta aatttacctc gtttagtagt
     4201 aatcgtatgg ttgtaatttg ctgtatcact tgatccagtt tttaaaccat ctgtaccagg
     4261 caaactcatt tttgcacctt ctaatgaaaa gttgaatgtg taatacgtaa ctgcatgcgt
     4321 tgttggtgct aactgctttg taaagtctaa tattttaggt gtctctttaa tcacgtgtaa
     4381 atctaaaatg gcatagtctc tagcagtcgt tacagtacgt tcttggtctt tatactttgt
     4441 tggtgcaaat gtacgtaatc ttgaattttc agcacccgtt ggattgacga aatgtgtatt
     4501 tttcattccg atagctttag ctttgttatt cattaaatca acgaaatcgc tggtgttttt
     4561 tgataccttc ttagctaaaa ttaatgccgc ggcattgcta gaattagata ctgtaatttg
     4621 taataggtct gcgattgtcc atacttgtcc aggatatagc ttcgtattac tcagctcagg
     4681 tagtgtagac ataatatatt ctttgttcgt cattgtgaca gtgtcatcaa gtgaaagctg
     4741 ccccttattt acagcttcca atgttaagta cattgtcatt aatttagtca tagacgctgg
     4801 attccactta gtatcgatat tgtattgata cagtaattgt ccagtttgac ttacattaac
     4861 agcactcgtc ggttcgtatg cagccgacaa acctgcataa ccatattgat ttgctgcttg
     4921 tacaggggtt acgtcactgt tagcagcttg tgcatatggt gtcataatac ttaatgttaa
     4981 acataaaatg atgataatag atattaaatt tttcataaag cgttaatctt cccttttcca
     5041 attcttaaat attccctaaa agcaatggtc attcctaatt acggaaatca ttgctaattc
     5101 acttaacctt aattaaattg ttgaaaataa agttttctgc agttaatttg aaaaataatg
     5161 caaatatatt acgtgcgtag ctaaaggtgt tataatgttt gtacgaagag caaacttact
     5221 caaaagcgat taattatcat gttttaatat aaagactttg agaagttatt acaaaaaatg
     5281 caatagaaat attctatcat ataaatgtta tgagcggtat tttggggcaa tactttattt
     5341 gatttttaaa gttttgttgg gagaaagtat atgatagaaa tgcatgtatc tatctaaatg
     5401 aattaactac aaatttcaaa cagaagaggt aaaactatga aacgagaaaa tccattgttt
     5461 ttcttattta aaaaactatc atggccagtg ggtcttatcg ttgcagctat cactatttca
     5521 tcactaggga gcttaagtgg actattagtg ccactgttta ctggacgaat tgtagataaa
     5581 ttttccgtga gccatatcaa ttggaatcta atcgcattat ttggtggtat ctttgtcatc
     5641 aatgctttat taagcggatt aggtttatat ttattaagta aaattggtga aaaaattatt
     5701 tacgcgatac gctcagtttt atgggagcat atcatacaat taaaaatgcc attctttgac
     5761 aaaaatgaaa gtggtcaatt aatgagtcga ttaactgacg atacgaaagt gataaatgaa
     5821 tttatttcac aaaagctacc taacttatta ccatcaatcg ttacattagt tgggtcacta
     5881 attatgttat ttattttaga ttggaaaatg acattattaa catttataac gataccgata
     5941 tttgttttaa ttatgattcc tctaggacgt attatgcaaa agatatcgac aagtacacaa
     6001 tctgaaattg caaactttag tggtttgtta gggcgtgtcc taactgaaat gcgtcttgtt
     6061 aaaatatcaa atacagagcg acttgaatta gataatgcac ataaaaattt gaatgaaata
     6121 tataaattag gtttaaaaca ggctaaaatt gcggcagttg tacaaccaat ttcaggtatt
     6181 gttatgttgc taacaattgc aattatttta ggttttggtg cattagaaat tgcgactggt
     6241 gcaatcactg caggtacatt aattgcaatg atattttatg ttattcaatt atcgatgcct
     6301 ctaatcaatc tttcaacgtt agttacagat tataaaaagg cagtcggtgc aagtagtaga
     6361 atatatgaaa tcatgcaaga acctattgaa ccgacagaag ctcttgaaga ttctgaaaat
     6421 gtattaattg atgacggtgt gttgtcattt gaacatgtag actttaaata tgatgtgaag
     6481 aaaatattag atgatgtgtc gttccaaatc ccacaaggtc aagtgagtgc ttttgtaggt
     6541 ccttctggat ctggtaaaag tacgatattt aatctgatag aacgtatgta tgaaattgag
     6601 tcaggtgata ttaaatatgg ccttgaaagt gtctatgata tcccgttatc taagtggcga
     6661 cgtaaaattg gatatgttat gcaatcaaat tcgatgatga gtggtacaat tagagacaat
     6721 attttatacg gaattaatcg tcatgtttca gatgaagaac ttattaatta tgctaaatta
     6781 gcgaactgtc atgattttat catgcaattt gatgaaggat atgacacact tgtaggtgaa
     6841 cgaggattga aactgtctgg cggacaacgt caacgtattg atattgctag aagttttgtt
     6901 aaaaatcctg atattttgtt acttgatgaa gcaacagcta atctcgatag tgaaagtgaa
     6961 ttgaaaattc aagaagcttt agaaacattg atggaaggta gaacaacgat tgtcattgcg
     7021 catcgtttgt ctacaattaa aaaagccggt caaattatat tcttagacaa aggacaggta
     7081 acaggtaaag gtacgcattc agaactgatg gcatcacatg cgaagtataa aaactttgta
     7141 gtgtctcaaa aattaacaga ttaattttat atatataagt aagcttggag caaatacaca
     7201 tataccatcg aggaaattaa agtgtggcac attgatggat atagatgtta ataaattgct
     7261 tcaagctttt gtctatttta aatcatttga gaagttacga cataataatt cttaaattaa
     7321 tgaaatcgat attttaagaa aaaaatgctc atggtataat acaagttata agcaaacata
     7381 catatattaa atactgtagc caagagtcat aattcttcat attttacata gcaatttaac
     7441 tgattttaga gtccacggta cagaagtttg atatttcaat gtttctaaat ttttaaaaaa
     7501 ttaaatcata ggtgggtgcc aaatgttttt attaatcaac attattggtc taattgtatt
     7561 tcttggtatt gcggtattat tttcaagaga tcgcaaaaat atccaatggc aatcaattgg
     7621 gatcttagtt gttttaaacc tgtttttagc atggttcttt atttattttg attggggtca
     7681 aaaagccgta agaggagcag ccaatggtat cgcttgggta gttcagtcag cgcatgctgg
     7741 tacaggtttt gcatttgcaa gtttgacaaa tgttaaaatg atggatatgg ctgttgcagc
     7801 cttattccca attttattaa tagtgccatt atttgatatc ttaatgtact ttaatatttt
     7861 accgaaaatt attggaggta ttggttggtt actagctaaa gtaacaagac aacctaaatt
     7921 cgagtcattc tttgggatag aaatgatgtt tttaggaaat actgaagcat tagccgtatc
     7981 aagtgagcaa ctaaaacgta tgaatgaaat gcgtgtatta acaatcgcaa tgatgtcaat
     8041 gagctctgta tcgggagcta ttgtaggtgc gtatgtgcaa atggtaccag gagaactggt
     8101 attaacggca attccactaa atatcgttaa cgcgattatt gtgtcatgct tgttgaatcc
     8161 agtaagtgtt gaagagaaag aagatattat ttacagtctt aaaaacaatg aagttgaacg
     8221 tcaaccattc ttctcattcc ttggagattc tgtattagca gcaggtaaat tagtattaat
     8281 catcatcgca tttgttatta gttttgtagc gttagctgat ctatttgatc gttttatcaa
     8341 tttgatcaca ggattgatag caggatggat aggcataaaa ggtagtttcg gtttaaacca
     8401 aattttaggt gtgtttatgt atccatttgc gctattactc ggtttacctt atgatgaagc
     8461 gtggttggta gcacaacaaa tggctaagaa aattgttaca aatgaatttg ttgttatggg
     8521 tgaaatttct aaagatattg catcttatac accacaccat cgtgcggtta ttacaacatt
     8581 cttaatttca tttgcaaact tctcaacgat tggtatgatt atcggtacat tgaaaggaat
     8641 tgttgataaa aagacatcag actttgtatc taaatatgtg cctatgatgc tattatcagg
     8701 tatcctagtt tcattattaa cagcagcttt cgttggttta tttgcatggt aatatgtcga
     8761 agagtgacta tgataataca ttttaactaa taaatatgtt caggcatgtc gtctattgat
     8821 ataggtgaga tgcttggact tttttattat tgatataaag atatttaaat atttttaaag
     8881 ttaccgaaat tgaagcatta taaaaaccaa gtgtacatgg taatacactt ggcttttatg
     8941 ggaaatgaat attattgtac atatgacagt aaggactagg tacagtcata gtacttcgag
     9001 caaaatttgt tttgttatta taaacaacac aaaggagata acttctctat tgaagaagtt
     9061 aaaaacatta tagcagacaa tgaaatgaaa gtaaatta
//
LOCUS       DAHHRR010000102         8755 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.102, whole genome shotgun sequence.
ACCESSION   DAHHRR010000102 DAHHRR010000000
VERSION     DAHHRR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8755
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.102"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(19..127)
                     /locus_tag="NBI42_002072"
                     /pseudo
     CDS             complement(19..127)
                     /locus_tag="NBI42_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(227..763)
                     /gene="nrdG"
                     /locus_tag="NBI42_002073"
     CDS             complement(227..763)
                     /gene="nrdG"
                     /locus_tag="NBI42_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCD1778469.1"
                     /translation="MTLLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            complement(760..2610)
                     /gene="nrdD"
                     /locus_tag="NBI42_002074"
     CDS             complement(760..2610)
                     /gene="nrdD"
                     /locus_tag="NBI42_002074"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCD1778470.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            complement(2857..4227)
                     /locus_tag="NBI42_002075"
     CDS             complement(2857..4227)
                     /locus_tag="NBI42_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HCD1778471.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKPWIKWVNTALTLAVILAMLMNIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIALL
                     MGIVTI"
     gene            complement(4549..4644)
                     /locus_tag="NBI42_002076"
     CDS             complement(4549..4644)
                     /locus_tag="NBI42_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778472.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            complement(4720..5325)
                     /locus_tag="NBI42_002077"
     CDS             complement(4720..5325)
                     /locus_tag="NBI42_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCD1778473.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLSQYIEHIMVISPEI
                     TEKLQNMVDKGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKQALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDMNHSEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            complement(5388..7268)
                     /locus_tag="NBI42_002078"
     CDS             complement(5388..7268)
                     /locus_tag="NBI42_002078"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HCD1778474.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSESNQQQLETEVWQQLQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLYHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEED
                     AEKWMADIINMIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESERF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            7463..7555
                     /locus_tag="NBI42_002079"
     CDS             7463..7555
                     /locus_tag="NBI42_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778475.1"
                     /translation="MSVVDAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(7663..8160)
                     /locus_tag="NBI42_002080"
     CDS             complement(7663..8160)
                     /locus_tag="NBI42_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCD1778476.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEVFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(8170..8430)
                     /locus_tag="NBI42_002081"
     CDS             complement(8170..8430)
                     /locus_tag="NBI42_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778477.1"
                     /translation="MIIMRGYNERYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVIEKCITLYNVCSRCKRALILSEHSKNNIFNIKKAV"
BASE COUNT         2697 a         1690 c         1225 g         3143 t
ORIGIN      
        1 taaaacgcta tcaaaaagtt agacttcctt cccactaaaa taccactttt tctttcaact
       61 ttttaaaaaa cggatatgca atttttagtt ttggtatcaa aatgattgtt agatcatatt
      121 ctatcaatat atttttctaa agaattgctt ttattaactt tcaattatgt acctaaccta
      181 aaaagaagcc aaggcaacgg atgttacctt gacttctaat acatattcaa ctaacaatat
      241 attcaatcat acgcgcatgc gagagtgatt gttgtacatc tataatgcgt tgatttaaag
      301 aacctttata tggtaaatca ggtttgaata agtgttgtat aaatagccca tctactaaaa
      361 cgtcaatgta tgataataac tctcgacgtt ctgtacaatc atttgctaaa tattcatata
      421 aaaatccagt ccatacccaa attgtctttg tatttccaaa acgtgctcga aatgctttga
      481 caagatttaa tgtaatatcc aaattacaaa atggttcgcc acctaataga cttagcccag
      541 atatataatc atgatcgcaa tcatctaata tttctgctaa tatttcatca gtgtatttct
      601 cgccatatct gaacttttgt gaggctttgt tgtaacatcc aacacaatta aatggacatc
      661 ctgatacata aacactgcat cttactcctt caccgtcaac aaagctattt gattctattt
      721 tagcaatata accttgtcct tgtttaatgt ctaaaagtgt cattctttag gcgctttcat
      781 atgttttact cgtgcgcaaa tttctttatg acggccttta attactggac gttgaactgg
      841 attgcctaag taaccacatg ttcgtttaac gacatcaact gttttaggat tatcattgcc
      901 acagttcggg catttaaatc ctttttcagt tgcttcaaaa tctccatcgt aatcacattc
      961 ataacaatga tcaatcggaa tatttgtacc taagtaacca actttgtcat aagagtagtc
     1021 ccataccgct tctagtgctt tcaaattgtg ttgcaatttc ggatactcac aatagtgaat
     1081 gaaaccacca ctcgcataat aaggataatc tttttcaaaa tctaactttt caaaaggtgt
     1141 aacatcttta cgtacatcat aatggaaaga gttttgataa tatcctttat ctgtaatgtc
     1201 tttaatatct ccaaatctct cttggtctaa acgacaaaaa cgatccgtta gcgattcact
     1261 cggcgtactg taaatactga accaaatgtc atataattct gtccatttcg tttgataacg
     1321 tttcatttct ttaagaattt caagcgtaaa tgcttttgct tcttgagatg tttcccagtc
     1381 tggaccatag aaaacagtag ctgtttcata caaccctata tagcccattg aaatcgttgc
     1441 acgtttattt ttaaataact cagcaacatc atctgtttct tttaatttat agttaaatgc
     1501 gccactttta tataaaatcg gtgcgttatt cggtacagca tctttcaaac gatttatacg
     1561 ataaagtaat gcatcatgta acacatcgat acgttcataa aagatttccc agaatttcgt
     1621 catattaccg gcagattcta atgccattct aggtaaatta agtgtaacaa caccaagatt
     1681 acaacgacca ttattttcaa aatgaccttc cgcatctttc caacttggta aaaatgaacg
     1741 acaacccatt ggcgctttga aatcacctaa tatttctacg agtttgtcat aatttaaaat
     1801 atctggatac atacgtttcg ttgaacactt taatgctagt tgtttaatgt catagttcgg
     1861 atcttgcgga ctaaagttgg ttcctttttt aattgaaaat acaagttttg ggaaaatcgc
     1921 tgtcgtgcgg tcttttccta agcctttgat acgagtattt aagatagctt gttgaatttt
     1981 gcgacttaaa tgatctgtgc ctaagccgaa tcctaatgtt acaaaaggtg tctgtccatt
     2041 agatgtatat aaggtattaa tttcatattc taaactttca atcgcatcat tgatgtcttt
     2101 agtgacttgt tgatcaacat aacgatcaat ttcagattct ttgacaaatt gctttgcgat
     2161 atttctatgt tgttcttcat tatgtcgtgc atatgtacta agtaattcgt caacacggtc
     2221 aaccgtacag ccaccatatt gactgctaga aacattggct ataatttgta caagctgcgc
     2281 tgatgcagtt tgtattgatt ttggtgaagt tacattcgcg ttgcctattt caaatccatt
     2341 atgtagcata tttttagcat ctattaaaca acagtttgtt aacggttgga atggatgata
     2401 atctaagtcg tgaaaatgta tgtcccctct ttgatgtgca tctgcaacgt gctttggtaa
     2461 taaatgattt aatgcgtaag atttagaaac gatacctgct gttaaatctc tcattgttga
     2521 aaatgtatca ctatctttgt tggcattttc gttaacaata gcaggatctt tagaaatcaa
     2581 acccgttaat gcttcttcga tttgattcat tttacccctt cttcctatat attgtgtcta
     2641 ctcattacgt tataccacta tatatagtat tttcaaattt ttattagtct gttaactaaa
     2701 acgtcacaaa gttattaaca tttagtgaaa ttcatatttc tacacaatta tttcattaat
     2761 ttttaaaatt gtttaaagat tcgtccgtca ttccgacatg tttatgcaac tattatttca
     2821 ttttgcaacg tgaccatagt ttctattttt tcatacttaa atcgtcacaa tacccatcaa
     2881 caatgcaatt actaacataa cgatggcgaa tccccaaatc caaaagaatg catacttaat
     2941 atacgtgccc atatttgcct ctgctaaacc aattgccaac cataaggctg gtgaaaatgg
     3001 gctgacaaat gtacctataa tattccctat gaccattgaa taagctgttg atacagaggg
     3061 tacaccaaat tgccctgctg tttgttcgac gatcggtaac acagcaaaat aataagcgtc
     3121 tgtgctagtt agtaaatcta atggtacgcc aagtaaacct acaataatat gcaagtatgg
     3181 tcctacttct gcaggaatca ctttgattaa attcgtcgca atcgctttaa gcataccggt
     3241 ttcatttagt acacctaaaa acatacctgc tgcaataatc actgcagcca tcattaatgc
     3301 attcggcgca tgcgctctta atcgctccat ttgttcatcc actgatttaa aattaataac
     3361 aagtgccaac gaaacaccta tcatgaatgc aaattcaggt ggcgcaatat tcattaacat
     3421 cgctagaata acagctaaag ttaaagctgt attcacccat tttatccatg gttttgttcg
     3481 tgcacgtcct tttacaggaa accttacatc ttgatctcgt tcatatactt caactaattt
     3541 atgtacatct atatcttgtg tttgcggtaa ttcatttctc tctattgctt ttttgatacg
     3601 tttttgttct ttaaatccaa gatataccgc aaacaacata acaagaatga atcctattat
     3661 ttgaatagga attaatccat accataattc attgacactt ttggctttta aaacagcagc
     3721 tacccgcgcc atcggacctc cccaaggtac catgttcata attgcagcac ttaatgctaa
     3781 tagtaaaatc aataaatatt tattcatgtt taacgcttta tataatggta ataacgcagg
     3841 tatagaaagt aaaaatgtta ccgcacctgc accatctaat tgcgctattg tgccaattaa
     3901 agctgtcatt gcacagacaa taacgacatt gcctcgtgtc attaatatta agcgttttac
     3961 gagtggcttg aacaaaccac tatcgttcat gatgccaaag aaaataatgg caaagataaa
     4021 cataataaca acgttgatga cttggtctaa ccctttagca aaaaatccaa ccaaatctgt
     4081 cacactatat cctaaaatca ttgcccctaa gcaaggtatg attgtcatac ctacaactgg
     4141 atttatcttt ttggcaatga gtaaacctac aattgaaata ataataatga gccccattac
     4201 tgttaaccac atattatcac tattcatatt attttctccc tccactttta atacaattta
     4261 cataatcccc ctctttaact taaatattat aattaatata attttttata tttaagttat
     4321 ctcattttat caaaaaaatc ttttttgcgt aactatcaat agtgaagttt gattttccta
     4381 cttgataaca tggcatgact aatccttgta tatcctgtcc tgtatttcaa tttgatttat
     4441 catttttatt attgctttct attgcacaaa aaatctggct aaaggtcgct atgatagtaa
     4501 cgacttcaac cagatttcaa tttatccatc tattttatta agtttgttct acgtatcttt
     4561 ttctctattg taatattcat atttaaaacg catatcatta tcgcttaaat ccttcattat
     4621 tagactattc acaaagccgc ccatgtctta atttcaatga ttaagcgtta aagttaaaat
     4681 aagaagactt accttagacg gttagacggt ccgctattat tatcttacat ccaaccacgc
     4741 taaaaattga gcttgtttgt catgatttaa atattcttgt gacacaattt gtgacagtaa
     4801 ctgttgcttt tcgctatgat tcatatcaag tacttttatt ttctgccggc aagtatataa
     4861 aaagtcgata tacgaactgt atgatggtgg atataacgcc tcaagctctg ccataattga
     4921 ttttgtcaac ttaggactcg caccatcagt tgatacactt atagttaact tgtcgcggtg
     4981 tagtgcactt ggaaatacaa cattgccatt tgatgcatct ccaacattat taaaaagggc
     5041 atgctcaggt aaggcttgtt ttaccgcttc attgacacgt ggctcattgg ttgctgcaat
     5101 aactagatac gcgtctacaa tatcgcttgg ttcaaactct ttttctttcc atatgacgac
     5161 acctttatct accatatttt gaagtttttc agtgatttca ggactgataa ccattatatg
     5221 ttcaatatat tgacttaatg tttgtgcccg acgacttgca acgacgcctc caccaactat
     5281 gacgacattt ttatttgtta aatctatcat taatggcata ttcatgttga gcaacatcct
     5341 ttatataatt ttacatcatt acaccttaaa atatttaaca ccaatcgcta ataaacatca
     5401 cgttgataac gttgttgctg tttcatttgt cgcaataata actctgcttc ttcttgagaa
     5461 atatgacgtt cttttaccaa tacatcttta atggcttgat ggacatcctt cgccatacat
     5521 ttttcatcgc cacaaatata gattgctgcg ccttgctcaa tccattcatt gaaacgttca
     5581 ctttcttcca ttatacgatg ttgtacatat tctttatgtt cttggtctct tgaaaatgct
     5641 aaatctacgc gtgttaagtt tccattttca agccattctt ctatttcttc ttcatataaa
     5701 aagtcagaac tacggtgttg atcaccaaag aacaaccatg tttttccagt cataccaagt
     5761 tcttcacgtt cttgtaaata tgctctaaaa ggagcaattc cagtacctgg tccaatcata
     5821 ataaccggta tatcttgctt catcggaaat ttgaagttcg gatttttctt caaataaatt
     5881 ggtactatat cgcctggttt aattcgctca gcaaaatgaa ccgagcatac accttttctc
     5941 tcacgtccat gtgcttgata acgaaccgta ccaacggtaa tatgcacttc atctggcgtt
     6001 gccataaaac tactagaaat cgaatactct cttggtggta attttctaag gatttgatac
     6061 atattctcag gttgtaattc tatagttgga aaatctgtta ttaaatctat aaagtcccga
     6121 tttataacat attcacgcgc ccatgactca tcttgaatac gttcagataa ttcttcattg
     6181 tcaaaataga tatctgcatt tttcaataat ggcaatgtta atttagtaaa ttcaaaatgt
     6241 gatgttagtg cttcaacaat aggaactgta tcaccatgat cattaattgg caccggagat
     6301 tgcggatccc aacctaacat ggatattagt ttttcaacca attcagggtc gttttgcggt
     6361 aatgctacta tacaatctcc tggttcatat gattcactaa aatcatcaag taaaaattct
     6421 atatgtcgtg tttctttatt tgaatcggta ccatttaaat tgatattcgc taaaacttct
     6481 gcttggtatg gatttgattt agaatatttc ttttctttgg cagacttaat tgattcactt
     6541 atcaccgatt cactttgaat accttctgat gtggtatcaa tcatattaat gatgtctgcc
     6601 atccactttt ctgcgtcttc ttcataatca atatcacaat ctacacgctt acatatacgc
     6661 tcagcgccta gattttctag taaagcatct acatctttac ctgcttgaca gaaaaattca
     6721 tatgtttgat cacctaaagc tagtactgaa tatctcacat gatataaatt aggtgcgtta
     6781 tcgtcttcta aaaattcaaa gaaatcccat gcattatcag gcggttctcc ttcaccatga
     6841 gtagacgtaa taataaataa atcttctaac tgtgcgatgt ttgtcgtatc atattcatcc
     6901 attgacatca aaacaacttg atgtccgata tcacttaaac gttctgaaaa aatttcagct
     6961 aatcgcatgg cattacctga ttcagaacca tatagcactg tgacatgccg ctgattagct
     7021 tcgatatgtg gctctttatt ttgtaacata tacgttgttg aagttgcttg ttcttctgat
     7081 atttgtaatt gttgccacac ttctgtctct aattgttgtt gattactttc acttgtcgta
     7141 tcgttactat tagctagtaa aaatccacta agccactgtt gttggctctc tgttaatgtt
     7201 tgcagcagat tgttaatctc tgtgacttgt ttttctgtaa atggactgtt agatgtattt
     7261 aatttcaaat gttttcacct caataaactt gaaatagatg attgactcat tttttatcga
     7321 tagtttagtc aattttttaa tatgtatggt ataaattaaa acaaacttga gttgtgtctg
     7381 atagttatac ttgcaaatat ttacaatctt catgttgctt gaatgacatt atcagtaatt
     7441 cagacaaaac tttgattatg tcgtgtcagt tgttgatgca tacagtaatg catcgttgtt
     7501 attttttatt tatttactat ttattatgta ccaaatcagc ctttcagtaa aataggtcaa
     7561 attactgatt ttctaaatat aaaatgcctc ctaatgacat actattagta catcattaag
     7621 aggctcttgt gttatttgca tactaagcgc tcaatttcaa atttaagatg aagattcttg
     7681 caataatatt tctatatttg tcgatatatc caatggatct tccattggca agaatcgatt
     7741 tacaacattg ccttgtcgat cgattataaa ttttgtgaaa ttccatttta ttggggaccc
     7801 aaagattcct ggttgttcat tctttaaatg cgtaaataac ggatgttcat cgttcccgtt
     7861 cacagatatt ttagctagca ctggaaatgt aacaccaaat ttctcacgac taattttcaa
     7921 gatttcttca ttagaacctg gttgtcgatt gtcaaaatta ttattcggaa aactcaacac
     7981 tacaaaccca cgatccttat atttttgaaa caaagtctct agttttttca attgttcgct
     8041 atatatacat tccgttgcag tattaacaac taaaatcact ttacctctaa atacttctaa
     8101 tttataagtt aagcctttat aatcacttac ttcgatatca tacacatttc tattattcat
     8161 aagacacccc tacacagcct tttttatatt gaatatgtta tttttagaat gttctgataa
     8221 aataagtgcg cgtttacacc gcgaacacac attatatagc gtgatacatt tttcgatcac
     8281 acgataaata atgttcgagt ttatgttgtt gctcaaccta tccgatttac cgtctttttt
     8341 cacctgtctt actttattaa aataatcaca ttgtatagct gtattagtcc agctacaata
     8401 tctttcgtta taacccctca ttataatcat ccttattttc tatttttaaa aagacaatta
     8461 gaccgctctt taaactatag attaatactt aagttaaact catacatact gataccatac
     8521 gttaaatcta acaatttaaa attcgttata actatggatt aaagagctgc ccaactcata
     8581 taatccttaa aaacttcaca tgtgattgtt tattaagccc tcctttatct tattaaatat
     8641 ccttataacc cttttaaaat taaactgaca cactcataca ttgttacacg cgacgataaa
     8701 cgtcatatta ttgcatgtca ttaaaacaag tcactttgta tcgtgtgcga ccgaa
//
LOCUS       DAHHRR010000103         8612 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.103, whole genome shotgun sequence.
ACCESSION   DAHHRR010000103 DAHHRR010000000
VERSION     DAHHRR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.103"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..230
                     /locus_tag="NBI42_002082"
     CDS             <1..230
                     /locus_tag="NBI42_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1778478.1"
                     /translation="LEDYSLSEIADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKF
                     EQRREIYDEMKQHLSNPEQIQRYIQQLEDLE"
     gene            256..1623
                     /gene="ffh"
                     /locus_tag="NBI42_002083"
     CDS             256..1623
                     /gene="ffh"
                     /locus_tag="NBI42_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCD1778479.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTKLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            1965..2240
                     /gene="rpsP"
                     /locus_tag="NBI42_002084"
     CDS             1965..2240
                     /gene="rpsP"
                     /locus_tag="NBI42_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCD1778480.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            2427..2930
                     /gene="rimM"
                     /locus_tag="NBI42_002085"
     CDS             2427..2930
                     /gene="rimM"
                     /locus_tag="NBI42_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCD1778481.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEYYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIK
                     PMEGLLD"
     gene            2930..3667
                     /gene="trmD"
                     /locus_tag="NBI42_002086"
     CDS             2930..3667
                     /gene="trmD"
                     /locus_tag="NBI42_002086"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCD1778482.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            3770..4120
                     /gene="rplS"
                     /locus_tag="NBI42_002087"
     CDS             3770..4120
                     /gene="rplS"
                     /locus_tag="NBI42_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCD1778483.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(4338..5656)
                     /locus_tag="NBI42_002088"
                     /pseudo
     CDS             complement(4338..5656)
                     /locus_tag="NBI42_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5879..8485)
                     /gene="yfhO"
                     /locus_tag="NBI42_002089"
     CDS             complement(5879..8485)
                     /gene="yfhO"
                     /locus_tag="NBI42_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HCD1778484.1"
                     /translation="MLKKWLNSNVKQFLVITFMSVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLSSFSSLYDASFGLGGDYMKGLSYYYSLSPIMWLNFLFIK
                     IGETIGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSTNMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFFITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVSI
                     IALLYVLLSPTHPLALIVGIILLMVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGPFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNINDRIRV
                     NHDDNLPYGFKINSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNTKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRFVTPVTIRIKASDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKQGYTITKNKNSSGYIVLP
                     TAYNQGMEATSGDQSLKVEKVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITVFGII
                     CSIIFTRWARQK"
BASE COUNT         3032 a         1250 c         1501 g         2829 t
ORIGIN      
        1 atctcgaaga ttattcttta agtgaaatcg cagatacttt taatgtgagt agacaagcag
       61 tttatgataa tataagaaga actggcgatt tagttgaaga ttatgaaaag aaattggaat
      121 tataccagaa atttgagcaa cgccgagaaa tatatgatga aatgaaacaa catttaagta
      181 atccagaaca aatacaacgt tatattcaac aattagaaga cttagaatag atataaattg
      241 agggaggaca tcgatatggc atttgaaggc ttatcagaac gcctgcaagc gacgatgcaa
      301 aaaatgcgtg gtaagggtaa acttactgaa gctgatataa agataatgat gcgtgaagta
      361 agattagcgt tacttgaggc tgacgtaaac tttaaagtgg taaaagaatt tattaaaaca
      421 gtatcagaac gcgcattagg ttccgatgta atgcaatcat taacaccagg gcaacaagtt
      481 attaaaatag ttcaagatga attaacgaag ttgatgggtg gagaaaatac atcgattaat
      541 atgtcaaata aaccacctac tgttgttatg atggttggtt tacaaggtgc tggtaaaaca
      601 acaactgcag gtaaattagc attattgatg cgtaaaaaat acaacaaaaa acctatgtta
      661 gttgcagcag atatttatcg tccagcagcg ataaatcaat tacaaacagt agggaaacaa
      721 attgatattc ctgtatacag tgaaggagat caagtaaagc cacaacaaat tgtaactaat
      781 gcattaaaac atgctaaaga agaacattta gactttgtaa tcattgatac agcaggtcga
      841 ttacacatcg atgaagcatt gatgaacgaa ttaaaagaag taaaagacat tgctaaacca
      901 aacgaaatta tgttagttgt cgattcaatg acgggtcaag atgctgtcaa tgttgcagaa
      961 tcttttgacg atcaacttga tgtcacaggt gttaccttaa ctaaattaga tggtgataca
     1021 cgtggtggtg cagctttatc tattcgttcg gtgacacaaa aaccaattaa atttgttggt
     1081 atgagtgaaa agttagatgg tttagagcta ttccatcctg aacgtatggc atcacgtatt
     1141 ttaggtatgg gtgatgtgtt aagtttaatt gaaaaagcgc aacaagatgt ggatcaagaa
     1201 aaagcaaaag atttagagaa aaagatgcgt gagtcatcat ttactttaga tgatttttta
     1261 gaacaacttg atcaggtgaa aaatttagga ccactggatg atattatgaa aatgattcca
     1321 ggtatgaata aaatgaaagg tctagataag cttaatatga gcgaaaagca aattgatcat
     1381 attaaagcga ttatccagtc aatgacgccg gctgaaagaa acaatccaga cacattgaat
     1441 gtatcacgta aaaagcgtat tgctaaaggt tctggtcgtt cattacaaga agtcaatcgt
     1501 ttgatgaaac aatttaacga tatgaagaaa atgatgaaac aattcactgg tggcggtaaa
     1561 ggtaaaaaag gtaaacgcaa tcaaatgcaa aatatgttaa aaggtatgaa tttaccgttt
     1621 taatgtttaa tataaacacc aatctttatt tcaacttaag gttggtgttt ttgcattaat
     1681 aatattgtaa ttaaagttat ttagagggac atgtacagtt gtagtagtac cgttcatgtg
     1741 aatataatgt ttaattatat cattttgtat aacttggtag taaatgcatt caggaaaagt
     1801 attaagcgtt tcatcgagct aatttaattt ttctagattt tgggataaaa tattcaataa
     1861 atttctaggt aaagaaaaaa ctctttacaa acattcatac acctgttaat attatttctt
     1921 gtagaaaata aaaattaaaa catgacttaa aggagatttt ataaatggca gttaaaattc
     1981 gtttaacacg tttaggttca aaaagaaatc cattctatcg tatcgtagta gcagatgctc
     2041 gttctccacg tgacggacgt atcatcgaac aaatcggtac ttataaccca acgagcgcta
     2101 atgctccaga aattaaagtt gacgaagcgt tagctttaaa atggttaaat gatggtgcga
     2161 aaccaactga tacagttcac aatatcttat caaaagaagg tattatgaaa aaatttgacg
     2221 aacaaaagaa agctaagtaa tttagcgtaa aattgttcta acaataagaa taactcgttt
     2281 acactgacag ttattactca atgatacgtt gggaatatca catttagtaa tatagaacgt
     2341 attgggtacc ataatggtgc cctttttctt tgaattattt tcaattaaaa tagaagtgtt
     2401 caaagcatag agttggaggt aatagaatga gagttgaagt tggtcaaatt gttaacacac
     2461 atggtattaa aggtgaaatt aaagtaaaat ccaattcaga ctttacagac gttcgttttc
     2521 aacccggtca agtgctgaca gttgtgcata acaataacga ccttgaatat actgttaagt
     2581 cacatagagt gcataaaggg cttcatatgc tcacatttga aggtattaat aatattaatg
     2641 atattgagca tttaaaaggg agttctattt atcaagagcg tgatcatgaa gatatcgtac
     2701 ttgaggaaaa tgaatattat tattcagata ttataggatg tacagttttt gatgatcaag
     2761 aaacaccaat aggtcgtgta attaatatat ttgaaacagg tgcgaatgat gtgtgggtga
     2821 ttaaaggatc taaagaatat ttgattcctt atattgctga tgttgtaaaa gaagtggatg
     2881 ttgaaaataa aaaaattatc atcaagccaa tggaaggatt gttggattaa tgaaaattga
     2941 ttatttaact ttatttcctg aaatgtttga tggtgtttta aatcattcaa taatgaaacg
     3001 tgcccaagaa aacaataaat tacaaatcaa tacggttaat tttagagatt atgcaattaa
     3061 caagcacaac caagtagatg attatccgta tggtggcgga caaggtatgg tgttaaagcc
     3121 tgaacctgtt tttaatgcga tggaagactt agatgtcaca gaacaaacac gcgttatttt
     3181 aatgtgtcca caaggcgagc cattttcaca tcagaaagct gttgaattaa gcaaggctga
     3241 ccacatcgtt ttcatatgcg gacattatga aggttacgat gaacgtatcc gaacacatct
     3301 tgtcacagat gaaatatcaa tgggtgacta tgttttaaca ggtggagaat tgccagcgat
     3361 gaccatgact gatgctattg ttagactgat tccaggtgtt ttaggtaatg aacagtcaca
     3421 tcaagacgat tcattttcag atgggttatt agagtttccg caatatacac gtccgcgtga
     3481 atttaagggt ctaacagttc cagatgtttt attgtctgga aatcatgcca atattgatgc
     3541 atggagacat gagcaaaagt tgatccgaac atataataaa agacctgact taattgaaaa
     3601 atatccatta actaatgcag ataagcaaat attagaaaga tataaaatag gattgaaaaa
     3661 aggttagtat tgtgctaaca taatctgagt gtggaaacac aaaaatcact atgatccgct
     3721 gctatatatt tgtcgaggca agaacatagg ttgaagagga gaatttataa tgacaaatca
     3781 caaattaatc gaagcagtaa ctaaatcaca attacgtaca gacttaccaa gtttccgtcc
     3841 tggtgatact ttacgtgtac acgtacgtat cattgagggt actcgtgagc gtatccaagt
     3901 attcgaaggc gttgtaatta aacgtcgtgg cggtggcgtt tctgaaacgt ttacagttcg
     3961 taaaatttca tcaggtgttg gcgtggaacg tacattccca ttacacacac caaaaattga
     4021 aaaaatcgaa gttaaacgtc gtggtaaagt acgtcgtgct aaattatatt acttacgtag
     4081 tttacgtggt aaagctgcta gaatccaaga aattcgttaa tcagcattta aacaaagcta
     4141 tgtatgagtc atattcgact caaacaacaa agccatctag gtcactttta tggagtgata
     4201 tagatggctt tgttgttttg ttaatgtaaa gttgataatg taattgtttt tcccttcttt
     4261 attcggttct ccaccaaatg tggtgggtat ataatttaaa gaattatttt aaattacaac
     4321 ttttagagtt tttattatta ggcggccagt ccattattgg gcttggttgt cttctttttt
     4381 tctcctttgt acaagctgaa aatcatcatt atacgtgctt taaagttgtt gaaatttcta
     4441 taaccaaatg aaattcgctt gattaatttt atcttattat taattccctc tatagcacca
     4501 ttattaaatg ctggataata aactgtattt cttaacatcc tttggtgctt tctataatat
     4561 ttaaccactt tccatacacc tttactcaca gactttctac taactgaatt taaacgatta
     4621 ataaatttag gccaattaca taactttagg tcttcccgta atccttgaac aagttcgtag
     4681 gagtgtcgta gtatatcgtc ttttgaaagc atgaactcta caatgtcaga tgagcgttta
     4741 taagccttga aagacttatt ccatctatat ttactaaata tggttttact agtatccatc
     4801 aatagaactt tccagttatt cttgaaaatt gtataatcag gacctttttt attgcgatat
     4861 tcattcataa cttgtacacg atacttatta agttctctat ttaaatgttg aacgatatgg
     4921 aatctgtcaa aaataatagc tgcattagga aataggtcgc gaaataatcg gacatagggt
     4981 tcatacatgt caatagtaac cgtcttaact tgttgtcgat tttttaaatg gaatcgctcg
     5041 aaataggcac gcaagaatct tgtagttcta ttttctaaaa tatctataac atcatgagta
     5101 tcattatcta taaaaatgaa actcattgat ccagttacat ttttaacact tttaaattca
     5161 tccatagcga tgtgttctgg caaacaatta aacggtttaa ttcgtaatga attagccgct
     5221 ttagttctaa tacgacttac agtagatgga gagatacagt tatcttctgc agtatcaatc
     5281 tctgttttaa ctttagtagc ttcttctata actttttgag taacacatct tgagataaaa
     5341 cacgactcat caacaattga tgttttagca gtaaaagtgc cattgcattc taagcattta
     5401 aaacgttgtt tagctaagtt gaggtatgtg ttataccctt gagttttaag caaagtaata
     5461 cgagaaacac gcttaccatg tttatgaatt tgtccttcat ttttaactcc acaacagtaa
     5521 caggccgaag gcttatacgt tagtacagca ttaaccactg ttgctttttt cctttgataa
     5581 tgacgtcaat ttcttggttt atatatttaa tattttcatc ttttattctt agtaattcta
     5641 aggtatcatt acacataggc gcacttgtct cctttaattt aggtttggtc actttaaatt
     5701 atagaggcaa ttgcgctttt tgtgtattaa aaaaagcagg aagttttacc ttcccaccgt
     5761 aaaagatgaa gaacccttta ttcataacgt gtttgctcaa aatggttata taaacaaaaa
     5821 tctactaagt gccattgaaa aggacttagt agatttatta aatatatgcg tagcaacatt
     5881 atttttgtct tgcccatctc gtgaaaataa tactacatat aatgccaaat acagtaattg
     5941 ttataagtaa ataatagtat ggtggggtat agctcaattg aatctttgtt atatttttag
     6001 gtgctttaat gccggtcata acaccattta ctttttcaac tttaagactt tgatcacctg
     6061 atgtcgcttc cataccttga ttatatgctg ttggcaaaac aatataccca gatgaatttt
     6121 tatttttagt aatagtataa ccttgctttg tcttactaac tttgacagcc tctaatgaat
     6181 ttgaagcgtc tttaagcgtg gtataatctt cgccgtatat cccttttaaa tttacacgat
     6241 acttaccttt aggcaatgat aatctaatcc tatctgaagc tttaatgcgt attgttacgg
     6301 gtgttacaaa gcgtcgatat ttataagtga gtttatttct ttcttgtgta tattcattca
     6361 ctttaacatc atgagcttta tccggcgaaa gtaattctaa atccatttca aaatacaaat
     6421 ctttaaattg attagaaact gattttggca actgtacagt tagaccacca ttattttgtt
     6481 taacttgtaa taaatgtttt gtaggagatt gccaggctgc actatttaat ttaattgttg
     6541 aatctgatag taaattttta ttggctttaa aatgtgtatt aacatcttta gtattgttag
     6601 aaacaatccc ttgcaacatt gcttgttctt tatctaatgg agattttaat tctttattgg
     6661 aaaagacctt atttgtaata tgtgcacttg gataatggat ggtattttta gaatgaatcc
     6721 aacgaacttt attgtctttg tgttcagagt taattttaaa tccatatggt aagttgtcat
     6781 catgattcac tctaattcga tcattaatat tccaaagtga tagtaaattt tgacgattgc
     6841 caagtagtct ataagtgctg tttttatcaa ttggcatatt aatttggagt gtcttgtcat
     6901 aatattttaa aatgtctcca ttaaaaatac tagaatataa tgaaatgcca ttataatgat
     6961 atataaatgg tgaatttaat gcatagtctg acatataatc aatgcgatta aatgggcctg
     7021 ttgcattttg atttatcttt tttataagct ggtttacata gttactatgg taatcatgtt
     7081 gtttcaacgt tgataatgat tgttgataag gtttgattgc catatttttg ttgttatcta
     7141 aaatgacgat ttgttgaatc ataacgatta atactaatat tgcaacggtt aattttttat
     7201 aacgccataa actaaatttt aaaataacgg caagtaccat tagcaggata atacctacta
     7261 taagtgcgag tgggtgtgtc ggtgatagta atacataaag taatgcgatg atacttactg
     7321 gtattgttct gattaaataa tatttcatat ttaatgttga taaatgttga ataaacaatc
     7381 cgcaaagagc acttgatgat agtgctaaga tatacaccca acgcctttct ggaaatgaaa
     7441 aaccattaaa agcactgtcg aaatactgtg ataatgaacc aataaataat atccatgtta
     7501 ctattgcgaa aagtctataa aagtaaaaac gatacagttt gaatgacaat aatgcaacga
     7561 tagtaagaat tgaaatcgta atgaaaaatc catcgctaaa gaaaaaataa tgataatcaa
     7621 gtggtgtcaa aaacggtata tcaacattgg gattttgctt tctgtcattt tccaaaaacg
     7681 cagaaatgcc agtgaataaa ccaaatacac ttgataacac actcaaaact gtagcagata
     7741 tgacgcaaat taatttttgt gttctagaga caatgtcata tttgtatgtg aaaatgagtc
     7801 gatataaata gtagcaacct ataataatag cttgataata actgaaataa aaattgctga
     7861 atagtgttag ggctatcgca acaatgaaaa taccgatttt gcgttgttga aaatatcttt
     7921 ccaaaccaag aatcgataat ggcaataaat ataataaatt tccataaaat gaccaagtaa
     7981 aattaaagta tataacgaca gttgacatgc cgtataaaat cgtagcgatc atatttgttg
     8041 agcgtttaaa gtgtaatatt ttaaataagt agaaggtcac gacaaacgtt atgatagctc
     8101 gtatcatagc cataataagt tggtttgtcg gccaaaaatg tattgttgtc ggattaaata
     8161 taccaatcgt ttctcctatt ttaataaata gaaaatttag ccacattata ggtgacagcg
     8221 aataataata tgatagtcct ttcatataat cgccacctaa tccaaacgat gcatcatata
     8281 aactagaaaa actacttaga tgttcataca aatacatttg aaatggcatc atttgacgga
     8341 atccatctcc agctccacta aaaacagtac cattcacaat ataatcatag atatgagtag
     8401 aaaataaaat aagcgttaat attacgctca tgaaagttat gaccaagaat tgtttgacgt
     8461 ttgaatttaa ccattttttt aacacaacat tatcctcaac tttcaaattt aaaattaagt
     8521 ttaactgaaa ctaaagttaa tgaggttctc gataggtaaa gacaaagatg actgtggaac
     8581 agatacctta tcatagttac ttaaactttg ga
//
LOCUS       DAHHRR010000104         8204 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.104, whole genome shotgun sequence.
ACCESSION   DAHHRR010000104 DAHHRR010000000
VERSION     DAHHRR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.104"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..760
                     /gene="gcvT"
                     /locus_tag="NBI42_002090"
     CDS             <1..760
                     /gene="gcvT"
                     /locus_tag="NBI42_002090"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCD1778485.1"
                     /translation="LLVVNAANTEKDFNWILKHKEKFDVEVQNVSNRYGQLAIQGPKA
                     RDLINQLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWD
                     GLLEYKVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKS
                     VLKDQKENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALA
                     MIKRDEFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            780..2126
                     /gene="gcvPA"
                     /locus_tag="NBI42_002091"
     CDS             780..2126
                     /gene="gcvPA"
                     /locus_tag="NBI42_002091"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCD1778486.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIIV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLGGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            2119..3591
                     /gene="gcvPB"
                     /locus_tag="NBI42_002092"
     CDS             2119..3591
                     /gene="gcvPB"
                     /locus_tag="NBI42_002092"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCD1778487.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPISDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSEHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(3954..4340)
                     /locus_tag="NBI42_002093"
     CDS             complement(3954..4340)
                     /locus_tag="NBI42_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD1778488.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            4498..5328
                     /locus_tag="NBI42_002094"
     CDS             4498..5328
                     /locus_tag="NBI42_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCD1778489.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIELKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(5392..5610)
                     /locus_tag="NBI42_002095"
     CDS             complement(5392..5610)
                     /locus_tag="NBI42_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778490.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLVSFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(5624..6205)
                     /locus_tag="NBI42_002096"
     CDS             complement(5624..6205)
                     /locus_tag="NBI42_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778491.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            6310..7371
                     /locus_tag="NBI42_002097"
     CDS             6310..7371
                     /locus_tag="NBI42_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCD1778492.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQASNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRNVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGVLPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQINNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            7397..7954
                     /gene="efp"
                     /locus_tag="NBI42_002098"
     CDS             7397..7954
                     /gene="efp"
                     /locus_tag="NBI42_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCD1778493.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
BASE COUNT         2914 a         1128 c         1502 g         2660 t
ORIGIN      
        1 tttattagtc gttaatgctg ctaatactga aaaagatttt aattggattt taaaacacaa
       61 agagaaattt gatgttgaag tacaaaatgt atcaaaccga tatggtcaat tagcaataca
      121 aggaccaaaa gctagagatt taattaatca attagttgat gaagatgtaa ctgaaatgaa
      181 aatgtttgaa tttaaacagg gcgtcaaatt atttggagca aacgtcattt tatctcagtc
      241 aggctacaca ggtgaagatg ggtttgaaat ttattgtaat atcgatgata ctgaaaaaat
      301 ttgggatggt ttattagagt ataaagttat gccatgtggg ttaggtgctc gtgatacatt
      361 aagattagag gctggattgc cattacatgg acaagattta actgaatcaa ttacaccata
      421 tgaaggtggt atcgcttttg caagtaaacc attaattgat gctgatttta ttggtaaatc
      481 tgtattaaaa gatcaaaaag aaaatggtgc accaagaaga acagtgggat tagaattact
      541 tgaaaaagga attgcaagaa ctggttatga agttatggat ttagatggaa atattattgg
      601 agaagtaact tcaggaacac agtctccatc atcaggaaaa tcaattgcac ttgcaatgat
      661 aaaaagagat gagtttgaaa tgggtagaga attgcttgtt caagttcgta agcgtcaatt
      721 aaaagcgaaa attgttaaga aaaatcaaat tgataaataa ttaaaaaggg gtgtgcattg
      781 tgagtcatcg ttatatacct ttaactgaaa aagacaagca agaaatgttg caaacaattg
      841 gtgcaaaatc tataggagaa ttattcggtg atgtaccaag tgatatttta ttaaatagag
      901 atttaaatat tgctgaaggc gaagcggaaa caacgttact tagaagatta aatcgcattg
      961 caagcaagaa tatcactaaa gaaacgcata catcattttt gggtgctggt gtttatgatc
     1021 attacgcgcc atcagttgtg gatgctatga tttcaagatc agaattttac acagcatata
     1081 cgccatatca accggaaatt tctcaaggtg aattacaagc catttttgag ttccaaactt
     1141 taatttgtga attaacagat atggatgtag caaattcttc tatgtatgat gggatgacta
     1201 gctttgctga agcatgtatt ttagcattta gtcaaacaaa gaaaaataaa attatagtct
     1261 caaaaggttt gcattatcaa gcattacaag tacttcatac ttatgctaag acgcgtaagg
     1321 aattcgaagt agtggaaatc gatttagatg gcactgttac agatttgaaa aaattagaag
     1381 cagcagtgga tgatgaaaca gcagctgttg cagtacaata tccaaacttc tacggttcaa
     1441 ttgaagatct tgaaaagatt catagcttta ttgaagataa aaaagcgtta tttatcgtat
     1501 atgcaaatcc attagcattg ggattactta cacctccagg ttcatttggt gcagatattg
     1561 ttgtaggtga tactcagcca ttcggaattc ctgctcaatt cggtgggcca cattgtggat
     1621 attttgcaac gacgaaaaag ttaatgcgta aagtgccagg tagattagtc ggtcaaacac
     1681 aagatgatga aggtaatcgt ggctttgtat taacattaca agcacgtgaa caacatattc
     1741 gacgcgataa agcgacatct aatatttgtt ctaaccaagc attaaatgcc ctagcgtcat
     1801 caattgcaat gtcagcactt ggtaaacaag gtatctatga tattgcagtt caaaatatag
     1861 aacatgctaa ctatgctaaa caacaattta ttaaaaaagg ttttgaagta ttaggtggta
     1921 catctttcaa tgagtttgta gtcaaatttg acaaaccaat tcaacaagtt aacgaggaat
     1981 tagtgaagta caatattatc ggtggttttg atttaggtgt tgtttcagac gactttaaaa
     2041 atcacatgtt aatagctgta actgaattaa gaacaaaaga tgaaatcgat acatttgtag
     2101 aaaaggctgg tgagttaaat gactagtaaa tcaagtccat taatttttga aagatctcgt
     2161 gaaggcagat atgcatattc attaccaata agtgatatta aaacaaattc tgttgagtca
     2221 ttgttagatg ataaatttat tcgtaaaaac aaagcggagt ttcctgaagt tgctgaacta
     2281 gacttggtac gtcattatac tgaactttca aataaaaatt tcggtgtaga taacggattt
     2341 tatccattgg gttcatgtac gatgaagtat aaccctaaaa ttaatgaaaa ggttgctaga
     2401 ataccaggat tcagtgaatc acatccatta caagatgaag accaagttca aggttcatta
     2461 gaaattattt atagtttgca agaagaatta aaagaaatta ctggtatgga tgaggtaaca
     2521 ttacaaccag ctgctggtgc acatggtgaa tggactgcat tgatgatatt taaagcttat
     2581 catgagaata atggtgaagg tcatcgtgat gaagtcattg tgccagattc tgcgcatggt
     2641 acgaatccag cctcagcttc atttgcagga tttaaatcag ttactgtaaa atcaaacgaa
     2701 cgtggcgaag ttgatattga tgacttgaaa cgtgttgtaa atgaaaatac agcagctatt
     2761 atgttaacca atccaaacac tttaggtatt ttcgaaaaaa acattatgga aatccgtgaa
     2821 atcgtccata atgctggtgg tctattatat tatgatggtg cgaatttaaa cgctattatg
     2881 gacaaagttc gaccaggaga tatgggattt gatgctgttc atttaaactt gcataaaaca
     2941 tttactggtc cacatggtgg cggcggtcct ggttcaggtc cagtcggtgt agtaaaagaa
     3001 ctagcaagtt acttaccaaa gccaatggtt attaaagatg gcgacaaatt taaatatgat
     3061 aatgacatta aaaattctat cggacgtgta aaaccatttt atggtaactt tggtatttac
     3121 ttaagagctt atacgtatat tcgaactatg ggagcaactg gacttaaaga ggtttctgaa
     3181 gcagcggttc ttaatgcgaa ttatattaaa gcacgtttat ctgaacactt tgaaatacct
     3241 tataaacaat attgtaaaca tgagtttgtg ttaagtggtg tgcgtcaaaa agaatttggt
     3301 gtacgtactt tagacatggc taagcgatta ttagatttcg gtgtacatcc accaacaatc
     3361 tacttcccat taaatgttga agaaggtatg atgattgaac caactgagac agagtctaaa
     3421 gaaacgcttg attattttat cgatacatta attagtattg ctgaagaagc taaaaatgat
     3481 cctgataaag tgctagaagc acctcataca actgtgattg atcgattaga cgaagctaca
     3541 gctgctcgta aaccaatatt gaaatttgaa aatcttaaac aggaaaaata aggtattaaa
     3601 cacatattcc gagaattatt attctaactt tggatgaaga tttaaggata atggtttcaa
     3661 aatcaattaa aaaagacaat ttctatttga aaaagaaaac taaaccgaag taataactct
     3721 tagggtttgg tattattctt tcatagaaat tgtcttttca cttttagatt gcggtaattg
     3781 aatcgtattg aaaatgaatt gaactttctt tattatgctg aaactaagtt taatgatctg
     3841 tgaagctaga gaaatatagt tacagacttt gttaaaaaat taaaatctga gtattgttat
     3901 tgatgatcaa ttaacactca gattttatta aatcgtatat taaatttaca aaactatttt
     3961 ttagacttta tttttccagt ccatttttta tagccgcctt ttaacatata gatatctgta
     4021 tatccattct ttttcaaaat acgagcggct ctatagctag caatcccatt ggcatcacat
     4081 aagtataccg gttggtcttt tcttaatcct tggaatcgtt gcctgaacat tgtcatagga
     4141 atattgcgag acccattaat gtgaccgtag tcatagtcaa ctttctctct aacatcgatg
     4201 acttgagctt ttctaatccc attatggaat tcattttgat ctaattcttt aacagctcgc
     4261 ttgttaagaa tttgttgaac aatcatataa gcaataattg ctataactaa aattattgcg
     4321 atgtacaaac tagcactcat cttgcatcct ccctaattaa acgataatat aattataaga
     4381 ctgttaggtc aaattatcaa aatatttcac taaagaattg caattattat tcatttagtt
     4441 aaattgatta attagcgaat caaatattat acattttgct gataggagtt cttgaatatg
     4501 actgaaactt ggaattttat taatacagga agtaaagacc catattataa tatggcaatg
     4561 gatgaagcgt tattaaattt tgtctctaga ggtgaaattg atccagttat acgtttttac
     4621 acatggaatc ctgcaacgtt atcaatagga tatttccaaa ggctacaaaa agaaattgat
     4681 attgataagg ttaaagagaa aggttttggt ctcgtaaggc gacaaactgg tggccgtggg
     4741 gtgcttcatg ataaagagct aacttacagt gtgattgtac ctgaatcgca tcctaatatg
     4801 ccttctactg ttacagaagc gtacagagtg atttcacaag gattattaga aggatttaaa
     4861 aacttaggat ttgatactta ttttgcggta cctaaaacgc ctgaagagcg tcaaaaatta
     4921 aaacaacctc gaagttcagt gtgttttgat gcaccaagtt ggtatgagct agttgttgaa
     4981 ggccgtaaga ttgctgggag tgctcaaacg agacaaaagg gtgtaatcct ccaacacggt
     5041 tcgattttac aagatattga tattgatgaa ttgtttgata tgtttattta taaaaatgaa
     5101 agattaaaat taaaaatgaa agaggcattc gttgaaaaag cagttgccat caatgatatt
     5161 tcagatgaac acattactat tagtcaaatg gaagaagctt tcgagaaagg ttttaagaaa
     5221 ggtttaaata tagaattaaa gccacttgaa ttaacagaag ctcaattggc agaagtggaa
     5281 gaattaactg aaaaatatcg ttcagatgaa tggatgttta gaaagtagtt tttagaataa
     5341 aaaatggaag cacgtagact attttaccta caagtgcttc cattttttta tctattttct
     5401 tctttgattt cttttatact tacgcattgc tttgcgatat ttgcgttggt cttcagttaa
     5461 gatgaagaaa taataaatca gataaatgat accagctatt acagcaaagc tgactaacat
     5521 tctgacgatt gaaaaaataa aggcatctag attcattact aatccaatcg cagcaataat
     5581 aagtacaaga taaaatatta tattacgcat taaaagactc ctatcatgat tttgcattta
     5641 agtttaattt tgacaacgct tgttgtcctt ttgtaagttg ttttttatct ttattttgat
     5701 agccattttt aatatcatca aatgcatgat tcaaatcaga gtttaattta tcgatatttt
     5761 tagacgtttt tttatcatca tcactaagtg ataaaccatc gatagcatct ttgtatgaag
     5821 tgtgagcact ttgaatatca ttagaaattt gatttaattt cgattgaaca ttcttatcgc
     5881 ctttgttttt acttacatct ttctcaatat cgttatagga tttcaaagct ttagcaactt
     5941 tgtcgtagta tgttgatgta acattcatca atttaatttt cgtattatta gatgcagttt
     6001 ttttactgtt ttctttatcc ttttcaagcc ctttaatttt agatttagaa tcactaattt
     6061 gttgattaag ttcttgaata tctagtttga gttgatgatt atcttctctt aaatctgttg
     6121 ttttttcttc taatggtgct aaattttgtg atccacatcc agctaacaat aatgttgcgc
     6181 caacaattga aaccaatttt ttcattacta tctccctaaa atcaatatct attatttttt
     6241 atccttatta tgatattatt ttaatgtata aagttgaaat gcacaaacgt ttcactagga
     6301 ggtaataaga tgagcagaat aacacaagtg catcgtattt tagaacaaaa acatttagat
     6361 gcgattatca ttctttcgga ttacaataga agatatttat caggttttac tggtaccagt
     6421 ggagcactta tcatatctaa agataaacaa tatttgataa cagactttag atatattgat
     6481 caagcaacta agcaggcgtc aaattatgaa attattaatc gtaaatctac tattattggt
     6541 gagattaaag aattgctaca ccaagaaaat tttgaaaatg taggttttga ggggcaccat
     6601 gtaagttatg atacatacct tgaattaaat aaaagccgta tatcattaat aagcatttct
     6661 aatactgtag ataaaattag aaacgtcaaa gatgctgacg aaattgcttt aattcaaaaa
     6721 gcagctaata ttgttgatga aacatatgaa tatattttaa ctgttgtaaa agcaggtatg
     6781 actgaaaaag aattaaaggc aatattagaa agtaaaatgc tagaattagg agcagatgga
     6841 ccatcattcg atacgattgt agcatctggt catagaggtg tattaccaca tggtgttgca
     6901 agtgacaaaa ttattgaaaa aggcgacatg attacattag attttggcgc gtattataac
     6961 ggctattgtt cagatattac tagaacattt gctattggag aaccagatcc taaactgaaa
     7021 gaaatttatc aaatagtact tgaatctcaa atgaaagcaa ttaatgagat tagacctggc
     7081 atgactggtg cagaagctga tgccatttca agaaactatt tagagtcaaa agggtatgga
     7141 aaagaatttg gacattcact aggacatggt attggtttag aaatccatga agggccaatg
     7201 ctggctcgta cgatacaaga taaacttcaa attaacaact gtgttacagt agaacctggt
     7261 gtttatatag aaggtttggg cggtataaga atagaagatg atattttaat tacagaaaat
     7321 ggttgtcaag tctttactaa atgcacaaaa gaccttatag ttttaacata agcgtgtaaa
     7381 atgaggagga aactgaatga tttcggttaa tgattttaaa acaggtttaa caatttctgt
     7441 tgataacgct atttggaaag ttatagactt ccaacatgta aagcctggta aaggttcagc
     7501 attcgttcgt tcaaaattac gtaatttaag aactggtgca attcaagaga aaacgtttag
     7561 agctggtgaa aaagttgaac cagcaatgat tgaaaatcgt cgcatgcaat atttatatgc
     7621 tgacggagat aatcatgtat ttatggataa tgaaagcttt gaacaaacag aactttcaag
     7681 tgattactta aaagaagaat tgaattactt aaaagaaggt atggaagtac aaattcaaac
     7741 atacgaaggt gaaactatcg gtgttgaatt acctaaaact gttgaattaa cagtaactga
     7801 aacagaacct ggtattaaag gtgatactgc aactggtgct actaaatcgg caactgttga
     7861 aactggttat acattaaatg tacctttatt tgtaaacgaa ggtgacgttt taattatcaa
     7921 cactggtgat ggaagctaca tttcaagagg ataatctcta atttgttaac aaatagcttg
     7981 tattcactat actgatttaa cgtaagatat tccaaataag tctcataaag ctattgccta
     8041 aaatgattat aggttatatg ctgatatgag gctttttatt ttaaaaataa attttaaaat
     8101 ataaaacgaa tcgtctaatt ggtagtgaaa aaacatagac atgagtatgg gtatatatct
     8161 acatatcata ttgttgagta taaaacataa tagcttataa aaat
//
LOCUS       DAHHRR010000105         7966 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.105, whole genome shotgun sequence.
ACCESSION   DAHHRR010000105 DAHHRR010000000
VERSION     DAHHRR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.105"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(8..1057)
                     /locus_tag="NBI42_002099"
     CDS             complement(8..1057)
                     /locus_tag="NBI42_002099"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCD1778494.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKSTGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRSLNIHRG"
     gene            complement(1088..2035)
                     /gene="corA"
                     /locus_tag="NBI42_002100"
     CDS             complement(1088..2035)
                     /gene="corA"
                     /locus_tag="NBI42_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCD1778495.1"
                     /translation="MALTIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNRVVYKYMNALDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDNVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            2132..2623
                     /locus_tag="NBI42_002101"
     CDS             2132..2623
                     /locus_tag="NBI42_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCD1778496.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIANYYKNQPTITWPEQLGHKIAIPYKG
                     ALK"
     gene            2620..3039
                     /locus_tag="NBI42_002102"
     CDS             2620..3039
                     /locus_tag="NBI42_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778497.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSESH"
     gene            complement(3147..4067)
                     /locus_tag="NBI42_002103"
     CDS             complement(3147..4067)
                     /locus_tag="NBI42_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1778498.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNK"
     gene            4275..4913
                     /locus_tag="NBI42_002104"
     CDS             4275..4913
                     /locus_tag="NBI42_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778499.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKEAVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADALKKYYLNVPPDQVITEADAIHNGIILLGCSIGLIVISLFFRNRMPRLK"
     gene            complement(5160..7091)
                     /locus_tag="NBI42_002105"
     CDS             complement(5160..7091)
                     /locus_tag="NBI42_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCD1778500.1"
                     /translation="MTTTFIISYIVLALIIVGVINLLLIRSRRKAKRQQKEQHFATRQ
                     SNQSKFKASDLDRTTDQSSQHTAREEARIDNKDNQNQVSLTDHAKGSEQDQELFNDNK
                     DDEAFAAKNPESEEYKVNEKIKKEHKNYIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PTIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILVGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKAPMLNLEVLKFP
                     TFTLTTVINMVVMMSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQNIDADNSINHE"
     gene            complement(7104..7751)
                     /locus_tag="NBI42_002106"
     CDS             complement(7104..7751)
                     /locus_tag="NBI42_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCD1778501.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYITTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMELKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASVKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLDSEPSKQVVPGMNAEVKIHKN"
     gene            7872..>7966
                     /locus_tag="NBI42_002107"
     CDS             7872..>7966
                     /locus_tag="NBI42_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1778502.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICA"
BASE COUNT         2624 a         1459 c         1130 g         2753 t
ORIGIN      
        1 cctctattta tcctcgatgt atattcaagc tacgttgttc tatccatgac aatatttccg
       61 gactaaatac gatttgtttt tgtgttaagt cgtcaatatt tttagcatct aacatcgtca
      121 ttattgattt catgtgttca ataaatgatt ctacataagc tactgtatgt gcaatgccat
      181 tattttcaac ttgatttaaa aacggacgtg acataccagt tgactttgca ccaagtgcta
      241 aacttttaat tgcatcgagt ggtgtacgta aaccaccact cgcgaaaact gaaatttcgc
      301 tttgataagc cgttgtttca agtaatgact caactgtaga ctgtccccat gatgataagt
      361 aatccatatc tttatttgca cgacgttcat tttcaatatc tacaaagtta gtaccacctt
      421 tgccactaac atcgacatac ttaacgccta tttgttgtaa gtcatgcatt aattctttgc
      481 tcataccaaa tccaacttct tttataatga ctggaacaga cactcgtgat acaatcgacg
      541 ctatattatc taaccaagtc acaaattcac gattcccttc aggcataact aattcttgag
      601 gagaattaac atggatttgt aacgcttgtg cctcaagtaa ttcaactgct tccaaagcct
      661 tttctactgg tacgtccgca ccaacattgc taaaaatcat gccttcagga ttcatttttc
      721 gcgcaatcgt aaacgtctca gccatgcgtg gatttctcaa tgccgcatgt gttgatccaa
      781 ctgccatcgc taagccagtt tctcttgcaa ctacagctag cttttcattg atgtttttcg
      841 tccactcgct accacccgtc attgcattaa tataaaccgg atatgccatc gttaagtcag
      901 gtgtctgtga tgtcaaatcg atatcattta cattaattga tgggatagaa tgatgcacaa
      961 aacgcatctt atcaaaatct gaatgcattg cgtcagattg ggccattgct atttcaacat
     1021 gttcattttt tctctgttct ctttgaaaat cactcatgat taaacctacc ttttcgtcat
     1081 ttctaattta aaaccatttt ttacgtctga aaaagatgat taatacgaca gctataacaa
     1141 acattacagc taagcaaata taatatccat aatgtaaatg taatgccggc atatttacaa
     1201 agttcatacc ataaatccca gctatgaatg ttaacggtga aaatataact gatactaacg
     1261 ttaatacttg cataatacta ttcattctaa atgacgtgta tgactcaaaa ttttctcgta
     1321 tttcgtttgt catttcttga gcagtacgaa tgatattacg ttgcttaatc aagtggtcat
     1381 cgatatgttg aatgtatagc gaatgtttat tatctataat caaatcacca ttttgtttca
     1441 ttgtatcaat tagctcttgc ataggaaaca gtacacgttt tactttaatc aaatccgaac
     1501 gtaacttaaa gacattatcc atgaccattt tattaaagcg atcatctaca tgtcggtctt
     1561 caaaatgata aacactatct tcaagtgcat atacaaagtt gaaatattta tcaaccatca
     1621 tatctaaaat taatatgacg acatctgcac aatctaattc tgcatctaat gcattcatat
     1681 acttatagac tactctattt aatgattcca acgtttgatg atgatatgtt actaatacat
     1741 tgtcttgtat aaaaatattt agtgctattg gtgaatagtt tgaccccata atactatgga
     1801 atactaagta ttgataatct ttataagatt tatatttagc tcgtggcata ccgttaattg
     1861 catcatccac ttctaaatca ttaaaattaa aatgtgcttt aaaccattca ttttcttgtt
     1921 cattcggttc atcaaaatca taccaaacaa tagtcgcatc ttttggtatc tctttgatat
     1981 catcaactac tttaaacggt tcatatgtag tttgataccg tatcgttaaa gccatcgata
     2041 ctccccctaa ataacgaatt ctctattatt ttatcatgaa ttaaataacg tgtatgtctt
     2101 aatttatttt agtatgatag tcactaagga gatggttatt atcaaacaac tttttacaca
     2161 tactcaaacc gtaacatctg aattcattga ccataacaat catatgcatg atgcaaatta
     2221 taatatcatt tttagtgacg tcgtgaatcg ttttaattac agccacggac tttctttaaa
     2281 agaacgcgaa aatttagcat atacgctatt tacactagaa gaacatacga catacctctc
     2341 agaattgtct cttggcgatg tatttactgt tactttatat atttatgatt acgattataa
     2401 gcggttgcat ttatttttaa cattaactaa agaagatggt acactagcat caacaaatga
     2461 agtaatgatg atgggaatta atcagcacac acgtcgttct gatgcttttc ctgaatcatt
     2521 ttcaacacaa atagcaaact attataaaaa tcaaccaact atcacttggc ctgaacaatt
     2581 aggacataaa atagcaattc catacaaagg agcattaaaa tgacagatgc attacaacaa
     2641 aagattcata ttgaattact agatttatta gatgatgtta agtttgaatt aacagaatta
     2701 aatgcacaaa aagggttata cattaacgga ccagcaaatc agctacttaa gcgtggcgtg
     2761 catatggctt atgttcaagg acaaaagcaa gctatcgata atattatgac tattgtggaa
     2821 caacagcttg aagatcaaca tttcctagaa cattatgata aatttcaaaa cgaagttgct
     2881 catcgcaatt atgataaaac agctaacttt gcagaactta gtgatattcc acgtcaattc
     2941 gataattttt tagatcaatt ttatcaaatt aaagggcaat actttatcat cacacatatc
     3001 aatacactta ttggtgattt tcactcagaa tctcattaac aattagtcta tataatcctt
     3061 gctatatttt caaaaacaaa acccaattgc gttttcatgt caaatatcat cttccatgaa
     3121 atcgtaactg ggtcaattgt atgttattac ttattttgtg ttacatcctc atctatcgat
     3181 ttggcaattt gtttaatagc tttatgtgat tgtctaattg gataaattgg aaaatcatgt
     3241 accatcttag gataatcata aaactcaatg tattgatgat gttgcaacat catttgttcg
     3301 aacagcttca tatcaggatg tgtcatttca cgtccaccac caaacatata aactggtggt
     3361 aatccttcta ttgtgccatt aatcggcgat atgcgcttat ctgttaatgg taggccattc
     3421 gcccattttt tcataatctc attgacacca aactgactta gaaccgcatc ttgttcgatt
     3481 aaagcgtccg aaatatcttt attagatagt gttgcatcta aaattggtga gattaaatac
     3541 aatttattcg gtaatggctg ttgattatct aaaagagatt gtacaaagga taatgccagt
     3601 gcaccacctg aaccatcacc catgactacg acattttgat ggcctacttc agatactaat
     3661 tgatcataaa cacgttgtat cgcttggaaa gtgtcgtcaa tatgaaactc tggtgtcttt
     3721 ggatagatag gcagtacaac ctcatataat gtacttaaag tgattttatc caacaatctc
     3781 caatggaacg gtgatggttg tagtgcattg aatccaccgt gaatatataa aattttctta
     3841 tcaatttgat gtctgaaatt aaagcgaaag acttgcatat catctaatga taatttttct
     3901 aaatttgctt taacatttaa tgttgaaggc tgcttatgtt tttttctatt ttcaatttct
     3961 cttttataaa aaaatctttc aacatcttga tcatttttaa acataatcga gcgattgtga
     4021 agcaaatatt tattgacaac gctattcata acacggtttc taatcaatgt cttaacctac
     4081 ctttatatat tttatgtatc caatgattgt ctatccccta cattctttgc caaaaaaagt
     4141 atataatgta gaagatattt tctttttcac tttcaaattt aagactacaa ttgaacagtg
     4201 atttttcatc attataacag acaactagac atattgataa gtaaagaaaa gaactttata
     4261 cggaggtacc ttgcatgaca aatccaaatc aacgattaga accatttgat gagacatttc
     4321 aacaaccgaa tattcatcgt ggtaagcgat atggtaagaa aaaacgttca ttggtaagca
     4381 tgattattca aatcattgtt gttatattaa ccgcaatcgc tggatacagc atgtggaaac
     4441 aacccatttt taatattgtc ttcaccaaag aagctgtaga ttttgatggc attaaaaatt
     4501 ttaacgagac aattggaaaa atgcatcaat taaatattaa tttgggtaat atcaatgatt
     4561 tacaacaagg aatcgatcgc ttaattttgg tctttaatgg tttctttatt ttatgtatcg
     4621 ttagcctagt cataacaata ctgacaattg tatttaatcg cacagttcta aaagtggtaa
     4681 atattttagt gttaacaatt atgttagtca ttacattgta tttcagttat attattaaag
     4741 cactcgcaca agaaattgca gatgctttaa aaaaatatta tttaaatgta ccaccagatc
     4801 aagtgataac agaagctgac gctattcata acggcatcat tttactgggc tgtagcatcg
     4861 gtttaattgt tataagctta ttcttccgta atcgcatgcc tcgtttaaaa taatgttata
     4921 tagtagggat tgggacagaa atgttatttt cacaaaattc atttcgttga cccacttcct
     4981 gctttttctt tattaatgac ctactacgtc aatgccaaat tttaatataa ttcctctaac
     5041 attctacaaa tcaacaaata aaaaaagtat cgataaccat atattggcac cgatacttta
     5101 aaaatcatgt ccccacaaca taatttttga ttagtcacat ttcaattaat ttattttatt
     5161 tattcatgat tgatactatt atctgcatct atattttgag ccgtagcttt tgcttttttg
     5221 ttgctttgta aaaataagca taaaattaaa gcgatgatgc taaatatcgt tgcaactata
     5281 aatgcatcat taatcccttc aactgttgct agtttattta caaattgtaa taacactttc
     5341 attgcgcctt cttgaccacc atattgcgat gctaattcac gcatatgatc ttgtacaaca
     5401 ggattcgttt tatctaactc ttccccaaaa gctgataaat gttgtgtagt ttgcgttgtc
     5461 attacagtaa ccaagattgc tgttcctata gaaccagcta gttgacgcat cgtatttaag
     5521 aaggcattac catgggaggc aagtcgtccc ggcaacgcat taatagctgc tgttaccatt
     5581 ggcatcataa taaatgccat accaaatgaa cgtagtacat agatgcccat gattgtcata
     5641 tatggcgtat ccatattcaa tttagttaat tcccatgttg cataagtcat gacagcaata
     5701 ccaaaaatgg ctaaaggttt taaaccaatc gtgtctaaca acttaccagc aaatggtcct
     5761 agtagaccca taattagaga accaggtaat aataacaatc cggaatctaa tgctgagaaa
     5821 ccacgtaaat tttgtaagta aattggtaat aaaatcatac caccatacaa gctcatcatt
     5881 acaaccatat taattactgt tgttagtgta aatgttggaa atttcaatac ttctaaattc
     5941 aacattggtg ctttcattct taattctctt attacaaata gaataataaa gataatacca
     6001 atcgcaaaca ttgtttctat ctcaacagaa ccccagcctt tgttccccgc ttctgaaaag
     6061 ccatataaca aagcaccaaa accaattgta ctaaaaatga tacctgggat atcagcttta
     6121 gggtttgttg tgtattgata taacttaaac catacaaaac caactaagat tgcaataatt
     6181 ccgataatga acataccgta aaacatcaca ttccaatggt aattttgtac aatataacct
     6241 gataatgttg gaccaattgt aggtgctaaa atcattgcga tacccattgt acccatggca
     6301 gcaccacgtt tttcaggtgg ataaattgta ataataacaa ttgaacctaa tggcattagt
     6361 acacctgcac caatggcttg taatacacgt ccaaccatca tgattgggaa attcattgaa
     6421 atcgcacata ttaatgaacc aattgtaaag agtactaacg caactaaaaa taattttcga
     6481 tatgaatatt tattaaatag atacgccgta attggtatta aaataccgtt tactaacatg
     6541 aatcccgtca tcaaccattg ccctgttgac gcagaaatat taaattccgt attaattttt
     6601 ggcaaagcaa catttaataa tgtttggttt aaaatcgcaa taaacatacc gaataataat
     6661 gccgctaata ttttaccgcg tgaaacacct tcaccaaaaa tatagttttt atgttctttt
     6721 ttaattttct catttacttt atattcttct gattcaggat ttttagcagc aaatgcttca
     6781 tcatccttat tatcattgaa taattcttga tcttgttcag accctttcgc atgatcagtt
     6841 agactaactt gattctgatt gtctttattg tcaatacgtg cctcttcacg cgccgtatgt
     6901 tgtgacgatt gatcagttgt tctgtctaaa tcacttgctt taaatttaga ttgatttgat
     6961 tgacgtgtcg caaaatgttg ttccttttgt tggcgttttg cttttcttct tgatcttatt
     7021 aataataaat tgataacccc aacaataatg agcgctaaaa caatgtagct aataatgaag
     7081 gtcgtagtca tttaatgacc cccttaattt ttatgaattt ttacttcagc gttcattcca
     7141 ggaacaactt gtttagacgg ttctgaatct aacgtaattt taacaggtat aacttgagat
     7201 actttagtat agttaccatc actatttgat gatggcatta atgaaaaact agcagcagta
     7261 gcttttccaa tactatcaac tttaccttta acagaagctt tttgaccgtc aatagtcaca
     7321 tcaacatctt taccaacttc aacatcttta atatcttttt cgtcaatatt tgcagttaca
     7381 tataaatcat ctaaattgta agcataagca attggattgc ctgcttgtac catagaacct
     7441 tccatgccat ctaatttagc aattgtacct ttttgtggca ttttaagttc catatctttc
     7501 gtttcgccat cttgaccttg aacagtaact gttgctactt tatcaccttt atcaagttta
     7561 tcaccttgct taacatttag tgatttgatt tgccccgaag ctgggcttgc tattttaatt
     7621 tgatcgccat ttactttagc attatcagtt gttatataac ttgttgtttt attccagaaa
     7681 taaaaaccag caatcccaat tgctaacagt acaacaaccg taatgacatt aattaatatc
     7741 atctttttca tgaaacattt cctcctattt gcttttaaaa ttcactttat ttattataga
     7801 gacatgatga ctaaaaaaac accaacaatt tttataagag tgttggttaa aattcacaaa
     7861 ggtgtaacaa tatgaaacga caagccaaaa tagaaattca aaacgcactt gttgatttga
     7921 tggctgaata tccttttcaa gaaatatcca ctaaaatgat ttgcgc
//
LOCUS       DAHHRR010000106         7936 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.106, whole genome shotgun sequence.
ACCESSION   DAHHRR010000106 DAHHRR010000000
VERSION     DAHHRR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.106"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(58..207)
                     /gene="rpmG"
                     /locus_tag="NBI42_002108"
     CDS             complement(58..207)
                     /gene="rpmG"
                     /locus_tag="NBI42_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD1778503.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            complement(320..2395)
                     /locus_tag="NBI42_002109"
     CDS             complement(320..2395)
                     /locus_tag="NBI42_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCD1778504.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVSINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     DDKNKDKDK"
     gene            complement(2514..3113)
                     /locus_tag="NBI42_002110"
     CDS             complement(2514..3113)
                     /locus_tag="NBI42_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCD1778505.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            complement(3389..3799)
                     /locus_tag="NBI42_002111"
     CDS             complement(3389..3799)
                     /locus_tag="NBI42_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD1778506.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            complement(3786..4649)
                     /locus_tag="NBI42_002112"
     CDS             complement(3786..4649)
                     /locus_tag="NBI42_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD1778507.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAVIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLVITMAYQKMRMK
                     FKKGANINEYK"
     gene            complement(4691..5476)
                     /locus_tag="NBI42_002113"
     CDS             complement(4691..5476)
                     /locus_tag="NBI42_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD1778508.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            complement(5602..6492)
                     /locus_tag="NBI42_002114"
     CDS             complement(5602..6492)
                     /locus_tag="NBI42_002114"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCD1778509.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            complement(6502..7848)
                     /locus_tag="NBI42_002115"
     CDS             complement(6502..7848)
                     /locus_tag="NBI42_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1778510.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFFLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
BASE COUNT         2441 a         1468 c         1073 g         2954 t
ORIGIN      
        1 acctcttatt ttgagatttc acctttttta ttgttaattt aaatttaatt ttaatgatta
       61 ttttgtttca cggtgtaaag tttgtttgtt ttcacgtgaa cagaatttct tcatttcaac
      121 acgttctgga ttatttcttt tgttcttagt tgtaatgtag tttctgtcac cacattccgt
      181 acaagctaaa gttacgttta cgcgcatggt tattcctcct tatcatttct aaatacgact
      241 ttattattat accaacatct ataatatttg caaagttaaa attttacaac catgcgctac
      301 acaatcgtca ggctaaattt tatttgtctt tgtctttatt tttatcatct ttacctaact
      361 gcttaaagta gtagttaatt acatctctac ctaagtctcc acctgttaac catggtggtg
      421 gtacaggctg atttgtatat acaattgaaa acgctaattt tggatcatca attggcgcgt
      481 atcctatata agtagagtta actcttggct ctccgttttg gaacacttca gcggtacccg
      541 ttttaccagc agtagggact actgtatctt taaaactaac atatccagta ccatctttat
      601 cattaaatgc cattttgaat ccttcttgaa tttgtttgat ttctttttca gtattattaa
      661 ccttgttcaa gacagtgcca ttaattttct tcttgagtgg accaacctca tctttattag
      721 ttgattcatg aatcgttaat ccaatgtgtg gctgtattct ataaccatca ttcgctatag
      781 ttgaaacata ttgtgataat tgtaatggtg tataggtatc atattgacca attgataaat
      841 ctagataatt acctggatta tttgttaatg gttcgatttg acctcttgtt tcatttggta
      901 aatctatccc tgttttcaca cctaagccta cttgatttaa tcctcttctt agcttttggg
      961 caggtgaact tatgtctgaa ggtaaagcca taccagaata ataagggtct cccgctaatt
     1021 ttaatgctgt tttaaacata tatacgtttg atgaatgcat caaagcttgc ttatcattaa
     1081 tagatacatg cccgttttta ttaaagtatg atcgttttgt caaaccacct tggaaatgta
     1141 atggttcatc gaccattgtt tctccaactt tgatagcttt attctgataa ccagctaata
     1201 atgttccacc ttttacagaa gatccaaccg caaattgaga agtaaacgta ccaatgtcat
     1261 aatcagtcat tttaccactc ttattaatct gctttccggc aagcgcaaga atgtctccat
     1321 ttttaggatt ttgtacaacc atcatagcat tatccatatc ttttgcacct tggctgcgaa
     1381 gcttcttaat ttgtttatcc aataatgctt ctacttcttt ttgaagatct atatcgatcg
     1441 ttaatttcaa atcttgaccg cgagcgccag gatttaacac ttctgaagat gtaactttac
     1501 cagatttgtc cgttgtgtat ttcatttctt tcttcttacc acgcaataca tcttcatatt
     1561 gatattctag gtaagatttt ccaacacgat cattgcgtga atatcctttg gataagtaat
     1621 gttctgtcaa ttcttttgga ataccttcag caggtgtcga tacatctccg aatatacctc
     1681 ttaaagtgtc gccatatggg tattttctat cccaatccat agacgtgtta acacctggta
     1741 atttggaaag ttgctgagaa actgctgcat actctttttc actgacatct tcatttttta
     1801 tcatttgtgg atctaaaact gttcctgcat tcatctctcg aaaaatggct aaaacttgta
     1861 aatctttaga agacaattca tctaattgtg attttccgat tttcgataac agttgtttat
     1921 catattgatc ttgtttaata cttccatctg ctaacatagc ttgttctttt gtcatcattg
     1981 cttttgcttt tttaggatgc aactgaatcc agaaatcttt cttatcacgt tctgtaatct
     2041 tcttagtatc catcttgatt agcttcgata acttttcagc cgtatccaac atttccgatt
     2101 gtgttgtttt tcgaccccta gtatatgtaa tagccatttt agaagcatta tcaactaaaa
     2161 ctttcccatt tctgtctaaa atacgacctc tcggcacaga ctcattcact gtaatgtttt
     2221 catcattttt tataatttgt ttataatgtg agccttgtgc gatttgtaaa taacctaaac
     2281 gtagtactag tactgcaaaa ataaatacaa tcacaccaaa tataaagtta attctcttgt
     2341 taattgtatt ttgaacgatt tcatcatttg atttttcttt tagtcttttt aacaaaacta
     2401 cctacctcta ttcaaagtct ttcactttaa atcatatatg aatttagaaa ttatttctat
     2461 ctttttgaca aaaaaaacgg tctcatttaa gagaccgaac aagtaatcat actttatttt
     2521 gttgcattat ataattcgtc aactttttcc cagttaacta cattccaaaa tgcgccaatg
     2581 tagtcagggc gtttgttttg atattttagg taataagcgt gttcccatac gtctaaacct
     2641 aaaataggtg ttttaccctc agttaatgga ttatcttggt ttggtgtagt cacaatttct
     2701 aactggccat tgtttacgac taaccaagcc caacctgaac caaagcgtgc agctgctttg
     2761 tcagcaaatt cttttttaaa ttcttctaaa gaaccccatt gttctttaat tttttctact
     2821 acagtacctt tttcttctga gtttggtgaa agtaactccc agaataatga atggtttaaa
     2881 tgtccaccgc cattattacg tacagcagtt tggatgttag ctggtacact gtctaaatta
     2941 gcaacaattt cttcaataga tttagattct aaatctgtac cttctactgc agcatttaat
     3001 ttcgtaacat aagtgttatg atgtctgtca tggtgaattt ccatagtttc tttgtcaaaa
     3061 tgtggttcta atgcatcaaa tgcgtatggt aattttggta attcaaaagc cataaataat
     3121 catcctccta aaatgtctgt aagtaaataa taacaagcag tgactggttc aacaaagaat
     3181 ttgcttaaat tctactactt attattttct ctactcattt aatataactc aaatcaaaaa
     3241 taattaaaca ttttgtatat aaaaagttaa cagatttgcc ataaaatcat acgaacggag
     3301 tatgaaatga acctttatct tctataattt aaaaaatgag atttatgcat acatctgacc
     3361 aaatgtgcat aaatctcatt tctttatatt aatcttggca agactcacat acaccataaa
     3421 cttcaagttt gtgtttgtga atattaacac caggaagtga taattttatc tgatctattg
     3481 gacaataatc tattaccttt gtatctccac acttttcaca gataaaatga tgatgatgat
     3541 ggtttgtaca agcgattcta aacttcattt caccatcaag ttctgtattt tcaataatcc
     3601 ctaaatcttt aaataagtgc aggtttctat atattgtgtc gaatgaaatt ccaggataat
     3661 tttcatccat aacttgttgt atatactttg cgtttatata cttatcttct tcgacaaaaa
     3721 tatctaacat atctttacgt ttatctgtat attttaaacc gttctctttt aaaattttaa
     3781 tagcatcatt tgtattcatt gatattagct ccctttttaa acttcattcg cattttctga
     3841 taagccattg taatcacaag taaaataact agtagaacta caattacacc acccggagaa
     3901 atgtccatat agaaagctag gactaagcct aatattactg ataattcacc taaaaataca
     3961 cttagtaata tcaattgctt aaaacttttt gttattcgca tacttattgc aattggtaac
     4021 gtgattaacg cacttactaa caggatccct acaacacgca ttgaggcaga aataaccatc
     4081 gctacaataa caataaataa aaattgaatc catttaggaa tgccaatgac tttactatat
     4141 tcctcatcaa atgacaatat aaataattct ttataaaaca atgtaataaa cagaaccact
     4201 atgacggcaa tgacaataat cgttgttaaa tcacttatat tcactgcgct tattgagcca
     4261 aataacaatc caacaatttc ttgattgaac ccatcagcta atgaaatgaa gattgcactc
     4321 aaggcgatac cagcactcat tataattgga atagcaattt cttggtaagc agtgtatgac
     4381 gttcttaatt tttcaattag aagcgcacct actattgcga ataagattcc aaaccacatt
     4441 ggattaataa atactagtgt tggcataata gtaagtaaaa acataccgaa ggatatacca
     4501 cctaaagtta catgacttag agcatcagct ataagtgata gtcgtctaac aacgataaaa
     4561 gcaccgatta gaggcgcaat aaaacctatc aagataccac taattaaaga gtacctcata
     4621 aaatcaaaat tcaataatgc atctatcaat tgtgacacgc ctttccattt taaataaact
     4681 caaatcttta ttaattacaa cattctcgat tatgctgatg atcgacaaaa cgtacaggat
     4741 gtccataaat ttttgaaatt tcaacttcat caagtgattt aaactcatca gttgtaccat
     4801 ggaaatgcaa atgcttattt aaacatgcta cttcagtagc agtatctgct acaacaccaa
     4861 tatcatgagt aactaagata atggtgatac cttcttgttt taattgatct aaagtattat
     4921 aaaattcact tacatgtttt gcatcaatac cattcgttgg ttcatcaagt actaatactg
     4981 caggttctga aatcaatgct cgagcaatca ttacacgttg ttgttgacca cctgataatt
     5041 ctgctatatt tttatgaatt aaatcactta tattcagtct ttctagtact ttaatcactt
     5101 tttcattatc tttgctatta aatgtttgga aaagacgttt tgtctttgtt aatccgctta
     5161 aaacaacttc tttaacactt gctgggaaac ctgaattaaa ggcatttgct ttttgtgata
     5221 catagcttaa tttaattgat gttttcttat ttttaaaatc aataccttca acaaaaatct
     5281 caccactttg taaaggtaat aatcctagaa tcaacttcaa taatgttgat ttaccagcac
     5341 catttggtcc aacaattgct aaaaattcac ctttatttat tttaatgttt atattttcta
     5401 acactttttt atgatcatag tagtaattga catttttcaa ttcaaagact ggtgtcatcg
     5461 tattctcacc tcgcattcaa ctatacaact cctagtaaca tatgtaaaca gtaatgttta
     5521 cgactcaaaa taagacaaaa taaagagata tgcccccttc aagtattatt tatcgcattt
     5581 cttgaagaga gcattatcat tttattgttg cataacctta ttttttaatt ctgggtcaaa
     5641 ttgctgttgt tttaacattt caatttcaag tttatatggc ggttttttat ttttcttatc
     5701 ttcaccaaca taaggtgttt ctaagatttt cggaatatct ttaaaactat catgatgcac
     5761 aatgtaattt aatgcatcaa aaccaatgta accgaagcca atattttcat gtcggtcttt
     5821 ttgagcgcca cggtcatttt tagaatcatt gacatgaaca actttaattc tgtcgactcc
     5881 aatgatttta tcaaattcat ttaatacgcc atcaaagtcc tctttcacat tatatccagc
     5941 atcatgcgta tgacatgtat caaaacatac tgataaacgt tcgttattat gaactccatc
     6001 aataatacgt gctaactctt caaacgaacg accaatctct gtacctttac ctgccatcgt
     6061 ttcaagcgca atacgtacat tattgtcatt cgttaaaact tcatttaatc cttcaataat
     6121 cttattaatt ccggcatcaa caccagctcc aacatgcgca cctggatgta atacaatatc
     6181 tttagcgcct atagcttgcg ttctttcaat ttcttgttgc aagaaatcta caccaagatt
     6241 aaacgtttct ggtttggttg tatttgcaat attaatgatg tatggtgcat gaacaacaat
     6301 attagataag ccatattttt ccatcacttc atgaccttta gttatattta aatcttcaat
     6361 acttttacgg cgcgtgtttt gaggtgcacc agtataaatc ataaatgttg tttcaccata
     6421 ttcatgcgct tctatagcag aaccttctaa catcttttta ccactcattg aaacatgtga
     6481 tcctaataac ataaaacaca cctaaccttt tttgttttgc ttacgttttt gtctattttg
     6541 ctgcttacta aattgtttac gctcttgacg tttcattttt tcaacttctt gtttaaattt
     6601 cttcttataa cctggtttaa ctttgttttt aattttactt cgaactttgt tcttcacttg
     6661 attagttaaa tggtcatctt tgcgcattct tgcttgacgc tgattgtgcg ctttaacttc
     6721 ttttaactca ccatctttaa tatcaacagt attgaaaaca aaaccgcgat cttctattaa
     6781 tgaaatattg tgttcttcat caggactata aagcgtaatt gctacacctt tataatttcc
     6841 acggccagtt cggccgactc tatgtgtaaa gaagtcaata tcatttggca catcaaaatt
     6901 gatgacatga ctaacacctt caatatcaat accacgagat gctaaatcgc tggcaataac
     6961 gtattggaat tctaaattac gtatacgttt catttgttgt ttacgttcac gtggcgttaa
     7021 accaccatga atcataccaa ctttaatacc agcttcattt agtgaacgtg ctaaatcatt
     7081 tgcattatct ctactattac agaaaataat acataagtat ggatttagta tatcaattaa
     7141 atttaaagtc ttttcaactt tagctgcacc tttagtaggt attaaaaaga attcgatgtt
     7201 ctttttattt tgttttttac tgtcgacagc tacatattct ggatgactta aatatttatt
     7261 taagaatggt tgtaactgtt gtggaattgt agcactaaac accgcaatat ttgcattatc
     7321 ttccaatctt gcagcaatgt aatctacatc ttcaattaat cctaagtcaa tcataagatc
     7381 cgcttcatca ataactaaat atgatgctaa gtgcacatgt aaatgtcccg ttttagctaa
     7441 gtcattaatt ctagtagggg tgcctataat caattgtggt tgtgcattac aacgttgtct
     7501 atctttctct atatctgtac caccaataaa aactttaact gaaacaccag ctttaaattg
     7561 gcttaaatgg ttcgctgcat cgtatagttg ttgtgcaagt tctcttgttg gtgcaactac
     7621 gattgcttgt ggttctttta tttcactatc aattaactgc attaatggta ataaaaatgc
     7681 atgagatttc cctgtacccg tttgagattg accaattaaa tttgttctct ttagtattct
     7741 tggaataatt cgattctgaa tttcagttgg tttttcaaaa ttaaggtctt tcacagcgtc
     7801 aattaaacta gattctagat taaattgttc gaatggatgt tttgccatga tttggcctcc
     7861 ttatatgttc atctttacta ttataaagta taaatgtgct attttaaata aaaatattaa
     7921 aacctcgaaa tttcaa
//
LOCUS       DAHHRR010000107         7891 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.107, whole genome shotgun sequence.
ACCESSION   DAHHRR010000107 DAHHRR010000000
VERSION     DAHHRR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.107"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            74..1261
                     /locus_tag="NBI42_002116"
     CDS             74..1261
                     /locus_tag="NBI42_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778511.1"
                     /translation="MSYHWFKKMLLSTSILIFSSSSLGLATQTVEAKDNLNGEKPTTN
                     LNHNVTSPSVNSEINNNETGTPHESNQTGNEGTDSNSRDANPDSNNVKPDSNNQNPSP
                     DSKPDPNNPNPGPNPKPDPDKPKPNPEPKPDPDNPKPKPDPKPDPDKPDPKPDPKPDP
                     DKPKPNPNPSPDPNQPGDSNHSGGSKNGGTWNPNASDGSNQGQWQPNGNQGNSQNPTG
                     NDFVSQRFLALANGAYKYNPYILNQINKLGKEFGEVTDEDIYNIIRKQNFSGNAYLNG
                     LQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGEIGSMPDLKKPEDKPDSKQRSFE
                     PHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMMVVFSIMLFLFVKRNKKKNKNES
                     QRR"
     gene            complement(1286..2029)
                     /locus_tag="NBI42_002117"
     CDS             complement(1286..2029)
                     /locus_tag="NBI42_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD1778512.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLSFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     YTQIFGTQQTSPNTERLIVIARKIPLFIFFVSIVGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFAIATTVSSLIFALAHNDYKFIPIYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            2204..2488
                     /gene="groES"
                     /locus_tag="NBI42_002118"
     CDS             2204..2488
                     /gene="groES"
                     /locus_tag="NBI42_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCD1778513.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIV"
     gene            2563..4179
                     /gene="groL"
                     /locus_tag="NBI42_002119"
     CDS             2563..4179
                     /gene="groL"
                     /locus_tag="NBI42_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCD1778514.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDATIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(4715..6022)
                     /locus_tag="NBI42_002120"
     CDS             complement(4715..6022)
                     /locus_tag="NBI42_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCD1778515.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLITELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLIEKIGNSFFQSVTTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISD
                     KHIKLSIVTINISFLFITIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYH
                     GLTKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL"
     gene            complement(6464..7687)
                     /locus_tag="NBI42_002121"
     CDS             complement(6464..7687)
                     /locus_tag="NBI42_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCD1778516.1"
                     /translation="MTTFSEKEKVQLLADIVELQTENNNEIDVCNYLKNLFDKYNIKS
                     EILKVNEHRANIVAEIGYGSPILALSGHMDVVDAGNHDNWSYPPFQLTEKDGKLYGRG
                     TTDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKKQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIEPFFQNIINDVDSQKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVDKDDIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIKYLKEK"
BASE COUNT         2859 a         1217 c         1358 g         2457 t
ORIGIN      
        1 ataaatgcat tttcaacgca tatttattat acttagatta atacttacat gaaaaaggga
       61 ggtgtctcgt gaaatgtcat atcattggtt taagaaaatg ttactatcaa caagtatttt
      121 aatttttagt agtagtagtt tagggcttgc aacgcagaca gttgaagcaa aggataactt
      181 aaatggagaa aagccaacta ctaatttgaa tcataatgta acttcaccat cagtaaatag
      241 tgaaataaat aataatgaga ctgggacacc tcacgaatca aatcaaacgg gtaatgaagg
      301 aacagattcg aatagtcgtg atgctaatcc tgattcgaat aatgtgaagc cagactcaaa
      361 caaccaaaac ccaagtccag attcaaaacc tgacccaaat aacccaaacc caggtccgaa
      421 tccaaagcca gacccagata aaccaaagcc aaatccggaa ccaaagccag acccagataa
      481 cccgaaacca aaaccggatc caaaaccaga tccagataaa ccagatccaa aaccagatcc
      541 aaaaccagac ccagataaac caaagccaaa tcctaacccg tcaccagatc ccaatcaacc
      601 tggggattcc aatcattctg gtggctcgaa aaatgggggt acatggaacc caaatgcttc
      661 agatggatct aatcaaggtc aatggcaacc aaatggaaat caaggaaact cacaaaatcc
      721 tactggtaat gattttgtat cccaacgatt tttagccttg gcgaatgggg cttacaagta
      781 taatccgtat attttaaatc aaattaataa gttgggcaaa gaatttggtg aggtaactga
      841 tgaagatatc tacaatatca tccgcaaaca aaacttcagc ggaaatgcat atttaaatgg
      901 attacaacag caatcgaatt actttagatt ccaatatttc aatccattga aatcagaacg
      961 atattatcgt aatttagatg aacaagtact cgcattaatt actggtgaaa ttggatcaat
     1021 gccagatttg aaaaagcccg aagataagcc ggattcaaaa caacgctcat ttgaaccgca
     1081 tgaaaaagac gattttacag tagttaaaaa acaagaagat aataagaaaa gtgcatcaac
     1141 tgcatatagt aaaagttggc tagcaattgt atgttctatg atggtggtat tttcaatcat
     1201 gctattctta tttgtaaagc gaaataaaaa gaaaaataaa aacgaatcac agcgacgata
     1261 atccgtgtgt gattcgtttt ttatattatg gaataaaaat gtgatatata aaattcgctt
     1321 gttccgtggc ttttttcaaa gcttcaggat taagtaattg gaatataacg acaaatccat
     1381 tttgtaacat atgaataata attggaacag caagtcgttt cgtccataca tatgctaatg
     1441 aaaaaatgac gcccatacca aagtaaatag gaataaattt atagtcatta tgcgctaatg
     1501 caaagattaa tgagcttaca gtcgttgcaa tagcaaatgc gacgatacgg ttacctttaa
     1561 ttgcattaaa taattcgcca aagattactt ttctaaatac atattcttct aatagaggac
     1621 caacgattga tacgaaaaag ataaacaatg gaatctttcg tgcaataacg attaaacgct
     1681 cagtatttgg acttgtttgt tgtgtgccaa aaatttgagt atatacaata ctaactacca
     1741 tttgataaat cattaccagt gcaaatccaa gcaatgccca tggaatgata tattttttag
     1801 gttctttaac ttctaattct aattttgttg gatttttaat ttttaaatta attaaaataa
     1861 tcgtcgtggc ggcgatcaaa aatagaacaa gttgtatgta aatgactgct ttagtcagtt
     1921 ctatgccact atattgtaca aatgataatt tttttacaat gagaagcggt aaaaattgag
     1981 acaatatata aataataaca gttagcagtg atgcccatag tcttgtcata attttactcc
     2041 aaatatttgt ttataattta ttttatcgta aataacttga agttacaaaa cttaattgaa
     2101 aggttatgac ttgaaatttt gaccaaattt gattattata aatgtatgtt agcactctat
     2161 aaacttaagt gctaaacttt aggtttttta aggaggaaca atcatgctaa aaccaattgg
     2221 aaatcgtgtg attattgaga aaaaagaaca agaacaaaca actaaaagtg gtattgtttt
     2281 aactgatagt gctaaagaaa aatcaaacga aggcgttatc gttgcagtag gaactggacg
     2341 tctattaaat gatggtacaa gagtgactcc tgaagtgaaa gaaggggacc gtgtcgtgtt
     2401 ccaacaatat gctggtacag aagttaaacg agataatgaa acatatctag tattaaatga
     2461 agaagatatt ttagcggtaa ttgtataata taaaactaaa ttcatagata aattataata
     2521 gataaagtta aacataacta aacatatgga ggtttatcat ttatggttaa acaattgaaa
     2581 ttctctgaag atgcacgtca agcaatgtta cgtggtgttg accaattagc aaatgcagtt
     2641 aaagtaacga ttggtcctaa aggacgtaat gttgtattag ataaagagtt tacagcacct
     2701 ttaattacga acgatggtgt aacgattgct aaagaaatcg aattagaaga tccatatgaa
     2761 aatatggggg ctaaactagt tcaagaagtt gcaaataaga caaatgaaat tgctggtgac
     2821 ggtacgacaa ctgcaacagt gttagctcaa gcaatgattc aagaaggctt gaaaaatgtt
     2881 acaagtggtg cgaacccagt tggtttacga caaggtatcg acaaagcagt taaagttgct
     2941 gttgaagcat tacatgaaaa ttctcaaaaa gttgaaaata aaaatgaaat agcgcaagta
     3001 ggtgcaattt ctgcagcaga tgaagaaatt ggacgttata tttcagaagc aatggaaaaa
     3061 gtaggtaatg atggtgtcat tacaattgaa gagtcaaatg gcttgaatac tgaacttgaa
     3121 gttgttgaag gtatgcagtt tgaccgtggt tatcaatcac cgtatatggt tactgattca
     3181 gataaaatgg ttgctgaatt agaacgcccg tacattttag tgacagataa aaaaatctca
     3241 tcattccaag atatcttacc tttattagaa caagtagttc aatcaaatcg tcctatctta
     3301 attgttgcag atgaagttga aggagatgcg ttaacaaata tcgtgctaaa ccgcatgcgt
     3361 ggtacattta cagctgttgc tgttaaagca ccaggatttg gtgatcgccg taaagcaatg
     3421 ttagaagatt tggctatttt aactggtgca caggtaatta ctgatgactt aggattagac
     3481 cttaaagatg caacgattga tatgttaggt actgcaagta aagtagaagt tactaaagac
     3541 aatacaactg ttgtagatgg tgatggcgat gaaaacagca ttgacgcacg tgtaagtcaa
     3601 ttgaaatctc aaattgaaga aactgagtct gactttgatc gtgaaaaatt acaagagcgc
     3661 ttagctaaat tagcaggtgg tgttgcagtt attaaagtag gtgcagcaag tgaaacagag
     3721 cttaaagaac gtaaattacg tattgaagat gcattaaact ctacacgtgc agcagtagaa
     3781 gaaggtattg ttgcaggtgg tggtactgca ttagtaaatg tttatcaaaa agtaagtgaa
     3841 atagaagcag aaggtgacat tgaaacaggt gtgaatattg tacttaaagc tttaactgca
     3901 ccagttcgcc aaattgctga aaatgcaggg ttagaaggat ctgtcatcgt tgaacgcttg
     3961 aaaaatgcag aaccaggtgt tggctttaac gctgctacaa atgaatgggt gaacatgtta
     4021 gaagcaggta ttgttgatcc gactaaagta acacgctcag cattacaaca tgcagcaagt
     4081 gttgcagcaa tgttcttaac aactgaagcg gttgtagcat caattccaga aaaaaataat
     4141 gaccaaccta acatgggcgg catgccagga atgatgtaaa actgtttata gttttgacta
     4201 attagaaaat tacattatgc acgagtatga ttcattctaa gtactaatta gaatgcacgc
     4261 aaatacattt taattacgag acgtcttcca tttggtgact aaacttatgg gaggcgtctt
     4321 tattatgtgt taatttgtta ttgagattca taatggtata taaatcattg tagtgcggga
     4381 gttatggtat tgccataata tgagagtaca tcaacttcta taagtaatgc atattgcgag
     4441 tgcaggaatg aatgagtact tgattaaaat ccttatgggt ggttgacata attaaaagaa
     4501 accacattta aaatttctta atcacaagcg gttaactagg tatagtttag ttttgagtag
     4561 atctttttat aggtgtactt gtgcatttta cttaattaaa gacataagac atttaatcgg
     4621 cctaaaataa ataaaaaact acctgcttag gtagtttttt aaatgtaata gattaaaata
     4681 ctagttcatt tcttgttaaa gatggatggt tatcttatag ataaatttgt cctttagtgt
     4741 agcggtaatt tttaggactt tttggtggta taaatgttct taataaagtt aatagtccta
     4801 ctttaccgca aagcataacg aatataataa ttattttagt aagaccatga tattctgtgg
     4861 taaggttcat acttaacccg actgttccaa atgcagaaac cacttcgaat aataacttga
     4921 ttaatgatat gttcggatta attatcgata atataaaagt aatgatagtg ataaatagaa
     4981 atgagatatt aatggtaaca atagatagtt ttatatgttt gtcagatatt tctttattga
     5041 atactgaaac attattttct ttacgtatat aatttagaac aaatataaac gccactgcaa
     5101 aagtagttat tttaattcct ccagctgcac taagaggggc accaccaata aacataagta
     5161 gcattaacat taaggcggta gatttgttaa tgcttgctat atcaatactg ttaaaacccg
     5221 ctgttcgtgt tgttactgat tgaaaaaaag aatttccgat tttttcaatt agtcccatat
     5281 gttgcatagt attaaactgt tctaataaaa agaatgtaat agctcctata attattagaa
     5341 tactagttgt agttaagact aatttagaat gtaaagataa tttactcaat tttttacaat
     5401 taataaagtc tattacgaca aaatgtccaa tacctccaaa tattatgagt attgagattg
     5461 taataatgac aattggatca ctagaataat ctattaagtt attcttaaaa agggcaaatc
     5521 cggcattatt aaaagctgat actgatgtga ataaacttaa aaataatcct ttgcctatac
     5581 caaattttgg tataaaagat aaacacaaac aaatcatacc aattaattca gtgattaaac
     5641 tataaatagc caagtgttta attagcttaa taacaccacc aggttcgtca atattccatg
     5701 taaccataat caagaatcta tttttcattg atatctttct atttaaaaat actagtgtca
     5761 atagggttac ggttacgata cccagaccac ctatttgaat taataatagt attactattt
     5821 caccaagtat attaaactgt gaccctatat caactggaga taggccagta actgtaaatg
     5881 cacttgaagc tataaatagg gcatctaaaa aagatattgg ctttttacca gtgaaaggta
     5941 aatacaataa aagtgcacct atgatagttg tagagaagaa tagcattaaa taaaaatata
     6001 aaggtttgtg gactttgttc attttaattg catactcctt tatattataa attaataatt
     6061 agataatatc ataaatgaaa atagaaatgt tactgatgtg tattactttt caattatagt
     6121 caggggcccc aacacagaga atttcaaaaa gaaattcaac agacaatgca agttggggtg
     6181 ggggccccaa cacagagaat ttcaaaaaga aattctacaa gcaatgtaag ttggggtggg
     6241 gcctcaacac agagaaatag gattccaaat ttcaacagac aatgcaatat atgtataaca
     6301 aatcagcacc ataatttcag tattacatca cttaatcaat gaaaatgttc ttaatcattg
     6361 tagttcattt acattcaaac aatattcatc gctatttcta ttaacttaaa tctcgcaaaa
     6421 aaaactgcca acaagacgaa aaacattgct gacagtatct ttcttatttc tcttttaaat
     6481 atttaatgga agcctcttta aaaatatcaa tatatttcag atacatatct ttttcaatat
     6541 attcatcgat ttgatgtgcc attaatggat tacctggtcc aaaaatggct aaatcaacat
     6601 tgtccttatt atcacctaag aaactagagg catctgttgc acctacaagc gccgaaacaa
     6661 atatatcgtc tttgtctaca taactagaag ctacatcttt aatcgtagta attaatttgc
     6721 tatttttatc gcttgttaca ggtcggtgat tgcttggaat atcgagtgaa agcttttggc
     6781 tatccacatc attaatgata ttttggaaaa acggttctat aaagtcgtta tcatactcag
     6841 gaactggtct tacgttaaat tcaagtgaag cttcatctgg tacagagtta aattgtttgc
     6901 caccatttat aatcgaacat acagctgtaa gaccagatgc ataatttgca tcctcttcag
     6961 aaatttcttt tccaatcaat gatttgaaca taggcgcaac atctaattca tgtttagtat
     7021 cttgtttttt aagctctgaa tatttttctt taaatagatt ataaaattca agcagtgtat
     7081 caattgcatt gtcaccaata aatggaactg agctatggac agctttacca gttgcagtta
     7141 ctttacatga catagacccc ttatgtgcat aataaattcc agatccagtt ggttcagcaa
     7201 taattaagcc atcgacatcg tctaaatagc ctttatcagc taataatttg gcaccttctt
     7261 gttctttctc ttcgccagca gtagccagta atctaatcgt tccatgaggc aattcatttt
     7321 gttcttttaa ttcgattaga gatacgacca aagccattaa accgcctttc atatctgtag
     7381 tgcctcggcc atataatttg ccatcttttt ctgtcagttg aaaaggggga tatgaccaat
     7441 tatcgtgatt tcctgcatca acaacatcca tatgaccact caatgcgagt ataggtgagc
     7501 cgtaaccgat ttctgcaacg atattggcgc ggtgttcatt aactttcaaa atttcagatt
     7561 taatattgta cttgtcgaat aaatttttta aataattaca aacgtcaatt tcattattat
     7621 tttcagtttg tagttcaaca atatctgcta gtaattgaac tttttctttt tcgctaaaag
     7681 ttgtcattaa gctcacacgt ttaaaaatta gtatatatta ctatataaac atattctttg
     7741 aacttaaaca ttattttcat taaagaatat agtgcgttat ttaattttga tttacagaat
     7801 taaacctcta tatacagaag tacgttgttt ttttattagc tcttttttgg aataaacggg
     7861 ttgcaataaa atttatttaa acaaaacaac t
//
LOCUS       DAHHRR010000108         7795 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.108, whole genome shotgun sequence.
ACCESSION   DAHHRR010000108 DAHHRR010000000
VERSION     DAHHRR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.108"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(132..959)
                     /locus_tag="NBI42_002122"
     CDS             complement(132..959)
                     /locus_tag="NBI42_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1778517.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPLPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
     gene            complement(962..1081)
                     /locus_tag="NBI42_002123"
     CDS             complement(962..1081)
                     /locus_tag="NBI42_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778518.1"
                     /translation="MMLISFVYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            1193..2194
                     /locus_tag="NBI42_002124"
     CDS             1193..2194
                     /locus_tag="NBI42_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HCD1778519.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPQLIN
                     TIEHLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVNAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANSYNNMHINIDTEKYASLQQIVQVLDRLTGEFRNVGTVI
                     QAYLYDSPELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            complement(2316..2780)
                     /locus_tag="NBI42_002125"
     CDS             complement(2316..2780)
                     /locus_tag="NBI42_002125"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCD1778520.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLAASGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTDVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(2793..3974)
                     /locus_tag="NBI42_002126"
     CDS             complement(2793..3974)
                     /locus_tag="NBI42_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HCD1778521.1"
                     /translation="MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVNDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     IFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            complement(3985..4617)
                     /locus_tag="NBI42_002127"
     CDS             complement(3985..4617)
                     /locus_tag="NBI42_002127"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCD1778522.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFDQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKDDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(4624..5667)
                     /gene="ribD"
                     /locus_tag="NBI42_002128"
     CDS             complement(4624..5667)
                     /gene="ribD"
                     /locus_tag="NBI42_002128"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCD1778523.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GNKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDDTLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTLIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(5794..5929)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(6148..7650)
                     /locus_tag="NBI42_002129"
     CDS             complement(6148..7650)
                     /locus_tag="NBI42_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCD1778524.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMQSYL
                     SMFTKKYPNISTNYNKVREIYTNSNIDETNLTYYICTTNSEQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECAEYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
BASE COUNT         2476 a         1438 c         1075 g         2806 t
ORIGIN      
        1 ttttcaagat atgatttgta taaatcaaaa catttcttac aaatcaaatg tgtataaaat
       61 gtgatataca ttctcaacct taaaaaagtt aaaaaaaaga tgagcatcta ctgtctcatc
      121 ttcttagaat attaaatttc tacaatttcg tcatcttcaa caataaagcc cattgtattg
      181 acgctgttat ttaagaaagt cagaatataa cgcattactt catcgcgttc tggctcattg
      241 tgaacctcgt ggtaaaaacc ttgccaagct ttaaaatata attcaggtgt ttgatatttt
      301 tctttaaact catcaattgc cctagtatca acaattaaat ccttcgttcc atacattaat
      361 agtgttggca atggttgaat gtcatgaata tgagccatcg tatctttcat cgtctcatta
      421 attgtattat accaatgata cgttgctttt tttaacatta aaccatcgtt aactgtttct
      481 tcaacaattt ctaaattacg tgttaaatct tttggttcta caccaacatt aaaacgtgtg
      541 tcttttgaaa ttttacctat atttgaaaca agtttatctt tacgattttt tccattcttt
      601 tgaagttcta gcataggaga aattaacatc attccctcga ttggcaattc tactttttca
      661 agtaaattta ataaaatcaa accgccaagt cctaccccta atacataagt aggaatttta
      721 tattcattag ctatctttaa ccagtctagc aaactttcgt gatacgtttg aaaattttca
      781 atttgtcctt tattagctct tgaagtttga ccttgaccag gcaaatctcc cataatcaca
      841 tgatagccat ttcttcttaa catcgtaata acatatgcat atcttcccgt atgttctaat
      901 atattatgag caataacaac gacgcctttc gcatcatttt cagcttccca cttccacatt
      961 attatactgc ccctttttca ttaatcttca ataacataat tatagcaaat tcactatgta
     1021 gatttctatt tatagtatta ttgttgtcca tattattata tacaaatgaa atcaacatca
     1081 ataatagtgt aattatacat aattattttt gattgttttt gatgaaaacg ctttctcgaa
     1141 tatttttttc atgctaaact tattgtaaac acaagggttt ggaggagtag caatggcact
     1201 attaaagaat ttttttatcg gattatctaa taatagtttt ttaaacaacg cagcaaaaaa
     1261 agtgggccca cgtttgggtg ccaataaagt cgttgccgga aatacaattc cacaattaat
     1321 caatacaatt gaacacttaa atgataaaaa cattgctgtt actgttgaca atttagggga
     1381 atttgttgga acagtggaag aaagcaatca tgctaaagaa caaatcttaa cgattatgga
     1441 cgcacttcac caacatggtg tcaatgcaca catgtctgtt aagttaagtc aattaggagc
     1501 tgaatttgat ttagaattag cttatcaaaa tttaagagaa attttactta aagcaaatag
     1561 ttataataac atgcacatta atattgatac tgaaaaatat gctagtttac agcaaatagt
     1621 tcaagttctt gaccgattaa caggtgaatt tagaaatgtt ggaacagtta tccaagctta
     1681 tctatatgat agtccagaac ttgttgataa ataccaagat ttgcgattac gtttagttaa
     1741 aggggcttat aaagaaaatg agtctatcgc ttttcaatct aaagaagatg tagatgcaaa
     1801 ctatattaaa attattgaac aacgtttgtt aaacgcacgc aatttcactt caattgcaac
     1861 acatgaccat cgcatcatta atcatgtaaa acaatttatg aaagaaaatc acattgaaaa
     1921 agatcgtatg gaattccaaa tgctctatgg ttttagatca gagttagcag aagaaatcgc
     1981 aaatgaaggc tataatttca ctatttatgt accttatggc gatgattggt ttgcgtattt
     2041 tatgagaaga ttagcagaac gcccacaaaa cctatctctt gctgtaaaag aatttgtgaa
     2101 acctgctggc ttaaaacgtg ttggcataat tgcagcttta ggagctacag ttatgttagg
     2161 tttaagtaca attaaaaaat tatgccgtaa gtagagcatg gcataaacaa taatttagga
     2221 gtctggaaca ataatcaatg ttctaggctc ctaaatgtta tattggcagt tctgaatcat
     2281 ttataaatct ttgattatga actcacaaca gcgctctatg cttttataga ttttaataaa
     2341 tttgccattt cgattgcact tacagctgct tctgcacctt tattacctgc tttagtacca
     2401 gctctttcta ctgcttgctc aatactttcc gtcgttaata taccaaaaat tactggtaca
     2461 tctgtttgat cattaacttt agatacacct tttgcaactt cattacatac ataatcgtaa
     2521 tgtgatgtcg caccacgtat tacacaacct aaagtaataa cagcatcata gttccctgaa
     2581 gccgcaagtt tcttagctac taaaggaatt tcaaacgcac ccggaacaaa tgctacatca
     2641 atattgtctt cattaacatc atgtcgaatc aaagtatctt ttgcaccttc aagtaatctt
     2701 ccagtgataa aatcattaaa tcgactaact acgattgcaa ctttcaaatc ttttccaatt
     2761 aatttacctt caaaattcat gttaaagtcc tcctatatta aatgacccat ttttattttt
     2821 ttcgtttcca tataatcatg attatgtacc gtttctggta cgataacttc aattctttct
     2881 gcaatatcaa tgccatattg ttttaatccc tcaaatttac ttggattatt acttaataaa
     2941 ttgatatgtt cgatgttaaa atattttaaa atctgtgcag caatatgata atctcgcaaa
     3001 tcttcatcaa aacctaatgc taaatttgca gttactgtat catatccttg ctcaattaat
     3061 tcatatgcgc gtaatttgtt taacaatcct atgccacgac cttcttgagg tagataaata
     3121 atcatgccgc catgttcatt gatatacttc atagacgatt caagttgagc accacaatca
     3181 caacgttgac tatggaaaat atcgcctgta aggcacgcag aatgtaagcg tacattttca
     3241 tgttgtcgaa ttgcaccttt tgtcagtaca actatctctt catctgtgta agtcgcttta
     3301 aaaccataca tatcaaatat tccgaaatct gtaggcattt tcacttttgc cttaaattca
     3361 atttctggtt ctaatttttt acgatattca attaaatcat caatcgtaat catctttaat
     3421 tgatgttttt ctttaaactt ttgtaaatct tgtcctttcg ccatcgtgcc gtcatcattc
     3481 ataatctcac aaatgacacc agcgggcttg gcaccagtaa gtttagctaa atcgacagcc
     3541 gcttctgtgt gtccatttct agctaacacg cctttatctt gtgctactaa tggaaataaa
     3601 tgaccaggac gattaaaatc tttagcttca ctactaggat caatgagctt tttggcagtc
     3661 aatgtacgtt cataagcgct aattccagtt gttgtatcta catgatcaat actcactgta
     3721 aattgcgtac caaagatgtc ggagttatca ttaaccattt gtaccaaatc caaacgttgt
     3781 gcaatatctt tagacactgg tgcgcatatt aatccccttg cttctttcgc cataaaatta
     3841 atggtattat cgttcatcca ttcagtgacc gctactaaat caccttcatt ttcacgattc
     3901 tcatcatcta ctacaataat tgtttctcca ttttttaaag ccattaaagc actgtcaata
     3961 ttatcgaatt gcatgctacc cctcctaaaa accaaatgct cttaatttat ctacagataa
     4021 ttggtcttta tctttattta aaatattttc aacatattta aacaaaacgt ctgtttctaa
     4081 atgtacttta tctcctaatt ttttggatga taaaatcgtt gaacgcctcg tttctggaat
     4141 aagatgaatg tcaaaactgt tatcatcctt atcaaatacc gttagactta caccatccac
     4201 agtaatagac ccttgcttaa ctaactgatt attaatatgt tggctacatt gaatcgtaat
     4261 aattttggca ttggctgttt catttatttt tgaaactgtt cctagttcat ctacatgacc
     4321 gaggacaaaa tgtccaccaa acctaccgtt accactcatg gcacgctcta aatttacttc
     4381 tgattgtcgc ttaacatctg ctaaataggt tttattttca gtgcctttaa ttacttgaac
     4441 agtaaaagat gtctgatcaa aatcaatcac tgttaaacat gcaccattaa cactgatgga
     4501 atcaccaata tgcatatctg ccgtaatctt atgtgcttcg atttcaatcg tcctgactga
     4561 ttgacgaatt tgaacacttt taacgacacc tatttcttca acaatgccag taaacatgca
     4621 tcatcacttc tttcgtaaag ttaatttaac attttgattt aataactcgg aatgaacaat
     4681 ttcaaattgg ttcgcatctg gtatctcaat cacatcattt gtttgataaa attgataatt
     4741 tccagatcca ccaattaatt tcggggcata atagagaata aattcatcta tataattaga
     4801 ttggagaaat tctgaagtag tggttggacc tgcctcgact agcaaagttc caactcctct
     4861 tttatataaa ttgtgaagaa ttgttgttaa atcacaagac ttcaagtaaa taatttcaat
     4921 atgtgtttga ttggttgtta aatttggatt ttcagtatat atccaaatta gtgttgattc
     4981 atcttgataa atttgctgat taaaatgaat attcccagac ttagacaata ttacttttat
     5041 agggtttttt ccatcttgaa tacgtgtagt atattgtgga tcatctaatt caactgtacg
     5101 tcttccagtt aacactgcgt cgtgtcgatg tcttaactta taaacatctt gtttaacctc
     5161 tttattagta atccattgac tttgtccatt atcattcgct tgtttaccat ctaaacttgc
     5221 agatactttc actgtaattt gtggcagttg ctttgctttt gctttaaaaa agtcttggta
     5281 taattgtgat gcacgttcat catcaacgca ttcaacctca ataccgtgag cccgtaacgt
     5341 atcatcacca tgtgtatcta acgaattgtc ttttgttgcg taaactactt tagctatctt
     5401 acaatcaatt attttgttaa cacagggtgg tgttgaacca aaatgactac atggctctaa
     5461 cgtaatataa atcgtcgcac cttcagcatt ttgttgtgcc atatcaagtg cttgaacctc
     5521 cgcatgcttg ttaccttttc tcaagtgtgc accaataccg acaatcctac cttctttaac
     5581 tacaactgcg ccaacgggtg gattaacacc tgtctgacct tgtaccatat ttgcaagttg
     5641 aatcgcataa tccataaatt gactcaaatg atcacctcta taaacaaaaa tcctcacatc
     5701 atgaattaag atgcaaggag aaaaatttat cgttaaataa gcctatttgt acacattttt
     5761 acaaatacgc tacattatct ttgtcgataa ttaacattct ttctcccatc cagactttaa
     5821 ctgtcggctc tagaatctca ctagatcagc cactaatatg aaacatatta gcaggtcgca
     5881 ggcttaattt actgccggtt gggaatttca ccctgccccg aaagaattat atatgaaatt
     5941 gttatagatt atttgagtac gtagtatgtc aactacattt aaaatgatac tatatgtttt
     6001 ctgaaaaaac aattaatgac ggttttaatt taatataatc tgagtactat aggcatctca
     6061 ttgatatgat tcttactaac agacattaaa atcaaacctt caattcgtct ctatagagcg
     6121 ttctctttat tatcttctag ttacaaatta ttgattgtca ctgcgctgtt gttgctcatt
     6181 cgattctaaa gcatcatata attgagatac tgtatgcgca acttgttcta ctatcatttt
     6241 cacaccattt cgtagtttat taacaccgtt tgtcatttga cctatcgcaa tcatatttgt
     6301 taatgttcca aaccttggac taataacttg attggtttca ggtatgattt gtatgcctcc
     6361 cattggatgt gcttgtacaa tttgtctatt ttcaagattt ttaattaatt gatcatcttg
     6421 atccaattca tttaaatgac tttttgcacc tgtcgcgtta atgacaacat tatatatgtc
     6481 tactgattct tggtttttgt atgaaaaata atacaacttg ccatcatgtt tcacatcttc
     6541 taaatctttt ttcaaaatta aagacttatt ttctattaat tcaataatta gttcagcagt
     6601 tcttggaggc attggatttg aatttaattg aatcatcttt gagtattttt gattaaattg
     6661 atgttggtct tcaatactta agctattcca tatccaattt aaattctctt tcaaatgttc
     6721 aatcatactt tggaaaatgc ccatttctgt tggacgcgct aaatcatact tcaaatctgc
     6781 aatatgattt cctgtacgtc tatgtactaa ttttttaaaa tcaatgtcat attcagcaca
     6841 ttcttttaaa aataaagaaa ctaaagtatc aagcggtgca ttgccgaaat gatgtttttt
     6901 aatgtcattt aatttgtctt tagttaagta cttgaatgtc acgtctatca ttgtacctct
     6961 tacacttggt aaatgagcag aacgactcgt catagtaatt ggtaatttcg gatgatgagc
     7021 agcaacataa cgaacaacat ctaaactggc aaggcctgta ccaataatcg caatatcgtc
     7081 cagttcattt acttcgtcta acgtattata tgttggataa ggcgtagcga tatatccttt
     7141 tttacccttt aagttatatg gatcatggta ggcaaatgta ccacatgtta aaaatacata
     7201 atcgtacgct tgccatgatt gttctgaatt tgtagtacat atgtaataag ttaaattcgt
     7261 ttcatcgata ttagaatttg tataaatctc tcgaacttta ttgtaattag ttgatatatt
     7321 tggatatttt ttcgtgaaca tagataaata agattgcata taatgtccga atacaaatct
     7381 cggtaaatat gcaggttcat caaaattaaa atcagtttgt tgtttatacc acttccaaaa
     7441 ttcagtctca tcatctaaat ttaaactcat ctttttcgaa ggcatattaa ttagcagctc
     7501 agaactatca ttttgaaatg gtacgccctg tcccatattt actttatcat cgtataaatc
     7561 tatatctaat tgattaaact tcgggtgctt aactaactct ctcaatacac ttacaccagc
     7621 agttcccatg cctattattg ctacacgcat atatcatcca tccttttgta attcaaaata
     7681 ttatatttaa actattacct aaaattatag caatttggta tttttaataa aatttattaa
     7741 ggtaagctat tatttcactt tcacaaccat taaaccattt aaaaattttc attta
//
LOCUS       DAHHRR010000109         7665 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.109, whole genome shotgun sequence.
ACCESSION   DAHHRR010000109 DAHHRR010000000
VERSION     DAHHRR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7665
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.109"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(340..699)
                     /locus_tag="NBI42_002130"
     CDS             complement(340..699)
                     /locus_tag="NBI42_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HCD1778525.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(712..948)
                     /locus_tag="NBI42_002131"
     CDS             complement(712..948)
                     /locus_tag="NBI42_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HCD1778526.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            1265..2329
                     /locus_tag="NBI42_002132"
     CDS             1265..2329
                     /locus_tag="NBI42_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1778527.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(2389..2712)
                     /locus_tag="NBI42_002133"
     CDS             complement(2389..2712)
                     /locus_tag="NBI42_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HCD1778528.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            2813..3055
                     /locus_tag="NBI42_002134"
     CDS             2813..3055
                     /locus_tag="NBI42_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCD1778529.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(3318..4493)
                     /gene="dltD"
                     /locus_tag="NBI42_002135"
     CDS             complement(3318..4493)
                     /gene="dltD"
                     /locus_tag="NBI42_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCD1778530.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(4490..4726)
                     /gene="dltC"
                     /locus_tag="NBI42_002136"
     CDS             complement(4490..4726)
                     /gene="dltC"
                     /locus_tag="NBI42_002136"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HCD1778531.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(4744..5958)
                     /gene="dltB"
                     /locus_tag="NBI42_002137"
     CDS             complement(4744..5958)
                     /gene="dltB"
                     /locus_tag="NBI42_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HCD1778532.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(5955..7412)
                     /gene="dltA"
                     /locus_tag="NBI42_002138"
     CDS             complement(5955..7412)
                     /gene="dltA"
                     /locus_tag="NBI42_002138"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCD1778533.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLAGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVSR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(7428..7580)
                     /locus_tag="NBI42_002139"
     CDS             complement(7428..7580)
                     /locus_tag="NBI42_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCD1778534.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
BASE COUNT         2501 a         1312 c         1148 g         2704 t
ORIGIN      
        1 taagtctttt aaattatgta aaaacctgtg atgttcacaa aaaagacatt tttaatccaa
       61 tactaattct atagctttta tgttatattt tcaagcaaga ttcagccaga ttgtgaaaaa
      121 attaattaat tttttcgtag cctgatttcg attaaaaatg tatccgttat cacgatcatt
      181 taaaatacta ttaatagtca gattcttaat ttttaataag ctttttaata aaacaaaata
      241 tctttacgca aaaataggac ataatcaaat atgtccctaa tcgtaattga tatcaatttg
      301 tatccccaaa attgggttaa tcattaaaaa gttattttat taacagtttt caggattacc
      361 tgcaactttt gcagttctaa atgaactacc acagccacat gatgcaattg cattaggatt
      421 gtcgatttgg aaaccgccac ccattaatga ttgcttaaaa tcaatagtcg taccatttaa
      481 tacgggtgca tcttttttgt ctactaaaac ttttaatcca aagtattcta agacttcatc
      541 attttcacca ggcgcttctt ctgcacccat accgtatgtt aaaccagtgc acccgccacc
      601 attcacttta atttttaaat agccatctgg catttcattt gctttaagca tatcttttac
      661 ttcgtaggca gctgcttctg ttaatataac cgttggcatg ataactcctc cttaaaaaat
      721 ccaagtttct tttatatgtg catatatatt ttgtaataat tcttccggcg aatcaccttc
      781 aacaatatca ccatttacta aagcatacaa cccggctgaa catataccac aatgtgtcag
      841 gcaaccatac tctaacacat cgacatctgg gtcattttcc agttgattaa aaacataatc
      901 tccacctttt gccatgttag agagacaaaa ttctacgatc ggattcatac ttcaccttct
      961 tatttcattt gttacaatat tatagcattt taaaactggt attttaacat gatgtgctca
     1021 attagcaaca actgatgttt cttaccccag ttatgtaata gtgccttagt tagtacaaat
     1081 atttatttgt agtcattttc aaaaaaatat ataataagca tataaataca tatttacttt
     1141 tttaggggtt attgatgaat caaagacact tgcattttat aactgttcat taacaaatat
     1201 atttatgcac tttgatcttt tcaaaattaa agtaaatatt aacatataaa aggggtactt
     1261 cattatgaaa aacttagttt tgttaggcgg cggatatggt aatatgcgta tcatgtcacg
     1321 cattttaact acatctttac cacaagatta tacagtcaca ttagttgatc gtatgccatt
     1381 tcatggattg aaaccagaat tttatgcttt agctgcgggc acaaaatcag ataaagatgt
     1441 tcgcatgaaa ttccctaatc atccacaagt gaatacagtt tatggtgaaa ttaacgacat
     1501 agatttagat gctcaaattg tctcagtcgg taattctaaa attgattatg atgagctaat
     1561 cattggttta ggatgtgaag ataaatatca taacgttcca ggagccgaag aatatacaca
     1621 tagtattcaa acgctctcaa aggctcggga tactttccat agtattagtg aactaccaga
     1681 aggtgctaaa gttggtatcg ttggtgctgg attaagcggc atagaacttg ccagcgaatt
     1741 aagagaaagt agatcagact tggaaatata tctttatgac cgtgggccac gaattttaag
     1801 aaattttcca gaaaaattaa gtaagtatgt tgcgaaatgg ttcgccaaaa ataatgttac
     1861 cgttgttcca aattcaaata ttaataaagt tgaacctggt aaaatatata actgtgatga
     1921 acctaaagat attgatttag ttgtatggac agcaggaatt caacctgttg aagttgttcg
     1981 taacttgccg attgatataa atagtaatgg acgcgtgata gttaaccagt atcatcaagt
     2041 accaacatat cgtaacgtct atgtagttgg tgattgtgct gatttaccac atgcgccaag
     2101 tgctcagtta gccgaagttc aaggtgatca aattgccgat gtgcttaaaa agcaatggct
     2161 aaatgaacca ttacctgaca aaatgccgga actaaaggta caaggtatcg ttgggtcatt
     2221 aggagataaa caaggttttg catatatcat ggatcgcact gtaacaggac gactcgcttc
     2281 tattttaaaa tccggcgtac tttggttata taaatatcat aatggttaaa aattaaaaag
     2341 caatgtcagt tcacactatt actgacattg ctttttatta tgtgtatatt attcattcac
     2401 aagcttttgg tctataaatc gagtgatttg ctttgtttgt atataaccat ctgctacata
     2461 ttcatcgttc attgtaatta atggataaaa tagttcatct tgttctattc tttcaataaa
     2521 ttgtaaatca tgatctgtta agttgtcatt atcttttgta atatctatat acgtatattt
     2581 aaatgatata tttgggtatt ttctttttaa tagcggctgt agccagtcat aaatatcttt
     2641 cgatgttggc gcattaacgc aacttgcaca tataacatct gccccatata ctaccacact
     2701 cacgtgctcc atatatttat cccccattgg tttgatagat ttttattaca ctatctatta
     2761 taatatacta ataaagatta tcaaattcat ctctcgaaag gagacttgcc tgatgcctac
     2821 tgaagataca acgatgtttg atcaagtagc agaagttatt gaacgtcttc gcccattttt
     2881 attacgtgat ggtggcgact gctcattgat tgacgtggaa gacggtattg ttaaattaca
     2941 attacatggt gcatgtggta catgcccaag ttctacaatc actcttaaag ctggtattga
     3001 gcgtgcatta cacgaagaag tgcctggtgt aatagaagta gaacaagtat tctaatataa
     3061 agatattaag ttaacagcac atattatgaa taataacaaa gacaggaata ctgaattatt
     3121 tataatcagt tattacctgt cttttttcgc tgtcaaattg gactgatggg aaagttattt
     3181 tatactattt atctgattaa ttatttcatt atagccacat atccaagcgc atattactat
     3241 tctattctat gaaatttaaa atataaaaat agcacatacg ctcaatcaaa atcgttgagt
     3301 tatgtgctat ttgtatttta atttttaggt ttatctactt caggttgtgg ttcacctttc
     3361 atatgtttcg caatttgctc atccatataa acccaacctt tccaaccgat gtgtacggca
     3421 tcactgataa catatttttc ataatcttta tcagtcatat cgtaaatttt accaccatta
     3481 tctacaacag tagaatggat ttttttatac actgcttgac gacgttcttt atcgatgcca
     3541 atgtggtcat accatacacc gtttgatgga atacttacat attgaacatc tgcaccagca
     3601 gcacgcattg tttttacaag taattctaaa tcttggaatt ctggagaatt aacattgaat
     3661 tcgtagtcac gtctaacttt acgcttactt tcttgaatta atttccagta ttgatctcta
     3721 ataccaaatt tattcgatgt agtatcagct ttaccaattt ctactgcttt ttgtttcatc
     3781 tcatcccaag aagcgtctgg ttttgtagca ggtttaacat gttctaatgg agatttatcc
     3841 attgcaaaca atgatttaat cgcttctatt ttaatcaatt gattctcttt aaaaccagaa
     3901 atataactat ctttagtttc tttagggttt ttagcataag atttcaagta ttctttattg
     3961 tgtacatgtg gaaactgtaa taaacgttgt gcataacgac gttttaattc agtagacatg
     4021 tttttctgct ggaacatttg attaatttga gtttgagaca tacgagcatc aaagttttga
     4081 ttcgttaaac catggtttgt aaaccattgt ggtgaaataa taaatgttaa tttcttacct
     4141 tttaatttat catactgtga tgcaagttca actgcgttaa ttaagtctgt agaaccacca
     4201 gcacctaata agaatgcttt tttgttggcg ttatgcttgt ttaatgcaat tgcaggatta
     4261 aatgggtcat ctttacctaa ttcactagag ccgtatatag gataatactt atttgattcg
     4321 tataacttat cttgaatgag tgtgcctttt agtacttgat ctgtcaatga agttctatta
     4381 tcttctacag tcttttcatt tactaatcct gtaaaccaac tagcaggtaa taatagaaag
     4441 acaatgaata ccgctccact aattaaaatg ggtaaaaaag gttttaattt catcgtaact
     4501 cttctaatgc ttcaacgatt ttatttggtg ttgcccactc atctctatca aagtccataa
     4561 tagatacttc gatatcaagt ttattttgaa tttctaataa taatccaact gtttggaaag
     4621 aatcaataat accttcttca aaaatttcta cgtctggatt ttcttttaca atatcatttt
     4681 ctgctacttc tgctaataaa tttaatactt gttctctaaa ttccataatt aaattctcct
     4741 ttattatata agtttacctg agaagattaa aaagccaaat gttacaaagt ggaatgtaat
     4801 cacaatgcta agtgctgttg tgaaaccatt ttgccaacgt ggcggatgtt tcttgcgcca
     4861 acgttcataa tagccataac ctataaacaa tgctgcatgg tataaaccat aaacaatgta
     4921 atacacttcg ataccatgcc aaattcccat tatgaagaag ttgattaaga atgccacgtt
     4981 agacattgca aattgactct tcaataattt tttacgagac atgtagaata aagatctcat
     5041 gtaaatacaa tctctgaacc agaatgataa tgtcatatgc catctattcc agaaatcttt
     5101 aatatttttc gctttgaaag gtttatcgaa gtttggtggt gttttaatac cgaataaata
     5161 actaaatgct atcgcaaata aactataacc tgcaaagtca aagaataaat ataagctgta
     5221 tgcgtacata tataaccaca aattgacaaa gccatgtaag tctaattgta acggcatgat
     5281 tgcatatgtg ttaataaagt aagcaacaat atatttatac aagaaaccaa gcataatcat
     5341 gtgaattgct tttaatacta attcacgata ttcattgcct gttggtactt ttttatcgtc
     5401 tttaacgaaa cgtttgtaac gatcgattgg tccagatgaa atcgttggga agaatgaaat
     5461 aaattgaatt aatttccata ctttaatttc tttgatagaa ccatcacgaa tttccataat
     5521 taactgcaca cttttgaatg taacataaga aattcctaag aaaccaacaa attcaattaa
     5581 tttactttca tggaagtgaa tctgatgtcc acctaaccat gtactttgta acactttcac
     5641 aagtgctaat ggcaatattg ataaaaccat tacagttaca aattttgaaa atgaattatt
     5701 ttttggtttt gaatgataat aaaacattat caataaaact tgccatacga cgtaaataat
     5761 aaaactaatt aattgaacac ttaaatactt ttggtcaaac agattatgtt tatcagaaga
     5821 aaagattaac acaatcataa atgctgtaac tacgccatta taaatgtaac ttcgcttacc
     5881 taaaaatcca agtataatga ctggtaataa tgcaattaaa gcaattaaga agaatgtaaa
     5941 atcaccatat ggaatcatcc gttaattacc tctgcaattt tctttctgtc aattttacca
     6001 tttgaagtca atggcaattg ttccatccat tcaaactttc taggaatcat atactctggt
     6061 aagcgtgatt tcaagtcatt tttaatattt ttagtcattt ctgcattgtc cgtaacttca
     6121 gtcgttggca caattgcacc aattaaatga ataactttat catttttata tacaggtaca
     6181 acaatcgctt cttttacgaa ctcagactgg cgtaattgtg tttcaatttc ttctaattcc
     6241 attctgtagc cattcaattt gatttggaaa tcaatacgac cttgaatgaa ccattgacca
     6301 ttttcaaact tcgctttatc accagtgtga taagtacgaa taccgtcatc gaaattaaat
     6361 acttcagctg ttttttggtc attttttaag tatcctaaac ttacactttg accttcgata
     6421 acaagttcac cttcatctgt agtagataat cttgcgcctg gtctttcaac accaacaggt
     6481 aatgtcggat attgatctaa gatttcttgt gtaatttgaa tacttgtaac tgctaccgta
     6541 gcttcagttg gaccatatgt gttgtaaatc gtcgcacttg ggaaacggct tactaacgct
     6601 tttgctgctc tgtgaggtag aatttcacca cagaagaaga attcgttaag actaccatac
     6661 tgttcttcat taagcgttgg taataataaa cacatttcca taaatgatgg tgttgatacc
     6721 caaatgttaa tcggtgttgc tgttagcatt tcatttaata gtttaggttt attaatcatg
     6781 tttttatcta caagatttaa tgtaccgcct gacgctaaac atggataaat agccattaca
     6841 gataaatcaa atgaaaatgg cgcttggtta agccattgtt gttcatttcc tgatttatta
     6901 agttctaaca tccactcagt aaattgaact aaacttgcat attcaatttg aacaccttta
     6961 ggttccccag tagaaccaga tgtaaagatt gtgtatactg tgtcgttatc tttaatctga
     7021 ctatcaaaaa ttactgggtc ttgagatgtt ttaatatctt ctattgtaaa tacttcgcct
     7081 gctaaacttt caaatgattc atcagtcgta ttaaatacaa actctggttg aaccttgtta
     7141 ataatcattt taatacggtc ttcaggaatt gaagtgtcta caggtacata tccacatcct
     7201 gctttaatgg caccaatcat cccaacaatc atatatggtg acatgtgacc gaataaaatc
     7261 atcggtttct tactaccttg taatcgatgt gctaatttac tagactcatc cattaactgt
     7321 tgataagtta attcatcagt tgtgtgtcta acagcaatgc tttgtggatt tgcatccgca
     7381 aacgcttgca gcttgttaat aatatctgtc atattaagtc tccctcatta gaactcatta
     7441 taaatgaagt tattgtgtgt gtcgccactg ccataaatta aatataaagt aataaatatt
     7501 gccaaataca atagtgttaa taaatatggt ttgaatgctt caacatattt attaggtggc
     7561 tgtttacttt tagatttcat attgcacctc ttaaagttct tagtaaaaac gcctttataa
     7621 agaccgttca atataaaata cgttttaaaa tttgtttttt acaat
//
LOCUS       DAHHRR010000110         7616 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.110, whole genome shotgun sequence.
ACCESSION   DAHHRR010000110 DAHHRR010000000
VERSION     DAHHRR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.110"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(132..824)
                     /locus_tag="NBI42_002140"
     CDS             complement(132..824)
                     /locus_tag="NBI42_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCD1778535.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFAGVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKR"
     gene            1135..2079
                     /locus_tag="NBI42_002141"
                     /pseudo
     CDS             1135..2079
                     /locus_tag="NBI42_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2066..2368
                     /locus_tag="NBI42_002142"
     CDS             2066..2368
                     /locus_tag="NBI42_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778536.1"
                     /translation="MRTNNYITDLDAIKLSSAWNYNPKLVTGKNALLSDSLLRDIKVL
                     SSNAKGDADMLETELRAVINAMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            2522..2881
                     /locus_tag="NBI42_002143"
     CDS             2522..2881
                     /locus_tag="NBI42_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778537.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVATYKHFEAGQGKKGAQHALDL
                     LGLATLSLLDTFSCNKK"
     gene            complement(2966..3577)
                     /locus_tag="NBI42_002144"
     CDS             complement(2966..3577)
                     /locus_tag="NBI42_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCD1778538.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTKLSLLASTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(3656..4132)
                     /locus_tag="NBI42_002145"
     CDS             complement(3656..4132)
                     /locus_tag="NBI42_002145"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HCD1778539.1"
                     /translation="MAVVYVAGGCLWGVEAFFITMPGILHTEAGRANGRSSKLDSPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGEKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(4135..4635)
                     /locus_tag="NBI42_002146"
     CDS             complement(4135..4635)
                     /locus_tag="NBI42_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1778540.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATPEALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVPTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFENLAKNQHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDDV"
     gene            complement(4870..5637)
                     /locus_tag="NBI42_002147"
     CDS             complement(4870..5637)
                     /locus_tag="NBI42_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCD1778541.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILQGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(5634..6326)
                     /locus_tag="NBI42_002148"
     CDS             complement(5634..6326)
                     /locus_tag="NBI42_002148"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCD1778542.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIEDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNKDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(6343..7005)
                     /locus_tag="NBI42_002149"
     CDS             complement(6343..7005)
                     /locus_tag="NBI42_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HCD1778543.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNRISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         2526 a         1363 c         1073 g         2654 t
ORIGIN      
        1 attcttcttt aaaaatataa ataccacgac atattgcttt aacattttca tttataaagc
       61 gaaaaaatgc atcgctacta agttgaatat ttagtaacga tgcattgaat tcactaaaat
      121 gattaaatta cttatctctt ttcatctgat tgattatcga aatttcttcc ctctaaacct
      181 gctaactctt ctttagaagc tgcaggtgct ttcatttcaa atatctcatt cactactgtg
      241 taatcgtaat atcctaatct ggcaataggt ttaatcgact taatgtccaa tttaccatta
      301 tcaagaataa ccttatcgtc aatatgaact tgggcaactc ttcctataac aatatctacg
      361 gtagatactg gatctccagt tggaatacga atcgtttgaa cgtactcaca ttcaaaatgt
      421 actggcgatt ctttgactcg ataacctggt gcttctatac atttttcctt tgttacacct
      481 gcaaaattaa attcatcctc ttctggtggc aatgctttcg atgataaatt aactgcttct
      541 cttaaatcat acgttgccat attccacaca aaccaacctg tctcttcagc atttttcact
      601 gtatctttac gttcgtgatc accaagaacg gattgatttg ctgcgaacat aaccataggc
      661 ggatcccaag ttaagttttg atactgacta taaggtgcta aattatcttt cccatctttc
      721 gatacagtag atatccaccc tattggacgt ggtactgtac tacttttaaa tggatcgtgt
      781 ggtaagccat gactccttac accttgcttc ggcgaataat tcattctatc ctcacccctt
      841 attagtaatt acatttaagg ttacgctctc atgacataag cgtctaatat aaataaacaa
      901 tttatttata agtagaaact atatatgatg tggttgcttc tcttgtatgc ttacttctca
      961 atttatagaa atatgtttat gcgattaata tttatgatat cttttgtaaa tttaattaat
     1021 tatagttact tcaatcatga ttagtttata ataatattga ataacaatat tttgattttt
     1081 gtataattta tctagattct gaaaaatagc gcaatacaaa ggggttatct atctatgaga
     1141 aaacttatgc aaatcatgtt atgtgcatca atcatattaa gcggttgctc atttatgcat
     1201 aaagatcaaa caaaagatat tccaaaaacg gttagcgtca aagattacga tggtaaatat
     1261 attggtgaac ataaaaaaag aaatgaagta tttctgaaaa aacataaaga tgaagcaatc
     1321 aaaaaatata aagcctatgt taaagatact tttggttatg attgtaaaat taatttaatt
     1381 gatgcctata ctaataaatc tggattcagc gagaaaagta aaacggatgg attagtcatt
     1441 gttggtactg tcaattacga tatccctttt caatttagac tgatttttgt tgaatctaac
     1501 aatggcatag ctatcacaac atttacacct ggccataaaa acgaaacatc tgcagctgtt
     1561 gccgctatga tgtataagcg ttatgaacat gaaattgaac aagctcgatt aaaattcaaa
     1621 tcagaagttc agaaaaatgg ttattatgcc atgaatgaaa aacttcaagt aaaacaagaa
     1681 tttaacggcg ttacaaggca atacttaaat ttcagaacag atagtatcga tgatctggat
     1741 aaattcaaaa aagaattcaa acctgttatg cacttaaaag gtgatgcatt caatcaacag
     1801 ttacaatctt tgattaacaa atatccacaa atacaaaaaa atatgaaatc agagttcatt
     1861 gcttattatg ataaagaaaa aaatagagaa acagtaaaaa actatgctta gaaccttcaa
     1921 aaatctataa atgacgttat gcaatcatat cctagcacaa aatttgtaca gttttataaa
     1981 gatgatgttt ccccgtcaat agtagatgga aatggccgtt taaaatcgga tgctaatgta
     2041 atttccatcg agggaggaaa gtataatgag aacaaataat tatataaccg atttagatgc
     2101 tattaagtta agcagtgctt ggaattacaa tccaaaattg gtgactggca aaaatgcact
     2161 actatcagat agccttttaa gagatattaa agtattaagt tctaatgcta aaggcgacgc
     2221 tgacatgcta gaaacagaac ttcgtgctgt aataaacgca atgtctcaaa acgaaggtaa
     2281 atctgttgat gcacttaaaa atagtaccgc ttctctaaaa aacaatctaa ccaaattaaa
     2341 agagcaactc gacaaaacta cacattaatt tcatacattt tgagtgaaca gaattatatt
     2401 tccatagcaa acattcctaa actacacata cgttacataa ttgattcatt tttatagaaa
     2461 cgggtaaaaa tgataaagta attatctttc acaaaaatcc aaagattggg aggaattaaa
     2521 tatgattttg cgttacgcaa cgaatttaaa acttgcgaaa gagctatacc aagccagtaa
     2581 acctaaattg caagaagata agggtatgat tgaactgttt gaacatactt ttggcttaca
     2641 aaaagaactt gtaaaatacg tcggcattgc agaagcaact actgctgctt tatactcagc
     2701 tagctttatc aataaaaaca tttctcgcct tgcatcacta tcaacgatag gcattttaag
     2761 tgtagctact tacaaacatt tcgaagctgg ccaaggtaaa aaaggtgcac aacatgcatt
     2821 agatttatta ggtcttgcta ctttaagttt actagataca ttttcttgta ataaaaaata
     2881 gtcttcattt tagaagcaca tactttagca catatgtact ttggatttaa tccataaaca
     2941 gtacactgct atagcatgtg ttttattatt tttctggtct ctttatcgta aatattcccc
     3001 ctaatttagc ataatcattt ccagctaaac gagaaacagc ctgtaatgcg tgtggattga
     3061 ttttaaaatc accctcataa atactgtcat caatatgata agttacaatt tcacctatta
     3121 ttaaatcagc cccatctaat acatctccaa gcaatatcat ttgcgatagt ttacattcga
     3181 atctcatttt cgcgtcttta attcctggcg tcttaatcgt tgtagatgct aaaagtgata
     3241 attttgtacg gctcaactca ctgtcaccat atgctaacgg cgctgcagtc tcattaatat
     3301 cttgaacatt atcttcgtct gtaatatgca caacaaagtc tccggtccgc tctatattca
     3361 aggcagtatc ttttctctta cctcctgcac gttgaactgc aatagcaatc attggcggat
     3421 ggttattaac aatattaaaa aagctaaatg gtgctgcatt tactgatgca tcttgattta
     3481 atgttgtaac aaaagctata ggtcgtggaa taatcgaacc aattaataat ttatagtttt
     3541 ctctagcagt taatgattgt gcatcaaacg tatacataat acctacctct tttcttagta
     3601 tatctaggta tttctccgat tttggttaat ttaaacatct attctcctct gaaaatcact
     3661 tgtatttatt tagcaaatct tttgaaatat gacacatatg catatcttct ggatattttt
     3721 ctaaatgttg ctgatgttct tcagcacttt taatgtagtt agacagcggt aagacttcca
     3781 ctgcaatttg atctctgtct ttacgtcgtt caatgaactg acgcgcttca attaagtggt
     3841 catctacaca actatataag cccgttcgat acttttctcc aatatcattt ccttgttgat
     3901 tcacactgta aggatcaatg atttcaaata aataattcat aatgtctgta attgttaaca
     3961 tacgatcatc gaaatgaagt ttgacacatt cagcataacc atcatacgga ctgtctaatt
     4021 tagagcttct tccatttgct cttcctgctt ctgtatgtaa aattccaggc attgttatga
     4081 aaaatgcttc aacaccccat aaacaccctc ctgctacata aacaactgcc atatttacac
     4141 gtcatcatcc ttttttatat ttttaacaag gttataccat ttaataccgc catgacatga
     4201 ttctgataca ccttcattac gatacccata tttttcataa aatgaaatta atgattctcg
     4261 acatgttaac gttacaccat gtcgatgttg attcttagca agattttcaa aatagtttag
     4321 taagcgacct gcaataccct gaccttgata atttggtgct acaacaagac ctaacacact
     4381 aatatagcca ccttcactat tatttgtggg gacattttta aataaatcat cgctaatgta
     4441 acgctctttt atgactgggc cgttgatgta tccaattact tcatcattct tttcagctac
     4501 aataaaagtt tcttgaattt gttcaattct caatttcaat gcctctggtg ttgcagcttc
     4561 ttccggtgaa aaaccaatat tttcaagtgc aataatttga tctaagtcct gcatgattac
     4621 ttttcttaat accataaaaa cacttcctta gccctatata tcaaaagtaa taccttcatc
     4681 tttatattca ttattttcaa actaccttca ctataaaaaa cgagtctttt tattgaaagc
     4741 cattcgcctt aatcaattag acaagttgta ttatacgaat tagcaaataa tcatgattat
     4801 aattcatttt aagcatactc aaaaagactg gtacatgtat accagtctaa ttcgaaaaaa
     4861 tatatttagt taaaaccatt tcaaaaacga cttcactttt cctggcttag tcggtctgtc
     4921 taaataaaca aaattatcat tgattactga actggcattt cgaagtaatg cttcaatatc
     4981 attcgaatat ttcttcaatt tatgattgtg aaataattct tgcatcaaaa atggtctttg
     5041 gtcacatgaa tgtgcatctg aagctacaaa atgagccaaa ttacattcta taaattgtaa
     5101 tgataacttt tgaatgtttt taccaaatcc accaactaaa gaactcgatg ttaattgact
     5161 cagtgcccca tttgcaacca attcatataa tatttccgga tttttggcga tacttctatt
     5221 tctttcagga tgtgcaatga ttggtatgta acctctcgat tgtatttcaa aaaacaattg
     5281 ttttgtataa tgtggtactt cgcccgttgg aaattcaatt agtaaatatt tcgaacgatt
     5341 aataccttga atactaccat tatctaagcc ttgcagaatc gaatctgtaa ttctaatttc
     5401 ttgcccggga aataatttaa tatccaatgc ttgaacttct ggatgcgttc ttaactccgc
     5461 caatttcaca agcacttgtt gaaatgtatt atcatatctc ggatgcaaat gatgaggtgt
     5521 cgctacaata cttgttacac cttcatcctt agcttgcttt aatagtgcaa tactcttttc
     5581 aattgtctta ggaccatcat ctatatcaac taatatatgg ttatgaatat caatcatgat
     5641 tcatcagtcc cataatatgc atagtaacta gcacttttat ctttaggcat tctatttaag
     5701 actacaccta ataatttagc acctgttgct tcaataagtt cttttccttt tttaacttca
     5761 tctttattat tattttccga attaactacg tagacaacat tgccggtaaa ctttgaaaat
     5821 aattgcgcat ctgtaactgt gttcactggt ggcgtatcga taattacaaa gttataattc
     5881 atcaataatg tgtcatacaa attcgcaaat gctcttgatg tgattaactc tgatgggttt
     5941 ggcggaatcg gtccagacgt caggacgtct aaatcttcaa tttcagttga gataatactg
     6001 tcttgataag ttgaccaatt tagcaataaa cttgaaagtc cttcattgtt tggcaaatta
     6061 aaaatataat gctgcgtagg tttacgcata tccccgtcta cgattagtgt tttataacct
     6121 gcttgtgcat atgcaactgc taaatttgct gcaattgtag acttacctgc gcctggtgcc
     6181 tctgaagtga ttacaatgct ttgaactgca ctgtcaggat ttgcaaacat aatatttgat
     6241 cttatgcctc gaaatttctc gctaataggt gactttggtt gttcatggac aattaaactt
     6301 gatgtacttc ttcgtgtatt cgtcatggta attcctcgta aattaaaatt tttgtattga
     6361 acctaaaata ggtaatccta gttgcgattc aacatcttct tccgtcttaa tacgcttatc
     6421 taataattct tttaagaaaa taatcaatat tgctaaaaca ataccaacaa taatgctgat
     6481 cactaagttg acagatacta ttggagatac ttttacagca ttatcatgtg ctgaggaaag
     6541 tatcgtaaca ttatcaacac tcataatttt aggcatgtca tgtgcaaaaa ctttagatat
     6601 tctattaaca attttgtcag attcagattt attcccagtt gtaactgata cagtaataat
     6661 ctgagagttt gtttgattgg ttacttttaa aaacgaattc aactcagctg ttgaatactg
     6721 accatcaaac tctctagata ctttatctag aattcttgga cttttgataa tttccgtata
     6781 tgtattaaca gactgcaaac tactttgaac attttggaaa gctaaatcac ttgaggactt
     6841 tttcatgttc actaatattt gagtagaagc agtatatttg tcaggcataa caaaaaaggt
     6901 taatgccgca cttactacaa gacatattgc cggtaaaata agcaataatt taatattctt
     6961 ctttagaata tttaatagtt ttactaaatc aaacttttct ttcatggttt cctccacata
     7021 atcaatcatt gtattcatta cgtatgtttt ataaatcgta caattatatc tagtttaacg
     7081 accacaaaac atccacaact acattttctc taattattta tataaatatt ttatcgttta
     7141 aaattatatc atgattcttg accattatgt ataacttatt tatatttttg tacaatatat
     7201 aatattgtcc gactccaaat atccaaacct attaataata aacctagata ccatcgtact
     7261 ctatcatggc tttcttataa tcgagtagaa gcatcatcat tacttgatta tttgctcttt
     7321 acaacaccga gcgtgcccgt attcggtagt tcaatacatt gcgtaatccg tctctgtgag
     7381 ttgggttaat gataataaag cccacatctt ttaaaaagat gtgggtaatt tatataattt
     7441 ttatttacat tttaaactta tatataaaag cacctatgtc atgatttacc atcacatagg
     7501 cgcttatcaa taatttatta attattactt tccatttcat ctaatttatg cggatttcct
     7561 gtaattagat gacaacttat tcttttcagg ggaacattac atttttataa tatgtt
//
LOCUS       DAHHRR010000111         7606 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.111, whole genome shotgun sequence.
ACCESSION   DAHHRR010000111 DAHHRR010000000
VERSION     DAHHRR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.111"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            30..4163
                     /gene="sdrD"
                     /locus_tag="NBI42_002150"
     CDS             30..4163
                     /gene="sdrD"
                     /locus_tag="NBI42_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HCD1778544.1"
                     /translation="MLNRENKTAMTRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSNSKVDNQQLNKEDNTKNDNQKEMVSSQGSE
                     TTSNGNKSIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQSDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNAETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVKPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLA
                     LKSQITVDDKVKSGDYFTIKYSDTIQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNDTTKTTANIQ
                     YPDYVSRDNNSIGSAFTETVSHAGNAEDPGYYKQTVYVNPSEKSLTNAKLKVEAYHKD
                     YPDNVGQINKDVTKIKIYQAPKDYVLNKGYDVNTNQLIDVTEQFKDKITYGANDSVNV
                     DFGSINNSYVVMVDTKFEYTTSESPTLVQMATLTSDGNRSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPQGYEVTPSKQGNNEELDSNGVSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQGEKGISGVTVTLKDENDKVLKTVTTDADGKYKFTDLENGT
                     YKVEFTTPAGYTPTSTNTGGNDTVDSNGLTTTGVIKDADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPAGYTPTQVGSGNDESVDSNGTSTTGIIKDKDNDTIDSGFYKPKYNLGDYVWEDTN
                     KNGIQDQGEKGISGVTVTLKDENDKVLKTVTTDADGKYKFTDLENGTYKVEFETPAGY
                     TPTTVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGTQ
                     DSTEKGIKDVTVTLQNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYFEEETSDSDSDSDSDSDSDSDSGSDSDS
                     DSDSDSDSDSDSDSDNDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSGSDSDSDSDSDSDSDSDSDSDSDAGK
                     HTPVKPMSVTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK"
     gene            4561..>7606
                     /locus_tag="NBI42_002151"
     CDS             4561..>7606
                     /locus_tag="NBI42_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HCD1778545.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQP
                     EAKEESTTSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKQTVNVSKE
                     DLKNDPEKLKELVRSENNTDHSTKPVATAPTSVAPKRLNAKMRFAVAQPAAVASNNVN
                     DLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSD
                     LTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKARLTLYSYIDKQV
                     VPNETSLNLTFATAGKETSQNVSVDYQDPMVHGDSNIQSIFTKLDENKQTIEQQIYVN
                     PLKKTATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNPNQQLPQSNRIYD
                     FSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMR
                     TTDKYGYYNYAGYSNFIVTSNDSGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSK
                     EKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPAGYLPTKVNGT
                     TDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIK
                     DVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTAEDKD
                     SNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDPTEKGIKDVTVTL
                     QNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTGTNTTEDDKDADGGEV
                     DVTITDHDDFTIDNGYFEEETSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD"
BASE COUNT         3109 a         1154 c         1377 g         1966 t
ORIGIN      
        1 taaaaatttg atgaattagg agtaatctaa tgctaaacag agaaaataaa acggcaatga
       61 caagaaaagg catggtatcc aatcgattaa ataaattttc gattagaaag tacacagtgg
      121 gaacagcatc aattttagta ggtacaacat taatttttgg tctggggaac caagaagcaa
      181 aggctgcaga aagtactaat aaagaattga acgaagcgac aacttcagca agtgataatc
      241 aatcgaatag taaagttgat aaccagcaac taaataaaga agacaatact aaaaatgata
      301 atcaaaaaga aatggtatca tctcaaggta gtgaaacgac ttcaaatggg aataaatcaa
      361 tagaaaaaga aagtgtacaa tctactactg gaaataaagt tgaagtttca actgccaaat
      421 cagatgagca agcttcacca aaatctacga atgaagattt aaacactaaa caaactataa
      481 gtaatcaaga ggcgttacaa tctgatttgc aagagaataa atcagtggta aatgttcaac
      541 caactaatga ggaaaacaaa aaggtagatg ccaaaactga atcaactaca ttaaatgtta
      601 aaagtgatgc tatcaagagt aatgctgaaa ctcttgttga taacaatagt aattcaaata
      661 atgaaaataa tgcagatatc attttgccaa aaagtacagc acctaaacgt ttgaatacaa
      721 gaatgcgtat agcagcagta aagccatcat caacagaggc taaaaatgtt aatgatttaa
      781 tcacatcaaa tacaacatta actgtcgttg atgcagataa aaacaataaa atcgtaccag
      841 cacaagatta tctagcatta aaatcacaaa ttacagttga tgacaaagtt aaatcaggtg
      901 attatttcac aattaaatac tcagatacaa tacaagtata tggattgaat ccggaagata
      961 ttaaaaatat tggtgatatt aaagatccaa ataatggtga aacaattgcg actgcaaaac
     1021 atgatactgc aaataattta attacatata catttacaga ttatgtcgat agatttaatt
     1081 ctgtacaaat gggaattaat tattcgattt atatggatgc agacacaatt cctgttagta
     1141 aaaatgatgt tgagtttaat gtaacgattg ggaatgatac aacaaagact actgcaaaca
     1201 ttcaataccc agattatgtt tctagagata ataattctat aggttcagca tttactgaaa
     1261 ctgtctcaca tgcaggtaat gcagaagacc cgggttacta taaacagact gtttatgtaa
     1321 atccatcgga aaaatcttta acaaatgcta aattaaaagt tgaagcttat cataaggatt
     1381 atcctgacaa tgtaggtcaa ataaataaag atgtaactaa aattaaaatt tatcaagcac
     1441 caaaagacta tgttttaaac aaaggttatg atgttaatac aaatcagcta atagatgtaa
     1501 cagaacaatt taaagataag attacatacg gagctaatga cagtgtaaat gttgattttg
     1561 gtagcattaa taattcttat gtagttatgg tggatacaaa atttgaatat acaactagtg
     1621 aaagtccaac acttgttcaa atggcaactt tgacttctga tggaaacaga tccgtttcta
     1681 ctggtaatgc tttaggattc actaacaacc aaagtggcgg cgctggtcaa gaagtctata
     1741 aaattggtaa ctacgtatgg gaagatacaa ataaaaacgg tgttcaagaa ttaggagaaa
     1801 aaggcgttgg caatgtaact gtaactgtat ttgataataa tacaaataca aaagtagggg
     1861 aagcagttac taaagaagat gggtcatact tgattccaaa cttacctaat ggagattatc
     1921 gtgtagagtt ttcaaattta ccacaaggtt atgaagtaac cccttcaaaa caaggtaata
     1981 acgaagaatt agattcaaac ggtgtatcat cagttattac agttaatgga aaagataact
     2041 tatctgccga tttaggtatc tataaaccta aatacaactt aggcgactat gtatgggaag
     2101 atacaaataa gaacggtatc caagatcaag gtgaaaaagg tatatctggc gtaacggtaa
     2161 cattaaaaga tgaaaatgac aaagtgttaa aaacagtaac aacagatgct gatggcaaat
     2221 ataaatttac tgatttagaa aatggaactt acaaagtgga atttactaca ccagcaggat
     2281 acacaccgac ttctacaaat acaggtggaa atgatactgt agattctaat ggtttaacaa
     2341 caacaggtgt cattaaagac gctgataaca tgacgttaga tagtggattc tacaaaacac
     2401 cgaaatataa tttaggtaat tatgtatggg aagatacaaa taaagatggt aaacaagatt
     2461 caactgaaaa aggaatatcc ggcgtaactg ttacattgaa aaatgaaaac ggtgaagttt
     2521 tacaaactac taaaactgat aaagatggta aatatcaatt tactggatta gaaaatggaa
     2581 catacaaagt tgaatttgaa acaccagcag ggtacacacc aacacaagtt ggatcaggta
     2641 acgatgaaag tgtagattca aatggtacat caactacagg tattattaaa gataaagaca
     2701 acgatacaat tgactctgga ttctataaac ctaaatacaa cttaggcgac tatgtatggg
     2761 aagatacaaa taagaacggt atccaagatc aaggtgaaaa aggtatatct ggcgtaacgg
     2821 taacattaaa agatgaaaac gacaaagtgt taaaaacagt tacaacagat gctgatggca
     2881 aatataaatt tactgattta gaaaatggaa catataaagt tgaattcgaa acgccagcag
     2941 gttatacacc gactactgtt acctctggaa atgatactga aaaggattct aatggtttaa
     3001 ctacaacagg tgtcattaaa gatgcagata acatgacttt agacagtggt ttctataaaa
     3061 caccaaaata cagtttaggt gattatgttt ggtacgacag taataaagat ggtacacaag
     3121 attcaactga aaaaggtatc aaagatgtga cagttacatt gcaaaacgaa aaaggcgaag
     3181 taattggaac aactaaaaca gatgaaaatg gtaaatatcg tttcgataat ttagatagcg
     3241 gtaaatacaa agttattttt gaaaagcctg ctggcttaac gcaaacaggt acaaatacaa
     3301 ctgaagatga taaagatgca gatggtggcg aagttgacgt aacaattacg gatcatgatg
     3361 atttcacact tgataacgga tactttgaag aagaaacatc agacagtgac tcagattcag
     3421 atagcgactc agattcagat agcgattcag gctcagatag cgactcagat tcggatagtg
     3481 actcagattc agatagtgac tcagattcag ataacgactc agattcagat agtgattcag
     3541 attcagacag cgattcagac tcagacagcg attcggattc agatagtgat tcagattcag
     3601 acagcgactc agattcagat agtgattcgg actcagacag cgactcagac tcggacagcg
     3661 actcagattc agatagcgat tcagactcag acagcgactc agattcagat agcgattcag
     3721 actcagacag cgactcagat tcagatagcg actcagactc agacagcgac tcagactcag
     3781 acagtgactc agattcagac agcgactcag actcagatag tgactcagac tcagatagcg
     3841 attcagattc agacagtgac tcagactcag atagtgactc agattcagat agcgattcag
     3901 actcggatag tggttcagat tcagatagtg actcagactc agatagtgat tcagactcag
     3961 acagtgactc agattcagat gcaggaaaac atacacctgt taaaccaatg agtgttacta
     4021 aagaccatca caataaagcc aaagcattac cagaaacagg tagtgaaaat aacggctcaa
     4081 ataacgcaac gttatttggc ggattattcg cagcattagg atcattattg ttattcggtc
     4141 gtcgtaaaaa acaaaataaa taatattatt aacttaacca ggtccgtgag gcctggtttt
     4201 tttatttcaa aatttatgaa atgtatctta tgtttgagat taaatagtgc gatgttttaa
     4261 aaaatataac ataaatcaac ataaatttaa ttcatcttta cattaaaagt acaaacgaat
     4321 aagaaaagca attaaacaca gtatcaaacc atttaaaaca attgatataa aaaacaaacg
     4381 aataattaaa cattactaga attaatttat tcaaaccaaa gtgtaaaata acggtcttag
     4441 ataaataaat ttatttaaag tattgtgctt tatctaaaaa tgtattacga tgggaataca
     4501 aatttttata tgtaaaaata ttaatttttt gcaaatattg atattttaaa ggagatttat
     4561 atgattaaca gggataacaa aaaggcaata acaaaaaagg gtatgatttc gaatcgctta
     4621 aacaaattct cgattagaaa gtatactgta ggaactgcat cgattttagt aggtacgaca
     4681 ttgatttttg gtctagggaa ccaagaagct aaagctgctg aaaacactag tacagaaaat
     4741 gcaaaacaag atgatgcaac gactagtgat aataaagaag tagtgtcaga aactgaaaat
     4801 aattcgacaa cagaaaataa ttcaacaaat ccaattaaga aggaaacaaa tactgattca
     4861 caaccagaag ctaaagaaga atcaactaca tcaagtactc aacaacagca aaataacgtt
     4921 acagctacaa ctgaaactaa gcctcaaaac attgaaaaag aaaatgttaa accttcaact
     4981 gataaaactg cgacagaaga tacatctgtt attttagaag agaagaaagc accaaataat
     5041 acaaataacg atgtaactac aaaaccatct acaagtgaaa ttcaaacaaa accaactaca
     5101 cctcaagaat ctacaaatat tgaaaattca caaccgcaac caacaccttc aaaagtagac
     5161 aatcaagtta cagatgcaac taatccaaaa caaacggtaa atgtatcaaa agaagatctt
     5221 aaaaatgatc ctgaaaaatt aaaagaatta gttagaagtg aaaataacac tgatcattca
     5281 actaaaccag ttgctacagc accaacaagt gttgcgccaa aacgattaaa tgcaaaaatg
     5341 cgctttgcag ttgcacaacc agcagcagtt gcttcaaaca atgttaatga tttaattaaa
     5401 gtgacgaagc aaacgatcaa agttggcgat ggtaaagata atgtggcagc agcgcatgac
     5461 ggtaaagata ttgaatatga tacagagttt acaattgaca ataaagtcaa aaaaggcgat
     5521 acaatgacga ttaattatga taagaatgta attccttcgg atttaacaga taaaaatgat
     5581 cctatcgata ttactgatcc atcaggagag gtcattgcca aaggaacatt tgataaagcg
     5641 actaagcaaa tcacatatac atttacagat tatgtagata aatatgaaga tataaaagca
     5701 cgtttaactt tatactcata tattgataag caagtagtac ctaatgaaac tagtttgaat
     5761 ttaacgtttg caacagcagg taaagaaact agccaaaacg tttctgttga ttatcaagac
     5821 ccaatggttc atggtgattc aaacattcaa tctatcttta caaagttaga tgaaaacaaa
     5881 caaactattg aacaacaaat ttatgttaat cctttgaaaa aaacagcaac taacactaaa
     5941 gttgatatag ctggtagtca agtagatgat tatggaaata ttaaactagg aaatggtagt
     6001 accattattg accaaaatac agaaataaaa gtttataaag ttaaccctaa tcaacaattg
     6061 cctcaaagta atagaatcta tgattttagt caatacgaag atgtaacaag tcaatttgat
     6121 aataaaaaat catttagtaa taatgtagca acattggatt ttggtgatat taattcagcc
     6181 tatattatca aagttgttag taaatataca cctacatcag atggcgaact agatattgct
     6241 caaggtacta gtatgagaac aactgataaa tatggttatt ataattatgc aggatattca
     6301 aacttcatcg taacttctaa tgactctggt ggtggtgacg gtactgttaa acctgaagaa
     6361 aagttataca aaattggtga ctatgtatgg gaagacgttg ataaagacgg tgttcaaggt
     6421 acagattcaa aagaaaagcc aatggcaaac gttttagtta cattaactta cccggacggt
     6481 actacaaaat cagtaagaac agatgctaat ggtcattacg aattcggtgg tttgaaagac
     6541 ggagaaactt atacagttaa attcgaaacg ccagctggat atcttccaac aaaagtaaat
     6601 ggaacaactg atggtgaaaa agactcaaat ggtagttctg taactgttaa aattaatggt
     6661 aaagatgata tgtctttaga cactggtttt tataaagaac ctaaatataa tcttggtgac
     6721 tatgtatggg aagatacaaa taaagatggt atccaagatg ctaatgaacc tggtatcaaa
     6781 gatgttaagg ttacattgaa agatagtact ggaaaagtta ttggaacaac tactactgat
     6841 gcctcgggta aatataaatt tacagattta gataatggta actatacagt agaatttgaa
     6901 acaccagcag gttacacgcc aacggttaaa aatactacag ctgaagataa agattctaat
     6961 ggtttaacaa caacaggtgt cattaaagat gcagataata tgacattaga cagtggtttc
     7021 tataaaacac caaaatacag tttaggtgat tacgtttggt acgacagtaa taaagatggc
     7081 aaacaagatc caactgaaaa aggtattaaa gatgtgacag ttacattgca aaatgaaaaa
     7141 ggcgaagtaa ttggaactac aaaaacagat gaaaatggta aatatcgctt tgataattta
     7201 gacagcggta aatacaaagt tatctttgaa aaacctgctg gtttaacgca aacaggcaca
     7261 aacacaactg aagatgataa agatgctgat ggtggagaag tagatgtaac aattacagat
     7321 catgatgatt ttacaataga caacggatac ttcgaagaag aaacatcaga cagcgattca
     7381 gattcagata gcgactcaga ttcagacagc gactcagatt cagacagcga ttcagattca
     7441 gatagcgatt cagactcaga tagcgactca gactcagaca gcgattcaga ttcagatagc
     7501 gactcagact cagacagcga ctcagactca gacagtgatt cggactcaga cagtgattca
     7561 gactcagaca gcgactcaga ttcagacagt gactcagatt cagata
//
LOCUS       DAHHRR010000112         7580 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.112, whole genome shotgun sequence.
ACCESSION   DAHHRR010000112 DAHHRR010000000
VERSION     DAHHRR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.112"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..554
                     /gene="capA"
                     /locus_tag="NBI42_002152"
     CDS             <1..554
                     /gene="capA"
                     /locus_tag="NBI42_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HCD1778546.1"
                     /translation="LSPKYQANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKS
                     PRILDEVSKDLDDKYSPSKLSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVT
                     SKQIPKIMSVDNVSILSKADGTAVKVAPKTVVNLIGAFFLGLVIALIYIFFKVIFDKR
                     IKDEEDVEKELGLPVLGSIQKFN"
     gene            570..1256
                     /gene="cap8B"
                     /locus_tag="NBI42_002153"
     CDS             570..1256
                     /gene="cap8B"
                     /locus_tag="NBI42_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HCD1778547.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIDNLDLLTAGPVPPNPSELIGSERFKELVDMFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKTLMEKAGSNILGVILNKAKVDKS
                     SSYYHYYGDE"
     gene            1259..2023
                     /gene="cap8C"
                     /locus_tag="NBI42_002154"
     CDS             1259..2023
                     /gene="cap8C"
                     /locus_tag="NBI42_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HCD1778548.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMLDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVITGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2043..3866
                     /gene="cap8D"
                     /locus_tag="NBI42_002155"
     CDS             2043..3866
                     /gene="cap8D"
                     /locus_tag="NBI42_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HCD1778549.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVVVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQAGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGKIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMPNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEIMEMYKPYAVYHAAAHKHVPLMEDNPEEAVRNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHRTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITYTGIRPGEKMFEELMNKDEVHPEQVFEKIYR
                     GKVQHMKCNEVEAIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            3856..4884
                     /gene="cap8E"
                     /locus_tag="NBI42_002156"
     CDS             3856..4884
                     /gene="cap8E"
                     /locus_tag="NBI42_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HCD1778550.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            4897..6006
                     /gene="cap8F"
                     /locus_tag="NBI42_002157"
     CDS             4897..6006
                     /gene="cap8F"
                     /locus_tag="NBI42_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HCD1778551.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNKKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLRQYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNNRDVELTLNYVDDIIAEIKRAIEGNPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLASTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6010..7134
                     /gene="cap8G"
                     /locus_tag="NBI42_002158"
     CDS             6010..7134
                     /gene="cap8G"
                     /locus_tag="NBI42_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HCD1778552.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7137..>7580
                     /gene="cap8H"
                     /locus_tag="NBI42_002159"
     CDS             7137..>7580
                     /gene="cap8H"
                     /locus_tag="NBI42_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8H"
                     /protein_id="HCD1778553.1"
                     /translation="MRIAILGATNIKHMSLLSHYLNHIDLNINEVDIIYTDKYDIEEH
                     IQGINNYYKYKVDIKEDWTFIKKAIAYYRFRPYAMKILKENHYDFVIVWGSYTGHLFK
                     SFLEKHYKNKFILNIRDYFFENNKLIKYRMKKIVDASRLTTLSSEG"
BASE COUNT         2792 a         1067 c         1480 g         2241 t
ORIGIN      
        1 tcttatcacc taaatatcaa gctaatactc aaattttagt gaatcaaact aagggtgaca
       61 atcctcagtt tatggcgcaa gaggttcaaa gtaatattca acttgtaaat acgtataaag
      121 aaattgttaa aagtcctaga attttagatg aggtgtcaaa ggacttagat gataagtatt
      181 caccatctaa attatcgagt atgttgacaa ttacaaacca agaaaataca caacttatca
      241 acatccaagt taaaagtggt cataaacaag attcggaaaa aattgcgaat agcttcgcta
      301 aagttacaag taaacaaatt ccgaagatta tgagtgtgga taacgtatca attttatcta
      361 aagcagacgg tacagcagtt aaagtcgcac caaaaactgt agtgaatcta atcggtgcat
      421 tcttcttagg attagttatc gcgcttatat atatcttctt caaagtaatt ttcgataagc
      481 gaattaaaga tgaagaagat gtagagaaag aattaggatt gcctgtattg ggttcaattc
      541 aaaaatttaa ttaaggatgg ttgctactta tgtcaaaaaa ggaaaatacg acaacaacac
      601 tatttgtgta tgaaaaacca aaatcaacaa ttagtgaaaa gtttcgaggt atacgttcaa
      661 acatcatgtt ttcaaaagca aatggtgaag taaagcgctt gttggttact tctgaaaagc
      721 ctggtgcagg taaaagtaca gttgtatcga atgtagcgat tacttatgca caagcaggct
      781 ataagacgtt agttattgac ggcgatatgc gtaaaccaac acaaaattat atttttaatg
      841 agcaaaataa taatggacta tcaagcttaa tcattggtcg aacgactatg tcagaagcaa
      901 ttacgtcgac agaaattgat aatttagatt tactaacagc tggtcctgta cctccaaatc
      961 catctgagtt aattgggtct gaaaggttca aagaattagt tgatatgttt aataaacgtt
     1021 acgacattat tattgtcgat acaccgccag ttaataccgt gactgatgca caactatatg
     1081 cgcgtgctat taaagatagt ctgttagtaa ttgatagtga aaaaaatgat aaaaatgaag
     1141 ttaaaaaagc taaaacactt atggaaaaag caggcagtaa cattctaggc gtcattttaa
     1201 ataaggcaaa agtagataaa tcttctagtt attatcacta ttatggagat gaataagtat
     1261 gattgatatt cataaccata tattgcctaa tatcgatgac ggtccgacaa atgaaacaga
     1321 gatgctggat cttttaaaac aagcgacaac acaaggtgtt acagaaatca ttgtaacatc
     1381 acatcactta catcctcgat ataccacacc tatagaaaaa gtgaaatcat gtttaaacca
     1441 tattgaaagc ttagaggaag tacaagcgct aaatctaaag ttttattatg gtcaggaaat
     1501 aagaattacc gatcaaatcc ttaatgatat tgatcgaaaa gttattaccg gtattaatga
     1561 ttcacgctat ttactaatag aatttccatc aaatgaagtt ccacactata ctgatcaatt
     1621 atttttcgaa ttacagagta aaggctttgt accgattatt gcacatccag agcggaataa
     1681 agcaataagt caaaaccttg acatactata cgatttaatt aacaaaggtg ctttaagtca
     1741 agtgacaacg gcgtcattag cgggtatttc cggtaaaaaa attagaaaat tagcaattca
     1801 aatgattgaa aacaatctga cacatttcat cggttcagat gcgcataaca cagaaatcag
     1861 accgttctta atgaaagact tatttaatga taagaaatta cgtgattatt atgaagatat
     1921 gaacggattt attagtaatg cgaagttagt tgttgatgat aaaaaaattc ctaaacgaat
     1981 gccacaacaa gattataaac agaaaagatg gtttgggtta taaacagcaa atgaggggtt
     2041 ttatggcaca tttatctgtg aaattgcggc ttttaatact agcattaatc gattcactga
     2101 tagtgacatt ttcagtattc gtaagttatt acattttaga accgtatttc aaaacatatt
     2161 ctgtcaaatt attaatattg gcagctatat cactattcat atcgcatcat atttcagcat
     2221 ttatttttaa tatgtatcat cgagcgtggg aatatgccag tgtgagtgaa ttgattttaa
     2281 ttgttaaagc tgtgacgaca tctatcgtta ttacgatggt ggtcgtgaca attgttacag
     2341 gcaatagacc gttttttaga ttgtatttaa ttacttggat gatgcacttg attttaatag
     2401 gtggctcaag gttattttgg cgtatttatc ggaaatacct tggaggtaag tcatttaata
     2461 agaagccaac tttagttgtt ggtgctggcc aagcaggttc aatgttgatt agacaaatgt
     2521 tgaaaagtga cgaaatgaaa cttgaaccgg tattagcagt cgatgatgac gaacataaac
     2581 gcaatatcac aattactgag ggtgtaaaag tccaaggtaa aattgcggat attccagaac
     2641 tagtgaggaa atataagatt aaaaaaatca tcattgcaat tccaactatt ggtcaagagc
     2701 gtttgaaaga aattaataat atttgccata tggatggcgt tgagttattg aaaatgccaa
     2761 atatagaaga cgtcatgtct ggtgagttag aagtgaacca actgaaaaaa gttgaagtag
     2821 aagatttact aggcagagat cctgttgaat tagatatgga tatgatatca aatgaattga
     2881 cgaataaaac tattttagtt acgggtgcag gtggttcaat aggatcagaa atttgtagac
     2941 aagtttgtaa tttctatcca gaacgtatta ttctacttgg ccatggtgaa aacagtattt
     3001 atttaatcaa tcgggaattg cgaaatcgct tcggaaaaaa tgttgatatc gttcctatta
     3061 tagcggatgt gcaaaataga gcgcgtatgt ttgaaattat ggaaatgtat aaaccatacg
     3121 cagtttatca tgcagcagca cacaagcacg taccgttaat ggaagacaac cctgaagaag
     3181 cagtacgtaa taatatttta ggtacgaaaa atactgctga agctgctaaa aatgcagagg
     3241 taaagaaatt cgttatgatt tctacggata aagccgttaa tccgcctaat gtcatgggag
     3301 cttcaaagcg aattgcagaa atgattattc aaagtttaaa tgatgaaacg catcgaacaa
     3361 attttgttgc agtgagattt ggtaatgtac ttggatcgag aggatctgtg attccacttt
     3421 tcaaaagtca aattgaagaa ggtgggccag ttactgtgac acatcctgaa atgacacgtt
     3481 actttatgac aattcctgaa gcttctagac tagttttgca ggcaggggca ttagcagaag
     3541 gtggagaagt atttgtgcta gatatgggag aaccagtgaa aattgtagat ttggcacgta
     3601 atttaattaa gctaagtggt aaaaaagaag acgacatacg gattacttat acagggatta
     3661 gacccggcga aaaaatgttt gaagagctta tgaataaaga tgaggttcat cctgaacaag
     3721 tatttgaaaa aatttatcgt ggcaaagtac aacatatgaa atgtaatgaa gttgaagcga
     3781 ttattcaaga catcgtcaat gactttagta aagaaaaaat tattaactat gccaatggca
     3841 aaaagggaga taattatgtt cgatgacaaa attttattaa ttactggggg cacaggatca
     3901 ttcggtaatg ctgttatgaa acggttttta gattctaata ttaaagaaat tcgtattttt
     3961 tcacgcgatg agaaaaaaca agatgacatt cgaaaaaaat ataataattc aaaattaaag
     4021 ttctacattg gtgatgtgcg tgatagtcaa agtgtagaaa cagcaatgcg agatgttgat
     4081 tacgtattcc atgcagcagc tttaaaacaa gtgccgtcat gtgaattctt tccagttgag
     4141 gcagtgaaga caaatattat tggtacagaa aatgtcttac aaagtgcgat tcatcaaaat
     4201 gttaaaaaag tcatatgttt atctacagat aaggcagcgt atcctattaa tgctatgggt
     4261 atttcgaaag caatgatgga aaaagtattc gtagccaaat caagaaatat tcgtagtgaa
     4321 caaacgctta tttgcggtac aagatacggc aatgtgatgg cttcaagagg atcagtaatt
     4381 cctttgttta tcgacaaaat aaaagctgga gaacctttaa cgattacaga tcctgatatg
     4441 acaagatttt taatgagctt agaagatgcg gtagaactag ttgttcatgc atttaagcat
     4501 gcagagacag gcgatattat ggttcaaaaa gcaccaagct caacggtagg ggatcttgcg
     4561 accgcattat tagaattgtt tgaagctgat aatgcaattg aaatcattgg tacgcgacat
     4621 ggagagaaaa aagcagaaac attgttgacg agagaagaat acgcacaatg tgaagatatg
     4681 ggtgattatt ttagagtgcc ggcagactcc agagatttaa attatagtaa ttatgttgaa
     4741 accggtaacg aaaagattac gcaatcttat gaatataact ccgataatac acatatttta
     4801 acggtggaag agataaaaga aaaactttta acactagaat atgttagaaa cgaattgaat
     4861 gattataaag cttcaatgag ataggagaga ttgacgttga atattgtaat tacaggagca
     4921 aaaggttttg taggaaaaaa cttgaaagca gatttaactt caacgacaga tcatcatatt
     4981 ttcgaggtac atcgacaaac taaagaggaa gaattagaat cagcgttgtt gaaagcagac
     5041 tttgtcgtgc atttagcggg tgttaatcga cctgaacatg acaaagaatt cagcttagga
     5101 aacgtgagtt atttagatca tgtacttgat atattaacta gaaataagaa aaagccggcg
     5161 atattattat cgtcttcaat acaagcaaca caagataatc cttatggtga gagtaagttg
     5221 caaggtgaac agctattaag acaatatgcc gaagagtatg gtaatacggt ttatatttat
     5281 cgctggccaa atttattcgg caagtggtgt aagccgaatt ataactcagt gattgcaaca
     5341 ttttgttaca aaattgcacg taacgaagag attcaagtta ataatcggga cgttgaacta
     5401 acgctaaact acgtggatga tatcatcgct gaaataaaac gtgctattga aggaaatcca
     5461 acgattgaaa atggtgtacc tacagtacca aatgtattta aagtgacatt gggagaaatt
     5521 gtagatttat tatacaagtt caaacagtca cgtctcgatc gaacattgcc gaaattagat
     5581 aacttgtttg aaaaagattt gtatagtacg tatttaagct atctagctag tacagacttt
     5641 agttatccct tacttatgaa tgtggatgat aggggttctt ttacagaatt tataaaaaca
     5701 ccggatcgtg gtcaagtttc tgtaaatatt tctaagccag gtattactaa aggtaatcac
     5761 tggcatcata ctaaaaacga aaaatttcta gtcgtatcag gtaaaggggt aattcgtttt
     5821 agacatgtta atgatgatga aatcattgaa tattacgttt ctggcgacaa attagaagtt
     5881 gtagacatac cagtaggata cacacataat attgaaaatt taggcgacac agatatggta
     5941 actattatgt gggtgaatga aatgtttgat ccaaatcagc cagatacgta tttcttggag
     6001 gtatagcgca tggaaaaact aaaattaatg acaatagttg gtacaaggcc tgaaatcatt
     6061 cgtttatcat caacgattaa agcatgcgat caatatttta atcagatatt agtacacact
     6121 ggtcaaaatt atgattatac attgaatcaa attttctttg atgatttgga attaagacaa
     6181 ccggaccact acttagaggc tgttggaagt aacctcggag aaacgatggg gaatattatt
     6241 gcgaagacgt atgacgtttt attacgcgaa caaccagatg cacttttaat tcttggtgat
     6301 acaaatagtt gtttagcagc agtatctgct aaacgattaa aaatccctgt attccatatg
     6361 gaagcgggta atagatgctt cgatcagaat gtacctgaag aaatcaatcg taaaattgtt
     6421 gaccatgtca gtgatgtgaa tctaccttat acggaacata gcagacggta tttgttagat
     6481 gaaggcttca ataaagcgaa tatctttgtg acaggatcac cgatgacaga agtgatagaa
     6541 gcgcatcgag ataaaattaa tcacagtgac gttttaaata aactaggatt agaaccgcaa
     6601 caatacattt tagtatctgc gcatagagaa gagaatatcg ataatgaaaa gaattttaaa
     6661 tcattaatga atgcgataaa tgatattgct aaaaagtata aaatgcctgt gatttattca
     6721 acgcatccaa gaagttggaa gaaaattgaa gaaagtaaat ttgaatttga tccattagtt
     6781 aaacagttaa agccatttgg tttctttgat tataatgcat tgcaaaaaga tgcatttgtt
     6841 gtgctatcag atagtggaac attgtcagaa gagtcgtcta ttttgaagtt ccctggtgtc
     6901 cttattcgaa cttccacaga aagaccggaa gtactagata aaggtacggt tattgtaggt
     6961 ggtattacct ataacaatct aatccaatcc gttgaactag caagagagat gcaaaacaat
     7021 aacgaaccga tgattgatgc tattgattat aaagatacta acgtttcgac aaaagtagtt
     7081 aaaattattc aaagttataa agatattatc aatcgaaata cttggaggaa atgacgatga
     7141 ggatagcgat tcttggcgct actaacatta agcatatgtc attattatca cattatttaa
     7201 accacattga tttgaatatc aatgaggtgg acattatata cactgacaaa tatgatatcg
     7261 aagaacatat ccaaggcatc aataattact ataaatataa agtagatatt aaagaagatt
     7321 ggacatttat caaaaaagct attgcttact atcgatttag gccatacgct atgaaaattc
     7381 ttaaagaaaa tcattatgat tttgtcatag tatggggaag ttatacagga cacttattta
     7441 aaagtttttt agaaaaacac tataaaaata aattcatttt aaatataagg gactactttt
     7501 ttgaaaataa taaacttatt aagtatagaa tgaaaaaaat cgttgatgct agcaggctga
     7561 caacattatc ttcagaaggt
//
LOCUS       DAHHRR010000113         7569 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.113, whole genome shotgun sequence.
ACCESSION   DAHHRR010000113 DAHHRR010000000
VERSION     DAHHRR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.113"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            121..969
                     /locus_tag="NBI42_002160"
     CDS             121..969
                     /locus_tag="NBI42_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCD1778554.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEKELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            982..1809
                     /locus_tag="NBI42_002161"
     CDS             982..1809
                     /locus_tag="NBI42_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCD1778555.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTLVYLGVEHGLL
                     HNITTQIAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            1836..3155
                     /locus_tag="NBI42_002162"
     CDS             1836..3155
                     /locus_tag="NBI42_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCD1778556.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSCIKDIDGVKILFVAATAPFTPFYRALDWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVKTSFEEEGKTLMSNPVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLSGVKLKEIIAKSNKQEYMYEHAQGLGFRGNVFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            3239..4156
                     /gene="lipA"
                     /locus_tag="NBI42_002163"
     CDS             3239..4156
                     /gene="lipA"
                     /locus_tag="NBI42_002163"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCD1778557.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            4286..4669
                     /locus_tag="NBI42_002164"
     CDS             4286..4669
                     /locus_tag="NBI42_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCD1778558.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(4748..5014)
                     /locus_tag="NBI42_002165"
     CDS             complement(4748..5014)
                     /locus_tag="NBI42_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HCD1778559.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHGRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNSEEGDEITEYLNEVIH"
     gene            5112..5546
                     /locus_tag="NBI42_002166"
     CDS             5112..5546
                     /locus_tag="NBI42_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HCD1778560.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVDIRKQFTYDYIALD
                     IKVIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            5546..6325
                     /locus_tag="NBI42_002167"
     CDS             5546..6325
                     /locus_tag="NBI42_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCD1778561.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGFLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            6358..7317
                     /locus_tag="NBI42_002168"
     CDS             6358..7317
                     /locus_tag="NBI42_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HCD1778562.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKNQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTKILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFAQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCIKNIEAVMTNQVPHTPVN"
BASE COUNT         2718 a         1057 c         1421 g         2373 t
ORIGIN      
        1 aaacgaaatc atcaagctaa agttcactga aatgattata attaaacgaa attatcaaac
       61 taaagttcgc tgaaatgatt atgataaaag ttatatggta tgatgacatt ggtgatatat
      121 atgataaaca tcggattaac aggttggggt gatcactatt cattatatga agatttagaa
      181 cgccaaaccg ataaacttaa aacatatgct ggacattttc cggttgtcga attagatgcg
      241 acatactatg cgatacaacc ggaaagaaat atattgaaat ggataaaaga aacgcctgat
      301 acatttgaat ttgtggtcaa aattcatcaa gcactcacat tgcatgctga ttacaaaaca
      361 tttgcagata caaggcaaga actatttgat caatttaaga atatgttaga gcccttacat
      421 acacagaaaa aattagcaat ggtattggtt caatttccgc catggtttga ctgcaatgca
      481 caaaatatca aatatatttt gtatgtaaga cagcaattac aagcatttcc aatgtgtgta
      541 gaatttaggc atcaatcatg gtttagtgat gcatttaaag aacaaacatt ggcattttta
      601 acagaacatc aaatcattca tgcagtagtt gatgaaccac aagtgaaaga tggcagtgta
      661 cctttagtca atcgaatcac aaatgaaatt gcgtttgtac gttatcatgg acgtaatcat
      721 tacggttgga ctaagaaaga tatgtcagat caagaatggc gagatgtacg ctatttatat
      781 gattataatg agaaagaatt aatagacttg gcacaaaagg cacaaatatt agcacaaaaa
      841 gctaagaaag tttacgtcat atttaacaat aattctggtg gtcatgcagc aaataatgcc
      901 aaaacatatc agcgattatt gaatatagaa tatgaagggt tagcaccaca acaattaaaa
      961 ttattttaag aggcgacgac tatgttatta acaattacat tattagttct aatcggtggt
     1021 ttgtcagcaa tcatcggatc aatcgttggt attggaggcg gcattataat cgttccgaca
     1081 ttggtctatt taggtgttga acatgggtta ttacataata ttacaacgca aatagcgatt
     1141 gggacatcgt ctgtcattct aattgtgaca ggactttctt catcgcttgg atatctaaaa
     1201 acaaaacaag ttgatattaa aaatggctct atctttttat ttggattatt accaggttca
     1261 ttgcttgggt cattcattag tagatattta acatttgagt catttaattt atattttgga
     1321 atctttttaa tctttgtagc tattttatta atggtaagaa ataagattaa accgtttaaa
     1381 atatttgata aaccaaagta tgaaaaaact tatgtagacg ctaaaggtaa aacatatcat
     1441 tatagtgtgc caccattgtt tgcttttatt acaacgtttt taattggtat attgacaggt
     1501 ttatttggta ttggaggtgg cgcactaatg acgccactaa tgcttattgt atttagattt
     1561 ccacctcatg tagctgttgg aacaagtatg atgatgatat tcttttcaag tgtcatgagt
     1621 tctatagggc acattgctca agggcacgta gcttggggtt atgcaatcat tttaattatt
     1681 tctagttatt ttggtgcgaa aatcggtgtc aaagtgaatc aatcaattaa gtcagatacg
     1741 gtagtaacat tattgagaac agtaatgttg ttaatgggta tatatttaat tattcgtgcg
     1801 ttgatttaat acaactttaa aaggaggacg tcaatttgag gcttacaatt tatcatacga
     1861 acgatattca tagtcatctg catgaatatg aaagaattaa agcatatatg gcagaacatc
     1921 ggccacgact taaccatcct tctttatatg ttgatctagg tgatcatgta gatttatccg
     1981 cacctataac tgaagcaact ttaggtaaaa agaatgtggc attactaaat gaagcaaaat
     2041 gtgatgttgc aacaatcggt aataatgaag ggatgaccat ttcacacgaa gctttaaatc
     2101 acctttacga cgaagcaaaa tttatagtga catgtagcaa tgttatagat gaatcaggtc
     2161 atttaccaaa taatatcgtt tcttcttgta ttaaggatat agacggtgtg aaaatactat
     2221 tcgttgcagc gacagcacct tttaccccat tttatcgtgc actagattgg attgttaccg
     2281 atccacttga atctataaaa gaagaaattg aacttcaacg aggtaaattt gatgtattaa
     2341 tcgtgctaag tcattgtggc attttcttcg atgaaacatt atgccaagaa ttgcctgaaa
     2401 ttgatgtcat ttttggtagt catacgcatc attattttga acatggtgaa attaataatg
     2461 gtgtactgat ggcggcagct ggaaagtatg gtaattatct tggagaggtt aatttaactt
     2521 ttgaggcaca taaagtagta cataaaactg caaagattat tcctttagaa acattacctg
     2581 aagttaagac ttcatttgaa gaagaaggaa aaacgctaat gtccaatcca gtaattcaac
     2641 atccagtagt gcttaaacgt agtatgaacc acataactga agctgcatac ttattagctc
     2701 aaagtgtttg tgagtataca catgcacaat gtgctatcat caatgccggc ttactcgtta
     2761 aagatattgt aaaagatgaa gtgacagaat atgacattca tcaaatgtta ccgcatccga
     2821 ttaatatggt aagggttaga ctttctggtg tgaaattaaa agagattata gctaaaagta
     2881 ataaacaaga atatatgtat gaacatgcac aaggtttggg tttcagaggg aatgtatttg
     2941 gaggatatat tctttataat ttagggtaca ttcattctac agggcgttac tatctgaatg
     3001 gtgaagaaat cgaagacgac aaagaatatg tattaggtac gatagatatg tatacgttcg
     3061 gtcgttattt cccaacattg aaagaattac caaaagagta tttaatgcca gagtttttaa
     3121 gagatatatt taaagaaaaa ttattggaat attaaaaagt aagattattg gattttcatt
     3181 tgtcatgaat ttcgatataa tgtttaaaga tacacttaac aggagggtat gtgttgttat
     3241 ggcgacaaaa aacgaggaaa tattacgtaa accggattgg ttgaaaataa aattaaatac
     3301 caacgaaaac tatacaggac taaagaagat gatgagggaa aaaaatctta atactgtatg
     3361 tgaagaagct aaatgtccta atatacatga atgttggggt gcacgtcgta cagcgacatt
     3421 tatgatttta ggtgccgtat gtacaagagc ttgtcgtttt tgtgcggtta agacaggttt
     3481 acctaatgaa cttgatttaa atgagcctga acgtgtagct gaatcagttg aattaatgaa
     3541 cttgaaacac gttgttatca ctgctgttgc gcgtgatgat ttaagagatg ctggttcaaa
     3601 tgtttatgct gagacagtac gtaaagttag agaaagaaat ccatttacaa ctattgaaat
     3661 tttaccatca gatatgggcg gggactatga tgcgttagaa acattaatgg cttcaagacc
     3721 tgacatttta aaccataata ttgaaactgt tcgtcgatta acaccgagag ttcgtgcgcg
     3781 tgcgacttac gacagaacat tagagttttt acgtcgttca aaagaattac aaccggatat
     3841 cccaactaaa tcaagtatta tggttggatt aggtgaaact atagaagaaa tttatgaaac
     3901 gatggatgat ttacgtgcga atgatgtaga tattttaacg attggtcaat atttacaacc
     3961 ttcacgtaaa catttaaaag ttcaaaaata ttacacgcct ttagaatttg gtaaattaag
     4021 aaaagtggca atggataaag ggtttaaaca ttgccaagct ggacctttag tacgtagttc
     4081 atatcatgcg gatgagcaag taaatgaagc tgctaaagaa aagcaacgcc aaggtgaggc
     4141 acagttaaat agttaatatt taaccattaa taaggcataa aggcttagtt tgtacaaaac
     4201 gaacgtgtca tagaagtaat cattattttt atgaaacact aaaagtataa actgagcctt
     4261 tcattgtcat agataggtga agaatttgat aaaagtagat caacattact ttgaattaat
     4321 agaaaattat cgcgaatgtt ttaatgaaga acaatttatt gctaggtatt cagatatttt
     4381 agataaatat gattacattg ttggtgacta tggttacgat caattacgat taaaaggttt
     4441 ttacaaagat tctaataaaa aagcagagat gagtaaacgt ttttcaaata ttcaagatta
     4501 catatttgaa tattgtaact ttggttgtcc ttactttgta ttaagacatt tgtctaaaca
     4561 agaggttaaa aagttaatcg aagaagttca tccgtctgat gtgatagatg acgacaataa
     4621 acttcaagat gtgaagatta agccaaccat tcaagatact gaacattaat aaaaccctta
     4681 gctagattga aaatgggaat catgcaattc aagcatggac ctgtaatcta gttaggggtt
     4741 tttatcttta atgaatgact tcatttaaat actcagtaat ttcatcgcct tcttcagaat
     4801 ttacacctaa aatatgagcg atatagcctt cttcttttaa atcatcagta ccgataatac
     4861 cgaatttatt tgtttgcata ttaagtacga gtgtcttacc ataatgtcta tttgtatgga
     4921 ctaacattaa atcatatcga ccatgctcgc caacaaaacc aacaaactga acttgactct
     4981 cttcgttgtc atcatataaa tacatatcaa tcattttgta gcgactcctt ttaaaagtag
     5041 taaagttagt ataacgacaa atgaagtata ctgcaaaatt atgataatat ataagcgaga
     5101 ggtgacaagg aatgtatttt gtagacaaag ataaactaac tcagaaatta gcttatttac
     5161 aagcattaac tgatgattat catgagagta agcacaatca ttatgcattt gaacgcattg
     5221 ctcaaatgtt gatagaatca tcggtagata tagggaatat gattatcgat gcatttattt
     5281 taagggatcc tggtaattat aaagatgtga ttgatatatt agaactagaa aatgttatta
     5341 ctaaagaaac acagcaggcg attaataaaa ctgtcgatat tcgtaaacaa tttacatatg
     5401 attacatagc cttagatatt aaggttatca tgccaatgtt tgatgacgca ttaccttatt
     5461 acaaacaatt tattacagaa gtaacgacat ttttacatca agaaaatgta ccagtaacag
     5521 cttttggtaa aggagaaaat caataatgaa acagtataaa gcgtatttaa tcgatttaga
     5581 tggcacaatg tatatgggaa cagatgagat tgatggagca aaacaattca tcgattattt
     5641 aaatgtaaaa ggcattcctc atttatacgt aactaataat tcaacgaaaa cacctgagca
     5701 agtaactgaa aaattacgtg aaatgcacat tgatgctaaa ccagaagagg ttgtaacgtc
     5761 agcgttagcc actgctgatt atatttcaga acaatcacca ggagcatcag tatatatgtt
     5821 aggtgggagt ggtttaaata ctgcgttaac cgaagcggga cttgtcatta aaaatgacga
     5881 gcatgttgat tatgtagtta ttggacttga cgaacaagtt acatatgaaa agcttgcgat
     5941 tgcaacgtta ggtgtaagaa atggagcaac atttatttct acaaatccag atgtgtcaat
     6001 tccaaaagag cgaggatttt taccagggaa tggtgctatt acaagtgttg taagtgtatc
     6061 gacaggtgta tcgccacaat ttattggtaa accagaaccg attattatgg ttaaagcatt
     6121 agaaatttta ggattagata aatccgaagt tgctatggta ggcgatttgt acgataccga
     6181 tattatgtct ggtattaacg taggtatgga tacgattcat gtacaaacag gtgtatctac
     6241 gttagaagat gtgcaaaata aaaatgtgcc accaacgtat tcttttaaag atttaaatga
     6301 agcaatagct gaattagaaa aatagatata gtcattttat aaagtaggtg aattgatttg
     6361 gtaaaaatag ttgtttcgag gaaaattcca gataaatttt atcaacaatt aagtaaactt
     6421 ggtgacgttg ttatgtggca aaaatcatta gtgcctatgc ctaaaaatca atttgtgaca
     6481 gcgcttcgtg acgcagatgc ttgttttatc acattaagtg aacagatcga tgcagaaatt
     6541 ttagcacaat caccaaattt aaaagtaatt gcgaatatgg ctgtaggata tgacaacatc
     6601 gatgttgaaa gtgcaacagc gaacaacgtg gttgtcacga atacaccaaa tgtacttact
     6661 gaaacaactg cagaattagg atttacatta atgcttgcta tagcacgccg tattgtagaa
     6721 gctgaaaaat atgtagaagc agatgcatgg caaagctggg gtccttattt attgtcaggt
     6781 aaagatgtct tcaattcaac tattggaata tatggtatgg gagatattgg taaagctttt
     6841 gcaagaaggt tgcaagggtt taatactaaa attctttatc ataatcgatc aagacataaa
     6901 gatgcagagg cggactttaa tgcaacatat gtttcttttg aaacgttgtt agcagaaagt
     6961 gattttatca tctgtacagc accacttaca aaagaaacac atcataaatt taatgctgaa
     7021 gcatttgcac aaatgaaaaa tgatgcaatt tttattaata tcggcagagg gcaaattgta
     7081 gatgaaacag cattaatcga tgcactagac aataaagaaa tattagcatg tggtttagat
     7141 gtattagcaa atgaaccaat tgatcataca catccattaa tgggacgtga taatgttctg
     7201 attacaccac acattggtag cgcatcagta acaacacggg acaatatgat tcaattatgt
     7261 attaaaaata tagaagcggt tatgacaaat caggtaccac atactccagt aaattgaaaa
     7321 taagtatggt ttttaatcat tggtaaaaca agcaaagcaa ttgttaaata tgttatgctt
     7381 gacttgcgaa tgtatatatt tgcgctgatg ataattccat aacattataa actcgtcata
     7441 ttaatgtgat gagtttattt gattttgacg tatataacaa aattgttata tagactgtaa
     7501 ttgtttattg tttaaataac ctttaaaatg aatgatgttc tcaacaaagt gtatcattta
     7561 caataattg
//
LOCUS       DAHHRR010000114         7373 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.114, whole genome shotgun sequence.
ACCESSION   DAHHRR010000114 DAHHRR010000000
VERSION     DAHHRR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.114"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            312..941
                     /locus_tag="NBI42_002169"
     CDS             312..941
                     /locus_tag="NBI42_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HCD1778563.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQTKPQAPQQNSQSTTNKQNNANDK"
     gene            1583..2596
                     /locus_tag="NBI42_002170"
     CDS             1583..2596
                     /locus_tag="NBI42_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCD1778564.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            2649..3659
                     /gene="gap"
                     /locus_tag="NBI42_002171"
     CDS             2649..3659
                     /gene="gap"
                     /locus_tag="NBI42_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD1778565.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            3798..4988
                     /locus_tag="NBI42_002172"
     CDS             3798..4988
                     /locus_tag="NBI42_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCD1778566.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPDLGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSGSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            5110..5871
                     /locus_tag="NBI42_002173"
     CDS             5110..5871
                     /locus_tag="NBI42_002173"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCD1778567.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEEQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIAGLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            5874..>7373
                     /locus_tag="NBI42_002174"
     CDS             5874..>7373
                     /locus_tag="NBI42_002174"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCD1778568.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSTLHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREKKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGE"
BASE COUNT         2671 a         1132 c         1466 g         2104 t
ORIGIN      
        1 tgctttaatt ttcaatacgt agctataatt tgttgtaaaa tcaaaaagtt taatatgtta
       61 attttcaaaa aaaggctcaa aatatgtttg atttagttat taaatgttaa gatatataag
      121 actactattt ctttgtaaaa atgaatccga tttacgagtg agtaatagtg aatacagttt
      181 aaagttgaag aatgcaaaaa gagtaaatgt ttattaatat ttgtagaaac taggtaagca
      241 aattagttgt gaaaatgtta atggttgcgc gataatttct atatttaaat tagtttgaag
      301 tgagggagag tatgtcgaat caaaattacg actacaataa aaatgaagat ggaagtaaga
      361 agaaaatgag tacaacagcg aaagtagtta gcattgcgac ggtattgcta ttactcggag
      421 gattagtatt tgcaattttt gcatatgtag atcattcgaa taaagctaaa gaacgtatgt
      481 tgaacgaaca aaagcaggaa caaaaagaaa agcgtcaaaa agaaaatgca gaaaaagaga
      541 gaaagaaaaa gcaacaagag gaaaaagagc agaatgagct agattcacaa gcaaaccaat
      601 atcagcaatt gccacagcag aatcaatatc aatatgtgcc acctcagcaa caagcaccta
      661 caaagcaacg tcctgctaaa gaagagaatg atgataaagc atcaaaggat gagtcgaaag
      721 ataaggatga caaagcatct caagataaat cagatgataa tcagaagaaa actgatgata
      781 ataaacaacc agctcagcct aaaccacagc cgcaacaacc aacaccaaag ccaaataata
      841 atcaacaaaa caatcaatca aatcaacaaa caaaaccaca agcaccacaa caaaatagcc
      901 aatcaacaac aaataaacaa aataatgcta atgataagta gtatttagtc aaacaaaaat
      961 gaaccagtat gacagacaac acaattaatt gggttgtctc gaatattggt tcttattttt
     1021 ataattgtta attaagggag agatgatact taaatagtta gttgtttgtt ttgcggatag
     1081 tgaaatttat tttgagtgag gtgggacaga aatgatattt tcgcaaaatt tatttcgtcg
     1141 tccaccccaa cttgcacatt attgtaagct gacttttcgt cagcttctgt gttagggccc
     1201 ctgaatttaa ttgaaaaaag cttgttacaa gcgaaatttt gttcagtcaa ttactactaa
     1261 tgtaaatttt cgggttctag agcattgatt tatgtcctag tctcaaataa taagcaaatg
     1321 attagcgagt cgttataagg gtatacattt gactacagcg aataaaataa aacgttttgt
     1381 tgtataacaa atcgaaaata tactgcaagc gctttatcaa attatttaga aaatttaagt
     1441 tttatgcttg caatttttga aatagaatag tactattgca agtgtaaaga ggttaatttt
     1501 tgtcccacgc gggacttaaa aaggcaacca ctggttgtga tatatcctta tttacattta
     1561 taaatataag gaggaggtag tagtgaaaga cttattgcaa gcacagcaaa agcttatacc
     1621 ggatctcata gataaaatgt ataaacgttt ttctattctt actactatct caaaaaatca
     1681 gcctgtcgga cgtcgaagtt taagcgaaca tatggatatg actgaacgtg tactgcgttc
     1741 tgaaacagat atgcttaaga aacaagattt gataaaagtt aagcctaccg gaatggaaat
     1801 tacagctgaa ggtgagcaac tgatttcgca attgaaaggt tactttgata tctatgcaga
     1861 tgacaatcgt ctgtcagaag gtattaagaa taaatttcaa attaaggaag ttcatgttgt
     1921 tcctggtgat gctgataata gtcaatctgt taaaacagaa ttaggtagac aagcaggtca
     1981 attacttgaa ggcatattac aagaagatgc gatagttgct gtaactggcg gatccacaat
     2041 ggcatgtgtt agtgaagcaa ttcatttatt accatataat gtattcttcg taccagccag
     2101 aggtggacta ggcgaaaatg ttgtctttca ggcaaacaca attgcagcta gtatggcaca
     2161 acaagctggc ggttattata cgacgatgta tgtacctgat aatgtcagcg aaacaacata
     2221 taacacattg ttgttagagc catcagtcat aaacacttta gacaaaatta aacaagcaaa
     2281 cgttatatta cacggcattg gtgatgcgct gaagatggcg catcgacgtc aatcacctga
     2341 aaaggtcatt gaacaacttc aacatcatca agctgtcgga gaggcatttg gttattattt
     2401 tgatacacaa ggtcaaattg tccataaggt taaaacaatt ggacttcaat tagaagacct
     2461 tgaatcaaaa gactttattt ttgcagttgc aggaggcaaa tcgaaaggtg aagcaattaa
     2521 agcatacttg acgattgcac ccaagaatac agtgttaatc actgatgaag ccgcagcaaa
     2581 gataatactt gaataagaga taaaaagttt aatacttttt aaatatcatt ttaaaggagg
     2641 ccattataat ggcagtaaaa gtagcaatta atggttttgg tagaattggt cgtttagcat
     2701 tcagaagaat tcaagaagta gaaggtcttg aagttgtagc agtaaacgac ttaacagatg
     2761 acgacatgtt agcgcattta ttaaaatatg acactatgca aggtcgtttc acaggtgaag
     2821 tagaggtagt tgatggtggt ttccgcgtaa atggtaaaga agttaaatca ttcagtgaac
     2881 cagatgcaag caaattacct tggaaagact taaatatcga tgtagtatta gaatgtactg
     2941 gtttctacac tgataaagat aaagcacaag ctcatattga agcaggcgct aaaaaagtat
     3001 taatctcagc accagctact ggtgacttaa aaacaatcgt attcaacact aaccaccaag
     3061 agttagacgg ttctgaaaca gttgtttcag gtgcttcatg tactacaaac tcattagcac
     3121 cagttgctaa agttttaaac gatgactttg gtttagttga aggtttaatg actacaattc
     3181 acgcttacac aggtgatcaa aatacacaag acgcacctca cagaaaaggt gacaaacgtc
     3241 gtgctcgtgc agcagcagaa aacatcatcc ctaactcaac aggtgctgct aaagctatcg
     3301 gtaaagttat tcctgaaatc gatggtaaat tagatggtgg tgcacaacgt gttcctgtag
     3361 ctacaggttc attaactgaa ttaacagtag tattagaaaa acaagacgta acagttgaac
     3421 aagttaacga agctatgaaa aatgcttcaa acgaatcatt cggttacact gaagacgaaa
     3481 tcgtttcttc agacgttgta ggtatgactt acggttcatt attcgacgct acacaaactc
     3541 gtgtaatgtc agttggcgac cgtcaattag ttaaagttgc agcttggtat gataacgaaa
     3601 tgtcatatac tgcacaatta gttcgtacat tagcatactt agctgaactt tctaaataat
     3661 tttagtatag tttttattca aatacgctag tgctcagaac tatttagcat taattaaagc
     3721 ttatgagtaa gcggggagca caaacgcttc tccgcttatt tttatataaa atttcctaat
     3781 tacaaggagg aaacaccatg gctaaaaaaa ttgtttctga tttagatctt aaaggtaaaa
     3841 cagtcctagt acgtgctgat tttaacgtac ctttaaaaga cggtgaaatt actaatgaca
     3901 accgtatcgt tcaagctttg cctacaattc aatacatcat cgaacaaggt ggtaaaatcg
     3961 tactattttc acatttaggt aaagtaaaag aagaaagtga taaagcaaaa ttaactttac
     4021 gtccagttgc tgaagactta tctaagaaat tagataaaga agttgttttc gtaccagaaa
     4081 cacgcggcga aaaacttgaa gcagctatta aagaccttaa agaaggcgac gtattattag
     4141 ttgaaaatac acgttatgaa gatttagacg gtaaaaaaga atctaaaaat gatccagact
     4201 taggtaaata ctgggcatct ttaggtgatg tgtttgtaaa tgatgctttt ggtactgcgc
     4261 atcgtgagca tgcatctaat gttggtattt ctacacattt agaaactgca gctggattct
     4321 taatggataa agaaattaag tttatcggtg gcgtagttaa cgatccacat aaaccagttg
     4381 ttgctatttt aggtggagca aaagtatctg acaaaattaa tgtcatcaaa aacttagtta
     4441 acatcgctga taaaattatc atcggcggag gtatggctta tactttctta aaagctcaag
     4501 gtaaagaaat tggtatttca ttattagaag aagataaaat cgacttcgca aaagatttat
     4561 tagaaaaaca tggtgataaa attgtattac cagtagacac taaagttgct aaagaatttt
     4621 ctaatgatgc caaaattact gtagtaccat ctggttcaat tccagcagac caagaaggta
     4681 tggatattgg accaaacact gtaaaattat ttgcagatga attagaaggt gcgcacactg
     4741 ttgtatggaa tggacctatg ggtgtattcg agttcagtaa ctttgcacaa ggtacaattg
     4801 gtgtatgtaa agcaattgca aaccttaaag atgcaattac gattatcggt ggcggtgatt
     4861 cagctgcagc agcaatctct ttaggttttg aaaatgactt cactcatatt tcaactggtg
     4921 gcggagcgtc attagagtac ctagaaggta aagaattgcc tggtatcaaa gcaatcaata
     4981 ataaataata aagtgatagt ttaaagtgat gtggcatgtt tgtttaacat tgttacggga
     5041 aaacagtcac aagatgacat cgtgtttcat cacttttcaa aaatatttac aaaacaagga
     5101 gtgtctttaa tgagaacacc aattatagct ggtaactgga aaatgaacaa aacagtacaa
     5161 gaagcaaaag acttcgtcaa tgcattgcca acattaccag attcaaaaga agtagaatca
     5221 gtaatttgtg caccagcaat tcaattagat gcattaacta ctgcagttaa agaaggaaaa
     5281 gcacaaggtt tagaaatcgg tgctcaaaat acgtatttcg aagataatgg tgcgttcaca
     5341 ggtgaaacgt ctccagttgc attagcagat ttaggcgtta aatacgttgt tatcggtcat
     5401 tctgaacgtc gtgaattatt ccacgaaaca gatgaagaaa ttaacaaaaa agcgcatgct
     5461 attttcaaac atggtatgac accaattatt tgtgttggtg aaacagacga agagcgtgaa
     5521 agcggtaaag ctaacgatgt tgtaggtgag caagttaaga aagctgttgc aggtttatct
     5581 gaagagcaac ttaaatcagt tgtaattgct tatgaaccaa tctgggcaat cggaactggt
     5641 aaatcatcaa catctgaaga tgcgaatgaa atgtgtgcat ttgtacgtca aactattgct
     5701 ggcttatcaa gtaaagaagt atcagaagca actcgtattc aatatggtgg tagtgttaaa
     5761 cctaacaaca ttaaagaata catggcacaa actgatattg atggggcatt agtaggtggc
     5821 gcatcactta aagttgaaga tttcgtacaa ttgttagaag gtgcaaaata atcatggcta
     5881 agaaaccaac tgcgttaatt attttagatg gttttgcgaa ccgcgaaagc gaacatggta
     5941 atgcggtaaa attagcaaac aagcctaatt ttgatcgtta ttacaacaaa tatccaacga
     6001 ctcaaatcga agcgagtggc ttagatgttg gactacctga aggacaaatg ggtaactcag
     6061 aagttggtca tatgaatatc ggtgcaggac gtatcgttta tcaaagttta actcgaatca
     6121 ataaatcaat tgaagacggt gatttctttg aaaatgatgt tttaaataat gcaattgcac
     6181 acgtgaattc acatgattca acgttacaca tttttggttt attgtctgac ggtggtgtac
     6241 acagtcatta caaacattta tttgctttgt tagaacttgc taaaaaacaa ggtgttgaaa
     6301 aagtttacgt acacgcattt ttagatggtc gtgacgtaga tcaaaaatcc gctttgaaat
     6361 acatcgaaga gactgaagct aaattcaatg aattaggtat tggtcaattt gcatctgtgt
     6421 ctggccgtta ttatgcaatg gaccgtgaca aacgttggga acgtgaaaaa aaagcttaca
     6481 atgctattcg taactttgat gccccaactt atgcaactgc caaagaaggt gtggaagcaa
     6541 gttataatga aggcttaact gacgaattcg tagtaccatt catcgttgag aatcaaaatg
     6601 acggtgttaa tgatggagat gcagtgatct tctataattt ccgacctgat agagcagcac
     6661 aattatcgga aatttttgcg aacagagcat tcgaaggctt taaagttgaa caagttaaag
     6721 atttattcta tgcaacattc actaagtata atgacaatat cgatgcggct atcgttttcg
     6781 aaaaagtcga tttaaataat acaattggtg aaattgcaca aaataacaat ttaactcaat
     6841 tacgtattgc agaaactgaa aaatatcctc acgttactta ctttatgagt ggtggacgta
     6901 atgaggaatt taaaggtgaa cgtcgtcgtt tgattgattc acctaaagtt gcaacgtatg
     6961 acttgaaacc agaaatgagt gcttatgaag ttaaagatgc attattagaa gagttaaata
     7021 aaggtgactt ggacttaatt attttaaact ttgctaaccc tgatatggtt ggacatagtg
     7081 gtatgcttga gccgacaatc aaagcaattg aagcggttga tgaatgttta ggtgaagtgg
     7141 ttgataagat tttagacatg gacggttatg caattattac tgctgaccat ggtaactctg
     7201 atcaagtatt gacggatgat gatcaaccaa tgactacgca tacaacgaac ccagtaccag
     7261 tgattgtaac aaaagaaggc gttacactta gagaaactgg tcgcttaggt gacttagcac
     7321 ctacattatt agatttatta aatgtagaac aaccggaaga catgacaggt gaa
//
LOCUS       DAHHRR010000115         7335 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.115, whole genome shotgun sequence.
ACCESSION   DAHHRR010000115 DAHHRR010000000
VERSION     DAHHRR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7335
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.115"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            50..154
                     /locus_tag="NBI42_002175"
     CDS             50..154
                     /locus_tag="NBI42_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778569.1"
                     /translation="MLPFFIFDILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            129..443
                     /locus_tag="NBI42_002176"
     CDS             129..443
                     /locus_tag="NBI42_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778570.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFTHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(771..1574)
                     /locus_tag="NBI42_002177"
     CDS             complement(771..1574)
                     /locus_tag="NBI42_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1778571.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQEEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYADEITLDHNENGIPHALKKLL"
     gene            complement(1632..2546)
                     /locus_tag="NBI42_002178"
     CDS             complement(1632..2546)
                     /locus_tag="NBI42_002178"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCD1778572.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVAAKYSVDLPNLCIEMRSGIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFEDVKIIAKQHHAYATVISGAGPTILIFSRKENSGELVR
                     ALNRNVVTCHSELVDINVSGVKERIVYQ"
     gene            complement(2548..3609)
                     /locus_tag="NBI42_002179"
     CDS             complement(2548..3609)
                     /locus_tag="NBI42_002179"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCD1778573.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSEILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            complement(3615..4895)
                     /locus_tag="NBI42_002180"
     CDS             complement(3615..4895)
                     /locus_tag="NBI42_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCD1778574.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QAYAAVIVGLESSPEALITKHGYEVDKVYPVEGV"
     gene            5086..6468
                     /locus_tag="NBI42_002181"
     CDS             5086..6468
                     /locus_tag="NBI42_002181"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCD1778575.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DVKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRIIDQTLEHYIE
                     QLKNKPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYVSPKEAGIYVTDVPQQAQIL
                     DSAYEEIKKLRNYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATLYENF
                     TDVSGIFKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTYILHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRKKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            complement(6522..7097)
                     /locus_tag="NBI42_002182"
     CDS             complement(6522..7097)
                     /locus_tag="NBI42_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778576.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSVFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
BASE COUNT         2390 a         1321 c         1029 g         2595 t
ORIGIN      
        1 ctagtataaa gtgtattaac tagtattctg tttatatttt attatgacca tgttaccatt
       61 ctttattttc gatattttgc atatcgtgta taatatagat aacaataact tttcaggggt
      121 gaaattcaat gattggacaa acaaatttat ttgatgatgt actaaagcaa gatgagagat
      181 ttgttattgt agttcaagca ttagaagaaa agaacggaca attattaaag agaactttaa
      241 gggaatatcc cggtttaaac cataaacaaa tgaatgattt atttacgcac ttaaaggaat
      301 tattttccga agaatcattt gctgaaaacc aatcagcgtt cagtattaca gtttatacaa
      361 atttagatta tactgctgac caaatatatg ctcatgtaaa acgctttaga gggaagcatg
      421 actggacaca aacagctaaa taaaatcaag atgcttgcag tgattttaac aattaatgtt
      481 agattgtcac tacatttttt aatacattcg taattattca gaatgattca aatttattta
      541 tctacctccg tgactaatta atacttgatt tatttttaaa atatacatct attctattcg
      601 ttagatatca ttactaatct aaaatatgta tttcaaatct tccatagtat aactgaagtc
      661 attttcacat aaataaagct ctcttcaaag ctttcatttt ttcaatgtct actttgaagg
      721 gagcatttca atcttattca aatatcatgt gttaaggctt attttaaaat ttataacaat
      781 tttttgagcg catgtggaat accattttca ttatgatcca acgtaatttc atctgcatat
      841 tgcttaagtt catctgatgc attccccata gcaatagcta gacctgcgac ttcaaacatt
      901 gatttatcat tcatactatc accaaaagca atgacttgat ccaccgaata tcccaatttg
      961 tgacataacg ctttaattgc attacctttt gaaacatctt tggccataaa ttctaagaag
     1021 aatggcttac ttgtcgtagc atctacatta tcattgaaca caccattcaa atcaattcta
     1081 gcttcagtaa tattcgctac ataatcaact cccatgacct tgggtacatc gccttgaata
     1141 tatgctttga tatcatcaac acgtttcatc ggtaaaccag ttaattccgc ttcaatattc
     1201 atatattcat gttcgctgtc gtaaataatt tgaccatcat gatatgtaag aacgaaaaat
     1261 cctctatctc gacaataatc tacaatttca tcgaaatcct gcttaccaat cgatttactt
     1321 acttctactt cttcattagt catgttaatc gttttactgc cgttataact aataatatag
     1381 ctatgatgtt caggtaattt taaatctcta gcagttggaa tcataccttc agtaggtcta
     1441 ccagatgcta aaacaacata ataaccttct tcttgaattg ctgttaaata atttgcagtt
     1501 tcttctgata tcacattatc tgaatttagc aatgtgtcat ccatatctaa aacgacaact
     1561 ttataatttg tcatatcgca ttaaatctcc tctctatttc acattcaata ctattttaca
     1621 atataaagtg tctattggta tacaattcgt tctttaacac cactaacatt aatatctact
     1681 aattcagaat gacatgtaac tacatttcta tttaaagcgc gaactaattc gccactattt
     1741 tctttgcgac taaatattaa aatagttggt ccagcaccac taatcactgt tgcatatgca
     1801 tgatgctgct tagcaattat tttcacatct tcaaattcag caattaaatg ctgacgatac
     1861 ggttcatgaa agccatcttg ttgcatgagt ttacctgcta attcataatt gtgctgtgct
     1921 aatgcacaaa tcattgtatt actaattgca ctacttttaa ccgcttcact atgtgttaat
     1981 ttttgtggta aagcacgtct tgatgcttct gttttaagtt cataagttgg tatcgttaca
     2041 atcacatcca cgtctggtat gtcgatatgt gcaactgacg tttccttcga gacatcatta
     2101 taatatccag cgattaaacc accataaatg gtcggcgcaa cattatcagg atgtccttcg
     2161 atttcagtcg ctaattgtaa tacctcatgt ttagacagtt ggatatcacc aaaataattt
     2221 gcgatatata tagctcctac taaagcagaa gctgacgaac ctaaccccct tgccaatgga
     2281 ataccacttc tcatttcgat acataaatta ggcaagtcaa cactatattt agcagcaact
     2341 tgttgtgcca catgataaat aaagtttgtt tcgtccgtag gcaattgctt cgatgcatca
     2401 tcatggaaaa tatattccca ttttgtccct gatgtttcct ttacagacag atgcaaaaat
     2461 ttatctaaag ccatacctat agaatcaaag ccaactccaa ggttggctgt tgatgcagga
     2521 attgttaact ccaaaacatt cgacatgtta taaagctcct ttaatataat cgataatgct
     2581 atctttatca tttggcaatg gctttatcgg attatctagt agtgaaatag cagtatcagg
     2641 atcttttaat ccattaccag ttaaaatagc aactactttt ttaccttgag gtaatttacc
     2701 ttgtctatgc aatttaatta aacctgcaat agaagcatta cttgctggtt cactaaagac
     2761 accttcttta gttgtcatta actgatatgc ttctagaatt tcatcatcag taacactatc
     2821 tattaatcca tttgattctt taagagcatt agtcgcctta tcccaactag caggattacc
     2881 aattcgaata gcagttgcaa tcgtttcagg atttttaatg actttatttt ggacaattgg
     2941 tgatgcgcct tcagcttgga agccaaacat tttcggcaat tgtgatcctt tagcttcatg
     3001 atattcttta aagcctttcc aatatgcagt aatattacct gcattaccta caggaatcgc
     3061 taaaatatca ggtgcttcac cgtctaattg ttgcacaatt tcaaatgagc ctgtcttttg
     3121 cccttcgatt ctaaatggat tgacagagtt tacaagctcg atttcgccac tttttgcaat
     3181 ttcttttaca atttctaaag cttcatcaaa gtttccttca atagaaacga tttctgcacc
     3241 atacattact gcttgcgaca atttacctaa tgcaatttta ccttctggta ttacgacgat
     3301 agcttttaaa cctgctctcg cggcatatgc tgctgcagac gctgatgtat ttccagtcga
     3361 agcgcatatt acaattttct taccttgctc ttttgctttt gtcacagcca ttaccatacc
     3421 gcgatcttta aatgaacctg tcggattggc accttcatac ttcacatata aatctatacc
     3481 tagtatttca gacatatttt cacaatgaat gagtggtgta tttccctcgt tcaaagttaa
     3541 ttttggtgta ttttcattta ctggtaaatg tgctttaaac tcttctacta atccttgcca
     3601 tcttctcatt ataattaaac tccttctact gggtatactt tgtcaacttc gtatccatgc
     3661 ttagtgatta atgcttccgg tgatgattcc aatccaacta taacagcggc atatgcttga
     3721 ttatcgtaat ttgcaactct taatgatcgg tggaatggta atattgcctt taactcattt
     3781 tcaaaatttt caattgaacc tttgacatga ttcactacta caaagaaatt ggatttttct
     3841 ttaatattaa tttctgcatc agaatccatc atttcccttg ttttatctgt tttcaattca
     3901 aaatgtggtg gcaatgtgtg taaatctgat tcaaagaata atgctacatt caataaatca
     3961 ctgacaacgg cactacctgt tgctaaactg cctgctcctt ttccataaaa catcgtgtca
     4021 ccaacggcat caccaatgac ataaatcgcg ttatattcat cctctacagc tgctaattga
     4081 tgctttttat caattagcgt tggttctact gatgcattaa ctttgccatt ttcatatatt
     4141 cccttaccaa tcaatttaat tttatacccc agttgatcgg ctacattaat atcagttaaa
     4201 gttacaccac taattcctct tcgtttaacg tcgtttaatt taatgacttg gttaaatgat
     4261 aaatatgatg taatgacaac tttacgcgct gcatctaccc cttctacatc atcagttgga
     4321 tccgcttcag caaaaccaag tcttttcgct tcatcaagtg cttcctcaaa tgtcgtttgc
     4381 tctttagtca ttttagataa aataaaatta gaggtaccat ttaaaattcc cataaatttt
     4441 gaaatattat tcgcattcaa accattattt atggcattta cgatcggaat accacctgct
     4501 acactcgctt caaactttaa agctacacca ttttcttctg ctaaatcttc taaaagttta
     4561 agatgtactg ctaataaatc tttatttgcg gtaataacat gttttttatt tttaagtgct
     4621 gttcttaacc aatctacagt tggttcaatt cctcccatga cttcaacgat aatatctaat
     4681 gaatcatcat ttaaaatttc attaacatct tcagttaaat gatattggct aatatttagc
     4741 ggtctctttt tagatttatc tcgaacaaga atatgcttta tgacaatatc tttatttaat
     4801 gtatcttgaa tttgctgtcg attctcttcg atgattttaa caacaccaga tccgacagta
     4861 cctaatccta ataatgctat atttaatttt ttcattttat gtacctccgt ttgttctttt
     4921 gagaaaaaca aatgtattat tgaaaaacac tagatgatag tatagtatag aattattctt
     4981 tatttttgta aagttcgtaa tattcaaaat ataaatacat aatatcattc ttacttacaa
     5041 aactgaatac aattttctga ttttttagaa aggttggcat gcactatgaa agtttccaaa
     5101 ttcggtggta gctccgtctc caatgctact caaattaaaa aagttttaaa tattgttaat
     5161 tctgatcctg agcgaaaaat tgtaatcgtt tctgcaccag gaaaaagaca tgacaatgat
     5221 gttaaaacga ctgatttatt aatcagatta tatgaaaagg tcattaatca tcttgactat
     5281 catgataaaa aaagagaaat tattcagaga tatgatgata ttgtaaaaga attgcagatg
     5341 gatgaaagta ttttacgaat aatagaccaa acattagaac attatatcga gcaattaaaa
     5401 aataaaccta aaagattatt agatgcatta ctatcttgtg gggaagattt taatgcacaa
     5461 ttgattgctt tatataataa tagtcagggc attcctacaa aatatgtatc tccaaaagaa
     5521 gcgggtattt acgtcactga tgttccacaa caagctcaaa ttttagatag cgcttatgaa
     5581 gaaataaaga aattacgaaa ttatgatgag aaactaatta ttccaggatt ttttggtgta
     5641 tcacatgaag gctatatagt aacttttcct cgcggtggat ctgatataac tggagcaatt
     5701 atttcaagtg gcgttagagc tactttatac gaaaatttca ccgatgtgtc tggtatcttt
     5761 aaagctaatc ccaatatcat caaagatcct gaacttattg aagaaattac ttatagagaa
     5821 atgcgtgaat tgtcatatgc aggatttagc gttttccatg atgaagcatt gcaaccacta
     5881 tataaggatc gcattcctgt cgttattaaa aatacgaatc gcccccaaga taaaggtact
     5941 tacattttac atgaccgaga aatcaacgct aaaaatgtaa tcagcggtat tagttgtgat
     6001 aaagatttta ctgttattaa tatcaaaaaa tatttaatga acagacaagt aggtttcact
     6061 agaaaaattc tcggtgtctt agaagataac aatatttcat ttgatcatat gccctctggt
     6121 attgatacta taagtattgt gatgcgttca aaacaaattc aaaataaaga aacaaaagtt
     6181 ttaaatgaga ttcgtaaaaa atgcgatgtt gatgagctaa gtattgagca tgatttagca
     6241 attttaatga ttgtcggcga aggtatgaat aaagtcatcg gtactgctaa taaaattaca
     6301 catgccttag ctgaatcaaa aattaactta aaaatgatta accaaggtgc ttcggaaatc
     6361 tcgatgatgt ttggaattca tgtagatgat gctgaaaaag ctgtattatc tacatatgag
     6421 ttttgctatc acggggttgc attaaaaaac ttgtgtgata aaaactaata taaaagcaat
     6481 tcgtaacaca tgtattcaac tgcgttatga attgcttttt cttaattatt tattgttctc
     6541 tggattgcat ccttaatacg ttgctcacct actacaaatt ttaaaataaa aaattgatag
     6601 tagcttaatg catctatgac acgtttataa ttggaataat gatgatactg atggtgttgc
     6661 attaaatcat acattagaac gatctccggt ttaatataat ccacatccca atttaaacta
     6721 tggaagtaaa tgttactctt aggtatacgt atattcggat ccaatctaaa aatccattca
     6781 tcgtttacaa catcataaac gaaaacactc attattgttg tatcatcata ttgcacataa
     6841 acttttgcaa tttttgaaag tgataaatct tctttagcta ttgcttgatc ttgcttatct
     6901 atgtatagta acgcatatga ttgcggaata actttcacaa tatcacttaa acgcttacgt
     6961 tcaacgcata tttcaacaat agaaggtaat tttaattcat cattcaagaa aagttgcatg
     7021 gcaacttcac cgtgaaattt aaataattgc gatgatgttt tcattaaatt gtctactaat
     7081 tgttctttag catttatttc attcactctc aaatacacct cctaagcaag cgctttcaca
     7141 aatttcatta taccttttaa aattaacgaa ttcaatctcg aaagcatatc aattaataca
     7201 attcatttat aaaagttcag acatatcata aattcgtcat aaagttgaat tcacgaatct
     7261 ttcacgtcat attcattgaa catcaatttc tgtcagttta taatttgatt ataacaatgg
     7321 tgaattagaa tcaac
//
LOCUS       DAHHRR010000116         7332 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.116, whole genome shotgun sequence.
ACCESSION   DAHHRR010000116 DAHHRR010000000
VERSION     DAHHRR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.116"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(107..787)
                     /locus_tag="NBI42_002183"
     CDS             complement(107..787)
                     /locus_tag="NBI42_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCD1778577.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(859..1446)
                     /locus_tag="NBI42_002184"
     CDS             complement(859..1446)
                     /locus_tag="NBI42_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCD1778578.1"
                     /translation="MTINTFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     QFKIFVPDSPTAILFLVISISLILIRKQNSFIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMTWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(1436..2011)
                     /locus_tag="NBI42_002185"
     CDS             complement(1436..2011)
                     /locus_tag="NBI42_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCD1778579.1"
                     /translation="MSETLNQIKASFIEYLLFQYRFKSRIAVWVLNYLKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDD"
     gene            complement(2025..3269)
                     /locus_tag="NBI42_002186"
     CDS             complement(2025..3269)
                     /locus_tag="NBI42_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778580.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDFYFYLIETGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(3276..4574)
                     /gene="aroA"
                     /locus_tag="NBI42_002187"
     CDS             complement(3276..4574)
                     /gene="aroA"
                     /locus_tag="NBI42_002187"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCD1778581.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKSMNANIEGIEDNYTPLIIKPSVIKGIN
                     YKMEVASAQVKSAILFASLFSKEATIIKELDVSRNHTETMFRHFNIPIEAEGLSITTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(4584..5648)
                     /locus_tag="NBI42_002188"
     CDS             complement(4584..5648)
                     /locus_tag="NBI42_002188"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCD1778582.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIKYIGTYLNQFDQSFLLIDEYVNQ
                     YFANKFDDILSYENVHKIIIPAGEKTKTFHQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFVAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQQFKDREILQSLNGMDKYIAKGI
                     ETKLDIVVADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLRQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            complement(5674..6840)
                     /gene="aroC"
                     /locus_tag="NBI42_002189"
     CDS             complement(5674..6840)
                     /gene="aroC"
                     /locus_tag="NBI42_002189"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCD1778583.1"
                     /translation="MRYLTSGESHGPQLTVIVEGIPANLEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDRDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
BASE COUNT         2387 a         1371 c         1008 g         2566 t
ORIGIN      
        1 aataatctag cattagaaaa ataagcacaa aaaaactagc aatcacacaa aatgtgatta
       61 gctagttaat aagtgtctaa tttaagttaa ttgttaatct ataagattaa tcacttgaac
      121 gcgcaatcaa aataatacgt acaagctctg ctacagcgac agcagttgct gcaacataag
      181 tcattgctgc tgcagataat actttacgcg catgtttgta ttccttttca tttacaatgt
      241 tcaatgccgt aatttgtttc atcgctcttg aactcgcatc aaactcaact ggtaacgtaa
      301 caattgagaa taataccgct aatgacatta aaccagcacc aatccataaa gcagttgaac
      361 caaatgcact acctatcgct gttaagataa tacctaacat gatgatcata taacttaatg
      421 aactccctag gtttgcaaca ggtactaatg ctgctctgaa tcttaagaac caatatcctt
      481 ggtgatcttg aatggcatga ccaacttcgt gggctgcaat tgcagttcca gcaactgatg
      541 gtctgtcata gtttgcagga gatagtgaaa caactttctt tttaggatcg taatgatctg
      601 ttaagaatcc ttcaccttta acaacttcga catcataaat accgtttgca tgtaaaattt
      661 ctaatgcaac ttcacgaccc gttttaccac tagttgatct aacttgtgaa tatttctcat
      721 agttagattt aactttgtgt tgtgcccata aaggaagcac cattaatatt acgaaataaa
      781 ttatcatagt aaaaattgaa gacaataaac tcactctcct ttataaatat tttactgtca
      841 tttgccgttt ttatcaaatc atttacactt taataatttg tttaattcaa tataaagcaa
      901 aagtccaaaa acactcagac aacatgataa tacaccaatt tgccacacat gtgtagttat
      961 aaaatcataa tatggaaatt gaaggtgaaa atagtcaata taatcattca aaaataccca
     1021 agtcatcgct acactgattc caatcataga acgtttaaac ctaggataga agtaaattgc
     1081 ctgaacagcc attatactgt gggaaaacat taataccagg ccatttactg taatatcacc
     1141 ttgttcaata ataaataata tattcataat aactgcccaa atcccatatt tgaataatgt
     1201 tacaaatgcc agtgcatcga taaaactatt ttgttttcgg attaatatca atgagataga
     1261 aataactaag aataatattg cagttgggct atctggaaca aaaatcttaa actgccacgg
     1321 cgtatgactt aattgttcac cataccatat ataaccataa atcatcccta atatattaca
     1381 aatgagtagc atcattaacc aagaacgttg ataaagtgta tactgccaaa atgtattaat
     1441 cgtcatctgc taagtcctca aattgattat gtttatttac tagcttgagt gtatttaaaa
     1501 tttgcgttag ttgataaaaa cgttgctttt cattcatctg taaacttaaa tcaatattat
     1561 gtagcaagta atctattaat aacgcatgtt tatgccgatc tatagccata ctatttaaat
     1621 catgaagata agtttgatag ctgggcgatt ccgttagttg agcaactatt aaatcatcta
     1681 gcctttgctc acgctttgac acgtttgcga agtgaatttg aatatcaaaa gcacagttat
     1741 gattagcgat ataatcaaat atttcatttg tattcattaa ctttatatta cgcttagtaa
     1801 attgaattgc agaagcgtga cttcccactt ctgcaatttc taatgtttca tgatgattaa
     1861 tttttgtatc tacaaaatga atgtttgcca atttcgcctc attcactttt agatagttaa
     1921 gcacccaaac tgcaatacgc gacttaaatc gatattgaaa aagtaaatat tcaataaaac
     1981 ttgctttaat ttgattgagc gtctctgaca tcaaataccc cattttaaga ttgcaatctt
     2041 gataattcgt catgccaatt ttcattactt ggttctagtt ccaacaattg atttaaaata
     2101 gtaattgctt gttccttttg accagtttca attaaataga aataaaaatc actcataaaa
     2161 tcaatatttg ttttcattgt tggatatgct aattcaaaga aatgttgagc ttctttatct
     2221 cgctcttctt gaccataggc gaacgctaaa tgccacatga atgtaggatc caaatcttct
     2281 tcatctacat atgttaataa ttcaatgatt gcttcataat cttcttcatt acgatataaa
     2341 tcacttaaaa tcaataaagg ttcttggtaa gcattatcaa cctctaatgc ttgttttaac
     2401 aataatacac cttcattagc atcgccgtgt tctatttcca aacatccagt tgtatacatt
     2461 aactctttat aaaattgact tagtcgtaat ccttctttac ctgtttcaat ggcatctgga
     2521 taattttttt cattttcata taatgattgt aaatacaagt agccttgaat ataatcagga
     2581 tctttagaaa gtaatgtagt cattatttta attgcttctt gagtgatgtc atttttatcg
     2641 taagaaatgg cttttttgag ataatcttct gaagtcattt catcttcatt aatttcatcg
     2701 tataagcgaa tcgcatcact atagttacca ctttgtaaac tacaatctgc catacgagag
     2761 aataagttta caccattaac ttgatattca ccagtttcta aaacagtttc gtattcagag
     2821 gtagcacgta aatattgacc atcataatat aacatttcag ccaacgcaaa atggattatt
     2881 ggatcatttg gctctagttc aagtgcttct tgtaatttat caatagcaac ttccatcata
     2941 ttaatttgtt gatataaatc tgcttctaac atcaactttt caggtgatgg ttcaacataa
     3001 cttaaatatt ctaacgcttc gtcagtttga ttttcagaca ttaaaccttc aataaaataa
     3061 atcagcaatt cactttcgtc tggatatttg tgatataaca cgcggaatac ttccaaacct
     3121 tgtggcatta atccaaaatt gtaaagtgtc tctcctagaa taaataatgc gtcatcgttg
     3181 tcagtagtta ttgcttcatt aacacgagag tctaaatttt ctagtttttg tagattgata
     3241 tcgtctatta atttatagat atcttccatt ttatattatc cctcattttc taaaagcttt
     3301 agttttggta aaaatcctgg aaatgataca tttacagcat caaattgttt aattttgaca
     3361 ggttcgcttg aaagtagaga agcaactgca agcatcattc ctattcgatg atcagttaaa
     3421 ctatccactg ttgcatttgt tttaaattct gacggatgaa taatcaatcc atcattagtt
     3481 ggttgtaatt caaaccctaa caagtttaac atatcagccg ttgtatcaat tctatttgtt
     3541 tcttttactt ttaattcctc ggcatcttta attgtactcg tgccaactgc ttgtgtacaa
     3601 agtaatgcta ttacaggcag ttcatcaatt gcttttggca ctaattcacc ttcaattgtt
     3661 attggttgaa gcattggtgt gtattgaata cgaatagaag cagtaggttc agcaccagtt
     3721 gtttgattga aaagttgaat attaccgccc attttttcaa caatatcaat aatacctgaa
     3781 cgtgttggat tgattccaac attatgaatt gttacatcac ttcctggtgt gataagtgct
     3841 gcaacaataa agaacgctgc agatgaaata tcgccaggaa catgaaaatc tgcaggttta
     3901 atgtatcgaa ttgcttcagg ggttgtagta attgataacc cttctgcttc aattggaata
     3961 ttaaaatgtc tgaacatcgt ctcagtatga tttcgactta catctaattc tttaatgatg
     4021 gtcgcttcct tagaaaatag acttgcgaat aagatggcac ttttcacttg tgcacttgcc
     4081 acttccatct tgtaattaat acctttaatt acagatggtt taatgattaa tggggtgtaa
     4141 ttgtcttcaa ttccttcaat atttgcattc atacttttta acggtcttaa aacacgatcc
     4201 atcggccttt tacctatgga aacatctcca gacaagacac tttcaatacc taaaccactt
     4261 aacaaacctg ccaataatcg tgttgtcgta ccagaattac ctgtatacaa aacttgatgt
     4321 ggcgtgttaa aagattgata tcctggggaa gtcacaacta atttttcatc atcttctttg
     4381 atttctacac ctaacagtcg gaaaatatcc atcgtacgac gacaatcttc gccaagtagt
     4441 ggcttatata tagtagatac accttcagct agcgacgcca acatgattgc acggtgtgtc
     4501 attgatttat cgcccggcac ttctatttcg ccctttaacg gacctgaaat atcaatgatt
     4561 tgttcattta ccatttcatt cacctactta aaatatgttt ttaattgttc acatgcatgt
     4621 tgtaatgtta gttgatcaac atgttgtaca acgatatctc caaattgtct aatcaagacc
     4681 atttgtacac cttgcttatc attcttttta tcacttagca tatattggta taacgtttca
     4741 aaatccaagt cagttatcat gtctaaagga tagccgagtt gtcttaaata ttgaatataa
     4801 tgattaatat catgcttaga atcaaacaaa gcattcgcaa ctataaattg atagataatg
     4861 ccaaccatca ctgcatgacc atgaggtatt ttatgatagt attcaacagc atgcccaaat
     4921 gtatgaccta aatttaaaaa tttacgtaca ccttgttctt tttcatctgc aacaacaata
     4981 tctagcttcg tttcaatacc tttagcaata tatttatcca taccatttaa tgactgtaat
     5041 atctctctat ctttaaactg ctgttcgata tcttgcgtcg ctgattcacc attcaataac
     5101 gcatgcttat aaacttctgc atagccactt aatatttgct caaatggtaa cgtctttaaa
     5161 aagtctaaat cataaatcac agcagttgga cgataaaatg caccgataag gtttttacct
     5221 tgctttgagt taatacccac tttaccgcca acactagaat catgcgccaa aatcgttgtg
     5281 ggaacttgta taaaatggac acctcttaat agtgttgctg ctacaaatcc tgcaaaatct
     5341 cctgtcgcac caccaccaac agcaataatt gctgtattac gagtcacatg atgggataaa
     5401 atatactcta atgtttcttg atattgatga aatgtttttg tcttttcacc tgctggaata
     5461 atgattttat gcacattttc atatgataaa atatcatcaa atttattcgc aaaatattga
     5521 tttacatatt catcaattaa caagaaactt tggtcaaatt gatttaaata tgtgcctata
     5581 tacttgattg caccgtgttc aacatatatt ggataattgt ttgaagggta tgttgtttgt
     5641 aatttcatga ttacacctca attgttcttg ttgttaaaac tcaatattta attgtctgcg
     5701 ctcaataatt tgttgtttaa gttgctcaat atgatttgat tggaattctt ccaataatgc
     5761 ttttgcaatt tcaaatgcta cgacatgttc acagacgata cttgctgcag gaacagcaca
     5821 actatcagaa cgttcaattg ttgctttaaa gtcttcttta gtattaatgt ctactgaatt
     5881 taatggttta tataacgttg gaattggttt cattacacca tttacaataa taggcatacc
     5941 atttgacata ccaccctcta aaccacctaa atgattcgat ccacgataat aaccaatttc
     6001 actattataa agaatttcat cttgaatctc actacctggc ttttcagctg ccttaaatcc
     6061 ttcaccaaag cttacacctt taaaagcatt tatgctgaca acgccttgtg caatcttacc
     6121 atctaactta cgatcataat gcacataact accaacaccg acaggcatat tttcaaccac
     6181 aacttgaacg acaccgccaa ttgaatctcc ttcattttta gcttcgtcaa ttttatctcg
     6241 cattgcttgc gcgatactgt catcaattac acgaacatcg ttacgatcaa gatttgcttt
     6301 aaatgtttct gaatcataaa aatctctatc tttaattcca cctatttcaa caacacgact
     6361 gtatatatcg atatctaact gttgtaataa cactttacat aaggcaccga ctgcaactcg
     6421 agctgctgtt tctctagcag atgatcgctc tagcacattt cgtaaatcac gatgattata
     6481 tttcatacct ccaaccaaat ctgcatgacc aggtcttggt tttgtaatag tacgtttcat
     6541 attttcacgt tcttcttcac ttattggagc cgctcccata atttttctcc aatgtgtaaa
     6601 gtcatcattg gttacaacca tagtaattgg actacctaat gtataaccat ttctaacgcc
     6661 tgatactatt tctactgtat ctttctcaat ttgcatgcgt cggccacgac catatccacc
     6721 ttgacgctta aacatttctt tattaatatc ttcaacttta atttctaaat ttgctggtat
     6781 accttcgaca ataactgtta attgaggtcc atgtgattct cctgaagtta gatatctcat
     6841 acacactcgc tcctttcata cttccgcaat aacattataa tatgttgtta atcttatcat
     6901 attcattgtg cattcataaa tgtttgtttt caagctcaat taagaaattg aattatatct
     6961 ctttttaaat gtcagaatta tcttaatatt aaacgaacta agattcatta taatatattc
     7021 gtctgtagaa aagtatcatg aatttctaat acagttgcat catataatca ttaattaaaa
     7081 ataattagcc gttttaatat aatcgtatcg caacttttgc aaataatata ctgactacat
     7141 ttcttaatac aaaagaacga actgttaatt attaataaag ttaatcagtc cgtataaaaa
     7201 tagtttatga tattaaaaat acgcatatgt taagcgcttt tgttatactt cgacttcaaa
     7261 aatataatca actcttttac taatacataa aaaatataaa tgagagtcag tataaaaata
     7321 atttttgtta aa
//
LOCUS       DAHHRR010000117         7155 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.117, whole genome shotgun sequence.
ACCESSION   DAHHRR010000117 DAHHRR010000000
VERSION     DAHHRR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.117"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            445..1104
                     /gene="arlR"
                     /locus_tag="NBI42_002190"
     CDS             445..1104
                     /gene="arlR"
                     /locus_tag="NBI42_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HCD1778584.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            1101..2456
                     /gene="arlS"
                     /locus_tag="NBI42_002191"
     CDS             1101..2456
                     /gene="arlS"
                     /locus_tag="NBI42_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCD1778585.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIDYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2740..5538
                     /locus_tag="NBI42_002192"
     CDS             2740..5538
                     /locus_tag="NBI42_002192"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCD1778586.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPSLKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     TLLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5552..6850
                     /gene="sucB"
                     /locus_tag="NBI42_002193"
     CDS             5552..6850
                     /gene="sucB"
                     /locus_tag="NBI42_002193"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCD1778587.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAVIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNKSADNAEVNQTNDDNQQRVNATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQAPASTQTTQQAPAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLL
                     EVSNNTAMLTTFNEVDMTNVMELRKRKKDQFMKDHDGTKLGFMSFFTKASVAALKKYP
                     EVNAEIDGDDMITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKARE
                     KKLGLDDMVNGSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENR
                     PMMYIALSYDHRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         2692 a         1061 c         1289 g         2113 t
ORIGIN      
        1 ttcctatttt taagtacaca ttagctgtgg ctaatgtgta agaaccacta cataataaat
       61 cattagtggc tctttatcat ttctgtccca ctcccaaatg tttaaactac ggtcattttg
      121 tgaaaataaa gataataact attcatttta cattagtaaa agtcagtaaa cggcaacaat
      181 tggtattgcg gtaaggcctt gtgttacagt attctatatt taagtactgc aatcagaatt
      241 aacagaattc cattaactga ttattaaata tttgagttaa taaataatta atgattgtag
      301 cttgaaaaat ttaaaacatg gttattgatt tgtgataaaa tttaaacgta aacaaactaa
      361 tttaaaaagc aactattgta tagaaaaata caaaatttaa aatatattac cttattagaa
      421 aaagtcgtaa tatgaggtgt acaaatgacg caaattttaa tagtagaaga tgaacaaaac
      481 ttagcaagat ttcttgaatt ggaactcaca catgaaaatt acaatgtgga cacagagtat
      541 gatggacaag acggtttaga taaagcgctt agccattact atgatttaat catattagat
      601 ttaatgttgc cgtcaattaa tggcttagaa atttgtcgca aaattagaca acaacaatct
      661 acacctatca ttataattac agcgaaaagt gatacgtatg acaaagttgc tgggcttgat
      721 tacggtgcag acgattatat agttaagcca tttgatattg aagaactttt agcaagaatt
      781 cgtgcgattt tacgtcgtca gccacaaaaa gatattatcg atgtcaacgg tattacaatt
      841 gataagaatg cttttaaagt gacggtaaat ggcgcagaaa ttgaattaac aaaaacagag
      901 tatgatttac tatatcttct agctgaaaat aaaaaccatg ttatgcaacg ggaacaaatt
      961 ttaaatcatg tatggggtta taatagtgaa gtagaaacaa atgtcgtaga tgtttatata
     1021 agatatttac gaaacaaatt aaaaccatac gatcgtgaca aaatgattga aacagttcgt
     1081 ggcgttgggt atgtgatacg atgacaaaac gtaaattgcg caataactgg attattgtta
     1141 ccacgatgat tacgtttgtc acgatatttt tgttttgttt aattattatc tttttcttga
     1201 aagatacact gcataatagt gagcttgatg atgcagaacg aagctcaagc gatattaata
     1261 atttatttca ttctaagcct gttaaagata tatctgcatt agacttgaat gcatctttag
     1321 gtaattttca agagattatt atttatgatg agcataataa taaattattt gagacatcga
     1381 atgataacac agtgagagtt gaaccaggtt atgaacaccg gtattttgac cgcgtaataa
     1441 aaaaacgtta taaaggcatt gattatttaa ttattaaaga accaatcaca acgcaagatt
     1501 tcaaagggta tagcttgtta attcattcac tagaaaatta tgataacatc gtaaaatcat
     1561 tgtatatcat tgcgctcgca tttggagtga ttgcaacaat tataactgcc acaatcagtt
     1621 atgtattttc aactcaaata actaaaccgc ttgtcagttt atcaaataaa atgattgaga
     1681 ttcgacgaga tggttttcaa aataaattgc aattaaatac aaattatgaa gaaatagata
     1741 atttagcaaa tacgtttaat gagatgatga gccaaattga agaatcattt aatcaacaaa
     1801 gacaatttgt tgaagatgcg tcacatgaat tacgaacacc attacaaatt attcaaggtc
     1861 atttaaattt gattcagcga tggggaaaaa aagacccagc agtattagaa gaatcgttaa
     1921 atatttctat tgaagaaatg aatcgtatca taaaattagt cgaagaatta cttgaattga
     1981 ctaaaggaga tgtaaatgac atttcttctg aagcgcagac cgtgcatatt aatgatgaaa
     2041 ttcgctcgcg aatacactca ttaaaacaat tgcatcctga ttatcaattt gacacggatc
     2101 tgacatctaa aaatctagaa attaaaatga aacctcatca attcgaacaa ttatttttaa
     2161 tctttattga taatgcaatc aaatatgatg tgaagaataa gaaaattaaa gttaagacaa
     2221 ggttaaaaaa taagcaaaaa ataattgaaa ttacagatca tggaattggt attccagagg
     2281 aagatcaaga tttcattttt gatcgttttt atcgagtgga taaatctcgt tcaagaagtc
     2341 aaggcggtaa tggactcgga ttatctattg cgcaaaaaat cattcaatta aacggtggat
     2401 cgattaaaat taaaagtgaa attaataaag gaactacgtt taaaatcata ttttaatcat
     2461 gtctgagacg tcaatcaaag tcataggatc aattttttaa gtacacatta gctgtgacta
     2521 atgtataaga acgactataa aacaaataaa cagtggttct ttatcatttc tgttgtactc
     2581 ccaaaattta caataaaata catctataaa cctagaagaa tcaacgcttt tgttgattct
     2641 tctttttagc atataaatag gtaaatctac tttaacaaat aactaaatag tgatattatt
     2701 acattgtaag cgtttcaaca tttttgtgga gggtgtaaaa tgactaacga aagaaaagaa
     2761 gtttcagagg ctcctgtaaa cttcggtgcg aatttaggtc taatgttaga tctatatgat
     2821 gactttttac aagatccatc atctgtacca gaagatttac aagtcttatt cagcacaatt
     2881 aagaatgatg actcaattgt accatcttta aaaagtacaa gtagtcaaaa tagcgacggc
     2941 acaattaaac gtgtcatgcg tttaattgat aatattcgcc aatacgggca tcttaaagcc
     3001 gatatttatc ctgtaaatcc tccgaaaagg aaacatgtac ctaaattaga gattgaagac
     3061 tttgatttag atcaacagac tttggaaggt atatcagcag gaattgtttc agatcacttt
     3121 gccgacattt atgataatgc ttatgaagca attttaagaa tggaaaaacg ttacaaagga
     3181 ccaattgcat ttgagtatac acatattaat aacaataccg aacgtggttg gttaaaaaga
     3241 agaattgaaa cgccatataa agtaacgtta aataataacg aaaaaagggc actattcaaa
     3301 caattagcgt atgttgaagg gtttgaaaaa tatcttcata aaaacttcgt tggtgcaaag
     3361 cgtttttcaa ttgaaggggt agacgcactt gtaccgatgt tacaacgtac tattacgatt
     3421 gctgcgaaag aaggtattaa aaatatacaa ataggcatgg ctcaccgtgg acgtttaaac
     3481 gttttaacgc atgtcttaga aaaaccgtac gaaatgatga tttcagaatt tatgcataca
     3541 gatccaatga aattcttacc tgaagatggt agcttgcagt taactgctgg atggactggt
     3601 gatgtgaaat atcaccttgg tggtattaaa actactgatt catacggtac aatgcagcgt
     3661 attgcactgg ctaacaatcc aagtcacttg gaaattgttg cacctgttgt tgaagggcgc
     3721 acaagagcag cacaagatga tacacagcgt gctggggctc cgacgactga tcatcataaa
     3781 gcaatgccaa ttattataca tggcgatgct gcttatcctg gtcaaggaat taacttcgaa
     3841 acaatgaact taggaaactt gaaaggctat tctacgggtg gttcattgca tattattact
     3901 aacaatagaa ttggatttac cacagaacca attgatgcac gctcaacaac ttattctaca
     3961 gatgtggcca aaggttatga tgtgccaata ttccatgtca atgcagatga cgttgaagct
     4021 actattgaag caattgatat tgcaatggaa tttagaaaag agtttcataa agacgtcgtt
     4081 attgatttag taggttatcg tcgtttcggg cataacgaaa tggatgaacc atcaattact
     4141 aatccagttc cttatcagaa tattcgcaaa catgactctg ttgaatatgt gtttggtaaa
     4201 aagcttgtta atgaaggtgt catttcagaa gatgaaatgc attcatttat agaacaggtc
     4261 caaaaggaac taagacaagc tcatgataaa attaataaag ctgataaaat ggataatcca
     4321 gatatggaaa agcctgcaga tcttgcatta ccgttacaag cagacgaaca atcatttacg
     4381 tttgatcact tgaaagaaat aaatgatgca ttgttaacat atccggatgg ctttaacatt
     4441 ttgaaaaagt taaacaaagt tcttgagaag cgtcatgagc catttaataa agaagatggt
     4501 ttagttgatt gggcacaagc agaacaactt gcatttgcga caattttaca agatggtaca
     4561 ccgattcgct taactggtca agatagtgaa cgtggtacat tcagtcatag gcatgccgtg
     4621 ttacatgatg agcaaacagg tgaaacatat acacctttac atcatgttcc tgatcaaaaa
     4681 gcgacatttg atatacacaa ttctccgctt tcagaagcag cagtagttgg ttttgaatac
     4741 ggctataatg tggaaaacaa aaaaagtttc aatatttggg aagcacaata tggtgatttt
     4801 gcaaatatgt cacaaatgat ttttgacaac ttcttattca gttctcgctc aaaatgggga
     4861 gaacgttcag gattaacatt attcttacct catgcatatg agggtcaagg acctgaacat
     4921 tcatcagcaa gattagagag atttttacaa ttagctgctg aaaataattg cacagttgta
     4981 aacttatcta gttcaagtaa ttatttccac ttattgcgtg cacaagcggc tagtttagat
     5041 tctgaacaaa tgcgaccatt ggttgttatg tcaccaaaaa gcttattgag aaataaaaca
     5101 gttgcaaaac caattgatga atttacttct ggtggatttg agccaatttt gacagaatca
     5161 taccaagcgg ataaggttac aaaagttatt ttagcaactg gtaaaatgtt cattgattta
     5221 aaagaagcat tagctaaaaa tccagacgaa tcagtattac tcgttgcgat tgaaagattg
     5281 tatccattcc cagaggaaga gattgaaaca ttgctagcac aattaccaaa ccttgaagaa
     5341 gtgtcatggg tacaagaaga acctaaaaac caaggtgcct ggttatatgt ttatccatat
     5401 gttaaagtgc tagttgcaga taaatatgat ttaagttatc atggcagaat tcaaagggct
     5461 gctccagctg aaggcgatgg agaaattcat aaacttgttc aaaataaaat tatagaaaat
     5521 gcattaaaaa ataactaggg ggaaataagt catgccagag gttaaagttc cagaattagc
     5581 agaatctatt acagaaggta ccattgcaga atggttgaaa aacgtagggg atagcgtaga
     5641 aaaaggtgaa gctattcttg aattagaaac tgataaagtt aatgtcgaag ttgtatctga
     5701 agaagcaggt gtattatctg aacaacttgc cagtgaaggc gacactgtag aagttggaca
     5761 agcaattgct gtcatcggcg aaggtagtgg caatgcttct aaagaaaata gtaacgacaa
     5821 tactccacaa caaaatgaag aaacaaataa taaaaaagaa gaaacaacaa ataaatcggc
     5881 agataatgct gaagtcaatc aaacaaatga tgacaatcag cagcgtgtta atgctacacc
     5941 ttctgcgcgt cgatatgctc gtgaaaatgg tgtgaatctt gctgaagtaa gtccgaaaac
     6001 aaatgatgtt gttcgtaaag aagatattga taagaaacaa caggcaccgg catcaacaca
     6061 aacaacacaa caggcaccgg catcaacaca aacaacacaa caagcacctg caaaagaaga
     6121 gaaaaaatac aatcaatatc ctacaaaacc agtgattcgt gaaaaaatgt cacgcagaaa
     6181 gaaaacagct gctaaaaaat tattagaggt atctaataat acagctatgt taacaacatt
     6241 taacgaagtt gacatgacaa atgttatgga attgcgtaaa cgtaagaaag accaatttat
     6301 gaaagatcat gatggtacta aattaggatt tatgtcattc tttactaaag cttctgtagc
     6361 agctttgaaa aagtatccag aagttaatgc agaaatcgac ggcgacgaca tgattacgaa
     6421 acaatattat gatattggtg tagctgtttc tacagatgat ggattattag taccattcgt
     6481 aagagattgt gataaaaaga attttgcaga aatcgaagca gaaattgcta atttagcagt
     6541 taaagcacga gagaaaaaac ttggcttaga tgatatggtt aatggttcat ttacgattac
     6601 aaatggcggt atttttggat caatgatgag tacgccaatt atcaatggta atcaagctgc
     6661 aatcttaggc atgcattcaa ttattacaag accaattgcg attgatcaag atacaatcga
     6721 aaatcgtcca atgatgtata ttgcattaag ctatgatcat agaattattg acggtaaaga
     6781 agcagttgga ttcttaaaaa caattaaaga attaattgaa aacccagaag acttattatt
     6841 agaatcttaa tccacaacac aaaatagttt actattccta aaaacgggat caaatcaata
     6901 acaaacagca gtaagattat tttctagtcg aaataactta ctgctgtact ttattttcac
     6961 aatgcttcgt attaaaatgt gagtttaatt ttgactactt aaaaaatata tcatttctat
     7021 tgaaatagac tcacaataga aatataggta gaatagcgtg tttcaatatg ctaatgaaat
     7081 cattttagat aataacaagc gatgaaattt atgaattcta taagcgcctt tagttaattt
     7141 aaaatcatct gaaca
//
LOCUS       DAHHRR010000118         7052 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.118, whole genome shotgun sequence.
ACCESSION   DAHHRR010000118 DAHHRR010000000
VERSION     DAHHRR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.118"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            186..836
                     /locus_tag="NBI42_002194"
     CDS             186..836
                     /locus_tag="NBI42_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HCD1778588.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSAKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTTNQSSKTQLTQKDENKNEDKPEEKSETKTDEDLQSTE
                     EVPANENTENNQHEIDEITTTDQSDDEINTPNVAEDKSQDDLKDDLNEKQQPSDHHQS
                     TQPKTSPSTETNKQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            917..1912
                     /locus_tag="NBI42_002195"
     CDS             917..1912
                     /locus_tag="NBI42_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCD1778589.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQHQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVNYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNSSKSDKSSVEKSESNEETAPVEPMPQSKPTTSEASPSQNIHNEDSMYDASTE"
     gene            1988..2614
                     /locus_tag="NBI42_002196"
     CDS             1988..2614
                     /locus_tag="NBI42_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778590.1"
                     /translation="MKFKAIVAITLSLSLLTACGANQHKENSSKSNDTNKKTQQTDNT
                     TQSNTEKQMTPQEAEDIVRNDYKARGANENQTLNYKTNLERSNEHEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGTIINIFDDMSEKDKEEFEAFKKRSPKYNPGMSDQAEMDNESEDI
                     QHHDINNNKAIQNDTPNQKVDDKNDKNAVNKEEKHDNRVNKSAEIKVK"
     gene            2655..2996
                     /locus_tag="NBI42_002197"
     CDS             2655..2996
                     /locus_tag="NBI42_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HCD1778591.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIDNAEHTLFTPYM
                     METLSSLGVKPDIIDLIHKGTELEDFAAFNLSIDDTVTVCLQRTEELLKQYKNVEFND
                     KILINWHIMQK"
     gene            3097..3669
                     /locus_tag="NBI42_002198"
     CDS             3097..3669
                     /locus_tag="NBI42_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778592.1"
                     /translation="MEKNEYTAKYNEYSQLLDATYSQAVAYLLNKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISEFKYPYNYQKKENL
                     VYCDLIEHLILHAIITKESNGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPGILVEKLLIKIDDMLKGIEIYDFLESR"
     gene            complement(3867..4424)
                     /locus_tag="NBI42_002199"
     CDS             complement(3867..4424)
                     /locus_tag="NBI42_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCD1778593.1"
                     /translation="MKVIEKVHYEDILEPKIVRLFQPLVKNKMDQLFITSEHFAIDID
                     KLLFQLNLTVKYIKMEGNTSGYLSGRTIYINNNHSINNTRFAIARELGHYLYKYKDNN
                     DNSINNLREITYESDVNQFSVDLLMPKKQIEALVYNFYEVNNINLSSGLSEKERNKLL
                     NLISSKLEVSKIAAGLRLYNLGIHI"
     gene            complement(4610..4795)
                     /locus_tag="NBI42_002200"
     CDS             complement(4610..4795)
                     /locus_tag="NBI42_002200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778594.1"
                     /translation="MKQISIGYEEIYGDRIFHSHFLKEQEVKNWKTVNQIIEKEHLDK
                     NEYEAEPPLFFFSGSLD"
     gene            complement(4797..4973)
                     /locus_tag="NBI42_002201"
     CDS             complement(4797..4973)
                     /locus_tag="NBI42_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778595.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSAIFAVL
                     LMLLVYIFLGILKK"
     gene            complement(4984..5367)
                     /locus_tag="NBI42_002202"
     CDS             complement(4984..5367)
                     /locus_tag="NBI42_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778596.1"
                     /translation="MINQNLFDELEKKGCKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     IYIKLKTKKNAAEEYVLTGEKWGYQ"
     gene            complement(5815..6051)
                     /locus_tag="NBI42_002203"
     CDS             complement(5815..6051)
                     /locus_tag="NBI42_002203"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD1778597.1"
                     /translation="MVRLYENGKPRNEIIREYDLTPLTLGKWIKQHQKSGSFNHQDNL
                     TDEEKELIKLRKEVQHLKMENGILKQAALIMGQK"
     gene            6196..6291
                     /gene="pepA1"
                     /locus_tag="NBI42_002204"
     CDS             6196..6291
                     /gene="pepA1"
                     /locus_tag="NBI42_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HCD1778598.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            6414..6563
                     /locus_tag="NBI42_002205"
     CDS             6414..6563
                     /locus_tag="NBI42_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778599.1"
                     /translation="MQGFKEKHQELKSALCQIVLIRSISEVRQLNIATYSNLGNGRNR
                     IPTFR"
     gene            6508..6663
                     /locus_tag="NBI42_002206"
     CDS             6508..6663
                     /locus_tag="NBI42_002206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778600.1"
                     /translation="MRHIATWEMEEIEYLHLDDEDKEDILYFEKEDDKVIAVTNILWT
                     GSGFNDI"
BASE COUNT         2611 a         1128 c         1042 g         2271 t
ORIGIN      
        1 agttgcaata caactttaca attattaaat taacacttct acaacccaaa aatattttta
       61 aagttttgaa aaaaattaaa gtatttccac tactaatttg gtaaaatatg attgtgtgat
      121 atagtgaaag cgttttacac ttaataattc cctcttaaat acatccaggt tttatgtagt
      181 aaatcatgaa aataacatat aaatatagag gagatttacc tttgaataca gagaacaaca
      241 agaatcaaaa ccaatctgct aaaaattctg aaagacgtgg catgttaaaa ggatgcggcg
      301 gttgccttat ttcttttatt ttattaatta tcttattatc agcctgttca atgatgttta
      361 gtaataatga caattccact actaatcaat catcaaaaac gcaattaact caaaaagatg
      421 aaaataaaaa tgaagataag cctgaagaaa aatcagaaac aaaaacagat gaggatttac
      481 aatcaaccga agaagtaccc gcaaatgaaa atactgaaaa taatcaacat gaaattgatg
      541 aaataacaac aacagatcaa tcagatgatg aaattaacac accaaacgtt gcagaagata
      601 aatcacaaga tgacttgaaa gatgatttaa atgaaaagca acaaccaagt gaccatcatc
      661 aatccactca acctaagact tcaccatcaa ctgaaacaaa caagcaacaa tcatttgcta
      721 attgtaagca acttagacaa gtatatccga atggtgtcac tgccgatcat ccagcatatc
      781 gaccacattt agatagagat aaagataaac gtgcatgtga acctgataaa tattaaacaa
      841 caagcgaatt gaattcaaat tgtatttagc tttatgcact aatcacatag taaataatga
      901 gggagatttt ttaggcatga gcaatcaatt caaaagcgaa gaagagcgaa gacaatggga
      961 acaattccaa gctttccaaa atcaacaaaa ccaacagcac cagcaatacg gacaaaagaa
     1021 atctaaaaaa ggatggttct ggggctgtgg tggttgtcta gtattattta ttttaattat
     1081 catcggtatt tcagcttgta cagctggtat tacaggtaac cttggcggaa atagttctaa
     1141 agaaacgaac aaaacccata aaatcggtga aactgttaaa aatggcgacc ttgaagtcac
     1201 tgtaaattca gtggaaacta tgaaatctgt aggaccatct cttgcaccaa caaacgctaa
     1261 aggtatattt gtcgttgctg atgtgacgat taaaaacaaa ggtaaagaag cgttaacaat
     1321 tgatagttca atgtttaagc taaaatccgg tgataaaaca tttgaagcag ataatacagg
     1381 ttcaatgtct gctaatcaaa gtgacaatgg tagtatagaa aattcatttt tcttacagcg
     1441 tataaatcct gatagcactg ctcaaggtaa aattgttttc gatgtgtcag aaaacatagc
     1501 caacgcaaaa gataaaaaat tagaagttat ttctagttta tttagcgtca agaagattac
     1561 atttgattta tccgatgcta aaaaaacatc aaaagctaaa aaagacaagc aagatacaga
     1621 agtagctgtt gcgagttcaa atagcgataa tgtaaattat gaagcttcgg ctactacacc
     1681 tgctacaact tctagtgcgg atactgattc tgaagatagc gaaaagtcta gtaaagatga
     1741 ggataagcag aattcgtcta aaagtgataa atctagtgta gaaaaaagtg aatctaatga
     1801 ggaaactgct cctgtagagc ccatgcccca gagcaaacct accactagtg aagcatcacc
     1861 tagtcaaaat attcacaatg aagatagcat gtacgacgct tcaacagaat aaaatttcag
     1921 ggtagctcag ctacccttct tttacggaaa aattaattat acataatcaa attaaggaga
     1981 taaaaaaatg aaattcaaag ctatcgttgc aatcacatta tcattgtcac tattaactgc
     2041 ctgtggtgct aatcaacata aagaaaatag tagtaaatca aatgacacta ataaaaagac
     2101 gcaacaaact gacaacacta cacagtcaaa tacagaaaag caaatgacac cacaagaagc
     2161 cgaagatatc gttcgaaacg attacaaagc aagaggtgct aatgaaaatc aaactttaaa
     2221 ttataaaaca aatcttgaac gaagtaatga acatgaatat tatgttgaac atttagtccg
     2281 cgatgcagtt ggtacacctt taaaacgttg cgctattgtt aatcgacaca atggtacgat
     2341 tattaatatt tttgatgata tgtcagaaaa agataaagaa gaatttgaag catttaaaaa
     2401 gagaagccct aaatacaatc caggtatgag tgatcaagct gaaatggata atgagtcgga
     2461 agacattcaa catcatgata ttaacaataa caaagccatt caaaatgata caccaaatca
     2521 aaaagtcgat gataaaaacg ataaaaatgc tgttaataaa gaagaaaaac acgataaccg
     2581 tgtaaataaa tcagcagaaa ttaaagttaa ataagggtat actttgatta atcgtaattt
     2641 ttaggagaac cgctatgata acatttgaaa atatacaaca acttgaaaaa tacaccttaa
     2701 tgactatgca tggtcttttt aatcaactta aactcggtat tatttcaatc gacaatgcag
     2761 agcatacgct ctttacacct tatatgatgg aaacactctc ttccctaggt gtgaaaccag
     2821 acattatcga tttaattcac aaagggacag agttagaaga ctttgcagca tttaatttat
     2881 caattgatga tacagttaca gtctgtttac aaagaactga agaactatta aaacaataca
     2941 aaaatgtaga attcaatgac aaaatattaa tcaattggca cattatgcaa aagtaataga
     3001 catataaaag tcgaacataa ctaagtgggt attgatttta ttatttctaa gttacaagta
     3061 tttcatttta taaagtttga aaagaggttt attgaaatgg agaaaaacga atatacagct
     3121 aaatataatg aatatagtca attattagac gctacttact cgcaagctgt agcatacctt
     3181 ttaaataaat atggcgctgt aaccgatgac tattataagg aaaaatcata cacgcgattt
     3241 ttaaatggag aaatcaaaag tatttcaaaa ggaaaataca ctagagctag cgaaggatta
     3301 tattgtcatc acataagcga agacaaattc caaaacttat ctgacctaag attcatttcc
     3361 gaatttaagt acccatacaa ctatcaaaag aaagaaaact tagtttactg cgatttaatc
     3421 gagcatttaa ttttacatgc aattattaca aaagaatcca atggccaatt tggcgtggct
     3481 ggattatgtc aaatgatcaa accaacagtc attgattggt acattggcga atataatcca
     3541 aaaccagcat ggatgcaagc caccaaagca cgtgcctatc tgcctggaat attagtagag
     3601 aaattactca ttaaaattga cgatatgtta aaaggaatag aaatatatga tttccttgag
     3661 tctagataaa tgattaatgg agatttattt tttgctgttg agattttgtt atagatattt
     3721 aaacctgtaa ttaaatatat tttataaaaa tagaccacgc atacctatct ataaacggac
     3781 aatgtttata aatgagtttg cctggtcttg aattgtatta gttttctttt ggttttaata
     3841 aatcacttag cttattacat tatttattaa atatgtattc ctaaattata cagtcttaat
     3901 ccagcggcta ttttcgaaac ttccaattta gatgatataa gatttaatag tttgtttcgt
     3961 tctttttcag ataaaccaga acttaagttg atattattga cttcataaaa attatagact
     4021 aatgcctcta tttgcttttt aggcataagt aagtcgactg aaaattgatt tacgtcgctt
     4081 tcataagtga tttctcgtaa attatttata ctattatcgt tgttatcttt atatttgtat
     4141 aaataatggc cgagctcacg agctattgca aatcttgtat tattaatcga gtgattatta
     4201 ttgatataaa tcgttcttcc acttaaataa cccgaagtat taccctccat tttaatatat
     4261 ttaactgtta aattaagttg aaataatagt ttatctatgt caatagcaaa gtgttcagaa
     4321 gtaataaaaa gttgatccat tttgttcttt accaatggct gaaataatcg aactattttt
     4381 ggttctaaaa tatcttcata atgaactttc tcaataactt tcatttgatt ccccacccta
     4441 actaaaaaat taaacctaca tcatttaata aatttataac tataggtgca aacattatta
     4501 aaataaaacc cctccactac taaatgtaac agagggacat gattccaaaa taaaatgtca
     4561 ttttttataa atttattata atatttttca tcatactcta caaccgaatc taatccaacg
     4621 atcccgaaaa gaagaacaac ggtggctcgg cttcatattc atttttgtcc aagtgttctt
     4681 tttcaattat ttgattgacg gttttccaat tttttacttc ttgctctttt aaaaaatggc
     4741 tatgaaaaat tctatcccca tagatttctt catagccaat gctaatttgt ttcatgtcat
     4801 ttctttaaaa ttcctaaaaa tatataaact aaaagcataa gtaacacagc aaaaattgcc
     4861 gaatatataa ttattgaaaa agcattgctt tgcattggaa ctaaataatt taaagtacaa
     4921 aataataata atgctaaaat tccaacttta agtgaatatt ttaatctatc cataaaatca
     4981 cccttactga tatccccatt tttctcctgt cagaacgtat tcctcggctg cattcttttt
     5041 agtttttaat tttatatata ttcccttact agtatcaata ttcgatgcag ctatagatcc
     5101 aaggaaacca ccaacacctg cgccaactaa acctcctgtt agtgcagaaa taacgctaat
     5161 agcccctgca cctaaagcag ctattgtttt tgtatatgca gaagaaagat ataatgttgc
     5221 agtatcttta cctgtttcta catattgagt tttacccgct ctcaattggt cttcagcttt
     5281 atattttttt atttcttctt ttgtaaatat atcttccagt ttacaacctt ttttctcaag
     5341 ttcatcaaat aaattttggt taatcaaata tattaccttt gcttgagaat ggtctaactt
     5401 atcttcagca tgagctacat ctcaattata agataatgaa attggactaa caaataatac
     5461 accagcagct aataataatg attttaattc gtttttcatt agtttctttt ggatgatttt
     5521 tgtatttagc tttcgtataa acagaaacta gattacactt tttcatgata cgacctatct
     5581 ttcgtccaga tatagaaaga tccttgtcat ttaaactatt tttaattcat cttgtagcaa
     5641 agacttttct attagaatta aaaatattta tgacggcacg ttctacgttt gaatcacctt
     5701 tagtgatttt attatctttt ctttttatag aatcataata ggtacttctt agtattttta
     5761 ggactttaca cattgctgat actgaatatt gatgtgcatt cttttgaatg acttttattt
     5821 ttgccccata atcagcgctg cttgctttaa aataccgttc tccattttta aatgttgaac
     5881 ttctttgcgt aatttaatca gctctttttc ttcatctgtt aagttatctt gatgattgaa
     5941 tgaacctgat ttttgatgtt gctttatcca ttttcctaac gtcaaaggtg ttaaatcata
     6001 ctcgcgtata atttcatttc ttggcttacc attttcatat aatctaacca tttataactt
     6061 aaactctgaa ctaaatgttc ttctttctta aattaacaaa atctactctc atagaatttg
     6121 tccaattaga tgatataata gtagagtcgc ctatctctca ggcgtcaatt taggcgcaga
     6181 gaggaggtgt ataaggtgat gcttattttc gttcacatca tagcaccagt catcagtggc
     6241 tgtgccattg cgtttttttc ttattggcta agtagacgca atacaaaata ggtgacatat
     6301 agccgcacca ataaaaatcc cctcactacc gcaaatagtg aggggattgg tgtataagta
     6361 aatacttatt ttcgttattt caattatact gctaattttt ctttttgtaa aacatgcaag
     6421 gttttaaaga gaaacatcaa gaactaaaga gtgctctatg tcaaattgta ctgatacgtt
     6481 caatatcgga agttaggcaa ctaaacattg cgacatatag caacttggga aatggaagaa
     6541 atagaatacc tacatttaga tgatgaagat aaagaagata tactttattt tgaaaaagag
     6601 gatgataaag taatagcagt tacaaatatt ttatggactg gctctggttt caatgatatt
     6661 tgatcaactc aataatgagt cgcctagctt tattaaaaaa ctcctttaaa gggttgttta
     6721 atcttaaatg taggcgattt tattaatgct tgaagaaaga gaatttatcc tcttaaaatt
     6781 gaattcgtca aattaaaata caagatattt gagaaactat agagtaccag tctaagtaaa
     6841 ataattccca aaaaagctga ttcattttaa tagactagtc atctgtaaag aaagttgtcc
     6901 ctaattactg ttcaaagtta tccagtgcta aagttcttgc tattttttat accgcctgtc
     6961 ggaaatttcc taaatctatt tcataagact acatctgata tatgatcaaa agagtttatc
     7021 atattaaatt tatagattgt tctgtttatc tg
//
LOCUS       DAHHRR010000119         7042 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.119, whole genome shotgun sequence.
ACCESSION   DAHHRR010000119 DAHHRR010000000
VERSION     DAHHRR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.119"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..193)
                     /locus_tag="NBI42_002207"
     CDS             complement(<1..193)
                     /locus_tag="NBI42_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1778601.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQ"
     gene            complement(206..745)
                     /locus_tag="NBI42_002208"
     CDS             complement(206..745)
                     /locus_tag="NBI42_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1778602.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            complement(889..1164)
                     /locus_tag="NBI42_002209"
     CDS             complement(889..1164)
                     /locus_tag="NBI42_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCD1778603.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMDAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTGKK"
     gene            complement(1332..2738)
                     /gene="lpdA"
                     /locus_tag="NBI42_002210"
     CDS             complement(1332..2738)
                     /gene="lpdA"
                     /locus_tag="NBI42_002210"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCD1778604.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            complement(2742..4034)
                     /locus_tag="NBI42_002211"
     CDS             complement(2742..4034)
                     /locus_tag="NBI42_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCD1778605.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESTASATNEEVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            complement(4125..5102)
                     /locus_tag="NBI42_002212"
     CDS             complement(4125..5102)
                     /locus_tag="NBI42_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCD1778606.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            complement(5106..6218)
                     /gene="pdhA"
                     /locus_tag="NBI42_002213"
     CDS             complement(5106..6218)
                     /gene="pdhA"
                     /locus_tag="NBI42_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCD1778607.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            complement(6389..7015)
                     /locus_tag="NBI42_002214"
     CDS             complement(6389..7015)
                     /locus_tag="NBI42_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HCD1778608.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHGEYSKAYKNAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
BASE COUNT         2008 a         1416 c         1039 g         2579 t
ORIGIN      
        1 tttgataaat cacttcacca ctgtcaggat attcaaaccc tgcaattaat tttaaaattg
       61 ttgttttacc acaacctgaa ggacctaata atgtataaaa gtatcctgat tcaatatcaa
      121 tatctatgtc atctaagata ttaagatcat catagctttt actaactgat tttaatgata
      181 ataacggttc caaataagat cccccctata aatatgaagc tgtcgctaca ataagtattc
      241 gtgtttcatt gtttgattca tttgacaaac gatgattatc tcgtgcttta aaatacaaaa
      301 cgtcttcttc ttgtgcttga tatatctctt tgcctaaatt aagtgttatc tgacctgaca
      361 tacaataaat aaacgtatca gactctgatg gattaaaatt tttatatgat gcgccaggct
      421 ttaaagttaa tattaatggc tccatatcat attcatttga ctttgaaact aaccaattta
      481 atatataacc ttcatcatac tcatcataaa taacttgttc ttccttcttg tataatactt
      541 tttcattttc actgtcttta aaaaattcac ttggcgtcgt tcctaacacc tctataatat
      601 ttaagaaagt ttccatactt ggtgaggcat gttcactttc tatttgtgaa atgtagcctt
      661 tcgataagtc tgtacgttca gcaagttctt cttgcgttaa atttttaatt cttctaagat
      721 ttttaatttt attacctatg ttcatttatt tttccaccta aaaaagctat attaagttta
      781 cttttatcaa acattttgtt taacacttga taaaggtatc aaaatatagc tttattagca
      841 atagttttaa agaaaaaatc ctattaaatt gtgttaattt cgaacgaatt attttttacc
      901 tgttactctt tgttcttcag agtgagtttt tacatcttga actgctttgt aactattata
      961 gccactactt ttttcaaaag tattaaaaat ttgtttttcc tctgctttag caggcacaat
     1021 ttctttaaat cttttatatg catccataaa gtcgcctgcc gtcacgccgg attcataata
     1081 cttctctaca tgattaaaga aatttatcac tgaaatcatc tcttcattac tccagtctaa
     1141 atcaattgga tactcatatt ccattgttga accctccatt tcaattcttt tgttagcaac
     1201 gctagtataa catgcatgtc acatgttaac gatatctcaa atcaatttcc ttacactact
     1261 ttacctaaaa caaaaaagcc gtagcatatg ctacagcttt taatgactga atctttatag
     1321 ataatcagtt attacattgt atggattggg tatccgatag ctttttccgc tgcttccata
     1381 gtcatctcac ctaatgttgg atgtgcatgg attgttaatg cgatatcttc agcattcata
     1441 ccagcttcaa ttgctaaacc taattcagag ataatatctg atgcaccagt accaactact
     1501 tgagcaccga ttaaagtatc atcttcttta agtgtaataa gtttaacaaa tccgttagta
     1561 tcatctaatg ataatgcacg accatttgct gcatatggga atttagaagc tttaattgct
     1621 aaaccttctt ctttagcttg cgcttctgaa taaccaactg tagctaattc tggttcagta
     1681 aagcatactg ctggcatacc aatgtaatca acttcagcag cttgaccatc aattgcttca
     1741 gcagcaactt tagcttcata gctagcttta tgagcaagtg gtaaacctgg aacgatatca
     1801 ccaattgcat agatattgct gatagacgta cggctttgtt tatcaacttc taataatcca
     1861 cggtcagcga atttaacacc taattcttct aggcctaatt cgtctgtgtt tggacgacga
     1921 cctacagtta ctaatacata atcagcttcg attgttttct cttcgccttt agcttcataa
     1981 gtaactttaa ctccgttatc tgtttcttca gctgatttag ccatagcttc agtaacgatt
     2041 tcaacacctt tttctttcat acctttttta acaggttgtg tcatttgttt ttcgaagcca
     2101 cctaagatat ctttagcacc ttcaaggatt gttacttctg aaccaaagtt agcaaatgct
     2161 gtacctaatt ctgatccaat gtatcctcca ccaactacaa ctaatttacc tggtacttct
     2221 tgtaagttta aagcacctgt tgagtcgata acacgtttac cgaatttgaa attaggaatt
     2281 tcaattggtc ttgaacctgt tgcaatgatt gcatttttaa agttgtatgt ttgtgcgctc
     2341 ttttcgtcca taacacgtaa gctattatta tctacgaaat atgcttcacc tttaacgatg
     2401 ttaactttgt tacctttaag taagccttca acaccaccag ttaatttatt aacaactgat
     2461 gatttgaatt cttgaacttt ttggaagttt aaagaaacac tttcagcaat aacacctaag
     2521 ttttcagaat gttgtgcttc aacaaaacgg tgagaagcat gtagtaatgc ttttgaagga
     2581 atacatccta cgtttaagca aacaccacca agattacctt tctcaacgat tgttactttt
     2641 tgtcctaatt gagctgcacg aattgctgca acgtatccac caggacctgc tccgattact
     2701 atagtatctg tttcaattgg gaaatctcca actaccatgt tttacccctc cattaataat
     2761 aattctggat tatttaataa acgtttaatg tgattcattg cattttggcc agttgcacca
     2821 tcaatttgtc tgtggtcaaa gcttaatgat aatgctaata ctggtgctgc aacaatttct
     2881 ccatctttaa cgataggttt ttgagcaata cggccaattc ctaagattgc tacttctggg
     2941 tgattgataa ctggagtgaa ccattgtcca ccagctgaac cgatattact gattgtgcat
     3001 gttgcacctt tcatttcatc ggctgttaat ttaccatcac gtgctttaac agctaattcg
     3061 ttaatttcat cagaaatttg gaaaatagat ttacggtcag catgtttcac tacaggtact
     3121 aataaacctc tatcagtgtc cgccgcaata ccgatattcc agtaatgttt atgcacaatt
     3181 tcaccagctt cttcattgaa tgaagtatta agtgctgggt attttttcaa tgcagaaaca
     3241 agtgctttaa caacatatgg taagaatgtt aacttagtac cttgttcagc tgcgatttct
     3301 ttaaatttct tacggtgatc ccataatgct tgaacatcaa tttcatccat taatgttaca
     3361 tgaggtgcag tatgcttaga gttaaccatt gctttcgcaa ttgctctacg catagcaggg
     3421 attttttcag ttgtttctgg gaagtcgcct tctaatgata ctgctgcagg tgctgcagga
     3481 gtttcagcaa cttcttcatt tgtagctgaa gcagttgatt catttgaagc tgttggtgca
     3541 ccaccattta agtatgcatc tacatcttct tttgtaatac gaccattttt accagatcca
     3601 gaaactgctt taatgttaac acctttttca cgtgcgtatt tacgtactga aggcattgct
     3661 tttacagttc tgttttcatc tacttcttca gtttgagtag ctacaggtgc ttgctctgct
     3721 ggcgcttcct ctttcgcagg ttcttcttta gatgatgaat catcatcatg acctttaaat
     3781 tgcatatctt ctgcatcagg tgcatcgatt ttaacaataa cgtcaccaac tacagctact
     3841 gtgccttctt ctaccataac ttcttctaca gtaccagata ctggtgaagg gatttctact
     3901 actgatttat cgttttgtac ctcagctaaa acatcgtctt cttcaatagt atctccagct
     3961 ttaacaaacc attttacaat ttcaccttcg tggatacctt ccccgatatc gggtaatcta
     4021 aattcaaatg ccacgttttt gccctcctaa gatttcgtaa attaaacatt tcattttaat
     4081 caatgagact aaaatacgct aacttcgtta acttttaaaa tgtattaaaa ttctaaagtt
     4141 tcttttgctt tttcgatgat gtcatttttg tttggtaacc aaacattttc agcttgagtg
     4201 aatggataaa ttgtatctgc tgctgcaact cttccaatag gtgcttctaa tgaaaggatt
     4261 gcacgttcac ttaattcagc tacaactgct gcaccaacac cagcctgacg ttgtgcttct
     4321 tgaactacaa ctgcacgacc agttttttca actgaagcta caattgtgtc aacatcgatt
     4381 ggttgaacag tacgtaagtc aattacttca acagaataac catctttttc aagttcttct
     4441 gcagctttca ttgattcttg aaccattgca ccgtatgtga tgattgaaat gtcattacct
     4501 tcttttttca cattagcctt accaatgtca attgtatatt cttcttcagg tacttcttca
     4561 cggaatgaac gatacaattt catatgctct aagtatacga ctgggtcatt acttctaata
     4621 gaagaaatta ataaaccttt cgcatcatat gggcctgaag gaataacaac ctttagacct
     4681 ggagattgag ctaaaatacc ttctaaatta tctgcgtgca attcaggtgt gtgtacgcca
     4741 ccaccaaatg ggctacgaat tgttacaggt gcagttttag taccgcctga acggaaacga
     4801 gtacgtgcaa tttgtccagc aatcgcatca aatacttcga atacgaaacc taagaattgt
     4861 acttccataa ccggacggaa tccttcaact gcaagaccca ttgctaaacc accaatacct
     4921 gattcagcta aaggtgtatc gaatactcta tcttcaccaa attctttttg tagtccttca
     4981 gtaacacgga aaacaccgcc gttaacacca acgtcttcac caaaaattaa aacatcttgg
     5041 tcatttttaa gttcagtttt aagcgcatca ttaatcgctt gaaccattgt catttgtgcc
     5101 atggcttact tcgactcctt ctctttgtaa atttcatatt gttctgctaa gttttgaggc
     5161 atatcttcat acataatttc cattagagaa gtaacagttt gtttttcagt gttatcagcc
     5221 tctttaattg ctgctttaat atcagctttt gcacgttcaa taacttcatt ttctttgtct
     5281 tcattccata aacctttgtt ttcaaggaat ttacggaaac gcactaatgg gtcttttttc
     5341 tcccattcag catcttcgtc tgaagttctg taacgagttg gatcgtcacc agccattgta
     5401 tgaggaccat aacgatatgt catagtttca attaatgttg gaccttcacc tgcaactgcg
     5461 cggtcacgtg cttctttagt tgcttgatat acagctaacg catccatacc atcaacttgg
     5521 ataccaggaa tacctacagc aattgctttt tgagctaatg tttcagcagc agtttgtttg
     5581 cttcttggtg ttgaaattgc atagttattg ttttgaataa cgaaaattgc aggtgcttta
     5641 taagctgctg caaagttaat accttcgtag aaatcacctt gtgaagaacc accgtcacca
     5701 gtgtaagtga ttgcaactgc atttttacca cgttttttaa gtgcaaatgc aacaccagca
     5761 gcttgaatgt attgtgcacc gataataatt tgtgggctta atgcattaac gccttcaggg
     5821 aattgatttc ctttgaagtg acctcttgag aataagaaag cttcagttaa tggtaaacca
     5881 tgccaaataa tttgaggaac atctctgtat cccggtaaaa tgtaatcttc tttttctaaa
     5941 gcgtattgtg acgctaattg tgatgcttct tgaccagcag taggtgcata gaaacctaaa
     6001 cgtccttgtc tgtttaatga gatagaacgt tgatcaagga tacgagtcca taccattctt
     6061 tccattaatt ctactaattg ttcatccgta agatcaggta ctaagtcttc atttacgacg
     6121 ttaccattct catccaaaat ttgaaccatt tcaaatttcg attgagtatc atttaaaact
     6181 tttactgcat cgaattgggc ttgtaactta ggagccattc aattcaccat acctttccca
     6241 taattgtata gaaattcatt ttatccttat taattgtacc acaaataaat gggactgtta
     6301 aaccatttga aaaagttctg ttttaataaa aaaagataca gataatagga actgtattag
     6361 ttgtttccta ccatctgtat catattaatt atttaaattg atcaacgtct tgcttttctt
     6421 taccaacctt atttagtact ttcgtatact tatctgatac ttcttttaac tttttatagt
     6481 tctgttctat tgcttttgat ttttcattaa caccttgttg tgtcgcgtca ttttgattta
     6541 aatatttaaa caatgttttt tctgaattga cagcattttt ataagcttta gagtattcac
     6601 catgcaattt gtatttttct tttaatacat catctaattg ttttacttct ttacgtttaa
     6661 catcattatc aatgttatca acgtgacttt ttgctttctt aaagtcttgt tcagacttct
     6721 taattgcgtc ttcttctttt tcaaattcct ttagacgatc atcggcattt ttaattaaat
     6781 cttttactgc tttaccgcga actgctgtat ctttactatt gacatcttca gttaattttt
     6841 tctttttctc atctaattca gcaattttct tgcccacagt tttaattggt tcttcatcgt
     6901 ctttaatttt atccacttgc tttaaatatg ccttaatttc ttttttatcc gtagtacatc
     6961 ctgcaagcaa gacactagat gctaatacta ctgcgattgt ttttccaaat ttcattactt
     7021 gtacctccac ttgctattta aa
//
LOCUS       DAHHRR010000120         6992 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.120, whole genome shotgun sequence.
ACCESSION   DAHHRR010000120 DAHHRR010000000
VERSION     DAHHRR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.120"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(230..1927)
                     /locus_tag="NBI42_002215"
     CDS             complement(230..1927)
                     /locus_tag="NBI42_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCD1778609.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVGENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKMNPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVNRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDTRGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKEQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKASNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYEHIQKMKQS"
     gene            2202..2825
                     /gene="gmk"
                     /locus_tag="NBI42_002216"
     CDS             2202..2825
                     /gene="gmk"
                     /locus_tag="NBI42_002216"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCD1778610.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLDHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            2825..3043
                     /locus_tag="NBI42_002217"
     CDS             2825..3043
                     /locus_tag="NBI42_002217"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCD1778611.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            3259..4458
                     /gene="coaBC"
                     /locus_tag="NBI42_002218"
     CDS             3259..4458
                     /gene="coaBC"
                     /locus_tag="NBI42_002218"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCD1778612.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            4458..6866
                     /gene="priA"
                     /locus_tag="NBI42_002219"
     CDS             4458..6866
                     /gene="priA"
                     /locus_tag="NBI42_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCD1778613.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIEVPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQHLTEDQFKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMEASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         2430 a         1023 c         1293 g         2246 t
ORIGIN      
        1 acttttccat cagtccgatt tattatagaa ccataaatca atagtagttc tttattattt
       61 ttatttcact catttttaaa atcacaaaga taatttgtaa cataaatgat gatgcgttat
      121 taaaaaacac gatacttcgt ttcaatgaac gcattaaatt aaaaataaac acctcgcaaa
      181 agaaagtagt cctttcttat ggtgaggtgt ttatttgtcg acaacttgat tatgattgtt
      241 tcattttttg aatatgttca taatcaggtg tagcatacaa agttttttga ttgtcatatg
      301 ttacaaaccc aggctttgca cctgatggtt tgtgcacatt tttaattaac gtgtaatcaa
      361 caggtatttg tccagaatta ctagcttttg aaaaatatcc tgctaacata gccgcttcct
      421 tgattgtcgt atcacttggt gcatcattaa atataacgac atgtgaacca ggaatatctt
      481 ttgtgtgtaa ccatgtgtga gtttttttag cttttttatt tgttaaataa tcattttgct
      541 tgttattctt gccaacatat atatcgtcgc catcagttga tacataatgt tgtaattgaa
      601 tctgctcttt ctttttctta gtttgatttt tacgctgttt cataaagcct tgttctgcta
      661 attcatctct aatttcatca atgtcatgga cagaaatatg atgtaattgt tgttcgattg
      721 ttgaaaaata atctatattg tctttcgtca attgaatttg atgttgtaat tcacgttctc
      781 tcgttttcat acgattatat tgtttataat aatattgagc atttgcagat ggggattttg
      841 taggatttaa aggaatgaca acttcttcat tcgtataata attcaatgcc gtcacttctt
      901 tatcgccttg cttaattcga tatatattag cagtgatcaa ttcaccatat aactgttcag
      961 tatctttatt tttagactgc tcatattctt caatcaactt cgctaattta ttttgatatt
     1021 tgtgcaactg ctgttgaaca aatcgaacta aatcattcgc acgttgttta acgcgttcac
     1081 gttcgccacg cgtatcataa aaacgatcaa gtaaatcatt taatgaatcg tacgtaactg
     1141 tatcatcatt aaattgattt aactttataa aatagaaatc ctctttacct gtttcatgat
     1201 ttttatgaaa aataggagta ggtggtaact tggtttctgc cattacttcg tcgaatgctt
     1261 ctggtaatgt tgatgaagtc ataaattgac gacgattaac gatttcattc gtaattaaag
     1321 ggctaaatcc ttcaaactga ttcaataatt gtttagcaat attacctgcg ttaaaatcga
     1381 tatatttcaa cacctctgca cctgtaatat catacggatt cattttgtgc tgagtaggtg
     1441 gtgcttcata attaaaacct ggcattactg tacgatagtg attcgtattt ggtgttaagt
     1501 gtttaaatcc ttcaattatt ttgcgatttt cacctactaa aattaagtta ctatgtttac
     1561 ccataatctc aaggatgaca gttcggtaaa tagtatcgcc aatttcatct ttactcttta
     1621 tatcgatttc aatgcgacga tcattaccaa tttgcttaat cgattcgata ataccacctt
     1681 ctaagtgttt tctaaaaaca cgcgcaaaca tgggtggatt aaatggatta tcatattttt
     1741 ttgtagtcaa ttgtaatctt gaaaagtttg gatggattga caataacaat tgatggtttt
     1801 gtctattttg acgtacaacc attagtatcg tgtcattatc aggttgattg attttgtgaa
     1861 cgcgtcctgt tgttaaaaat tgtagagact caaccatttt ctttgtaaat aagccatcat
     1921 aagccataat ttatcttcca ccttcttcat atcattcgtt tcattttaac atgattgtaa
     1981 cttttcggta tagttattga atcatacatt atgccacaca ttttacatta tctaaaagtt
     2041 cgcaattcaa tttatttaat taaaattaat cgtgcaatat aacgatattg ttagacctaa
     2101 tagaaattat ggcatgcaat ttcaaatatg ctatacaata taaagaacaa tgtgatatca
     2161 tatttaaata atagaagatt agcttagaga ggtcgtaagg catggataat gaaaaaggat
     2221 tgttaatcgt tttatcagga ccatctggag taggtaaagg tactgttaga aaacgaatat
     2281 ttgaagatcc aagtacatca tataagtatt ctatttcaat gacaacacgt caaatgcgtg
     2341 aaggtgaagt tgatggcgta gattactttt ttaaaactag ggatgcgttt gaagctttaa
     2401 ttaaagatga ccaatttata gaatatgctg aatatgtagg caactattat ggtacaccag
     2461 ttcaatatgt taaagataca atggacgaag gtcatgatgt atttttagaa attgaagtag
     2521 aaggtgcaaa gcaagttaga aagaaatttc cagatgcgtt atttattttc ttagcacctc
     2581 caagtttaga tcacttgaga gagcgattag taggtagagg aacagaatct gatgagaaaa
     2641 tacaaagtcg tattaacgaa gcacgtaaag aagtcgaaat gatgaattta tacgattacg
     2701 ttgtagttaa tgatgaagta gaacttgcga agaatagaat tcaatgtatt gtagaagctg
     2761 agcacttaaa aagagagcgc gtagaagcta agtatagaaa aatgattttg gaggctaaaa
     2821 aataatgtta aatccaccat taaaccaatt aacgtcacaa attaaatcaa agtatttaat
     2881 tgcaacaact gcagcgaaaa gagcgcgtga aattgatgaa caacctgaaa ctgaattatt
     2941 aagtgaatat cattcattta aaccagttgg tagagcgtta gaagaaattg ctgacggtaa
     3001 aattcgccct gttatttcaa gcgattatta tggtaaagaa tagtttcgac cattaaaaat
     3061 atgtgaagca agacgtacat caaatacgtc aaggcatatc caaatcaaac gcacctatgt
     3121 aggtgtgttt ttttagttta tccaatgatt gatgttataa taatactaaa tttgtatcta
     3181 taaaaaagta atgagcattt gtgcgcatat gatgatgtaa agcgtaaatg tcatagtgat
     3241 atttaaggga gattgaatat gaagaaaata ttgttagccg ttacaggtgg cattgcggca
     3301 tataaagcga ttgatttgac aagtaagtta acacaatctg ggtatgaagt tcgcgttatg
     3361 ttaacgaatc acgcacaaaa atttgtgaca ccattagcat ttcaagcaat aagtcgaaat
     3421 gctgtttata cagatacttt tatagaagaa aatccttcag aaatacagca tattgcatta
     3481 ggtgattggg cagatgcaat cattgttgca cctgcaacgg caaatacaat tgcaaaattg
     3541 agtgtaggta ttgctgatga tttggtgaca tcaacgttgc tagcaacaga gacaccgaaa
     3601 tttattgcgc ctgccatgaa tgtgcatatg tatgaaaata agcgtacgca gcaaaatatt
     3661 aatattttaa aagaagatgg gtatcatttt atcgaaccag gaagcggatt tctagcatgt
     3721 ggttatgttg ctaaaggacg tatggaagaa ccgcttcaaa tcgtttctgt tattgatgct
     3781 cattttcaaa atagtaatcg tttagctaat agttcatttc aagataaacg cgcattggtt
     3841 acagccggac caactattga agttatcgat ccagtaagat ttgtatccaa tcgttcttct
     3901 ggaaaaatgg gttatgcaat agctgaagca ttgcgaaatc gaggagctat cgtgacgtta
     3961 gttgcgggtc ctacaacact agaggatcca aaagatattg aagttattca tgttcaaagt
     4021 gctgaagaaa tgtttgaaca agtgactagc cgatttgacg aacaagatat tgttgtaaaa
     4081 gcagcagccg tatctgacta tacaccggtt gatgtattag aacataagat gaaaaagcag
     4141 gatggtgatt tgtcagtatc ttttaaacgt actaaagaca ttcttaaata tttaggtgaa
     4201 cataaaacat cacagtattt aataggcttt gcagcagaga ctgaagatat tgaaaattat
     4261 gcacaacaaa aattacgcaa gaaaaatgca gatgtgatta tttcaaataa tgttggggat
     4321 atgtctatcg gatttagttc tgatgataat gaattgacaa tgcattttaa aaataatgaa
     4381 aaagtaaata tcaagaaagg aaaaaaagta gtattagctg cacaaatttt agatgaacta
     4441 gaaactaggt ggcaataatg atagcgaaag tcatagtcga tgtcgcgtcg aagagcgttg
     4501 actataaatt tgattatata attcccgaac aactcgaatc tgtcatccaa cctggtgtgc
     4561 gtgtgattgt accttttgga ccaagaacga ttcaaggtta tgtaatggaa gtaacagcag
     4621 aacctgatgc acaacttgac gtttcgaagt taaaaaaaat catagaagtg aaagatatac
     4681 aacctgaatt aacatcagaa ttaatagctt taagtgagtg gatgggttca actcatgtca
     4741 ttaaacgtat ttctatgctg gaagtaatgc ttccgagtgc tattaaagcg aagtataaaa
     4801 aagcatttaa gatgaaagat gacatagagg taccttcagc tttattacaa aaatttgata
     4861 agcatggtta ctattattat aaagatgcgc aaaaaaataa tgatattcaa ttgcttatga
     4921 agttgttaaa agatgatatc gttgaagaaa aaacgattct cacacaaaat ataactaaaa
     4981 aaaccaagcg tgctgttcgt gtcattgaag ggtatcatcc tgatgaagta ttagctaagt
     5041 tggagaaagt tattaaacaa tacgatttgt atgcttactt gtctgaagaa caacataaaa
     5101 caatattttt aactgatatt gaggatatgg gcttttcaaa atctagttta gatggactta
     5161 tcaaaaaagg ttatgttgaa aaatatgacg cggttgttga aagagaccca tttaaagatc
     5221 gtgttttcga acaagaatca aaacagcatt taacagaaga ccaatttaaa gcatatgaag
     5281 cgattaaagc taaaattgta agccaagagc aagaaacatt tttacttcat ggtgtgacgg
     5341 gatcaggtaa aacagaagta tatttacaaa cgatagaaga tgttttaagc caaggaaaac
     5401 aggcgatgat gttagttcct gaaatcgctc taacaccgca aatggtttta cgcttcaaac
     5461 gtcgatttgg tgatgacgtt gctgtattac attctggctt atctaatggg gaacgttatg
     5521 atgagtggca aaaaataagg gatggtcgtg cgagagtaag tgttggtgca aggtcaagtg
     5581 tgttcgcacc tttcaaaaat ttagggttaa tcatcattga tgaagaacat gaatctacat
     5641 ataagcaaga agattatccg agatatcacg ctagagaaat tgcccaatgg cgaagtgaat
     5701 atcatcactg cccagtcatt ttaggaagtg caacaccatg tcttgaaagt tatgcacgag
     5761 ctgaaaaagg cgtttatcat ttgctatcat taccaaacag agtgaaccaa caagctttac
     5821 ctgaaattga tatagtagac atgcgtgaag aattgagtga aggtaatcgg tcaatgtttt
     5881 caaaagattt acgtgaagcc atacaattaa gattagatcg acaggaacaa gttgttttat
     5941 ttttaaatcg acgtggttat gcatcattta tgttatgtcg agattgtgga tatgtaccgc
     6001 aatgtccaaa ctgtgatatt tcattaacgt atcataaaac gacagactta ttaaaatgtc
     6061 actattgtgg ttaccaagag acgccgccga atcaatgtcc aaattgtgag agtgaacaca
     6121 ttcgacaagt aggtacagga acacaaaaag ttgaagaact attgcaacaa gaatttgaag
     6181 atgcgcgcat aattaggatg gatgtagata caacctcaaa gaaaggtgca catgaaaagt
     6241 tattgactga attcgaaaaa ggtaacggtg acattttact aggtactcag atgattgcga
     6301 aaggattaga ttatccaaac attactttag ttggtgtgct gaatgcagat acaatgttaa
     6361 atttacctga ttttcgagcg agcgaacgta cttatcaact attaacgcaa gtggctggta
     6421 gagctggtcg tcatgaaaag gcaggtcaag tcatcattca aacgtataat ccagaccatt
     6481 attcaatatt ggatgttcaa aaaaatgatt atttaacatt ttatcgtcag gaaatggaat
     6541 atcgtaaatt aggaaagtat ccaccgtatt attatttgat taatttcaca atctcacata
     6601 aagaaatgaa gaaggttatg gaagcatcac agcatgttca taaaatttta ttacagcatt
     6661 taacagaaaa agcgcttgta ctaggtccat ctccggcagc acttgcgaga atcaacaatg
     6721 aatttagatt ccaaatttta gtgaaatata aaagtgaacc tggattatta caagccattc
     6781 aatttttaga tgactattac catgaaaaat ttataaaaga aaaattagca ttgaagattg
     6841 atattgatcc acagatgatg atgtaacatt actaattatt agaaacaagt tcagtatgta
     6901 cgagtatttg aaccagtgtt tgaatattta ctttaagtac agtaaaaggg cagaatatac
     6961 aactgttaac catttaaatt agcagtttat at
//
LOCUS       DAHHRR010000121         6972 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.121, whole genome shotgun sequence.
ACCESSION   DAHHRR010000121 DAHHRR010000000
VERSION     DAHHRR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.121"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            366..1544
                     /locus_tag="NBI42_002220"
     CDS             366..1544
                     /locus_tag="NBI42_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCD1778614.1"
                     /translation="MSKRSSVIAHYHNDQFQREFTIQNNKNGYNYLLKYLNNLDHLQI
                     IFESTGIYSRGMTRFCRVNHINYIEMNPLEAKFRTSSLRSWKTDQADAHKLAQLAPTL
                     KATETISMYEDIFFELRERARFHLEIENEQNRLKFEIVEQLHQTFPGLEKLFSSRYSV
                     IALNIAERFTHPDMVRNMDIDTLISHIFHSTDKGLSMNKAENYAHQLVNIARESYPNV
                     DRHSFLVEKARLLIRQLKQSIQHLKQLDEDMIQLAQQLDCFEHIHSIPGIGKLSAAMI
                     IGELGDITRFKSNKQLNAFVGIDIKRYQSGSTQSRDAINKRGNKKARRLLFWVVMNII
                     RGQHHFDNHVVDYYYKLRKQPHEKAHKTAVIACINRLLKTIHYLVMNQKLYDYQMSPH
                     "
     gene            2061..2993
                     /locus_tag="NBI42_002221"
     CDS             2061..2993
                     /locus_tag="NBI42_002221"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCD1778615.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            2999..3838
                     /locus_tag="NBI42_002222"
     CDS             2999..3838
                     /locus_tag="NBI42_002222"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCD1778616.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPGEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGPVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLIWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
     gene            3846..4331
                     /locus_tag="NBI42_002223"
     CDS             3846..4331
                     /locus_tag="NBI42_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1778617.1"
                     /translation="MRKFLSKTYHHTNPLWHVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQILNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEVLVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            4339..5778
                     /locus_tag="NBI42_002224"
     CDS             4339..5778
                     /locus_tag="NBI42_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD1778618.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMANQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDLMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            5845..6780
                     /gene="trxB"
                     /locus_tag="NBI42_002225"
     CDS             5845..6780
                     /gene="trxB"
                     /locus_tag="NBI42_002225"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCD1778619.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
BASE COUNT         2444 a          976 c         1324 g         2228 t
ORIGIN      
        1 cttacaacaa aacgtctcca caagtggggg ttattatcac taacccaact cgcagagacg
       61 gcttacgcaa gatattgaac cgccgagtac ggaaccgtac gctcggtggt gtgagaggac
      121 gaataatcaa gtaatgatta ttctcctact cgattagtta tagagaggag tagactgtgc
      181 gctctcttgg gtcttaaacc cgtataaaaa acaagtcagc tttactctcg ccttttgaaa
      241 ttcgtttgaa gtatgttggg ttcttgaaac cgtgtagagg aaaatgaaat gagaaaggtt
      301 aagtaaagtt ctcacttctc aattttttaa aggaggctat atttatcaat tacttaggtg
      361 ttgatattag taaaagaagt agtgtcatag ctcattatca taacgatcaa tttcaaagag
      421 aatttaccat tcaaaataat aaaaatggtt ataattattt attaaagtat ttgaataatt
      481 tagaccacct acaaatcatt tttgaatcta ctggtattta ttcaagaggt atgactcgat
      541 tttgtcgcgt aaatcatatt aattatattg agatgaaccc gttagaagct aaattcagaa
      601 caagctcttt aagatcatgg aaaaccgatc aagctgatgc acataaactt gcgcaattag
      661 cacctacttt aaaagcaacg gaaaccatat ctatgtatga agatatcttc tttgagttgc
      721 gagagcgtgc acgttttcat ctagaaattg aaaatgaaca aaatcggctt aaatttgaaa
      781 ttgttgaaca gcttcatcaa acttttcctg gtttagaaaa attatttagt agtcgatatt
      841 ctgtcattgc acttaacatc gcagaacgat ttactcatcc agatatggtg agaaatatgg
      901 atattgatac ccttatttct catatattcc attcgactga taaaggtcta tctatgaata
      961 aagctgaaaa ttacgcacac caattggtta acattgctcg ggaaagctat ccgaatgtcg
     1021 atagacattc attccttgta gaaaaagcac ggttgttaat tagacaatta aaacaatcta
     1081 tacaacatct taaacaatta gatgaagaca tgattcaatt agcgcaacaa cttgattgtt
     1141 ttgaacatat tcattcaata ccaggaattg gaaaattatc tgcagctatg attattggag
     1201 aacttggaga tattacgcga tttaaatcga ataaacaatt aaatgcattt gtaggcattg
     1261 atatcaaacg atatcaatca ggtagtacgc aaagtagaga cgcaattaat aagcgtggca
     1321 ataaaaaagc aagaagatta ttattttggg tggttatgaa tataataaga gggcagcatc
     1381 attttgacaa tcatgtcgtc gattattact ataagctaag aaagcagcct catgagaaag
     1441 ctcacaagac tgccgtcatc gcttgtatta atcgtctctt aaaaacgatt cattatttag
     1501 taatgaatca gaaattgtac gattatcaaa tgtccccaca ttaaccaacc gtacaatcaa
     1561 atacagtgta acaccttatt caaaaaaatt aaattgaacg ggctaattta gtaatgctta
     1621 ttttaaacat aaagacttga ctaatcgtag gaatgggagt gggacagaaa tgatattttc
     1681 gcaaaattta tttcgtcgtc ccaccccaac tagcattgtc tgtagaaatt gggaatccaa
     1741 tttctctgtg ttggggccca ccccaacttg cattgttggt cgaaattggt gttccaattt
     1801 ctctgtgttg gggccccgcc aactaattcg aatttatcat tgtagagctt aggtcattga
     1861 tttatggctc ggacttttat ggcgatatga accatgtaaa ttaagcaagc aataaattaa
     1921 tgattgatat tgacttgtaa aataataaca ataatgaaca attaatattt attttagctt
     1981 ttcaatgtag attggtgtta tatttttgat atgataagaa gagatgtaag agtagggata
     2041 aatacaattg aggtgaaccc atgttaacga cagaaaaact agttgaaaca ttaaagttag
     2101 atttaatcgc tggtgaagaa ggactatcga agccaattaa aaatgctgat atatcaagac
     2161 cgggcttaga gatggcaggt tatttttcac attatgcgtc agatagaata caactattag
     2221 gaacaacgga actatcgttt tacaatttat taccagataa ggatcgcgca ggtcgtatgc
     2281 gtaaactatg cagaccagaa acgcctgcaa ttattgtgac acgtggattg cagccaccag
     2341 aagaattagt tgaagctgca aaagaattaa atacgccact tatagttgct aaagatgcga
     2401 ctacaagttt aatgagtcgc ttaacaacgt ttttagagca tgcacttgca aagacgacat
     2461 ctttacatgg tgttttagta gatgtttacg gtgttggtgt attaattacc ggtgattcag
     2521 gaataggtaa aagtgagact gcgttggagt tagttaaacg tgggcataga ttagtagcag
     2581 atgataatgt agaaatacgt caaattaata aagatgaact aataggcaaa ccaccaaagt
     2641 taatagaaca tctattagaa atacgtggac taggtattat caatgttatg actttatttg
     2701 gcgcgggttc aatattaact gaaaaacgaa ttagattaaa tattaatttg gaaaactgga
     2761 acaagcaaaa gttatatgac cgcgtcggtc ttaatgaaga gacgttaagt attttagata
     2821 ctgaaatcac taaaaaaaca atacctgtaa gacctggtag aaatgttgcg gtaattattg
     2881 aggtcgctgc aatgaactat cgattaaata tcatgggcat taacactgcc gaagaattta
     2941 gtgaaagatt aaatgaagaa attatcaaga acagtcataa gagtgaggag taggttgaat
     3001 gggtattgta tttaactata tagatcctgt ggcatttaac ttaggaccac tgagtgtacg
     3061 atggtatgga attatcattg ctgtcggaat actacttggt tactttgttg cacaacgtgc
     3121 actagttaaa gcaggattac ataaagatac tttagtagat attatttttt atagtgcact
     3181 atttggattt atcgcggcac gaatctattt tgtgattttc caatggccat attacgcgga
     3241 aaatccaggt gaaattatta aaatatggca tggtggtata gcaatacatg gtggtttaat
     3301 aggtggcttt attgctggtg ttattgtatg taaagtgaaa aatttaaacc catttcaaat
     3361 tggtgatatc gttgcgccaa gtataatttt agcgcaagga attggacgct ggggtaactt
     3421 tatgaatcac gaggcacatg gtggacctgt gtcacgcgct tttttagaac aattgcattt
     3481 gcctaatttt ataatagaaa atatgtatat taacggccaa tattatcatc caacattctt
     3541 atatgaatcc atttgggatg tcgctggatt tattatctta gttaatattc gtaaacattt
     3601 aaaattagga gaaacattct ttttatattt aatttggtat tcaattggtc gattctttat
     3661 agaaggatta cgtacagata gcttaatgct cacaagtaat attagagttg cacaattagt
     3721 atcaattctt ttaattttaa taagtataag tttaattgta tatagaagga ttaagtataa
     3781 tccaccgttg tatagcaaag ttggggcgct tccatggcca acaaaaaaag tgaagtagtg
     3841 atattttgag aaaattttta tcaaaaacat atcatcatac aaacccttta tggcatgtat
     3901 accgtcttgt taaattttcg aaagttttta agaatgtaat cattattgaa ttttcgaaat
     3961 ttattccaag tatggtactg aaaagacata tatataaaca aattttaaat attaatatcg
     4021 gtaatcaatc gtcgatagct tataaagtaa tgttagatat tttttaccca gaactgatta
     4081 cgattggtag taacagtgtt attggttaca atgtaacaat tttgacgcat gaagtattag
     4141 ttgatgaatt tcgttatgga ccagtgacga tagggtctaa cactttgatt ggtgcaaatg
     4201 ctaccatttt acccggtata acgattggtg acaatgtaaa agttgcagct ggtacggttg
     4261 tttcaaaaga tataccggat aatggatttg catatggcaa ccctatgtat ataaaaatga
     4321 ttaggaggtg acaattttat ggcgcaaaag aataataatg taattccaat gacttttgat
     4381 gatgcatttt atcgtaaaat ggctaatcag aagtttaaac aaagagaata taaacgagct
     4441 gctgaatact ttgaaaaagt gttagaattg tcacctgatg atctggaaat tcaaattgat
     4501 tatgcacaat gtctagtgca acttggtatt gctaaaaaag cagaacattt attttatgac
     4561 aatattattt ataataggca tctagaagat agcttttatg aattgagtca gctcaacatt
     4621 gaagttaacg aaccaaacaa ggcattcttg tttggtatta attatgttat tgttagcgac
     4681 gaccaagatt atagagatga attagatcaa atgtttgatg tgaaatatca aagtgaagaa
     4741 caaattgaac ttgaagctca attgtttgta gttcaaatac tattccaata tcttttttct
     4801 caaggtcgat taaaagatgc aaagaattat gtcttacatc aaccacaaga agttcaagat
     4861 catcgtgtgg tacgtaattt attggcaatg tgttatttat atctcggcga atatgatacg
     4921 gctaaagcat tgtacgaagc actattacaa gaggatagta cagatatata tgcattatgc
     4981 cattatactt tgctacttta taacactaag gaaaatgaac aatatcaaaa atatttaaaa
     5041 atattaaata aagttgtacc tatgaatgac gatgaaagtt ttaaattagg tattgtttta
     5101 agttatttaa agcagtatcg tgcatcacaa caattgttgt accctttata taaaaaaggg
     5161 aaatttttat caattcaaat gtacaatgct ttagcatata attattatta tttaggtgaa
     5221 gaagacgaaa gtcattacta ctgggataaa ttgaagcaaa tttctaaagt ggaaattgga
     5281 catgctcctt gggtaattga aaatagcaaa gaagtttttg accaacatat tttgccatta
     5341 cttcaaagtg atgacagtca ttatcgttta tatggtattt ttttattgga tcaattaaat
     5401 ggcaaagaaa ttgtgatgac ggaaagtatt tggcaggttc tggaaaatct aaataattat
     5461 gaaaaattgt atttaacgta tttagttcaa ggtttaacgc tcaataaatt agacttcatt
     5521 catcgcggct tgttaacgct ttaccataat gaattatttg taagtgaaaa tgatttaatg
     5581 gttgcatgga ttaatcaagg tgaactcata attgctgaaa aagtagattt aactgatgtt
     5641 gagccatata tcggtgcgtt tatttatttg tattttaaaa atcaacctcg aaacgttaca
     5701 aagaagcaaa ttacaacatg gttaggcata acacaatata aactgaacaa aatgattgaa
     5761 tttctcttga gcatatagat ttatgaaaag ttagatttat tatataatgc gcttaatgat
     5821 taataatgag gaggcgttaa taaaatgact gaaatagatt ttgatatagc aattatcggt
     5881 gcaggtccag ctggtatgac tgctgcagta tacgcatcac gtgctaattt aaaaacagtt
     5941 atgattgaaa gaggtattcc aggcggtcaa atggctaata cagaagaagt agagaacttc
     6001 cctggtttcg aaatgattac aggtccagat ttatctacaa aaatgtttga acacgctaaa
     6061 aagtttggtg cagtttatca atatggagat attaaatctg tagaagataa aggcgaatat
     6121 aaagtgatta actttggtaa caaagaatta acagcgaaag cggtcattat tgctacaggt
     6181 gcagaataca agaaaattgg tgttccgggt gaacaagaac ttggtggacg cggtgtaagt
     6241 tattgtgcag tatgtgatgg tgcattcttt aaaaataaac gcctattcgt tatcggtggt
     6301 ggtgactcag cagtagaaga gggaacattc ttaactaaat ttgctgacaa agtaacaatc
     6361 gttcaccgtc gtgatgagtt acgtgcacag cgtattttac aagatagagc attcaaaaat
     6421 gataaaatcg actttatttg gagtcatact ttgaaatcaa ttaatgaaaa agacggcaaa
     6481 gtgggttctg tgacattaac gtctacaaaa gatggttcag aagaaacaca cgaggctgat
     6541 ggtgtattca tctatattgg tatgaaacca ttaacagcgc catttaaaga cttaggtatt
     6601 acaaatgatg ttggttatat tgtaacaaaa gatgatatga caacatcagt accaggtatt
     6661 tttgcagcag gagatgttcg cgacaaaggt ttacgccaaa ttgtcactgc tactggcgat
     6721 ggtagtattg cagcgcaaag tgcagcggaa tatattgaac atttaaacga tcaagcttaa
     6781 ttcgaagtcg aattaagatc ttgagctgta aattatttgg atatttattt taatagtgtc
     6841 atcacattgt taaaataatg tcttactttt aaattaaagc aaattatata gaaaactaga
     6901 acttagtacg tatcatttgt gcgtttcaat gagttctagt ttttttatat gttatattaa
     6961 acttataact tt
//
LOCUS       DAHHRR010000122         6959 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.122, whole genome shotgun sequence.
ACCESSION   DAHHRR010000122 DAHHRR010000000
VERSION     DAHHRR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.122"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            61..1365
                     /gene="eno"
                     /locus_tag="NBI42_002226"
     CDS             61..1365
                     /gene="eno"
                     /locus_tag="NBI42_002226"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCD1778620.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEYGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            1704..2162
                     /locus_tag="NBI42_002227"
     CDS             1704..2162
                     /locus_tag="NBI42_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778621.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            2229..2462
                     /gene="secG"
                     /locus_tag="NBI42_002228"
     CDS             2229..2462
                     /gene="secG"
                     /locus_tag="NBI42_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCD1778622.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            2591..3331
                     /locus_tag="NBI42_002229"
     CDS             2591..3331
                     /locus_tag="NBI42_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCD1778623.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPEEILQSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMHHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDKMIDPQSANYIYDHVDSDDKDIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            3365..5737
                     /gene="rnr"
                     /locus_tag="NBI42_002230"
     CDS             3365..5737
                     /gene="rnr"
                     /locus_tag="NBI42_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCD1778624.1"
                     /translation="MNLKQSIEEIINKPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSSKGHSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEATPDNIEDSEIKGRHDLRNELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDDSGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPTIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGHKKKGKQRKGKNERKNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            5759..6223
                     /gene="smpB"
                     /locus_tag="NBI42_002231"
     CDS             5759..6223
                     /gene="smpB"
                     /locus_tag="NBI42_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCD1778625.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            6314..6675
                     /gene="ssrA"
                     /locus_tag="NBI42_002232"
     tmRNA           6314..6675
                     /gene="ssrA"
                     /locus_tag="NBI42_002232"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2425 a          983 c         1406 g         2145 t
ORIGIN      
        1 ttaaacgaaa gaataaagct ataataatta tagaataact atttaaagga gattataaac
       61 atgccaatta ttacagatgt ttacgctcgc gaagtcttag actctcgtgg taacccaact
      121 gttgaagtag aagtattaac tgaaagtggc gcatttggtc gtgcattagt accatcaggt
      181 gcttcaactg gtgaacacga agctgttgaa ttacgtgatg gagacaaatc acgttattta
      241 ggtaaaggtg ttactaaagc agtggaaaac gttaatgaaa tcatcgcacc agaaattatt
      301 gaaggtgaat tttcagtatt agatcaagta tctattgata aaatgatgat cgcattagac
      361 ggtactccaa acaaaggtaa attaggtgca aatgctattt taggtgtatc tatcgcagta
      421 gcacgtgcag cagctgactt attaggtcaa ccactttaca aatatttagg tggatttaat
      481 ggtaagcagt taccagtacc aatgatgaac atcgtaaatg gtggttctca ctcagatgct
      541 ccaattgcat tccaagaatt catgatttta cctgtaggtg ctacaacgtt caaagaatca
      601 ttacgttggg gtactgaaat tttccacaac ttaaaatcaa ttttaagcaa acgtggttta
      661 gaaactgcag taggtgacga aggtggtttc gctcctaaat ttgaaggtac tgaagatgct
      721 gttgaaacaa ttatccaagc aatcgaagca gctggttaca aaccaggtga agaagtattc
      781 ttaggatttg actgtgcatc atcagaattc tatgaaaatg gtgtatatga ctacagtaag
      841 ttcgaaggcg aatacggtgc aaaacgtaca gctgcagaac aagttgacta cttagaacaa
      901 ttagtagaca aatatcctat cattacaatt gaagacggta tggacgaaaa cgactgggat
      961 ggttggaaac aacttacaga acgtatcggt gaccgtgtac aattagtagg tgacgattta
     1021 ttcgtaacaa acactgaaat tttagctaaa ggtattgaaa acggaattgg taactctatc
     1081 ttaattaaag ttaaccaaat cggtacatta actgaaacat tcgatgcaat cgaaatggct
     1141 caaaaagctg gttacacagc agtagtttct caccgttcag gtgaaacaga agatacaaca
     1201 attgctgata tcgctgttgc tacaaacgct ggtcaaatta aaactggttc attatcacgt
     1261 actgaccgta ttgctaaata caatcaatta ttacgtatcg aagatgaatt atttgaaact
     1321 gctaaatatg acggtatcaa atcattctat aacttagata aataatcatt ttaaacatta
     1381 agtactgcca taattttagt tgaggattat tatgtcggta taaattgaat aaagtttttg
     1441 agttcacgct taaaaagttc acgcttgtat ttataacctg ccacagagtt gagactgtgg
     1501 taggtttttt attttgaagt attattcata acagactaat aatcatgagg taactaataa
     1561 catatattta acttgtattc ttaaactggt ataataaatt tatgttgaaa tgaatattgt
     1621 atgacagggt attcactttt attaagaggt aaaattaaat aaaggtatta tagaacgtat
     1681 ttaaatatat gaggagtaaa taaatggctg atagaacgaa taaagaaatt aaaacaggac
     1741 gctttattgc aactgcatca atcgtattct caatattgtt gattattcat tactttgttt
     1801 cgttggataa tgcgactgcc aaagcattac tcaatttaac gaatcaaaac acttcagata
     1861 aagcgataga ttacatttta aacagcttta gatttactgg tattatgtat attttggctt
     1921 atctagcagg cttcatcact ttttggaatc gacatactta tgtgtggtgg tttatgtttg
     1981 cagtttatgt atcaaatagt ttgtttacgt tgattaattt atcaatcaca attcaagcaa
     2041 taaaagctgc acacggtgcg tacttaacat tgccaatttt aattgttatt ataggttcgg
     2101 ttgcattagc gatttatatg cttgttgttt ctatcaaacg taaaagtaca tttaatcgct
     2161 agaaaattga ttttaacaat aaaaatatga tatactactt gtcgtatata aggaacggag
     2221 gacaatttat gcatacattt ttaatcgtat tattaatcat tgattgtatt gcattaataa
     2281 ctgttgtact actccaagaa ggtaaaagca gtggactttc aggtgccatc agtggtggtg
     2341 ctgagcagtt attcggtaaa caaaaacaac gtggcgtcga tttattctta aatagattaa
     2401 caattatttt atcaatatta ttttttgtac ttatgatttg cataagttat cttggtatgt
     2461 aaggtccggc gatgtaaatg tcgggctttt ttatttataa ttagaataat aaaggactca
     2521 tgttcaatta ttagaaacat attgtttaat taagtacgtt tgttaattgt gaaaaaggag
     2581 cgttagattt atgcaaataa agttaccaaa gcctttcttt tttgaggaag gtaaacgtgc
     2641 cgtgttatta ctacatggtt ttacaggcaa ttcgtctgat gttcgtcaat taggtcgatt
     2701 tttacaaaag aaaggttata catcgtatgc accgcaatat gaaggtcatg cagcaccacc
     2761 agaagagata cttcaatcaa gtccttttgt ttggtttaaa gatgccttag atggctatga
     2821 ctaccttgtt gaacaaggtt atgatgaaat cgttgttgct ggcttgtcat taggtggcga
     2881 ttttgcatta aaattaagct taaatcgaga tgtaaagggt attgtaacga tgtgtgctcc
     2941 tatgggtggc aaaactgaag gtgccattta tgaaggcttt ttagagtatg cacgcaattt
     3001 taaaaagtat gagggtaaag atcaagaaac gatcgataac gaaatgcatc atttcaaacc
     3061 aactgaaact ttaaaagaat taagtgaagc attagatacg attaaagagc aagttgatga
     3121 agtgttggat cctattttag tgattcaagc tgaaaacgac aaaatgattg atccacaatc
     3181 cgcaaattat atatatgacc atgtagattc tgatgacaaa gatatcaagt ggtacagtga
     3241 atctggacat gttattacga ttgataaaga gaaagaacaa gtatttgaag atatttatca
     3301 atttttagag tcattagatt ggtcagaata aaaagagatt ttaaaattag aaaggagggg
     3361 cataatgaat ttaaagcaat ctatagaaga aattattaat aaacctgaat atgaaccaat
     3421 gtcggtatca gattttcaag atgcattagg tttaagcagt gccgactcat ttagagattt
     3481 aattaaggtg ctcgtagaat tagaacaatc aggattaatt gaacgtacaa aaacagatag
     3541 ataccaaaaa aagcatagtt caaaaggtca ttcaaaattg ataaagggaa cgctaagtca
     3601 aaataaaaaa ggctttgcat tcttaagacc tgaagatgag gatatggaag atatatttat
     3661 tcccccaaca aaaattaatc gtgccttaga tggagatact gtaattgtag aaatacatca
     3721 atctaaaggt gagcataaag gtaaaattga aggggaagtc aaatcaattg aaaagcattc
     3781 ggtgactcaa gttgttggta cgtatagtga agctagacat ttcggctttg tgattccaga
     3841 tgataaacgt attatgcaag atattttcat tcctaaaggt caaagtttag gcgcagtcga
     3901 tggtcataag gtacttgttc aaattactaa atatgctgat ggttcagata atccagaagg
     3961 acatatttct gctattttag gacataaaaa tgatcctggc gtagatattt tatctattat
     4021 ctatcaacat ggcatagaaa ttgaatttcc tgatgaagtg ttacaagaag ctgaagcaac
     4081 accagacaat atagaagata gtgaaattaa aggacgtcat gatttacgta atgaattgac
     4141 catcactatt gatggcgcgg atgctaaaga cttagatgac gcaattagtg ttaaaaagtt
     4201 agcgaacggt aatacgcaat taactgtaag tattgctgat gtcagctatt atgtaacaga
     4261 aggttctgca ttggataaag aggcttatga tagagctaca agtgtatatc ttgttgaccg
     4321 tgtaattcca atgattccac atcgattaag taatggtatt tgttcattga atcctaatgt
     4381 tgatcgttta actttaagct gtcgcatgga aatcgatgat agtggtcgcg ttgtaaaaca
     4441 tgaaatcttt gatagtgtga ttcattctga ttatcgaatg acgtatgatg cggtaaatca
     4501 gattattact gaaaaggatc ctacgattcg agagcagtat aatgaaatta cgcctatgct
     4561 agatttagca caagatttat ctaatcgttt gattcaaatg agaaaacgcc gtggtgaaat
     4621 tgattttgat attagcgaag cgaaagtgct agttaacgaa gatggtatac ctacagatgt
     4681 tcaattgaga caacgtggtg aaggcgaacg tcttattgag tcatttatgt taattgcaaa
     4741 tgaaacagtt gctgaacatt ttagtaagtt agatgtacca tttatttacc gagtgcatga
     4801 gcaacctaaa tcagatcgct taagacaatt ctttgatttt attacaaact ttggcatcat
     4861 gattaagggt actggcgaag atattcatcc aacaacactt caaaaggttc aagaagaagt
     4921 agaaggtcga cctgaacaaa tggtcatttc aacaatgatg ttacgttcaa tgcaacaagc
     4981 gcattatgat gatgtgaact tgggacattt tggcttatca gctgaatatt atacgcattt
     5041 tacatcacca ataagacgtt atcctgattt aacagttcat cgtttaatcc gtaagtattt
     5101 aattgagaaa tcaatggata acaaagaagt gaagcgttgg gaagacaaat tgcctgagtt
     5161 agctgaacat acttctaaac gtgaacgtcg tgctattgag gcagaacgtg acactgatga
     5221 attgaaaaaa gcagaatata tgattcaaca tattggtgat gaatttgaag gtattgtcag
     5281 ctcagtagct aacttcggta tgttcattga attgccaaat acgatagaag gtatggttca
     5341 tattgcgaat atgacggatg attattaccg ttttgaagag cgtcaaatgg cattaattgg
     5401 tgagcgtcaa gctaaagtat ttagaattgg ggacacagtt aaggttaaag tgacgcatgt
     5461 tgatgtagat gaacgattaa ttgattttca aattgtaggt atgcctttac cgaaaaatga
     5521 tcgatcacag cgcccagcgc gaggtaagac aatacaagct aaaacgcgtg gtaaatcatt
     5581 agataaatcg aaatctgatg ataaaggtca taagaaaaaa ggtaagcaac gtaaaggtaa
     5641 aaatgaacgt aaaaatgaca aatcaggaaa cagtaaacat aagccatttt ataaagataa
     5701 aagtgtgaaa aagaaagcac gtcgtaagaa aaaataagca gcaatgaggt gagtatgaat
     5761 ggctaagaag aaatcaccag gtacattagc ggaaaatcgt aaagctagac atgattataa
     5821 tattgaagac acgattgaag cgggaattgt attgcaaggc acagaaataa aatcaattcg
     5881 ccgaggtagt gctaacctta aagatagtta tgcgcaagtt aaaaacggtg aaatgtattt
     5941 gaataatatg catatagcac catacgaaga agggaatcgt tttaatcacg atcctcttcg
     6001 ttctcgaaaa ttattattgc ataagcgtga aatcattaaa ttgggtgacc aaacacgtga
     6061 gattggttat tcgattgtgc cgttaaaact ttatttgaag catggacatt gtaaagtatt
     6121 acttggtgtt gcacgaggta agaaaaaata tgataaacgt caagctttga aagaaaaagc
     6181 agtcaaacga gatgttgcgc gcgatatgaa agcccgttat taagcgattt agttgcttaa
     6241 tcgggctata tttgatatag ttatatatgc ttttgtaaat tacaaaagta tgatttgttt
     6301 gatttattat ttcgggggcg tttctggatt cgacaggggt cccccgagct cattaagcgt
     6361 gtcggagggt tgtcttcgtc atcaacacac acagtttata ataactggca aaacaaacaa
     6421 taatttcgca gtagctgcct aatcgcactc tgcatcgcct aacagcattt cctatgtgct
     6481 gttaacgcga ttcaacctta ataggatatg ctaaacactg ccgtttgaag tctgtttaga
     6541 agaaacttaa tcaagctagc atcatgttgg ttgtttatca cttttcatga tgcgaaacct
     6601 ttcgataaac tacacacgta gaaagatgtg tatcaggacc tctggacgcg ggttcaaatc
     6661 ccgccgtctc catatttgta gcctacaacc cttgtggatg tgggcttttt attatgtgtt
     6721 tttcagggga taatgcattg tagaaatagt tgtgagtatt gaaacagcag tgtttgtata
     6781 ggtgtttatt tgatggagga aagagtaata agtgattatg aattagtttt tgagatataa
     6841 gaggaaaggg atgtgtgtca aataagtgtc aaaaaagttg agctttgagt tttagaatgt
     6901 aaattttatt tgcaaaatta attaagctaa agaagtatca taaataagaa atttaaata
//
LOCUS       DAHHRR010000123         6774 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.123, whole genome shotgun sequence.
ACCESSION   DAHHRR010000123 DAHHRR010000000
VERSION     DAHHRR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.123"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            58..984
                     /locus_tag="NBI42_002233"
     CDS             58..984
                     /locus_tag="NBI42_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1778626.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            984..2054
                     /locus_tag="NBI42_002234"
     CDS             984..2054
                     /locus_tag="NBI42_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1778627.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRKLDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMISLIIIVIFAFIGPVINKHDYAEQNVQHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMVLSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            2070..3152
                     /locus_tag="NBI42_002235"
     CDS             2070..3152
                     /locus_tag="NBI42_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1778628.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            3142..4083
                     /locus_tag="NBI42_002236"
     CDS             3142..4083
                     /locus_tag="NBI42_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1778629.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFEIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQREKGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPADDIYQYPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EASNHLRQLHEIRPQHFVFSTEEEAARLRKNLLVTQD"
     gene            4102..5757
                     /locus_tag="NBI42_002237"
     CDS             4102..5757
                     /locus_tag="NBI42_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1778630.1"
                     /translation="MTRKLKTLILILVATITLSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRNDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALD
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNELTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            5926..>6774
                     /locus_tag="NBI42_002238"
     CDS             5926..>6774
                     /locus_tag="NBI42_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1778631.1"
                     /translation="MIKYFLKRIFYMAISLVIITTITFFLMKLMPGSPFNDAKLSPDQ
                     KYILNEKYGLNDPIPIQYLNYMKNVFTGDFGYSFQYHNQPVWDIIKPRLIPSLEMGIT
                     AMIIGVLIGLILGVASAAKQNTWIDYCATLISVLAVSVPSFVLAVLLQFVFAVKLRWF
                     PVAGWEGISTVILPSFALSAIVIATVTRYIRAELLEVLKSDYILLARAKGVPNKRVLY
                     KHALRNALIPLVTVLVPMMASILTGTLTIENIFGVPGLGDQFVRSIATNDFPVIMATT
                     ILFSSFF"
BASE COUNT         2404 a          956 c         1289 g         2125 t
ORIGIN      
        1 aaaattttga agggaatgga aatttagaaa tcggaatttg ttagaggagg ggattagatg
       61 gggaaatata tttttaaacg gtttatttat atgcttattt ctttatttat tattattaca
      121 attacatttt tcttaatgaa attaatgcca ggttcgccat ttaacgatgc taaattaaat
      181 gctgaacaaa aagaaatttt aaatgaaaaa tatggattaa atgatcctgt agctacgcag
      241 tatttacatt atttaaaaaa tgttgttaca ggcgattttg gtaattcatt ccagtatcat
      301 aatcaacctg tgtgggattt gattaaaccg agactactac cttcttttga aatgggtctt
      361 acagcaatgt tcatcggtgt gatactggga cttattttag gtgttgcagc agctactaaa
      421 caaaattctt gggttgacta tacaactaca gttatttcag ttattgcagt atctgtacca
      481 tcttttgtac ttgctgtact tttacaatat gtatttgcag ttaaattaag atggttccca
      541 gtagctggat gggaaggttt ttcgaccgcg gtattaccgt cacttgcatt atctgcagct
      601 gttttagcaa ctgtcgccag atacataaga gcagagatga tagaggtatt aagttcagac
      661 tacattttat tagcgagagc taaaggtaat tcgacaatgc gtgtactttt tggacatgca
      721 cttagaaatg ctttaattcc aattattaca attatcgttc ctatgttagc aagtatttta
      781 acaggcactt taacaattga aaatattttt ggagttcctg gattagggga tcaattcgta
      841 cgttcaatta caacaaatga tttctcagta atcatggcaa tcacactatt atttagcaca
      901 ttgtttatcg tttctatttt tattgtagat attttgtacg gtgtgataga tccacgaatt
      961 cgtgttcaag gaggtaaaaa ataatggctg aaaataaaaa caatttgtcg attaacgacg
     1021 atcattctaa tgcagctatg acgcatacct ctgacgctat tgcatcatct gattttatta
     1081 ttagaaaatt agatttgaat caggaacctg aaatgcaacg agaaagcaaa aacttttggc
     1141 aagatgcttg ggctcagtta aaacgaaata agttagctgt tgtcggtatg ataagtttaa
     1201 ttatcattgt aatatttgct tttatcggtc cagttataaa taaacatgat tatgctgaac
     1261 aaaatgtaca acatagaaat cttccggcaa aaatacctgt attagacaaa gttccatttt
     1321 taccttttga tggtaaagat gcagatggca aggatgctta taaagcagca aatgctaaag
     1381 aaaattattg gtttggtact gatcagttgg gtcgagattt atggacaaga acatggaaag
     1441 gtgctcaaat ttcattgttt atcggtgttg ttgctgcgat gttagatatt tttattggcg
     1501 ttgtatatgg tgcgatttct ggattcttcg gtggacgtgt cgatacgatt atgcaacgta
     1561 tacttgaagt catagcatct attccgaatt taattgttgt aattttattt gtattaattt
     1621 ttgaaccatc catttggaca attatattgg ctatgtctat cacaggctgg ttaggcatga
     1681 gcagagttgt acgtggagaa tttttaaaat tgaaaaatca agagtttgtc atggcttcga
     1741 aaacattggg ggcttcaaaa ttcaaattga tatttaagca tattttacct aatacgttag
     1801 gtgctatcgt ggttacatca atgtttacag tacctagtgc tattttcttc gaagcatttt
     1861 taagtttcat tggtataggt gtacccgcac ctcaaacatc gttagggtca ttagtaaatg
     1921 atgggcgcgc aatgttatta atttatccac atgaattatt cataccagca atggttttaa
     1981 gcttattaat tttattcttt tacttattta gtgatggatt acgtgatgca tttgatccga
     2041 aaatgcgtaa ataaaagggg gcatagcata tgactgaaag aatattagaa gtaaatgatt
     2101 tgcatgtttc ctttgatatt acagcagggg aagtgcaggc agtgagaggt gtagattttt
     2161 atttaaacaa aggggaaaca ttggcaattg ttggtgaatc aggttcaggt aaatctgtga
     2221 caacaaaagc aattacaaaa ttattccaag gggacacagg aagaattaaa aagggagaaa
     2281 ttttattttt aggggaagat ttagcaaaaa aacctgaaaa tgagttaatt aaattacgtg
     2341 gcaaagatat ttcaatgatc tttcaagatc caatgacatc tttaaaccca acgatgcaaa
     2401 ttggtaaaca agtcatggaa ccattaatta agcacaaaaa ttatagtaaa gcacaagcta
     2461 aaaagcgcgc attggaaata ctaaatcttg taggtttacc aaatgcagaa aaaagattta
     2521 aagcatatcc acatcaattt tcaggtggac aaaggcaaag aattgttatt gcaaccgcat
     2581 tagcttgtga acctaaagtg ctcattgctg atgaaccaac gactgcatta gacgtaacga
     2641 tgcaggcaca aattttagat ttaatgaaag aactacaaca aaaaatcgat acagcaatta
     2701 tttttataac gcatgattta ggggttgttg cgaatattgc tgatagagtg gcagttatgt
     2761 atggtgggca aatggttgaa acaggtgatg ttaacgaaat attttatgat ccaaagcatc
     2821 catatacatg gggattatta tcgtcaatgc ctgatttatc aacaactaat gacacaccat
     2881 tactagcgat tcctggagcg ccacctgatt tattacaccc acctaaaggt gatgcatttg
     2941 cgagacgtag tcaatatgca ttagatattg attttaaagt agaaccaccg tggtttaaag
     3001 tttcaccgac acattttgtg aaatcttggt tattagacgc acgtgcacca aaagttgaac
     3061 tacccgagct ggtaaaacaa cgtatgaaac cgatgcctaa taattatgaa aaaccactca
     3121 aggtagaaag ggtgtcgttc aatgaaaaat gatgaagtgc tattatctat taaaaattta
     3181 aagcaatatt ttaacgcagg aaagaaaaac gaagtgagag cgattgaaaa tatttcgttt
     3241 gaaatataca aaggggaaac attaggttta gtaggagaat cggggtgtgg taaatctaca
     3301 actggtaaat caattatcaa acttaatgat attacaagtg gagaaatttt gtatgaaggt
     3361 attgatatac aaaagattcg taaacgtaaa gatttgctta aatttaataa aaagatacag
     3421 atgatttttc aagacccata tgcgtcttta aatcctaggt taaaagtaat ggatatagta
     3481 gctgaaggta ttgatatcca tcatttagca actgataaac gtgaccgtaa aaaacgtgtc
     3541 tatgatttac ttgaaactgt tggattaagt aaagaacatg ccaatcgtta tcctcatgaa
     3601 ttttcaggtg gacaacgcca acgtattgga attgcccgtg cattagccgt tgaaccagaa
     3661 tttattatag cagatgaacc tatttcagca ttggatgttt caattcaagc tcaagtagtc
     3721 aatttgttat taaaattaca gcgtgaaaaa ggtattacat tcctatttat tgcacatgat
     3781 ttatcaatgg tgaagtatat ctctgatcga attgcagtca tgcattttgg gaaaattgta
     3841 gaaatcggac cagcagacga tatttatcaa tatccattac atgattatac taagtcttta
     3901 ttgtcggcaa ttcctcaacc ggatcctgaa tcggaacgta gtcgtaaacg atttagttat
     3961 attgatgatg aagcaagtaa tcatttaaga caattacatg aaattagacc acagcatttt
     4021 gtctttagta ctgaagaaga agcggcacga ctacgaaaaa atttgttggt gacacaagat
     4081 taagggaaag ggggaaatga aatgacaaga aagttaaaaa cgctgatttt aatattggtt
     4141 gccacaatta cattaagtgg ttgtgctaat gatgatggta tttattcaga taaaggtcaa
     4201 gtattcagaa aaattttgtc atcagactta acatcccttg atacatcatt aattacggat
     4261 gaaatatctt ctgaagtgac agcgcaaaca ttcgaaggtt tatatacatt aggaaaaggt
     4321 gacaaaccgg tgttaggtgt tgcgaaagct tttcctgaaa agagtaaaga tggtaaaact
     4381 ttaaaggtta aattaagaaa cgatgctaaa tggagcaatg gtgacaaagt gactgcacaa
     4441 gactttgttt atgcttggag aaaaacagtt gaccctaaaa caggttctga atttgcatac
     4501 attatggggg acattaaaaa tgcgagtgat attagtactg gtaagaaacc tgtagagcaa
     4561 ttaggtatca aagcattaga tgatgaaaca ttacaaattg aattagaaaa gccggtacca
     4621 tatattaatc aattattagc actaaataca tttgcacctc aaaatgaaaa agttgccaaa
     4681 aaatatggta aaaattacgg tacggcagct gatagagcgg tatacaatgg tccatttaaa
     4741 gttgatgatt ggaaacaaga agataaaacc ttactatcta aaaatcagta ttattgggat
     4801 aaaaagaatg taaaattaga taaagtgaat tataaagtta ttaaagactt acaagcaggt
     4861 gcatcattgt atgatactga atcagtagat gacgcagtta ttacagcaga tcaagtaaat
     4921 aaatataaag ataataaagg attaaacttt gtgttaacga ctgggacatt ttttgtgaaa
     4981 atgaatgaaa aacaatatcc tgattttaaa aacaaaaatt taagactggc tatcgcacaa
     5041 gcaatagata aaaaaggata cgttgattca gtgaaaaaca atggttcaat tccttccgat
     5101 acattaacag ctaaaggcat tgcgaaagcg ccgaatggca aagattatgc aagtaccatg
     5161 aattcacctc taaaatataa tcctaaagaa gcaagagcac actgggacaa agctaaaaaa
     5221 gagttaggta aaaatgaatt gacattttca atgaacacag aagatacacc agatgcaaaa
     5281 atatctgctg aatatatcaa atcgcaagtt gagaaaaatt taccaggagt tactttgaaa
     5341 attaagcaat tgccgtttaa acaaagagta tcactagaac tgagtaacaa ttttgaagca
     5401 tcacttagtg gttggtctgc agattaccct gatcctatgg cttacttaga aacaatgacc
     5461 acaggtagcg cacaaaataa tacagactgg ggtaataaag aatacgatca attacttaaa
     5521 gtagcaagaa ccaaattggc acttcaaccg aacgaacgat atgaaaactt gaaaaaagca
     5581 gaagaaatgt tcctaggaga tgcaccagta gcaccaattt atcaaaaagg tgttgcacat
     5641 ttaacaaatc ctcaagtaaa aggattgatt taccataaat ttggtccaaa taactcactt
     5701 aaacatgtat atattgataa atcgatagat aaagaaacag gtaagaagaa aaaataatat
     5761 gctttgtaaa ttaggctgga gacatatctc cagtcttttt gtattgcaga aaaactttgg
     5821 aaataaaaaa tgggaaacaa taaaaaaatg ttgaaaataa ataaaatagc tatataatag
     5881 tgttaatatt ctgaaaattg agttagatgg agtgtataga ttgcgatgat taaatatttt
     5941 ttgaagagaa ttttttatat ggcaatttca ttagtaatta ttacaacaat aacttttttt
     6001 ctaatgaaat taatgcctgg ttcacctttt aatgatgcta aattaagtcc ggatcaaaaa
     6061 tatattttaa atgagaaata tggtttaaat gaccctatac caatacaata tttaaattac
     6121 atgaaaaatg tatttacagg ggattttggt tattcttttc agtatcataa tcaaccagtg
     6181 tgggacatta tcaaaccaag gttaatccct tctttggaaa tgggcattac agcaatgata
     6241 ataggagtgt tgattggatt aattttagga gttgcttctg ctgcaaaaca aaatacttgg
     6301 atagattatt gtgcaacact tatatcagta ttggcagtgt ctgttccttc tttcgtactt
     6361 gcagttttat tacaatttgt atttgcagtt aaattaagat ggtttcctgt agctggttgg
     6421 gaaggcattt caacagttat actgccttct tttgcgctat ctgctattgt tattgcgaca
     6481 gtcactagat atattcgtgc cgaactttta gaggtgttaa aaagtgatta tatactgttg
     6541 gctagggcaa aaggtgttcc taataaaagg gttttatata aacacgcttt aagaaatgct
     6601 cttatcccac ttgtaacagt gctagtacca atgatggcta gtatattaac tggtacatta
     6661 actattgaaa atatttttgg agtcccaggg ttaggagatc aatttgtacg ttctattgca
     6721 acaaatgatt tcccagtaat aatggctaca actattttat ttagtagttt tttt
//
LOCUS       DAHHRR010000124         6685 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.124, whole genome shotgun sequence.
ACCESSION   DAHHRR010000124 DAHHRR010000000
VERSION     DAHHRR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.124"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            163..348
                     /locus_tag="NBI42_002239"
     CDS             163..348
                     /locus_tag="NBI42_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778632.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            439..1335
                     /locus_tag="NBI42_002240"
     CDS             439..1335
                     /locus_tag="NBI42_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCD1778633.1"
                     /translation="MLKKAKLILIATLLLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVHCDDH
                     GIVFNASLPLYKDAIYQKGSMRSNDNGNDMSMMVGTVLSGFEYRAQKEKYDNLYKFFK
                     ENEKKYQYTGFTNEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIHKNDKEFKE
                     GMQRARKEVNYAANTDAVATLFSTKKNFTKDNTIDDVIELSNKLKDKPNMPQKSQVTI
                     QLGKPSINTKKPFYDDINPIEG"
     gene            1347..1752
                     /locus_tag="NBI42_002241"
                     /pseudo
     CDS             1347..1752
                     /locus_tag="NBI42_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829176.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1806..2732
                     /locus_tag="NBI42_002242"
     CDS             1806..2732
                     /locus_tag="NBI42_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCD1778634.1"
                     /translation="MFKKTKLILIATILLSGCSAMESESKKDTNTEIKSVPEEMEASK
                     YVGQGFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CDDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEKKYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIHKNDK
                     EFKEGMQRARKELNYTANSNTVATLFSTNDKKNRKEKINNVIDLSEKIESTKDMPFKN
                     TITTQLGNKLIGTKKARFDDKKVVSFGAFEDE"
     regulatory      3143..3277
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3339..3884
                     /locus_tag="NBI42_002243"
     CDS             3339..3884
                     /locus_tag="NBI42_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD1778635.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIIVALVKNLLNYLFNMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IIISIVFILLYRRLANFLKRI"
     gene            complement(3990..4238)
                     /locus_tag="NBI42_002244"
     CDS             complement(3990..4238)
                     /locus_tag="NBI42_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCD1778636.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            4346..5299
                     /locus_tag="NBI42_002245"
     CDS             4346..5299
                     /locus_tag="NBI42_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1778637.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTM
                     QQDFRFVPISQNNKIQETVKIVYNYIKENKLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTRLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVEQEDQQQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            5289..6668
                     /locus_tag="NBI42_002246"
     CDS             5289..6668
                     /locus_tag="NBI42_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCD1778638.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQDVLTTLHSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIVILNKKKVIRSIGFD
                     EKLQNLFLR"
BASE COUNT         2569 a          943 c         1133 g         2040 t
ORIGIN      
        1 tggttatttt ttcagggcaa aaaaagggcg gattatttaa ataaggacaa acacttgtgg
       61 aaaatttaaa aggttaaaaa taataaagaa cttggtataa caagggtttt atacatttgc
      121 gtacaacgac gaaatgtcaa tttaccatca cattatgacg atatgtttat tttaaacaca
      181 caagctcatg cgcgtcttga tcaaatggca caacagtttg aagttgtttg taatggcttg
      241 aacgaaaatg aaggacaagc tattcaaaca atggatcaat ctgcgtcgtt aattcgttca
      301 aacttaattc aagttaaaga acaattagaa aaactagcag tatactaatt aatttattaa
      361 aagctacttg ttttcattga aaataagtag ttttttcaaa cataaaagtt ttacaaacac
      421 ataaatgggt gatgagatat gcttaaaaag gcgaaattaa tcttaatagc aacgctactg
      481 ttatcaggat gttcaactac caacaacgaa tccaacaatg aaacaaaatc agtaccagaa
      541 gaaatggaag cttccaaata tgtaggacaa ggcttccaac cacctgcaga aaaagatgcg
      601 attgaatttg cgaagaagca tcgtaaagaa tttgaaaaag taggtgaaca attctttaaa
      661 gataactttg gactaaaagt taaagctaca aatgttgtag gtaaagatga tggtgtagaa
      721 gtttatgtgc attgcgatga tcatggcatt gtatttaatg caagtttgcc cttgtacaaa
      781 gatgccatct atcaaaaagg atcaatgcgt agtaatgaca atggtaatga tatgagtatg
      841 atggtgggta cagtgcttag tggctttgaa tatcgagcgc aaaaagaaaa gtatgacaat
      901 ttatataaat ttttcaaaga gaatgaaaag aaatatcaat atacaggatt tacaaacgag
      961 gcaattaaca agacacaaaa cgtcgggtat caaaatgaat acttttatat tacatattta
     1021 tcaagaaact taaaagaata tcgtaaatat tacgaaccat tgatacataa aaatgataaa
     1081 gagtttaaag aaggtatgca acgagctaga aaagaagtga attacgctgc taatacagat
     1141 gctgttgcta cacttttttc aactaagaaa aactttacta aagacaatac aatagatgat
     1201 gtgattgaat tgagtaataa attgaaagat aaacccaata tgcctcaaaa atctcaagtt
     1261 actattcaat taggaaaacc aagtataaat actaaaaagc cgttttatga cgatataaat
     1321 ccaattgaag gctgagtaaa tataaagtga ataaaataaa tgatagagac atagcagaat
     1381 tgagcagtta ttgagtttat ctagatataa atgaaaaaag attttaaaga aaatggaaaa
     1441 cggttcaaac aaatagatta ttataatgat aaaaataacg ataatatgaa aggtgcagtt
     1501 gatataaaaa cctatgagct acttaatgat cagggagatc caactggtca acagacaata
     1561 atatatcaag gaacatctaa agaggtaatt aatccagata atccattaaa atcatagggt
     1621 attggagatg attggcttca aaatgctaaa ttaatgaata attaaattaa aatcagagat
     1681 tatttaacac aagtagatgt acttggaggt atatatatag gtgaactaaa agatgcaaat
     1741 aaattaagtg agtataacta tatgcaaaat gaaattttta aaagcgcata aatgggtgat
     1801 gagatttgtt taaaaagaca aaattaatct taatagcaac aatactgcta tcaggatgtt
     1861 ccgcgatgga aagtgaatca aaaaaagaca cgaatacaga aataaaatca gtaccagaag
     1921 aaatggaagc ttcaaaatat gtaggacaag gcttccaacc gcctgcagaa aaagatgcga
     1981 ttgaatttgc gaagaagcat cgtaaagaat ttgaaaaagt aggtgaacaa ttctttaaag
     2041 acaactttgg gctgaaggta aaggcaacca atgttgtagg taaagatgat ggtgtagaag
     2101 tttatgtgca ttgcgatgat catggcattg tatttaatgc aagtctacct ttgtacaaag
     2161 acgccatcca tcaaaaagga tcaatgcgta gtaatgacaa tggtgatgat atgagtatga
     2221 tggtgggtac agtgcttagt ggctttgaat atcgagcgca aaaagaaaag tatgataact
     2281 tatataaatt tttcaaagaa aatgaaaaga aatatcaata tacaggtttt actaaagaag
     2341 caattaacaa gacgcaaaac gtcgggtatc aaaatgaata tttttatatc acatatttat
     2401 caagaaattt aaaagaatat cgtaaatatt atgaaccatt gattcataaa aatgataaag
     2461 aatttaaaga gggtatgcaa cgagctagaa aagagctaaa ttatacagct aatagtaata
     2521 cagtagcaac gttgtttagt acgaatgata aaaaaaatag aaaagaaaag ataaataatg
     2581 taatagattt atccgagaaa attgaaagta caaaagatat gccattcaaa aatactataa
     2641 ctactcaatt aggaaataaa cttattggca caaaaaaagc tcgttttgat gataagaaag
     2701 tagtgtcgtt tggagcattt gaagatgaat aaaattaatg atgaaaattt aaccgagaat
     2761 agtgtatatt gagtagatca agaattaaaa gataattcta ctattaaagt gaatggaaat
     2821 aagtagaaga ttttaagtat aacttctggc gatttaatta ataatataaa tgtaaatact
     2881 gatataaagc tttttgacct actagattct tataatttgc tttccatttt atgacgatct
     2941 tgacctaatt gtgtggtagg ttcaaagaag ccatcccaac aacagttaaa accacaaacc
     3001 aatcaaacct atccaaacaa tgctcgcaaa tcaccaatga aacactctcc aattacgtaa
     3061 ctatgatcta atttaagcat agttattgag gttttgtgat atatagtata aaattaatga
     3121 gaattaaatt taataatgtt aaattcatct tcggggtcgg gtgtaattcc caaccggcag
     3181 taaattaagc ctgcgacctg ctagtatgtt tcatattagt ggctgatcta gtgagattct
     3241 agagccgaca gtatagtctg gatgggagaa gatggaggtt ttttgttgtg caataatcct
     3301 ccttttctta cgagatgaat ggaaggagaa aattgaatat gcaacaaaat aaacgtctta
     3361 tcacaataag tatgttgagc gcgattgcgt ttgtgttaac ttttatcaag tttcctatac
     3421 catttttgcc accatactta actttagatt ttagtgatgt accgtcattg ctagctacat
     3481 ttacgtttgg accagtggct ggaatcatag ttgcactggt taaaaactta ttgaattact
     3541 tatttaatat gggtgatcca gttggtccat ttgcaaactt tttagcggga gcaagtttct
     3601 tattaacagc gtacgctatc tataaaaata aacgttcaac aaaatctttg attactggat
     3661 taatcatcgc aacaatcgtt atgactatcg tgttgagtat cttaaactat ttcgttctat
     3721 tacctttata tggcatgata ttcaacttag ctgatattgc gaataatctt aaagtaatta
     3781 ttgtttcagg aattattcca ttcaatatta taaaaggtat cattatttca attgtgttta
     3841 tcttattata tagaagactg gcaaatttct tgaaaagaat ttaatcaaat taaagcaaaa
     3901 taatataaac ataataataa aaagcaggtg actatcaata aacgatagct tgcctgcttt
     3961 ttctatataa catttgtcta aaaaatcaat tattcaaatt ttaaagcgtc cccatcaaat
     4021 gattcgtctg caattttaat agaatctgta gggcatccat caattgcatc ttccatatct
     4081 tcatataatt cctcaggtac ttctgcagta ccttggttat cgtcaaggat tacgaaagca
     4141 ataccttcgt cgtcgtaatc atatatatct ggcgctgctg caccgcatgc accacatgca
     4201 atacaagtat ccatatcaac gattgtatat tttgccaatg tcttcgcctc ctttgataaa
     4261 aatgctaaaa tagtaatgtg actaaaattt tagacagcat catttttatt ttcaaattat
     4321 ccgttttaca gagtgagggt taaatttgca acacattata aaaacagcat tacaacaaac
     4381 atttaactat aaaacaaata aaagtattta caatatctta gttggtaaga aatctcacca
     4441 aacctttttt gacgcttgta gtcaacaaca gttgtcatta tatcacagtt taccactatt
     4501 aaaatatccg tcttttgagc tatttttaga aaaaatcaat gaatttaatg ctgaaatgga
     4561 aatcatgttg catcctagat atacatttga aagtatgggg caaacatttc aagcaattca
     4621 actattagtg caaaccatgt ctaatactat gcagcaagat tttcgtttcg taccaatctc
     4681 tcaaaataat aagatacaag aaactgtaaa gattgtctat aactacatta aagaaaacaa
     4741 actacaaatt gattttgaaa atgaactaca taacttgttt aaagcaataa ctttaaaagg
     4801 gccatgttac ttacattatt atttgcaagg ctatgatgaa ccaatgtata cgagacagca
     4861 agttagttta atagaaaaac tatctcaaca gcaattgttt gaatacgaaa tgaataattt
     4921 agtgacaatg atgtttgaat tagaaagtgg agaatatact attttatcaa aaataataat
     4981 gaaacctaca ttattaaatc aaacctatat tacttataca agattgcttg aacaattcac
     5041 gatggaagat atagcggctc aacaacaagt taaaatcaat actatcgaag atcatgtact
     5101 tgaaatctta atcaaaggtt atatgtctaa ttacgatgat tatgttgaac aagaagatca
     5161 acagcaattt ttgaattttt atcaacagca tcgtggcgaa cgattaaaat tttacaaaga
     5221 acaatttgac acgttatcat attttcaatt aaaagtatta atcgttggat ttgaaagagg
     5281 tgatctgaat gttgcatgat attttacgaa acaaatttgg tttcgagagt tttaaaccgg
     5341 gacaacagga aattatagaa agtataatgt ctcaacaaca cactctaggt attcttccaa
     5401 ctggaagcgg aaagagtttg tgttatcaaa tacctacgta tttatcaggt aaaccgacat
     5461 taattatctc accgttaata tctttaatgg atgaccaagt tatgcagttg aaaataaatg
     5521 gagaaaaacg tgtaacatgt attcactctg gtatggatga aattgagaaa aagcataata
     5581 ttaaatgttt acgacatagc cgcttcatct ttctaagtcc agaatttctc ctgcaaccgt
     5641 caaattttaa attaatatct atgatagact ttggcatgat tgttctagat gaagcacatt
     5701 gcctatctga atggggatat gatttcagac cacattatgc tctaatagga aaagtaacaa
     5761 agcattttaa agaagcggtt gtcttagcat tgacagcaac tgcaccaccg catttacaag
     5821 atgatttgac ggaaatgtta gcgattcaat tcaatgttat taaaactaca atgaatcgcc
     5881 caaacataag ctttaagcat cttaattttc atgatgatga agataaaatt gagtggttgc
     5941 tgccgtttct acaacagtcg ggaccaacga ttatttatgt ctcatcgaaa aagatgtgtc
     6001 tgaatttagc gcaacttatt tatgattcag gttttcttac aggcatatat catggtgata
     6061 tgaattatca agagcgacac acagttcaac aacaattttt aaataatgat attccgatta
     6121 tagtcgcaac gagtgctttt ggtatgggaa ttaataaaaa agatattcgc acaatcattc
     6181 actttcatct ttcaacaagt ccttctaact acattcaaga aattggacgt gcgggtcgcg
     6241 atggtgaact aagtcaggca attagtttat ttcaaccgga cgataaatat attttagaaa
     6301 cgttattatt tgcagatatg ataacagaag aagatgtaca aaatttcgaa ataggagaat
     6361 ttttagcacc cgataaacaa gacgttttga caacgttgca ttcattctat agtatcggcg
     6421 ccttgaaaca gatatttaag caatcattta aacgaaagca attaggattc tttcgcatga
     6481 ttggctattg caaattggat caatgtagac ggaagtattt attagaattt ttcggtgaat
     6541 atccaccggc acaagatcga tgttgtgaca atgattctaa tataactgat atcgtaattt
     6601 taaataagaa gaaggtaatt agaagtattg gatttgatga aaagttgcaa aatttatttc
     6661 tcagatagta ttactttact aaaag
//
LOCUS       DAHHRR010000125         6541 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.125, whole genome shotgun sequence.
ACCESSION   DAHHRR010000125 DAHHRR010000000
VERSION     DAHHRR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.125"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            394..1011
                     /locus_tag="NBI42_002247"
     CDS             394..1011
                     /locus_tag="NBI42_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HCD1778639.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
     gene            1027..2034
                     /locus_tag="NBI42_002248"
     CDS             1027..2034
                     /locus_tag="NBI42_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCD1778640.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(2621..3418)
                     /locus_tag="NBI42_002249"
     CDS             complement(2621..3418)
                     /locus_tag="NBI42_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD1778641.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
     gene            3779..4423
                     /locus_tag="NBI42_002250"
     CDS             3779..4423
                     /locus_tag="NBI42_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCD1778642.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
     gene            5118..>6541
                     /locus_tag="NBI42_002251"
     CDS             5118..>6541
                     /locus_tag="NBI42_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1778643.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADVRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKCLMLKPEPLVGKMVQEIKE
                     IILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQINTYTEMKLVFLEGIFKTLLNKN
                     SQVVFFIMPSILKSKNTMSEEEKFAIIKTIIESDLKIAFNINDIETTYYVEEAFMSVF
                     RQISPNELSNHNNYEVHFVFDLSLMEIRTIYRMILKLHNIMLNVKLGLNITCLFEKPS
                     VFKSLVSQIKRLKFDSLIIDNANL"
BASE COUNT         2324 a          832 c         1047 g         2338 t
ORIGIN      
        1 ttatgtaata aatattgtat cgttgcaaat ttcccaacta tatccattta caatttttag
       61 atttttatag ataaaaaatt gcctatacaa caactttttc aaaacaaatg cctactacta
      121 tttaaatttt atgtaaaagt taagtttcgg aattgttaaa aatttgtaaa ttgaattgtg
      181 ggattgtaaa ttttgtgcta ttttcatata agggcaaata cagtaaattg tttactgtga
      241 ggcatttttg aaattaatat cagtacacta aaattatact gacgattgat aataacaaat
      301 ttgtatcatt agtttgtaaa ttcacttgtc ttaatttgaa acaaattata actgaatgtg
      361 attggtgaca atcgcttaaa tggaggattt taaatgttta gtaagaaaaa agataagttt
      421 atggttcaat tagaagagat ggttttcaat ctggatcgtg ctgctattga attcggtaaa
      481 atggatttca atacacattt agatttaaaa gcatactcag acaacattaa aacttatgag
      541 tcacatggtg acgaattagt acatcaagta attactgatt taaatcaaac atttatcaca
      601 ccaattgaac gtgaagatat tttatcatta tgtgatgcaa ttgatgatgt tttagatgca
      661 attgaagaaa cggcagctat gtttgaaatg tattcaatcg aatacacaga tgaatatatg
      721 gctgagtttg ttgataacat tcaaaaagca gttgcagaaa tgaaacttgc tgtcggctta
      781 ttagtcgata aaaaattatc acatatgcgt attcattcaa ttaatattaa agaatttgaa
      841 acaaactgtg atggtatttt aagacagtca attaaacata ttttcaatag cgaaacagat
      901 ccaatcacat taattaaaat aaaagatatt tatgaaagca tggaagaaat tgctgataaa
      961 tgtcaaatcg tagcaaataa ttttgaaact attattatga aaaatagcta aggggagtat
     1021 atatttatgt catatataat catcgtcact atagctgtag ttattttctc gctgatattt
     1081 gactttatca atggattcca tgatacagcc aatgcagtag ctactgctgt atctactaga
     1141 gcgttaacgc ctaaaacggc aattttaatg gcagcagtga tgaactttat aggtgcttta
     1201 acatttacgg gcgttgcagg caccattact aaagacattg tcgatccatt taaattggaa
     1261 aatggattag ttgttgtgtt agctgcaata cttgcggcta ttatttggaa tttagctact
     1321 tggttttacg gaattccaag ttcgtcttca catgcactta taggttcaat tgcgggtgca
     1381 gcaatcgcat ctgaaggctc atttggagtg ttacattacc aaggtttcac aaaaattatt
     1441 attgtattaa tcgtttcgcc gattatcgca ttttgtgttg gtttcttgat gtattcaatt
     1501 tttaaagtta tctttaaaaa tgcaaattta acaagagcga atcgtaactt tagatttttc
     1561 caaattttca cagcagcgtt acaatcattc tctcacggta cgaatgatgc gcaaaaatca
     1621 atgggtatta ttacgttggc attgattgtt gctaatgtac agaatgatgg cagcgttgaa
     1681 ccacagttat gggtaaaatt tgcctgtgcg acagcaatgg ggcttggtac tgcaattggt
     1741 ggctggaaaa ttatcaaaac tgtaggtggt aatattatga aaatacgtcc tgcaaatggt
     1801 gcagcggccg atttatcatc tgcattaaca atttttgttg catcatcgct acatttccca
     1861 ttatcaacaa ctcacgttgt gtcatcatca atcttaggtg ttggtgcttc taaccgagct
     1921 aaaggtgtaa aatggagcac tgcgcaacga atgatcatta catgggtgat tacattacct
     1981 atttcagcat tgttagcagg tttactattc tatatactta acttattttt ctaattgaaa
     2041 ataaaactaa actgaacttc agtatcacaa acatttggtg gtattgaagt tcatttttta
     2101 tgtaagtaga taacatattt tatagaattt acgtagaatg accaagacaa agcattttaa
     2161 ttagggggct aagttataaa tttattccat ggaacataaa cagttaggtc attaaaatat
     2221 aagtaagcgt aaaataatga tttaatagtt tatattcttc aatgaaggaa taattaaata
     2281 gttatcaact tgtttaatta acctaaattg tattggactt tatttaatat aaatttgtat
     2341 atgtagaaga aaaagcgtaa aatactggta taacaaatta aatcattata tagtcaactt
     2401 agttgcatta ttttgatttt taatatatta tgcatttaat ttaatcaaat aggtaggttg
     2461 ttaattttta cctaactacc attcatacaa aatataaata tgtatgatag ggaagggtga
     2521 gataattttt tgtaccaaaa tgaaaatttt tttcaaaaat aaaaaactag caatctcaca
     2581 tgatgtgaat tgctagtata tatcagtaca atttatttaa ttaatggatg aatgcatagc
     2641 tagaaacttc tgaagctgga attgtacggt agttcatatt gtatggacca tatgtgtaat
     2701 tcatttcaga aatcaagata ctaccatcac cattgacacg ttcaacataa gcaacatgac
     2761 catatgggcc aggtgtgctt tgcataattg aaccaactga tggtgtgttg tttacttggt
     2821 aaccatcatt agctgcgtta ccagcccaat acttagcgtc tgaccaatat gtactaattg
     2881 gactaccagc ttgagcacga cggtcaaata cgtaccatgt acattgacca gcagtgtata
     2941 aattttggtg attaaaagat gatgcattgc cattgctacc tgttgtagct gttggtgttg
     3001 taccacctga tccaccatta ggaatttgta atgtttggtt aggcataatt aaataaccac
     3061 gtaagttatt ggctgccatt aattgatcaa ctgaaacacc atatctgcta gcaatgatat
     3121 ttaatgattc accagcttgt acagtatgag atgatgctga accagcttgt ggagaagtgt
     3181 ttgacgtatt ttgtgcatca cttccaccta ctgagataac ttgaccaggg aataccaagt
     3241 tgttatctaa ttggttattt tgtttaatac tctctactga agtgttgtat ttttgagcaa
     3301 tactccataa tgattcacca gattgtactg tatgttgtgt agaagcttgt gcatcatgat
     3361 gcgttaaaaa tgcagctgca ccagatgttg ctgttattgc aaatgctaat tttttcaaag
     3421 ggactcctcc ttaaaattaa gatttactac ttttaaaaat tattaagaca atttatatag
     3481 taaattaagt tttcgtttta tattacgctt tccatattat caaaaataag tagctttgtg
     3541 tggtttgtta ttatcttgta atattattaa cattttagga tatcggttca tatttttata
     3601 aatgctgatt tgatagtgta taaaagcgtt ttagtgattt taactaatta taaaaaatta
     3661 tattttgtaa cattgtaaaa tgtaaaatta ctatacttgt taaaggttat ttcttttgat
     3721 gaagcaaaat ttactgtaaa atttgacgca taaaacgaca gtagaaggag gaattatttt
     3781 gtcgcaaaat acaaatcatt catattatca tcaaaaccag catgctcaat caataagtaa
     3841 agtgtggctt tattttatgt attattggat tatatttggc ataggatgct atttaggtca
     3901 gtttttacca ttaagttggc gacaaccctt gtcatttgga ttactgatta ttattttagc
     3961 aacacttgtt tttgaaagag cgagacggtt cggtttaatt atttcacata tttatgctgt
     4021 agtgattggc ttattgtcat acgcaacctt taccacgtat ttacaaaatt taggaccaga
     4081 tattttctat aaaaatatcg cattagcaat ttttgcattt atagcatttg gtattattgg
     4141 ttatttcttc gttggagacg catcgagtat aggcaagtat ttattcgtta cattaataac
     4201 attaattatt gcgagtctaa ttggtatttt tcttcaaaat cctatttttt atactattat
     4261 taccgtcgtt agtctgttgt tatttctact ttatactttg tatgatttta atcgtttaaa
     4321 aagaggtgac tattcaccaa gagaaatggg atttaatcta tttattaatt tgttgaatat
     4381 tattaaggat atactttatc ttgctaatat gttcagaaga taaacagtta aattaataaa
     4441 gaaatttgta taaagtttgg aataatcaat aatgaatatc aaccttaaat tggagcgatt
     4501 gtcataaatt agaaatttag atagaggttt tactgataaa gcaataaaaa tatattacct
     4561 taattgtaaa gatattatta atcattgaaa tgattaactg ctatagttct aaagtaaaca
     4621 aatcttagaa gataaacttt aaacaggtct acttgccttt ctaattacac tttattagaa
     4681 aagtaaagta tagctacgta ttaattttaa aatcgaaata ttaataggaa taagtataga
     4741 taatataaat aatttaatgt tagtttgagc tcatctcaaa agtagaatgt gatttaaata
     4801 agtgtttatt tttaaattga aaatgaatat aaaactaaca aaagataata gtaatacgtt
     4861 gtagatgtgg tcatttaaaa ttattttgaa aatactaaaa agtagtgtgt tttcaattgg
     4921 atagtttaat taaactgact acatctattt ttgtaatatt taaatgattg atatataagt
     4981 ttaaaaatta accattatat agttttattt atttttgatt tggtattcgt attgtgttaa
     5041 atttacgttt aattaaataa gttaaatatt atagggaatg ataaaagtgg tacttaaata
     5101 tagaaagagg taaagttatg gcaaaatcat gcttgcatat acttactaat aatgaatatg
     5161 cgacaacgcg ttgccaagat ggcatagtct tattttggcc aattgacggg gaaatcgaat
     5221 tacaaaaatt tcgtaaaagt aaaataattg aagatgatat atatattatt aatcatctgg
     5281 atgtatttag tattaagaat aataaaaaaa cgatcatgtt gtatttgagt agcgattggt
     5341 ttgcggaatt aggctttact ttctttaatt accactatac agcaaagtta attaaatcat
     5401 cctataattt gaaatgtcta ctattaaaat tgacttatcg ataccttgat aatcaacctc
     5461 tcaatgacgc tgatgttaga aaattacagg atattattaa aattattgca aaagaagcaa
     5521 gtatggataa aaagattgcg caaaatcaat atcgatatgc ctattatggt gatttgcgtg
     5581 acgagctcga atatatttat caaaatgtaa atcaacgatt gacattaaaa agtgtcgctg
     5641 ataaattatt tgtttcaaag tcaaatttgt catcacaatt ccacttactt atgggcatgg
     5701 gttttaaaaa atatattgat actttgaaaa ttggtaaatc gattgaaatt ctacttacta
     5761 ctgatagtac tattagcaac ataagtgaac atttaggttt tagtagtagc tcaacttact
     5821 ctaaaatgtt taaaagttat atggatataa caccgaatga atatcgtaat ttatcaaaat
     5881 ataataaatg tttaatgcta aagccagaac cactagtagg caaaatggtg caagaaataa
     5941 aagaaatcat attgaattat attgaacatt ataaaaacca cctaactgat gttatacata
     6001 ttgatgagga caaatttgaa acacctaaat tgtttcaaac ggttattcaa ataaatactt
     6061 atacagaaat gaaattagtt ttcttagaag gaatctttaa aaccttattg aataagaaca
     6121 gtcaagttgt ctttttcatc atgccatcga ttttaaaaag taaaaatacc atgtccgaag
     6181 aagaaaaatt cgcaattatt aaaacaataa ttgaaagtga tttaaagata gcatttaata
     6241 taaatgatat tgaaacaact tattatgttg aagaagcttt tatgagtgtt tttagacaaa
     6301 tatctccaaa cgaattaagt aatcataata attacgaagt gcattttgtt tttgatttat
     6361 cattgatgga aattagaaca atttatcgaa tgatattaaa attacataac atcatgttga
     6421 atgtgaaatt aggattgaac attacctgtt tatttgaaaa accttcagtt tttaaatcac
     6481 tagtatcaca aataaagcga cttaaattcg attcgttaat aatagataat gcaaatttaa
     6541 g
//
LOCUS       DAHHRR010000126         6425 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.126, whole genome shotgun sequence.
ACCESSION   DAHHRR010000126 DAHHRR010000000
VERSION     DAHHRR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.126"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            199..513
                     /locus_tag="NBI42_002252"
     CDS             199..513
                     /locus_tag="NBI42_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778644.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQVLDAIVGYKSKDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            complement(847..2244)
                     /gene="sufB"
                     /locus_tag="NBI42_002253"
     CDS             complement(847..2244)
                     /gene="sufB"
                     /locus_tag="NBI42_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCD1778645.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            complement(2395..2859)
                     /locus_tag="NBI42_002254"
     CDS             complement(2395..2859)
                     /locus_tag="NBI42_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCD1778646.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEGGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            complement(2849..4099)
                     /locus_tag="NBI42_002255"
     CDS             complement(2849..4099)
                     /locus_tag="NBI42_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD1778647.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNAILKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDCRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            complement(4205..5512)
                     /gene="sufD"
                     /locus_tag="NBI42_002256"
     CDS             complement(4205..5512)
                     /gene="sufD"
                     /locus_tag="NBI42_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCD1778648.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNAIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            complement(5610..6371)
                     /gene="sufC"
                     /locus_tag="NBI42_002257"
     CDS             complement(5610..6371)
                     /gene="sufC"
                     /locus_tag="NBI42_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCD1778649.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
BASE COUNT         1962 a         1241 c          904 g         2318 t
ORIGIN      
        1 atgctatagt tcgagctgtt tgttattaat aaatttacat aatgaaaaat ttcaacaaat
       61 tcagtttatg acatagttat aatcatttga aaccattata atgataacaa ccagtttctc
      121 ataaatttat tctaattatc ttttaaataa ctttagacaa gtacttgttt atattcaaat
      181 atagaaagaa ggattaacat gtattttgtt ttagcaatat ttacaatcat tagtgccagt
      241 gtaagtttag gttattcaat tcaagcatgt gcatctagcc ataatataaa tgcatattat
      301 gcacttagtc gaagcttacc tttattttta ttagctattt tttctttagt cattcatagt
      361 gctatatttt tgataactat atccattgca atgattttag ttcaagtttt agatgcgatt
      421 gttggttata aaagtaaaga tgtctttaaa acatacggtc cattagcaac atctgtagtg
      481 aacttaatat tattaatagt tttcttattt taactcactt atacaacgaa tcttaatcga
      541 actaatatcg aattatcttt aaagaatatt tgatttagtt ttcaattaat ttaataaaga
      601 ccagcactct aatgccacaa tcatgttgta tttgtgttgt cgctttgtcc accatcaatg
      661 attatttttt acactaatca aaaaaatcgg actgatataa atacgcacaa agcttatcta
      721 tcaatccgat ttagttataa aaccaaaaaa agccacatta atgtggcttt ttgttatatt
      781 cagtatcaaa atggtatcaa tagccatttc cggaagtcaa gaatggctta acaacgcggt
      841 ttaaagctat ccaatactac cttccatttc gaacttgatt aaacggttca tttcgaccgc
      901 gtattccatt ggaagttctt ttgtaaatgg ttcgatgaat cccataacaa tcatttctgt
      961 cgcttcttct tcagaaatac cacgactcat tagatagaat aattgttctt cagaaacttt
     1021 tgaaaccttc gcttcatgtt ctaatgatat ttgatcgttg aatacttcgt tatatggaat
     1081 tgtatctgat gttgattcgt tatctaagat taatgtatca cactcaatat ttgaacgagc
     1141 gccttttgct ttacgtccaa aatgtacaat accacgataa ataactttac caccattttt
     1201 agaaatagat ttagaaacaa ttgttgaaga tgtatttggc gctttatgaa tcattttagc
     1261 accggcatct tgaacttgtc ctttaccagc aaatgcaata gataatgtac taccttttgc
     1321 accttcacct aaaagaacac agtttggata tttcatcgtt aacttagaac ctaagttacc
     1381 atctacccat tccatatttc cgttttcata aacaaaagta cgttttgtaa ctaaattgta
     1441 cacattgttc gcccagtttt gaatcgtagt ataacgaacg tgcgcatctt tatgcacaat
     1501 gatttccaca acagcagagt gtaaagaact agttgtataa actggtgcag tacaaccttc
     1561 tacgtaatgt acagaagcac cttcatcagc aatgattaat gtacgttcaa attgacccat
     1621 gttctcagag ttaatacgga aataagcttg tagtggcgta tctagtttga tatttttagg
     1681 tacataaatg aacgaaccac ctgaccatac tgctgagttt aacgccgcaa atttgttatc
     1741 tgctgcaggt actacagaag caaagtattt tttgaataat tcttcatttt cttgtaaagc
     1801 actatctgta tctttaaaga taataccttt ttcttcaagt tctttttcca tattatggta
     1861 aacaacttca gattcatatt gagcagaaac gccagctaaa tatttttgtt cagcttcagg
     1921 aattcctaat ttatcgaaag ttcttttaat ttcttctggc acttcatccc atgaacgttc
     1981 agcttgttct gaaggcttta catagtaagt aatgtcatcg aaattcaatt ctgataagtc
     2041 gccaccccat tgaggcattg gcattttata aaacaatttt aatgatttaa gacggaaatc
     2101 taacatccat tccggctcat ttttcatgtt agaaatttct ctaacgatat tctcagttaa
     2161 accacgttct gatctgaaaa tggacacatc atcgtcgtgg aatccatatt tataatcccc
     2221 aacatcaggt gcttttttag ccatttcaat cactccttaa aaatatattt acaacggcgt
     2281 gcgtaagttt ttaattcatg atgtaaacca tattataaca atgacatgac atcttataaa
     2341 aatttttata ctcttatatg tctaatatca aaatcatcta tgattaacag cattctattc
     2401 ttcttcagtc gtaccttctg ctttaccttc tttagcaaca gtaccttttt ccaatgcttt
     2461 ccaagctaat gtggcacatt taatacgagc tgggaattga gatacacctt gcaatgcttc
     2521 aatatctccc atttcttctg taatcacata gtcttcacca agcatcattt tcgtaaattc
     2581 ttggctcatt tgcattgctt ctccaagtga atgaccttta acagcttgtg tcatcatcga
     2641 tgcacttgcc attgaaatcg aacaaccttc accttcaaac ttagcatctt ttataatgcc
     2701 gccttctata tcaaatgtta gtcgtatacg gtcaccgcat gtcgggttat tcatatctac
     2761 tgtcatagac ccgttatcta atacaccttt atttctagga tttttataat gatccataat
     2821 gacagatcta tataattgat ctagattatt aaaattcata agagaaaaac tccttcgttt
     2881 gtttcaaggc atttattaac tgatcaacgt cttctttcgt gttgtatata taaaaactcg
     2941 ctctagctgt tgaagacaca tttaaccatt tcattaacgg ttgtgcacaa tgatgcccag
     3001 ctctaaccgc tacaccttct gtatctacgg ctgtagcaac atcgtgtgga tgtacatctt
     3061 gtaaattaaa cgttattaca cctgcacgac aatcctttgg cgggccataa atttcaattc
     3121 cttcaattgc agacatttgc tcataagcat atatcgttaa ttcttgttca tatttatgaa
     3181 ttgcatcaaa acctatgcgt tctaaatagc gaatagcttc tgcaagccca attgcttgag
     3241 caattaatgg agtacccgcc tcaaatttag taggtaaatc agcccatgtt gcatcatact
     3301 tacttacaaa atcaatcatg tcgccaccaa actcaatcgg ttccattttt tgtagtaact
     3361 cacgtttacc aaataatacg ccaatacctg ttggtccaag cattttatgt ccactaaaac
     3421 tataaaaatc agcattcatt tcttgcatat caagtttcat atgtggtgct gcttgcgccc
     3481 catcaacact gataattgcg ccatgttgat gagctatttc tgcaatggtt ttaacatcat
     3541 taattgtacc gagtacatta gaaatatgtg caatagcaac gatctttgtt ttatcattaa
     3601 tcgtttgctt aatatcttcg atgtttaatt ccccgtcagc tgtcattggt ataaatttca
     3661 atatcgcatt tttacgcttt gctaactgtt gccaaggaac aatattggca tgatgttcca
     3721 tttcagtgac aacaatttca tcgccctctt caacatttgc atcaccatag ctatgtgcta
     3781 caaggttaat cgatgcagtt gttccgcgtg tgaaaatgat ttcttcaaaa tacttcgcat
     3841 taataaaacg acgaacggtt tcacgggcat tttcataacc atcagttgcc aatgatccta
     3901 atgtatgaac accacgatga acgtttgaat tataacgctt gtagtaatct tctaaaacat
     3961 ttaacacttg cacaggcgtt tgacttgtcg ctgttgaatc aagatatgct aaacgtttgc
     4021 cattgacttt ttgatctaat atcggaaaat ccttgattac ttcattaacg tcaaatgagt
     4081 gttcggccac ttcattcacg acctttctta aataaaaatc ctaatcattt aaatactgac
     4141 gttgtattag tcttatacca atatcgacag tctatatcta ttacaaactt ttattttcaa
     4201 aatattattt agaaactttg cgttcaatta cttctctcaa ttgacgttta acgtcttcga
     4261 taggtaattc acgtactact ggatctaaga aaccatgtat aacaagacgt tccgcttctc
     4321 tttgagaaat accacgactc attaaatagt aaagttgatc tggatcaaca cgacctactg
     4381 atgcagcatg accagcttgt acatcatctt catcaattaa taaaattgga ttcgcgtcac
     4441 cacgagcatg ttcagataac attaatacac gtgattcctg attagcaatt gatttagttc
     4501 caccatgctt aatgtagccg ataccattaa atacagatga tgcatgttct ttcataacac
     4561 catgtttaag gatataacca tctgtttctt taccatattg tacgatttta gatgttagat
     4621 taattttttg ttcgcctgta cctacaacta ctgatttaag tgaacttgtt gaacgatcac
     4681 caaataaatt tgttgtatta tcaataattt ggctaccctc attcattaaa cctagtgccc
     4741 aattaattga ggcatccgct tcagtaatac cacgtcgaat gatatgacct gtaaagcctt
     4801 tatccatata gtctactgag ccatatgtga tatttgaatt tgcaccagca atcacttcag
     4861 aaataatatt taattgattt ccttcaccag atgcatttga taagtaattt tcaacatatg
     4921 tgacttcggc gctttcttca gtaacgataa tgacatggtt ataaaagctt gcattttcgt
     4981 cgtcgtgtaa cacaacgtat tgtactggat gttctacaac tacattttta ggaacataaa
     5041 caaatacgcc accattaact aatgccgtgt gtagcgctgt gatacgatgt tcatctactg
     5101 ttactgcatc tttcataaag tacttttgta ctaaatcact atggttcata agagcgtctg
     5161 ctaaaccttc aacgataacg ccatctttcg atgcattatc atcaacttgt gtgtacgcaa
     5221 tcgcattatt atgttgaatt actaagtttt tagaatgatc tacgtcaata atttctctta
     5281 ctgactcagg taattgtgat aaagattgat aaacatcacc ttttacatcg tgttgtttaa
     5341 aagaatcaaa atcccatttt cttaattttg ttttatcagg ttttggcatt tctaaagttt
     5401 ctgttaattt caaagctttt ttacgtaatt ctgtcatcca agaaggttca ttgtgggctt
     5461 tagaataatc aacaagttgt tcttcagaaa tgttcaaaat atcagttgtc ataacttttt
     5521 cctcctactt gatgaaattt actatttcgt ataaatgttc attttcatca tatatagatg
     5581 aacatctcat ggatactgta ttaataagat tattcagctg aaccgaactc ttctttaacc
     5641 cattcatatc cttcttcttc aagacgtttt gctaattctg gaccaccaga tttaacgacc
     5701 ttaccagcat acattacatg tactttatca ggagtaatgt aatttaataa tcgttgatag
     5761 tgtgtgatca ttaatgcacc aaagttttcc ccacgcattt ggttaatacc tttagataca
     5821 acttttaatg catcgatgtc taaccctgaa tcgatttcat ctaagattgc aaacttaggt
     5881 tctaacatca ttaattgtaa gatttcgtta cgtttcttct ctccacctga gaaaccttca
     5941 tttaaataac gttgtgccat gtctttatct atgtctagaa aatccatatt tttatctaat
     6001 ttcttaataa attgcattaa gttgatttct tgtccttctt cacgtttcgc attgatagct
     6061 gaacgcatga aatcagcatt tgtaacacct gtaatttctg atggatattg cattgccaag
     6121 aataatcctg cttttgctct ttcatcaact tctaattcta aaatatttac accgtctaaa
     6181 agtacttctc ctttagttac ttcatagctt gggtgtccca taattgcaga tgataaagtt
     6241 gatttacctg tcccgtttgg tcccataatc gcatgtattt catcagtgtt aattgtcaag
     6301 ttaacacctt ttaagatttc tttatcctca atagacacat gtaggtcttt gatttctaat
     6361 gttgatgcca ttttatattc cctccgtaaa atataaagtt ttcttaacta gtttataata
     6421 atttt
//
LOCUS       DAHHRR010000127         6413 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.127, whole genome shotgun sequence.
ACCESSION   DAHHRR010000127 DAHHRR010000000
VERSION     DAHHRR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.127"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..1750)
                     /locus_tag="NBI42_002258"
     CDS             complement(<1..1750)
                     /locus_tag="NBI42_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCD1778650.1"
                     /translation="MSRLLNDFNQSLHKGFIDKYISHKGNYAPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKNEFDLLWQNSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKNKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPTKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEK
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYK"
     gene            complement(1752..2144)
                     /locus_tag="NBI42_002259"
     CDS             complement(1752..2144)
                     /locus_tag="NBI42_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HCD1778651.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEEMSLPLMWEFPGGKIEKNE
                     TEKEALIREIREEMKCDLIVGDKVITTEHEYDFGIVKLTTYKCTLNRELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            2276..4084
                     /locus_tag="NBI42_002260"
     CDS             2276..4084
                     /locus_tag="NBI42_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCD1778652.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNNPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     IIVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLKSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVKYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGLEGKKKI
                     NEIMTKFRSNPPQEIQGMKVKAIEDYLTSEVYQLDKDTMSQINSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
     gene            4368..4634
                     /locus_tag="NBI42_002261"
     CDS             4368..4634
                     /locus_tag="NBI42_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778653.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            4778..5086
                     /locus_tag="NBI42_002262"
     CDS             4778..5086
                     /locus_tag="NBI42_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778654.1"
                     /translation="MTVNIVVSIVFCISMVILGIYVAITKDFTLISYINQTTIADKHK
                     NQIAYIFALCISLSAVFLMSAILCFEYDFIALSFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(5200..>6413)
                     /locus_tag="NBI42_002263"
     CDS             complement(5200..>6413)
                     /locus_tag="NBI42_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly(glycerol-phosphate)
                     alpha-glucosyltransferase"
                     /protein_id="HCD1778655.1"
                     /translation="KNVNNYIGKYKNYKYKFSKEFYNSKDVIKFFTKSFFYDGEEIYE
                     LKSEGYQKGLREVDKLDVEINKMIETSSAFLKNMLNENGKYDYGYFPHFDKNINFYNI
                     LRHSSSTYALIEGLNYLNKPLDCVKKAIDFIIDNYIYEKGDKAFIFDDTNNINEIKLG
                     QNASFIFAVCEYLKVHENGYYLKKAQKVANGILYMIDENKWDTTHVLNYPDLSIKEKF
                     RIIYYDGEAALALMRLFQQDNNEKWLNIVRKLVDQFIFKEYFKYHDHWLGYCTNELVQ
                     IYPEAKYFEFGIKNVSTYLDYINNRETTFPTFLEMLMATYKLIQKAKIEGYGELVESL
                     IDENELLSVINKRANYQRTGYFYPEIAMYFKNPSRVLGSFFIKHHGYRVRIDDIEHYI
                     SGYVQYQKVFN"
BASE COUNT         2161 a         1058 c          828 g         2366 t
ORIGIN      
        1 tcttataatt atctttattt tgcgattgat ccccagaaag cgcaatcgga attaaataat
       61 ttgtcttata attaccaata aaatcaatta cagttacata ctctttattt gaactttttc
      121 ttaatcctct accaagttgc tgaatgaata taatgctaga ctctgttggt ctcaacatta
      181 caacttgatt gacttctgga atatcaatac cttcgttaaa tagatctaca gttataatgt
      241 agttaatctt accttctttt aatttttcta ttacaatttg acgataatta acactatcgt
      301 ctcctgttaa agcaacactt ttaatccctt ttgaacttaa tttatccgct aaatcataag
      361 cttctttttt gctactcaca aaaattaatc cttgtaaaat ttcacctgaa tatccatagt
      421 aatctgtctt ttgaataata taattaactc tttcatcaga agttaaatat ctcagtttag
      481 ttacatcatc ttctttaata ccttgatgta cataatcagt cacaccaaaa taatgaaatg
      541 gacataaaat atcactttct aaggctgctt gtaaccttat ttcatatgca atattataat
      601 caaacagttc aaatatactt aattcgtctg atctttctgg agttgcagtc attcccagca
      661 tgaacttagg tttgaagtaa ttaaatactc gttgataagt agatgcagca gaacgatgtg
      721 cttcatcaaa aacaatataa tcaaattctt tttcatcaaa ttgcttaaaa ttatcatctc
      781 tagataatgt ttgaatggtt gcaaataaat atttggcatc gacatctcta tgtttgcctg
      841 tcaacaatcc aaaatcacta tcattttttg taggtaatac ttttttaaat tcttccttag
      901 ctctatttaa aatcccctca ttatgaacaa taaataaaaa tttattaggg tttacttctc
      961 taacatctaa tgcacataaa atcgttttac ctgtaccagt tgcagatatt attaacgcct
     1021 tatttttggc tttatcccta atagctttta atgaccttaa tgcttctgct tgcattaaat
     1081 tcggtacaat ttccactgat tttttcacct tatcagctag cagcatttga gtttgttcaa
     1141 cttccgctaa tttttctaag gaacggtact caaatgattc tttatatgaa ttaatccatt
     1201 gctcagtcag tggcgtactg ttttgccata acaagtcaaa ttcatttttc acactatcaa
     1261 ctagatcgcc atttttcata gtagacagta aaacattatg ctcataatta acctttaacg
     1321 cattagatgt taaattagag cttcctatta ccatagaact ataatcctta tgttcaaaaa
     1381 tatatccttt ggcatggaat ccagcaatat cagttaatct tacctctaca ttttttaatt
     1441 taagtaattc tccatacatt ttaggactat taaatcctaa gtaattagat gttaatattt
     1501 tccctttaac acccttattg cttaaatcta atagttgagc ctttaagctg gctaacccgc
     1561 tttctgttat aaaagctaca gaaaaataaa acgtttcaca tttttgaagt tcatctataa
     1621 ttgttgaaag aactttttca tttttattat ttactaaaag cttcggtgca taattccctt
     1681 tatgagaaat atatttgtct ataaaccctt tatgtaaaga ttgattgaaa tcatttagta
     1741 atctactcat attatccctc agtcataatt ttattaacgg ctggtatatc cgctggggcc
     1801 caatttaatt tatcaagttc gtttattgac aaccattcaa tactcttatg ttcagttaga
     1861 gttggtaact ctctgtttaa agtacatttg tatgttgtta acttaacaat gccaaaatca
     1921 tattcatgtt ctgtagttat aactttgtct ccaacaatta gatcacattt catttcttct
     1981 ctaatttctc taatcaaagc ctctttttca gtttcattct tttcaatctt accgccagga
     2041 aattcccaca ttaaaggcag actcatttct tcacttctct gtgcacaaag aattttgtta
     2101 tcagaaaaaa taatagctcc tactacattg attacttttt tcataagact cacccttcaa
     2161 tttaaaatca tcttaattgt tattctatca aaaattacaa aaatatatat aaatcaatat
     2221 taaaaattaa tattttacaa tttcctgcat gatatatcac tattacatct actccatgca
     2281 ttttcataca catctattat ataatacttg tgaaaagtat tgtcttgggg ctgtgttttt
     2341 ttacttttgg ggcgtatttc tttataattc attacataaa tgtaagggct ttagttttca
     2401 tgttttatta agtctaactg agattttgaa aggatgttta gcaacaatgg ataaagaatt
     2461 atggatagaa agagctaatg atagtttagt taaacatttt tatgagcagc aatctgacat
     2521 tgagcagcga gaaggttttg aaagtaaatt aacgtttggt actgcgggta tacgcggaaa
     2581 attcggtctt ggtgaaggtc gacttaataa gtttactatt gaaaaattgg cattaggttt
     2641 agcgcgttat ttaaatgccc aaacaaacaa tccaacaata gtcattcatt atgatattag
     2701 acatctttca actgaattcg cccaaattat tgctaatgta ttagcaaacc atcaaataat
     2761 agtttattta cctgatacat ataaaacgac accggaatta tcgttcgcgg tgcgcaatct
     2821 taatactact gctggcatta tgattacagc aagtcataat ccgaaagact ataacggcat
     2881 taaagtatat ggttctgatg gagcgcaact atcgactgat gcatctgagc ttgcaagtcg
     2941 ttatatcgaa gaagttggcg atccattaca gattgatata cctatttcaa aacaaaatac
     3001 atcttatatc aagccatttc cgaaatctgt aactgatgac tatatgaaac acattcaaaa
     3061 tatgattggc tatattccta aatctgattt acaagttgtg ttcactagtt tgcatggtac
     3121 aagtgttccg attgtacctg agttattaaa atctttgaat ttcaatcaat ttaatcttgt
     3181 agaagcacaa tgtaaacctg atccgaattt tagctcagtt caaagtgcta atcctgaaga
     3241 tcatcgcgct tttgatcaag cagtcgaact tgcaaataaa agtcatgcag acttattaat
     3301 atcaactgac cctgatgcgg atcgtcttgg cattgctgaa cgtgatgcac atggtcatat
     3361 cacgtatttt aatggcaacc aaattggtgc gctgttactt aattatcgta ttcaacaaac
     3421 gtctcaatta cgtcatcgct taatgattca atctattgta agtagtgagt taacaaagtc
     3481 attagcacgc tataataatg ttaaatacaa agaggtactg acgggcttta aatttattgc
     3541 tcaggaaata agacaactgg atgatcatca aaatatgatt ttcgcctttg aagagagtta
     3601 tggttttcta tcggagcctt ttgtacgtga taaagatgcc gtacaaattg tgccactcat
     3661 tataaaatac gcttctgagt taaaattata tggcaaaaca ttgaaagatg aattagaaca
     3721 gatttatcaa actgttggta gacatgagga tacattattc tcgcatacgc tagaaggatt
     3781 agaagggaaa aagaaaatta atgaaataat gaccaaattc cgttcaaatc caccgcaaga
     3841 gattcaagga atgaaagtga aagcaattga agattattta actagcgagg tttatcagtt
     3901 ggataaagat acaatgtcgc agattaattc tcctaaatca aatgttattc gtgtcttatt
     3961 tgacgaagga tttatcgctt tacgtccttc tggtactgaa cctaaaatta aattatatgt
     4021 gtcgttaaaa tgtccgaatt ttgatgatgt agctcaaaaa attaatgcga tgatttttag
     4081 ctaattaaaa attttataca tcgaaatatt acatttcatt ttatacttat cagccctttt
     4141 gaaactagaa tccttatgtt ttcgcgtttt gaaggggcta atttgtaata atttttcaaa
     4201 agtatattag aaaattaatt ttcatgtatt tttcatatta agcggtaata atataataat
     4261 taataccgcg acctatttaa ttgttttcgc gatgaaaaag gtttagaatt acaaaaataa
     4321 ctattttaaa tttagttaca aatatatttt agaaaggatg tgaaataatg aaaggctcta
     4381 aacaaatact tttgattatg ggcattatat ctcttattgt tttatttatt tttacactat
     4441 tcatcatggc gcaatatgca aaacattatg aacaaaaatc cgacagttcc aacgcacata
     4501 ctttaaattc atcatcagcc ataattgagc aacatactat gtcgaattta gcgtctctag
     4561 atttatacgc tcctgttcgt aacattactt ctagtcgtga tattcatccc atttatttca
     4621 tgacaaaaga ttaaaacttc ctttttcata accccattca ttcaactaat aaattaaaaa
     4681 atgataaaaa cgtactgttt tttagaaaca tacatatata agaaacgcat ccattactaa
     4741 aagacagtac attatattct atcgggaagg tgcaacaatg accgtcaaca tcgtagtttc
     4801 tatcgtgttc tgtatcagta tggttatatt aggcatttat gtcgcaataa ctaaagattt
     4861 cacactaatt tcttatataa atcaaacgac cattgcagat aaacacaaga accaaattgc
     4921 atatatattt gcgttatgta tcagtttgag tgcagtattt ttaatgtctg caattctatg
     4981 ctttgaatat gattttattg cattgtcatt cttattttta acgattgcat tattacttat
     5041 cgcattattc tatgtgtgct tttataaaat tacaaaatat ccataaatgt ttgtgatagt
     5101 gtgttggtta gatttgatgg tttactgtca ataagcattg tttgcgaaaa aaatacaacc
     5161 ttgtacgaat acaaagttgc attaatatag aaaaaggtat caattaaaca ccttttgata
     5221 ttgtacatat cctgaaatat agtgttcaat gtcatctatt ctaactctat agccatgatg
     5281 tttaataaag aagctcccta atactctaga tggattttta aaatacatcg caatttcagg
     5341 ataaaaatat cctgttcttt gataatttgc acgtttatta ataacagaca acaattcatt
     5401 ttcatcaatt aaagattcta ctaattcacc ataaccttca atcttagctt tttgtattaa
     5461 tttatatgtt gccattaaca tttctagaaa tgttggaaac gtcgtttcac gattatttat
     5521 gtaatctaaa taagtactca cattttttat accaaattca aagtatttag cttccggata
     5581 aatctggaca agttcatttg tgcaatatcc tagccaatga tcatggtatt taaaatattc
     5641 tttaaaaata aactgatcaa ctaatttcct aactatgtta agccattttt cattattatc
     5701 ttgttgaaat aatctcatta aagctaaagc agcttcccca tcataataaa taattctgaa
     5761 tttttctttt atagataaat ctggataatt taaaacatga gttgtatccc atttattttc
     5821 atcaatcata tacaatatac catttgctac tttctgtgct tttttaagat aataaccatt
     5881 ttcgtgaact tttaaatatt cacaaactgc aaatataaaa gaagcatttt gacctaattt
     5941 aatttcattt atattatttg tatcatcaaa tataaaagct ttatctccct tttcataaat
     6001 ataattatct attataaaat ctatagcctt tttcacacaa tcaagcggct tattcaaata
     6061 atttaatcct tcaattaatg cataagttga tgaagaatgg cgtaaaatat tgtaaaaatt
     6121 aatattttta tcgaaatgtg gaaaatagcc ataatcatat ttaccatttt cattcaacat
     6181 attttttaaa aaagcactgc tagtttctat cattttatta atttccacat ctagtttatc
     6241 aacttccctt aatccctttt ggtaaccttc agattttagt tcataaatct cttctccatc
     6301 ataaaaaaaa gatttcgtaa aaaatttaat tacgtcctta gaattataga attctttaga
     6361 aaatttgtat ttataatttt tgtatttacc aatataatta ttcacatttt ttt
//
LOCUS       DAHHRR010000128         6323 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.128, whole genome shotgun sequence.
ACCESSION   DAHHRR010000128 DAHHRR010000000
VERSION     DAHHRR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.128"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            128..961
                     /locus_tag="NBI42_002264"
     CDS             128..961
                     /locus_tag="NBI42_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD1778656.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKVKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            1243..2040
                     /locus_tag="NBI42_002265"
     CDS             1243..2040
                     /locus_tag="NBI42_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1778657.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDKFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            2076..3080
                     /locus_tag="NBI42_002266"
     CDS             2076..3080
                     /locus_tag="NBI42_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1778658.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGLLVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            3077..4093
                     /locus_tag="NBI42_002267"
     CDS             3077..4093
                     /locus_tag="NBI42_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1778659.1"
                     /translation="MISSNNKRRQWIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITAALIIFIFSLNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIILQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            4326..5294
                     /gene="dhaK"
                     /locus_tag="NBI42_002268"
     CDS             4326..5294
                     /gene="dhaK"
                     /locus_tag="NBI42_002268"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCD1778660.1"
                     /translation="MMKKLINKKETFLTDMLEGFLIAHPELDLIANTVIVKKAKKEQG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDNGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEDINVETVIVRDDIAVTNEAQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDINHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     SVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            5336..5920
                     /gene="dhaL"
                     /locus_tag="NBI42_002269"
     CDS             5336..5920
                     /gene="dhaL"
                     /locus_tag="NBI42_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCD1778661.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVAKDDMHNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDND"
     gene            5913..6275
                     /locus_tag="NBI42_002270"
     CDS             5913..6275
                     /locus_tag="NBI42_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCD1778662.1"
                     /translation="MTKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNYRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
BASE COUNT         2108 a          881 c         1273 g         2061 t
ORIGIN      
        1 aagctattct tcatataaat tagttaaata gtaaatcttt gttagaactc tttccataga
       61 gttgtgacag tatttttgtt gtgctactat ttttatagtc taataaatat ataaagggga
      121 tggtttcgtg aataaaacgg ttaaagattt aatactagtt gtcttaggtt catttatctt
      181 tgctgcaggt gtaaatgcat ttattatttc tggtaactta ggtgaaggcg gggttacagg
      241 tttagcaatt attttatatt atgcgtttca tatttcacca gccatcacta acttcttggt
      301 caacgcagta ttgattgcta taggttataa atttttgagt aagagaagta tgtacttaac
      361 tattcttgta acaattctta tttcaatatt tttgagttta acagaatcat ggcaagtaga
      421 aactggaaac agcattgtga atgccatttt tggtggtgta agtgttggac taggaatcgg
      481 agtaattatc cttgcaggcg gtacaacagc aggtacaaca attttggcga gaattgcaac
      541 gaaatacctc gatgtaagca cgccatatgc tttgcttttc ttcgatatga tcgttgttgc
      601 aatttcactt acagttattc cacttgataa agtattagta acagtaatat cactttatat
      661 aggaacaaaa gtgatggaat atgtcataga aggtttaaac actaaaaaag ctatgacgat
      721 tatttcaact aatcccgaca aacttgccaa agcaatagac gagcaaattg gaagaggttt
      781 aaccatttta aacggacatg gctattatac gcgtgaagaa aaagatgtct tatacgttgt
      841 tatttctaaa acacaagttt caaaagtaaa gcgattaatt aaacaaatcg ataaagatgc
      901 attcctcgta attcatgatg taagagatgt ctatggtaat ggctttcttg cagatgaata
      961 aataaatggt atgagcacac atacttaaat agaagtccac ggacaagttt ttgaattatg
     1021 aagacttatc tgtgggcgtt ttttatttta taaaagtaat atacaagaca tgacaaatcg
     1081 agctatccaa tttaaaaagt aatgttagtc aataagattg aaaaatgtta taatgatgtt
     1141 catgataatc attatcaatt ggggtgcctt tgaaaattga taatttaaaa ataaaaatta
     1201 ttttttataa acagaaagaa ttttattgaa agtagggaaa ttatgaatcg tttgcatgga
     1261 caacaagtta aaattggtta tggggataac acgattataa ataaattaga tgttgaaata
     1321 ccagatggca aagtgacgtc aatcattggt cctaacggct gcgggaaatc tactttgcta
     1381 aaggcattgt cacgtttatt ggcagttaaa gaaggcgaag tatttttaga tggtgaaaat
     1441 attcatacac aatctacgaa agagattgca aaaaaaatag ctattttacc tcaatcaccc
     1501 gaagtagcag atggcttaac tgttggagaa ttagtttcat atggtcgttt tccacatcaa
     1561 aaaggatttg gtagattaac tgctgaggat aagaaagaaa ttgattgggc aatggaagtt
     1621 acaggaactg ataaattccg acaccgttca atcaatgatt taagtggtgg tcaaagacaa
     1681 cgtgtttgga ttgcaatggc attagcacaa agaactgata ttatcttttt agacgaacca
     1741 acaacatatt tagatatctg tcatcaatta gaaatactag aattagttca gaagctaaat
     1801 caggaacaag gttgtacaat tgtcatggtt cttcatgata tcaaccaagc gattcgtttc
     1861 tcagaccatc ttattgcgat gaaagaaggc gatatcatcg ctacaggttc aacagaagac
     1921 gtattaacac aggaaatttt agaaaaagtt ttcaatattg atgttgtttt aagtaaagat
     1981 cctaaaactg gaaaaccgtt actggtaact tatgacttat gtcgcagagc ttattcttaa
     2041 ttaagtaagt taatatgata aaaaggacaa ttaacatgac aaatagagag aatccaacgc
     2101 cattgaagtt tttatcctat attataggtt taagtatgat actactaatc acactattta
     2161 tttctacatt aataggtgac gccaaaattc aagcctctac aattatagag gctattttta
     2221 attacaatcc tagcaatcaa cagcaaaaca tcatcaatga gattaggatt cccagaaata
     2281 tagcagcagt aattgtgggt atggcgcttg cagtttctgg tgcgattata caaggtgtta
     2341 ctcgtaatgg tcttgctgat ccggcgctca taggtttgaa ttcaggtgct tcatttgctt
     2401 tagcattaac atatgcagtt ttaccaaaca cttcattttt aatattgatg tttgctggat
     2461 ttttaggtgc tattctagga ggtgctattg tattaatgat aggccgatct agacgtgatg
     2521 gatttaatcc gatgcgtatt attttagcgg gtgcagcagt aagtgctatg ttaacagcgc
     2581 taagtcaagg tattgcatta gcttttagac taaatcaaac agtaacattt tggactgctg
     2641 gaggcgtttc aggcacaaca tggtcacatc ttaagtgggc aattccatta attggtattg
     2701 cgttattcat tatattaaca attagtaaac aacttaccat tttaaatctt ggtgaatcat
     2761 tagctaaagg tttaggtcaa aatgtaacaa tgatcagagg catatgttta attattgcta
     2821 tgattctagc aggtatagca gttgctatcg ctggacaagt tgcatttgta ggtttgatgg
     2881 tacctcatat agcaagattt ttaattggaa ctgattatgc taaaattcta ccattaacag
     2941 ccttgctagg tgggttactc gtgcttgttg ctgatgtgat agcacgatat ttaggagaag
     3001 cgcctgttgg tgcaatcatt tcatttatcg gtgttcctta ctttttatat ttagttaaaa
     3061 aaggaggacg ctcaatatga ttagttcaaa taataaacgc agacaatgga tagcactggc
     3121 tgtttttagc attctactat ttctaggttg tacttggagt attacctcag gtgaatacaa
     3181 catacctgta gaaagatttt tcaaaacttt aattggacaa ggtgatgcca ttgatgagtt
     3241 aatcttatta gatttcagat tacctcggat gatgattact attttggctg gcgcagcgct
     3301 tagtattagt ggtgcaatag tgcaaagtgt cacgaaaaat ccaatagctg aaccaggtat
     3361 attaggtatt aacgcaggcg gcggatttgc aatcgcatta tttattgcaa ttggtaaaat
     3421 taatgctgac aactttgttt atgtactgcc gttaataagt atactaggtg gtatcaccgc
     3481 tgcattgatt atttttattt tcagtcttaa taaaaatgaa ggtgttacac ctgcgagtat
     3541 ggtattaata ggtgtaggtt tacaaacagc attatatggt ggctcaatta caattatgtc
     3601 aaaatttgat gataagcaat ctgatttcat cgctgcttgg tttgcaggta atatttgggg
     3661 tgacgaatgg ccatttgtca ttgcattttt accgtgggtg ttgattatta ttccttactt
     3721 actatttaaa tcgaatacac taaatattat tcatacaggt gataatattg cacgaggtct
     3781 aggtgtaagg ttaagcagag aacgtttaat attattcttt atcgcagtga tgttatcatc
     3841 tgctgctgta gcagtagcag gttcaatttc gtttatcgga ttaatgggcc cgcatattgc
     3901 caaacgtatc gttggaccac gtcaccagtt gtttttacca attgccattt tagtaggggc
     3961 atgtttactt gttatagctg atacaattgg caaaattata ttacaaccag gtggggttcc
     4021 agcaggtatt gtcgtagcaa ttattggtgc accgtatttc ttatatttaa tgtacaaaac
     4081 gaaaaatgta tagtgtcaaa ggacacaact tattgctatg aaaggcactt tattataagg
     4141 cttttcatag cattttttat ttaatgagtc actcaagact atttattttt caataatgaa
     4201 tcattaagtt atcaagacgg tcttatcaaa aatatatttg ataacggtat caggttaatt
     4261 ctttatgata gcgcattcat ttattctgtt ttatactatg actgataata ccaaggaggt
     4321 acaacatgat gaaaaagtta atcaataaaa aagaaacatt tttaactgat atgcttgaag
     4381 gatttttaat tgcgcaccca gagttagatc tgattgctaa tacagttatt gtaaaaaaag
     4441 ctaaaaaaga acaaggtgta gcaatagtct ctggaggtgg aagcggacat gaacctgcgc
     4501 atgccggttt tgttgcagaa ggtatgctag atgcagcggt ttgtggcgaa gtatttacat
     4561 cacctacacc agataaaata ttagaagcta ttaaagcagt agataatggt gatggtgtat
     4621 tactagttgt aaaaaactat gcaggtgacg tgatgaattt cgaaatggca caagagcttg
     4681 cagaaatgga agatataaat gttgaaactg ttattgttcg tgacgacatt gctgtgacaa
     4741 acgaagcaca acgtcgtggt gttgcaggaa cggtgtttgt tcataagctt gctggttatc
     4801 ttgctgaaaa aggttattca ttaacagaga ttaaatcgcg tgtagaagcg ttgttacctg
     4861 aaattaaaag tattggtatg gcaattgaac caccgcttgt tccaactact ggaaaatatg
     4921 gctttgatat tgaagacgac aaaatggaaa tcggtattgg tatacatggt gaaaaaggta
     4981 ttcataggga agaagtaaag gatattaatc atattgttgg aacattgtta gacgaattgt
     5041 ataaagaagt tactgccaat gatgtcatat taatggtaaa tggtatgggt ggtacgccgt
     5101 tatctgaatt aaatatcgta actaaatata ttcaacaaaa tttggctgca agatcggtta
     5161 atgttgctaa atggtttgtt ggtgattata tgacatcttt agacatgcaa ggtttttcta
     5221 taactatcgt gcctaataaa ccagaatatt tggaagcatt tttagcacca acaacaagtc
     5281 aatactttaa ataagaagtg aatatgaata aatatacatt tatgaggtga cacaaatgaa
     5341 agtgaatgat atgaaagcac gtttattaaa tttagaagaa acgtttaaaa aacatgaatc
     5401 tgaattaact gaattagatc gagcaattgg tgatggtgac cacggggtta acatggttcg
     5461 tgggtttagt agtcttaaag acaaacttga tgatagctca atgcaatcat tgttcaaatc
     5521 gactggtatg gcattgatgt caaatgttgg gggtgcatca ggaccactgt atggctttag
     5581 ctttgttaaa atgtctgcag tcgccaaaga tgatatgcat aatcaagatt tcattacact
     5641 aattcaggca tttgccgaag cggttgaatc acgtggtaaa gttactttaa atgaaaagac
     5701 aatgtatgat gtagtagcgc gagcagcaga gaagcttaaa aatggtgaaa ctttaacatt
     5761 caatgattta cagcaattag cagataatac aaaagatatg gtagcaacga aaggtagagc
     5821 agcatatttt ggagaagaat caaaaggtta tatagatcca ggtgctcaaa gtatggttta
     5881 tattttaaac gctttgattg gagatgaaga taatgactaa aattatactt gttagccaca
     5941 gtaaagaaat tgcaagtggt acaaaatctt tgttaaagca aatggctggt gacgttgata
     6001 ttataccaat agggggatta ccagatggtt caattggaac ttcatttgat atcatccaag
     6061 aagttttgac taaattagag gatgatgcat tgtgttttta cgatattgga tcttcagaaa
     6121 tgaatgtaga tatggcaatt gaaatgtatg atgggaatta tcgtgtgtta aaagttgatg
     6181 caccaattgt tgaaggcagt tttatcgcag cagtaaaact gtcaatcggc ggttcaattg
     6241 atgatgcatt agcagaaatc aaacaatcat tttagttaaa atttactaat aatgaaaaat
     6301 gtaaaccttt ttcaaatgaa act
//
LOCUS       DAHHRR010000129         6184 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.129, whole genome shotgun sequence.
ACCESSION   DAHHRR010000129 DAHHRR010000000
VERSION     DAHHRR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.129"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            95..2083
                     /gene="tkt"
                     /locus_tag="NBI42_002271"
     CDS             95..2083
                     /gene="tkt"
                     /locus_tag="NBI42_002271"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCD1778663.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDNAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEDERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            2361..2603
                     /locus_tag="NBI42_002272"
     CDS             2361..2603
                     /locus_tag="NBI42_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCD1778664.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            2590..2688
                     /locus_tag="NBI42_002273"
     CDS             2590..2688
                     /locus_tag="NBI42_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778665.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            2782..3249
                     /locus_tag="NBI42_002274"
     CDS             2782..3249
                     /locus_tag="NBI42_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HCD1778666.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            3373..4494
                     /locus_tag="NBI42_002275"
     CDS             3373..4494
                     /locus_tag="NBI42_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCD1778667.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIMISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPA
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYNHITDKKLSDIQSKKIKNILENELRKED"
     gene            4498..>6184
                     /locus_tag="NBI42_002276"
     CDS             4498..>6184
                     /locus_tag="NBI42_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCD1778668.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIALLWQEIESFDDD
                     KIKGLLEVATQQIDKVIENIPLLQARSKEILAFVNESKETAIKEYEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEGIEAKLKLLQDITNLLNYIENREKIETKIANCK
                     KDISKTNNKILNLECDKRTIDKEKKMLEENGDLIESKISFIDKTRVLFNDINKYQQSY
                     LNIERLRTEGEQLGDELNNLIKGLEKVEDSIGNNESDYEKIIELNNAITNINNEINVI
                     KENEKAKAELDKLLGSKQELENQINEETSILKNLEIKLDRYDKSKLDLNDKESFISEI
                     KSAVKIGDQCPICGNEIQDLGHHIDFDSIAKRQNEIKEIEANIHAIKSNIAVHN"
BASE COUNT         2345 a          798 c         1140 g         1901 t
ORIGIN      
        1 acattataac ttgaaataag tattttactt atctttttat tttaaaataa gttataatgt
       61 atttgataaa attgaagaag ggaagataca caagatgttt aatgaaaaag atcaattagc
      121 tgttgatacg ctacgtgcac taagtatcga cacaatcgaa aaagcgaatt ctggtcatcc
      181 aggattacct atgggagctg ccccaatggc ttacactttg tggacacgtc atctgaattt
      241 taatccacaa tctaaagatt acttcaatag agaccgtttc gtattatcgg cagggcatgg
      301 ttcagcatta ttgtatagct tgttacatgt ttctggtagt ttagaattag aagaattaaa
      361 gcaatttaga caatggggtt ctaaaacacc aggccatcct gaatacagac atactgatgg
      421 tgtagaagtt actactggac cacttggaca aggttttgct atgtcagtag gattagcttt
      481 agcagaagat cacctagcag ggaaatttaa taaagaagga tataatgttg tagatcatta
      541 cacatatgta ttagcttctg acggtgattt aatggaaggt atttcgcatg aagcagcttc
      601 atttgctgga cataataaat taagtaaatt agttgtttta tacgattcaa atgatatttc
      661 attagatggc gaattaaaca aagctttttc tgaaaacaca aaagctcgtt ttgaagcata
      721 tggttggaat tacttactag ttaaagatgg taatgattta gaagaaattg ataatgcgat
      781 tactactgct aaatctcaag aaggaccaac aattatcgaa gttaaaacaa caatcggatt
      841 tggttcaccg aataaagcag gaactaatgg tgttcatggg gcacctttag gtgaagatga
      901 aagaaaatta acattcgaaa attacggttt agatcctgaa aaacgtttta atgtttcaga
      961 agaagtatac gaaattttcc aaaatactat gttaaaacgc gctaatgaag atgaatctca
     1021 atggaattca ttattagaaa aatatgcaga aacatatcct gaattagcag aagaatttaa
     1081 attagcgatt agtggtaaat tgcctaaaaa ttataaggat gaattaccac gttttgaact
     1141 tggtcataat ggtgcatctc gtgctgattc tggtactgtt attcaagcaa tcagtaaaac
     1201 tgtcccttca ttctttggtg gatcagcaga ccttgctggt tcaaacaaat ccaatgtaaa
     1261 tgatgcaaca gattatagtt ctgaaacacc tgaaggtaaa aatgtgtggt ttggtgtacg
     1321 tgaatttgct atgggtgctg ctgtaaatgg tatggctgca catggaggtt tacatccata
     1381 tggtgcaaca ttcttcgtat ttagtgatta tttaaaacca gcgttacgct tatcatcaat
     1441 tatgggatta aatgctacgt tcatcttcac acatgattca attgcagttg gtgaagatgg
     1501 tcctactcat gaaccaattg aacaattagc tggactaaga gccattccaa atatgaatgt
     1561 tatccgccct gctgatggta atgaaacaag agtagcatgg gaagttgcct tagaatctga
     1621 atctacacct acttcattag tattgacacg tcaaaactta ccggttttag atgtaccaga
     1681 agatgtagtt gaagaaggcg ttcgaaaagg tgcctataca gtttatggct ctgaagagac
     1741 accagaattc ctattattag cttcaggttc agaagttagt cttgcagttg aagctgctaa
     1801 agatcttgaa aaacaaggta aatcagtgcg tgttgtttca atgcctaact ggaatgcatt
     1861 tgaacaacaa tctgaagaat ataaagaatc agttattcca tcaagcgtaa caaagcgtgt
     1921 tgcgattgaa atggcttcac cgcttggatg gcataaatat gtaggtactg caggtaaagt
     1981 tattgctatt gacggctttg gcgcaagtgc acctggcgat ttagtagttg aaaaatatgg
     2041 ctttacaaaa gaaaatatct taaaccaagt tatgagctta taagaataat ttataaagcg
     2101 agtatgttta gaagtctagg atgcataatc ttaggcttct tttaaagtgt tgaaatttag
     2161 agtatagcac tcaaactaca tcataagtga taagttttga aaatatacta tttcagatta
     2221 atctttaaaa cctctgttat aacagcatga tttttgatat tatttttagt atcgatatta
     2281 aaatacttga ataaactagt tcttgaaata atgtgatgaa tttagtaaaa ttcagtagga
     2341 taaagaaagc gggtgaaaca atggcaactt ggttagcaat tatttttata gtagctgcat
     2401 taattttagg tttaattgga ggtttccttt tagctagaaa atatatgatg gactacctga
     2461 agaaaaaccc accaatcaac gaagaaatgc ttcgtatgat gatgatgcaa atgggtcaaa
     2521 aaccttctca gaagaaaatt aatcaaatga tgacgatgat gaataaaaat atggatcaaa
     2581 atatgaagag tgcgaaaaag taaattcgca attgatagag gctattttcc agatatggaa
     2641 atggcctctt tttataatca aattaataag aataaatatg tttattaaaa ttaaagttaa
     2701 caaaatgacg aatagactga gaaatgctat aattcatttt gtatgattta cagagagttt
     2761 atttaacgag aaggtgtctg cgtgctctat ttaatatttt caatcattgt agctttattt
     2821 atgggaacta tagttatagt tattcgtatg aaagctcaaa attatccggt aaatgagaaa
     2881 aaaatagttt tgccaccgtt ttttatggcg actggtgcat tgatgtacgt cgttccatat
     2941 tttaggctaa caggatcgga aatgctagaa gcctttataa ttggtttgct tttttctaca
     3001 gttctaattt ggacttctcg atttgaagtc aaaggtacag aaatttatat gaaaagatct
     3061 aaagcatttc cagttatttt gatttcatta cttatcattc gtactgtgat gaaaatattc
     3121 attagtaatg aaatagatcc tggagaatta ggcggcatgt tctttttatt agcattctgt
     3181 atgattgttc cttggagagc agcaatgcta tataaataca aaaaactaaa gaaaacatta
     3241 atcaattaat tacttttaaa accacttgtg atcgacttct aaatcagtca atgagtggtt
     3301 ttaattttac ttgaaaaggt gaaatgttta tattaaaata aaagcgaaca aatgttctat
     3361 atggaggcag taatgaaaat tatacataca gcagactggc acttagggaa aatattaaat
     3421 ggtaaacagc ttttagaaga tcaagcgtat attttagata tgttcgtaga aaaaatgaaa
     3481 gaagaagaac ctgatatcat tgtgatagct ggagatttat atgacacaac atatccaagt
     3541 aaagatgcaa tcatgttatt agaacaagcg attggaaagc taaatttaga actgcgtata
     3601 ccaataatta tgattagcgg aaatcacgat ggtaaagaga gattaaacta tggggcgagt
     3661 tggtttgaac ataatcagtt atttataaga acagatttta catcgattaa ttcgccaata
     3721 gagataaatg gggttaattt ttatacactc ccttatgcta ctgtgagcga aatgaaacac
     3781 tattttgaag atgacaccat tgaaacgcat caacagggaa ttacgcgctg tattgaaaca
     3841 atagcaccgg caattgatga agatgccgtc aatattttaa ttagtcatct gactgttcaa
     3901 ggtggaaaga catctgattc tgaaagacca ttaactattg gaacggttga atcagttcag
     3961 aaaggtgttt ttgatatatt tgattatgtc atgctaggtc acttgcatca tccatttagt
     4021 atagaagacg acaaaattaa atatagtggc tccttattgc agtattcatt ttcggaagcg
     4081 ggtcaagcta aagggtatag acgtgtaaca attaacgatg gcattattaa cgatgtattt
     4141 attcctctta agccgcttag acaattggaa attatctcgg gcgaatataa tgatgttatt
     4201 aatgaaaaag ttcatgtgaa aaataaagat aattatttac attttaaact taaaaatatg
     4261 tctcatatta ctgatccaat gatgagttta aaacaaattt atcctaatac tttagcgctg
     4321 acgaatgaaa cttttaatta caatgaagaa aataatgcta tagaaataag tgaaaaggat
     4381 gacatgtcaa tcatcgaaat gttttataac catataactg ataaaaaatt atcggatatc
     4441 caatctaaaa agataaaaaa tattttagaa aacgaattga gaaaggagga ttaatgaatg
     4501 aaaccattac atttaaagtt gaataatttc ggcccctttt taaaagaaga aattgatttt
     4561 tctaaaattg ataataatga attgttttta ataagtggta agactggatc tggtaaaaca
     4621 atgatttttg atgcaatgac ttatgccttg tttggtaaag catcaactga acaaagagaa
     4681 gaaaatgatt tgagaagtca tttcgctgat ggtaaacagc cgatgtcagt aacgtttgaa
     4741 tttcaattaa atcatcgaat ttataaagtg catagacaag gcccttatat caaagaaggt
     4801 aatacaacaa aaacgaacgc taaatttgat gtatttgaga tggtggatgg caagtatgaa
     4861 attagagaaa gtaaagtaat ttcaggtacc caattcatta ttgaattatt aggagtaaat
     4921 gcagatcaat tccgacaatt gtttattttg cctcaaggtg aattcaaacg ctttttaata
     4981 tcaaacagtc gtgaaaagca agggatatta agaacactgt ttgacagtga aaaatttgaa
     5041 gctatacgag aaatattaaa agaagaagta aaaaaagaaa aagctcaaat cgagaataga
     5101 tatcaacaaa ttgccctttt atggcaagaa attgaatcat ttgatgatga caaaataaaa
     5161 ggcttattag aggttgccac tcaacagata gacaaagtga ttgaaaatat accactttta
     5221 caagctaggt cgaaagaaat actagcattt gtaaatgaaa gtaaagaaac tgctattaaa
     5281 gaatatgaaa taatagaaaa gaaaacacta gaaaataata tattaaaaga taatattaat
     5341 caactcaaca aaaataaaat tgatttcgtt caattgaaag agcaacaacc tgaaatagag
     5401 ggaattgaag ctaagttaaa gttgttacaa gatattacaa acctattgaa ttatattgaa
     5461 aatagagaaa aaattgaaac taaaattgct aattgcaaaa aagatatttc taaaaccaat
     5521 aataaaatat tgaatcttga gtgtgataag cgaaccatag acaaagagaa aaaaatgtta
     5581 gaagaaaacg gagatttaat tgaaagtaaa atctctttta ttgataaaac tagagtatta
     5641 tttaacgata ttaataagta tcaacaaagt tatctgaata ttgaacgctt gagaactgag
     5701 ggtgaacaat taggtgatga attaaataat ctaattaaag gtttagaaaa ggtcgaagat
     5761 tcaataggta ataatgaaag tgattacgag aaaattatcg aactaaataa tgcgataact
     5821 aacataaata atgaaattaa tgtaattaaa gaaaatgaaa aagctaaagc tgaattagat
     5881 aaactattag gtagtaagca agagttagag aatcaaatta atgaagaaac atctatattg
     5941 aagaatctcg aaattaaatt agatcgctac gataaatcga aattggactt aaatgataaa
     6001 gaaagcttta taagtgaaat taaatctgct gtaaagattg gagatcaatg tccgatatgt
     6061 ggtaatgaaa ttcaggattt agggcatcat attgattttg acagtattgc taaacgtcaa
     6121 aatgaaatta aagaaattga agcaaatatc cacgctataa aatcgaatat tgctgtgcat
     6181 aatt
//
LOCUS       DAHHRR010000130         6160 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.130, whole genome shotgun sequence.
ACCESSION   DAHHRR010000130 DAHHRR010000000
VERSION     DAHHRR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.130"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..141)
                     /gene="icaR"
                     /locus_tag="NBI42_002277"
     CDS             complement(<1..141)
                     /gene="icaR"
                     /locus_tag="NBI42_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCD1778669.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSI"
     gene            305..1543
                     /gene="pgaC"
                     /locus_tag="NBI42_002278"
     CDS             305..1543
                     /gene="pgaC"
                     /locus_tag="NBI42_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCD1778670.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            1507..1812
                     /gene="icaD"
                     /locus_tag="NBI42_002279"
     CDS             1507..1812
                     /gene="icaD"
                     /locus_tag="NBI42_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HCD1778671.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWVYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            1809..2681
                     /gene="icaB"
                     /locus_tag="NBI42_002280"
     CDS             1809..2681
                     /gene="icaB"
                     /locus_tag="NBI42_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCD1778672.1"
                     /translation="MKYRKLIILVLSILIILPVSTLDGHHIANADDDPPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            2668..3720
                     /gene="icaC"
                     /locus_tag="NBI42_002281"
     CDS             2668..3720
                     /gene="icaC"
                     /locus_tag="NBI42_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HCD1778673.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENLEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMIFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(4026..6068)
                     /gene="lip1"
                     /locus_tag="NBI42_002282"
     CDS             complement(4026..6068)
                     /gene="lip1"
                     /locus_tag="NBI42_002282"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HCD1778674.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRGNANYQRENGVDEQQHTENLTKNLHNDKTISEKNHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRTKEALPLNKSNQTSQREIVNKTEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKGDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNNDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTNATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKVTDTKQA"
BASE COUNT         1910 a          998 c          953 g         2299 t
ORIGIN      
        1 aatacttttt ttcgagtcaa aatggtaata taaactcgct ttttttatat ttacactttt
       61 agctatatca tcaagtgttg taccgtcata ccccttctct gaaaataagg ttattgcgtt
      121 gtcaataatc ttatccttca atttttataa ccccctactg aaaattaatc acactatgtt
      181 acaggaaaat taagttgcaa ttacaaatat ttccgtttaa ttataacaac aatctattgc
      241 aaattaaaat actatcaatt accatatggc ttacaaccta actaacgaaa ggtaggtaaa
      301 gaaattgcaa ttttttaact ttttgctttt ttatcctgta tttatgtcta tttactggat
      361 tgtcggttca atttatttct attttaccag agaaattaga tattcattga acaagaagcc
      421 tgacataaat gtggatgaat tagaaggcat tacattttta cttgcctgtt ataacgaaag
      481 tgaaacgatt gaagatacgt tgtctaatgt tcttgcactc aaatacgaga aaaaagaaat
      541 tattatcatt aatgatggaa gttcagataa tacagcagaa ctcatctata aaattaaaga
      601 aaataatgac tttattttcg tcgatttaca agaaaacaga ggtaaagcca acgcactcaa
      661 tcaaggcatt aaacaggctt catatgatta tgtaatgtgc ttggatgcag atactatcgt
      721 tgatcaagat gcaccatatt atatgattga gaatttcaaa catgatccaa aacttggtgc
      781 agttacaggt aatcctagaa ttcgaaataa gagttctatt ttaggtaaaa ttcaaacgat
      841 agaatatgca agtttaattg gctgtattaa gcgaagtcag acacttgctg gcgcagtcaa
      901 tactatttcg ggtgtcttca ctctatttaa aaaaagtgca gttgtcgacg ttggctactg
      961 ggatactgat atgattaccg aagatattgc agtttcttgg aaattgcatt tacgtggata
     1021 tcgtattaag tatgaaccgc ttgccatgtg ttggatgttg gttccagaaa cattgggagg
     1081 tctttggaag caacgcgtga gatgggctca agggggacac gaagtattac tacgagactt
     1141 ttttagcaca atgaaaacga aaaggtttcc tttatatatt ttgatgtttg agcaaatcat
     1201 ctcaatttta tgggtatata tagtgcttct atatttaggc tatttgttca taacagcaaa
     1261 cttcttagac tatacattta tgacatatag tttttcaata tttctactat catcatttac
     1321 tatgactttt ataaacgtta ttcaatttac agtcgcactc tttattgata gtcgctacga
     1381 gaaaaagaat atggctggac tcatatttgt aagttggtat ccgacagtat actggattat
     1441 taacgcagca gtagttcttg tcgcatttcc aaaagcatta aaacgtaaga aaggtggtta
     1501 cgcaacatgg tcaagcccag acagagggaa tacccaacgc taaaatcatc gctaaatatt
     1561 gtaagagaaa cagcacttat cgctatatcg tgtgtctttt gggtatattg tttagttgtt
     1621 ctactcgttt atattggtac tatatttgaa attcacgacg aaagtatcaa tacaatacgt
     1681 gttgctttaa acattgaaaa tactgaaatt ttagatatat ttgaaactat gggcattttc
     1741 gcgattatca tttttgtatt ttttacaatt agcatattga ttcaaaaatg gcagagagga
     1801 agagaatcgt gaagtataga aaattaataa ttttagtgtt gagtatcttg atcatattgc
     1861 ctgtaagcac actggatggt catcatattg caaatgcaga tgacgatcca cctaaaaaac
     1921 tgaaatataa agaaaatagt gctctggcat taaattatca ccgtgtaaga aaagcgaatt
     1981 ttctgaataa ttttatttac ttcttttcta gtagtaaaga aattaaaaat tatagtgtta
     2041 gccaatcaca atttgaatct caaataaaat ggctaaaatc acatgatgct aaatttttaa
     2101 ccttgaaaga atttttatat tacaagaaaa aaggtaagtt tccaaaacga agtgtatgga
     2161 ttaactttga tgatatggat gaaactattt atgaaaatgc ttatccaatc ttaaaaaaat
     2221 ataaaatacc ggcaactggg tttattatca caggtcatgt tggggaagaa aactttcaca
     2281 acctcgatat gattagtaaa aaagaactaa aagaaatgta taaaactggt ttatgggaat
     2341 ttgaaacaca tacccacgat ttgcataact tatctaaaaa taataagtca aaattaatga
     2401 aagcttctga agctacaatc ataaaagatt taaacaaaag tgaaaaatat ctaactaaaa
     2461 actttaaaaa gtcgcagaaa actatagcct atccttatgg cttgatgaat gacgataaat
     2521 taccggtaat caaaaaagct gggttaaaat acggtttttc attagaggaa aaagcagtca
     2581 ctccgaactc caatgattat tacatcccta gaatattaat tagtgatgat gcttttgagc
     2641 atttaattaa gagatgggac ggattccatg aaaaagatta gacttgaact cgtatattta
     2701 cgtgctatta tatgtgcaat tattattatc acacatttac ttacacaaat tactttaaaa
     2761 catgaaaatt tggagggagg atccttagta ttacaatttt acattcgtaa tattgtgatt
     2821 tttggtacac cttgctttat tatcttgtca cagttattga caaccttgaa ttaccaaaaa
     2881 gtcacctatc gatacttaac tacacgcgta aaatatatac ttattcctta catattaatg
     2941 ggattgtttt acagttatag tgaatcatta ttaacagatt caagtttcaa taaacaattc
     3001 attgaaaatg tcctattagg tcaatggtat ggctatttta tcgttgttat catgcaattc
     3061 tttattttga gttatatcat ttttaaaatt aactataacc tattcaatag taaaatatta
     3121 ttattgttat cttttatttt acagcaatca tttttatatt actttacgaa taacacagcg
     3181 tttcacgata ccgtgctaca ctattatcca ttaagtgaaa acactataat attcggatgg
     3241 attttttatt tctttttagg tgcatatatg ggttataact acgaacgtgt attaaatttc
     3301 ttagaacgtt atttagttat tatgattgta ttagctgtag ctacttattt tgtgtttatt
     3361 gcgttagcaa atggagacta ttggaacgtt accagctttt catattcatt aacaccatat
     3421 aatagtatta tgtttattgt tatcttgggt atttgcacgc attttaaaac aatgttattt
     3481 aatacgattc aaatgattag tgctttctca ttctttattt atttattaca tccaatcatt
     3541 ctagactcat tgtttgcata tacaaatata tttgaggata atacaatgat ctttctagcg
     3601 atatcactac tattcatttt aggattatgt ataggtgtcg gtatgatatt gcgtgaattc
     3661 tatatcttta ggtttattat tggaaaacaa ccatataaat tgaacattaa tgcttattaa
     3721 ttattaagct atgttaaaaa cacgcggtgg gcgaaatcag tttgaattga ctgacttcgt
     3781 tttaccgcgt gtttaatatt gttatacata tattctaatt gcacattatt gtaaggtgac
     3841 tttccgccag cttctatgtt ggggccccgc caacttacat tgtctgtaga atttcttttc
     3901 gaaattctct ttgttggggc cccgccaaga tattacttga ataattcttt tagaattcta
     3961 aataatgatc cgattaattg aaagaagtct gctgtcattg tatatacctc cctttacttt
     4021 ataaattatg cttgcttagt atcagtcacc ttttcagttt tcactaaatc gtctgctaaa
     4081 tgatgccaaa aatcttgtaa ttcttctctt gtgcgcactg tatcagaact atcttgtccg
     4141 acaaagtcaa catgatccca atcatgtttt gtaggcgtta cttgccaaat gcctttttga
     4201 attttatccg tcgcatttgt ataagcttga ttaaatggat gttgagaaga aataacggat
     4261 actaaaccat cgttttctcg ccattctttt tcagtagctt taccgattaa gttaccagta
     4321 atcacaaatg ggaaaaacat atttaagtct gctttttgtc tatcgctatt taatgctttg
     4381 tgcgttgctt caccagtgta tgttttatac acaatgttag ggttcaacga cgttttacga
     4441 tttaaatctg ttgcaccctc acgcgtcaga tcgtaaaatc cattatcttt tgatttccat
     4501 aaattagatt gtttaacacg tttgacataa tcaatatatg attcatttgg cttctgtttt
     4561 agaccccatt gagccaaccc gaagtctact cttgaatttt tattaccaaa cattttaccg
     4621 atatcaaata caatttgtct cactaaagct tcattaccag ctaaatctga tgcgtgtgta
     4681 ccattatgtg gtgttcctaa agtagtaatt gatgaaatca tattgtcatt attccctttg
     4741 aataatggag aaatttcgcc accatgtttc ttttgatact ctatttcttc acgattacca
     4801 ttacgcagta attcttctag ttgacgtatc gtttgaccgc ccatactatg tccaactagg
     4861 tgtaccttct gtcctggttt ccagtctttg taaattcctt cgtatgtttt tccataacgt
     4921 tcatgtccat attttgctgc atgtgctgca ccataatcta cacgaccgcc tttgatataa
     4981 taataaagtt caactgcgcg gtcatagtta cttccaaaag cacttatact tgcttcataa
     5041 gctttgtaac cattttcttc taaatcttgg cgaatgttca ttttattacc gccccaataa
     5101 tgagctaaca ctgaaggatt aatatcatct gtaaacccat tgaaaccatg cactaaaacg
     5161 ataggatctt gatttttata ctgcccttgt ttagcaactt tattcgtttg atcacctttc
     5221 gcttttgcag taagttcctt tgtccctttg tttggtaacg ttgctactgc actacttttt
     5281 aataaattta atccattttt cttgtcattg tcctttgttg gttctactgg tgatttttga
     5341 ttgtttttta aatctgaaac tgatagtgtt ttttcgtctt tttgaggttt cacttcttgt
     5401 tcattgttaa aattgtagtt agtaatttta tcattcgctt gattattttg ttgtggttgt
     5461 actttttcta tttctgtctt attaacaatt tctcgttggc ttgtttgatt tgacttattt
     5521 aatggtagtg cttcctttgt tcgactttgt gaaggtagat tgttattagc gtttgaatct
     5581 tgactattat ctgcctcttt tgactgttgc tgtgacgcca ctttactttg tttgccatca
     5641 ttaatagcct gttctaaccc ttgatttaca tcgctaggat tagcattgtt attttttgtt
     5701 gtatcacgtt gaatattttt attattaagc gatgattttt tatcatcctt tagttgatct
     5761 ttattcaaat catctgtttt acgatgattt ttttctgata ttgttttatc attatgcaag
     5821 ttcttagtta aattttcagt atgttgctgt tcgtcaacac cgttttcacg ttgataatta
     5881 gcatttcccc tagtttgttg atccccaata gattgtgctg taactgtatc ctcctgtgaa
     5941 tttcccatat tcacttgctt ctcagctgct tgtgcttgtc caccacttaa aaatagtaat
     6001 gtagctatta aaatggaaga tgcacctaca ctaaatttac gaatactata cttattttga
     6061 cttttcatca ttgtcagcac ctctattttt ttattttaga taaacaaatt gataattatt
     6121 cgaggaataa actcaaataa tttacttaac caagcactca
//
LOCUS       DAHHRR010000131         6156 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.131, whole genome shotgun sequence.
ACCESSION   DAHHRR010000131 DAHHRR010000000
VERSION     DAHHRR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.131"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(251..1129)
                     /locus_tag="NBI42_002283"
     CDS             complement(251..1129)
                     /locus_tag="NBI42_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HCD1778675.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGVPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(1149..4589)
                     /locus_tag="NBI42_002284"
     CDS             complement(1149..4589)
                     /locus_tag="NBI42_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCD1778676.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHIQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETTEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGLFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLVDRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDISFSTIEDFINSDLEKLKSHLNKNQ
                     LAFVHAVEKHYNLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHIQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTVYK
                     DKQRLQHQLIDATIQSTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIVNPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQLMLL
                     RQQLEDYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4893..>6156
                     /locus_tag="NBI42_002285"
     CDS             4893..>6156
                     /locus_tag="NBI42_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD1778677.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQIERQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYA
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWTIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGDL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSSHHGNAAMPQTKLDYQISIDKVV
                     EQVQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFISWIKRTKN
                     TAVKVPHRVKKPASISLIICLIVLGLLMP"
BASE COUNT         1921 a          994 c          922 g         2319 t
ORIGIN      
        1 taaataatca aatccatttt tttaatgaat aaagttgtaa ctgtactatt ttgtagtgta
       61 tggtaattta tttggatgaa atataattct ttaaactcta ttgattaatc aaatatgtat
      121 caatcaaaat tcggcttatt tattcacttt gacatatcca aatacgcaaa aagactattt
      181 ctaccttgta tcgatagaaa tagtcttttt atacttaatt ggtttaattt ttcataaaat
      241 cttatactct ttaaaaatgg gatgaaatat attttcctga aacatgtaat tcatgttcgt
      301 tacaaattga taaaatatga ttaagcgttg ttgcaaatga cattttctca aatagcgctt
      361 cactatcaat tggtacttgt gtgtgaattt cagcaagtcg cttcgataaa tatagttcat
      421 ctaaattgtc attaatctta ttacgttgcc cagctgataa tgcatcaatg ttttcaacca
      481 cattttcaac gctttgatat tgctgaatta acttaatagc cgttttctca ccaattcctt
      541 taacacctgc atatccatct gctgtatcac ccataaacgc tttaatatcg attagttgtt
      601 gtggttcaag ggcatattct tcgttaaaac gatgtaatgt atatctatta taaatgttaa
      661 aacctttttt aattagccaa acttcaacat tgtcattaat acattgcagt aaatctttgt
      721 cgcccgtaat aatatataca tcgttatcag ttgaatattg ttgtgctaat gtacctataa
      781 catcatccgc ttcatagttt ttaacgccaa tatttacaaa gccaaattgc tctgaaattt
      841 ctttaacata atcaaattgt ggtatcaatt cttctggtgg tgcagaacga ttttgcttat
      901 aaccatcaaa catatcattt ctaaaagttg attgtcccat atcccaacat acagctacat
      961 gtgtagggcg tatttcatgt attgccgaaa agatatgacg tacaaagcct tgtatcccat
     1021 ttgtaggtac accttgtgaa ttgtacataa attgtttatg aagacttgta gcgtagaaat
     1081 gtctaaataa tagcgccata ccatctacaa gtaatatttt attaggcatt taaaacgttc
     1141 cttctttctt aatataaaat agttttcagt tgttgatgtt tatctttcaa ttgattgata
     1201 agtgttgtat ctaaatcaaa tgctaataaa tcgtttattt gcgtttgaat attagcttca
     1261 aaactttcaa attgcgattc agcttctaca gccatttgtt taacgtaatc ttcaagttgc
     1321 tgtcttaata gcatcaactg tggttgtaat aatcctaacg tactttgaca aattgattca
     1381 tgtatatctc tttgcccatt tggattcaaa attttacgtt ttgttaattg tttaggcaat
     1441 gcatttagca tatcgttgaa atcgatgtgc aataatggtt gctctatatt agctgattca
     1501 aagttaaact gaggattaac aatgacatgt aaatctgcta attgttgaac gattggtgca
     1561 atttgttcag tgagttgctt attaaagtat ttttttatac gttctgtaat taaagattgc
     1621 tctaaaaaca atcgttgatg aatttgatct aagtatactc tcgtagacac cttcttttct
     1681 tcattaaaat cactattttg cgtcatttga gaattaaaca ctgatttaac atcgtcaagt
     1741 agttgtagtt ttaaacgggc atttaagtga taaacttgct cttcaacttc gttgtctgta
     1801 ctttgtatag ttgcatcgat taattgatgt tgtaaacgct gtttatcttt atagactgtt
     1861 aacctttgtt ggcgacgtga aatatcagct ttatttgttt caaattctgt aatcatctct
     1921 acataagaac gatccatttg ttgaagctga tgaatcattt gttgttctaa aattgattta
     1981 gattcaacat caacaaattg ttgtatgctt tgttttaatt gatcaatacc cttatcttca
     2041 gcttgcaatg catttcgact tgatacagca aaaatgtctg attgtatgtg tacttgtctt
     2101 aatgcatctg atacatatgt ttcaaccgct tcaagatcat cttgactttc tgctaaatca
     2161 gcagcattaa ttaccatttt aaatgcttgg ttttcattca actggttcat atctttcatg
     2221 tgttctataa acgctttgtc attatcagta aatgaatgat taaaataact tacatacaat
     2281 attaagtctg aagaagttaa aatttgctcg gtttcatttg tatgcctttg gttatttgag
     2341 tgtaacccta atgaatcaac aattatttta ccttttaacc aatcatgcat taaagcaatg
     2401 tgtactgttt taacaaatgt tgcgtattca tcttctgcac tccacttttt caattcttgt
     2461 tgattaatgg catgtttttc accattttct aacatattga catacaaatt ataatgtttt
     2521 tcaactgcat gaacaaaagc aagttggttt ttatttaaat gtgattttaa cttttctaaa
     2581 tctgaattaa taaagtcttc tatagttgaa aaagatatat cttggtattc aactactgca
     2641 ttaatttcat ctaataattg cgattgtgat tttagcgtta tataactctc gtctccataa
     2701 gatatttctg tagtagctgc tgtagtagga tttggagaac tgactaaaat atgctcgcct
     2761 aataatgcat ttatcaaact acttttaccc gcactaaatg ttccaaatac acctattttt
     2821 attaatttat tatctatacg tgttaatgtt tcgtggatat cctgctttgt acgcttgaac
     2881 aaaggcacat ccgaaattat atcaagtgct ttttgaatat ctatagacat atttgttgta
     2941 gctgtatttt gatttagctg ctgattatca cgattatcct gagcattatc agtagccact
     3001 tgatatgttg tctcttgtcg atctactaat ttatctagag attcatctaa atgaatatag
     3061 tagtgacgat aattcttagt cgtcaatgac tgacgcagct catttaattc tgtataacgt
     3121 tgatattctt ttaaatcatc actttcttct gttggtaatt catctgcctg cacattttct
     3181 attatttctt taaaaatcgg tgttgattgt tgttcaacat atttcttaat ggctttaacc
     3241 acttcgtctg aaaatgtaag tacataagta ttgctgattg atgtttgtgg ttgatataaa
     3301 ccttcaatca tttccggctt aacgtcataa tgctgattta atactttatc tgaagcttct
     3361 tttttattga taaaacgcgt aacaaaagac atatcttctc gcattggttg acgaatttgt
     3421 tgattaacat gttcttgtaa cgcatctgtc gctgttaata atcgctgttg ttgaatttgt
     3481 agctttttct tctttttatt aaaaaggccg ccaacattaa agtcttgaga catactttcc
     3541 aaataacttc gtaacatttc tctcatatta tgcggcataa tgtatgcatt ttctaaaata
     3601 tttttacgct tattctttaa aaatgccatt aattcatctg gattatttaa aagttgtgcc
     3661 tcttcactaa ttgcttgatg ttgttgacta tttaaaaatg cttgttcaaa ctctgcttct
     3721 tcgataccta aatcttctag tacttcctga atttcagact gaatgtaatc tagctgagct
     3781 tcggtaatat attcaaccgt tcttgatgta taatcctctg ttgtctctct atgttgatct
     3841 aatgaaatta gataacttga taatacttca agttcatttt cagggtgatc aaatttcgat
     3901 acataaaagg tgcgttctaa tttaatgccc caatctgcaa ttgatttttc aactcgtgat
     3961 ttaaacgtag agaatgacaa ttcatcgtct tgatgcttgt caatttgatt aatgataaac
     4021 acaacaggta ttccaacatc atttatatgc ttcataaact taaagttaag ttcagattga
     4081 atgtggttat agtcaaccgt ataaaatatc atattacttg tatacatata ttgttctgtt
     4141 attgactgat gtgatgcaac atttgaatca acacctggtg tatcttgcaa cgtaaaccca
     4201 ttttcaaatt tagctgattg aaaattaatt tctacagatt caacgtcgac attttggcga
     4261 ttcatttccc ttacttcatc ataattagat aatttggcat atgtttgatt cggcaaatta
     4321 gcaataatat cgtgattgtc tgaaactgac acgatagcag tattacttgt cgttggtaca
     4381 ggtgaacttg gtaagatatc ttgttcaatt aataaattta tcagtgtcga tttacctgca
     4441 gaaaaatgtc caacgaacga acatgtatat tgctgcaaat atactttctt aattacttgg
     4501 ttaattgtat gtaaaagtgc ttcatttcgc gacttttcaa cttctttttt taatttatat
     4561 aaaagatcta attgttcttt attaatcatg ttactttccc ctttggttcg ttcacataga
     4621 aatgcttgct tgttaaaacg aattcataca atatcgatgt ccatttaaac ataaatcgtt
     4681 aaatggctcg cattgtatta tgtttaaatt ttatcatagc tacttgataa aacatacgaa
     4741 taatcaattt tcttcaaaca ttctaactta aaagttcatt tttctaaaaa tcacaaatca
     4801 tcgttaaatt tttctagaaa ataaatattg tcacagtttg ttcacacacg tcacagaatt
     4861 gacaccgtat aatagccaat tgggagggaa atatgaaaaa tacatttaat ccattacaaa
     4921 gattacattt ctatgcagca atatttattg ctccactgtt aatcacttta accatttcgg
     4981 gcattggtta cttgttcttt ccagaagttg aaaataatat ttataagaat gagttttttg
     5041 gtgacagtga tgtcaaaacg catcaaacat taaatgatgc agtacatcaa attgaacgac
     5101 aatatgaagg attctttgta agtaaagtta gcatacttga tgaaccatat aacaaacgaa
     5161 ttacactgag tgatatggca ggaaatcagc gttacgcctt tctagatcat aacaatcaat
     5221 ttgttgcaga tcaaaatgcg aaacatacgt attctaatgt gatgcgaagt atacatagtt
     5281 ctttgtttac tgaaaatact attattaatt atttagttga gttaaccgca tgttggacga
     5341 tattcatgat tttatctggt acttatttac tcattaagaa gcatttaatt tctaacaaaa
     5401 gtaaggcact tcgttggcaa aagtggcacg caatgattgg agttatcatt gcaattccag
     5461 tgtttgtatt agttttaact ggattgccat ggtctggttt tatgggcagt aaaattgccg
     5521 gtatgatgga cacaaacggt gaccttggtc aaggtgaatt agcgattaat ccacctaaat
     5581 ccgacttgaa tgagttacct tgggccaccc gtaaaaataa acagccagct tcatccgaaa
     5641 aaggttcaag tagtcatcat ggtaatgcag caatgcctca aaccaaatta gattatcaaa
     5701 tatctattga taaggtcgtt gaacaggtgc aaaaagctgg tattaaaaag ccgttttcaa
     5761 tcgtatatcc aagtgataaa aatggtacct ttattgtatc taatactagt aattcaggtg
     5821 ttactgggct agatgtatca ccttacaagg aacaaacact ttatttcgac caatatagcg
     5881 gtaaaaagct aggtacgatt aaatatgatg actatggtat tattgctaaa tggtttacat
     5941 ggggcattcc gcttcacgaa ggtcatttat tcggtatttt aaacaaaatc attaatttat
     6001 ttgtatgtat cgctttatta gtagccattg gtatgggatt tatctcttgg ataaagcgta
     6061 caaaaaatac tgcagtaaaa gtaccacatc gcgtaaaaaa acctgcatct atatcactca
     6121 taatatgttt aattgtatta ggactattaa tgccat
//
LOCUS       DAHHRR010000132         6141 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.133, whole genome shotgun sequence.
ACCESSION   DAHHRR010000132 DAHHRR010000000
VERSION     DAHHRR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.133"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(53..1315)
                     /locus_tag="NBI42_002286"
     CDS             complement(53..1315)
                     /locus_tag="NBI42_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCD1778678.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVVLAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHNQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            1463..1648
                     /locus_tag="NBI42_002287"
     CDS             1463..1648
                     /locus_tag="NBI42_002287"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCD1778679.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            complement(1736..2719)
                     /locus_tag="NBI42_002288"
     CDS             complement(1736..2719)
                     /locus_tag="NBI42_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD1778680.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            2856..3365
                     /gene="msrA"
                     /locus_tag="NBI42_002289"
     CDS             2856..3365
                     /gene="msrA"
                     /locus_tag="NBI42_002289"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD1778681.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            complement(3568..6090)
                     /gene="mprF"
                     /locus_tag="NBI42_002290"
     CDS             complement(3568..6090)
                     /gene="mprF"
                     /locus_tag="NBI42_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCD1778682.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVVITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILN
                     KITWVRWVLYAVSLFLPLFIIYSMVRPPDKNNRYVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDVHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYLLLAIHTSACLLLLLNVVGIYK
                     QSRRAIIYAMISIILIIVATLFTYASYILITWLAIIFALLIVAFRRARRLKRPIRMRN
                     LVAMLLFSIFILYINHIFIAGTFYALDVYTIEMHTSVLKYYFWITILIIAIIVGAIAW
                     LFDYQFSKVRISSNIEECEEIIDQYGGNYLSHLIYSGDKQFFTNEDKNAFLMYRYKAS
                     SLVVLGDPIGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINEIQHVSDLWLDN
                     RQEMHFSVGQFNETYLSKAPIGVMRNENNEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
BASE COUNT         2147 a         1116 c          849 g         2029 t
ORIGIN      
        1 gagacttatt ttatacgaat cacagttata tattacttaa accgtatagt cattacattt
       61 tatgtccagc cattaaacct aatctgccgt gttttgtacc tgcttctgta aacgatactg
      121 ctttggacac aatacctttg ccgtatttca aatgtaattg gtctatcgtt ttagctagac
      181 attcgtcacg tttgcgttgg tattcatctt caaacagact taactgtcgc tcatcctcat
      241 taataaattg actcaaagat atacttagcg tacgatatag tgcttgttta ttacataatt
      301 tatctgcgaa atgcattact actttataaa tatctttttc taagtttgtt ggatctttca
      361 aagtatattg cttatgtaca ccgccttcat cactatagcc aaaggcaaaa tgtatcgttc
      421 ttgccacttt ttttcttgct cgaactctgc tagcaacatc ttcaattaac tcttgcatta
      481 ctacttttgc ttcatcaaaa tgataatctc tcattaatat ttgacttttg catatcgatg
      541 gattgctgat cttgtgcttt tcacgtactt tactctgatc tatcccattc gcatgtagat
      601 gcatatcaac acctaaaata ccgaactctt tttttaaaaa tttatatgga tattttgcta
      661 aatctcctat agtaaaaatc cctcttttat tcaatttggc ttctgttcga cgattaatac
      721 cccaaaaatc tcgcaagggc tgaattggcc ataattttgt tggtacatct tgatatcgcc
      781 attcagctat accattttga ttatgcttcg cttcaacatc catagcaatt ttacttaata
      841 acatattaga accaatgccc acagtacaat aaatgcctgt ttcttcataa atttcacgtt
      901 taagtctttc gcaaaatgca tgtactgtag aactaaatct atgataacta tcagtaacat
      961 ccataaaaaa ttcgtcaata ctatattggt gtaaatcttc aggtggaata taacgcaatg
     1021 caatcttaga aatagcaact gaaacattaa gatatttacg catacttgga ttgataatgt
     1081 aaatatcatt tctatgtggt atttcaaaca atcgcgatcc tgtcttgatg cctaattctt
     1141 ttaatttagg tgtcgcagcc aatactacag aaccctgacg cttagtatct gcaacaacag
     1201 ctaactttgt ttctaatgga tctagcccct tttcaataca agaaacactc gcaaaaaaac
     1261 ttttttggtc aatacataga acatccctat cttctaataa atgataatta tacactgtta
     1321 ttcctccctt acttgaattc cattatatac gaacacttgt tctatttcaa ctatttcaga
     1381 acaaatgttc tgttattttg caaatgaatt tttaagtatt acatgatata ctcaaattaa
     1441 taatgattag gaggtttgga tgatgccaat cgtcaatgta aaattattag aaggtcgttc
     1501 ggatgaacaa ttaaaaaatt tagttagcga agttactgac gccgtagaaa aaacaacggg
     1561 agcaaataga caagcaattc acgttgttat agaagaaatg aaaccaaacc attatggtgt
     1621 ggctggcgta agaaagtcag atcaataatt cttcataaga tgtatgccaa ttaattcttt
     1681 gaaaacgaac aaggcgactt ctatctgagt atgatagaaa tcgccttgtt tatttttaat
     1741 cttcatctaa aaagtcttta atagcttgtt tatttgtgtt tttattaatc tgtaatgcac
     1801 taccatcagt atttgtattg acatcttcgt atgagttctt gattggcaca gtcaatgact
     1861 taacatcttt ttcacctcgg ataccaaaac tcaaacctgt ttggaagatc cctgaatcag
     1921 gaatgtttgt attcacatag cctcttaaaa tacctgcaac ttttggtaat ttaacaactg
     1981 ttctaaaatt aaccatttct tttttcaatg tttgcatcac ttgttgctga cgtcgcacgc
     2041 gtccgaagtc accttcaggg tcgtgacgga atcttgcata accaagtaat tctttaccat
     2101 tcaacctatg gttacccttt ttcaaagata caccaatatt tttcgacata tctttttcga
     2161 cattaattgg tacaccttct ggcattaatt catcaatcat tttctcaaat ccagtaaaat
     2221 caactactgc ataatattca ggattaattc ctaaattttt atcaagtgtt tttctaagta
     2281 gctctggacc acctaaagcg tatgctgaat taattttgtg ttttccatat cctggaatat
     2341 ctgcataaat gtcacgcatg acagacatca ttttcatctt tttattgata aagtcatatt
     2401 gaacaaccat gatagaatct gttcttgatt gtccaccttg tgctttatct gcaccgagta
     2461 caagaataga aattttccca tcatttttta ctggtccatt aaattgatgt actttaacat
     2521 ctttcgcatg tttcttggca tattctacac cgctattgta actatgtaca atatatacaa
     2581 ctaatgcgat aagtaaaatt acaacaatca gaagaatgat aggtaatttc ctaattttct
     2641 tcttcttttt tcgcttaggc gccgaagtgc gatgttcact ttgacgtcta tattcgttgt
     2701 cattagtttc tttatccata tttacctacc ttatatcttc aaaaatagat ttttgtgtac
     2761 tataataatc acatattaac gttatataaa aacaattcaa aaagcaagtg atttcttaat
     2821 aaatcgtact aaggtctaat gaaaggatgt caaaaatgaa tattaataca gcttattttg
     2881 ccggaggttg tttttggtgt atgacgaaac catttgacac ctttgacggc atagaaaaag
     2941 taacttctgg atatatgggc ggacatattg aaaatcctac ttacgaacaa gtaaaatcag
     3001 gtacgagtgg tcatttagaa actgttgaaa ttcaatatga tgttgcatta ttctcataca
     3061 ataagttatt agaaatattt ttctcagtca ttgacccatt agatacaggt ggtcaatatc
     3121 aagaccgtgg tcctcaatat caaacagcta ttttctacac taatgatcat caaaaagaac
     3181 tcgctgagac ttatatcgag cagcttaaaa atacgattaa tgctgataag gcaattgcaa
     3241 caaaaatatt gccagcgtca caattttaca aagccgaaga ctatcaccaa gatttttata
     3301 agaaaaatcc agagcgctat gcagaagaac aaaaaatacg ccaagaatat aaaaataagc
     3361 aataacattt atacttaatg aaactgattg agctgataat aaaagttagg cgacttaact
     3421 taagctcatt tcaatattaa aatacggtac agataggaaa cttaaatact cttatctgta
     3481 ccgtttttta taaatcattc gttatagaaa ccaaaaaatc caagcgcttc aggcataact
     3541 gtataccttt tagcacttgg atttttatta tttgtgacgt attacacgca ttactttaga
     3601 aagtgattcc caaagcgaat tatctttacg ataaactaaa aaacgtggtt cccaatttgg
     3661 attatattta gatttataac gacgtaatcc ttggaaacga tatagaccgt tgaaatgttc
     3721 aaagacacgg cctgcaagtc gttctcttaa atatgaataa tgcaattgac caacatttga
     3781 taacgtcgcc atacccatat tgaatttcgt atagccttgc tctttactcc aaagtaacat
     3841 gtgcaagtat aatccatcca ttaacggtaa atctaactca ggcaaccatc taattaaatc
     3901 gactgaaatg gcatcattaa agtatgttgg cattaaacta caaaatgcaa ttacttcatt
     3961 attttcattg cgcatcacac caattggcgc tttagataaa tatgtttcat taaattgacc
     4021 aacagagaaa tgcatttcct gacgattatc taaccacaaa tcactgacat gttgaatttc
     4081 atttataaat tcagttgaaa acggtggttc aataatttcg aatgaaatat taagttcatc
     4141 gaatttgttt aaagtcgctc taaatccacg gcgtttttta cctgaagttg aaaattgcgt
     4201 taaatcaata attgcttctt cacctaattt gaaaaattgg ttaccgaaat tatgatataa
     4261 aggcatgtgt tgatctgtaa cttgatagaa tataacatca tagcctaaat actcagcgta
     4321 attatagaat gcttctaaca attcatcaaa ggcattttca tcaccgattg gatcgccaag
     4381 aactactaac gaacttgctt tatagcgata cattaaaaat gcatttttat cttcattagt
     4441 aaagaactgt ttatcaccac tataaattaa atgacttaaa taattcccac catattgatc
     4501 aataatctct tcgcactctt caatattaga agatatacgc actttactaa attgataatc
     4561 gaataaccac gcaatcgcac caacaataat tgcgattatt aaaatggtaa tccagaaata
     4621 atatttaagt acagacgtat gcatttcgat tgtataaaca tctaaagcat aaaatgttcc
     4681 agctataaat atatgattga tgtagagtat aaatatgctg aacaaaagca ttgcgactag
     4741 atttctcatt cgaattggac gttttaacct acgtgctcta cggaaagcaa caataagtaa
     4801 agcgaagata atagctaacc aagttattaa aatgtatgac gcataagtga ataacgtcgc
     4861 gacaataatt aagataatcg aaatcattgc ataaataatg gcacgtctac tttgtttata
     4921 aatacctaca acattaagta aaagtaataa acaggcactt gtatgtattg ccaataaaag
     4981 ataatacgtt aaatgattgc catcatataa agcatcgtat acaatcgtca aattatttac
     5041 aaagaaaatc atacttgtaa agaatactaa aattgccaat gataatgatg gtattttagc
     5101 aataatatct ttttgatacg acattaaaaa cgatgtcaca tctttagccg gaatgaaata
     5161 tttagatcct tcaatatatt tcttagctga agtaccaaac tcaaatgatg aaagaattaa
     5221 tgcaataatc actggtacga aatagtacgc aaagcgataa agtagtagca ttaataatac
     5281 tttttcttca ggtacaccta acgttttaaa gcctaataaa acaactaagt cgaaggcacc
     5341 aaaaccacct ggaataaagc tgactaatcc agataatgca gcaataatga atattgcaat
     5401 aaatgacata aatgagacat ggacgtcaac tatgacacca cagaagtata atacaactgc
     5461 agcggctaac cattccacac atgacactaa tgtacaatac aagcctacat aacgattgtt
     5521 tttatcgggc ggtctaacca ttgaatatat aataaataat ggtaagaaca acgacacagc
     5581 ataaagtacc catctcaccc acgtaatttt atttaaaatt aatgatgcat caaaaacatg
     5641 gaatacgatt aacaatgata acaaacttaa acctgtcaac attgaaatca gtattaatga
     5701 aataaagtga accaatttct ttttatcatg cgtatagttt ttgtaaacca ttgctcttac
     5761 accggcacca ataaagccac caaagcctac aatcgcattt aatgcattaa tgatataact
     5821 tactcttaaa acttttccta atgaaatatc catttttaaa gctctagata aaatcacatc
     5881 atacattgag agaataacaa gcgatgcccc accaccaata aatagtaaaa ctaaggacat
     5941 acggttaatc ttactaaatt caaccaacgt atctttaaag ttaataccag ataactcccg
     6001 atacaatgtg attactacaa aaataaataa agctgtagca aacgtaattt ttaagattga
     6061 aaatattttg tttttaactt cctgattcat tttttcacat caattctaat tatttctgtt
     6121 ataaatcaaa atatatcata t
//
LOCUS       DAHHRR010000133         6092 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.134, whole genome shotgun sequence.
ACCESSION   DAHHRR010000133 DAHHRR010000000
VERSION     DAHHRR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.134"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            13..204
                     /locus_tag="NBI42_002291"
     CDS             13..204
                     /locus_tag="NBI42_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HCD1778683.1"
                     /translation="MKQKKSKNIFWVFSILAVIFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(358..1185)
                     /locus_tag="NBI42_002292"
     CDS             complement(358..1185)
                     /locus_tag="NBI42_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCD1778684.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWVVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPVLGEIFKDVVNISRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILNE
                     VDGQASNLLGEPLTIGGPNSVLVGNPGVHREILDVYLEPHRDALIQLHEQRFKRKSK"
     gene            1342..1956
                     /locus_tag="NBI42_002293"
     CDS             1342..1956
                     /locus_tag="NBI42_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HCD1778685.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLHELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLKEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            2139..3491
                     /locus_tag="NBI42_002294"
     CDS             2139..3491
                     /locus_tag="NBI42_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCD1778686.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIIQTFQELQG
                     "
     gene            complement(3573..3992)
                     /locus_tag="NBI42_002295"
     CDS             complement(3573..3992)
                     /locus_tag="NBI42_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD1778687.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLHTTIGALAIILLISTLYAIFATICIKGRRKLSIILFVIAIIVSL
                     MALNILAIVLWIIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(4275..5294)
                     /locus_tag="NBI42_002296"
     CDS             complement(4275..5294)
                     /locus_tag="NBI42_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD1778688.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(5368..>6092)
                     /locus_tag="NBI42_002297"
     CDS             complement(5368..>6092)
                     /locus_tag="NBI42_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1778689.1"
                     /translation="DSDYMNMSFDKGNKYSLPYFFGTVGILYNKEKYPNESFDSWKSL
                     YNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVGD
                     EITMMLQQNEGNIAVVWSGVAAPLVQDGDKYNYVIPKEGSNLWFDNMVIPKTAQNKEG
                     AYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKKIKDDHRFYPTKKEQERLEVYK
                     DLGPEVLSEYNENFLNFKMSLK"
BASE COUNT         2021 a         1045 c          874 g         2152 t
ORIGIN      
        1 ggtggggata ggatgaaaca aaaaaaatct aaaaatatct tttgggtatt ttctatatta
       61 gctgttattt tcttagtatt atttagtttt gctgttggtg catcaaatgt accaatgatg
      121 attttaacat ttatattact cgttgcaacc tttggaattg gatttactac aaagaaaaaa
      181 tatcgagaaa acgattggct ataagttata tcgacaataa cacgcgttga ttaaagacca
      241 cacatgttca tgatgaacat ttaaatggtt ttatattggc gcgtgttttt attatttaag
      301 atataaaaac gtaatatgtc acctaattgt taggactaca tattacgctt ttggtaatta
      361 ttttgatttt cttttaaatc gttgttcatg taactgtatt aacgcatcgc gatgtggctc
      421 taaatataca tcgaggatct ctctatgaac accaggatta ccaactaaaa ctgagtttgg
      481 accaccaatt gttagcggtt cacctaataa attagaagct tgaccgtcca cctcatttaa
      541 aatgactagt cctccagcaa aatcccacgg ttggagtcta ggtgtcatat atgcttctaa
      601 attaccggta gcgacagaaa caatttcaag cgctgcacta ccatatgccc ttgcacttct
      661 tgaaatattt acgacatcct taaaaatctc tcctagcaca ggtttcgtca accagttcgg
      721 attgatccca ataatgcttt gtcttagatt agaatcattc aagggtttca agggttggct
      781 accacgatat gcaccttccc ctactttagc atgatataag acatcagcca taacatcata
      841 tacaaaacct gcataaggtt taccatcgat ataaatacca attgaaatcg cgaaattttc
      901 ttgttgatga acaaaattca atgtaccgtc tattgggtca acaacccata ccgtaccttt
      961 ggaagtatca atgtcatgac catgcccttc ttcacctaat acttgatgat tgggatatgt
     1021 ttctaaaatt gtatcaaaaa taaaatcttc tgttgcttta tctacatttg taacaaggtc
     1081 attcggattt gactttgttt caattgttaa attttgctcc atcaattgtt taattctaat
     1141 tcctgcttct tgaataagtc cttgggcaaa tccataaagt gccataacat catctcctaa
     1201 catctttatt atacatcaac attttataaa aataacttct taagataaaa atgaaaatac
     1261 taattttaat gagagcatct taagctgcta ttgataaaac atatgctaca atatttttaa
     1321 acactataca ggaggtactc tatgacaaaa tatacattta aacctaaaga tttcaaagcg
     1381 ttcaacgtag aaggcttaga cgcacgaatg gaagctttaa acgaatacat acgaccacaa
     1441 ctccatgaat taggagaata ttttagtgac ttctttacaa gtcaaactgg tgaaacattt
     1501 tatcctcacg tagcaaagca tgctagaaga agtgtgaatc ctcctaaaga tacatgggtt
     1561 gcttttgcaa caaacaaaag aggctataaa atgttacctc atttccaaat tggtatgttt
     1621 gaagatcaac tgtttgttat gtttggaatc atgcatgaag caaaagataa agcaacacgt
     1681 gcaaaagttt ttgaaagaaa atttaaagct attcaacaat tacctgatga ttatcgtgtt
     1741 tgcttagatc atatgaaacc tgataaacca tttattaaag atttaacgga tgatgattta
     1801 aaagaagcga tacaaagagc catcaatgta aaaaaaggtg aattctttat agcgcgtgca
     1861 atcacaccac aagataaaag attaaaaagt gacaaagcat ttattgcatt tttagaagaa
     1921 accttcgatc agttcttacc attttattct gcttaaataa ctttgtttaa ataatagagc
     1981 acgtaatctc atccatgatt tcgtgctttt tttcttacca ttaaatcgaa cgttcaacat
     2041 aataattcat acttttaaaa aaaataaaat aaatttaggt tgacctaaac attttattag
     2101 gttattatat tgtccataag aagtagaggt gagtcaaaat gaataataaa agacattcaa
     2161 caaatgaaca attaagttta gacgaaataa acaatacaat taaattcgat catcgcagtt
     2221 caaataaaca gaaattttta tcatttcttg gtcctgggtt attagtcgct gttggttaca
     2281 tggatcccgg aaactggata acatcaatgc aaggtggcgc gcaatatggc tatactttgc
     2341 tattcgtaat tcttatttca agtttatcag caatgttact tcaaagcatg acagtgagat
     2401 taggaatagc aacaggtatg gacttagctc aaatgacacg acattattta tcaagaccta
     2461 ttgctataat cttttggatt attgcagaac tagcaattat cgccacagat attgctgagg
     2521 ttattggtag tgctattgct ctcaatctcc tatttaacat acctttaatc gtcggtgcac
     2581 taataactgt acttgatgta tttttactac tatttataat gaaatatggt tttagaaaaa
     2641 ttgaagctat tgttggtaca ttaattttca cagtgttatt catctttata tttgaagtct
     2701 atatttcatc accacagttg aacgctgtgt taaatggatt tataccacat agtgaaatca
     2761 ttacaaataa cggcattctc tatattgcat taggtattat tggagctaca attatgcctc
     2821 ataatttgta cttacattca tcaattgtac aatctagaac atactcaaga cataacaatg
     2881 aagaaaaggc gcaagcgatt aaatttgcta cgatagattc gaacattcag ttatcaatcg
     2941 catttgtagt caattgctta ttattagtgt taggagcatc actatttttc aactcaaacg
     3001 ctgacgattt aggtggtttc tatgatttat atcatgcctt aaaaactgaa cctgtactgg
     3061 gtgcaacaat gggtgcaatc atgagtacat tatttgcagt tgcattatta gcgtcaggtc
     3121 aaaattcaac gattactggt actttagcag gacaaatcgt aatggaagga tttttaagat
     3181 tacacatacc aaattggtta agacgtttaa ttacacgttc ccttgctgtc attcctgtta
     3241 tcgtatgttt aatcattttt aaaggtaatg cagctaaaat tgagcagcta cttgtattct
     3301 ctcaagtatt cttaagtatt gctctgccgt tctgtttaat accattacaa ttagcaacta
     3361 gtaataaaga cttgatggga cctttctata ataaaacatg ggttaatatc atttcatgga
     3421 cacttattat tattctaagt attttaaatg tctatttaat cattcaaaca ttccaagaac
     3481 tacaaggtta aaaataaaac cattaaaatg ttggttgtat ctaaaaatta acaacttaac
     3541 attttaatgg tttttctttt gctatgaaca tattaatgat aaatatactc atcgtcctta
     3601 tgtgtagttt cttttgattc ttttttagaa atcaacataa tcatcacgat aatccataag
     3661 acaattgcaa gtatattaag agccatcaaa cttacaatta tcgcgataac aaaaagtata
     3721 atcgataatt ttctacgccc tttgatacaa attgtcgcaa atatagcata aagcgttgaa
     3781 attaataaaa taattgctaa tgctccaatt gttgtatgaa gcatatctaa agaaactttg
     3841 ccaccgttct gagataattg cttcgccaat tcttcacgtt gagatgcatt accacttttt
     3901 aaagaaaaat aggatagccc cataactaat aaataaataa tactaatact atttgcaatc
     3961 caagccatga ttaattcagg ttttctattc atcttaaaat cctttcttta tttaatattt
     4021 taataagtat actacgagcc tatagatata cgcacattaa tttacttgta tattaacata
     4081 tttttcatct aatttttata ataaacatct gaattcagat tgatggtaat ttaggtgtat
     4141 ggtaatgaga aatcgtcatt taaaattttg acacctaaac actttaatta aacgttatcg
     4201 ataattaacc tcatataaaa acccagatta ataagcaata gacctctact tactaatctg
     4261 ggtatcatca atatttattc ttgctctttt ttgtccttaa cttcacttga attgcgttct
     4321 tcagaaatat cattgttgta aatttgacgt cgctcttcaa aattcattcg acgttgacta
     4381 actgcgctag gttgattttt cctacgttct ttcattcgtt ggttataagc tttacgtttt
     4441 tcttttttct tagctttaat ttcggctttt tctctttttt gttgtgcttt caattctttt
     4501 ggatcaactt caggttctgc ataataacca tcatcttcaa tacctagttc ttcagtactt
     4561 ttttgtttgt atttagcctg tctggataat actgatggtt cttcctcagt gcgctcatca
     4621 tgattatatt cactttcaac tgcacgttta ggaaattgat catatttatc tctttcctgt
     4681 tcttgatcat atacagtgtt tgatttatcc aattcttttt catatttagg cacatcataa
     4741 taatcatctt tcggttgtgg gcgctcatac acaggttcac gataatcata aatcggtccg
     4801 ttatactctt cataatattg tggcgcaacc atttcaactt tatcttttct tataaatagc
     4861 atcatcgcaa taataaagaa taatattgga atgattaaag taacgataag taaaactaaa
     4921 ggaatcgtta caattgctga tattaagaat aagaaacctg acaagattct tatattcata
     4981 gatatgagag ctaaaaatga aatcaacaaa caaacaataa agtaaacgat aatagcccat
     5041 ataccatttt gtaaccatat tacaaattgt gttgtattat agccatttcc agctaataat
     5101 tgctggataa atgcattatt gtttaatgta ttttcaagat tagcaatcga tgtgttatta
     5161 ctgaatgaaa caagtgctat aaacatcgta atgacagtaa gcactaataa catcacccaa
     5221 cataaccaac ctagaacttt ttcagttaat ctacttactg gacgtttaat ttgagtaaat
     5281 tgttctccag tcattcgtta caactcctta tagtacttat cccgttatta taactaaata
     5341 tacagtaaat aactactatt tatgatttta ttttaatgac attttgaaat tcaaaaagtt
     5401 ttcattgtat tcacttaaaa cttcaggtcc taaatcttta taaacttcaa ggcgttcttg
     5461 ctctttctta gtcggataga aacgatggtc gtctttaatc tttttaggca acaattgtcg
     5521 agcagccttg tttggcgttg catagcctac gaattctgta ttttgcttgt tatttttagc
     5581 atctaataaa aaattcataa atttatatgc accctcttta ttttgtgccg tttttggaat
     5641 taccatattg tcgaaccata aattcgatcc ttctttagga ataacatagt tatatttatc
     5701 cccatcttgc actagaggtg ctgcaacacc actccaaaca accgctatgt taccttcatt
     5761 ttgttgaagc atcatggtaa tttcatcacc tacgacacct cttacttgtg gtgctagttt
     5821 ggttaaatct cgctctgctt cttttaaatg gtgcgaatta cggtcattaa gattataccc
     5881 aagtttattc aaactcatgc ctataatctc tctagcacca tcaactagta aaatttggtt
     5941 tttaaattta ggattataca acgacttcca actatcaaat gattcatttg gatacttttc
     6001 tttattatat aaaataccta cagttccaaa gaaataaggt aaagagtatt tattgccttt
     6061 atcaaatgac atattcatat aatctgaatc ca
//
LOCUS       DAHHRR010000134         6053 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.135, whole genome shotgun sequence.
ACCESSION   DAHHRR010000134 DAHHRR010000000
VERSION     DAHHRR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.135"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            55..1056
                     /gene="argF"
                     /locus_tag="NBI42_002298"
     CDS             55..1056
                     /gene="argF"
                     /locus_tag="NBI42_002298"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCD1778690.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNVALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAVNGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1079..2011
                     /gene="arcC"
                     /locus_tag="NBI42_002299"
     CDS             1079..2011
                     /gene="arcC"
                     /locus_tag="NBI42_002299"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD1778691.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPSFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKDKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            2182..3738
                     /locus_tag="NBI42_002300"
     CDS             2182..3738
                     /locus_tag="NBI42_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCD1778692.1"
                     /translation="MENTNNESEKEKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKKDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            4046..4273
                     /locus_tag="NBI42_002301"
     CDS             4046..4273
                     /locus_tag="NBI42_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778693.1"
                     /translation="MTHLTKVLDTLTGMCVVLLFSKYFVAYANMVFDWKLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRKRKNNGV"
     gene            complement(4589..5536)
                     /locus_tag="NBI42_002302"
     CDS             complement(4589..5536)
                     /locus_tag="NBI42_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCD1778694.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMLKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINNLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLENVYPSWTVLFIGIAIAGLTVPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     ILLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNCLPVLMFPVIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFFKKENQDKFLRNASQ"
     gene            5785..5972
                     /locus_tag="NBI42_002303"
                     /pseudo
     CDS             5785..5972
                     /locus_tag="NBI42_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2144 a          811 c         1150 g         1948 t
ORIGIN      
        1 attttgtatg cgcttacaat ttaggtgtaa ctaaaataaa aggagttgtt attaatgaaa
       61 aatttacgaa acagaagttt tttaacttta ttagactttt cacgacaaga ggtagaattc
      121 ttattaacac tctccgagga tttaaaacgt gctaaatata ttggcactga aaagcctatg
      181 ttaaaaaata aaaatgttgc actgttattt gaaaaagatt ctacaagaac tcgatgtgca
      241 tttgaagttg cagcgcatga tcaaggtgct aatgtaactt atttaggccc aactggatca
      301 caaatgggta aaaaagaaac aactaaagat actgcacgtg tgcttggtgg aatgtatgat
      361 ggcattgaat accgtggttt ttcacaaaga acagtagaaa ctttagctga atattcaggc
      421 gtaccagtgt ggaatggttt aactgatgaa gatcatccta ctcaagttct tgctgatttc
      481 ttaacagcaa aagaagtttt aaaaaaagat tatgcagata ttaactttac atatgttgga
      541 gatggacgta ataacgttgc aaatgcatta atgcaaggtg ctgccattat gggtatgaac
      601 ttccatttag tttgtccaaa agaattaaat ccaacagatg aattattaaa tcgctgtaaa
      661 aatattgccg ctgtaaatgg tggcaacata ttaatcacag atgatattga ccaaggtgta
      721 aagggttcgg atgtaattta cactgacgtt tgggtatcaa tgggtgaacc tgatgaagta
      781 tggaaagaac gacttgaatt attgaaacca tatcaagtaa ataaagaaat gatggataaa
      841 acaggtaatc caaatgttat ttttgagcat tgcttaccat ctttccacaa tgctgatacg
      901 aaaattggtc aacaaatttt tgaaaaatat ggaattcgag aaatggaagt tacagatgaa
      961 gtattcgaaa gtaaagcttc agttgtattc caagaagctg agaacagaat gcatacaatc
     1021 aaagcagtca tggttgctac attgggtgaa ttttaaatga tataaggaag tgaatatgat
     1081 ggcgaaaata gtagtagcat taggtgggaa tgctttagga aaatcacctc aagaacaact
     1141 cgagcttgtt aaaaatactg cgaaatcatt agtaggatta ataacaaaag gacatgagat
     1201 tgttattagt catggtaatg ggccacaggt tggaagcatt aatttgggac ttaactatgc
     1261 tgcagaacac aatcaaggtc cgtcatttcc atttgctgaa tgtggtgcaa tgagtcaagc
     1321 ttacatcggc tatcaattac aagaaagctt acaaaacgaa ttgcattcta ttggaatgga
     1381 taaacaagtg gtaacactag tgacacaagt tgaagttgat gaaaatgatc cggcatttaa
     1441 caatccttca aaaccaattg ggttatttta caacaaagaa gaagctgaac aaattcaaaa
     1501 agataaagga tttatatttg ttgaagatgc tggaagaggg tatagacgtg ttgttccttc
     1561 accacaaccc atctctatta ttgaattaga gagtattaaa acacttatta aaaatgatac
     1621 actcgttatt gctgctggtg gtggaggtat accagtaatt agagagcaac atgatggttt
     1681 taaaggtatt gatgcagtta tagacaaaga taaaacaagt gcgttgttgg gtgctaatat
     1741 tcaatgcgat caattgatta ttttaacagc aattgattat gtatatatta attttaacac
     1801 tgaaaaccaa cagcctttga aaacaacaaa tgttgatgaa ttaaaacgat atatagacga
     1861 aaatcaattt gcaaaaggaa gtatgttacc aaaaattgaa gcagccatat catttattga
     1921 aaacaatcca aaaggaagtg tgcttataac atcattaaat gaattagatg ctgccttaga
     1981 gggtaaagta ggtactgtga ttaaaaagta attgaattga aacgcttttc aattactata
     2041 tgtcaaatgc atgattttta ttatttatgt gcacccctga aaataatgcc tctattttga
     2101 tgcggggtgc actttcttaa tttatattta taaaatcttt aaggtagaaa tgctaggtta
     2161 aatgtcgaag gagatgaaac cgtggaaaat acaaataatg aaagtgaaaa ggaaaaacgc
     2221 tttaaattaa aaatgccagg tgcatttatg attttattca ttttaacggt tgttgcagtt
     2281 atagcaacat gggttattcc tgctggtgca tattctaaac tttcttacga accttcatcc
     2341 caagaactaa agatagttaa tcctcataac caagtgaaaa aggttccagg tacacaacag
     2401 gaactagaca aaatgggggt taaaattaag attgaacaat ttaaatcagg tgcaattaat
     2461 aagccggtat caattccgaa tacttatgaa agattaaagc aacatccagc tggaccagaa
     2521 caaataacaa gtagcatggt tgaaggtacg atagaagcgg tcgatatcat ggtattcatt
     2581 cttgtattag ggggacttat tggcgtagtt caagccagtg gttcttttga atcaggattg
     2641 ctagctttaa caaagaaaac aaaagggcat gaatttatgc taattgtgtt tgtatcaata
     2701 ctaatgatta tcggcgggac gttatgtggt attgaagaag aagctgtcgc attctatccg
     2761 attttagtcc ctatatttat agcgttagga tacgattcta tcgtttcagt tggtgccata
     2821 ttccttgcca gctctgtcgg tagtacattt tcaactatta acccgttctc ggttgtaatt
     2881 gcctctaatg ccgctggtac aacttttacg gatgggttgt attggagaat aggtgcttgt
     2941 attgtcggtg cgatttttgt tattagttat ttatattggt actgtaaaaa aattaaaaaa
     3001 gatcctaaag catcatattc ttatgaagac aaagatgctt ttgaacagca atggtctgta
     3061 ttaaaagatg acgatagtgc ccattttact ttgcgtaaga agataatcct tacattattt
     3121 gtactaccat ttccaattat ggtatgggga gttatgacgc aaggttggtg gttcccagtt
     3181 atggcttcag catttttaat atttacaatt ataataatgt ttattgctgg gacaggtaaa
     3241 tctggattgg gagaaaaagg aactgtagat gcatttgtca atggtgcatc aagtttagta
     3301 ggtgtatctt taattattgg cttagctcga ggtattaatt tagtgttgaa tgaaggtatg
     3361 atttcagata caatcttaca cttttcatca tctttagttc aacatatgag tggaccatta
     3421 tttatcattg tattactatt tattttcttt tgtttaggtt ttattgtgcc atcatcttct
     3481 ggattagcgg tattatcaat gcctatcttt gcaccactag ctgatacagt aggtatacca
     3541 agattcgtca tcgttacgac atatcaattt ggtcaatatg cgatgttatt cttagcgccg
     3601 actggacttg ttatggccac actacaaatg ttaaacatgc gatattcgca ttggttccga
     3661 tttgtatggc cggtagttgc ttttgtattg attttcggtg gcggagtact aattacgcaa
     3721 gtactaattt attcataatt tgaaatgcta tattataaaa aatactaatt gggttttatg
     3781 catctcgtag gtttgtagaa atactaaact aagcgaggtg cattattatt ttgattaaga
     3841 aaataatggc ggtaatgata acactagtaa gtaattgata caatgttcta tttaataatg
     3901 atatttttaa aatatgtttt taatgtaatg ttagatctat ggtatattat atttatcgtg
     3961 gtaaatgtgt atttgctgta aagagttttt gtaacacatg atacatatgg ttgtgaaaat
     4021 agcatatata aaaggatggc tataagtgac acatttgaca aaggttttag atacactaac
     4081 tggaatgtgc gtagtattat tatttagtaa atattttgtg gcgtatgcaa atatggtatt
     4141 tgattggaag ttaagatggt atttgctaga aaacatacca catttgccaa ttatattatt
     4201 tattctgatg tttattttcg gagtaccttc tgaaatgata aaagatagga aaaggaaaaa
     4261 taacggcgtt taatttatcg atatttagag gtgataaaac ttgtcaacta ttacaaaaac
     4321 taaaataaaa atctttatta attattttat tggtatatta gccttggcgt ttgctgctta
     4381 tatattattt aaataaagca tagagtgaaa cgtgtttatg agctagacgt tctagtatag
     4441 ttactacagc tttagtcaga aggtatcatt gttgttcata cataagatca tattaaatca
     4501 aagaggtatt gatatacact aaaaagaggc aagatcacct gcctcttttt tagttattaa
     4561 atatgcgtgt taattcttgg tattagggtt actgagacgc attacgaaga aatttatctt
     4621 gattttcctt tttaaaaaag aagtgaagat atcctataaa gactctaagt actattacag
     4681 tggctaataa cacttcgata aacaaaaggc ctttccaaat gactggaaac ataagtacag
     4741 gcaaacagtt ctttaaagca gttgcagaga ttactaaagg gaatgtgaaa gctgaaaata
     4801 cgggcgaaaa aggttctttt agtaatttag gcaattgtat aatgatataa aaataaaata
     4861 tttgagctaa tagtaaaagt ataattacga taaaagcatt cgccttagga aaggctataa
     4921 catatgctgc agcgactaaa gaaaatggtg cacaaattgt cgatgtgttc ggtttgattg
     4981 acgtctgcaa tggaaatgct tttaatcgct tgaaaactat aggtaagaca atacaagtag
     5041 ctacaaagcc atatattact gttaattgac ctatgaaaaa atatccgctg acgggtaccg
     5101 tcaatcctgc gatagcaata ccaataaaaa gtacagtcca cgaaggataa acattctcaa
     5161 gtgaaaaatc ttttaaatat ttaattgaaa aaataatcat atgtgtcata attcccacaa
     5221 ggcataaaat ccaaataggc gttattaaat tattgataaa tgttatatta ctaaaaaatg
     5281 tatttaaata agtagtacct aaaaagccag acatgaaaaa tgttgtgaac acagatgaaa
     5341 ctagagggct gtttaattgt tctttcacat tattaaaata tttgagcata gtacataaaa
     5401 ggtgaatcca aatcaagaaa gcaaagattc cgcaaacagc gtttaaagta agagataagt
     5461 cttttaatag attacccagg cctaataatc ctaagactag tcctgacgtt actagaggtg
     5521 ctttttgaag tctcatgatt tacaaccttt cttatgtgat tttcttcact aattatatca
     5581 tgatagctat ggcctattaa taagaagagt gtgtactatt acgtttttag agtatgtata
     5641 ttggattgat agacacataa ttgacattta aatctcaaaa ttaatgatat ttgaggtatg
     5701 ctttgcaaac taaatattgg aatatttgga aaagtattta tttaatttaa atgatgattg
     5761 aagatttaca agggggtgta caaattgaga aatcaaattc aaaaactatt agacagtgat
     5821 ttgagcagtt taatatatcg aaacaaacag gagtgccaca aagcacaata cacagaatga
     5881 gaaaaaatga aagatcatta gacaatatgt cattgaaaaa cgctgaacta ctttataaat
     5941 ttgccaatgg tatatttagt gatgaagatt aagaaaaata attagtgagt taaataatta
     6001 gaagggagtg ggacagaaat gatattttcg caaaatttat ttcgtcgtcc cac
//
LOCUS       DAHHRR010000135         5999 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.136, whole genome shotgun sequence.
ACCESSION   DAHHRR010000135 DAHHRR010000000
VERSION     DAHHRR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.136"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..984
                     /gene="emp"
                     /locus_tag="NBI42_002304"
     CDS             <1..984
                     /gene="emp"
                     /locus_tag="NBI42_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HCD1778695.1"
                     /translation="FATQFMNSNHAKASLTESVDKNFVVPESGINKIIPSYDEFKSAP
                     KVNVSNLTDNKNFVVSEGKLNKISDPSAASKIVDKNFVVPESKLGNIVPEYKEINNRV
                     NVATNNPASQQVDKHFVAKGPEVNRFITQNKVNYHFITTQTHYKKVITSYKSTHVHKH
                     VNHATDSINKHFIVKPSEAPRYTHPSQSLMINHYFAVPGYHAHKFVTPGHASIKINHF
                     CVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYDYKYYYSYKVVKGV
                     KKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGSLPAPRV"
     gene            1319..1840
                     /locus_tag="NBI42_002305"
     CDS             1319..1840
                     /locus_tag="NBI42_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778696.1"
                     /translation="MKRKVLVLTMGVICATQLWHPNHANALVTESIETNFVVKDSDNK
                     NILQTHTEITTEEKFSIVEKSQLNKLKSLSNDNYIEYDLHTNQTGIKKGWLYGYSEID
                     SSHFTDRDKRVIRRDHVKEAQSLINDYKYTQKYEDFAKATAKVSTLSQSHQNYLNKQI
                     DNVNNQIEKTEKR"
     gene            2181..2867
                     /locus_tag="NBI42_002306"
     CDS             2181..2867
                     /locus_tag="NBI42_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCD1778697.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCL
                     VLILVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKKKLNI
                     WSEDNADSGQ"
     gene            3224..3424
                     /locus_tag="NBI42_002307"
     CDS             3224..3424
                     /locus_tag="NBI42_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1778698.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(3918..4136)
                     /locus_tag="NBI42_002308"
     CDS             complement(3918..4136)
                     /locus_tag="NBI42_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778699.1"
                     /translation="MSEWHINNQSTKPFLIQQAEEQLSIVKPLPNGSFQILTEIDLEN
                     GNVNNIDHSLLVSVDLKALEISIFDAVN"
     gene            complement(4198..4482)
                     /locus_tag="NBI42_002309"
     CDS             complement(4198..4482)
                     /locus_tag="NBI42_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778700.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNNGETETVANFFGNAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            complement(4569..5138)
                     /locus_tag="NBI42_002310"
     CDS             complement(4569..5138)
                     /locus_tag="NBI42_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778701.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIITKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAADWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKTY"
     gene            complement(5326..5514)
                     /locus_tag="NBI42_002311"
     CDS             complement(5326..5514)
                     /locus_tag="NBI42_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778702.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            complement(5543..5803)
                     /locus_tag="NBI42_002312"
     CDS             complement(5543..5803)
                     /locus_tag="NBI42_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778703.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFTG"
BASE COUNT         2300 a          827 c          901 g         1971 t
ORIGIN      
        1 tttgctacac aatttatgaa ttcaaatcac gctaaagcat cattgacaga gagtgttgac
       61 aaaaactttg tcgtaccaga atcaggtatt aataaaatca ttccatctta cgatgaattt
      121 aagagtgcac caaaagtaaa tgttagtaat ttaactgaca ataaaaattt tgtagtttct
      181 gaaggcaaat tgaataagat ttcggatcct tcagcagcaa gtaaaattgt agataaaaac
      241 tttgtcgtac cagaatcaaa gttaggaaac attgtgccag agtacaaaga aatcaataat
      301 cgcgtgaatg tagcaacaaa caatccagct tcacaacaag ttgataagca ttttgttgct
      361 aaaggcccag aagtaaatag atttattacg caaaacaaag taaactacca cttcattact
      421 acgcaaaccc actacaagaa agttattact tcatacaaat caacacatgt acataaacat
      481 gtaaatcatg caacggattc tattaataaa cactttattg ttaaaccatc agaagcacct
      541 agatatacac acccatctca atctctaatg ataaatcatt attttgcagt tcctggatat
      601 cacgcgcata aatttgttac accagggcat gctagcatta aaattaatca cttttgtgtt
      661 gtgccacaaa taaatagttt caaggtaatt ccaccatatg gtcacaattc acatcgtatg
      721 catgtaccaa gtttccaaaa taatacaaca gcaacacatc aaaatgctaa agtaaataaa
      781 gcatatgact ataaatacta ctattcttat aaagtagtta aaggtgtgaa gaaatatttc
      841 tcattttcac aatcaaatgg ttataaaatt ggtaaaccat cattaaatat caaaaatgta
      901 aattatcaat atgctgttcc aagttatagc cctacacact acgttcctga atttaagggt
      961 agcttaccag caccacgagt ataaaaattg gcactaagtt tacgagatat gataaatacc
     1021 tattatttta aatatagtct acaatctatg tggtcgtagg ctgtattttt tatgctaagt
     1081 gatttatatc tatcaacaaa tcacttcaca tttctacttt gaaagttgaa tagtaacata
     1141 atcttaaata attattttat taattaatat aaatataaga cttgaaataa tagttaatag
     1201 tttgtttatc gcaagttaat tattgtttct aaaatattgg tatataattt tcaatggcga
     1261 agaaaacagg gtaaaaaagt cggtttttaa atcaaagcaa ataagggagc ataaacaaat
     1321 gaaaaggaaa gtactagtac taacaatggg tgtaatttgt gcaactcaat tatggcatcc
     1381 taatcacgca aatgcattag tgacagagag tattgaaact aattttgttg taaaagattc
     1441 ggataataaa aatattttac aaacccatac tgaaattact actgaggaga aattttcaat
     1501 agtagaaaag agtcaattaa ataaattaaa atcactatct aatgacaact acatagaata
     1561 tgatttacat actaatcaaa caggcattaa aaaaggttgg ttatatggtt atagtgaaat
     1621 tgactcatca catttcacag accgtgacaa acgcgttatt agacgtgatc atgttaaaga
     1681 agcacaaagc ttaattaatg attataaata tacgcaaaaa tatgaagact ttgctaaagc
     1741 tactgcaaag gtaagtacac ttagtcagtc tcatcaaaat tacttaaata agcaaatcga
     1801 taatgtgaat aatcagattg agaaaactga aaaacgctaa tccaaagtaa attataagtt
     1861 atacatcttg tttttaaatg acaatttatc cccgtaaata taataaataa tcttttcaaa
     1921 ttccgcatag atatagagac gctaataaac ctctttgtct cgatatgata gtctgcaacg
     1981 attcatgttg taggcttttt aattttacaa ataaggctaa atatataagt tctggcacct
     2041 aaaatataga aaatacataa aagtaagtat agttatttta ttataattat taaattttta
     2101 ttaattaatt gtaaaaatgt agaattataa ttaattaacg tttaatatta aaattaacta
     2161 aaaagaaaga ggtgttagtt atgacagaat acttattaag tgctggcata tgtatggcaa
     2221 ttgtttcaat attacttata gggatggcta tcagtaatgt ttcgaaaggg caatacgcaa
     2281 agaggttttt ctttttcgct actagttgct tagtgttaat tttagttgta gtttcaagtc
     2341 taagtagctc agcaaatgca tcacaaacag ataacggcgt aaatagaagt ggttctgaag
     2401 atccaacagt atatagtgca acttcaacta aaaaattaca taaagaacct gcgacattaa
     2461 ttaaagcgat tgatggtgat acggttaaat taatgtacaa aggtcaacca atgacattca
     2521 gactattatt ggttgataca cctgaaacaa agcatcctaa aaaaggtgta gagaaatatg
     2581 gtcctgaagc aagtgcattt acgaaaaaaa tggtagaaaa tgcaaagaaa attgaagtcg
     2641 agtttgacaa aggtcaaaga actgataaat atggacgtgg cttagcgtat atttatgctg
     2701 atggaaaaat ggtaaacgaa gctttagttc gtcaaggctt ggctaaagtt gcttatgttt
     2761 acaaacctaa caatacacat gaacaacttt taagaaaaag tgaagcacaa gcgaaaaaaa
     2821 agaaattaaa tatttggagc gaagacaacg ctgattcagg tcaataatgc tcattgtaaa
     2881 agtgtcactg ctgctagtgg cacttttata atttttagat cacgatatga tttattatcc
     2941 attcagaatt aaaaaagtaa ttagtatcaa aagcaagtgt atttaatatt agaaaataaa
     3001 aattttaaat ttagtattaa aatggaatat tactatatat ttcaatgtgt attatcacag
     3061 aaaataaaat aatgctttac ttctatattt aaaagtgtat aatgaaagtt aagtaataaa
     3121 gagcgtgaag aaaaatgtga gttatttata tagaatattc tccttttcat ttatgaattt
     3181 gttacaaaat atttagtgca aaagcacgac ggaggtattc aatatgaata acggtacagt
     3241 taaatggttt aatgcagaaa aaggttttgg tttcatcgaa agagaagatg gtagcgacgt
     3301 attcgtacac ttctcagcaa tcgctgaaga tggatacaaa tcattagaag aaggccaaaa
     3361 agttgaattc gacatcgttg aaggcgaccg tggcgagcaa gctgcaaacg tagttaaaat
     3421 gtaattttaa ctttttcaaa cagtccttat tatagggctg tttttttatg ctttaaatcg
     3481 atagcagttg gtgtggtaaa agcactagct gttatttttg tccaataaat ttaggtggag
     3541 atctaacaat atataatggt tctaaaataa atcgaactga tggaaaagtt ttttactttt
     3601 catctgtccg acttttgatt ttgaataaaa aaaagcgcca atacagaact ttaataatga
     3661 cgagaattaa agtctgtata tggcgataac aagaagtaat gttaaacact caaaatgttt
     3721 aacaataata ggataccaca tcgcataata tcttactact taattaacaa tttaactaat
     3781 caactttttg ttaatttttt attaagactg attaattatt gagaatattt attgttttta
     3841 aaatctcata ataattcagt aatcttgttt tcatttaaaa ggtggaacat taaaataatt
     3901 aaataaaaat attgcgttta atttacagcg tcaaatatac ttatttctaa tgctttgagg
     3961 tctacagaaa caagtagaga atgatcgatg ttattaacat tgccattctc caaatctatt
     4021 tctgtgagta tttgaaagct accattaggc aatggtttaa caatagacaa ttgctcttcc
     4081 gcttgttgta ttaaaaaagg ttttgtagat tgattattaa tatgccattc actcatgtat
     4141 gtttttcact cctgctttaa tatagggtta aaaagtttat agttgagaca ttcatgttca
     4201 accaaaattt tgttcaaatt caataaatgt cttgtttaaa atagaaatat tgtaaatgtt
     4261 atcgtccaaa acttcaccag ttaagtattt gttttgaatt aaaatttggc agttagttaa
     4321 gaagtcttga taatcacgat cgcaaaaata gttttcacgt gcatctttag cattgccaaa
     4381 aaagttagcg actgtttctg tttctccatt attcgaacgt tcaatatata atttgtaaaa
     4441 tttagctatt gtatactttt gttctttagt tagttcattc aaaatattgg acctcctgaa
     4501 atatcatttg taatctatac ccaatttatt gcaaaacaca aactaattta accatttgat
     4561 gaaacagttt aataagtttt aacaagcctt agtttatatg gatctataaa attatcttta
     4621 attgcatagg gtgaaataat atgtagtcca taacttttaa cagatttttc acttacacca
     4681 aatttataag cttgatagat aattttagta caatacgtaa attttttgct gttcaaattt
     4741 aatgtaacta gataacggtg atttgtattt tcatagtttt tcttaaccca gtcagccgct
     4801 tttttacctg caccaggata gctgcaacga taaactttca tccaatcatt tttgccactt
     4861 gcataattat atttaaaaga ttcgaaggat tgtgtagttg gtttgtcgcc aggcccctca
     4921 atttgcaaaa tcgttttatc atcaatcgcg atactacaat gaccaaaaaa tccccacatg
     4981 acagggcctt ttgtaataat aatatcacca ggttgtaatt ggaatttgtc atcttgaatt
     5041 tctgaatact tattatctgc aattgttttt ggtgagttta taggggatac gacaacgaat
     5101 aatataagta aaattatcgt tcgtttaata tagttcactt aaaagctcct tgttgaagaa
     5161 atatatgtaa atagtcttaa attagaattg taatctttaa taagcttgta agactaaaac
     5221 atagcttaaa tattaaagta tgagagtgtg aaatgtctat taagaataaa aaacagtcta
     5281 aaacatcttt gagacgtttc agactggata taaattgaat ttcatttata gcacaccaaa
     5341 tataaatgtg tatattaata tagcgaatcc gaatatatag agaatgacag tgaaacttaa
     5401 ataggactct tttttagatt ctttgccagt ttttttcatt aatacgagta taaacgtagc
     5461 ggcaactaag aaaattaatg ctagccaaaa aagaattgca aaatgtaaag acatgtgaaa
     5521 ccctccttat ataccgtaat tattaacctg taaaactata aaaaatagga taaatgtaat
     5581 agagaacgag tacgatattt ataaaaaata atatttcact taaccagttt ttagttatca
     5641 ttgcaatggt aaaggatacg atgagtatca caccacaaat gataatacca ggcaggagcc
     5701 aacataaatc atctaaatct ttattatatg tgattaaaat attaaagata acaaaagtgg
     5761 tagtaataac tatattgata gcatttaaca aaatgttatt catgactgac acctactagt
     5821 ataaaaatag cttcttaata aatatgataa cactattttc caataggtaa ataaaaagta
     5881 tagttatttt taaaatatta tttgtagaat gctaaagttt attaattcaa acaagtgagc
     5941 atatatagag gtgtatagta ttaatatgaa aagaatattt acatactaaa aacccttaa
//
LOCUS       DAHHRR010000136         5989 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.137, whole genome shotgun sequence.
ACCESSION   DAHHRR010000136 DAHHRR010000000
VERSION     DAHHRR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.137"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            155..871
                     /locus_tag="NBI42_002313"
     CDS             155..871
                     /locus_tag="NBI42_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1778704.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            871..1344
                     /locus_tag="NBI42_002314"
     CDS             871..1344
                     /locus_tag="NBI42_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCD1778705.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTYSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1436..1552
                     /locus_tag="NBI42_002315"
     CDS             1436..1552
                     /locus_tag="NBI42_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778706.1"
                     /translation="MLQLFLMKLTLNDFENDKTILFIILKNHCYEEVCVLSN"
     gene            1697..2341
                     /locus_tag="NBI42_002316"
     CDS             1697..2341
                     /locus_tag="NBI42_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCD1778707.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNFEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2457..3323
                     /locus_tag="NBI42_002317"
     CDS             2457..3323
                     /locus_tag="NBI42_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1778708.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            3803..5023
                     /locus_tag="NBI42_002318"
     CDS             3803..5023
                     /locus_tag="NBI42_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCD1778709.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKQPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAVFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMVLIFF
                     TKNQKITKEDVIST"
     gene            5020..5508
                     /locus_tag="NBI42_002319"
     CDS             5020..5508
                     /locus_tag="NBI42_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HCD1778710.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPVIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIARAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(5607..>5989)
                     /locus_tag="NBI42_002320"
     CDS             complement(5607..>5989)
                     /locus_tag="NBI42_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778711.1"
                     /translation="IGIVSFLKGFLGFFMEKSGSNLYFVSFPFSVVVGFFIVFLFIWF
                     SFKTIQLQCFNNSNWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIF
                     SFIVMPIGLRIERNISKKHSHTFL"
BASE COUNT         2123 a          800 c         1095 g         1971 t
ORIGIN      
        1 tcggcacatt tttaatttat acatatttta aaactaagta acagattgaa gaaatcgtag
       61 ttcaataatg ttaattatga aaatgtatat aatcataaaa aaatcatgta taatatatgt
      121 tgttaattaa acagttcgaa agcgagatga cattatggga cgtaaatgga ataacattaa
      181 agaaaaaaag gcccaaaaag ataaaaacac aagtagaata tatgcgaaat ttggtaagga
      241 gatttatgtt gcagcaaaat ctggtgaacc caatccagaa tctaaccaag ctttaaggtt
      301 ggtgcttgaa cgcgctaaga catattcagt gccgaatcat attattgaaa aagcaataga
      361 taaagctaag ggtgctggag acgaaaactt tgatcaccta agatatgaag gatttggacc
      421 tagcggatca atgctaattg ttgatgcgtt aacaaataat gtaaatcgta ctgcctctga
      481 tgtgcgagct gcttttggta aaaacggcgg taatatgggt gtatctggat cagttgctta
      541 tatgtttgat catgtggcaa catttggtat tgaaggaaag tctgttgacg aaatacttga
      601 aacattaatg gaacaagatg tagatgtaaa tgatgtgatt gacgataatg gattgacaat
      661 agtctatgct gaaccagatc aatttgcagt cgttcaagat gcgcttcgtg cagcaggtgt
      721 tgaagaattt aaagttgctg aatttgaaat gttacctcaa acagatattg aactttctga
      781 agcggaccaa gtaacatttg aaaaattaat cgatgcatta gaagatttag aagatgtaca
      841 aaacgtattc cataatgtgg atttgaaata atgaaatcag cagaacaatg gattgatgaa
      901 ttgcaacttg aatcacatcc tgaaggtggt ttctatagag agacaatccg agaagtattg
      961 aaagatggac gcagagcgcc gtttagtagt atttattttt tacttacaga tgacaatatt
     1021 tcgcattttc atcgaattga tgctgatgaa gtatggtact atcatgctgg tgattcttta
     1081 acaattcata tgataaatcc ggatggggaa tatacgactg caacattggg tactgatatc
     1141 caaaatggag atgtattgca atatgtagtg cctaaaggaa caatttttgc ttcttcaatc
     1201 gaaatttcaa atacttatag tttagtaggt tgtatgtgtc aaccggcatt tgagtttaag
     1261 cagtttgaat tgtttaagca atctgaatta attacacaat atccgcatct taaatcagtg
     1321 attgaaaaat atgctttaaa ataaaaatga tcaatgaagt ggtttgaagg ttgttaataa
     1381 acctttgagt cacttcattt ttgtatgtat tcttgattga atcagaatag aattgatgct
     1441 acagcttttt ctaatgaaac taacattaaa tgattttgaa aacgataaga ccatattatt
     1501 tataattttg aaaaatcatt gttatgaaga agtatgtgta ctatctaatt aatattgtct
     1561 attttaagta aattgaagcg agttgtagta aaataataat aaatattttc atatgattaa
     1621 caaaaactat aaaactgtat catgacatga catcattcat gtagttttat ataataatga
     1681 caaggagttg gtgactgtga aagtaaagta tatagataaa cgtcactggc gtcgcctaat
     1741 tgatagggaa tacacagagg taaaagttaa taataatagg ttcaagggta ttataggctt
     1801 agtcacgatg aaaaaggttc gtgatccttt agaggtgacg gtagttggac aaaatattat
     1861 tgtcgcagat gacaattata aatggttgca aatactacct gaaaagaaac gttatagtat
     1921 aactgtaatg tttgataata aaggcaatcc attagaatat tattttgata taaatatcaa
     1981 aaatataacg caaaaaggta atgcacgtac agtagattta tgtttagatg ttttagcatt
     2041 accaagtggt gaatatgagt tagtagatga agatgattta atgtttgcat tagaaagtga
     2101 gcaaattaca aaaaagcaat ttcatgaagc atatatgatt gcgcatcaaa ttatggcaga
     2161 gttagaaaat gattttaaag gattccaaaa gaaaatcatg tactgcttta ataaaattaa
     2221 tgcaaaggct caaaaaaatc atcaaaagcc acaaaataaa actaattttg aaaaaagcaa
     2281 acaaataaaa cctaagcaat ataatcaaac taaaaatcac caacaacaaa agaaaaacta
     2341 agtaattcaa gctgcagcca taccaataaa attggtgaaa ttattcgtta gtaatgaact
     2401 catgaatgct cgtagtcatg aaagttcaat aattgaataa tttatggggg gaaattatga
     2461 agattgaaga ctatcgttta ctaataacat tagacgaaac gaaaacgtta cgtaaagcgg
     2521 ctgaaatttt atatatatct caacctgctg ttacacaaag actaaaagct attgaaaatg
     2581 cttttggagt agatattttt atcagaacaa aaaaacaatt gattacaaca actgaaggaa
     2641 caatgattat tgagcatgcc cgtgacatgt tgaaaagaga acgattattt tttgataaaa
     2701 tgcaggcaca tattggtgaa gtgaatggaa cgatatcaat agggtgttct tcattgattg
     2761 gacaaacctt acttcctgaa gttttgagcc tatataatgc ccaatttcct aatgttgaaa
     2821 ttcaagtaca agttggttca actgaacaaa ttaaagcaaa ccatagagac tatcatgtta
     2881 tgataactcg tggaaataag gtaatgaatt tagcaaacac acatttattt aatgatgatc
     2941 attattttat ttttccaaaa aatagacgag acgatgttac aaagttgcca ttcatagagt
     3001 ttcaagctga cccgatttat ataaatcaaa taaagcaatg gtataacgat aatttagagc
     3061 aagattatca tgcaactatt acagtggatc aagtggcaac ttgtaaagaa atgttgatta
     3121 gtggtgtagg tgttacaatc ttgccagaaa ttatgatgaa aaatatcagc aaagaacaat
     3181 ttgagtttga aaaagtagaa attgataatg aaccgctgat tcgttcaacg tttatgagtt
     3241 atgatccgag tatgttgcaa ttgccacaag ttgattcttt tgtaaatctt atggcgagct
     3301 ttgttgaaca accaaaggcg tagtcttaga ctaatttaag gtgttagtat ttaattttaa
     3361 actattcggt taaattgaac gcagttggtt gctaatgcat caacagcaaa agagccccta
     3421 attaataaat taaaaggggg actaaggatt acagttggtt gctaatgcat caactgcata
     3481 agagccccta attaataaat taaaagggga taaaggatta cagttggttg ctaatgcatc
     3541 gactgcataa gagcccctaa ttaataaatt aaaaggggat aaaggaatac agttggttgc
     3601 taacgcaccg actgcataag agcccctaat taataaatta aaagtggacg aaggattaca
     3661 gttggttgct aatgcatcaa ctgcaaaaga gcccctaatt aataaattaa aaggggataa
     3721 aggaatacag ttggttgcta acgcaccgac tgcataagag cccctaatta ataaattaaa
     3781 aggggctcta agaagcgggg ttatggatat gtttgcagcg ttattacaaa taaagaatta
     3841 taaactcttt gttgctaata tgtttctact aggtatgggt attgcggtta cggtcccata
     3901 tcttgttctt tttgcaacta aagatttagg tatgacaaca aaccagtatg gattacttct
     3961 tgcatctgca gcgattagcc agtttacagt aaattcaatt attgctagat tttcggatac
     4021 gcatcacttt aatagaaaaa ttattattat tctcgcatta ttaatgggtg cgcttggttt
     4081 ttcaatatac ttttttgtag atacaatctg gttattcata ttactatatg cgattttcca
     4141 aggattattt gcaccagcaa tgccccaact ttacgcatct gctagagaat ctatcaatgt
     4201 ttcaagctct aaagatagag ctcaatttgc caacacagta ttacgttcaa tgttctcatt
     4261 gggcttttta tttggtccat ttattggtgc gcaattaatc ggattaaaag gctatgctgg
     4321 attgtttggt ggaacaataa gtatcatttt atttacttta gtacttcaag tgtttttcta
     4381 taaagattta aacattaaac aacctattag tacgcaacaa catgttgaaa aaattgctcc
     4441 taatatgttt aaagacaaaa cgcttttatt accatttatt gcatttattt tattacacat
     4501 tggacaatgg atgtatacga tgaatatgcc tttatttgtt actgattatt taaaagaaaa
     4561 tgagcaacat gtcggttatt tagctagttt atgtgctggt ttagaagtgc catttatgat
     4621 cattcttggc gttttatcat ctagattaca gactcgaaca ttgttgattt atggagcggt
     4681 ttttggtggt ttattctact tcagcattgg ggtatttaaa aacttctata tgatgttagc
     4741 aggacaggtg tttttagcta ttttcttagc ggttctttta ggaattggta ttagttattt
     4801 ccaagatatc ttaccagatt tcccaggcta cgcctcaaca ctattttcta atgcaatggt
     4861 tattggacag ttaggcggta acttattagg tggtgctatg agtcactggg taggtttaga
     4921 aaatgtattt tttgtatcag cagcatcaat catgttaggt atggtactta tattctttac
     4981 taaaaatcaa aaaattacaa aagaggatgt gatatcaaca tgacaattat tttatggcta
     5041 cttatcattg ctgccttcat gttagcattt gttgggttga ttaagccggt tattccttct
     5101 gttttagtgt tatgggttgg ctttctaatc tatcaatttg gctttcataa tcagcattta
     5161 tcatgggtgt tttatgtatc tatggcattg ctaacgatat taattttatg tgcggacttt
     5221 ttagctaata aatattttgt gaatcgcttc ggtggttcta agtttggaga gtatgcagct
     5281 ttaattggtg tcgttattgg atgttttgtt ttaccgccat ttggaattat tattatacct
     5341 tttattttgg tattcatagt tgaattaata caaggctatt catttgaaag agcagttaaa
     5401 gtaagtatag gttcaatcgt agcattttta acaagtagta tagctcgagc aatcattatg
     5461 tttataatga ttgtatggtt ctttatagat gctttattga ttaattaata aaaagcttat
     5521 tgcaatatat gtttttcggt aactgtaatt tagtgatttt atcattaaca gtaccaaatt
     5581 agcatatgat gtaataagct ttttgtttat aaaaatgtat gagaatgttt tttcgaaata
     5641 tttctttcaa tgcgtaagcc aataggcata actataaatg aaaatataat aaatacccaa
     5701 ttgcttacct gaatatatgg tccaaaggca agtaatgatt gtggaataaa gaatacatag
     5761 aaaaatccta caattagtag aatgattgcc aaataggtga atatccatat ccaatttgaa
     5821 ttgttaaaac actgcagttg tattgtttta aaactaaacc atataaataa aaagacaata
     5881 aagaatccaa cgactactga aaacgggaat gaaacaaaat ataaattact tccacttttt
     5941 tccatgaaaa atcctaaaaa tcctttgaga aaactaacaa tcccaatta
//
LOCUS       DAHHRR010000137         5963 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.138, whole genome shotgun sequence.
ACCESSION   DAHHRR010000137 DAHHRR010000000
VERSION     DAHHRR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.138"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(158..391)
                     /gene="nrdH"
                     /locus_tag="NBI42_002321"
     CDS             complement(158..391)
                     /gene="nrdH"
                     /locus_tag="NBI42_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCD1778712.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHQIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            589..1950
                     /locus_tag="NBI42_002322"
     CDS             589..1950
                     /locus_tag="NBI42_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCD1778713.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            1947..2966
                     /locus_tag="NBI42_002323"
     CDS             1947..2966
                     /locus_tag="NBI42_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCD1778714.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDESAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFAISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            3099..3761
                     /locus_tag="NBI42_002324"
     CDS             3099..3761
                     /locus_tag="NBI42_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCD1778715.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(3875..5572)
                     /locus_tag="NBI42_002325"
     CDS             complement(3875..5572)
                     /locus_tag="NBI42_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCD1778716.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(5572..5790)
                     /locus_tag="NBI42_002326"
     CDS             complement(5572..5790)
                     /locus_tag="NBI42_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCD1778717.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(5819..5941)
                     /locus_tag="NBI42_002327"
     CDS             complement(5819..5941)
                     /locus_tag="NBI42_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778718.1"
                     /translation="MNDMMNLVINQIVIKLSYRDDWNEFIDIYSNTILLGLTIN"
BASE COUNT         1900 a          873 c         1035 g         2155 t
ORIGIN      
        1 tttatgattt atgttaattt caaggaatgt tgttgctaac taattaaaca actaggtgtc
       61 attggcaaaa tgttgttctt caattaatgt taattaattt aaaattcgat atttaattta
      121 tgatagctaa aaatgatgaa gttgatttag atgaagatta atcttggata tttaatactt
      181 tgttgatttc atcaagatca acatggtaca ttggattgcc attcaacaaa ataaacggag
      241 ttgaaaaagc atcaaaatct atcatttcgt ttcgatattg ttgattgttg atatttcttt
      301 cttcaaaatc aatttgatgc tcatttagat aattttttac aaatgtacaa ggtggacaat
      361 cattctgcgt ataaacgatt atttctgaca tgtatcatgc tccttcgtgc tttattctaa
      421 tgtaatatac acaattatac attgaaattc aaatttgtga acacattgtg aactgacata
      481 aattatacac aatttcatag cgcataatgt tttaaaaata cacattatag caattataat
      541 taagttcatt gtgttgtcat caaaaaaaga aaaaggtgat gtttttaaat ggatacagtt
      601 gaaatcagtc ggtttttgac agctatgact ttagcagttc atatcatttt tgcaacgatt
      661 ggtgttggta tgcctttaat gttcgcaatt gcagaatttt taggtattcg caaaaatgat
      721 cttcaatata tagctatggc caaaagatgg gctaaagctt atacaattac tgtagcagtg
      781 ggagttgtta caggtacaat tataggactt caattatcat tgatttggcc tacatttatg
      841 gaaatgggag gacacgttat tgcacttcca ttatttatgg aaacatttgc gttcttcttt
      901 gaagctattt tcttaagtat atatttatat acttgggacc gttttaaaaa taaatggaca
      961 catttcttaa ttagtatacc agtaattatt ggtggctctt tctcagcatt cttcattact
     1021 tcagtgaatt catttatgaa tacgcctgca ggttttgagt tgaagaatgg gaagatggtc
     1081 aatgttcaac ctatagaagc gatgtttaac ccatcgttta tagttcgatc atttcacgta
     1141 attactacag ccggtatgac gatggcattt gttattgcat caatagcagc ttttaaatta
     1201 ttgcgtaatc gtcaacctaa agatactgtc taccataaga aagctttgaa aatgtctatg
     1261 atagttggat tcttttcaac tttactttct atgttggcag gggatttatc tgcaaaattt
     1321 ttgcataaat tccaacctga aaaattagca gcttatgaat ggcatttcga tacatcttcc
     1381 catgccaaat tattattatt tggtgtgtta gatgaaaaga ctcagcaagt taaaggtgcg
     1441 attgaattac ctggactatt gagtttctta gcagataata gtgtcaaaac taaagtgcaa
     1501 gggttaaatg attttccgaa aagtttacat ccgcctatga ttgtccatta tttctttgat
     1561 ttaatggtaa cgatgggaat tttatgtttt gtcatttcag gtgtttatgt cttaacttta
     1621 atgtttaaaa agcttagaaa gttttctact cataaatgga tgctttacgg aatattatta
     1681 acaggaccag cttcaatgct agctatagaa tttggatggt tcttaacaga gatgggtaga
     1741 cagccttgga ttgttcgtgg ttatatgcgc gtggcagaag cagcaacaca agcaggcgga
     1801 ataaccttcg ttacaatttt atttggcata ttgtacatca ttttaatgta tacatgtgca
     1861 tacgtattaa ttcgtatgtt taaaaataaa ccggcgtatg aagatgtaaa tcgtttagcc
     1921 aagaagcaag gaggagaaat agaaaaatga tttatgcatt tataggtata acagtgttat
     1981 ggttgttttt attttgctat atcattattg cttctataga ttttggggca ggtttcttcg
     2041 cattgcattc aaagttaact ggtgatgaaa agaaaattaa tcacttaatt tcacgttatt
     2101 taaacccagt ttgggaagtt acgaatgtat tctttgtttt cttcttcgta ggattcgtag
     2161 gtttctttcc agagtcaatc aaatatttag gcacggtatt gttaatacca ggttcaatag
     2221 cattgattat gatatcgttg agaaacagtt tttatgcatt tgaaaattat ggtcaagata
     2281 caaaattagc atggatgatc atgtatgggg taagtggttt attaattcca gcttcattat
     2341 caactgcttt aactattaca gaaggtggtt atattaatgt tcgaaacaat gttatcgatc
     2401 tagattgggt gcagttacta ttaagcccat ttgcttggtc tgtagtattc ttggcaatta
     2461 tttcagtttt atatatttca tcaggatttt tgacatatta tgctaaaaaa gcaaatgacg
     2521 aatctgcata taatttaaca agacaatggc acatattttt agggccaccg atgattatca
     2581 tttgtttatt cgtatttcta tcattacgca ttcaaaattc tgaacatttt tattcagctg
     2641 tttttgacta ctggtggatg tttgctataa gtttcttatt ctttgcatta gcttcattgt
     2701 taacattctt taagaaaaaa catggtttgg cttttgtatt tgttatttta caaatgatgt
     2761 tcgcgttctt tggctatgga attagtaaat tgccatacct tttatatccg tttgtaaaaa
     2821 ttacagatgc atatgttaat ccagaaatgg gctggacatt agtgattgtc tttattttag
     2881 gtttactttt attacttcca tcgttaatat tattattaag attatttgtt ttcgacaaag
     2941 aatatgttga aggaaagaaa tcataagttg agacgatacc ccaactttca tttacttgca
     3001 ttactgcatt tagataattt agttggggta ctcatttata ataattttaa atttatattt
     3061 tcaatgaatt gatgaaaatc atttaatgga gggcgagtat gggtaaagaa tatgtagtca
     3121 tcggtctagg tcgttttgga ggtagtatcg ttcgtgagtt gaatgcatta gacatggatg
     3181 taatggccat cgaccatgat gaaaatagag tgaatgaata tagtgatatc gctactcatg
     3241 cggttgttgc agacacaaca gatgaagcag ttatgaaaag tttaggtatc cgtaactttg
     3301 atcatgtcat tgtggcaatt ggtgaaaata ttcaatcaag tacgttgacg actttgattt
     3361 taaaagagtt aggtgtaaaa aaagtaactg ctaaagcaca aaatgattat catgcaaaga
     3421 ttttaaataa aattggagca gatacagttg tgcaccctga gcgtgatatg ggtagacgta
     3481 ttgcgcataa tgttgcgagt gcaagtgtac ttgattatct tgagttggca gacgagcatt
     3541 ctattgtaga attgaaagca actgaaaaga tggcggggca gtctatcatt gatttagata
     3601 taagagcaca atatggaatt aacattattg caattaaacg aggcaaagag ttcatcattt
     3661 caccaaatcc aaatattaat ttagaaatag gtgatatttt aattatgatt ggacatgata
     3721 atgatttaaa tcgctttgaa aaaaatattg cgacgagata atcgataatc atttgtttga
     3781 tatattcaaa taaatatcat atataaagaa aaagctacta acaatgtgtg gtgagtttaa
     3841 ttaaaacttc acttcaaagt tagtagcttt ttgattattt attgtttgat tctttttgtt
     3901 catttacctt cataatgact ggtaaaatca ttggttttct agctgttttt tcaaataaat
     3961 aaggttgtaa tgtttcaatg atagaagatt taatctgatg ccattgaata tctttatttt
     4021 gatttaactt actaataaca tcagttttga ttttgcgttg tgcatcataa attaattgac
     4081 ctgattctct catatataca aatcctcgag aaataatgtc tggaccagaa agtaatttat
     4141 ttgtattaaa atcaatacta acaacaacga taactaaacc ttcttcagat aatagcttac
     4201 ggtctcttat tacaacatta ccgatatcac cgataccact accatcaaca agtacattac
     4261 cagatggaat gcgaccagct ttacgtgctg aatcgtgtgt taaagctaag acatctccga
     4321 tatcaaagat gaagacatta tcttcttcaa cgccgcattc aacaccagtc tcaccgtgtg
     4381 cttttaacat acggtattca ccatgaatag gtaagaaata ttttggcttg attaatcgaa
     4441 gcattaattg ttgatcacct tgagaaccat gccctgaagt atggatgtta gaaatcttgc
     4501 tatggataac atctgcacca gctttataca aggaattaat agttctgtta atactttttg
     4561 tattacctgg gataggtgat gaactaaata caacggtatc ttcaggtata attttaattt
     4621 gcttatgagt accattagca attctagata atgctgccat tggttcacct tgagaaccag
     4681 tacatagtat caataactca tgcttcggta cggtattaat tttattaggt tcaataaatg
     4741 tttcaggtgg tgctttaata taaccaagtt ccataccgat tttaatattg ttttccatcg
     4801 aacgaccgaa cgtaacaatt ttacggttat ttttgatagc agcttcaact gcttgttgaa
     4861 ctcggtaaat attagaagcg aaggtagcaa atataatacg acctttacaa ttacggaaga
     4921 ttttatctac gttttgacca acttcacgtt cgcttaaagt aaaatcaggc acaagtgaat
     4981 ttgttgagtc tgaaagtaaa catagaacgc cttcttcgcc taattgagcc attttagcaa
     5041 tgtttgctgg tttgcctaca ggtgtaaaat caaatttaaa gtcaccggta tgaactactt
     5101 ttccttcagg tgtatctacg atgacgccat aagtttcagg aatactatgc gtagttaagt
     5161 agaaagaaat cgtaaagtgc ttagatttta tcacactgtc ctcattgatt tcatttagtt
     5221 tagcagtacg taataaatga tgttcttcaa gtttattacg gattaaacct aatgctaaag
     5281 gaccaccata aataggtata ttaagttgtt ttaataggaa gggcacaccg cctatatggt
     5341 cttcgtgacc atgtgttata aataggccaa caattttatc ttggttttga actagatatg
     5401 tgtagtctgg tataacataa tcaatcccta ataagttatc atcagggaat ttgatacctg
     5461 catcgataat gacaatttcg tctttatact caactgcata agtattttta ccgatttcac
     5521 ctagacctcc aagtgcatat acacctactt catttggatg taattgtttc attatttagc
     5581 aatctccaca ttgaagtgtt ctgagttttc tttttcgtaa tctaaatgtg cgccctctaa
     5641 tttagtgata aattcgatat taaaattacg atctttcaag taacgacgta cttgttcttc
     5701 tgtttgagct tcaacataaa gtgattgtgt attttcacgc acaattacct cgtctctgtt
     5761 atgttgataa aaaactttaa atactgccat gttaaaaatt cctcctaaga atatttgttt
     5821 aatttattgt taaccctagt aaaatcgtat tggagtatat atcgataaat tcattccaat
     5881 catctctata acttaactta ataacgattt ggttaataac taggttcatc atgtcgttca
     5941 ttttaaaaaa ttagtgaaat aac
//
LOCUS       DAHHRR010000138         5953 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.139, whole genome shotgun sequence.
ACCESSION   DAHHRR010000138 DAHHRR010000000
VERSION     DAHHRR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.139"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..1374
                     /locus_tag="NBI42_002328"
     CDS             <1..1374
                     /locus_tag="NBI42_002328"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCD1778719.1"
                     /translation="EDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIE
                     TQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVM
                     INLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGK
                     DKLRHVYDPTCGSGSLLLRVGKETKVYRYFGQERNNTTYNLARMNMLLHDVRYENFDI
                     RNDDTLENPAFLGNTFDAVIANPPYSAKWIADSKFENDERFSGYGKLAPKSKADFAFI
                     QHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSI
                     PTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIINTYKRKETIDKYSY
                     SATLQEIADNDYNLNIPRYVDAFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKE
                     LGVLKDE"
     gene            1367..2602
                     /locus_tag="NBI42_002329"
     CDS             1367..2602
                     /locus_tag="NBI42_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD1778720.1"
                     /translation="MSNTQTKNVPELRFPGFEGEWEEKKLEDLGLFQKSYSFSRAKEG
                     NGKTKHIHYGDIHSKFKTVLDSDGNIPNIIEKAVFELIQKGDIVFADASEDYSDLGKA
                     VMIDFEPNSLISGLHTHLFRPLNNAISNFLIFYTKTLSYKKFIRQQGTGISVLGISKK
                     SLLNLNVLIPRSELEQQKIGQFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRF
                     KDENGNDYPEWENKRIEDIANVNKGFTPSTNNNEYWDNNDKNWLSIAGMNQKYLYKGN
                     KGISKDAAKNYMKVKNDTLIMSFKLTIGKLAIVKAPLYTNEAICHFIWKVNKINTEFI
                     YYYLNSLNISTFGVQAVKGVTLNNDSINSIIVKLPNEEEQNIIAKFLLEVDKTVNNQL
                     VKTKLLKQRKKGLLQRMFV"
     gene            3081..3776
                     /locus_tag="NBI42_002330"
     CDS             3081..3776
                     /locus_tag="NBI42_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HCD1778721.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKAIEQSRLSVTSKDTQ
                     ELKSYYSGTGFNFQNVSGYREENKMNVIVGQQLNVISLLGSDKERFDDGKDYNNLDVF
                     VVREGSGKQADNISIGGITKTNENKYKDTVKHVNMFIYRNTSHNTTTVKSTFHNIYKE
                     EISLKELDFKLRKQLIDNHDLYKTEPKDSKITVTMNNGGYYTFELNKKLQEHRMGDVI
                     DGTKIKEINVELN"
     gene            3798..5303
                     /locus_tag="NBI42_002331"
     CDS             3798..5303
                     /locus_tag="NBI42_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HCD1778722.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTYGEAEASETSDAIGNHNV
                     NKTEESETASQLVQNNKEINADQTKKNTVKLDPIKPGDQKVTGTTLPNQLILLSIDKK
                     DVSSFDSGNSELVMSKEDGKFEYDLNGRKIVHNQEIEVTSSSTEGLFDDEEDEEVEES
                     STEKASVEEESTDAKATYITPRYEKAYKIPNEQLNKENGHHQVFIEPITEGSGIIKGH
                     TSVKGKVALSINNKFINFEESVKGGVSKEDTKASSDGIWMPIDEKGYFNFDFKTKRFD
                     NLELKEGNDISLTFVPEDDEDALKPLIFKTKVTSLEDIDKAETKYDHTKVNKVKVLDN
                     VKEDLHVDEIYGSLYHTDKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEAQLFPDL
                     QVNEKGEFSFDSHGAGFRLQNGEKLNFTVVDPITGKLLSNEFVSKEIDIEETPEQKAD
                     REFDERMENTPAYYKLYGDKIVGFDTNDFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(5409..5717)
                     /gene="spn"
                     /locus_tag="NBI42_002332"
     CDS             complement(5409..5717)
                     /gene="spn"
                     /locus_tag="NBI42_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HCD1778723.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
BASE COUNT         2322 a          802 c         1096 g         1733 t
ORIGIN      
        1 gaagatatca cgtatcaaga agcatgggcg gatgaagaat accgtgaaga cttaaaagca
       61 gaattaattg atcaagtcgg ttatttcatt gagccacaag atttattcag tgcgatgatt
      121 cgtgaaattg aaacgcaaga tttcgatatc gaacatctcg caacggcgat tcgtaaagtt
      181 gaaacatcaa cattaggtga agaaagtgaa aatgacttta tcggactgtt cagcgatatg
      241 gacttaagtt caacgcgttt aggtaacaat gtgaaagaac gtacggcgtt aatttccaaa
      301 gtcatgatta accttgacga tttaccattc gttcacagcg acatggaaat tgatatgtta
      361 ggtgatgcat atgaattcct aatcgggcgc tttgcggcga cagcgggtaa aaaagcaggc
      421 gaattctata caccacaaca agtatctaag atactggcga aaattgtcac agacggtaaa
      481 gataaattac gtcacgtgta tgacccaaca tgtggttcag gttcattact gttacgtgtt
      541 ggtaaagaga ctaaagtgta tcgttatttt ggtcaagaac gtaacaatac cacatacaac
      601 ttagcgcgta tgaatatgtt gttacatgat gtgcgctatg agaacttcga tatccgtaat
      661 gacgacacgt tggaaaatcc agctttttta ggcaatacat ttgatgcggt tatagcgaac
      721 ccaccataca gtgcaaaatg gatagcagat tcaaaatttg aaaatgacga acgcttcagc
      781 ggatacggca aacttgcgcc aaaatccaaa gccgactttg cctttattca acacatggta
      841 cattacttag acgatgaagg taccatggcc gttgtactcc cacatggtgt cttattccgt
      901 ggtgccgcag aaggcgtgat tcgtcgttat ttaattgaag aaaagaacta cttagaagcc
      961 gtgattggtt tgccagcgaa tattttctat gggacaagta ttccaacatg tattttagtg
     1021 tttaaaaaat gtcgccaaca agacgacaac gtattattta tcgatgcatc caatgatttt
     1081 gaaaaaggaa aaaatcaaaa ccatttaagc gatgcccaag tcgaacgcat tattaacaca
     1141 tataagcgta aggaaacaat tgataaatat agctacagcg cgacattaca agagattgcc
     1201 gataacgatt acaacttaaa cataccgaga tatgttgatg cattcgaaga agaagcgcca
     1261 attgatttag atcaagtcca acaagatttg aaaaatatcg acaaagaaat tgcagaaatt
     1321 gaacaagaaa tcaatgcata cctgaaagaa cttggggtgt tgaaagatga gtaatacaca
     1381 aacgaaaaat gtgccagagt tgagattccc agggtttgaa ggcgaatggg aagagaagaa
     1441 gctagaagat cttggcctgt ttcaaaaaag ttattctttt tcgagagcta aagaaggaaa
     1501 cggtaaaact aaacatattc attatggtga tattcattca aagtttaaaa cagtcttaga
     1561 tagtgacggt aatatcccta atataattga gaaagctgta tttgagttga ttcaaaaagg
     1621 agacattgtt tttgcagatg catcagaaga ttatagtgac ctaggaaaag cagttatgat
     1681 agatttcgaa ccgaattcat tgatttctgg cttacataca cacctattta gaccgcttaa
     1741 caatgcaatt tctaattttt tgatttttta cacaaaaact ctgagttata aaaaattcat
     1801 tagacagcaa ggtacaggaa tatcagtact tggtatatca aaaaaaagtt tattaaattt
     1861 gaatgtatta ataccacgaa gtgaattaga acaacaaaaa ataggacaat tctttagcaa
     1921 acttgaccga caaattgaat tagaagaaca aaaacttgaa ttacttcaac aacagaaaaa
     1981 aggctatatg cagaaaatct tttcacaaga attgcgattc aaagatgaga atggtaatga
     2041 ttatccggag tgggagaata agagaataga agatattgcg aatgttaaca aaggatttac
     2101 tcctagcaca aacaataatg aatattggga taacaatgac aaaaattggc tttctatagc
     2161 tggaatgaat caaaaatact tatacaaagg gaataagggg atttcaaagg atgccgcaaa
     2221 aaattatatg aaagtaaaaa atgacacctt aataatgagt tttaaattaa caataggaaa
     2281 attagctata gtgaaagcgc cattatatac taatgaagcg atatgtcatt ttatatggaa
     2341 agtcaataag ataaatacag agtttatcta ttattatctt aattcattaa atattagtac
     2401 gtttggtgta caagcagtta aaggtgtaac tctaaataat gattcgatta atagtataat
     2461 agtaaagtta ccaaatgaag aagaacaaaa cataatcgct aagtttctat tagaagtcga
     2521 taaaacagtt aataatcaat tagtaaaaac gaaattacta aagcaaagaa aaaaaggatt
     2581 attacagagg atgttcgtat aactccaata atatacgagt atgttaatta gataattcga
     2641 aagaatataa tatatcctca taaaataagg atggtgtaaa taattatttc tttataatga
     2701 acaatctgtt gcaattacga atcaggtata tgtaacaact ttgttttata gctgaactca
     2761 taataaacca aatcaaaaac gaacattaaa tatagtcact gtgatagcgc agtgtctttt
     2821 ttgtgtcgaa attgtgtaca gaataagtgg ttaaataaag attaatttga gataaagtgt
     2881 tattcgtaaa taaaagagag tagattgata ggaattgaat gatattagtt aactatttat
     2941 taaattactt aataatgatt aatttttagt taaagtaagt ttaatgtgaa gcacgaccat
     3001 tgctcattat aatgaatgag gattgttcgt attgcgtaat agaataaatc aaatagacta
     3061 aaaattggga gcatagaatt atgaaattaa aaaatattgc taaagcaagt ttagcactag
     3121 ggattttaac aacagggatg atcacaacta ctgctcagcc agtaaaagca attgagcaaa
     3181 gcagattatc agttacttca aaagatacac aagaattaaa gagttattat agtggaactg
     3241 gatttaattt tcaaaatgtc agtggttata gagaagaaaa taaaatgaat gttattgttg
     3301 gacagcaact taatgtaatt tcgttacttg gttcagacaa agaaagattt gacgacggaa
     3361 aagattataa taatctagat gtatttgtag taagagaggg atctggtaaa caggcagata
     3421 atatttcaat tggtggaatt acaaaaacaa atgaaaacaa atataaagat actgttaaac
     3481 atgtaaatat gtttatttat agaaatacaa gccataatac tactactgta aaatctacat
     3541 ttcataacat atataaagaa gaaatatcgt tgaaggaatt agatttcaaa ttaagaaaac
     3601 agttaattga taatcatgat ctttataaaa cagaacctaa agacagcaaa attacagtta
     3661 ctatgaataa tggtggttat tacacatttg aattaaataa aaagttacaa gaacaccgca
     3721 tgggtgatgt aatcgatggt actaaaataa aagaaattaa tgttgaatta aactaataat
     3781 attcgaggga gtaaatcatg agggaaaatt ttaaattacg taaaatgaaa gtcggtttag
     3841 tatctgttgc aattactatg ttatatatca tgacatatgg cgaagcagaa gcatcagaaa
     3901 ctagtgatgc tattggaaat cacaatgtga ataaaacaga agaatctgaa actgcgtcac
     3961 aacttgttca aaataataaa gaaataaatg cggatcaaac aaaaaagaat actgtcaaat
     4021 tggatcctat caagccagga gatcaaaaag tcacaggtac tacattacca aatcaattaa
     4081 ttttattaag catagacaaa aaagatgtga gttcatttga tagtggaaat agtgagctag
     4141 tgatgtcaaa ggaagatgga aaatttgagt atgacttaaa cggaagaaaa attgtccata
     4201 accaagagat tgaagtaact tcatcttcaa cggaaggttt atttgacgat gaagaggatg
     4261 aagaagtgga agaatcctca actgaaaaag caagtgttga ggaagaaagt acagatgcta
     4321 aggctacata tataacacca cgatatgaaa aagcgtataa aataccgaat gaacagttaa
     4381 ataaagaaaa tgggcaccat caagttttta ttgaacctat tactgaagga tcaggcatta
     4441 ttaaaggtca tacttctgta aaaggtaaag tcgccctttc tattaataat aaatttatta
     4501 attttgaaga gagcgttaag ggcggtgtta gtaaagaaga tactaaagct agttcagatg
     4561 gtatctggat gcctattgat gaaaaaggct actttaactt tgacttcaaa acgaaacgtt
     4621 tcgataattt agagttaaaa gaaggtaatg atatctcatt aacatttgta ccggaagatg
     4681 atgaagatgc attaaaacct ttaattttca aaactaaagt aacaagtctt gaagatattg
     4741 ataaagcaga aactaaatat gaccatacta aagtcaacaa agtaaaagtt ttagataatg
     4801 ttaaagaaga tttacatgtt gatgaaattt atggaagttt atatcataca gacaaaggta
     4861 aaggtattct tgataaagaa ggtactaaag taattaaagg gaagactaaa ttcgcaaatg
     4921 cagtagtgaa ggtagactct gaactaggcg aagcacaatt attccctgat ttacaagtaa
     4981 atgaaaaagg tgaatttagc tttgactcac atggtgctgg atttagatta caaaatggag
     5041 aaaaactaaa tttcacagta gttgatccga ttactggtaa gttgttgagt aatgagtttg
     5101 tttctaaaga gattgatatt gaagaaacac ctgaacaaaa agcggatcgt gagtttgatg
     5161 aaagaatgga aaatacaccc gcttactaca agttatatgg tgataaaata gttggtttcg
     5221 atactaatga tttcccgatt acttggttct atccattagg tgaaaagaaa gttgaacgta
     5281 aggcaccaaa attagaaaaa taattaaata aaacagctta atgatgtaat gaaattagtg
     5341 agttaatcac tgacttcaac gtcattgagc tgttttttta tttgcttcgt tacaaagtat
     5401 tagtgaattt attttacgtg ttcatatttt gaaacatcaa agccgtcttg tttagctttg
     5461 ttgataatgt ctttgatgga atgtagtcct ttatcggcga agtatgatct taagttgtct
     5521 tttgtagctt ggtcagcatt cttatctaat aacacatcga tataacttaa ctcgtgttct
     5581 aagaagtttg cgtcatcatg tagtacgagt ccattttgag aataaacttt cgcatctgct
     5641 tgattaccat atccaacaac gccagttgct aaaacaccta ccattgccgt agctactaaa
     5701 acctttttaa atttcatatc tatcactcct ctaaaaattg taactccatc ataacactga
     5761 atattaagaa aattacgttt attaagtcga tttaataatt tttaataaat agttaaactg
     5821 gcaaatattg ctaaaaagca attaaccttt agtgcgatgc ttgtgttcct tagtattgag
     5881 aagtaggttt cgaagatatt ttaattcaaa tgaaacaata ataaaaaaat gatgcaacat
     5941 aataataagt aca
//
LOCUS       DAHHRR010000139         5914 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.140, whole genome shotgun sequence.
ACCESSION   DAHHRR010000139 DAHHRR010000000
VERSION     DAHHRR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.140"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            198..560
                     /locus_tag="NBI42_002333"
     CDS             198..560
                     /locus_tag="NBI42_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HCD1778724.1"
                     /translation="MLEPIKEQEVLELLASYEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHYEVNEHDEFLIAGFN
                     YEGQLAAALEISEQPFNL"
     gene            574..1239
                     /gene="bshB2"
                     /locus_tag="NBI42_002334"
     CDS             574..1239
                     /gene="bshB2"
                     /locus_tag="NBI42_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HCD1778725.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLASYVQRGVPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            1252..2130
                     /locus_tag="NBI42_002335"
     CDS             1252..2130
                     /locus_tag="NBI42_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCD1778726.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            2413..3903
                     /locus_tag="NBI42_002336"
     CDS             2413..3903
                     /locus_tag="NBI42_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1778727.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGNTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKIDVSKRINNEIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITPDFNGYLITKNDED
                     ALFHKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(4027..5499)
                     /locus_tag="NBI42_002337"
     CDS             complement(4027..5499)
                     /locus_tag="NBI42_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1778728.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDLFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFIMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGTGNDIKSFYKTVFNNLTRYKAIVV
                     STEKQYQDISYYIENKIPVINIPVGYVANLKYQFDINQKDKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPPGDINQMVE
                     KVDELLNNTQKLQQFSINSIESAQQFNETNVSTKWQNILN"
     gene            complement(5618..>5914)
                     /locus_tag="NBI42_002338"
     CDS             complement(5618..>5914)
                     /locus_tag="NBI42_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1778729.1"
                     /translation="SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTP
                     VKPMSATKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1990 a          929 c          956 g         2039 t
ORIGIN      
        1 taatgtttaa cactttttaa attccaacaa tttttttgat ttttaaaagt aaaaaactaa
       61 tgctgtatga ttgtgtaatc ctgagtaatt acccttcatg aaaattaatt tataaataat
      121 tgagcaatcg tatacaatta ttgaatagtt tttagtaaaa tagaaagata aacacattga
      181 tagtgtaagg agtgaacgtg ttggaaccaa ttaaagaaca agaagtttta gaattactag
      241 cttcttatga acataaacct gtgtatctac atgttgaaac gacaaatggt gcctatgcga
      301 accatttcga tcaacgtgtt tttaatgctg gtactttttt aagaaacatt caaataacat
      361 acacacatgc tcagttaaaa ggtggcaata aagaacctta tcgcattggc cttaaactat
      421 caaatggtgg ctgggtttat gtacaaggtc taacgcatta cgaagttaac gagcatgacg
      481 aatttttaat tgcaggtttt aattatgaag gtcaactcgc tgcagcgctt gaaattagcg
      541 agcaaccatt taatttatag aggaggcatc aacatgactg atgaaagaca tgtattagta
      601 atatttccac atcctgatga tgaaaccttc tcatctgcag ggacactagc aagttatgtc
      661 caacgaggcg taccagtaac atatgcatgt ttaactttag gtcaaatggg acgaaattta
      721 ggtaacccac catttgcaac tcgtgaatca ttaccaagca ttcgcgaacg tgaacttgaa
      781 gaagcatgta aagttattgg cattactgat ttaagaaaaa tgggtttacg cgacaaaaca
      841 gtcgaatttg aaccttatga acatatagat ggtatgatta aatctttaat cgatgataca
      901 aatccatcat tgattatttc attttatcct ggatatgcag tacatcctga tcacgaagca
      961 acagcagacg ctgtaattcg aacggttgaa cgcatgccta aagaagaacg ccctcgttta
     1021 actttagttg cgttcagtaa tgatgcaact gaagctcttg gagagcctga tattcaaaat
     1081 gatattacag attttaaaga acttaaaatt aaggcatttg aagcacatgc ttctcaaaca
     1141 ggtccatttt taaaacaatt agcaagtcct gaaatcgatg gtaaacaaca cagcttttta
     1201 acagtcgaac catattggac atatcacttt aaatcttaac ggaggaaaaa aatgactgaa
     1261 tttgatttat cgacacgaga aggtcgttgg aaacactttg gttccgtaga tcctattgaa
     1321 ggcacgaaac ctactactaa gaacgaaatg acagatttac aaagtacaca taaagatttc
     1381 ttatttgaaa tagaagaagt tggtattaaa aacttagtgt accctgtact tgttgaccag
     1441 tatcaaacag ctggcacatt tagtttttca acaagtttaa caaaagacga aaaaggtatt
     1501 aatatgagcc gtattattga aagtgttgaa aagcactatg ataatggtat tgaacttgaa
     1561 tttaatacac tttatcaggt gttacgcact ttacaaacga atatgaaaca aaatgcggca
     1621 ggcgttgatg tttctgggaa atggttcttt gatcgctata gtccaaccac aaatattaaa
     1681 gcagttggta atgccgatgt cacttatgga ttagcaattg acggtgataa agttacacgt
     1741 aaagaactta ctattgaagc aactgtcaca acgctatgcc cttgttcaaa agaaataagt
     1801 gagtattcag cgcacaacca acggggtgtt gtcactgtta aaacatatat taataaagat
     1861 caagacatag ttgatgacta taaaaataaa attttagatg caatggaagc taatgcaagt
     1921 tcaattctat accctatttt aaaacgtcct gatgaaaaac gtgtgacaga gcgtgcatat
     1981 gaaaatccac gttttgtcga agacttaatt cgtttaattg ccgcggactt agttgaattt
     2041 gattggttag acggtttcga tatcgaatgt cgtaatgaag agtctatcca tcaacatgat
     2101 gctttcgcta aattaaagta tcgtaaataa tatcatatga ttaattcaaa cgcgtaacag
     2161 tagttgcgcg ttttttatta tacctcctag agttgtatag ctcttttata aatcttctta
     2221 tttgatgtac ctgagtcata aatcaaattc ctaagctcta caatgatata ttggtcgtag
     2281 ttgactgaat aactactacc attataaata tcaaaacttg ttgttttata aataaacaca
     2341 tcttccagtt aaaagaagta attaaatatt atgtgatata attataaaaa atagatataa
     2401 gtggtgttgt aaatgaatta ttttgtaggt aatagtttag gtgttaattt aactgggata
     2461 gagaaagcaa ttattaatcg tttaaattta tttaaagaaa tgggaagacc agcacaatgt
     2521 gtatttctgt catggaatag atatttatat agaaatgccc aaaattatat cactagttct
     2581 gactatatta atatgtatga tttcttccaa gaagcaacat atttagaacg caatgaacca
     2641 tttgactggt tgtcatattg gactgatgaa tgtcattata ctttaaaaca tgttgaaaat
     2701 agtcatgact ttagaatata tgatcaagag cgtttcttaa tgtatgccca ttttcaagat
     2761 cctaaatatc gtatattaga ttatgtgaat cattttgata gtcaaagacg aaaagtaaaa
     2821 cgtgattttt atgacgtgcg tggcttttta agttgtagta ggattcttgt tgataaacaa
     2881 caaacattat gtgaattttt ctataatcca gaaggtaaca caaaattaga aaagtatttt
     2941 tcttacaaag atggcaaacc agaagtacaa aaaattattg tttactatgc taacaaacaa
     3001 tatttcttta ataatgaaac ggaattaggc gcatttttca ttaaacaact atatcaacat
     3061 ggggatttat ttttcagtga tcgtaatgtg tatacagcac ctattttcaa cttaacacct
     3121 gagtcaatcc cagtagtagc tgtgcttcac agtacacaca ttaaaaatat tgatgcatta
     3181 gattcatcgc catttaaaaa tgtatataaa gcgatgtttg aaaatttatc tcgatatcgt
     3241 gcaattatcg tttctacaga acagcaaaaa atcgatgttt caaaacgtat taataatgaa
     3301 attcctgttg ttaatatacc tgtcggatac agtgaaacaa ttgatacacc agtccaaaca
     3361 ttagatcagc gcagtgttaa attaatttcc gttgctcgtt attcacctga aaaacaatta
     3421 catcaacaaa ttgaattaat taaacgctta gtatcttatg tccctaaaat agagcttcat
     3481 atgtatggtt ttggttctga aagtaaaaaa ttaaatgaat tgatacaaaa atatggattg
     3541 gaaaatcatg tttacttacg cggcttttta agcaatctag accaagaata tagcgatgcc
     3601 tatttgagtt taataacaag taacatggaa ggtttctctt tagcactact tgaatcttta
     3661 gcacatggtg ttccagttat tagttatgat attaaatatg gacctaacga attgattacg
     3721 cctgatttca atggttattt aattactaaa aatgatgaag atgccctatt tcataaagta
     3781 aaatatgtga ttgatcatcc tgaagtacaa caacgactgt caaaaggtag tttggcaaaa
     3841 gcgcaacaat atagtaaagc gtcgttaatt aaacaatggg accaatttgt tcgtcttata
     3901 taaatgtact ttacctttat ttttataaaa aaagcatttt cctatgtcat ttaactaaac
     3961 ttgtaagttc gtatgaacgt ggtttgttaa atagatgatt ctaggaaaat gcttttttat
     4021 tttgacttag tttaaaatat tttgccactt tgtactgaca tttgtctcat taaattgctg
     4081 agcagattct atactattaa tcgaaaactg ttgcaatttt tgagtattat ttagtaattc
     4141 atcgaccttt tcaaccattt gattgatgtc acctggaggt actaaatagc cattaaatcc
     4201 atcttgaatc agttctgatg gcccataatc tacgtcataa ctgatcactg gtgtacctac
     4261 tgaaagcgac tctaaaattg ctaatccaaa accttccatt ttacttgtcg ataacatcag
     4321 ttctgcttta gcaatttctt cattaatatg cgtcttaaaa ccatgaaatt taacatgttc
     4381 cgataaatga taatcttcta caagttgtcg atattctgac aaaccatttc catgtccata
     4441 aatattcaat tgaatattgg gatgttttgt tactaattgc ttgatcactt caatttgatg
     4501 tttaatttgt ttattttcaa cgaggcgtgc aattgatatg atatgatttt tatccttttg
     4561 attgatgtca aattgatact ttaaatttgc cacgtagcca accggaatat tgataactgg
     4621 tattttattt tcaatataat atgaaatatc ttggtattgc ttttctgttg atacaacaat
     4681 cgctttataa cgtgttaaat tattaaacac tgttttataa aaacttttta tatcattacc
     4741 ggtgccggat aaatgtgtac tgtggagcac aacaataact ggaatacttt gatttaatcc
     4801 cgcaataaca tttcctaatt catgaggacg gtctagtatg atttgatcat tatttttaca
     4861 taattgatgg agaaaatatt gaactaattc atcttctgta tcaaaaaatt gttgatgctg
     4921 gtcttcattt aagataacct ttgtgagcgt gtttttccct tttatatcgt cgaaatattt
     4981 ttggatgacg atttccccat taggcgtata gtaattttcg agtacaatcc gttgcccttc
     5041 acctaaaatt ctagaacaac ttaaaaagcc tcttccatca tacaattcac gttttacttt
     5101 tcttctttta tgatcaaaat aattcacata gtttaattga tgatactgtt tatctaaaaa
     5161 atgagcatac attataaatt gctcttcatc atatattctg acatcatttg aattttcaac
     5221 aaatttcaat gtgtacctac atgacttttc ccaatactgt atccagttaa cttgctttgt
     5281 ctttttataa ttgattgctt tttgaaaata gtcatacatt gtaaacagat cattttcaat
     5341 ctgatgttgc ttcgcatatg tgtatgaata aggattccat ttaacatata cacattttga
     5401 agatatgccg tgttgtttga acaacttcag tctatttatt tgtgcttttt ctacacctgt
     5461 aattttactt tctaaaattg ttcctaaaat gtaattcata ttatcgcctc atataagttt
     5521 tattacgtat ctttattgtt tattttatat gaaaaataca tctattgcat gtgtaatcat
     5581 aaaaaaacca ggccacaagg acctgggtca tattgtatta tttgttttgt tttttgcgac
     5641 gaccgaataa caataatgaa cctaatgctg cgaataatcc gccaaataac gtcgcgttat
     5701 ttgagccgtt attttcacta cctgtttctg gtaatgcttt cgctttattg tgatggtctt
     5761 tagtagcact cattggttta acaggtgtat gttttcctgc atctgaatct gaatcactat
     5821 ctgaatctga gtcactgtct gagtctgaat cgctatctga atctgagtcg ctatctgaat
     5881 ctgaatcgct gtctgagtct gagtcgctat ctga
//
LOCUS       DAHHRR010000140         5897 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.141, whole genome shotgun sequence.
ACCESSION   DAHHRR010000140 DAHHRR010000000
VERSION     DAHHRR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.141"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            159..1352
                     /gene="fmtA"
                     /locus_tag="NBI42_002339"
     CDS             159..1352
                     /gene="fmtA"
                     /locus_tag="NBI42_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HCD1778730.1"
                     /translation="MKFNKVKLVIHVCVLLFIIISIALIFHRLQTKTHSIEPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNSLLHEFGTKQYPDEYRYGFY
                     VKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(1889..2167)
                     /gene="qoxD"
                     /locus_tag="NBI42_002340"
     CDS             complement(1889..2167)
                     /gene="qoxD"
                     /locus_tag="NBI42_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HCD1778731.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(2176..2781)
                     /gene="qoxC"
                     /locus_tag="NBI42_002341"
     CDS             complement(2176..2781)
                     /gene="qoxC"
                     /locus_tag="NBI42_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HCD1778732.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(2771..4759)
                     /gene="qoxB"
                     /locus_tag="NBI42_002342"
     CDS             complement(2771..4759)
                     /gene="qoxB"
                     /locus_tag="NBI42_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HCD1778733.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(4759..5859)
                     /gene="qoxA"
                     /locus_tag="NBI42_002343"
     CDS             complement(4759..5859)
                     /gene="qoxA"
                     /locus_tag="NBI42_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HCD1778734.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
BASE COUNT         2113 a          952 c          995 g         1837 t
ORIGIN      
        1 tttgttgtat agataacata ttagaaaaac ttattactat gtagcgtaaa gcataaatga
       61 atgataaatt aatttttgta agttgtatta ttaggaatct aggttcaaag ttttaactat
      121 ttaaatatat aatgaataca ttgtgaggct attatcaaat gaaatttaat aaagtaaaac
      181 tagttataca tgtgtgtgta ctattattta tcattatttc tatagcgtta atttttcatc
      241 gattacagac gaagacacat tctatagaac caatacataa ggaaacaaaa ttatcagaca
      301 atgaaaaata tttagtagat cgtaataagg aaaaggttgc gccgtctaaa ctaaaagaag
      361 tatataatag caaggatcct aaatataaga aaattgacaa gtatttacaa agttcattat
      421 ttaacggttc agtagctata tatgaaaatg gcaaattgaa aatgagtaaa ggttatggat
      481 atcaagattt tgaaaaaggt attaaaaaca caccgaatac gatgttttta ataggttcag
      541 ctcaaaaatt ttcaacaggg ttactgttaa aacagttaga agaagaacat aaaataaata
      601 tcaatgatcc agtaagtaaa taccttccat ggtttaaaac atctaagcct atcccattga
      661 aagatttgat gttgcatcaa agtggattat ataaatataa atcctcaaaa gattataaaa
      721 acttagatca agcagttaaa gcgattcaaa aacgtggtat tgatcctaag aaatacaaaa
      781 agcatatgta taacgatggg aattatttag tacttgctaa agttattgaa gaagttacag
      841 gcaaatctta tgctgagaat tattatacaa aaataggaga tccattaaaa cttcagcaca
      901 cagcatttta tgatgaacaa ccttttaaaa aatatctagc aaaaggttat gcttataata
      961 gtacaggact ttcattctta agacctaata ttttggatca atactatggt gcaggtaatt
     1021 tatatatgac accaacagat atgggtaaat taattactca aatacaacaa tataaattat
     1081 tcagtcctaa aataaccaat tcattattac atgagtttgg tacgaaacag tatccagatg
     1141 aatatcgata tggtttttat gttaaaccaa cattaaatcg tttaaatggt ggattcttcg
     1201 gacaagtctt tactgtttac tataatgata agtatgtagt tgtacttgca ttaaatgtaa
     1261 aaggaaacaa tgaagttcga atcaaacata tttataatga tattttaaaa caaaataaac
     1321 cttacaatac gaagggtgtt attgttcaat aattaatata gaagatataa catgtatatg
     1381 tcattaaggc atcgacctta tctgaccagt atacgagtta tatcttcttt tttatagtgg
     1441 taaaaagttt aaagtataag gttgaagaag gatgagttta aaaatttgtg ttaactgata
     1501 aaaggggaaa acatttgatg agctggcatc aaactaaaat gaatgaagac gaatacgttg
     1561 gtccatgcgt ggtctgaatg ttttctaaat ctaactggaa atgataattt aataatctac
     1621 atgtttgatt gtttaaacat gtacaatttt taaaaaccta gggataattt tattgaggtg
     1681 aacaacattg aaattatctt tagacataaa tatggatttt gaagttacta tattaattag
     1741 ttgtggttgt taatgtatta attgaattga taataatgat tgtcttataa gatatagaag
     1801 gggacttaac catttaaaaa ttgaaggata ataaaaacca gctaacaaaa gtgttagctg
     1861 gttaaacttt acattattaa aatgttgttt ataagtgtga agagtgaccg ccttgcataa
     1921 cccaatatgt tccgacaacg aaacaaagtg taattacaag agcaaagata actttgaatg
     1981 tttgtaaacg tccatcttta ccttcagtta aatgcatgaa cattaataat tgaagtcctg
     2041 cttggacgaa tgcaaagcca aagataattg tcaacttcgc gtggaatgtt aatgacgtgt
     2101 atagtgttac gtaaactgct aaaagcgtta atacgataga tgcgataaat cctacagtat
     2161 gtttcattat tgtactcatc cgctatacac catccctatc atatatacgg cagtaaagat
     2221 gaaaacccaa acaacatcta agaagtgcca gtataaactt actataaata attttggcgc
     2281 attatatttg tctaatccgc gtcgttggat ttggattaat aaacaaatgg cccaaacgat
     2341 acctagcgat acgtgacaac catgcgttcc taataggata aagaaactag accagtaaga
     2401 accaattgtt gggttaacgc cttctgatgc atagtgtgcg aattcataaa tttcgaatcc
     2461 aacaaagact aaacctaaaa gtaacgtaat gatcatccaa aacatcatta acttttgttt
     2521 ttcttggcgc atgtagtaaa tagcaatacc acatgtgtaa gaactgaata ataatgcaaa
     2581 cgtcattatt aaaacaagag gcaattcaaa taactcagta gtcattttac ctgcataatc
     2641 gccaccatgt tgcaaagtta atagtgtcgc aaatagggta ccgaataacg caaattcggc
     2701 tgtaatgaaa atccaaaagc caagcttatt taattcgcct tcatgtgtgc gtgaatcaat
     2761 agtgtttgta tcatgactca tgacttacag cctccctttc tttaatacgc gcttctctta
     2821 atctagcttc agtttcagca acttcagcag cagggatatg atatccgtga tcgatttgga
     2881 aactgcgata aatcatagta ccaaaaatac cgaataaaca aattaatgct ggaattacag
     2941 tttcgaaaat taagaagaaa ccgccgatag tcataaagat accaatccag aatccaacag
     3001 gagtattgtt tggcatatga atgtctttgt aattatggtt gtctaagtaa tgacgaccat
     3061 gttctttcat atcaacaaat gtgtcgtagt cattccaatc tggtgtaatg gcaaagttgt
     3121 atttaggtgg aattgctgat gcagtagtcc actctagagt acgaccaagg ccatcccagt
     3181 tatctccagt tgcttcacgt ggagatttga agtgactgta tacgatacta acaacaagga
     3241 ataagaatcc gattgccatt aataatgcac cgatagttga aattaagttt aataagaacc
     3301 aaccatctga tggcatataa gtgtataaac gacgtggcat accatctaat ccaagaatga
     3361 attgtggtaa gaaacaaacg ttaaatccga tcatgaagaa ccagaagcac catttgttta
     3421 atgtttcgtt taatttgtaa cccatcatct ttggatacca gaagattaat ccagctaagc
     3481 aggcaaatac aacaccagta accaatgtat agtggaagtg agctactaag aagtacgtat
     3541 tgtgatattg atagtcagca gatgccattg ctaacataac acccgtaaca ccacctaaaa
     3601 ggaagttagg gataaatgct aatgagaata acattggtga ctcaaatgta atacgtcctt
     3661 tatataatgt taataaccag ttaaacaatt tcacaccagt tggaatacca atcagcattg
     3721 ttgaaattga gaagaatgag ttgattaacg caccattacc cattgtgaag aaatggtgaa
     3781 cccaaactaa gaaactaagg aacgcgatac cggcagttgc ccataccata ctttgatgtc
     3841 cgaataaacg cttacgagcg aatgtcggaa taatttctga gtaaatacca aatgccggaa
     3901 ggataacgat ataaacttca gggtgccccc atacccagaa gaagttagcc caaagcattg
     3961 gcataccgcc atgtgcaact gtgaagaatg ctgtgtcaaa tattctatca gttgtcatta
     4021 atgctaacgc tactgttaaa ggagggaaag caagaataac aattaatgta gtaataaatg
     4081 ttgttactgt aaacattggc atttgcataa acttcatagt tggtgtttta catcttaaaa
     4141 ttgtaacaaa gaagttgata cctgtagcta aggtaccaag ccctgaaatt tgtatagcta
     4201 ttaagtaata gttaacaccc ggaccaggac tgaattcacc tgctagtggc gcatagtttg
     4261 tccaaccagc tgctggtgaa ccaccaataa taaatgacag gttgaataaa atcatacctg
     4321 caaagaatag ccagaaactt acgttgttta atactgggaa tgcaacatca cgtgctccaa
     4381 tttgtaatgg aacaacgata ttccataaac caaagataaa tggcattgcc atgaagataa
     4441 tcatgattac accatgtgta ctaaaaattt cgttatagtg gttagattct aaaaatttgt
     4501 tatcaggtac tgttaattgc gcacgaataa gtaacgcatc aataccacca cggacgaaca
     4561 ttaatacggc acagattaaa tacataatac cgattttctt atggtctaca gatgtgaacc
     4621 attctttgta aagatatttc cataatttaa agtaagtaat tactgcgatt aaaccaataa
     4681 ctaagaatgg ggcaccaatt tgtgccattg taatcatcca gttaccttta actagtaatt
     4741 gatcccatgg aaaattcatt aatgtccacc tccatgatca tcattgtctt gatcttgcgc
     4801 atcttttgaa attttcttca tttctttcgc atttttcgat tcatctttct tgaactcatt
     4861 gttatatggt tcgtcatttc caagaatcat caatttcata ccatgtcgtt tatagttcgc
     4921 atttgtaatt tgagctttac gagcaggtat taatggtttg tctgatacat ctttaaacat
     4981 attttcttca ctagtgaagt ttggatcttt caattcgaaa ttgaaacgtt tatatgcata
     5041 gaagatgtat tctggatcgg ctgctggatc aacaaacgcc atatgtgtac cattaaattc
     5101 taaagcttta ttaggtgtgc ttggtaataa ttgtttatca aatgtatctt gatctaacgt
     5161 tttcttacct ttaacttctt tcacccattt gtcgtagtct ttttgactaa cggcatttac
     5221 tttaaatgtt tgacgtgaga atccttcacc attgaagtta gagttacgac ctctgaacgt
     5281 accagtttga gatgcttcta acgtccaatt cattgtcatg ccagtcatgg catatttttg
     5341 accacctaat tgtggaatcc agaaacttgt cattgtatcc atagcttgaa gcttaaatac
     5401 aacaggacga tctttaggga ttgttaatgt attaacagtc tctatatgtt catctggata
     5461 agcaaagaac catttgtatc ctgcacttac tgcatataca accattggat ctttctcact
     5521 cttcggtggt ttttcgtaat cgtataaagt tttaactgta ggaatagcta aagcagcaac
     5581 gattatgata ggtattacaa accatattgt ttcaatgatg gcattatggt gcatcttacc
     5641 agattcggca ttcttattat aactatactt gtaaataaaa atggcgaaca tgccaagtac
     5701 aacgaaacaa ataacaagca tgaagacgat tgaataaaga atcaagaact tctgactact
     5761 tgctactggc ccttttgcgt tgaaaatttc tatatttgaa caaccactaa gtaaaattag
     5821 tgtgccaaat aatagaagca aagacttaaa ttttgacact tttttgacct cctaatacta
     5881 caaatgtagg gcttaac
//
LOCUS       DAHHRR010000141         5768 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.142, whole genome shotgun sequence.
ACCESSION   DAHHRR010000141 DAHHRR010000000
VERSION     DAHHRR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.142"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..327)
                     /locus_tag="NBI42_002344"
     CDS             complement(<1..327)
                     /locus_tag="NBI42_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCD1778735.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKAFLNESRELTTVDFEFFSRDILEDFKGVDRNDIEIRQQRIILH
                     KGEEMIE"
     gene            complement(245..544)
                     /locus_tag="NBI42_002345"
     CDS             complement(245..544)
                     /locus_tag="NBI42_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HCD1778736.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            complement(531..977)
                     /locus_tag="NBI42_002346"
     CDS             complement(531..977)
                     /locus_tag="NBI42_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCD1778737.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTFKGLSNLRVI
                     DDEANIISLITELNYIKSQAIANQGYINVRFYENSDTIKVIENNKIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIDNNNSIYRIIFHIEKGRIRYEKL"
     gene            complement(955..1266)
                     /locus_tag="NBI42_002347"
     CDS             complement(955..1266)
                     /locus_tag="NBI42_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCD1778738.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            complement(1280..2350)
                     /locus_tag="NBI42_002348"
     CDS             complement(1280..2350)
                     /locus_tag="NBI42_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCD1778739.1"
                     /translation="MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMAYKNKQLGATILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            complement(2322..3296)
                     /locus_tag="NBI42_002349"
     CDS             complement(2322..3296)
                     /locus_tag="NBI42_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCD1778740.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI"
     gene            complement(3348..3971)
                     /locus_tag="NBI42_002350"
     CDS             complement(3348..3971)
                     /locus_tag="NBI42_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1778741.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLADSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(3968..4297)
                     /locus_tag="NBI42_002351"
     CDS             complement(3968..4297)
                     /locus_tag="NBI42_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCD1778742.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(4297..5283)
                     /locus_tag="NBI42_002352"
     CDS             complement(4297..5283)
                     /locus_tag="NBI42_002352"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCD1778743.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGFTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVDFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFTPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(5280..5483)
                     /locus_tag="NBI42_002353"
     CDS             complement(5280..5483)
                     /locus_tag="NBI42_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCD1778744.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(5464..>5768)
                     /locus_tag="NBI42_002354"
     CDS             complement(5464..>5768)
                     /locus_tag="NBI42_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778745.1"
                     /translation="NIVKQTINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMF
                     PKSGLLNFELAIANRSLNDDEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
BASE COUNT         1868 a         1015 c          738 g         2147 t
ORIGIN      
        1 ttcgatcatt tcttcacctt tatgtaaaat gatacgctgt tgtctaattt caatatcgtt
       61 tctatctaca cctttaaaat cctctagaat atctcttgag aaaaattcga aatctacagt
      121 tgttaattcc ctactttcat ttagaaaagc tttgctaagt ctaattaagt ctggaacaat
      181 tagtaaagtt atacttataa ccatcatcgc tactaacatt tcaataagcg aaaaagcttt
      241 gacattaata ctgtatacat gtcttttgat aagaagtggt attttttgaa atagcacaaa
      301 tttgttggtc actttgctta atatcatatt tccctatagt tacccccttc ttaagttctt
      361 ctttattaat tttagataca gtagtcaaaa ttactttaga agcatcaatt gtttgtagtt
      421 tatggtttat actcgcttgc atttgattca tcattggtat caatagtaat gtaatcaatc
      481 caattagcaa aaatccagcc atactatcta ttaagaatga tcctttacac ttatagcttt
      541 tcataacgaa ttcttccttt ttcaatatga aatattattc tataaattga attgttattg
      601 tcaattgtta tgctaccaaa tttattgata ttcccctttt tatcaaaggc aataatatca
      661 acttttgcaa cattaattat tttgcctact tttaatttta gaaatcgtat tttattattc
      721 tctattactt taatagtgtc actgttttca taaaatctaa cattgatata tccttgattt
      781 gctatagctt gcgacttaat ataattcaat tcagtaataa gagaaatgat atttgcctca
      841 tcatctatta ctctaagatt gcttaatcct ttaaatgtca ttgtcaaaag taaaaatata
      901 ctgattaaca tcattaccac aagcatctca atcatagtaa atgctgactg ctttctaatt
      961 tgcaactgct tctccattac taattgttat tgtctctcct gatttacatg tcttttgtgc
     1021 ttcttttata aaaccatctg caattaagtc ttcaatagac gatggatttc tattatgttt
     1081 caatgcatac gcttcaattt gactattaac catttttacc tgtgcattac aacctgttga
     1141 ttgtatgtga gcagtttgtt tagcaatatt tggaatgatt aaaattaata ataaactgat
     1201 gattaataac actaatagca tctctatcaa tgtaaacgct tgagttttct taagaaattt
     1261 aaacatatat taacctccat tattttatac tttgcatcat ttgaaacatt ggtaacataa
     1321 ttactaaata aattgcgaca ataaataaac ctaaaatcaa aaataaaata ggctgtaaaa
     1381 actgagtctg ttttatcgct ttatcttcta tttgttttac taatatttgc gaatataact
     1441 ttagttctac ttctagcttc cctctctttt caccttgtag cacaaactta attaattgag
     1501 gcttaaagca ttttagtttt tctaaaattt gaggtaaacc atatcccatt tctgaataag
     1561 ttaataagta tttaccgaga aactgtctaa atggatcact actatggtta atataaacgt
     1621 ctactattga ttgaagtgta ataccatttt tataaaacaa cactaattca ttagttacaa
     1681 aataagtttt aaataattgg aaatagcctg atattagcgg tagtttcatc acaaagttta
     1741 tcttattgag catattcaat ttgttataaa ttaatttcat aataatagcc aacatagata
     1801 ctattatgag cattactaca attatagtag gtaagctggt aatgaaaaaa gacaatgttt
     1861 tttgaaaaga agatagttga atattcatag aagtatataa ttgttgaaac tgtggaatta
     1921 ctgtgaggtt taatataata atcatagcaa taaaaataga aactagtatt aaggggtatt
     1981 gcagtgtctt taacaatcgt tgttctgact ttctatttac tttcatataa tttacggttt
     2041 cttctagaac gtctataata ttgccaaatc tttctgccaa atatacttgc atgacgatag
     2101 tatcgctata acctatcagt gataatatct gattgcatgg tgcaccattt gaaatttcac
     2161 ttagaatggt ggcacctaat tgcttatttt tatatgccat ttgaagattt aaaaattgaa
     2221 aactttgata cagagtgaaa ccatatttca acaaattaca taaatttgaa agtaaatcga
     2281 tttgttgggc cttacttaat tgccgcttct tagaatgtag tttaaatgta tttatccatt
     2341 gtagtttcac aaatgacacc tgcttttgtc atatcatcaa gtttatcttc taagttctta
     2401 aatgatgatg gtaatgaatg attatgggaa aagaaatatc ggagttgttg ctgagataga
     2461 atttcacata ctaattgtcg ctgttgctta atagtagtta caagtcgttg gtttataatt
     2521 aagttagttg cctgtatcaa ttcttgtaca gaaatgccca tttctaatag ccttaaaata
     2581 gcacctttac aatcagttgc atgcaatgta gtcagaacaa ggtgaccact taaactagcc
     2641 tgtataacac acttggcaac atctttatct ctgatttcac ctattaaaat aacatcagga
     2701 tcacatctta aaatagcttt aaacgaattt acatagttaa tgccagcttt atcattcaca
     2761 ttaatttgga cgataccagg aatttgcatc tctacaggat cctctataga aattacattt
     2821 aaattcaagg ctttattcgc gtatgagacc atttgataca ttaatgtact ctttcctgaa
     2881 ccagttggcc cactaaacaa tagtaatcct tgtttcttat tcatgaggtg tttaaaatca
     2941 ttgaatttat aagttgattt ctgttgttga aaaaattgag gtacaattct gataacacaa
     3001 ctttcttggc caagtgacaa tggcaaagtt gatattctca aaaaatatat tttattgaaa
     3061 aggtaactat atcgaccgct ctgtgcgact tgctgtgtag aaacatcaag cccagcttga
     3121 aacttcatat aaactaataa cttttgataa atgctattcc caatttgttc atactgctcc
     3181 aagttatcat taattctaaa tttaatactt acttcatttt taactggaat aaaatgtaca
     3241 tcactcgctt tcatttctat cgctttatta attatttctt gaaatagaat cttcaaaaaa
     3301 acacctccta catataatca cgtaggaggt atttttatta cttcaattta accgtgtaaa
     3361 aatggattta attgttcatc atcaaccgtc gtatatggac catgtccagg gaataaaggt
     3421 aaatcgcctt ctaattcaaa tattttatct tgaatagaat cagcgagcgt ttcataatca
     3481 cctttatata aatctgtacg tccgattcca ttattaaata atgtatctcc aacaactgcg
     3541 aattcatcga acacatatgt taaacttcct ggtgaatgtc caggtgtgtg taacacatta
     3601 aacttaaatc cttctatttc tgtgctacct tcgtttaact tttcaggagt taccttactt
     3661 gtaataattg gtaatccata ttgcttaaat ttatctgctc catttttaac gggatctttt
     3721 agaaaatcaa actctgcttc atgcatataa accgggacat cgaatcgatc aactatatca
     3781 tcgactgctc cgatatgatc aaagtgtgca tgtgttaata aaatagcttt taacggttta
     3841 tttatttggt ttaatttttt aataattttt tcactttcac ctgaagggtc aatcaggata
     3901 acagctttgt cattttcgat gaaatacgta ttagtatcaa ctaagcctaa agttaagctt
     3961 gaaatcctca taggttttca ccactatttt ctaaatgttt ttgtactgat gttagtttat
     4021 cattcatttt tctagatttg tctcgtcggt catcaatacg gatatttgta caaactctac
     4081 ttaaaccttt atcaaaaggt aattcatgta tcacttgcac aacttctaat acatcgctta
     4141 attcaccttc aattagagta ttcattggtg ttaattgaaa atcaatttta cccatcgctt
     4201 tatattcttg aagttttttc tgaatatctg caatatattt actaacactc ggaccttccg
     4261 ttccaactgg aataacaacc acatcaacaa tagccattat tttaccccct ctttatctaa
     4321 tacataggtc ttgattaatc ctgctgctcc tgtaatacct gcatcattac ctaatttcgc
     4381 ttgtacaatt tcagtttcaa attgagcagg tgtaaatgtt aaattatgat attctgtttt
     4441 aatattttca attaaaatag gtcctgcagt agacattcct ccacctagaa cgatatattt
     4501 cggattactt gtaacactaa taatactaca taaatatcca atatagtttg caaccttttc
     4561 agtaatgaaa atacagaatt ggtcaccagc ttttgccgca tcaaaaacag cttttgctgt
     4621 aaccttattt tctttaatca attctaatat agaagatcta aacgtcaact tcggatagta
     4681 gaagtcgact aagttaacaa cgcctgtcgc tgaagcaact gtttcaatac atccagaacg
     4741 accacaatta catttaaatc gttgatcgaa gtctgctcta aaatgaccta tttctgcgcc
     4801 agagccatta tgaccatgta cgatttcacc atttgaaata attcctccac ccagtcctgt
     4861 accaagtgtg atggcaacaa catcatcggc accttcacca gcacctttgt gtttctcccc
     4921 taaagcagct atgttagcat cattatctac atacactgga caatcaacga attgttcaaa
     4981 aatctcacgt acattaactt tttctggcca atataagttt actgctccat ttactgtacc
     5041 tttttcaaag tcaacaggac ctggtacacc aatacctacg ccaagtacat ttgaaaaatt
     5101 ataattattt tcatttactt tttcaacaaa cgaatcataa attcctttca aaagtgtaaa
     5161 tcctgtacta tcagatgtat cagtgtgaat agaccattta tgtaattgtt ctaattcagg
     5221 tgtgaaaata cctaatttac aagtcgtccc gcctacatca gctgctaaaa taatttttgt
     5281 catttctgtt cattccttct ctgattaatt atcaacgtac atttcaaata ttcttcctta
     5341 gataacagtt catattgata caatgatgaa atctcctgtt gaatcatttc gtacatatct
     5401 tctggatttt taaaatatat tagaaatccg taacttttca ataactgttg tacatcataa
     5461 aagttattta tttttcgact catttgattt agtcaactct ttttctaagt taatataatc
     5521 tgtattttta ggatcagcat ttaatgcttt acgcacgtat tttaatgctt tttcatcatc
     5581 atttaatgaa cgatttgcta tcgctaactc aaaatttaat aaacctgatt taggaaacat
     5641 tctaagtcct cgctcccatt ctgtcatacc ttcagactta gaattgatag tagccataat
     5701 cataccactt aaataatatg tttgatcatc ggcataattt ttatttattg tctgcttcac
     5761 aatatttt
//
LOCUS       DAHHRR010000142         5623 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.143, whole genome shotgun sequence.
ACCESSION   DAHHRR010000142 DAHHRR010000000
VERSION     DAHHRR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.143"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            359..1270
                     /gene="rapZ"
                     /locus_tag="NBI42_002355"
     CDS             359..1270
                     /gene="rapZ"
                     /locus_tag="NBI42_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCD1778746.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1267..2262
                     /locus_tag="NBI42_002356"
     CDS             1267..2262
                     /locus_tag="NBI42_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCD1778747.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQSFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2374..3318
                     /gene="whiA"
                     /locus_tag="NBI42_002357"
     CDS             2374..3318
                     /gene="whiA"
                     /locus_tag="NBI42_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCD1778748.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            3888..4475
                     /gene="clpP"
                     /locus_tag="NBI42_002358"
     CDS             3888..4475
                     /gene="clpP"
                     /locus_tag="NBI42_002358"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCD1778749.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(4718..5620)
                     /locus_tag="NBI42_002359"
     CDS             complement(4718..5620)
                     /locus_tag="NBI42_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCD1778750.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NRAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGMILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLINE"
BASE COUNT         2047 a          782 c         1037 g         1757 t
ORIGIN      
        1 acatgagtaa aactcatgca taagaaatac taatttctat agaaaaagta tttctttatc
       61 gttgtccccc caacttgcat tgtctgtaga atttcttttc gaaattctct gtgttggggc
      121 ccctgactag agttgaaaaa aagcttgttg caagcgcatt ttcactcagt caactactag
      181 caatataata ttatagaccc taggacattg atttatgtcc caagctcctt taaatgatgt
      241 atatttttag aaatttaatc tagacatagt taaaaataaa tataaaacat cgttgcttaa
      301 ttttgtcata gaacatttaa attaacatca tgaaattcgt tttggcggtg aaaaaataat
      361 ggataataat gaaaaagaaa aaagtaaaag tgaactatta gttgtcacag gtttatctgg
      421 cgcaggtaaa tctttggtta ttcaatgttt agaagatatg ggatattttt gtgtagataa
      481 tctaccacca gtgttattgc ctaaatttgt agagttgatg gaacaaggaa atccatcctt
      541 aagaaaagtg gcaattgcaa ttgatttaag aggtaaggaa ctatttaatt cattagttgc
      601 agtagtggat aaagtcaaaa gtgaaagtga cgttatcatt gatgttatgt ttttagaagc
      661 tagtactgaa aaattaattt caagatataa ggaaacgcgt cgtgcacatc ctttgatgga
      721 acaaggtaaa agatcgttaa tcaatgcaat taatgatgag cgagagcatt tgtctcaaat
      781 tagaagtata gctaattttg ttatagatac tacaaagtta tcacctaaag aattaaaaga
      841 acgcattcgt cgatactatg aagatgaaga gtttgaaact tttacaatta atgtcacaag
      901 tttcggtttt aaacatggga ttcagatgga tgcagattta gtatttgatg tacgattttt
      961 accaaatcca tattatgtag tagatttaag acctttaaca ggattagata aagacgttta
     1021 taattatgtt atgaaatgga aagagacaga gattttcttt gaaaaattaa ctgatttgtt
     1081 agattttatg atacccgggt ataaaaaaga agggaaatct caattagtaa ttgccatcgg
     1141 ttgtacgggt ggacaacata gatctgtagc attagcagaa cgactaggta attatctaaa
     1201 tgaagtgttt gaatataatg tttatgtgca tcatagggac gcacatattg aaagtggcga
     1261 gaaaaaatga gacaaataaa agttgtactt atcggtggtg gcactggctt atcagtcatg
     1321 gctaggggat taagagaatt cccaattgat attacagcga ttgtaacagt tgctgataat
     1381 ggtgggagta cagggaaaat cagagatgaa atggatatac cagcaccagg agacatcaga
     1441 aatgtgattg cagctttaag tgattctgag tcagttttaa gccaactttt tcagtatcgt
     1501 tttgaagaaa atcaaattag cggtcactcg ttaggtaatt tattaatcgc aggtatgact
     1561 aatattacga atgatttcgg acatgccatt aaagcattaa gtaaaatttt aaatattaaa
     1621 ggtagagtca ttccatctac aaatacaagt gtgcaattaa atgctgttat ggaagatgga
     1681 gaaattgttt ttggagaaac aaatattcct aaaaaacata aaaaaattga tcgtgtgttt
     1741 ttagaaccta acgatgtgca gccaatggaa gaagcaatcg atgctttaag agaagcagat
     1801 ttaatcgttc ttggaccagg gtcattatat acgagcgtta tttctaactt atgtgtcaat
     1861 ggtatttcag atgcgttaat tcattctgat gcgcctaagt tatatgtatc taatgtgatg
     1921 acgcaacctg gggaaacaga tggttatagc gtgaaagatc atatcgacgc gattcataga
     1981 caagctggac aatcgtttat tgattatgtc atttgtagta cacaaacttt caatgctcaa
     2041 gttttgaaaa aatatgaaga aaaacattct aaaccagttg aagttaataa ggctgaactt
     2101 gaaaaagaaa gcataaatgt aaaaacatca tctaatttag ttgaaatttc tgaaaatcat
     2161 ttagtaagac ataatactaa agtgttatcg acaatgattt atgacatagc tttagaatta
     2221 attagtacta ttcctttcgt accaagtgat aaacgtaaat aatatagaac gtaatcatat
     2281 tatgatatga taatagagct gtgtaaaaaa aatgaaaata gacagaggtt ctaaggtgaa
     2341 tcatgtttta aataagaaag gaatgactgt acgatgagct ttgcatcaga aatgaaaaat
     2401 gaattaacta gaatagacgt cgatgaaatg aatgcaaaag cagagctcag tgcactgatt
     2461 cgaatgaatg gtgcacttag tctttcaaat caacaatttg ttataaatgt tcaaacggaa
     2521 aatgcaacaa cggcaagacg tatttattcg ttgattaaac gtgtctttaa tgtggaagtt
     2581 gaaatattag tccgtaaaaa aatgaaactt aaaaaaaata atatttatat ttgtcgtaca
     2641 aagatgaaag cgaaagaaat tcttgatgaa ttaggaattt taaaagacgg catttttacg
     2701 catgaaattg atcattcaat gattcaagat gacgaaatga gacgcagtta cttgagagga
     2761 gcttttctgg caggtggctc agtgaataac cccgaaacat cttcgtacca tttggaaatt
     2821 ttttctcaaa atgagagtca tgcagaaggc ttaacgaaac taatgaatag ttatgagttg
     2881 aatgccaaac atttagagcg aaaaaaagga agtattacgt atttaaaaga agcggaaaag
     2941 atttcggatt ttcttagttt gataggtggc tatcaagcgt tattaaaatt tgaagacgta
     3001 cgtattgtaa gagatatgcg taattctgtt aaccgactcg ttaattgtga aacggcaaat
     3061 ctaaataaaa cagttagtgc tgcgatgaaa caagttgaga gcattaaatt gattgataaa
     3121 gaaattggta ttgaaaattt accagacagg ttgagagaga ttgctagaat tcgagtagaa
     3181 catcaagaaa tttcgttgaa agagcttgga gaaatggtat caactggtcc aatttcaaaa
     3241 tcaggtgtaa atcatcgatt aagaaaactt aatgatttag ccgacaagat tagaaatggt
     3301 gaacaaatag aattataagt aagaaggtgt ttttggtagt gaattatcaa aaataccttt
     3361 tttatgatta aaaagtgttt aatgaataat agtcgactaa ctattaaatt aagctggaaa
     3421 gtttaatgaa ggaaattcaa taaccaagcg ttacctaatt ttctcattaa gtagcatcat
     3481 atatatcaat ttggtcataa gaaaatacga atgtgataat taattgagtc cctgaaagtc
     3541 cctgatcata gacaaaaaaa tcagcataaa aagaatcccg acattgcggg attctctgta
     3601 ttgaaaagta ttgtatttta tttaaatgcc tccttgaagg gaatcgaacc cctatcttaa
     3661 gaaccggaat cttacgtgtt atccattaca ccacaaggag taaatatgtt aatcaaatca
     3721 ctgtactatc agaagtaaaa taagtacaat ctaaaataga tattatcagt ttacaaagga
     3781 aagagacaag cgtcaaacaa tgtaactatt taaagtcaaa gtgtttgacc aaatttgact
     3841 taatatgtaa aataatgagt aacagttatt acaaggagga aatatagatg aatttaattc
     3901 ctacagttat tgaaacaaca aatcgcggtg aacgtgcata cgatatatac tcacgtttat
     3961 taaaagaccg tattattatg ttaggttcac aaattgatga caacgtagca aattcaatcg
     4021 tatcacagtt attattctta caagcacaag actcagagaa agatatttat ttatacatta
     4081 attcaccagg tggaagtgta acagctggtt ttgcgattta tgatacaatt caacacatta
     4141 aacctgatgt tcaaacaatt tgtatcggta tggctgcatc aatgggatca ttcttattag
     4201 cagctggtgc aaaaggtaaa cgtttcgcat taccaaatgc agaagtaatg attcaccaac
     4261 cattaggtgg tgctcaagga caagcaactg aaatcgaaat tgctgcaaat cacattttaa
     4321 aaacacgtga aaaattaaac cgcattttat cagagcgtac tggtcaaagt attgaaaaaa
     4381 tacaaaaaga cacagatcgt gataacttct taactgcaga agaagctaaa gaatatggct
     4441 taattgatga agtgatggta cctgaaacaa aataattcaa agtaaagagt agactaagca
     4501 gtctgctctt tttgtatgag taaaccaagg tatcaataaa ttgtttacta tactttgagc
     4561 ggaaatatga ttgaatgaag ctagttgaac cgtaactata tgaaatgttc ccttcaaagt
     4621 agacattgaa aggaacattt caatcctttg tttgtaagtc gctctagaca ttatttttag
     4681 tatatatgtt gtttcgaatg cgcatcaatg gtattgatta ttcgttaatt aaatcttcaa
     4741 gagccatttt taaattacta tatttaaatt ggaatcctaa tgcttgaatt ttattaggta
     4801 atactttttg agtatccaat actactgttg acatttgacc aagtatgaga cgcattgcaa
     4861 gacttggtgc ccaagtttca tgaggcttat gcatagctct tgctaaagtg tagccaaata
     4921 aattttgacg ctcaggtata ggtgcagtta aattaaacgg accactagct gactcgttat
     4981 ttattaaaaa taaaatagct tgaattaaat cattgatatg aatccatgaa taccattgtt
     5041 gaccagaacc taatttacca ccaatgtaat attcgtatgg tagtttcatt gtttgtaacg
     5101 caccgccttc attcgataaa atcataccga aacgaccgat gacaactcgc gtacctaatt
     5161 gttcaaattg ttgtgcgaaa cgttcccatt gatacacaat atctgataag aaatcaaatg
     5221 gtaaagtttt ataaacttct gtgtaactca taaataaatc aggaggatag taaccagtgg
     5281 cactagcatt aaataaaact ttaggtgctc tattacgtga tttaaacaat tcatataaag
     5341 cttgcgtaga ttgaattcta cttagcatta gcgtttgttt atattccggt gtccatcgtt
     5401 tattcaatgt agcacctgct aagttgatga ccacatcgat attttgagga actttgtgtt
     5461 cccacccaga tttagcccag ttgacatatg aaattttctt atcatttgaa atttggtcgt
     5521 gtcgcgttaa tatcgtgata tgtgaatctg attttttgat ttcattaact aattgagatc
     5581 caaccatacc agttccacca gtaattaaat attgtttcat tat
//
LOCUS       DAHHRR010000143         5608 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.144, whole genome shotgun sequence.
ACCESSION   DAHHRR010000143 DAHHRR010000000
VERSION     DAHHRR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.144"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            181..954
                     /locus_tag="NBI42_002360"
     CDS             181..954
                     /locus_tag="NBI42_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1778751.1"
                     /translation="MIHSRKLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKDMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNRIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAEYQLSNNDYNVKQLRKRYDILTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFSFVTSKEENIYFTDSINFKPTE"
     gene            1198..1983
                     /locus_tag="NBI42_002361"
     CDS             1198..1983
                     /locus_tag="NBI42_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1778752.1"
                     /translation="MIHSKKLTLGICLVLLIILIGGYVIMTKENGRNAQIKEAFNKTL
                     KVYPTKNLDDFYDKEGFRDQEFDKRDKGTWIINSGMNIQFKGGALKSREMVLYINRNT
                     RTTKGYFIVGEITKDKKGYTHDKDKKYPVKMEHNQIIPTKPIKDEKLKKEIENFKFFV
                     QYGNFKDFKDYKNGDISYNPNVPSYSAKYQLSNDDYNVQQLRKRYDIPTKQAPELLLK
                     GDGDLKGSSIGSKDLEFNFVRNKEENVYFSDSVLFTPSEDDKL"
     gene            1980..3304
                     /locus_tag="NBI42_002362"
                     /pseudo
     CDS             1980..3304
                     /locus_tag="NBI42_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3304..3630
                     /locus_tag="NBI42_002363"
     CDS             3304..3630
                     /locus_tag="NBI42_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778753.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQLDELRHQTRRKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            3633..3920
                     /locus_tag="NBI42_002364"
     CDS             3633..3920
                     /locus_tag="NBI42_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778754.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
     gene            4237..4344
                     /locus_tag="NBI42_002365"
                     /pseudo
     CDS             4237..4344
                     /locus_tag="NBI42_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014533130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
     gene            4456..5250
                     /locus_tag="NBI42_002366"
     CDS             4456..5250
                     /locus_tag="NBI42_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1778755.1"
                     /translation="MIHSKKLKLCLCLIILSIFIGGCGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDEEFEKGDKGTWIIYSEMVIEPKGKNMETRGMVLYINRNT
                     RTTKGNFIVNEITDDNDGRPIDNKKKYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFV
                     QYGNFKDINDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLK
                     GDGDLKGSSIGSKNLEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(5242..5376)
                     /locus_tag="NBI42_002367"
     CDS             complement(5242..5376)
                     /locus_tag="NBI42_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778756.1"
                     /translation="MSYIIKFTFKFFEMTTYISQLTLDIELVSSELPLILSDFICYSV
                     "
BASE COUNT         2332 a          692 c          986 g         1598 t
ORIGIN      
        1 aggccaataa aaattataaa gatatttgcg ctttttaaat aatttgtgat gttgatatgt
       61 gaaaaataca actaacaatt aaaatcgacg agaatttaat cataaaattt cctctagtat
      121 tattaatatt ggaatttgaa cttaactttg catcaaaaat aaaaatgggg atgtgaaatg
      181 atgattcatt caagaaagtt gaggctttgg ctgtatttag tattattagc tgtttttata
      241 ggcgcttgcg gaatgaaaaa ggaagaaagc agtaaggata aacaaattaa agaaaacttc
      301 aacaaaacgt tgagcttgta tccaactaaa aatcttgaag acttttatga taaagaagga
      361 tttcgagatc aagagtttga aaagggcgac aaaggcactt ggataattca ctctaaaatg
      421 acaatagaaa caaatggaaa ggatatggaa tccagaggct tggtcctgta tgtagaccgt
      481 aataccagaa cgacaaaagg tgaatttatt gtgagagaat tatgggaaga taaaaaagga
      541 tactcacgta gtaaagaaaa agaatatcca gtaaaaatgg aacataatcg aattatccca
      601 actaaaccga tagctgatga taaattaaga aaagaaattg aaaacttcaa gttttttgta
      661 caatacggtg actttaaaga tattaatgat tataaagatg gtgacatatc atataatccc
      721 aatgtaccta gttattcggc cgagtatcaa ttgagtaaca atgactacaa tgttaagcaa
      781 cttagaaaga ggtatgatat actaactaag aaagcaccta aattattaat aaaaggcgat
      841 ggtgatttaa aaggatcatc tataggacac aaaaatttag aattttcttt tgtaacaagt
      901 aaagaagaaa acatctattt tacggatagt attaatttca aacctactga atagggttaa
      961 tagaagtttg aaatgaaaat ttcatacagt actaggtaaa aaattataac gataactctt
     1021 aaaattaata ataaatcaca gaaactttat cttttgattt aattgtttta taattctgat
     1081 aagaatattt aaaatacaaa ataattacaa ttaaattcat ttgttcaaaa caatttttcg
     1141 ttttagttgt aaatattaaa aactgagtca ctattaaaaa tataaggtgg agaaatgatg
     1201 attcattcaa aaaaattaac gctcggtata tgtttggtat tactcattat attgattgga
     1261 ggttatgtca ttatgacaaa agaaaatggt cgaaacgctc aaattaaaga agcatttaat
     1321 aaaacattaa aagtatatcc aaccaaaaat ctcgatgatt tttacgataa ggaaggcttt
     1381 cgagatcaag aatttgataa aagagataaa gggacttgga ttattaattc tggaatgaac
     1441 atccaattta aaggaggagc cttaaaatcc agagagatgg tactttacat caatcgtaac
     1501 actagaacaa ctaagggata ttttatagta ggtgagataa ctaaagataa aaagggatat
     1561 acacatgata aagataaaaa atatcctgtc aagatggaac ataatcaaat tatcccaaca
     1621 aaacctataa aagacgagaa gttaaaaaaa gaaattgaaa acttcaagtt ttttgtacaa
     1681 tatggtaact ttaaagattt taaagattat aaaaatgggg atatttcata caatccaaac
     1741 gtaccaagtt attcggcaaa atatcaatta agtaatgatg attataatgt ccaacaatta
     1801 agaaaaagat atgatatacc aacgaaacaa gcaccagaac tattgttgaa aggtgatggc
     1861 gatttaaaag gttcatccat aggctctaaa gacttagaat ttaactttgt aagaaataaa
     1921 gaggaaaacg tctatttttc agattctgtt ctattcactc caagtgagga tgataaatta
     1981 tgagcctacc agaatataaa ataaaatctg acaatatcaa aggtaactct gaagaaacaa
     2041 gtgcagtatc taaaatcagt tatgagatag aaaacgcaaa taataatgga ttgaatgacg
     2101 aaagcattaa aaggcaaata aaaaagcgca aaataataat cgatttcctg aaaacttgtc
     2161 ttatgtaaga agctatacag atccaaaaac aggtacgact acaagcgcct ttttaaataa
     2221 agacactggc aaagttactt taggtatgac aggtactaat gtacacaaag acgcaatatt
     2281 aaaacaaaca tttggtgttc cttcttatca aggatatata gatgcgagtg aaacgctaaa
     2341 ggatattggg gccgatgtca atattggcct tcattccgtc acagataaag atccacatta
     2401 taaaaatacc caagacttta tcaaaaatat caaaaaagac tatgatattg atattattac
     2461 cggacattcg ctgggcggta gagatgcgat gattttaggt atgagtaatg atattaaaca
     2521 tatcgttgtg tataatccag ctccattagc gatcaaagat gtgagcggcc tttatgccga
     2581 tcaagaagaa cttaaaaaat tgattgaaaa gtacgatggt cacattgtaa gatttgtgtc
     2641 tgatgaagac gaattagatg caggtgtccg caatcattta tatgaaactg ctggagaaaa
     2701 aatagtactt aaaaatggag aaggccatgc aatgagtggt attttaatga gcagaacaca
     2761 ggctataatc ttagctgaat taaacaaagt taaaggctac caagacgaaa ataataaagc
     2821 attaaaatcc gttcgtaaac aaacgaggca tagattacat aaagtagaga cgttaagagc
     2881 gaattggatt caaataacag gtggacccct ctcttcctcc caacaacaat tattagaagc
     2941 tttaacagca ctaaccattg ccgaaggatt aaatcaatta gtgaatgaag aaagccaaca
     3001 tttgaaaaaa atgtatcatg tgatggcgca taaatttgga gacaactgga aaaaagcgca
     3061 aaaagttgga aatgaaattg gtgaaaaatt gacctctgca gaggttatag atgaattaag
     3121 aaaaggtggc gcgtatgaaa gtaaacttga aacagatcct aaaagaaaaa ttgatgataa
     3181 gataaagaaa ttaaatgatg tttataaaaa ttgtaatggc tatatcgcaa aaattaaaca
     3241 gagtatcgaa gcaattgttt ctaatgacca aatgttagcg agccagattg atgggatgat
     3301 gtaatgttta ctacgtataa aaatattaat gaacttgaaa atgcctatga tgaagaaaga
     3361 aagcaattga atgatgcatt caatcaactt gatgaattaa gacatcaaac acgcaggaaa
     3421 tgtgaacaaa tgtatgatca tttcttatat ctcaaacata aaatgaatta tagtgaagac
     3481 gctatgatca ggatgacacg tattatagaa tctttcgata gagaaacgaa tcaacgtatc
     3541 cgacatcacg aaatgaaatt agaagattat aaagatgagt taagaagaga atatctaaaa
     3601 caatctgaca gaattgaagg agatgaataa gcgtgggact tgattttagt ggtttaccag
     3661 atttagcagt attggaacaa atgaaggaaa aagaacagat tagtgaggtt attgcgcctg
     3721 aacatgttcg tatgcatcat gatcatcaaa ataagctgaa aagtgatgag aaaatattac
     3781 ttgaccaaat ggtaagtcat ttcaaaaaat ttgaagatga ttttaaaaat gcggcacaag
     3841 gggcttgggt gaaaaatgcc acagacgaat taaaagatat tagtaatgat ttagaaaaaa
     3901 ttcaagatat taaagtgtaa aaaggtatta agaaacacaa taagtatata atctatttaa
     3961 taataaatga attatatagt tcgtaatttc gactataagt ggctattagc cattattttt
     4021 cgggatctat gtcaaatcgg actaatgaat tcaataatgg aagttaagca accaatcttt
     4081 gtttaacttc ttttttattt tttggaaaat aaagttttga acataataaa atttgattat
     4141 gttttaacga attttgatgt ttcttgaact atatatcatc tagtcgtcat ttacaattgg
     4201 taaatatgac ttcaaactgt atgaaggtga tggcgattaa aaggctcatc cgtaggttct
     4261 aaagaactag aatttaactt tataagaaat aaagaagaaa atgtgtatta ttctgattgt
     4321 attaatttca aacctactga atagagttaa tagaagtttg aaatgaaaat tccataaagt
     4381 gttcaaaata atttttcgct ttagatatat aaattaaaga ttgagtcagt attaaaaata
     4441 taaggtggag aaatgatgat tcattcaaaa aaattaaaat tgtgtctatg cttgataata
     4501 ctatctattt ttataggggg ttgcggaatg aaaaaagaag aaagtagtaa agataaacaa
     4561 attaaagaaa atttcaacaa aacattgagt ttatatccaa ccaaaaatct tgaagacttt
     4621 tatgataaag aaggctttcg tgacgaagag tttgaaaaag gagataaagg tacttggatc
     4681 atttattctg aaatggttat cgagccaaaa gggaaaaata tggaaacgag aggaatggtg
     4741 ctttatatca atcgcaatac taggacaaca aaaggtaatt ttattgtcaa tgaaattaca
     4801 gatgataacg atggtagacc cattgataat aaaaagaaat accctgtaaa aatggaacat
     4861 aataaaatca taccaacgaa gccactaccg aatgacaagt taaaaaaaga aatcgaaaac
     4921 tttaaattct ttgtacaata tggcaacttt aaagatatta atgattataa agatggtgat
     4981 atttcatata atcctaatgt accaagttat tcggcaaaat atcaattgaa taatgatgat
     5041 tataatgtcc aacagttaag aaaaagatat gatattccaa ccaaacaagc gccgaaacta
     5101 ttattgaaag gcgatggaga tttaaaaggt tcatcaatag gatcaaaaaa tctagaattt
     5161 acctttgtag aaaataaaga agaaaacatt tattttacag attctgtaca atatactcca
     5221 agtgaggata caagatatga gtcaaactga atagcaaata aaatctgaca atatcaaagg
     5281 taactctgaa gaaacaagtt cgatatctaa agtgagctgt gaaatatagg tagtcatttc
     5341 aaaaaattta aaggtgaatt tgataatgta gctcaagggg attgggttaa aaaggcgaag
     5401 aatgaactgg atgatattag taagaaatta aaaaatattc aaagaacgga agtttaatag
     5461 cttatatgat tcctgaagct aagacagcat gcgttcattc ataccatcat taatataatc
     5521 accacaacaa aaaagcttct aacatgatat cggaatctcg gattccatat catgttagaa
     5581 gcttttaatg tctaaagaac atctacat
//
LOCUS       DAHHRR010000144         5594 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.145, whole genome shotgun sequence.
ACCESSION   DAHHRR010000144 DAHHRR010000000
VERSION     DAHHRR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.145"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            238..526
                     /locus_tag="NBI42_002368"
                     /pseudo
     CDS             238..526
                     /locus_tag="NBI42_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
     gene            complement(919..1482)
                     /locus_tag="NBI42_002369"
     CDS             complement(919..1482)
                     /locus_tag="NBI42_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCD1778757.1"
                     /translation="MNTKLLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKADS
                     LSDGQLWLGVKNPLIDAQIISGNKDEEIKVTYLRNDGVSNVVSAYGNNDGGNFTESID
                     FKSLKQIDLTKENVQDDFNKELDAKNTWNSLTEKLKSHGLVKNGQKLSIHSSNNPTPV
                     SGTVGEEVSTGGENKLTKRLINKITID"
     gene            2436..3139
                     /locus_tag="NBI42_002370"
                     /pseudo
     CDS             2436..3139
                     /locus_tag="NBI42_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
     gene            3264..3986
                     /locus_tag="NBI42_002371"
     CDS             3264..3986
                     /locus_tag="NBI42_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCD1778758.1"
                     /translation="MNKNVVIKSIAALTILTSVTGLGTTLVDNVQQTAKAENNVTKVK
                     DTNIFPYTGVVALSSATGFVVGKNTILTNKHVSKYYKVGDRITAHPNSDKGNGGIYTI
                     KKITDYPGKEDVSVIHVEERAVERGPKGFNFNDNVTPFKYAAGAKIGERIKVIGYPEP
                     YKNKYILHESTGPVASINGSSIVYSAHVEVGNSGSPVLNSNNELVGIHYASDANNGNN
                     KNGYGVYFTPEIKKFIAENIDK"
     gene            4044..4763
                     /locus_tag="NBI42_002372"
     CDS             4044..4763
                     /locus_tag="NBI42_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCD1778759.1"
                     /translation="MNKNLVIKSIAALTILTSMTGIGDSVREDKQQIAKAEKNVTQVK
                     DTNVSPYKGVVAFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KKISDYPGNEDISVMNVEEKAVERGANGYNFNENVEAFKFAKDAKVDDKIKVIGYPLP
                     AQNTFKQFESTGTVKSIKDNNLNFDAYIEPGNSGSPVLNSNNEVVGVVYGGIGKIGSE
                     YNGAVYFTPQIKEFIQKHIEQ"
     gene            4886..>5594
                     /locus_tag="NBI42_002373"
     CDS             4886..>5594
                     /locus_tag="NBI42_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCD1778760.1"
                     /translation="MNKNIIIKSIAALTILTSVTSVGTTMVEGIQQTAKAEHNVTKIT
                     NTSISPYKGILRVGAGTGFVVGKNTILTNRHVAKDVQVGSTVLAHPNGENDTGGYYKV
                     KKVIPYAGSADLAIVQVEEDSVYPKNKKFSENTEILTLTSEVKANERIAIVGYPAPYK
                     NKHHMYQSTGTVLSINGDKLVSDAFAEGGNSGSPVFNNKNEVVAVLYSGDQIGHSKKE
                     SYSVYFTPEIKKFIADNT"
BASE COUNT         2131 a          776 c          891 g         1796 t
ORIGIN      
        1 tacttagatt tagtaaataa tttaaatctt catcgttcag tttcaaatgc atttaaacat
       61 ctccttagtt tagattttac gatcttaaat ccttatatta ttttcagtat ttgtagtaag
      121 ttatacaggt gttttctata aacttaataa ttattccttt ttagcgtcat aatcgtttag
      181 caatacatga caatggaata tttattaatt tatttagtac cgtcattttt aacattatta
      241 ataatttgtg ataatgttag atatattttt cattaaagat gtaatggcta ttgttgccgg
      301 aatcttaatg tttattttta acgtttattt tggattagaa gtgcttggag atccttcgtg
      361 gttctattta ccgatcacat atgctacgcg ttatgtatat atgtatattg aaggaaatat
      421 accagtgatg atgacaaagg taatctatat tattatcacg ttgttattgg caattttact
      481 tttcaaagga ttcaataaat ggagtagcag aactattaaa gattaatctt agtgctgaaa
      541 atgataaata aaaatttgta tgagttactt tataattaag tagccaaata agagaaaaga
      601 gaagaggaac ataaattatt tccctttacg tttaaataca attgtgtttt atcgattaga
      661 cattttattg aattgtatgt tattatatga atataaataa gacgacatgc gcgaacatgt
      721 cgtcttagta agccaaacgc tggcttctaa aaatattttt ttaaccattc caacttgcca
      781 aagttaatgc ggaatggttt tttattttat ccgctatttg aaataatcaa tgacgttata
      841 atatattatg ggctaggtgg tttgtaaaaa agggttagtt attaaggtta gtaaataaat
      901 caatttaagt taaatttctt agtcaattgt aattttattg attaaacgtt tagttagttt
      961 attttcacca ccggttgata cttcttctcc cactgtgcct gaaactggtg taggattatt
     1021 cgaagaatga atagatagtt tttgtccgtt ctttactaaa ccatgacttt ttagcttttc
     1081 ggttaaagaa ttccatgtgt ttttagcgtc tagctctttg ttgaagtcat cttgaacatt
     1141 ttctttagtt aaatcaattt gttttaggct tttaaagtca attgattctg taaaattacc
     1201 tccatcgtta tttccatatg ctgatactac atttgaaact ccatcatttc ttaaataagt
     1261 tacctttatt tcctcatctt tatttccgct aataatttgt gcatcaatta aaggattttt
     1321 aacgcctagc cataattggc catcagataa actatctgct ttagctgttt taacaacttt
     1381 atttgtatct aatcctaagt atgaagaatg taagcctgtt cctaatgttg ttaatactaa
     1441 agcacttgct actaatgttt ttcctaataa ttttgtattc attttaattg ctcctttttt
     1501 tatattgtaa acgtttacaa tgcaaatata ataatatttt tttaaaagaa caattaacta
     1561 aatatcaaaa atgtattaac tatctattaa ctaaaaaata gaataatttt ttaacatcgt
     1621 tttgttgttt tgaattataa aaattaaagt aaaaatgttg ggaatgaaag attgttggtg
     1681 agaattttga gatgtagata aatatcaaac tagtctatag gtaaaaattt ataattaatt
     1741 tgaaaagaat gtctcaaata atgtttgaat cagcaggtgt catattaagt aaacatttta
     1801 tactttatca gaacaaaaaa gacatgcatc aacatgtcag tgaaactaat caatattatc
     1861 ttaacaacga aattgaatca aatctgaaaa acgtatttac aaatctcaac tactctatcc
     1921 aagtcaaaat cgcaaaatct aatccctgaa ataacatatc tcgaaaaaaa ttccataatt
     1981 caatcaactt caataactaa caatacatta ttagttccaa atatactcat acatgtttca
     2041 ttgattgact gcatattgtc ataaataata ggggaactaa ttgctaataa tattgttaaa
     2101 cacaatacat aataaagtga aagactataa acacatgaat tgttcaccaa tccgccataa
     2161 attttcaatc acctaatttc cgaataaata ttacaatttc agttctataa ttaatattta
     2221 agaccaaaat aatcctgaaa agtcaatttt aattgcgtga tattgagttt taatcatatt
     2281 taaataaatg agtcaactaa tgtataagga tttaataaaa agttgataag tagttaatta
     2341 atatttaatt aatattttta tataaactgt atatagtaaa tgtttatata gatacattaa
     2401 ctataaaaat aaatttaaaa ggaggtaaaa tgtgaattga aaatgtaata atcaaatgtc
     2461 taatagtttt tactatttta acatctcttg gtttcgctgg aaatatttct gatcagcctc
     2521 attcaatcgc caaagcagaa aagaatgtca aagaaattac cgatgcaact aaggcaccat
     2581 acaattcagt ggtagcattt gcgagtggta ctggtgacgt tgttggtaaa ctacaatctt
     2641 aaacaacaaa catatcgcta aaagtaatga tatttttaaa aatagagtat cagcacatca
     2701 ttcgagtaaa ggtaaaggcg aaggaaacta cgacgttaaa gatattgtag aatatcccgg
     2761 aaaagaagac cttgcgatag atcatgttca tgaaacaagt acagaaggtt tgaattttaa
     2821 taagaacgtt agttatacaa aacttgaaga cggagcaaaa gcgaaagata gaatctctgt
     2881 tattggttat ccaaagggtg cacaaacaaa atataaaatg tttgaatcaa ctggaacgat
     2941 taatcatatc aatggaacgt ttatagaatt tgatgcgtat gcgcaaccag gaaactctgg
     3001 atcacctgta ttgaattcta aacatgaact gattggtatt ttatatgcag gtagtggaaa
     3061 agatgaatct gaaaagaatt tcggtgtcta tttcacacca caattaaaag aatttattca
     3121 aaacaatatt gaaaaataag tatcatcaat tcattcgtga agttgatttt ttaaagagca
     3181 attaagaaaa cggttacata actaactgaa tatactaaat tatgtatcta gatatcaaaa
     3241 taattaaaaa agaggaactt aaaatgaata aaaacgtagt catcaaaagt atagcagctt
     3301 tgacaatatt gacttcagtt acaggattag gaacaacgtt agttgacaat gttcaacaaa
     3361 cagctaaagc agaaaataat gtcacaaaag taaaagatac aaatattttt ccatatacag
     3421 gtgtagttgc cttgagtagt gcaaccggat ttgttgttgg caaaaacaca atattaacta
     3481 ataaacatgt atcgaaatat tacaaagtgg gcgaccgtat tactgcacat cctaatagtg
     3541 acaaaggtaa tggcggtata tatacaatta aaaaaattac tgattatcca ggtaaagaag
     3601 atgtttcagt cattcacgtt gaagaacgtg cagttgaacg tggaccaaaa ggatttaatt
     3661 ttaacgacaa tgtaacgcct ttcaaatatg cggcaggtgc taaaattgga gagcgtatta
     3721 aagtaattgg ttacccagaa ccatataaaa ataaatatat tttacatgag tcaacaggcc
     3781 ctgtggcgtc cataaatgga agtagcatag tatattcagc tcatgtcgaa gttggaaact
     3841 ctggatcacc tgtattaaac agcaacaatg aacttgtagg aattcactat gcttcagatg
     3901 cgaataatgg taacaacaag aatggatatg gcgtttattt cacaccagaa attaaaaaat
     3961 tcattgcaga aaacatagat aaataaaatt attagcttca aacgagcatt aaaatatatc
     4021 tcgaattgta aaggagcttg aatatgaata aaaacttagt tatcaaaagt atagcagctt
     4081 tgaccatatt aacatctatg actggcatag gtgattcagt ccgagaagat aaacaacaaa
     4141 tagcgaaagc tgagaaaaat gttacacaag ttaaagatac caatgtctct ccatataaag
     4201 gtgttgtggc atttaaagat gcgacaggtt ttgtgattgg taaaaatacg attatcacta
     4261 ataaacatgt atcgaaagat tacaaagtgg gtgatcgaat taccgcacac ccaaatggcg
     4321 acaaaggcaa cggtggcatt tataaaatta aaaagatttc tgattatcca ggtaatgagg
     4381 atatatcagt aatgaacgta gaagaaaaag ctgttgaacg tggagcaaat ggttataatt
     4441 ttaacgaaaa tgtagaagct tttaaatttg ccaaagatgc aaaagttgac gataaaatta
     4501 aagttatcgg ttacccttta ccagctcaaa atacatttaa acaatttgaa tcaactggaa
     4561 ctgtaaaaag tattaaagat aataatttaa attttgatgc atatatcgag cctgggaatt
     4621 caggatcacc agttctaaat tctaacaatg aggtcgtagg agtagtgtat ggcggtattg
     4681 gaaaaatcgg atcagaatac aatggtgcag tttattttac gcctcaaatc aaagaattta
     4741 ttcaaaagca cattgaacaa taaacatatt taaatttaca ccaagaacat gttgttcaat
     4801 gattttaatg aaaaaacatc ggtgaatata acataaaaaa acaaatgtct ataacaaaac
     4861 atcatgaaaa acagaggagc aaaaaatgaa taaaaatata atcatcaaaa gtatagcagc
     4921 attgacgatt ttaacgtctg tgactagcgt cggcacaaca atggttgaag gtattcaaca
     4981 aacagccaaa gccgaacata atgtgacgaa gataacaaat actagtattt cgccatacaa
     5041 aggcatacta agagtaggtg caggtacagg atttgtcgta ggtaaaaata ccattttaac
     5101 caatagacat gtcgcgaaag atgttcaagt aggcagtacg gttcttgccc acccgaatgg
     5161 cgaaaatgat acaggcggat actataaagt caaaaaagtt atcccatatg caggctcggc
     5221 agatttagct attgttcaag ttgaagaaga ttcagtttat cccaaaaata aaaaattcag
     5281 tgaaaatact gaaatcctaa cattgacatc agaagttaaa gcgaatgaaa gaattgctat
     5341 tgttggctat ccagccccat ataaaaataa acatcatatg tatcaatcaa caggaacagt
     5401 actatcaatt aatggagata aattagtatc agatgcattt gctgaaggtg gaaattcagg
     5461 ttcaccagta ttcaataata aaaatgaagt tgttgctgtg ttgtatagtg gtgaccaaat
     5521 tggtcattct aaaaaagaat catatagcgt ctatttcaca ccagaaatta agaaattcat
     5581 cgcagataac acag
//
LOCUS       DAHHRR010000145         5593 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.146, whole genome shotgun sequence.
ACCESSION   DAHHRR010000145 DAHHRR010000000
VERSION     DAHHRR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.146"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..877)
                     /locus_tag="NBI42_002374"
     CDS             complement(<1..877)
                     /locus_tag="NBI42_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1778761.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTNVDSKGIIHIFHGMAEHMKRY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYPLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPTEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNIKVLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWE"
     gene            complement(1027..2700)
                     /locus_tag="NBI42_002375"
     CDS             complement(1027..2700)
                     /locus_tag="NBI42_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HCD1778762.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDSIIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(2858..4354)
                     /gene="glpK"
                     /locus_tag="NBI42_002376"
     CDS             complement(2858..4354)
                     /gene="glpK"
                     /locus_tag="NBI42_002376"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCD1778763.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKP
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAYLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDENEREKLYRGWKKAVEATQVFKTE"
     gene            complement(4484..5302)
                     /locus_tag="NBI42_002377"
     CDS             complement(4484..5302)
                     /locus_tag="NBI42_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCD1778764.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVYAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
BASE COUNT         1721 a         1145 c          761 g         1966 t
ORIGIN      
        1 cttcccaaat tttgtcatga tctttttcaa ataacacttc atgacgttta tgatgataaa
       61 gattaacttt aacattttta atgccggctt ttctatatat attcgccaat tttaaaatcc
      121 ctttaccata atcacctaaa ggatcgtcat atcctgaaac taataatata ggcatggcat
      181 gatttaacac ttttatattt tttaattgtg atgtatgtaa catataatag gctgtttgat
      241 ataataattg atttgacaca ttaaatccac tatatggatc tttaataaag ttgtctactt
      301 cagttggatt actagaaatc caatcacttt gtgtacgtaa tggacgtatt tttttattaa
      361 aactatttga tactaactgg ttaacccatt caacccgttt ctcagcacca taaatttttg
      421 taatcagttg taacacctta acggttggta atcctttcca taatggatac ataccagtac
      481 cacttaaaat tagaccatca acatattgcg gatatgtttc tacaaacaat ctagctataa
      541 cggatcccat tgaatgtccg attataatgt atggtttatc aacattgcct ctcactgttt
      601 gcgctacttc aaaggcatca ccgataactc gtttcatatc atcgtaatgc cctcttgttg
      661 attcatcaat attaatacca tgtcctcgat gattatgacg tatcacatcg aagccatgct
      721 ttgaaagtgc atgtgctaat ttatcgtaac gtttcatatg ttcagccata ccatgaaata
      781 tatgaataat tccttttgaa tctacatttg taaaattcac tttaacttca atattcgtat
      841 tatcttgtac agttaattta aattgttttt cagtcatcat ctcacacctt taataacaac
      901 gtttttatat tcgcttttat tattatcata catttagtga tgcatagttt caattgacta
      961 atgtatctca tataaaaaaa ctgatgcccc taaaacagga caccagttta tgattgtaca
     1021 aattaattat tctttaactg ctggttgatt atttccatgt tgcgcttctt taattgcttg
     1081 ctcaacttct tcagtatatg cctcaatttg agctggtgag tagtctaaca tatcagccat
     1141 aatatcgatg atactatctt tataatctaa tacatcttta atattgaagt acattttacc
     1201 agaacgacga actaagaaat cgttaggttt gtataccatt tcttgttgaa tactataaac
     1261 aagttctaca taaatttcta atggtaactt gctatcatgg tattgagatg tttgcgcaat
     1321 gttgaacaat tcatcaacat ttgaaccata tttagatgct aaacgtcttg caacatcttc
     1381 atcaatgccg aatcctttag ctacatctac tttttgctct acaaacgcat caaagttctt
     1441 gctacctcct acatcgccac ctgaaattgc cagacctttt gtattacatg gactaaatgt
     1501 taaaccgtag tcttttttca agcgtttaga tactaaatca acaatgtctt gagccatgtg
     1561 acgatagcct gttaatttac cacctgcaat agttaataaa cctgatttac cttcccaaat
     1621 ttcatcctta cgagagattt cagaagggtc tttgccttct tcgtaaatta atggtctaat
     1681 tcctgcccat gttgattcaa tatcttcatc tgtaacatta acactaggga acatgtaatt
     1741 aatagcatcg attaaatagt ctctgtcttc ttgtgtagtt aatggtgaag atttgatatt
     1801 gtcatagaat gtatctgtag tacctacata cgcttttcct tcacgcggga ttgcaaaaat
     1861 cattcttcca tctttttcag tatcaaagta tactgcttga cctaatggga attttgattg
     1921 atcaataaca acatgtacac ctttagttaa acgtaatttt ttattattgc gtgcataatc
     1981 accacttcta acatcatcaa cccatggacc tgctgcatta accacttttt tagccttaat
     2041 tgtataattt tcattagtta atttatcaat aactttaaca ccatttactt gttgattttt
     2101 atcataagtg aagtgttctg acttagtata gttgataatt tctgcgcctt tttcagcagc
     2161 acgcttcata acttcaatag ttaaacgcgc atcgtcagta cgatattcaa catagtaacc
     2221 gccgcctttt agaccttctt ttttaactaa tggttcttta gctaaagttt cttttttaga
     2281 taacattttt ttacgttcag actttttaac acctgctaaa cgatcataca ttcctaaacc
     2341 aattgatgtt gagaatttac caaatgttcc acctttatgc attggtaaaa gcatccactc
     2401 tggagtcgta acatgaggcc cattttcata aacaatcgca cgttctttac cagtttcggc
     2461 aactactcca atttggaatt gttttaagta acgtaaacca ccatggacta attttgtaga
     2521 tcttgagctt gttccttgtg caaagtcttg catttcaact aatgcaactt tcattcctct
     2581 ttcactcgcg tctagtgcaa tacctgcacc tgtaataccg ccaccaataa tcactaaatc
     2641 atattcatca tttcttaaat tctttttaat atgttctctc ttaaaagtag acaatgccat
     2701 tccaaaacgc ctcctaaaaa atgtaaaaac gagagacttg tcccacataa acaatccttc
     2761 aattgtacat gttcgaacaa atctctcgtt tctcgattca aatattaact taaattgatt
     2821 gtatcacaat gtttatacaa aagtctaatc tacaagttta ttctgtttta aaaacttgtg
     2881 ttgcttcaac agcttttttc caacctctat ataatttttc tctttcgttt tcatccattt
     2941 tcggatcgaa tttttcttct aatttccagt ttttagcgat atcatcttta ctctcccaga
     3001 atccaactgc taaacctgct aaatatgcag cacctaaagc tgtagtttct tgaatttcag
     3061 gtctttcaac agaagtatta acaatgtctg cttggaactg cataataaag ttattcttaa
     3121 ctgcaccacc atcgacacgt aaactttgga catcgatacc agagtctttt gacattgctt
     3181 ccataacgtc acgagtttgg taacataatg attctaatgt tgcacggata aagtgctctt
     3241 tttcagttcc acgtgttaaa ccgaaaatcg caccacgtgc ttcagaatcc caatatggtg
     3301 ttcctaaacc tacaaaagct ggaacaacat aaacaccctc agtagagtca actcgtgtcg
     3361 cataactttc tgattgtggt gctgaattaa tcattcttaa tccatcacgt aaccattgga
     3421 ttgctgaacc cgaaacaaag attgaacctt caagcgcata atttactttt ccatcaatac
     3481 cataagcaat tgttgttaat aaaccacttt cagatttaac cgctttgtca cctgtattca
     3541 ttaacatgaa gccaccagtt ccatatgtgt ttttcacgtc accacgttcg aagcaagctt
     3601 gtccaaataa tgctgcttgt tgatcaccag ctacaccagc aattggtact tcttgaccat
     3661 agaagtggta atcaattgtc ttaccatata cttcacttga aggtttaact tctggcaaca
     3721 tatttttagg tactgtaagt agttctaata actcatcgtc ccattctaaa tcatggatat
     3781 taaacattaa tgtacgactc gcatttgaat aatcagtaat atgcgcagct tttcctgata
     3841 atttccatac taaccaagta tcaatcgttc caaataatag atcgccattt tctgcttttt
     3901 ctcgtgcacc ttcaacattg tctagaatcc atttaacttt tgtacctgca aaatacggat
     3961 ctaaaagtaa tcctgtctta tctctaaatg tttgttcata tccttgttgt tttaattctg
     4021 aacaaattga ttgtgtttga cgtgattgcc aaacaattgc gtgataaatt gggcggccag
     4081 tatgtttgtc ccaaacaacc gttgtttcac gttggtttgt aataccgata cctgcaattt
     4141 gatcagctct aacatcattt tcattaatta cttccgtcat tacagctaac acagatgtcc
     4201 aaatttcatt tgcatcatgt tcaacccaac ctgattgtgg aaaatattgc ttaaactcac
     4261 gttgtgctac ccctgcaatt tccccttttt gattgaacaa aatcgctctt gagcttgttg
     4321 ttccttggtc tatagataaa atatattttt ccattagtag tatctccttt attaaaaaaa
     4381 taatcgctaa cccattgtac aaaatctgtt aaattttggc gaaaaaatag cataatccat
     4441 gaatcaaaca tgttaaatta tgctatttac gtttcttttt attttagtaa attgattcga
     4501 tatcaccttt ttttgatgat ttatttaaaa tgtaacctaa aatcaaagta ataataacta
     4561 caacaattgc aattgcacaa ccaatattaa atgtatgttt ataaaataca gcgtatacta
     4621 ccgcacctaa taaaccaccg gcaattggtc ctaagatagg aacgattgca tatgaccaat
     4681 ttgaaccacc tttaccagct attggtaaaa tcgcatgtgc aattctcgga cctaaatcac
     4741 gtgctgggtt gattgcataa ccagtagcac cgcctaaact taatccgatt gcaacaatta
     4801 atgctccgac aattaaagga tttaaaccat cggcaatttt gtttacaccg ataaataaaa
     4861 tacctaaagt taatgccatt gttccgataa tctcacttaa aaagttggca aagtaattct
     4921 taatagccgg tgctgtagag aaaacaccta atttcgcgcc agcttcttct gtcgctttcc
     4981 aatgtggcaa gtacattaac catacaatcg ttgctccgac aattgcacct aacatttggg
     5041 caacaatata accaggaact aatgaccaat caaaacttcc gtctaatgca agagctaaag
     5101 acaccgctgg gtttaaatgt gcacctgaga attgaccgac agcatacaca cccattgtaa
     5161 ccgctaatcc ccatccagct gtgatgacaa tccaatcagc accatttgct gcacttctct
     5221 ttaaattgac attggcacat acgcctccac caaaaaggat taagattgca gttccaagga
     5281 attctgctaa atatacattc atattaaatg tcctcccaaa agacaaagag attcctacac
     5341 tagcagtttg gctcgtagaa atctcccgtc tctattctat gtattaactt ttattatatt
     5401 tgttataata ctatcgttat gaaagcgttg tcaatggttt ttgtaaaaat tttgtcaaat
     5461 ttaatttttc aagtctttat aaaagtagtt taatttgaaa actagaaata tcaatcctca
     5521 ctaaatagtc tatgtcgttc agttgcttaa atgacattct tcagccatat tttttatgac
     5581 tagttgcatt ttt
//
LOCUS       DAHHRR010000146         5367 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.147, whole genome shotgun sequence.
ACCESSION   DAHHRR010000146 DAHHRR010000000
VERSION     DAHHRR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.147"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..217)
                     /locus_tag="NBI42_002378"
     CDS             complement(<1..217)
                     /locus_tag="NBI42_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCD1778765.1"
                     /translation="MKISRLISIILILNERKRVSAHELSELMEVSQRTIYRDIEAINI
                     AGIPVYSIPGVGGGFEIMENFKIDKNTF"
     gene            309..944
                     /locus_tag="NBI42_002379"
     CDS             309..944
                     /locus_tag="NBI42_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HCD1778766.1"
                     /translation="MKNVYFYIIDGLADWEISNILAELNSKRFFKKDAQTINIEMVSN
                     SKTSITTMGGINIKPECLIEEISISKDTFLILPGSDTWNDSKHLPVINIAKEILSNGG
                     YVAAICGATIPIADIGILNEHYHTSNDLLYLKSFSSNYHGQKLYIDQPSVSYKNLITA
                     SSTGALMWTKDILHHLDVFKHSTLDAWFNYFSTGNSKYYFELQQTLNKDDN"
     gene            1133..1558
                     /locus_tag="NBI42_002380"
     CDS             1133..1558
                     /locus_tag="NBI42_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD1778767.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTMDGY
                     HTVPYTISVDGITALHRTYFIFSENKNVLYQEIDSKVKNELASQRGVTTEKIKNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            complement(1927..2631)
                     /gene="budA"
                     /locus_tag="NBI42_002381"
     CDS             complement(1927..2631)
                     /gene="budA"
                     /locus_tag="NBI42_002381"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCD1778768.1"
                     /translation="MANVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDEDFIN
                     ANIDYKDIADEIREAE"
     gene            complement(2668..4332)
                     /gene="alsS"
                     /locus_tag="NBI42_002382"
     CDS             complement(2668..4332)
                     /gene="alsS"
                     /locus_tag="NBI42_002382"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCD1778769.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEKDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELDTQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISDKVDEP
                     FINQQHLDELEQLRAHIDQETGIKATHEEGILHPVEIIESMQKVLTDETTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            complement(4794..4976)
                     /locus_tag="NBI42_002383"
     CDS             complement(4794..4976)
                     /locus_tag="NBI42_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778770.1"
                     /translation="MKDSIFWKKAFIPVYFIIAMLVFLLFRFYIKTDNFSIYLMIIFL
                     ICLGTASIIYNYKNNR"
BASE COUNT         1794 a          933 c          765 g         1875 t
ORIGIN      
        1 taaatgtatt tttatctatt ttaaaatttt ccataatttc aaagccacca ccgacacctg
       61 gaatagaata aacaggtatt ccagctatat ttatcgcttc tatatctcga taaattgtac
      121 gttgtgatac ttccattaat tctgacaact catgagcact tacacgtttt ctctcattca
      181 aaatcaaaat aatactaatt aaccgactaa ttttcatcta aattccacct gccttttaat
      241 tgttgccata cagttggtca cttttatctc ttataatgat atcaaattca actacaggag
      301 gataatttat gaagaatgtt tatttttata ttatagatgg tttggcagac tgggaaataa
      361 gtaatatttt agccgagtta aactctaagc gattctttaa aaaagatgct caaacaataa
      421 acatagaaat ggtaagtaac tcaaaaacat ctattactac aatgggaggt attaatatta
      481 aacccgaatg cttaattgag gaaattagta tttctaaaga cacttttcta atactacctg
      541 gctcagacac atggaatgat tcaaaacatt tacctgtaat aaacattgct aaagaaattt
      601 tatcaaatgg tggctatgtt gcggctatat gcggagcaac aattccgata gctgatattg
      661 gtatattaaa tgaacattat catacgagta atgacttatt atatctaaaa agtttttctt
      721 caaattacca tggacaaaaa ttatatattg atcaaccatc tgtatcttat aaaaatttaa
      781 ttactgcaag ttcaactggc gcactaatgt ggacaaaaga tatactacat catctagatg
      841 tatttaaaca ttctacttta gatgcatggt ttaactattt tagtaccggc aattcaaagt
      901 attactttga attacaacaa acattaaata aagatgataa ttaatgattt aaaattatag
      961 taacatgatg gtttattata attctttttt caaaatacac gagtcatatc cctcgttaaa
     1021 aactattact aaaaatcaaa aatataaaaa aaacaaaaag tatgttttaa actttatgaa
     1081 acgtgttata taaatagtgt aatataccac aactttaaaa ggagattgag atatgaaact
     1141 aaaatcattt gttactgcca ctttagcatt gggattatta tcaacggtcg gagctgcatt
     1201 acctagtcac gaagcatctg cagatagtaa taacggctat aaagaaatga ctatggatgg
     1261 ttatcacact gttccttaca caatttcagt agatggtatt actgcattac atcgaactta
     1321 ctttatcttt tcagaaaata aaaatgttct ttatcaagaa attgacagta aagtaaaaaa
     1381 tgaattagct tctcaacgtg gtgttacaac agaaaaaatt aaaaatgcac aaacagcaac
     1441 ttatacactt actttgaatg atggtaataa aaaagtagtg aatctaaaga aaaatgacga
     1501 cgctaaaaat tcaattgatc caagtacaat caaacagata caaattgtag ttaaataata
     1561 atttgaaaat gaagaataat tacatgaggc aaacgctgaa aatttggcgt ttgtcttttt
     1621 agatattgaa attgttaatt attgttgttt gcttctccag acaatagatt ggcctatact
     1681 aaagtaaatt ctatattaat atcttccgca atttataatc taaaataaca gcaaatctca
     1741 aatatctgga acctaaataa atcccctcac tactgcttat agtgaaggga tttattttat
     1801 tacttagacg tatgctctat gacaaacatg cttaaccgta ttaaacaacc ttttatcatt
     1861 gcttaatttc caaatcgcgc attataaagt caatttttat aatccatcat tacatttccg
     1921 cattcattat tcagcttctc taatttcgtc tgcaatatct ttatagtcaa tatttgcatt
     1981 tatgaaatct tcatcttgaa ttggaaaatg ctgttcaaat gtttctatat tttggatttc
     2041 tactttaaca tcttctactt caaaatctaa gacatgtcca ccaaagttac gatcatcatt
     2101 cgcaaagtgt acatggaatc ctgctgatcc gataccatgg aatagttctg gtgtaaagaa
     2161 cccaacgact gaacctttga cgtatgtttc agtttgctca ggttgtcttc tagctgaatc
     2221 aattaaacgt gtataaggtg gctcttgtgc cggcatcata cgtacatgca tttttttaaa
     2281 taagcctgaa atttttactg cagaaaataa attttcactc aacatctttt ccttaatttc
     2341 cgcaaaaact gcttcagaag atttatcttt cgtttcatag cttgtatctg ctacaaattt
     2401 agttacagtt gcatatggtg ttaactcgtc accttttaat tccacaaatt ctttatgttc
     2461 atttgcatga taagcttttc catctaaaaa gatgacttca ccatttgaac ctgttagtgt
     2521 agcgatacct aagtcgccat gttgcaataa ttcatttatt gatgcagttc cttttaataa
     2581 acctgccatt aatgtgccta atgtaccatg ttgatataaa acattagcca taaaatttcc
     2641 cactcctcat taacatttat ctttatttta gttaaatacg tcaggtaaca tatttgttga
     2701 aagtttgata ttatctttat aatttacagg tatatcaatt aatactggac catctgtttc
     2761 atagccctct ttaattgccg cttctaattc ttcttgatta gtaactcgta aaccttttgc
     2821 gccaaatgat tcagcatatt ttacgaaatc tacaggaccg aagtcgacac ctgatgaacg
     2881 tttatatttc atttcttctt ggaattcaac catgttatat tttccatcgt tccaaataag
     2941 ttgaataatg tttagatttt tacgtacggc cgtttctaaa tcttgtgatg aaaataaaaa
     3001 gcctccatcg ccagcaacgg atacaacttg cgtattaggg cgtacaagcg cagctgaaat
     3061 tgcccacggt aatgctacac caagcgtttg cataccgtta ctaaataata aatgtcttgg
     3121 attgtaactt ctgaaattac gtgccatcca aatatagtga ctaccaacat caactgtcac
     3181 tgttgtttca tcagttaata ccttttgcat agattctata atttcaactg ggtgtaagat
     3241 gccttcttca tgcgtcgctt taataccagt ttcttgatca atatgcgctc ttaactgttc
     3301 taattcatct aaatgttgtt gatttataaa tggttcatct actttgtcag aaatcatttc
     3361 aatcgtttta gcaatattac caatcaattc ttttttcggt tgcatataat tagtaatttc
     3421 agcttgaact tcgtcaatat tgattatttg cgtatctaat tctttattcc agttactagc
     3481 ctcatactct ataggatcat aaccgattgt aacaactaaa tcacttttac gtaataattc
     3541 atcaccaact tgattacgga ataaaccaac tcgcccaaag aaatgatttt ctaattcacg
     3601 actaatcaca cccgcacctt ggaatgtttc tacaacgggt aaattggtac gctctactaa
     3661 tttgcgaata gcattcgttt cttctgcact tgaacttctc attccagcta ataaaaccgg
     3721 aaatgatgca tttttaatcg cttcaatgac atcattaata tccttttcac ttggtacacc
     3781 taaatttggt ttttgacaaa gtgatatcgc cttagattca actggtgaag aaatcacatc
     3841 ttgcggaata ctaataaaac ttgcgccatt tttccctgaa gtagcaattc gaattgcatt
     3901 tgtcataact tctgataatg attcaggatc ttgtacttct tcactatatt ttgatgaata
     3961 ttttaataaa gcagcattat caatactttg atgcgttaat cgtaataaat cattacgttt
     4021 cacttggcca cctaacgcta atacaggatc cccttcagat gtagctgtta atagtccagt
     4081 cgttaaatta cttacacctg ggccacttgt aacaagtact acacccggtt tgcctgttaa
     4141 tcttccaata ccttgcgcca tcattgcggc attttgttca tgacgagtga caataagttc
     4201 aggaccatca tcaattaaag cattaaatag atagtctatc tttgcacccg gaataccaaa
     4261 aacatattct accccattat tttttaaagt atcaataacc atatcggctg cagtgtactt
     4321 tttatcagtc atttatattc atttcctttc aaaatgtgat gtgtattaca tataattgat
     4381 ttgtgaattg taatccaatg atagttatgt gaaattaatg atatttatca aaactttaca
     4441 tatataatta gatttgtaat acataaaaat tgccttcagt acattatata tcctttgtaa
     4501 gatgaataaa aatcgttatt tgtaatacta agtattccaa aaatgcatga gtagattttg
     4561 tgcggtgtga taatgaagaa tgtcggattg attggtgtgg aggattgtta gcatatttga
     4621 ggatgaggtc tttagtcaaa gacggtttga aattgtcgtt tcatggcgga ctgaaaatgc
     4681 tgttttatga cggtttgaaa atgttgtttt tggcgattta aaattgttat ttcgataaaa
     4741 aaagccacag caagacttag atctaactgt gacttataca tttcatactt atattagcga
     4801 ttatttttat agttataaat gatagatgca gttcctaaac aaattaagaa gataatcatt
     4861 aagtatatag aaaagttatc tgttttaata taaaacctaa aaagtagaaa caccagcatc
     4921 gcaataataa aataaacagg aataaaagct ttcttccaaa atatagaatc tttcaattaa
     4981 gtcactcctt attttgataa tactttttaa ttaaggctct tgagttatcg tccggtcgtt
     5041 ctttcccttg aatatctatg attagaacac taatgtacac ttttgattgt gtaaacatgt
     5101 acaaattata cgtgtaattc aataatccat agttgaatta attctatacc tgagacatta
     5161 aataaaggaa agttcattta tactacatat atttcatatt ccccacacaa acgatcgagt
     5221 aggagaataa tcattacttg attattcgtc ctctcacacc accgagcgta cggttccgta
     5281 ctcggcggtt caatatcttg cgtaagccat ctctgcgagt tgggttagtg ataataaccc
     5341 ccacttgcgg agacgttttg ttgtaag
//
LOCUS       DAHHRR010000147         5339 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.148, whole genome shotgun sequence.
ACCESSION   DAHHRR010000147 DAHHRR010000000
VERSION     DAHHRR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.148"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            25..2304
                     /gene="secDF"
                     /locus_tag="NBI42_002384"
     CDS             25..2304
                     /gene="secDF"
                     /locus_tag="NBI42_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HCD1778771.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGMMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            2507..4780
                     /gene="recJ"
                     /locus_tag="NBI42_002385"
     CDS             2507..4780
                     /gene="recJ"
                     /locus_tag="NBI42_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCD1778772.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTSTTILVTT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKNGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     PVDVLLKENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIAVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDVFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            4802..5320
                     /locus_tag="NBI42_002386"
     CDS             4802..5320
                     /locus_tag="NBI42_002386"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCD1778773.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
BASE COUNT         1952 a          735 c          982 g         1670 t
ORIGIN      
        1 atacgtaaat aatgaggtgt tcatgtgaag aaaagtagta gaataattgc gttcttgctt
       61 ttagtcgtat tgttgtttgc cggaatggct gcaacttata aaagtgttat aaagaatgtc
      121 aatttaggac ttgatttgca aggtggattt gaggtgcttt atcaagtcga tcctttaaat
      181 aaaggcgaca aaatagataa aaaagcctta caatcaacag cgcaaacatt agaaaatcgt
      241 gttaacgttt taggtgtttc cgaacctaaa attcaagttg aagaacctaa tagaattaga
      301 gttcaacttg ctggtgtaac agaccagaat gaagcacgta aaattttatc ttctcaagct
      361 aatttaacaa ttcgcgatgc agaagataaa gtaaaattaa gcggttctga tattaaacag
      421 ggatctgcca aacaagaatt taaacaggaa acaaaccagc ctacagttac ttttaaagta
      481 aaagacaaaa acaaatttaa aaaagtaacg gaagaaatct ctaagaaacg tgataatgtc
      541 atggttgttt ggttagattt caaaaaaggt gacagctata aaaaagaagc acaaaagaaa
      601 aaccctaaat ttatttctgc agcaagtgta gatcaaccaa tcaattctga cagcgtagaa
      661 atctctggtg gatttaaagg acaagaaggc gttaaaaaag caaaacaaat cgccgaattg
      721 ttaaatgcag gttctttacc agttgacttg aaagaaattt attcaaactc agtaggtgcc
      781 caatttggtc aagatgcatt agataaaact gtgtttgcat cgtttattgg tgtagcatta
      841 atttatttat tcatgcttgg attctaccgc ttacctggtt tagtagcgat tattgcattg
      901 acaacttata tctatctaac gttagtagca tttaatttca tttctggggt tctaacttta
      961 ccaggattag cggcgttggt attaggtgta ggtatggctg tagatgccaa tattatcatg
     1021 tatgagcgta ttaaggatga acttcgaata ggtagaacga taaagcaagc cttttctaaa
     1081 gcaaacaaaa gttcgttctt aacaattttt gattctaact taacaacagt tatcgccgca
     1141 gcagtattat tcttcttcgg tgaaagttca gttaaaggtt tcgcgacaat gttattatta
     1201 ggtattctaa tgatctttgt tacagccgtg ttcttatcaa gattcttatt atcattactt
     1261 gtttcatcaa atatattcaa aaatcaattt tggttatttg gtgttaaaaa gaataaacga
     1321 catgatatta atgaaggtgt agatgttcat gaccttaaaa cttcattcga gaaatggaat
     1381 tttgttaaat tagctaagcc attaattgga gtaagtattt taattgtagt tgtcggttta
     1441 gttattcttt atatcttcaa gttaaaccta ggaattgatt tctcaagtgg tacacgtgta
     1501 gatttccaat ctaaacaagc aattactcaa caaaaggttg agcaagttgt aaaagatagt
     1561 ggacttaaag cggatcaaat tcaaatcaat ggtaaagata ataaagtagc aactgttcaa
     1621 tttaaagatg atttaacgcg tgctcaagat aataaattga gtgataatat taaatcgaaa
     1681 ttcggtgaca cacctcaaat taatactgta tcaccaatta ttggacagga attagcaaaa
     1741 aatgcaatgc ttgcattaat ctatgcatcg ataggtatca tcatctatgt atcattacga
     1801 tttgaatggc gcatgggtct ttcatctgta ttggcattat tacatgatgt attcattata
     1861 gtagcgattt tcagtttatt tagaattgaa gtagatttaa catttatcgc cgctgtatta
     1921 acaattgtcg gttattcaat taatgataca atcgtaacgt ttgaccgtgt acgtgaaaac
     1981 ttacaaaagg ttaaagtgat tacgacaaca gaacaaattg atgatatcgt taatagatca
     2041 attagacaga caatgacacg ttcaattaat acggtattaa cagttattgt agtagtagtt
     2101 gctatactat tcttcggtgc tcctacgata ttcaacttta ctttagcatt atttatcgga
     2161 ttgatttctg gtgtattctc ttcaatcttc attgccgttc cgctatgggg aatgatgaaa
     2221 aaacgtcagt tgaaaaaatc gccgaaacac aaattagttg tatataaaga aaaaaaatcg
     2281 aacgatgaaa agattttagt ttaaaatgaa ttaagcggta tgtgaaacaa taaagagttc
     2341 acataccgct ttttactttg tttaaattta acaaaatata tataaataag ttcccgcatt
     2401 catttttaaa aaatggtata attaaagtct tgattattta gataagtgct ttttatttaa
     2461 agtaatttta gatataatgt cttttggaaa tgaggcgtaa atatatatga ttaaaccgaa
     2521 gtataagtgg aaattaacta aaccagctga atacataagt gatgaattaa catcaaaatt
     2581 aaagttaacg ccaatcgtaa aaaaaatatt agaaagtaaa tcgattattg atgaacaagc
     2641 aattgaatcc atcatttcag atacagatat caatcatgat gcactgcaat tgagtgatat
     2701 gactaagact attgaaagaa ttaaaagagc gatagcgaat gatgagaaaa tcctagttta
     2761 tggtgattac gatgccgacg gtgttacctc aactacaatt ttagtgacaa cattacaact
     2821 tttaggtgca caagtaggat ggcatattcc taaccgcttt acggaaggat atggaccaaa
     2881 cgaactagca tttagaaatg ctcatgatga aggcataaca ttaatcataa ctgttgataa
     2941 tggcatacaa ggacataatg aaattaaaat ggttcaagat ttaggagttg atgtcattgt
     3001 aactgatcat catgaaattg gtagtacatt accagaagca tatgcaatcg tacatccaat
     3061 gcatccatcg tttaattatc cgttccaaca attgtgtggt gcaggtgttg cgtacaaatt
     3121 agcacaagca ttgattgaaa atgtacctga ttattttaaa gcattagttg cgataggtac
     3181 cattgcggat ttagtatcct taactgatga aaatcgatca ttagtgaaac aaggattaaa
     3241 agtcttaaat gatcaatgtc cgacctctgt taaagcattg ctaaaagaag cgggatacaa
     3301 tgacaatatt gatgaagaaa caattggttt tattattgga cctagactta atgctgtagg
     3361 tcgtttagat gacgcatcat tagcttgcga attgttgatg actgacgacg aagaagaagc
     3421 tgcattttta gcagaacaag ttgaacattt taaccgtgaa agaaaagata ttgtagcaac
     3481 tatcacagaa gaagcaatgg ctatggcaga aacgaaagtt aaaaatggag atttattttt
     3541 acttttagca aaagaaaatt ggcatgaagg tgtcttaggt attgttgctt cgaaaattgt
     3601 tgaaacattt gctttgccaa cattaatttt aaatattgat agggagcaaa atcatgcaaa
     3661 aggttccgcc agatcaattg atcaagtgtc gatgtttgaa atactatcag cacaccaaga
     3721 gttaatcgct aaatttggcg gccatcatat ggctgcaggt atgacgatgg atattgaaaa
     3781 tatagaatcg ttagctgaag gtttaaataa atggatgaaa gaattatcta aaacaacttc
     3841 gttagatccc gtaaagccgg tagatgtatt attaaaagaa aatgatataa cgattaaaaa
     3901 tattcgtgat atgaatcgtt taagaccctt cggaacagat ttttctagac ctatttttga
     3961 aatggatgat ttatcagttt cttcagttaa ggcgataggt caacaaaaaa atcatctaaa
     4021 attgacactt ggtgaaagta atatcgccgc actattttgg caaaatggac acttagaacc
     4081 tgagttacaa gatgaacaac caattaatat attaggttct gttcaaatca acgaatggaa
     4141 tggtaatcaa tcaccgcaat taattattca agatattgcg atgaacgaac agcaaatatt
     4201 agattataga agtaagcgaa aaagtttacc ttttacagaa aatgatgaaa atattgccgt
     4261 gcttattcat cctaaaagtg ataaagtaaa tgcgaatgaa tattattatg gtgaagaaat
     4321 taaacaacaa actgataaag tagtattaag agatttacca acgtcaatgg aagatttgtc
     4381 taattccctg caacaactgc aattttctca actttatata gttttgcaac ataatcattc
     4441 gatttacttc gatggtatac ctaatatgga tgtttttaaa aagtgttata aagcattaat
     4501 aactaaacaa gaaacaaata tccagaaaga gggtatgtta ttgtgtcaac atttaagtgt
     4561 gaaaccagat acacttaaat tcatgttgaa agttttctta gacttaaaat ttgtaacaca
     4621 agaagatggt ttaattcgaa tcaatcaaca acctgataaa agatcgattg attccagcaa
     4681 agtatatcaa ttaagacaac aacgtatgga tgttgaaaag caattattat atcaagattt
     4741 ttcagaaata aaaaattgga taaagtcaca attgtcgtga gcaatttagg aggaaatatt
     4801 aatggattta aagcaatacg tatcagaagt tcaagattgg ccgaaaccag gtgttagttt
     4861 caaggatatt actacaatta tggataatgg tgaagcatat ggctatgcaa cagataaaat
     4921 tgtagaatac gcaaaagaca gagatgttga tatcgttgta ggacctgaag cgcgtggctt
     4981 tatcattggc tgtcctgtag cttattcaat ggggattggc tttgcacctg ttagaaaaga
     5041 agggaaatta cctcgtgaag tcattcgtta tgagtatgac ctagaatatg gtacaaatgt
     5101 tttaacaatg cacaaagatg caattaaacc aggtcaacgt gtgttaatta cagatgattt
     5161 attagctact ggtggtacga ttgaagcagc aataaaatta gtcgaaaaat taggcggtat
     5221 cgtagtaggt attgcattta taattgaatt gaaatattta aatggtattg aaaaaattaa
     5281 agattacgat gttatgagtt taatctcata cgacgaataa aaattaatat aattttatc
//
LOCUS       DAHHRR010000148         5219 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.149, whole genome shotgun sequence.
ACCESSION   DAHHRR010000148 DAHHRR010000000
VERSION     DAHHRR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.149"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(471..1418)
                     /locus_tag="NBI42_002387"
     CDS             complement(471..1418)
                     /locus_tag="NBI42_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD1778774.1"
                     /translation="MKRSSKSKMSLPKKIFLWAFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRSEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            complement(1551..2348)
                     /locus_tag="NBI42_002388"
     CDS             complement(1551..2348)
                     /locus_tag="NBI42_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCD1778775.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLITEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNKMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            2733..3425
                     /locus_tag="NBI42_002389"
     CDS             2733..3425
                     /locus_tag="NBI42_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCD1778776.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            3622..3981
                     /locus_tag="NBI42_002390"
                     /pseudo
     CDS             3622..3981
                     /locus_tag="NBI42_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4072..4809)
                     /gene="sdpB"
                     /locus_tag="NBI42_002391"
     CDS             complement(4072..4809)
                     /gene="sdpB"
                     /locus_tag="NBI42_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HCD1778777.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFDY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            4858..5187
                     /locus_tag="NBI42_002392"
     CDS             4858..5187
                     /locus_tag="NBI42_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778778.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYLINENQVSYYEIITLLNKRPLRQVDYGNKIQYLNFYHTELSNAL
                     FAIKFAH"
BASE COUNT         1764 a          934 c          668 g         1853 t
ORIGIN      
        1 ttaagcgtat ttttgtattt tttatttttg caatcatttt tatttgaaat attttgcgcg
       61 aaattaaatc atctttttgt ttaattgaaa ataattatca ttattagctt tccaattatc
      121 tgtttcacgc ttttttgcca tttctttcac aaccttatta atgacaatac ttaataatca
      181 cctcacctaa aaatcgctat actatttata aatacccttt ttctgaaaat taataaccca
      241 agtttgataa atatctactg tcatttagaa ggtaagattt atctttaaat taaatttgta
      301 atggattaat taataaaaat caaatcagac attaaataaa atagcccata aatacaaagt
      361 gttatcacct tctatttacg ggctattaat tctattcgtt attctttata cagctcattc
      421 tatctaatta atttgtgtac aattttgata acttattttc ccttagttta ctactctaaa
      481 ttatctttta ataacttagt actttcagct tttgactgct cactaggaat gaagtagtac
      541 aatccgtcac tttgaatgcc gccttgacca ctcaattgat gtttattaat cgtgtcatta
      601 gcatctttat aattgcttct aatcgtattc aaatcaccta atgttaaatc tgttttaaca
      661 ttattttgaa tttcattcat tagactatta aaatgtgtaa tcgatgatgg gcttgcaatc
      721 ttattagcca tcgcttcaag tacaatttgc tgacgttgtt gtcgaccaaa gtcaccacca
      781 gcaccttctt ctttacgact tctaataaac ttcaatgctt gatcaccatt tacatgcgtt
      841 tgctgtcctt ttgtaaaacg aacaccatca acagtgaatg tatcattact tactacatca
      901 acaccgccga tgctatctat catattatgc aaaccatcca tatcgattgt cgcataatga
      961 tcaattggca cattcattaa tttttcaagt gatttaacag ccatatttgg tccaccatat
     1021 gcataggcat gtgcaatttt ttcagtagta ccacggccaa caatttccga tcttgtatca
     1081 cgcggtatac ttactatttc agttttcttc gttttagggt tgatagataa aatcataata
     1141 ctatcactac gctctccgcc accctttttc ttacgatcag catctgaatc gacaccaaat
     1201 aaagcgattg tgaatggatc accatcgttt aaactcactt ttttatctct taattctgaa
     1261 tgattgcgat ctaacggatt gtgtatctta ttaccagtaa taaaaatttt agcagctaca
     1321 tacacaacag ctacaatcgc taagattact aaaataccga atgcccataa aaatattttt
     1381 ttcggtaggc tcattttact tttagacgaa cgtttcaatc ccaccactcc tttactattc
     1441 cttacatact ttgtctgttt tctctattta ttatatagta aaataatttt tttactatac
     1501 ttctgtagac gtataactat tttttattat tttttatctc tcgagaatat ctatctgtat
     1561 ttttgataac caccatttgc atttaaaatt ttaagcaccg tttcatgaca tgctttatta
     1621 cttataataa aaggtgcacc ctttaaatga tcaattgcct taccatctaa agttgtcatt
     1681 tttagattca atagttctgc aaataaaaac tgtgcagcaa tgtcccaagg tttaggattt
     1741 gtattaatat gtgccccaaa ttgacctttt gccactcgca tagaatctaa tccgcaagca
     1801 ccaactaaac gataactaaa tgaggcgtca aataaatctt gcaccgtatc cagattcatc
     1861 acttgtgcat taaacgatat aatagcgtct tccaatttta acgatggtgg ttcttccatc
     1921 ttaattccat tacaaaaagc accttctcct cgtattgctt tataaagctt tttatgcgga
     1981 taatcatata cgtacgataa cattggttta ccttcataaa aatacgccaa tataatacaa
     2041 taatcttctt gctgttttac taaattggca gttccatcaa tggggtccat aatccataaa
     2101 tgattaattt cattcgtaat cattttatta cttttttctt ccgctaatag ttggtgttcc
     2161 ggaaaatgtg ttgctaaaaa ttgttgaaat tgttgttgaa tctgtttatc tacatttgta
     2221 actaaatcaa atcgatgacg cttcgtttct gtagtcatct ccgtaattaa ttgtggaata
     2281 acattgtcta tttgtttcaa ccatgaacat attaacttat ctatttgctg taatgtttta
     2341 tctgtcattt cgtccaccac ttctcatatc attatcattt tattattacc ctatattaaa
     2401 agaatcaaca atacaactga agacttcttc attttatgca taaaaaaatc ggctagtcac
     2461 gtgctagccg acaaatagaa aggaaagtaa gtaataaata ttgaagatgt tgtgatgtaa
     2521 cttgaacgat taaaagctat ctgttatata gctctacccc tttgtttaat cgctccccct
     2581 gttacaagta atatcatagc acaatctttt ttaaaatgta agcgttttcc acaaagtttt
     2641 tccgattttt ttaaaaagat attgaaaatg tcctcattgt cactcttatg ttatactttg
     2701 tgtaatatat catcttttag gaggtggctg tcatgaataa agctgaaagg caaaatttaa
     2761 taattactgc aattcaacaa aataaaaaaa tgaccgcttt agaattagct aaatattgca
     2821 acgtatccaa acgcacaatt ttaagagata ttgatgattt agaaaatcaa ggtgttaaaa
     2881 tttatgcgca ttatgggaaa aatggtggtt accaaataca acaagcacaa tctaaaattg
     2941 cattaaactt atctgaaaca caattatcgg ccttattttt agtgcttaat gaaagtcagt
     3001 cgtactcgac attaccatat aaaagcgaaa tcaacgcaat tataaaacaa tgtttaagtc
     3061 ttccacaaac acgcttaaga aaattgctta agcgcatgga cttttatatt aaatttgatg
     3121 acacacaaca tatgacactc ccaatgttgt tttccgacat tttaatctat tgtacagaac
     3181 gaaatgtgat gttagtagat catagggttg atgataatat taaagccgaa aacgttatat
     3241 ttattggcct tttgtgtaaa catggacatt ggcatgcagt catttatgac attgctcaag
     3301 acaaaactgc cgaactcgaa attgaaaata ttatagatat ttcgtattca ttcggtaaga
     3361 cgattcaaac cagagacata tccattgata actatcatca atttttaaac cccatcgatt
     3421 cctaaaaaac agcagtaaga tgattttcaa ttagaaaata tcttgctgct gttctctatt
     3481 tatacaatac ttcgtattga atggcttcgc tttcctaggg tgccgtctca gccttggtct
     3541 tcgactggca ctgctccctc aggagtctcg ccattaatac tacgtattaa catgtaattt
     3601 tactttgaaa tacttaaaaa aataaaacac tttgcccaac ttacactacc aatagaaact
     3661 gctgttagaa ttcctcaaaa tgatatttcg cgatatgtta atgaaattgt taaaaagata
     3721 gctgatagcg aattcgatga attcagacat catcgtggcg caacatccta tcatctaaaa
     3781 atgatgttaa aaatcacctc atattcatat actcaatctg aattttctgg ccgtagaata
     3841 gaaaaattac ttcataacag tattcgaatg atgtggttag ctcaagatca aacaccttct
     3901 tataaaacta ttaatctttt tagagtgaat cctaatactg atgcgctaat tgaatcttta
     3961 tttattcagt ttcataataa aatgcatatc aaaaaagctg atttctatca aataattaat
     4021 agaaatcagc ttttttcatt gcctaaaaac ttaatgtccc gacctcttta tctatgcata
     4081 aatacttatt actgatataa cgaaagaaac aaaattattt gctatatgta atgcaattgt
     4141 tgaacctagg tttcttccag attttaaata agtgaaaact aatatgatgg atagtatgag
     4201 atatggacca aactcaaacg gcgactttgc atcagtcaca tgaataaatg caaataagaa
     4261 caccgaaaca atactcatag ctataaaatt aaacttctta cctaattctc caattaaaat
     4321 atgtctaaat acgatttctt caactattgg acctacaatc acaattaata agaatgctac
     4381 aggtaaaaat gcaggcactt caaacatttt atttagctca agttcattgg ctgtttcact
     4441 atattgcaaa tgtttaggta gaaactgtgt catatattca tatgtataaa ttaagatgag
     4501 agcaataata tacgttattg acaatctaag ccaatatttt ttgatatacg caaaaccagc
     4561 tcgaagcctt gatggcatca cttttaaatg aaataaataa aatgcgccaa tcccaattgt
     4621 atatgctaaa gcttgtgtga tagtcgctac aaatatcaga ttactatcga tttcataata
     4681 atcaaacaaa attggtccta tgtaagctgc aattgtgagt gcataaaata taacacctat
     4741 aattggaatt ataagcaaat ctctccatgc tatatcttta aacgtgtatt tctttttttc
     4801 attttccact gttatatcct ttcctgttta ataattgatt tttggaggta cttctacatg
     4861 ataaacgaaa ctaagtatat gagacaacaa attactaatt tgattcaaat cattgatacg
     4921 attaaatata atactttaat gacagattgg aacattcaaa cgcatattta taaatttaac
     4981 caaatagtta ctaatgaatt gaataagttc aaaggctttg aaacatcata tttaataaac
     5041 gaaaatcaag tttcctatta tgaaattata acactactta ataaacgtcc cctccgacaa
     5101 gtcgactatg gtaacaaaat tcaatatctt aatttttatc atacagaact ttctaacgca
     5161 ttattcgcaa ttaaatttgc tcattaacct atttttcata aaatgtcatt taaacaagt
//
LOCUS       DAHHRR010000149         5029 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.150, whole genome shotgun sequence.
ACCESSION   DAHHRR010000149 DAHHRR010000000
VERSION     DAHHRR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.150"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..123)
                     /locus_tag="NBI42_002393"
     CDS             complement(<1..123)
                     /locus_tag="NBI42_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778779.1"
                     /translation="MIIQCSIVSNFIFNLYIVTNFNLNFLSLKQIIYSKLIVRKF"
     gene            161..622
                     /gene="tsaE"
                     /locus_tag="NBI42_002394"
     CDS             161..622
                     /gene="tsaE"
                     /locus_tag="NBI42_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCD1778780.1"
                     /translation="MIKINNLDEMNQFAMFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            603..1265
                     /gene="tsaB"
                     /locus_tag="NBI42_002395"
     CDS             603..1265
                     /gene="tsaB"
                     /locus_tag="NBI42_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCD1778781.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDTIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWKNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGDVIAQLPNASVMYQLIDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1238..1702
                     /gene="rimI"
                     /locus_tag="NBI42_002396"
     CDS             1238..1702
                     /gene="rimI"
                     /locus_tag="NBI42_002396"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCD1778782.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDIERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIEDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1695..2720
                     /gene="tsaD"
                     /locus_tag="NBI42_002397"
     CDS             1695..2720
                     /gene="tsaD"
                     /locus_tag="NBI42_002397"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCD1778783.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITTTINEALVDADVSMEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLTFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLGLNGHSNID
                     LEEYSAE"
     gene            complement(3087..4697)
                     /locus_tag="NBI42_002398"
     CDS             complement(3087..4697)
                     /locus_tag="NBI42_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HCD1778784.1"
                     /translation="MSTNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRHNIL
                     FLCPFLPIISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADSVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVSNG"
     gene            4786..4914
                     /locus_tag="NBI42_002399"
     CDS             4786..4914
                     /locus_tag="NBI42_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778785.1"
                     /translation="MIKTMSIENMRTYQQRVYNLWIKGVDNVIYCGYQVGKQKIND"
BASE COUNT         1738 a          702 c          928 g         1661 t
ORIGIN      
        1 aaattttctt acaattaact ttgaatagat aatctgtttc aaagataaga aatttaagtt
       61 gaaatttgtt acaatataaa ggttaaatat aaagttagat acgattgaac actggataat
      121 catttttatg aatgaaaagc ataatatagg agaatcaaca ttgataaaga taaataattt
      181 agatgaaatg aatcaatttg ctatgttttt agttgagcaa ttgaaaagtg gtgatttgat
      241 tttacttaac ggagatttag gagcaggtaa aacaacgtta acgcaattta taggaaaagc
      301 tcttggtgta agacgtacga ttaattcccc gacatttaac atcattaaat catatagggg
      361 taaaaattta aaattgcatc atatggattg ttatcgctta gaagattctg atgaagattt
      421 aggatttgat gaatttttcg aagatcaggc aattactgtt attgaatgga gtcaatttat
      481 aaaagattta cttccagcga cgcatttatc tattaatatt tcaacaatat ctgaaaatac
      541 aagacaaatt gagttgttcg cgcaaggaga acattatgaa caaattaagg aggcaattat
      601 tcatgaattc gctgctcatt gatacatcga atcaaccatt atcagtagca ttgatgcaaa
      661 acgatgaagt tttagctgaa ataactacgg attctaaaca aaatcattca gtgcaattaa
      721 tgcctgcgat tagtcagcta tttgagcaga gcaaaatagc taaacagcaa ttagatacga
      781 ttattgttgc tgaaggtcct ggttcatata ctggcttacg tatcggtgtt actgttgcta
      841 aaacattagc gtacgcgtta gatgttaaat tatatggtgt ttcatcatta aaggcattgg
      901 cagcaacaat cgatcatact gataaattat tggttcccgt gtttgatgca agacgacaag
      961 cagtttatac aggcatattc cagtggaaaa atggtcaatt agtgactatt ttagaagatc
     1021 aatatatgtc gatagaagca ttgcgagctt ttttagaaga tttaaaccaa ccatttattt
     1081 atattgggaa agatactgta aaattacaag atgaattgca aggtgatgtg attgcgcagc
     1141 taccaaatgc atcagtcatg tatcaattga tagataaacc ttcagacgta catacattta
     1201 ctcctaaata tcataaatta gctgaggcgg aaagaaattg gatcaacagt caaaagaaca
     1261 attaaacatt agagagatga ccaaagaaga tgtgccacaa gtctttgata ttgagcgtcg
     1321 gagtttcaat gatagttctt ggacaattga tgcattttat catgaaatag agcaaaataa
     1381 ctttgcgaaa tattttgtct tagaatttga acaacaaatt attggttatt taggtttatg
     1441 gattgttatc gatcaagctc aaattacgac agttgcaatc gaagataatt atagaggcta
     1501 tggtttaggt caaatgcttt taaaatacgg aataaactat gcaagtcaca cttgtgatgt
     1561 gatgagttta gaagtaagag ttaataataa agtggcacaa catgtatatg aaaatttagg
     1621 atttcaatat ggtggtaaac gtaaaaatta ttatggtgaa ggtgaggacg caatggttat
     1681 gtgggtgaat ttaaatgact aaagatatat taatactagc tgttgaaaca agttgtgatg
     1741 aaacaagcgt tagtgttata aaaaatggca gagatatttt atcaaataca gttttaagtc
     1801 agattgaaag tcataaacga tttggcggtg tcgttcccga agtggcaagt agacatcacg
     1861 ttgaaggtat aacaacaaca ataaacgagg ctctagtgga tgccgatgta tcaatggaag
     1921 atattgatgc aatagcggtt acagaaggcc ctggactaat tggtgcgtta ctaataggtg
     1981 ttaatgcagc caaagcattg gcatttgctt acgataagcc acttattcca gttcatcata
     2041 ttgcaggaca tatatatgct aatcacatag aagagccatt aacattcccg ctaattgcac
     2101 ttattgtttc aggtggacat actgaattag tttatatgaa agatcattta acatttgaag
     2161 tcattggtga aacacgagat gacgcagtag gtgaggctta tgataaagtg gcacgaacaa
     2221 ttggtttaaa ttatccaggt ggtccacaag ttgatcggtt ggctgctgaa ggtgaagata
     2281 cttattcatt ccctcgtgtt tggttggata aagatagtta tgattttagt tttagtgggt
     2341 tgaaaagtgc cgtaatcaat caacttcaca atcaacgaca aaaaaatatt ccaatcattg
     2401 aagctaacgt agcaacgagc tttcaaaata gtgttgtaga ggtgcttacg tttaaagcta
     2461 ttcaagcttg taaagaatat ggtgttcagc gattaattgt tgctggtggc gtggcgagta
     2521 ataaaggatt acgtcaatct ttagcggatc aatgcaaagt caatgacatt caattaacta
     2581 tcccaagtcc taaattatgc acagataatg ctgcaatgat aggcgttgcc ggccactatt
     2641 tgtatcagca aggtcgattt gctgatttag gattaaatgg gcacagcaat atagatttag
     2701 aagagtattc tgcagaataa aataaacgtc agttattaac atgattacga tgaacattat
     2761 tgaaactgtt cactgtcttc tgtcatgtga tagctgacgt ttttatattg caattagggg
     2821 agtgagacag aaaagatatt ttcgcaaaat ttatttcgtc gtccaacccc aactcgcatg
     2881 gcctgtagaa tttcatttcg aaattctctg tgttggggcc cacaccccaa ctcgcattgc
     2941 ctgtagaaat tggtgttcca atttctctat gttggggccc cggactagta ttgaaaaaac
     3001 ttattacaag cgcattttca tttagtcaaa gactgccaat ataacaatgt tgcgacttga
     3061 acattgattg atgttcaaga ttcattctaa ccattggaaa cattatcttt tgctcgttca
     3121 tatatttttg ctggaaatga agtgattttt aataattcga tggcattacg tgtatttgct
     3181 ttgccaggct taattttgta atcaaaatgt atgttattat tttctattac ctcattgaaa
     3241 tgataatttt cataacgttg ttttaagagt tcagctaact caatatcatg tgttgctgca
     3301 ataactctaa agttagattt ttcatgtaaa aatgatagta ctgattctga agcggcaatt
     3361 cgttcagttg tgttggtacc tttaaaaatt tcatcgataa agcagtatat tttttgatta
     3421 tcaggaattt caacaatacg cttaatagat tttaattcag ccataaaata actatctcct
     3481 gataacacat catcggcatt tgccattgac gtgaaaacaa ttccaggttg atatacaaat
     3541 ttgcttgcgg taactgtttg tatagccgat gcaagaataa tgtttattgc tatggatttc
     3601 ataaatgttg atttacctga agcattagat ccagttaaca gtatattttg tgatagtgag
     3661 aaatcattgg ctactgaatc tgctatgagt ggatgtgtta actcagagaa tactatgcca
     3721 tcatttgaat catcaatttg tggttcagta tagcattcta gcgttctacg atacattgct
     3781 aatgcataat gattatctaa ttcagctatg taatcgaaac aattctttag ctcattcata
     3841 tgtgttgtat agcttttttg aattgaatgg aataaaacat aatcaagcat gaaaataagt
     3901 ttaactagtt taattaccat agctccgata tcttcatcat taacttcagc taaaacactc
     3961 gttaaatgac gtgctgttct aaattgctta aaattgactt cgggttgtgg agcatgttta
     4021 atttttgaga tagtatagcc ttgttttaaa acattggatg cataaaagat agactttaag
     4081 tcatcctcat atgtacgctt taaagttgca gataaaatga tattaaatag gcaactcata
     4141 agaaataata agataccaac ttgtgaatta ataaatataa ctgcaaatga gataattggt
     4201 aaaaacgggc atagaaacaa tatattatga cgttttaccg gtgtgatttg gtctggaaat
     4261 gttggataaa cagttttacc aatcaaagct aaatgatatg ttacgttttt tctaaattca
     4321 gcattatcat taaataaact aagtaatgat ttgttcgtaa ttgaaagcat tccacgtaaa
     4381 caggcatata gtttcatttc accaatagct gttaaattat aattcatctt atgaaagatt
     4441 gcattcatat ttaagtcgga ccaagtttta tcatcaacta atgaatgtga ttcaaatttt
     4501 tccttgtaga gcttatactc atagtcgaaa tgtgagttcg gtctgataaa agcttctaat
     4561 ggagaaatcg tttgccataa tgtttccata gattttaata gacgttgacg actcatgtat
     4621 cgtccaacaa ttcctattac agatgtaagt agaataattg ctataacaaa tattaaaaat
     4681 gtttgatttg tgctcataag tatcccctta tttattttta ataataataa aaatatactt
     4741 caatattatt attatttcat aaagaaaatg tattaacaat ggattttgat aaaaacaatg
     4801 tcaatagaga acatgcgtac ttatcaacaa agagtgtata acttgtggat aaaaggtgtt
     4861 gataacgtca tttattgtgg atatcaggtg ggaaaacaga aaataaatga ttgattagtg
     4921 ttaatttact tgtggataac taggataact ctgtggatag ctataatatc agcgttttta
     4981 ttgtgaataa aaaaatttaa aatttgtaaa aaaataactc taactatag
//
LOCUS       DAHHRR010000150         4826 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.151, whole genome shotgun sequence.
ACCESSION   DAHHRR010000150 DAHHRR010000000
VERSION     DAHHRR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.151"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..289
                     /locus_tag="NBI42_002400"
     CDS             <1..289
                     /locus_tag="NBI42_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCD1778786.1"
                     /translation="YIDPLLSTNRIFEKLKMTIKLRVHESIVERLLDFCDYDSFRQEN
                     NRYYVVDFPFIDNSYYFGLILSFGDKCEVIEPTEIRNKMKQTIKKMADNYK"
     gene            complement(463..2880)
                     /locus_tag="NBI42_002401"
     CDS             complement(463..2880)
                     /locus_tag="NBI42_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HCD1778787.1"
                     /translation="MTYRMKKWQKLSTITLLMAGVITLNDGEFRSVDKHRIAVADTNT
                     QTPNYEKLRNTWLDVNYSYNQYDEKNDAMKKKFEATEKEAEKLLKEMKTESGRTYLWA
                     GAENLETNSSHMTRTYRNIEKIAEAMKHKNTKLKTDENKTKVKEALEWLHKNAYGKEP
                     LKKLEELKTNFSKSAPQKNTNLNWWDYEIGTPRALTNTLLLLNGDISNDEKKKYTAPI
                     KTFAPNSDKILSSVGQPEQAKGGNLVDITKVKLLESIIEEDKDMMKKSIDSFNKVFTY
                     VQDSATGKERNGFYKDGSYIDHKDVPYTGAYGVVLLEGISQMMPMIKETPFKETSQKD
                     TILKSWIDDGFMPLIYKSEMMDLSRGRAISRENETSHTASATVMKSLLRLSDAVDEST
                     KAKYKKIVKTSVKSDSSYKQTDYLNSYSDIDKMKSLMADSTISTNGLTQQLKIYNDMD
                     RVTYHNKDLDFAFGLSMTSKNVARYESINNENLKGWHTGAGMSYLYNSDVKHYRDNFW
                     ATADMTRLPGTTTLNDMPSTNTKNNNSFVGGTKLNNKYASIGMDFENQNKTLTAKKSY
                     FILNDKIVFLGTGIKSTDSSKNPMTTVENRKANEYTMYKDDKQTTASNDEETNSVFLE
                     STDNKKNIGYHFLNKPKITVKKESQTGKWSEINKSQKSDDKKDEYYEVTQKHSNTDSK
                     YAYVLYPGITKDNFKSKASQVSIVKQDDDFHVVKDNESVWAGVNYSDSAKTFEINGTK
                     VEVKDKGMFILKKKDDKTYECSFYNPESTNTASDIESKISMTGYSITNKNASTTNESG
                     VRFELTK"
     gene            complement(2877..3044)
                     /locus_tag="NBI42_002402"
     CDS             complement(2877..3044)
                     /locus_tag="NBI42_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778788.1"
                     /translation="MRKIWVIDCLYTSTLFADCLRVDSYLYALTNFEKLNHYLIRLLI
                     AHAQHKKETCQ"
     gene            3289..3705
                     /locus_tag="NBI42_002403"
     CDS             3289..3705
                     /locus_tag="NBI42_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1778789.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     ARDLLQYKIDNYDNHIAKINASDNHDANVERLWERK"
     gene            3775..4623
                     /locus_tag="NBI42_002404"
     CDS             3775..4623
                     /locus_tag="NBI42_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1778790.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRLIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVVLRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHAEPEMIKALT
                     SRELDV"
BASE COUNT         1591 a          745 c          769 g         1721 t
ORIGIN      
        1 gtatatagac ccattattat ctacaaatag gatctttgaa aaattaaaaa tgacaattaa
       61 acttcgcgta cacgaatcaa ttgttgaacg tttattagat ttctgtgatt acgatagctt
      121 taggcaagaa aataatcgat attatgtagt tgacttccca ttcatcgaca actcatatta
      181 ttttggatta attttaagtt ttggagataa gtgtgaagtt atagaaccaa ctgaaattag
      241 aaataaaatg aaacaaacaa ttaaaaaaat ggcggataat tataagtagt cagattacaa
      301 taattaaatg aggagaaact tgctgaatgg cttggtaaat cgatttataa ataatcgaga
      361 acttttcaag ggtgatgtgg tctgcggatt acaaggtgaa tgatagatat atttataaaa
      421 tctcgacact ataaaaatgg tatagtgccg agattttctt gcttatttag ttaattcaaa
      481 gcgcacgccg gattcatttg tagtcgatgc atttttattt gtaatagagt atccagtcat
      541 tgaaatttta gattcaatat ctgaagcggt atttgtagat tcgggattat agaagctaca
      601 ttcataagtt ttatcatctt tctttttaag aataaacatt cctttgtctt taacttcgac
      661 tttagtacca ttaatttcaa aagttttagc gctatcacta taattgacac cagcccaaac
      721 cgattcatta tctttcacaa cgtggaagtc atcatcttgt ttaactatag atacttggct
      781 tgccttggat ttaaaattat cttttgttat tccaggatac aggacatatg catatttact
      841 atctgtatta gaatgttttt gagttacttc ataatactca tcttttttgt catctgattt
      901 ttgactttta tttatttcac tccacttacc agtttgactt tcttttttta cagttatttt
      961 cggtttgttt aaaaaatgat agcctatgtt ctttttgtta tctgtggact ctaaaaagac
     1021 tgaattggtt tcctcatcat ttgaagctgt tgtttgttta tcgtctttat acattgtata
     1081 ctcattcgct ttgcgatttt caacagttgt cattggattc tttgatgaat cagtactttt
     1141 aatgccagtt cctaagaaga caattttatc gtttaatatg aaatatgatt ttttggcagt
     1201 taaagttttg ttttgatttt caaaatccat accgatgctt gcgtatttat tatttaattt
     1261 tgttccgcca acaaaggaat tattattctt agtatttgta gatggcatat catttaaagt
     1321 agtagtacct ggaagacgag tcatatcggc tgtcgcccag aagttatcac ggtagtgttt
     1381 cacgtcgctg ttgtataaat aagacatgcc tgcaccagtg tgccaacctt ttaggttttc
     1441 attattgata ctttcgtaac gtgcgacgtt tttcgacgtc atacttaaac caaatgcaaa
     1501 gtctaagtct ttattgtgat aggtgacacg gtccatgtca ttatatattt taagttgttg
     1561 tgttaaaccg ttagtagaaa tagtgctatc tgccattaaa gacttcattt tatctatgtc
     1621 tgaatatgaa tttaaataat cggtttgttt ataacttgaa tctgacttta ctgaagtttt
     1681 gacaatcttt ttatatttag cttttgtaga ctcatccaca gcatcactca atctcaacaa
     1741 tgatttcatt acagttgcag atgctgtgtg actcgtttca ttttcacgac tgatagctct
     1801 accacgtgat aaatccatca tttcactttt ataaattaat ggcataaatc cgtcgtcaat
     1861 ccatgatttt aaaatagtat ccttttggct agtttcttta aaaggtgttt cttttatcat
     1921 tggcatcatt tgagaaatac cctctaagag tacaacgcca taagcaccag tgtatggaac
     1981 atctttatgg tcgatatatg agccgtcttt atagaatcca ttacgttctt taccagttgc
     2041 agaatcttga acataagtga acactttatt aaaagaatct atagactttt tcatcatatc
     2101 tttatcttct tcgataatac tttctaaaag tttcacctta gtaatatcta ctaaatttcc
     2161 gcctttagct tgctcaggtt gtcctactga cgataatatt ttgtcactat ttggagcgaa
     2221 agttttgata ggggcagtgt attttttctt ttcatcattt gaaatatcac cattcagtaa
     2281 taatagggta tttgttaatg ctctaggtgt tccaatttca taatcccacc aatttaagtt
     2341 ggtattcttt tgaggtgctg attttgagaa atttgttttt agttcttcaa gttttttcaa
     2401 cggttctttt ccataggcat ttttatgtag ccactcaagt gcttctttta cttttgtctt
     2461 gttttcatct gtttttaatt tagtattttt atgcttcatc gcttctgcga ttttctcaat
     2521 attacgatag gtacgagtca tatgagaaga attagtttca aggttttccg ctcctgccca
     2581 caagtaagtc cttccacttt cagttttcat ttccttgagt aatttctctg cctctttctc
     2641 cgtagcctca aacttcttct tcattgcgtc attcttctca tcatactgat tataactgta
     2701 gttaacatcc agccatgtgt tcctcaattt ctcataattt ggtgtttgag tattcgtatc
     2761 agctacagca atccgatgtt tatcaacact tctgaattca ccatcattca aagtaatcac
     2821 accggccatt aataatgtaa tggtggataa tttttgccat ttcttcattc tatatgtcat
     2881 tgacatgtct cctttttgtg ttgcgcgtgt gcaattaata atctgattaa ataatgattc
     2941 aatttttcaa aattcgttaa cgcatacaaa taactgtcta ctctcaaaca atccgcaaat
     3001 aatgttgatg tatataaaca atcgatcacc caaattttcc gcatcaccca aaaattgtaa
     3061 cgaaaataat tttgaaaaaa ttaaataata tcaatcttag tgattatatc attttaaata
     3121 tttaatttat ataattataa aagtaattaa aaacggtgaa atgtaaaatg ttaaaaaaga
     3181 gacaaattta aatttgagta ataaaatgag aagtattcaa acgaaggtgg caatatataa
     3241 agcaacatta ctttagatgc cccaaacata actgaaagga tagtcgcaat gaaaactaaa
     3301 gaagtcgtag cgctcatgaa tatatctcaa gacactttaa gatattatga aaaggttggt
     3361 gtgattccac ctgttaatcg agatgaaaat ggatatagaa tatataatga tagtgattta
     3421 aattggattt atttagtgaa aaatttgcga aatgcaggcg tcagtattga atcgcttatc
     3481 gaattttgta gattagcgca gcttcctaaa aatgaaaata ttcaagcaca gcaaaagcaa
     3541 attttaaata agcaactcga agaattaaat gaaaacttaa agacaattca tgatgcgaga
     3601 gatttattac aatataaaat tgataattat gataatcata ttgctaaaat caatgctagt
     3661 gataatcatg atgccaatgt tgaacgtctt tgggaaagaa agtaaaccta tggcttgcta
     3721 tagagttcgc tccatgcttt atagtggact tacaagataa aggaggttca ttttatgaat
     3781 catattgaaa taagtaaaga tgtaaagata ccagtattag gcttcggagt atttcaaatt
     3841 cctcaagaac aaacagcaga agcggttaaa gaagcaatta aagcgggcta tagacttatt
     3901 gatacagcac aaagttattt aaatgaaact gaagtaggtc aaggtattga agcttcaggt
     3961 attgatagaa gtgaactatt tattacgact aaagtatgga ttgaaaatgt taactatgaa
     4021 gatactatca aatcaatcga acgatcatta caacgattaa atttggacta tcttgatcta
     4081 gtgcttattc accaacctta taatgatgta tatggctcat ggcgtgcatt agaagagtta
     4141 aaagaaaatg gtaaaattaa agcaatcggt gtatctaact tcggcgttga tcgaattgta
     4201 gatttaggta ttcacaatca aattcaacca caagtgaacc aaatagaaat taatccattc
     4261 catcaacaag aagaacaagt tgcagcactt caacaagaaa atgttgtggt agaagcttgg
     4321 gcaccatttg cagaaggtaa aaatcaatta ttccaaaatc aattattgca ggccattgca
     4381 gataaatata acaaatcaat tgcacaagtt gttttacgct ggttagtaga acgtgacata
     4441 gttgtactcg caaaatcagt gaatccagaa cgtatggctc aaaacttaga tatatttgat
     4501 tttgaactta cagaagaaga taaacaacaa attgcgacat tagaagaaag taatagccaa
     4561 tttttctcgc atgctgaacc agagatgatt aaagcactta caagcagaga attagatgtt
     4621 taatcataat ataaaaaagt aaatcattta cattacacta acctgaactt ggatgttaaa
     4681 gtctatgttt caggttattt tattactctt aaaaagtgaa aggagcaagc aaataagtta
     4741 ttatatattt ttatattatg caataactta cctaaggtta taattagata attaatatgt
     4801 cttggaatta attatctaat aagttg
//
LOCUS       DAHHRR010000151         4759 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.153, whole genome shotgun sequence.
ACCESSION   DAHHRR010000151 DAHHRR010000000
VERSION     DAHHRR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.153"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            330..1505
                     /gene="rpsA"
                     /locus_tag="NBI42_002405"
     CDS             330..1505
                     /gene="rpsA"
                     /locus_tag="NBI42_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCD1778791.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLESEEEDNPTIGDMIGDKLKNLKL"
     gene            1728..3038
                     /gene="der"
                     /locus_tag="NBI42_002406"
     CDS             1728..3038
                     /gene="der"
                     /locus_tag="NBI42_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCD1778792.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVTVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            3055..4053
                     /locus_tag="NBI42_002407"
     CDS             3055..4053
                     /locus_tag="NBI42_002407"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCD1778793.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQYADIYLMALPTKAMREVATQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEFNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKN
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            4224..4496
                     /locus_tag="NBI42_002408"
     CDS             4224..4496
                     /locus_tag="NBI42_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCD1778794.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
BASE COUNT         1722 a          631 c          927 g         1479 t
ORIGIN      
        1 tgcttatttg cgtggctttt tgttttataa aagtataaaa ttttactata atatatcttg
       61 tagagaacaa tgaaatgaca gacttataaa acaaaatagt gagctatgct aatttttata
      121 caaaaaatat gtataaacat agcggacttt attacttgtt tgaatatatg gttgtaataa
      181 atatatgtcc aactaataca cagaatgaga ttaagtgaca tatttatgga tacaactaat
      241 ttactaaatg taataaaggt agaatttctt gacaattctg tcagtttata agatgttata
      301 aatatgtagt gtataaggag gcaaacaaga tgactgaaga attcaatgaa tcaatgatta
      361 acgatattaa agaaggtgac aaagtcactg gcgaggtaca acaagttgaa gacaagcaag
      421 ttgttgttca tatcaacggt ggtaaattta atgggattat tcctattagt caactatcta
      481 cgcatcatat tgatagccca agtgaagttg taaaagaggg cgacgaagtt gaagcatatg
      541 tcactaaagt tgagtttgat gaagaaaatg aaactggagc ttacatctta tctagaagac
      601 aacttgaaac tgagaagtct tatagttatt tacaagaaaa attagataat aatgaaatca
      661 tcgaagcgaa agtaacagaa gtagttaaag gtggtttggt tgttgatgta ggacaaagag
      721 gttttgttcc ggcttcacta atttcaacag acttcattga ggatttctct gtatttgatg
      781 gacaaacaat tcgtattaaa gttgaagaat tggatcctga aaataataga gtcattttaa
      841 gccgtaaagc agttgaacaa gaagaaaacg atgctaaaaa agatcaatta ttacaatctt
      901 taaatgaagg cgatgtaatt gatggtaaag tagcgcgttt aactcaattt ggtgcattta
      961 tagacattgg cggtgttgat ggtttagtgc atgtatctga actttctcac gaacatgttc
     1021 aaacaccaga agaagttgtt tcaattggtc aagatgttaa agttaaaatt aaatctattg
     1081 atagagatac agaacgtatt tcattatcaa tcaaagatac gttaccaaca cctttcgaaa
     1141 atattaaagg tcaattccac gaaaatgatg tcatagaagg tgtcgtagta agattggcaa
     1201 actttggtgc atttgttgaa attgcaccag gtgtacaagg acttgtacat atttctgaaa
     1261 ttgcacacaa acacattggt acgccaggtg aagtgttaga acctggtcaa caagtaaatg
     1321 ttaaaatatt aggtattgat gaagagaatg aaagagtatc actatctatt aaagcaacat
     1381 taccaaacga agatgttgtt gaaagtgatc cttctacgac taaggcgtac ttagaaagcg
     1441 aagaagaaga taatccaaca attggcgata tgattggtga taaacttaaa aatcttaaac
     1501 tataatttaa tatttaatag tcaactccac atgtttatga ttgcatgtgg agtatttttt
     1561 atgtaccaaa atatattcgg aatgataacg tggacaaatt taactaagtg ttttataaga
     1621 taaagtttta agtgctgatt tttatcatta cagtaataaa ctcattttga atacacagtc
     1681 tcatgtgata ttattaaaaa gatataagaa agagaggaag ttagcttatg actaaaccta
     1741 tagtagctat tgtaggtagg cctaatgtag gtaaatctac aatttttaat agaatagttg
     1801 gagaacgtgt ttcgattgtt gaagatacac caggtgtaac acgagatcgt atttattctt
     1861 caggtgagtg gttaacacat gatttcaata ttattgatac aggtggtatt gaaattggtg
     1921 atgcaccatt ccaaacacaa attagagcgc aggcagaaat cgccatagat gaagcggatg
     1981 ttattatttt tatggttaac gtgcgtgaag gattgacaca aagcgatgaa atggtcgctc
     2041 aaattttata caaatctaaa aaaccggtcg tattagcggt taacaaagta gataatatgg
     2101 aaatgcgtac agacgtgtat gatttctatt cattaggatt tggtgaaccg tatccgatat
     2161 caggatcaca tggtttaggt cttggtgact tgttagatgc agttgtttct cattttggtg
     2221 aagaggaaga agatccttat gatgaagata caattcgact atccattatt ggacgaccaa
     2281 acgtaggtaa atcaagttta gtaaatgcta ttttaggtga agatcgcgtt atcgtttcta
     2341 atgttgcagg gacaaccaga gacgctattg atacagagta tagttatgat ggacaagatt
     2401 atgttttaat cgatactgct ggtatgcgta aaaaaggaaa agtatatgaa tcaactgaga
     2461 aatattcagt attaagagct ttaaaagcga ttgaacgttc aaatgttgtt ttagtggtta
     2521 ttgatgcaga acaaggcatt attgaacaag ataaacgtgt tgcaggatat gcacatgaac
     2581 aaggtaaagc agtcgtgact gtcgtaaata aatgggatac tgtggaaaaa gatagtaaaa
     2641 cgatgaagaa atttgaagat gaagtacgta aagaattcca atttttagat tatgcacaaa
     2701 ttgcttttgt gtctgctaaa gaacgcacaa gattacgtac attattccct tacattaatg
     2761 aagcaagtga aaatcataaa aaacgtgttc aaagttcaac tttaaatgaa gtcgttactg
     2821 atgcaatttc catgaaccct acaccaacag acaaaggtag acgtttgaat gtcttttatg
     2881 caacacaagt tgctatagaa ccaccgacat ttgttgtatt tgttaatgat gtagaattaa
     2941 tgcatttttc ttataaacgc tatttagaga atcaaatccg tgccgctttt ggttttgaag
     3001 gtacaccaat tcatattata gctcgaaaga gaaattaacg attgggggat aacaatgact
     3061 aaaattaccg tttttggtat gggaagtttt gggacagccc ttgccaatgt tcttgcagaa
     3121 aatggacatg atgttttgat gtggggtaaa aatcaagatg ctgttgatga attaaataca
     3181 tgtcatacaa ataaaaagta tttaaaatac gcaaaattag acgttaacat catcgctact
     3241 tcagatatga ccaaagcaat tcaatatgca gatatttact taatggcttt acctactaaa
     3301 gcaatgcgag aagttgctac tcaaattaat gataagctga catctaaaaa gacttttata
     3361 catgttgcta aaggtattga aaatgggaca tttaaacgtg tgtcagaaat gattgaagat
     3421 tctatttcgc ctgaattcaa cgctggtatt ggcgtgttgt cagggccaag tcatgcggaa
     3481 gaagttgtag tcaagcaacc aactacagtt gctgcttcat caaaagataa aaatgtaagt
     3541 aaattaacgc aagatttatt tatgaatgat tatttacgtg tgtacacgaa tgatgacttg
     3601 attggtgttg aacttggtgg tgcattgaaa aatatcatcg cagtagcaag tggtatcgta
     3661 gctggaattg gctacggtga taatgcaaaa gctgcattaa tgactcgtgg cttagcggaa
     3721 attagcagat taggtgaaaa gttaggtgcc gatcctatga catttctagg tttaggtggt
     3781 atcggtgact taatcgttac ttgcacatca acacattctc gaaatttcac attaggatat
     3841 aagcttggac aaggtgaatc aatggatcaa gcattatctg aaatgaatat ggttgttgaa
     3901 ggtatttata caactaaatc cgtttatcat ttagctaaag aaaaaaatgt ggatatgcca
     3961 attacaaatg cattatatag agtattattt gaaaatatct cagtaaaaga atgcgtaaaa
     4021 gatttaatgg agcgcgataa aaaatctgaa taaaatgtgt attattacac atatacagct
     4081 aataattacg taaaatgaat agaaatggct taaattcaac gtttttgcca cataaatgat
     4141 tgcgatttat gtaatgcttg tgttaaagtt ttaccataat gatataaaca tatcattatc
     4201 aaacctcagg aggtgaatgt ctaatgaaca aaacagattt aatcaatgca gttgcagagc
     4261 aagctgattt aactaaaaaa gaagctggtt cagcagtaga tgctgtattc gaatcaatcc
     4321 aaaactcact tgctaaaggt gaaaaagtac aattaattgg tttcggtaac tttgaggtac
     4381 gtgaacgtgc tgcacgtaaa ggtcgtaacc ctcaaactgg taaagaaatt gatatcccag
     4441 caagtaaagt tccagcattc aaagctggta aagcattaaa agatgctgta aaataatttt
     4501 acttaaaaag ccctgaataa gggcttttta ttttgctttt aatacttaca acctgtacat
     4561 aaattgtaat gttcttctaa gtttttaatc tttggaacat tcattgttta agcgttcaaa
     4621 atattacatg acatcatagt tttgataaaa atagcaacta caaaatatac atatgaataa
     4681 tgagaataat tgttagcgtt gaatttactt aaaaggtaac catatctact atagtatttt
     4741 acgttattta aaatgatga
//
LOCUS       DAHHRR010000152         4746 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.154, whole genome shotgun sequence.
ACCESSION   DAHHRR010000152 DAHHRR010000000
VERSION     DAHHRR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.154"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..421)
                     /locus_tag="NBI42_002409"
     CDS             complement(<1..421)
                     /locus_tag="NBI42_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCD1778795.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTVL
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNIITTKPVQEFATDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNE"
     gene            528..1373
                     /gene="blaZ"
                     /locus_tag="NBI42_002410"
     CDS             528..1373
                     /gene="blaZ"
                     /locus_tag="NBI42_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCD1778796.1"
                     /translation="MKKLIFLIVMALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            1742..2350
                     /locus_tag="NBI42_002411"
     CDS             1742..2350
                     /locus_tag="NBI42_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1778797.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRP
                     VLQEALNFLRMGDRFVVESIDCLGRNYDEVIHTVNYLKDKEVQLMITSLPMMNEVVGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRIIYHRVVEMLEEGQSISKIAKEVNITRQTIYRIKNDNDLI"
     gene            2764..3057
                     /locus_tag="NBI42_002412"
     CDS             2764..3057
                     /locus_tag="NBI42_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCD1778798.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK
                     HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
     gene            3132..>4746
                     /locus_tag="NBI42_002413"
     CDS             3132..>4746
                     /locus_tag="NBI42_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCD1778799.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNW
                     DKSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLT
                     PSDLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLV
                     PFIFLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFI
                     ANILIIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKG
                     YKFSIFMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNI
                     RNSEEKQILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNF
                     YKNINHDFKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSK
                     NVSVFSFDRTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLK
                     DNHLEEEISGITNYKDSVLNELNETKTKMI"
BASE COUNT         1824 a          577 c          717 g         1628 t
ORIGIN      
        1 tttcgtttag ataaagtgac tgtttcttta aatatttaag atataataag gctttcaaaa
       61 atttaaaact taaaataata actaaaacta tccaaataac tgtgcagata ttatcaattg
      121 aatcccaatt aaacttatgg atatctgttg cgaactcttg aacaggtttg gtggtaatta
      181 tgttatggtt caagtcgtgt gacttacttt caactgtggg cgcttgatta ttcacattat
      241 taaattttat aaaagagaat ttaattggga taaaaggaat taatccagca agcacagtta
      301 gataccaaac tttataattt aaactataat taaaatagcg ttttaatatg tacctaaaaa
      361 atactaacaa tagaaatata aaacaaaagc ttactacact cattattagt aatttagcca
      421 ttttgacacc ttctttcaaa tatttataat aaacaattga catcaatatt acaattgtaa
      481 tattattgtt ttgtaaaaat tacaactgta atatcggagg gtttattttg aaaaagttaa
      541 tatttttaat tgtaatggct ttagttttaa gtgcatgtaa ttcaaacagt tcacatgcca
      601 aagagttaaa tgatttagaa aaaaaatata atgctcatat tggtgtttat gctttagata
      661 ctaaaagtgg taaggaagta aaatttaatt cagataagag atttgcctat gcttcgactt
      721 caaaagcgat aaatagtgct attttgttag aacaagtacc ttataataag ttaaataaaa
      781 aagtacatat taacaaagat gatatagttg cttattctcc tattttagaa aaatatgtag
      841 gaaaagacat cactttaaaa gaacttattg aggcttcaat gacatatagt gataatacag
      901 caaacaataa aattataaaa gaaatcggtg gaatcaaaaa agttaaacaa cgtctaaaag
      961 aactaggaga taaagtaaca aatccagtta gatatgagat agaattaaat tactattcgc
     1021 caaagagcaa aaaagatact tcaacgcctg ctgctttcgg caagacttta aataaactta
     1081 tcgcaaatgg aaaattaagc aaaaaaaata aaaatttctt acttgattta atgtttaata
     1141 ataaaaacgg agacacttta attaaagatg gtgttccaaa agactataag gttgctgata
     1201 aaagtggtca agcaataaca tatgcttcta gaaatgatgt tgcttttgtt tatcctaagg
     1261 gccaatctga acctattgtt ttagtcattt ttacgaataa agacaataaa agtgataagc
     1321 caaatgataa gttgataagt gaaaccgcca agagtgtaat gaaggaattt taatattcta
     1381 aatgcataat aaatactgat aacatcttat agtttgtatt atattttcta ttatcgttga
     1441 catgtataat tttgatatca aaacctgatt ttccctttat tattttcgag atttattttc
     1501 ttaattctct ttaacaaact agaaatattg tatatacaaa aaatcataaa taatagatga
     1561 atagtttaat tataggtgtt catcaatcga aaaagcaacg tatcttattt aaagtgcgtt
     1621 gcttttttct catttataag gttaaataat tctcatatat caagcaaagt gacataattg
     1681 ttgaatttat agtaactgaa acgtatgatt agggtgtata ttaattatga gatggaaatc
     1741 aatgattgtt ggctatgcga gagtatcatc gaacgatcaa aatttagaaa gacaacttga
     1801 aaatttaaag acgttttgtg ctgaaaaaat atttacagaa aaacagtctg ggaaatctat
     1861 tgaaaataga ccagtacttc aagaagcact taactttttg aggatgggag atcggtttgt
     1921 agtagaatcc attgattgtt taggtcgtaa ttatgatgaa gtgattcata ccgttaatta
     1981 tttaaaagac aaagaagtac aattaatgat tactagctta cctatgatga atgaagtggt
     2041 tggcaatcca ttactggata agtttatgaa agatttaatt attcaaatat tagcaatggt
     2101 ttcagaacaa gagagaaatg aaagtaaacg tcgacaagcg caaggaattc aagttgcgaa
     2161 ggaaaaaggc gtatataaag ggcgcccctt actttattca ccaaatgcta aagatccaca
     2221 gaaacgtatt atttatcatc gcgttgtaga aatgttagaa gaaggccaat caattagtaa
     2281 aattgcgaaa gaagtaaata taacaaggca gacgatttac agaattaaaa acgataatga
     2341 tttgatttaa taaactttgt ttcaatgcaa ctgagagctt ctcacagcgt ctcaaaacag
     2401 atgattataa tctattatga gtttttaata ataagacaag aaaagagagc gtaaatatgc
     2461 tctctctttt tctcattaac gaatgtttat aacgcaatca tgatgagagt attaatacag
     2521 ttaattattt gaaatacttt agtttatata atgttgaatt ataggtagtg aaattgtatt
     2581 tattatacat tgtcatatcg ttgtttaatg atagttaatt taaggataat agaattaata
     2641 ttatatatag atgaacaaaa caagcaacag agcagaaaaa acacttttag attattttta
     2701 ctcattctct attttattta tttttgctat aataaataaa gaatattaaa tggaggtgtt
     2761 tttatgaaaa agaaatttgt ttctagttgt attgctagta caatcttatt cggcacttta
     2821 ttaggtgtaa cttataaagc tgaagctgca acagtacatg tagctggtgg tgtttggagc
     2881 catggtattg gaaaacatta cgtatggtct tattatagtc acaataaaag aaatcatggt
     2941 tcaacagcag tagggaaata ttcatctttt agtggtgttg ctcgacctgg tgttcaatct
     3001 aaggcatctg ctccaaaggc ttggggtgga aacaaaacat tttatagtct tcactaatta
     3061 catatattaa ttaaggagaa tagacagaat taagtaaact taattctgtc tattctcttg
     3121 aaagaggcaa tatgaaaaaa ttcaaattat ttttagattt tagtacttta ttattaattt
     3181 cagggctatt atttttattt ttcttcaaag aaaatgaaga aattatacct gaaagttcca
     3241 atattttaac aatctctaat tgggataaat ctaacagtaa atcaaaagtg ttagatgtca
     3301 ttgaatcagg cgccaaaaat caaaatatac aaataattaa atccgttaaa gattttgata
     3361 ataaaaaaga attttttgtt ttcaactcaa aacgaaataa ttctgacttt atacgtaata
     3421 aaacatcact tttaactcca tcagatttat taaacagaga gataaaagga aagtattata
     3481 ttattggtga acatttcaat gtagaagagc ttacgagtga attaaaaaaa gctggtttaa
     3541 catctgaagt tgaatacata aatagaaaca tattattttt tgaattagtc atagataatg
     3601 atttattagt gcctttcata ttcttaggcg tattatatct tttgtacttc ttatatgaca
     3661 gaggatataa tttgaaattt tatgctattc aaaagctaca tggcttttca actacacgca
     3721 ttatttttca taatattcat ataaaaatca tttattggct agtacttacc gctattttct
     3781 ttattgctaa tatattaatc attcatatag ccaatttaga attggatttc ttaatgttta
     3841 ttcgtagatt tatcgtgttg ctttttgttt ttgcttgtat tactacattg ctgtggctga
     3901 tttcgtatag tcttcttttg aagttaagta ttccattaat cttaaaaggg aaaaaaggat
     3961 ataagttttc aatatttatg ggtaccttca ccaaatgtat catggtgcta atactatctt
     4021 ttttactggc acaaaattta aatgcttata cgaagttaaa gaatatatat gactctgaaa
     4081 aaatctggca aaagttagat aattactata cattagagat ttctcctaat attagaaatt
     4141 cagaagaaaa acaaatttta gaaactaata tatacaattt aattaagaaa agcgaacaac
     4201 ttggtggatt gcttttaaag aataataata tagccaatcc cgataaaaac aattacattc
     4261 ctgaaaatgg caatgtcttt tttatcaata acaattttct gaatttctat aaaaatataa
     4321 atcatgattt taaaatgatt gacaatcatt cggataaaat aaacgtgttt attcctccaa
     4381 ggttacaata tcaaaaatct gaaattcaaa aaaatcacca aggatggatt gactttcaaa
     4441 agcaacaaaa caaaaaagta gatgtgcaag tactgtcaaa aaacgtatct gttttttctt
     4501 ttgatcgcac aactaatttg aaatttgggt atctagattc tccaatagta atgttgttga
     4561 cacttgacga tttaacagga gatttttatt tagctgctgt ttctcaaggt ggatatctat
     4621 ttaaagattt agatacactt aaacagttgc ttaaagataa tcacctagaa gaagaaatta
     4681 gcggtataac taattacaag gattctgtgt taaatgaatt aaatgaaacg aaaacaaaaa
     4741 tgataa
//
LOCUS       DAHHRR010000153         4735 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.155, whole genome shotgun sequence.
ACCESSION   DAHHRR010000153 DAHHRR010000000
VERSION     DAHHRR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.155"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(50..226)
                     /locus_tag="NBI42_002414"
     CDS             complement(50..226)
                     /locus_tag="NBI42_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCD1778800.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
     gene            complement(519..1865)
                     /gene="mtaB"
                     /locus_tag="NBI42_002415"
     CDS             complement(519..1865)
                     /gene="mtaB"
                     /locus_tag="NBI42_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCD1778801.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEVKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGNTVGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            complement(1872..2624)
                     /locus_tag="NBI42_002416"
     CDS             complement(1872..2624)
                     /locus_tag="NBI42_002416"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCD1778802.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDQIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            complement(2626..3564)
                     /gene="prmA"
                     /locus_tag="NBI42_002417"
     CDS             complement(2626..3564)
                     /gene="prmA"
                     /locus_tag="NBI42_002417"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCD1778803.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQRIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            complement(3568..4707)
                     /gene="dnaJ"
                     /locus_tag="NBI42_002418"
     CDS             complement(3568..4707)
                     /gene="dnaJ"
                     /locus_tag="NBI42_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCD1778804.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
BASE COUNT         1391 a          963 c          631 g         1750 t
ORIGIN      
        1 tattttatat aattaatatt cgtgttgagg gagtcaacag aggtattaat tatttgaatt
       61 tacgtttacg tgcagcttct gatttctttt tacgttttac gcttggtttt tcgtaaaatt
      121 cacgtttacg tacttcttgg attgttccac ttttagaaac tgaacgttta aatctacgta
      181 acgcatcttc aagtgattca tttttacgta ctactgtttt agacatctgt atttccctcc
      241 ctccaaatat caacggaact ttatagtcaa ttgcacagta aaactatgca tttataagta
      301 taatatatct gtaatttatg gtcaattagt aaattgtttt ttatttgaaa catatttcac
      361 atcaaaatca caaagacttt tagattttgt tctaaaaatc tcttaataat ttatttaatg
      421 agaagagttg cttatatagt aaattgtgaa gccgttaaaa caactttaca aaacctatat
      481 ctttagtacg gaaccttatg gtatgaatca aggaatactt aaactaaaac ttctctatca
      541 gatttatttg ttgcaaaatc aacaacttta attgcttgcc cttcatttaa tggataattt
      601 gcttgcgtaa ttttaacttt tacaatttga cctatgagtg attcgtcacc ttcaaattgt
      661 actttcatat aattatctgc atatccaact aatgtaccta ctgtgttacc ctgttcctca
      721 ggaattactt caagcacatc ttgatcaaat ttagacgcat ataactttcc gagttgattg
      781 cttagcgtaa ttaacttatg cacgcgttca ttcttaactt cttcatcaat ttgatcatcc
      841 attctagcag ctggtgtacc aattctaggt gaatatggga atacgtgtaa ttcagaaaac
      901 ttgtgtttca cgataaagtc atatgtttct tggaattctg cttcagtctc tccagggaaa
      961 ccaacaatta cgtcacttgt aacagccaag tctggtaaag ctttatgcaa tttcgttaat
     1021 cgttctgaaa atctatccat tgtatactta cgtctcatac gttttaatac tgtatctgaa
     1081 ccagattgta atggaatatg caaatgacgc acaacttttg ttgaacgttc taaaacgtca
     1141 attacttcat ctgtaagttg acttgcttca attgaagaaa ttcggattcg ttctaatcca
     1201 ttaatcgttt caagatcacg taataattgg gccaagttat aatcttttaa atcttgacca
     1261 tatccacctg tatgaattcc cgtcaataca atttccttat atcctgaatt cactagttgc
     1321 gtcgcttgtt caactacttt ttccggatct cttgaacgca ttaagccacg agcccatgga
     1381 ataatacaga atgtgcaaaa gttgttacaa ccttcttgaa tttttaatga cgcacgtgtt
     1441 ctatctgtaa aataagggac atctaattct tcatatttac gatttttcat gatatttcca
     1501 acaccattaa ttggttggcg ttctttacgg aattcatcaa tgtaacctaa tagtttatgt
     1561 ctatcttgtg taccaactac tacatcgaca ccaggaattt ccataatttc agctgatgaa
     1621 gtttgcgcat aacaacctgt tacacagatt acagcatcag gattttgtct tattgcacgt
     1681 ctaattattt gacgactttt tttatcacct gtattcgtta ctgtacaagt attaataaca
     1741 aatacatcag cattcgcttc aaagtcaacg cgctcatagt ttgcttcttt aaataattgc
     1801 cagattgctt cagtttcata atggtttact ttacaaccta atgtgtgaaa cgcaactgtt
     1861 gacataaata ttcaccccat taattctttt tcataactta ttgcacttaa cgcatacaat
     1921 ggcgcagttt ctgcccgtaa aatcctcggt ccaagaccaa caactgtact agtattacta
     1981 aataatgaaa tttcattttc tgacaaacca ccctcaggac caaaaatcat caacacttta
     2041 tcctgagcct tgaattgttg taaagtttgc ttgaaattgc ttaactcacc atcttttgct
     2101 tcctcttcat atgcaataag aatatagtca taattatcaa tagtatcaca aattaatttt
     2161 aaattcgact cgaattgaat agatggaatc actaaacgat agctttgttc agcagcttct
     2221 ttaattattt tttgccaacg ctctatcttt ttggcaactt tcgcctcgtt taatttaaca
     2281 attgagcgtt ccatgctaac agctataaat gatgaagcac ccaattcagt agctttttga
     2341 agcaaccact catatttgtc agctttaatt agcccactgc aaatcgtaac atcaactggc
     2401 aattctgtat taatattttg tttttctttt aaatcaactg caatttgatc acttgttata
     2461 tcagcaattt cacataaata aactgtttga tcattaaaag ttaaaataat tttactacca
     2521 acatcatatc tcattacatt tgttatatga tgaatatctt ctttttttgt aataaaaaaa
     2581 cgctgactta catcagcgtt ttggtctatg aaataacgtt gcacattatt cactcacttt
     2641 ctggccaaca agacaaaccc aaccgttgtc atgttgttct gaaataattt taaaacctac
     2701 acgctccata tgtgactgta taccttcata cttctctttt ataataccag aagtaataaa
     2761 atatccgcct tcatttagag tattataagc atcttcaatc atttcatcaa taatatgcgc
     2821 taaaatattt gctattacaa tatcaaattt ttctgtttcg tctttcaata agttacctgg
     2881 aacagcttca attaacgttt cacaatgatt tcttctgaag ttttctttag ctacactcac
     2941 tgccatttca tcaatatcca acgctttaat acgttttaca ccgattagat gacttgcaat
     3001 acttaatata cctgagccag taccaacatc aattactgaa tgctgtggca atacatatgt
     3061 ttctattgcc ttcaaacaca tacttgtagt cggatgatcg cctgttccaa aagccatacc
     3121 tgggtcgagc tcaatgcaaa gctcttcatc cgcttcttta gcatatgttt cccaactagg
     3181 gactattgtg aacttcttcg acgctcggaa tggatggaaa tagtttttcc attcattttc
     3241 ccaatccgtt tctgcaatga tttgctcact gaattgaacg ttatgttgat caagttcatc
     3301 taaatttaat aactcatctt taattcgctg tcgcaactta tcatcataag tcatctcatt
     3361 aaaataggct ttcaatctta ctcccttatc tggataatcc tcttttttca aagcgtaaat
     3421 ttcaccgtat ttatcttctg gttggttaat taaatcatct gaatcttcta tcacgacacc
     3481 atttgatcca tgattttcaa gtatattggt agccaattct actgcttcat gattaataat
     3541 aattgaaagc tctgtccagt tcatacttta ttctccttta aagaatcttt ttgctctatc
     3601 tttaaaattc gaaggttgtt cattaatttc ttcaccattt aattgggcaa attctttcat
     3661 tagttctttt tgtctatctg ttaatttagt aggcgttact actttaatat caacatataa
     3721 atctccgtat ccatagccat gaacattttt tatacccttt tcttttaagc ggaattgctt
     3781 acctgtttgt gtaccagcag ggattgttaa cataacttca ttatttaatg ttggtatttt
     3841 tatttcatct cctaaagctg cttgtgggaa gctaacattt aatttgtaat aaatatcatc
     3901 accatcacgt ttaaatgttt cagatggttt aactctaaat actacatata aatcaccagc
     3961 aggtcctcca ttcacgcctg gagagccttc accagctaat ctaatttgtt gttcattgtc
     4021 gacaccttca ggtactttca cttctaattt aactgtttta ttttcagtac cttttccgtg
     4081 acatgttgga caagcttctt caaattcttg accacttcca ttacatttag gacaaacttg
     4141 ttcagtacga actctaccta aaattgtatt ttgttctaca gctacatgac cagcgccatt
     4201 acaataacta caagtctttt tacttgttcc aggctttgca ccatcaccat gacatgtttc
     4261 gcatgttaca tctttacgga ttgaaatttc ttttgttgta ccaaataccg cttcttcaaa
     4321 tgtcaatgtc attgtatact gaagatcatc acctttttgc ggtgcatttg gatctctttg
     4381 tctgccgcca ccgaagaaag agctaaagat atcttcaaaa ccgccgccac cgaagccact
     4441 aaaaccgcca aagtcagagc cattgaatcc ttgtccacca aaaccttgtg gaccgtcatg
     4501 tccaaattga tcatagcttg cgcgtttatt atcatcactt aaaacttcat aggcttcaga
     4561 aatttcttta aacttttcat ctgcaccttc ttctttgtta atatctggat gatatttttt
     4621 cgaaagcttt cgatacgctt ttttgatttc atcttttgaa gcatctttac taatgcctaa
     4681 aacttcataa tagtctcttt tggccacagc tatctctcct tttcttaatt aactc
//
LOCUS       DAHHRR010000154         4683 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.156, whole genome shotgun sequence.
ACCESSION   DAHHRR010000154 DAHHRR010000000
VERSION     DAHHRR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.156"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            163..756
                     /locus_tag="NBI42_002419"
     CDS             163..756
                     /locus_tag="NBI42_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCD1778805.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDIINMK
                     PNAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEEIIMAFEHIT
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            740..1891
                     /locus_tag="NBI42_002420"
     CDS             740..1891
                     /locus_tag="NBI42_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HCD1778806.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLDDVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHVMYTHSLAKDDIYAVAYSFEKAESINSTYEHQT
                     SYVSKHHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLKDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKTNTDLIQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGAGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            1891..2499
                     /locus_tag="NBI42_002421"
     CDS             1891..2499
                     /locus_tag="NBI42_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HCD1778807.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYNSGQYRLKIVLNAESEFETIVSPLPEKSSFTAKFQVLPKTVNPTFINNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEEGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            2565..2771
                     /locus_tag="NBI42_002422"
     CDS             2565..2771
                     /locus_tag="NBI42_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778808.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            2869..3579
                     /locus_tag="NBI42_002423"
     CDS             2869..3579
                     /locus_tag="NBI42_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HCD1778809.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            3563..4567
                     /locus_tag="NBI42_002424"
     CDS             3563..4567
                     /locus_tag="NBI42_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCD1778810.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTDR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
BASE COUNT         1726 a          651 c          831 g         1475 t
ORIGIN      
        1 gagggtttca gctaggaacc tctgatattc atttatgtac gatgaataga atataccata
       61 tattagttaa aatcaaggtc agtataatga caatgcttat aaagttttag ctacatccaa
      121 tacatattat aaaaataaaa cacatttaga acggagtata caatgattct agtcatagat
      181 aataatgatt catttacata taatttaata gactatatta agactcaaac gaaactaaca
      241 gttcaagttg ttggtattga taatctgctg atagaagaca tcattaatat gaagccaaat
      301 gcaattgtta tttcacctgg gccgggtaat ccggatgatt atcctatctt gaatgaagtg
      361 ttagaacaat tttatcagcg tgtacctatc ctaggtgtat gtttaggatt tcaatgtatc
      421 gtgtcttatt ttggtggaaa tatcattcac ggctatcatc ctgtacacgg acatactaca
      481 cagttacgtc ataccaatga aggtattttt caaggactgc ctcaaaattt caatgtaatg
      541 cgttatcatt cattaattgc tgacggagcg acttttccaa attgcttaaa gattacagca
      601 aaaaacgatg aagagattat tatggcattt gagcatatta catttccggt ttttggtgtg
      661 caatatcatc ctgaatctat tttgagtgaa tacggttatc gacaagttga attattttta
      721 tcgaaggtag gtgattactg tgagaataga atataattat cgctactatt taactgaaaa
      781 tgaatataag caataccata ttcaattaaa gggatttata aagaagtatg ttgccactaa
      841 gttggatgat gtgggagaag tgatacattt tgcacaagcg cagcaacgac aaggtagata
      901 tgtttcgtta tatttaagtt acgaagcggc aaagtatttt aatcatgtta tgtatacaca
      961 ttcattagct aaagatgata tttatgcagt agcttatagt tttgaaaaag cggaaagcat
     1021 aaattcaaca tatgaacatc aaacttctta tgtatcaaag catcattttt catttgttga
     1081 atcttctgag gttatgatga ctaatattaa acgtgtccaa caagcaattg ttgaaggcga
     1141 aacgtatcaa gtgaactata cggcgcgctt aaaggataac atttattatc ctattagtac
     1201 tttatatgaa cgattaactc aatttagtaa tggtaattat actgcgttat tacaaacaga
     1261 tgaaatccaa gtagcgtcta tctcaccaga attatttttt caaaaaggac agtttaacaa
     1321 tgtcgataac gttatcataa gcaaaccgat gaaagggaca atgcctagag gtaaaacgga
     1381 agctgaagat caacagtatt ataaaacatt gcaaacttct tcgaaagatc gtgcagaaaa
     1441 tgtcatgatt gttgatttac taagaaacga tatagggaga atatcacaga gtggctcaat
     1501 taaggtgtat aaactatttt tcattgaggc atataaaact gtatttcaaa tgacttcgat
     1561 ggtaagtgga actttaaaaa ctaatacaga cttaattcaa attttaacat cgttatttcc
     1621 ttgtggttcg attacaggtg caccgaaact gaatacaatg aaatatatta aacaattaga
     1681 aagttcacct cgtggtatat actgcggagc aattggacta ttacttccaa ctgaagatga
     1741 taaaatgatt tttaatattc cgattcgtac tattgagtat aaatatggac aagcgattta
     1801 tggagccgga gcaggtatta caattgattc taagccaaaa gacgaagtaa atgaatttta
     1861 cgcaaaaacc aagattttgg agatgttata atgcaattat ttgaaacaat gaaaattgat
     1921 aatggacata tccctagact tacttatcat actaatcgca taaaatgttc ttctgagcga
     1981 ttaaacttta aatttgatga acatgcatgg cgaaatgaat taaatgatgt aacaacaaag
     2041 tataacagtg gccaatatag acttaaaatc gtattaaatg ctgaaagcga atttgaaacg
     2101 atagtgtcac ctttacctga gaaaagtagt tttacagcaa aatttcaagt gttgcccaaa
     2161 acagttaatc ccacttttat aaataataaa acgacagaac gaaagcattt agcacacaat
     2221 catgaaacag atttaatatt gctaacttca gaggacggca aggtccttga atttgatatt
     2281 ggcaacattg tcattgaaga ggaaggaaaa tggtacacac caagttataa agatgatttc
     2341 ttaaaaggat gcatgcgtga ttatttaata gatagtgaca aacttgttga aaaagacttt
     2401 aataaaaacg aattgattta taaatatcat aacaatgaga tacgtttatt tttgataaat
     2461 agtttacgag aggttgccga tgtccacctt tgcctttaaa attagtatga taataatgta
     2521 gaaaatgaag tgaaaacaaa tttaaaaatt aaatggggcc aaatatgatt attgtttata
     2581 ttgtgctgtt gttaattctt gtatacgtaa attatcgatt agtgaatcga ttgctatctg
     2641 aaaatagaat atatgttgtt cgtttgatag caacaattac tactgttata agctttatcc
     2701 ttgtatacgc attaattcac gaactcatgc cttttgttgt gcgagcaatg gatttaatgt
     2761 atcaccagta aatttagtga gtaggtaaaa tgaacgtaaa tgaactattc aatcgattct
     2821 ataattaaaa atcaaagtaa agtaaccaaa tattgaagag gtgtacaaat gaaaatatat
     2881 agtcaaggtg accaagccat tgtagtcgca attgaaaaag aagtatctaa aagtttaacc
     2941 gaagatttat taacacttcg ctcatattta attgaacaaa attatccatt tattatagaa
     3001 attgtgccat cagaatcaga catgatgatt gtctatgacg caagggatat gattaaacac
     3061 cataatatac aatcaccttt tttatacatg aaagcactaa tagaatcgat tcatttaaat
     3121 ataaaacatg attttaacca gcaagatttg attgaaatac caattgtgta tggttcgaaa
     3181 tatggtccgg atttagaatc acttttaaaa cattacaaaa tcaagctaga aacatttatt
     3241 gaattacact ctaaggcgca atattttgtt tcgatgatgg gatattcacc tgggtttcct
     3301 tatttaacag gattaaataa gaaattgtat attaatcaca cgagtaaaca gaaaaaattc
     3361 attccagctg gttctgtagt acttgaaggg aaaaaatgcg gtattgtaac aacggataca
     3421 attaatgatt ggttagttat tggttataca ccattatcac tttttaatcc gaaagaatca
     3481 gatttcgcac gcttaaagtt aggcgataat attaaattta gacctatcaa tgaaaatgaa
     3541 ttagaagtag gagcgtttaa agatgtcaat cataattgaa aaaagtggct tattcagtag
     3601 ctttcaggac tttggtagaa ggggatatga acatgatggt gtaattccat gtggtgcact
     3661 tgatacttta gcacatgaaa ttgctaatcg attagttgca aatgacaaga atgaagcaac
     3721 tttggaaatg actaataaaa tggcaacgat tcgttttaca gaacctacgc tgattgcatt
     3781 agcaggaggt aatgtcaaag cttacactga gcatatgact atatctccat ataaattgta
     3841 tttgttagat aaaggcgatg ttttaaaatt tagagaaaca agttatacat cgcgagtgta
     3901 tttagctgtg ggaggcggat ttgaattaga tgcatggtta ggatctaatt caaccgactt
     3961 taatgtaaaa attggtggtt ttaaaggtag aacattacaa gatggcgatg aaataaagct
     4021 taagagagat tatacagatc gtcatcataa gttatttgaa aaccttgctc acacgaaaca
     4081 aacagattgg ggtattgatg gatacgcctt gtcatttaat tatatgtctg atgtatttca
     4141 tgtcgttaaa aataaaggta cggaagattt taaagaagat gccattcaaa gatttgtgaa
     4201 acatgattat aaagtaacga gcaaagcaaa tcgcatgggg atgatgcttg aaggtgaaaa
     4261 aatcaaagct ttttatgaag atatgccacc gtatcagact gtcaaaaaag gaacgataca
     4321 aattaagcgt gatggcacac ctattatttt attaaatgat cattatacgc taggtagcta
     4381 cccgcaaatc ggtacaatcg caagttatca tttaacgaaa ttagcacaaa aaccacaagg
     4441 atcacgtttg aaatttcaat ttatagatat tttaacggct gaaaagaacc ttgttaagta
     4501 tagtaactgg ttaaaccaat tattccatgg aatagaatat agaatgcaat tagaaatgat
     4561 gaaataatat ttggtacgta gctcatacta gagtccgatg caaataattt cctcctaatg
     4621 tataatgaaa taatactgta ttttatctgc gaaatgtatc atattctaat tcgtttcaca
     4681 gta
//
LOCUS       DAHHRR010000155         4558 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.157, whole genome shotgun sequence.
ACCESSION   DAHHRR010000155 DAHHRR010000000
VERSION     DAHHRR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.157"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(48..1118)
                     /gene="dinB"
                     /locus_tag="NBI42_002425"
     CDS             complement(48..1118)
                     /gene="dinB"
                     /locus_tag="NBI42_002425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCD1778811.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASNRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQFIRKDILEQTQLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYRNVHDILMALDIGDFPGVGKASKKVMHDHGIFTGKDLYNKSEFELIHLFG
                     KRGRGLYNKARGIDHSEVKSTRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAEGL
                     NKLQKSAKTVTVKIKTHQFETLSKQMSLRDSVSSENDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTFKNMTIYDFI"
     gene            complement(1365..1895)
                     /locus_tag="NBI42_002426"
     CDS             complement(1365..1895)
                     /locus_tag="NBI42_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCD1778812.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADAYFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(2065..3426)
                     /gene="rlmD"
                     /locus_tag="NBI42_002427"
     CDS             complement(2065..3426)
                     /gene="rlmD"
                     /locus_tag="NBI42_002427"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCD1778813.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYESQLEMKKEQVI
                     NLFQRKAQFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPHAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDVLLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(3507..4454)
                     /locus_tag="NBI42_002428"
     CDS             complement(3507..4454)
                     /locus_tag="NBI42_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCD1778814.1"
                     /translation="MRKRARIIYNPTSGKEQFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
BASE COUNT         1435 a          840 c          620 g         1663 t
ORIGIN      
        1 cctgtaaaat tgacactttt ttaacgacaa ttttacaggg cgtttgttta aataaaatca
       61 tatattgtca tatttttaaa tgttgattgt tcaaggttac ctactgtaac accaataaga
      121 cgtattggta catctgggtc ttttaaatca ttataaagta aatatgcaat gttatagata
      181 tcattttcag aacttacgga gtctctcaaa ctcatttgct tagacaatgt ttcaaattga
      241 tgggttttaa tttttactgt cactgtctta gccgatttct gtaatttatt aagaccttca
      301 gcagttttac ctgataattc ccatactttc ctcaagattt cttcatcatc attaacatca
      361 gtggcaaatg ttcgttcagt acctactgac tttctcactc ttgttgattt tacttcacta
      421 tgatcaattc cacgcgcttt attatataaa cctcgacctc gttttccaaa caaatgaatt
      481 aattcaaatt cacttttatt atataaatct tttccagtaa aaataccatg atcatgcatt
      541 acttttttag atgccttacc aactcccgga aagtctccaa tatctaatgc cattaatata
      601 tcatgaacat ttcgatagtc gattacagtc atgccatcag gtttattcat tccactcgct
      661 aattttgcta aaaatttatt ataagatact cctgcagaag cagttaattg tgtttgttcc
      721 aaaatatctt ttctaataaa ctgtgcaatt ttagatgcag gtaaatctgg acgtacaagt
      781 tctgtaatat cgaggtatgc ttcatcaagg gacatcggct caactttatc tgtataactt
      841 cgaaatatag acattatttg tgctgaagtt tctcggtaag caccaaaatt acttgtgaca
      901 aaatatccat tcggacacaa tttatgtgct tgagacattg gcattgctga atggacacca
      961 tatttacgcg cttcatatga tgctgttgaa acaacgcctc tattacttgc ttttccacca
     1021 actatgacag gtttaccttt taatttggga ttatctctca tttcaacttg tgcaaaaaaa
     1081 taatccatat ctatatgaat aattcgtctc tcagtcaagt gctcacctcc ctactaattt
     1141 ttacttttat aacgcacaaa aatatctcaa cataattata cgctgtgtac gattttttta
     1201 cataaatctt gcacttagcg ataactatat tgagataact acaagttgtt ataaaatcaa
     1261 ttgctattta agcatgatga tgaagacgat tgagtaagaa aacataggta atctgaaata
     1321 attcaagcaa attcattttg ttggtatcat catattaaaa tttattattg agtcggcttt
     1381 tgatgataca aataaatact atcttcatct tgttgaacat atttaaaact actagatatt
     1441 gttaatgcaa caagcagaat atctataaag caataagcgg tatgcatact aaacataaaa
     1501 ataagtgatg aataaccaaa taccttaatt aaaataagca agccagtact taataggatt
     1561 agtggtgaca gcataataat tgagaattgc catttgttga agtaagcatc tgctgtttgg
     1621 aataagattc tgtctttttt tatattaaac ataggtttgc tatctttttt aaataaaaga
     1681 aataatgctc tatggataag ttcatgtaaa atcaataaaa taatgaatcc agcaaaccca
     1741 tatacaagat tgatgatgat attttgatcg acaaccgctg tgacacctaa cgcccactta
     1801 tacgtaaata aaatcacgaa taacgcaata acaagttgca agataataaa ccttcgcatt
     1861 tgaaaattat ttgtcgttaa atcaatttta tgcattgcca cccctcctga tcatgacatt
     1921 cttattcttc tttaaatata gtatacaatg tcacatttaa tttaaaaagt tcatatcaag
     1981 aaagtaaatt ggctgtaata aaattttaat atacgacttc tttcttcact tattaaggcg
     2041 aaattttatc tcaaatcatg tgcgctattt caaattgaat aatgccactg tctcaacatg
     2101 tgttgtttgt ggaaacatat ctaccggtgt tacctcttca agttgatatt tttcagctaa
     2161 taataataca tcacgttgct gtgttgcggg attacatgaa atatagacaa tacgcttagg
     2221 ttctaatgta agcaaagtct gaataaacgt ttcgtcacag ccctttcttg gcggatcaac
     2281 cattacaaca tctggtttaa tcccttgtgc tttccattgt aaaataactt cttcagcttt
     2341 cccacaaaca aaagttgtat tattgcattg gtttatagtc gcattttgtt gtgcgtcttc
     2401 aattgcagaa ggtactactt caacaccgta tacatgtttt gcatgtggtg ccatatatag
     2461 ccctattgtt ccaataccac aataggtatc taatacaact tcattacctg tcaattgcgc
     2521 atactcaatt gctttattat ataatttctc tgtttgttca gaattaattt ggtagaatga
     2581 ctgatcactt attttaaatg tactatctgt taattgatca ataattgtat ctttaccata
     2641 tagcgttata gactgacgtc ccataataac attagagtgg ctatcattaa tgttttgttt
     2701 aatgcttgtc acattaggaa atgcatctaa tatcttctca acaacagcat ttttttgtgg
     2761 ccacttttta ccattagtta caaaaataat catcatttcg tctgtatgat atcctgttct
     2821 tacaaccaaa tgtctcatta aacctttttt caattgttct tgataaatac ttacatttaa
     2881 atcttttaaa atagatttaa cttcattcat cacttcttga tgttgtgaat cttgtattaa
     2941 acaactttcc atgtcaataa tgtcatggct tctttgacga taaaagccca taataacttc
     3001 attctgttca ttcttaccaa ctggaatctg ggacttgttt ctatatctcc aaggatctgt
     3061 catgccaact gtatcgttaa tcttagaatt atcaaactgc gcttttcgct gaaacaaatt
     3121 aatcacttgt tcctttttca tttcaagttg tgattcgtat gataagtgtt gaagttggca
     3181 cccaccacaa cgttcataat atatacatgg cggtgcaaca cgattctcac ttctcgtatt
     3241 tatattaagc aacttaccta tcgcaaagtt cttttttact ttgataattt tatattcaat
     3301 ttgttcatta attaaagctt gtggtatgaa aataggaaag cgatctattt ttacgacacc
     3361 atggccttca tgcgttaaat caacaactgt tcccgtttta atgtcatttt tagctattgc
     3421 ttgcaaaatt ttacctccaa aatgaacagg ttaggaacaa aattatgcgc ttcctaacct
     3481 gccattatat atttcactac ttctgtttat tcttcgatta aattgtcatc aacatgatca
     3541 ttatttatta actcttcatt tacaatatca ttaggtgcaa agacatctat atgacgttct
     3601 agattcaaga aattcgctgg taatttacca ccatattctc catctacatt tagttgtaag
     3661 tctgtgaatg atgaaatatt aattgccttt gctttttcat aaataacttt aggatgctta
     3721 gtatgttctc ctcttgaagc taaagtcata atatgaccaa gttctgcaag gtttgatttt
     3781 tcaactataa ttaacgtaaa atagccgtca tctaacttag cgtccggcac taatttttca
     3841 aatcctgcca ttgaatttgt taaacctaaa aagaataata atgcttctcc ttggaaaaca
     3901 ttaccatcat attcaattct taaatcaaca gctttcattt gtggtaacat ttcgaaacct
     3961 ttgatgtaat aagcaaatgg accaacaata gatttcaatt tactcggtgt ttcataagag
     4021 acttgcgtca attgtccgcc tgcagctaaa ttaataaagt atcgattatt cattttacca
     4081 atatctactt tagtagaatg accttcaatg atgacatcaa gtgcccccat gatgtcatta
     4141 ggtatatgca atgcacgtcc aaagtcatta acagtaccca taggaataac acctagctta
     4201 ggacgattag gcttttctgc gataccatta actacttcat ttaatgttcc gtcaccacct
     4261 gcagcgatta atacatcata attttcatgc atagctcttt ctgcttcaag tgtggcatca
     4321 cctattttct cggttgcata tgcactcgtt tcatatcccg ctttttctaa ttttattaag
     4381 gcatcaggta attctctttt aaattgctct ttacctgatg tcgggttata aatgattcta
     4441 gcacgtttcc tcatatctta tccctctact taaaattcat atattttaac ttcatcttgg
     4501 tttcgtctaa tagggagtga gacagaaata atatttaaca aaatttattt cgttctac
//
LOCUS       DAHHRR010000156         4538 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.159, whole genome shotgun sequence.
ACCESSION   DAHHRR010000156 DAHHRR010000000
VERSION     DAHHRR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.159"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            13..1530
                     /locus_tag="NBI42_002429"
     CDS             13..1530
                     /locus_tag="NBI42_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCD1778815.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIASDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKSHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            1621..1770
                     /gene="rpmG"
                     /locus_tag="NBI42_002430"
     CDS             1621..1770
                     /gene="rpmG"
                     /locus_tag="NBI42_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD1778816.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            2224..2493
                     /gene="rpsN"
                     /locus_tag="NBI42_002431"
     CDS             2224..2493
                     /gene="rpsN"
                     /locus_tag="NBI42_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCD1778817.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            2650..3627
                     /gene="guaC"
                     /locus_tag="NBI42_002432"
     CDS             2650..3627
                     /gene="guaC"
                     /locus_tag="NBI42_002432"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCD1778818.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            3649..>4538
                     /locus_tag="NBI42_002433"
     CDS             3649..>4538
                     /locus_tag="NBI42_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCD1778819.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIQLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYIKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMAKNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYA"
BASE COUNT         1710 a          603 c          854 g         1371 t
ORIGIN      
        1 ggagggatga ctatgtcaca acaagacaaa aagttaactg gtgtttttgg gcatccagta
       61 tcagaccgag aaaatagtat gacagcaggg cctaggggac ctcttttaat gcaagatatt
      121 tactttttag agcaaatgtc tcaatttgat agagaagtaa taccagaacg tcgaatgcat
      181 gccaaaggtt ctggtgcatt tgggacattt actgtaacta aagatataac aaaatatacg
      241 aatgctaaaa tattctctga aataggtaag caaaccgaaa tgtttgcccg tttctctact
      301 gtagcaggag aacgtggtgc tgctgatgcg gagcgtgaca ttcgaggatt tgcgttaaag
      361 ttctacactg aagaaggaaa ctgggattta gtagggaata acacaccagt attcttcttt
      421 agagatccaa aattatttgt tagtttaaat cgtgcggtga aacgagatcc tagaacaaat
      481 atgagagatg cacaaaataa ctgggatttc tggacggggc ttccagaagc tttgcaccaa
      541 gtaacgatct taatgtcaga tagagggatt cctaaagatt tacgtcatat gcatgggttc
      601 ggttcacaca catactctat gtataatgat tctggtgaac gtgtttgggt taaattccat
      661 tttagaactc aacaaggtat tgaaaactta actgatgaag aagctgctga aattatagct
      721 tcagatcgcg attcatctca acgcgattta ttcgaagcca ttgaaaaagg tgattatcca
      781 aaatggacaa tgtatattca agtaatgact gaggaacaag ctaaaagcca taaagataat
      841 ccatttgatt taacaaaagt atggtatcac gatgaatatc ctctaattga agttggagaa
      901 tttgaattaa atagaaatcc agataattac tttatggatg ttgaacaagc tgcgtttgca
      961 ccaactaata ttattccagg attagatttt tctccagaca aaatgctgca agggcgttta
     1021 ttctcatatg gcgatgcgca aagatatcga ttaggagtta atcattggca gattcctgta
     1081 aaccaaccta aaggtgttgg tattgaaaat atttgtcctt ttagtagaga tggtcaaatg
     1141 cgcgtagttg acaataacca aggtggagga acacattatt atccaaataa ccatggtaaa
     1201 tttgattctc aacctgaata taaaaagcca ccattcccaa ctgatggata cggctatgaa
     1261 tataatcaac gtcaagatga tgataattat tttgaacaac caggtaaatt gtttagatta
     1321 caatcagagg acgctaaaga aagaattttt acaaatacag caaatgcaat ggaaggcgta
     1381 acggatgatg ttaaacgacg tcatattcgt cattgttaca aagctgaccc agaatatggt
     1441 aaaggtgttg caaaagcatt aggtattgat ataaattcta ttgatcttga aactgaaaat
     1501 gatgaaacat acgaaaactt tgaaaaataa atttgatatg tagtttctat attgcgtagt
     1561 tgagcagttt atgatatcat aataaatcgt aaagattcct aacaagagag ggtgtttaac
     1621 gtgcgcgtaa acgtaacatt agcatgcaca gaatgtggcg atcgtaacta tatcactact
     1681 aaaaataaac gtaataatcc tgagcgtatt gaaatgaaaa aatattgccc aagattaaac
     1741 aaatatacgt tacatcgtga aactaagtaa ttcttatcat tcaaatacga cgatttgaaa
     1801 ataaagcggg cttacctatt atattgggga gctcgctttt ttatgaaatt tttgtgaaga
     1861 gtgattaatg aattgagttt catcgataga acaatttatg attgtattag ttgttacttt
     1921 attaaaattt gagaatattt atagaaggaa atagattact gattttataa agtcactttg
     1981 ttagcgaatg cttgaaagag tatttaatat agtagaattt aaaatttcaa agtggaattt
     2041 aataagtacg aagtaattgt ggatatgttt tataaatgtt cgacaataca ttttaatgtt
     2101 aaatatgatg gtttagaaaa taatttaaca aagaaatgaa actttactgt tgaattatgt
     2161 gaggattgtg ttattatata aatcgtaata attacgattt gataaaaagt gaggtaacta
     2221 tatatggcta agaaatctaa aatagcaaaa gagagaaaaa gagaagagtt agtaaataaa
     2281 tattacgaat tacgtaaaga gttaaaagca aaaggtgatt acgaagcgtt aagaaaatta
     2341 ccaagagatt catcacctac acgtttaact agaagatgta aagtaactgg aagacctaga
     2401 ggtgtattac gtaaatttga aatgtctcgt attgcgttta gagaacatgc gcacaaagga
     2461 caaattccag gtgttaaaaa atcaagttgg taaaatcaaa tttcgtactt tagcccattt
     2521 acaatatcaa taaaacaatg tacagtatat acgaatgcta taaactgaat gttttctcat
     2581 attaataagg aaacattcgg tttttaattt gcatttaaaa ataattagtt tttaaagggg
     2641 ctatttaaag tgaaaatatt tgattacgaa gatattcaat taatacctaa taaatgcata
     2701 gttgaaagta ggtctgaatg tgatacaact atccaatttg gtccgaaaaa attcaagcta
     2761 cctgtagttc ctgcaaatat gcaaacagtt atgaatgaga aattagcgaa atggtttgct
     2821 gaaaatgatt acttttatat catgcatcgt tttgatgaag aagcaagaat accttttata
     2881 aaacatatgc aaaattcagg cttatttgca tctatttcag ttggtgtaaa gaaagcggaa
     2941 tttgatttta ttgaaaagtt agctcaagaa aaattaatcc ccgaatatat tacaatagat
     3001 attgcgcatg gtcactcaga ttcagtgata aacatgatta aacatataaa aacccatata
     3061 cctgatagtt ttgttattgc tggtaatgtt ggtacgccag aaggtgttag agaattagaa
     3121 aatgctggtg ctgatgctac caaagtcggt ataggtcctg gtagagtttg tattacaaag
     3181 attaaaacag gttttggtac tggtggttgg cagttagcgg cattaaatat atgtagtaaa
     3241 gcagctcgta aacctttgat tgccgatggt ggtataagaa cgcatggcga catcgctaag
     3301 tcaattagat ttggtgcatc aatggtcatg attggttcat tatttgcggc acacgaagaa
     3361 tcacctggtg aaactgtaga acttgatggt aaacagtata aagaatattt tggtagtgca
     3421 tctgaatttc aaaaaggcga acataaaaat gtagaaggta aaaaaatgtt tgtagaacat
     3481 aaggggtcat taatggatac cttaaaagaa atgcaacaag atttacaaag ctcaatttca
     3541 tatgccggtg gaaaagactt gaaatcatta cgtactgtag attatgttat tgttagaaac
     3601 tctattttca acggtgatag agattaatat ttatagtagg tgatgtaaat taaaaaattt
     3661 atagtaactg ttgttgcgtt tttatcaatt attatcattg cgccaataac agaatttaaa
     3721 ccattcattc aattacaaaa tgaagtaaga caatatattg acattcacat caataaagaa
     3781 acaatttctg cggaaaataa attggatacg ccgaagaaac aacaatttgc ctttaacaat
     3841 atacaaatga acatgtcgaa atcagatgtt gagaaaacat taaataaacc aaaaagagtg
     3901 acatttaatg aatatggtac gaagtggtat acgtattatg atgatgatta caataatttt
     3961 ataatgataa gttacattaa agataaagtt aatgcgttat atacaaatca aaatatcatc
     4021 acttcaaaat caaaaattaa atacaataca ccgaaatcgg ttgtaaggca aagattaggc
     4081 gaaccagaaa cagagattgt taaaggtaga gtgcgttacg aacaaaataa taaagaatat
     4141 gatgttttcc ataaaaatca catttatacg acggtatttt atgataagca tcgacgtaat
     4201 aatgtaacag ctgttttaca agtaagtgat gctatggaaa atagattaaa agaacaatat
     4261 ggagcacctt cgaaatcgct tgcagatagt tttgaactac aaaattttga tttagttaat
     4321 gctgaaagaa aacaacatca attatctaca ttgaagtatt ctaaacagaa ttctgaaact
     4381 gctcgtaagc atagtaaaga tatggccaaa aatcattatt ttgatcatac aaatttaaaa
     4441 ggtcaatcac catttgatcg attgaaaaaa gatggtatta catttaactc agccggagag
     4501 aatttagcat atggtcaagt tagtagtatc tatgcaca
//
LOCUS       DAHHRR010000157         4532 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.160, whole genome shotgun sequence.
ACCESSION   DAHHRR010000157 DAHHRR010000000
VERSION     DAHHRR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.160"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            189..344
                     /locus_tag="NBI42_002434"
     CDS             189..344
                     /locus_tag="NBI42_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778820.1"
                     /translation="MMVKKLLKDIKKNFLCEQSNITFKMILFGYKRLSKMIFQFEINH
                     INFLREG"
     gene            352..873
                     /locus_tag="NBI42_002435"
     CDS             352..873
                     /locus_tag="NBI42_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778821.1"
                     /translation="MINIISAIGSIGTFIMALFYFVSVSVQLYQMKISFLPALGFNQI
                     LLEKGGNQLNIMNSATEEHHHKDYIKLYNLGGGAAKKIAIEVLLGNDKVIQKKYVNIL
                     PSKEGYMLPINKNVYEELERTIENNGYEADLNVRMTYYHNVSRKQQEVILKGQIDRFN
                     TYNNKEIYDLQFI"
     gene            1012..1542
                     /locus_tag="NBI42_002436"
     CDS             1012..1542
                     /locus_tag="NBI42_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1778822.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNNLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREARLKGYNFISVLGWPKYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKIGTINYTSAFEKI"
     gene            1805..4495
                     /gene="clfA"
                     /locus_tag="NBI42_002437"
     CDS             1805..4495
                     /gene="clfA"
                     /locus_tag="NBI42_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HCD1778823.1"
                     /translation="MNMKKQEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDPSSVNAAPKTDNTNVSDSNTTTNTNSDETNVAQNPAQQETTQSA
                     STNATTEETPVTGEATTATNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSVN
                     SPQNSTNAENVSTTQDTSTEATPSNNESAPQSKDASNKDVVNQAVNTSAPRMRAFSLA
                     AVAADAPATGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVQGDTFKI
                     TVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLTM
                     PAYIDPENVTKTGNVTLTTGIGTNTDSKTVLIDYEKYGQFHNLSIKGTIDQIDKTNNT
                     YRQTIYVNPSGDNVVLPALTGNLIPNTKSNALIDAKNTDIKVYRVDNANDLSESYYVN
                     TSDFEDVTNQVRISFPNANQYKVEFPTDDDQITTPYIVVVNGHIDPASTGDLALRSTF
                     YGYDSNFIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPGS
                     DSGSDSGSDSNSDSGSDSGSDSDSASDSDSDSGSDSGSDSDSASDSDSASDSDSASDS
                     DSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSNSDSDSDSAS
                     DSDSGSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSASDSDSGSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDS
                     DSSSDSDSGSDSDSDNDYDSESDSNSDSDSGSNNNVVPPNSPKNGTNASNKNEAKDSK
                     EPLPDTGSEGEANTSLIWGLLASLGSLLLFRRKKENKDKK"
BASE COUNT         1659 a          681 c          882 g         1310 t
ORIGIN      
        1 atgtagaatt ttgatttgtt aagaaattac gtttataaaa ataaaaaaat tcaacttatt
       61 tgtatgagat aaatatgtat tgaagaagat gtgttattaa attggaaaat actggtcaaa
      121 gatgggagct cttaaaagcg ttattgtatt ctttagtcaa tgcaaataga ttgccataat
      181 aaacgtactt gatggttaaa aaattactta aggatataaa gaaaaacttt ttatgtgagc
      241 agtcgaatat aacgtttaaa atgattcttt ttggatataa acgattaagt aaaatgattt
      301 ttcagtttga aattaatcat ataaatttct taagggaagg ttgatatctt aatgattaat
      361 attatttcag ctataggatc tattggaaca tttattatgg ctttatttta ttttgtatca
      421 gtttcagttc aactttatca aatgaaaatt agctttctgc cagctttagg ttttaatcaa
      481 attttattag aaaagggggg gaatcaactt aatataatga attcggcaac agaagagcat
      541 catcataaag attatattaa actatataat ttaggtggcg gtgctgctaa aaaaattgca
      601 atagaggttt tattgggtaa tgataaagtc attcagaaaa agtacgtgaa tattttacct
      661 agtaaagaag ggtacatgtt accaattaat aaaaatgtgt acgaagaatt agaaagaacg
      721 attgagaaca atggttatga agctgatttg aatgtacgta tgacttatta tcataatgta
      781 agtcgcaaac aacaggaagt tatattaaaa ggtcaaatcg accgttttaa tacttataat
      841 aataaagaaa tttatgattt gcagtttatc taaaaattga tttaagaggg tagttgttta
      901 ttacgaaaaa tatcattcaa ttttaatgaa ataatggcgt cattactata aaatattact
      961 ttatgttgtt atgcattttt ctataagata gaactaaaag gaggggcaaa gatgcaaatt
     1021 agacaaatac atcaacatga ctttgctcaa gttgaccagt taattagaac ggcatttgaa
     1081 aatagtgaac atggttatgg taatgaatca gaactagtag accaaattcg tctaagtgat
     1141 acgtatgaca ataacttaga attagtagct gttcttcaaa atgaagttgt agggcacggt
     1201 ttactaagtg aagtttatct tgataacgag gcacaacggg aaattggatt agtgttagca
     1261 cctgtatctg ttgatattca tcatcaaaat aaaggtattg ggaagcgatt gattcaagca
     1321 ttagaacgag aagcaagatt aaaaggatat aattttatca gtgtattagg atggccgaag
     1381 tattatgcca atctaggata tcaacgcgca agtatgtacg acatttatcc accatatgat
     1441 ggtataccag atgaagcgtt tttaattaaa gaattaaaag tgaacagttt agcgggaaaa
     1501 ataggtacca taaattacac atctgctttt gaaaaaatat gatttcaagt taggattaca
     1561 ttaggcagag ttcatattaa gaatacaaat tgtttgcaat gaaatcgtgc gttgtcgttt
     1621 gtaattttaa aaatagcaat aaataaaatg tttgttagta aagtattatt gcaggtaata
     1681 aaatatcgat acaaattaat tgccattatg taatttcggt gtataattgc attaatagag
     1741 attaaatata tattaaaagg gtatacagtt aatataaaat gactttttaa aaagagggaa
     1801 taaaatgaat atgaagaaac aagaaaaaca cgcaattcgt aaaaaatcga ttggcgtggc
     1861 ttcagtgctt gtaggtacgt taatcggttt tggactactc agcagtaaag aagcagatgc
     1921 aagtgaaaat agtgttacgc aatccgatag cgcaagtaac gaaagcaaaa gtaatgatcc
     1981 aagtagcgtt aatgctgcac ctaaaacaga caacacaaac gtgagtgatt ctaatacaac
     2041 gacaaacact aatagtgacg aaacgaatgt agcgcaaaat ccagcacaac aggaaacgac
     2101 acaatcagca tcaacaaatg caactacgga agaaacaccg gtaactggtg aagctactac
     2161 ggcaacgaat caagctaata caccggcaac aactcaatca agcaatacaa atgcggagga
     2221 attagtgaat caaacaagta atgaaacgac ttctaatgat actaatacag tatcatctgt
     2281 aaattcacct caaaattcta caaatgcgga aaatgtttca acaacgcaag atacttcaac
     2341 tgaagcaaca ccttcaaaca atgaatcagc tccacagagt aaagatgcaa gtaataaaga
     2401 tgtagttaat caagcggtta atacaagtgc gcctagaatg agagcattta gtttagcggc
     2461 tgtagctgca gatgcaccgg ctactggcac agatattacg aatcagttga cgaatgtgac
     2521 agttggtatt gactctggta cgactgtgta tccgcaccaa gcaggttatg tcaaactgaa
     2581 ttatggtttt tcagtgccta attctgcggt tcaaggtgac acattcaaaa taactgtgcc
     2641 caaagaatta aacttaaatg gtgtaacttc aactgctaaa gtgccaccaa ttatggccgg
     2701 agatcaagta ttggcaaatg gtgtaatcga tagtgatggt aatgttattt atacatttac
     2761 agactatgta aatactaaag atgatgtaaa agcaactttg accatgcccg cttatattga
     2821 ccctgaaaat gttacaaaga caggtaatgt aacattgaca actggcatag gaaccaatac
     2881 tgatagtaag acagttttaa tcgactatga gaaatatgga caattccata atttatcaat
     2941 taaaggtacg attgatcaaa tcgataaaac aaataatacg tatcgtcaaa caatttatgt
     3001 caatccaagc ggagataacg ttgtgttacc tgccttaaca ggtaatttaa ttcctaatac
     3061 aaagagtaat gcgttaatag atgcaaaaaa cactgatatt aaagtttata gagtagataa
     3121 tgctaatgat ttatctgaaa gttattatgt gaatactagc gattttgaag atgtaactaa
     3181 tcaagttaga atttcatttc caaatgctaa tcaatacaaa gtagaatttc ctacggacga
     3241 tgatcaaatt acaacaccgt atattgtagt tgttaatggc catattgatc ctgctagtac
     3301 aggtgattta gcactacgtt cgacatttta tggttatgat tctaatttta tatggagatc
     3361 tatgtcatgg gacaacgaag tagcatttaa taacggatca ggttctggtg acggtatcga
     3421 taaacctgtt gttcctgaac aacctgatga gcctggtgaa attgaaccaa ttccagagga
     3481 ttcagattct gacccaggtt cagatagcgg ttcagattct ggcagcgatt ctaattcaga
     3541 tagcggttca gattcgggta gtgattcaga ttcagcaagc gattccgatt cagatagcgg
     3601 ttcagattcg ggtagtgatt cagattcagc aagtgattcc gattcagcga gtgattcaga
     3661 ttcagcgagt gattcagatt cagcaagtga ttcagattca gcgagtgatt cagattcagc
     3721 aagcgattca gattcagcaa gcgattcaga ttcagcgagt gattcagatt cagcaagcga
     3781 ttccgactca gacagcgatt cagattctga cagtgactca aactcagata gcgattcaga
     3841 ttcagcgagt gattccgact caggtagtga ctcagactca gacagtgact cagattcaga
     3901 tagcgattca gactcagaca gcgattcaga ttcagacagt gactcagact cagacagcga
     3961 ttcagactca gacagcgatt cagattcaga cagtgactca gactcagata gtgactcaga
     4021 ctcagatagc gattcagatt cagcgagtga ttccgactca ggtagtgact cagattcaga
     4081 cagtgactcg gattcagaca gcgattcaga ctcagacagt gactcagaat cagatagtga
     4141 ttcagattcc gacagcgact cagattcagc gagtgattca gactcaggta gtgactcaga
     4201 ttcatcaagt gattccgact caggtagtga ctcagattca gacaatgact acgactcaga
     4261 aagtgattca aatagcgatt ccgactcagg ttctaacaat aatgtagttc cgcctaattc
     4321 acctaaaaat ggtactaatg cttctaataa aaatgaggct aaagatagta aagagccatt
     4381 accagataca ggttctgaag gtgaagcgaa tacgtcacta atttggggat tattagcatc
     4441 attaggttca ttactacttt tcagaagaaa aaaagaaaat aaagataaga aataagtaat
     4501 aatgatatta aattaattat atgattcatg aa
//
LOCUS       DAHHRR010000158         4448 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.161, whole genome shotgun sequence.
ACCESSION   DAHHRR010000158 DAHHRR010000000
VERSION     DAHHRR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.161"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..1221)
                     /locus_tag="NBI42_002438"
     CDS             complement(<1..1221)
                     /locus_tag="NBI42_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1778824.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEEVITRLKEVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFDTKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVKQLK"
     gene            complement(1240..2478)
                     /gene="hflX"
                     /locus_tag="NBI42_002439"
     CDS             complement(1240..2478)
                     /gene="hflX"
                     /locus_tag="NBI42_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCD1778825.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLQTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDEAIETNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            2590..3066
                     /locus_tag="NBI42_002440"
     CDS             2590..3066
                     /locus_tag="NBI42_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCD1778826.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(3288..3521)
                     /gene="hfq"
                     /locus_tag="NBI42_002441"
     CDS             complement(3288..3521)
                     /gene="hfq"
                     /locus_tag="NBI42_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCD1778827.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETESQESTESEE"
     gene            complement(3536..>4448)
                     /gene="miaA"
                     /locus_tag="NBI42_002442"
     CDS             complement(3536..>4448)
                     /gene="miaA"
                     /locus_tag="NBI42_002442"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCD1778828.1"
                     /translation="VVIVGPTASGKTELSIELAKRINGEIISGDSMQVYKHMNIGTAK
                     VTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAGGTGLYIQS
                     LIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAEFDAVSAENIHPNNRQRV
                     LRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVNIMLDHGLF
                     REVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYAKRQMTWFK
                     NKMSVHWLDKENMSLQMMLDEITTQIK"
BASE COUNT         1364 a          827 c          632 g         1625 t
ORIGIN      
        1 tttcaactgc ttaacagctc ttgtcacagc aattttaaca tgttcatatg ttaaacctcc
       61 ttggacatac gcttcataag gaggtcgaat tggaccatca gcagacaatt caattgaaga
      121 gccttgaata aatgtaccag ccgccataat aacatcatct tcataacctg gcatataact
      181 aggttctgga ctaaaatgcg cattgattgg tgatgcatgt tgaatactct gacaaaatga
      241 aatcatttgt tcctttgtat caaatttaac tgtttgaatt aaatcagttc gtttttcgta
      301 gtacttcggc gttgtgttca tattcatttt ttctaaaaat aaactagtaa acaatgcacc
      361 tttaagactc tgactgacaa cgtgtggtgc taaaaagaaa ccttgataca tttcaagcaa
      421 tgcatttaat gatgcacccg cttctttacc aataccaggt gctgtcaatc tataaccaca
      481 tcgttcaatt aaatcttttc taccagcaat gtatccacca atcttagcta aaccaccgcc
      541 aggattttta attaatgatc ctgctattaa atcggcacca cattctatag gttcacgtct
      601 ttcaacaaat tccccataac agttatccac aaatattaaa atattaggat gcacttcttt
      661 caacctagta atcacctctt caatttcatc tagaggaatt gaaggtcttt gatcatagcc
      721 tttcgaacgt tgaatcgcta ttactttggt gcgctcagaa accccatcta acacactttc
      781 aatatcgatc ttaccttctt taagtgctat atctttatac gatacgccgt gctccattaa
      841 actttcaata ccatttccgt ttacaccaat gacttcaagt aaagtgtcat atggacttcc
      901 tgttatataa attaattcat caccatgttt taataaactt tgtaatgcaa tagtaatcgc
      961 atgcgtacct gaaataattt gcggacgaac aattgcatct tctgctttaa atgcctgcgc
     1021 atatatttct tctaaatgat cacgtccaaa atcatcatac ccgtatcctg ttgatccttg
     1081 tagatcactt tcggttgcct tgacatgatg aaatgcattt aatacttttt cttgattaat
     1141 atatgcagtt tcttcaattt ctttaaaata tggtgctaac gttgattcga cgtcagctac
     1201 tatcttactt atatctttca tttgaatttc gtccttttat tattttttaa atccttttat
     1261 acgataaaca ttttcaattt catcatattt aagttcagtc actaaagtat gttgttttag
     1321 aaaatataat cgatctgcat tattcgtctc aattgcctca tcataataag ttaattgtct
     1381 tttgatttca tcaataaata acgtcttaac aagtaattta tcaccatcat ttttagaaga
     1441 aacaaaaaca ttaggcaaat cacttgctgg acgatttgat gcatgatcac ataagtcctt
     1501 tttattaaaa ataactattt gagaaatatg actcatatct aattgtttga ttaaatcatt
     1561 aactgtgtca tactgcgtac gatattcagg atggctacta tctacgacat gtactaataa
     1621 atctgcacct ttagcctctt ctaaagttga tttaaaagct gcaattaacg tcgtaggtag
     1681 tttctgtata aaaccaacag tatctgaaat aattaaatta aatccatcat taatttgaat
     1741 ttgtcgtgtt ttaggatcta acgttgcaaa taattgatct ttttcatatg tctcttcatt
     1801 tgctaaaaca ttgaaccatg atgatttacc agcatttgta taaccaacta aagccacttg
     1861 aaacacctga ttttgatttc ttttatttcg atatctttcg cgatgttcct caactgtttg
     1921 taattgatgt ttaatttcat tcatacgagt cctaatatgt cttcggtcca tctctaattt
     1981 cgtttcacca ggacctctag taccaattcc cccacctaaa cgcgacaaac ttttaccatg
     2041 tccttgcaat ctaggtaata aataatcaag ttgtgcgagt tctacttgca acttaccttc
     2101 tttgcttcgt gctcttaatg caaatatttc aagaatcaac tgagttctat caattatttt
     2161 tacacctaaa gcttcattta gtgatttgga ttgtgcagtc gttaattcat catttgtgat
     2221 gactacatca atatctttga actcaataaa tgcttgaatt tcttcaattt tacctttacc
     2281 aacataatat ttgcgatcta cccgatctct gttttgagta atttgaccca acacttcaag
     2341 ttggcaagtc tctgataaag atgataattc ctccattgta gactcaaaat taaattgctt
     2401 atcatcttga gcatgtacac cgactaagac agctttttct agtttatttt tagtatcatg
     2461 aatttgttgc tgagccatgt tatacatctc cttaataaaa tcctactcaa agtattctat
     2521 catagagttt tagtaaagac tattacacga ttttatggta cattttaagt aaatcgaaag
     2581 gatgattcaa tggagacaat ttatgatttt gtagtagaaa caaataaagg tgttacttac
     2641 aaattagatg catataaggg tgacgttatg ctaattgtca atacagctag cgaatgtggc
     2701 tttacttcac aatttgaagg tttgcaatca ttatacgaga aatacaaaga tcaagggttt
     2761 gtaattctag gtttcccttg taatcaattt ggtggccaag aacctggatc aggcgaagaa
     2821 gcagctcaaa actgtaaatt aaactatggt gttaccttcc ctatgcatca aaaaatagac
     2881 gtaaaagggg aacatcaatt acctttattt agatatttga ctgcggcaca acacggattc
     2941 tttaatgaga aaatcaaatg gaatttcact aagtttttag tagatcgtga aggcaacgtt
     3001 gttaaacgat ttgcacctca aaaaaaacct gttcaaatcg aaagagaaat agaaaagtta
     3061 ttataaaata tactagctca tagcaaataa aacgcttcat ataaagtcgt atcatatatt
     3121 gactcgacct ttatatgaag cgtttttatt acaaacattc ctctattgta tcttgtcatt
     3181 tgaacatcat ttaattgatg tcacatatga ctttatttct atatctattt atcatacttc
     3241 aaacgttatg tacacgagtc attaaatcgt gcccatatga ttacaactta ttcttcactt
     3301 tcagtagatt cttgactttc agtttctact gtataagtgc tgatcgcatg tttgtaaatc
     3361 aagtgttgtt tgccttgaga atttaagctt acgacatact tgtcgtattc ttcaataaca
     3421 cctttcattt ggaaaccgtt tagaaagaat acagttactt cagtttggtt tgctttaaaa
     3481 ttctctagtg ctttgtcttg gatgttttcg tttgcaatca tctgtcggac tccttttact
     3541 taatctgggt tgtaatctca tctaacatca tttgaagtga catattttct ttatctaacc
     3601 aatgaacact cattttattc ttgaaccatg tcatttgtcg ttttgcatat tggcgtgaat
     3661 gttgctttaa atcattgaca gcatcttcat aaatcatttg tccgttaatc acaggtatta
     3721 attctttata tccaatagct tgcatacttt ggcaagattc atagccttgt tcaacaagtt
     3781 gttgcacttc tctaaataat ccgtgatcca acataatatt aacacgttta tttattcttg
     3841 aatataatgt tttacgcgac atttcaatcc ctaataataa tgtatcataa ttttcagtaa
     3901 attgttgcac tttcttgcga ttacttaaaa gtttttttgt ttttaaataa tattcaatag
     3961 cgcgcaacac tctttggcgg ttgttagggt gaatattttc tgcagaaacc gcatcaaatt
     4021 cagctaaata atcgtgtagt tgctgattat ctaaatgttc taatgcagat aacttttgtt
     4081 taactatgga taattgtgca ggtgtaactg tttcatcttc taattcataa ttatatatta
     4141 atgattgaat atataagcct gttccacctg ctatgattgg aactttacct ctattcgtta
     4201 tatcagtaat taaatcttct gctaatcgct tgaattcata tgctgaaaat gtatcatcag
     4261 gattcaagat atcaattaaa tgatgtggaa taccatccat ttcttcaggt gttacttttg
     4321 cagttccaat attcatatgt ttgtagactt gcatagagtc accgcttatg atttcaccat
     4381 tgatacgctt cgcgagttct atgctaagct ctgttttacc tgaagcagtt ggccccacaa
     4441 ttactaca
//
LOCUS       DAHHRR010000159         4409 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.162, whole genome shotgun sequence.
ACCESSION   DAHHRR010000159 DAHHRR010000000
VERSION     DAHHRR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.162"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            95..1228
                     /locus_tag="NBI42_002443"
     CDS             95..1228
                     /locus_tag="NBI42_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCD1778829.1"
                     /translation="MINEQRLLNTFLELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMKSTIEEGEVPKLYLTSHMDTVVPAINVRPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNETVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARQSK"
     gene            1296..2702
                     /gene="gndA"
                     /locus_tag="NBI42_002444"
     CDS             1296..2702
                     /gene="gndA"
                     /locus_tag="NBI42_002444"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCD1778830.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            3355..3507
                     /gene="stsA"
                     /locus_tag="NBI42_002445"
     CDS             3355..3507
                     /gene="stsA"
                     /locus_tag="NBI42_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stapholysin S"
                     /protein_id="HCD1778831.1"
                     /translation="MMKINNHTINGYSDISSSEAMQYAAGCCSCSCSCSCSCSCSCTS
                     ASTAEQ"
     gene            3567..>4409
                     /locus_tag="NBI42_002446"
     CDS             3567..>4409
                     /locus_tag="NBI42_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1778832.1"
                     /translation="MESLMTIKNLKVDINNRKILNLNNSTINIYSKDKIALIGKNGAG
                     KTTLINCLLNEIKYSGVIDRKIKSKDIGIVFQENKYGDLIKVHELIYLITAYSKRSKQ
                     FKAFTKAFSLEHLINKFVKDLSLGEQKRLTVGLVLNEQKKLYIFDELTSGLDYEKRQN
                     LLQKTKEITQNKAVIKITHYFDEVENWANKLIIIDKGELLYYGSIDQFFSKFYHYALI
                     EIENQELDYQVRVVIEKINADVYFNTKIVTHNKEQMNDVINILNERKIEFKINMQNIY
                     STYLI"
BASE COUNT         1629 a          570 c          823 g         1387 t
ORIGIN      
        1 gataaatctg tgtaacgtca attactgtaa cattttattt catcaatttt taccgtttgg
       61 cgtacttgga aattttgata aaaggggttc cattatgatt aatgagcaaa gattacttaa
      121 tacgttttta gaacttgtac aaatcgattc tgagacaggg aatgaatcaa caatccaacc
      181 tattttaaaa gaaaaattca tagcattagg gttagatgtt aaagaagatg aagcggctaa
      241 acatccgaaa ttaggtgcca ataatttagt gtgtacaatg aaaagtacta tcgaagaagg
      301 cgaagtacct aaattatatt tgacgagcca tatggatact gttgtacccg caattaatgt
      361 aaggccaatt gtaaaagacg acggctacat atactctgat ggtacgacta ttctaggtgc
      421 agatgataaa gcaggattag cagcgatgct tgaagttttg caagtgataa aggaacaaca
      481 aatcccacat ggacaaattc aatttgtgat tactgttgga gaagaatcag gtttaattgg
      541 tgctaaagaa ttaaattcag agttgttgga cgcggacttt ggttatgcta ttgatgcaag
      601 tgctgatgtc ggtactacgg ttgtaggtgc accgacgcaa atgttaattt cagctaaaat
      661 ttttggcaaa acggctcatg caagtacgcc aaaagagggt gttagtgcta ttaatattgc
      721 ggcgaaagca attagtcgaa tgaaattagg acaagttgat gaaataacta ctgccaatat
      781 tggtaagttt catggcggtt cagctacgaa tattgttgca gatgaagtca ttttagaagc
      841 agaggcacgt tctcacgatc cagaaagaat aaaaacacaa gtcaaacata tgactgatgt
      901 gtttgaaaca actgcatcag aattaggtgg gaaagcagta gttacagtgg agcaaagtta
      961 tccaggattt aaaattaatg ataatgaaac ggttgttaaa attgcacaag aaagtgctcg
     1021 taatttagga ttatctgcaa atacaattat ttctggtggc ggttcagacg gcagtattat
     1081 taatacattt ggtattcctt cagtcatttt aggagtgggt tatgaaaaaa tccatacaac
     1141 aaatgagaga atgccgatta aatcattgaa cttgcttgca agtcaagtgt tagaaataat
     1201 caaaatagtt gcgcgacaat cgaaatgatt tagtgtagtg tatgtctatt ttgtgataca
     1261 atactaatga aaaatcaaca agtgaggtaa gtaatatgac acaacaaatt ggagttattg
     1321 gtttagccgt tatgggtaaa aacctagctt ggaatattga atcacgcgga tatagtgtat
     1381 ctgtatttaa ccgctcaagt gaaaaaactg atttaatggt tgaagaatca aaagggaaaa
     1441 atattcatcc aacgtattca ttggaagaat ttgttaattc tttagaaaaa ccacgtaaaa
     1501 ttttattaat ggttcaagca ggtaaagcta cggatgcaac aattgatagt ttgttacctt
     1561 tattagatga tggagatatt ttaattgatg gtggtaatac aaactatcaa gatactatca
     1621 gacgtaataa agcattagca caaagtgcaa tcaactttat tggtatgggc gtttctggtg
     1681 gtgaaattgg agcgttaact ggtccgtcat taatgccggg tggacaagaa gaagcttata
     1741 acaaagttgc agatattctt gatgcaattg ctgcaaaggc aaaagacggt gcttcatgtg
     1801 ttacttatat tggaccaaat ggtgctggac actatgtgaa aatggtacat aatggtattg
     1861 aatatgctga catgcaatta attgcagaaa gttatgctat gatgaaagaa ttattaggca
     1921 tgtctcatga agacattgct caaacgttca aagactggaa tgctggtgaa ttagaaagtt
     1981 atttaattga aatcactggt gatattttca tgaaattaga tgaaaataaa gaagcattag
     2041 ttgaaaaaat tcttgatact gcaggtcaaa aaggtacagg aaaatggact tcaattaacg
     2101 cattagaatt aggtattcca ttaaccatta ttactgaatc tgtatttgca cgttttatct
     2161 cttcaatcaa agaagagcgc gtaaatgctt ctaaagaatt aaatggtcca aaagcatcat
     2221 ttgatggtga caagaaagac tttttagaaa aaattcgtaa agcattatat atgagtaaaa
     2281 tttgttctta tgcacaaggt ttcgcgcaaa tgcgtaaagc aagtgaagat aatgaatgga
     2341 atttaaaact tggtgattta gctatgattt ggagagaagg ttgcattatc cgtgcacaat
     2401 tcttacaaaa aattaaagat gcatatgata ataacccagg attacaaaac ttgttattag
     2461 atccttactt taaaaacatc gttactgaat accaagatgc attgcgcgac gttgtagcaa
     2521 caggtgttca aaatggtgta ccaacacctg gattctcatc aagtattaac tactatgata
     2581 gttacagagc tgctgattta ccagcaaact taattcaagc tcaacgtgac tacttcggtg
     2641 cacatactta tgaaagaaaa gataaagaag gcgtattcca tacacaatgg attgaagaat
     2701 aatattgatt aaaggatagt tgaaagatac tatccttttt tattatttaa atagaagtta
     2761 agtattaaaa tctaataaat ataatttttg atgatggatc acttcctagg atgacgtttt
     2821 agctatccca accggcacat tattgtaagc tgactttccg ccaatttctg tactggggca
     2881 attatcttcg acgggcactg ctcactcagg attatcacta ttaggtgctt aatataagga
     2941 aaatagtggc tcaactagct gtatggtatt aaattcattt caaaaagctg atttctatca
     3001 attaattaac agaaatcagc ttttttactt tgtctaagaa cattatgtcc cagactcact
     3061 gctttggttt tgttaatcaa taaagctaca ttaggatttt atacctttaa atttatcaaa
     3121 atatataaac atattttcat tatttgaaaa ttgtgtatta taaagagaaa ataatgaact
     3181 tttttagtta tacttcactt aatttaatgc tatatcttta ttattcgtta aatctataac
     3241 tttcatacta aaaaataatt aaaataaaag attgactaaa gatatattgc gtgatatatt
     3301 tctgtttata aatagtaatt ttacatcatg tttcttaggg tgaaggaggg cgaaatgatg
     3361 aaaattaata accatactat taatggatat tcagacatta gtagttctga agcgatgcaa
     3421 tatgcagcag gctgctgctc ttgtagttgt tcatgtagtt gtagttgttc atgttcttgt
     3481 acaagtgcat caacagcaga gcaataaatt aaaggtaaag cttggtctat ttagatcaag
     3541 cttttccaaa tttgataggt gagtgtatgg aaagtttaat gactattaag aatttaaaag
     3601 ttgatattaa taatagaaaa attctaaacc ttaataatag tactataaat atttatagta
     3661 aagataaaat agcactgata gggaaaaatg gcgcgggaaa aacgactttg attaattgct
     3721 tgttaaatga gataaaatat agtggcgtta ttgacagaaa gataaagtct aaagatatag
     3781 gaattgtttt tcaagagaat aagtatgggg atttaatcaa agtacatgaa ttaatttatt
     3841 taataacagc ttatagcaaa agaagtaaac aatttaaagc atttacgaaa gctttctcat
     3901 tagaacatct tataaataaa tttgttaaag atttatcctt gggggagcaa aagcgcttaa
     3961 ctgtaggatt agtattaaat gaacaaaaaa agttatacat atttgatgaa ttgacatctg
     4021 gattagacta tgaaaaaaga caaaatttat tacagaaaac taaagaaatc acccaaaata
     4081 aagcagtaat taaaattacg cattactttg atgaggttga aaattgggcg aataaattaa
     4141 ttattataga taaaggagaa ttattgtatt acggcagtat agatcaattt ttctcaaaat
     4201 tttatcacta tgcacttatt gaaatagaga atcaagaatt agattatcaa gttagagttg
     4261 ttattgaaaa aataaatgcc gatgtatatt ttaatactaa gattgttacc cataataaag
     4321 agcaaatgaa tgacgttatt aatatattaa atgaacgaaa aatagaattt aaaataaaca
     4381 tgcaaaatat atactcaaca tatttaata
//
LOCUS       DAHHRR010000160         3981 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.164, whole genome shotgun sequence.
ACCESSION   DAHHRR010000160 DAHHRR010000000
VERSION     DAHHRR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.164"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            48..1601
                     /locus_tag="NBI42_002447"
     CDS             48..1601
                     /locus_tag="NBI42_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCD1778833.1"
                     /translation="MSEEKKVVKHERQKNEKTKKQYKPFWIVMSFIILIVVLLLPTPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAVLTGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALIVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSIALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSAREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
     gene            1687..3060
                     /locus_tag="NBI42_002448"
     CDS             1687..3060
                     /locus_tag="NBI42_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCD1778834.1"
                     /translation="MAVAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIITEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVIAGDIMSYHLE
                     EYDIIHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYKVFTSFYKKWRPYLRHRD
                     IYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLSE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGLHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(3215..3499)
                     /locus_tag="NBI42_002449"
     CDS             complement(3215..3499)
                     /locus_tag="NBI42_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778835.1"
                     /translation="MTINIIVLIVSIIGFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(3496..>3981)
                     /locus_tag="NBI42_002450"
     CDS             complement(3496..>3981)
                     /locus_tag="NBI42_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCD1778836.1"
                     /translation="LRLFKPNKKYEWPFFIIYSFIFVLLLPNTFYMVTDLIHLNQFQF
                     NFYAGLNLSEWKYFTYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGF
                     GIYIGRFLRLHSVYFFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLN
                     K"
BASE COUNT         1348 a          584 c          728 g         1321 t
ORIGIN      
        1 aaaaaattgt tataattaaa ttaagattaa acaaaggggt gactgttatg tcagaagaaa
       61 aaaaggtagt taaacatgaa cgacaaaaga atgaaaagac aaaaaagcaa tacaagccat
      121 tttggattgt tatgagtttt ataatactta tagttgtatt attactcccg acaccttcaa
      181 gtctgccaat aatggctaag gcagtactag ctattttagc ttttgcagtt attatgtggg
      241 taacggaagc tgtatcatat ccggtgtcag caactttaat tattggctta atgatattac
      301 ttttaggatt tagcccggtt caaaatttag gagaaaagct gggtaacccg aaaagtggta
      361 gtgctgtttt aactggaagt gatcttctag gaacaaatca tgcattatca ttagcgttta
      421 gtggatttgc aacatcagct gtagctctcg ttgcagccgc attatttttg gctgctgcta
      481 tgcaagagac gaatttgcat aaaagactag ctcttatagt gctatcaatt gttggtaata
      541 aaactagaaa tatagttatt ggagcaatta ttgtttcaat tgtacttgca tttttcgttc
      601 cttctgcaac agctagagca ggggcagttg taccaatctt gctgggtatg attgcagcat
      661 ttaaagtttc taaagatagc aagttagcgt ctttattaat aattacttca gtacaagctg
      721 tgtcaatttg gaatattggt atcaaaacgg cggcagcaca aaatatcgta gcgattaatt
      781 ttataaacca tcaattagga tttgatgttt catggggcga gtggttctta tatgcagcgc
      841 cttggtccat agttatgtct atagctttat atttcatcat gattaaagtg atgcctccag
      901 aaattaatac aatagagggt ggcaaagatt taataaaaga agaattgcat aaacttggcc
      961 ccgttagcgc acgtgaatgg cgtttaattg ttatatcgat gttattatta ctgttttggt
     1021 caactgaaaa agtattacat ccgattgact ctgcatccat tactattatt gctttaggtg
     1081 ttatgttaat gccgaaaatt ggtgtcatga catggaaaca tgttgaaaat aaaataccat
     1141 ggggaacaat tatcgtgttt ggtgtaggta tttcgctagg taacgttctt ttgaaaacag
     1201 gtgcagctca atggttaagt gatcaaactt ttggtgtttt aggtttaaaa catttaccta
     1261 ttatcgcgac aattgcactt atcacgcttt ttaatatatt gattcatttg ggctttgcga
     1321 gtgcaacaag tttatcatca gcgttaatac ctgtttttat ttcgctaacc tctacgttac
     1381 acttaggaga ccagtctata ggatttgttt taattcaaca atttgttatt agttttggtt
     1441 tcttattacc tgttagtgca cctcaaaata tgttggctta tggcactggt acttttacgg
     1501 ttaaagattt cttgaaggca ggtataccat tgacaattgt agggtatatt ctagtgatag
     1561 tttttagtat gacttattgg aaatggttag gtttgcttta attaaaaata taaataagaa
     1621 tctaggttat tttaaagtga caaaaagctt aataaaataa aaagataatt gaagggtgtt
     1681 ttgtttatgg cagttgctgt gttattaaat cgaatgtttc gaatggaaca caatccatta
     1741 tttgaatata tttatcaaca aaaagaagac attgatgcat gttattttat cattacggaa
     1801 gaggacatgt cttcagcttc tgatttgaaa gcacagtttt atcgcggtac tttgcagcgc
     1861 ttttaccaat cgttgcacgc agaaaagctc acaccttatg tcatgtctta tgacgatatc
     1921 atttcatttt gtaaagaaaa caatatatct gaagtagtga ttgcgggtga tattatgagt
     1981 tatcatcttg aagaatatga tattatacat caacgttctt tattcaatga agcacgtatt
     2041 gccgttactt taatacgtgg gaatcattac tttaaagcga gtaaaacaat gaatcaacaa
     2101 ggggagccat acaaagtttt tactagtttc tataaaaaat ggcgacctta cttgaggcat
     2161 agagacatat atcactatga tttaaaatca ttcgaagact ttgtcattgc atcacctgat
     2221 gatttagtgt ttgatgacat agcgtttggt tcctcacaaa taattgaaca gaataaatgg
     2281 caacattttt tagatcaaga tatacagaat tacgaaagcg gaagagacta tttatctgaa
     2341 gtgttaacaa gtcagctaag tgttgcttta gcatatggat tattagatat tattgaaatt
     2401 tttaatgatt tattggcgcg ttatgatgaa gatgaggcca actatgaagc atttatacgt
     2461 gaactcattt ttagagaatt ttattatgtg ttaatgacac agtatccaga aacctcatac
     2521 caagctttca aacctaaata tcgacagata aaatggtcgc aaaatgaagc ggattttaat
     2581 gcatggtgcg aagggcaaac aggatttcca atcattgatg cagcaataat ggaattgaca
     2641 caaactggtt ttatgcataa tcgaatgaga atggttgtgt cgcaattttt aaccaaagat
     2701 ttatttatag attggacatg gggagaaaaa ttctttagaa agcaccttat tgactatgat
     2761 gcagcatcaa atattcatgg atggcaatgg tctgcttcta caggtacgga tgcagtgccg
     2821 tattttagaa tgtttaatcc aataagacag agtgaacgct ttgatgctaa agctttgtat
     2881 atcaaaacat atcttccgat ttttaatcaa attgatgcaa aatatttgca tgatacacaa
     2941 cgcaatgagt ccaacctttt tgaacagggg attgaattag gtcttcatta tccaagacaa
     3001 atggtagatc atcaagaaaa acgtacacaa gttttagcta catttaaagc gctagactaa
     3061 ttcggctcaa ttgatagatc aggcatgagt aaaatctgga gcagaatata aagttcttaa
     3121 gcaatataaa taagccaatt tctattattg atgtgataga aattggcttt cctcaaatat
     3181 ataagttgta tttagttttc ttattaattt gatgttatct tagtatgtcc gtaaataaag
     3241 tgaggtatag tacgacatac tctaaaaacg tagcgagata aatatatttc aatctaactt
     3301 ttatgttttg aggcacttgc catttaggat attgtcgttc gtaatacgac acttgttgta
     3361 taaatacacc tagtccaaat ggtagcatca tgagtaagat acttcttaaa taacttaaac
     3421 caatatcatg ccatatgtgt ccaataatca attgaaatcc aatgatagat actattaaaa
     3481 cgattatatt tattgtcact tgttcaaacg cactcctttt ccaaataata gaattgctgc
     3541 ttgcatcaca accataaaac atacaaacat agcagtttta agcgttagac tttctagaat
     3601 gtgatttaga acatgtaagg gctcattaaa gaaataaacg gaatgtaagc gtaagaaacg
     3661 accaatataa attccgaatc catttaaaaa cattagcacg acaacaatta atctattaag
     3721 ccaacggtga gaagtcaatg ttagtatttc aaaatagatt aaaatcatca cataaaccgc
     3781 taagaagaca ccaagcagta aataggtaaa gtatttccac tcacttaaat ttagtcctgc
     3841 gtaaaagttg aattgaaatt ggttcaaatg gattaaatca gttaccatat aaaatgtatt
     3901 tggtaaaagt aacacaaata taaaactata tatgataaag aatggccatt catacttttt
     3961 attcggtttg aataatcgta a
//
LOCUS       DAHHRR010000161         3943 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.165, whole genome shotgun sequence.
ACCESSION   DAHHRR010000161 DAHHRR010000000
VERSION     DAHHRR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.165"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..237
                     /locus_tag="NBI42_002451"
     CDS             <1..237
                     /locus_tag="NBI42_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778837.1"
                     /translation="DGYVNHNKNYEFKVLISATDNHQFEDSIGYDDKTFGKLFKEKDH
                     KNELKPTDIIKKEHLDKSEYEADPPLFFFSGPLE"
     gene            468..827
                     /locus_tag="NBI42_002452"
     CDS             468..827
                     /locus_tag="NBI42_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1778838.1"
                     /translation="MIVFIIIILILGGIFLKMKYDEMKYYDEQKERIKLFMKYNVKGF
                     KEIQFEEQKENQMDGYSISGYINNDKNLSFTAGIRTIDDFQFDTDISYTDELGRKIIK
                     NPKSVSEIKKEQNTSNK"
     gene            complement(951..3374)
                     /locus_tag="NBI42_002453"
     CDS             complement(951..3374)
                     /locus_tag="NBI42_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HCD1778839.1"
                     /translation="MTYKMKKWQKLSTITLLMAGVITLNGGEFRSIEKHQIAVADTNV
                     QTPDYEKLKKTWLDVNYGYDQYDENNQDMKKKFDAKEKEAKKLLDDMKTDTNRTYLWD
                     SAKDLDKKSADMTKTYRNIEKIAEAMRHKNTSLKTDENKLKITGAIKWLHHNVYGKDP
                     DKKVADLTTNRKEKDSSKKNNSLNWWDYEIGTPRALTNTLLLMDDMLTKDEMKSYSKP
                     ISTYSPSSDKILSSVGESEDAKGGNLVDISKVKLLESVIEEDVDMMKKSIDSFNKVFT
                     YVQDSATGKARNGFYKDGSYIDHQDVPYTGAYGVVLLDGISQMMPMIKESPFKHTQDK
                     ATLSNWIDEGFMPLIYKGEMMDLSRGRAISRENETSHTASATVMKSLLRLSDTMDEST
                     KTRYKQIVKTSVKSDSSYDSNDTLTSYSDIDKMKKLMDDKTISAKEQTQQLKIYNDMD
                     RVTYHNKDLDFAFGLSMTSKNVARYESINNENLKGWHTGAGMSYLYNSDVKHYRENFW
                     ATTDMTRLPGTTTLNDMQSTNTKNDKSFVGGTKLNNKYASIGMDFENQDKTLTAKKSY
                     FILNDKIVFIGTGIKSTDSSKKPVTSVENRKANGYKLYKDDIEITTSDVNAQETHSVL
                     LESSDTKKNIGYHFLDKPIITVKKETHTGKWKDINNSQSTTQKNDDYYEVTQTHNTSE
                     NKYAYVLYPGLSKSDFKSKNNNVSIVKQDEDFHVIKDNDGVFAGVNYSDSTKTFAING
                     ATVELKEKGMFILKKKDDKTYECSFYNPESTNSASDIESKISIAGYTITNKNVTNSND
                     AGVNFELTK"
BASE COUNT         1314 a          584 c          586 g         1459 t
ORIGIN      
        1 gatggatatg ttaatcacaa caaaaattat gaatttaaag ttctcatctc tgcaactgat
       61 aatcatcaat ttgaagatag cattggatac gatgataaga cctttggtaa attatttaaa
      121 gaaaaagacc ataaaaatga attaaaacct acagacatta taaaaaaaga acacttggac
      181 aaaagtgaat atgaagctga tccaccgttg ttcttctttt caggtccact tgagtaaatg
      241 atatgaagag tataggtatc atagacaata ttgtgatatg cattttaaag taaaccagat
      301 catgatttaa gattataaga aagaaaaatt ggctaatgtt aaaaccaaca gtcatatggt
      361 ttgaaatgtt tttacaatca ttgtaaaact cagtatgctt cattaattta aaatgttcgt
      421 caataaatcc aatgaaatgg agatgggttt tttgaaaaaa acctcttgtg attgtattta
      481 taattataat attgattcta ggcggaatct ttttgaagat gaaatatgac gaaatgaaat
      541 attatgatga gcaaaaagaa agaatcaaat tatttatgaa atataatgtt aaagggttta
      601 aagaaataca attcgaggaa caaaaagaaa accaaatgga tggctattct attagtggtt
      661 atataaataa tgataaaaat ttatcattta cagctggcat aagaactatt gatgattttc
      721 aatttgatac tgatatttct tatacagatg aattgggtag aaaaattatt aaaaatccta
      781 agtcagtttc tgaaataaag aaagagcaaa atacgtccaa taaataatag ttcatattgt
      841 gatggatcaa aaatataagt cgttagatga atttaaatat gatataaaat cttgcaatgc
      901 ttataaaatc tcgacactat aaaaatagta tagtgccgag tttttattaa ttatttagtt
      961 aattcaaagt ttacaccagc atcatttgag tttgtgacat ttttattagt tatagtgtaa
     1021 cctgcaattg aaattttaga ttcaatatct gaagcggaat ttgtagattc gggattatag
     1081 aagctacatt catatgtttt atcatctttc tttttaagta taaacatacc tttttctttt
     1141 aattcaacag ttgctccgtt tatagcaaaa gttttagtac tatcactata attaacccca
     1201 gcaaatacgc catcattatc ttttatcaca tgaaaatctt catcttgttt aacaatactt
     1261 acattattat tcttcgattt aaaatcactt tttgataagc caggatacaa aacatacgca
     1321 tatttatttt cagacgtatt atgtgtttga gttacttcgt aataatcatc atttttttga
     1381 gtagtgcttt gactgttgtt aatatctttc catttgcccg tatgtgtttc tttttttaca
     1441 gttattattg gcttgtctaa gaaatggtaa ccaatgttct ttttagtatc gctggactct
     1501 aataagactg aatgggtttc ctgagcatta acatctgaag tggtaatttc aatatcatct
     1561 ttatataatt tatacccatt tgctttgcga ttttcaacac ttgtaactgg tttctttgat
     1621 gaatcagtac ttttaatgcc agttcctatg aagacaattt tatcgtttaa tatgaaatat
     1681 gattttttgg cagttaaagt tttgtcctga ttttcaaaat ccataccgat gcttgcgtat
     1741 ttattattta attttgtccc gccaacaaaa gatttatcat tcttagtatt agtagattgc
     1801 atatcattta aagtagtagt acctggaaga cgagtcatat cagttgtcgc ccaaaagttt
     1861 tcacggtagt gtttcacatc gctattgtat aaataagaca ttccagcacc agtatgccag
     1921 ccttttaggt tttcattatt gatactttcg taacgtgcga cgtttttcga cgtcatactt
     1981 aatccaaatg caaagtccag gtctttattg tgataggtga cacgatccat gtcattatat
     2041 attttaagtt gttgtgtttg ttctttagca gagatagttt tatcatccat taactttttc
     2101 attttatcta tgtctgaata agaagttaat gtatcatttg agtcataact tgaatcagat
     2161 ttaacagaag ttttgacgat ttgcttatat ctagtttttg ttgactcatc catggtatca
     2221 ctcaatctca acaatgattt cattacagtc gctgacgctg tatgactcgt ttcattttca
     2281 cgactgatag ctctaccacg tgataaatcc atcatttcac ctttatagat taatggcata
     2341 aacccttcgt caatccaatt gcttaatgta gctttatctt gtgtatgttt aaaaggagat
     2401 tcttttatca tcggcatcat ttgagaaatt ccatctaaga ggacaacgcc ataagcacca
     2461 gtgtagggga catcttgatg atcaatgtaa gagccatctt tatagaatcc attgcgagct
     2521 ttaccagtgg cagaatcttg aacataagtg aacactttat taaaagaatc tatagacttt
     2581 ttcatcatat ctacatcttc ttcaataaca ctttctaaaa gttttacttt agaaatgtct
     2641 actaaatttc cacctttagc atcttctgat tcaccaacag aagataaaat tttgtcactg
     2701 gatggtgaat atgtactaat aggttttgaa taacttttca tttcatcttt agtgagcata
     2761 tcgtccatta gaagtagtgt gtttgttaat gctcttggcg taccaatttc ataatcccac
     2821 caattcaatg agttattctt tttactagaa tctttttctt tacgattggt ggttaaatct
     2881 gcgactttct tatctggatc cttaccatat acgttatgat gtaaccactt aattgcacca
     2941 gttattttca acttgttttc atctgttttt aatgaagtat ttttatgtcg cattgcttct
     3001 gcgattttct cgatattgcg ataggttttt gtcatgtctg cggacttttt atccaaatct
     3061 tttgcactat cccacaaata tgttctattc gtatcagttt tcatgtcatc aagtaacttc
     3121 ttggcttctt tttctttagc atcaaacttc ttcttcatat cttgattatt ctcatcatac
     3181 tgatcataac cgtagttaac gtcgagccac gtcttcttca atttttcata atctggcgtt
     3241 tgaacattcg tatcagccac agcgatttga tgtttttcaa tacttctgaa ttcaccacca
     3301 ttcaaagtaa ttacccctgc cattaataac gtaatggtgg ataatttttg ccatttcttc
     3361 attttatatg tcattgacct gtctcctttt ttgcgtgtgt gcaattgata ttatgattaa
     3421 ttaatgattc aactttccaa aggattggga aattcgttga tgcatggaaa ccatcagtca
     3481 ccatgaaatc tagtttttat ctaaaaatgt aaaaaaatta aaattggaaa attaaatatt
     3541 aataatctta tcgattatag caacttaaat aattaattta tacaatattt aaagtaatta
     3601 aaattgatgt aaagtaaaat gttagaaaag agacaaattt tgatttaagt aataaaatga
     3661 taagtgaact taaaaagata ttaatttaga ataagattgc tattttatac ataataagaa
     3721 aagcaaaaca gcgcgacttt atgtatttaa tactatgtca agcaagtaat ttttaaaagg
     3781 tattttgaaa caacgaatca tgagaggcgt aaacggaaca tagaaaaaag atagcatgtc
     3841 aaagtaaatt ggtttttgaa atctaaaatc tataagaaat aaatactact ctaggggatt
     3901 aaaatgaaaa aaagatatgt atttatggtt gttgttgcaa tat
//
LOCUS       DAHHRR010000162         3933 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.166, whole genome shotgun sequence.
ACCESSION   DAHHRR010000162 DAHHRR010000000
VERSION     DAHHRR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.166"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(353..1054)
                     /gene="isaA"
                     /locus_tag="NBI42_002454"
     CDS             complement(353..1054)
                     /gene="isaA"
                     /locus_tag="NBI42_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HCD1778840.1"
                     /translation="MKKTIMASSLAVALGVTGYAASTGHEAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLSAWGF"
     gene            complement(1662..2717)
                     /locus_tag="NBI42_002455"
     CDS             complement(1662..2717)
                     /locus_tag="NBI42_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCD1778841.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            complement(2844..2972)
                     /locus_tag="NBI42_002456"
     CDS             complement(2844..2972)
                     /locus_tag="NBI42_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778842.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSKNSEFCDILFFIVLSYR"
     gene            3127..3696
                     /locus_tag="NBI42_002457"
     CDS             3127..3696
                     /locus_tag="NBI42_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1778843.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
BASE COUNT         1355 a          703 c          590 g         1285 t
ORIGIN      
        1 tattcgaatt tactttttca aagttgaata aaataacaaa ttaacgcgaa ctgttattaa
       61 aatatataca cggtgatatc tttccaactc actttcaaat ttaaccatat gaaatgacaa
      121 atactcactc catatttaaa aagaatcatg gtgtattgtt tatttcgcct tattctaatt
      181 taatagtgac attaattctt ctatcatgac attaaaatcg tcaggaataa taaaataaca
      241 tcctaacctc tagaatcaga cattaatttc taataaaaaa actctcccca atttctatgg
      301 gaagagctat atatttaatg tctaaacatt acttttatta attatgaagg aattagaatc
      361 cccaagcgct taaaccttgt gctttgtatg ctttaacagc tgcgttgatt tgttggtcaa
      421 cagtgtttgt tggaccccaa cctggcatag tttggaataa acctgaagca cctgatgggt
      481 tgtaagcatt tacttgacca tttgattcac gagcgatgat tgcagcccat gtagaagctg
      541 aaacaccagt acgttgagcc atgatttgag ctgctgatga accagtagca cctgcagtat
      601 taccattgct taatctcact gaacttgaag tagttgaagt gctgtagtta tggtaagttg
      661 gagctgaaac agcttcaacg tttgagttac ttgattgtgc attgtagctt actgattgta
      721 catttgaacc ttggttgtat gaagtagtgt agtctgcacc tgcaacgttt gagaaaccag
      781 cagtttgacc attagctgct tcatagctcc atgaccatgt agtaccattt gaagtgaagt
      841 tatattggaa accatctttt acaaagtgga tgtcatatgc accatctttg attggagctg
      901 catttaattg atcttggtga ttgtgtgcta agtcaactaa gtgtgcttga tcaacgttta
      961 cttcagcagc gtgtgcttca tgtcctgtgc ttgctgcgta acctgttaca cctaatgcca
     1021 ctgctaatga tgatgccata attgtctttt tcatagtaaa aaatcctcca gtaataattg
     1081 taagtttatg tttttagtaa ttatattttg aatttgaatg tcgtagtgca agtttaaatt
     1141 gtcttttatt tctttcaacg gtactcacta tatcacaaaa aaccagccag taaattacac
     1201 tttctttaca aaacattaca atatcgagtg tgatttgtaa tgttgaaata tggttgtttt
     1261 atactgtaat gagaaatata actctttttg aatcttattt attcttgaaa tagcaagaaa
     1321 aacataagtt ttttactata tttagctcaa ctattacagc tttcgtaata ttacagattg
     1381 tatttttgtt acatagctgt aatatatctg acatgtaact tctcttattt tcgtacatta
     1441 aacgcaatta aaaagcaatc aacacatacg gttcttcaaa tgtgttgatt gctattattg
     1501 ttatatattc aaagatacta tgcacacatc ttttgtgaat tgtcttatct tttataaaca
     1561 caaataaact acagctcaat tatattgtta aactttattc tctcaattca caataacatt
     1621 aaataatttt tggtctcata ttttcaaaaa acatactgtc cttatcccat gaatttaaaa
     1681 atatcattag tatataaacg aaacacttta cgataaatga tatctgcaag ccaagctgtt
     1741 acaaatggta caacaaagaa cgctactaca attagtaaga cactcaacca agcagaatca
     1801 acctccataa atttaaatgc attaatcggt cctaccatcc ctataaaacc aaatccagct
     1861 gactctttcg ttccatgaat acctactaat gctgatacca aacctgatac aatggctgtc
     1921 gtcaatattg gtaacataag aattggatat ttcaccatat taggtatcat cattttaacg
     1981 cctccaaaga agacggataa cggcacccct aaacgattca ctttacttgt accaattatc
     2041 aatactgctt cagttgcgga aataccaatt gacgcagacc cagctgctaa acctgtaata
     2101 cctatcgcaa aggcaatggc cacagttgat agtggagaaa taataataag actaaatacc
     2161 attgaaatca aaatactcat gacaatcggt tgtaattctg taaaaccatt aaccatatta
     2221 ccgatggctg ttgtaatcat tttcgtatac ggcaatatta aaacaccaat tgcacctgaa
     2281 ataccgccaa caactgttgg gaatacaatc aatgccatac tacctacgcg atgttgaata
     2341 agtaaaatga acaacactgc aatcgctgct gtaatcattg tattaattaa atcaccaata
     2401 cccgtaatca tccaagcacc atttttaaac tgcgctgcac cgcttcctac atatgctgca
     2461 cttgccacaa cagcaattgc taatggcgat aggtcaaatt tcatggcaac caatgcacca
     2521 atcaaagcag gtactgtaaa ttgaattgca acgacaacgc ctaataacgt tttaaaaatc
     2581 ggatgataat ccataaagta tttaaaaatt tctccaagta tcgcattagg aactaaaccc
     2641 gcaacaatac ctatggcgac acctgataaa actctaaata taaaatcttt gggtgtaatt
     2701 gttttaattg atgtcataat atcatccttc catttatgta tatacatctt tatgcaaata
     2761 ataaagagcc ttaagttata agctgccact agcttaaatt ctaagatgtg catgccgatg
     2821 ttgttatatt taggctagca gtatcatcta taactcaaga ctatgaaaaa tagtatatca
     2881 caaaattctg aatttttaga taaagaaatt ggcaattttt caaacatatt gttacaatac
     2941 acttttattt tatcttcatt tttaaaatcc attaatataa tagaagaaag acattcaaat
     3001 gcttaccaaa aaggtacatt atttgttagg agcgtatcag cacttacata tcatcaacac
     3061 cattgacaac ataatagaag atactgataa taagtgttaa aacaacagat gttaggtagt
     3121 gaacaaatga tggaaagtaa atccatagat ccaagaatcg ttagaaccaa acaattgctt
     3181 gtcgatgctt ttcttaaaat ttctagagaa aagaaattat ctcaaattac agttaaagat
     3241 atcactgata ttgctacgct aaatcgtgca acattttacg ctcatttcac tgataaagaa
     3301 gacctcctag actacacatt gtctgtaacc attttaaaag atttgaatga taacttgagc
     3361 atttctaatg tcattaatga aaaggtactg cgtaatattt tcatttcaat tgcgagttat
     3421 atcaaagatg ctgcaaagtc ttgcgaatta aatagtgaag cattttgcaa caaagcacat
     3481 caacgtatta ataatgaatt agaagatatt tttgcgatta tgttagaaaa cagctatcca
     3541 gagcatcaac gagatatcat tgtaaatagt gcgagttttt tagcagctgg tatatcaggc
     3601 ttagcattac attggtttaa cacgagtcaa gagacagccg atgtgtttat cgatcgcaac
     3661 cttccatttt taattcatca tatagcacat ttttaataaa acttggtatt tagtcatgca
     3721 tcttgaaatc actatgtgac ttaggttcat acttgtacac acaataaaat ttaacgtatt
     3781 acgattgatt agccgtgtct aggacataaa tcaacgtcct atactctaca atgccatatt
     3841 ggcagtcgtt aactgaatga aaataagagc ttgggacatt aagttcttag gcaatgtaaa
     3901 aaagctgatt tctattaatt atttgataga aat
//
LOCUS       DAHHRR010000163         3837 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.167, whole genome shotgun sequence.
ACCESSION   DAHHRR010000163 DAHHRR010000000
VERSION     DAHHRR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.167"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            526..1269
                     /locus_tag="NBI42_002458"
     CDS             526..1269
                     /locus_tag="NBI42_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCD1778844.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            1366..1794
                     /locus_tag="NBI42_002459"
     CDS             1366..1794
                     /locus_tag="NBI42_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCD1778845.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(1840..3837)
                     /gene="mecA"
                     /locus_tag="NBI42_002460"
     CDS             complement(1840..3837)
                     /gene="mecA"
                     /locus_tag="NBI42_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCD1778846.1"
                     /translation="MKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFKQVYK
                     DSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKIKTNY
                     GNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNNVELA
                     NTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTVKKMD
                     EYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDDAVIG
                     KKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIY
                     NNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPLLNKF
                     QITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVVNGNI
                     DLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKNLDNE
                     ILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISKENIN
                     LLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKDNPNM
                     MMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
BASE COUNT         1278 a          576 c          621 g         1362 t
ORIGIN      
        1 aaagcagttc tcagtaaaat tacagataag aggtacgtta aaagcagttc taagtaaaat
       61 tgcagataag aggtttgtta aaagcagttc taagtaaaat tgcagataag aggtacgtta
      121 aaagcaattc catgcaaaat tgctgataag gggtaagtta aaagcagttc tcagtaaaat
      181 tgcagataag aggtacgtta aaagcagttc taggcaaaat tgcagataag aggtgcgtta
      241 aaagcagttc tcagtaaaat tgctgataag gggtaagtta aaagcaatcc taagtaaaat
      301 tgcagataag gggtacagaa aaactagact tgattacaaa atggagcttg ggacataaat
      361 gattttttaa aaatgagatg agacgtagat taactccata atcaatacga atctatcgac
      421 ttctttattt atgatattca tctcttttta atggaaataa aagtgcgatt aatgtgataa
      481 tacagttacg ttaattaaaa aaataaaaat gcaaggagag gtaatatgct aactgtatat
      541 ggacatagag gattacctag taaagctccg gaaaatacaa ttgcatcatt taaagctgct
      601 tcagaagtag aaggtataaa ctggttggag ttagatgttg caattacaaa agatgaacaa
      661 ctgattatca ttcatgatga ttatttagaa cggactacaa atatgtccgg ggaaataact
      721 gaattgaatt atgatgaaat taaagatgct tctgcaggat cttggtttgg tgaaaaattc
      781 aaagatgaac atttgccaac tttcgatgat gtagtaaaaa tagcaaatga atataatatg
      841 aatttaaatg tagaattaaa aggtattact ggaccgaatg gactagcact ttctaaaagt
      901 atggttaagc aagtggaaga acaattaaca aacttaaatc agaatcaaga agtgctcatt
      961 tcaagcttta atgttgtgct tgttaaactt gcagaagaaa tcatgccaca atataacaga
     1021 gcagttatat tccatacaac ttcgtttcgt gaagactgga gaacactttt agattactgt
     1081 aatgctaaaa tagtaaacac tgaagatgcc aaacttacta aagcaaaagt aaaaatggta
     1141 aaagaagcgg gttatgaatt gaacgtatgg actgtaaaca aaccagcacg tgcaaaccaa
     1201 cttgctaatt ggggagttga tggtatcttt acagacaatg cagataaaat ggtgcatttg
     1261 tctcaataga aagttagagg tgagtcttac gtttcagtga cggtagactt atctttaaca
     1321 tgttacatac taaaaaatta atttgaataa gaaagagaga catatatgaa atacgatgat
     1381 tttatagtag gagaaacatt caaaacaaaa agccttcata ttacagaaga agaaattatc
     1441 caatttgcaa caacttttga tcctcaatat atgcatatag ataaagaaaa agcagaacaa
     1501 agtagattta aaggtatcat tgcatctggc atgcatacac tttcaatatc atttaaatta
     1561 tgggtagaag aaggtaaata cggagaagaa gttgtagcag gaacacaaat gaataacgtt
     1621 aaatttatta aacctgtata cccaggtaat acattgtacg ttatcgctga aattacaaat
     1681 aagaaatcca taaaaaaaga aaatggactc gttacagtgt cactttcaac atacaatgaa
     1741 aatgaagaaa ttgtatttaa gggagaagta acagcactta ttaataattc ataataaaac
     1801 agtgaagcaa ccatcgttac ggattgcttc actgttttgt tattcatcta tatcgtattt
     1861 tttattaccg ttctcatata gctcatcata cactttacct gagattttgg cattgtagct
     1921 agccattcct ttatcttgta catctttaac attaatagcc atcatcatgt ttggattatc
     1981 tttatcatat gatataaacc acccaatttg tctgccagtt tctccttgtt tcattttgag
     2041 ttctgcagta ccggatttgc caattaagtt tgcataagat ctataaatat cttctttatg
     2101 tgttttattt acgacttgtt gcataccatc agttaataga ttgatatttt ctttggaaat
     2161 aatatttttc ttccaaactt tgtttttcgt gtcttttaat aagtgaggtg cgttaatatt
     2221 gccattattt tctaatgcgc tatagattga aaggatctgt actgggttaa tcagtatttc
     2281 accttgtccg taacctgaat cagctaataa tatttcatta tctaaatttt tgtttgaaat
     2341 ttgagcatta taaaatggat aatcacttgg tatatcttca ccaacaccta gttttttcat
     2401 gcctttttca aatttcttac tgcctaattc gagtgctact ctagcaaaga aaatgttatc
     2461 tgatgattct attgcttgtt ttaagtcgat attaccattt accacttcat atcttgtaac
     2521 gttgtaacca ccccaagatt tatctttttg ccaaccttta ccatcgattt tataacttgt
     2581 tttatcgtct aatgttttgt tatttaaccc aatcattgct gttaatattt tttgagttga
     2641 acctggtgaa gttgtaatct ggaacttgtt gagcagaggt tcttttttat cttcggttaa
     2701 tttattatat tcttcgttac tcatgccata cataaatgga tagacgtcat atgaaggtgt
     2761 gcttacaagt gctaataatt cacctgtttg agggtggata gcagtacctg agccataatc
     2821 atttttcatg ttgttataaa tactcttttg aactttagca tcaatagtta gttgaatatc
     2881 tttgccatct tttttctttt tctctattaa tgtatgtgcg attgtattgc tattatcgtc
     2941 aacgattgtg acacgatagc catcttcatg ttggagcttt ttatcgtaaa gtttttcgag
     3001 tcccttttta ccaataactg catcatcttt atagccttta tattcttttt gttttaattc
     3061 ttcagagtta atgggaccaa cataacctaa tagatgtgaa gtcgcttttc ctagaggata
     3121 gttacgactt tctgtttcat tagttgtaag atgaaatttt tttgcgaaat cacttaaata
     3181 ttcatccatt tttttaacgg ttttaagtgg aacgaaggta tcatcttgta cccaattttg
     3241 atccatttgt tgtttgatat agtcttcaga aatacttagt tctttagcga ttgctttata
     3301 atctttttta gatacattct ttggaacgat gcctatctca tatgctgttc ctgtattggc
     3361 caattccaca ttgtttcggt ctaaaatttt accacgttct gattttaaat tttcaatatg
     3421 tatgctttgg tctttctgca ttcctggaat aatgacgcta tgatcccaat ctaacttcca
     3481 cataccatct tctttaacaa aattaaattg aacgttgcga tcaatgttac cgtagtttgt
     3541 tttaatttta tattgagcat ctactcgttt tttattttta gatacttttt ttattttacg
     3601 atcctgaatg tttatatctt taacgcctaa actattatat atttttatcg gacgttcagt
     3661 catttctact tcaccattat cgcttttaga aatataactg ctatctttat aaacttgttt
     3721 gaaattttta tcttcaattg catcaatagt attattaatt tctttatctt ttgaagcata
     3781 aaaatatata ccaaacccga caactacaac tattaaaata agtggaacaa tttttat
//
LOCUS       DAHHRR010000164         3791 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.168, whole genome shotgun sequence.
ACCESSION   DAHHRR010000164 DAHHRR010000000
VERSION     DAHHRR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.168"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(301..1947)
                     /locus_tag="NBI42_002461"
     CDS             complement(301..1947)
                     /locus_tag="NBI42_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCD1778847.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
     gene            2151..2513
                     /gene="mscL"
                     /locus_tag="NBI42_002462"
     CDS             2151..2513
                     /gene="mscL"
                     /locus_tag="NBI42_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCD1778848.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            complement(2584..3732)
                     /locus_tag="NBI42_002463"
     CDS             complement(2584..3732)
                     /locus_tag="NBI42_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001806960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCD1778849.1"
                     /translation="MRLNKNESELKLCRDLITEFETLSKYNNITNFEVDYKKYVQDVN
                     QHQELSKEIEDKLIQLSQRKLIEQNNLNHYENQLETYNNDLELNEQSIEMEMSRLNLT
                     DDNDINEIIAWRGEQEKLEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLLDEY
                     ELKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGK
                     NNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLH
                     SNKSRHISSLSGGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLET
                     ALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
BASE COUNT         1263 a          673 c          544 g         1311 t
ORIGIN      
        1 actttaatat ttcttttcaa tgtcattctt ttgatataca cataccgaaa tatgtatagt
       61 taatttttgt ttataccgtc gtacttcact ttagctttgt caaacccctt tctattaagt
      121 tgtcagaaat aaacctatct taaaatataa aaaaatcgag aattagtagt ttaataacga
      181 aattctcgtt cttatccttt tgaatatact caattttcca caaaaacaaa caagtagtat
      241 atctgttcta gctactagaa tgacatacta cttgttatta aaatacttaa ctaaatttta
      301 ttaattatct tttttctcta tatttctacg tgactgacgc ttctcaagaa tgtcagattc
      361 ataatcttct tgttgactct tgatatattc ttgtaagcgg tgtttattcg gagtcaatgt
      421 taaacctagg aatttacgtt cctggttcgc atccttgtag aaacttacca tcatgagtat
      481 gacgacaaag gagaatggga atgcacttat aattgcagca ctttgaatcg catttaaagc
      541 ttcagcgccg ttaccgccac cagctaataa aagtacaaat gctattaagg cctgtgaaat
      601 tccccaaaca acttttacca tactagatgg atttaatgaa ccaaatgttg tttgcattcc
      661 taatacaaat gttgctgagt cagcagatgt aataaagaat gatgcaatta ataataatgc
      721 aatcaacgat aaaacaatgc caaatggcac atgattaaac actccaaata gctgtgtttc
      781 aggagtcata tcaaaaattt ctttgtgttt cttacctgtt tcgatgccta atacaccaaa
      841 gacactaaac catacaaaac taacaattgc tggaactagc aagacaccag aaatgaactc
      901 tctaattgat cgtccttttg aaactcgtgc aataaacact ccaacgaatg ggctccaact
      961 taaccaccaa ccccagtaat aaagtgtcca tgaagacatc cattcacgtt tttgcggatt
     1021 taaagcagct gtatcaaagc tattaaacaa gaatgtgttt agtaaactac ccgtagagct
     1081 agttaacata tttaaaataa gaacagttgg tccaacaatt aaagcagcta ccattaaaat
     1141 agtacctaaa ccaatgttca agttacttaa gtattgaata cctttactta atccagacca
     1201 tgcacttgct ataaataaga tagtaacaac aatgatgata atcgcttgta caaacgtatt
     1261 gtttggaaca ttgaacaagt aatgtaaacc accattaatt tgtagagcac ccatacctaa
     1321 cgaaacggct accccaacga ttgtcgcaaa tacagataaa acgtcaataa aaatcccaat
     1381 aggtccttcc actttatcac ctaaaagagg acgtaaagtt ctagataata aacctggttc
     1441 acctttacgg aattgcgaat atgccaacgc taacgcaaca acaccataaa cagcccaagc
     1501 atggaatccc caatggaaaa atgttgaacg tagagcttca gtataagctt cagtagtttt
     1561 gggatctgct gtaggtggcg tagcaaagtg cgccatcggt tcagctgcac cataaaacac
     1621 caaacctatc cccataccag cactaaacaa catagcaaac catgaaattg tattaaactc
     1681 aggtttgtca tttggtttac ctagtttaag ttttccaata ggactaaaaa taaggaatat
     1741 acagaagaac acgataatcg tagtaagaat aagataatac caacctaact tttctgtaat
     1801 ccacatttta atattattgg taacatagtt gaattgttca ggtaaaaatg caccaagtaa
     1861 tacgactata gcaacaacaa ttgcactata gatgaagact ggtgaatact tctttccatt
     1921 tggattctct ggtgaagaag aattcataat taattactcc cttcaattct atatttaatt
     1981 ttatgtagta gaataaaaat attatctaaa cattttattc aataactcac gcacctttat
     2041 aggataacaa aggtctttgc gataatcaaa ttttcttgaa taatctattt tctaattttt
     2101 ttatttgttg tataattgag gttacattta aaaaagagag gttgagtgta atgttaaaag
     2161 aattcaaaga gttcgcctta aaaggtaacg tcttagattt agcaattgct gttgtgatgg
     2221 gtgcagcttt caacaagatt atatcttcat tagtagaaaa tatcatcatg ccattaattg
     2281 gtaaaatttt cggatcagtt gattttgcta aagaatggtc attctggggt attaaatacg
     2341 gtttatttat ccaatctgtt atcgacttta ttatcatcgc gtttgcttta ttcatctttg
     2401 ttaagattgc aaatacatta atgaagaaag aagaagccga agaagaagca gttgtggaag
     2461 aaaatgttgt gttattaact gaaatcagag atttattacg tgagaaaaaa taatttttaa
     2521 ataaaaagag atgggctaag gttgttctat acattccaaa gtaccatctc tattttttat
     2581 gttttaattt cttttgaatc ttgttgaact ctgatattga tctgatttca cctctaaaac
     2641 taaaggtatt ctatttttca attcgctcac atgtgaaata atcccaacca ttctaccagt
     2701 tgatttaaga tttaacaaag tgtctaacgc tgtttcaagc gtttcttgat ctaatgtacc
     2761 gaatccttca tcaataaata ttgattctag tgaaatacct cctgattgct gctgtacaat
     2821 ttcgcttaac cctaaagcta atgcaagcga cgattggaaa gtttctccac ctgataacga
     2881 gctaatatgt ctagacttat ttgaatgcaa atcaaataca tcaatttcta ggccactaag
     2941 accatgagaa accgcttctc gcctaattag ttggtatcga ttatctgaca ttgttgctaa
     3001 tctcagattt gcttgagcaa taatttgatc taaatagtaa attaagacaa agttttccaa
     3061 tgtaagattt ttgttattct taccactgac aatctcagcc aattgaaata tttcttgttg
     3121 gtcctttaat tcttgattta agtaattaat atgcgaaact atagattgtg ttttattaat
     3181 attattttga cattgataat gaacagcaga gtattcatct atcagtgtat tcatctttcc
     3241 tttttttagc tcatattcat ctaataattt atcagagtcc aataactctt tatccttggt
     3301 taatgattct aacctagcta tttccatttc aaattcatga taacgttttt tataagtatc
     3361 ccttttctgc tctaatttct cttgctcgcc cctccaggct attatttcat ttatatcatt
     3421 gtcgtcagtt aaattcagcc tcgacatttc catttcaata gattgttcat tcaattctaa
     3481 gtcattattg taagtttcta gttgattttc atagtgattc agattatttt gctcaattaa
     3541 ctttctttga gacaattgta ttaacttatc ttcaatctcc tttgagagtt cttgatgctg
     3601 attcacatct tgaacatact tcttataatc cacctcaaaa ttagtgatat tattatattt
     3661 cgagagtgtt tcaaattcag ttatgagatc tcgacataat ttcaactcag attcattttt
     3721 atttagacgc aattgtttat tatgaatttg caacgttaga ttatcttttt cttctttcat
     3781 ttgttctata a
//
LOCUS       DAHHRR010000165         3762 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.169, whole genome shotgun sequence.
ACCESSION   DAHHRR010000165 DAHHRR010000000
VERSION     DAHHRR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.169"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..674)
                     /locus_tag="NBI42_002464"
     CDS             complement(<1..674)
                     /locus_tag="NBI42_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1778850.1"
                     /translation="MKISKLKYLLLNFIFILILSGCSKEGIYSDKGQVFRKVIPQDMS
                     TLDTTLITDAVSGDISNQVFEGLFTLDKNDKAKLAIAKELPKKSNGGKTLTFKLRKDA
                     KWSNGDPVTAYDFVYAWRKLVDPKTGSEYAYIMSDIQNADSINSGKLPVEKLGIKALS
                     KYELEIKLKRPIPYINELLALSNFYPQNEKVAKKYGEKYGLKPQNAVYNGPFKVDTWR
                     AEDKIL"
     gene            complement(703..1629)
                     /locus_tag="NBI42_002465"
     CDS             complement(703..1629)
                     /locus_tag="NBI42_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1778851.1"
                     /translation="MNNNVLVEIKNLTKIFNKNKNNEIKAIDDVSININKGEALGIVG
                     ESGCGKSTFGKSIIRLLEVPEETIFFEGKDINKITSRRDSLNLNKRIQMIFQDPYASL
                     NPRLKVMDIVGEGIDIHKLAENKAERNEKIYKLLELVGLNKEHANRYPHEFSGGQRQR
                     IGIARALSVEPDLIIADEPISALDVSIQAQIINIIKKLQKERNTTLLFIAHDLSMVKY
                     VSDRIAVMHKGRIVEIASSERLYQNPLHNYTKSLISAMPQPDPQYERERVREIYKDSF
                     DNNSEYKLREIEKDHFVFTSINKAELLRQPKL"
     gene            complement(1619..2698)
                     /locus_tag="NBI42_002466"
     CDS             complement(1619..2698)
                     /locus_tag="NBI42_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010886477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1778852.1"
                     /translation="MKKVLEVKNLEVSFEVEDGIVNAVRGIDYCIHEGEILAIVGESG
                     SGKSVATKAITKLFQGENGKITNGYVKFFWEDLVEKNEKELAKIRGKDIAMIFQDPMT
                     SLNPTMKIGKQIMEPLMIHKKMNRKDAKGKAVEILDLVGIKNAELRFKDYPHQFSGGQ
                     RQRIVIGMALACEPKLLIADEPTTALDVTMQSQIIDLMKILQKKTNTAIIFITHDLGV
                     VANIADKVSVMYCGEIIETGKVEELFYYPKHPYTWGLLASMPTLESNSKSKLIAIPGT
                     PPNLLKPPIGDAFARRSAYALNVDFKYEAPWFQLSPTHFVKSWLLDSRAPKVIPPETV
                     KCKFKELSYNFERPLKVEGVSFNEQ"
     gene            complement(2714..3733)
                     /locus_tag="NBI42_002467"
     CDS             complement(2714..3733)
                     /locus_tag="NBI42_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1778853.1"
                     /translation="MVIDENKKQLTSTDFEFKKDKINQEFLNVKNKSFWQDGWSQLRK
                     NKLAVIGMIGLIMIILFALFAPAVSQHNSTEQNVTDRNLPSKIPLLEKVDYLPFNGKD
                     VNGKDTYKEVGSKDYYLFGTDQLGRDLWSRTWEGARISLFIGVVAAILDIFIGVLFGA
                     ISGFYGGKLDIIMQRIIEIIASIPNLIIVILFVLIFEPSIWTIILAMSVTGWISMSRV
                     VRGEFLKTKNLEFVQASKALGASNLRLIFKHILPNTLGAIIVTSMFTVPSAIFFEAFL
                     SFIGIGVPAPQTSLGSLVNDGKSMLLIYPHELFIPATILSLLILFFYLFSDGLRDAFD
                     PKMRK"
BASE COUNT         1157 a          702 c          467 g         1436 t
ORIGIN      
        1 aaaagtatct tatcttctgc tctccaagta tcaaccttaa acggcccatt ataaactgca
       61 ttttgaggtt tcaaaccgta cttttctcca tactttttcg caactttttc gttttgtgga
      121 taaaaattac ttaaagctaa caattcattt atgtatggta taggtctttt caactttatt
      181 tcaagttcat atttacttaa tgctttaata cctaactttt caactggtaa tttaccagaa
      241 tttatactat ccgcattttg aatgtcagac attatgtaag catattcaga tcctgttttt
      301 ggatcaacta attttctcca cgcatataca aaatcatacg ctgttactgg atctccgtta
      361 gaccatttag cgtcttttct taatttaaaa gttaaagttt taccaccatt acttttttta
      421 ggtagttctt tggctatcgc taatttcgct ttatcatttt tatctagtgt aaaaagtcct
      481 tcgaatactt gatttgatat atcacctgaa actgcatctg ttattaaagt tgtatctaaa
      541 gtactcatat cttgtggtat tactttcctg aaaacttgtc ctttatcaga atatatacct
      601 tctttactac aaccagagag tatcaatata aaaataaaat ttaataataa gtactttaat
      661 tttgaaattt tcataattta gttttccccc tttctaaaag cattaaagtt ttggttgtct
      721 caatagttct gccttattta tacttgtgaa cacaaaatga tctttttcta tttcccttag
      781 tttgtactca gaattattat caaacgaatc cttgtaaatt tccctaactc tttctctttc
      841 atattgtggg tctggttgag gcattgctga aattagtgat tttgtataat tatgtaatgg
      901 attttgatat agtctttcag aactagcaat ctcaactatt cttcctttgt gcatgactgc
      961 aattctatct gaaacatatt taaccattga taaatcatgt gcaataaata aaagtgtagt
     1021 attcctttct ttttgtaatt ttttaatgat atttattatt tgtgcttgta tcgatacatc
     1081 cagagctgaa ataggttcat ctgcgataat caagtctggt tcaacagata gtgctcttgc
     1141 aattccaatt ctttgccttt gtccacctga aaattcatgc ggatagcgat ttgcatgttc
     1201 tttatttaga cccactaatt ctaataattt ataaattttt tcatttcttt cagctttatt
     1261 ttccgccaat ttatgtatgt ctatcccctc acccacaata tccataactt taagtctagg
     1321 atttaatgaa gcataaggat cttgaaaaat catttgaatc cgtttgttta agttaaggct
     1381 atctcttcta gatgtaattt tgttaatatc ctttccttca aagaatatag tttcttctgg
     1441 tacctctaat aatctaataa tggactttcc aaatgtagat tttccacatc cagattctcc
     1501 tactatacca agcgcctcgc ctttatttat attaatgctc acgtcgtcaa ttgcttttat
     1561 ttcattattt ttatttttat taaaaatttt ggttaaattt ttaatttcaa caagtacatt
     1621 attgttcatt aaatgatact ccttctactt ttaacggtct ctcaaaatta taagaaagtt
     1681 ctttaaattt acacttaact gtttctggcg gaattacttt tggagccctg ctatctagta
     1741 accaagattt cacgaaatgt gtaggtgata attgaaacca tggcgcttca tatttaaaat
     1801 caacatttaa cgcatatgca cttcttcttg caaacgcatc accaataggt ggcttcaaca
     1861 agttaggtgg tgttcctgga attgcaatca attttgactt tgaattagac tctaatgtcg
     1921 gcattgaagc aagaagcccc catgtatacg gatgtttagg ataataaaat aattcttcta
     1981 cttttcctgt ctcaataatt tcaccacaat acataactga cactttgtct gcaatgttag
     2041 caacgactcc taaatcatga gtaataaaaa taattgcagt atttgttttc ttttgtaata
     2101 ttttcattaa atcaataatt tgcgattgca tagttacatc taatgcagtg gttggctcat
     2161 cagcaattaa taactttggt tcgcatgcta gagccatacc tattacaatc ctttgacgtt
     2221 gtccaccaga aaattgatgt gggtaatctt taaatcttaa ttcagcattt tttataccta
     2281 ctaaatctaa aatctctacg gcttttcctt tggcatcttt tctattcatt tttttatgta
     2341 tcatcaacgg ttccataatc tgtttaccta ttttcattgt agggtttagt gatgtcattg
     2401 gatcttgaaa aatcatggct atatcttttc ctcgtatctt agcaagttct ttttcatttt
     2461 tttcaactaa atcttcccaa aaaaacttca catatccatt tgtgattttt ccattttccc
     2521 cttggaacag tttagtaatt gctttagtag caactgattt acctgagcct gattctccaa
     2581 caattgctag tatttctccc tcatgtatgc aataatcaat tcctctcact gcattaacta
     2641 tgccatcttc aacttcgaaa gaaacttcta gattttttac ttccaatact tttttcatca
     2701 ttaaaacaca cccttattta cgcattttag gatcaaacgc atcgcgcagc ccatcactaa
     2761 ataaatagaa aaataaaatt aaaagactta aaattgttgc aggtatgaat aactcatgcg
     2821 gataaattaa tagcattgat ttaccatcat tcactaacga acctaatgat gtttgaggtg
     2881 caggtacacc aattccaata aaacttaaaa aggcttcaaa aaaaattgcg cttggaactg
     2941 tgaacatcga agtaactatt attgcaccta gtgtattagg taatatgtgt ttgaatatta
     3001 acctcaaatt tgaagcccct aatgcctttg acgcttgcac aaattctaaa tttttagttt
     3061 taagaaattc tcctcttact actctactca ttgaaatcca tccagtcact gacatagcta
     3121 atataattgt ccatattgat ggctcaaaga ttaatacgaa taaaattact ataattaaat
     3181 taggaatcga agctataatt tctataattc tttgcatgat aatatctaat ttgccaccat
     3241 aaaaaccaga tatagcacca aataaaactc caataaatat atctaaaata gcagcaacca
     3301 caccaataaa taatgatatt cgtgcacctt cccatgttct cgaccataaa tctcgaccca
     3361 actgatcggt tccaaataag taataatctt ttgaaccaac ctctttatat gtgtctttgc
     3421 cattaacatc tttaccatta aaaggcaaat aatcaacttt ttctaacaga ggtattttcg
     3481 aaggtaaatt tctatctgtg acattttgct cagtactatt atgctgagaa actgcgggtg
     3541 caaataaagc aaataaaatt atcattatta atccaatcat acctataaca gccaatttat
     3601 tttttcttaa ttgtgaccag ccatcttgcc aaaagctctt atttttaacg tttaaaaatt
     3661 cttgatttat tttatctttt ttaaattcaa aatccgttga agtcaattgc tttttatttt
     3721 catcaatcac cattatttct tcctccttgg atgcgtattc tt
//
LOCUS       DAHHRR010000166         3756 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.170, whole genome shotgun sequence.
ACCESSION   DAHHRR010000166 DAHHRR010000000
VERSION     DAHHRR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.170"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            86..868
                     /locus_tag="NBI42_002468"
     CDS             86..868
                     /locus_tag="NBI42_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1778854.1"
                     /translation="MGYLKRFALYISVMILIFAIAGCGKGNETKEGSKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTLLTSFSKSSKPGVIDDEGMVLYLNR
                     NTKKATGYYFVNKIYDDISKNQNEKKYRVELKNNKIVLLDNVEDEKLKQKIENFKFFS
                     QYADFKDLKNYQDGSITTNENVPSYEAEYKLNNSDSNVKKLREVYPITIKKAPILKVH
                     IDGDIKGSSIGYKKIEYKFSKVKDEETTLRDYLNFGPSDEDS"
     gene            916..1638
                     /locus_tag="NBI42_002469"
     CDS             916..1638
                     /locus_tag="NBI42_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1778855.1"
                     /translation="MGNLKSFALYISILILIVIVAGCGKSNETKEDSKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWVVRSEMIIQPKGESLTSRGMILYMNR
                     NTRTTTGYFSIDDIDSRKNLDERETEKKYPVKMINNKIIPTVKIKDEKLKKEIENFKF
                     FVQYGSFKGIENYENSDISYNSEGPIYSAKYKLKNDEYNVKELRKRYDIPTEKAPKLL
                     LKGSGDLKGSSVGYKEIEFIFL"
     gene            1756..2556
                     /locus_tag="NBI42_002470"
     CDS             1756..2556
                     /locus_tag="NBI42_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1778856.1"
                     /translation="MRFLKRVVLYISVIILTVFIIGCDSSSDTSEKSKEGSKETQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKPDELSNEGMFL
                     SLDRNTRTAKGYYFVRTFYRKDKLPYRKNYKVEMKNNKIILLDKVEDKKLKQKIENFK
                     FFSQYANLKELKNYSNGDVSINENVPSYDVKYKMSNKDENVKQLRSRYNIPTDKSPVL
                     KMHIDGNLKGSSVGDRRLEIDFSKRENSHLSVIDSLDYQAAETNKDDE"
     gene            2575..3345
                     /gene="lpl5"
                     /locus_tag="NBI42_002471"
     CDS             2575..3345
                     /gene="lpl5"
                     /locus_tag="NBI42_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HCD1778857.1"
                     /translation="MKSIKRIGLCISLLISSIFVTSCGGDNKITGDSKETQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKSDELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKIILLDKVEDPNLKKRIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEDRDISVIDYLSYKPARK"
     gene            3377..>3756
                     /locus_tag="NBI42_002472"
     CDS             3377..>3756
                     /locus_tag="NBI42_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1778858.1"
                     /translation="MEYLKRLALFISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDLYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELSNEGMVL
                     YLDRNTRTAKGHYFVKTFYNKGKFP"
BASE COUNT         1588 a          382 c          739 g         1047 t
ORIGIN      
        1 acgtaaaagg gaaatataca aaataattaa ttatttatat gaaaagagaa tataaatgaa
       61 gtataaaaca gagagacgtg aagcgatggg atatttaaaa aggtttgcat tgtacataag
      121 cgttatgatt ttaatatttg caatagcagg ttgtggcaaa ggtaatgaaa caaaagaagg
      181 atcaaaagaa gaacaaatta aaaagagctt tgcgaaaacg ttagatatgt acccaattaa
      241 gaatctcgag gacctatatg ataaagaagg atatcgagat ggtgaattta aaaagggcga
      301 taaggggacg tggactttac tcacaagttt ttcaaaaagt agcaaaccag gagtaataga
      361 tgatgaaggc atggtgctat atctaaatag gaataccaaa aaggcaacag gttattattt
      421 tgtaaataaa atttatgatg atattagcaa aaatcagaat gagaaaaaat accgtgttga
      481 acttaaaaat aataagattg ttcttttgga taatgtagaa gacgaaaaac ttaaacaaaa
      541 aattgaaaat tttaaatttt ttagtcagta tgccgatttt aaagatttaa aaaattatca
      601 agatggaagt ataacaacta atgaaaatgt accgagctat gaagcagaat acaaattgaa
      661 taatagtgat agtaatgtaa aaaaacttag agaagtttat ccaattacaa taaaaaaggc
      721 tccaatatta aaggtacata tagatggtga tataaaagga agttcaattg gatataaaaa
      781 aatagaatat aaattttcaa aagtaaaaga cgaagagaca acattaagag attatttaaa
      841 ttttgggccg tctgatgaag atagctaaag tttgttagat gtagaaatca ttctaaaaca
      901 gagagacgtg aaatgatggg aaatttaaaa agttttgcat tgtacataag tatcctgatt
      961 ttaatagtta ttgtagcagg ttgtggtaaa agtaatgaaa caaaagaaga ttccaaagaa
     1021 gaacaaatca aaaagagctt tgcgaaaacg ttagatatgt atccaattaa gaatctcgag
     1081 gatttatatg acaaagaggg atatcgagat ggcgaattta aaaagggaga taaagggaca
     1141 tgggttgtta gatctgaaat gataattcaa ccaaaaggtg aaagtttaac ttcaagaggc
     1201 atgatattat acatgaatag aaatactagg accacaaccg gttatttttc aatagacgat
     1261 atagattcaa gaaaaaacct tgatgagaga gaaacagaaa aaaagtatcc tgtgaaaatg
     1321 ataaacaata aaattattcc aaccgtaaag ataaaagatg aaaagttgaa aaaggaaatt
     1381 gaaaacttta agttttttgt tcaatatggg agttttaaag gaatagagaa ttatgaaaat
     1441 agtgacattt cctacaattc tgaaggtcct atttattccg cgaaatataa actgaaaaat
     1501 gatgaatata atgttaaaga attacgtaaa agatatgata taccaacaga aaaagctccc
     1561 aaattgttgt tgaaaggttc gggcgatttg aaagggtctt cagttggata taaggaaatt
     1621 gaatttatat tcctataaaa taaaaaagaa aatatatatt tttcagatgg attaaactta
     1681 attccaggtg actaaaaata acacaaaggt tgatggcgct caacaaatat taacatgata
     1741 gagagaagtg aaaaaatgag atttttaaaa agagtcgtat tgtacataag tgttattatt
     1801 ttgactgttt ttataatagg gtgtgatagt tcaagcgata cttcagaaaa atcaaaagaa
     1861 ggttcaaaag aaacacaaat taaaaagagc tttgcgaaaa cgttagatat gtatccaatt
     1921 aagaatctcg aggacttata tgataaagaa ggataccgtg atggcgaatt taaaaagggc
     1981 gacaaaggaa tgtggacgat atatacagat ttcgccaaaa gtaataaacc ggatgagcta
     2041 agtaatgaag gcatgttctt gagcttagat agaaacacac gcacagctaa ggggtactac
     2101 tttgtcagga cattttatcg caaggataaa ttaccttata gaaaaaatta taaagttgaa
     2161 atgaaaaata ataaaatcat tttattggat aaagtagaag ataaaaagct taaacaaaaa
     2221 atagaaaatt ttaaattttt tagtcaatat gcaaatctta aagaattgaa aaactacagc
     2281 aatggtgatg tctcaattaa tgagaatgtt ccaagttatg acgtgaaata taaaatgagt
     2341 aataaagatg aaaatgttaa gcaattaaga agtcgttata atattcctac tgataaatca
     2401 ccggtattaa aaatgcatat tgatggtaat ttgaaaggaa gttccgttgg agataggagg
     2461 ttagaaattg atttttcgaa acgtgaaaat agccatttat cagtaataga ttctttggat
     2521 taccaggcag cggaaactaa taaagatgac gaatgatgag gatggtgtgt aagaatgaag
     2581 tctataaaaa ggattggatt gtgcataagc ctgttgattt caagcatttt tgtaacatct
     2641 tgcggtggtg ataataagat cactggagat tcaaaagaaa cacaaatcaa aaagagtttt
     2701 gcgaaaacgt tagatatgta tccaattaag aatcttgagg acttatatga taaagaagga
     2761 taccgtgatg gcgaatttaa aaagggcgac aaaggaatgt ggactatata tacagatttt
     2821 gctaaaagca ataaatcaga cgaattagat gatgaaggta tggttttaaa tctggataga
     2881 aatactcgaa cggctaaggg atattatttt gttaagaaat tttatgaaaa ggataaatta
     2941 cctgatagaa aaaattataa agttgaaatg aaaaataata aaattatctt attagacaag
     3001 gtagaagatc caaatctaaa aaagagaata gaaaacttta aatttttcgg acaatatgca
     3061 aattttaagg atttggaaaa ttacaacaat ggcgacgtgt caataaattg gaatgttcca
     3121 agttatgacg tggaatataa aatgagcaat aaagatgaaa atgttaagca attaagaagt
     3181 cgttataaca ttcctactga taaagctcca atgttaaaaa tgcatattga cggtgactta
     3241 aaaggtagtt ctgttggata taaaagatta gaaatagact tttcaaaaga agatagggat
     3301 atttcagtca ttgattattt aagttataag ccagcgagaa aatagtatcg ataattaaat
     3361 attagggtgt gaaggaatgg aatatctaaa aaggcttgca ttgttcataa gtgttattat
     3421 tttgaccatt tttataatgg gttgtgatag tcaaagcgat actgcagaaa atccaaaaga
     3481 aggttcaaaa gaagcccaaa ttaaaaagag tttttcgaaa acgttagata tgtatccaat
     3541 taagaatctc gaggatctat acgacaaaga gggatatcgt gatggagagt ttgaaaaagg
     3601 tgacaaaggt atgtggacga tatatacaga tttcgccaaa agtaataaac cgggtgagct
     3661 aagtaatgaa ggtatggtct tatacttaga tagaaataca cggactgcaa agggacatta
     3721 ttttgttaag acattctata ataagggcaa attccc
//
LOCUS       DAHHRR010000167         3719 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.171, whole genome shotgun sequence.
ACCESSION   DAHHRR010000167 DAHHRR010000000
VERSION     DAHHRR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.171"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            52..402
                     /locus_tag="NBI42_002473"
     CDS             52..402
                     /locus_tag="NBI42_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HCD1778859.1"
                     /translation="MNDVVESLIYEVNSMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     LLNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTYG"
     gene            395..958
                     /locus_tag="NBI42_002474"
     CDS             395..958
                     /locus_tag="NBI42_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCD1778860.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQALLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            972..1316
                     /gene="gpsB"
                     /locus_tag="NBI42_002475"
     CDS             972..1316
                     /gene="gpsB"
                     /locus_tag="NBI42_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCD1778861.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNASSNNVDILK
                     RISNLEKAVFGK"
     gene            1379..1757
                     /gene="rnpB"
                     /locus_tag="NBI42_002476"
     ncRNA           1379..1757
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="NBI42_002476"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            1966..3111
                     /locus_tag="NBI42_002477"
     CDS             1966..3111
                     /locus_tag="NBI42_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCD1778862.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKAERKTIEN"
     gene            3195..3527
                     /locus_tag="NBI42_002478"
     CDS             3195..3527
                     /locus_tag="NBI42_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCD1778863.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
BASE COUNT         1378 a          492 c          714 g         1135 t
ORIGIN      
        1 atgacgaata cagtatattg tgataaatta aaaaatgtag gaggcgtcct catgaatgat
       61 gtagtcgaat cactaattta tgaagttaac agcatgcaac aaaattttga aaatgtgaaa
      121 tcacaacaac aagatcatga tttttaccaa actgtaaagc catatactga acatattgat
      181 agcttgctca atgagatcaa attacatcgt gaatttatta tagaagtacc ttatatgaat
      241 tcaaggaaat ttgagctact gattgctaac attgaacaac tttctgtcga atgtcatttt
      301 aagcgaacaa gtcgaaagtt atttatagaa aagcttaaaa gtgttcaata tgatttacaa
      361 aatatattag atggcgtaac taaagagggt acttatggtt aaaacagttt atgtaacagg
      421 ttataaatca ttcgaattaa acatttttaa agatgacgca cctgaagtac attatttaaa
      481 acaatttata aaacataaaa ttgaacaact gttggatgaa ggattagaat gggtgttaat
      541 acaagggcaa atgggtatag aattatggac tgcagaagtt gttattgaat tacaacgaac
      601 atatgattcg ttaaagtttg ctgtaattac accgttccaa ggacatacag aaaagtggaa
      661 tgaacataat caaagtaagt atgccaacat aattaagcat gcagattatg ttgatagtat
      721 ttttcatacg tcgtatcagg gtccttttca atttaaacaa gcagatcaat ttatgcttga
      781 acattcggat caagcattgc tcatttatga tgaggaacaa gaggcaagtc ctaagttctt
      841 caagcagatg ttagttgatt ttatggataa aacaaactat acttgtgata ttgtgacgtt
      901 cgatgaatta acagcattca tcaatgactt acagtggtct gaagatcaaa gtttctaatg
      961 aggtggaaaa aatgtcagat gtttcattga aattatcagc aaaagatatt tacgaaaaag
     1021 attttgaaaa aacaatggct cgtggctata gaagagaaga agtagatgca tttttagatg
     1081 acattattgc tgattatcaa aaaatggccg atatgaataa cgaagttgta aaattgtcag
     1141 aagagaacca taaacttaaa aaagaattag aagaattaag actacgtgta gcaacatcta
     1201 gacctcaaga taataaaagt ttttcttcaa ataatacaac aacaaacgca tcttcaaata
     1261 atgtagatat tttaaaacgt atttcaaact tagaaaaagc tgtatttggt aaataattac
     1321 taaatacaaa agtttaactg tcttactaat aatgactatg ttataatttt aaaagtgata
     1381 ttttgggtaa tcgctatatt atatagagga aagtccatgc tcacacagtc tgagatgatt
     1441 gtagtgttcg tgcttgatga aacaataaat caaggcatta atttgacggc aatgaaatat
     1501 cctaagtctt cgatatggat agagtaattt gaaagtgcca cagtgacgta gcttttatag
     1561 aaatataaaa ggtggaacgc ggtaaacccc tcgagtgagc aatccaaatt tggtaggagc
     1621 acttgtttaa cggaattcaa cgtataaacg agacacactt cgcgaaatga agtggtgtag
     1681 acagatggtt atcacctgag taccagtgtg actagtgcac gtgatgagta cgatggaaca
     1741 gaacatggct tatagaaata tcactactag tttagctctc ctagatgatg gagagctttt
     1801 ttcatgaaaa gaacacttaa aattaacgcc ttgtcttgat ataatgacac tgccttgttt
     1861 taaaataata agcgaatgcg ttaatgtatc agcgattaaa tttgttggaa atgtataaaa
     1921 aacacaagct aagaataaaa tacctgtaca aaaggagaat catatatgtt tcaattactt
     1981 gcagtttgtc cgatgggatt agaagctgtt gttgctaggg aaattcaaga attaggctat
     2041 gaaacaaatg ttgaaaatgg ccgtatattt tttgaaggag acgcaagtgc aattgtaaag
     2101 gcgaatttat ggttgcgcac agcagaccga atcaaaattg ttgttggacg ttttaacgca
     2161 acaacgtttg acgaattatt cgaacaaacc aaagcgctcc cttgggaatc tattattgat
     2221 aaagaaggta acttcccagt tcaaggtaga agcgttaaat caacactaca tagtgtgcca
     2281 gattgtcagg cgataactaa gaaagctatt gttgaacgat tacgtcgcgc atataacgaa
     2341 aaaggttggt taaatgaatc aggtgcaaaa taccctgttg aagttgccat tttaaaagat
     2401 aatgtattat tgaccatcga cacatcaggt tctggtttga acagacgtgg ttatagatta
     2461 gcacaaggtg aagcaccaat taaagaaacg ttggcagcaa gtttaatccg tcttgcaaac
     2521 tggaaaggtg atacaccttt aattgatcca ttttgcggtt cgggtacaat agctatagaa
     2581 gcttgtttaa ttgctcaaaa tatcgcacct ggttttaatc gagagttcgt atcagagcaa
     2641 tggaacatca tgccagcaaa tatttatgat gattaccgtg atgaagcgga taagatggct
     2701 gattatgata aagaaatcga agtatatgct tctgatatcg atcctgaaat ggtagagatt
     2761 gctaagcgta acgctgaaga agttgggttg tctgatatta ttaaatttag tgtaaaagat
     2821 gtcaatacat taacaattga tacagaagaa ccggtggcgt taattggaaa tcctccatat
     2881 ggtgaacgta ttggtgatcg tgaagaagtt gaagaaatgt accgttatat tggtaaacta
     2941 atgaaacaac atccattttt atctacatac attttaacaa gtaataaaga atttgaatat
     3001 ttagtagatc gtaaagcaac gaagcgacgt aagttattta atggatatat agaatgtact
     3061 tattatcaat actggggtaa aaaggctgaa cgtaaaacta ttgaaaacta aagtgtttct
     3121 aatgtgtgac taaaattagt aataattaag ttctcatgat aataggtatt tttgaaaaat
     3181 ggaggagtct ataaatgggt aaaaaaatgg gtctaggttt atctatagca ttggttgtta
     3241 ttggtattgc cgttgtatgt ttaatgattt tttctagtca aaaaacgact tattttggtt
     3301 atatgaatag taatacaaat gcagaaaaag ttgtcagtga aaaagatgga ttagtcaaac
     3361 ataatatcaa agtagaacca tcaaatgatt ttaaaccgaa aaaaggagac tttgtaaaat
     3421 tagtttctaa agatgatggg aagacatttt ataaacaaga aattgttaaa catgatgacg
     3481 tcccacacgg tttaatgatg aaaattcacg acatgcatat gaattaataa aaagcatcta
     3541 taacgtaatt tggaagaagt aaagtaatct tcttatacgt tttagatgct tttttatcag
     3601 ttcgatttat tatagaaccc gttttaagta attatagagt cgagaatcat tttgtaaatt
     3661 tcgtcagttc aaaactttaa gtgcattatt gtttagcacg acgatcttta atatataat
//
LOCUS       DAHHRR010000168         3643 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.172, whole genome shotgun sequence.
ACCESSION   DAHHRR010000168 DAHHRR010000000
VERSION     DAHHRR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.172"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            263..697
                     /locus_tag="NBI42_002479"
     CDS             263..697
                     /locus_tag="NBI42_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD1778864.1"
                     /translation="MKLKSFITVTLALGMIATTGATVASNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(954..1139)
                     /locus_tag="NBI42_002480"
     CDS             complement(954..1139)
                     /locus_tag="NBI42_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCD1778865.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            1434..2375
                     /locus_tag="NBI42_002481"
     CDS             1434..2375
                     /locus_tag="NBI42_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCD1778866.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASVKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSEGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKLKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            2387..3631
                     /gene="fabF"
                     /locus_tag="NBI42_002482"
     CDS             2387..3631
                     /gene="fabF"
                     /locus_tag="NBI42_002482"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCD1778867.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
BASE COUNT         1304 a          518 c          707 g         1114 t
ORIGIN      
        1 aacaagggat ttaatttata tattaggtac ggcgttcgct ataatgcaaa gaagtaatta
       61 aatttaagga atgtaaactt agttattgta atgtgaattt atttgaaaaa atagaaagta
      121 ttaacaatta tagcttttac attaattaaa atttattttt aaaagcaagt aaacaattta
      181 catacttata atttttggaa cttttagatt tgtgttatat tgattttgta agatacttta
      241 actcacaaag gagagagatt atatgaaatt aaaatcattt ataactgtaa ctttggcact
      301 gggcatgatc gcaacgactg gcgctactgt ggcaagtaat gaggtatctg cagcagaaaa
      361 ggacaaacta ccggcaactc aaaaagctaa agaaatgcaa aatgttccat atacaattgc
      421 agtagatggc attatggctt tcaatcaatc ttacttaaat ttaccaaaag atagccaatt
      481 atcatattta gatttaggaa ataaagttaa agctttatta tatgatgaac gtggtgtaac
      541 acctgagaag attcgaaatg caaaatctgc cgtttacacg attacttgga aagatggcag
      601 taaaaaagaa gtagatttaa agaaagatag ttatacagca aacttgtttg attcaaattc
      661 aattaaacaa attgatatta atgtaaaaac taaataaaca cttaaaatta tcaaattcaa
      721 catgaagcat ttaattttac agtgatgatt ataatataat tgccttgata taaagattac
      781 tcgtaaatga catctttgta ttaaggcttt ttctaaattt aaaagtgatg ggttagaggt
      841 cattgagctt taaaatattc aaaatacaaa acatcaatgg ccaaaaataa aagccgcctt
      901 tatctgggca gcttcaataa taagaaagac atatttcatt ttatactaaa tagttattgt
      961 gatgaatctt tcggcggttt aattactgca gcaaaaattg ctgtgaaaat cgtgaacaat
     1021 actgccatga taattggatt cactacattt aagctgtctc cacctactag gctattaagt
     1081 acaaagttaa ccatttgcat taataataat gcccaaaaga atgttacgag gtgtttcatg
     1141 tcattctacc tccactttaa ttatatatat tttattttaa gtgaaagtta gaaatttgta
     1201 tagtaacatc tcatatattt tgaccatatt atacagttta aataaatgat tttatctgaa
     1261 tggctattct aaattaagcg cattaaaacc aatttcatac tgaaatttga cgataataaa
     1321 gcattaaaat tttattaact agtcaatatt cctacctctg acttgagttt aaaaagtaat
     1381 ctatgttaaa ttaatacctg gtattaaaaa ttttattaag aaggtgttca actatgaacg
     1441 tgggtattaa aggttttggt gcatatgcgc cagaaaagat tattgacaat gcctattttg
     1501 agcaattttt agatacatct gatgaatgga tttctaagat gactggaatt aaagaaagac
     1561 attgggcaga tgacgatcaa gatacttcag atttagcata tgaagcaagt gtaaaagcaa
     1621 tcgctgacgc tggtattcag cccgaagata tagatatgat aattgttgct acagcaactg
     1681 gagatatgcc atttccaact gtcgcaaata tgttgcaaga acgtttaggg acgggcaaag
     1741 ttgcctctat ggatcaactt gcagcatgtt ctggatttat gtattcaatg attacagcta
     1801 aacaatatgt tcaatctgga gattatcata atattttagt tgtcggtgca gataaattat
     1861 ctaaaataac agatttaact gaccgttcta ctgcagttct atttggagat ggtgcaggtg
     1921 cggttatcat cggtgaagtt tcagaaggca gaggtattat aagttatgaa atgggttctg
     1981 atggcactgg tggtaaacat ttatatttag ataaagatac tggtaaactg aaaatgaatg
     2041 gtcgagaagt atttaaattt gctgttagaa ttatgggtga tgcatcaaca cgtgtagttg
     2101 aaaaagcgaa tttaacatca gatgatatag atttatttat tcctcatcaa gctaatatta
     2161 gaattatgga atcagctaga gaacgcttag gtatttcaaa agacaaaatg agtgtttctg
     2221 taaataaata tggaaatact tcagctgcgt caataccttt aagtatcgat caagaattaa
     2281 aaaatggtaa actcaaagat gatgatacaa ttgttcttgt cggattcggt ggcggcctaa
     2341 cttggggcgc aatgacaata aaatggggaa aataggagga taacgaatga gtcaaaataa
     2401 aagagtagtt attacaggta tgggagccct ttctccaatc ggtaatgatg tcaaaacaac
     2461 atgggagaat gctctaaaag gcgtaaatgg tatcgataaa attacacgta tcgatactga
     2521 accttatagc gttcacttag caggagaact taaaaacttt aatattgaag atcatatcga
     2581 caaaaaagaa gcgcgtcgta tggatagatt tactcaatat gcaattgtag cagctagaga
     2641 ggctgttaaa gatgcgcaat tagatatcaa tgaaaatact gcagatcgaa tcggtgtatg
     2701 gattggttca ggtatcggtg gtatggaaac atttgaaatt gcacataaac aattaatgga
     2761 taaaggccca agacgtgtga gtccattttt cgtaccaatg ttaattcctg atatggcaac
     2821 tgggcaagta tcaattgact taggtgcaaa aggaccaaat ggtgcaacag ttacagcatg
     2881 tgcaactggt acaaactcaa tcggagaagc atttaaaatt gtgcaacgcg gtgatgcaga
     2941 tgcaatgatt actggtggta cggaagctcc aattactcat atggcaattg caggtttcag
     3001 tgcaagtcga gcgctttcta caaatgatga cattgaaaca gcatgtcgtc cattccaaga
     3061 aggtagagac ggttttgtta tgggtgaagg tgctggtatt ttagtaattg aatctttaga
     3121 atcagcacaa gctcgaggtg ccaatattta tgctgagata gttggttatg gtactacagg
     3181 tgatgcttat catattacag cgccagctcc agaaggtgaa ggcggttcta gagcaatgca
     3241 agctgctatg gatgatgctg gtattgaacc aaaagatgta caatacttaa atgcccatgg
     3301 tacaagtact cctgttggtg acttaaatga agttaaagct attaaaaata catttggaga
     3361 agcagctaaa cacttaaaag ttagctcaac aaaatcaatg actggtcact tacttggtgc
     3421 aacaggtgga attgaagcaa tcttctcagc gctttcaatt aaagactcta aagtcgcacc
     3481 gacaattcat gcggtaacac cagacccaga atgtgatttg gatattgttc caaatgaagc
     3541 gcaagacctt gatattactt atgcaatgag taatagctta ggattcggtg gacataacgc
     3601 agtattagta ttcaagaaat ttgaagcata attataaaag agc
//
LOCUS       DAHHRR010000169         3634 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.173, whole genome shotgun sequence.
ACCESSION   DAHHRR010000169 DAHHRR010000000
VERSION     DAHHRR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.173"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(166..1455)
                     /locus_tag="NBI42_002483"
     CDS             complement(166..1455)
                     /locus_tag="NBI42_002483"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCD1778868.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLYGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLNRLGERL
                     ENGLLELIEKHQITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            1536..1991
                     /gene="bcp"
                     /locus_tag="NBI42_002484"
     CDS             1536..1991
                     /gene="bcp"
                     /locus_tag="NBI42_002484"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCD1778869.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            1997..2947
                     /locus_tag="NBI42_002485"
     CDS             1997..2947
                     /locus_tag="NBI42_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCD1778870.1"
                     /translation="MKVVGLNRMREVETELQQRFSDVDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKHEFDEIYTIESLESTLPNADIVINALPETQETIHLLKKNHFELMK
                     DEALFINIGRGTIVKEALLIEVLKSRVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDTKKGY"
     gene            3044..3490
                     /gene="perR"
                     /locus_tag="NBI42_002486"
     CDS             3044..3490
                     /gene="perR"
                     /locus_tag="NBI42_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HCD1778871.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
BASE COUNT         1317 a          543 c          607 g         1167 t
ORIGIN      
        1 gcgatgtaaa actttagtca gagattaaaa gagtataaat ttgtaaaata aaaactcaca
       61 tagtgacata tcaagttaaa cgttaatagt taacgatata aaatgaatct actatgtgag
      121 catttgtttt attttaattc aatttaaaat atacttcctt aaaagttatt tcatttgact
      181 aaaagcatag tctgcagctt ttaaagtttg tttaatatct tcttctgtat gttcagttgt
      241 taagaaccaa gcttcaaact tagaaggtgc taaattgata ccttgattta acattaattt
      301 gaaaaattta ccgaacgctt cgccgtcaga atgttcaact tgatcataat gtgtgacttt
      361 ttcatctgta aagtacaatg ttaaagatcc gtatatacga ttaattgtag ctgtaatttg
      421 atgtttttca attaactcaa gtaaaccatt ctctaaacgt tctcctagac gatttaactt
      481 ttcgtaaaca ccgtcttgtt ctaacacttc taataatgca atacctgctt tcatagataa
      541 tggattacca gccatagtac cggcttgata tgcaggtcct agaggtgcta cttgttccat
      601 aatatcttga cgtccaccat agcctccaat tggtaaacca ccgccaacga ttttaccaaa
      661 tgcagttaaa tcagggataa cacctaataa atcttgagcg gcaccgtaat ggaaacggaa
      721 tgcagtaatt acttcatcat aaatcactag tgtcccgttg ttatgtgaaa tttcattaac
      781 ctcttctaaa aatccaggtt gaggcattac cattccaaag ttaccaacaa ttggttctac
      841 taatactgcg gcaatttcat caccccaaaa ttcaattgct tctttatatg cgttaatatc
      901 attgaaagga acagtaatga cttcacgtgc gacgctttct ggaacaccag ctgaatctgg
      961 agaaccgagc tgagatgggc cgctacctgc tgcaaccaat actaaatcag aatggccatg
     1021 ataagatcca gcaaatttta taattttatt tcttttagta tatgcacgtg caacacgaat
     1081 tgttgtcatg actgcttctg ttccagaatt tacaaagcga attttctcaa gagatggaat
     1141 tgcatcacgt aattttttgc tgaattcaat ttctagttca gtcggtgtac catataaaac
     1201 acctttagca gcttgttctt gaattgcttt agtaatatga ggatgtgcat gccccgtaat
     1261 aattggaccg tatgcttgaa ggtaatcaat aaatttattg ccatcgacat catataaata
     1321 tgcaccgtgt ccttctttca ttacaacagg tgcaccgcct cctacagctt tataagaacg
     1381 agaaggggaa ttgacaccgc ctagaatata ttcgtttgaa agttgttgta aacgttcact
     1441 ttcgctaaaa ttcatttata tcaacctctt ttaatttaat attttcatct aatatcgtat
     1501 cataaaatta ttataatgaa gaaaaaggtg attatatgtt gcaaaaagga gaacaatttc
     1561 caatatttaa attagaaaat caggacggaa ctgtcattac aaatgataca ttaaaaggta
     1621 aaaaggcgat tatatatttt tatcctagag ataatacacc tacttgtacc acagaagctt
     1681 gtgactttag agacaattta gaaatgttca atgatttaga tgttgcagta tatggtataa
     1741 gcggtgattc aaagaaaaaa caccaaaatt ttattgaaaa acacggattg aatttcgatt
     1801 tattagtaga tgaagatttt aaattagcta aagaaactgg cgtatatcag ttaaaaaaat
     1861 catttggcaa agaaagtatg ggcattgtaa gaacgacttt tataatagat gaacaaggta
     1921 aagtattaga tgttatcgag aaggttaaag ttaaaacaca aatagaagaa cttaaaaaca
     1981 ttttggggtg acatatatga aagttgttgg gttgaatcgt atgcgtgaag ttgaaactga
     2041 attacaacaa cgcttttcag atgtagattt taaattttat aaaaaagcat cagaaatacc
     2101 tgagagcgac ttggctgatt tagatatatt agttggttat gatggcggta tcaatgaggc
     2161 atttttacga cgttgcccga atttaaaatg gatcgcatgg tttgcaaccg gtgtaaatac
     2221 attaccgtta gattatattg cagatcacgg catactttta actaatggaa aaggtgttca
     2281 agctaaacaa ttatctgaat acattttagc tttcatttta gatgattata aaaagatgaa
     2341 actatcatat gataaccaac gacaacatat atatgattcg aaaataactg gtaaacgcct
     2401 ttcaggacaa acagttttat ttttaggtac aggtgcaatt gctactagaa ctgcgaagtt
     2461 agcaaaggct tttaatatga atttaattgg tctgagtaag tcaggtcaga acaaacatga
     2521 gtttgatgag atatatacta tcgaatcact tgaaagcaca ttaccaaatg ctgacattgt
     2581 tataaatgct ttaccagaaa cgcaagaaac gattcattta ctaaagaaaa atcattttga
     2641 attaatgaaa gatgaagcac tttttataaa tataggacga ggtaccatag ttaaagaagc
     2701 gctcttaata gaagtattaa aaagtagagt tattcgacat gcgtatttag atgtgtttga
     2761 aaatgaacct ttgaaaccta atcatgaatt atatgaattg gataatgtaa ctataacagc
     2821 gcatataact ggtaatgatt atgaagcaaa gtatgactta ttagatattt ttaaaaacaa
     2881 tctagttaat tttctcaata agaatggtct aattgagaat gaagtggata ctaaaaaagg
     2941 ctattaaacg aaatcgtcat gtaaatgttg acacgcgcgc aatactacag ttatatttat
     3001 agtaagtaat aataattatt atataagaaa gatggtgata tagatgagtg ttgaaataga
     3061 atcaattgaa catgaattag aagaatcaat tgcatcattg cgacaagcag gcgtaagaat
     3121 tacacctcaa agacaagcaa tattacgtta tttaatttct tcacatactc atccaacagc
     3181 tgatgaaatt tatcaagcac tttcacctga ttttccaaat ataagtgttg cgacaatata
     3241 taataactta agagtgttta aagatattgg aattgtaaaa gaattaacat atggagactc
     3301 atcaagtcga ttcgacttta atacacataa tcattatcat attatatgtg aacaatgtgg
     3361 taagatcgtt gattttcaat atccacagtt aaatgaaatt gaaagattag ctcagcatat
     3421 gactgacttt gacgtaacac atcatcgaat ggaaatttat ggagtttgta aagaatgcca
     3481 agataaataa tttaactttg gtagtatgac aaattaaaaa atcgttagct tacttcatat
     3541 aagtaagcgt aatatttaag aagttaaacg acatgaaagt tgtttaactt cttttttatg
     3601 ctcattctga aaaatattta aatagaagaa aaga
//
LOCUS       DAHHRR010000170         3607 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.174, whole genome shotgun sequence.
ACCESSION   DAHHRR010000170 DAHHRR010000000
VERSION     DAHHRR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.174"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(134..274)
                     /locus_tag="NBI42_002487"
     CDS             complement(134..274)
                     /locus_tag="NBI42_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCD1778872.1"
                     /translation="MKKLLNKVIELLVDFFNSIGYRAAYINCDFLLDEAEVPKELTQL
                     HE"
     gene            complement(278..841)
                     /locus_tag="NBI42_002488"
     CDS             complement(278..841)
                     /locus_tag="NBI42_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HCD1778873.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFIFTLITNISFYLIRRYAHGAHAPSSFWCYIESITLFIVLPLLVLHF
                     HINETLMMFLALISVGVVIKYAPAATRKKPIPARLVKQKRYFSIIISTILFIITLFVK
                     EPYTQFIQLGIIIQAITLLPIYYSKED"
     gene            1077..1211
                     /locus_tag="NBI42_002489"
     CDS             1077..1211
                     /locus_tag="NBI42_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HCD1778874.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
     gene            complement(1811..2596)
                     /locus_tag="NBI42_002490"
     CDS             complement(1811..2596)
                     /locus_tag="NBI42_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HCD1778875.1"
                     /translation="MKVQIYQLPIIFGDSSKNETQITQWFEKNMNTEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNSEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
     gene            complement(2959..3585)
                     /locus_tag="NBI42_002491"
     CDS             complement(2959..3585)
                     /locus_tag="NBI42_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCD1778876.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
BASE COUNT         1259 a          558 c          504 g         1286 t
ORIGIN      
        1 aatctgattt tttgtatctt attccactaa taaatgcaga tataaagaac attaatgaaa
       61 cttgaacaat tacaaataaa acgtaattat aatcatttaa tgcttccact tactatcaca
      121 ctttctctta tagttattcg tgtaattgag ttaattcttt tggtacttca gcttcgtcca
      181 ataaaaaatc acaatttata tacgcggctc tgtaaccgat gctgttgaaa aagtcaacta
      241 aaagctcaat aactttgttg agtaattttt tcataattta gtcctccttt gaatagtata
      301 ttggtagtaa tgtaatagct tgtataataa tacccagttg aataaattgg gtatatggtt
      361 cttttacaaa aagtgtgata atgaaaagaa ttgtactaat tataattgaa aaatatcttt
      421 tttgctttac aagcctcgct gggataggct tcttcctagt agctgcaggt gcatatttaa
      481 tcactactcc aacacttatt aatgctaaaa acatcattag tgtttcatta atatgaaaat
      541 gtaatactaa tagaggtagc acaataaaaa gtgtaatgct ttctatataa caccaaaaag
      601 atgaaggtgc atgtgcacca tgtgcatacc ttctaattag ataaaatgat atattagtaa
      661 ttaaagtgaa gataaaaatg tttaaaatat aggcaatagt atacataaca attaatttac
      721 ctatattttt agctaagacc tgcatcccta aacgtacttg caaaaattga atatgatcta
      781 agttatttct cttttgaaga tacgtggcaa actggtcaat tttattatca aaataattca
      841 attttacacc actctcctca ctgtcattat acgatttagt acaatctttt atcattatat
      901 tgcctaactg taggaaataa atacttaact gttaaatgta atttgtattt aatattttaa
      961 cataaaaaaa tttacagtta agaataaaaa acgactagtt aagaaaaaat ggaaaataaa
     1021 tgcttttagc atgttttaat ataactagat cacagagatg tgatggaaaa tagttgatga
     1081 gttgtttaat tttaagaatt tttatcttaa ttaaggaagg agtgatttca atggcacaag
     1141 atatcatttc aacaatcggt gacttagtaa aatggattat cgacacagtg aacaaattca
     1201 ctaaaaaata agatgaataa ttaattactt tcattgtaaa tttgttatct tcgtatagta
     1261 ctaaaagtat gagttattaa gccatcccaa cttaataacc atgtaaaatt agcaagtgag
     1321 taacatttgc tagtagagtt agtttccttg gactcagtgc tatgtatttt tcttaattat
     1381 cattacagat aattatttct agcatgtaag ctatcgtaaa caacatcgat ttatcattat
     1441 ttgataaata aaattttttt cataattaat aacatcccca aaaatagatt gaaaaaataa
     1501 ctgtaaaaca ttcccttaat aataagtatg gtcgtgagcc cctcccaagc tcgcggcctt
     1561 tttttattgt aataaagaag ggaagagtta atcctcatta tgagatccac cgttaaaata
     1621 tatgaataag tctaatgttg gaaaaggtca aaaaataatt aaattaatta agaaaatcat
     1681 tcatttgcta agggcgaaat tggttcttat tgagttatct attataaaaa aataaacata
     1741 gacttatgaa aaatctctca taaatctatg tttagtcatg acatgtgtta attattattt
     1801 cgggcgcttc ttatttatac aaatctaatt taatactttt aaatacaggt atattttcgc
     1861 gttgctgttc tacttcattt aagtttaaat ctacagtcaa aatatctgcg gattcattta
     1921 attctccaac taaatctcca tttggattta taactatcga atgaccagca tattctgagt
     1981 taccatcgaa tccagtgcta ttagttccaa tgacaaacat attattttca attgcacgtg
     2041 cctttagtaa cgaatgccaa tgttgaagtc gtgacatagg ccattgcgcc acataaaatg
     2101 caattttagc accactacga gcaggatatc ttaataattc tggaaaacgt aaatcataac
     2161 agataagttg ggtcacataa gtaccgtcag acaattgaaa gggttcagct acgttttcgc
     2221 cagcggttaa aaattcatgc tctcttaaca taggaactaa atgaactttg tcgtattcat
     2281 taatcagctg gccactttta ttcacactaa aagctgtatt aaatatttga ttgtttctaa
     2341 tgttagaaac tgacccagct acgatatcga ctttatattt ttcagctaaa tgtttaataa
     2401 atgaaaaact ttgtcctaga ttattatctg ctttttcatt taaatgctct aaatcatagc
     2461 cattattcca catttcaggt aaaacgacga catctacttc agtattcata tttttttcga
     2521 accattgcgt tatttgagtt tcatttttag aactatctcc aaaaataatc ggtaattgat
     2581 aaatttggac tttcataaca tcacatcctt gatagatctt atatataact tactaaaagt
     2641 tatgttgaaa cgcaaaaaaa cgagcccagg acataaaatc aaagtcctag gctctacaat
     2701 gttatattga cagtagttga tggggcccca acatagagaa attggaacac caatttctac
     2761 agacaatgca agttggggtg ggctctaaca taaagaaata ctttttcttt agaaattagt
     2821 atttcttata catgagtttt actcatgtat tcctattctt aagtatacat tagcagcggc
     2881 taatgtgtag gaaactaata cataatgaat aactaatgat tctttatcat ttctgtccca
     2941 ttcctaacaa tatattgatt atttttttat tacgaaacga tcttccactg gattaaatgt
     3001 tttttcgcca gcagcttcac gaatatcacc aaaaggcatt tgagcaataa gtttccaact
     3061 tttaggaata ttaaattcat ttgaagtcat ctcatcaaca agtggattat agtgctgtaa
     3121 tgaagcacct atgcctttag tagctaatgc agtccaaatt gcaaattgat gcatggcatt
     3181 tgtttgagtt gaccatattg caaaattatc atagtagttt ggcatttgtt cttgtaaacc
     3241 acttacaaca tcttgatctt cataaaacaa aattgtaccg tatgaatgtt tgaagttatc
     3301 aattttttgt tcagttggct cgaaatcacg attctctccc atgacttctt ttaaaattgc
     3361 ttttgtgtta tcccaaaatt tattattgtt gtcatttaat aagagaacaa ttctagttga
     3421 ttgagaatta aatgatgaag gaacatgttt aactgcatgt gcaatcattg attctaattc
     3481 gtcatcgcta attgatattg aatctttcaa attatatatt gaacgtcttt cttccattgc
     3541 attgtcaaaa gtcattgctt ttttatcttt tttaaataaa cccataatta ttgctccttc
     3601 tttagta
//
LOCUS       DAHHRR010000171         3604 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.175, whole genome shotgun sequence.
ACCESSION   DAHHRR010000171 DAHHRR010000000
VERSION     DAHHRR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.175"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            285..686
                     /locus_tag="NBI42_002492"
     CDS             285..686
                     /locus_tag="NBI42_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1778877.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(806..1369)
                     /locus_tag="NBI42_002493"
     CDS             complement(806..1369)
                     /locus_tag="NBI42_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1778878.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1366..2319)
                     /locus_tag="NBI42_002494"
     CDS             complement(1366..2319)
                     /locus_tag="NBI42_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCD1778879.1"
                     /translation="MGNHFQYSFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVAVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2429..>3604
                     /locus_tag="NBI42_002495"
     CDS             2429..>3604
                     /locus_tag="NBI42_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1778880.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     "
BASE COUNT         1126 a          546 c          651 g         1281 t
ORIGIN      
        1 gtaatattga tatattttct ttgtatataa attataaaat taatatgtaa tagagtgatt
       61 tgttttatgt actattatct tatttctaaa tattaactct attgattatt ggtttttata
      121 cttatttaat tttattcaac tttgacaatt gaatagaaag caagtttatt tacacttgta
      181 gttttaagca taagttagca catacaagtt ttgggattgt tgggatgttt gttaatactt
      241 gtatggtggc taaatatgtg atcattaatt gggagatgtt tagcatgaaa aaagtaaata
      301 acgatactgt atttgggatt ttgcaattag aaacactttt gggtgacatt aactcaattt
      361 tcagtgagat tgaaagcgag tacaaaatgt ctagagaaga aattttaatt ttactaactt
      421 tatggcaaaa aggttctatg acgcttaagg aaatggacag atttgttgaa gtcaaaccgt
      481 ataagcgtac tagaacgtat aataatttag ttgaattaga atggatttac aaagagcgtc
      541 ctgttgacga tgaaagaaca gttattattc atttcaatga aaagttacaa caagagaaag
      601 tagagttgtt gaatttcatc agtgatgcga ttgcaagtag agcaacagca atgcaaaata
      661 gtttaaacgc aattattgct gtgtaagttt aatagcataa aaagaggttt tcattaagtt
      721 gaaaacctct ttttgttgtt ggtattaatt tttcaaatgt tgactactca atcctaaatt
      781 ataaatagta tagcgcggca aatgcttaag aaattttttc tatggcacaa atgaatggag
      841 catgattacg ttggtttaaa aattgatatt gcaaaacttg cgcatgcttt tgatccaaag
      901 tactcaagta atcaagcaat gcatgcttct caatttgtcc ttcgctatga ccatgatata
      961 taacaagtac aataatacct tcaattgaca ttaacgatag caatgaatta atagcttgga
     1021 ttgtcgtgtc aggctttgtc acgatagatt tatcaccttt aggcaaataa cctaggttaa
     1081 agatggctgc atcaatatga cctttatgtg catcatttat atgatgttca atattttcat
     1141 gtccatcttt tattaaagaa acatgattga aatccttaac tttatcacgt gtattttcca
     1201 aagctaaatc ttgaatgtcg aaaccataaa catgtccttc tggtacttgt tcggctaaaa
     1261 ataaagtgtc attgccgtta ccgcaagttg cgtctacaac aatactttct ggtgttatat
     1321 gttgtttaat aagtgttttt gaaaaaggga gtatacgttc taatttcatt gcttcacctt
     1381 agacttgtaa cgcaagcctt gataagaatt tctacgtgct aattcagcat cgatgccatt
     1441 taatacttcc catttattaa cactccacat tggaccgacc atgatatcta ttggaccatc
     1501 accggtaatt cggtgaacga tcatttcagg gggaatcact tctaattgat ccacaactag
     1561 gtttgtgtat tcttcttgag tcataaaagt taataaacct ttatcgtatt gttttaccat
     1621 cggtgtacct tttaacaaat gaagtaaatg aattttaata ccttgtacat ccatttgtgc
     1681 aacttctttg gcagtagcca tcatcatgtc atagtcttcg ccaggtaagc cattaatgat
     1741 gtgtgtacat acattgatat tatgcttacg taattttgcc acaccatcat aataagtttt
     1801 catatcatgg gcacgattga ttaaatcaga tgttgattga tggattgttt gtagtcctaa
     1861 ttcaacccat aagtatgttc gttgattcaa atctgctaaa tattcgacaa catcgtctgg
     1921 taaacagtca ggacgcgtac caatagataa tcccacaaca cccggttctt taagtacagg
     1981 ttcgaatttt tcttttaata ctgcaactgg tgcatgtgta tttgtaaatg cctgaaaata
     2041 agcaatatat tttccttcgt gccatttctc atgcatcttt tccttaattt ctttaaattg
     2101 tactgcgatt gaatctgcac gattacctgc aaagtctccg ctacctgcag cagaacaaaa
     2161 tgtacatcca ccatgtgcta cagtgccatc gcggttagga cagtcaaacc cgccatccaa
     2221 tgcaacttta aatatttttt gtccaaattt attttttaaa tggtaattcc atgtgtgata
     2281 acgtttgttt tcaaaagagt attggaaatg attgcccata tgtcattttc ctttctataa
     2341 aaaaagagtt ctaagtacag attttaacat attttaatgt tatagtgttt attatagttt
     2401 gacaaaaaag agagaggaac tatgaaatat gaatatacct aaatcagtct ggtggctagt
     2461 aattggcatg gcgttaaata ttactggttc cagttttttg tggcctttaa atacaattta
     2521 tatgaaacaa gaacttggaa aaagtttaac tgttgctggt ttagtgctaa tgataaattc
     2581 atttggcatg gttattggaa acttattagg tggttcacta tttgataaat taggtggata
     2641 caagacgatt ttaattggaa ctttcacttg tctttgtagt acaacgctac ttaatttctt
     2701 tcacgggtgg ccttggtatg ctgtatggct tgtaatgtta ggctttggtg gtggaatgat
     2761 tattcctgcg atatacgcaa tggctggagc agtgtggccg aatggcggaa ggcaaacgtt
     2821 taatgcgata tacttagcac aaaatattgg tgttgctgtc ggcgcagcaa tgggcggctt
     2881 tgtcgcagaa tttagcttta actatatctt tttagccaat cttattatgt atgttgtgtt
     2941 tgcacttgtc gcggtaacgc aatttaatat tgaaattaat gcgaaagtta aatatccaac
     3001 tcatttagat attactggta aaaagaataa agcaagattt atttcattag tactaatttg
     3061 tgcaatgttt gcaatttgtt gggttgcgta tattcaatgg gagtctacaa tcgcatcatt
     3121 tacacaatct attaatattt caatggcaca atatagtgtt ttatggacaa ttaacggaat
     3181 aatgatttta gtagcacaac cattaattaa accgattctc tatctgttaa aaggaaactt
     3241 aaagaaacaa atgtttgtcg gcatcatcat ttttatgttg tcgttctttg tcacgagctt
     3301 tgccgaaaac tttacaatat ttgttgtcgg tatgattatt ttaacttttg gagaaatgtt
     3361 tgtatggcca gcagttccaa ctatagccaa tcagttagcg ccagatggta agcaaggaca
     3421 gtaccaaggt tttgtgaatt cagctgctac agtaggaaaa gcattcggtc catttcttgg
     3481 tggtgtatta gttgatgcgt ttaatatgcg catgatgttt atcggtatga tgctactact
     3541 tgtatttgca ttaatattat taatggtttt caaggagaat aatacgcaac ctaaaaaaat
     3601 agat
//
LOCUS       DAHHRR010000172         3523 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.176, whole genome shotgun sequence.
ACCESSION   DAHHRR010000172 DAHHRR010000000
VERSION     DAHHRR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.176"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            12..392
                     /locus_tag="NBI42_002496"
     CDS             12..392
                     /locus_tag="NBI42_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1778881.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            389..1285
                     /gene="pmtA"
                     /locus_tag="NBI42_002497"
     CDS             389..1285
                     /gene="pmtA"
                     /locus_tag="NBI42_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HCD1778882.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYAENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIFEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            1286..1966
                     /gene="pmtB"
                     /locus_tag="NBI42_002498"
     CDS             1286..1966
                     /gene="pmtB"
                     /locus_tag="NBI42_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HCD1778883.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            1963..2835
                     /gene="pmtC"
                     /locus_tag="NBI42_002499"
     CDS             1963..2835
                     /gene="pmtC"
                     /locus_tag="NBI42_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HCD1778884.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            2835..>3523
                     /gene="pmtD"
                     /locus_tag="NBI42_002500"
     CDS             2835..>3523
                     /gene="pmtD"
                     /locus_tag="NBI42_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCD1778885.1"
                     /translation="MRILNLVKYDLYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGDKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGS"
BASE COUNT         1251 a          445 c          587 g         1240 t
ORIGIN      
        1 gggggacgtt gatgaaaata attttaaaaa acaatagtga ttttccgatt tatgaacaga
       61 ttaagcaaca agtaaaacaa aatattttaa agggacatgt tgctcctgga gagcatttgc
      121 catcaatgag ggaacttgcc aaagatcttc aagtaagttt gattactacc aaacgtgctt
      181 atgaagattt agagaaagat ggttttgtta caacaattag aggaaaaggg acctttgtta
      241 aggaacaaga tagttctatt ttaaaagaga aacaattttt taccattgaa aatttggtta
      301 aagaattggt taatgaagcg caagccatcg aaatgtcact tgaggaactt caagatattt
      361 taacgttcat ttatgaggag gaatcatcat gaatgccata gaattaagta atgttaatta
      421 ttcaagtgat cagtttaatt taaagaatat ctctttcaaa gttcctcaag gattcgtcac
      481 tggttttatt ggtagaaatg gcgctggtaa aaccacaata ataaggttaa ttatggattt
      541 gtatcaacca caaactggtg ttattagagt attagaagag gatatggctc tcaatccgat
      601 agagttaaag aatagaattg gatttgttta cgctgaaaat tattttaacg agagatggac
      661 tacgaagcaa cttgaaaaaa tgattgcccc tttttatcgt aaatgggacc atcaagtatt
      721 tgaattttat cttgaaaagt tcgatttacc aatcaataaa tcaataaaaa cattttcgac
      781 tggcatgaaa atgaaattat cactagcagt tgcattttca catcatgctg aattatatat
      841 ttttgatgag cctacttcag gtcttgatcc cttagctaga aatgagctat tggagataat
      901 tcagcaagaa ttaattgatg aaaataaaac aatattcatg tcgacacaca ttatctcaga
      961 tttagaaaaa atagctgatt atatcatcca cttaagtgac ggtgaagtta tcttaaatgg
     1021 ttcaaaagaa caactgttac aaagatatca agttgtgagt ggtgcaattg aggaccttga
     1081 tgatgaatta gcatctctac ttatttttga agaacataag cgtacgggat ttataggatt
     1141 aacagaacat gcgcaagtat ttaaagaaat tttaggtcat aaagtgaata tcactacacc
     1201 ttctattgag aacctaatgg tttatcttga aaagagaaaa cctaaatatc atgaaaacat
     1261 caaattgatg gaagaaggtt tttaaatgaa ggcattgtta attaggaatt ttaaattacg
     1321 acgatataca cttatcatct atgttctatt attaacctta tatccttttt atataatgct
     1381 tgactcaaca aagttctttt acttattgca atcttttata agtccaacaa tattgattat
     1441 ttggatttta gatgctggtc acttatttag actcaatcgt agattaggtg gtaatgattc
     1501 atattatttt tatatgagtt taccagtgtc aaaaaaacaa ttgcttaatg ccaactatat
     1561 tacatgcatt gttttaacat taattggaac gcttgttatt agtttatatg cttatgaagc
     1621 agatgtgatt gaaccaaatt caatctattt ttcaactgca tatgcatttg tcatatctaa
     1681 cttcttgtct ataccaattg catttagtca atttacagaa ttgcgtagag tcaaagtgcc
     1741 atatggtata tacgtgttta ctattatcat tttagttcca tttttatttt caattgcaat
     1801 agtgttggtg aattattttg ttctaagtca atcatcattc ccagatctat attcatatat
     1861 tttaaatatt ggttttctaa ttataagcat tgttatactt attgttaatt attttaaaca
     1921 gctcaataaa ataaatacta gaaaatttaa aggaggcagt cgatgaaatt agaacatatt
     1981 acaaaaaaat acggctcaaa tgtcgtttta aatgatattg attttgactt tggcgatagt
     2041 agaattgtcg gattaatagg aaaaaacggt gttggtaaaa caaccgttat gaaagtaatg
     2101 aatggtaata ttattaaatt tgatggaaaa gtagatattg ataatgcaga taatatcggt
     2161 tttttaattg agcatcctaa attatatgat aataaatcag gattgtataa cttgaaatta
     2221 tttgcacaag tattaggtaa gggttttgat aaagcataca cagacaaaat tatagatgca
     2281 tttggtatga gaccttatat taaaaagaaa gttaagaaat attcaatggg gatgaagcaa
     2341 aagttagcaa ttgcagtatc tttaatgaat aaacctaaat ttttaatatt ggatgagcct
     2401 acaaatggta tggatccaga tggctcaatt gatgtgctga ctacaattaa gtctctagta
     2461 aatgaacttg atatgagaat tctaatatca agtcataagt tagaagatat tgaattaatt
     2521 tgtgatagag ctgtattttt aagagacgga cattttgttc aagatgtaaa catggaggaa
     2581 ggtgttgcat ctgacacaac gatagttact gttgatcata aagactttga tagaactgaa
     2641 aaatatcttg cagagcattt ccaattacaa aatgtcgaca aagcagacgg acatttaatg
     2701 atcaatgcac aaaaaaatta tcaagttata ctaaaagcat tatctgaatt agatatttat
     2761 ccgaaatata ttgaaacacg taaaagttca ttgcgtgata cgtacttcaa tataaatcaa
     2821 agaggtgata aataatgaga attttaaatt tagttaagta tgatctttat agtattttta
     2881 aaagtccttt aacatattta gcgatactag tcgtatctag tttgattgca actcaaagta
     2941 tacttatggc aaattcgatg gataacccga aacatattat tgtctatgga tctgtatttg
     3001 ctgcagcaaa atggttattg ttaataattg gattaatgtt tgttgttaag acaattacgc
     3061 gtgatttttc acaaggtaca attcaactat atatgagtaa agttaaaaca cgcgttggat
     3121 acattatttc gaaaacaatt tcaattattt taatttcaat attatttgca ttaattcatt
     3181 atgtgatttt gattgttgtg caggcatcta gcaatggaaa aaatttggcg ttttctaaat
     3241 atgtagataa tttatggttc ttcctaatct ttttactatt ctttggcttg tttttattct
     3301 taatcacact tgcatcacaa aaaacagcaa tgatattttc attaggtgta tttttagtac
     3361 tcattgtacc gtttattaaa ccttttatta catttatccc aagatacggt gataaagttt
     3421 tagatgcttt tgattatatc ccttttgctt acttaactga taaaatgatt agctctaact
     3481 ttgattttag caattggcaa tgggtaattt cattaggttc tat
//
LOCUS       DAHHRR010000173         3493 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.177, whole genome shotgun sequence.
ACCESSION   DAHHRR010000173 DAHHRR010000000
VERSION     DAHHRR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.177"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            179..1561
                     /locus_tag="NBI42_002501"
     CDS             179..1561
                     /locus_tag="NBI42_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCD1778886.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGFKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDREMAFSSSKTYGPYPQNKDITISASGKVKGKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            1592..1720
                     /locus_tag="NBI42_002502"
     CDS             1592..1720
                     /locus_tag="NBI42_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778887.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCEKIYGVLRFISFYTFYKL"
     gene            1830..3038
                     /locus_tag="NBI42_002503"
     CDS             1830..3038
                     /locus_tag="NBI42_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCD1778888.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVIAPTLGGII
                     LNYSIWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRVMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGIASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(3265..>3493)
                     /locus_tag="NBI42_002504"
     CDS             complement(3265..>3493)
                     /locus_tag="NBI42_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HCD1778889.1"
                     /translation="PMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAG
                     VFINWLSNGCNESPEEVADILLANTIKLQNQ"
BASE COUNT         1255 a          445 c          625 g         1168 t
ORIGIN      
        1 tataattgag agagtacttt cataaataat gtaataaggt acaattctaa agtaaagtta
       61 atcatttctt gaattgtatt cttgggataa tatttgtaaa ataataatgg taaaataata
      121 aaataatttt tgtgatatac atataacaat tgaagaggaa cagaattgag gtgaaagtat
      181 gaaatcttgc ccgaaatgcg gacaacaagc acaagatgat gtacagatat gtacacaatg
      241 tggacataaa tttgatagtc gtcaagcatt atatagaaag tcaaccgatg aagatataca
      301 aactaataac atcaagatga gaaaaatggt gccttgggca attggttttt tcattttaat
      361 cttaattatt atattgttct ttttactaag aaacttcaat tcacctgaag cgcaaactaa
      421 aatattagtg aatgcgattg agaataatga taaacaaaaa gtagcaacat tattaagtac
      481 taaagataat aaagtagatt ctgaagaagc gaaagtatac attaactata tcaaagatga
      541 agttgggttt aagcaatttg tcagcgacct taaaaatacg gtacataaat tgaacaagag
      601 taagaccagc gtagcttctt atattcaaac cagatctggt caaaatatat tacgtgtaag
      661 taaaaatggc acgcgttata tctttttcga taatatgagc tttactgcac ctaccaagca
      721 accaattgtt aaaccgaaag aaaaaacaaa atatgagttt aaatctggtg gtaagaaaaa
      781 gatggttata gctgaagcaa ataaagtgac gccaataggt aattttatac cggggacata
      841 tagaattcca gctatgaaat caactgagaa cggtgatttt gcaggccatt taaaatttga
      901 ttttagacaa agtaattctg aaacggtaga tgttactgaa gattttgaag aagcaaatat
      961 atctgtaact ttaaaaggcg atacaaaatt aaatgatagt tctaaaaaag taactataaa
     1021 tgaccgtgaa atggcatttt caagttccaa aacgtatggt ccatatccac aaaataaaga
     1081 tattaccatt tcagcttcag gtaaagtgaa aggtaaaaca tttacaacac agacgaagac
     1141 gattaaagcc agcgatttaa aatacaatac agagataact ttgaattttg acagtgaaga
     1201 tatcgaagac tatgttgaaa agaaagaaaa agaagaaaac agcttgaaga acaaattgat
     1261 agaattcttt gctggatatt ctttagcgaa taatgctgcg tttaatcagt cggattttga
     1321 ttttgtatcg tcatatataa aaaaaggatc atctttttat gatgatgtaa agaagcgtgt
     1381 atctaaagga agtttaatga tgattagttc accacaaatt atagatgccg aaaaacatgg
     1441 tgataagatt accgcaaccg tacgattaat aaatgaaaac ggtaagcaag tagataaaga
     1501 atatgagctt gaacaaggtt cgcaagaccg cttgcaatta atcaagacat cagaaaaata
     1561 gagtgtaaaa cttaaataag atgcatgtgt catggaatac aaacaacatc ctttcaaagt
     1621 atttacaatt agtgtgaatt actttgaagg gattgttttt tgtgaaaaaa tttatggagt
     1681 gctgaggttt atatcatttt atacctttta taagttgtaa tgatgttcat gaatcttgtg
     1741 agtaatgcta atatataaaa gagcatattt aaaattagtt tctgttcgtg atagttataa
     1801 attaatcatt atataaagga gatagtcata tgaatgattt aacgcatttt aacaagcgtt
     1861 caccgatatt tattattatt ttaggatctt tgacggccat tggagcatta tcaatagata
     1921 tgtttttgcc tggattacca gatattagac atgattttca aacaacgacg tctaacgcac
     1981 aattaacatt atcgatgttt atgattggtt tagcgtttgg taatttgttt gctggtccaa
     2041 tttcagattc aactgggcgc agaaagccgc ttattatagc tatgattatt tttacattag
     2101 caagtttagg tattgttttt gtacataata tttggctgat ggttgcatta agatttcttc
     2161 aaggagtgac aggtggagca gcagcagtta tttcaagagc tatagcaagt gacatgtata
     2221 gtggtaacga attgacgaaa tttatggcat tattaatgct tgtaaatgga atagcacctg
     2281 tcattgctcc aactttaggt ggtattattt taaattattc tatttggaga atggtgttcg
     2341 taattttaac tatttttggt ttcgtcatgg tcataggttc tttattaaaa gtacctgaat
     2401 cattaacagt gacaaatcgt gagtcaagta gtggattaaa gacaatgttt aaaaacttca
     2461 aaatattatt gaagacgcca cgttttgtat taccaatgct cattcaaggt atgacatttg
     2521 taatattatt tacttatatt tctgcgtctc cgtttattat tcaaaaaatt tatggcatga
     2581 ctgcaataca atttagttgg atgtttgcag gaattggtat tacgctcatt atatcaagtc
     2641 aacttactgg gtaccttgtt gattttatag actcacaaaa attgatgaga gtcatgacga
     2701 tgattcagat tatcggtgta attttagtga cgattgtatt gcttaatcat tggaactttt
     2761 ggattttagc gataggattt atcattttaa tagcaccagt gacaggtgtt gcgacacttg
     2821 gttttacaat agctatggat gaaagtagta gtggtagagg gagttcatca agtttattag
     2881 gtttagtgca gtttttattt ggtggtattg catcgccatt agtgggggta aaaggtgaag
     2941 ataatcctat accttacata attattatta tagctacagc agttatatta attattttac
     3001 agatttataa tatgaaggta tttaaaacaa atcgatgata aacatagttt cttctattat
     3061 attgaattga aaaattaaaa aacaaaacat gagctattca tagacctatg aatttagcat
     3121 caaaaataaa aatgccgaga ttattatgtg gaaatctcgg catttttata ttgatagcac
     3181 cgttacatat gtactattat ctgtccattt ggtttaactt tatctagttg ttttatagca
     3241 tttctttatt agaatgaaac catattattg gttttgaagt ttaatcgtat tagccaataa
     3301 aatgtcagca acttcttcag gtgattcatt acaaccattc gaaagccaat taataaacac
     3361 cccagcttga ccgcctatga tatatgtaac ataaagctgt ttattggtta ctgtagtact
     3421 atagtcattc ataatttgtt gatagtaatc tctcgctaaa tttatatagt ctatgaataa
     3481 tgttttcatt gga
//
LOCUS       DAHHRR010000174         3470 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.178, whole genome shotgun sequence.
ACCESSION   DAHHRR010000174 DAHHRR010000000
VERSION     DAHHRR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.178"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..979
                     /locus_tag="NBI42_002505"
     CDS             <1..979
                     /locus_tag="NBI42_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD1778890.1"
                     /translation="VDIKKHVKDKAKVNYQVPYSINLNGTSTNILSNLSFSNKPWTNY
                     KNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKD
                     VKRIEITVKTGPKAKADRYVPYTIAVNGTSTPILSKLKLSQKELISYKYLNDKVKSVL
                     KSERGISDLDLKFAKQAKYTVHFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQH
                     TKSKKINKSNNVKFPVTINKFENLVSNEFVFYNARKITINDLSIKLKSAMANDQGITK
                     HDIGLAERAVYKVYFKNGSSKYVDLKTEYKDGKVFKATDIKKVDIELKF"
     gene            complement(1258..2544)
                     /locus_tag="NBI42_002506"
     CDS             complement(1258..2544)
                     /locus_tag="NBI42_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1778891.1"
                     /translation="MNPLAQSLNEQLQQSNATAFAMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMLLNYPNNPTGYTPTHDEVTTIVNAIKT
                     LADKGTKIVAVVDDAYYGLFYEDVYTQSLFTALTNLNSNAVLPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            2744..2842
                     /locus_tag="NBI42_002507"
     CDS             2744..2842
                     /locus_tag="NBI42_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778892.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            3085..3261
                     /locus_tag="NBI42_002508"
     CDS             3085..3261
                     /locus_tag="NBI42_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778893.1"
                     /translation="MKHLTKVFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
BASE COUNT         1250 a          475 c          569 g         1176 t
ORIGIN      
        1 tgtagatatt aaaaaacatg tgaaagataa agctaaagta aattatcaag ttccatattc
       61 aattaattta aatggtacat ctacaaacat tttatcgaat ctttcatttt caaataaacc
      121 ttggacaaat tacaaaaatt taactagtca aataaaatca gtactgaagc atgatagagg
      181 tattagtgaa caagatttaa aatatgctaa gaaagcttat tatactgttt attttaaaaa
      241 tggtggtaaa agaatcttac agttaaattc aaaaaattac acagcaaact tagttcatgc
      301 gaaagatgtt aagagaattg aaattactgt taaaacagga cctaaagcga aagcagacag
      361 atatgtacca tatacaattg cagtaaatgg cacatcaaca ccaattttat ctaaattaaa
      421 actgtctcaa aaggaattaa ttagttacaa atatttaaat gacaaagtga aatctgtatt
      481 aaaaagtgaa agaggcatca gtgatcttga cttaaaattt gcgaaacaag caaaatatac
      541 agtacatttc aaaaatggaa agaaacaagt agtgaattta aaatcagaca tctttacacc
      601 taatttattt agtgccaaag atattaaaaa gattgatatt gatgtaaaac aacacactaa
      661 atcaaaaaaa ataaataaat ctaataatgt gaaattccca gtaacaataa ataaatttga
      721 aaacttagtt tcaaatgaat ttgtgttcta taatgcacgc aaaattacaa ttaatgactt
      781 aagtataaaa cttaaatcag caatggcaaa tgatcaaggg ataactaaac atgacatagg
      841 actggctgaa cgcgcagtgt ataaagtgta ttttaaaaat ggttcgtcaa aatatgtaga
      901 cttaaaaact gagtataaag atggaaaagt atttaaagca actgacatta aaaaggtaga
      961 tattgaactt aaattctaat ctttaatttt ataataaggc atctcacaat cgtggggtgc
     1021 cttttacatt tgtagagatg tgatacttga atgcttgaag tgatttgcca aaaattatat
     1081 aaatttgtat caaacaaatt aaattttgtt aggagagatg tataaaacta attaggtata
     1141 gcgtttgaca agttgctgaa taaaatatat cttatgatac tttgaaataa ggaataattt
     1201 taaaaataaa aaaccaataa tccgagtcat actcatcaga ttattggttg gaattaatta
     1261 tcttaagtca tcaatcgctt tagcaattga atcaaataca tgtgggatat cttctttttc
     1321 tacacagcta aacgcaatac gaatatctgt tgcatttagt gcaataacac caattgaata
     1381 tttttcaatt aaatgtttac gaagttcttc agggtcaaca tcatgaactt ttattgccat
     1441 aaagtatcca gagttaaagt cataggcttg ccaatgtgaa tgatattgat cagcatagac
     1501 aacttcctta gtgacttcat agcgttcttt taatgtttga atattttgtt cgatttcttt
     1561 atcgaattgt ttattatttt ttaatacatg cttaacagcg ctttgtgttg gaagaggtcc
     1621 actagaaatg ttacttcgta taagaccttt tactttggct tctaatactt ctttagttgt
     1681 ttgatcagac gttccaaatg tcataaaccc aacacggaat ccccatgcga aaaattcttt
     1741 tgtagcgcca tctaaacgaa ccggtagtac tgcatttgaa tttaagtttg ttaaagctgt
     1801 aaataatgat tgtgtatata catcttcata aaaaagacca taatatgcat catccacaac
     1861 agctacaatt tttgtgcctt tgtcggctaa cgttttaatc gcattgacaa ttgtagttac
     1921 ttcatcatga gtaggtgtat agccagttgg attgtttgga taatttaaaa gcataataac
     1981 tttatcttta ttgtatgatt gtaaagcttc tactagtgaa tcagtagtat aatgcccgtc
     2041 tttatcaaag ataggatatg tttgaaggtt tgcaccattt ctcgtattga aaacaagttt
     2101 gtaattaccc caattatgct ctggtaacaa gatagtgtca ccttgattta caaataaatc
     2161 gccaactaaa gataaaccat gtgttaatgc attcgtaaca attggtagag acatgttgtc
     2221 gattgatagc tctggattgt cacgcaacat tttttgttgc cataaatcac gtaattcctc
     2281 gatgccttgt ggtggcgcat aagggaatat ttcatctgga gttaaatcat taaacattgc
     2341 atctaaagat gatgcaaaca tttttccgtc tttgtttgtc gccataccta tagttgcatt
     2401 atatgttgta ctctttgctt cagcagattg agataaaatg ccttttggat aaaacatatt
     2461 ttgacctaag tcagataaca ttgcaaaggc agttgcattt gactgttgaa gttgttcatt
     2521 taagctttgg gctaaaggat tcattattgc ttaacctctc tttaaaaaaa ttgtatgcct
     2581 atatattatc ttcaattata ccaaagataa agtgttgttg gtgaattaac gaataatatg
     2641 taaaatccgc taccaaaatg cgaacatatg ttctgatttt gtgttagaat aggtgtaggt
     2701 catccttcag tatgatactt tagtcgaacg gaggtgtagt gtaatgatgt ggtggcaaga
     2761 agggatgatg acacttatta caggtggctt actcattatt tttcgacttt ggttagaact
     2821 gaaatggaaa aataaaaaat gatcctacac acataaataa ccccaagtct gctggtacca
     2881 gacttggggt tgtgtagtat aattctgtgg atacacttaa ataatatcat tgaacacaat
     2941 atatcgcaat caataatcat tttgattgaa tggttttgtt gcgctatttt agttaaaagt
     3001 gattagcccc ttgaactgtt gaaattcaat aacttattaa aaaaatttat atttgttata
     3061 atatcgatgt tgaggtgaaa gaagttgaaa catctaacga aagtatttgt agtacttgca
     3121 attattttat ttattattgg ttattattta caagctacta atcatgaaag ccaaggtata
     3181 aaattattat tagcagcgat tatgtttatg atttgtgctt ttataagtag aagtaatgat
     3241 cgacgtaaaa atagtaaata gaattgactg tttaaaacga ttgtttatgt tgacgctacg
     3301 gttgtcacgt agagattaaa gacattcgtt ttagcagtct tttttattga agtagacaat
     3361 gcaagattaa atggtagtgt catttcattt tattcaaatg ctagtaatac aatactcagt
     3421 gttatttctg aagactggtt gataatgaga catgtatatt tacaaaactg
//
LOCUS       DAHHRR010000175         3451 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.179, whole genome shotgun sequence.
ACCESSION   DAHHRR010000175 DAHHRR010000000
VERSION     DAHHRR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.179"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..557
                     /locus_tag="NBI42_002509"
     CDS             <1..557
                     /locus_tag="NBI42_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD1778894.1"
                     /translation="IKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNRKKILFR
                     EVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASLSKKARY
                     NVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHHLEHLDR
                     IRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(647..739)
                     /locus_tag="NBI42_002510"
     CDS             complement(647..739)
                     /locus_tag="NBI42_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778895.1"
                     /translation="MFDFSTILKRKVVTGWWGHNIEKAVRDFLN"
     gene            complement(748..2484)
                     /locus_tag="NBI42_002511"
     CDS             complement(748..2484)
                     /locus_tag="NBI42_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HCD1778896.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     ITQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(2775..3317)
                     /locus_tag="NBI42_002512"
     CDS             complement(2775..3317)
                     /locus_tag="NBI42_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCD1778897.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
BASE COUNT         1171 a          624 c          461 g         1195 t
ORIGIN      
        1 atattaaaca agatagtcat aatttaaaac aacgtgtcga aaagttagag aaaatttatg
       61 attactatag cgaagaacgt cctattacaa aaaaacatat tcaccaacag aatagaaaga
      121 aaatactttt cagagaagta gttcagacga ctagacaagc ttatgaagta ttaaacaaat
      181 tgtcgcgtta tcaaaacgac ttatatttac ttaataacaa tttcctatta caaatcaaat
      241 tagatttaga ttcattaact gcttttcacg agcaaatttt agctagcctt tctaaaaaag
      301 cacgttataa tgtgacacat gttgattatg agttagataa tccgcagaaa aaagatttat
      361 tgtctacatt tcagcatgag ttaataaatc atccttatca aacggaatat tcgtttgcca
      421 atgttatgca aattgttgca gccatcgaag agtatcgaca ccatttagag catttagacc
      481 gcataagaat tagctttttc acatatcata gatctgatgc agacattgaa attgttgaag
      541 aagactttga tttataacat taaaacaatt cctgtaatta gtaccacaat gagataaagt
      601 aatattgaat ctcgaaatta tatttaaaaa agcagtaaga ttattttcaa ttaagaaaat
      661 ctcttactgc tttttctatg ttatgacccc accatcctgt tacaactttt ctcttcaaga
      721 ttgtactgaa gtcaaacaac taatacttta taagttttga atgctatata aatgctcata
      781 agcaccttgt tttgcaatca attcacgatg cgtacctgtt tcaacaatat gcccattttc
      841 aattacgaca attttgtcag catgtgtaat agtggacaag cgatgcgcta cgataagtgt
      901 cgttcgatct ttactcaaca catctaatgc ttcttgaata atggattcac tttctaaatc
      961 aagtgcactt gttgcttcat ccaagataag aattggtgga ttatttaaaa atattctagc
     1021 aatcgataat ctttgttttt gaccacctga tagtttaaca cctcgttcac ctacttcagt
     1081 gtcatatccc tgtggcaagt tcataataaa gtcatgtgca ttagccattt tcgccgcttc
     1141 aactacttct tcatctgttg ctgttggacg accaagtaaa atattttcct taactgtatc
     1201 agagaataaa atattatcct gttgcaccaa tcctatttga tttcttaaac ttcccgttaa
     1261 aaaatcttta atgttgtgac catctattaa aatttgccca gaagttacat cgtaaaatct
     1321 cggtattaag ttaattaatg ttgatttacc accaccactc atacctacga aagcaactgt
     1381 ttctcctttt tcaatactca aattaatatc ttttaaaatt ggagcttcgt tatcgttata
     1441 ttgaaaacta acatgatcaa tatcaatacg accttgtgta atttcaatag gttgagcacc
     1501 aacaccattt ttaatgtcat aatcttcgtc aattaattgg aatacacggt ccattgaagc
     1561 aaaactttgc gttaaagttg taaatgatgc gactaaacga cgtaaaggcc cgaacaataa
     1621 ctccaagtat ccaacaaatg ctgcaagtgt acctactgtg attgatccag aaatagcaag
     1681 atatgcacca acaccgatga caattattgg tccaatatct gtaactgtat taattgcggc
     1741 aaaggaatag gcattccatc ttgtatgttt caacgcacgt gttaagaaat tagtgttctt
     1801 tttatcaaag tttttcgctt cattgtcttc aatcgcaaaa cttttaacga ctgaaatacc
     1861 ttgaacacgt tcatgcaaga atccttgaac ctcagctaat gcttgggatc tttcacgcgt
     1921 caattttctt aatcttccaa agaaaacgta caccgttaaa atgtaaaatg ggaagataaa
     1981 cagtgctgct aaagtcaatt tcacatctaa aaagaacatt atggatagtg caataataat
     2041 tgttatacaa tctaaccaaa tattcattaa cccggttaaa atgaaatctt ttgtttgttc
     2101 aacatcatta atcactctag atattacttg acctacttga ttattagcat aaaatctcgc
     2161 acttaaagct tgtaaatggt tgtataactt tttacgtata tcatacaata ttttattact
     2221 tgtccattgc gccaaatatt gacgtataaa ttcaattggt ggtctcacta ttacaaaaat
     2281 aaataatgcg ataccaattg caatagttaa atgatgaact ttttcatcag tcgttagtgc
     2341 gtggttatta atcacgccat ctattgcata tttaattaaa agtggtataa gcattggtat
     2401 accaaactta attatcccaa caataatcgt tgcaaaaata cgatatttgt atggcttaac
     2461 aaattgcaaa tatcgtttaa tcatacaatc ccccctaatc tattgcccta tcctattcat
     2521 aagcataaaa atgaatagag gttggataca taatttgtag atgtaaatcc ttcttacaat
     2581 ttacattttt aaaattaaga tatctcaacc tctgtcatat tcatgtgatt aaatacgttg
     2641 tcttattcgt atgttatatt ctattcaatt taatctatgg atactgtgtc cccacacgac
     2701 agcaaaagtt atcatacttc tttacatcac taagtcaata taaatgattt aatcagtatt
     2761 tacactttat ttgcttaata ttgtctaatt tttttgtaac gttctttcca aactttgata
     2821 aaatctggcg cgaatgggcc tttcttctgt tctatccatt gttgaagaat gtccacgttg
     2881 cgtcttaaaa taatatcaat atcatgcgga taattcattt gattcatatg ttgctcatat
     2941 tcatcttcat ctaataaatg atactttccg tttggatata ctttaatatc taaatcatag
     3001 tctatatatt ttaatgcctc ttcatcacaa acaaatggtg atgacaaatt gcaatagtaa
     3061 taaattccat cttctctaaa catgcagata acattaaacc aatattctga gtgaaagtaa
     3121 acaattgccg gttcacgtgt tatccaagtt cttccgtcac tttcagttac taacgtatga
     3181 tcattgccac caatgacaac atgatcagta ccctttaata ttgttgtttc agaccaaacg
     3241 cgatgaatct taccatcatg tttataactc tgaattttaa tgttttcccc ttctttaggt
     3301 atggattctc tgaccatact ccacaccacc ttctgttaat ttaaccatta taaattatag
     3361 catatttcag aaatagtatt atataaatac atatttttac gaaataagat tttactactt
     3421 aatgattaaa cttggtaata ttgctaagta c
//
LOCUS       DAHHRR010000176         3439 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.180, whole genome shotgun sequence.
ACCESSION   DAHHRR010000176 DAHHRR010000000
VERSION     DAHHRR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.180"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            4..120
                     /locus_tag="NBI42_002513"
     CDS             4..120
                     /locus_tag="NBI42_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778898.1"
                     /translation="MLFNKVLSRILTYQLNFLNSHQKDKTLHSTNLTRKNKK"
     gene            281..1267
                     /locus_tag="NBI42_002514"
     CDS             281..1267
                     /locus_tag="NBI42_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HCD1778899.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIHMEFNA
                     NVSQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGIAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(1382..2350)
                     /locus_tag="NBI42_002515"
     CDS             complement(1382..2350)
                     /locus_tag="NBI42_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCD1778900.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNEKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNMPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            2427..3086
                     /locus_tag="NBI42_002516"
     CDS             2427..3086
                     /locus_tag="NBI42_002516"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCD1778901.1"
                     /translation="MNAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            3129..3233
                     /locus_tag="NBI42_002517"
     CDS             3129..3233
                     /locus_tag="NBI42_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001803823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778902.1"
                     /translation="MHIQIECADNDLLKLKGDFYSMMNESCLFIFGKS"
BASE COUNT         1248 a          510 c          611 g         1070 t
ORIGIN      
        1 agtttgttat ttaacaaggt gttgtctcgg atattgacat atcaattaaa ctttttaaat
       61 agtcatcaaa aagataaaac actacattca acaaatttaa cgaggaagaa taaaaaataa
      121 atcaacatat taaattgtag tgttattcaa ctccgtagct aacaattctc tatgcacatt
      181 aaacaaattg tcaaaaatat atcataaatc ttcaagcaca gtcttagcgc atcaatcact
      241 gaactgttat aatagtttgg gattaaagga ggccgaaaca atgcaaaaag ttgaaagtat
      301 cataattggt ggagggccat gcggattaag tgcggctatt gaacaaaaaa gaaaaggtat
      361 tgatacctta attattgaaa agggtaatgt cgttgaatca atctacaatt atcctactca
      421 ccaaacattt ttctcatcaa gtgataaatt aagtattggg gacgtaccgt ttatcgttga
      481 agaaagtaaa ccaagacgaa atcaagcgct agtttattac cgagaagttg taaaacatca
      541 tcaattaaaa gtaaatgcat ttgaagaagt attaactgtt aaaaaaatga ataataaatt
      601 tactattact acgacgaaag atgtttatga atgtcgattt ttaacaatcg cgacaggcta
      661 ttatggtcag cataatacat tagaagttga aggtgcggat ttacctaaag tgttccatta
      721 ttttaaagag gcacatccgt attttgatca agatgttgtt attattggtg gtaagaattc
      781 ggctatcgat gctgctttgg agttggaaaa agctggtgct aacgtgacgg ttctatatcg
      841 tggtggagat tattcgcctt caattaaacc ttggatactt ccaaatttca cagcattagt
      901 aaatcatgaa aaaattcaca tggaatttaa tgctaatgtt agccaaataa ctgaagatac
      961 tgtgacttat gaagtaaatg gtgaaagtaa aacgatacac aatgattatg tatttgcgat
     1021 gattggttat catcccgatt atgaattttt aaaatctgta ggcattcaaa ttaatacaaa
     1081 tgaatttgga atagcgccta tgtataataa agaaacatac gaaacaaata tcgagaattg
     1141 ctacattgca ggtgtgattg ctgcagggaa cgatgcgaat accattttta ttgaaaatgg
     1201 taagttccac ggtggcatta ttgctcaaag catgctagct aagaaacaaa cgccgttaga
     1261 atcataaaaa taaaggtcta cgtactaaat aacttagttt tacaacgact gacattcatg
     1321 atatgtcaaa tgaggttgat gactaattat tgtacataga cctttttatg ttacgtaatc
     1381 gttataattc aaaatatgat ttgatttcag ctttatctaa attgttgctt aacgcgacta
     1441 ataattttaa tcttgctttt ggaccattca aaccattaga aaaaataaaa ccttgttgtg
     1501 cgagttggta accaccacca tcgtatgcgt aagttggact cacaatacca ttaaaggaac
     1561 gtgaaactag cacaataggt atatttaaag atactaattg ttgaatgcct tctaatgcgc
     1621 ttggaggcat gttgccttgt cctaacgctt caataaccat accatcgata ccttcacgac
     1681 tataaaaact aaaaatgtca cctggcatac ccatatatgc ttttactaat ggtacattta
     1741 gtttctcatt gacattttct aatgcttgtt ggcgatatgg catatgatgg aattgcacac
     1801 gatcctttgt caatacacct agcggaccat gatttggact ttgaaatgtg tttgtattag
     1861 atgtatgtgt tttggtaaca ttacgggccg tatgtatttc atcattaaat acaaccatca
     1921 cacctttatg acgggccttt tcatcagagg caacgcgaat agcggaaata taattatata
     1981 atccgtcaga accaatttca ttagacgagc gcattgcgcc agtaataaca acaggttgct
     2041 cgatacctaa tattaaatca agtaaaaagg cagtttcttc taacgtatcg gtaccatgtg
     2101 tgataacaaa accatcataa tatttatttg ttactgcttc caaaataata tcctttaatt
     2161 gtttaacatg ttggattgtc atatgaggtg atggtacatt aaaaggattt aattcatcta
     2221 tttgtgcata ttgatttatg acatcttgat gcattgaaat agggttgata tcatttgtta
     2281 ctactttatt agattggtct tgtgacatac taatggtgcc accagtatga ataacaagta
     2341 gatgtttcat atatttcctc ctatatttca tttacctaat tatgataaaa tattattcat
     2401 aaaacgacaa ggaagggaaa tgacgcatga atgccattaa tattgcatta gatggtccag
     2461 ctgctgccgg aaaaagtaca attgcgaaac gtgtagccag cgaactatca atgatttatg
     2521 tcgatacagg agcaatgtat cgtgcattaa catacaaata tttaaaatta aacaaaactg
     2581 aggactttgc aaaactagtt gaccaaacaa cattagattt aacttataaa gcagataaag
     2641 gtcaatgtgt cattttagat aacgaagatg taacagactt tttaagaaat aatgatgtga
     2701 cgcaacatgt ttcatacgtt gcatctaaag agccagtacg ttcattcgcc gttaaaaaac
     2761 aaaaagagtt agctgcagaa aaaggtatcg taatggatgg tcgcgatatc ggaactgtag
     2821 tgctaccaga tgcagattta aaagtatata tgattgcatc agttgaagag cgagcagaaa
     2881 gaagatataa agataatcaa ttaagaggta tcgaatcaaa ttttgaagat ttaaaacgtg
     2941 atattgaagc acgtgatcaa tatgacatga accgtgaaat atcaccatta agaaaagcag
     3001 atgatgcagt gacattagat acgacaggca agtcgattga agaagttact gacgaaattt
     3061 tagcgatggt gagtcaaatt aaataatcta gtaagtcata actatttatt tgtaatgggc
     3121 aacgcttaat gcatatacaa attgaatgtg ctgataatga tttactcaaa ttaaaaggtg
     3181 atttttattc aatgatgaat gaaagttgcc tttttatttt tggtaaaagt taatgcggca
     3241 gtcaattgtg taagtttttc aaaaagtaga cagaaataat aaagataaat tattagaatt
     3301 ccaaaaataa ttcattacat tcataaagca ttttacaaat gttaagaaaa gagtgttaca
     3361 aatctaagta ttgcaaaaga agctgattta gtcacaaaaa atgtcctatg taataattcg
     3421 agaaagatgc actatatac
//
LOCUS       DAHHRR010000177         3254 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.181, whole genome shotgun sequence.
ACCESSION   DAHHRR010000177 DAHHRR010000000
VERSION     DAHHRR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.181"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..845
                     /locus_tag="NBI42_002518"
     CDS             <1..845
                     /locus_tag="NBI42_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1778903.1"
                     /translation="ISFEIEQGKCIALIGKNGAGKSTLIDILIGKVNANSGEIFDKDK
                     LLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENPLPLEEIIELTKFDSSQLNQFVN
                     KLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEIREYFWSIIENLKEDNRTILYTS
                     HYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQSQITLSEEYKRKLKLDKDDLVIQ
                     KNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKVSIVDSYFNNKKQRGSNYDTKLL
                     ENRI"
     gene            814..1545
                     /locus_tag="NBI42_002519"
     CDS             814..1545
                     /locus_tag="NBI42_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1778904.1"
                     /translation="MILSYLKIEFKVIMRKKTTLLLSILFPVIFYILFTSILDLPEDV
                     KPKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNGWRQRLMVTPFTFTSYYISKVVK
                     TMLQFALAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQK
                     TSTLANIITICLAVLGGLWFPIKTFPNWLQHVAHVLPSYHLCKLGVDIASNHHINLIS
                     FGIIILYALGSIIAVYCIRSFKKAE"
     gene            1549..2640
                     /locus_tag="NBI42_002520"
     CDS             1549..2640
                     /locus_tag="NBI42_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1778905.1"
                     /translation="MKFLKGNSIAELSSLLYLIFPIAGIFFNEIHGPKWLYIILVSIF
                     SVSYFILVTLNNRLNHLTLYILLMIHYVIICYFVLFIHPMLSLFFFYSAFAIPFTFKK
                     SVKKASLYMFTLTLIICSIITYSFYNDYFVAIMIYYVVILLIILDNFKKMRNREYQKE
                     IEEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDSDIEKTKAELL
                     AINKLSRESLNKVREIIDDIKLPSFIEEIDSIGKVLNDADIDFTFENKELAQVLSPTK
                     QSMLVMIAREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSGCEVKSISQ
                     RVQHLNGTLAVDSTNGTKIIIEISTGGIV"
     gene            2637..3239
                     /locus_tag="NBI42_002521"
     CDS             2637..3239
                     /locus_tag="NBI42_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1778906.1"
                     /translation="MISLIIAEDQNMLRQAMVQLIKLHGDFEILADTYNGLDAMKLIE
                     EYNPNVVILDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSTIIDKLFADNRFDAWKKANEKGWI"
BASE COUNT         1224 a          386 c          525 g         1119 t
ORIGIN      
        1 atatttcgtt cgaaattgaa caaggtaaat gtatcgcttt aattggaaaa aatggtgcgg
       61 gaaagtcaac gttaattgat atattaattg gtaaagttaa tgctaattct ggtgagatat
      121 ttgataaaga caagttatta caaagtgaaa atcgcagtat aatgttccaa aaaacgatgt
      181 tcccagatca attaaaagtt attgagatta tcaacttata tcaatcattt tacgaaaatc
      241 cattaccatt ggaagaaata atagaactga cgaaatttga ttctagtcaa ctgaatcaat
      301 ttgtaaataa actttctggt ggtcaacaac gattactcga ttttgtatta tctttaatcg
      361 gacaaccaca attgatctta ttagatgaac caacatcgac tatggatata gaaattagag
      421 aatatttttg gtcaattatt gaaaatttaa aagaagataa tcgaacgata ctttatacat
      481 cacactatat tgaagaagtc gaacgtatgt cagacaaaat tattctcatt gaaaatggag
      541 aaataatact taatgattca acgtcacaca ttagaaccaa tcagcaatca cagattacgt
      601 tatccgagga atataaaaga aagttaaaac tagataaaga tgatttagtt attcaaaaaa
      661 atcataatgg cactatcaaa attattactt cgaatgtaaa tgatacgatt ttatatcttc
      721 aacaacttca tattaatttg gatgatattg aaatacaaaa agtctcaatt gttgattcat
      781 acttcaacaa taaaaagcaa aggggatcta attatgatac taagttactt gaaaatcgaa
      841 tttaaagtta taatgcgtaa aaaaacaaca ttattattat ctattttatt tcctgttata
      901 ttctatatat tatttacttc gatattggat ttgccggaag atgttaaacc taaattttat
      961 aaagagtata tgtatagtat gaccgtttat agtttgttaa gttttagctt actaactttt
     1021 ccattagata ttattaatga aaaacaaaat ggatggcgcc aaagattaat ggtaacacca
     1081 tttactttta ctagttatta tatttcaaaa gtagtgaaaa ctatgctgca atttgcattg
     1141 gcgatattag ttatttttat ggttggacat ttttataaag gtgttgcaat gagtgcagtt
     1201 caatggttag agtcaggaat atttttatgg ttaggtgcgt ctctattaat aacttttggc
     1261 atattatttt ctttgttaaa tgatattcaa aaaacaagta ctttagctaa tatcataaca
     1321 atttgtttag cagtattagg tggattgtgg tttccgataa agacatttcc aaattggctt
     1381 caacatgttg ctcatgtttt accaagctat catttgtgta aactaggtgt agatattgct
     1441 tcaaaccatc atatcaattt aatatcattt ggtataataa ttttgtatgc tttaggaagt
     1501 ataatagcag tctattgtat tagaagtttt aaaaaggcgg aataaaaaat gaaattttta
     1561 aaaggtaatt caattgctga actatcctct ttactttatc tgatttttcc tattgctggt
     1621 atttttttca atgaaataca tgggccaaaa tggttgtata tcatactagt tagtattttt
     1681 tcagtatcgt attttatatt agttacacta aataatagac ttaatcattt aacactttac
     1741 atattgttaa tgatacatta tgttattatt tgctattttg tattatttat acatccgatg
     1801 ttaagtctgt ttttctttta cagtgccttt gctattccct ttacttttaa aaagagtgtt
     1861 aaaaaagctt cactgtacat gtttacacta acactgatta tatgttcaat aattacttat
     1921 tctttttata acgactattt tgttgcaata atgatttatt atgtcgttat attattaata
     1981 attctagata atttcaaaaa aatgagaaat cgtgaatatc aaaaagagat agaagaaaaa
     2041 aatcgacata ttaatacatt aattgctgaa caagagcgac atagaatagg gcaagactta
     2101 catgatacgt tagggcatgt gtttgcaagt ttatcattaa aatcagaatt agcttataaa
     2161 cttatagatt cggatataga aaaaacaaaa gctgaattat tagcaattaa taaattatct
     2221 cgtgaatcac taaacaaagt tcgagaaatt attgatgata taaaattgcc atcatttatt
     2281 gaagagattg atagtatagg taaggtttta aacgatgctg atattgattt tacgtttgaa
     2341 aataaagaat tggcgcaagt attgagccct accaaacaat ccatgttagt tatgattgcg
     2401 cgtgaagcga taaataatgt aattaaacat gcgaatgctt caaaagttca tggcaaatta
     2461 aaaacagtaa acaatcataa attactgctt atgattgaag atgatggcaa aggtatcgat
     2521 agtggttgtg aggtgaaaag tatttcacag cgtgtacaac atttaaatgg aactttagca
     2581 gtcgactcaa caaatggaac taaaataatc atcgaaatct caacaggagg aatagtatga
     2641 tatctttaat tatagcggaa gatcaaaata tgttgcgaca ggcaatggtt caattaatta
     2701 aactacatgg tgattttgaa attttagcag atacttataa tggtcttgat gcaatgaaac
     2761 ttattgaaga atataatcct aacgttgtta ttttagatat agaaatgcca ggcatgactg
     2821 gacttgaagt tttagcggaa attagaaaga agcatttaaa tattaaagtg attattgtaa
     2881 caacttttaa aagaccggga tactttgaaa aagcagttgt gaatgatgtg gatgcatatg
     2941 ttttaaaaga acgttctata gaagaattgg tggaaaccat taataaagta aataacggag
     3001 agaaagaata tagcgccaca ttgatgactt cattttttgt agataaaaac cctttgacac
     3061 caaaagaaca aattgtatta agggaaattg gcaatggttt aagtagtaaa gaaataagcg
     3121 aaaaattatt tttgacagat ggaacagtta gaaattatac atctaccata attgataaat
     3181 tatttgcaga taatcgtttt gatgcttgga aaaaggcgaa tgaaaaaggt tggatataaa
     3241 ttaaaataaa aagt
//
LOCUS       DAHHRR010000178         3245 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.182, whole genome shotgun sequence.
ACCESSION   DAHHRR010000178 DAHHRR010000000
VERSION     DAHHRR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.182"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(285..1055)
                     /gene="sigB"
                     /locus_tag="NBI42_002522"
     CDS             complement(285..1055)
                     /gene="sigB"
                     /locus_tag="NBI42_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HCD1778907.1"
                     /translation="MAKESKSANEVSPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHK"
     gene            complement(1030..1509)
                     /gene="rsbW"
                     /locus_tag="NBI42_002523"
     CDS             complement(1030..1509)
                     /gene="rsbW"
                     /locus_tag="NBI42_002523"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HCD1778908.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKEKNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            complement(1511..1837)
                     /locus_tag="NBI42_002524"
     CDS             complement(1511..1837)
                     /locus_tag="NBI42_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HCD1778909.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            complement(1956..2957)
                     /locus_tag="NBI42_002525"
     CDS             complement(1956..2957)
                     /locus_tag="NBI42_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HCD1778910.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
BASE COUNT         1012 a          617 c          388 g         1228 t
ORIGIN      
        1 gattattgaa aatgacacac catcatttcc taaatttcat aatctaagcc tactttttta
       61 ttctaatagc aatccactta attaaaccga caattagata tcttcttcaa ttaaattatt
      121 aatactatat cgcgacattt atgtggatac acattaaact acgttaataa tgcattaatt
      181 cgaaaatctt aattaaatta aaaatacccc tcgatttcaa tttaataatc aagggataat
      241 tatatggatt tttcattata aaacgcatca ttaataagca aattctattt atgtgctgct
      301 tcttgtaatt tcttaattgc cgttctttga agtcttgaaa catgcatttg actcaaaccg
      361 atacgctcgc ccgtttcttt ttgactcaaa ccttcaataa acgtacattg tatgatttct
      421 cgttcgcgat cagataatat aggtaatatt ttttctaaaa tcatacgctt ttcagttaag
      481 tcataatggt catcttgttg tcccataata tctaatagcg taacagttga accatcttta
      541 tcagcttcaa tggaatgatc aacacttaac gcattataac tttgtcccat ttccattgct
      601 tctaaaactt cttcttctga aacttctaat cgatctgcta tttcactgat agaaggtgaa
      661 cgctctaatt cagcggttag ttcatcgctc acttttttga ttcttggccc aatttcttta
      721 atacgtctcg gaacatgtac actccaagtt ttatctcgta gatatctttt gatttcaccg
      781 attacagtag gtactaaaaa ggcttcaaac ttccgttcaa aggacatatc gaatctattt
      841 atggcaccta ttaaaccaac cataccaact tgaactaaat cttcgtgatg tgattgtcct
      901 ttagaatatt tatatgccaa tgactcaatt agtttttggt aatgtttaac taacttatcc
      961 tgtgcatctg tattcttatt ttcttggtgt tctttaatcc attggttaat ttgctcaggt
     1021 gaaacttcat tagctgattt cgactctttc gccattattt cgcacctgct ctttttttat
     1081 atacttagtc atactgattg tcacaccaga ttctttatat actgtcactt catccattaa
     1141 agattcgatt aaaaataaac ctaggccacc ttcgcgtaaa aagtctatat tttcgtcttt
     1201 atcgtaagga cctatttttg atttagttgt ttcataatca aaactgtcac ctttatcaga
     1261 aataacaatt ttaattttat cttctaaaat ttcaaaatat atgttaataa tgcccacatt
     1321 gtttttttct ttgtatgcat gtttaactgc atttgtcaca gcttcactaa ctgcaatctt
     1381 ggcatcttca atatcatcat atgtagcacc agctctcgaa aaaacgccag aaagtgttaa
     1441 acgaattaaa cttacatact ctgccgatgc tggcacgcgc atttcgataa aatcttcttt
     1501 agattgcatg ttattcgacc tccgttcctt cattaacatg cattaaatcc ttaagaccag
     1561 taatttcaaa tagtctaccg atacgatctg acacacctaa aatgtatagt tctttatcat
     1621 tttggtttaa tgcttttaat gtacctacga ataaacctaa acctgtcgaa tccatataac
     1681 tcacattttc taaattaaca taaatatcac gagttccatc ttgtctcata ggtgttaaaa
     1741 cctcttctaa ttcaggcaca gtataaacat ctaattctcc accgacttta acttcgtaaa
     1801 atttatcttg agtggttgtt tctatattaa gattcatttc attacactcc tacttattaa
     1861 aaatatttat cttcatttta agcgctgtat ccaccatatt tatatttcaa attattatat
     1921 acccatcgaa ataatttcta atcttctttt ttaaattaat ttactctttt tataatcaaa
     1981 atagtcatat catctttttt atttgggttt tgaagcttta aaattgcttc atagataatt
     2041 tgaacaatat cttgtgggtg catatgttta tgttttttaa tatattctaa aagtttttgt
     2101 ttatctataa aggtaccttc actatttcta gcttcagtca caccatccgt taaaatgata
     2161 attaaatcat caaggtatat tggaatttct tgctgttgat atcgtgtttg tgaactgatt
     2221 cctaacactc tacctctaac tgaaatttct tcaaattctt ctttttcagc gcgataaata
     2281 tatccaggct catgaccagc tgaactacaa tacaataaat ggttcatttc ttcatataaa
     2341 ccataaaaca ttgtgacgaa catattttga ttaatattct tttcaacaac acgatttaaa
     2401 cgttttaagc catcactcgg taattgtgag tgtccataag aatccatgcc aaactttatc
     2461 atactcattg ctaaagcagc tggtatacct tttccaatga catctgcaac agcaaagctc
     2521 attgtgccat cgttatggtc aattaaatta aaataatctc cacttacttt ttgtgccgcc
     2581 actgaaataa cgccaatttg aatactatca aattgtggaa tatctgtttt aagcattgtt
     2641 tgttgtaagc tagaagctaa gtctatctct ttatcgtgaa cttgaagttt atctaccaat
     2701 ctttgataat ctcgataact ataaccaaag cctttaacga tttcttgtaa gacatctaat
     2761 gtcttgaaca catcttcacg cgttaagttt aacgtcaata tatagttttt gtgaatatcg
     2821 acaatgtctt caggcaagac gtccttacga atcacttcgt cagtgtattt ctcacacttt
     2881 tttatcaatt ctactttatc ttggcacgtt aaactttcat caattaaacc cttataatgt
     2941 tgcttaaatt cttccacgat tagttgcctc ctcacttccc ttacaaagca aatgcttaag
     3001 caaggataat tatgattcac ttactaacat ttatttaatt gcacttatca attttgtcgt
     3061 ttgcaatttt gctgtagatg atacttttaa gctaaaaaaa actttaggat ggaataccta
     3121 aagcctaaaa aacttaagac atccttttat aataaaaaat atgaatatca taactggtat
     3181 ttgctaaaat ctttgtttat aagaaaattc ttcgtacaga atttagaaat agttataatt
     3241 attat
//
LOCUS       DAHHRR010000179         3218 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.183, whole genome shotgun sequence.
ACCESSION   DAHHRR010000179 DAHHRR010000000
VERSION     DAHHRR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.183"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..1920
                     /gene="rsp"
                     /locus_tag="NBI42_002526"
     CDS             <1..1920
                     /gene="rsp"
                     /locus_tag="NBI42_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HCD1778911.1"
                     /translation="NIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIADLIS
                     TPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNIDAHL
                     SLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTVSELA
                     GFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTIDLTAQ
                     LATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPVVFID
                     DIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQDYKT
                     SKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQVYDVM
                     QRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVYNNLS
                     VSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSPLPLM
                     HLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNELPQD
                     SLMIIKTLNHEHGSIQHLLPTSDQLVYIEKEILDELDKTNYPKTELAVQEETGRTFEL
                     KLNHDEVKYICFKPS"
     gene            1991..2413
                     /locus_tag="NBI42_002527"
     CDS             1991..2413
                     /locus_tag="NBI42_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCD1778912.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKINL"
     gene            2693..3049
                     /locus_tag="NBI42_002528"
     CDS             2693..3049
                     /locus_tag="NBI42_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCD1778913.1"
                     /translation="MKNKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     ATIEKMISEKDESIEKNVKLPSDADVSVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
BASE COUNT         1186 a          474 c          464 g         1094 t
ORIGIN      
        1 aatataatcg aattaatgat acccgttttt tatttttatc aacaagatga tgatttcttt
       61 aatggttatt tagatcgtca tttattacaa tcaagtaatt atattaagtc attaattgcc
      121 gatttaatca gtacaccaac aagttcttct ttaatgggga aaaatatagg ccaaagtatt
      181 atagacactt tacttaaaga agcttttata agaatagatc atgaatattt accaaatatt
      241 gctttaagca atccggtgtt tatagactgt gtaaactata ttcatgacaa tattgatgcg
      301 cacttgtctt taaaagatat agcgatgcat tgcaacatct ctgaatcata ttgttctaat
      361 ctttttgttc gatatttgag tatgaatttt aaagattatt ttacaagtat taaacttgtt
      421 aacgccatca atttattact ttctacaaaa cattctatta caactgtttc tgaattggcc
      481 ggttttaaca gtcatacaaa ttttgcaaat caatttaaga attacttgca ttttagtcct
      541 aaacagttcc gttcactcgt ttccaagatt actgaaccac cacaaataca cttccaacaa
      601 gataacgtat cacaattcac tgagttaatt tcaacaattg atttaacagc tcaactggct
      661 acaaatacaa cagatattca tatagatgat tttaatccga aagaccgaag tcagcgtgcc
      721 aaagtatttg ttcgatttag taatttcaat gaactatttc aatttatttt caatgaatat
      781 tacgatatta actttgagca cttaccaaaa cctgtcgttt tcattgacga tattcacgat
      841 attgagatta gccaaacgaa ttacaacctg ttaaatagat gttttgaaaa gcttttcgaa
      901 aaaaatatag gtttagcaat cgctattaaa tcaacacaac aatttgaaac gatgaagcaa
      961 ttaatactaa catttttaca aggtaatcaa gactataaga caagcaagaa attagttaaa
     1021 tttatgctcg tattttgctc taattctatg acagcagaag aaattcattt atgtcatctt
     1081 aaaattaaaa acaaaaataa agaaattaaa tatagtgtga cagtcgatgg ctttttagaa
     1141 acttattcaa ccgtggaaca agtttatgat gtcatgcagc gtcttaaatt ccattattac
     1201 tttattgata ttgaaaattc aaaaactgca acacatctta tcactaaaaa ccaacactat
     1261 catcaaactg acactcattt tgaacagtat aagaaattca ttttagattc aggtatatca
     1321 tcaacacaat tcgtatataa taacctgtct gtaagcggat ttaaatatac taatgatggt
     1381 aagaatccaa ttcaattatc tgacatagtg tatcacttaa tcgcattatt acgatatggc
     1441 ggtggcatta gctatcaatt attagatgac cattcaaatt atatttcctt gtacaacaaa
     1501 tatggtagcc ccctaccatt aatgcattta tataaaatgt ttagaccttt tgttaatgaa
     1561 gacattgaaa ttacaaataa ttatgtattg agtcgtaaag ataataacta ccatttctta
     1621 ttattcaata aaattaatga ccgatatatg tcagacgtaa aacaagactt cattttccat
     1681 aatgaattac ctcaagattc tttgatgatt attaaaactt tgaatcatga acatggttca
     1741 attcaacatt tgcttccaac aagcgatcaa cttgtctata tagaaaaaga aattctagat
     1801 gaattagaca aaacgaatta ccctaaaacg gagcttgcag ttcaagaaga aactggtcgt
     1861 acatttgaac tcaagttaaa tcacgacgag gttaaatata tttgctttaa accaagctaa
     1921 atactaacag tcctcttgtg tttagtttct tacgttaaag gctatttata tcataaggag
     1981 atgatatagt atgagtaact cacaagcaat tcaagcaatt gaaaacgtgt tagcaacgtc
     2041 aaaagttggt gtattatcaa ctgcatataa taacaaacct aatagtagat atatggtctt
     2101 ttataatgat ggtcttactt tatatactaa aacgaatatc cattctgcta aggtcaaaga
     2161 aattaaagat aatccagcag catatgtttt gttaggctat aacgacacaa ctaatcgtag
     2221 tttcgttgag atggaagcga caatcgaaat cgttacagaa caagaagtga ttgattggct
     2281 atgggaaaca caagacaaaa gctttttcag ttcaaaagaa gacccagagt tatgtgtttt
     2341 aagagtagtt ccgcaatcca ttaagctaat gaatgataaa tcattagata cacctattaa
     2401 aattaattta taatatgtaa gttttttagt tttaaaaatt agccagcaat tgaacatatt
     2461 agctataaat ttcaacagta tcctatttat atatatttcg cttttgaaag cgtttaatac
     2521 ttttaactat atttttatta tataacaata ttttagacac attttattca catttattgt
     2581 gattaaggtg tgtcttttta aattaatttt atgtaatata aatactgcat ttgcaaactg
     2641 ttgtactttt aggtataaca gaattaacta catttaagga gattgatgaa ccgtgaaaaa
     2701 taaaaaaggt tttggtcttg gtattagttt aattgccatc atgttgattg tatgtattgt
     2761 actagtaatc atgatgatga ctggtggaaa gaaagataca tattatggaa ttatgaaaga
     2821 caatgctaca attgaaaaaa tgattagtga aaaagatgaa agcattgaga agaacgttaa
     2881 attaccttca gatgcagacg tttcagttaa aaaaggtgat tttgtaattg tttataaatt
     2941 agcagattct gataaaattg ttaaagtaaa aaaagttgat catgacgatg taccacatgg
     3001 tttaatgatg aaaattcatg acatgggcaa aatgcacatg aaacactaat taagctttaa
     3061 ttttgagtaa tatataagat attaaaagca gcagtaagat attttctaat tgaaaattat
     3121 cttactgctg ctttttaaga attactgtgt cagtctattt ccggttcgcc tgcgctttgc
     3181 gcatgcataa aagcccctaa caaccagagg tcccttcg
//
LOCUS       DAHHRR010000180         3165 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.184, whole genome shotgun sequence.
ACCESSION   DAHHRR010000180 DAHHRR010000000
VERSION     DAHHRR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.184"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            146..2755
                     /gene="adhE"
                     /locus_tag="NBI42_002529"
                     /gene_synonym="adhC"
     CDS             146..2755
                     /gene="adhE"
                     /locus_tag="NBI42_002529"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCD1778914.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKAIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQRTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPEKATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
BASE COUNT         1187 a          475 c          587 g          916 t
ORIGIN      
        1 atgaaaacat ttattttaaa atttgatatt tgttcaaata atattcgaaa ttaaactttt
       61 ttgtatagaa ttttctttat atcctgagag acatgtacta taatgtttgt gaaataattc
      121 acaaagtata aaggagtggt tgtatatgtt aactatacct gaaaaagaaa atcgtggatc
      181 gaaagaacaa gaagtggcaa ttatgattga tgctctagct gacaaaggga aaaaagcatt
      241 agaagcatta tctaaaaagt cacaagaaga aattgatcat attgttcatc aaatgagctt
      301 agcagctgtt gatcaacata tggtgctagc aaaattagca catgaagaaa ctggaagagg
      361 tatatacgaa gataaagcga ttaaaaattt atacgcttct gaatatatat ggaattcaat
      421 aaaagacaac aagacagtag ggattattgg tgaagataaa gaaaaaggat taacgtatgt
      481 agcggaacca attggcgtta tttgtggtgt tacgccaaca acaaatccta cgtcgacaac
      541 tatttttaaa gcgatgattg caattaagac aggaaatcca atcatttttg cattccatcc
      601 aagtgcgcaa gaatcgtcga agcgtgcagc agaagttgta ttagaagcgg caatgaaggc
      661 aggtgcacct aaagatatta ttcaatggat tgaagtgcct tctatcgaag caacaaaaca
      721 attaatgaat cacaaaggta ttgcattagt tctagcaaca ggtggttcgg gcatggttaa
      781 gtctgcatat tcaactggca aaccggcatt aggtgtggga ccaggtaacg tgccgtctta
      841 cattgaaaaa acagcacaca ttaagcgtgc agtaaatgat atcattggtt caaaaacatt
      901 tgataatggt atgatttgtg cttctgaaca agttgtagtc attgataaag aaatttataa
      961 agacgttact aatgaattta aagcacatca agcatatttt gttaaaaaag atgaattaca
     1021 acgcttagaa aatgcaatta tgaatgaaca aaaaacaggt attaagcctg atattgtcgg
     1081 taaatctgca gttgaaatag ctgaattagc aggtatacct gtccccgaaa atacaaaact
     1141 tatcatagcc gaaattagcg gtgtaggttc agactatccg ttatctcgtg aaaaattatc
     1201 tccagtatta gccttagtaa aagcccaatc tacaaaacaa gcatttcaaa tttgtgaaga
     1261 cacactacat tttggtggat taggacacac agccgttatc catacagaag atgaaacatt
     1321 acaaaaagat tttggactaa gaatgaaagc ttgtcgtgta cttgtaaata caccatcagc
     1381 tgttggaggt attggtgata tgtataacga attgattccg tctttaacat taggttgtgg
     1441 ttcgtacggt agaaactcaa tttcacataa tgttagtgca acagatttat taaacattaa
     1501 agcgattgct aaacgacgta ataatactca aattttcaag gtacctgctc aaatttattt
     1561 tgaagaaaat gcaatcatga gtctaacaac aatggataag attgaaaaag tgatgattgt
     1621 ctgtgaccct ggtatggtcg aattcggtta tacaaaaaca gttgagaatg tattaagaca
     1681 aagaacggaa caacctcaaa ttaaaatatt tagcgaagtc gaaccgaacc catcaacgaa
     1741 tacagtatat aaaggtctgg aaatgatggt tgatttccaa ccggatacaa tcattgcact
     1801 tggtggtggt tcagcgatgg atgctgcaaa agcaatgtgg atgttctttg aacaccctga
     1861 gacatcattc ttcggtgcta aacaaaagtt cctagacatc ggtaaacgta cttataaaat
     1921 aggcatgcct gaaaaagcga cgttcatttg tatccctacg acatcaggta caggttcaga
     1981 agtaacacca tttgcagtta tcacagatag tgaaacaaat gtaaaatatc cgttggctga
     2041 ttttgcttta acacctgatg ttgcaattat tgaccctcaa tttgtgatga gtgtgccaaa
     2101 aagcgttaca gcagatacag gaatggatgt actaacgcat gcaatggaat catatgtatc
     2161 tgtaatggct tcagattaca caagaggttt gagtctacaa gcgattaaat tgacgttcga
     2221 atatttaaaa tcatctgttg aaaagggtga taaagtttca agagagaaaa tgcataacgc
     2281 atcaactttg gctggtatgg catttgcaaa tgcattctta ggcattgcac actcaattgc
     2341 gcataaaatt ggtggcgaat atggtattcc gcatggtaga gcgaatgcga tattactacc
     2401 gcatattatc cgttataatg ctaaagaccc gcaaaaacat gcattattcc ctaaatatga
     2461 gttcttcaga gcagatacag attatgcaga tattgccaaa ttcttaggat taaaaggtaa
     2521 tacgacagaa gcactcgtag aatcattagc taaagctgtc tacgaattag gtcaatcagt
     2581 cggaattgaa atgaatttga aatcacaagg tgtgtctgaa gaagaattaa atgagtcaat
     2641 tgatagaatg gcagagctcg catttgaaga tcaatgtaca actgctaatc ctaaagaagc
     2701 actaatcagt gaaatcaaag atatcattca aacatcatat gattataagc aataatctat
     2761 ctgataataa tcatctaact cacctgaaat tacaaaagta aaaaatgcca cataaacttt
     2821 aagtcgataa tcattatacg gttatcggct tttatttatt gccaaatctt cagagagata
     2881 caaactagac aatcattttt taaaataaag aaaatattaa gattgatact catttcacaa
     2941 actattacta ctttagagta taattatttt taatttcata taaataaaaa ggcgaaaata
     3001 atgctgttta aaagtaatta attgtttaaa cgatatgtaa tatgtaaata ctatatatat
     3061 aataccaatt ttaatgaaaa tttttaaggg aggtaaataa tggaaagtac attagaatta
     3121 acaaaaatta aagaagtatt gcaaaaaaac ttgaagattt taatt
//
LOCUS       DAHHRR010000181         3111 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.185, whole genome shotgun sequence.
ACCESSION   DAHHRR010000181 DAHHRR010000000
VERSION     DAHHRR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.185"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            18..2168
                     /locus_tag="NBI42_002530"
     CDS             18..2168
                     /locus_tag="NBI42_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCD1778915.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVTKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     KLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLKEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            2161..2616
                     /locus_tag="NBI42_002531"
     CDS             2161..2616
                     /locus_tag="NBI42_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCD1778916.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTTRMRCGHCNGKLRMKRQLK"
BASE COUNT         1198 a          402 c          594 g          917 t
ORIGIN      
        1 aagtgtgggt taagtaaatg gacaatcaat tgattaattc aatcatagag aaatatcaat
       61 ttagtaaaaa acaaattgaa gcagtgttaa cactgcttga agaaaaaaat acagtaccat
      121 ttattgcgag gtatcgaaaa gagcaaactg gtggactaga tgaagttcaa ataaagcaaa
      181 ttgatgacga ataccaatat atggtcaatt tacaaaaacg taaagaagaa gttatcaaaa
      241 atatagaaca gcaaggatta cttactgagg aattaaagaa agatatttta aaacagaaca
      301 aattacaacg tgttgaagac ctatatagac cttttaaaca aaagaaaaag actagggcaa
      361 ctgaggcgaa acgtaaagga ttagaaccat tagcgatatg gatgaaggca cgtaaacatg
      421 aagtctcaat tgaagaaaaa gcacaacaat ttataaatga agaagtgcaa tcggttgaag
      481 atgctatcaa aggtgcgcaa gatattattg cggaacaaat ttcagataat cctaaatata
      541 gaacaaaaat tttaaaagat atgtatcatc aaggtgtgtt aactacatct aaaaagaaaa
      601 atgctgaaga tgaaaaaggt atttttgaaa tgtactatgc atacagtgag ccaattaaac
      661 gcattgctaa tcatagagtt ttagctgtga atcgtggtga aaaagagaaa gtattatctg
      721 taaagtttga attcgatacg acatcagtag aggatttcat tgcacgtcaa gaaatcaatc
      781 ataataatgt aaatcgcagt tatattttag aggcgattaa agatagcttg aaacgcttaa
      841 ttgtcccttc gatagagcgt gaaatccatg ctgatttaac tgaaaaagct gaaaatcatg
      901 caatagatgt ttttagtgaa aacttaagaa atctattact gcaacctcca atgaaaggta
      961 aacaaatatt aggcgtagat cctgcattta gaacaggttg taaattagca gtcattaacc
     1021 catttggtac ttttatagca aaaggtgtga tttatccgca tccaccagtt actaaaaaag
     1081 aagcagcaga gaaggatttt gtacaaatgg ttaaagcgta tgatgtgcaa ttaattgcaa
     1141 ttggcaatgg tacagcaagt cgtgaaacag aacaatttgt tgcagattta attaaaaagc
     1201 atcagttgcc agtacaattc atcattgtca atgaagcggg cgcttcagta tactcagcat
     1261 cagaaattgc tagagatgaa tttcctgatt ttcaagtgga agaaagaagt gccgtatcaa
     1321 ttggacgacg cgttcaagat ccattaagtg aattagttaa aatcgatcct aaatctattg
     1381 gtgttggaca ataccagcat gatgttaatc aaaaagcact agaaaatgca ttaactttcg
     1441 ttgttgaaac agcagttaat caagtaggtg ttgatgtcaa tacagcatca tcatcattat
     1501 tacaatatgt gtctggtcta agttcgcaaa ttgctaaaaa tataattgca tatagagaag
     1561 aaaatggcgc aataaagcat aataaagagt taagcaagat taagcgtttg ggtgctaaaa
     1621 cttttgagca gagtatagga tttttaagaa tagtagacgg gtcagagcca ttggataata
     1681 catcgattca tcccgaaagc tataaagtaa cgtataagtt attagataaa ctcggatttg
     1741 gtggcaatga tttaggtagt gatgcattaa aagctaaatt aaattcattg gatatggatg
     1801 aactggcgat tgaattacaa gtaggtgtac ctacattaga ggatattatt aaatccttga
     1861 aagcacctaa tcgagatcca agggacgagt ttgaaacacc aatccttaaa tccgatgtgc
     1921 tatcaattga agatttaaaa gaaggaatga aattgagtgg cacagttaga aatgtagtag
     1981 attttggagc atttgtagat ataggtgtta aacaagatgg ccttgtccat gtttcgaaac
     2041 tttctaagaa gttcgtaaaa aatccaatgg atattgtcag tgtcggtgat atcgttgacg
     2101 tttggattta cagtattgac aaaaataaag acaaagtatc attaacgatg attgatccac
     2161 atgaataatg acaaacttca gcgaatggtt gagaaccttt cggaagaaaa atttggacgt
     2221 acgtttcgac attgtgcata ttttaataaa aggttacgta caacaggcgg acgttatctt
     2281 ttaaagtccc atgatattga aattaatcca aaacaatatg agcattatgg agaagatgct
     2341 gttgtaaaaa ttatactgca cgaattatgt cattatcact tgcatattgc tggtaaaggt
     2401 tatcaacata aagatcaaga ttttaaacga ttgagccaac aggttggagc gcctagattt
     2461 tgtaacagca ttgaaagcta tcaacaacgg gcgaattatg aatattattg cactaaatgt
     2521 catgcaaaat atatacgtat ccgtaaagtt gatacgactc gtatgcgttg cggacattgt
     2581 aatggaaaat taagaatgaa aagacaattg aaataagtac aatacaaata gagctaattc
     2641 gagtatatgt aagattattt atgagttgta tgaaatttat atagaattta tcagtgagtt
     2701 attttgattg gtaaacatag taattgaata agaagaaatt gtgtttataa aatgtttaaa
     2761 gccgagattg tcgtaattta atacttcgca atcgcggctt tctacttttg atataggtaa
     2821 tggcataaat tgtgacttga taggatgaaa ttcaattgat tagattaaaa atcttggcgt
     2881 attttagtaa tgaataatat ataaacttta cccaattaaa gctttaaagt agcacaaaag
     2941 ataattatac atttttaatg acattttgat tgaatttatc tattgactgt acagcttata
     3001 gttagtatga ttaaacacgt tccttaaaaa agaacaaatt aaataaactt tgatttgaaa
     3061 gacagtcaat tctaaattta atttataaaa tatgttgaca ataagttctt a
//
LOCUS       DAHHRR010000182         3104 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.186, whole genome shotgun sequence.
ACCESSION   DAHHRR010000182 DAHHRR010000000
VERSION     DAHHRR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.186"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..1374
                     /locus_tag="NBI42_002532"
     CDS             <1..1374
                     /locus_tag="NBI42_002532"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCD1778917.1"
                     /translation="EDITYQEAWTDEEYREDLKAELIDQVGYFIEPQDLFSAMIREIE
                     TQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVM
                     INLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGK
                     DKLRHVYDPTCGSGSLLLRVGKETKVYRYFGQERNNTTYNLARMNMLLHDVRYENFDI
                     RNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFI
                     QHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSI
                     PTCILVFKKSRQQDDNVLFIDASNDFEKGKNQNHLTDTQVDRIIDTYKRKETIDKYSY
                     SATLQEIADNDYNLNIPRYVDTFEEEEPIDLEQVQQDLKNIDKEIAEIEQEINAYLKE
                     LGVLKDE"
     gene            1367..2605
                     /locus_tag="NBI42_002533"
     CDS             1367..2605
                     /locus_tag="NBI42_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD1778918.1"
                     /translation="MSNTQKKNAPELRFPEFEGEWKEKKLEDTLEFIKDGTHGTHENV
                     NNGPWLLSAKNIKNNKIIISSDDRKISESDYKKIYKNYKLEKGDLLLTIVGTIGRAAI
                     VKNPNNIAFQRSVAILKTKATYDVGFIFQLFQTKYFKNLLLRKQVVSAQPGLYLGDIR
                     KIKISITNIIEEQRKIGIFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDE
                     NGNDYPKWEEKKIEDIASQVYGGGTPNTKIKEFWNGDIPWIQSSDVKVNDLILQQCNK
                     FISKNSIELSSAKLIPANSIAIVTRVGVGKLCLVEFDYATSQDFLSLSSLKYDKLYSL
                     YSLLYTMKKISANLQGTSIKGITKKELLDSIIKIPHNLEEQQKIGDLFYKIDKYISFN
                     KCKIEMLKSLKQGLLKKMFI"
     gene            2642..>3104
                     /locus_tag="NBI42_002534"
     CDS             2642..>3104
                     /locus_tag="NBI42_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778919.1"
                     /translation="MEKLNEAIKNYIDSDELFALFIDGQWGVGKTYYIKNKILKEFDD
                     KIEIRYISLYGLQSLYEIKSIIISKLINTSTKNLRKVLKSMKNFQVVPYLNMNNFSDN
                     ILRYIDDNSLKKIKNSLMKNNKSALLIIDDVERMSDKVSFEEFLGFIRNDLI"
BASE COUNT         1231 a          423 c          574 g          876 t
ORIGIN      
        1 gaagatatta catatcaaga agcatggaca gatgaagaat accgtgaaga cttaaaagca
       61 gaattaattg atcaagtcgg ttacttcatt gagccacaag atttattcag tgcgatgatt
      121 cgtgaaattg aaacgcaaga tttcgatatc gaacatctgg cgacggcaat tcgtaaagtt
      181 gaaacatcaa cactaggtga ggaaagtgaa aatgacttta tcggactgtt cagcgatatg
      241 gacttgagtt caacgcgact aggtaacaat gtgaaagaac gtactgcgtt aatttccaaa
      301 gtcatgatta atcttgacga tttaccattc gttcacagcg acatggaaat agatatgtta
      361 ggtgatgcat atgaattcct tatcgggcgc tttgcggcga cagcgggtaa aaaagcagga
      421 gagttctaca caccacaaca agtatctaag atactggcga agattgtcac agacggtaaa
      481 gataaattac gtcacgtgta tgacccaaca tgtggttcag gttcattatt gttgcgtgtt
      541 ggtaaagaga caaaagtgta tcgttatttt ggtcaagaac gtaacaatac cacatacaac
      601 ttagcacgca tgaatatgtt attacatgat gtgcgctatg aaaatttcga tattcgtaat
      661 gatgacactt tggaaaatcc agccttttta ggcaatacat ttgatgcggt aatagcgaac
      721 ccaccatata gtgcgaaatg gacagctgat tcaaagtttg aaaatgacga gcgatttagt
      781 ggttatggta aacttgcgcc aaaatccaaa gcagactttg cctttattca acatatggta
      841 cattacttag atgatgaagg taccatggca gtcgtactcc cacatggtgt attattccgt
      901 ggtgccgcag aaggtgtcat tcgtcgttat ttaattgaag aaaagaacta cttagaagcc
      961 gtgattggct taccagcgaa tattttctat gggacaagta taccaacatg tatccttgta
     1021 tttaaaaaaa gtcgccaaca agacgacaac gtattattta tcgatgcatc caatgatttt
     1081 gaaaaaggaa aaaatcaaaa ccatttaacc gatacccaag tcgatcgcat tatagacaca
     1141 tataagcgta aagaaaccat tgataaatat agctacagcg cgacattaca agagattgcc
     1201 gataacgatt acaacttaaa tataccgaga tatgtcgaca cattcgaaga agaagaacca
     1261 attgatttag agcaagtcca acaagatttg aaaaatatcg acaaagaaat tgcagaaatt
     1321 gagcaagaaa tcaatgcata cctgaaagaa cttggggtgt tgaaagatga gtaatacaca
     1381 aaagaaaaat gcgccagagt tgagattccc agaatttgaa ggcgagtgga aagagaagaa
     1441 gctagaagat acactcgaat ttataaaaga tggaacacat ggaactcatg aaaatgttaa
     1501 taatggacca tggttattaa gtgcaaagaa tatcaagaat aataaaatta taatttcaag
     1561 tgatgatagg aagatatcgg aaagtgatta taaaaaaata tacaaaaatt ataaacttga
     1621 aaaaggcgat ttattattaa caattgtagg tacaattgga agagcagcta ttgtcaaaaa
     1681 tccgaataat attgcatttc aaagaagtgt cgctatttta aaaacaaaag ccacatatga
     1741 tgtagggttt attttccaac tttttcaaac aaaatatttt aaaaatttac tgttaagaaa
     1801 acaagttgta tctgcgcagc caggattata cttaggtgat attcgaaaga taaaaatttc
     1861 aattacaaat ataattgaag aacagcggaa aattggaatc ttcttcagca aactcgatcg
     1921 acaaattgaa ctagaagaac aaaaacttga attacttcaa caacagaaaa aaggctatat
     1981 gcagaaaatt ttctcacagg aattacgatt caaagatgag aatggtaatg attatccaaa
     2041 gtgggaagag aagaaaatag aagatatagc aagccaagta tatggaggtg gaacaccaaa
     2101 tacaaaaatc aaagaatttt ggaatggtga tattccatgg attcaaagct ctgacgtaaa
     2161 agtaaatgat ttgattctac aacaatgtaa taaatttatt tccaagaatt caattgagct
     2221 ttcttctgca aaacttattc ctgccaattc aattgcaata gttacaagag tcggggttgg
     2281 aaaactgtgt ttggtagaat ttgattatgc tacaagtcaa gattttttat cattaagtag
     2341 tcttaaatat gacaaattat actcattata ttcattgcta tacacaatga aaaaaattag
     2401 cgctaatcta caaggaacct caattaaagg tataacaaaa aaagagttgt tagatagtat
     2461 aataaagata ccccataatc tagaagaaca gcaaaaaata ggtgatctat tttataaaat
     2521 tgataaatat atcagtttta ataaatgtaa aattgagatg cttaaaagtc tcaaacaagg
     2581 attacttaaa aaaatgttta tataaatgat atagaatttg aaattttgta gaggtgaatt
     2641 gatggaaaaa ttaaatgaag caattaaaaa ctatatagat agtgatgaat tgttcgccct
     2701 ttttatagat ggacaatggg gagtagggaa aacttattat attaaaaata aaatattaaa
     2761 ggaatttgat gataaaatag aaattaggta tataagttta tatggcctgc aaagcttata
     2821 tgaaattaag agtataatca taagtaaatt gataaataca tctactaaaa atcttaggaa
     2881 agtcctaaaa agcatgaaaa atttccaagt agtaccttat ttaaatatga ataacttttc
     2941 tgataacata ttacgttata ttgatgataa ttcattaaaa aagataaaaa attccctgat
     3001 gaaaaataat aaaagtgcgt tgctaataat cgatgatgtt gaaagaatga gtgacaaagt
     3061 aagttttgaa gaatttttgg gatttattag aaatgattta attg
//
LOCUS       DAHHRR010000183         3093 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.187, whole genome shotgun sequence.
ACCESSION   DAHHRR010000183 DAHHRR010000000
VERSION     DAHHRR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.187"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..1155)
                     /locus_tag="NBI42_002535"
     CDS             complement(<1..1155)
                     /locus_tag="NBI42_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCD1778920.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQEINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVF"
     gene            complement(1578..2873)
                     /locus_tag="NBI42_002536"
     CDS             complement(1578..2873)
                     /locus_tag="NBI42_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778921.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            3025..>3093
                     /gene="graF"
                     /locus_tag="NBI42_002537"
     CDS             3025..>3093
                     /gene="graF"
                     /locus_tag="NBI42_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HCD1778922.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKD"
BASE COUNT         1103 a          505 c          401 g         1084 t
ORIGIN      
        1 aaaaacagct tttaaatatt tagatggctt ataatgtgga tgcgttttaa aatcttttgg
       61 taatcccata acttcagtta attcataatc aacgccactc tcttcaagtg tctttttaat
      121 agtattttta aattgcttta atggatatac acttgcgttt gtacataata ataatgttcc
      181 ttcagatgat aagatattta aggcgccatt aattaatttg tcataatctt tttgcactga
      241 aaatgtacgt tttttattgc gtgcaaagct aggtggatca atcacgatcg tgtcataact
      301 atgtccatgt cgtgcagcat atttatagaa atcaaaagtg tccatgacat aaatatattg
      361 agatttagga tcaatagcat ttagtccaaa attttcttca gttaaactac gagaacgatt
      421 agccaaatct acacttgttg ttgaagatgc ctcacttgct gctattacag aaaaagcacc
      481 tgtataacta aataagttta aaacatggcg ttctttggca tattgatccc ttaatttctt
      541 gcgcacttct ttttgatcta aaaagatacc tgtcatcaaa ccatcttcaa ggtctacatt
      601 ataaaatgtg aagttttctt cgataacaat tggaaactca ggtgcatcgc cttcaacaaa
      661 accaccacta tattcgctgt ctttaaatct tactttttcg taaatagatt tataatcaaa
      721 tacttttcta accgcttcaa gaatggcata tttaaattta taaatacctt ttgagtacca
      781 ttgaatcaac aaatggccat cgtaattatc gatcgttaat cccccaacac catcaccttc
      841 agcattaaat aatctaaaag catttgttcc atctatatta aaataataat cacgttctgc
      901 taatgcagta ttaaacaatt tcacaaagaa agctgtgttg atttcttgag ctttatcgta
      961 agttagaacc caacctaatc ctttatgttg gcgaccaaca taagccgttg caacatattg
     1021 tgatttatca gtcacaattt gaaaaatatc tccttctttt aaatgatctt gtgaatagat
     1081 atcctcttct tcaattaaag gatatccatt aaaatatttt gtttctttgc ctttgttcag
     1141 agttgctatt ttcatctgac aacttccttt ctgtgtttcg ttgtagtttt atatagatta
     1201 aattgtttgc caaatttcaa tatcatctac ttctaatctt actgttgatt ttaacaaatt
     1261 agtaattcga atgatatatg ttaaatttct acaaaggaaa tactatatat cgtttcgcaa
     1321 attacatatg tcgcttaaac tatttactag ttttcatgat tttacaaatg gataactttt
     1381 caactatttt atttgcacta aagctttgta tacaattttc aatacttaat tcacatcacc
     1441 tgagtcaatc aaatctagtt aaaaaaagta ctatccataa acttcattga cctgtatcta
     1501 agtaagttag agatatatct aacttatagc gtacacatca aaatgtttaa tagatagtac
     1561 ttttataatg cttttaatta tggtcgctct cgtaattgta acgaataaga aatgatgatt
     1621 gccaataaca ttaatagtaa tgttggcgta atgaacactg aaagtagtag tccatgaatc
     1681 attatattta taaattccgt taacaattta aagaataaaa tgctccccat aaatagttgt
     1741 aaataataaa acttaccttt caagtggaaa gttaacgttg tagcgatata tgcgataata
     1801 atgaatagca gaattagtaa tgtaacccat tcaataacat attgtgtctg tgataatttc
     1861 caatcaccag atacaaataa tgcattacga ttattaaatt ctaaaaatgt gaataaaaag
     1921 atgacgacac cacaaaatag ttcgacatac attggtttaa cccatttcgg ttttctcatc
     1981 caatcaggaa tatcgtcttc ttgaatatcg ataatatcat taactttctt tttgaattta
     2041 tctcttttag ctctaatttt atctaaatcg acacttcttc ttgtcttacg tgcatattct
     2101 gtagtcttat cttggagttg tttcaagtga attttagttt gattcgtgac attcccttta
     2161 cttgttcttg ataattctgg ctcctcttca cctaattctt gctttgttaa aggtaaagct
     2221 cggcgcaaga acaagaaatt ccaaatagcc atttgaccaa gtaaccacat aattataaag
     2281 aaagcattga cacttaagat atcaattgac tctatttcat tagcatcaat tctgccatac
     2341 aatgcaattt gtgtaattaa gtaacttata ccataactca caattatcca catgaaatga
     2401 ttttttacat tttcctgatt caattcatct ttatagacga tatatcctat atgaattgta
     2461 aatggaataa tgaaaatcac tgcaaagaat ccataaactt gagtcattaa tataagagta
     2521 ataataaagt acatgatacc gataactaag aaaaagatag aaatatcata gtcatactta
     2581 atagttctaa gcaatgtatg accaatcaac ataactaaaa taccgaatat aattatcaca
     2641 attgcactaa ctatagggaa attacctata aatcgactca ttactttaat aatttcactg
     2701 aaaaatgcat tcagaaattc ccacacatta tgaacatgtg tatcaacttt tttaccacca
     2761 tttaattgtt taattaatgg caagttaatt aaaataatga gaccagtgaa gagtgaaacg
     2821 ataccgatta aatagctaaa aataatttcg gcgtgtttcc taagaaaaga cattatgttc
     2881 acctcaaaat cattatatat taatctaatg ttgttgtctt tagataaatg aataaatagt
     2941 gctatagact tagaaaagtt taacgaaagt acaaccgggt aagatttata tataaataat
     3001 tatccaattt gggaggaatt aatcatgtca aatgaaaatc aaaataaaaa agcagcagaa
     3061 aaagctaaag aagttgaaga aaaattaaaa gat
//
LOCUS       DAHHRR010000184         3012 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.188, whole genome shotgun sequence.
ACCESSION   DAHHRR010000184 DAHHRR010000000
VERSION     DAHHRR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.188"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            125..697
                     /locus_tag="NBI42_002538"
     CDS             125..697
                     /locus_tag="NBI42_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HCD1778923.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDSYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNEYEISQAIVKIETLFPFITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            700..1425
                     /locus_tag="NBI42_002539"
     CDS             700..1425
                     /locus_tag="NBI42_002539"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HCD1778924.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            1427..2386
                     /locus_tag="NBI42_002540"
     CDS             1427..2386
                     /locus_tag="NBI42_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCD1778925.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQLSTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALDLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            2478..2927
                     /gene="ndk"
                     /locus_tag="NBI42_002541"
     CDS             2478..2927
                     /gene="ndk"
                     /locus_tag="NBI42_002541"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCD1778926.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGAKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT         1124 a          409 c          564 g          915 t
ORIGIN      
        1 tacataaatt actgttagtt taaattgaaa tttaaaaatg ataagtaatc ttaatgcttc
       61 aaaagatttt aatttcttac atataatgtt aatatgaata gtgataatat cgtgaggtgt
      121 agacatggaa acaactgtta gcaaattgga aagacaaata gaagaaagat taaagggcgt
      181 atcagaatat gaatccatta atattaatca tcggttaggt aagttactcg attcatatga
      241 tattccagat gttgccaaag tagcatgcct cacaattgac acatcaatga gacaccttga
      301 tgacattaca tataatcatt tgtctaagca ttccatattg attggagatt taattagtgc
      361 acatttttat acgctactag cagaaatcaa tgatttatca ttccaaaatg aaattagtaa
      421 agcaattgtt gaaattaatg aattgaaatc ttcattacat catcaagctt taaatgagta
      481 tgaaatttct caagcaatag taaaaattga aacacttttc ccatttatta cgttatctca
      541 tttcggtatc aatattgatg agtcggaaat atataattat ttatttgaag atatgtctga
      601 ttactatcca tcatacttca agaaatataa tcaaagtgaa gtaaagcatt acttacacga
      661 tatccaaaaa agttatttaa aaagtagagg taattaagca tggctgacaa taaagcaaat
      721 aaagagcaag tacatcgcgt ttttcaaaat atttcaaaaa aatatgacag attaaataat
      781 attattagtt ttgagcagca taaagtatgg agaaaacgtg tcatgaaaga catgggtgtt
      841 agaaaaggta cgaaagcatt agatgtttgt tgtggtactg gtgattggac aatcgcatta
      901 agcaaagctg taggaccaac aggagaagtt actggtattg actttagtga gaatatgtta
      961 gaagtaggaa aagaaaaaac tgcttcaatg gaaaatgtta aacttgttca tggtgatgca
     1021 atggaattgc cgtttgaaga caattctttt gattatgtaa caattgggtt tggattaagg
     1081 aatgtgccag actatttagt cgcgttaaaa gaaatgaata gagtacttaa accaggtggt
     1141 atggtggtat gtcttgaaac gagccaacca actttgccag tctttaaaca aatgtatgca
     1201 ttatacttta aatttgttat gcctattttt ggaaaattat ttgcaaaatc aaaagaagaa
     1261 tatgaatggt tacagcaatc tacgtttaat tttcctggaa aagaagagtt gaagcgcatg
     1321 tttgaagaag cgggtttcat aaatgtaaga gtacgtagtt ttacaggggg cgttgctgca
     1381 atgcaccttg gctataaaga aaaagataat accaaaggtg attaacgtgg caaagttaaa
     1441 catgaacaat gaaattaaga aagtggaaca acgacttgaa aaggcaatca aaagtaaaga
     1501 ttctgtatta gaacaggcat cattacactt attgtcttct ggtggtaaaa gagtacgccc
     1561 agcatttgta attctgagta gccaatttgg caaagatgaa caaacgtctg aacaaacgta
     1621 tcaagttgca gtcgcattag agttaattca tatggcaaca cttgttcatg atgacgttat
     1681 tgataaaagc gacaagcgtc gaggcaagtt aaccatatca aagaaatggg atcagacaac
     1741 tgctatttta actgggaatt ttttattggc attaggactt gaacacttaa tggccgttaa
     1801 agataatcgt gtacatcaat taatatctga atctatcgtt gatgtttgta gaggggaact
     1861 tttccaattt caagaccaat ttaacagtca acagacaatt atcaattatt tacgacgtat
     1921 caatcgtaaa acagcactgt taattcaatt atcaactgaa gttggtgcaa ttacatctca
     1981 atctgataaa gagactgtac gaaaattgaa aatgattggt cattatatag gcatgagctt
     2041 ccaaatcatt gatgatgtat tagacttcac aagtactgaa aagaaattag gtaagccggt
     2101 cggaagtgat ttgcttaatg gtcatattac gttaccgatt ttattagaaa tgcgtaaaaa
     2161 tccagacttc aaattgaaaa tcgaacaatt acgtcgtgat agtgaacgca aagaatttga
     2221 agaatgtatc caaatcatta gaaaatctga cagcatcgat gaagctaagg cagtaagttc
     2281 gaagtatcta agtaaagctt tggatttgat ttctgagttg ccagatggac atccaaaatc
     2341 actactttta agtttgacga aaaaaatggg gtcaagaaac acatagtatt tatgaaaagt
     2401 attgaaagcg ctttaccaac ctgttaatat ataatagtaa tatacataaa cctattaaac
     2461 acagggggat acataaagtg gaacgtacat ttttaatgat taaaccagac gcagtacaaa
     2521 gaaatctaat tggtgaagta atttcaagaa ttgaaagaaa aggactaaaa cttgtcggtg
     2581 ctaaattaat gcaagtacca atggaacttg ctgaaacaca ttatggtgaa caccaaggta
     2641 aaccatttta taatgattta atttcattta ttacatcagc accagtgttc gcaatggtag
     2701 ttgaaggtga agatgcagtt aatgtatcta gacatattat tggcagcacc aatccttcag
     2761 aagcttcacc aggatcaatt agaggtgatt taggtttaac tgttggtaga aatatcattc
     2821 acggttcaga ttcattagag tctgctgaac gtgaaattaa tttatggttt aatgaaaatg
     2881 aaattactag ctatgcttca ccacgtgatg cttggttata cgaataaaat aaaagctata
     2941 aacctttctg attaataaaa ggaaaaaggg ttttgttatg tggttagtca ttatgattat
     3001 acataacaag ac
//
LOCUS       DAHHRR010000185         2986 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.189, whole genome shotgun sequence.
ACCESSION   DAHHRR010000185 DAHHRR010000000
VERSION     DAHHRR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.189"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..2273)
                     /gene="fnbB"
                     /locus_tag="NBI42_002542"
     CDS             complement(<1..2273)
                     /gene="fnbB"
                     /locus_tag="NBI42_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HCD1778927.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATERKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPTTVQAP
                     KVETSRVDLPSEKVADKKTKGTQVDTAQPSNVSEIKPRMKRSTDVAAVAEKEVVEKEV
                     KATGTDVTGKVEVTESSLEGHNKDSNIVNPHNAERVTLKYKWKFGEGIKKGDYFDFTL
                     SNNVETHGISTLRKVPEIKSSTEDKVMANGQVIDERTIRYTFTDYIDNKKDLMAELNL
                     NLFIDPTTVTKHGDQNVEVKLGRNTVSKTFNIKYLDGVKDRMGVTVNGRIDTLNKTDE
                     KFSHFAYVKPNNQSLSSVTVTGQVTSGYKQNTNNPTVKVYKHIGSDELAESVYAKLDD
                     SSKFEDVTAELTLHYTSNGGYSLNFNNLDQTKDYVIKYEGDYDSNTNALEFQTHLFGH
                     YNYYYYNTSNLTWKNGVAFYSNNAQGDGKDKPKEPIIEHSTPIELEFKSEQPVEKHEL
                     TGTIEESNDSKPIDFEYHTAVEGVEGHAEGTIETEEDTIHVDFEESTHENSKHHADVV
                     EYEEDTNPGGGQDTTESNLVEFEEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVD
                     ELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYE
                     GGQNSGNQSFEEDTEEDKPKYKQGGNIVDIDFDSVPQIHSQNKGDQSFEEDTEKDKPK
                     YEHGGNIIDIDFDSVPQ"
BASE COUNT          824 a          555 c          411 g         1196 t
ORIGIN      
        1 atttgtggca cactgtcgaa gtcgatatca atgatattac cgccatgttc atatttaggc
       61 ttgtctttct ctgtatcttc ttcgaatgac tgatcacctt tattttgact atgaatttga
      121 ggtacactat cgaaatcgat atctacgata ttgccacctt gtttatattt aggtttatct
      181 tcttctgtgt cttcctcgaa tgactggtta ccgctatttt ggccaccttc ataacctaat
      241 tcactcttaa tatcaacgtg gctattttct tcgatttctt caatcacgcc ataattaccg
      301 tgaccatttt cagttcctaa accagaatga gaaatatgat gattgttttc agtaatctct
      361 tcgattggac cttgagcttg accatgttct tcaggtaatt catccactag ttcgataaga
      421 ttactttcag ttgtatattc tttcgtatct tcaattgttg tatggtcgct cactgcgcca
      481 gttacaatac cttttgtaga atcttcttca aattcaacta gattagactc agtagtatcc
      541 tgaccaccac ctgggtttgt atcctcttca tattcaacaa catcagcgtg atgttttgaa
      601 ttttcatgag tagattcttc aaaatccaca tgaatagtat cttcttcagt ttcaatagta
      661 ccttctgcat gaccttctac accttcaaca gctgtatgat attcaaaatc aattggctta
      721 gaatcgttac tttcttcgat tgtaccagtc aattcatgct tctccactgg ttgctctgat
      781 ttaaattcaa gttcgatagg agtactatgt tctataatag gttcctttgg tttatcttta
      841 ccgtcgcctt gagcgttgtt agagtaaaat gcaacgccat ttttccatgt taaattactt
      901 gtattataat agtaatagtt ataatgacca aaaagatgcg tttgaaactc taatgcgtta
      961 gtattcgaat catagtcacc ttcatacttt attacataat cttttgtttg atctaagtta
     1021 ttaaaattta aagaataacc accattactt gtataatgta aagttagttc cgcagtcaca
     1081 tcttcaaatt tgctggaatc atcaagcttt gcataaacac tttcagctaa ttcatctgaa
     1141 ccgatgtgtt tatatacttt aactgttgga ttattagtat tttgtttata tccagatgtt
     1201 acttgaccag ttactgtcac agagcttaac gactggttgt taggtttcac gtatgcaaaa
     1261 tggctaaatt tttcatctgt tttattcaag gtatcaattc gaccattaac agtaacaccc
     1321 attctatctt taacaccgtc taagtattta atattaaatg ttttactaac tgtatttctc
     1381 cccaatttga cttcaacatt ttggtcccca tgctttgtca ccgttgttgg atcgataaat
     1441 aagtttaaat ttaattcagc cattaaatct tttttgttat ctatataatc agtaaatgta
     1501 tagcgaattg ttcgttcatc tataacttgg ccatttgcca taactttatc ttctgttgaa
     1561 ctttttatct ccggaacttt acgcagtgtt gagataccat gtgtttcaac attattactt
     1621 aacgtgaaat caaaataatc tcctttctta attccttcac cgaatttcca tttatatttt
     1681 aaagttactc tttcagcgtt atgaggatta acaatattcg aatctttatt atgtccttct
     1741 aaagagcttt cagtaacttc cactttacct gttacatctg tacctgtcgc cttaacttct
     1801 ttctccacta cttctttctc tgcaactgct gcaacgtcag ttgatctttt cattcttggt
     1861 ttaatttctg agacgttact tggttgagct gtgtcaactt gagttccttt agttttctta
     1921 tcagcaactt tttccgatgg caaatcaact cgcgaagttt ctacttttgg tgcttgcaca
     1981 gttgttggtg cttcttctgt tgttacttgt gttgattgtg atggttgctc agttgatgtc
     2041 gcgctgtatg attgtgtttc atctattgta ttaacgttat ttgtagttgt ttgtgtttcg
     2101 cttgcttttc tttcagtagc tgaactccca ctttcctcta ctgtagtatt gttttgttcc
     2161 gatgctgcag cttctttttc ttgtcccatt ccaacaacga tcattgttcc taagaatact
     2221 gaggccgctc ccaatttgtg ttttcttatg ccgtatctaa gattgctttt cactataata
     2281 ttctccctta aatgtaaaat tcatttattt tttaaactca ataaatgcaa ttctatattg
     2341 ttcggttttt aaaagcaatg aaaaaaagcg agttaataaa aagttaagat tatcattaac
     2401 tttatcaaaa ttggtatttt tattatttag aatgagcaat taaattgtat acaaacagct
     2461 taaaaccctt gatattattg gcttgtattt tttatactta ctttttaaat taaattcaat
     2521 ttcatctaat ttaaaacaat atactaaaac ctacataata gtcgcctgta caatgcatca
     2581 ttaacaagtc actgaaacgc ctttcattgt attaataacg tcactatttt tttatatcgt
     2641 tcggtttttg tttgatttta atgattattt atacagaaac agccgtattt caagacgaca
     2701 ttttaaattt aactaaattt gcatctagtt aataattgca tttatcaaat ttgtcttatt
     2761 gattcaatct aatttgtact tacaaactag ttttaaattc taactttatc tctcagttcg
     2821 ttatcaatca tcagacataa accaatgaag caatcagaaa acactctaat tttctatgta
     2881 gaaatttgat ttaatctaaa aaaacaggct tacttcatat aatttatgaa ataaacccgt
     2941 caattttaga ttgattatgc tttgtgattc tttttatttc tgcgta
//
LOCUS       DAHHRR010000186         2926 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.190, whole genome shotgun sequence.
ACCESSION   DAHHRR010000186 DAHHRR010000000
VERSION     DAHHRR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.190"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..539
                     /locus_tag="NBI42_002543"
     CDS             <1..539
                     /locus_tag="NBI42_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HCD1778928.1"
                     /translation="KDTALKQSTSENSSLSKLEDVQVKDGDNSKNKGPVYVDVKGAVK
                     HPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIG
                     VNSVHVKNGNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKK
                     VKGFGSKTFDKLKSYFTT"
     gene            631..1092
                     /locus_tag="NBI42_002544"
     CDS             631..1092
                     /locus_tag="NBI42_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCD1778929.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            1097..>2926
                     /locus_tag="NBI42_002545"
     CDS             1097..>2926
                     /locus_tag="NBI42_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HCD1778930.1"
                     /translation="MLYVALSMIVGVLWNSSKVLSTFLFILLLYIAYRKNKIVYAPIS
                     LFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTNKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIIVQSCLESNWSNLIEK
                     HKQFIMNRIYNSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLLITKQIKI
                     KGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIIFIISVYYIFCLLAKRKYILVTFC
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVMDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTL
                     MLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKN"
BASE COUNT         1028 a          385 c          471 g         1042 t
ORIGIN      
        1 ataaagatac tgctctgaaa caaagcacta gtgaaaatag tagtttgtcc aaattagaag
       61 atgtccaggt caaagatgga gataattcca aaaataaagg tcctgtatat gtcgatgtaa
      121 aaggtgctgt taaacatcct aatgtttata aaatgacatc taaggataga gtagttgatt
      181 tgcttgataa agcacaatta ttggatgatg cagatgtaag tcaaattaat ttgtctgaaa
      241 aattaacaga tcaaaaaatg attttcattc ctcataaagg acaaaagaat gttgaaccac
      301 aaattggagt aaacagtgtg cacgtaaaaa atgggaacac gaataatact aaagtaaatt
      361 taaatacggc atctgtatca gaattgatgt ctgttcctgg agttgggcaa gctaaagcta
      421 atgcaattgt tgaatatcgc aaccaacaag gtgcatttca agaaattgac gatttgaaaa
      481 aagtaaaagg ttttggaagt aaaacttttg ataaactgaa atcttatttc acgacataat
      541 ggattatttt aattgcaaat tttatttttt gtgattaatc ttatattatt agaaaatgtt
      601 actaaatttt aagtatggaa ggtgaacatc ttggaaagaa tcaaatggga agaatatttt
      661 atggcacaaa gtcatttgct agcattacgt tcaacttgtc aaagactatc tgtaggtgca
      721 acgattgtta aggataatcg tattattgct ggtggttata atggctctgt agctggcgag
      781 gtgcattgta tagatgaagg atgtttaatt gaagatggac attgtatcag aacgatacat
      841 gcagaaatga atgctttatt acaatgtgca aaacaaggtg tatctactga aggtgcaaca
      901 atctatgtta ctcattttcc atgcctaaat tgtacaaagt caattattca agcaggtata
      961 aagcgtatct actatgcaga agattatcat aaccatgaat atgcaactaa attactcaaa
     1021 caatctggta ttgaatttaa aaaaattcca ttttcaccag aatatgttgc taaatatctg
     1081 actaaaggtt aatgttttgc tgtatgtagc gttatcaatg attgtaggag tactttggaa
     1141 ttctagcaaa gtgctctcta catttctttt cattttactt ttgtatattg cttatcgtaa
     1201 aaataaaatc gtttatgccc ctatttctct ctttttaatc attttctcct catggtattt
     1261 acattattca caacaagcaa tatttaatta tatcaattat attgaacgta attctcagtt
     1321 taatgagcgt gctcaagtaa tccaaattca acgtcaaggt agtgacacat ataaaggtag
     1381 gttgagttta aaaaatgaaa tatatccttt ctttttaaca aataaaaaga attttgattt
     1441 aaagaaaatt gaaagtcgta attgtattgt taaaggacaa ttcaaagtta atgacaataa
     1501 gtttgtaact cttaaattac aaagtataat tgtacaaagc tgcctagaat cgaactggtc
     1561 taatttaatt gagaaacata aacagtttat aatgaatcga atttataatt cgggtattaa
     1621 gtttccggat cgtattatgg cattgattac tggtgacgta aaagaaatta atgagcaatt
     1681 taaggaacgt gttaaagaga taggtatata tcatttgctg gcagttagtg gctcgcatat
     1741 agctgcaatt gtattcttaa tttaccaacc tttaaaacga ttaaatttac ctttatttgt
     1801 cattaaagga attacaatca ttgtattagc tttatttgct caatacacaa attatgcacc
     1861 tagtgctgta agagctataa taatgacaac tcttgtactg cttattacta agcaaattaa
     1921 aataaagggt attcagctat tagcatttgc atttataatt atgtttattt taaatccgct
     1981 agttgtttat gatattggat ttcaattttc atttatcatt tcatttttta ttatgctact
     2041 tttccctttt ttacagcaat tgtcaaagtt acaatcatta ttcataatta cgtttattgc
     2101 acaattagct tcatttatcg ttgccattcc aaactttcat caacttcaat gggtgggatt
     2161 tttatctaat ttgatttttg taccgtacta ttcgattata ttgtttccgc tatctatttt
     2221 attctttatt acaagtcatt ttattgtggg attaacgccg ctaaattact tggttgacct
     2281 aagttttaat tttcatgact ggttactaga cctattcaca agaatcaagc aatcacattt
     2341 ttccgttccc aagtttaatg attggatatt tataatattt ataatttctg tttattacat
     2401 attttgttta ttggctaaac gtaaatatat attggttacg ttttgtacta taattattct
     2461 gacattatta ataacgtttc caacaaattc acatcacaaa attacaatgt taaatgtggg
     2521 gcagggagac agtattttat atgaaggtgg taagaaccaa aatgtcttga ttgatacagg
     2581 tgggaaagtg atggatgata ctaaacaacc tagttattca atttctaaat atcatatttt
     2641 accaacgcta aatgaaagag ggataaatga attagagtat ctaattttaa cacatccaca
     2701 caatgaccat attggtgaag tggaatatat tattagtcat attaaaatta aacatatagt
     2761 gatatacaat aagggatata gtagtaatac attgatgtta ttatcgaaat taagccataa
     2821 gtataacatt aaacttatgg atgtaagaca agttagtagc tttaaacttg gagatagtag
     2881 ttttctattt tttgatagtt ttattccaaa tagccgagat aaaaat
//
LOCUS       DAHHRR010000187         2785 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.191, whole genome shotgun sequence.
ACCESSION   DAHHRR010000187 DAHHRR010000000
VERSION     DAHHRR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.191"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(8..655)
                     /locus_tag="NBI42_002546"
     CDS             complement(8..655)
                     /locus_tag="NBI42_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1778931.1"
                     /translation="MKFENYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSAQNITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQHAGVPSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            complement(768..1316)
                     /gene="hxlB"
                     /locus_tag="NBI42_002547"
     CDS             complement(768..1316)
                     /gene="hxlB"
                     /locus_tag="NBI42_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HCD1778932.1"
                     /translation="MATFSNYQLILDELTTTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEHGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVSEQTMQQNHANLE"
     gene            complement(1318..1950)
                     /gene="hxlA"
                     /locus_tag="NBI42_002548"
     CDS             complement(1318..1950)
                     /gene="hxlA"
                     /locus_tag="NBI42_002548"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HCD1778933.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKTIVAENPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(2027..2785)
                     /gene="nagB"
                     /locus_tag="NBI42_002549"
     CDS             complement(2027..2785)
                     /gene="nagB"
                     /locus_tag="NBI42_002549"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCD1778934.1"
                     /translation="MKVLNLGSKEQASFYVACELYKEMAFNQECTLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDHVTTFNLDEYVGLIASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASRYNDLLGQQGQRDIQILGIGENGHIGFNEPGTLFDSVTHIVDLTK
                     STIKANSRYFENEDDVPKQAISMGLANIFQAKRIILLAFGEKKRAAITYLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
BASE COUNT          818 a          539 c          407 g         1021 t
ORIGIN      
        1 acaaatttta taatactgta ttaatttctg atggatcatt gattataaaa tctggatttg
       61 aatgaagtaa tgaccttgca tcatgtgctc cccatgttac tgctgcagat ggtacaccag
      121 catgttgcgc catctcaaca tcaaatgttg aatcaccaat ataaaccgtt tgttggctat
      181 ttaaattgta gcgttgcaca attgtgtgta tgccttcagg atttggttta tatgcactta
      241 cttgatcgga tccaacagct tcggtaatca agtggtttaa tccaatagcc gataaatttc
      301 tttctaatac atcactcttc ttactagaca ccacgaaaag ttttttccct tggttataca
      361 aacttgtaat ggcttcagtt atacccgcaa attcataaat atagtccttt tcaatagatt
      421 gatatgtatg tctaaatgta tcgattaact tcgctaatgc tgcttcatct aatggtcggt
      481 ctgctaattt taaaaatgat tcttcaatag gtattcccat ataatgcgtt atgttttgag
      541 ctgatggttc tgttaagcca catgctttaa atgcactttg tgttgctact tcaccacatt
      601 ttttcgtgtc tgccaacgta ccatcaaaat caaaaatata attttcaaac ttcactatta
      661 acacccctat tcgtttatta ttgatattat actataattt attatttaaa taaaaaaata
      721 atcgataata tttctgccta ggcatcatat tatcgactat ctttgtttta ttctaaatta
      781 gcatgatttt gttgcatcgt ttgttctgaa acattcattt cagtcatcaa tcccattaca
      841 acgctatcta aaaatagttg agatgcttgt tcaaacaaac ttcctaacgg ttgtgcagaa
      901 ccatgttcat catattttgt acctgcaggc aaaacgatgt tcgtcccagc tagattgcct
      961 attgcagaat ctttatttgt agtaattaat acgatgtcag cacctactga ttttgcttta
     1021 tctgctaata atcttaaatg ttccgtggaa cctgaaccag agataattac aaatacatca
     1081 ttcgacttaa tcgcaggtgt cgttgattct ccaacaacat gtgcctgttt gccgagctga
     1141 tttaagcgca ttgcaaagct attcgctacg aatcctgaac gtcctttgcc agctacaaat
     1201 atatgttcag catgtagtat tttggacgca aacgttgaaa actcatccgc ttcaacatga
     1261 gaaagtgttg tcgttaattc atcaagaatt aattgataat tactaaaagt agccatatta
     1321 tttaccttct atagccgctc gacattgttt tgctgcttca actggatcgt ctgcatttgc
     1381 aattccgcca ccaacaatta caaggtctgg attttcagca acaatcgttt taattgtatc
     1441 tggtttaatt ccacctgcta ctgcaacttt agaattttta ataacagatt taacggttct
     1501 taaactttct aatggtgatt gcccttctgc ttgtaaatca taaccagtgt gtactgcaat
     1561 ataatcagca cccatttcat ctagttcttt tgcacgtttt tctaaatctt gaacagcaat
     1621 catatcaact agtaattgtt tattattttt atgagcttct tcaatagctg ctttaattga
     1681 tgcatcttct gcaacaccta gtattgtaat tacatccgcg ccaaatttaa ttgcttggct
     1741 aacttcataa tcagctgcat ccataatttt catgtctgct aatactttta cattactaat
     1801 gttgtctgcc atatgtttta ctgctggtaa accttcgttg taaatgattg gcgtaccgat
     1861 ttctacgata tctacataat cttttacttt atttgctaac tcagccgcgt cttctttgtt
     1921 taataaatca attgctaatt gtaattccac taaaaatcat ccttccaatg ctttttatct
     1981 ttctattgga acctttcccg aggcatttct taattaaaca ttgtaatcaa tccatttcat
     2041 cgacatgaat tttcgcaaca tttttcgggc aagcttcgtc gtctaaatat atctcaacat
     2101 tcgggtgttt gtgaagtaat gtggctggaa catcaacaga aatttcctga tttaataaat
     2161 atgtaatagc agcacgtttc ttttcaccaa atgcgagtaa aatgatacgt ttagcttgaa
     2221 aaatattagc aagtcccatc gaaatggctt gctttggaac atcatcttcg ttttcaaaat
     2281 atcgactatt agccttaata gtacttttag tcaaatcaac gatatgagta acgctatcaa
     2341 acagcgtacc aggttcatta aatccaatat gaccattttc tccaatacct aaaatttgaa
     2401 tatcacgttg accttgttgt cctaaaaggt cattgtatct tgacgcttca gcattcatat
     2461 catcggcatc tccattcgga atatgaatgt tctttctatt aaaataagga tattgtttga
     2521 aaagcatgtc atccatataa tagtgataac tttgcggatg tgatgcgatt aaacctacat
     2581 attcgtctaa attaaatgta gttacatgat ctacattgag ttgattttta tttaatagtt
     2641 ttacaagttg ttcatacaaa tctgtcattg ttccacccgt tgctaatccg agtgtacatt
     2701 cttgattaaa agccatttct ttatataatt cacatgcaac gtagaatgat gcttgttctt
     2761 ttgatcctaa gtttaatact ttcat
//
LOCUS       DAHHRR010000188         2702 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.192, whole genome shotgun sequence.
ACCESSION   DAHHRR010000188 DAHHRR010000000
VERSION     DAHHRR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.192"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            10..552
                     /locus_tag="NBI42_002550"
     CDS             10..552
                     /locus_tag="NBI42_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HCD1778935.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENINEKGAMPTTIKELNERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISSAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            706..972
                     /locus_tag="NBI42_002551"
     CDS             706..972
                     /locus_tag="NBI42_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCD1778936.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            975..2693
                     /gene="ptsP"
                     /locus_tag="NBI42_002552"
     CDS             975..2693
                     /gene="ptsP"
                     /locus_tag="NBI42_002552"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCD1778937.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMDGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
BASE COUNT          982 a          373 c          528 g          819 t
ORIGIN      
        1 gataatatta tgggattcaa aaataattta acgtcaaatt taactaataa aattggtaat
       61 tcagttttta aaatagaaaa cattaatgaa aagggtgcaa tgcctacgac aattaaagaa
      121 ttaaatgaaa gacgacaacg tgctgaagca attgtaaaga gaaagtcttt aatgtcatca
      181 acaatgagcg ttgttccaat tcctggatta gactttggtg ttgatttaaa attaatgaaa
      241 gatattatcg aagatgttaa taaaatttat ggtttagatc ataagcaagt taatagcctt
      301 ggagatgatg tgaaagaaag aattatgtct gcagcagcaa ttcaaggtag tcaatttatt
      361 ggtaaaagaa tttcaagtgc atttttaaaa atagttatta gagatgtagc taaacgtact
      421 gctgcaaaac aaacgaaatg gttcccggta gtaggacaag ctgtatctgc atcaattagt
      481 tattatttta tgaataaaat tggaaaagat cacattcaaa aatgcgaaaa tgttattaaa
      541 aatgtcatgt aggtgctata atagttttgc aatttgcaaa ttttactgaa accggtttta
      601 aacgaattga atttaaagca tggttttggt aaagttaatg tataaaacta agttagtatt
      661 gtaataatat tgaagattct aactatacga aggagaaatg taattatgga acaaaattca
      721 tatgtaatca tcgacgagac tggtattcac gctagaccag caacaatgtt agtacaaaca
      781 gcttcaaaat tcgattctga tattcaatta gaatataacg gtaagaaagt taacttaaaa
      841 tcaatcatgg gtgttatgag ccttggtgtt ggtaaagatg ctgaaattac aatttatgct
      901 gacggtagtg atgaatctga cgccattcaa gcaatcagtg acgtcttatc aaaagaagga
      961 ttgactaaat aatcatgtct aaattaatta aaggtattgc cgcatctgat ggtgtcgcaa
     1021 ttgctaaagc ttatttatta gttgagccag acttaacatt cgacaaaaat gaaaaagtca
     1081 ctgatgttga aggagaagtt gcaaagttca atagcgcaat cgaagcttct aaagttgagt
     1141 taactaaaat tagaaataat gcagaggttc aactaggtgc tgataaagct gctatctttg
     1201 atgcacattt attagtttta gatgaccctg aattaattca accaatccaa gataagatta
     1261 aaaatgaaaa cgctaatgct gctacagcat taacggatgt aacaacacaa tttgttacaa
     1321 tttttgaatc tatggataac gaatacatga aagaacgtgc ggctgatatt cgcgacgttt
     1381 ctaaacgtgt gttatcacat attttaggtg tagaattacc gaatccgagt atgattgatg
     1441 aaagcgttgt tattgtaggg aatgacttaa cgccatctga tactgctcaa ttaaataaag
     1501 aattcgtaca aggttttgct acaaacattg gcggaagaac aagtcactct gcgattatga
     1561 gtcgttcttt agaaattcca gcaattgttg gtacaaaatc aattactcaa gaagttaaac
     1621 aaggcgacat gattatcgta gatggattaa atggtgatgt aatcgttaat ccaactgaag
     1681 atgagttaat cgcttatcaa gataaacgtg agcgttattt tgctgacaag aaagaattac
     1741 aaaaactacg tgatgctgat actgttacag ttgatggtgt tcacgcagag cttgctgcaa
     1801 atattggtac acctaatgat ttgccaggtg ttattgaaaa tggtgcacaa ggtatcggct
     1861 tatatagaac tgagttttta tatatgggtc gtgaccaaat gcctacagaa gaagagcaat
     1921 ttgaagctta taaagaagta ttagaagcaa tggacggtaa acgtgttgtt gtacgtactt
     1981 tagatatagg tggagataaa gaattatcat acttaaactt gcctgaagaa atgaatccat
     2041 tcttaggtta tcgtgcgatt cgcttatgcc ttgcgcaaca agatattttc agaccacaac
     2101 tacgtgcatt attacgtgca tcggtttatg gtaagttaaa tatcatgttc ccaatggttg
     2161 caacaattaa cgaatttaga gaagctaaag ctatattatt agaagaaaaa gaaaacctta
     2221 aaaatgaagg tcatgacatt tcagatgata tagaattagg aatcatggta gaaatacctg
     2281 caacagcagc attagctgat gtctttgcta aagaagtaga tttcttcagt atcggtacaa
     2341 atgatttaat tcaatacaca ttagctgctg accgtatgtc agagcgtgta tcatacctat
     2401 accaaccata taacccttca atcttacgtt tagttaaaca agttattgaa gcgtcacata
     2461 aagaaggtaa atggacaggt atgtgtggtg aaatggctgg agatgaaaca gctattccat
     2521 tattgcttgg tttaggttta gatgagttct ctatgagtgc aacgtctatt ctgaaagcaa
     2581 gaagacaaat taatggttta agtaaaaatg aaatgactga acttgctaac cgtgcagtcg
     2641 actgtgcaac gcaagaagaa gttattgaat tagttaacaa ctacgtaaaa taattaattc
     2701 tt
//
LOCUS       DAHHRR010000189         2692 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.193, whole genome shotgun sequence.
ACCESSION   DAHHRR010000189 DAHHRR010000000
VERSION     DAHHRR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.193"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            154..256
                     /locus_tag="NBI42_002553"
                     /pseudo
     CDS             154..256
                     /locus_tag="NBI42_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            240..>2692
                     /locus_tag="NBI42_002554"
     CDS             240..>2692
                     /locus_tag="NBI42_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HCD1778938.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKVSETQTTTANVNTIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANLETVKEEVVKEEAKPQVETTQPQDNSGNQRQVDLTPKKATQNQVTETHVEV
                     AQPRKASESKPRVTRSADVVEAKDAKVDTGTDVTSKVTVTESSIEGHNNTNKVEPHAG
                     QRAVLKYKLKFEDGLKKGDYFDFTLSNNVNTYGVSTARKLPEIKNGSVVMATGQLLGD
                     GKIRYTFTDYIDYKVNVTANLNLNLFIDPRIVKNNGEETLTSKINGKNTEKKIEVEYK
                     DGVGKYYTNLNGSIETFNKADNKFTHVAYVKPINGNKSESVSITGSLTQGSNVSGDSP
                     IVKVYEYQGKETDLPKSVSVNLTDNSKFKDVTSDMQNKLTVQENGNYQLNLEKLDKTY
                     VIHYTGEYSKETDEVNFRTQVSAYPENSYRYYSYYNNHYTLTWDNGLVLYSNKADGNG
                     QNGVKVESNNLIFDEITGTGVIKGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGEGGYV
                     DGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHAD
                     VVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIEL
                     VDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELG
                     YEGGQNIGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGDQSFEEDTEKDK
                     PKYEHGGNIIDIDFDSVPH"
BASE COUNT         1091 a          378 c          533 g          690 t
ORIGIN      
        1 aatttaaaaa ataagtataa aaaatacaag ccttgtgtga caagggtttg tgatgacttg
       61 aatacaattt ataggtatat ttcaaataat aaaattgtca attaacataa aattaatgac
      121 aatcttaact tttcattaac tcgctttttt gtattgcttt taaaaaccga acaatataga
      181 cttgcattta ttaagtttaa aaaaattaat gaattttgca tttaaaggga gatattatag
      241 tgaaaaacaa tcttaggtac ggcattagaa aacataaatt gggagcagca tcagtattct
      301 taggaacaat gatcgttgtt gggatgggac aagataaaga agctgcagca tcagaacaaa
      361 agacaactac agtagaagaa aatgggaatt cagctactga taataaagta agcgaaacac
      421 aaacaactac agctaacgtt aatactatag aagaaactca atcatataac gcaacagtaa
      481 cagaacaacc gtcaaacgca acacaagtaa caactgaaga agcaccaaaa gcagtacaag
      541 caccacaaac tgcacaacca gcaaatttag aaacagttaa agaagaggta gttaaggaag
      601 aagcgaaacc tcaagttgaa acaacacaac ctcaagacaa tagcggaaat caaagacaag
      661 tagatttaac acctaaaaag gctacacaaa atcaagtgac agaaacacac gttgaagtgg
      721 cacagccaag aaaggcatca gaaagtaagc cacgtgtgac aagatcagca gacgttgtgg
      781 aagctaagga tgcgaaagtg gatacgggta cagatgtgac gagtaaagtt acagttactg
      841 aaagttctat cgagggtcat aacaatacaa ataaagtaga acctcatgca ggacaaagag
      901 cggtgctaaa atataaattg aaattcgaag atggactaaa aaaaggagat tactttgatt
      961 ttacattatc aaataatgta aatacctatg gcgtatcaac agcgagaaag ttaccagaga
     1021 ttaaaaatgg ctctgtcgta atggctactg gtcaacttct tggagatgga aaaattagat
     1081 acacgtttac agattacatt gattataaag tgaatgtaac agcaaattta aacttgaatt
     1141 tatttataga ccctagaatc gttaaaaata atggtgaaga aacacttact tcaaaaataa
     1201 atgggaaaaa tactgaaaaa aagattgaag ttgagtataa agatggagtt ggaaagtatt
     1261 atacaaacct gaatgggtca attgaaacat ttaataaagc ggataataaa ttcacacatg
     1321 tagcttacgt taaaccgata aacggaaata aatctgaaag tgtatctatt actggtagtt
     1381 tgacacaagg tagtaatgta agtggtgatt cacccattgt taaagtgtat gagtatcaag
     1441 gtaaggaaac ggatttacca aagagtgttt ctgtaaactt aacagacaat tcgaagttta
     1501 aagatgttac aagtgatatg caaaataaat taactgtaca agaaaacgga aattatcagt
     1561 tgaatctaga aaaattagat aagacatatg tgattcatta cacaggagag tactcaaaag
     1621 aaacagatga agttaacttt agaactcaag tatctgcata tccggagaat agttacagat
     1681 actattctta ttataataat cactatacat taacttggga taatggttta gttttatata
     1741 gtaataaagc agatggtaat ggtcaaaatg gtgttaaagt agaatcgaat aatttgattt
     1801 ttgatgagat aacaggaaca ggagttatta aaggtcaata tgataagaat ttagtaacta
     1861 ctgttgaaga agaatatgat tcttcaactc ttgacattga ttaccacacg gctatagatg
     1921 gtgaaggtgg ttatgttgat ggatacattg aaacaataga agaaacggat tcatcagcta
     1981 ttgatatcga ttaccatact gctgtggata gcgaagcggg tcacgttgga ggatacactg
     2041 agtcctctga ggaatcaaat ccaattgact ttgaagaatc gacacatgaa aattcaaaac
     2101 atcacgctga tgttgttgaa tatgaagagg atacaaaccc aggtggtgga caggttacta
     2161 ctgagtctaa cttagttgaa tttgacgaag agtctacaaa aggtattgta actggcgcag
     2221 tgagcgacca tacaacagtt gaagatacga aagaatatac aactgaaagt aatctgattg
     2281 aactagtaga tgaactacct gaagaacatg gtcaagcaca aggaccaatc gaggaaatta
     2341 ctgaaaacaa tcatcatatt tctcattctg gtttaggaac tgaaaatggt cacggtaatt
     2401 atggcgtgat tgaagaaatc gaagaaaata gccatgttga tattaagagt gaattaggtt
     2461 acgaaggtgg ccaaaatatt ggcaatcaat catttgaaga agacacagaa gaagacaagc
     2521 ctaaatatga acaaggtggc aatatcgtag atatcgattt cgatagtgta cctcaaattc
     2581 atggtcaaaa taaaggtgat cagtcattcg aagaagatac agagaaagac aagcctaaat
     2641 atgaacatgg cggtaatatc attgatatcg atttcgacag tgtgccacat at
//
LOCUS       DAHHRR010000190         2682 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.194, whole genome shotgun sequence.
ACCESSION   DAHHRR010000190 DAHHRR010000000
VERSION     DAHHRR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.194"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(221..832)
                     /locus_tag="NBI42_002555"
     CDS             complement(221..832)
                     /locus_tag="NBI42_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCD1778939.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYRVADRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTDDIKNVTFELVKSVNDIEQV"
     gene            1040..1753
                     /locus_tag="NBI42_002556"
                     /pseudo
     CDS             1040..1753
                     /locus_tag="NBI42_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270135.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1792..>2682
                     /locus_tag="NBI42_002557"
     CDS             1792..>2682
                     /locus_tag="NBI42_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCD1778940.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHITLDIEHSLKCLVLKLITENKQEDGY
                     KIIDEFLCIDKSYNNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPAKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQSIIAN
                     LNYDSRSPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQNRYKRNESYYKNALAIKRF"
BASE COUNT         1025 a          397 c          394 g          866 t
ORIGIN      
        1 tattatggaa ttgaaagaag ttaattttaa ggttgtcgaa tgtaaaaaca tattgattaa
       61 ccataaaaga gaaattattt ttgtagattt cggcataaac gtcattaaag aattttctat
      121 atgatggtgc taatcataac aaagaaagct aggcaccttt agatacctaa ctttcaaagt
      181 attagcgata aaacactata tatactaatt taacttaatt tcaaacttgt tcaatgtcat
      241 taacactttt cacaagttcg aaagttacat tttttatatc atcagtatta atttttacat
      301 tttccctatc tttttgtagc tctttatgcg actctaatgt atatttacca ccatcctttg
      361 tatttatgac gatgttacct ttaggcaaat gttctcccat atataagcca taagaaatat
      421 gagcaaatct tatgatatga tccaattctt ttaaagtgac aacttcttta ttaagagtaa
      481 tgttcctctt aggtgtatac actctatcag caactctgta tgttccttct aaatgtatac
      541 gaacttggtt ttcatttaaa ggtctctctg aataaaccca attcctagat ttattagatt
      601 ttccatttat tgtatccttg ctgtatctat cgtaaagttt tttaaccaaa gctttttctg
      661 aattatttgg tacttcttca actttttgta attgtttatc ttgtacactt gaggaatttt
      721 gtgttgacgc gtctgctttt ggatatattc caaatgttcc cccatagatg atacctagcg
      781 atagaataga ttttaatacg attgaatttt ttgagtcata ttttttgaac atatttaatt
      841 acctccttga agtaaaactt tatttgctac aattataaaa ataatagacg tgttcatgaa
      901 ttaaattcat cttaactctt gattaacttt agtttgctac cactctcaaa tttaataact
      961 ataagacgcc tacacttaat tggacaaaat ctatgagaat agaatttgtt aatttaaaaa
     1021 aagtaggcaa ttcaaagtta taagtgtaat agataaaata gatatcccta tagtgatgcg
     1081 ttactagcta aacgtaataa cactttagaa gataatgtag tgaaagaaat accggattgt
     1141 ttcgactatg taattaagta taaaaatatc caacgagaaa acgtaattat aaaatggtaa
     1201 aagctatggt acagtttcaa attgctaatg gcatgcgtat cggtgagcta cttgcaataa
     1261 agagagaaaa tataaactat gaagataaaa cgctagatat cgatggtaca attaattgga
     1321 taactaaaaa aacacgggag cattcggagt aaaggagaca actagaacaa gtaacagcta
     1381 tagggctata ggcctcacta tccatagcat cgacatacta agaacactta tgcttggaat
     1441 gataagttta ttaatatagg ttacatattc acaaatgcag ctggtggccc tatcgactcg
     1501 aacaaaatta gcaacattat taaaggtggc actatcaaaa agacaactga gattagttct
     1561 attaagaaac ctgtaacgac gcatacatta catcattcgc atatatctac acttgctcaa
     1621 ttagtaatta acttaaaagc aaagcaagag cgtgtaggtc attcagatta taaaaaatct
     1681 agagatatac acacatgtta ctaatcagat ggcggaagat atgatgaata aatttgaacg
     1741 attggggagt taatattgaa aaaagatgat acactagcag aaattaagcc tatgctcaat
     1801 tttgatgagc aaatagcgaa attaaaacag atgaatatat tttttaatat tattgacacc
     1861 gaaaaagcaa atgaaattct tagaaaaaat aattacttct tcaaactagc ttatttccga
     1921 aaaaatttcg aaaaaaagaa tggcggctat ttcatagaat ttgcttattt atcagattta
     1981 gcaactatag atatgaaatt aagatacaca atgttgcata taactttaga tattgaacat
     2041 agtttaaagt gtctagtctt aaaactaata acagaaaata agcaagaaga tggttataaa
     2101 ataatagacg agttcttatg tattgataaa tcatataaca attcaaattt tgacacaaat
     2161 tcaagaacac cagaagaagt tatggaaacc aaaatcaaaa ataaaaacga aatattcaag
     2221 catatgaata aaagagggca actacccgcg aagttgaata aatactatca aaatccaccc
     2281 gcatgggttt gcattgaatt catgcaacta ggtcaattcg tttcgtttct caacttctat
     2341 tacaagaagt acaatgacga agaattgaga gttgctaata ttttaatgcc tttagttaaa
     2401 aatataagaa acaaatcagc tcataaccaa tccatcatag caaatctaaa ttatgacagt
     2461 agatctcctc aatatttatt tgaaaaaggg aataatatag gcatatctag aaacatgttc
     2521 ggaataaaaa atttcataga tactttcgct acgctagaat tgcataatca agtttgtagt
     2581 aatgcaatta tccaagcaag atatcacgat ttggaccaac ttcaaaatcg atataaaaga
     2641 aacgaaagct attataaaaa tgcattagct atcaaaagat tt
//
LOCUS       DAHHRR010000191         2653 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.195, whole genome shotgun sequence.
ACCESSION   DAHHRR010000191 DAHHRR010000000
VERSION     DAHHRR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.195"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            278..1579
                     /locus_tag="NBI42_002558"
     CDS             278..1579
                     /locus_tag="NBI42_002558"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCD1778941.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIDGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGCAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARTLLIEAIDSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            1593..1943
                     /locus_tag="NBI42_002559"
     CDS             1593..1943
                     /locus_tag="NBI42_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778942.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKKPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(2095..2565)
                     /locus_tag="NBI42_002560"
     CDS             complement(2095..2565)
                     /locus_tag="NBI42_002560"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCD1778943.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
BASE COUNT          904 a          390 c          509 g          850 t
ORIGIN      
        1 ttataaaaaa tatctaagca tgggacatcg taaccatata taagtatgtc gatatcaact
       61 ttaagacatc ataaatgcca tgatgattct aagataattt aatagggact ttgacgttat
      121 aaccaatcta tttaagaata ataacatcgc attgaaattg tgatattaaa aatgaaatcg
      181 atagcttgct ataataattt tcgcttgtgc ttttaaaaat ggactagaga aagcgcagat
      241 gctttgtcct aagcttttta agaaaaggat gataaaaatg agaatgatag acattattga
      301 gaaaaagcgt gacggtcata cactaacaac ggaagaaatt aatttcttta ttgacggcta
      361 tgttaaaggg gatattcctg attaccaagc atcaagttta gcaatggcga tttatttcca
      421 agatatgaat gatgatgagc gtgcagcatt aacgatggct atggttaatt ctggtgatat
      481 gatagatttg agtgatatta aaggtgtcaa agtagataag cactcaacag gtggtgtagg
      541 agatacaact acattggttt tagcaccatt agtagctgct gtagatgttc ctgttgcaaa
      601 aatgagtggg cgtggattag gtcatacagg tggtacgatt gataaattag aagcaattga
      661 tggttttcat gttgaaatag atgaagcaac atttgtgaaa ttggttaatg aaaataaagt
      721 ggcagttgta ggacaatcag gaaatttaac tcctgcagat aaaaaattat atgccttaag
      781 ggatgttact ggtactgtca attcaatacc attaattgcc tcttcaatta tgagtaaaaa
      841 gattgccgct ggtgcagatg caattgtatt agatgtaaaa actggtagcg gtgcatttat
      901 gaaaacatta gaagatgctg aagcattagc gcacgcaatg gtacgtattg gtaataatgt
      961 gggacgtaat acgatggcga ttatttctga tatgaatcag ccacttggtt gcgcgattgg
     1021 caatgcgctt gagttacaag aagcaattga tacgttgaaa ggacaaggtc caaaagattt
     1081 aacagaactt gtattaacat taggttctca aatggttgta cttgcaaaca aagctgaaac
     1141 gcttgaagaa gcgagaacgt tgctaattga ggcaattgat tcaggtgcag cattagaaaa
     1201 attcaaaaca tttattaaaa accaaggtgg cgacgaaaca gttattgacc atccagagcg
     1261 tttgccacaa gctcaatatc aaatcgaata taaagctaaa aaatcaggtt atgtgactga
     1321 gttagtctct aacgatatag gtgtcgcttc aatgatgtta ggagcgggac gtttaacaaa
     1381 agaggatgat attgatttag cggttggtat tgttttaaat aaaaaaattg gggataaagt
     1441 agaagaagga gaatcattat taacgattca cagtaatcgc caagatgtag acgatgttgt
     1501 taaaaaatta gattcaagca taacaattgc tgatcatgtt gtatcgccta cattaattca
     1561 taaaattatt acagagtagg agttgaagct gattgaacat actaagatat ttattaggta
     1621 taggttttag tgtaataggt gtattgcatt ttacacgaga acgacaattt agaaatatca
     1681 taccgaaatg tttgccgctt cgaaaaacag cggttcttgt tacaggaatc tttgagatat
     1741 tatttgggct tgcactttta attaaaaaac catctcaatg cttgaaaaat acaatcaatc
     1801 tattcttgct agctgtattc ccagctaata tctatatggc agttaaaaag ttacctttag
     1861 gagataaaca attgccaaca tggcaactat atttaaggtt acctatgcag ttcgtgttaa
     1921 tgggactaat taagaaatta tagtggttct taaataaatc ggacaaatgt taaagatttt
     1981 actttttcat ttgttcgatt ttttgacttt gaagcataat aaaaagcagt tggacaatgg
     2041 cgattgtaac atcattgttc gactgctttt ctgattaact tgattgacta agctttattt
     2101 tcctgtaccg aaaacatcat gccattcgtt tcgtttgtct agaaatgctt gagcgatagt
     2161 tttagcacct tctaatgaat gactagctgc ccagccacat tgtacttcgt tacaagctgg
     2221 cacttcagta gcatttagca catcatttaa agttgcttca acaatattta atacatcatc
     2281 ataattatca tgattaataa atgatacata gaaaccagtt tggcaaccca taggacttaa
     2341 atcaacgact ttgtcactat gatttctaat attttcagcc attaaatgtt ctaatgaatg
     2401 caatccgggc atatccatat gttctttgtt tggttgtttg aaacgaatgt catatttgtg
     2461 aatgacatct ccgtttaatc cttccatcgt tcccgctaat ctaataaatg gggcaaccac
     2521 tttagtatga tctaaattga aactttcaac attcattttt gtcattttaa atttcctcct
     2581 attaactact caaaactata aaacgcgaat gatttggtac attattaaac ttaaatttgt
     2641 tgcaagttgc gtt
//
LOCUS       DAHHRR010000192         2633 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.196, whole genome shotgun sequence.
ACCESSION   DAHHRR010000192 DAHHRR010000000
VERSION     DAHHRR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.196"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..1481
                     /locus_tag="NBI42_002561"
     CDS             <1..1481
                     /locus_tag="NBI42_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HCD1778944.1"
                     /translation="GFRAYVFAPVIKGKVDKSQLLIGYAGTDPLSLNDIHTDLQLPIH
                     HNTNNLKINNHYKDLKNSTIHKKYNTIKDKNDLFSFSSNFNNKVLFGELWAAKFAPTQ
                     MDESDAFTESVKKKYPDSEIHPLGHSLGAFLAQYNLIKHNLDNGTTFAAPNVYHSFTG
                     DFKKDIDNGVYDSKIRNIGHFNDPINNFNFFDHPIGENITVMPHYDGFITALPLIGQH
                     TLESYNDFDADGTVKEMPELEQFNHDPFLNNRFQLFDPFGINSRFFDSFKIDSSILNR
                     PSENDFDPFAILNKKDKLFESNNLDVERFNNKHKDDLSIDLNPLDDELNDDDPLIPKR
                     GDSKKLKAEKESSVKKGKGGSKNTGKKVKIQPDEVRNIANTIRDRLFLYNQITNALDE
                     YKEETISGANRILDKYQSELLSGSHEFITPYDLEQYMEILAVEGSSGNFKFYNSGLMD
                     DLIQELDQNKKDLLQFAEKLEFAANKFEEKDLEESDVFGLFS"
     gene            1496..1795
                     /locus_tag="NBI42_002562"
     CDS             1496..1795
                     /locus_tag="NBI42_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HCD1778945.1"
                     /translation="MSSKDAKKSQEEARLQQLKQEMRNRTDNMVSNVKNNVALKSQTL
                     NEIIETINNSSQNLEGTFEGKASEAAQQSINKLKNNNENLKQKFETLLNTMKING"
     gene            complement(2048..>2633)
                     /locus_tag="NBI42_002563"
     CDS             complement(2048..>2633)
                     /locus_tag="NBI42_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HCD1778946.1"
                     /translation="NYKVKAYLSLLNLKGKNLYLIDDIKISELEDELVDNYDLHQNNL
                     IVGDTINRLDLVSFSNEQLYEEIKSLKDRLINKRTTLMTSIYNQYLETILNGDFTNRT
                     KLKEAYIGMKDIDTIEIIYNNLNKVSNELKSSNEKIINLSAFIRDNHCNNNDNIQLEY
                     IIGEMKKLKDGTKDRVTQMCFSLLIDTLKGIIDE"
BASE COUNT         1007 a          370 c          345 g          911 t
ORIGIN      
        1 atgggtttag agcatatgtt ttcgctccag taatcaaagg caaagttgat aagagtcaac
       61 ttttaatagg ttatgctggc actgacccac taagtctaaa cgatattcat actgacttac
      121 aacttcccat tcatcataac acaaataatc taaaaattaa taatcattat aaagatttaa
      181 aaaactcaac tattcacaaa aaatacaata caattaaaga taaaaatgac ctttttagtt
      241 tttcttctaa ttttaataat aaagtacttt tcggtgagct atgggcagct aagtttgcac
      301 cgactcaaat ggacgaaagt gatgctttta ctgaatcagt taaaaagaaa tatccagata
      361 gtgaaattca cccattagga catagtttag gtgcgttttt agctcaatat aatttaataa
      421 aacataatct ggataacggt acaacgtttg cagcacctaa cgtataccat tcatttactg
      481 gagactttaa aaaagacatt gataatggtg tctatgactc aaaaatacga aatattggcc
      541 attttaatga tcctattaat aactttaatt tcttcgatca tccaattggt gaaaatataa
      601 ccgtaatgcc acattatgat ggatttataa ctgctcttcc tttaattggt caacacacac
      661 ttgaatctta caatgatttc gatgcggatg gtactgtgaa ggaaatgcca gaacttgaac
      721 aatttaatca tgacccattt cttaataata gatttcagtt atttgaccca tttggtataa
      781 atagcagatt tttcgactct ttcaaaatcg atagttctat attaaataga ccaagtgaaa
      841 atgattttga tccttttgct attctcaata agaaagataa attattcgaa tcaaataatc
      901 ttgatgtaga acgatttaat aataaacata aagatgattt aagcattgat ttaaatcctt
      961 tagatgatga actaaatgat gatgacccat taattcctaa aagaggtgat agtaaaaaat
     1021 taaaagcaga aaaagaaagc tctgtaaaaa aaggtaaagg tggttccaaa aatacaggta
     1081 aaaaagtaaa aatccagcct gatgaagtgc gtaatatcgc caacacaatt cgtgatagac
     1141 tatttttata taatcaaatc actaatgcat tggatgaata taaagaagaa acaatatcag
     1201 gggctaatcg cattttagat aaatatcaat cggagcttct aagcggttca catgaattta
     1261 taacacctta cgatcttgaa caatatatgg agattttagc agttgaggga agttcaggaa
     1321 atttcaagtt ttataatagt ggtttaatgg atgacttaat tcaggaatta gatcaaaaca
     1381 aaaaagactt gctacaattt gctgaaaaac tagaatttgc tgcaaataaa ttcgaagaaa
     1441 aagatttaga agaaagcgat gtttttggac tatttagtta aagagggtaa tttatatgag
     1501 ctcaaaagat gcaaagaaga gtcaagaaga agctagatta caacaattaa agcaagaaat
     1561 gcgtaatcgt actgataata tggttagcaa tgttaaaaat aatgtagcat taaaatctca
     1621 aacattaaat gaaatcattg aaacaattaa taattcttcg caaaatttag aaggtacatt
     1681 tgaaggaaaa gcaagcgaag cagcccaaca aagtataaat aaactcaaaa acaataatga
     1741 aaatcttaaa caaaaatttg agaccctatt aaatacaatg aagataaatg gatgatattg
     1801 agaaattatt aatttatctg tactttttat acattaagaa aatcccctcg ctaaacaaat
     1861 agtgaaggga ttttaattct tatttctgat ctaacttatt ttagtatcat taataaaaac
     1921 tacaatttta tcatactaca gattcaccaa tttaaatgta taatttaatt atattcttat
     1981 taagtcacta tttaaaaaga agcatactct ttatatattt atcctattaa acaaattaat
     2041 atgtctatta ttcatctatt atacctttca gtgtatcgat caacaagcta aaacacattt
     2101 gtgtaactct atctttagta ccatctttta attttttcat ctctcctata atatattcta
     2161 attgaatatt atcattatta ttacaatgat tatctctaat gaatgcactt aaattaataa
     2221 ttttctcatt actactttta agctcatttg atactttgtt taaattatta tagattattt
     2281 ctatagtatc tatatctttc attccaatat acgcttcttt taatttagtt ctatttgtaa
     2341 agtcaccatt taaaatagtt tctaagtatt ggttatatat agaagtcatt aaagttgttc
     2401 ttttatttat taaacggtct tttaaacttt tgatttcttc atatagttgt tcgtttgaaa
     2461 aactaactaa atctagtcta tttatcgtat ctccaactat taaattattc tgatgcaaat
     2521 cgtaattatc cactaattca tcttctaatt cgctaatttt aatgtcgtct attaaatata
     2581 agttttttcc ttttaaattt aataaagata aataagcttt tactttataa tta
//
LOCUS       DAHHRR010000193         2595 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.197, whole genome shotgun sequence.
ACCESSION   DAHHRR010000193 DAHHRR010000000
VERSION     DAHHRR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.197"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(244..858)
                     /locus_tag="NBI42_002564"
     CDS             complement(244..858)
                     /locus_tag="NBI42_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCD1778947.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGVFCIILSTLLLRNKLIN"
     gene            complement(875..1945)
                     /locus_tag="NBI42_002565"
     CDS             complement(875..1945)
                     /locus_tag="NBI42_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCD1778948.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHSGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDNQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            complement(1957..2466)
                     /locus_tag="NBI42_002566"
     CDS             complement(1957..2466)
                     /locus_tag="NBI42_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1778949.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
BASE COUNT          833 a          476 c          334 g          952 t
ORIGIN      
        1 tattcaaaaa aagttttagt ttctaattga caatgattct cactaatgta taatagtagt
       61 tgaaaatgat tatcaatacc acatagaaca tcccccccac aacgtttcgt tcttgttgga
      121 ttggtcattt tcaaatattc cccttttata tgcccgtaaa agacaatata cgttataaca
      181 acgttttata aaagcagtaa acccttacga cactttaggt ttactgcttt tgttttttac
      241 tatttaattg attaatttat ttctaagtaa taacgttgat aaaataatac aaaatacgcc
      301 accaataatg ccggcaataa tatctgttgg ataatgtaca cctagatata cacgtgatat
      361 ggaaatcaat aaaatcatag ctgcacataa ccctataaga ataccttttg aattaccttg
      421 atttaatcga tttaatagat agataccact tccaaaatat gcagttgatc ccatagcatg
      481 accgctagga aagctaaatc ctgttatatc aattaaacgc agcaatgtag gcctttctct
      541 atcgaatata ttttttaatg ctggattcaa aattccagat aatgccattg ttaatgcaaa
      601 aaataatgct tcaattttgt ggcgctttaa catgagatat gccacaagaa gtaatgaaat
      661 acataacatt gcccagactt cacctacttt agtagcccca agcatgatag atgtcgtaat
      721 aaagctctct gatgaatata caaattcata aacttcatta tcaatccatt tccctagtct
      781 tgattcgtgg aaaaacgcga taattccaaa aaccaatgta aaaacgatga gcaaagagat
      841 acgtttccat tgactcataa agcattaccc cccattaatt caatgcgtct ttaatcatct
      901 tatcaaataa agcttctttc gtataacttt gttcatacga tttcatatta ttgataattc
      961 gagttctttc ctgttccatt tcatttagtt cttgtaataa aatttgtgct gttaattgtt
     1021 cttcatcaat cgctttagca taacctttat cagcaaaatg atttgcattg tcaatttggt
     1081 cgcctcggga ttgatctaaa cctaatggta ctaataacat tggtatacgt aatgttaaga
     1141 actcatatat cgcatttgat cctgctctac ttattactgt gtccgtaatt gctaataaat
     1201 ctgttaaatc ctctttaaca aattcatatt gtatatatcc tgatttttta acttgattat
     1261 cttttaatcc tttaccagtt aaatgtatca cttgatattg ttgtaataat gcatctaagt
     1321 tttcgcgaat aatgctattt aattttttac ttcctaagct tccacccata acgagtaaaa
     1381 cttttttatt ttcattaaag cctgttaatt gataaccact atgtgcatta ccatttttta
     1441 aatcttctcg aattgttgct ccaataaaat cagctttctc tttaggtaga tagtttagcg
     1501 tttcttcaaa tgttgtatat attttcttgg caaatttaag tgctatctta ttcgctaatc
     1561 ctggtgttaa gtcagattca tgaataatag ttggtatatt taatgatttg gctgcaataa
     1621 caacaggcac agatacaaat ccaccttttg aaaatagtag atcaggtttt tcttttttca
     1681 aaactttacg agcatcaaga atacctttca atactttaaa tacatctttg gcattttcta
     1741 aagaaatata tcttcttaat ttaccactcg aaataggata atacttaatt tctggtagtt
     1801 gtgattcaat catttctctt tcaataccat ttttagaacc aatataaagc gcttcataac
     1861 cttgtgataa tgcagttgga attaaactta aatttactga tacgtgtcca actgttcccc
     1921 ctccggtaaa tgcgattttc gtcattaata ttacctctat tcttttaaat ttttataata
     1981 tgcataaaat ggttcacctt tatcatatgg cggatattcc attaattgtt caccgacctt
     2041 atgaaatcca aatttggcaa ataaaccttg tgcaggtttg tttaacgcaa aggtgtccgt
     2101 taaaataact tctgcaccac gtgctttaac tacatcaata acataattga ataattctgt
     2161 agcagctcct ttatattctt tcgaaccagt taatcgatga ataacaaagg caccttctct
     2221 atttactggc cagtcaatgt catcatacca ttctgcttgg tcttggtcga caacaataaa
     2281 gccataaatt ttgtcatttt cctctaatac gtacaaataa tctttagcaa tatcttcttc
     2341 aaaatgttct aaaagagggt actgatcgtc ccattgctcg ttgtcgtgtt ctttcattaa
     2401 ttcttttgct tcttctacta gatttaagat ttgatctaaa tctgacattt tacctagacg
     2461 gatcatgtac gtcactcctt atatcgttat gttaaacatg tttttcagta catgttaatc
     2521 ttacacacta ttaacatgct ttaaaccttt attatgaatg aaaaatagta attatcacac
     2581 tatgggataa aaata
//
LOCUS       DAHHRR010000194         2570 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.198, whole genome shotgun sequence.
ACCESSION   DAHHRR010000194 DAHHRR010000000
VERSION     DAHHRR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.198"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(129..212)
                     /locus_tag="NBI42_002567"
     tRNA            complement(129..212)
                     /locus_tag="NBI42_002567"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(176..178),aa:Leu,seq:caa)
     gene            complement(251..325)
                     /locus_tag="NBI42_002568"
     tRNA            complement(251..325)
                     /locus_tag="NBI42_002568"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(290..292),aa:Gly,seq:tcc)
     gene            complement(429..503)
                     /locus_tag="NBI42_002569"
     tRNA            complement(429..503)
                     /locus_tag="NBI42_002569"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(468..470),aa:Gly,seq:tcc)
     gene            complement(511..584)
                     /locus_tag="NBI42_002570"
     tRNA            complement(511..584)
                     /locus_tag="NBI42_002570"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(550..552),aa:Cys,seq:gca)
     gene            complement(590..661)
                     /locus_tag="NBI42_002571"
     tRNA            complement(590..661)
                     /locus_tag="NBI42_002571"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(627..629),aa:Gln,seq:ttg)
     gene            complement(673..745)
                     /locus_tag="NBI42_002572"
     tRNA            complement(673..745)
                     /locus_tag="NBI42_002572"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(710..712),aa:His,seq:gtg)
     gene            complement(748..821)
                     /locus_tag="NBI42_002573"
     tRNA            complement(748..821)
                     /locus_tag="NBI42_002573"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(787..789),aa:Trp,seq:cca)
     gene            complement(837..917)
                     /locus_tag="NBI42_002574"
     tRNA            complement(837..917)
                     /locus_tag="NBI42_002574"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(881..883),aa:Tyr,seq:gta)
     gene            complement(923..998)
                     /locus_tag="NBI42_002575"
     tRNA            complement(923..998)
                     /locus_tag="NBI42_002575"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(963..965),aa:Thr,seq:tgt)
     gene            complement(1006..1078)
                     /locus_tag="NBI42_002576"
     tRNA            complement(1006..1078)
                     /locus_tag="NBI42_002576"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1043..1045),aa:Phe,seq:gaa)
     gene            complement(1097..1172)
                     /locus_tag="NBI42_002577"
     tRNA            complement(1097..1172)
                     /locus_tag="NBI42_002577"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1137..1139),aa:Asp,seq:gtc)
     gene            complement(1182..1255)
                     /locus_tag="NBI42_002578"
     tRNA            complement(1182..1255)
                     /locus_tag="NBI42_002578"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1219..1221),aa:Met,seq:cat)
     gene            complement(1270..1362)
                     /locus_tag="NBI42_002579"
     tRNA            complement(1270..1362)
                     /locus_tag="NBI42_002579"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1324..1326),aa:Ser,seq:tga)
     gene            complement(1401..1476)
                     /locus_tag="NBI42_002580"
     tRNA            complement(1401..1476)
                     /locus_tag="NBI42_002580"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1441..1443),aa:Asp,seq:gtc)
     gene            complement(1487..1576)
                     /locus_tag="NBI42_002581"
     tRNA            complement(1487..1576)
                     /locus_tag="NBI42_002581"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1538..1540),aa:Ser,seq:tga)
     gene            complement(1586..1659)
                     /locus_tag="NBI42_002582"
     tRNA            complement(1586..1659)
                     /locus_tag="NBI42_002582"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1623..1625),aa:Ile,seq:cat)
     gene            complement(1688..1761)
                     /locus_tag="NBI42_002583"
     tRNA            complement(1688..1761)
                     /locus_tag="NBI42_002583"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1725..1727),aa:Met,seq:cat)
     gene            complement(1783..1858)
                     /locus_tag="NBI42_002584"
     tRNA            complement(1783..1858)
                     /locus_tag="NBI42_002584"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1823..1825),aa:Ala,seq:tgc)
     gene            complement(1876..1949)
                     /locus_tag="NBI42_002585"
     tRNA            complement(1876..1949)
                     /locus_tag="NBI42_002585"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1913..1915),aa:Pro,seq:tgg)
     gene            complement(1959..2032)
                     /locus_tag="NBI42_002586"
     tRNA            complement(1959..2032)
                     /locus_tag="NBI42_002586"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1996..1998),aa:Arg,seq:acg)
     gene            complement(2051..2139)
                     /locus_tag="NBI42_002587"
     tRNA            complement(2051..2139)
                     /locus_tag="NBI42_002587"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2103..2105),aa:Leu,seq:taa)
     gene            complement(2150..2224)
                     /locus_tag="NBI42_002588"
     tRNA            complement(2150..2224)
                     /locus_tag="NBI42_002588"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2190..2192),aa:Gly,seq:gcc)
     gene            complement(2228..2309)
                     /locus_tag="NBI42_002589"
     tRNA            complement(2228..2309)
                     /locus_tag="NBI42_002589"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2273..2275),aa:Leu,seq:tag)
     gene            complement(2313..2388)
                     /locus_tag="NBI42_002590"
     tRNA            complement(2313..2388)
                     /locus_tag="NBI42_002590"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2353..2355),aa:Lys,seq:ttt)
     gene            complement(2394..2469)
                     /locus_tag="NBI42_002591"
     tRNA            complement(2394..2469)
                     /locus_tag="NBI42_002591"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2434..2436),aa:Thr,seq:tgt)
     gene            complement(2486..2561)
                     /locus_tag="NBI42_002592"
     tRNA            complement(2486..2561)
                     /locus_tag="NBI42_002592"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2526..2528),aa:Val,seq:tac)
BASE COUNT          717 a          656 c          606 g          591 t
ORIGIN      
        1 acacagagcc tattaaagcg tgtgccattt gagtgccact tttatttgta ttgtataaag
       61 aaaaataaaa agaaaccttg ttttacaagg tttctagtac gttatgttat gtaaataaca
      121 gttaattata ccggtggtcg gggtcgaacc gacactccac aagtggaacg ggattttgag
      181 tcccgcgcgt ctgccaattc cgccacaccg gcttatgggt aaacaaaaaa cttccctttg
      241 gaagcaatta tggagcggaa gataggattt acacctatac ctagttccgg gaaggaacgt
      301 gttctaaaag ttgaactact cccgcaaata ttaaattatg gagcggaaga taggatttac
      361 acctatacct cattccagga aggaatgtat tctaagagtt gaaatactcc cgcattatta
      421 ttaaattatg gagcggaaga taggatttac acctatacct cgttccggga aggaacgtgt
      481 tctaaaagtt gaactactcc cgcataaacc tggaggcggc aaccggattt gaaccggtga
      541 taaaggtttt gcagacctct gccttaccac ttggctatgc cgccaataac tgggctagct
      601 ggattcgaac caacgagtga cggagtcaaa gtccgttgcc ttaccgcttg gctatagccc
      661 attaataata agggcggctg aaggggatcg aaccctcgaa tgtcggaacc acaatccgat
      721 gtgttaacca cttcaccaca gccgccatgg caggggcagt aggaatcgaa cccacaccaa
      781 aggttttgga gacctctatt ctaccgttga actatgcccc tattaaaaat aataaatgga
      841 ggggggcaga ttcgaactgc cgaacccgaa ggagcggatt tacagtccgc cgcgtttagc
      901 cacttcgcta cccctccata aatggtgccg gccagaggac ttgaaccccc aacctactga
      961 ttacaagtca gttgctctac caattgagct aggccggcta agaaatggtt caggacagag
     1021 tcgaactgcc gacacatgga gcttcaatcc attgctctac caactgagct actgaaccat
     1081 aataaaaatg taatgatggc ggtctcgacg ggaatcgaac ccgcgatctc ctgcgtgaca
     1141 ggcaggcgtg ttaaccgcta cactacgaga cctataaaat attgcgggag gcggatttga
     1201 accaccgacc ttcgggttat gagcccgacg agctaccgaa ctgctccatc ccgcgataat
     1261 aaaaaataat ggcggaggaa gagggattcg aacccccgcg gcccgttaag gccctgtcgg
     1321 ttttcaagac cgatcccttc agccggactt gggtattcct ccattattat aggtaaatcg
     1381 ctattaatta taaaattaaa tggcggtctc gacgggaatc gaacccgcga tctcctgcgt
     1441 gacaggcagg cgtgttaacc gctacactac gagaccatta gtaaaacgga ggaagaggga
     1501 ttcgaacccc cgcgagccgt taagcccctg tcggttttca agaccgatcc cttcagccgg
     1561 acttgggtat tcctccaaaa ttatatggac cttgcaggac tcgaacctgc gaccgaacgg
     1621 ttatgagccg ttagctctaa ccaactgagc taaaggtcct aaatataatt ttacaactaa
     1681 taaatagtgg cggtggaggg gatcgaaccc ccgacctcac gggtatgaac cgtacgctct
     1741 agccagctga gctacaccgc cttatatagt ttgtaaataa tatggtggag actagcggga
     1801 tcgaaccgct gacctcctgc gtgcaaagca ggcgctctcc cagctgagct aagcccccat
     1861 aataattaca gtatatcggg aagacaggat tcgaacctgc gaccccttgg tcccaaacca
     1921 agtgctctac caagctgagc tacttcccgt ataattaacg cgcccgatag gagtcgaacc
     1981 cataacctct tgatccgtag tcaaacgctc tatccaattg agctacgggc gcatatgttt
     2041 ttattgaaaa tggtgccgag gaccggaatc gaaccggtac ggtgatcact caccgcagga
     2101 ttttaagtcc tgtgcgtctg ccagttccgc caccccggca ctataaaaat ggagcagaag
     2161 acgggattcg aacccgcgac cccaaccttg gcaaggttgt attctaccgc tgaactactt
     2221 ctgcatatgc gggtgaaggg agtcgaaccc ccacgccgta aggcgctaga tcctaagtct
     2281 agtgcgtctg ccaattccgc cacacccgca aatggtgagc catagaggat tcgaacctct
     2341 gaccctctga ttaaaagtca gatgctctac caactgagct aatggctctt ccatggtgcc
     2401 ggccagagga cttgaacccc caacctactg attacaagtc agttgctcta ccaattgagc
     2461 taggccggca atatgtaaga ataaatggtg gagaatgacg ggttcgaacc gccgaccctc
     2521 tgcttgtaag gcagatgctc tcccagctga gctaattctc cgatttaaaa
//
LOCUS       DAHHRR010000195         2527 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.199, whole genome shotgun sequence.
ACCESSION   DAHHRR010000195 DAHHRR010000000
VERSION     DAHHRR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.199"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            164..841
                     /locus_tag="NBI42_002593"
     CDS             164..841
                     /locus_tag="NBI42_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HCD1778950.1"
                     /translation="MIGTATSQFPHSPISMSSEAKAYNISESETNVSELTKYYTQRHL
                     TFSNKWLWQKDNGTIHATLLQLSWFSHIQVFGPESWGNINQLRNKYVDIFALKDYETW
                     RTYMLAQETFTGGVTPAAKPSDKHYKLNLTYKDKSGTFIGEYPFYTGNKPVLTLKEVD
                     FRIRQTLIKNKKLYHGNYNKGHIKITGGSNNYTIDLNKRLKSTDANRYVKNPQTAHIE
                     ILLEKSN"
     gene            950..1666
                     /locus_tag="NBI42_002594"
     CDS             950..1666
                     /locus_tag="NBI42_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HCD1778951.1"
                     /translation="MRKSITNKIILTTTLLLLGTTATQTPNSPLNVSTDAKAYYIDQD
                     ETNINELIKYYTQKPLTFSNRWLYQYDDGNIYVEFKRYSWSAHIRLWGAESWGNINQL
                     RDHYVDVFGLKDEDTRQLWWVYRDTFTGGVTPAASSSDKPYSLFVQYKDKLQTIIGAH
                     KMYQGNKPILTLKEIDFRAREALIKNKILYHENRNKGKLKITGGGNDYTIDLSKRLHS
                     DLANVYVKNPQKITVEVLID"
     gene            complement(2066..2266)
                     /locus_tag="NBI42_002595"
     CDS             complement(2066..2266)
                     /locus_tag="NBI42_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778952.1"
                     /translation="MTELNEIINAIKSLFESESGYKISKNSGVPYQTVQDLRNGKTKI
                     EDARFRTIIKLYSYYTSLKEQS"
     gene            complement(2250..2396)
                     /locus_tag="NBI42_002596"
     CDS             complement(2250..2396)
                     /locus_tag="NBI42_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778953.1"
                     /translation="MKVFYIAFLIILWLNIFLGNEIIHTLTVLITTLYIVNRRKRIKN
                     DRVE"
BASE COUNT          909 a          431 c          378 g          809 t
ORIGIN      
        1 tcgttagtat cattcattga gtgaatacaa atagactgaa tgatttaatt gatttctctc
       61 tttttataaa cttattattc ctcgcatata acattcctcg taaaggagat tttctaatga
      121 aaaacaacct cacaaaaaaa ttattttatc aatagcattg actatgatag gcacagctac
      181 aagtcaattc cctcattcac cgattagtat gtcatctgaa gcaaaagcat acaatataag
      241 tgaaagcgaa acgaatgtaa gtgaattgac taaatattac acacaacgcc atttaacttt
      301 ttcaaataaa tggctgtggc aaaaggataa tggtacaatc catgcaacat tgttacagtt
      361 atcatggttt agtcatattc aagtattcgg ccctgaaagt tggggtaata ttaatcaatt
      421 aagaaataaa tatgtagata tttttgcttt aaaagattat gaaacctggc gcacttatat
      481 gttagcccaa gaaacattta ctggtggtgt tacaccagca gcaaaaccta gcgataaaca
      541 ttataaattg aacctaacat ataaagataa atcaggaaca tttattggcg aatatccatt
      601 ttacacaggt aataagcccg tattaacttt aaaagaagtt gatttccgaa ttcgtcaaac
      661 attaattaaa aacaaaaaat tatatcatgg taattataat aaaggccaca ttaagattac
      721 aggtggcagc aacaactata caatcgattt aaacaaaaga ttaaaatcaa ctgatgcaaa
      781 tagatatgtt aaaaaccctc aaactgcaca catcgaaatc ctccttgaaa aatctaacta
      841 acatcgaaat cggagcaaac agaaataagc acaatggtta cattgacaca gttcattttt
      901 aaatttgtta attctcgtat tgatgaatac gaagaaagga gatgttcgaa tgagaaagag
      961 cattacgaac aaaataattt taacgacaac attgttatta ttaggaacaa cagctacaca
     1021 aacccctaat tcgccactaa atgtttctac tgacgctaaa gcttattata ttgaccaaga
     1081 cgaaacgaac atcaatgagt taattaaata ttacacacaa aagcctctca cattttcaaa
     1141 tagatggtta taccaatatg atgacggcaa catttatgtt gaatttaagc gttattcttg
     1201 gtcagctcat atacgattat ggggtgctga aagttggggc aacattaatc aattaagaga
     1261 ccactatgta gacgtatttg gtttgaaaga cgaagacact aggcagttat ggtgggtata
     1321 tcgtgataca tttacaggtg gtgtaacacc tgctgcatca tcttctgata aaccatatag
     1381 tctttttgtc caatacaaag ataaactaca aacaattatt ggtgcacata agatgtacca
     1441 aggtaataaa ccaatattaa cgttgaaaga aattgatttt cgagcacgag aagcgctaat
     1501 taaaaataaa atattatatc atgaaaatcg caataaaggg aaacttaaaa ttaccggtgg
     1561 cggcaatgac tatacaattg atttaagcaa aagattacat tctgatcttg caaatgttta
     1621 tgttaaaaat cctcaaaaaa taacagtcga ggtcctcatc gattaaatga tattgcatga
     1681 cttgtattgc aaattccaag gataaacaat acagtaatga tacttcgaca tgtatcaaca
     1741 atagatacac aataaaaaat tagtctacaa aatataaaat ggcatttgga gtcaatcaga
     1801 aatgccaaat cattctctgt gttggggccc ccccaacttg cattgcctgt agaatttcta
     1861 ttcgaaattc tctatgttgg ggcccacccc aactctcaca ttattgtaag ctgacttttc
     1921 gtcagcttct gtgttggggc cccgcccata attgaaaaat gtttgttaca tgggcatttt
     1981 tgtttggtca atgtctgtct atataatatt gtagagccta agtcgtggtt acgtcttagg
     2041 ctctactctt aatttaatga ttcaattatg actgctcttt taatgaagta taataactat
     2101 atagttttat aatcgttcta aatctagcat cttctatttt cgttttaccg ttcctcaaat
     2161 cttgtacggt ctgatacggc acccctgaat ttttcgatat tttgtagcct gattcagact
     2221 caaacaaaga tttaatcgcg ttgataattt cattcaactc tgtcattttt aatcctcttt
     2281 cttctattaa cgatatacaa tgttgttatt aaaacagtca gtgtatggat gatttcattt
     2341 cctaaaaata tattcagcca taaaataatt agaaatgcga tataaaatac tttcattata
     2401 atcccctatc atttttatat ttatcacttt ccacttagtt tcaacattaa aagctaactt
     2461 aggtttaaga ttctcttata acgcctataa ctataagtct gcattttatc ttgtatataa
     2521 tatttta
//
LOCUS       DAHHRR010000196         2405 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.200, whole genome shotgun sequence.
ACCESSION   DAHHRR010000196 DAHHRR010000000
VERSION     DAHHRR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.200"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            120..1160
                     /locus_tag="NBI42_002597"
     CDS             120..1160
                     /locus_tag="NBI42_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HCD1778954.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIMFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNNPYTRYPVYEGDIDNVIGVFHSKYLLAWSNKKEDQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            1174..2241
                     /locus_tag="NBI42_002598"
     CDS             1174..2241
                     /locus_tag="NBI42_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCD1778955.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPNESIIKRLKEANIKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLNPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELTHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
BASE COUNT          886 a          373 c          461 g          685 t
ORIGIN      
        1 ttaattatca ttattaagac tattatatat aatgaatttt aactggttta ttaaacgaga
       61 acgtcgggaa ttaagtaact acaataaaaa taagatatga caataaggag actacacgcg
      121 tgatcattgc cataattata ttgatattta tttcgttttt cttttcagga agcgagacgg
      181 cattaacggc tgccaataaa acaaaattta aaactgaagc tgacaaaggt gataaaaaag
      241 caaaaggcat tgtaaagtta cttgaaaaac caagtgagtt tattacaacg attctaattg
      301 ggaataatgt cgcgaatatt ttattaccaa cacttgttac aattatggct ttacgttggg
      361 ggataagcgt tggtattgcg tcagctgttt taacagttgt tatcattttg atttccgaag
      421 tgattcccaa gtctgtcgct gcaacatttc cagataaaat aacaaggctt gtatatccaa
      481 ttattaatat ttgtgtcatt atgttccgcc ctatcacatt acttttaaat aagttgacgg
      541 acagtattaa tcgaagttta tctaagggtc aacctcaaga acatcaattt tcaaaagaag
      601 aatttaaaac aatgttagca attgctggac atgaaggtgc tttaaatgaa attgagacga
      661 gtaggttgga aggtgtcatt aattttgaaa atttaaaagt aaaagatgta gatacaacac
      721 ctagaattaa tgtgacggca tttgcttcaa atgcgacata tgaagaagtt tatgaaacgg
      781 ttatgaataa cccatacact agatatccag tatacgaggg agatattgat aacgttattg
      841 gggtgtttca ttctaaatat ctgttggctt ggagtaataa aaaagaagat caaattacaa
      901 actattcagc taagccatta tttgtgaatg aacacaataa agctgaatgg gtattacgta
      961 agatgactat ttctagaaaa catttagcaa ttgtgttgga cgaatttggt ggtactgaag
     1021 cgatagtgtc acatgaagac ttaatcgaag aattattagg tatggaaatt gaagatgaga
     1081 tggataaaaa ggaaaaagaa aaactttctc aacagcaaat tcaatttcaa caacggaaaa
     1141 atcgcaacgt atctatataa ggagcgaaca gctatgtgga ataagaatcg acttactcaa
     1201 atgttaagta ttgaatatcc aattatacaa gcaggtatgg caggaagtac gacaccgaaa
     1261 ttagttgcat cagtaagtaa cagtggtgga ttaggcacaa taggcgcagg ttactttaat
     1321 acgcagcaat tagaagacga aatagattat gtacgccaat taacgtcaaa ttcttttggt
     1381 gtaaatgtct ttgtaccaag tcaacaatca tataccagta gtcaaattga aaatatgaat
     1441 gcatggttaa aaccttatcg acgcgcatta catttagaag agccggttgt aaaaattacc
     1501 gaagaacaac aatttaagtg tcatattgat acgataatta aaaagcaagt gcctgtatgt
     1561 tgttttactt ttggaattcc aaatgaatct attataaaaa gattgaaaga agcaaacatt
     1621 aagctaatag gtacagcaac aagtgttgat gaagctattg cgaatgaaaa agcgggtatg
     1681 gatgctatcg ttgctcaagg tagtgaagca ggtggacatc gtggttcatt tttaaatcct
     1741 aaaaatcaat tacctatggt tggaacaata tctttagtgc cacaaattgt agatgtcgtt
     1801 tcaattccgg tcattgccgc tggtggaatt atggacggta gaggagtttt ggcaagtatt
     1861 gttttaggtg cagaaggggt acaaatgggc accgcatttt taacatcaca agatagtaat
     1921 gcatcagaac tactgcgaga cgcaattata aatagtaaag aaacagatac agtcgttaca
     1981 aaagcgttta gtggaaagct tgcacgcggt atcaacaata ggtttatcga agaaatgtcc
     2041 caatacgaag gcgacatccc agattatcca atacaaaatg agctaacaag tagcataaga
     2101 aaagccgcag caaacatcgg cgacaaagag ttaacacata tgtggagtgg acaaagcccg
     2161 cgactagcaa caacgcatcc cgccaacacc atcatgtcca atataatcaa tcaaattaat
     2221 caaatcatgc aatataaata atcgaccgca atccacaaat gcacaagcac ccccaaacat
     2281 tattttagtg cttgccattt ttgtggattg cgtttctatt ttaccaatct agtcaaacga
     2341 aattatcaaa ctaaagttcg ctgaaatgat tataattaaa cgaaatcatc aaactaaagt
     2401 tcgct
//
LOCUS       DAHHRR010000197         2402 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.201, whole genome shotgun sequence.
ACCESSION   DAHHRR010000197 DAHHRR010000000
VERSION     DAHHRR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.201"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(471..1811)
                     /gene="glnA"
                     /locus_tag="NBI42_002599"
     CDS             complement(471..1811)
                     /gene="glnA"
                     /locus_tag="NBI42_002599"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCD1778956.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(1830..2198)
                     /locus_tag="NBI42_002600"
     CDS             complement(1830..2198)
                     /locus_tag="NBI42_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1778957.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
BASE COUNT          729 a          454 c          355 g          864 t
ORIGIN      
        1 gtagtataac taaaatgaaa ataccgatcg ataaacaaaa agatattttt tgttttgttt
       61 ctcttttcat atagtattac cccctttata atgagaggta agaaccccat ttcggggtct
      121 taccttagaa cgttctgcga atgaattcga tgagtagtag tatgaaagtc cctattttca
      181 aatagtactc aacattttcg cgacgttctt tttttattct tttatcgcct catctcatca
      241 cctccaaata tattaaaatt catgtgaact aaaatataga atggtcttcc ccagctttaa
      301 aaaaataaat acacaaaata cttacttgac caaaacttga ccaaaagttg cccaatcgta
      361 ataaaagaca cattttacac cttgaaaagt tgcccagtta aaccaaaaaa gagctaaagc
      421 aaaatgctct agcccttgat attactgatt tcccatcatt gtttagcgta ttaatattgc
      481 ttcatgtact gatctctttc ccattcagat acttgagttc tgtagtaatc ccattcaatt
      541 gattttgaat taataaattg attatagata tgatttccta aagctttttt aataacttca
      601 ttttcacgca ttgcttttaa tgcagtataa agtgttgaag gtaagtcttg aatgcctact
      661 gcttcacgtt cttcacggtt catttcgtaa atattttggt taactggttc tggaactttt
      721 aatttatttt taataccatc tagtccagct tctaagattg cagctaacgc catgtatggg
      781 tttgcagctg gatctactga acgtacttcg atacgagttg ataatcctct tgaagatggt
      841 acacggatta acggtgaacg gtttttaccg ctccatgcaa tataacatgg tgcttcataa
      901 ccaggaacta aacgtttgta tgagtttact aatgggttac ataccgctgt aaatccacgt
      961 gcgtttttaa gtacaccagc tgtgaattgg taagctgttt ctgttaagcc catttcagta
     1021 ttcggatcaa agaatgcatt ttctttacct ttgaataatg aaacgttaaa gtgcataccg
     1081 ctaccattca caccgaataa tggtttaggc ataaatgttg cgtgtaaatt atgtttacgt
     1141 gcgattgttt taacaaccaa tttaaatgtt tggatgttat cacatgctgt aacagcatct
     1201 gcatatttaa agtcaatttc atgttgacca ggggcaactt catggtgact agcttcaata
     1261 tcgaagccca tatcctctaa ttctagaaca atatcacgac ggcagttttc acctaaatca
     1321 gttggagcta agtcgaaata tccaccatca tcgttaagtt ctaaagttgg ttcccctttt
     1381 tcatccaact tgaacaagaa gaattctggt tcaggtccta ggttaaagtc tgtgaagcct
     1441 aaatcttcca tttcttttaa tacacgtttt aagtttgcac gaggatcccc ttcaaatggt
     1501 gtaccatctg ttttataaac atcacaaatt aaacgtgcaa ctttaccttg tccagcagtc
     1561 catgggaaga ttacccaagt atctaaatct ggatgtaagt acatatctga ttcttcgata
     1621 cgtacgaaac cttcgataga agaaccgtca aacatcattt cgttatcaag tactttttct
     1681 aattggctta caggcacttc aacattttta attgttccta aaatatcagt gaattgtaat
     1741 cttaaatatc ttacattttc ctcttctgca aatttacgaa tgtcgtcttt agtgaaagta
     1801 cgttttggca ttttaaaatc ctccaaattt tatttaataa atcgggataa atcaccacga
     1861 tttattggca aagtttctcc aataggcttt tgcgtggcat ctacaatcat cttttttctt
     1921 atctcttgtt catctgttgt taaatgctct tgtgagtcat aaatgatttg tttaatccct
     1981 ttgatattaa atcctttttc taataatgat ttaatttcta gtaacctttc caaatcattg
     2041 agtgagaaca gacgtttttg accttctgtt ctttcaggtt tgatgagttc atgtgtttca
     2101 tagtaacgta tttgccttgg cgttaaatcc gttaacttac ttactacact catagagaag
     2161 acagccatat ttcgtctgat tgcatcattc gatatcattg ttcctctcct ctacttttga
     2221 acttgttaat aacttatcat aattttttta ggatttcaaa aatatgtcag gttttctaac
     2281 atatagctca aaacattgat ataactgcaa tttcgacctc taaaacgcca taaaattacc
     2341 ttcaccaaaa atattttttc aatttttaaa aataatctga taaatcttcg taaaaaaatt
     2401 tc
//
LOCUS       DAHHRR010000198         2336 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.202, whole genome shotgun sequence.
ACCESSION   DAHHRR010000198 DAHHRR010000000
VERSION     DAHHRR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.202"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            165..1100
                     /gene="lukE"
                     /locus_tag="NBI42_002601"
     CDS             165..1100
                     /gene="lukE"
                     /locus_tag="NBI42_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HCD1778958.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIHESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLMTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPSGS
                     AREYFAPDNQLPPLIQSGFNPSFITTISHEKGTSETSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            1102..2085
                     /locus_tag="NBI42_002602"
     CDS             1102..2085
                     /locus_tag="NBI42_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukocidin/hemolysin toxin family protein"
                     /protein_id="HCD1778959.1"
                     /translation="MKMKKLVKSSVATSIALLMLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWIGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
BASE COUNT          913 a          322 c          393 g          708 t
ORIGIN      
        1 gttgcgccaa aatgaccggt tttcagcatc ataaatgaat aaattgtgtc atttttaata
       61 ttttatagca ttttattttt taatgaacag ttaattttat aaataagtga aataatctag
      121 aataaatttg tattaaaaat ttatatagaa agaaagtgaa acttatgttt aagaaaaaaa
      181 tgttagctgc aactttgtca gtaggactga ttgcaccttt agcatctccg attcacgaat
      241 ctagagcaaa tactaatatt gaaaatattg gtgatggtgc tgaagtaatc aaacgaacgg
      301 aggatgtaag tagtaagaaa tggggcgtta ctcaaaatgt tcaatttgac tttgtaaaag
      361 ataaaaaata taacaaagac gctttaattg ttaaaatgca aggttttatt aattccagaa
      421 cttcattttc agatgtgaag ggtagtggat atgagttaac aaaacgaatg atttggccat
      481 tccaatataa tataggactg atgactaaag atccaaatgt tagcttaatc aattaccttc
      541 ctaaaaacaa aatagaaact acggatgttg gtcaaacatt aggatataac attggaggta
      601 atttccagtc agcaccatct ataggtggca atggctcatt taattattct aaaacaatta
      661 gttataccca aaagagttat gtcagtgaag tagacaagca aaactcaaaa tctgttaaat
      721 ggggtgttaa agcaaacgaa tttgttacgc ctgatggtaa aaaatctgcg catgatagat
      781 atttattcgt acaaagtcca aatggtccgt caggttcagc aagagaatat tttgctcctg
      841 ataaccaatt acctccatta attcaaagtg gatttaaccc atcatttata acaacaatct
      901 cacatgagaa aggtacaagt gagacaagtg aatttgaaat ttcatatggt agaaacttag
      961 atattacata tgcgacttta ttcccaagaa ctggtattta cgcagaaaga aagcataatg
     1021 catttgtaaa cagaaacttt gtagttagat atgaagttaa ttggaaaaca cacgaaatta
     1081 aagtgaaagg acataattaa tatgaaaatg aaaaaattag tcaaatcatc agttgctaca
     1141 tcaattgctt tacttatgct atcgaataca gttgatgcag ctcaacatat cacacctgta
     1201 agcgagaaaa aagtagatga caaaatcact ttatacaaaa caacagcaac atctgataat
     1261 gataaattga atatttctca aattttaacg tttaatttca ttaaggataa aagttatgac
     1321 aaagatacgt tagtacttaa ggcagccgga aacattaatt caggttataa aaagcctaat
     1381 ccaaaagatt acaattactc acagttttat tggggcggta agtataatgt ttcggttagt
     1441 tcagaatcaa atgatgctgt aaatgttgtt gactatgcac ctaaaaatca aaatgaagaa
     1501 ttccaagttc aacaaacatt aggttattct tatggcggag atattaatat atctaatggc
     1561 ttatcaggtg gattaaatgg atcaaaatca ttttcagaaa cgataaatta taaacaagaa
     1621 agttacagaa ctacgattga tagaaaaaca aatcataaat caattggctg gggtgttgag
     1681 gcgcacaaaa ttatgaataa tggttgggga ccatatggta gagatagtta tgacccaaca
     1741 tatggtaatg aactgttttt aggtggtaga caaagtagtt caaatgctgg tcaaaatttc
     1801 ttgccaacac atcaaatgcc tttattggcg cgtggtaact ttaacccaga atttataagc
     1861 gtactttctc ataaacaaaa tgatacaaaa aaatctaaaa tcaaagtaac ttaccaaaga
     1921 gaaatggata gatatactaa tcaatggaat cgactacact ggattggtaa taactacaaa
     1981 aatcaaaata cagtaacgtt tacatctact tatgaagttg actggcaaaa ccatactgtt
     2041 aaattaatcg gtactgattc taaagaaact aatcctggag tatagaattg tacatgatta
     2101 aataatcaac aatgtgcaat tatttcaatt atcaaacgga attatagaac taacataatt
     2161 tttatttaca cacatgataa tttttagtta gggaaatgta gattgctttg ctaaatagca
     2221 gggtgctaaa tttgcgaaag tagaattatt tagaaacttc caacatataa aaaattatta
     2281 aaaaaatgga accacgtctt aattggcgtg gttctttttg tatatatagc atctat
//
LOCUS       DAHHRR010000199         2295 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.203, whole genome shotgun sequence.
ACCESSION   DAHHRR010000199 DAHHRR010000000
VERSION     DAHHRR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.203"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..300)
                     /gene="sarR"
                     /locus_tag="NBI42_002603"
     CDS             complement(<1..300)
                     /gene="sarR"
                     /locus_tag="NBI42_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HCD1778960.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKA"
     gene            complement(728..1054)
                     /locus_tag="NBI42_002604"
     CDS             complement(728..1054)
                     /locus_tag="NBI42_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCD1778961.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSDNVEMFVEYV
                     KQHMIV"
     gene            complement(1118..1860)
                     /locus_tag="NBI42_002605"
                     /pseudo
     CDS             complement(1118..1860)
                     /locus_tag="NBI42_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
     gene            complement(1886..>2295)
                     /locus_tag="NBI42_002606"
     CDS             complement(1886..>2295)
                     /locus_tag="NBI42_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1778962.1"
                     /translation="SKEINLTRLSIFIFIINILNRLRGIVIYQNDTMIVTKFKHEVQC
                     VICLPAALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQH
                     SYWKANFIERNIYDHSLMVSSNSIVHLKYHLEI"
BASE COUNT          789 a          374 c          244 g          888 t
ORIGIN      
        1 tgctttttgt gtatctgtaa cataaacaat aactgttctt tcgtcttgta aacttctttt
       61 ctttgataac aattttaaat cttttagctt ttgtaaagct ttagttaaat agtaaggttt
      121 gaactctgag cacttagcaa tctctttaga tgagatttcg ttagactcac ttcttaaaat
      181 atgatttaaa atataaattt cttcatagtt caaattgaac ttcttttttg tatctctgaa
      241 aaacttctta acttgaaatg ttgcgttgac taaatcatta atgtcattaa ttttactcat
      301 tattaaacca ctcctctgat gcacatcttg ttgtagcaag gttcacatgt aatttattaa
      361 atcgaaactc tatccaacta tgttataaag ttcattctaa acaaataagt ataaaaattc
      421 aatattttta ttactagaat atggttaaat acatttattt cttataataa tttgatgttt
      481 aagatatttt gccaaattga aaattattag tctgaatatt ttgtatctta actgatatgt
      541 aaacttttaa aaatttaatc tatataaacg tgtttgattt gatatttttt atattaattt
      601 gatggcttgc tcacaaaatt tataaaatat aaaaaaagaa gagctttaaa tcataacgca
      661 tgccatttat ctttaaatgc aatgagttat cattcaaact cttcttatca acctacatac
      721 attgacatta tactatcata tgttgtttta catattcaac aaacatttca acgttatcac
      781 ttttaatatc atgcattggg acattaccaa tattaaattt gaacattata gtttgaccat
      841 cataatctat cttctctatt tcattataac taatacttct ataataaaat tggccattca
      901 tatctacatt cataattaat ctttcatttg ttgctataaa tgccccttca aattcattct
      961 caccttgaac ttgatactca attattccta acactgacgg accctttttt tcagttggaa
     1021 ataaatcatt tggattcaca ttatctaaaa tcataatatc cctcccactt aaaactataa
     1081 actattcttc ataggtatat gaaatacaag tgattaacta tttattatta agcttaactt
     1141 gtattccctt ttcaagataa ttataacatg cttgaataag catagtgact ttagaaaact
     1201 tttctcgctt gattgttaca ataacacgcc tttcatctaa ttcacatctc tctttttaac
     1261 taatcccttc aaaatcaaac actttaaaat cggactaacc gcaaatatcg ggtgatggaa
     1321 ttcaccctgt aattctttaa ccgtcaattg acctttatgc agatttaata ttcctaaaac
     1381 gtataactct tctaatgata attgacacat atttaaataa cactttagat ttcgaaataa
     1441 atcttgtgac gacaataaat acgatacata gtcacaacta aaatcaaatg tatattgacc
     1501 aaaataacgt tcaatatagt cactaagctc attattcata tattctattt tatctatatg
     1561 cattttatta atcgaaatag tcaatttacg ttgatccctt tgatctcttc ctttcaatag
     1621 ccattgatga ttgtttaaat aacttaacat ttgaagaagg gccatcttag attggatttc
     1681 tttcattttt aataacctat ctattgaagc gctattggaa tcagaattat catatttaac
     1741 taatagattt aatagtttca cttgagtcaa agtgagatga aatgaatatt tcaacatatc
     1801 attaatgact ttcaacatta gtatgtgtgc agttaaacgc ttaactttaa atttagacat
     1861 ctttaaaacc tctcttaaac catgcctata tctcaagatg atatttcaaa tgaacaatac
     1921 tattgcttga gaccattaat gaatgatcat aaatatttct ttctataaaa ttagctttcc
     1981 aataactgtg ttgttgcata atatcattca caagtacacc attttcggaa gtatgattat
     2041 ctttatctat acttaaaaca atttgtttag ttttagcatg gctaaattgt tgaagaccct
     2101 tacacgataa acgtatagcg tctgaattct catttaacaa tgcggctggc aaacaaatga
     2161 cacattgtac ttcatgtttg aacttcgtta caatcatcgt gtcattttga taaataacaa
     2221 tccctcgtaa tcgattaagt atatttatga taaaaatgaa tattgataat ctagtcaaat
     2281 ttatttcttt gctcg
//
LOCUS       DAHHRR010000200         2235 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.204, whole genome shotgun sequence.
ACCESSION   DAHHRR010000200 DAHHRR010000000
VERSION     DAHHRR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.204"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            32..448
                     /locus_tag="NBI42_002607"
                     /pseudo
     CDS             32..448
                     /locus_tag="NBI42_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120900.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
     gene            577..825
                     /locus_tag="NBI42_002608"
     CDS             577..825
                     /locus_tag="NBI42_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778963.1"
                     /translation="MLILKIKEINDKSVTYKYFPNNDGNIKPGIIQMDIDSLEVINAE
                     KSSLEKNTRDNYFIHAIDRIYINTSKGLFPESELVAWG"
     gene            956..1207
                     /locus_tag="NBI42_002609"
                     /pseudo
     CDS             956..1207
                     /locus_tag="NBI42_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282612.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            1256..1602
                     /locus_tag="NBI42_002610"
                     /pseudo
     CDS             1256..1602
                     /locus_tag="NBI42_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1613..1807
                     /locus_tag="NBI42_002611"
     CDS             1613..1807
                     /locus_tag="NBI42_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778964.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETVIKSIRLLK"
     gene            1925..2235
                     /locus_tag="NBI42_002612"
                     /pseudo
     CDS             1925..2235
                     /locus_tag="NBI42_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451275.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
BASE COUNT          936 a          239 c          395 g          665 t
ORIGIN      
        1 actcaacgga aaaatattta aagttaaaga tatgatacca ggtgtgaatg cgccacctat
       61 gcatccttgg tgtagaagta ccacagtgcc atatgtaggc aattggcgag acaagttctt
      121 taaagagcgt gaaggtaaac acaaaataga aggttctttt attgaatcgg gtgcacttaa
      181 taataaaagt gacgaatacg gtatcaaaag gaacagacat gctcaaattt attataattc
      241 agttagaaat cgagataaac agatagagat ttctaaaatt gctaaaaata ctaatataaa
      301 taaaaataca attctaagag tttatgaaca tatattttaa aataagtatt tattagaaag
      361 tggatttaaa caatttgatc ctgactttta tatggttcaa cgttggcaac gtttaagaga
      421 agggaaaaat attaaaaaat ggatataata atgttgaaac atgaagctct tgaacactat
      481 ctaatgaata agtataattt gcactatata gaggcacata aacttacaga aataaaatat
      541 aattatagta ttttaattaa ttagaggtga aaatgattgc taatattaaa aataaaagaa
      601 attaatgata aaagtgttac atataaatat tttcctaata atgatggaaa tattaagccg
      661 ggtattattc aaatggatat agatagtctt gaagttatca atgctgaaaa atctagttta
      721 gaaaaaaata caagagataa ttattttatt catgccatag acagaatata cataaatact
      781 agtaaagggc tatttcctga atctgaactc gtggcatggg gataaagaat acaagaaagt
      841 aatagaatta gaaaataaga ataaagaaca aaaatttgaa aaatagcatc ctttctacac
      901 aaaaatatag aaagtggtgc tattttaata cattttttaa ctatttactg aaatatcaga
      961 atcacaaaag ttatcatatt taaaagaact tggtgaagat ggcgaatata aatatgttgc
     1021 caaaatagat agtaaaacat ctaaattatg tcattgactc aacggaaaaa tatttaaagt
     1081 taaagatatg ataccagctg tgaatgcgcc acctatgcat ccttggtgta gaagtaccgc
     1141 agtgccacat gtcggcaatt ggcgagacaa gttctttaaa gagcgtgaag gtaaatatca
     1201 agtagaagta aaagaagcaa aattacagga aatagctaaa agccagatga aagaaatgat
     1261 tgatagtggt aaaataaaaa tagaaataaa ttgtgaaaaa caaaatagac atatgttagg
     1321 tcatcaccta tataatgaaa ataaaaaaga gccattttaa gtaataagaa attgcctagc
     1381 tatacaatac tttctataga tctattgaat gaattgttaa gagaaaaaat gtcaacaggc
     1441 aatctaatat taagtgatga gcgatttgat ttgaaagaaa ttattaattt taatcaaatt
     1501 attggaaaag tacatatcga taatgtgtat attgaaacca gacaaggaaa agtacattat
     1561 tcgaagacag gtgctcatat agtaccttat attgataagt aggtgaaatg tattgagaat
     1621 tgaagatgca tatcgtaaag atgttattat tacgcttttg aataatgaag aatacgaagg
     1681 gtttgtaact gactatgaaa atgaattcga gagtgaaaca ggaaatcttg ttgtagatat
     1741 acagactgat tttgctgttt attcgtttga tgaaactgtg ataaaaagta ttagattatt
     1801 aaaataattt ctggatagta tcctttctac acggataaag agaaagaggt gctattttta
     1861 tacacttttt aactatttat tgaaatatca ttcactcaac ggtaaagtat ttaaagttaa
     1921 agaaatgata ccaagtgtga atgcgccacc tatgcatgct tggtgtagaa gtaccacagt
     1981 gccacatgtc ggcagttggc gagacaagtt ctttaaagag cgtgaaggta agtatcaggt
     2041 agagaataaa aaatatgaaa aagaaaaatt ataagaaaaa gctaaaacag aatttattga
     2101 tattccagtt ttgtttgatg atgaagggaa ttttagaaaa aagttataga ttataaaaag
     2161 ataataagta aatcttatgt gaatggtaaa tatatattga aacgaaatta ggtaaagttc
     2221 attattctaa aactg
//
LOCUS       DAHHRR010000201         2157 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.205, whole genome shotgun sequence.
ACCESSION   DAHHRR010000201 DAHHRR010000000
VERSION     DAHHRR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.205"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..160
                     /locus_tag="NBI42_002613"
     CDS             <1..160
                     /locus_tag="NBI42_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HCD1778965.1"
                     /translation="KLTLTNNKILIGKVIDFDDKVDNFDGYNSIEIDTGRITYDISEN
                     KIKTIVLQ"
     gene            complement(729..1754)
                     /locus_tag="NBI42_002614"
     CDS             complement(729..1754)
                     /locus_tag="NBI42_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HCD1778966.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVLSADTGHVAVHEGGAIEFSGHKV
                     ITLPSNEGKISASEVETYMETFKCDFKKDHMVFPGMVYISHPTEYGTLYSKSELEALS
                     KICKQYQLPLFMDGARLGYALMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDDLYFKISRHAIEMANKMKQGFIN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
     gene            2024..>2157
                     /locus_tag="NBI42_002615"
     CDS             2024..>2157
                     /locus_tag="NBI42_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778967.1"
                     /translation="MEQIKLKTFTAETLELLEKNINAFLSSEEAANLKLVNITIKEIE
                     "
BASE COUNT          778 a          354 c          280 g          745 t
ORIGIN      
        1 caaattaacg cttactaata ataaaatatt aattggaaaa gtgatagact ttgatgataa
       61 agttgataat tttgatggtt ataattcgat agaaattgat acaggtagaa ttacatatga
      121 tatatcagaa aataaaatta aaactattgt tttacaataa tagcatcctt tctacacaga
      181 taaagagaaa gggtgctatt ttttgtgctt ttataacaac taaggataca agagacatac
      241 cttcaacaat atcctcatct aaacctattg atttcctaag ctccatttta atcacaaagt
      301 ttatttgtaa agatgcacac ctataaaaat cttaatcatc gaaaagttac agacatgtcc
      361 atattaaaaa cttaaatagt gcattccatt taaattaaac atcgatattc ctaccaaaat
      421 agatttagca attcaattgt taatacgtca cgtctttcat aaaataacta caaccagcat
      481 aatacttctc aaatctcgta gaattaaagt aaatacagcg caaatttaca tttataaata
      541 tataaaataa aacaagatac caataatttg tagtatcttg ttttggaata atgtaatttt
      601 atatcatcaa cggcttcaaa tgaacagttt catatcgaca tgttcttata caagtcaatg
      661 cgtacaacta atccaactaa acctgcttaa ttaatacatg aatcttcaga tatcacacac
      721 tatcattatt aaatcaattc taatagttta tttaaatttt cttcggttgt cgcccagctg
      781 gttgcgaatc taacaacacg atgttgatca tcgtattttt cccaaacagc aaatttaact
      841 ttttgttcta actctgctat tttctcgtta cttaatataa aaaattgttg gttggtaggt
      901 gaatcaaagt aaagacgata gcctttattt ataaagcctt gcttcatctt atttgccatt
      961 tcgatagcat gtctacttat tttaaaatat aaatcatccg taaataattc tagaaattgt
     1021 atacctgtta aacgtccttt tgctaaaagg gcaccgtgat gtttgattcg agtggtaaat
     1081 tgttttggtt cattattttt tgtgaaaaca atagcttctc cgcaaagtgc accaatcttc
     1141 gtaccaccta tataaaatac atcacaatat ttagcgatgt ctttaatagt catatctgat
     1201 tgatcactca ttaatgcata tcctaaacgt gcaccatcca taaataatgg aagttgatat
     1261 tgcttacata ttttagaaag tgcttctaat tctgactttg agtaaagtgt gccatattca
     1321 gttggatgtg aaatgtatac cattcctgga aataccatgt ggtctttttt aaagtcacat
     1381 ttaaatgttt ccatataagt ctcaacttct gaagcgctaa ttttcccttc gttagatggt
     1441 aacgtaatta ctttatgacc actaaattca atagcaccac cttcatgtac agcaacatgt
     1501 cctgtatccg ctgacaatac accttcgtag ctttctagca tagaattgat gacaacctga
     1561 ttggtctgag ttccaccaac taaaaaacga attgtagcat ttggacaatc aatcgtatct
     1621 ttaatctttt caattgcctg agctgtgaat tgatcaaagc catatcccga agcttgtaca
     1681 agatttgtat ctactaatct ttttaatact ttttcatgag cgccttctaa ataatcattt
     1741 tcaaatgaaa tcactacatt tccccctaaa actaatatca gcattttaat aagataaacc
     1801 aatttcaaaa ctaattcgat atttaaaatg aattatgaat tgttaaagtt tgtatcgcat
     1861 tttcgcgaag ttgaataaat atattataca tcaaacaaca aattaataaa attaattaat
     1921 ttttaatcat aatgaccctt cgtgctcgaa cacacacatt aatgattgaa ttttactgtt
     1981 aaaagttcta tgatttagga aatagagaaa agaggggatt tagatggaac aaattaaact
     2041 taaaactttt acagctgaga ctttagagtt attagaaaaa aacattaatg cttttttaag
     2101 ttctgaagaa gctgcaaatt tgaaattagt aaatattaca ataaaagaaa ttgagga
//
LOCUS       DAHHRR010000202         2064 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.207, whole genome shotgun sequence.
ACCESSION   DAHHRR010000202 DAHHRR010000000
VERSION     DAHHRR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.207"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..1591
                     /locus_tag="NBI42_002616"
     CDS             <1..1591
                     /locus_tag="NBI42_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCD1778968.1"
                     /translation="KNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIKQMNIVVPLS
                     SLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTLIITQMLLTT
                     SVLWILNYTGNLDLFILRLVLLSCLFILTISVINLWTFLMLLNLNIANMIKGKQHFKT
                     IRFINTVCKSILLVLIASVMIENTSVIKDLNKIKETEKYWNVLDDYYTIEFAPYHETK
                     QSLIDNMLRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVNNQFWSIYHK
                     QFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKENKLSLQFINK
                     NDYRIFTFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFKNYDALVKNI
                     EKYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIILFDVKYYFEQ
                     HRKLLVIKKLYGYSTLRANYRYLLINNIVVVFIGILTNVILHSHYIMMIFATILVVQI
                     LLQICSLYYHGRRFNEVIKEF"
     gene            1594..1914
                     /locus_tag="NBI42_002617"
     CDS             1594..1914
                     /locus_tag="NBI42_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD1778969.1"
                     /translation="MKFIIAILLGLIVSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            1911..>2064
                     /locus_tag="NBI42_002618"
     CDS             1911..>2064
                     /locus_tag="NBI42_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1778970.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLE"
BASE COUNT          771 a          251 c          320 g          722 t
ORIGIN      
        1 caaaaatttt gacgtgtcta aattcataaa ttttttaaaa gaatatggtg ttactgctga
       61 atcatacaaa atagatcata tgatgattgc cgttggtgtc attaaacaaa tgaatatagt
      121 agttccgtta tcctcacttt taatcgttta ttttatttat tatattttcg aaaagaacat
      181 taatttcaaa gcgtatgcga ttaagtattt aaatggtttt acattaagaa aaataatttt
      241 tgaaaatttt tcaaagaaat gcacgtattg ggtaacgtta atcataactc aaatgctttt
      301 aactacaagt gtactttgga tattaaatta cacgggtaat ttagatttat tcatattaag
      361 attagttctg ctttcatgtc tttttatttt aacgattagt gttattaatt tatggacttt
      421 cttaatgtta ctaaatttaa atattgccaa tatgattaaa ggtaagcagc actttaaaac
      481 gattcgtttt attaatacag tttgtaaaag tattctctta gtactaatag ctagtgtaat
      541 gatagaaaat actagtgtta ttaaagattt gaataaaata aaggaaactg agaaatattg
      601 gaatgtatta gatgattatt atacgattga atttgcacct tatcacgaaa cgaaacaaag
      661 tttgattgat aatatgctgc gatcagaaca attagtaaag gctagtgaag cagaaaataa
      721 tgcgatttta ttcaaaccaa agggtgactc cgttgacaat gacaactttt cacctgatga
      781 ggggaatgta atattagtaa ataatcagtt ttggtcgatt tatcataagc agtttcaacc
      841 tgatattccg ataaaaaatc aaaaaaataa tgtcgaagta attattccac aaaagtttca
      901 tgcaatgcgt aatgaaatca atcaagcgta tcattcatgg tttgaatttg tacaaaataa
      961 aaataataaa gagaataagt tatctttaca gtttatcaac aaaaatgatt atcgaatttt
     1021 tacatttgat gcacgagata gtcgccattt gtcatttata gaggcgccaa tcattgtgaa
     1081 tgttcaggca tcagatttat cgaatgattt ttattatgcc atgattagtc aaggcggcta
     1141 tttattcaaa aattatgacg cactggtaaa aaatattgaa aagtatcatc ttgatgggga
     1201 aatcagtgga ataaccaatt ataaagatag cgtgatggaa atgtatcatg aaaataattt
     1261 gaaattaaca gtacttaact tttcacaaat cattatcgca atcattttaa taattattat
     1321 tttatttgat gtgaaatatt attttgaaca acatcgaaaa ttactcgtaa tcaaaaagct
     1381 atatggttat tcaacattaa gagccaatta ccgatactta ttaataaata atatagttgt
     1441 tgtttttatt ggaatattga cgaatgtaat tttacattct cactatataa tgatgatatt
     1501 tgcaacgatc cttgtcgttc aaatattatt gcagatttgc agcctatact atcatggccg
     1561 acgttttaat gaagttatca aggagtttta actatgaaat ttatcattgc aatattatta
     1621 ggattaattg tatccattac tattgctttt acaatcatac atcatcctat acttgatcgt
     1681 tttaatcctt tcttaaaaac ggagtatagt tatgccaaag tgcctaaagg tacgcaacaa
     1741 tatgttaata ttacggctta tagtgaaaga ggggaaaagc ttgattataa attaacattt
     1801 aatggatttt ctcctagtag aacgtatgtc gaaataaagc ataaagggca atatgttata
     1861 tcgatcacat atgttgaaaa agaggataca ccaaaagagg taagacaaga atgatagaat
     1921 taaaagattt aaccatacaa aaaggtaata cacatatttt gaagaaactt aatttgaaat
     1981 ttcaatgtgg aaaatcatat gcacttattg gtaaaagtgg atgtggtaaa tctacattat
     2041 taaatactat tgctggactt gaaa
//
LOCUS       DAHHRR010000203         2048 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.208, whole genome shotgun sequence.
ACCESSION   DAHHRR010000203 DAHHRR010000000
VERSION     DAHHRR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.208"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(67..537)
                     /locus_tag="NBI42_002619"
     CDS             complement(67..537)
                     /locus_tag="NBI42_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HCD1778971.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIENPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(585..1811)
                     /locus_tag="NBI42_002620"
     CDS             complement(585..1811)
                     /locus_tag="NBI42_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HCD1778972.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRNDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDSSLLRKEQNDTTDKDKSSNNTDDSNNSNNNNGVNNQQQPTTGQNTNQDQGN
                     IQQTPQASNNQNGVVN"
BASE COUNT          649 a          316 c          302 g          781 t
ORIGIN      
        1 ttagacataa aaaagctata aagatgattt atatgaatca tctttatagc taattaatta
       61 ttaatgttat agtgcttctt ttctaataat tgctgtatat tgccaaaaga ttcgcatttg
      121 agcaatattc caattattca ttcccattgt aaacccagaa ggtttaaata tattcacatc
      181 tgtcacttct aaagctgctg cgactaaata ttgtaatgga atactaagtg atttcattgt
      241 ttcggtaatg ccattttcat catataccca tgaaaatggt aactctgatt cgaaaacatc
      301 tacactattg aaaatttcag gtagggctaa aatataacat gcaccatcta caataggatt
      361 ctcgatacta tttttgtaat aaattaggag tgcttcatag ttttctcgat gctcatgatt
      421 gacgtaaaaa aattcacttc taaaatgagc catatcttgg ctatgaataa tttgttgttc
      481 taacatatta aacatcccat ttatatttgc aatcttttcg tatgtttttc tactcatgta
      541 tgatgctcct ttcataaata gtaaatgtta atcgtcatat aatattagtt tacaacacca
      601 ttttggttat ttgaagcttg tggcgtttgt tgaatattac cttgatcttg gttagtgttt
      661 tgacccgtag taggttgttg ctgattattg acaccattgt tgttattact attattactg
      721 tcgtcagtat tattcgaaga tttatcttta tctgtcgtat cgttttgttc ttttctcaat
      781 aaactactat ctaaaggcgt taatggtatt aatgaaccat aatggttgat aacacgttga
      841 tctaagaaat catttttatc attgataggt gataattcta aatcattacg aagtaagtta
      901 gcatattttt gaatgctttc aacgcttgga tgataatagt aaataccatt taacatatca
      961 tctttacctt ttagttgtgc agttttaatt tcaacatcat ttgttaagta cattttagct
     1021 aaagctttaa tttcagagtt agttaaatta tgcttagcat ttttacctac aatttgaatc
     1081 acattatcaa gtttatcaat agaatcaact tcctgtgctt tttggaataa aatcttaatt
     1141 aattccattt gacgttgtcc gcgtttcaag tctgaatcat gatgtctagt tctagcaact
     1201 gctaaagcct catcaccatt taatttttgg tacccttttt taattttaat cttaccagta
     1261 tcatctgtgt taggttcatt taagtcgtac ggcacatcat agtatatgcc accaagctca
     1321 tttacagcct cgacaaatgc tttcatattg actctcacat aataatcaac aggtacattc
     1381 atggtagctt ctaccgaatc cattgcggca attggaccac catatgcatg tgcgtgggta
     1441 atcttatcgt aatagccaac tttaggaatg tagctgatag tatcacgtgg aatactaagc
     1501 attctaattt gatgtttcga ttgattaaaa gtagttaaaa tcatagcgtc tgatctagag
     1561 tgttcagcat cctgtccttt ttttcttctt ccatcgttat catcgatacc taagaaaaga
     1621 atagagatag gttgttcttc gggattgact ttattatctc ttaagttgga ttgacgatta
     1681 gcatttttgc tgtcttgaga agattcgaat gcatcttggg acgttttaaa aagtaacgta
     1741 gcgaagacta ttggaacaac aatgagaacc aatgctagaa ggatcaaaaa gtattttaaa
     1801 aatttattca tgattgatgc tcctatatta aatttttgtt aaatcataat cataatttta
     1861 aaatataatt agttgaacga tatttataca acttcattac taatacatta tagccttaaa
     1921 ttgtagtact ttatagtaaa aaatacaaat gttatgtcaa agttccttgt aatttgaatg
     1981 ttttgttaaa atcaaaaagc atttttaaaa atataatgcg actttagtat gatgtcttaa
     2041 aaaattta
//
LOCUS       DAHHRR010000204         2020 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.209, whole genome shotgun sequence.
ACCESSION   DAHHRR010000204 DAHHRR010000000
VERSION     DAHHRR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.209"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            68..1006
                     /gene="lukS-PV"
                     /locus_tag="NBI42_002621"
     CDS             68..1006
                     /gene="lukS-PV"
                     /locus_tag="NBI42_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HCD1778973.1"
                     /translation="MVKKRLLAATLSLGIITPIATSFHESKADNNIENIGDGAEVVKR
                     TEDTSSDKWGVTQNIQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRW
                     PFQYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYS
                     KTISYNQQNYISEVERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRD
                     YFVPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGN
                     SYLEGSRIHNAFVNRNYTVKYEVNWKTHEIKVKGHN"
     gene            1002..1985
                     /gene="lukF-PV"
                     /locus_tag="NBI42_002622"
     CDS             1002..1985
                     /gene="lukF-PV"
                     /locus_tag="NBI42_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HCD1778974.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQF
                     YWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNG
                     SKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEM
                     FLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD
                     RYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS"
BASE COUNT          820 a          277 c          324 g          599 t
ORIGIN      
        1 ttattaaaag ttaaaaaggt ttaatataaa aatgtaataa aatttataaa gaaaggaaat
       61 gatttttatg gtcaaaaaaa gactattagc tgcaacattg tcgttaggaa taatcactcc
      121 tattgctact tcgtttcatg aatctaaagc tgataacaat attgagaata ttggtgatgg
      181 cgctgaggta gtcaaaagaa cagaagatac aagtagcgat aagtgggggg tcacacaaaa
      241 tattcagttt gattttgtta aagataaaaa gtataacaaa gacgctttga ttttaaaaat
      301 gcaaggtttt atcaattcaa agactactta ttacaattac aaaaacacag atcatataaa
      361 agcaatgagg tggcctttcc aatacaatat tggtctcaaa acaaatgacc ccaatgtaga
      421 tttaataaat tatctaccta aaaataaaat agattcagta aatgttagtc aaacattagg
      481 ttataacata ggtggtaatt ttaatagtgg tccatcaaca ggaggtaatg gttcatttaa
      541 ttattcaaaa acaattagtt ataatcaaca aaactatatc agtgaagtag aacgtcaaaa
      601 ttcaaaaagt gttcaatggg gaataaaagc taattcattt atcacatcat taggtaaaat
      661 gtctggacat gatccaaatt tatttgttgg atataaacca tatagtcaaa atccgagaga
      721 ctattttgtt ccagacaatg aattaccccc attagtacac agtggtttca atccttcatt
      781 tattgcaact gtttctcatg aaaaaggctc aggagataca agtgaatttg aaataacgta
      841 tggcagaaat atggatgtta ctcatgctac tagaagaaca acacactatg gcaatagtta
      901 tttagaagga tctagaatac acaacgcatt tgtaaacaga aattacacag ttaaatatga
      961 agtgaactgg aaaactcatg aaattaaagt gaaaggacat aattgatatg aaaaaaatag
     1021 tcaaatcatc agttgttaca tcaattgcat tgcttttgct atccaataca gttgatgcag
     1081 ctcaacatat cacacctgta agtgagaaaa aggttgatga taaaattact ttgtacaaaa
     1141 caactgcaac atcagattcc gataagttaa aaatttctca gattttaact tttaatttta
     1201 ttaaagataa aagttatgat aaagatacat taatactcaa agctgctgga aacatttatt
     1261 ctggctatac aaagccaaat ccaaaagaca ctattagttc tcaattttat tggggttcta
     1321 agtacaacat ttcaattaat tcagattcta atgactcagt aaacgttgta gattatgcac
     1381 ctaaaaatca aaatgaagaa tttcaagtac aacaaacggt aggttattct tatggtggag
     1441 atattaatat ctctaacggc ttatcaggtg gaggtaatgg ttcaaaatct ttttcagaga
     1501 caattaacta taaacaagaa agctatagaa ctagcttaga taaaagaact aatttcaaaa
     1561 aaattggttg ggatgttgaa gcacataaaa ttatgaataa tggttgggga ccatatggca
     1621 gagatagtta tcattcaact tatggtaatg aaatgttttt aggctcaaga caaagcaact
     1681 taaatgctgg acaaaacttc ttggaatatc acaaaatgcc agtgttatcc agaggtaact
     1741 tcaatccaga atttattggt gtcctatctc gaaaacaaaa cgctgcaaaa aaatcgaaaa
     1801 ttactgttac ttatcaaaga gaaatggata gatatacaaa cttttggaat caacttcact
     1861 ggataggtaa taattataaa gatgaaaata gagcaactca tacatcaatt tatgaagttg
     1921 attgggaaaa tcatacagtt aaattaatag atactcaatc taaggaaaaa aatcctatga
     1981 gctaaacaga tagataatca aaaaatctta aatatgttaa
//
LOCUS       DAHHRR010000205         2018 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.210, whole genome shotgun sequence.
ACCESSION   DAHHRR010000205 DAHHRR010000000
VERSION     DAHHRR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.210"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            163..528
                     /locus_tag="NBI42_002623"
     CDS             163..528
                     /locus_tag="NBI42_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCD1778975.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            710..1987
                     /locus_tag="NBI42_002624"
     CDS             710..1987
                     /locus_tag="NBI42_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCD1778976.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
BASE COUNT          709 a          265 c          347 g          697 t
ORIGIN      
        1 taaaatgaaa tttgttatta aattttttaa aagcatcgtg tatgtaattc gatttctata
       61 ataatattga gaataaaaaa atttgttaca atctggtaaa aactctttat taaataaatt
      121 gattcgttca tactgaatag ataagcaaaa aggagttaca taatgaagaa gaaaaaaaac
      181 gcgattaaag ttatcattat attatttatt tcattaattg ttgcagtagc atttttcttt
      241 ggtctgaaaa catatcaagg ccataagaat attcaactaa ttgacagtta cttggaagag
      301 aaaaatctaa aagataaaat taaatcagaa aaaacagagt atagtgctaa aaaaggtctc
      361 ttctataagg aagttacgtt taaagatgaa cctggtgtaa cttatgttgt gcaaccaatc
      421 agtactaata aaggattatt tgttgaaggc tttgatacag agactaagaa aagtttgaaa
      481 actgcaaagc ataaatattt taatcaaaat tataaaccat caaaatagtg tgacgtataa
      541 ctggtgaaac agtttagtgc cttcaatttt aattatttga ggtaaacagt ttttgtatgg
      601 aaattagtga tttttgacat ctataaaaga taaatatttt aattttttat agataattat
      661 caattttctg atataattga gtgatgtcag atgaagggga gtttgtacta tggctctatt
      721 caagagaaaa attagcctac ctatgcaagt tgttattgca ttagtgttag gtgttgtcgt
      781 aggactttta ttatatggac aagaaaacgt agcaaattac attaaaccat ttggtgacgt
      841 atttttaaat ttaattaaaa tgatcgttat accagttgta ttttgctcac tagcgctttc
      901 tatttcgaac gttggggaat cgaaaactgt agggcgttat ggctggaaaa caattttata
      961 ctttgaaatt attacaacaa tcgcaatagg tttagggatt atcttcggta acctatttaa
     1021 accaggtgct ggattagacc caacaaaatt acctaaaggt gatatttcta aatatcaatc
     1081 aactgcacat gcagcagaac aatctacata tggaaatcat tttattgata ccattgtaca
     1141 tattattccg acaaactttt ttgaagcttt aaataagggt gaattattac ctattatctt
     1201 cttcgcagta ttctttggat taggattagc tgcagtaggt aaaaaagcag aaccagttaa
     1261 agaattttta agcggatcgc ttgaagctgt gttctggatg attaataaaa ttttaaaatt
     1321 agcaccactt ggagtgtttg cattcatttg tactacaatt attacatttg gtgcatccgc
     1381 attattacca ctattaaaat tagtattagt tgttgtcttt gcaatggtgt tctttgtatt
     1441 cgctatacta ggactagttg catggatgtg tggtattaat atcatgaata ttattagaat
     1501 cttgaaaagt gaattgcttt tagcattttc tacatcaagt tcggaagctg tacttcctgt
     1561 aatgatgaag aaaatggaaa acttcggttc tccaaaagaa attacttctt ttgttatacc
     1621 aattggttat acgtttaact tagacggatc agcactttat caatctattg cagcattatt
     1681 cgttgcacag atgtatggaa tgcacttaac attatcagag caaattgtgt tgatgttaac
     1741 attaatgatt acatctaaag gtatggcggc agtaccagga acttcaatcg ttgttttatt
     1801 aacaacatta ggtgccatgg gcttaccggc acaaggttta gcattaatta ttggtgttga
     1861 ccgtatctta gatatggtac gtacatgtgt aaacgtaatt ggtaacgcat tatcaacaat
     1921 cgttatagct aaatgggaaa acgtatatga caaagcaaaa ggtcaagaat atttaaaatc
     1981 aatttaaaaa atactatctg acatttaaag ccccttac
//
LOCUS       DAHHRR010000206         2004 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.211, whole genome shotgun sequence.
ACCESSION   DAHHRR010000206 DAHHRR010000000
VERSION     DAHHRR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.211"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..829
                     /locus_tag="NBI42_002625"
     CDS             <1..829
                     /locus_tag="NBI42_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD1778977.1"
                     /translation="LKNDEKIKIIMNEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQ
                     ELVYELKAIALKGDVNHVIIEATGIAHPLELLVACQDPQIVNFFEKPIIYGVLDATRF
                     LERHQYTENTVSLMEDQLKLSDMIIINKIDLVTDDSLEKIDKQLSMICASIPTYKTTY
                     GQVSLEELDLPGKDREISSHHHHHHGIKSMTYTFTGPIDRQLFYQFIMKLPESVLRLK
                     GYVSFRDQPNAIYEFQYAYGLPDYGIIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            932..1840
                     /locus_tag="NBI42_002626"
     CDS             932..1840
                     /locus_tag="NBI42_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCD1778978.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVIQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
BASE COUNT          693 a          278 c          366 g          667 t
ORIGIN      
        1 attaaaaaat gatgagaaaa ttaaaatcat catgaatgaa ttcggtactt ttgatattga
       61 tagcaatagt atttcgaatg aaattgaagt tcattcattg attaatggtt gtgtttgttg
      121 cgatcttaaa caagaacttg tctatgaact aaaagccatt gctttaaaag gggacgttaa
      181 tcatgtcatc atagaagcga caggcattgc gcatccttta gaattactag ttgcatgtca
      241 agatccgcaa atcgttaatt tctttgaaaa gccaattatt tatggtgtat tagatgcgac
      301 tcgatttttg gaacgtcatc aatataccga aaatacagtt tcgctgatgg aagatcagtt
      361 gaaactaagt gacatgatta ttattaataa aattgatctt gtaactgatg acagtcttga
      421 gaaaattgat aagcaattaa gtatgatttg tgcaagtatt ccaacttata aaacaaccta
      481 tgggcaagtt tcgttggaag aattggactt acctggtaaa gacagagaga tatcgtctca
      541 tcatcaccat catcatggga ttaaaagtat gacttacaca tttacaggtc cgattgatcg
      601 tcaattgttt tatcaattca taatgaaatt accggaatct gttctacgtt tgaaaggtta
      661 tgtatcattt agagatcaac caaatgcaat ttatgaattt caatatgcat atggtttacc
      721 agactatgga ataattggca tgcaattacc attaacgatt gttattattg gtgaaacttt
      781 agatacaaat cacatacgta atcaattgga tatgctacaa tttacgtaaa tcagaaaatg
      841 tgttgtttca atttaacgga aatagcacat taatttctta atacttttat tgtgaatatt
      901 ttgtgacatt gagggatgga gtggatgaga tatggaacaa ataatgatta atcactatgt
      961 tcatttttct aggcttgtac aaggtttttg gcgtgcaaat gaatggaaga tgactgcgaa
     1021 agagttaaat tattttataa acgaattagt tgaacgtggg attacaacga tggatcatgc
     1081 tgatatttat ggggattatc aatgtgaatc actgtttggt aatgctttgg atttatcacc
     1141 cgaattaaga aataaaattc aaattgttac gaaatgtggt atcattttgc cttctaagca
     1201 atttgatttt acaaatggac atcgttatga tttgagtagt aagcacatcg tgaaatctgt
     1261 tgaacagtca ttaatcaatt tgaatgtaga ttatttagat agtctactca ttcatcgtcc
     1321 ttcaccattg atggatccag aacaagttgc tgatgcatta actaaacttg ttaaacaagg
     1381 taagttgaag tcattcgggg tgtcgaattt taatcattct caataccaat tgttaaatca
     1441 atatattatg aaagaaagac tacatattag catcaatcaa ttagaattat cgccatatca
     1501 cgttgatagt ttacaagatg gaacaatgga ttcaatgtat caaaaccatg ttcaaatcat
     1561 ggcttggagt ccttttgcag gcggtaaaat tttcgataag gaagatatta aagcgcaacg
     1621 tattatgaaa gttattcaat caatagctga caaatatggt gtgagtgaca cagctgtgat
     1681 gatagcgtgg ttagtaaaaa taccgcatcg tatcatgccg atacttggaa caagtcagtt
     1741 aaagcgtatt gatcaagcaa tcgaagggct gcaactaaat ttagatgatc agtcgtggtt
     1801 tgacatttac accgctatta tcggacaaga tattccgtaa acttatttac ttttaaatca
     1861 taaaggagca tatcatgaca aacgaagata aacgtttcga acaattaaga tttgaacgca
     1921 aatttatagt tattccgtat gtaatttatg cagtcattgt attactatta aatattttct
     1981 attctgattt gaaaataaca atga
//
LOCUS       DAHHRR010000207         1997 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.212, whole genome shotgun sequence.
ACCESSION   DAHHRR010000207 DAHHRR010000000
VERSION     DAHHRR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.212"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            80..>1997
                     /locus_tag="NBI42_002627"
     CDS             80..>1997
                     /locus_tag="NBI42_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCD1778979.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDWLSKHANDYDSDTYRTHMSRYESLSNQFEQLEGYQYESKIKTVLYGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPISIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHEKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQ"
BASE COUNT          795 a          286 c          347 g          569 t
ORIGIN      
        1 taaaatggtt attaatgtaa taattacgac ttttatcata agttaataaa atttgcgaaa
       61 gacgaaaggt gaagatcgaa tgatactttt acaactcaat catatatcaa aatcgttcga
      121 tggtgaagat atatttactg atgttgattt tgaagtaaaa actggtgaac gaattggtat
      181 cgtaggaaga aatggcgccg gtaaatcaac attgatgaaa attatagctg gtgtagaaaa
      241 ctatgattct ggaaatgtgt caaaaattaa aaacttaaaa ctaggttatt taactcaaca
      301 aatgacttta aattctaatg caacagtttt tgaagaaatg tcaaaaccat ttgaacatat
      361 taaacgaatg gaaagcttaa tcaaagaaga aacagattgg ttatcaaaac atgcaaatga
      421 ttacgatagt gatacatata gaacacatat gtcccgttat gaatctttat cgaatcaatt
      481 tgaacaatta gaaggatatc aatatgaaag taaaattaaa acagtacttt acgggttaaa
      541 ttttagtgaa gaagatttca ataaacctat caatgatttt agcggtggcc aaaaaacacg
      601 tttatcttta gctcaaatgc tattaaacga acctgattta ttacttttag atgaacctac
      661 taaccactta gatttagaaa cgacaaagtg gcttgaagat tatctacgtt attttaaagg
      721 tgcaatcgtc atcatcagcc atgatcgtta ctttttagat aaaatagtta ctcaaattta
      781 tgatgtggct ttaggtgatg tcaaacgcta tgttggtaat tacgaggaat ttatacagca
      841 acgggattta tattatcaaa aacgaatgca agaatatgaa agtcaacaag cagaaataaa
      901 acgattagaa acttttgttg agaaaaatat tacccgtgct tcaacaagtg gaatggcaaa
      961 aagtagacgt aagattttag aaaaaatgga acgcattgat aaaccaatgt tagatgccaa
     1021 aagtgcaaat attcaatttg gttttgaccg gaatacaggt aatgacgtca tgcatgtaaa
     1081 aaatttagaa attggttatc aaactgcaat taccaaacct atcagtatag aagtctctaa
     1141 aggcgatcat atagcaatca ttggcccaaa tggtattgga aaatcgacct taattaaaac
     1201 tattgctaat caacaaaaag cgcttaatgg cgatattact tttggcgcaa atttacaaat
     1261 tggttattat gatcaaaagc aagcagaatt taaatctagt aaaacgattt tagattatgt
     1321 gtgggatcaa tatccgttaa tgaatgaaaa agatattcgt gcagttcttg gacgtttctt
     1381 atttgtacaa gacgatgtta aaaagataat taatgattta tctggtggtg aaaaagcacg
     1441 tttacaacta gcactactta tgttgcagcg tgacaatgta cttattttag atgaacctac
     1501 caaccacctc gatatagatt caaaagaaat gttagagcaa gcactccaac attttgaagg
     1561 aacaatacta tttgtttccc atgatcgtta ctttattaac caattagcaa acaaggtatt
     1621 tgatttaaca attgatggcg gaaagatgta tttaggagat tatcaatatt acattgaaaa
     1681 agttgaggaa gcggaagcgt tgaaagcgca ccaagaagaa cagtcagtta acgtacaagc
     1741 acatgaaaag tctatggaac aatcgtcgta tcataaccaa aaagaacaaa gacgtgaaca
     1801 acgtaaactt gaacgacaaa tatccgagtg tgaaaatgaa atagaaactt tagagactac
     1861 tatcttacaa atagacgagc aattaaccca accagaagtt tataacaatc cacaaaaagc
     1921 aaacgaatta gctatccaaa aacaagatag cgaacaaaaa ttagaacacg ctatgtctaa
     1981 atgggaagaa ttacaac
//
LOCUS       DAHHRR010000208         1993 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.213, whole genome shotgun sequence.
ACCESSION   DAHHRR010000208 DAHHRR010000000
VERSION     DAHHRR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.213"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            173..1663
                     /locus_tag="NBI42_002628"
     CDS             173..1663
                     /locus_tag="NBI42_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HCD1778980.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDADRIATEEEYKFMIVNSQDDIDTELLDNYDMSDL
                     NHESIENYRKLLLKNTNDERYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFG
                     KYNAISDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNI
                     EESFSLNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGN
                     MRINKEDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELT
                     DMDTNVVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILK
                     KLKDDNLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
BASE COUNT          813 a          194 c          384 g          602 t
ORIGIN      
        1 cagaggaatc gtttgaaatc atcgtagtaa tcgcgcgaaa atcgcgtgaa ataatcagag
       61 gaatcgtttg aaatcatcgt agtaatcgcg cgaaaatcgc gtgaaatact atggtagacg
      121 gtttgagtaa attaatggag tattttaata tttatggttt ggaggaattg cgttgcatag
      181 agaaagtaaa tatatagagt ataagaaatc acgaaaagga ttatctaatg atatttggtc
      241 tacgtatagt gcttttgcaa atactgaagg tggtactata tatttaggaa ttgaagaaaa
      301 aaagatcgag gacaaaaaag tctttgtttc agttggtgtt gaagatccag agaaaatgat
      361 tgaagatttt tggaatgcac tatatggaag aagtaaagtt agtcaaaata ttttatcaaa
      421 taaagatgtt aaaattgtta atattgaaaa taaagcgtgc attgaaattc atgtaccaga
      481 agcgccttat tcgaagaaac cgatatatgt agataataaa aaagatttag tatataaaag
      541 agttgatgat gctgatagaa ttgcgactga agaagagtat aaattcatga ttgtaaattc
      601 tcaagacgat atagatacag aattattaga taactatgac atgtctgatt taaatcacga
      661 atctatcgaa aattatagga aacttctatt aaaaaatact aatgatgaga gatatgcgaa
      721 tatgagccaa ctggatttaa tgatagattt aggagcatat agaaaagata gaagttcgaa
      781 agacaaacag tataaaatga ctacagcatg tttattattc tttggtaagt ataatgcgat
      841 tagtgataga ttcccaggat ttcaattaga ttattttaag aaaacaaatt acctagatac
      901 tgattggaaa gatagaatat caagtggaga tttaggtaat gaagatttaa acgtgtatag
      961 tttttttgaa aaagtattga taaaattaac tgataacatt gaggaatcat ttagcctaaa
     1021 tgatggtttg actagacaaa attatgcaag agatttaaaa gtagcaattc gcgaagcact
     1081 ggttaataca ttaatgcatg cgtattatga tactaagcaa agtattaaaa tagttaattg
     1141 tgaagatttt atagagtttt ataatccggg taatatgaga ataaataaag aagattttat
     1201 tcatggaggg cattcaaagg acagaaatag tatattatcg acgcttttca gaagagtagg
     1261 atattcagaa aaagctggat ctggaggacc aaggatattc gatgtagtta atagacataa
     1321 gcttaaaacg cctgaaatag aattaacgga catggacact aatgtagtac tttggaaaca
     1381 agatttaatg aaggagtttg aaaaatatcc tgagttagac aaaaaagtaa taaagtatat
     1441 tattgactat ggatcaataa gtaagggtga agccttaaaa atggaaaata tgacagaata
     1501 tcagtttaga aatattttaa aaaaactaaa agatgataac ttgataaaaa aagaaggtga
     1561 aggtccggct actaaatatg tgttaataga atcaaaagaa gctgatatat tgcgaactaa
     1621 aaaagtaatt aaaagtttag agtctttctt taggaataaa taaaaaaaca agagataggt
     1681 gcgaagtgtt tggattatac aaatgcttcg catcttatat ttattaataa atatcataga
     1741 aaaccgtatc attaaccgat acgcagagat gcggtttttt agacacttca taaagggatt
     1801 ttgaacgtat cagaacatat gaggtttata ggaattgctg ttatgttttt tgatcacatc
     1861 aataaacaaa aaaggtatgt actatgtaaa atatttatta aatgatataa agcgagggta
     1921 tataaatgat ttttaaatag atatttatcc aataatataa aaaggaacta taagctatat
     1981 ctaaaagcta tat
//
LOCUS       DAHHRR010000209         1971 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.214, whole genome shotgun sequence.
ACCESSION   DAHHRR010000209 DAHHRR010000000
VERSION     DAHHRR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.214"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            188..604
                     /locus_tag="NBI42_002629"
     CDS             188..604
                     /locus_tag="NBI42_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778981.1"
                     /translation="MFKVNYSILSYYPDIYLHCNLAVGVAFEIIGESYHKNEIKFITQ
                     RRKILSFDDEIDNLELINMFLDGLKCEFEQTNQSLKSYKKKFVNNFYFENIEYKMFDS
                     LKEVNNFIEKTYKFVLHLGLDKKERLKKTEKELLTS"
     gene            747..992
                     /locus_tag="NBI42_002630"
     CDS             747..992
                     /locus_tag="NBI42_002630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778982.1"
                     /translation="MANIAKAKSEIFRNYTLGFATLATIIKMFFIEGDIPSLVLYYEV
                     MKMTNLTKTRIIIFINYLIGILALIFAFYIILMSIAV"
     gene            complement(1013..1240)
                     /locus_tag="NBI42_002631"
                     /pseudo
     CDS             complement(1013..1240)
                     /locus_tag="NBI42_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1231..1857)
                     /locus_tag="NBI42_002632"
                     /pseudo
     CDS             complement(1231..1857)
                     /locus_tag="NBI42_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215400.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1836..>1971
                     /locus_tag="NBI42_002633"
     CDS             1836..>1971
                     /locus_tag="NBI42_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778983.1"
                     /translation="MNSVERKEDDQVIVYEYIPQDKIEKGKGEKTVNKLDSKVIDYKL
                     S"
BASE COUNT          727 a          255 c          299 g          690 t
ORIGIN      
        1 atataagttt aagtaggtgc gagtgaagaa aataaaaaag ctcaaatgta ccaaattgtt
       61 aatcttaata aatctctact ttataaagat tgaatggaca ttcgagcttt aatgagtcag
      121 gagggacttt cccttctaca atttaataat aatacttgct tcaccactat acaaggagtg
      181 atttgttatg ttcaaagtga attattcgat tttaagttat tatccagata tttatctcca
      241 ttgtaattta gctgtaggtg tggcatttga aattatagga gaaagttatc ataagaatga
      301 aattaaattt ataacccaaa gaagaaaaat tttatcattt gatgatgaaa tagacaattt
      361 agaacttatt aatatgtttt tggatgggct taaatgtgaa ttcgaacaaa ctaatcaatc
      421 attaaaaagt tataagaaaa agtttgttaa taatttctac tttgagaata ttgaatataa
      481 aatgtttgac tcattaaaag aagttaataa ttttattgaa aaaacatata aatttgtatt
      541 acatcttggt ttagataaaa aagaaagact taagaaaact gagaaagaac ttttgactag
      601 ctaagtaaaa aggtcaaaca tattgttctg aatataaata agtaataact gtatctttat
      661 tgatgcggtt attttttatg taaaaaaccg aaaaaagtta tacaaaaacg tgttggatac
      721 aggacagatt agaaagagaa gaaaaaatgg caaatatcgc aaaagccaaa agcgaaatct
      781 tccggaacta cactttaggc tttgcaacac ttgccacaat aataaaaatg ttttttatag
      841 agggggatat cccctccctc gtattatatt acgaggtgat gaaaatgaca aacttaacta
      901 aaactagaat aataatcttt attaattatc taatcggaat tttagcttta atatttgcct
      961 tttatataat cttaatgtca atagcggttt aaaaatgtcg ttttttagcg gtttaagatt
     1021 gtcgcatatt taaggatttt tcgtttttat aatcttttaa tcgatatgat tctccagtaa
     1081 ttttaaatac ttttgaatgg tgaactaacc gatcaattat agctgctgat acaatcttat
     1141 tactaaatga ctctccccaa ctagaaaatg taatattagt cgtaattatt gttgatttca
     1201 tttcatatct tagtgacatt aactggtaga ataaatcagc aattcaccag tagaattatt
     1261 gaatatcatt tttaattctt caccaatgta tttagtaggt accgagtact tatcatttct
     1321 aaagttaatc atcgattctt tagaaacgat tcttatacac tcattttcga tataagtatc
     1381 tagtaacttg gcattaaaag aatttaaatg ctctttttct tcataattga aaacgtcgat
     1441 aggtcgttgt tctgttgctt gtgaaatttc taagtgattc aattcgtgac ataaatcgtt
     1501 tactagccca ataagttcta cagcatcata aaattcataa tcgtatggtc ttaaacgctg
     1561 ttcaacaaat ttagctaatg attcaacaga cccttttgtt tgaggacgat agggtctaca
     1621 agcaataggt tcaaagttgg cattcttact aaattgataa aataaattat taaaggcgac
     1681 ttttttatat tgtgttctcg gtcgatcaac tacagttctc atattatcga accagattta
     1741 ttatggaacc ccttcggtgt attcaaaagc gtcttttgaa cattcaaata aggtatcttg
     1801 ttttctatcc caagttaatg tgatatactt catttttgaa tagtgtagaa cgtaaagaag
     1861 atgatcaagt tattgtatat gagtatattc cacaagataa aatagaaaaa gggaaaggtg
     1921 aaaaaacagt aaataaatta gattcaaagg taattgacta taaattatca a
//
LOCUS       DAHHRR010000210         1965 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.215, whole genome shotgun sequence.
ACCESSION   DAHHRR010000210 DAHHRR010000000
VERSION     DAHHRR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.215"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..878
                     /locus_tag="NBI42_002634"
     CDS             <1..878
                     /locus_tag="NBI42_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1778984.1"
                     /translation="KQAGASLFETGSVDDTLILADQVKKYENSSALQKRITSGNFFIK
                     FNQKKVEEFKNQDLRMAIALAIDKKGYVEAVKNDGSIYSNTFTAKGVAKLSNGDDYIS
                     SINSPLKYDKKEALKHWEKAKKELNLKNFTFSMNTEDTPDSKISAEYIKAQIEKNLPG
                     VKMKIRQLPFKQRLSEEFKMTYETSLTGWAADYPDPTTYLSTMTKDNPQNNTDWSNKK
                     YDDYLGEINGELLGEVSKRDNRMKEDEELLLNDVPLAPIYQKGEAHLTNPKVKGLQYH
                     VVGPDTTLKYVYIDK"
     gene            complement(998..>1965)
                     /gene="trpS"
                     /locus_tag="NBI42_002635"
     CDS             complement(998..>1965)
                     /gene="trpS"
                     /locus_tag="NBI42_002635"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCD1778985.1"
                     /translation="QPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHAITMPQDRL
                     KLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGELERMTQYKD
                     KAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVDRFNSRYND
                     VLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKIKSAVTDSD
                     GIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEIVKAFLVEF
                     QEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
BASE COUNT          698 a          309 c          313 g          645 t
ORIGIN      
        1 ataaacaagc tggtgcttca ttatttgaaa caggatctgt tgatgataca ttaattttag
       61 ccgatcaagt aaaaaaatat gagaattcta gtgctttaca aaagcgtata acttcaggta
      121 attttttcat aaaatttaat caaaaaaagg tcgaagaatt taaaaatcaa gatttacgca
      181 tggcaatagc attagcaatc gataaaaaag gatatgttga agcagttaaa aatgatggtt
      241 ctatatacag taatacattt acagccaaag gtgtcgcaaa acttagtaat ggtgatgatt
      301 atatatcaag tattaattca ccattaaagt atgataagaa agaagcgttg aaacattggg
      361 aaaaagcaaa gaaagagcta aatttaaaaa acttcacatt ttcaatgaat actgaagata
      421 caccagattc aaaaatctca gcagagtata ttaaggcaca aatagaaaaa aatttacctg
      481 gtgtaaaaat gaaaattaga caactacctt tcaaacagag attaagtgaa gaatttaaaa
      541 tgacatatga aacatcctta actggttggg ctgctgatta tcccgatcca acaacttatt
      601 tgtcgacaat gacaaaagat aatccccaaa ataatacaga ctggagtaat aaaaaatatg
      661 acgattattt aggtgaaata aacggagaat tattagggga agttagcaag agagataata
      721 gaatgaaaga agatgaagag ttattgttaa atgatgtgcc tctagctcca atttatcaaa
      781 agggagaagc acatttaact aatcctaaag taaaaggtct tcaataccat gttgtaggtc
      841 cagatacaac attaaagtat gtatatatag ataaataata acttttaaag gctaataaga
      901 tgtgattata aaaaatggac attgtgaaaa acagcagtaa gataatttca attagaagtt
      961 tacttactgc tgttttattt ctgtattaat gtttatttta tctcttacgt cctaaaccca
     1021 tcgctttttc cattttcttg acagttttaa atgaaacttt gtgtgcttta tctctacctt
     1081 gatctaaaat atcatcaagt ttatctgagt tatagaaact ttcgtatttt tcttggaatt
     1141 ctactaaaaa tgctttaact atttcagcaa ggtcaccttt aaatttacca taaccttcac
     1201 cttcatattt tgcctcaata tctttaattg gcatgtctgt taatccagcg tatattgaaa
     1261 ttaaattcgt tatacctggc ttgttgtcac gatcaaattt aataatacca tctgaatcag
     1321 ttactgcgct tttaattttt ttagctgcaa cattcggctc gtctaataat gaaatgaagt
     1381 ttttagcatt atcatcactc ttactcattt ttcttgttgg gtcttgtaaa ctcatgacac
     1441 gtccaccaac tttaggcata cgaatttcag gtttcacaag cacatcatta tagcggctat
     1501 taaatctatc tacaaggtta cgagtcaatt cgatatgctg cttttggtca tcgccaactg
     1561 gaacgatatt agtattgtaa agaacaatat cagctgccat taaaggtgga tatgttaata
     1621 gaccagctgg tataccttca actgctttct gagctttatc tttgtattgc gtcatacgct
     1681 ctaattctcc aacagaagca atcgtagtta acatccatcc tgcttgtacg tgtgcaggga
     1741 cttcagattg tatgaacaat gttgctttgt ctggatctat accagaagct aaatatattg
     1801 ctgctaattg tcttgtttgt ttacgtaatt tcaaacgatc ttgtggcatt gtaattgcat
     1861 gttgatcaac gatacagaaa taacaatcat agtcattttg cacatcaaca aattgtttta
     1921 atgcaccaat ataatttcca atagtaggaa ttccactagg ttgaa
//
LOCUS       DAHHRR010000211         1965 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.216, whole genome shotgun sequence.
ACCESSION   DAHHRR010000211 DAHHRR010000000
VERSION     DAHHRR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.216"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(50..532)
                     /locus_tag="NBI42_002636"
     CDS             complement(50..532)
                     /locus_tag="NBI42_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCD1778986.1"
                     /translation="MILISSCLIGENVRYDGGNQLNVRLKELIDSGKAIHACPELLGG
                     MLIPREPAEIIGGDGFDVWNNVAKVITISNKDVTDDYKYGAIATLKILKKYQCDTVIL
                     KANSPSCGSQEIYDGNFTGNKKKGVGVATALLINEGIKVYDENSFFDQNMIETIVHEK
                     "
     gene            complement(579..929)
                     /locus_tag="NBI42_002637"
                     /pseudo
     CDS             complement(579..929)
                     /locus_tag="NBI42_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2992 family protein"
     gene            1349..1501
                     /locus_tag="NBI42_002638"
     CDS             1349..1501
                     /locus_tag="NBI42_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778987.1"
                     /translation="MVPEEKGSITLSKEAAIIFATAKFKPFQNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            1762..>1965
                     /locus_tag="NBI42_002639"
     CDS             1762..>1965
                     /locus_tag="NBI42_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1778988.1"
                     /translation="MTKERRTFSSEFKLQVVRLYENGKLKNEIIREYDLKPSIFSNSI
                     KQHQNTGSFNHQDNLKSDEKELIK"
BASE COUNT          689 a          344 c          228 g          704 t
ORIGIN      
        1 tcataatgaa aatttttgta gtattttaga ttaaaaaatg attcaatcct tacttttcat
       61 gtactatcgt ttcaatcatg ttttggtcaa aaaacgaatt ttcatcataa acttttatac
      121 cttcattaat gagtaaagca gttgccacac ctacaccctt tttcttattt cctgtaaaat
      181 tcccatcata tatttcttga gatccacaag atggactatt tgcttttaaa ataactgtat
      241 cacattgata ttttttcaaa atttttaatg tagctattgc tccatattta taatcatcag
      301 ttacgtcttt gttcgaaata gtaataacct ttgcaacgtt attccacaca tcaaaaccat
      361 cgccaccaat aatttctgca ggttctctag gaattaacat tccaccaagc aattctggac
      421 atgcgtgaat agcctttcca ctgtctatta attctttcaa tctaacattc aattgattgc
      481 caccatcgta tcttacattt tcgcctatca aacacgaact aattaaaatc atcccaacca
      541 cctataaatc attttgtttt aatttatttg tcatattgct aatgcccctt atgcttctct
      601 tttgctttaa cctttttaat ttctcttttt cttttttgaa atgcatcacg tttttattta
      661 tagcgcttcc gtttttgttt tttctttagt tctaattctt tcttaatagc tatttgtact
      721 tgggtagagt atttaggttt cttttgctcc ttagcaattt gtctttgcag ccttttcggg
      781 tttacttttc tagaaatatt ttttgtataa acaatagttt gcacatcatc aatcattttt
      841 attaattgat gatgaataaa atctaatacc tctatatcat caggttcatt cccaaatgtg
      901 tattggataa atttagattt attttccatt ctatattcta ctaatgctac aaaaaattgc
      961 ccattgtaaa aaaatagata gctccatgct atccctcctc tagttttatt agaaatgacg
     1021 gacaacccga ggaggaaggt tactgacagt tttaaaactg cattgggact accaacccaa
     1081 ttgtgttttt acacttctat atacagaata tatgccacaa cgttccatgt aaactgtatt
     1141 ttaaaaattt acattacaga acataaatgt aaataatgct aataaaatat tcacagggaa
     1201 atacttacac cccattggtg ttgattggca ttgcataacg cttttaaaaa aatcatttgg
     1261 taacaataag aaactattta cacaacctat tttgaatagt aaaattattt tataagattt
     1321 aaatctaatt gtacaaggag taaatatcat ggtaccagaa gaaaaaggtt ctattacttt
     1381 gtcaaaagaa gcagctatca tatttgcaac tgcaaaattc aaaccatttc agaacagaat
     1441 taaaaataac ccacaaaaaa caaatccatt ccttaaatta catgaaaaca aaaaatctta
     1501 atcactttat ttatagcatt tctaatctca gaaatgctat aaaggacaac gccctctttc
     1561 ataactatat aagtcaagaa accaggaaat tccaaacatc tttaaaccct tcaatagcac
     1621 attccctaaa gcataaatca atatcaatga acgatcaaaa ttccttatgt acttatgatg
     1681 tgtcaatgaa tcgtctacac ttaattgaac aaattctatg agaatagata ttgttaaatt
     1741 aagaatgtag tcaattttat tatgacaaaa gaaagaagaa catttagttc agagtttaag
     1801 ttacaagtgg ttagattata tgaaaatggt aagcttaaga atgaaattat acgcgagtat
     1861 gatttaaaac cctcaatttt ctctaattcg ataaaacaac accaaaatac tggatccttc
     1921 aatcatcaag ataatttaaa aagtgatgaa aaagagttaa taaaa
//
LOCUS       DAHHRR010000212         1960 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.217, whole genome shotgun sequence.
ACCESSION   DAHHRR010000212 DAHHRR010000000
VERSION     DAHHRR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.217"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            35..889
                     /locus_tag="NBI42_002640"
     CDS             35..889
                     /locus_tag="NBI42_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1778989.1"
                     /translation="MSFYLVLIIIIVVLTGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLEEGKLKYYDIIVTSNLAT
                     KHPFFEFARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEMSNSNIEKVVGH
                     YISQQYHDQFNSSRSAIYTFTETVQPNRVVYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            complement(1032..1565)
                     /locus_tag="NBI42_002641"
     CDS             complement(1032..1565)
                     /locus_tag="NBI42_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCD1778990.1"
                     /translation="MKSNKSLAMIVVAIIIVSVLAFQFMNHTGPFKKGTNQESTQDLK
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPYGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWLSKDHMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            1704..1892
                     /locus_tag="NBI42_002642"
     CDS             1704..1892
                     /locus_tag="NBI42_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HCD1778991.1"
                     /translation="MNISNENTVTLIAVIIAIIVGIFLQIFFKLPLIVSAVLSILLGI
                     LVGFIVYLIASFYKKRKK"
BASE COUNT          672 a          296 c          282 g          710 t
ORIGIN      
        1 aaagttaaag aagtcaaaaa ggattggtga aaaaatgtct ttttatcttg tacttatcat
       61 tatcatagta gtattgaccg gtattttagt tttaaatcaa aggtatagta acagtaaaat
      121 cgataccgag gtctatgcta gaaaacaact cattaaaaag aacaaagcat taagtgctga
      181 aaatgctgaa ttaagaagtc aaatgttgag ttctaataat gatgttgggc atcacgctta
      241 taaaaatgct aaaagagaat tacgtaagat tttagatagt tatcttgaag aaggaaaatt
      301 aaaatattac gatattatcg tgactagtaa tttagcaaca aaacatccat ttttcgagtt
      361 tgcacgttca tttgatttta tcattgtttc agatatcggt ttaataaatg ttgacgtgaa
      421 aagttggggt gaaaaaacat tttatcactt cgatgtacca gatgaacatg atacagaaat
      481 gagtaatagt aatatagaaa aagttgttgg tcattatatt agccagcaat atcacgatca
      541 gtttaattca tcaagaagtg cgatttatac atttacagaa actgttcaac caaatcgtgt
      601 tgtttatgat ttttatgatt atgatccata tcaattagca gcgaataatg caaaagcatt
      661 gaaggatcat attgaacaaa actttaactt taaagtccaa agtaccggtg ttatttattt
      721 tagtgatggt acagttaata tcattcaagg ttctgaagaa agagataaat atgttgatac
      781 agtttcaaca aaatcttcgt tacgacgtat tattagcgaa gccatagagc tatctaaaca
      841 ccctttaaat aaagaacagg ttgatcaaat cacagctata ttcaagtaaa atcaaaggca
      901 taataatcca ttacgctcga tacgcactat ggttattatg cctttaaatt atttctatat
      961 ataaatttat ggtaataata aatcgatgct tcaagtatat tttactttaa tatatataat
     1021 cacctattta attatttact ccaaatattt aatttctgtt gtttagcttt attttgtgct
     1081 tctataaata catttctata tttgccattt ggtgaaaaat acttctctct agcaagcccc
     1141 ttttccacta attccttatt gtacatatgg tctttactaa gccatacata tgccaatgtt
     1201 ctaccatagc gatcttgctt ttctttatca tattctaaat aaacatcctg attcgttaat
     1261 gtcttcttac tataatttga tgcttcttta ccatatggct gtacaggcgt attcggtttt
     1321 acagtttcag gagtatcaac tccaatgagt ctaaccttga tttccttacc gttttgattc
     1381 gctataaatg tatctccatc aacgactctt tgaacatgta ccttgtcctt accttttaaa
     1441 tcttgcgtac tttcttgatt tgtccccttt ttaaaaggac cggtatgatt cataaattgg
     1501 aacgccaaaa cacttacaat aatgatggcc accacaatca tagcaagaga tttattcgat
     1561 ttcattttgc cacctacttc tcttttttca ttccgattaa tcatataaaa gttaagaaat
     1621 atcgtcaata gccatatcta tatatttcaa tattttgtaa tgctatagca tgatataata
     1681 attttattaa ggagtgaaaa tgaatgaaca tttcaaatga aaatacagtc acattgattg
     1741 cagtaattat cgcaattatc gttggtattt ttctacaaat atttttcaaa ttaccattaa
     1801 tagtaagtgc agtgttatct attttacttg gtattttagt tggatttatt gtttacctta
     1861 ttgcctcgtt ttataaaaaa cgaaaaaaat aattcaacaa ctagtgtcgt taatgtcaaa
     1921 ataaaagtat tgagtatgat atatgtactg gttcattgcc
//
LOCUS       DAHHRR010000213         1940 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.218, whole genome shotgun sequence.
ACCESSION   DAHHRR010000213 DAHHRR010000000
VERSION     DAHHRR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.218"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(65..709)
                     /gene="trmB"
                     /locus_tag="NBI42_002643"
     CDS             complement(65..709)
                     /gene="trmB"
                     /locus_tag="NBI42_002643"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCD1778992.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNIKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            complement(724..1515)
                     /locus_tag="NBI42_002644"
     CDS             complement(724..1515)
                     /locus_tag="NBI42_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCD1778993.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHMPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
BASE COUNT          619 a          373 c          248 g          700 t
ORIGIN      
        1 tatagaacca ttatagtaca aaaaaacact tataattgta tgtatacata ccctcataag
       61 tgctttattt ttgtgaatgg aatttcgctt ccatacgata aatacgtgac cctttatccg
      121 aaaatttctt ttcatattct gttaaaatat tactgccatc gtcttcttga tgtaaattta
      181 gatttatttt tgtaaaatac attccaaatt gagacatact ttctaaactg taggcaaata
      241 gtcctctgtt atcagtttta aaatgtaaat ctccttcatc atttaagatt tgttgataca
      301 acgctaaaaa cgtatgatac gttaaacgtc gttttgcatg acgatttttt ggccatggat
      361 ctgaaaagtt caaataaata cgtgaaactt cgccgtcttt aaaatattca tttaattcaa
      421 tggcatcatt acaaataatc tttatatttg ttaaacccat ctctttaact ttatccaata
      481 ctttataaac gatacttttc tcacgttcca ttgaaatata gttaatatga ggattttgag
      541 cagctaatgt tgtaataaac tgccccattc ccgaaccaat ttcaatgtgt atcggttgcg
      601 ttttatcaaa ccattcagtc attttccctg catgttgacc gtccatgtca accaattcag
      661 gatgatcttt taaataatct tcagcccatg gtttgtatcg aactctcata ttttattctc
      721 ctcttaaata aacatgttac tattcataac ttcatttagg aattttagcc aagtgttcat
      781 atccttatat cttttttgct cttcatacca ttgaacaaga cctatagatt gaattaccgt
      841 ataccatttc atacgcttat ttaaattcaa gctctcttga acaccatatg tttcaagcca
      901 ttcagaccat tgttgttgtg gaacatagtt gtaaagcagc attccgatat caattgccgg
      961 gtctgcaatc atagcgcctt cccaatcgac taaaaatagt tcatcacgat cagataataa
     1021 ccaattatta tgattcacat caccatgtac aactgtgaaa aaacgtgaat ctaaactcgg
     1081 catatgctct tccaaatagg ttaatgattt tctcacaata tgatgtgtta aaacttctct
     1141 tgataaagag gcattaattt tattaagcat aatctcagga gtaatgggtt ccatttccat
     1201 acgctttaac atacttaata aaggtctaga attgtgtatc ttctttaata aatgtgcaac
     1261 tcttgtttgc ttcatttcat ttgaagatag ttcacggcca tttttccaat gttgtgctgt
     1321 aacaacctcg cctgtttcta tgcgtttcgt ccatactaat ttgggcacaa taccttctgc
     1381 tgataatgcc gcaataaatg gatttgaatt tcgttttaaa aacaactttt gtccatcttg
     1441 ttcagccata tatgcttcgc cagatgcacc acctgcagaa tcaagtgtcc accctaattg
     1501 ataaaactgc tccaactcgt ccacctcact ttcaattaga aaatggctct agaaataggt
     1561 ttttcaagag ccatatattc taatttataa caccatactg gtacaaatat tatgtccaga
     1621 taattatttt aaatcctcaa ccaatgccta cattacacga ctaaatttaa atcgtaatgt
     1681 ctgtcattga caccatacat tctatagtca cttacttgac ataaaatgtt accgtgtcta
     1741 aaactacaag tttttgtaac ttttgtaggc tataaactct agttttcaaa ataattgcta
     1801 tcccattttc atggttagca taaatttatg aactgtaaca tttacgtact tagtaaaata
     1861 tgatgcacat catatttgta actcatagaa aattttataa tttttatcat tatatttcaa
     1921 ctgaaaatga gaaacaaaat
//
LOCUS       DAHHRR010000214         1926 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.219, whole genome shotgun sequence.
ACCESSION   DAHHRR010000214 DAHHRR010000000
VERSION     DAHHRR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.219"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            86..913
                     /locus_tag="NBI42_002645"
     CDS             86..913
                     /locus_tag="NBI42_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HCD1778994.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            932..1324
                     /locus_tag="NBI42_002646"
     CDS             932..1324
                     /locus_tag="NBI42_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1778995.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            1358..>1926
                     /gene="pgsA"
                     /locus_tag="NBI42_002647"
     CDS             1358..>1926
                     /gene="pgsA"
                     /locus_tag="NBI42_002647"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCD1778996.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVF"
BASE COUNT          683 a          218 c          394 g          631 t
ORIGIN      
        1 gagcaaatgt ttttatgatt gatgttataa tatgaatatt acaatgaatc ataatttgtt
       61 aattaaacaa ggggtgaggt tcaccatgac agttgcagag aaaaaagaat ggtacctaga
      121 atacgaaatt gaaattaata gaccgggtct tttaggtgat gtatctagtt tattaggtat
      181 gttaggtata agtattgtta caattaatgg tgttgatcaa ggtaaacgag gccttttaat
      241 taaaacagac aatcttgaaa aagttgaacg ttttgagcaa atagctcgtg gtataaatga
      301 aattgaaata acaaagctta aaaaaccaga attaagagac cgtcttgcag ttagacatgg
      361 tagatatatt gagcaagatg caaaagataa gaaaaccttt cgatttgagc gtgaagattt
      421 aggcttgtta gtagactttt tagctgaatt gttcaaagaa gaaggtcata agttgattgg
      481 cattagaggt atgccacggg ttggtaaaac tgaatcaatt gttgcgggaa gtgtttgtgc
      541 acataagaga tggttattta ttagttctac tttaataaaa caaactgtac gtagctctct
      601 aatcaaaggg gaatatgatg ccaatcatgt atatattatt gatggtgcag ttactgccag
      661 agaatctaat ccaaaacatc aagagcttgt taacgaagtt atgacgttac catcaatcaa
      721 agtcgttgaa catccagatt tatttgttga aacaagtact tgtacaatgg aagattttga
      781 ctatattatc gaattgagag aaaatgaaaa tcaagaaata cattacgaag aaatgaagaa
      841 acagacagtc caaagtaaga ataatttaga ttttggagat ccatttggtg gtggttttgg
      901 tttcttcgag taagtgtaag gaggctatga attgaaaacg gtcggtgaag cgctaaaagg
      961 tagacgtgaa aggttaggaa tgactttaac agaattagag caacgtactg gaattaaacg
     1021 tgaaatgcta gtgcatattg aaaataatga attcgatcaa ctaccgaata aaaattacag
     1081 cgaaggattt attagaaaat atgcaagcgt agtaaatatt gaacctaacc aattaattca
     1141 agctcatcaa gatgaaattc catcgaacca agccgaatgg gacgaagtaa ttacagtttt
     1201 caataataat aaagacttag attataagag taaatcaaaa gagccaatac aattattagt
     1261 aatcatgggt attacagttt taataacttt attgttatgg atcatgttag ttttaatatt
     1321 ttaacagaaa taaattagtg agaaatgagg atgtataatg aatattccga accagattac
     1381 ggtttttaga gtagtgttaa taccagtttt tatattgttt gcgttagttg attttggatt
     1441 tggcaatgtg tcatttctag gaggatatga aataagaatt gagttattaa tcagtggttt
     1501 tatttttata ttggcttccc ttagcgattt tgttgatggt tatttagcta gaaaatggaa
     1561 tttagttaca aatatgggga aatttttgga tccattagcg gataaattat tagttgcaag
     1621 tgctttaatt gtacttgtgc aactaggact aacaaattct gtagtagcaa tcattattat
     1681 tgccagagaa tttgccgtaa ctggtttacg tttactacaa attgaacaag gattcgtaag
     1741 tgcagctggt caattaggta aaattaaaac agcagttact atggtagcaa ttacttggtt
     1801 gttattaggt gatccattgg caacattgat tggtttgtca ttaggacaaa ttttattata
     1861 cattggcgtt atttttacta tcttatctgg tattgaatac ttttataaag gtagagatgt
     1921 ttttaa
//
LOCUS       DAHHRR010000215         1925 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.220, whole genome shotgun sequence.
ACCESSION   DAHHRR010000215 DAHHRR010000000
VERSION     DAHHRR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.220"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            93..1415
                     /locus_tag="NBI42_002648"
     CDS             93..1415
                     /locus_tag="NBI42_002648"
                     /inference="COORDINATES: protein motif:HMM:NF024700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1778997.1"
                     /translation="MEVIGGVKIGTKESFTSTTEDDILNNQENIFSLGTHTDYYSNLY
                     KLGNNEKLYILERLNDIAHNLELFQAVQDYRVTQVSLMRGKFHKTVREQFHRIIKGQA
                     VLTSYEFQFKKEDIKIDFQVNPNSNPPSNLHGIIGSNGVGKTRLLKDILNGFLDSQAN
                     YIINKDSKNSFYDDNIFTNALFISFSIFDTLEINQTQNKNFSYIGVKKNIDENGSKRN
                     SNKNNDELADEFVNSIREIYNRNDESSERWSRFIEVLGLKNLFFELNKSEDNKKSFKR
                     QFNKLSSGQKIVVLSITKMVERVVEKTLILLDEPELYLHPPLLASYMKVISTLLTEQN
                     GVGIISTHSPVVMQELGKSNVNMIKSSDDNIYITKPRLETYGENLGTLTREVFGFEIE
                     NTGYYETLVNLVNEGYEYDDIIEKYKNSLGIEARSILRTLLKHKRIEQ"
     gene            1412..>1925
                     /locus_tag="NBI42_002649"
     CDS             1412..>1925
                     /locus_tag="NBI42_002649"
                     /inference="COORDINATES: protein motif:HMM:NF013964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1778998.1"
                     /translation="MIRLKSPQIELKDVIQDCIYNFRDSNKKESIEKIIPEIEKFHDK
                     YREYASKNELHLFKDPFASNNDLNNILVGLYTNKLSKINEKARNYYNLIMNYTTETND
                     VVIFSDEFKICPLCQTTEVNTLDHYLPKTKFPSLAVSPLNLIPICSKCNKNKSDYFSE
                     NAHSNLLHIYF"
BASE COUNT          793 a          204 c          304 g          624 t
ORIGIN      
        1 ctttagataa gggattttat ttgattaaac ataactggaa tgactacgga tataaaacga
       61 gatttgattt atattattat gacggtgaac atatggaagt gattggtgga gtaaagattg
      121 gtactaaaga aagttttact tccacaaccg aagatgacat tctaaataat caagaaaata
      181 tttttagttt aggaacgcat acagattatt attctaattt atataaatta ggaaacaatg
      241 aaaaattata tatattggag agattgaatg atattgctca taatctagaa ttatttcaag
      301 ctgtacaaga ttatagagta actcaagtct ctttgatgag aggaaaattt cacaaaactg
      361 ttcgggaaca gtttcataga ataatcaaag gacaagcagt tctaacttct tatgaatttc
      421 aatttaaaaa agaggatata aaaatagatt ttcaagtaaa tccaaattca aatcctccta
      481 gtaacttgca tggaattata ggtagtaatg gcgtgggaaa gactcgatta ttaaaagata
      541 ttttaaatgg gtttttagat agtcaagcaa actatattat aaacaaagat agtaaaaatt
      601 cgttttatga tgataacatt tttactaacg ctttattcat ttcgtttagt atttttgata
      661 ctttagaaat aaatcaaact caaaataaaa actttagtta tataggtgtt aaaaaaaata
      721 tagatgagaa tggatcgaaa agaaattcaa ataaaaataa tgacgaatta gctgatgaat
      781 ttgtaaatag tataagggaa atatataaca ggaatgatga atctagtgaa cgatggagta
      841 gatttattga agtactgggg ttaaaaaatt tgttttttga acttaataag agtgaagata
      901 ataaaaagtc attcaaaaga caattcaata aattaagttc tggacaaaaa attgtagttt
      961 taagcattac gaaaatggtt gaaagagtag tagaaaagac tttgatttta ttagatgaac
     1021 cagagttata tttacaccct cctcttttag cttcttacat gaaagttata tcaacattgt
     1081 taactgaaca gaatggtgtt ggtatcatat ctactcattc tccggtagta atgcaagaat
     1141 taggaaaaag taacgtcaac atgataaaat caagtgatga taatatatat ataacgaaac
     1201 cacgtttaga aacgtatgga gaaaacttag gtaccttaac tagagaagtt tttggatttg
     1261 aaatagagaa cacagggtac tacgaaacat tagttaattt agtgaatgaa ggatacgaat
     1321 atgatgatat tatagaaaaa tacaaaaatt ctttaggtat tgaggctaga tcaattttga
     1381 gaactttatt aaaacataag aggattgaac aatgattaga ctgaaaagcc cgcaaattga
     1441 gttaaaagat gtaattcaag attgcattta taattttaga gattcaaaca aaaaagagtc
     1501 aattgaaaaa atcatacctg aaatagaaaa gtttcatgat aaatatagag aatatgcatc
     1561 taaaaatgaa ttacaccttt ttaaagatcc ttttgcttca aataatgatt taaataatat
     1621 tttagttggc ttatatacta acaaattatc gaaaattaat gaaaaagcaa gaaactatta
     1681 taatcttatt atgaattata ctactgaaac taatgatgta gttattttta gtgatgaatt
     1741 taaaatatgt ccattatgtc aaacaactga agttaatact ttagaccatt atttaccaaa
     1801 aactaaattt ccaagtctag cagtaagccc attaaatttg ataccaatat gcagtaaatg
     1861 taataaaaat aaatcagatt atttttctga aaacgctcat agtaatttat tgcacatata
     1921 ttttg
//
LOCUS       DAHHRR010000216         1915 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.221, whole genome shotgun sequence.
ACCESSION   DAHHRR010000216 DAHHRR010000000
VERSION     DAHHRR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.221"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            305..619
                     /locus_tag="NBI42_002650"
     CDS             305..619
                     /locus_tag="NBI42_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD1778999.1"
                     /translation="MTYKEVATYLKVLSDSSRLEILDLLSCGELCACDLLVHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNFKTG
                     EC"
     gene            619..1911
                     /gene="arsB"
                     /locus_tag="NBI42_002651"
     CDS             619..1911
                     /gene="arsB"
                     /locus_tag="NBI42_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCD1779000.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMFIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKPPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKEV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLLGLYLTLIIF"
BASE COUNT          614 a          291 c          297 g          713 t
ORIGIN      
        1 taatctcatt agtaattcta tttatgattt ttagtcgtaa aaacaaagct tcttaatcaa
       61 tacccttgat tacaaaataa attcaactaa ggtgattggc catacactca agatatggct
      121 aatcaccttt ttcaatgcaa aacaaagagt ataatttgca aaatcaacat ctatatttat
      181 ctttaatacc cttttaatac taaaactaaa tttaaattac atagtcatat cgtgtaatat
      241 accaaaggaa catagacatt tatctatata gatgataatc tacttaacgt caaggaggta
      301 tgttatgacg tataaagaag tagcaacata tttaaaagtt ttatcagatt caagcagatt
      361 agaaatacta gatttacttt cttgtggaga gttatgcgct tgtgatttgt tagtacactt
      421 tcaattctct caacctacac ttagctatca tatgaaagca ttagtaaaaa ccaacttagt
      481 tacgacacga aagatcggaa ataaacattt ataccagctt aatcataata tttttgagtc
      541 cgtaattaat aacttgtcta aggttcatac ctctaaccaa cgatgtattt gtcataactt
      601 taagactggt gaatgctaat gatgacaact ttagcgacac tcatttttct agtaacttta
      661 ttatttgtat tatggcaacc taaaggccta gatattggca ttaccgcatt aactggtgcc
      721 tttattgctg ttattactgg tgttgtaagt ttttccgatg ttttcgaagt aacaggtatt
      781 gtttggaatg ctactttgac ttttgtctca gtcattctta tttcattaat attagataaa
      841 gttggattat tcgaatggtc agctattcac atgcttcatg cctcaaaagg caatggttta
      901 aaaatgttcg tttatatcat attattaggt gccattgttg ctgcattttt cgcaaatgat
      961 ggcgcagcgt taatcttaac gcctattgta ttagcgatgg ttaaaaatat aggttttagt
     1021 aagcgggcca tattcccttt tattattgcg agtggtttta ttgctgatac aacgtcttta
     1081 cctttaattg taagtaatct agtgaatatt atatctgccg attattttca tgtaggattc
     1141 gttcgatatt ttagtagaat gtttatacct aatttattct cacttttagc aagtattata
     1201 gtattgtggt tatattttag aaaagcgata cctaaaacgt ttgatgataa taatataaag
     1261 cctcctaaag atgctattaa tgatttaaag ctatttaaaa tttcatggat tgtgctagtt
     1321 atattacttt tcggctatct aatcagtgaa tttactaaaa ttccggtatc aattttcact
     1381 ggaatcattg cttttatatt tctaatgttg gctcgtaaat caaatgctgt aaatattaag
     1441 gaagtcatta agggcgcacc ttggaatata gtattatttt caattggtat gtatatcgtt
     1501 gtattcggct taagaaatgc tggcattact ttaatattgg ctaaaatatt agaatatatt
     1561 tccaattacg gtctatttag caccattttg ggaatgggct tcatttcagc atttttatca
     1621 tcaataatga ataatatgcc tacagtttta atagatgcga ttgctattgg tcaatcaaat
     1681 gtccatggca tgttaaaaga aggcctaatt tatgcgaatg ttatcggttc tgatttaggt
     1741 ccaaaaatta caccgatagg ctctttagct acattactgt ggttacacgt cttaacacaa
     1801 aaagatgtta agatttcttg gggcacatac tttaaaactg gtatcatcat tacaattcca
     1861 gtactattta taaccctctt aggattgtat ctaacactta tcatatttta agaaa
//
LOCUS       DAHHRR010000217         1861 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.222, whole genome shotgun sequence.
ACCESSION   DAHHRR010000217 DAHHRR010000000
VERSION     DAHHRR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.222"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            217..981
                     /locus_tag="NBI42_002652"
     CDS             217..981
                     /locus_tag="NBI42_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCD1779001.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYGLQQRYPNISFAHHHGYIDLEDETVVKRIEL
                     FKPDYIFVGMGFPKQEEWIMTHENQFKSTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WVYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAR"
     gene            complement(1042..1836)
                     /gene="tagH"
                     /locus_tag="NBI42_002653"
     CDS             complement(1042..1836)
                     /gene="tagH"
                     /locus_tag="NBI42_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HCD1779002.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
BASE COUNT          637 a          292 c          317 g          615 t
ORIGIN      
        1 agtaatttaa agtttgctta tcacttatta taaatgatat tggcatcaat agcgttagac
       61 tttagactta ccttagttaa actaatttta atttttgaaa aggtgaatat gtgttaaaat
      121 aaagcaaaat catttcgata taaataggat gaatataaat actgttaata ttgattacac
      181 taacataata atgaaataag ataggagatt cctgttatga ctgttgaaga aagatccaat
      241 acagccaaag ttgacatttt aggggtcgat tttgataata caacaatgtt gcaaatggtt
      301 gaaaatatta aaaccttttt tgcaaatcaa tcaacgaata atctttttat agtaacagcc
      361 aaccctgaaa tagtgaatta cgcgacgaca catcaagcgt atttagagtt aataaatcaa
      421 gcgagctata ttgttgctga tgggacagga gtagtcaaag cttcgcatcg tttaaagcaa
      481 cctctagcgc atcgtatacc tggtattgag ttgatggatg aatgtttgaa aattgctcat
      541 gtaaatcatc aaaaagtatt tttgctaggg gcaactaatg aagttgtaga agcggcacaa
      601 tatggattgc aacaaagata tccaaacata tcgtttgcac atcatcacgg ttatattgat
      661 ttagaagatg agacggtagt gaaacgaatt gaattgttta aacctgatta catatttgta
      721 ggtatgggat tccctaaaca agaagaatgg attatgacac atgaaaatca atttaaatct
      781 acagtgatga tgggcgtagg tggttctctt gaagtatttg ctggggctaa aaagagagcg
      841 ccttatatct ttagaaaatt aaacattgaa tgggtatata gagcattaat agattggaaa
      901 cgtattggta gattgaagag tattccaata tttatgtata aaatagccaa agcaaaaaga
      961 aaaataaaaa aagcgagata gtcatgatga caaaaataaa accgaggaaa tccttaaatg
     1021 gagattctcg gttttttcgg tttatttaat aacgaagcgg gactcatcga gtttgtttct
     1081 aaattctttt tgttcggctt tggatttctt tttaaaatcg tttaggaaag cttcatattt
     1141 aggtaataca tcatcaagtt caccgtaatc ttttaacttt ccgccttcaa tccaagcaat
     1201 cttagtacaa aattgtctca cttggcctaa gttatgacta acgaaaaaga tggttttgtt
     1261 ttgctcttta aactcgtaaa ttttatctaa acatttttgt gcaaaagttt ggtcacctac
     1321 agataaagct tcgtcaatga ctaagatatc tggattaact gtgatattaa ttgaaaaacc
     1381 aagttttgca cgcataccac ttgaatactt tttaactggt tgataaataa actcaccaag
     1441 ttcactaaat tcaataatct taggtgtcat cgctttaatt tctttacgct taaagcccat
     1501 acataacatt ttaaattcga tattttcaat ccctgtaagt tgtccactca agccagcact
     1561 aattgcgata acgctgactt caccattacg atccacttta ccaacagtag gcgacaaaga
     1621 accgccaatg atattgctca acgttgattt gccggaacca ttgatgccaa caagccctat
     1681 gacgtcacct tcatatgctt ttaaactaat gtcatctaaa gcgaaaaatg ttttgttttt
     1741 atgtttggga atgagcgcat ctttcatacg ttctttattt gtacgataaa tacgatattc
     1801 ttttgttaca tttttaatgt ttaccgaaac gttcatttgt agaccttcct tattcacatt
     1861 t
//
LOCUS       DAHHRR010000218         1857 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.223, whole genome shotgun sequence.
ACCESSION   DAHHRR010000218 DAHHRR010000000
VERSION     DAHHRR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.223"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..365)
                     /locus_tag="NBI42_002654"
     CDS             complement(<1..365)
                     /locus_tag="NBI42_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1779003.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHKMIH
                     RNTKKPLTIKELTKIVNYN"
     gene            complement(362..499)
                     /locus_tag="NBI42_002655"
     CDS             complement(362..499)
                     /locus_tag="NBI42_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779004.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            555..1857
                     /gene="cstB"
                     /locus_tag="NBI42_002656"
                     /pseudo
     CDS             555..1857
                     /gene="cstB"
                     /locus_tag="NBI42_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
BASE COUNT          631 a          303 c          280 g          643 t
ORIGIN      
        1 ctattataat taactatttt ggttaattct ttaatcgtta aaggtttttt agtatttcta
       61 tgaatcattt tatggcaatt agaacatagt gggactaaat ctttttgtgg atttactttt
      121 atttctcttt ttattgaaaa cattggtttt aaatgatgaa tttcaataaa accctcacct
      181 aaatcgccat acttagcttt aaaatcaaat ccacatactt tacatgtagt tccatgaatt
      241 ttaatagctt catttcttaa tgcctgattt ctttcatatt taataccgta ctgtacatat
      301 ggtttacctt cgacatattc agtgttttct aactcattat ctaaatttaa aatattcgtt
      361 ttcaagactt ttgtatataa ctttttaaag gttcctttaa agtcatagga ccttgaacat
      421 taccttttaa cccattatct tgtaaaaatt ctaaatctaa ttggtcatca tcaagtttgt
      481 ttaataactt taattccatg taattaccat agttaatata attatcaccc ttgataacaa
      541 attgactatc atcaatttat gataaacatt tatctcaagc atcttattta atcggttgtc
      601 aaaaaactgg agaagccatg atcattgatc ctatttgtga cttatcttca tatattcgag
      661 ttgccgatga agagagttta accattactc atgcagctga aacacatata catgcaaatt
      721 ttgtttcagg aattagagat gttgctataa agttaaatgc tagtatttat gtatcgggcg
      781 aaagtgatga catgttaggt tataaaaata tgcctaacca cactcatttt gttcaacata
      841 atgatgatat ttatgtagga aatataaaat taaaagtgct tcatacacct ggtcacacgc
      901 cagaaagtat aagtttttta cttactgacg aaggtgctgg agcacaagtt ccaatgggac
      961 tattcagtgg tgattttatt tttgtaggag atatcggtag acctgattta ctagaaaaag
     1021 ctgttaaagt agaaggatta tctgaaatag gcgcaaaaca aatgtttaaa tctattgaaa
     1081 gtattaaaga cttgccagac tacattcaaa tttggcctgg tcatggcgct ggtagtcctt
     1141 gtggtaaatc tttaggtgct attccaacat ctacgcttgg ctatgaaaaa caaacgaact
     1201 gggctttttc tgaaaataat gaagctacct ttatcgataa actcatttct gaccaacctg
     1261 caccaccaca tcattttgca caaatgaaaa aaattaatca attcggtatg aatttatatc
     1321 aaccttatac ggtttatcca gctacaagta caaacagatt aacttttgat ctccgcagta
     1381 aggaggctta tcatggtgga catattgaag gtacaatcaa tattccatat gataaaaatt
     1441 tcatcaatca aattggctgg tatctaaact ataatcaaga aattaacttg attggagact
     1501 atcaccttgt ttcaaaagca acacataaca tactttacaa cttattggat atgataatgt
     1561 gtctggatat cagttaccac aagctcaaat tcaaacacaa tctgttcata gtaaagacat
     1621 aactggtaat gaagcacatg tcttagatgt acgtaatgat aatgaatgga ataatggcca
     1681 cttatcccaa gcggttcatg tgccacacgg caaactttta gaaacagatt tacctttcaa
     1741 tagaaacgat gttatttatg tagactgtca gtctggcatt agaagttcta tagctattgg
     1801 tattttagaa cacaaaggtt atcacaacat tattaatgta aacgaaggtt acaaaga
//
LOCUS       DAHHRR010000219         1811 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.224, whole genome shotgun sequence.
ACCESSION   DAHHRR010000219 DAHHRR010000000
VERSION     DAHHRR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.224"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..149
                     /locus_tag="NBI42_002657"
     CDS             <1..149
                     /locus_tag="NBI42_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCD1779005.1"
                     /translation="PSIADEKNFIFLSILINLGVDALLVRSYFNYALYKSIKKDIENE
                     GKTM"
     gene            183..771
                     /locus_tag="NBI42_002658"
                     /pseudo
     CDS             183..771
                     /locus_tag="NBI42_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
     gene            complement(911..1804)
                     /locus_tag="NBI42_002659"
     CDS             complement(911..1804)
                     /locus_tag="NBI42_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1779006.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNNSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRQPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
BASE COUNT          534 a          243 c          347 g          687 t
ORIGIN      
        1 cgcctagtat tgcggatgaa aagaacttta tatttttaag tatactaata aatttaggtg
       61 ttgatgctct acttgttagg tcatatttta attacgcgtt atacaagagt attaagaaag
      121 atatcgaaaa tgaaggtaag acaatgtaaa ggaaagacat aaaaaataat aggagtctaa
      181 ccatgataat ttttatttta ataacaatat ttgctattta ttatatagct atgattgcta
      241 gtttgtttaa aagtgaaggt ttttcaataa taggtttaat attagatgtt gttatattgg
      301 ggacgttgat tttttactat tttataggcg ctcgctttgt tgataatgat ttaagcaatt
      361 ttttagcatt tatgcatttg ggttcatttg tgtatatgta ctttgcaatt aagtgtttat
      421 gggtgaaacc taaggtagta aattatttga ttgcaaaaga attggatgaa tctaaagagg
      481 tcattgaaga gcaggaatta gatttacaga catcaaagat aagaggtatc tactttttca
      541 ttattgcagt agtgatgtta attatcgcta aattaaggat gcaaccagaa ttacaagcag
      601 atgcggtatc aatgaatcca gtttttaatt tttataggtg ttattatcat tttaatttgg
      661 ctaatactag atatatatcg taagaagaaa tacggtatat tcttattcaa gacgatagtg
      721 ccattagttg ttacgacttg gataattata gctacaatta tactttcgta aatgattttg
      781 attaatgggg aatgtagaga gcatttcttt gtaactttat aatcgaatag catacaaaat
      841 aaagcgacca gctaacattt tacatctatt aaaatgttgt ctggtcgctt tttattaatg
      901 tcattattaa ttagtgaatg taattatatg atgaaacttc tgaagcagag atggttcttg
      961 atgaaacgat atattcacca atccaattca tttctgaaat tagaatactt ccatcaatat
     1021 taactttttc aacgtaggct acatgaccaa atggaccgtt tactgtttgt aaaattgatc
     1081 ctcgtgttgg ttgtctatct actttgaagc cattgcttga agcttggcct gcccagtttt
     1141 tagcatctcc ccaaaatgta ctaatcgtgt gtccatcttt ggcgcgttta tcaaagacat
     1201 accatgtaca ttgtccagca gtatataagt tgttcttact tgtgataaga ggctgatcaa
     1261 tgattttacc gttacctaaa gctaaaggtt taccgtcagc agtctttgct ttgtcattaa
     1321 attcggcgat ttgtaattcg tcatacaatt cgtctaaatc gatgcctgta ataagtcctt
     1381 tgttatcttt tgaaaaagcg ttatttaatt catcgtcatt gtcttcgaca ttcggtattg
     1441 ctggtgtcaa aggattgctt ggtgacgttt gaggcggtgt gtgtgaatca attgcgtcat
     1501 taatgtgcgt atactgacca cttaatgaag aatggtactg attgttgtta agatcacgtt
     1561 gatttgcgtg ttgatgattg tcgtttgtac gtgactggtt ttgatgattg ttgtttggcg
     1621 tgttgttttt gtcatatgta taagtatacg cgccggtgtc tttattcact ttgaactgtg
     1681 cgtttgggtg tgctttcttt gcttcttcta atgttttgct attattcgta tatgcttgag
     1741 ccgagttagg cgacatacta aataaagtaa gagttgtcat cgtcagtaaa attgttttct
     1801 tcataataac c
//
LOCUS       DAHHRR010000220         1797 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.225, whole genome shotgun sequence.
ACCESSION   DAHHRR010000220 DAHHRR010000000
VERSION     DAHHRR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.225"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..298
                     /locus_tag="NBI42_002660"
     CDS             <1..298
                     /locus_tag="NBI42_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1779007.1"
                     /translation="YQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLIN
                     GEHDPLIKDKNHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFLSYYLNFLKSVS"
     gene            complement(551..925)
                     /locus_tag="NBI42_002661"
     CDS             complement(551..925)
                     /locus_tag="NBI42_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCD1779008.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
BASE COUNT          647 a          233 c          236 g          681 t
ORIGIN      
        1 gtatcaaaaa caaagaatat atagcattaa gtctgaggat gatagtgcgg tggcaattcg
       61 gtcattaatt ttgcataaag atgaacctat gtatttaaaa aaacgtactt gtgtacctac
      121 tttgttaatt aatggggaac atgatccttt gattaaagat aagaatcatt ttaaattgga
      181 agcgcatttt ttaaatgtaa cgaaaaaaat cttcgaacat tcaggacatg caccgcatat
      241 tgaagaacca gaagcatttc taagttatta tctaaatttc ttaaaaagcg tatcataata
      301 tttgatatat aaacctaggg cataaagtcc ttaggcaatg tgaaaaagct gattactatt
      361 cattatttga tagaaatcag ctttttttga aatgtatttg atatatatac tgctcgttaa
      421 gcggctatct tccttatatt aagtgccatt agtgcaaaac ctcttaacaa ttaggtaaaa
      481 agagcataaa aaaaggaagt ttaatagaat gtatcatcta tcaaacttca ccaaattgcg
      541 ctaaacaaaa ttatagttca atttcgttgt ttgcttcagt gattcgttta tttactcgac
      601 tcaataatga ttcgattttt ttacgttgtt gtgcattaac aagaattaat acagttcttt
      661 catcatgctc attacgtttt ttatcgaagt aatcttcttg agataaaatt ttaactgctt
      721 taacaacttg tggttgtttg tagtttaaat gattaataat atctttaaga tagtattctt
      781 tctctttgtt ttcgctgatg tatgtcaata cagcgaattc ttcaaagcta attgaaaatt
      841 cctttttaat taaacttttt aatttgtcag cataagtgac cattgataac aactcaaagc
      901 aatcattgat ttttgtaatt gccatgttta aaacctccct atttgatgca tcttgctcga
      961 tacatttgcc cgataatata ttgatatcta atctttattt attatagata tgttagtcat
     1021 aattttgcat taaataagtt ttattaaata tatttaatgc tctattattt agttaattat
     1081 aactaattaa aaatgagaag taaacaaaaa agtgtttata aaacaaatta ttcattagac
     1141 acagtgattg tatttctggg ttagcatttg gtttagtcaa aaatatcagc attttttata
     1201 atttacctta atttaatcgt caatacaagg taatttattt aatgaaacga tttagcgcaa
     1261 ttaaatgttt cgatattcaa tgtgtatcta aagtgattct ttgtcatagc ttaacgcttt
     1321 caagttaact tcttctaata tttataccca tttttcgaac ttttatcact aaaaaagaaa
     1381 aatttttata atttttttaa tttagtaatt cccagtgcag ttttaatgat aaggaaaata
     1441 cactatttta atgacaaaag taaaactata gcactttaaa gtaaatgtat agtaatccga
     1501 ttgataacaa gtaatcaata ttagaaagta cgattatggt tcataattta aagcatcttc
     1561 aatctttaaa gtcgactcaa tgaatgaatt aaagttgtac attttgactt atttttaatt
     1621 aattgaataa ccaactatga aatattagca cgaataacat aatttttgct attaatttgg
     1681 tataaattat taaaaaacaa cattaattta ggtcttaaga atgagttgaa tgcctaattt
     1741 gctaaattaa aatttgttgt gtcaaagcat actacccata ttaatactta ttaaaac
//
LOCUS       DAHHRR010000221         1746 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.226, whole genome shotgun sequence.
ACCESSION   DAHHRR010000221 DAHHRR010000000
VERSION     DAHHRR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.226"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..502)
                     /locus_tag="NBI42_002662"
     CDS             complement(<1..502)
                     /locus_tag="NBI42_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCD1779009.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEE
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRL"
     gene            complement(562..1086)
                     /locus_tag="NBI42_002663"
     CDS             complement(562..1086)
                     /locus_tag="NBI42_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779010.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            1207..>1746
                     /locus_tag="NBI42_002664"
     CDS             1207..>1746
                     /locus_tag="NBI42_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1779011.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSI"
BASE COUNT          639 a          293 c          223 g          591 t
ORIGIN      
        1 ttaaacgata aatgtattct aaaatcttaa atatgacaat gataaatact aatcctaatc
       61 ctgaatatga tatggcaaaa aatacattac taattgagtc tacaattcct ctggatattg
      121 ctgatgctaa taaaataatt ggacaaaaca gcagcaaaag tgatattaaa aataatgcta
      181 atacaaataa tccaactata gatactggta taaggatgac aaaaatagat agaatgccta
      241 aacttaatga agcagcaaca gctcttgaaa tattttcaaa tgttggttta gattcagcat
      301 aatcaataac acttctagct tttgtttcag ctgcaacatc ttgtgggtct tttaacttct
      361 ttaaaatttc ctcttcatca acgccattaa gttcttgctc ataaaaataa cgttcatatt
      421 catacatttt ttgatctctt aaatgcctag gtagcgcatc aagtgctgtt tctaattcat
      481 ttaaataagt aatcttgttc atatcggtca ctcatcccaa taaaattatt cattcgtttt
      541 ttataacttt aagacctaaa actatttact aaaaaattca atacgtttaa tttctaaatc
      601 ttgataaagt aagtttaatt ttaaatggtt atctctatca atatacttta ccgatgagaa
      661 atcattgcca acttttgttc ttttatatcc gaatccaaga ttttcaatcg cgttatttat
      721 cggcgaacca attcccccat caaaattagt atgtatcttt tcatctctta ctaatattat
      781 ccccttaacg atgttatcct tttctctcac tttaacggta aggtcagggt gagctttact
      841 gttataaaat ttaaaacgat caaacttaat ttttttattt acttcatagc cttttttatc
      901 aaacttctca tttaatttaa tatcgtttat ttcaagatta gacaaatcca cagttttcgt
      961 atgacgatat tgatataaac caattaatac accaaaagaa cagattaaaa ataatataaa
     1021 cacaacccag cgattttgaa tacgcactga tgtcaccctt tctttgtact tatttaaata
     1081 tagcataagg tgcttcccgt ttcatcctta acaaaaatta acaaagaatt tgcaattaat
     1141 ttatttcatt accttttatt actataaatt ggtataatgt tgtaaatgct aaaggaggtt
     1201 tttacaatga caaatttaga aacttatttc aaaaatagcc aacccttaaa tgaatacatt
     1261 gatggaatga agacaaatca agataccgtt ctcagtatct atcaatcatt tgagttacca
     1321 gaagatgatt cacgcatcaa aaaaataaaa gagatgaatt attctaaagt attagtaatt
     1381 actgaagact ggtgtggaga tgcaatgatg aatctcccaa ttctaaaaca catcagtgaa
     1441 gcattaaatt tagaagtacg cgtgttccat agagatgatg atacaaaact gatcgatcaa
     1501 tatttaacaa atggtaaatc acgtgcaatt cctatcttcg tattcttgaa tgatcaattc
     1561 gaacaagaaa cagtttgggg accaagagca aatgaagtcc aaaaatttgt aactgatgtt
     1621 cgtgctgata aattaccgag caaagatcat ccagactatg cagatttaga aaaagaaaca
     1681 cacttagtta tatcaaaccg ctataaaact gacgcttcat tttggaaagc agtttataat
     1741 tccatc
//
LOCUS       DAHHRR010000222         1746 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.227, whole genome shotgun sequence.
ACCESSION   DAHHRR010000222 DAHHRR010000000
VERSION     DAHHRR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.227"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(65..973)
                     /gene="rocF"
                     /locus_tag="NBI42_002665"
     CDS             complement(65..973)
                     /gene="rocF"
                     /locus_tag="NBI42_002665"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HCD1779012.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKVVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSIDLVEVN
                     PLIDRNNHTAEQAVSLVGTFFGETLL"
     gene            complement(1105..1177)
                     /locus_tag="NBI42_002666"
     tRNA            complement(1105..1177)
                     /locus_tag="NBI42_002666"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1142..1144),aa:Lys,seq:ttt)
     gene            complement(1181..1255)
                     /locus_tag="NBI42_002667"
     tRNA            complement(1181..1255)
                     /locus_tag="NBI42_002667"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1221..1223),aa:Gln,seq:ttg)
     gene            complement(1265..1348)
                     /locus_tag="NBI42_002668"
     tRNA            complement(1265..1348)
                     /locus_tag="NBI42_002668"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1312..1314),aa:Tyr,seq:gta)
     gene            complement(1378..1453)
                     /locus_tag="NBI42_002669"
     tRNA            complement(1378..1453)
                     /locus_tag="NBI42_002669"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1418..1420),aa:Val,seq:tac)
     gene            complement(1461..1532)
                     /locus_tag="NBI42_002670"
     tRNA            complement(1461..1532)
                     /locus_tag="NBI42_002670"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1497..1499),aa:Glu,seq:ttc)
     gene            complement(1534..1608)
                     /locus_tag="NBI42_002671"
     tRNA            complement(1534..1608)
                     /locus_tag="NBI42_002671"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1574..1576),aa:Asn,seq:gtt)
     gene            complement(1620..1734)
                     /gene="rrf"
                     /locus_tag="NBI42_002672"
     rRNA            complement(1620..1734)
                     /gene="rrf"
                     /locus_tag="NBI42_002672"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          532 a          376 c          293 g          545 t
ORIGIN      
        1 tttagtgaat taaaaaaagt agtgcaaaaa gcaaaatata ctttatacac tacaaatcat
       61 ttatttatag taaagtttca ccaaaaaatg ttccaactaa cgaaaccgct tgttcagcag
      121 tatgattatt tctgtcaatc aatggattta cttcaactaa atctattgag gaaattaaat
      181 gtgattgatg cagtaattcc aatgcaaaat ggctttctct ataactaaga ccacccaaaa
      241 ctctagtacc agtgcctggc gtttcaagcg gatctaaagc atcaacatct aaagataaat
      301 gaacgccatc aacattgcgt gacttcaaat attctattgt attttcaatt acttccttta
      361 tccccaattt atcaatatct gacattgtaa atgttttaat attatgatct ttgataaatt
      421 gtctttcacc tttatctaaa tctctcatac caattagtac gatgttttct ggcttgatta
      481 cattactatt taattctaaa agttctttgg gaccttcgcc tgtcaaaatc cttagaggca
      541 taccatgaat atttccactt ggtgactctt caggtatatt taaatcacca tgtgcatcat
      601 accaaataac acctaaattg ttataatgtt tacttattgc tgatactgaa cctaccgcaa
      661 tagaatgatc tccaccaaga actagaggaa atctgttatt ttcaattgat gctgaaacta
      721 ctttatttaa tttttgatta acatctataa tttcatcata atttcttaat cctttttgtt
      781 cactatgaaa tttttcaatg ttcacagcag gtaccttaat atcccccttg tcatatacat
      841 caaggtctaa ttgctttaat cttgaaatta atccagcata tctaattgct gttggtccta
      901 aatcaacacc taattttctt tgtccaaatg ttgatggtgc acctataata tcaattgctt
      961 ttgtctttgt cataagcgtc cccctttgct ctatgtaatt aaagaataat gtatgcgctt
     1021 accattatca agcaatagct acacatataa cctgtttatt acttcataaa aaaagcactt
     1081 cccaaaaatg gaaagtgcaa gtagtgagcc atagaggatt cgaacctctg accctctgat
     1141 taaaagtcag atgctctacc aactgagcta atggctctaa tggctgggct agctggattc
     1201 gaaccaacga gtgacggagt caaagtccgt tgccttaccg cttggctata gcccaatata
     1261 tagatggtgg aggggggcag attcgaactg ccgaacccga aggagcggat ttacagtccg
     1321 ccgcgtttag ccacttcgct acccctccag cttattcata taatttaata atcaaaatgg
     1381 tggagaatga cgggttcgaa ccgccgaccc tctgcttgta aggcagatgc tctcccagct
     1441 gagctaattc tccaaaataa tgactcctac gggactcgaa cccgtgttac cgccgtgaaa
     1501 gggcggtgtc ttaaccgctt gaccaaggag ccatggctcc acaggtagga ctcgaaccta
     1561 cgaccgatcg gttaacagcc gatagctcta ccactgagct actgtggatt aatattaatg
     1621 cctggcaacg ttctactcta gcggaacgta agttcgacta ccatcgacgc taaggagctt
     1681 aacttctgtg ttcggcatgg gaacaggtgt gacctccttg ctatagtcac cagacataga
     1741 atgtaa
//
LOCUS       DAHHRR010000223         1726 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.228, whole genome shotgun sequence.
ACCESSION   DAHHRR010000223 DAHHRR010000000
VERSION     DAHHRR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.228"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            84..1538
                     /locus_tag="NBI42_002673"
     CDS             84..1538
                     /locus_tag="NBI42_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HCD1779013.1"
                     /translation="MNKEDYLKNIVSEKISNGAEDSTTEFKVNNDDPKTIGEYISALG
                     NSATLEDKDYAYMIWGIEDESKKVVGTKFHPNNAKKGQEDLQNWLHRMIKPSIDFTFD
                     TIDYDGKNITILRIPKAIHQPISFNGEEFIRVGSYKKKLRDYPEKEKQLWKKFQELPF
                     ESLSAYLVKNYDEIFELLDYESYYKLLKQPLPIQKDLIIDHFKHEEIIKENEGNVYIT
                     NLGAILLAKNLDNFKPLKRKAPRVIKYKGNNKIETISEQVGTKGYACGFEGLIKYINN
                     LLPSNEVIGEVFRETVQMYPELAIRELVANSLIHQDLLISGAGPVIEIFDNRIEIVNP
                     GTPLIKVERFLDSPPKSRNESLASLLRRMGICEERGTGIDKVISETEKYQLPAPLITL
                     YDEFIKVTLHAYKDINDMTREEKIRATYMHACLKYVESESITNQSLRKRFGLEDKKIS
                     VISRIIKDTVAEEYIKPVDPETSPRHMRYIPYWA"
BASE COUNT          682 a          245 c          269 g          530 t
ORIGIN      
        1 acatatatac ccatgttata gataaaataa caagaaaatg ataaataatt aaaaatagta
       61 atataagttt aggagttaat attatgaata aagaagatta tttaaaaaat attgtttccg
      121 aaaaaataag taacggcgct gaagattcaa ctactgaatt taaagtaaat aacgatgacc
      181 ctaaaacaat tggagaatat atctcagctt taggaaattc agcaacttta gaagacaaag
      241 actacgctta tatgatatgg ggaattgagg atgaatcaaa aaaggtggtc ggtaccaaat
      301 tccatcctaa taatgctaaa aaaggacaag aagacttaca aaattggctt cataggatga
      361 ttaaaccttc catagatttt acttttgata ctatagatta tgatgggaaa aatataacaa
      421 tattgagaat ccctaaagcc attcaccaac caatttcatt taacggtgaa gaatttataa
      481 gagtaggatc ctataagaaa aagctaaggg attatcctga aaaagaaaaa caactatgga
      541 agaaattcca agagttacct tttgaatcac tttctgcata tttagtaaaa aactatgatg
      601 aaatttttga attactagac tacgaatcgt attacaagct attaaaacaa cctcttccaa
      661 ttcaaaaaga tttaattatt gatcatttca aacatgagga aatcataaaa gaaaatgaag
      721 gtaatgttta tattacaaat ttaggagcga ttttattggc taaaaattta gataatttta
      781 aaccacttaa aagaaaagct cctcgagtta ttaagtataa aggtaataac aaaatcgaaa
      841 ctattagtga acaagtcggt actaaaggat atgcttgtgg atttgaagga cttattaagt
      901 atattaataa cttattacca agcaatgaag ttattggtga agtttttaga gaaacagttc
      961 aaatgtatcc agaattagcc ataagagaat tagtagccaa ttctcttata catcaagatt
     1021 tacttattag tggcgctgga cctgtgatag aaatatttga taatagaatt gaaatagtta
     1081 atccaggcac tccattaatt aaagttgaaa gatttttaga ttcgccacca aaatctagaa
     1141 atgaaagtct agcttcctta ttaagaagaa tgggcatttg tgaagaacgt ggaacaggaa
     1201 ttgacaaagt aatttcagaa actgaaaaat atcagctccc cgctccatta ataactttat
     1261 atgacgaatt tattaaagtt actctacacg cctacaaaga tattaatgac atgactagag
     1321 aggaaaaaat tcgtgcaaca tatatgcatg cttgcttgaa atatgttgag agtgaaagta
     1381 ttacaaatca atcactgcga aaaagatttg gattggaaga taaaaaaatc tcagttattt
     1441 caagaattat aaaagacaca gttgctgaag aatatatcaa acctgtagat ccggagactt
     1501 cacccagaca catgagatat ataccatact gggcatgatt ttttatttgc gctttacttg
     1561 cacgttattt tcgttaaaca acttcattat caattaaata cgacctaatc cttcttttta
     1621 aagggattta ggtcgctttt ttatttgcac tctacttgca ctttacttgc acgttatttt
     1681 cattgaacaa cttcattatc aattaaatac aatctaaccc ttgttt
//
LOCUS       DAHHRR010000224         1714 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.229, whole genome shotgun sequence.
ACCESSION   DAHHRR010000224 DAHHRR010000000
VERSION     DAHHRR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.229"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..192)
                     /locus_tag="NBI42_002674"
     CDS             complement(<1..192)
                     /locus_tag="NBI42_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779014.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKD"
     gene            417..998
                     /locus_tag="NBI42_002675"
     CDS             417..998
                     /locus_tag="NBI42_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD1779015.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1071..1688)
                     /locus_tag="NBI42_002676"
     CDS             complement(1071..1688)
                     /locus_tag="NBI42_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCD1779016.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGEAQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIVIIVALMILQKLIQLLF"
BASE COUNT          618 a          270 c          262 g          564 t
ORIGIN      
        1 atcttttgtt ttaaagttta tttcatcatt tcttatcgaa aatggtgtaa taatgtcttt
       61 atctaaccaa gtgtttataa gttcatttgg tacaccatct aacaacattt cacttttact
      121 aattataaaa cattcccagt caagtgaaac attttgtgga ttcacataat tacattgatt
      181 atgattatcc ataaacactc actcctttaa aattctgtac tcttcattgc gttttacccc
      241 gtcacattat cttttaaact aaaatcatca ttacttatga aaaaaatgta catcaaaagc
      301 aaaggttttc gctaccgaaa aagtttaaat aatggtttaa tatatttggt actcatttta
      361 ataaaaagag aatacatttt gagctatcaa tactttttat tgaagaggtg ttattgttgg
      421 ctaaaacgtt atatttaatg cgccacggac aaactttgtt taattttaag ggactaattc
      481 agggatttgg agattcgccg ctaacagaac ttggaattgc tcaagctcaa aaggcacgta
      541 gttattatga aactaagggg ataaacttcg atttatatgc atcatcaacg caagaacgcg
      601 caagtgacac acttgaaaat gttgcaccta accaatcgta tcaacgtttt aagggactga
      661 aggaatggca ttttggaata tttgaaggtg agtcagtcta tctatttgat aatctataca
      721 agcctgagga cttattcgga gatcgaattg ttcctttcaa aggagaggca aggcaacaag
      781 ttgaagaacg cattgtgaaa actttacatg acattatgtc tcaaacaaag aataatgcat
      841 tagtcgtgag tcatggaaca ataatgggag tatttttaag atattgcctt aaactagatg
      901 aagcattaaa gcataatatc ggtaattgta atatcctgaa atttgaatat gacaatggaa
      961 catttaaatt tgttgagtta attgatccaa atttataata aataaagcat tatacataga
     1021 cacaagtatg tcttttgtat aacgctttta aaatacaact acataacgct ttagaataat
     1081 aattgaataa gtttttgtaa tatcatcaga gcaacaataa taactattat gcttgatatt
     1141 ttattgatta ttgttaataa ttttccggtt ttatcaattg atccaaccat ttttcctaaa
     1201 attgcgagta aaaagaacca taaccatgaa acgctaatac aagcgattgt aaacgcaatt
     1261 ttattgctgc cactatataa tgcagcacta ctaccaatta caccaattgt atctaaaata
     1321 gcatgtggat tgagtaatga aactgataaa gcaaaactta cttgtttcat tggagacata
     1381 atttgagctt ctccatctgt tgagggttta tcatgccaaa tggtccaagc catatacatc
     1441 aagaaaatta aaccaactat ataaataatt gcttgaagta caggtaaaga cataataatg
     1501 atagatactc ctaccactgc aataataata agtaagctgt ctgacaaccc ggctgtaatt
     1561 attgcaggca atacatatct atattttggt tgattagctc cttggttaaa aataaataca
     1621 ttttgtgcac ctaaaggtaa aattagacca atcgctaaga taaatccatg aataattgcg
     1681 gttaccatta agttttgaca cccctaaaac atag
//
LOCUS       DAHHRR010000225         1702 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.230, whole genome shotgun sequence.
ACCESSION   DAHHRR010000225 DAHHRR010000000
VERSION     DAHHRR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.230"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..129)
                     /locus_tag="NBI42_002677"
     CDS             complement(<1..129)
                     /locus_tag="NBI42_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCD1779017.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFR"
     gene            complement(266..499)
                     /locus_tag="NBI42_002678"
     CDS             complement(266..499)
                     /locus_tag="NBI42_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779018.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANVSAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRGGKVFALIN"
     gene            642..1265
                     /gene="lexA"
                     /locus_tag="NBI42_002679"
     CDS             642..1265
                     /gene="lexA"
                     /locus_tag="NBI42_002679"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCD1779019.1"
                     /translation="MRELTKRQSEIYNYIKQVVQMKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
BASE COUNT          577 a          272 c          256 g          597 t
ORIGIN      
        1 tctaaaactc tcaagataag cttttcttaa ggctgtttgc tcctttgctt cttcttgagt
       61 taatcctact tcttttttct ttttagctaa ctcgttaatt ctttcgatat tcaaatcaga
      121 attactcaat taaaaaacct accttcaatt tgtttattca atcattataa tactttaaca
      181 aaatataaca aatttaagat cagcaaaaaa gtaataaaaa aacgctattt cgcaaaaata
      241 ggcaaaataa cgttgtgaca atgtatcaat ttattaaagc gaacactttc ccacctcttt
      301 gttcgctatt gtttgtaggg tctttatgtt cgtatgcttt atttatcgta ttgttcttaa
      361 tttgatggtc agtcatttcg tacacttgtt ccgagtgagc actaacgttg gcacttaaca
      421 agaatattgc aaaggctgca cttgaaacta aaaatatgat gatatatgtt gatacgtttt
      481 tatatttatt gtaaaacatc attttcactc ctagaacatt tgtttgtata agtaatttat
      541 cagaacttat gttctgtgtc aatattttaa cgaacaaatg tttgttttgg ttgtaaatta
      601 atgatatact gtatttaaat taatttaggg agtgcctata tatgagagaa ttaacaaaac
      661 gacaaagcga aatatataac tatattaaac aagttgttca aatgaaaggt tatccgccta
      721 gtgttcgcga aattggtgaa gcagttggct tagcatccag ttcaactgtt catggtcacc
      781 tttcacgtct tgaagaaaaa ggatatataa gaagagatcc aacgaaacca cgtgctatag
      841 aaattgtaag tgatcaaaca aatgataata ttaatatgga agaaacgatt catgtgccag
      901 ttattggtaa agttacagca ggtgttccta ttaccgcagt agaaaatatt gaagagtatt
      961 ttccattacc tgaacactta acatcgacac ataatagcga catattcata ttaaacgtcg
     1021 taggcgacag tatgattgag gctggtatat tagatggaga caaagtaatt gttcgcagtc
     1081 aaaccatagc agaaaatgga gacattattg ttgctatgac tgaggaagat gaagcaactg
     1141 tcaaacgctt ctataaagaa aaaaatcgtt atcgattaca acctgaaaat agtacaatgg
     1201 agccaattta cctcgacaat gttgctgtaa ttgggaaagt aattggtttg taccgcgaaa
     1261 tgtaatattt taaaccgtta tatattatcg taattgttaa accctcattt ttataaattt
     1321 tggacctctt gaaaaagttt cgttttcaag aggttttatt tattctaatc taaattcagt
     1381 tcaaacagaa attgcgaatt gttagataat ctcattcttt aatataaatt ttgaattaca
     1441 gattcctgca aaatgttaca caaattaaaa ctcgcccccg taataattta caggagcgag
     1501 ccattattat ttttttcttt cttttttatt aagtatatct ttgacgcgtt caagtatatc
     1561 cgcatctttt ggtttatctg ttgtcatcat gtttatctac ttccttacag acaaacatta
     1621 ccctttaaac tcataccaaa aacattaacc agtataattt tctgtccaaa aaggttgttt
     1681 ttcatcatta aaatcaacac ca
//
LOCUS       DAHHRR010000226         1686 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.231, whole genome shotgun sequence.
ACCESSION   DAHHRR010000226 DAHHRR010000000
VERSION     DAHHRR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.231"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(153..1634)
                     /gene="cls"
                     /locus_tag="NBI42_002680"
     CDS             complement(153..1634)
                     /gene="cls"
                     /locus_tag="NBI42_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCD1779020.1"
                     /translation="MQFSFSNDLGTLFTIILAIAFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDALLKQQIESFDTGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKIDHFIDGNDLYDQVLQDIKNAKEYIHLEY
                     YTFALDGLGQRILKALEEKLKQGLEVKILYDDVGSKKVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATYSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEMVSVGTANMDFRSFELNFEVNAFVYDEKLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
BASE COUNT          581 a          296 c          215 g          594 t
ORIGIN      
        1 atattagtaa atttattaga tttaaaatag aatactttgt catagatttg aaatgacaaa
       61 tgtcattatt agctttacat aatatattta tcgttattta actttgggta aaataaaaag
      121 agcctctata atcgagactc cttataaata aattataaaa ttggcgaaac taattttgct
      181 aacgattctt tgaatttaac agacagcggt ctattggcat atgattcttt ggttagttgt
      241 tttgattttg taatatcatg ttcataagcc acccttaaat ctttagcaag cttttcatca
      301 tatacaaaag cattgacttc gaaattcaat tcaaaacttc taaaatccat attagcagtt
      361 ccgactgaaa ccatttcatc atctatcaaa cacattttag aatgtataaa tccattttca
      421 tacgtataaa tttttacgcc actagataat aaatctgaag catttgaata tgtcgcccaa
      481 tatactaatg gatgatctgg cttacacgga atcattaagt gtacatctac acctgattta
      541 gctgcaatct ttatagcatt aatataagaa ttgtctggaa tgaaatatgg tgattgtaaa
      601 tatacagatt tctttgcgct cataatcatt tttgtataac catattcaat ttgatgccag
      661 tcactagctg ggccacttgc agctatttga attggtgaat tacccaatgg tccgttcttt
      721 ttagggaaat acttaacatc atattcaaat tgtggacggt gtgcttgtga attccagtct
      781 aaaataaagc gcaattgtaa tgcatcaact gcatctcctt gaattcgtaa atgtgtatct
      841 ctccaatatc ctagtttacc taaaccaagg tattcatctc caatgttaaa tcctccgaca
      901 taacctaatt gaccatcgat tacgatgatt tttctatgat ttctattatt cattctgaaa
      961 ttcaataacg gtaatttaga agcaaaaaat gcttcaactt ctccacctaa cgatttaaaa
     1021 tgatcaaaat tagccatctt aactttcttc gaaccaacgt catcatataa aattttcact
     1081 tctagacctt gtttcaattt ctcttctaac gcttttaaaa ttctttggcc taagccatct
     1141 agagcgaatg tataatactc taaatggatg tattctttag catttttaat atcttgtaaa
     1201 acttgatcat ataaatcatt tccatcaata aaatggtcaa ttttattatt ttcagttaaa
     1261 aagccatctt gatccattaa caacatacgt accaaatcat ggtgcttttg aacttgttta
     1321 ttatctgtac cataattacc tgtatcaaag ctttcaattt gctgctttaa aagcgcatca
     1381 aaatctgtta acacgttccc attattttta tttagtttac gcgcagaaac cgttcttcca
     1441 aaaaatagat aaaggataaa accaattaat ggtaatacaa aaagcacaaa taaccacgcc
     1501 cacgttgaac tggctgtacg tctatttctt tctaaaaaaa taataataaa tgctagaacc
     1561 aaattaatga taaaggcaat tgctaaaata atggtaaaaa gtgttcctaa atcatttgaa
     1621 aatgaaaatt gcatagttta tttctcctat aaagaaaggc acaaaaacct tttgtacctt
     1681 tcgttc
//
LOCUS       DAHHRR010000227         1682 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.232, whole genome shotgun sequence.
ACCESSION   DAHHRR010000227 DAHHRR010000000
VERSION     DAHHRR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.232"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            27..566
                     /locus_tag="NBI42_002681"
     CDS             27..566
                     /locus_tag="NBI42_002681"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCD1779021.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQNDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQSA"
     gene            578..>1682
                     /locus_tag="NBI42_002682"
     CDS             578..>1682
                     /locus_tag="NBI42_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCD1779022.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFNKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDN"
BASE COUNT          617 a          190 c          285 g          590 t
ORIGIN      
        1 tagaattgaa taaagtgggt gataatgtga ctaaaaatga gattagaaaa tacattttac
       61 ataaaatgaa gaattttaat aaagctgaaa agagaaaagc agacacatgg ttaagaaatc
      121 aattttttgc aactgaagaa tacaaagaag caaacgcaat tgcgctagtt ctttctttta
      181 atcatgaagt agatactttt tctataattg aacaagcctt aatggatcat aaacgtattt
      241 ttgtaccgaa aatggattat ttaaaccatc aaatgacttt taaagagata tttaatctca
      301 aagatattga tgtcgataat aaggggattt actatccaac ttcaaaaggt gaaacaacga
      361 ataacctaga tttaattgtt gttcctggtg tcggatttca aaacgatgga tatagaattg
      421 ggtatggtgg tggctattac gacaggtttt tagctaatta tcagacaaag acaataagct
      481 tattatacga ttttcaaata acatcatttg aaccggaatc attcgatcaa ccagtcgata
      541 aattgattat atatcaatca gcatagtgga ggactaaatg aacatagaca aacaattttg
      601 gaaaacaata tattattgga ttaggtactt gaattttgat attgtcagta gggaaaaaga
      661 tgatcaagaa atttggttag cccataaaag aaaaaaacaa gtagtcattt ttaaacaaca
      721 tattaagtct acccaagaaa tacgttttaa caaagcaaaa gtgcttgaac acaaagatga
      781 aatagcaaat tttatttctt tcgaaccaca aagttttgaa ttttattatt ttacagaatc
      841 ggaattttca gaagaacaat taaatgaagt ttcgccaatt agaattaaat tcaatgttat
      901 aagacacaca aaagatttga taaagcatat gccgaatata tttttggcta gacttatttc
      961 agaagataat gataaaaaga catatatgtt ctataaacgc aaagtattaa ccgataactt
     1021 tttagataaa tatatgcaga aattttcacc ggcaacatac acaataatat ttgtaaatgt
     1081 cttaatatgg ttatgtatga ttttatattt aaataatttt tcggatgtaa aattattaga
     1141 tgttggcggg ttagtgcatt ttaatgtcgt acatggtgaa tggtatcgaa ttgttacatc
     1201 gatgttttta cattttagtt ttgaacatat acttatgaat atgctttcat tatttatttt
     1261 tggtaaaata gtcgaagcaa ttattggttc atggaggatg ttaactgtat actttattgc
     1321 agggctgttt ggaaactttg tatcactatc atttaatacg actacaattt cagttggggc
     1381 tagtggtgct atatttggtc tgattggatc aatttttgcg atgatgtatg tttcaaaaac
     1441 atttaacaaa aaaatgttag gacagttatt aattgcatta gtgatattag ttggtgtttc
     1501 tctgtttatg tcaaatataa atattgtggc gcatattgga ggattcattg gtggtttatt
     1561 aataacttta attggctatt actataaggt gaatcgtaat attttttgga ttttactaat
     1621 tggtatgctt gttatattta ttgcacttca aattagaatt tttacaatta aagaagataa
     1681 ta
//
LOCUS       DAHHRR010000228         1680 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.233, whole genome shotgun sequence.
ACCESSION   DAHHRR010000228 DAHHRR010000000
VERSION     DAHHRR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.233"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            303..1070
                     /locus_tag="NBI42_002683"
     CDS             303..1070
                     /locus_tag="NBI42_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1779023.1"
                     /translation="MKFVFDIDGTLCFNGKSIDPSIIDALLQLQNAGHELVFASARPI
                     RDLLPVLPEVFHQHTLIGANGAMISQQSNISVIKPIQACTYHHILRIIQKYELDYIID
                     DDWNYAAKLDAENAIFERLDPHKLASCINVANIDTPIKIILLNIEPPQITSILNELNE
                     YRQELEIIHHSNEFNIDITAQNINKYTALQYIYGKDVEYIAFGNDHNDIVMLQYANNG
                     YIVGPSEDYTHTILKLDHVTHIDSHAQAICSSLNLYV"
     gene            complement(1122..1601)
                     /locus_tag="NBI42_002684"
     CDS             complement(1122..1601)
                     /locus_tag="NBI42_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCD1779024.1"
                     /translation="MGLLDIASIRSIERGFSYYQSKCVINLKSYSETQFEAEVKGSGS
                     NVYHCYIDMEHPRKSKCNCPHADGRRVICKHLIALLFTASPEAANKHIIMLNEVEEDY
                     HLRRNMWIDSLKEMVNDMSEEELRDAYLNMLIEHGEMAELFGLDEEEDLFEDEDEFY"
BASE COUNT          585 a          272 c          224 g          599 t
ORIGIN      
        1 atagattttc taaattttgt cgcgttaatt ttagtttatt tatgtactaa tcctaataat
       61 agaggtattg ataaaatagc atgtgttaaa taaaaagtta atagtaacta caaacatact
      121 ttactaataa tcaaaacgac ttatacaaac tcgaagttat acaagtcccc ttatggaatt
      181 tcacatccta gtttacataa tatatattat aggaagttgc atatctagta caaaaggtgc
      241 cctacattat catttactta tatatgctat aatcatatta ttctattgtt tggagtgatt
      301 tgatgaaatt tgtatttgat attgatggta cactttgttt taatggtaaa tcaattgatc
      361 catcaatcat tgatgcatta ttacaattac aaaatgctgg tcatgaactt gtatttgcat
      421 ctgcacgacc gattcgtgat ctgttaccag ttttaccaga agtctttcat caacatacat
      481 taattggcgc aaatggtgct atgatttcac agcaatcaaa tatttcggtt atcaagccaa
      541 ttcaagcttg tacatatcat catatcttac gaataataca aaaatatgaa ttagattata
      601 ttatcgatga tgattggaat tatgctgcta aattagacgc tgagaacgcg atttttgagc
      661 gcttagatcc acataagctg gctagttgta ttaatgttgc aaatatcgac acaccaatta
      721 aaattatttt attaaatata gaaccgccac aaattacaag cattttaaat gaactaaatg
      781 agtatcgtca agaactagaa atcattcatc attcaaatga gtttaacatt gatattacag
      841 cgcaaaatat taataaatat acagcactac aatacatata tggtaaagat gttgaatata
      901 tagcatttgg taatgatcac aatgatattg tcatgctaca atatgctaat aatggctata
      961 ttgtagggcc atcagaagat tatacacata caatattgaa attggatcat gtgacacata
     1021 tagatagtca tgcccaagct atttgcagtt cattaaattt atatgtataa aaacatcccc
     1081 ctatcaattg ataattatta tcatttaata gggggatgtt tttaatagaa ttcgtcttcg
     1141 tcttcaaata aatcttcttc ttcgtctaat ccaaacaact ctgccatttc tccatgttca
     1201 attagcatat ttaaatatgc atcacgaagt tcttcttcac tcatatcatt aaccatttct
     1261 ttaagagaat caatccacat atttctacgt aagtgatagt cttcttcaac ttcgtttaac
     1321 atgattatat gtttatttgc tgcttctgga ctagctgtaa ataataatgc aattaaatgt
     1381 ttacatatca cgcgtcttcc atcagcatga ggacaattgc atttggattt tctaggatgt
     1441 tccatatcaa tatagcaatg atatacattg ctaccactac cttttacttc agcctcaaat
     1501 tgagtttcag aatatgattt taagttaatg acacatttac tttgataata actaaagcct
     1561 ctttctatag aacgtatact tgcaatatca agtagtccca ttatgacact ccttttaaat
     1621 attcgttatt taagcgaaaa taaaggtaat atacacttaa attaagatta ccgatttatc
//
LOCUS       DAHHRR010000229         1663 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.234, whole genome shotgun sequence.
ACCESSION   DAHHRR010000229 DAHHRR010000000
VERSION     DAHHRR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.234"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            161..1615
                     /locus_tag="NBI42_002685"
     CDS             161..1615
                     /locus_tag="NBI42_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1779025.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHAYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
BASE COUNT          529 a          264 c          274 g          596 t
ORIGIN      
        1 ttataattta accccatcaa aattacaatt aaacatgctc aagatgatta aaaaatcaga
       61 aaattttaat tttttcgctt tacagtacta aatagttatt gaatagtaca cttaaaaagc
      121 ttacaataat gggtatattt aaatagatgg gagactaaat atggaagata ataaaatgaa
      181 ccgtagtctt aactcaagac acatttccat gattgctata ggtggtgcaa ttgggactgg
      241 tctatttgta gctactggta atatcatttc tcaagctggt cctggaggcg ctatactcgc
      301 ttatcttgtt attggtgtca tgctatattt cttaatgtca tcaattggag agttggcaac
      361 attttatcca gtatcaggtt cattcagctc ttattcaaca cgctttattg actcatctct
      421 aggctttacc atgggatggt tgtattgggc attgtggtca ttagttacaa gtgttgatgt
      481 catagtagcg tcaaatgtgc tttatttctg ggacacattt aaatttttcc accccattac
      541 ttggagctta atctttatta caattttact attattaaac attttttctg taaaatcatt
      601 tggagaaact gagttttggt tatcattgat taaagtgtta acaattatcg tattcgttat
      661 ttttggcttt ttaatgattt tcggtatctt aggtggtcat gcatatggat ttgaaaacta
      721 tacaaaaggc caagcaccgt ttgttggtgg tatctctggt ttcttaggcg tattattagt
      781 cgccggattt tcggttggtg gtacagaagt agtagcagta actgctggtg aatcagatga
      841 ccctaaaaag tctatgccta aggcaattaa acaagtattt tggcgtattc ttttattcta
      901 tgtcttatca attgcagtaa ttggtgcaat tattccgtac acagatccat cattattaag
      961 agcaagtagt tcaataagtc aaagcccatt tacaattgta ttcgatagag taggcatagc
     1021 ctttgcagca tcagtaatca acgcggttat tttaacttca ttattatccg ctgcaaattc
     1081 aggtgtttat acaacaggaa gaatgttgta ttccttaagt tcagacaaaa aagcacccca
     1141 atttttaagt aaattaaaca agacaacaaa gttaccttta agagcactat taactactta
     1201 tgcagtcgtt gttattgtta ttatttatgc aaactttaat tcaaatgccg tttttaattt
     1261 acttgaaatt attggttcaa tgattatagt tgtatgggga tcaagcattt ggtcacaaat
     1321 acgattgcga caagctatta aaaaacaagg tcaagatcct aataaggtcc taccatataa
     1381 agcacctttt tatccattag gaccaatcat tgtcatcact acgctattat tcttgctatt
     1441 tggtggttca gttgaatata ttttaaaaga tcaatggtta aatgctttta aaaacttttt
     1501 acctttaatc attctagcgt tgatttactt tattcataaa atcattcaca aaacaaaatt
     1561 tgtaaagcta gaaacaatta atttaaaacc acacgattat gacaatcaaa aataaataaa
     1621 ttctcacacg gcagatgctt tagattatct atagcatttg ccg
//
LOCUS       DAHHRR010000230         1592 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.235, whole genome shotgun sequence.
ACCESSION   DAHHRR010000230 DAHHRR010000000
VERSION     DAHHRR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.235"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..473
                     /locus_tag="NBI42_002686"
     CDS             <1..473
                     /locus_tag="NBI42_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1779026.1"
                     /translation="IGLLSHDGVINQFFHLFNLPSFNLLFTNGAFLVVASYIYIPFMI
                     LPIFNSMKAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMI
                     TRLIAGNKVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            479..1288
                     /locus_tag="NBI42_002687"
     CDS             479..1288
                     /locus_tag="NBI42_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1779027.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            1288..>1592
                     /locus_tag="NBI42_002688"
     CDS             1288..>1592
                     /locus_tag="NBI42_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD1779028.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDH"
BASE COUNT          521 a          213 c          265 g          593 t
ORIGIN      
        1 ttataggttt attaagtcat gatggtgtga ttaatcaatt tttccactta tttaatttac
       61 catcattcaa tttgttattt acaaatggtg cgtttttagt agtggcaagt tacatttata
      121 taccatttat gattttaccg atatttaata gcatgaaagc aattcctaat aatttattgc
      181 aggcctcaag tgatttaggt gctagtcctt tctatacttt tagaaaagta atcatgccgt
      241 taacaaaaga aggtgttatg actgggattc aagtaacctt tattccatca ctttcattat
      301 ttatgattac aagattaatt gcaggtaata aagtcataaa tataggtacg gcaatagagg
      361 aacaattttt aacaattcaa aattatggta tgggatcaac tatagctata ttcttaattg
      421 tatttatggc attcatttta atcattacaa aatcatctaa tgggagaggg tgaatcatat
      481 gaaatggtat ggaaagctgt atatcgggat acttttagcg attttataca tcccaatatt
      541 ctttttaatg ttctattcat tcaattcggc tggtaatatg attcactttg aacattttac
      601 attagagcat tatcaatcat tatttcaaaa tgatcattta atgtcggtca tttttaatac
      661 gatagctgta gcacttttag cagcctcaat ttctacagtt attggtacat ttggtgccat
      721 tgctatttat tatttaagaa ataaaaagtt taaagtaact ttactaacat tgaataatgt
      781 cttgatggtt tcatccgacg ttgtcatagg tgcatcattc ttaattatgt ttacaacgat
      841 tggccatttt actggtcttg gtttaggatt ttggacagtt ctaatatctc atattgcatt
      901 ttgcatacct atagttgtga ttatcgtctt accacaactg tatgaaatga ataataatat
      961 gttaaatgct gcaagagatt taggagcgac tgaaccacaa ttattaagca acattattat
     1021 tcctaatatt ttaccttcta ttataggagg attctttatg gctttaactt attcacttga
     1081 cgatttcaca gtaagtttct tcgttactgg taatggcttt agtgtgttat cagttgaagt
     1141 ttatgctatg gcgagaaaag gaattagtat ggaaattaat gcgatttcaa cattattgtt
     1201 tgctgttatt gtattaggaa tactaggata ttatttgatt caatacgtaa taaataagaa
     1261 aaaactaatc aagcgaggtg taaaataatg aaacgttttt tacaactcat tataggtgca
     1321 ttagttgtgg gtatgctttg tcttacttta agtcattggt ttaaatctaa agaacaagtg
     1381 catacaaatc aaaaaattta cgtatacaat tggggcgaat atattgatcc agagttaatt
     1441 aagaaattcg aaaaagaaac tggcattcaa gtcgtttatg aaactttcga ttcaaatgaa
     1501 gcgatggaag ccaaaattcg caatggcggt acacattatg atgttgcttt tcctagtgaa
     1561 tatacagttc aaaaattgaa aagagatcat tt
//
LOCUS       DAHHRR010000231         1579 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.236, whole genome shotgun sequence.
ACCESSION   DAHHRR010000231 DAHHRR010000000
VERSION     DAHHRR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.236"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            44..1504
                     /gene="ebpS"
                     /locus_tag="NBI42_002689"
     CDS             44..1504
                     /gene="ebpS"
                     /locus_tag="NBI42_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HCD1779029.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDNHETVKDAENNTEHSTVSDKSEA
                     EQSQQPKPYFATGANQSETSKDKHDNVTVKQDKDESKDHHSGKKGAAIGAGTAGVAGA
                     AGAMGVSKAKKHSNDAQNKSNSDKVNNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNNDEHDHNDRDKEPKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKDDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
BASE COUNT          670 a          270 c          289 g          350 t
ORIGIN      
        1 ttttatgtaa aatcattgta atagaataga aaggaagatg gctatgtcta ataattttaa
       61 agatgacttt gaaaaaaatc gtcaatcgat agacacaaat tcacatcaag accatacgga
      121 agatgttgaa aaagaccaat cagaattaga acatcaggat acaatagaga atacggagca
      181 acagtttccg ccaagaaatg cccaaagaag aaaaagacgc cgtgatttag caacgaatca
      241 taataaacaa gttcacaatg aatcacaaac atctgaagac aatgttcaaa atgaggctgg
      301 cacaatagat gatcgtcaag tcgaatcatc acacagtact gaaagtcaag aacctagcca
      361 tcaagacagt acacctcaac atgaagagga atattataat aagaatgctt ttgcaatgga
      421 taaatcacat ccagaaccaa tcgaagacaa tgataatcac gagactgtta aagatgcaga
      481 aaataacact gagcattcaa cagtttctga taagagtgaa gctgaacaat ctcagcaacc
      541 taaaccatat tttgcaacag gtgctaacca atcagaaaca tcaaaagata aacatgataa
      601 tgtaactgtt aagcaagaca aagatgaatc taaagatcat catagtggta aaaaaggcgc
      661 agcaattggt gctggaacag cgggtgttgc aggtgcagct ggtgcaatgg gtgtttctaa
      721 agctaagaaa cattcaaatg acgctcaaaa caaaagtaat tctgacaagg tgaataactc
      781 gactgaggat aaagcgtctc aagataagtc taaagatcat cataatggta aaaaaggtgc
      841 agcaatcggt gctggaacag caggtttggc tggaggcgca gcaagtaaaa gtgcttctgc
      901 cgcttcaaaa ccacatgcct ctaataatgc aagtcaaaac aatgatgaac atgaccataa
      961 tgacagagat aaagaaccta aaaaaggtgg catggccaaa gtattgttac cattaattgc
     1021 agctgtacta attatcggtg cattagcgat atttggaggc atggcattaa acaatcataa
     1081 taatggtaca aaagaaaata aaatcgcgaa tacaaataaa aataatgctg atgaaagtaa
     1141 agacaaagac acatctaaag acgcttctaa agataaatca aaatctacag acagtgataa
     1201 atcaaaagat gatcaagaca aagcgactaa agatgaatct gataatgatc aaaacaacgc
     1261 taatcaagcg aacaaccaag cacaaaataa tcaaaatcaa caacaagcta atcaaaatca
     1321 acaacagcaa caacaacgtc aaggtggtgg ccaaagacat acagtgaatg gtcaagaaaa
     1381 cttataccga atcgcaattc aatactacgg ttcaggttca ccggaaaatg ttgaaaaaat
     1441 tagacgtgcc aatggtttaa gtggtaacaa tattagaaac ggtcaacaaa tcgttattcc
     1501 ataatataac tatataaatt gtaactgaac tgctatatac aaacgtgcat attataaagt
     1561 ttctttaaat attttagaa
//
LOCUS       DAHHRR010000232         1541 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.238, whole genome shotgun sequence.
ACCESSION   DAHHRR010000232 DAHHRR010000000
VERSION     DAHHRR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.238"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            80..946
                     /gene="galU"
                     /locus_tag="NBI42_002690"
     CDS             80..946
                     /gene="galU"
                     /locus_tag="NBI42_002690"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCD1779030.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPSQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKELGL"
     gene            complement(1194..>1541)
                     /locus_tag="NBI42_002691"
     CDS             complement(1194..>1541)
                     /locus_tag="NBI42_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1779031.1"
                     /translation="NNKEFFNLFMNIMHSKDSLKYKLNLTFLELSILYTISTRPSEIL
                     NLKDIFTDSRFMYPQIARAVNKLDNKGILIKERSIDDERLVLIKINEVQCSLINTIIV
                     NTSMLVKRRKLFF"
BASE COUNT          575 a          198 c          285 g          483 t
ORIGIN      
        1 cactatgaag gcgttctaat tagtgttaaa atgacgttga tacatagatt taatacttag
       61 gaaaaggagc acattaactt tgaaaaaaat aaaaaaggca atcattcccg ctgctggttt
      121 agggactaga tttttaccag caactaaagc gatgccaaag gaaatgcttc ctatcttaga
      181 taaacccaca atacaatata tcgttgaaga agctgcaaga gctggaattg aagatattat
      241 tatagtgaca ggtcgccaca aacgcgcgat tgaagatcat tttgatagtc aaaaagaatt
      301 agaaatggtt ttaaaagaaa aaggtaaatc tgaattacta gaaaaagttc agtattcaac
      361 agaacttgcg aatatttttt atgtaaggca gaaagaacaa aaaggtttag ggcatgcgat
      421 tagttctgcg cgtcaattta tcggcaatga accatttgcg gtattattag gtgatgatat
      481 tgttgaatct gaagtgccag cagtgaaaca gttgattgat gtttatgaag aaacggggca
      541 ctcagttata ggtgtccaag aagttccgga agcagatact catcgttatg gaattattga
      601 tccattaact aaaaatggac gtcagtatga agtgaaaaag tttgttgaaa agccatcaca
      661 aggtacggca ccatcaaacc tagctattat gggccgatat gtattaacgc ctgaaatttt
      721 cgattattta aaaacgcaaa aagaaggcgc aggtaacgaa attcagttaa cagatgcgat
      781 tgagcgtatg aataatgata accaagtgta tgcatatgac ttcgaaggcg aacgttatga
      841 cgttggagaa aaattaggct tcgttaaaac taccatagaa tatgcgttga aagatgacag
      901 tatgcgagaa gaattaacac gatttattaa agaattaggt ttataacatt aaaaagcatg
      961 atactcaata aaattggaac acgaaatggt gaactaaatt gaatgaatgt catgcttaat
     1021 ttatgtgttg tgatatatag ctatggagat ttgtcttcaa taaatttagg tataattttt
     1081 aattgctgtt cagagaaatc aaaaatagga aaaacatcat aattaatcac tataaaattt
     1141 ctgatttaat caaatataga gggttataag aaaggtcaat aaaaattctg tattcaaaag
     1201 aagagttttc ttctttttac aagcatagaa gtattaacaa tgatggtatt gattagacta
     1261 cattgtactt cattaatttt aattaatact aatcgttcat catcaataga acgttcttta
     1321 attagtattc ctttattatc taatttatta acagctcttg caatttgcgg atacataaat
     1381 ctactatcag taaatatatc ttttaagttt aaaatttcgc ttggtctagt tgatattgtg
     1441 tataaaatgg aaagttcgag aaaagttaaa tttaatttat attttaaaga atctttcgag
     1501 tgcatgatgt tcataaacag attgaaaaat tctttgttat t
//
LOCUS       DAHHRR010000233         1485 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.239, whole genome shotgun sequence.
ACCESSION   DAHHRR010000233 DAHHRR010000000
VERSION     DAHHRR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.239"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..502)
                     /locus_tag="NBI42_002692"
     CDS             complement(<1..502)
                     /locus_tag="NBI42_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779032.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFL"
     gene            complement(516..1307)
                     /locus_tag="NBI42_002693"
     CDS             complement(516..1307)
                     /locus_tag="NBI42_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCD1779033.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
BASE COUNT          470 a          257 c          200 g          558 t
ORIGIN      
        1 ttaaaaatgc atgttctaaa tacaaaaata ataaatctgt atattgtgtt ttagtacgat
       61 acactttaat ttgagattcc gtatatgata tccgtgtatc taagcgaaga tcaattaatt
      121 tagcagtact tgggcgctca actttaatag aatttaatac gcgcatatct tctaataatt
      181 taaaaagttg ttgataaaat ttagggtgtt taaaggtttt atcttgtact acttcattta
      241 caatttgtac atccatcata tgataaccgt aagcagctaa cataacatct gtttttaaac
      301 cagccatttc aatatggctt ggtcgatgtg accaaaacca actcgtaatc aaaacatttt
      361 gaacaggatt atagtaaggg aatttttgga ttttaacttg tgtttgttca ttttttaata
      421 agagtcgagc taagtcttgc aacatcatta cttgcttggc atctaactgt tcgtcgttaa
      481 attttatgaa acgatcactc atgttttatc cctctttaaa agtttagttc tacagcatta
      541 tatattgctt gttgttcacg ttcatcttcc aatttatcaa taattgtacg tttaattgca
      601 cgttcaactg gcattacagt gattaaatca cacaagtcta ataatgcacg gatactagcg
      661 gcttcttcag aaatttgtcc ctgcttagac atagtacgta aatcttcgtt aaacttaata
      721 atttgttcga tttgtttatc atcttgtaat aaactttgct ctttaatcac attttttaaa
      781 atgtccccat caatatagtc aacgtgaata acaacaaagc gattttttag tgcttcattc
      841 attggcaatg taccaacata accttcattt atcgctgcta taacgttaaa tcctggtaca
      901 gctttgatta cttcaccagt gtatggattc gtaatttgac gacgataatc taagacccca
      961 tttaatacag gcaatgtttc aggtttagcc atatttattt catcaatata taaaatatgc
     1021 ccctctttca tagctttaat aactggacca tctacaaaga caatttcttg ttgtccttcc
     1081 gcatttgttt taattgtttt aaagcctaat aagctttctg tatctaaatc aacagaacaa
     1141 ttgacttgat gcatgggtgt atcaacaact tcacttaatg tttctgccaa cttcgttttc
     1201 cctgaacctg ttggaccttt aagtaaaata tttttattta aatcaaataa tgccttcgca
     1261 tcattgaaaa ctgttgaatc tgaattctta taatgtttta gtgccatttt aaaacgttct
     1321 cctttaaata aattaaaaaa ctaagacatg gaataagttc tccaaatgcc ttagtttact
     1381 attctattct gatataaaac gcaatgctta gtatgcgaat tgtttatatt ttaccaaatt
     1441 tactaattga aaatctcata ttaggcttca tttcaataat tatgc
//
LOCUS       DAHHRR010000234         1481 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.240, whole genome shotgun sequence.
ACCESSION   DAHHRR010000234 DAHHRR010000000
VERSION     DAHHRR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.240"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            24..353
                     /locus_tag="NBI42_002694"
     CDS             24..353
                     /locus_tag="NBI42_002694"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779034.1"
                     /translation="MDIDFFVDASAFNNKILLSRVEMTFNKYNIGQTYEILAKTAFNN
                     QVDSFLRTFIASGEDKLKEDIYNIIKHYNDENRWEYVFYNEIISQNTWPSIIKAFNKY
                     IEFNKNV"
BASE COUNT          523 a          210 c          194 g          554 t
ORIGIN      
        1 ttgataaaat agagaatttt attatggata tagatttctt tgttgacgct tctgcattca
       61 ataataaaat tcttttaagt agagtagaaa tgacgtttaa caagtataat attggtcaaa
      121 cttatgagat tttggcaaaa acagcattta ataatcaagt tgactccttt ttgcgtactt
      181 ttatagcatc tggagaagat aaattgaaag aagatatcta taacattatc aaacattata
      241 atgatgaaaa cagatgggaa tatgtatttt ataatgaaat aatatcccaa aatacttggc
      301 cttcaataat aaaagccttc aataaatata tagaatttaa taaaaacgta taataagtta
      361 ctttccaaga ggtattatca cagtataaaa agtaacccat aaatctatgt atttgtgcaa
      421 attatttagt aattgaaagt tctagccgat ctaaaaccac gactttgaga aatgctgtct
      481 cggaatgagg ttcaatctga tgatatcatg cagccaaaaa aatatagtta ttaaaggaat
      541 catatgggaa acaggttaat taaatctgat aattaagaaa aagctcttaa ccaattttta
      601 aaagagtcat tagaatgctc aaatattaat tctgttacgt tagcaattca taaaaaacta
      661 gtaaatcaca ttcgtaaata aggttagaat gtatggaaaa taatatgatt tgaaatattt
      721 atgacagcac gttatacgtt tgaaccatct ttagtgattt tattatcttt tctttttata
      781 gaattataat aggtacttct tggtattttt aggactttac acattgctgg tactgaatgc
      841 tgatgtacat ttttttgaat gacttctatt ttcgtcgcat aatcagcgct gcttgcttta
      901 aaatatcgtt ctccatcttt atatattgaa cctctttacg taatttaatc agctctattt
      961 cttcatctga taagttatct tggtgattga atgaacctgt gtattaatgt tgctttatcc
     1021 attttcctaa cacctaggaa tgaaatctaa ccatttgtaa cttaaactct gaactaaacg
     1081 attttctttc tcttgtcata ataaaatcgc ccactttctt aaattaacaa tatctactct
     1141 catagaattt gtctaattaa gtgtagacat ttcaaaatat caaatttata tgtatagtac
     1201 ttcttgcttt ttatgattaa cttagcaaaa aatacaaatc aacaataaat aaaatttaat
     1261 tacttatata tatttattag cattagagtc tgtttacagc atcccttttg atttgttcat
     1321 cagatttttc tttccattca tgaggtgaaa agttaaatgt aatactatgg tcatcaaaaa
     1381 actcataaag agcaggtctt aacctttttg ctttttcatt tcttctttct gatctttttc
     1441 gaccttctga taatcgtgta acacctgtac aataaatttt a
//
LOCUS       DAHHRR010000235         1481 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.241, whole genome shotgun sequence.
ACCESSION   DAHHRR010000235 DAHHRR010000000
VERSION     DAHHRR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.241"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..535)
                     /locus_tag="NBI42_002695"
     CDS             complement(<1..535)
                     /locus_tag="NBI42_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCD1779035.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKVHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTHVTNIYGK"
     gene            752..844
                     /locus_tag="NBI42_002696"
     CDS             752..844
                     /locus_tag="NBI42_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779036.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            complement(926..1426)
                     /gene="ftnA"
                     /locus_tag="NBI42_002697"
     CDS             complement(926..1426)
                     /gene="ftnA"
                     /locus_tag="NBI42_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HCD1779037.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
BASE COUNT          488 a          222 c          231 g          540 t
ORIGIN      
        1 ttttaccata tatatttgtt acatgtgtta aattctcata gtttttcaat aatctaaagg
       61 taatcattgc acacgctttt gcatcattta aggcatcgtg atgaccatga aaatctaatt
      121 ggtaaaattc catcatatgt ttcaaaccat atcgataaga atctactgtt cttttagcaa
      181 gttgataact acaaaagtaa gttaaatttg gagttggtaa accaatattt tgaatgcttt
      241 gatgtaagac gttcatatca aatgccgcgt tatgtgcgac aacaggtaaa tctgcaataa
      301 attgcatcat atatggaaat acgtagtcaa acgtaggtgc attttcaaca tcttctggtt
      361 gtatgccatg aactttaata ttttgttgtg aaaaatagtc ttgcggattc acaagagtat
      421 gaaatgtttc tgttatttga ctatcaatga ctttaaccat tccgacagaa caaatacttg
      481 tacgtttacc atttgctgtt tcaaaatcaa gtgcaacaaa cgcatcttgt atcatgtagt
      541 caatcctttc aattaattaa atgtatatat taatagactt tttagtcatt tgcttacaaa
      601 cgatacataa tattatacgt gaaggtataa cgtgaaatct atcatttgga tgtaactctg
      661 attaaaaagt aaatgatgat atgctttaaa atcggtcaaa ttaattcaac attaaattta
      721 gagttattaa cattagtagt ttaaaaaata aatgactaaa aatacaataa ttagccttga
      781 aaatgaaaaa acgcagatca atgattcaga aaatgaatct agtgatctcc gtaaagcaaa
      841 ataatataga cattgggtta ttgtatgtga cccaaatgtc tatcatgtcg tatgatattt
      901 atctgtctat tgtagtgatg tttaattatt cttcgtcgaa tgtacgagcc ccaagttctt
      961 tttcgtaaag atataatgca ttgctgtcat cgccgatacg agttaaataa ttgatgtgag
     1021 tttcaaacat tgattcttct tcgacttgtt catctaagaa ccagtttaag aatgagatag
     1081 ttgcataatc tttatcttga cgagcgattt cagataagtt atagaaacgt cttgtcactt
     1141 cttgttcttg agataagctg tctttgaaag tttctagtat gcttgaaaag tcaatttttg
     1201 gtgctgaaac tgctctgaat tctgcatgtg cacctctgtc gttaatatag ttataaatct
     1261 tttgtccatg gaaacgttct tctttagctt gttgaatgaa gaagtttgca aatccttcgt
     1321 acgattcttt atcacagtat gctgccattg ccatatatgc atgtgctgca aagtactcat
     1381 ggttcatttg atcatttaat gcttctaata aatttttact taacattttg atacacctct
     1441 tatttgtaaa ttaaaattac tatgaattaa acttagtata a
//
LOCUS       DAHHRR010000236         1477 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.242, whole genome shotgun sequence.
ACCESSION   DAHHRR010000236 DAHHRR010000000
VERSION     DAHHRR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.242"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            244..1203
                     /gene="hyl"
                     /locus_tag="NBI42_002698"
     CDS             244..1203
                     /gene="hyl"
                     /locus_tag="NBI42_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HCD1779038.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN"
BASE COUNT          566 a          215 c          224 g          472 t
ORIGIN      
        1 agaattcatt caactttgac taaccctcga aattgaaatg cttcctttca aattttaaat
       61 aaaagttaaa aacatattct taaaataatc actcatcatc actcagtaat ttatcagttg
      121 ctacattaaa tatactaata taaacgatta taaatatttg atatgtctca actgcattat
      181 tctaaattga catattgatt attgtttcct caaatcgttt taaaaaatag aaggatgatg
      241 aaaatgaaaa cacgtatagt cagctcagta acaacaacac tattgctagg ttccatatta
      301 atgaatcctg tcgctaatgc cgcagattct gatattaata ttaaaaccgg tactacagat
      361 attggaagca atactacagt aaaaacaggt gatttagtca cttatgataa agaaaatggc
      421 atgcacaaaa aagtatttta tagttttatc gatgataaaa atcacaataa aaaactgcta
      481 gttattagaa cgaaaggtac cattgctggt caatatagag tttatagcga agaaggtgct
      541 aacaaaagtg gtttagcctg gccttcagcc tttaaggtac agttgcaact acctgataat
      601 gaagtagctc aaatatctga ttactatcca agaaattcga ttgatacaaa agagtatatg
      661 agtactttaa cttatggatt caacggtaat gttactggtg atgatacagg aaaaattggc
      721 ggccttattg gtgcaaatgt ttcgattggt catacactga aatatgttca acctgatttc
      781 aaaacaattt tagagagccc aactgataaa aaagtaggct ggaaagtgat atttaacaat
      841 atggtaaatc aaaattgggg accatatgat agagattctt ggaacccagt atatggcaat
      901 caacttttca tgaaaactag aaatggttct atgaaagcag cagataactt ccttgatcct
      961 aacaaagcaa gttctctatt atcttcaggg ttttcaccag acttcgctac agttattact
     1021 atggacagaa aagcatccaa acaacaaaca aatatagatg taatatatga acgagttcgt
     1081 gatgactatc aattacattg gacttcaaca aactggaaag gtactaatac taaagataaa
     1141 tggacagatc gttcttcaga aagatataaa atcgattggg aaaaagaaga aatgacaaat
     1201 taatgtaaat tatttgttca tgtacaaata aatataattt ataactttag ccgataactt
     1261 cagaaatgat gtttttcggc taatttttat actgaattta gtttaattaa cttatgatat
     1321 ttttaattta aactatgtac ttgatttgct ttcctgactt aaaaattaga ctaaacatga
     1381 caatatatga gttaattttt aaaataaaat atttgaattc catgttgtca aaccctttat
     1441 ataaaagttt tgaaattgtt caaaactttt tgaacac
//
LOCUS       DAHHRR010000237         1435 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.243, whole genome shotgun sequence.
ACCESSION   DAHHRR010000237 DAHHRR010000000
VERSION     DAHHRR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.243"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            7..180
                     /locus_tag="NBI42_002699"
     CDS             7..180
                     /locus_tag="NBI42_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779039.1"
                     /translation="MNKKATKLLEDYKKVQQRKTEETDRGCLKSVIMLIVLFLFIIFG
                     IVACSTHVNFFFQ"
     gene            complement(415..765)
                     /locus_tag="NBI42_002700"
     CDS             complement(415..765)
                     /locus_tag="NBI42_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCD1779040.1"
                     /translation="MKFKKYIVAGTLAVLLSTTAVSTLDGNKADASSHDNYLTQSKYH
                     DKKIAEELKSLLRQSNVYDLAAGSLNRYYKRTIMMNEYRAKAALKKNDFKSMAEAKVG
                     LENIYKEIDEIINR"
     gene            complement(917..1414)
                     /locus_tag="NBI42_002701"
     CDS             complement(917..1414)
                     /locus_tag="NBI42_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HCD1779041.1"
                     /translation="MKNKLIAKSLLAIAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNTTPKYIKFRHDYNIVEYNDGTFGYGPRPHFNKPAEKSV
                     ATLKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYNVKKMILQERIDQVLK
                     QGLVR"
BASE COUNT          438 a          229 c          184 g          584 t
ORIGIN      
        1 aaacatataa ataaaaaagc gacaaaacta cttgaagatt ataaaaaggt tcaacaaaga
       61 aaaacggaag aaactgatag aggttgtttg aaaagtgtca tcatgcttat tgtcttattt
      121 ctattcatta tatttggcat cgtggcatgt tcgacacacg ttaacttttt cttccaataa
      181 tatacaaaat cagaacgttt catactacgt ttaaatttat ctatgggagc actataaata
      241 ttttttagat tgtacaaagc aattttgtgc ataatatttc taactattat atttatattt
      301 gtctccccta ttttttgtat acattgctca tacataaata ataaatcgca gaattttcat
      361 ctgcataaaa aaacaggcga agacaatggt attattgtct cagcctgttt tttattatct
      421 atttataatt tcatcaattt ctttgtaaat gttttctaat ccaactttag cttcagccat
      481 tgatttgaaa tcatttttct ttagtgctgc ttttgctcta tattcattca tcataatcgt
      541 acgtttgtaa tatcgattta aactacctgc agccaagtca tatacgttcg attgtctaag
      601 taatgatttc aattcttcag caattttttt atcatgatat ttactttgag ttaaataatt
      661 gtcatgacta cttgcatctg ctttgttccc atctaatgtt gatactgctg ttgttgataa
      721 tagtactgct aatgtacctg ctactatata ttttttaaat ttcattgtaa attctccttt
      781 aaaaattgat acttaatgat tatatagtca attttaggaa agttacatta acctactatt
      841 aaataccata gtcgttttat taataaaaaa taatataata ttaaattatt ttcaacttat
      901 aataatgaca gaagtattat ctcactaatc cttgctttaa tacttgatca attcgctctt
      961 gcaatatcat tttcttaaca ttgtattcaa atgatactaa gttgactgcc ttttgtgctt
     1021 ttctatgtgc gctcacagta tgtgtttttt caaattctct cacaagattt tgagcttgta
     1081 ttaatttctg ttcttttttt aatgtcgcaa cagatttctc agctggttta ttaaaatgcg
     1141 gacgtggacc atatccaaat gtaccgtcat tatattccac gatgttataa tcatgtctaa
     1201 acttaatata tttaggtgtt gtattaaatt ttggtctaga ttgatattta aaagtaccat
     1261 cattgtactc tacgatattg tgattaatac tcactggttt cttttctctt ggaccgtatc
     1321 cttcgctcgc atctgctgtt gacgcaattg tagttgtagt gataccaatt gccgctatcg
     1381 ctagtaatga ttttgctatc aatttatttt tcatgttaat tatcctccaa attgt
//
LOCUS       DAHHRR010000238         1400 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.244, whole genome shotgun sequence.
ACCESSION   DAHHRR010000238 DAHHRR010000000
VERSION     DAHHRR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.244"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            107..952
                     /locus_tag="NBI42_002702"
                     /pseudo
     CDS             107..952
                     /locus_tag="NBI42_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            1048..>1400
                     /locus_tag="NBI42_002703"
     CDS             1048..>1400
                     /locus_tag="NBI42_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779042.1"
                     /translation="MTEKPNIENLVNFIKLKESISNYIFVSLGHPLVKAQVKILKNIN
                     YLENDLNKLCQKFKNKSGKYPTWVKLDIVTSQKTIPFHILQDEMINTRRNYIDFGISL
                     DKYWNVTFLPEEINA"
BASE COUNT          497 a          259 c          293 g          351 t
ORIGIN      
        1 aacaccagga acagaagtag atattccagt aaaagtaaca tatccagatg gttcaacaga
       61 agaaacacct gtaaaagtta taatcatttc agatgactca gacgctgatg cggacagcga
      121 ttcagattca gatagcgact ctgatgcgga cagcgactca gactcagact cagactcaga
      181 ttcagatagc gactctgatg cggacagtga ctctgatgcg gacagcgatt cagactcaga
      241 ttccgacagc gactcagact cagattcaga tagcgactct gatgcggaca gtgactctga
      301 tgcagacagc gattcagact cagattcaga cagtgactct gatgcggaca gtgactctga
      361 tgcagacagc gactcagatt cagattcaga cagtgactct gatgcggaca gcgactcaga
      421 ctcagacagc gactcagatt cagattcaga cagtgactct gatgcagaca gcgattcaga
      481 ttcagatagc gactctgatg cggacagtga ctctgatgcg gacagcgatt cagactcaga
      541 cagcgactca gattcagata gcgactctga tgcggacagt gactctgatg cagacagcga
      601 ttcagattca gatagcgact ctgatgcgga cagcgactca gattcagatt cagacagtga
      661 ctctgatgca gacagcgact cagacagtga ctctgatgcg gacagcgact cagattcaga
      721 ttcagacagt gactctgatg cagacagcga ttcagactca gattcaggaa aacatacatc
      781 tgttaaacca atgagtgcta ctaaagatca tcacaataaa gctaaagcat taccagaaac
      841 aggtagtgca aatgatgaat caaataatgg aacattattc ggaggattat tcgcagcatt
      901 aggttcatta ttattattcg gtcgtcgcaa aaaacaaaat aataaacatt aagcaggaaa
      961 aagcttaaaa gccaggtcat agagacctgg cttttaaaat cattaaaaca tttaagctat
     1021 gaaattatat taagtagggg gattattatg actgaaaaac ctaatattga gaacttagta
     1081 aactttatta aattaaagga aagtataagt aactacattt ttgttagttt aggtcatccg
     1141 ttagttaaag ctcaagtaaa gattttgaaa aatattaatt atttagaaaa tgatttgaat
     1201 aagctatgtc aaaagtttaa aaataaatca ggaaagtacc caacttgggt caaattagat
     1261 attgtaacta gtcaaaaaac aattcctttt catattttgc aagatgaaat gattaacact
     1321 aggagaaatt atatagattt tggcatttcg ttagataaat actggaatgt aacatttcta
     1381 cctgaggaaa ttaatgctaa
//
LOCUS       DAHHRR010000239         1367 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.245, whole genome shotgun sequence.
ACCESSION   DAHHRR010000239 DAHHRR010000000
VERSION     DAHHRR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.245"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            78..1286
                     /gene="gltS"
                     /locus_tag="NBI42_002704"
     CDS             78..1286
                     /gene="gltS"
                     /locus_tag="NBI42_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCD1779043.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
BASE COUNT          416 a          198 c          248 g          505 t
ORIGIN      
        1 ttaaaagtgc ccttttataa cgcttacata taaaagctta tttagggaga gggatattca
       61 acaaggggga tttgaaaatg atagaactta atgcaattac aacattatgt ttagcctgta
      121 tactttactt actgggtaaa gctatcgtta atcacgttaa ttttttaaaa cgtatttgta
      181 taccagcacc agtgattggc ggcttaatct ttgctatttt agttgcggct ttggattcat
      241 ttggcatggt taagattaaa ttagatgctt cattcattca agatttcttc atgttagcat
      301 tctttacgac aatcggtctt ggtgcatcat tgaaattatt taaattaggt ggcaaagtct
      361 tgttattata ctttatgttt tgtgctatca tttcagtcat tcaaaacata gttggtgtat
      421 cactagcaaa agtattaaat attaaaccgt tactaggatt aacagctggt tctatgtcta
      481 tggaaggtgg tcacggtaat gctgccgctt atggtaagac aatccaagat ttaggcattg
      541 actcagcatt gacagcagca cttgcggctg caactttagg tcttgtattt ggagggctta
      601 tcggtggacc agttgtaaaa tttttaatca aacgttataa cttaaagcct caacatagtg
      661 acgatacatt taaagattat agccaagtag catataatga acatttacat agtaaattta
      721 atgccactga agtattcttc attcaattta caatcgttgt attctgtatg gcagttggaa
      781 gttatttcag tcatttgttt acagctcaaa cagggattaa tgttccaatt tacgttggct
      841 cattatttgt agctgttatt gtccgaaata tctctgaaag ttttaatttt aatattgtag
      901 atttaaaaat tactaatcaa attggcgatg tcgcattagg tattttctta tctcttgcgc
      961 taatgagcat tcaattaatc gaaatttata aacttgctat acctcttatt attatcgttt
     1021 tagttcaagt tgtcgttatg attttatttg ctgttttaat tttatttaga ggtttaggaa
     1081 aagattatga tgctgcagta atggtaggtg gttttatcgg gcatgggctt ggtgcaacgc
     1141 caaatgccat ggcaaattta gatgttatta ctaaaaaata tggaaactca cctaaagcat
     1201 atttagttgt acctattgtt ggtgcattct taatcgattt aattggtgtt atagtcatta
     1261 tgggattcat tcaatggttt agttaaacac caaactcatg agtctgtgac ataaatccct
     1321 aaaaaaaaca gcagtaagat aattttcaat tagaaaatat cttactg
//
LOCUS       DAHHRR010000240         1351 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.246, whole genome shotgun sequence.
ACCESSION   DAHHRR010000240 DAHHRR010000000
VERSION     DAHHRR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.246"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            89..709
                     /locus_tag="NBI42_002705"
     CDS             89..709
                     /locus_tag="NBI42_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779044.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL"
     gene            1182..>1351
                     /locus_tag="NBI42_002706"
     CDS             1182..>1351
                     /locus_tag="NBI42_002706"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779045.1"
                     /translation="MIKKISLQNIATYRNYVEIKPKKINFIYGSHESTSVTSNKIVII
                     DDPISSLDSNVL"
BASE COUNT          494 a          152 c          234 g          471 t
ORIGIN      
        1 ttaggaggtt ttttgtattt atgctttcaa atgtgtgata tactttgttt gttgaaatat
       61 agagtatcta tagatagggt gattgagtat gaaattcact gaagtggaag ttatcgaaca
      121 tcttgtaaag gcatataaag aagcaggaaa gcctacttat cctcatgaaa atttatatcg
      181 aggacgtaat catagtattt caggtattgg agaagacttg ctgggtgctt atttgattag
      241 tagattggaa ggtgtccaaa tatttattga tcagccttta tctatgattg ataaatcttt
      301 aagtacaaga tatccggatt tattaatttg tgaagataat gaaattaaaa atatactaga
      361 agttaaaatg gacttaggat atcaaagaaa agattttata gattattgcc gaaagaaaga
      421 agaatggatt tcaaatatcg taggaaaaca gtgtgtattg tctagaaaga gagaagacaa
      481 aattcctatg aatatagctg atgatattaa atttcatgtt gtgatttaca gtgaaaacaa
      541 tggaccgaag cggtttgatg aagaaatcat gcctatcgtt aatgaaacat gtccacatat
      601 tgaagtatat gtcctaacaa gcggtcaaca ccctaattta gtaaatgtta atcttgaagg
      661 tattaatatt aataaagatg aatttgaaat attagtaaat gcgttataaa aaaatagagc
      721 atcctccacg ttatggaggt gctctgtttt ttattgaaaa gtatcaagtt aattaattta
      781 atatgcttaa taagttctac cttgaccttt ttctctagct tctgttcgat ctcttatgta
      841 ctcagtacat actggatttt ctgttaattc atcaactgta ttagtttaat cagaagacgt
      901 gtctactttg taagcttcta agaacttatt atatgatata gcgtttgagt tttgttgttc
      961 ttctataaat ttctgtagtt atttttcaaa aaccgcatca ttaactgata agcagaggca
     1021 tatcataaat aggggggaga ttaaatacaa aaataatatt aaatacatta gtaacatctc
     1081 agaaataatt ttgattatca gaaataaatc taaagcccac agttagcagt tgataaatat
     1141 agtgtcttta ctaattaaaa taagggattg aggtattatg aatgattaaa aaaatttcgc
     1201 ttcaaaacat tgcgacttat agaaattatg tagaaataaa acctaaaaaa ataaatttta
     1261 tttatggtag tcatgagagt acaagtgtaa cttcaaataa aatcgttatt atagatgatc
     1321 caatttctag tttagatagt aatgtattat t
//
LOCUS       DAHHRR010000241         1341 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.247, whole genome shotgun sequence.
ACCESSION   DAHHRR010000241 DAHHRR010000000
VERSION     DAHHRR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.247"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..363
                     /locus_tag="NBI42_002707"
     CDS             <1..363
                     /locus_tag="NBI42_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1779046.1"
                     /translation="IKYHYIKTQEKRIDLYFKHNLNNYKNMKVTDFHKNPMGGYFVEG
                     YINDNKDYEFQASIDSGGDDQFHKDIGYDGDKIGKLFKEKDPKYKLSVDEIIEKEHLD
                     KSVYEAEPPLFFFSGRLE"
     gene            363..805
                     /locus_tag="NBI42_002708"
                     /pseudo
     CDS             363..805
                     /locus_tag="NBI42_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
     gene            805..1248
                     /locus_tag="NBI42_002709"
     CDS             805..1248
                     /locus_tag="NBI42_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1779047.1"
                     /translation="MKKKYILIIVSVILIGMIVIAYAHNKQKKDHYIETQEKRINLFF
                     KYNLNQYQSLKVTRFKKLPMDSYFIGGYINNNKDYEFQAFINVGGRKQFQRDIGYDEE
                     KLGKLLKEQEVKNWKTVDQIIKKEHLDKSEYEAEPPLFFFSGSLD"
BASE COUNT          570 a          142 c          225 g          404 t
ORIGIN      
        1 ataaagtatc attacattaa gacacaagaa aagcggatag atttgtattt taagcataat
       61 cttaacaatt ataaaaatat gaaggtgaca gattttcata aaaatccaat gggaggatat
      121 tttgttgaag gttatattaa tgataataaa gattatgaat tccaagccag tattgattcg
      181 ggaggcgatg atcagtttca taaagatatt ggatatgatg gagataaaat aggtaaatta
      241 ttcaaagaaa aagaccctaa atataaactc tctgtcgatg aaataattga aaaagaacat
      301 ttggataaaa gtgtttatga agcggaacca ccgctattct tcttttcggg gagattagag
      361 tgatgaaaaa gaaatatatt ttaataatag ttagtgtaat tttaataggt atgatagtta
      421 ttgcatatgc gcataataaa caaaaaaaga ccattacatt gagacgcaag aaaaaagaat
      481 agatttatat tttaagtata accttaataa ttatcattca atgaaagtta ctagttttaa
      541 aaagacgcct atgggtggct atattgtaga tggatatatt aatcacgaca aaaattatga
      601 ttttaaagtt ctcatctcag caactgacaa tcatcaattt gaagatagca taggatacga
      661 tgataagaca tttggtaaat tattcaaaga aaaagaccat aaaaatgaat taaaacctaa
      721 agatattata aaaaaagaac acttggataa aagtgattat gaagccgatc caccgttatt
      781 cttcttttcg gggagattag agtgatgaaa aagaaatata ttttaataat agttagtgta
      841 attttaatag gtatgatagt tattgcatat gcacataata aacaaaaaaa agaccattac
      901 attgaaacac aagaaaagag aataaattta ttttttaagt ataacctgaa tcagtatcag
      961 tcactgaaag taactagatt taaaaagttg ccaatggaca gttattttat aggtggttat
     1021 ataaataaca ataaagacta tgaatttcaa gcttttatta atgtaggtgg tagaaagcaa
     1081 tttcaaagag atataggata tgacgaagaa aaacttggaa aactattaaa agagcaagaa
     1141 gtaaaaaatt ggaaaaccgt cgatcaaata attaaaaaag aacacttaga taaaagtgaa
     1201 tatgaagcgg aaccgccgtt gttcttcttt tcaggatcgt tggattagat tcggttgtat
     1261 ggtgtaataa aaattattat tatcctcaca atcattaaag tacaagagta ttttaaaaaa
     1321 tcattacata acatacaaaa a
//
LOCUS       DAHHRR010000242         1325 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.248, whole genome shotgun sequence.
ACCESSION   DAHHRR010000242 DAHHRR010000000
VERSION     DAHHRR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.248"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(85..1251)
                     /gene="norA"
                     /locus_tag="NBI42_002710"
     CDS             complement(85..1251)
                     /gene="norA"
                     /locus_tag="NBI42_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HCD1779048.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLVLLVIADGYWTIMVISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
BASE COUNT          487 a          270 c          174 g          394 t
ORIGIN      
        1 cttaataaaa ttcataagaa aaacgatgct aatcattcat aatattacgc tttactttat
       61 atcaatatac accaaaaact tatgctacat attttgttct ttcaattttg ctctatgttg
      121 cttttcaatt aaaacaataa caacacctgc taatgaaaca cctatagcca tataaattgg
      181 tgcttcaatg tgtacatcaa ataacgcacc tgcgattaaa ggacctatga aattacccat
      241 actagtgaat gtcgagttca atccgcctgc aaagccttgc ctttctccag caatattaga
      301 aaaataattt gttatggctg gtcgtatcat atcgaacccg ataaagacaa caaagctaat
      361 taccataatt gtccagtaac catcagcaat aactaatagc actaagacaa caactgaata
      421 taataatgac caagctataa atgttaactc tgagaaatac ttcataaatt tatcgaagaa
      481 atagatttgg aaaagtgccc caaatatacc gccacccgta atagcaatcg aaatatcttt
      541 aggtgaataa tttaccttgt cagctgtgta tagtgaatac aatgtttcaa atgcagataa
      601 accaaacgat aatacaagtg ttaaaataac tggtgtaata aacactttcc agttaatttt
      661 cgttagcaat tgtggctcta acttttggaa accacttgtc gtagactttt tcggatcgtg
      721 aatcaataca attgacatta taaatgctag aatacctaat gctcctgcaa agtaaaatgg
      781 catacgatgt gaaacttctg ccataaatcc accaatacct ggtcctaaaa tgaatccaga
      841 attgataatc gctgacatgt agccaaagtt ttttgctttt tgatggcttg gtgaaatgtc
      901 agctattaaa cctgtcacac caggcattac cataccagca ctcataccac caatcactct
      961 cgataacatc aataccgaaa aattgtggcc aactgcaaac ataaattctg acactgaaaa
     1021 caaaattaat cctatacata taattaattt cttccctaat ttgtcagcta gcgtaccacc
     1081 aaacggcgat ataatcattt gagataacgc aaaagcagca actagtaatc ctaaatcact
     1141 accagttaat cccaaatctt ttaaataaac aggcaagact ggtattacta aaccgatacc
     1201 taaaaaaatc aagaaaatat taaaatataa gacaaaaatc tgtttattca tatgctcacc
     1261 tcttttttct ccatgtcatg cttaaagctg ttattcttct tttccacgac agattgcaat
     1321 tcatg
//
LOCUS       DAHHRR010000243         1323 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.249, whole genome shotgun sequence.
ACCESSION   DAHHRR010000243 DAHHRR010000000
VERSION     DAHHRR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.249"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            670..749
                     /locus_tag="NBI42_002711"
                     /pseudo
     CDS             670..749
                     /locus_tag="NBI42_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            759..1031
                     /locus_tag="NBI42_002712"
     CDS             759..1031
                     /locus_tag="NBI42_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCD1779049.1"
                     /translation="MFDSLLYLTLALGLSTAAYASTEYAEGGTWSHRVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASVTKHNWWVNRAYYNIY"
     gene            1075..>1323
                     /locus_tag="NBI42_002713"
     CDS             1075..>1323
                     /locus_tag="NBI42_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCD1779050.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRINQFAKNENVAIYKSTSNYTNKNVDKD"
BASE COUNT          503 a          174 c          200 g          446 t
ORIGIN      
        1 agaaaagctt ttttgcttaa gaatagcaat acgaagattc aagtcatgct tcattatagt
       61 gtatacatta gtatagtagt cgcaataatt aatttaaaca ataaaaaata accatcattt
      121 ccaaactttg acaacgaatg atggttaaag aaaatctcat ttagtcaccg tctttttaac
      181 gggttcaata ggcgacatgt tcctgcatgt cgtattttta catttttata gtaacatatc
      241 tattatttaa ttgtctattt ttattagatg tgtaacaaaa ttttgaagta tttttcgtat
      301 attgcttgct aaacatgtca gatttgaaag gaaaatcata ctgtggtaga aatcacttat
      361 gattctatgg cagtaattat acataagtga tttctattga atatgtaatg aagttcatat
      421 attaatataa ctttcattgg tatagtattc gcaatgatta atttaaacaa tataaaacaa
      481 ccatcatttc caaactttga caaagaataa tggttaaagc aaatctcatt tagtcaccgt
      541 ctttttaacg ggttcaatag gcgacatgtt cctgcatatc gtctatttat gcactgcaca
      601 atttaataat aatacaatta tactttgatg tccatttaac atgaagtagt tgaaatttgt
      661 agcaaataaa ttcatcttta aatattcaga aaattctgta taattcgaat aaagagatat
      721 ggattaaata tgaagaggtg tttttataga agaataaaat ttttgactct ttattatatt
      781 taactttagc acttggatta tcaacagcag cttatgcatc tacagaatac gcagaaggag
      841 gcacttggag tcaccgtgtc ggcagtaagt atgtttggtc ttattattat catggtcata
      901 aaggacatgg tgcaacagct attggaaaat acagatcatt tagtggttat acaagagctg
      961 gtgtaaaagc aaaagcatca gttactaaac ataattggtg ggtcaataga gcgtattata
     1021 acatttatta aatactaaaa aatcatagaa aataaacaac gcaagaggta caacatgaaa
     1081 tggttcaaat taatacttga tgttacaatc ttcattctga ttgctatatt gttatttgtt
     1141 tatacatata aagaaaacga agaaatattg cctgatacta aataccctat agcggtaact
     1201 gactggaata aaaaatatag taaaaatgag atttataaac gtataaatca attcgctaaa
     1261 aatgagaacg tagcaatcta taaatcaact tcaaattata caaacaaaaa tgtagataaa
     1321 gat
//
LOCUS       DAHHRR010000244         1314 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.250, whole genome shotgun sequence.
ACCESSION   DAHHRR010000244 DAHHRR010000000
VERSION     DAHHRR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.250"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..1303
                     /locus_tag="NBI42_002714"
     CDS             <1..1303
                     /locus_tag="NBI42_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCD1779051.1"
                     /translation="FYSKRQLSKHLSISQTTVEHAYQLLLDEGYIYSRPRSGYYVSEI
                     ESLTILNTQPIHSLFDDDSYKPKTSDENYDYAFNLDEIDTKHFPIELFRKYSKDLYDK
                     THSNKLLRGHFQGELHLRFQLAFYLFTNRGVICDPNQIIIGSSTEQLVNQLVDLLYTS
                     TFIIEKPSYPPIKNILDKKQVEYEQIEVEDNGINVDEVIKSQKNIVYITPSHQFPTGY
                     VMDLKKRTQLIQWAQEKEERFIIEDDYDSEFRYFGKPIPAIQGLYSRGEKVIYISTFS
                     KSIFPSCRVAYMVLPYSIMKKYHSQNHIEGNTVPVHMQNLIATFISSGGFERHLNKMR
                     RIYRRKLTYILNRLKPYKEQLDIQGAETGMHFTITVKNGLTLQECLDRANKVKLKLQV
                     YNFDDDQDYKKTPKFILGFGGIDDDALKPHVDALIKSLIIK"
BASE COUNT          499 a          200 c          195 g          420 t
ORIGIN      
        1 gttttattca aagaggcaat taagcaaaca tttatctatt agccaaacaa cagtagagca
       61 tgcttatcaa ctattattag acgagggata catatattcg agacctcgct ctggttatta
      121 tgtgtctgaa attgaatctt taacaatttt aaatactcaa cctatccatt ctctttttga
      181 tgatgattca tataaaccaa aaactagtga tgaaaattat gattacgctt ttaatttaga
      241 tgaaatagac acgaagcatt tcccaataga acttttcaga aagtattcaa aagatcttta
      301 tgataaaacc cattcaaata aattacttag aggccatttt caaggagaat tacatttgcg
      361 gtttcaactg gcattttatt tatttactaa tcgtggcgta atttgtgatc ctaatcaaat
      421 tataattggc tcatctaccg aacaattagt aaatcaatta gtcgatttac tttatacttc
      481 aacgtttatt atcgaaaaac ctagttatcc accaatcaaa aatattttag ataaaaaaca
      541 agttgaatat gaacaaattg aagttgaaga taatggaata aacgtagatg aagttattaa
      601 atcacaaaaa aatatagtat atataacgcc ctcccatcaa tttccaactg gatatgtaat
      661 ggacttaaaa aagcgaactc aattaataca atgggctcaa gaaaaagaag aacgatttat
      721 tatagaagat gattatgatt ccgagtttag atatttcggt aaacccattc cagcaattca
      781 aggtctatac tcaagaggag aaaaagtgat ttatatcagt acattttcaa agtctatttt
      841 tcctagctgt cgtgttgcat atatggtgtt accctattct ataatgaaaa aatatcattc
      901 tcaaaatcac attgaaggca acactgtacc tgttcatatg caaaacctta ttgcaacgtt
      961 tatatcttcc ggtggttttg aaaggcattt aaataaaatg agacgaattt atcgtagaaa
     1021 actaacctat attttaaata gattgaagcc ttacaaagag cagcttgata ttcaaggggc
     1081 tgaaacaggt atgcatttta caattacagt aaaaaacgga ttaacattac aagagtgtct
     1141 tgatagagca aataaagtaa agttaaaatt acaagtttat aattttgatg atgaccaaga
     1201 ctataagaaa acccctaaat ttatattagg ctttggcggt atagacgatg atgctttaaa
     1261 accgcatgta gacgctctaa taaaatcatt aattataaag taacacttct ttga
//
LOCUS       DAHHRR010000245         1304 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.251, whole genome shotgun sequence.
ACCESSION   DAHHRR010000245 DAHHRR010000000
VERSION     DAHHRR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.251"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..554)
                     /gene="bshA"
                     /locus_tag="NBI42_002715"
     CDS             complement(<1..554)
                     /gene="bshA"
                     /locus_tag="NBI42_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HCD1779052.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTA"
     gene            complement(809..1126)
                     /locus_tag="NBI42_002716"
     CDS             complement(809..1126)
                     /locus_tag="NBI42_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCD1779053.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
BASE COUNT          402 a          229 c          158 g          515 t
ORIGIN      
        1 gctgtgttat gtttagttgg aaactcattt tcacgtacaa aattataaat cggtataatc
       61 tctttgttag tttcaataat ttcatgtgtt tcttgcgcta aagatttact cacacttgtc
      121 acaatatcac ttttttcaat gccaaattta attgcacctt ggagtgaatg atcatagccc
      181 aaaacagtaa tatcagtacc gtgtaacgtt gtcataattt ttatatcttt acctgacatc
      241 tcacgagcta aaattccaca aattgcatga ggtacagcat agtgcatatg caacaaatca
      301 agatcatatt ctttaataac ttcagcgatt ttagtactta acgtaatatc atacggtgga
      361 tattgaaata ctgcatattg attcacttca acttgatgaa aaatcatatt cggtaatggt
      421 tttcttattc taaacgggat atttgaagtg ataaaatgta cttcgtgacc tcgctctgct
      481 aatttaattc ctaattctgt ggcaataatt ccagaaccac ccatggacgg gtaacatgtt
      541 atacctatct tcattcgctt gcccatcctt tctttctatt tctctttcta ttattctcga
      601 tgcatagata attgtttaaa tttaatttta caatttaagt ttatagtaat gttgagttta
      661 taatttcaaa tatctaaaaa acaggtgtta tatatattca tttatctagt tatactcact
      721 ttaaaaataa aatgatttca tgcgatgcaa ttcattggtc tatgttttta atcttaatca
      781 aatccaaata aagcaaatat ttttatattc actttctttc gaatcgattt ttatctcttg
      841 tattaaactt ttccattgtt tcattaaagc tctctgtcat atctattccc attgaattcg
      901 ctaaacataa caacacaaat aaattatcac ctaattctgc tttaatcgta tttgcttcct
      961 ctgaatcttt cttcttcttt tcaccatagg tatgatttat ttcacgtgca agttcgccca
     1021 cttcttcagt caatctagct aagttagcta atggtgagaa atatccagtt ttaaattgtc
     1081 caatatattc atcaacttca cgttgcattt ctaccattga tttcatttct acgttctcct
     1141 tatattgcat ttctaatata gtatatatca atttgaagtc tcatgcatgt ttagtgcaat
     1201 ctactgaatc tagcttaact agatgacaag ttgtttattt tagtagttta ataattcagt
     1261 ttatataaat gtaatgcatt cctaactaaa ttaaatcaat tgaa
//
LOCUS       DAHHRR010000246         1299 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.252, whole genome shotgun sequence.
ACCESSION   DAHHRR010000246 DAHHRR010000000
VERSION     DAHHRR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.252"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>1299
                     /locus_tag="NBI42_002717"
     rRNA            <1..>1299
                     /locus_tag="NBI42_002717"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          337 a          292 c          392 g          278 t
ORIGIN      
        1 acactgccga gaaaagcctc tagatagaaa ataggtgccc gtaccgcaaa ccgacacagg
       61 tagtcaagat gagaattcta aggtgagcga gcgaactctc gttaaggaac tcggcaaaat
      121 gaccccgtaa cttcgggaga aggggtgctc tttagggtta acgcccagaa gagccgcagt
      181 gaataggccc aagcgactgt ttatcaaaaa cacaggtctc tgctaaaccg taaggtgatg
      241 tataggggct gacgcctgcc cggtgctgga aggttaagag gagtggttag cttctgcgaa
      301 gctacgaatc gaagccccag taaacggcgg ccgtaactat aacggtccta aggtagcgaa
      361 attccttgtc gggtaagttc cgacccgcac gaaaggcgta acgatttggg cactgtctca
      421 acgagagact cggtgaaatc atagtacctg tgaagatgca ggttacccgc gacaggacgg
      481 aaagaccccg tggagcttta ctgtagcctg atattgaaat tcggcacagc ttgtacagga
      541 taggtaggag cctttgaaac gtgagcgcta gcttacgtgg aggcgctggt gggatactac
      601 cctagctgtg ttggctttct aacccgcacc acttatcgtg gtgggagaca gtgtcaggcg
      661 ggcagtttga ctggggcggt cgcctcctaa aaggtaacgg aggcgctcaa aggttccctc
      721 agaatggttg gaaatcattc atagagtgta aaggcataag ggagcttgac tgcgagacct
      781 acaagtcgag cagggtcgaa agacggactt agtgatccgg tggttccgca tggaagggcc
      841 atcgctcaac ggataaaagc taccccgggg ataacaggct tatctccccc aagagttcac
      901 atcgacgggg aggtttggca cctcgatgtc ggctcatcgc atcctggggc tgtagtcggt
      961 cccaagggtt gggctgttcg cccattaaag cggtacgcga gctgggttca gaacgtcgtg
     1021 agacagttcg gtccctatcc gtcgtgggcg taggaaattt gagaggagct gtccttagta
     1081 cgagaggacc gggatggaca tacctctggt gtaccagttg tcgtgccaac ggcatagctg
     1141 ggtagctatg tgtggacggg ataagtgctg aaagcatcta agcatgaagc ccccctcaag
     1201 atgagatttc ccaacttcgg ttataagatc cctcaaagat gatgaggtta ataggttcga
     1261 ggtggaagca tggtgacatg tggagctgac gaatactaa
//
LOCUS       DAHHRR010000247         1233 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.253, whole genome shotgun sequence.
ACCESSION   DAHHRR010000247 DAHHRR010000000
VERSION     DAHHRR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.253"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>1233
                     /locus_tag="NBI42_002718"
     CDS             <1..>1233
                     /locus_tag="NBI42_002718"
                     /inference="COORDINATES: protein motif:HMM:NF024565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1779054.1"
                     /translation="KAKFLKELTNPRVSKTNYNRDDLQREISSAKKLETEEVKKCEKI
                     LKTEDKEEIDNYELLDFNFEKLHNQVLGIINYELNEIVSFEELNKPNKKEFAEKGLSL
                     HNPGDKCLFCGNIVENERYFKLEKYFKVDEINDLRSKVNILIGELDNVKNSIKSLQKN
                     DEIYFEHLREDLSLVEEKYKSVLLEERNYIDNLIGGLKEKCRTPYSNDIAMPNLIESQ
                     YITYIKEHNKIIEKHNLFSKNIKYEQEQAEKRLRYHYISVIIEEKKQYNENWKGYIIE
                     NTILQENYKKLEKSLKDLQDEIKKLEGNDEQNETDNLKSIKNNINKLSIERSRLLESV
                     QSTEKWVNIINDKLKKIGKTNLKLCLEKNEDNVEHYVIHDDTNGTREITKLSTGEKNI
                     ISFLYFMESLVDIRNDFKK"
BASE COUNT          580 a          107 c          200 g          346 t
ORIGIN      
        1 aaagcaaaat ttttaaagga attgacaaat ccacgtgtct ctaaaacaaa ttataataga
       61 gatgatctgc aaagagaaat ttcttccgca aaaaaattag aaacagaaga agtgaagaaa
      121 tgtgaaaaaa tattaaaaac agaggacaag gaagagatag acaattatga attattggat
      181 tttaattttg agaaacttca taatcaggtc cttggaatta tcaattatga attaaatgaa
      241 attgtatcat ttgaagaatt gaataaacct aataaaaaag agtttgctga gaaaggactc
      301 tctctccata atccaggaga caagtgtcta ttttgtggaa atatagttga aaatgagaga
      361 tattttaaat tagaaaaata ctttaaggtt gatgaaatta atgacttaag aagcaaagta
      421 aatattttaa taggagaatt ggataatgtt aaaaattcaa ttaaaagtct gcaaaagaat
      481 gacgaaatct atttcgaaca ccttagagaa gatttgagtt tagttgaaga aaaatataaa
      541 tcagtgcttc tagaagaaag aaactatatt gataatttaa taggaggact aaaagaaaag
      601 tgtagaactc catatagtaa tgatattgct atgcctaatt taattgaaag tcaatatatt
      661 acttatatta aagaacataa taaaataata gaaaaacata atttattttc gaaaaatatt
      721 aaatatgaac aggaacaagc tgagaaaagg ctaagatacc actatatcag tgtgataata
      781 gaggaaaaaa aacaatataa tgaaaattgg aaaggatata taattgaaaa tacgatttta
      841 caagaaaatt ataaaaaatt agaaaaatca ttgaaagatt tgcaagatga aataaaaaag
      901 ttagagggaa atgatgaaca aaatgaaacc gataacttga aaagcattaa aaataatatt
      961 aataagttgt ctatagagag aagtagatta ttagaatctg tacaatcaac tgaaaaatgg
     1021 gtaaacatta taaatgacaa actaaaaaaa ataggaaaaa caaatttgaa actctgtctt
     1081 gagaaaaatg aggataacgt tgaacattat gtaatacacg atgatactaa tggtactaga
     1141 gaaataacta agttatcaac aggagaaaaa aatattatat catttttata ttttatggaa
     1201 agcttggttg atataagaaa tgattttaaa aaa
//
LOCUS       DAHHRR010000248         1225 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.254, whole genome shotgun sequence.
ACCESSION   DAHHRR010000248 DAHHRR010000000
VERSION     DAHHRR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.254"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..917
                     /locus_tag="NBI42_002719"
     CDS             <1..917
                     /locus_tag="NBI42_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HCD1779055.1"
                     /translation="YKSYERLYKKPTNSRMLKEKIEDLESIIDEFFEEINKERPKDIP
                     VLSEYEHENKEKAITLKSDTEAAKNDETKRSKRSKRSLNTQNHKSASQEVSEQQKAEY
                     EKRAEERKARFLDKQKNKQTPLMSLEYDFEHKQRVDNENDKQLVVSAPTKKPTPPPTY
                     TETTTQVPMPAVERQAQQQIVYKTPKPLAGLNGESHDFTTTHQSPTTSNHTHNNVVEF
                     EETSALPGRKSGSLVGLSQIDSSHLTEREKRVIKREHVREAQKLVDNYKDTHSYKDRL
                     NAQQKVNTLSEGHQKRFNKQINKVYNGK"
BASE COUNT          527 a          187 c          194 g          317 t
ORIGIN      
        1 gctataaaag ttacgaaaga ttgtataaaa agcctactaa ttcaagaatg ttaaaagaga
       61 aaattgaaga tttagagtca ataatagatg aatttttcga agaaataaat aaagaaaggc
      121 ccaaagatat ccctgttttg tctgaatatg aacatgaaaa taaggaaaag gctataactt
      181 taaaatccga cactgaagct gctaaaaatg acgaaacaaa aagaagcaag agaagtaaaa
      241 gaagtttaaa tactcaaaat cacaaatctg catctcaaga agtctctgaa caacaaaaag
      301 ctgaatatga gaaaagagca gaagaaagaa aagcgagatt tttggataag caaaaaaata
      361 agcaaacgcc tttaatgtca ttagaatatg attttgaaca taaacaacgt gttgacaacg
      421 aaaacgacaa gcaacttgtt gtttcggcgc caacaaagaa accaacacca ccaccgacat
      481 atactgaaac gacgacacag gttccaatgc cagcagtaga gcgtcaagca cagcaacaaa
      541 tcgtttacaa aacaccaaaa ccattggctg gattaaatgg tgaaagtcat gatttcacaa
      601 caacgcatca atcaccaaca acttcaaatc acacgcataa taatgttgtt gaatttgaag
      661 aaacgtctgc tttacctggt agaaaatcag gatcactggt tggtttgagt caaattgatt
      721 cttctcatct aactgaacgt gagaagcgtg taattaagcg tgaacacgtt agagaagctc
      781 aaaagttagt tgataattat aaagatacac atagttataa agaccgatta aatgcacaac
      841 aaaaagtaaa tactttaagt gaaggtcatc aaaaacgttt taataaacaa atcaataaag
      901 tatataatgg caaataatta atgcatggct gcaaagcaaa taatgagttt gccgtaaaaa
      961 taacaacatt ttaaactagc aataaataat atcaaagtca tcatttcaat gatgcaatct
     1021 agtatagtcc acattctaaa caggtgtgga ctattacttt ttttacttta tattacgaaa
     1081 aaattattat gcttaactat caatatcaat aattaatttt aagctgaaaa acaataaaaa
     1141 tgttaagaca acgtttactt caagttaatt attatactga aaattctggt atataatgct
     1201 gttagtgaat ataacaggaa aatta
//
LOCUS       DAHHRR010000249         1222 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.255, whole genome shotgun sequence.
ACCESSION   DAHHRR010000249 DAHHRR010000000
VERSION     DAHHRR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.255"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            588..773
                     /locus_tag="NBI42_002720"
     CDS             588..773
                     /locus_tag="NBI42_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779056.1"
                     /translation="MSIGKFTQKVKGKNPTWRDPKTKWTISKNKGQPHGGSYWKLINN
                     KGKRIASLTKEGKILRE"
     gene            787..>1222
                     /locus_tag="NBI42_002721"
     CDS             787..>1222
                     /locus_tag="NBI42_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779057.1"
                     /translation="MSFDINNLDIKKLNFRKVKNAIHLSLSGDKYQLLEDGKTINNTY
                     FLAVYDNAINIFEDLFSPSDSINLVHVVYVYNEPYKKTNVIKKFTSLTIDELHYFKEY
                     KKEKDDNILCEEFSYKCSIKDIKYKKLLKAISNQDFKGLVPNI"
BASE COUNT          495 a          133 c          214 g          380 t
ORIGIN      
        1 tgatgtaaaa aggaggggat tacatgaagt ttaaaatttt taagtcgttt atatgtttag
       61 ttacatttgc tgttttacta tctggatttg gaaatataca agcgcaagaa gagaatgttt
      121 tgattaaaga aaattcccaa gttgccgatt ctatttctca tgcagatgtt actgaaagca
      181 agactggaga aatatccaca gttaatactt ctattaaaga tgaagaggtg tctttaaaca
      241 caaccctaac aaacaatttt gaaacgggtg aaattaattt aaatggtaaa ataggcaata
      301 ataaagaaga aaaaaacata aattattccg ttgatttaac atatgcagat gagaatgata
      361 ttgcaggata tttaattgat aataaaactg gagagaaaca tccgtttgat actcgtttat
      421 ctgaagcttc agcagcacct ttagttgtag tagcagtaat ggctgctcga tttggcataa
      481 aatgggctat taaaaaatat ggcaaaaacg ttgtaaataa agcagtaaag actaatactc
      541 ataatatagc caaaaacgtt aataaaagtt tattaaatga caagggtgtg agtattggca
      601 aatttactca aaaggttaaa gggaaaaatc ctacctggag agatccaaaa actaaatgga
      661 ctatttcaaa aaataaaggg caaccacatg gtggttctta ttggaagtta ataaataaca
      721 aaggaaaaag aatagcttct ttaactaaag aaggaaaaat cttaagggaa taaggtgttg
      781 taaattatga gctttgatat aaataatttg gatataaaaa agttgaattt tagaaaggtg
      841 aaaaacgcaa ttcatttaag tttaagtggc gataaatacc agttattgga ggacggtaaa
      901 actatcaata atacttattt tttagctgta tatgataatg caattaacat ttttgaagat
      961 ttgttttcgc cttctgattc tataaattta gttcatgtag tatatgttta taatgaacct
     1021 tataagaaaa caaatgttat aaaaaaattt acatcgttaa caatagatga attgcattac
     1081 tttaaagaat ataaaaagga gaaggatgat aatattttgt gcgaggagtt ttcatataag
     1141 tgttccataa aagatataaa atataaaaaa ttattaaaag ctatatccaa tcaagacttt
     1201 aaagggttag ttccaaatat ca
//
LOCUS       DAHHRR010000250         1183 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.256, whole genome shotgun sequence.
ACCESSION   DAHHRR010000250 DAHHRR010000000
VERSION     DAHHRR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.256"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..164)
                     /locus_tag="NBI42_002722"
     CDS             complement(<1..164)
                     /locus_tag="NBI42_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779058.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSG"
     gene            complement(193..1002)
                     /locus_tag="NBI42_002723"
     CDS             complement(193..1002)
                     /locus_tag="NBI42_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1779059.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNCEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSSKEIPFTTTKELNGNK
                     YFSFEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKIGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
BASE COUNT          357 a          209 c          168 g          449 t
ORIGIN      
        1 aatcctgaat tatctattat ttgataatat tcttcagttt cgttgataac atcatattgt
       61 ttgcctacag ttaacatgtc agaaactgta aatttctttg catctgtatt aatgacttct
      121 actttcttga ttggtgtacg ttctttccaa gtttcatgta acataaattt tgtcctcact
      181 atcgtaaaaa aattattttt gtaaagtgaa tataaacgat aatccactgt tttcagtaat
      241 tctaattgat tcaatatcat cttgctcaag cgtttgatat ggtatttcca cttcatctaa
      301 gcgcgatttc gcttgattaa agaaatctgc atctttcgtt tcaaactcaa cttgatcgac
      361 tgcgccatat tccggcattg tcatttcggt ttctacaggc attaaatgta tttcgccacc
      421 tagaccaccc gttccaattt tgaatacttg gacatgatag tcagcattgt cgaagggttg
      481 gtattctgca aatacttcaa gtccgaatat atttgttaaa atttgacctg taatatctac
      541 atgattcact ttaagaatca ctggccctaa accttgcact tgatgtaacg gattgacagc
      601 actttcgaaa gaaggcatgc ctaaaccgac gccataatta ttctcatttg agtaaattga
      661 gaaaatatga ccgttattat cttcaaaact aaaatattta ttaccattta gctctttagt
      721 tgttgtaaat ggaatttctt tactcgataa tatttccgca tactcctcta aacctgagtc
      781 agttggtgta cgtaatccaa taccaacaaa gtgagattct tctaattgct cacttggaac
      841 ttgtataaat tgaagtcttg ttcctgggct tatatctgca tcgccgaaac gaattgagtt
      901 ttcaagaagt tcttcacaat ttaatcctaa tatgtcaacc atgaaatgtt ttgtctgttc
      961 tatatttgtt gtacctaatg ttatacttct aagtccacac attatagcca cctctatctt
     1021 tatctttact attatattat aacttatttt aatcataaat tatagaatat cggtcagttg
     1081 attttgatta tagattaata tgtgaaaaat gtatttcttt aaaagaacta tttcatttaa
     1141 ataggtaaca taatatagtt atggttgcta tcgagtatgt cat
//
LOCUS       DAHHRR010000251         1169 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.257, whole genome shotgun sequence.
ACCESSION   DAHHRR010000251 DAHHRR010000000
VERSION     DAHHRR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.257"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..842
                     /locus_tag="NBI42_002724"
     CDS             <1..842
                     /locus_tag="NBI42_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin biosynthesis cyclodehydratase"
                     /protein_id="HCD1779060.1"
                     /translation="IAIDKEESSKNFIVKFKTLIAENQIELNENDDVIDDFNKLTQLN
                     LIHKKIDTFNKICIITDKVAKAFFKDHLDQVDEIYEFNEVLERHEIETLNNQKSNLSI
                     NEVVKKQQSKLANMNVFVILSYANQPFIKAFNFLTNLLDITYTLVFYDNENVFFTNIQ
                     HGETGCYECLERQLVSKFPGKIENYLVPDEQNQGIYNLKLYSYILSIMINEINNIEIY
                     GDSTLIGNVLHFYLPTYEYNFNFNKRQSTCSTCSTINNVLFKEQNIKSANILKRMLEN
                     DNL"
     gene            829..>1169
                     /locus_tag="NBI42_002725"
     CDS             829..>1169
                     /locus_tag="NBI42_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagD"
                     /protein_id="HCD1779061.1"
                     /translation="MITFKNNLNYNLYRQNLLGGNVAGIWKDNSFFLGLNSYPIMNYH
                     YVTAQFNNFEKNFLSEDFKVNYHLSGYGIHYNEALASFLGESSERYAFTLLYQNLKKK
                     IIYASHAQISE"
BASE COUNT          481 a          132 c          165 g          391 t
ORIGIN      
        1 caatagctat tgataaagaa gaaagcagta aaaattttat agtcaaattc aaaacgttaa
       61 ttgctgaaaa tcaaatagaa ctaaatgaaa atgacgatgt tatagacgat tttaataaac
      121 taacacaatt gaatttaata cataaaaaaa tcgatacttt taataaaatt tgtattatta
      181 cagacaaggt ggccaaagca ttttttaaag accatctcga tcaagttgat gaaatatatg
      241 agtttaatga agttctagaa agacatgaaa ttgaaacctt gaataatcaa aaaagtaatt
      301 tatctataaa tgaagttgta aaaaaacagc aatcaaagtt agcaaatatg aatgtttttg
      361 ttattttatc atacgccaat caacctttta ttaaagcgtt taacttttta acaaacttgt
      421 tggatataac ttatacactt gttttttatg ataatgaaaa tgtatttttc acaaatattc
      481 aacatggaga gacagggtgt tatgaatgtt tagaacgaca attagtttct aagtttcctg
      541 gtaagataga aaattattta gtaccggatg agcaaaatca aggtatatac aatctcaaat
      601 tatacagtta tatattaagt ataatgataa atgaaattaa taatatagaa atttatgggg
      661 attcaacact aataggaaat gtacttcatt tttatttacc cacatatgaa tacaacttta
      721 atttcaataa gagacagtct acttgttcga catgttcgac gataaataat gttttgttta
      781 aagagcaaaa tataaagtca gctaatattt taaaaaggat gttagaaaat gataaccttt
      841 aaaaataatt tgaattataa tctgtataga caaaacttat taggcggaaa tgtagcaggt
      901 atttggaaag ataacagctt tttcttaggt ttaaattcat atccaattat gaattatcat
      961 tatgtaacag cccaatttaa taattttgaa aaaaactttt taagtgaaga tttcaaagtg
     1021 aattatcatt taagtggtta tggcattcat tataatgaag cactagcgtc attcttagga
     1081 gaaagttcag aaagatatgc ttttacgctg ttatatcaaa atttaaaaaa aaaaatcata
     1141 tatgcgtcac atgcacaaat ttcagaaca
//
LOCUS       DAHHRR010000252         1134 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.258, whole genome shotgun sequence.
ACCESSION   DAHHRR010000252 DAHHRR010000000
VERSION     DAHHRR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.258"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            355..>1134
                     /locus_tag="NBI42_002726"
     CDS             355..>1134
                     /locus_tag="NBI42_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1779062.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVAL"
BASE COUNT          433 a          166 c          158 g          377 t
ORIGIN      
        1 gtaaagatgc attaatcgag ttataatcat aatgattaag atggtagatt tagtcttata
       61 taactacatt tactaagatt taaatagatg agtacataat gtataaattg ggaatataca
      121 gtatgactta cggtaatata tcaatatatt gatatattat ttgcacatgg gagtatgcta
      181 ttttttatct atacatctta aaaaaattta cagttttata attcaatatc attaaacaaa
      241 caacatattc agcttatttt tcattctctt acgttacaag ttgtaaatac catcgcgtta
      301 aacaacatta gccaacgcta ataaaatata ttgcgttaaa gtggagtgat taatatgtca
      361 aacgtactaa cagaaataga tagtcaatat ccctatatga ctaaaaatga aaaaaagata
      421 gctaaattta tactaaattc acctcaaaag gtgattaaaa tgaggtctca agacttagca
      481 agcttattag atattagtac atcatcagtc attcgattca gcaagaaaat tactgatggc
      541 ggttttcacg acttgaaaat aaatatatct aaatatgttc ctaaagcatc atcaatttac
      601 aatgttgaat taatgaataa cgaaagcaca gaatctttaa gaactaaact ccatacacgc
      661 actacacgtg cacttaatca tgcaaataac gaattaaatg ataaaactat tgatcaaata
      721 tgtcattgtt taaaacgttc tgagacgatt tttatatatg gttttggcgc atcttttgta
      781 gttgctaccg acttatacca aaagttatca agaataggtc ttaatattca acttgttcaa
      841 gaaacacata tttttgcaac attattagca actcacaatt cgaacgacag tgttattctt
      901 attactaaca atggtacgca aagtgaaatg caatcaatgg ttaaagttat tgatgactac
      961 catataccta taattacaat tactagtaca agggataatc ccgtagcaca ggcatcaaat
     1021 attgttttaa cttatgggaa aactgatgaa aatgagatgc atatgggagc aacaacttca
     1081 ttatttgctc aaatgttcac tattgatatt ttatattatc gttatgttgc atta
//
LOCUS       DAHHRR010000253         1121 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.259, whole genome shotgun sequence.
ACCESSION   DAHHRR010000253 DAHHRR010000000
VERSION     DAHHRR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.259"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..365)
                     /locus_tag="NBI42_002727"
     CDS             complement(<1..365)
                     /locus_tag="NBI42_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779063.1"
                     /translation="MSNLHLSSIQLIFDDMRDKDYIIDSFKFKYKNENYLVFIRRFKG
                     KFIKRINKFAKVQLTFYKESTMKNSLVVEANNNGLIVNDYNMLYNFFGIESSSFGKGL
                     FPFYESLNEAIPNEVNLNS"
     gene            complement(688..1121)
                     /locus_tag="NBI42_002728"
                     /pseudo
     CDS             complement(688..1121)
                     /locus_tag="NBI42_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592003.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          389 a          181 c          103 g          448 t
ORIGIN      
        1 cttgaattaa gatttacttc gttaggaatt gcttcattaa gactctcata aaaaggaaat
       61 agacctttac caaaactaga tgattcgatg ccaaaaaaat tatatagcat attgtaatca
      121 tttactatta atccattatt atttgcttca actaccaagg aatttttcat agttgactct
      181 ttataaaatg ttaattgaac tttcgcgaat ttatttattc ttttaataaa ttttcctttg
      241 aatctgcgga taaagactaa ataattttca tttttatatt taaatttaaa tgaatcaata
      301 atataatcct tatcacgcat atcgtcaaat attaattgta tactggacaa atgcaaattg
      361 ctcatggtta tctactcctt ataaaaatgg aattctacac aacaattatc ctaacaactc
      421 tatggtcggc attatccctc acataaatgt ccctcataaa ttgcataggt tgatatcgac
      481 tttaatttac tctatttctt tatcattaaa tacactttaa cacatttgta aaatcataat
      541 cattcactct aacatagtag aattcataat ttttcttaag tatattaatt gtatattttc
      601 atattaaagt ccaatgaata tactttaatc atgcatttat tagtcgaaat ttttatattt
      661 cattaactaa ctgcctactt aaattattta atctctattc ttactcatca tttcaaagtg
      721 ttggatatgt cctagttttt caaagactac cttaaagact cttttataat ttctcatact
      781 tgcttcatca aaggtaataa caaaatcatc acttataaaa tgtgatccgt catttattcc
      841 tgaaataagt gatttacata atattttgtc tataccgtca aatttattaa tacatttttc
      901 ataatccatg ccccctataa tattaaaata atattatagt attctacgca tagtattaaa
      961 tgatgttatt ggtgacattt cttcaggatt aagttctttc cacattaatt gatatgaaga
     1021 ttctatagga ttattctcat aagtattaaa ataacatata ttatcctttt ttctaataat
     1081 tccatacatt acttcattag gcgaaaacct ttctctactt c
//
LOCUS       DAHHRR010000254         1096 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.260, whole genome shotgun sequence.
ACCESSION   DAHHRR010000254 DAHHRR010000000
VERSION     DAHHRR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.260"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..880
                     /locus_tag="NBI42_002729"
     CDS             <1..880
                     /locus_tag="NBI42_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1779064.1"
                     /translation="PNKRKVNTVFQDYALFPHLNVYDNIAFGLKLKKLSKTEIDQKVT
                     EALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDESLSALDLKLRTEMQ
                     YELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGTPTDIYDEPVNRFVA
                     DFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIRPEDISLIKAEEGLF
                     KATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYFDPEAIHIMVPGETE
                     EEFDKRIESYEEVDNA"
     gene            873..>1096
                     /locus_tag="NBI42_002730"
     CDS             873..>1096
                     /locus_tag="NBI42_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1779065.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAALITIITLAIS"
BASE COUNT          399 a          134 c          198 g          365 t
ORIGIN      
        1 accaaataaa cgtaaagtga atacagtctt tcaagattat gcattatttc cacacttaaa
       61 cgtatatgat aatatcgctt tcggtttgaa attaaaaaaa ttatcaaaaa ccgaaattga
      121 tcaaaaagta actgaggcat taaaattagt taagctttca ggttatgaaa aaaggaatat
      181 taatgaaatg agtggcggac aaaagcaacg tgttgcaatt gcacgtgcta tcgtaaatga
      241 accagaaata ttattgttag atgaatcttt atccgcattg gatttgaaat tgcgtactga
      301 aatgcaatat gaattaagag aattgcaatc tagattaggt attacattta tatttgtaac
      361 gcatgatcaa gaagaagcgt tagcactaag tgactttctt tttgtattaa aagatgggaa
      421 aattcaacaa tttggtacac ccacagatat atatgacgaa ccagtgaatc gatttgtagc
      481 tgattttatt ggagaatcta atattgttga agggcgaatg gttagagatt atgtcgtgaa
      541 tatttatgga caagatttcg aatgtgtcga tatgggtatt cctgaaaata aaaaagtaga
      601 agtcgttatt cgaccagaag atatatcatt aatcaaagct gaagaaggat tatttaaagc
      661 aactgttgat tctatgttat ttagaggggt ccactatgaa atatgttgta tagacaataa
      721 aggttatgaa tgggtaatac aaacgactaa aaaagctgaa gtaggcagtg aagttggcct
      781 ttattttgat cctgaagcca ttcatattat ggttcctgga gaaacagaag aagaatttga
      841 taaacgtatt gaaagctatg aggaagtaga caatgcgtaa tactaataaa tttctcttaa
      901 tcccgtattt actatggatg gttatattta ttattgtacc agttgtatta ctcatttatt
      961 tttcattttt agatatcaat ggacatttta gtttcacgaa ttatcaacaa atttttacta
     1021 caaaatattt aaaaatgttt gcatattcaa ttttatatgc cgctttaata acaattatta
     1081 cgttggctat cagtta
//
LOCUS       DAHHRR010000255         1069 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.261, whole genome shotgun sequence.
ACCESSION   DAHHRR010000255 DAHHRR010000000
VERSION     DAHHRR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.261"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
BASE COUNT          461 a          177 c          119 g          312 t
ORIGIN      
        1 aattagaaag atagtaagca aaatttggaa attttcgata gagtctaatt aaattatttg
       61 gtgcataatt aatatagatt tcaaacctat gtatttcaaa aaacaataca tagccatttt
      121 gaactaactt gctaatcttt tattgatatt caactatgtt ataaaaaaag caaattgacc
      181 aaaatggaag tttgaatcaa aaaacattaa atgtacccat gacttaaatt ttatcgccat
      241 tatttaagtt tggacataca cttttgtcat caaacgctga aaattagtac gattgaaatc
      301 acttcttgtt ataaaaattc ttagtagccc tgttacaaaa tattcgctac aaattattca
      361 aatcaacaac aataaccatc aactaagaaa ttttaaacaa tctactgaaa aataaagcgt
      421 ttaaaagttc tgtttagaaa acatcaagat aagtataaac atctagaaaa gtgaacatct
      481 aagaaaatcc atctagttat tgccgtaaca attagaataa tcatttgaaa aagtgcatta
      541 gacaacgaaa ttcaccatca agataatgaa gcaacaagaa acagcacaca tcaatgaatt
      601 aacaccttag attcatcatg agaaaatgca tggcacaact agatttacca tcaagataat
      661 tcttaagcag cgaagaatta acatctagaa atgcataaac atcttagatt ttacatcaag
      721 agatgagata actataaaca actagaaacg tcaccaagat agtgcaacaa caaagaatca
      781 gcatcttgaa atcgcactaa catctaagaa tcaacatcaa gtattatatt aacatcatag
      841 ataacttcat caagacaggc atccacatct aagaaactca tctagaatag ctaacaccta
      901 acatcaaaga atattcatca agaacagtat ctaacagaac tttttgacta tatatgaatt
      961 aattatgtta ctagctaaat catctattat taactttcac actcaatttc tacatctaat
     1021 atcaatttat taacaataga attagcagca acaagatatt acttaaaat
//
LOCUS       DAHHRR010000256         1064 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.262, whole genome shotgun sequence.
ACCESSION   DAHHRR010000256 DAHHRR010000000
VERSION     DAHHRR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.262"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            119..997
                     /locus_tag="NBI42_002731"
     CDS             119..997
                     /locus_tag="NBI42_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HCD1779066.1"
                     /translation="MINYQLKGMFNQHSLFKLNDDNIANNDNRNNFAQIILGNQESES
                     LKYLLNVNHQRLSDFKSMLAYNETNYAALVNEHQILNEINDEDTRSLPKSDKKIKESF
                     YNVLNHRKSTRKFTSFEMACKELSTILKFSFGIAPRKVEYGDIKTSTRFYASAGGLYP
                     IDIYLYINNVEDYDKGIYLYQPISHSLLKIVAQKDINTDDILEGDNIDIENCNFLVFF
                     GYNFNSSFLKYGELALLNSLIEIGCMSHNFDLVITSLGYTGCPIAGFKKNNIEKLLEL
                     DHVNEHILFTNICGKE"
BASE COUNT          411 a          114 c          178 g          361 t
ORIGIN      
        1 atggcgttag tggagagcca attataacaa atattatgtt tttaggtgtg atcttatcca
       61 tattattagt gattattatt gaattgcttg tagcaactaa caaaagaggt aaaaagcaat
      121 gattaattat cagttgaaag gaatgtttaa tcagcattcg ttatttaaat taaatgacga
      181 taatattgca aataatgata atcgaaataa ctttgctcaa attattttag gaaatcaaga
      241 atcagaatct ttaaagtact tattaaatgt aaatcatcag agacttagtg acttcaaaag
      301 catgttagct tataatgaaa cgaattatgc agctttagtt aatgaacatc aaattttgaa
      361 tgaaattaat gatgaagata caaggtcact tcctaaaagt gataaaaaaa taaaagaatc
      421 tttttataat gttttaaatc acagaaaaag tacaaggaaa ttcacatctt ttgaaatggc
      481 ttgtaaagaa ttgtcaacaa tacttaaatt ttcttttggt atagcaccta gaaaagtgga
      541 atatggggat ataaagacaa gtacgcgatt ctatgcatcg gcaggtgggt tatatccgat
      601 tgatatatat ctttatatca acaatgtaga agattatgat aaaggtattt atttatacca
      661 accgataagt cactcattat taaaaattgt ggctcaaaag gacataaaca cagatgacat
      721 tttagaaggt gataatatag atatcgaaaa ttgtaatttt ttagtctttt ttggctataa
      781 ttttaattct agttttttga aatacggtga attagcgtta ttaaatagtt tgatagaaat
      841 cggttgtatg tcacataatt ttgatttagt cattacgtca ttaggctata caggatgccc
      901 aatagctggc tttaagaaaa ataatataga aaaattactt gaactagatc atgttaatga
      961 acatatacta tttacgaata tttgtgggaa ggaatagttg agcaatgaaa acgtttatta
     1021 aacctaaaga tttgtttgtc gaagaaaaca atagtgagta tata
//
LOCUS       DAHHRR010000257         1036 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.263, whole genome shotgun sequence.
ACCESSION   DAHHRR010000257 DAHHRR010000000
VERSION     DAHHRR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.263"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..150
                     /locus_tag="NBI42_002732"
     CDS             <1..150
                     /locus_tag="NBI42_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779067.1"
                     /translation="KIEDDFIKEIKDKDSEFYSPTMANMNEYELRAMLRMMPSLIDTG
                     DDNDD"
     gene            143..529
                     /locus_tag="NBI42_002733"
     CDS             143..529
                     /locus_tag="NBI42_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779068.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGIFALLLVITLPIYTVASYQHK
                     ELHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            526..678
                     /locus_tag="NBI42_002734"
     CDS             526..678
                     /locus_tag="NBI42_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1779069.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYEKI
                     RAEVSW"
     gene            746..946
                     /locus_tag="NBI42_002735"
     CDS             746..946
                     /locus_tag="NBI42_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCD1779070.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
BASE COUNT          393 a          114 c          195 g          334 t
ORIGIN      
        1 aagattgaag atgattttat taaagaaatt aaagataaag acagtgaatt ttacagtcct
       61 acgatggcta atatgaatga atatgaatta agggctatgt taagaatgat gcctagttta
      121 attgatactg gagatgacaa tgatgattaa aaaacttaaa aatatggatg ggttcgacat
      181 ctttattgtt ggaatactgt cattattcgg tatattcgca ttgctacttg ttatcacatt
      241 gcctatctat acagtggcta gttaccaaca caaagaatta catcaaggaa ctattacaga
      301 taaatataac aagagacaag ataaagaaga caagttctat attgtattag acaacaaaca
      361 agtcattgaa aattccgact tattattcaa aaagaaattt gatagcgcag atatacaagc
      421 taggttaaaa gtaggcgata aggtagaagt taaaacaatc ggttatagaa tacacttttt
      481 aaatttatat ccggtcttat acgaagtaaa gaaggtagat aaacaatgat taaacaaata
      541 ctaagactat tattcttact agcaatgtat gagttaggta agtatgtaac tgagcaagtg
      601 tatattatga tgacggctaa tgatgatgta gaggcgccga gtgattacga aaaaatcaga
      661 gctgaagttt catggtaata gctattatca tttttgaatt aattatatta atgtgtttag
      721 caatagcact ggaggtgttg taaatatgtg gattgtcatt tcaatcgttt tagctatatt
      781 tttattgatc ttgttaagta gcatttctca taagatgaaa accatagaag cattggagta
      841 tatgaattct tatcttttca agcagttagt aaaaaataat ggtgttgaag gtttagaaga
      901 ttatgaaaat gaagttgaac gaattagaaa aagattcaaa agctaaagag ggggctaaag
      961 ccctctccag gattaaaaga agcggatatt ttttatttcg tttttgtaaa ttaaaactaa
     1021 tttatgtgaa attgtc
//
LOCUS       DAHHRR010000258         1034 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.264, whole genome shotgun sequence.
ACCESSION   DAHHRR010000258 DAHHRR010000000
VERSION     DAHHRR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.264"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(100..886)
                     /locus_tag="NBI42_002736"
                     /pseudo
     CDS             complement(100..886)
                     /locus_tag="NBI42_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282612.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          298 a          163 c          131 g          442 t
ORIGIN      
        1 aaaaaaatct tctatagaaa tgtaaccatc taaatgttga attgacataa tgtattactc
       61 ctatttgtac tttcgtttta tatttctttt attataaagt tactttagaa tattatcaac
      121 tatcgcttca ttatttctta tagttataga ttgttttata tctttcataa ttttataaac
      181 ttgagactga ttatataatg aagttttact tatatgtttt actacactat agttagtaac
      241 aatagtcgtt gtttggatag atttatttcc aataaacatt tttaagtcat tcaatgaaaa
      301 tcctgactgt ccaggatggt tatgtaacat aacaattgaa ttcggtttac tgttaaataa
      361 caattctgtt gctttttccc ctggtaaaaa ggatacacta tcttgatcac catatacttt
      421 tgtaacttta ccatctttta ataaataagc tacttcattg ctatcattgt tttctttggc
      481 atctttaagc aagtatgttg ttcttgtata aattgatttt cttctttggt atgtgttgga
      541 atatcaacat atctgacttt atcaattgct tgatcagtaa tatatatttt ctttcctaat
      601 actttagctt gttgtaattc atctttggtt gtgtcatctt ctacttgata cttaccttca
      661 cgctctttaa agaacttgtc tcgccaattg ccgacatatg gcactgtggt acttctgcac
      721 caaggatgca taggtggcac attcacacct ggtatcatat ctttaacttt aaatactttg
      781 ccgttgagtg aatgacataa tttagatgtt ttctatctat tttggcaaca tatttatatt
      841 cgccatcttc accaagttct tttaaatatg ttaacttttg tgattctgct atttcagtaa
      901 atagttaaaa aagcatataa aaatagcacc actttctctt tatctgtgta gaaaggatgc
      961 tattgatatt tagactttga ttgattaatt tctttttcta attcttcttt cttcgataat
     1021 tcttccatat gctt
//
LOCUS       DAHHRR010000259         1033 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.265, whole genome shotgun sequence.
ACCESSION   DAHHRR010000259 DAHHRR010000000
VERSION     DAHHRR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.265"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            61..585
                     /locus_tag="NBI42_002737"
     CDS             61..585
                     /locus_tag="NBI42_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCD1779071.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFSKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRILRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      572..669
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            744..>1033
                     /locus_tag="NBI42_002738"
     CDS             744..>1033
                     /locus_tag="NBI42_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCD1779072.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGG"
BASE COUNT          377 a          120 c          199 g          337 t
ORIGIN      
        1 ataatattga aactgactat aactataaga ttgttttgtt attgaaagaa gggaaaacgc
       61 atgaatcatg ataaatcacc aataatttta attggtttca tgggtaccgg taaatctacg
      121 attggtaaat acgttgcaga tgagcaaaat ttatcattta ttgatataga ttcatatatc
      181 gaagagaagt ataagttaac aataccagaa atatttagta aacatggtga acaatatttc
      241 aggaatttag agttcacatg tttgcaagaa tgtattaaca ctgcagatat aattgctact
      301 ggtggtggta ttattgaaag tgaagaggca tttaattttt tgaaaaatca aaaaaacatt
      361 atttggttag attgtaatat tgatattata tatagtcgaa tcaatgatga cccacatcga
      421 cctaatgcaa ataataagac aatcaagcag ttaaatgact tgtattgctc gcggatttta
      481 aggtataatg aaatcgcatt caagaaattt gatagtcatt tgctatcaat ttcagaaata
      541 tattatgaat tgctaaattt aataaaagcg agtgatcagt attagagaga atagagcgtt
      601 aagactctat cgccgaaggt gcaagtaatt tattacgaaa ctctcaggca aaaggataat
      661 actgtaacgc gttcctgaat tggtgattta taaacagggt agcgattgct atcctgtttt
      721 tataatttta agggggtaat tcaatgtcaa gtgatttaaa acaaacacct ttatatcaaa
      781 attatgttga tagaggtgca aaaattgtgg aattcggagg atgggcgatg cctgttcaat
      841 tttcaagtat taaagaggag cataatgctg ttcgatacga aattggcctg tttgatgtta
      901 gtcatatggg tgaaattgaa gtaacaggta aagatgctag tcagtttgtg caatatttat
      961 tatcaaatga tactgataat ttaactactt caaaagcatt atatactgct ttatgtaatg
     1021 aagaaggcgg tat
//
LOCUS       DAHHRR010000260         1027 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.266, whole genome shotgun sequence.
ACCESSION   DAHHRR010000260 DAHHRR010000000
VERSION     DAHHRR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.266"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>1027
                     /gene="ltrA"
                     /locus_tag="NBI42_002739"
     CDS             <1..>1027
                     /gene="ltrA"
                     /locus_tag="NBI42_002739"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCD1779073.1"
                     /translation="QKLLEGTYKPQAVKKVEIPKANGKKRVLGIPVVRDRVIQQAIKQ
                     VIEPSIDRTFSKHSHGFRPNRSTGTALKECASYYEAGYTIAVDCDLKQCFDNINHDKL
                     MYLFERHIKDKAVSTFIRRSLQVGAIDLSGEVAERKIGAPQGGVISTLLCNIYLHELD
                     KELEKRNHRFVRYADDFVIFVKTKRAGERVMDSIKTFIHKTLKLEVNNDKSKVGSPTR
                     LKFLSCLMSKVNGTYRFRPTTEAKRNLKRNLKWLTRRSRPGSFQDIITEINRVTRGWI
                     NYFGKGFIKRFLQELEPWLNRRIRQLILKRWKKTKTKYKMLRKYGLDHDSAMRIASSR
                     KKYWRLSS"
BASE COUNT          383 a          181 c          202 g          261 t
ORIGIN      
        1 aacaaaaact gcttgaaggt acatacaaac ctcaagcagt taagaaagtt gaaattccca
       61 aagcaaatgg gaaaaagcgt gtgcttggta tacctgtcgt aagagacaga gtgatacaac
      121 aagcaattaa acaagtgatt gaacctagta tcgatcgtac cttctctaaa cacagtcatg
      181 gctttagacc taatcgtagt acaggaacag cactaaaaga atgtgcatct tactatgaag
      241 caggttacac catagccgta gattgtgatt taaaacaatg ctttgacaac ataaatcatg
      301 ataaactcat gtatttattt gagcgccata tcaaagataa agctgtatca acatttatcc
      361 gtagaagttt acaagttggt gccattgacc tatctggcga agtcgcagaa agaaagatag
      421 gtgcaccgca aggtggtgtg atatcaacgt tactctgtaa catatatcta catgaactgg
      481 ataaagagct cgaaaaacgt aatcatcgtt ttgtgagata tgcagatgac ttcgtcatct
      541 ttgtcaaaac gaaacgtgca ggtgaacgtg tgatggatag tattaaaaca ttcatacaca
      601 aaaccctcaa acttgaagtc aataatgata aaagtaaagt gggttctcct acccgtttaa
      661 agttcttgag ttgtctaatg agcaaagtaa atggcactta tcgtttcaga cccactacgg
      721 aagcaaaaag aaatttaaaa cgcaacttaa agtggttaac cagacgaagt agaccaggtt
      781 cattccaaga tattataaca gaaattaatc gtgtaacacg aggatggatt aactattttg
      841 gaaaaggatt cataaaaaga tttcttcaag aattagaacc ttggctaaat cgccgcatta
      901 ggcaactcat actcaaaaga tggaagaaga ctaaaacaaa atataagatg ttgcgcaagt
      961 atggtctaga ccacgatagc gcaatgagaa tcgcgagctc aagaaagaaa tactggcgtt
     1021 tatcatc
//
LOCUS       DAHHRR010000261         1023 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.267, whole genome shotgun sequence.
ACCESSION   DAHHRR010000261 DAHHRR010000000
VERSION     DAHHRR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.267"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..249
                     /locus_tag="NBI42_002740"
     CDS             <1..249
                     /locus_tag="NBI42_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCD1779074.1"
                     /translation="VPSYDVKYKMSNKDEIVKELRSRYNIPTDKAPVLKMHIDGNLKG
                     SSVGYKKLEIDFSKGEKSDLSVIDSLNFQPAKVDEDDE"
     gene            268..>1023
                     /locus_tag="NBI42_002741"
     CDS             268..>1023
                     /locus_tag="NBI42_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1779075.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCGGNKITGDSKEDQIKKSFAKT
                     LDMYPTKNLEDFYDKEGYRDGEFKKDDKGTWLIRSEMKIQLKGENLESRGAVLEINRN
                     TRTAKGNYIVREVVEDSDGMTHNHTKRYPVKMEKNKIIPLKPIDDEKIKKEIKKFKFF
                     VQYGNFKELENYKDGEVTYNPEVPIYSAQYQLKNSDYNVEQLRKRYNMPTKKAPKLLL
                     KGSGNLKGSSVGYKNIEFTFVENKEENIYFTDSV"
BASE COUNT          431 a          112 c          204 g          276 t
ORIGIN      
        1 gttccaagtt atgacgtgaa atataaaatg agtaataaag atgaaatagt taaggaatta
       61 agaagtcgtt ataatattcc tactgataaa gcaccggtat taaaaatgca tattgatggt
      121 aatttaaaag gaagttctgt gggttataaa aagttggaaa ttgacttttc aaaaggtgaa
      181 aaaagcgatt tgtcagtaat agattcttta aatttccagc cggcgaaggt ggatgaagat
      241 gatgaatgat gaggatggtg tgtaacaatg aagtctataa aaaggattgg attgtgcata
      301 agtctgttga ttttaattat ttttgtaaca tcttgtggtg gtaataagat cactggagac
      361 tcaaaagaag accaaatcaa aaagagcttt gcgaaaactt tagatatgta tcctacgaaa
      421 aatctagaag acttttatga taaagaagga tatcgagatg gagaatttaa aaaagatgat
      481 aaagggactt ggctaattag atctgaaatg aaaatccaat taaaaggaga aaatctggaa
      541 tctaggggag cagttttaga aattaacaga aatactagaa cggctaaagg gaattatatc
      601 gttagagaag ttgttgaaga tagcgatgga atgacacaca atcatacaaa aaggtatcct
      661 gtaaaaatgg aaaagaataa aatcattcca ttaaaaccaa tcgatgatga aaaaattaaa
      721 aaagaaatta aaaaatttaa attctttgta caatacggga atttcaaaga attggaaaac
      781 tataaagacg gagaagtgac atataaccca gaagtaccaa tatactctgc acaatatcaa
      841 ttgaaaaaca gtgattacaa tgttgaacaa ttaaggaagc gatataatat gccgacgaaa
      901 aaagcgccta aattattatt gaaagggtca ggtaatctaa aaggctcatc agtcggatat
      961 aaaaatattg aatttacctt tgttgaaaac aaggaagaaa acatttactt cacagatagt
     1021 gtc
//
LOCUS       DAHHRR010000262         1017 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.268, whole genome shotgun sequence.
ACCESSION   DAHHRR010000262 DAHHRR010000000
VERSION     DAHHRR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.268"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            14..730
                     /gene="splE"
                     /locus_tag="NBI42_002742"
     CDS             14..730
                     /gene="splE"
                     /locus_tag="NBI42_002742"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HCD1779076.1"
                     /translation="MNKNIIIKSIAALTILTSMTGVGTTMVEGIQQTAKAEHNVKQIT
                     NTNVAPYSGIVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTSILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNTIISDAFVEPGNSGSAVFNSKYEVVGVHFAGNGPANKSTK
                     GYGVYFSPEIKKFIADNTDK"
BASE COUNT          397 a          158 c          162 g          300 t
ORIGIN      
        1 gaggagcaga aaaatgaata aaaatataat catcaaaagt atagcagcat taacaatttt
       61 aacatcaatg actggtgtcg gcacaacaat ggttgaaggg attcaacaaa cggctaaagc
      121 cgaacataat gtgaaacaaa ttacaaatac aaatgttgca ccatacagtg gtatcgtttc
      181 gataggatct ggaacaggtt tcattgtcgg taaaaataca attgttacta acaagcatgt
      241 cgttgcaggt atggaaattg gcgcacatat cattgcgcat ccgaatggtg aatataataa
      301 tggcggattt tataaagtta aaaaaattgt tcgttattca ggtcaagaag atattgccat
      361 tctacatgtg gaagataaag ctgttcatcc aaaaaatagg aattttaaag attacacaag
      421 tatattaaaa atagcatcag aagcgaaaga aaatgaacgc atttcaattg ttggctatcc
      481 agaaccttat ataaataaat ttcaaatgta tgaatcaaca ggaaaagtgc tatcagtgaa
      541 aggtaatacg attatttctg atgctttcgt agaaccaggc aactcaggtt cagcagtatt
      601 taatagtaaa tatgaagttg taggtgttca ctttgctgga aacggccccg caaataaaag
      661 tacaaaagga tatggtgttt atttctctcc tgaaattaag aaattcatcg cagataatac
      721 agataaataa ttaaaactta ggcattcacc taatcctgac aaaatatact ataactaaca
      781 tttattaata tatattgcat tacttattat gcattaaagc caatcaatga ttgatttaca
      841 ccatttcaat tgttgattgg ttttacttat gtgtaaatgt acaacttatt gacatcattg
      901 aaaatataga taattttgaa aagctacaac attaccataa aatttttcaa ttataatcgt
      961 gccaccaaaa tataaaaatt tccaccacca acatcaaaat tctccacatc gcaacat
//
LOCUS       DAHHRR010000263          996 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.269, whole genome shotgun sequence.
ACCESSION   DAHHRR010000263 DAHHRR010000000
VERSION     DAHHRR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.269"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..380
                     /locus_tag="NBI42_002743"
     CDS             <1..380
                     /locus_tag="NBI42_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1779077.1"
                     /translation="LTVNENLDIGLAYKKISKKEKEQLKIRYIEQFGLSNSLKRKVHT
                     LSGGEQQRVALIRMMLKDPTVMLADEPTGALDPKTGQMIIQSLFGLVNENKVLILATH
                     DMAIANQCDEIIDLEQYRKVASM"
     gene            complement(468..758)
                     /locus_tag="NBI42_002744"
     CDS             complement(468..758)
                     /locus_tag="NBI42_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779078.1"
                     /translation="MFYACHFKIKIGGLILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT          413 a          121 c          151 g          311 t
ORIGIN      
        1 atctgacagt aaatgaaaat ttagatattg gattagcata taaaaaaata agtaagaaag
       61 aaaaagaaca attaaagata cgttatatag aacagtttgg tctgtcaaac agtttaaaaa
      121 gaaaagttca tacactaagt ggtggagaac aacaacgtgt cgctttaatt agaatgatgt
      181 taaaagatcc tactgttatg ttagctgatg aaccaacggg tgcgttagat cctaaaacag
      241 gacagatgat tattcaatca ttatttggtt tggtcaatga aaataaagtg ctaattttag
      301 caacacatga tatggctatt gcaaatcaat gtgacgaaat aatagattta gaacagtata
      361 gaaaagtagc atctatgtga tatccaaaag ataaaattaa aagccaatca attgggaatg
      421 ttaatttcaa cactccgttt gattggcttt tataatgcgt tgctatgtta tgaaagtaat
      481 aacggcaaac tgaaaaaaag caatataata atatttaata cgattagtaa taaatgtaat
      541 tgatttttcg ttttaatata gcaaaaaaca atgactaaag ataacaacaa tatcatgatt
      601 gcacctggga agaaccataa tgctgaagct gtgtaccaaa tcattggtaa cgccataata
      661 ataaaagata taagacaaga aaggatacca ataatattat aagttctagt caaaattaaa
      721 cctccaattt ttattttaaa atgacatgcg taaaacaatc attttaaata catcatacaa
      781 taaaattaaa ttgtatgata gatgaatttg aaattaaatt gaattggctt atctttcgta
      841 aacataatta tgacgtaaca atccttataa gtggaacgag tgtaattata ccatcagtcg
      901 tattaatcat aaaatttaat ctatgttgtt attgattttt atattacaaa taccgttcta
      961 acattgtgcg aaatattatt ttactcaaat aaacga
//
LOCUS       DAHHRR010000264          996 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.270, whole genome shotgun sequence.
ACCESSION   DAHHRR010000264 DAHHRR010000000
VERSION     DAHHRR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.270"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            91..297
                     /locus_tag="NBI42_002745"
     CDS             91..297
                     /locus_tag="NBI42_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779079.1"
                     /translation="MNEIETIISEIEKLLANNTPYSISKNSGVPRQTVTDLNVGKTKI
                     KEAKFKTIIKLYEYQRTLENKTEC"
BASE COUNT          394 a          100 c          139 g          363 t
ORIGIN      
        1 ttgtcaatta caaagaaaca atgtattgat aaacgtagct attgcattag tagtactaaa
       61 attattaaaa gattgtgagt gtgattaaaa atgaatgaga ttgaaacaat tataagtgaa
      121 atagaaaagt tattagctaa caatacacca tatagtattt caaaaaattc aggtgtacca
      181 cgtcaaacag ttacagattt aaacgtaggt aaaactaaaa taaaagaagc taaatttaaa
      241 acgataatca aattatatga atatcaaaga acattagaaa ataaaacaga atgttaacaa
      301 aaatattttg ttaggttagg tggacaagaa attaaatttc taatgatttc atggttgtct
      361 gtccacgttt tgtccacctc aataagataa tctcttagtt ttagaaattg tacatggtta
      421 aacaatcaaa aatgcatatt atgaaattat cattgccatt aaatcttgaa taatctttaa
      481 gtgatgatat taaaggttta acacaataaa taaattagct ttaatgttgt attctatact
      541 gtatatgttt agtaataaca tatgtccttt tttattgttc gctttttatt atcactatat
      601 tagatgttgc atttgacata tgtagcaatc tatgctaatt atattttaat gtattttaat
      661 tttatgatta taaaattaaa aatagttgaa atcgcacttt atttcgtgaa agtattttta
      721 atacatttat ttgaaaatat tttatcataa atatacatag gtaaatgttt cttaacaaac
      781 ttatttgttc gagtaaaaaa ttcgcgtggt aaacataatt tacataaatt ataaggaggt
      841 gaaaatggtc gaggaagtta aaacggtaaa atagttagaa tgttttgatt tcaattctgg
      901 taggactatt gaatttgcaa actaataaga atgtgaggtt tgatttaata cgaattcata
      961 ttctttgaat aaaatattac tttctcacca ttagta
//
LOCUS       DAHHRR010000265          995 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.271, whole genome shotgun sequence.
ACCESSION   DAHHRR010000265 DAHHRR010000000
VERSION     DAHHRR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.271"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(730..954)
                     /locus_tag="NBI42_002746"
     CDS             complement(730..954)
                     /locus_tag="NBI42_002746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779080.1"
                     /translation="MRRILETIIKFNGYDQNKFYENDLVALKLFNVNSHSIDDLTADM
                     IGNDVEELLRIFREMFKNNNIEDHFNKRWN"
BASE COUNT          282 a          195 c          141 g          377 t
ORIGIN      
        1 tccataagtt gccaactagt tcgttgtttt ctgcatgatg taaatctata acctccattc
       61 tttactttgt atgaatattg ttcagtcctt cggtacattt tccctactat gacgtctgct
      121 gacttctcat tattcgttgt tactacggta tgtgtaccgc taatgagacc tccctgggta
      181 aggtgcaacc actttccact catctcatcg catcatttac catataaaat tcgggtagta
      241 ttggacttcg tcttgtttag cagactcatc cgttttatat ggccttatta tgatgtttct
      301 gttcgtcaat gcgagtgttt gcgtccgact tccttcagat tccacttcac aatggacacc
      361 cttgtctttc gctaacagtt cctactacca agcctgtaac ggactttcac cgtcaagttg
      421 ttgcccatgc caggcgcacc aaaaaaacac ctcgacataa cttcgaggtg ttttgtatat
      481 atcgtccgac aattaacgtt ttgagaattg aggtgaacga cgagctactt taagacctgg
      541 tttactttag ggtttcgtat agttgcacat actgtcttaa ggcatgatat tataggattt
      601 ctgatttttt cgtttacata gaattgcatc aaatatcact taagagtaac taaataaatt
      661 gctggctggt aataatttta atatctataa tttttaaaat tatacttaat attttttaat
      721 tagtaatacc taattccatc ttttattgaa gtgatcctca atattattat ttttaaacat
      781 ttctctaaaa attcttaata actcctcaac atcatttcca atcatatcag ctgtcaaatc
      841 atctattgaa tgagaattaa cattgaatag tttaagtgct actagatcat tttcataaaa
      901 tttattctga tcatacccat taaacttaat aatcgtttct agtattcttc tcattgaatt
      961 tagcatagaa ttaataagtt ttaattcata cattt
//
LOCUS       DAHHRR010000266          966 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.272, whole genome shotgun sequence.
ACCESSION   DAHHRR010000266 DAHHRR010000000
VERSION     DAHHRR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.272"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            28..>966
                     /locus_tag="NBI42_002747"
     CDS             28..>966
                     /locus_tag="NBI42_002747"
                     /inference="COORDINATES: protein motif:HMM:NF037833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779081.1"
                     /translation="MSGEEIVSEVYKEIFKEISLNIDKNSLPPVITNRFFAINSLSDA
                     ESINNQQGNSSKLVTLESYKIYGPHQTGTLNASDPNTKENQAIKLVPGPGTGNKEQNR
                     TAIYAPNTIDFRKDFNMNFDIALWPQGMSSRPDGLGFMFSKSTPEEYLSQGGILNDNG
                     VSNAFGFKIDTYNNGNVDNLEKDFSKEKGASPKAYGTFVYNDANGTTHSYTKYPLDNQ
                     NRQQKNNLFNGFTQAFNGFRGQQYNTVNINYNADSGKLTVNYAGLTWTALLSDIGADK
                     NSRYNFGIISSKETDRNVPSSHHTVKVNSASITYTPG"
BASE COUNT          367 a          135 c          167 g          297 t
ORIGIN      
        1 gatgataaaa atattacatt tgacagaatg agtggagaag agattgtttc cgaagtttac
       61 aaggaaatat ttaaagaaat ttcattgaat attgataaaa attctttacc accagtaatt
      121 actaatagat tttttgctat taatagttta agtgatgctg agtctataaa taatcaacaa
      181 ggaaacagct caaaattagt tacgctagaa tcttataaaa tatacgggcc tcatcaaact
      241 ggtacattaa atgcatctga tcctaataca aaagaaaatc aagctattaa gttggttcct
      301 gggccaggta ctgggaataa ggaacagaat agaacagcta tatatgctcc aaatactata
      361 gattttagaa aagactttaa tatgaatttt gatattgcgc tatggcctca aggcatgtct
      421 tcaagacctg atggattagg attcatgttt tcaaaatcta ctcctgaaga atatcttagt
      481 caaggtggca ttctaaatga taacggtgtg tcaaatgctt ttggttttaa aatagatacc
      541 tataataatg gtaatgtaga taatttagaa aaagattttt ctaaagaaaa aggtgcttcg
      601 ccgaaagctt atggaacgtt tgtttataac gatgctaatg gaacaactca tagttatact
      661 aagtacccgt tagataatca aaataggcaa caaaaaaaca atttatttaa tggtttcact
      721 caagctttta atggctttag aggccaacaa tataatactg taaatattaa ttataatgct
      781 gatagcggta agttgaccgt gaactatgct ggactaacat ggacagcact attatcagat
      841 attggagcag acaaaaatag tagatataat ttcggaatta tatcaagtaa agaaacagat
      901 agaaatgttc caagtagtca tcatacagtt aaagttaatt ctgcctcaat aacttatacc
      961 ccaggt
//
LOCUS       DAHHRR010000267          956 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.273, whole genome shotgun sequence.
ACCESSION   DAHHRR010000267 DAHHRR010000000
VERSION     DAHHRR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.273"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            333..>956
                     /locus_tag="NBI42_002748"
     CDS             333..>956
                     /locus_tag="NBI42_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HCD1779082.1"
                     /translation="MKKNIMNKLVLSTALLLLGTTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHLSLSGKWLWQKPNGTIHATLQTWVWYTHIQVFGPESWGNINQL
                     RDKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKHYKLSLKYRDKLQTMIGGL
                     EFYQGNKPVITLKELDFRVRQTLIKNKKLYNGEFNKGQIKITGGGNNY"
BASE COUNT          364 a          136 c          144 g          312 t
ORIGIN      
        1 cattttatta atattaaatt taagtagtag gaaattatta aaataagtac tacatttaaa
       61 gtataactat ttttcaagta gttagaaaat tcaattatca aacaatttaa tgcaattgat
      121 tatggaataa ttctaacgtg tcgtttttaa tataaaactc ccgcctactt tattaggtac
      181 tgtttctgtc caaaacttaa aaatgataag ttttgcttaa ataacactac taactgttta
      241 agtttattta acatagtttt agcttttatt taattccgaa tcggtgtgat agcttatata
      301 ctttgggtaa ttcacgcaaa ggagattttc atatgaaaaa gaacatcatg aataaattag
      361 ttttatcaac agcattgtta cttttaggaa ccacatcaac acaacttcct aaaacaccaa
      421 tcagtttttc atctgaagca aaagcctata atattagtga aaacgagact aatatcaatg
      481 agttaataaa gtattataca cagcctcatt tatcactatc cggaaaatgg ttatggcaaa
      541 aacctaatgg taccattcat gcaacattac aaacgtgggt ttggtatacg catattcaag
      601 tattcggccc tgaaagttgg ggaaacatta atcaattaag agataaatat gtagatatat
      661 ttggaactaa agacgaggat acagtggaag gttactggac ttatgatgaa acatttactg
      721 gtggtgttac accagccgcc acttcatctg ataagcatta taaattatct ttaaaatata
      781 gagataaact acaaactatg attggtggac ttgagtttta ccaaggtaac aaaccagtaa
      841 taactttaaa agaactggat ttccgcgttc gtcaaacttt aattaagaac aaaaaactat
      901 ataacggaga atttaataaa ggtcaaatta aaattacagg tggtggtaat aattat
//
LOCUS       DAHHRR010000268          937 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.274, whole genome shotgun sequence.
ACCESSION   DAHHRR010000268 DAHHRR010000000
VERSION     DAHHRR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.274"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(182..733)
                     /locus_tag="NBI42_002749"
     CDS             complement(182..733)
                     /locus_tag="NBI42_002749"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCD1779083.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
BASE COUNT          305 a          138 c          160 g          334 t
ORIGIN      
        1 taaattaatg ttaagaaaga attaaaggat tttatatgaa atggtaaaat ttaaatgagg
       61 ggataaaaag cttggtagaa gggtgaaaat attttactgt tatttattag gaaaagttgt
      121 aaaagaaaaa gcttaggaca tcatatttat cccaagcttt gataattagt aaaaatgtgt
      181 tttaaacttc tacagcatct gtatgtggtt gtaatgggtg atctttgtca atgtgatcat
      241 agaacattac accatttaaa tggtcaattt catgttggaa aacaattgct ggatatcctt
      301 ttagacgtaa ttgtatatca ttaccttcga tgtctttggc tttaattgta attctattat
      361 gacggtgaac tagaccagca acattatcat cgacactaag gcaaccttca ccagttggta
      421 aataagcttc ttgaacgcta tgacttacaa ttttggggtt cacaagcata tagtcataag
      481 atttgccact gccatcatct ggtattaaaa cagcaatcat acgtttagaa atattaattt
      541 gaggtgcagc caaaccaacg cctgaacgta aaccgtagcg tttcgcgatt tcctcatctt
      601 gactatttac taaaaactct ctcatggcga ttaatgtttc tttttcttct ttagttaatg
      661 gtaattctag ctcggctgct ttttgacgca aagttggatg accatctcta atgatgtctt
      721 tcattgttaa catatattgc accttcctta ttttaatttg ttttagttga atgacagtaa
      781 aaaggttgtt aagatactca tacattttta tgtgtaagta tgtacaaagt taaccaacta
      841 ctgccaaggt ttattttaga tagtatatgt aaattttcag atatgctaat tgcctaaaaa
      901 atgattaaag tgttgatttc aagcaacgat actttag
//
LOCUS       DAHHRR010000269          926 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.275, whole genome shotgun sequence.
ACCESSION   DAHHRR010000269 DAHHRR010000000
VERSION     DAHHRR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.275"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(126..>926)
                     /locus_tag="NBI42_002750"
     CDS             complement(126..>926)
                     /locus_tag="NBI42_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCD1779084.1"
                     /translation="NNLNDLLNGNLESLNKKESIKETAKVVRAKILQDESNEALLFML
                     EKAGIVIYEKRINDSIDAYSFWSKDQTPFIILGTNKGVAVRRNFDLAHELGHLVLHRH
                     IQFDLLSPEEYKTIEHEADIFASEFLLPEEAFKKDFDQMTKKSNPDYLAVLKEKWYVS
                     IQAIAMRAYYLGLMSSTQYRYFWASLNKKGYKSKEPLDDVIEMSRPVKMNSLLKLYFD
                     RNILTPQKLLNYLKVDETFLNHLAGINLKLFKDYVNENREYNITNLYK"
BASE COUNT          294 a          162 c          120 g          350 t
ORIGIN      
        1 ttgttatgtc gcacaaaaca caacattaaa aggtccttgt cgatacctat caacagatgt
       61 tacaaataaa aaaaactcga actgaaatga tgttcttttc agctcgaggt ttaaatattg
      121 gtaccctatt tatatagatt cgttatatta tattctctat tttcattaac ataatcctta
      181 aagagtttta aattaatacc tgctagatga ttcaaaaatg tttcatcaac ttttaaataa
      241 ttcaataatt tttgtggtgt cagtatattt ctatcaaaat acaactttaa taaactattc
      301 attttgacag gacgtgacat ttcaatcacg tcgtctaaag gctctttact tttatagcct
      361 tttttattta atgatgccca aaaatatctg tactgagtac tagacattag acctagataa
      421 taggctctca ttgcaattgc ttgtatagaa acataccatt tttctttcaa tacagctaaa
      481 tagtcaggat ttgatttttt tgtcatttga tcaaagtctt ttttaaacgc ttcttcaggt
      541 agtaaaaatt cagaggcgaa aatatctgcc tcatgttcaa ttgtcttata ttcttcagga
      601 gataataagt cgaattgaat atgacgatgt agaacgagat gtcccagctc atgtgcaaga
      661 tcaaaattcc ttctaactgc taccccctta tttgtaccca gtataataaa aggtgtctga
      721 tctttactcc aaaaactata tgcgtctatg ctatcattaa ttcgcttttc gtatataact
      781 atacccgctt tttcaagcat aaataatagt gcttcattgc tttcgtcttg aagaatttta
      841 gccctcacaa ctttagcagt ttcttttatg ctttcttttt tatttagtga ttctaaattg
      901 ccattcaaca agtcatttaa attatt
//
LOCUS       DAHHRR010000270          893 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.276, whole genome shotgun sequence.
ACCESSION   DAHHRR010000270 DAHHRR010000000
VERSION     DAHHRR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.276"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
BASE COUNT          373 a          127 c          104 g          289 t
ORIGIN      
        1 gttaatatta catttagtaa catcaaattt aacaaatata gaagaaaatc ctcaactttc
       61 taattatgaa tcagaatatt atcttcttga aaaaaattta attaagacac aatttatcaa
      121 aagtactaaa agtagaagtc tatatgaaga aaccacttat cacaaattga cgaatggaga
      181 catcttaaac caacccggaa ctttgaaatt atggacgtag gcaaggcttt agtccaaccg
      241 tttcattgca tgaatttgta aaaacatgtc agatacctat ttgaaattac cgaatattta
      301 catttaagtg aatcataaat tttagaagta ttatgataat atataccatt aaaaccaatt
      361 actaattcat tgttctaatt atgcaggtaa ctttccgcaa taagtccaaa ataaatctaa
      421 tggataaaaa ataatcaaaa ctttaacttt aatcaatatt gaactgcaaa aatttggcac
      481 aagctaaaac aatgagtaaa ccgacaggat tgccaatgac acagataaat cagtagaaga
      541 aatgatgtgt agaagttcac aagtaaactg gaaaataggg attaattaaa attcgtttca
      601 acctttatac atactaagca aaattacaca ctttataaaa ttatattaat tttgtaaaat
      661 tcagcttatt tggaggatta atagcaatga taaaacaggt tctaagaaaa agtttaaata
      721 cagtaaaaga acatatcgat aaaagtaact tccaaaaaaa cttaatattt tcacttgtca
      781 tattcccatt cttatttgtg ccttacatta taatagatat actcgtacat ttatctaata
      841 atcaagaata ctttaagtta ttatcatcta tttcacatat atttttaatg ttc
//
LOCUS       DAHHRR010000271          879 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.277, whole genome shotgun sequence.
ACCESSION   DAHHRR010000271 DAHHRR010000000
VERSION     DAHHRR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.277"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..787
                     /locus_tag="NBI42_002751"
     CDS             <1..787
                     /locus_tag="NBI42_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HCD1779085.1"
                     /translation="DNTIKSISKDYFDYLQTESKFIPLENETIEFYSPIVDYFGDSIS
                     VNISFSGNRYKLADHGETLWNMEQFGLDLTNHKKQKKYQLLNNIVQNYGLVIENKHIN
                     FYTNRIDLSQAIHDYVLAISEISYLGILKKENIKSLFKDEVMQYFLSNRKIYPNMFLE
                     FKVEGKSKLIHSFEAVFPGEATEYVKTIKRIDKNNAKNVLFDWNDVEVHRNKNFDSNA
                     RLNIITEQQDDISEAVSTMLSQYNVEVFSFENKKQLEEKFSNV"
BASE COUNT          350 a          114 c          124 g          291 t
ORIGIN      
        1 tgacaacaca attaaatcta ttagcaaaga ctattttgat tacttacaaa cagaatcaaa
       61 atttatccca ttagaaaatg agactataga attttattca cctatcgtcg attattttgg
      121 tgattctatt tctgtaaata tatctttttc agggaatcga tataaactag ctgatcatgg
      181 tgaaacttta tggaacatgg aacaatttgg tttagattta actaatcata aaaaacaaaa
      241 aaagtatcaa cttctcaata atattgtaca aaattacggt ctagtcattg aaaataagca
      301 cataaacttt tatacaaata gaatagactt atctcaagcc attcatgatt atgttttggc
      361 tatttccgaa attagttatt tgggaatatt aaagaaagaa aatattaagt cattatttaa
      421 agacgaagtg atgcaatatt ttctatcaaa cagaaagata tatcctaata tgtttctcga
      481 atttaaggta gagggaaaat ctaagttaat ccatagtttc gaagcagtat ttccaggaga
      541 agctacagag tatgttaaaa caattaaacg aatcgataaa aacaatgcaa aaaatgtcct
      601 tttcgattgg aatgatgttg aagtacatag aaataaaaat tttgattcta atgctagact
      661 taatatcatt acagaacaac aagatgatat tagcgaagca gtatcaacta tgttatctca
      721 atataatgta gaggtgtttt catttgaaaa caaaaaacaa cttgaagaaa agtttagtaa
      781 cgtataatta tcgtgttata tagtgtttct tcgataatag tttctatttc aactaaatca
      841 ccgatactta aaatgataaa tttaccaata ccaagtttg
//
LOCUS       DAHHRR010000272          844 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.278, whole genome shotgun sequence.
ACCESSION   DAHHRR010000272 DAHHRR010000000
VERSION     DAHHRR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.278"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..822
                     /locus_tag="NBI42_002752"
     CDS             <1..822
                     /locus_tag="NBI42_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCD1779086.1"
                     /translation="KNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVK
                     DITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFKIFL
                     EDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPI
                     FITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLKTKEVQKILSHRAKGIKMYIFV
                     QKKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN"
BASE COUNT          367 a           84 c          141 g          252 t
ORIGIN      
        1 aaaaatttgg ttttcttttc aaggcaaata gcacctggat tgaaaagaat cgatagttta
       61 gtcttagaag aacttttaaa aaatgaatta acatatgatg aattaaaaaa taaaatgttg
      121 aacgaagtta aggatataac agaagatgat atagatactt cattaagaat tttagatttt
      181 tcattttaca acgcaggaat tgaaaaaatc tacggaagtc ctattataga acgcaatgaa
      241 agaatgataa gactaagcga tgcatttact aatgctctaa gtaatcaaac atttaaaata
      301 tttttagaag atcttattga attatcaaag tataacaatg aaaaatacca aaaaggtaaa
      361 aatggactga ttttatataa taaatattca agagaagatt ttagcaaaat atttaattgg
      421 aataaaaatg gttcgtctgt aatcatggga tacatgataa aaagccaaga aatgccaata
      481 tttatcactt atgataagca tgaggatata agtgatagca caaaatatga agatgaattt
      541 ttaagtcaag atgaattaaa atggtttact aaatctaata gaacgctaaa aacaaaagaa
      601 gttcagaaaa ttttgtccca tagagcaaaa ggaattaaaa tgtatatttt tgtacagaaa
      661 aaagatgatg atggtatata tttttattat ttaggaactg ctggatatat agaaggttca
      721 gagaagcaag ataaaatgcc gaatggttca aacgtagtaa cgatggattt agcactcgat
      781 aaagctgtaa gagatgatat atatcgctat ctaacaaatt aatgtaaacc gaaaaatgaa
      841 tgtt
//
LOCUS       DAHHRR010000273          844 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.279, whole genome shotgun sequence.
ACCESSION   DAHHRR010000273 DAHHRR010000000
VERSION     DAHHRR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.279"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..705
                     /locus_tag="NBI42_002753"
     CDS             <1..705
                     /locus_tag="NBI42_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1779087.1"
                     /translation="SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSEAGKHTPAKPMSTVKDHHNKAKALPETGSENNNSNNGTLFGGLFA
                     ALGSLLLFGRRKKQNK"
BASE COUNT          297 a          184 c          172 g          191 t
ORIGIN      
        1 agcgattcag actcagacag cgattcagac tcagacagcg attcagattc agatagcgac
       61 tcagactcag atagcgactc agactcagat agcgattccg actcagatag cgactcagat
      121 tcagatagcg actcagactc agatagcgat tcagattcag atagcgactc agattcagac
      181 agcgattcag attcagacag cgactcagat tcagacagcg attcagattc agatagcgat
      241 tcagactcag acagcgactc agattcagac agcgactcag actcagacag cgactcagac
      301 tcagacagcg attccgactc agacagcgat tcagactcag atagcgactc agattcagac
      361 agtgactcag actcagatag tgattccgat tcagacagtg attccgactc agacagtgac
      421 tcagactcag atagcgattc cgactcagat agcgactcag actcagacag cgattcagac
      481 tcagatagcg attccgactc agacagtgac tcagactcag aagcaggtaa acatacaccg
      541 gctaaaccaa tgagtacggt taaagaccat cacaataaag caaaagcatt accagaaaca
      601 ggtagtgaaa ataataattc aaataatggc acattattcg gtggattatt cgcggcatta
      661 ggatcattat tgttattcgg tcgtcgtaaa aaacaaaaca aataatatga ttaacttaac
      721 caggtccgtg tggcctggtt tttctgttta gaaattccca gatataaaac aaaaaatctt
      781 taaggaatag atacaattta atttgttaca gaaatgtaat tgtctatgaa ttagaaaagc
      841 taag
//
LOCUS       DAHHRR010000274          832 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.280, whole genome shotgun sequence.
ACCESSION   DAHHRR010000274 DAHHRR010000000
VERSION     DAHHRR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.280"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>832
                     /locus_tag="NBI42_002754"
     CDS             <1..>832
                     /locus_tag="NBI42_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779088.1"
                     /translation="NKTIVADGGNSEGGAGAKYQGAKHPNEKVVATDSAMIPYAAWQK
                     FARPRFDNMISFNSTNDLLTWLQDPFIKDMPGKRVNINDGVPRLDALIDSHVGYKRKL
                     NRKDNTYDTVPLIKIKSVKDTEIKNGKKVKKTINITLDMDGRIPINVWTGDSIARSGK
                     GTLIKLNLENLDALSKLITGETSGMLAECVIFLNESFNISENENKNFADRKKQLSEGF
                     KDKINLFQLEEMERTLISKINSLEEVADETIESISAVKHLLPDFALDALKERINELFK
                     G"
BASE COUNT          337 a          103 c          163 g          229 t
ORIGIN      
        1 aaaacaaaac cattgtagcg gatggtggta attcggaagg cggtgcagga gcaaaatatc
       61 aaggagcgaa acatccaaat gaaaaagttg ttgctactga ctcagcaatg attccttatg
      121 ctgcttggca gaaatttgct agaccacgct ttgataatat gattagtttt aatagtacca
      181 acgatttatt aacatggtta caagatccat tcatcaaaga tatgccagga aaacgcgtta
      241 acattaatga tggtgtaccc aggttagatg ctttaataga cagccatgta ggttataaaa
      301 ggaagttaaa tagaaaagat aacacatacg atactgtacc actaatcaaa attaagtcgg
      361 taaaagatac agaaattaaa aatggaaaaa aagtaaaaaa gactattaac ataacattag
      421 atatggatgg gcgaattcca ataaatgttt ggacgggaga ttcgattgca cgttccggaa
      481 aaggaacttt aattaaactg aatttagaaa atcttgatgc gttgagtaaa ctgataaccg
      541 gtgaaactag tggtatgtta gcagaatgcg taatcttttt aaatgaaagt tttaacatct
      601 cagaaaatga aaataaaaat tttgcagata gaaagaaaca attatcagaa ggatttaagg
      661 ataagattaa cttatttcaa ttagaagaaa tggaaagaac tttaattagt aaaataaact
      721 cacttgaaga agttgcagat gaaacaatag aaagtattag tgctgttaaa cacttattac
      781 ctgattttgc attggatgca ttaaaagaaa gaattaatga gttgtttaaa gg
//
LOCUS       DAHHRR010000275          829 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.281, whole genome shotgun sequence.
ACCESSION   DAHHRR010000275 DAHHRR010000000
VERSION     DAHHRR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.281"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            179..574
                     /gene="spxA"
                     /locus_tag="NBI42_002755"
     CDS             179..574
                     /gene="spxA"
                     /locus_tag="NBI42_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCD1779089.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
BASE COUNT          302 a          107 c          120 g          300 t
ORIGIN      
        1 actttttatt aagtgtagaa acttatctga aaaattaggt ttttctcatt taattttaat
       61 gttagatagt tataataaat agtaacgatt aagaatttta gaaaaaaatg ttttttcttg
      121 atctataact aaattttaag caatgaattt tattcattca aataggagag tgagatgtat
      181 ggtaacatta tttacttcac caagttgcac atcttgccgt aaagcgaaag catggttaca
      241 agaacatgac attccgtata cggagcgtaa tattttttct gaacatttaa caattgatga
      301 aattaagcaa atattaaaaa tgactgaaga cggtactgat gaaatcattt ctacacgttc
      361 taaaacatac caaaaattaa atgttgatat tgattcacta ccattacaag acttatattc
      421 aatcattcaa gataatcctg gcttattacg tcgaccaatt attttagata ataaacgact
      481 acaagttggt tataatgagg acgagattcg acgtttctta cctagaaaag ttcgtacgtt
      541 ccaattacaa gaagcacaac gtatggttga ctaataacca tttaactatg tgtcatactt
      601 tactaaaaga tatgtcaagc aacactattt ctatagtgtt gcttttttgg ttttaatttt
      661 acaataattg cttagtatct tttaaatcaa atgtgcaatt gttaatatag ttctatatta
      721 gagtgttttg gatgaaacat ttgctatgat aaggtaaaat taaattaaga aagtgcttta
      781 atgctttaac tgtataaatc atttaaatag ttttgctaca acgaatatg
//
LOCUS       DAHHRR010000276          829 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.282, whole genome shotgun sequence.
ACCESSION   DAHHRR010000276 DAHHRR010000000
VERSION     DAHHRR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.282"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..201
                     /locus_tag="NBI42_002756"
     CDS             <1..201
                     /locus_tag="NBI42_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCD1779090.1"
                     /translation="SEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEYEEEYKK
                     EQEREKEKEKQKDDDHSGLDEV"
     gene            complement(487..>829)
                     /locus_tag="NBI42_002757"
     CDS             complement(487..>829)
                     /locus_tag="NBI42_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HCD1779091.1"
                     /translation="ISTIYSLSVIIGVTFSYKLNEGIAIFIGILPHGILEIYLTSIEL
                     SMLFLLNAYIRKNSMNLFRKRKEALPKFFVLLKSIVKCYLLIFLPVAFLCALIEITVT
                     PTVYKFLTNII"
BASE COUNT          341 a          112 c          111 g          265 t
ORIGIN      
        1 tcagaagata aaacagttga ttctaaatca ttcaaattta gtaaacttaa aaccgaagat
       61 ttttctcata gagcggaaac aagagaagaa gtagaaaaga aagaaaagga atatgaagaa
      121 gagtacaaaa aagaacaaga acgagagaaa gaaaaagaaa agcaaaaaga tgacgaccac
      181 agtggtttag atgaagtata aatttatgaa tgctcgcatt tagcgttata atctttgttg
      241 ttattattgt cattatagat tagcttactt acactcataa tgacatacta atttaattgt
      301 taaatacaac aaaaaaagag tagctcgcct actctttttt gttgttttga aatggtcatt
      361 tttgaaatga ctcgatcata atttcacgaa taccatgcaa catatttcgt gaactcattt
      421 ttcaaacaat acttatcgat caaaaataaa cctatacaca tatttagcta tgccctttta
      481 aaagttctat attatattag ttaaaaattt gtaaacagtt ggtgtaacag taatttcaat
      541 taaagcgcat aaaaatgcca caggtaaaaa tattagcagg taacacttta ctattgattt
      601 taataaaaca aaaaattttg gcaatgcctc ttttcttttt ctaaataaat tcattgaatt
      661 ttttctaata tacgcattta ataagaataa cattgatagt tcaatgcttg ttaagtatat
      721 ttctaaaata ccatgaggca aaataccaat aaaaatagct atcccttcgt ttaatttata
      781 tgaaaatgta accccaataa taaccgacaa tgaataaatt gtagaaatt
//
LOCUS       DAHHRR010000277          802 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.283, whole genome shotgun sequence.
ACCESSION   DAHHRR010000277 DAHHRR010000000
VERSION     DAHHRR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.283"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..81)
                     /locus_tag="NBI42_002758"
     CDS             complement(<1..81)
                     /locus_tag="NBI42_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCD1779092.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQF"
     gene            complement(267..689)
                     /locus_tag="NBI42_002759"
     CDS             complement(267..689)
                     /locus_tag="NBI42_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1779093.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
BASE COUNT          303 a          111 c          120 g          268 t
ORIGIN      
        1 aaattgaaaa attttagtct caaaaaggga gacgataaat aaaattaaaa agtaaattct
       61 agcgatgtat cgagattgca tcaataataa acactacttt ctaatgaaaa agttattcat
      121 tcaaatgtga tagttataaa cgcattcaat tcctaattaa aaatttaatt taactcgttg
      181 aagtatatta ataaacgaga atgcatttat gattaagcac ttatgataac ttgtaataaa
      241 atttgattca actaaaaagt aaagtgctat tgaggatcaa gtaaatttaa accgactgca
      301 tctttgaatg aacgttgaat gtccaaatca gttacagtaa gaatgatttc agcagttgca
      361 gaaacaattg ctttagcgag atgtccgcta aaagtttcat atgattgagt accgaaagta
      421 gcatgcaaat gtgcaaaatg accattgtct agacgagaaa tattacctaa taagctcgtc
      481 aattccagtg gctcagtaat atgtttttct tcgtattgtt tcgttgttaa attgaaaaat
      541 tttaatacaa cgtcatcaca tgcaccaatg ccgctgacag atgtaaatgt taagtcttgg
      601 tcatctgcaa aggttgttat acattcaacg atatcttctc ctttttccaa cactagtagt
      661 atagtatgat tacttttttg caatttcata tgattaatcc cctttatttt aatatgtcat
      721 taattataca attaaatgga aaatagtgat aattacaaag aaaaaatatt gtcaaatgta
      781 gcaatgttgt aatacaatat ag
//
LOCUS       DAHHRR010000278          795 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.284, whole genome shotgun sequence.
ACCESSION   DAHHRR010000278 DAHHRR010000000
VERSION     DAHHRR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.284"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(46..243)
                     /locus_tag="NBI42_002760"
     CDS             complement(46..243)
                     /locus_tag="NBI42_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779094.1"
                     /translation="MRIARAYGHKVKVILKDNNIIIGNVINFENPIESDTGNFVMDLD
                     TSMGIYSIDDSEIKDIQIINK"
     gene            complement(243..658)
                     /locus_tag="NBI42_002761"
                     /pseudo
     CDS             complement(243..658)
                     /locus_tag="NBI42_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373826.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
BASE COUNT          259 a          120 c           82 g          334 t
ORIGIN      
        1 gtataaaaat agcacctctt tctctttatc tgtgtagaaa ggatgctatt tattaattat
       61 ttgtatgtct ttaatttcag agtcatctat agaatatata cccattgagg tatctaaatc
      121 cattacgaaa tttccagtat cactttcgat tgggttttca aaattaatga catttccaat
      181 tataatatta ttatctttta aaataacttt aactttatga ccataggccc ttgctattct
      241 cattattcaa cattcttgtt aacatattga accacaagtg ttcctgttct aaaataatat
      301 actttttcaa aagttgtatc tatataatta ccttctacaa atgattttcc aactataatt
      361 tcaaaatctg ttatttcttt ttggttaaac ttatctttta ataataaaat ttcaccagtt
      421 cctactttgt ccgttaataa ttcattaagt atttgatttg atacagtagt ataacttagt
      481 aacaaataaa tatttatttt tgcttctttt acttctaagg atgaatatac ggcgcattca
      541 cacctggtat catatcttta actttaaata ttttgccgtt gagtgaatgt cataatttag
      601 atgttttact atctattttg ccaacgtatt tatattagct attctccatc aagtactttt
      661 agatacttta ttttcaataa aaaagtataa taaaatagca tctctcttta tctgtgtaga
      721 aaggatgctt taataccatg catatatata ttcattcgga aatttatttt catcaataaa
      781 atttaatatt gcatg
//
LOCUS       DAHHRR010000279          788 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.285, whole genome shotgun sequence.
ACCESSION   DAHHRR010000279 DAHHRR010000000
VERSION     DAHHRR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.285"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..148)
                     /locus_tag="NBI42_002762"
     CDS             complement(<1..148)
                     /locus_tag="NBI42_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LlsX family protein"
                     /protein_id="HCD1779095.1"
                     /translation="MRRIMMLKRQEMRVIIEILAGIVLSAIITAIAISTGYFIINKNG
                     YANFD"
     gene            complement(145..>788)
                     /locus_tag="NBI42_002763"
     CDS             complement(145..>788)
                     /locus_tag="NBI42_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1779096.1"
                     /translation="VIILSYGNFSIGNGRHFIDKYFLISTSIGLLPIAFISFPIWLSE
                     SIENETLKRLKYFEVNISSLIVSNIISYCFLAIFSVILNIIIGKVFFSLTLPNTQYFI
                     AFFFQIFYNIIALLMIGTFLALLIRKSKVLLPLGMILLFLTYMIIGVFVQYGELPNVL
                     KEIGNWIPIKYVTNDFYSIWINERMWDASFLKLNSLYIIVTSVLSLFIYKFKK"
BASE COUNT          322 a          132 c           81 g          253 t
ORIGIN      
        1 catcaaaatt cgcataacca tttttattga taataaagta accagttgaa atcgcgatgg
       61 ctgtgatgat ggcggataac acaatacctg ctaaaatttc aataatcact cgcatttctt
      121 gtctcttcaa catcattatt ctcctcattt cttaaactta taaataaata aacttaaaac
      181 actcgtcact atgatataca atgaatttaa ttttaaaaat gatgcatccc acatacgttc
      241 atttatccaa attgaataaa aatcattagt aacatatttg attggtatcc aatttcctat
      301 ttcctttaaa acattaggga gctctccata ctgaacaaaa acaccaataa tcatataagt
      361 aagaaaaagc aatatcattc ctaaaggcaa taaaacctta ctctttctta ttaataaagc
      421 taaaaatgta cctatcatta atagagcgat aatgttataa aaaatttgga aaaagaaggc
      481 tataaaatat tgcgtgtttg gtaaagttaa tgagaaaaat actttaccaa taatgatatt
      541 taaaataaca ctaaaaatcg ctaagaagca gtatgaaata atattagaaa cgattaaact
      601 actaatattt acttcaaaat attttaatcg cttcagcgtc tcattctcaa tgctctcact
      661 taaccaaatt ggaaaactaa taaaagcaat tggtaacagt ccaatactcg tactaattaa
      721 aaaatattta tctataaagt gtcttccatt tcctatacta aaatttccat aagacaaaat
      781 tataacta
//
LOCUS       DAHHRR010000280          772 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.286, whole genome shotgun sequence.
ACCESSION   DAHHRR010000280 DAHHRR010000000
VERSION     DAHHRR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.286"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            399..772
                     /locus_tag="NBI42_002764"
                     /pseudo
     CDS             399..772
                     /locus_tag="NBI42_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          308 a          115 c          121 g          228 t
ORIGIN      
        1 ctcagcctcg gtcttcgact ggcactgctc cctcaggagt ctcgccatta atactacgta
       61 ttaacatgta attttacttt taaatacttt aaaaaaataa gacatgaatc gtctacactt
      121 aattggacaa attctatgag aatagatatt gttaatttaa gaaagtaggc tattttatta
      181 tgacaagaga aagaaaatca tttagttcag agtttaagtt acaaatggtt agattatatg
      241 aaaatggtaa gcctaggaaa atggataaag caacatcaaa acacgggtac attcaatcac
      301 caagataact aatcaaatga agaaaaagag ctgattaaat tacgtaaaga agttcaacat
      361 ttaaaaatgg agaacgatat tttaaagtaa gcagcgctga tcatgatgct acttttatga
      421 gaatgaagga agaccatatg aaaaatggcc aacttaagcc aggatacaat ttacaaatag
      481 cgacaaattc tcaatttatt ttatcctacg acctatttca aaacccgaca gatactagaa
      541 ctttaattcc atttttaaca ctcaaaattg gaaatatgac gaacttaatg atgaatttat
      601 atgtcctaat aacaaaagaa taggttttaa aggatatgca taccgtaatg atagatatgg
      661 ttttaaacgt gacttcaaac tatatgaatg tgatgactgt tcatcatgtt ctttgagaca
      721 tcaatgcata aagccaaatt cgaaatccaa taagaaaatt atgaagaatt at
//
LOCUS       DAHHRR010000281          724 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.287, whole genome shotgun sequence.
ACCESSION   DAHHRR010000281 DAHHRR010000000
VERSION     DAHHRR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.287"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..412)
                     /locus_tag="NBI42_002765"
     CDS             complement(<1..412)
                     /locus_tag="NBI42_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1779097.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNM"
BASE COUNT          261 a          101 c          105 g          257 t
ORIGIN      
        1 ccatatttgt aattctactt gttacatctt caggtaattt atccatttga gctagtgcga
       61 atttaataat ttgctctgct tctttttgaa gggcaggtgt ttgactaatt aaattgttga
      121 tatttgaaat gatgattggt gcaacaaacg caacaattaa gccgataata gcaaacaaag
      181 ctaacatgat tgttgttata ctagcccatc ttggaaaacc gactttttca agtaagtttt
      241 gaaaaggtag acagatataa aataaaaaac cactaattaa aaatggaaga aatacagaac
      301 cgatgattgt agctattgga gtaaatactt cgtgcacttc cataaatagt ttgatgagaa
      361 tgaacagcat aatcagagca atgccagttc ggaaccaaac cttgtttaac ataaacgatg
      421 ttcctcctat ttttctaaac ttagttatta tttagtatat cctaaatagc agtgcttaaa
      481 aaggttaata ttttttaaat atacttaaat aaaccattca ctttctattt taatatattt
      541 actttcaata gtttgtgaga cttttcaatg actcgtaagt ctgcaaatgg atcaaacagt
      601 accaatttat atgaataact tattttaata ttaaacgtgt ttaaataatg aattgatcat
      661 atcattattt ataagaatat tctgaaaaat atattgaagt gtctcgtgct taatcgtata
      721 atat
//
LOCUS       DAHHRR010000282          706 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.288, whole genome shotgun sequence.
ACCESSION   DAHHRR010000282 DAHHRR010000000
VERSION     DAHHRR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.288"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..112
                     /locus_tag="NBI42_002766"
     CDS             <1..112
                     /locus_tag="NBI42_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1779098.1"
                     /translation="KYEEVLKVFDNDEMAVIVEFLNRSIEELKKEDGLND"
     gene            105..>706
                     /locus_tag="NBI42_002767"
     CDS             105..>706
                     /locus_tag="NBI42_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1779099.1"
                     /translation="MTNSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     IQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKY"
BASE COUNT          233 a           86 c          126 g          261 t
ORIGIN      
        1 aaaatatgaa gaagtactta aagtctttga taatgatgaa atggcagtta ttgtagagtt
       61 tttaaatcgt agtatagaag aattaaaaaa agaagatggt ttaaatgact aatagtaata
      121 tcagattata tattctagga tttttagcat ttttcgcatc attgattcaa aacatataca
      181 cgcctattat tccgagacta tatgacgatt ttcaagtacc actattttgg ataaatgcga
      241 cagttggcgg ttttatattt atcgttgcag ttatgcaaat tgtacttggt agaagtattg
      301 attctcgtga ttcgaaaaaa gtacttttaa cgggattggg aatagtaatc atatctagct
      361 ttatttgtgc agtgacacat aactttatat tatttgctat atcaaggtta attcaagcaa
      421 ttggttgcgg aattattcct ctcgtgactt taactttatt agccaaacta agtacagata
      481 atggtagagc tcaagctatg gcgaactatc aaatattttt atcatgtgcg ccagctttgg
      541 caccaatctt agggagtact ttaggcgcaa gatgggatta tattggtatt tttagttttt
      601 tgctcgttat atctatcgta ttatttctga ttatcttttt tattgatatt ccaaatgttg
      661 aaaaagggat tgtaaaatta actgaaaaaa ttgaagaaaa atattt
//
LOCUS       DAHHRR010000283          693 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.289, whole genome shotgun sequence.
ACCESSION   DAHHRR010000283 DAHHRR010000000
VERSION     DAHHRR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.289"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            278..528
                     /locus_tag="NBI42_002768"
                     /pseudo
     CDS             278..528
                     /locus_tag="NBI42_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
BASE COUNT          266 a           92 c           82 g          253 t
ORIGIN      
        1 aattttttaa cactgttaaa agttctttta attttgatta actaattaat ttacaatacc
       61 taaaatgttg tttggctttg tttataccaa gcttcaaact taaacgtcat aacaacattc
      121 atttcttaat tccgattaca tttgtcgatt atatttacag catctttata ctcaaaaaac
      181 atttacttaa aaatataaat tcgatttaat aattaattta aatttagtta atcaattttg
      241 catctatttt gttgtaagct atataaaagg agtgataatg atggtgaaaa aaacaaaatc
      301 caattcacta aaaaaagttg caacacttgc attagcaaat ttattattag ttggtgcact
      361 tactgacaat agtgccaaag ccgaatctaa gaaagatgat actgatttga agttagttag
      421 tcataacgtt tatatgttat cgaccgtttt gtatccaaac tggagacttt taacataaaa
      481 ttacttatca ttcaaaaagt aaaacagcat aatatcaagg tttataactt tatcattatc
      541 aataatacct catataaaat aaaattttag ggacttttta gggactttaa atttaaaatt
      601 acaagtttaa tagaaacatc aaaataatca catgtttgtg tggaatgtac accccaaaag
      661 ctagactgaa aaatctattt tttgaggtgt att
//
LOCUS       DAHHRR010000284          662 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.290, whole genome shotgun sequence.
ACCESSION   DAHHRR010000284 DAHHRR010000000
VERSION     DAHHRR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.290"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            44..331
                     /locus_tag="NBI42_002769"
     CDS             44..331
                     /locus_tag="NBI42_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779100.1"
                     /translation="MEKGIVNYDDANILKLDMNQVRCNKLVVDDIFKDYEQIKPTIEI
                     EKGNAILKLNGYFVASILETLNLNRVKKLYVDEDYYYTYNELIVKYTEVKE"
     gene            337..>662
                     /locus_tag="NBI42_002770"
     CDS             337..>662
                     /locus_tag="NBI42_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HCD1779101.1"
                     /translation="MKKVYMMVHELDVNKGGMTSSMFNRSREFYDANISADIVTFDYK
                     GNYDEIIKNLKKQGKMDSRTKMYNVFEYFKQISNSKHFKSNRLLYKHISERLKNTIEI
                     EESKGIS"
BASE COUNT          280 a           49 c          114 g          219 t
ORIGIN      
        1 tataaaatat attttgttaa atatttttat taagaggtgc acaatggaaa aagggattgt
       61 taactatgat gatgccaaca ttttaaaatt agatatgaac caagtaaggt gtaataaatt
      121 agtagtcgac gatatattta aagattatga gcaaataaag ccaacgatag aaatagaaaa
      181 gggaaatgca attttgaaat tgaatggtta ttttgttgca tctattttag aaacgttgaa
      241 tttgaataga gttaaaaagt tatatgttga tgaagattat tattatacat ataacgaatt
      301 aattgttaaa tatactgagg taaaggaata ataaatatga aaaaagtata tatgatggtg
      361 catgagttag atgttaataa aggtggcatg acctcttcta tgttcaatag aagtagggag
      421 ttttatgatg ctaatatatc agctgatata gttacttttg attacaaagg taattatgac
      481 gaaattatta agaatttaaa aaaacaaggt aaaatggatt caagaacaaa aatgtataat
      541 gtgtttgagt attttaagca aatatccaat agcaaacact ttaaatctaa tagattatta
      601 tacaaacata tttctgaaag attgaaaaat acaattgaaa ttgaagaaag taaaggtatt
      661 tc
//
LOCUS       DAHHRR010000285          658 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.291, whole genome shotgun sequence.
ACCESSION   DAHHRR010000285 DAHHRR010000000
VERSION     DAHHRR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.291"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..147
                     /locus_tag="NBI42_002771"
     CDS             <1..147
                     /locus_tag="NBI42_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779102.1"
                     /translation="KEISSYQINKDTGISYGNINAMRRGERRIENLSLKNAKILYEYA
                     KKVL"
BASE COUNT          269 a           66 c          119 g          204 t
ORIGIN      
        1 aaagagatat cgagttatca aattaataaa gatactggga taagttacgg taatattaat
       61 gctatgcgcc gtggggaaag aagaatagaa aatttaagct taaagaatgc aaaaatctta
      121 tatgaatatg cgaaaaaggt attataatat aagcattgat ttcaataggc atacaataac
      181 tgcccaaact agtgactgag agggtgcttt tatagtatta aaagttttct aaaagtgaaa
      241 gaaatcatgt ttagtaattt acttatgttt tacaaaatag tataatactt ttatataata
      301 tatagagctt cagtatacct tttgagtata tatggagttc tttcttttta tacaagatgt
      361 atatgtataa atggaaatgt ttaatacatt acataaaaac cttggatatg ggaattcaaa
      421 catgtcactt accccaattt gtggaatgtt aaataactat aaagaggaaa ggagaaaaaa
      481 tgataagaat ggttaaagaa aaagaacgaa tagcaaaatt aaatttcgaa tagctgtgac
      541 gagcataata attcaagtac ttaattttat caaatcgttc ttttaaaagg gtaaagggag
      601 gaagcccttt accacattaa attgggaggt ggtatttatg acaagagaat taaggaaa
//
LOCUS       DAHHRR010000286          643 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.292, whole genome shotgun sequence.
ACCESSION   DAHHRR010000286 DAHHRR010000000
VERSION     DAHHRR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.292"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            80..598
                     /locus_tag="NBI42_002772"
     CDS             80..598
                     /locus_tag="NBI42_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCD1779103.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYNKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
BASE COUNT          251 a           94 c           77 g          221 t
ORIGIN      
        1 tattatgaaa atgaattatg ttatagtatt ccagacttta tcgtgattaa attaattttc
       61 aatatcaaag gagtttatca tgtattcaaa tgttcaacca ttcattaata gtgatttaac
      121 taattgtttt caatcacaat acactgaaat tgaatcatta ttattcaacc tcttagacag
      181 cccagtaaaa tatacaaaac atattggcgg tacgcatcat tttaaatatt caacagagcc
      241 aattttagat attttagtag gtgtagaaaa tttgcatgat attacatcct tagatgaaaa
      301 acgcttaaat tatgtagggt tttatcgttt gcatcataaa tataataaaa aggttatgat
      361 ggcgaagttt aacaattttc atgaactaaa acaacaggtg cgtctacata tcattcaaat
      421 gaatacccaa acatttgaat cttatcttgc acttgattat ctcttatcaa ctaatgatga
      481 tattgctttg caattcaata caaaaaagca ccaaatattg caacatagct taacaattag
      541 acaatatgag atgcaaaagc aacaatactt tgaaaaacta tgtaaaaaat acttataacg
      601 acaataatgc cgtattttat aattatacta attctaatac ttt
//
LOCUS       DAHHRR010000287          642 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.293, whole genome shotgun sequence.
ACCESSION   DAHHRR010000287 DAHHRR010000000
VERSION     DAHHRR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.293"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            197..355
                     /locus_tag="NBI42_002773"
     CDS             197..355
                     /locus_tag="NBI42_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779104.1"
                     /translation="MITIDSKINKQIAKNLSLEGMYVTREQQIQILHAINNKKEITNE
                     LIRKIAFK"
BASE COUNT          262 a           66 c          102 g          212 t
ORIGIN      
        1 atcatcactt ttaaaagttt attagcgaaa tagatatgta agtcttaaaa gtgattagcc
       61 gtataaccgt aattgttatt tatgaaagtt aattttgtgg ttgaatgaac aattttcaaa
      121 gtagatcatt atgcaagtca taaataaaac agttagttga taaacgatat aaaagtgata
      181 tataggaggc gatatcatga ttactattga ttcaaagatt aataaacaaa tcgctaaaaa
      241 cctttcactt gaaggtatgt atgttacacg agaacagcaa atacaaattc ttcatgcaat
      301 taataataag aaagaaatta caaatgagct tattcgaaaa atcgctttta aataatgaaa
      361 tatgatggaa tacagcaaga cgaatattta ttaaattcga atatggttgg tgccaaaact
      421 tctgatgaat taaagcgact tggaaaagtt acttttttta attgttgaat ctttcgtcga
      481 agaggaagga tataaattct atttccgtat cgtttaaagt gaattacaag ataattatca
      541 aaaaaagcat atatactata ttaaaagtgg catagggaca gtcattaaaa tataagagga
      601 ttattaaagt gcaatttgtt gtaatataat aattaaattt ta
//
LOCUS       DAHHRR010000288          641 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.294, whole genome shotgun sequence.
ACCESSION   DAHHRR010000288 DAHHRR010000000
VERSION     DAHHRR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.294"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
BASE COUNT          262 a           76 c           91 g          212 t
ORIGIN      
        1 taattggaag tacatttttt attgagagag agttaagtaa aaaatattcg ttacaggaaa
       61 aagctaaaaa gttatgtttg tcgttactac ctattaatat catagttgtc tttatattct
      121 ttatatttat actgtgaata aaaatatatc tagcctgaaa tagaaatgtc atagtctatg
      181 taaaagacaa tctccattag aactaagatg tgcatcccaa aagttggact aaaaaactaa
      241 ctttatgggg tgtattttta tgctaatcat cataaattcg agattatgtt aaaggtagtt
      301 caagagtaat taaacaataa attaaaaata gtaggatact tattttgagg gaagaaaatt
      361 aactgtatat atataatgta ggaacaagcg taatggctta tcctaataca atcattgtgg
      421 atgggatgat attttaagat ttaaaatacg acaccagcaa acattataac tgtaatagct
      481 cataaatccc tccatacagc taatctaaaa aaataataca tcattggaat caagcctcaa
      541 gcatgtaaat attaaaaatc acaaaagata tatgtaaaaa agttacaatt tgcataatta
      601 aattgtgtct aattattgac taattaaatt ttgccaaata t
//
LOCUS       DAHHRR010000289          636 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.295, whole genome shotgun sequence.
ACCESSION   DAHHRR010000289 DAHHRR010000000
VERSION     DAHHRR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.295"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(71..565)
                     /locus_tag="NBI42_002774"
     CDS             complement(71..565)
                     /locus_tag="NBI42_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1779105.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
BASE COUNT          220 a           85 c           91 g          240 t
ORIGIN      
        1 gagtagttgt atgaaaaata gaacctatat gatttgaatt tatgagaaat gtagaatata
       61 taaaactgta ttacttatta atcaaatcat aaaaaagcca atgttcatcg aaataggtat
      121 ttccgatttt aattgcattt ttctcaaaac caagaatgtc gaatcctagt gcactataga
      181 aaactttagc acctatgttg tttgacacaa ttgatgtaag taaagattcg taattgttct
      241 gccgtgcata attaataatg aaattaatga gctcgctatt aatcgattta tcgttattag
      301 taacaaagtt gtatttaatt aatgatttat gttcttttcc aacgtatcga atttgttcta
      361 aggcagctgt tgcgataagt gtttcgtttt cataacagcc aaatacaata catttcggtg
      421 aaccttgatt taaaatgtca tgtattaagg tgtcagtcat tgtttgacta taatgtgaat
      481 cttgagtgaa ttcttcattg actgtcgaaa caagtctctt atactcatta aaatcattat
      541 tcgttaatgt tttaatttca atcattactg acctccttaa tcattattaa gaaagatttt
      601 gtactaaaaa aatttcttaa ttacatatta aaatgt
//
LOCUS       DAHHRR010000290          622 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.296, whole genome shotgun sequence.
ACCESSION   DAHHRR010000290 DAHHRR010000000
VERSION     DAHHRR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.296"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            25..522
                     /locus_tag="NBI42_002775"
     CDS             25..522
                     /locus_tag="NBI42_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779106.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
BASE COUNT          216 a           88 c          112 g          206 t
ORIGIN      
        1 ctggagaaat aggaggacat tgccatgcaa caccttataa aaaaacatgt attgaatggc
       61 gagtttgatt tagtacgaca attgatgtcc gaaacagatt ttatggaatt tgaagaagca
      121 tatatttcaa gtgcgcatga agtagaaagt atgatgtttt atacatgtat tttagatatg
      181 attaagtacg aagaatcatc tgaaatgcat gacttagcat ttttattgct tgtgtatcca
      241 ctaagtgaat atgaaggtgc tttggattct gcttattatc atgcagacgc ttccataaaa
      301 cttactgacg gcaaagaagt taaaagtttg ttacaaatgt tattattgca tgcgatacca
      361 acacctgtta tttcagataa gaaggctttt gatatcgcca agcaaatttt aaaattagat
      421 cctaataata atgttgctcg taacgtctta aaagacactg ccaaacgtat ggacaacgtt
      481 gttgttgata taaatgaatt acaccaacgt aatgcacgtt aattacattt caattatatt
      541 agcttaataa tagttttaac atttggttgg gttgggacat atgttccagc cttttttaat
      601 acttaaaaac taacgaagta aa
//
LOCUS       DAHHRR010000291          614 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.297, whole genome shotgun sequence.
ACCESSION   DAHHRR010000291 DAHHRR010000000
VERSION     DAHHRR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.297"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
BASE COUNT          215 a           91 c           73 g          235 t
ORIGIN      
        1 caacattaaa ttttatagaa aaatccagtt cacactctag ttctgtaatt aatattccat
       61 tctttgagcg actaaaaaac ttattaattc aacaattcga aaatgttcat tccatagcag
      121 acttggattt taaacttaat ctaatgctca atgcattttc ttcagatttc tattattttg
      181 taaaacataa ccaaaaatta acaaaacaac aatttttaga taaattacta gatttatttc
      241 taaaataata tgttctgaaa aatagaagta ttttttgaac cactaaataa cgcgctatta
      301 aatggcttcg ctatgcccgt ctaacacatt agtgtaaaat tttataaatt gaatttttga
      361 tgacgggtcc cttcctagtg tgttctctca gacacccaac cggcacatta atgtaagttg
      421 acttttcgat aatattaaag atttaaaatt ataaagctat taattatggt tagtggtttc
      481 aaaatgttat tttttagagg tttaagattg ccatgaattt agttttattt gttctcatgt
      541 tcaggtttct ttatatcaac ttttatttta gtaaactaaa aagagtagca acttcaaaat
      601 tcgcatttat atct
//
LOCUS       DAHHRR010000292          608 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.298, whole genome shotgun sequence.
ACCESSION   DAHHRR010000292 DAHHRR010000000
VERSION     DAHHRR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.298"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>608
                     /locus_tag="NBI42_002776"
     rRNA            <1..>608
                     /locus_tag="NBI42_002776"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          162 a          132 c          178 g          136 t
ORIGIN      
        1 tcattggaaa ctggaaaact tgagtgcaga agaggaaagt ggaattccat gtgtagcggt
       61 gaaatgcgca gagatatgga ggaacaccag tggcgaaggc gactttctgg tctgtaactg
      121 acgctgatgt gcgaaagcgt ggggatcaaa caggattaga taccctggta gtccacgccg
      181 taaacgatga gtgctaagtg ttagggggtt tccgcccctt agtgctgcag ctaacgcatt
      241 aagcactccg cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacggggacc
      301 cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaaatct
      361 tgacatcctt tgacaactct agagatagag ccttcccctt cgggggacaa agtgacaggt
      421 ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc
      481 aacccttaag cttagttgcc atcattaagt tgggcactct aagttgactg ccggtgacaa
      541 accggaggaa ggtggggatg acgtcaaatc atcatgcccc ttatgatttg ggctacacac
      601 gtgctaca
//
LOCUS       DAHHRR010000293          604 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.299, whole genome shotgun sequence.
ACCESSION   DAHHRR010000293 DAHHRR010000000
VERSION     DAHHRR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.299"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            124..>604
                     /locus_tag="NBI42_002777"
     CDS             124..>604
                     /locus_tag="NBI42_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCD1779107.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKDKTAQAMFI
                     "
BASE COUNT          283 a           67 c           86 g          168 t
ORIGIN      
        1 tttaatatca tcttagaatc tcacaaaata tcaactttgt ttaattactc aaaacgttaa
       61 gcaattttaa atcgttacga gtttttaaaa ataaattttt gcgaaataaa ggagacatgt
      121 taaatgaaaa aagtaatggg gatattatta gcaagtacac ttatcttagg tgcttgtgga
      181 catcatcaag atagtgcaaa aaaagagagc actagtcaca aaaagaaaga aaatgacaat
      241 gaagaattaa atgaagaact caaagaattt aaaagcaaaa aaaatatgga tataaaaatt
      301 aaaggcgata ctattgttag tgacaaattt gaagctaaaa taaaagaacc gtttatcatc
      361 aatgaaaaag atgagaaaaa gaaatatatc gcttttaaaa tggaaattac tgctaaaaaa
      421 gacgataaag atttaaatcc atcttctatt tctcatgact atattaatat cactcaagat
      481 gataaaaata cagtaaataa attaagagat ggttatcttt taagtgataa aaaatataaa
      541 gattggacag aacataatca agatcaaata aaaaaagaca aaactgcaca agcaatgttt
      601 atct
//
LOCUS       DAHHRR010000294          586 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.300, whole genome shotgun sequence.
ACCESSION   DAHHRR010000294 DAHHRR010000000
VERSION     DAHHRR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.300"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(108..542)
                     /locus_tag="NBI42_002778"
     CDS             complement(108..542)
                     /locus_tag="NBI42_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1779108.1"
                     /translation="MFSKVNNQEMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPISETTVKIERVAVIKSHRRQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
BASE COUNT          183 a          109 c           78 g          216 t
ORIGIN      
        1 atgaaaaaaa ttttgaatct caatggtgac gtgacaaatc aaatagaatt gatgtcaatt
       61 taagaattgg tttattacaa atgtttctaa aatagataca acttttttta attaagcgag
      121 gttaactttt ttgtcatttc aatatgctcg atgccttcct caagaaatat attacctctc
      181 attttaaagt ttaaactttc ataaaatggg atagcatgac attgggcatt catagtagct
      241 acgtaaaaac cttcatcttt agctaatgat tctacagctt gcataagcat tctacccatt
      301 ccttgtctac gatgtgattt tatcacagcc actcgttcta ttttgacagt tgtttcacta
      361 atagggcgta tacgagctgt ggcaactggc tgtccattat catatccaat gaggtgaata
      421 gattcagatt catattcatc aatttcactt tcctctggga cgccttgttc ttctacaaac
      481 actttctttc ttatatagaa gcaatcttct aacatctctt gattgtttac ttttgaaaac
      541 ataaaattcc tccatatcct aacaaaatca tttacattta atttac
//
LOCUS       DAHHRR010000295          585 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.301, whole genome shotgun sequence.
ACCESSION   DAHHRR010000295 DAHHRR010000000
VERSION     DAHHRR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.301"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>585
                     /locus_tag="NBI42_002779"
     CDS             <1..>585
                     /locus_tag="NBI42_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779109.1"
                     /translation="NSEKKDQDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFI
                     LISYLYDKQYVQFQAITGMSLFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIA
                     IISLLLIILQAFNITWGVIPITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQK
                     TVDAMIHNPQSAQIFSLIIWILLVVLVIYFTIRLS"
BASE COUNT          195 a           78 c           80 g          232 t
ORIGIN      
        1 aaattctgaa aagaaagatc aagattatat tgaatataaa agaaaatctc cagcacaaat
       61 catattaatg attcaaagga atttgtttgc tttacaaatg aatccagtta ttttctttat
      121 tataaacttc attctcatat cctatttata tgataaacag tatgttcagt ttcaagctat
      181 tactggaatg agtctatttt attgtttagt gatttttcca atgactattg ttgtatacct
      241 aagggtgtct caaaagaatt acttgcgtag taataaaata gaaatgatta tgggtacaat
      301 tatcgctatc atatccttgt tattaattat attacaagca tttaatatta cttggggcgt
      361 tataccaatt acaaattttg gacatcaatt tttctttttc attggtatta ttttagtaat
      421 tgccggcata ttttataagc gacttgagtt ttcgggaatc ggcttattat tttgtcaaaa
      481 aaccgtcgat gcaatgattc ataatccaca atcagcccag attttttcat taattatatg
      541 gatattatta gtagttctag ttatatattt cacaattaga ttatc
//
LOCUS       DAHHRR010000296          585 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.302, whole genome shotgun sequence.
ACCESSION   DAHHRR010000296 DAHHRR010000000
VERSION     DAHHRR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.302"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(66..218)
                     /locus_tag="NBI42_002780"
                     /pseudo
     CDS             complement(66..218)
                     /locus_tag="NBI42_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494615.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
BASE COUNT          195 a           96 c           65 g          229 t
ORIGIN      
        1 tctacttcgc tcctttcatg cttactttta gtaccatttt cattattggt gtaaactttt
       61 gcgtcagctt tagcttcacc gctaccaata ccaaaaatta acgtagaacc tactaaaatc
      121 gaagctgtac cgatagtgaa ttttctaatc gaatatttgt tcaatcgatt agagagaaaa
      181 tctacacgct tgttaattgg accactcttt tttctcattg gactactccc tttcattatt
      241 tccaccataa aattcagtga atttacatga aattttacaa tcggcttcga ggtcgaacaa
      301 tacaatataa catttatatt tataaacttt ttttatattt tgcttaatat aaaccaatgc
      361 aaaaaaagag aaggttttta ttatttataa tacttaattt cttaatagcg attaaatctc
      421 aaaattcacg gtatcattat ctgtgtaagt acaattgatt cattgaaaca agaaacttaa
      481 taatgctaaa tttttaacta taaattattt cattcaaaat attctcaatt gttttttgtt
      541 gtagttcagt aagtgttaat attacatttc tactatcttt aagat
//
LOCUS       DAHHRR010000297          584 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.303, whole genome shotgun sequence.
ACCESSION   DAHHRR010000297 DAHHRR010000000
VERSION     DAHHRR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.303"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
BASE COUNT          225 a           91 c           59 g          209 t
ORIGIN      
        1 cgatactcaa ctttttcgct ataaaaatca aaacgaactt taatcaaatc ttaaaaaata
       61 atgtttgtat tatcacaaat aatctataat ttaatttaaa attaactgat tattaactat
      121 tgtccgttaa atggagatga aacaacttat gttaataact catatgaaaa caagtctgtt
      181 ataacttaat caatacgtat atgtacataa tgtcatttgc ttcatataaa aaatttaatg
      241 ctatgattta gccaaattaa aaacatatat atatttacta cgaactaaaa gttcacccta
      301 aaaaataaaa aaccatgatt tcgaagtctt cataaactga agcttctact ctcatggcaa
      361 tttatactat gatattcaat tataatgcct agtctcatag taaacggcta ctacattcgc
      421 atgtacttcg ttttactgtc cacgcatggt cgttgactta gttatattat aatataaata
      481 ttttattttt tcaatactta attttaaaag tcccctatga aaatgttgat gagcaacatc
      541 tttaaatcca atcatacctt gtatgaaaaa catattgtgg aatc
//
LOCUS       DAHHRR010000298          576 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.304, whole genome shotgun sequence.
ACCESSION   DAHHRR010000298 DAHHRR010000000
VERSION     DAHHRR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.304"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            21..>576
                     /locus_tag="NBI42_002781"
     CDS             21..>576
                     /locus_tag="NBI42_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD1779110.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNTNEASAAAKQLDKSSSSLHH
                     GYSKINVPYTITVNGTSTNILSSLTLNKNQNISYKDLENKVKSVLKSNRGISDIDLRL
                     SKQAKYTVHFKNGTKKVIDLKSGVYTANLINTSDIKAISVNVDTKKHVKDNNKVKANY
                     QVPYTITVNGTSTNILSSLTFKKNQ"
BASE COUNT          261 a           76 c           80 g          159 t
ORIGIN      
        1 caaaaaagga gagataattt atgaaattta agtcattgat tacaacaact ttagcattag
       61 gtgttttagc atcaacagga gcaaacttta atactaacga agcatctgcc gcagcaaagc
      121 aattagataa atcatcaagt tcgttacatc acggatattc taaaattaat gtaccatata
      181 caattactgt gaatggaaca tctacaaaca tattatcaag tttaacttta aataagaacc
      241 aaaatataag ttacaaagat ttggagaata aagtaaaatc agtcttaaaa tcaaatagag
      301 gaattagtga tattgattta agactatcaa aacaagcaaa atatacagtt cattttaaaa
      361 atgggacaaa gaaagtaatt gatttaaaat caggtgttta cacagcgaac ttaatcaata
      421 caagtgatat taaagcaatt agtgtaaacg tagatactaa aaagcatgtg aaagataaca
      481 ataaagttaa agcaaattat caagtaccat atacaattac tgtgaatgga acatctacaa
      541 acatattatc aagtttaaca tttaaaaaga accaaa
//
LOCUS       DAHHRR010000299          573 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.305, whole genome shotgun sequence.
ACCESSION   DAHHRR010000299 DAHHRR010000000
VERSION     DAHHRR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.305"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            91..>573
                     /locus_tag="NBI42_002782"
     CDS             91..>573
                     /locus_tag="NBI42_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCD1779111.1"
                     /translation="MLCEVIAIYKNPKYRIIKYNDEYLMVNIINNWLVLFIPLLNWLT
                     PKRYIKISQEELESLNTFKPAKNNAFWPALGSSVLFSVTFRKYMPLFNVRLEKTIVIA
                     IFFVVFLGILFFYLNLNRRLALSVFTMNKEKSQKMILLPNLKHFFFIIFAYLYFGGFS
                     F"
BASE COUNT          207 a           57 c           84 g          225 t
ORIGIN      
        1 atttttaatg atagtaagtt gataatattt taatgtcttt tgaattatcg aaaaatgtat
       61 tacacataaa attaaaggga tgtggagaca ttgctttgcg aggtaatagc tatatacaaa
      121 aatcccaaat atagaattat taaatataat gatgaatatt tgatggtgaa tataataaat
      181 aattggttag ttttattcat tccattgctt aattggttga caccaaaaag atacatcaag
      241 atcagtcaag aagaattaga aagtttaaat actttcaaac ctgctaaaaa caatgcattt
      301 tggccagcat taggtagttc agttttattt agtgtgactt ttagaaagta tatgccttta
      361 tttaatgtta gattggaaaa aacaatagta atagccatat tttttgttgt atttcttggt
      421 attttatttt tctacctaaa tttaaatcgt aggctagcat taagtgtttt tactatgaat
      481 aaagaaaaaa gtcagaaaat gatattgcta cctaatttaa aacatttttt ctttataata
      541 tttgcatatt tatatttcgg tggtttttca ttt
//
LOCUS       DAHHRR010000300          568 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.306, whole genome shotgun sequence.
ACCESSION   DAHHRR010000300 DAHHRR010000000
VERSION     DAHHRR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.306"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..434)
                     /locus_tag="NBI42_002783"
     CDS             complement(<1..434)
                     /locus_tag="NBI42_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1779112.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKS"
BASE COUNT          192 a          105 c           63 g          208 t
ORIGIN      
        1 tcagacttat taaaacccgc tatatcatct tgatattgaa ttaacaattg agtaaagctg
       61 tgtattaagt catctttctt cgatgcaatg gtagttaatg attttacacg ataagcatat
      121 ttatctgtaa ataacttagc aactacccca cctaaatcat gtcctagaat atgtgcctca
      181 tgaatattta acttctccat tagaattttt aaatcctcaa cgtgatcgtt taaatcgtat
      241 gattcacttt tagaagactt gccatgacct cttaaatcgt aagtaagtac tttatatgat
      301 gatgctagtt gatgttgcaa atcttcaaat ccagctaaat taccatccaa tccatgaagt
      361 aatattatag gatccccttt accttcaatt tgataagtta attggatttg aggattaatt
      421 gtgactttat tcattgtgac acctccctca ataaactata tacccaacct tcattctata
      481 tacgcttcca ttttataaac aattttttta aattatttat aaattaccaa aagttattcc
      541 aacaataaaa aaactattca tctcaatt
//
LOCUS       DAHHRR010000301          539 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.308, whole genome shotgun sequence.
ACCESSION   DAHHRR010000301 DAHHRR010000000
VERSION     DAHHRR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.308"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            100..>539
                     /locus_tag="NBI42_002784"
     CDS             100..>539
                     /locus_tag="NBI42_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HCD1779113.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTG
                     SKSTVVTPAMYKENLESLISSLSFADYEKYYEPEFKDVVKKYQQRFMAEDAALKKFFR
                     EEYFIKENKNSDEMLGITQERYKYIYDKLKSNNDEFMREIKEIK"
BASE COUNT          221 a           47 c          111 g          160 t
ORIGIN      
        1 taattatttt aaaaaggtag atgttatata atttggcttg gcgaaaaaat agggtgtaag
       61 gtaggttgtt aattagggaa aattaaggag aaaatacagt tgaaaaataa attgctagtt
      121 ttatcattgg gagcattatg tgtatcacaa atttgggaaa gcaatcgtgc gagtgcagtg
      181 gtttctgggg agaagaatcc atatgtatct gagtctttga aactgactgg gagtaaaagt
      241 acagttgtta caccggcaat gtataaagaa aatttagaat ctttaatatc atctttatca
      301 tttgcagatt atgaaaaata ttatgagcca gagtttaaag atgtagtgaa gaaatatcaa
      361 caaagattta tggcagaaga tgcagcctta aaaaaattct ttagagaaga gtactttata
      421 aaagaaaata aaaatagcga cgaaatgcta ggtattactc aagaaagata taaatatatt
      481 tatgataaat taaaatctaa caatgatgaa ttcatgagag agataaagga aattaaaga
//
LOCUS       DAHHRR010000302          537 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.309, whole genome shotgun sequence.
ACCESSION   DAHHRR010000302 DAHHRR010000000
VERSION     DAHHRR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.309"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            258..>537
                     /gene="sarX"
                     /locus_tag="NBI42_002785"
     CDS             258..>537
                     /gene="sarX"
                     /locus_tag="NBI42_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HCD1779114.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERK"
BASE COUNT          224 a           58 c           87 g          168 t
ORIGIN      
        1 caactacctt aaaccagcaa tcgagattaa agagcataat tttagtgata attccttgaa
       61 tattaacgtt acttttaatg cgttatacat aattaaaata ggaaaaaaat aacaccttga
      121 tatgtattgc aaaatttaat ttgcatttgt tggtaatttt gacaacgtat taaaaacaaa
      181 tgagaaagac aatggcagaa aaatttaaga taataatgac agaagcattg tctttatata
      241 tttgggggtg caacattttg aatactgaga aattagaaac attgcttggc ttctataaac
      301 aatataaagc attatctgaa tatattgata aaaaatataa gttgtcgcta aatgatttag
      361 cagtcttaga tttaacgatg aagcattgca aagatgagaa agtacttatg caatcatttt
      421 taaaaactgc aatggatgag ctagatttaa gtaggacaaa attattagtt tctataagaa
      481 gactaattga aaaagaaaga cttagtaaag ttagatcatc taaagatgag cgtaaaa
//
LOCUS       DAHHRR010000303          534 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.310, whole genome shotgun sequence.
ACCESSION   DAHHRR010000303 DAHHRR010000000
VERSION     DAHHRR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.310"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            412..>534
                     /locus_tag="NBI42_002786"
     CDS             412..>534
                     /locus_tag="NBI42_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCD1779115.1"
                     /translation="MKAFKGQKKTDYEFGKKLNEFIQSYVIILLIDIRLPVVRLY"
BASE COUNT          228 a           55 c           78 g          173 t
ORIGIN      
        1 atttacagta atttaaatat catgacgcat atcaatagtc aaaataataa ttttaaacgt
       61 aatccattta taaatgaaga acaattgaaa tcactactcg cgataaagaa agtagatgca
      121 catatgagtt atgattcaaa tattacttca aaagcattgg cagaaataaa caaaacgcaa
      181 aaaagattag ttacaaagat agacacactc tataatcagt caaaaaaaga aaatgaatat
      241 gtacggttag gagaaaaaat atcgtataca aaattggaga atcattggaa atatttatat
      301 aatgagattc agttttataa tcttaataat caattgatta gttatgttgc attagaacag
      361 gaatatgctt gggctttttt aaatgaacta ttttatttga tagaattgta tttgaaggca
      421 tttaaaggac aaaagaaaac agattatgag tttgggaaaa aattaaacga atttattcaa
      481 tcatatgtca ttattttact tatagatata cgattacctg ttgttagatt atat
//
LOCUS       DAHHRR010000304          520 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.311, whole genome shotgun sequence.
ACCESSION   DAHHRR010000304 DAHHRR010000000
VERSION     DAHHRR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.311"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>520
                     /locus_tag="NBI42_002787"
     CDS             <1..>520
                     /locus_tag="NBI42_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCD1779116.1"
                     /translation="LVEVDSSQINTYIKKLQMKEKELLNLEEVKIFNVSTENKRHQNA
                     QAKQIIRLFNAETSQLINKVNSKNIESMQNKIFKSYEGINKIFETDNVRIPETLLDIK
                     LEILDLMHKYQVKIEDEKIIRREERARLKEIEQAEKEMEKKLKELDKDIRHQNNEIKK
                     LTMYLNNTDLQVE"
BASE COUNT          257 a           58 c           79 g          126 t
ORIGIN      
        1 tttagttgaa gtagactcat ctcaaattaa tacatatatt aaaaaattac aaatgaaaga
       61 aaaagaatta ctaaatcttg aagaagtgaa aatatttaat gtttctacag agaataagag
      121 acatcaaaat gcacaggcca agcaaataat acgattattt aatgctgaaa cttctcaatt
      181 aattaataaa gtaaatagca aaaatatcga aagcatgcag aacaaaatat ttaaaagtta
      241 tgaagggata aataaaatat ttgaaacgga taatgtacgt attcctgaaa ctttacttga
      301 cataaaactt gagatactag atttaatgca caaatatcaa gttaagatag aagatgaaaa
      361 gatcataaga agagaagaac gagctagact gaaagaaata gaacaagcag aaaaagaaat
      421 ggagaaaaaa ctaaaagagc ttgataaaga cattagacac caaaataatg aaatcaaaaa
      481 gctgacaatg tatttaaata acacagattt acaagtcgaa
//
LOCUS       DAHHRR010000305          517 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.312, whole genome shotgun sequence.
ACCESSION   DAHHRR010000305 DAHHRR010000000
VERSION     DAHHRR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.312"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..474)
                     /locus_tag="NBI42_002788"
     CDS             complement(<1..474)
                     /locus_tag="NBI42_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD1779117.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSL"
BASE COUNT          201 a           88 c           82 g          146 t
ORIGIN      
        1 taatgaatta tagtaaattt ttgtttcaaa tttaggtggt agaaacacaa agttaactag
       61 agatgaactt atgacaccaa tcattacaag tacaaacctg taaaaggcgg taatatagaa
      121 agaaccggta tgttgtccca taatgattaa tgctgttaca ctcgccaaag tagcaacgtg
      181 tgctaaatta aatttaaata aaatagcaat aagtactatg acggtaacac ccataatgat
      241 aaaattatcg ctaaaaattg ttaccattgt aacagaaagt atggcaccta taatgttacc
      301 taatgcttga tcagaaactg ttttaaatga acgataaata ctaggttgca ttgcaacaac
      361 agcactgaca ccagctaagg ctttcagacc aacatcatcc ggtagtaaag aagcgataga
      421 catagctaaa ataatggcta taccagtttt aaaaatccga gctcctagtc tcaaaaataa
      481 tgacgcccct tttaagttta ttgaatatct aatattc
//
LOCUS       DAHHRR010000306          502 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.313, whole genome shotgun sequence.
ACCESSION   DAHHRR010000306 DAHHRR010000000
VERSION     DAHHRR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.313"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
BASE COUNT          211 a           63 c           66 g          162 t
ORIGIN      
        1 aaacattaca aaaaacaata ccaaatttga accgataaga taaaatatct tgtcggtttt
       61 taatataaca agtgatattt tggaaatgcc aaaaatatat agattgttta tataaaatga
      121 tttaaaatgt tttataacaa catattttat gcaacacata aaatgggtaa ctaacatcac
      181 gaaatcattg tatttgttgt tacactgatg aagcgatgtc acaaagaaat gtcgttatca
      241 aacgaagccc gaaatacata ctgatactcg aagtaatgta acgataaata acaatgataa
      301 cgattgatta ctacgcaaaa atatatgtaa attgacgatt taaatgcaat tcgtatattt
      361 tgccaacgat ttacacatta aaatgtttga aaattgtcac aattcagtct aatttaaaag
      421 gaagttaatt acaaaattca aaaatcatgc atcgtgtcat ttagatattg aaaaagatga
      481 gtcaaatttg tttcatatat tt
//
LOCUS       DAHHRR010000307          498 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.314, whole genome shotgun sequence.
ACCESSION   DAHHRR010000307 DAHHRR010000000
VERSION     DAHHRR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.314"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            43..495
                     /locus_tag="NBI42_002789"
     CDS             43..495
                     /locus_tag="NBI42_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779118.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEMNENAKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
BASE COUNT          204 a           57 c           86 g          151 t
ORIGIN      
        1 gttaagatga taaaaaatga gtaaggaaat gtgggtaagg ggatgacagt aaaaaattta
       61 tttttaggct ttgttgctgt aatattaacc gtatgtttga ttggtttatt aatattagca
      121 acaaatgaag atgcgcttgc taaggtacat aaaacgatta atacgcttaa cgcgataaat
      181 gtatcaactg aagatactta taaaaagaaa atggatattc tcaatattca tactgctaaa
      241 gcatctgaaa tgaatgaaaa tgcgaaaaag caaaatcatt ttaaacatcg tgtgaatgca
      301 aataaatcaa attcttttaa cgaacaagag tgccaagtta ttgctgatcg ttatgcagat
      361 aagcatatca atgataatta tggtctagaa agaatttcta agacaaatca tggatataat
      421 tatgtttatt ccaatgataa ttcaactagt aagcaacatg taagtatttc aaatcaaggc
      481 ataataacga aataatag
//
LOCUS       DAHHRR010000308          492 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.315, whole genome shotgun sequence.
ACCESSION   DAHHRR010000308 DAHHRR010000000
VERSION     DAHHRR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.315"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
BASE COUNT          191 a           61 c           52 g          188 t
ORIGIN      
        1 aagcaaatga aaattagata atctaattat ttcaacatta aacaatatat cattataaag
       61 aagttttcaa aatagaagta taaggtggtg aacaaaaatt aacattgtgt ataacattat
      121 agtatatata gcatgcttga ttaatttcgg attaattata gtcccattat taacaacaaa
      181 tgatgcggat tttgtatgga cttcttcatc attaatatat atgattcttc ttacgtcgat
      241 tatcctaata gtttatatta aaactaaaga tagcttacag ttaacgatat ttatacttaa
      301 tatagcttta attattctat actgtgcacc attactattc atttatgtat tctagtgctt
      361 cattaatatt ttaatgcatc agtttaagta aattacattt ggaaaataaa aagtaaatat
      421 catcatcaaa ttaacatatt taacgttata ctttcataaa atttgaaaca attgaacatc
      481 cttactttag ct
//
LOCUS       DAHHRR010000309          490 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.316, whole genome shotgun sequence.
ACCESSION   DAHHRR010000309 DAHHRR010000000
VERSION     DAHHRR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.316"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            112..483
                     /locus_tag="NBI42_002790"
     CDS             112..483
                     /locus_tag="NBI42_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCD1779119.1"
                     /translation="MLKKWLGMALITPMLTFIIWFFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
BASE COUNT          172 a           60 c           69 g          189 t
ORIGIN      
        1 atgattgatt ttagaatttt aataaaactg aagaaattaa aaaatcattc ctcaatctct
       61 cattttaatg taaactatgc gtaagagttt tctaaaagtg ggagtgatca aatgttgaaa
      121 aaatggcttg gaatggcact aataacccca atgttaacgt ttattatatg gttttttaat
      181 agccatacta tcattacata tttaaatata ttgttttatg tttcattaat tatttttatt
      241 agcatatttt tgattttact tgtacaagaa ggcatttttg atgcgacaag ttatggtttt
      301 agaagattaa agtatcaaat gtcatcttcg aaaaagaaga agtctatctc tgatgatcca
      361 tttttcaacc cacaagaggt aaaaaaagag cattactttg tttctacatg gataataccc
      421 ttattattaa taaatatttt atattttatt atgacgattg tgctgtcgct catcttagta
      481 taaagaaact
//
LOCUS       DAHHRR010000310          489 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.317, whole genome shotgun sequence.
ACCESSION   DAHHRR010000310 DAHHRR010000000
VERSION     DAHHRR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.317"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>489
                     /locus_tag="NBI42_002791"
     CDS             <1..>489
                     /locus_tag="NBI42_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1779120.1"
                     /translation="HFKNLKQVINELDIEQEKLIFLNVENHKLLNNKEQDLSNSAPLI
                     YKTLSALYHNFDGFGLNIFDNHQAFNAMHLYDKNGFKTTLGLILEKFIEYVSKPKYEN
                     SYYSIFDIENYYCLVIYDWRVIESETIMSNFEDSQVYINFKNNVLNDKYLIVIETLDE
                     NSG"
BASE COUNT          195 a           48 c           79 g          167 t
ORIGIN      
        1 gcattttaaa aatttaaaac aagtaattaa tgaattggat attgaacaag aaaagctgat
       61 ttttctaaat gttgaaaatc ataaactgct taataataaa gaacaagatt taagtaatag
      121 tgctccatta atttataaga cattaagtgc gctgtatcac aactttgatg ggtttggatt
      181 gaacattttt gacaatcatc aagcatttaa tgcgatgcat ctatatgaca aaaatggatt
      241 taaaacaaca ctaggtctta tattggaaaa atttatcgaa tatgtctcga aaccaaaata
      301 tgaaaacagt tattattcta tttttgatat agagaattat tattgtcttg ttatttatga
      361 ttggcgagtg atagagagcg agacaattat gagtaatttt gaggatagtc aagtttatat
      421 aaattttaaa aacaatgttt taaacgataa atatctaatt gtaatagaaa cattggacga
      481 aaatagtgg
//
LOCUS       DAHHRR010000311          479 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.318, whole genome shotgun sequence.
ACCESSION   DAHHRR010000311 DAHHRR010000000
VERSION     DAHHRR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.318"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..>479)
                     /locus_tag="NBI42_002792"
     CDS             complement(<1..>479)
                     /locus_tag="NBI42_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD1779121.1"
                     /translation="HQEYWYLYTGIMLIAGISYVFYQRDIESKRLLTSIGVGIITAII
                     LIMLQLLFSLITSNLSYGSLIKELARTGVNWKWQMLVTLLFVIPCHELYMRTVLQKEL
                     THFSIPKWVAILITAICSSSLFIYLDNWWIVTFIFVAQVILSLSYEYTRRIATTSVA"
BASE COUNT          182 a           76 c           62 g          159 t
ORIGIN      
        1 tgcaacggat gttgttgcta tgcgtcgagt atactcataa cttaatgata atatcacttg
       61 tgcaacaaat ataaatgtta ctatccacca attatctaaa taaataaata atgaacttga
      121 acaaattgca gttattaata tagctaccca tttcggtatt gaaaaatgtg ttaattcttt
      181 ttgtaaaaca gttctcatat ataactcgtg acatggaatg acaaaaagta aagttactaa
      241 catttgccac ttccaattga cacctgttct tgctaattct ttaattaatg aaccataact
      301 taaattagaa gttataagtg agaataaaag ttgaagcata attaaaataa ttgccgtaat
      361 aataccaaca ccaattgatg taagcaaccg cttagattca atatctcttt gataaaatac
      421 ataactgata ccagcaatta gcattatacc tgtatataaa taccagtatt cttggtgtt
//
LOCUS       DAHHRR010000312          477 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.319, whole genome shotgun sequence.
ACCESSION   DAHHRR010000312 DAHHRR010000000
VERSION     DAHHRR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.319"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..444)
                     /locus_tag="NBI42_002793"
     CDS             complement(<1..444)
                     /locus_tag="NBI42_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCD1779122.1"
                     /translation="MEIKEIVANIKRPYLTPFVIFTILLSLFFDAIMFFNSKLYDKLP
                     LYLVVFLIFAIVVSILLYLQEKSEKVKVEKKNVIWYMTLNVITGYSMPLFIASTYVVG
                     AAVNDIDVFYYWCGVALMLFISWLGLFLFYKNEFDSENPNPAVNFI"
BASE COUNT          195 a           89 c           42 g          151 t
ORIGIN      
        1 tataaagtta actgcaggat taggattttc actatcaaat tcatttttat aaaataaaaa
       61 taaacctaac caagaaataa ataacatcaa agctactccg caccaataat aaaacacatc
      121 tatatcgttt actgctgcac caacaacata agtagaagca ataaataatg gcattgaata
      181 accagttata acatttagtg tcatatacca aatcacattc tttttctcaa cttttacctt
      241 ctcacttttt tcttgtaaat acagtaaaat tgacactact attgcaaaaa ttaaaaatac
      301 gactaaatat aatggcaact tatcataaag tttactattg aaaaacataa tcgcatcaaa
      361 aaacaatgag agcaaaatcg taaatattac aaaaggtgtt aagtacggcc tttttatatt
      421 tgctacaatt tcctttatct ccattacact tatcccccct atacaaaaat ctttaat
//
LOCUS       DAHHRR010000313          451 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.320, whole genome shotgun sequence.
ACCESSION   DAHHRR010000313 DAHHRR010000000
VERSION     DAHHRR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.320"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
BASE COUNT          122 a          102 c           75 g          152 t
ORIGIN      
        1 aaatactaat ttctatagaa aaagtatttc tttagcgtcg tcccacccca acttgcattg
       61 cctgtagaaa ttgtagattc aatttctctg tgttgggccc atccccaact tgcacattat
      121 tgtaagctga cttttcgtca acttctgtgt tggggccccg ccccaactcg cattgcctgt
      181 agaatttctt ttcgaaattc tctgtgttgg ggcccacacc ccaacttgca ttgcctgtag
      241 aatttctttt cgaaattctc tgtgttgggg cccctgacta gaattgaaaa aagcttgtta
      301 caagcgcatt ttcgttcaat caactactgc caatataact tcgtagatca tagaacattg
      361 atttatttcc cagcctgttt gaacttgcaa gtaacatttc ataatgtatg aattcaaaag
      421 gagcatgtaa acaatataat ttttaagact c
//
LOCUS       DAHHRR010000314          450 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.321, whole genome shotgun sequence.
ACCESSION   DAHHRR010000314 DAHHRR010000000
VERSION     DAHHRR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.321"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..182
                     /locus_tag="NBI42_002794"
     CDS             <1..182
                     /locus_tag="NBI42_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779123.1"
                     /translation="EYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDNLT
                     LNTTEKLYEYANKVL"
BASE COUNT          188 a           50 c           68 g          144 t
ORIGIN      
        1 cagaatacaa gaaaaagata attgaattaa ttgaaagtaa tttaacagga tatgaaattt
       61 ctaaaaaaac tggagtttct caatatgtac tttcacaatt aagacagggc aaacgcgaag
      121 tagataatct aaccctgaat acaacagaaa aattatatga atatgccaat aaagttttgt
      181 aatataacta ctgtataaat taatcaagct atgtttattt gatttaacta ttaatgaaaa
      241 tcatatggta aatggatatt ataataatta aaatacaaaa atagtagatt ccaatttgtg
      301 aaaaaggagt atgtcgtttc atttagaatt tattgcacga aattagcatc gaataagggg
      361 aaataagagt taagttttaa atgataaaat agattggaac ggatcgcctt gaaatgctcc
      421 cttcaaagtt ttcatttttt caatgtctac
//
LOCUS       DAHHRR010000315          444 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.322, whole genome shotgun sequence.
ACCESSION   DAHHRR010000315 DAHHRR010000000
VERSION     DAHHRR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.322"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            138..>444
                     /locus_tag="NBI42_002795"
     CDS             138..>444
                     /locus_tag="NBI42_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCD1779124.1"
                     /translation="MKKEILKKQWVIWTILVTSFFSIGTPGIAIIPFSLSIYALSKLI
                     LVNKFVEPDLVTLQNLKEKNKSINIENQKIKREIQELKNLKKDLISSIEEGTKELEHI
                     "
BASE COUNT          205 a           54 c           59 g          126 t
ORIGIN      
        1 aaattatata gtaaaagcga tagtagatta ttaaaaaata tatatagcat ttgttctcac
       61 tgcgtcaaaa ctctaaagca attaagccat cgagagtatt taaatacaca taaattcaaa
      121 ttaaggagaa taggtaaatg aaaaaagaaa ttttaaaaaa gcaatgggta atatggacaa
      181 tcttagtaac atctttcttt agtataggca caccaggtat agcaataatc ccgttcagtt
      241 tatcaatata tgcattatct aaattaatac ttgttaataa atttgtagaa cccgatttag
      301 taacacttca aaacttgaaa gaaaaaaata aaagtatcaa cattgaaaat caaaaaatca
      361 agcgtgaaat acaagaattg aaaaatctaa aaaaagattt aatatctagt atagaagaag
      421 gtactaaaga acttgaacac atta
//
LOCUS       DAHHRR010000316          435 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.323, whole genome shotgun sequence.
ACCESSION   DAHHRR010000316 DAHHRR010000000
VERSION     DAHHRR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.323"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(50..>435)
                     /gene="icaR"
                     /locus_tag="NBI42_002796"
     CDS             complement(50..>435)
                     /gene="icaR"
                     /locus_tag="NBI42_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCD1779125.1"
                     /translation="NNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNTP
                     EEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMSKEDFQNLILLFLESWYLKASFSQK
                     FGAVEESKSQFKDEVYSLLNIFLKK"
BASE COUNT          151 a           65 c           45 g          174 t
ORIGIN      
        1 gtcttttttt tacaaaagta agttaattat tacaaactag taacaaaaat tatttcttca
       61 aaaatatatt tagtagcgaa tacacttcat ctttgaattg acttttactt tcttccactg
      121 ctccaaattt ttgcgaaaag gatgctttca aataccaact ttcaagaaat agcaatatta
      181 aattctgaaa gtcttctttt gacatcttta tctttgattc atcatagaat tttgctatct
      241 ctttacttaa tgattgattt aaatcttgta tttgtccgta aatatttcca gaaaattcct
      301 caggcgtatt agataattga acgtacattc ttatatacct ttcttcgatg tcgaaaataa
      361 actcaaataa gaattgatat aaagcatcaa ttgaatagtt cgatttattt tgattcatca
      421 taataatatt attaa
//
LOCUS       DAHHRR010000317          431 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.324, whole genome shotgun sequence.
ACCESSION   DAHHRR010000317 DAHHRR010000000
VERSION     DAHHRR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.324"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..>431)
                     /locus_tag="NBI42_002797"
     CDS             complement(<1..>431)
                     /locus_tag="NBI42_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779126.1"
                     /translation="LDHISKGIAYIACVLIPLNFFFRFNDIKKDNNYNNIISTLFLLL
                     YFLVNGISLIIQGFTAEFTISLISKSNIHSNHEFAVNLFRYVIQEGGISFSTYLVCNF
                     CIIMWLFFSCSLLKERKPVVRCIPLIISCLKLILILLFLLS"
BASE COUNT          178 a           54 c           51 g          148 t
ORIGIN      
        1 attgaaagta aaaatagtag aattaatatt aattttaaac aagatataat taatggtata
       61 catctgacaa ctggttttcg ttcttttaat aaagaacacg aaaagaataa ccacattatt
      121 atgcaaaagt tacacactag atatgtacta aaagaaatac caccttcttg tatgacatat
      181 ctaaataagt tcacagcaaa ttcatgatta ctatgaatat ttgacttact tattaaacta
      241 attgtaaatt ctgcagtgaa tccttgaata atcaatgaaa ttccatttac tagaaagtat
      301 aatagtagaa ataacgtact tataatatta ttatagttat tatctttttt gatgtcattg
      361 aatctaaaaa agaaattaag tggaattaac acacaggcaa tgtaagctat tccttttgat
      421 atatgatcca a
//
LOCUS       DAHHRR010000318          429 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.325, whole genome shotgun sequence.
ACCESSION   DAHHRR010000318 DAHHRR010000000
VERSION     DAHHRR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.325"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..260
                     /locus_tag="NBI42_002798"
     CDS             <1..260
                     /locus_tag="NBI42_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779127.1"
                     /translation="FIVRLLFRKKNVDLTEVVVLEHLIQWFLVYFAIYQAVNEKMDIN
                     DINIDNFQSVFFDVSNLNLVILPTLIISWVTIFNYRMRSYK"
BASE COUNT          146 a           49 c           68 g          166 t
ORIGIN      
        1 tatttatagt tagattactt tttagaaaaa agaatgtcga tttgacagag gttgtcgttt
       61 tagaacattt aattcaatgg ttcttagttt actttgcgat ctatcaggca gtaaatgaaa
      121 aaatggacat taatgatatt aatatcgaca atttccaatc tgtctttttt gacgtgtcta
      181 atttgaattt agtaattcta ccaacgttaa tcattagctg ggtcacaata tttaactata
      241 gaatgagaag ttacaaataa aatctatgaa attataccat cagacaccaa cattcatatg
      301 gtgtcttttt tgttgtgtat ttgtattttt gaaattctaa aaagtagagg tatgaatctt
      361 ttgactagtg tataagtact gatgagtcac aagatagata actatatttt gtctatatta
      421 taaagtgtt
//
LOCUS       DAHHRR010000319          425 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.326, whole genome shotgun sequence.
ACCESSION   DAHHRR010000319 DAHHRR010000000
VERSION     DAHHRR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.326"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>425
                     /locus_tag="NBI42_002799"
     rRNA            <1..>425
                     /locus_tag="NBI42_002799"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          136 a           79 c          116 g           94 t
ORIGIN      
        1 taagggcgca cggtggatgc cttggcacta gaagccgatg aaggacgtta ctaacgacga
       61 tatgctttgg ggagctgtaa gtaagctttg atccagagat ttccgaatgg ggaaacccag
      121 catgagttat gtcatgttat cgatatgtga atacatagca tatcagaagg cacacccgga
      181 gaactgaaac atcttagtac ccggaggaag agaaagaaaa ttcgattccc ttagtagcgg
      241 cgagcgaaac gggaagagcc caaaccaaca agcttgcttg ttggggttgt aggacactct
      301 atacggagtt acaaaggacg acattagacg aatcatctgg aaagatgaat caaagaaggt
      361 aataatcctg tagtcgaaaa tgttgtctct cttgagtgga tcctgagtac gacggagcac
      421 gtgaa
//
LOCUS       DAHHRR010000320          398 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.327, whole genome shotgun sequence.
ACCESSION   DAHHRR010000320 DAHHRR010000000
VERSION     DAHHRR010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.327"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            238..>398
                     /locus_tag="NBI42_002800"
     CDS             238..>398
                     /locus_tag="NBI42_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HCD1779128.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQ"
BASE COUNT          143 a           70 c           65 g          120 t
ORIGIN      
        1 attgaagcgg cttaacgatt aagtgtaaat tggtgcgtat accttacaaa aaggtgtatc
       61 aatcgacatt atcgttaagc tgtttttatg tgcgtttcat gaatccttcc tcaatcttcg
      121 tgaatattaa aattccatcg ccaacatcaa aattctcaac atcgcaacat caccaaatgt
      181 tataataaat ctattacaca aagagataaa ttacttattc aaaggcggag gaatcacatg
      241 tctattactg aaaaacaacg tcagcaacaa gctgaattac ataaaaaatt atggtcgatt
      301 gcgaatgatt taagaggaaa catggatgcg agtgaattcc gtaattacat tttaggcttg
      361 attttctatc gcttcttatc tgaaaaagcc gaacaaga
//
LOCUS       DAHHRR010000321          389 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.328, whole genome shotgun sequence.
ACCESSION   DAHHRR010000321 DAHHRR010000000
VERSION     DAHHRR010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.328"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..195)
                     /locus_tag="NBI42_002801"
     CDS             complement(<1..195)
                     /locus_tag="NBI42_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779129.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIAQFITIDFG"
BASE COUNT          152 a           60 c           48 g          129 t
ORIGIN      
        1 accgaaatca atggtaataa attgagctat tccataaatg atgacagcag agatagctaa
       61 cgaaatatat cctatgaata tgaataccac acccataaag agtgttttta agaacagaaa
      121 aggcttcttt ctatttgtaa ccaaatagct caatatacaa agtagtaata gaattgttaa
      181 aattttaatt atcatcgttt gacctcaata tatttattta tatctttatt attgaccacg
      241 aaaaaataac aatcacttaa gtgattgcca ataatactca atgcctactc tatagtaatc
      301 ttggcacgtg attttacact attttatcta tttaaaaatg taagtgttga attatgctta
      361 cttcgaaaat gcacaataca actaaatag
//
LOCUS       DAHHRR010000322          389 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.329, whole genome shotgun sequence.
ACCESSION   DAHHRR010000322 DAHHRR010000000
VERSION     DAHHRR010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.329"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..328)
                     /locus_tag="NBI42_002802"
     CDS             complement(<1..328)
                     /locus_tag="NBI42_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1779130.1"
                     /translation="MKHHAYKKVNTLLDVESYVFFLGRELKQNFELSTKEFLIIAYLY
                     YRKNEDVPLKEIIGDIFYKQSDVVKIVKFLNKKGFINKYRKSTDERCILISINEVQRT
                     KIYSMLI"
BASE COUNT          136 a           62 c           37 g          154 t
ORIGIN      
        1 caattagcat actataaatt ttagtccttt gcacttcgtt aattgatatt aaaatgcaac
       61 gttcatctgt actttttcta tatttattta taaatccctt tttattaaga aatttcacaa
      121 ttttaacaac atctgattgt ttataaaaaa tgtctcctat aatctccttt agaggtacat
      181 cctcattctt cctataataa agatatgcaa taattaaaaa ttcttttgtg gacaattcga
      241 aattttgttt tagttctcga cctagaaaaa aaacatagga ctctacatct aaaagggtgt
      301 taactttttt atatgcatga tgtttcatca taaattctcc tgacaccaat attctcacaa
      361 ctatttatag tatttaaagt aatataaaa
//
LOCUS       DAHHRR010000323          388 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.330, whole genome shotgun sequence.
ACCESSION   DAHHRR010000323 DAHHRR010000000
VERSION     DAHHRR010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.330"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(25..258)
                     /locus_tag="NBI42_002803"
     CDS             complement(25..258)
                     /locus_tag="NBI42_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779131.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT          136 a           66 c           36 g          150 t
ORIGIN      
        1 atgattctaa atgcttttta atatttaatg ctcatcaaca tttggatttt gaatattcaa
       61 ttcaaaaact ttattagcta cgtcaattgt aaaatcagaa ccatagttga catgagctac
      121 ttttaatttt ccatctaaat aatagattgc gattgcaaca tcgtaaaatt cgtcaatgac
      181 aaataaactc ttttcatttg ttacaacttc atgctctcct gagtatacaa cgttaatttc
      241 ccaatcatta aaaaccattt gttaacctcc ttgaacattt aaattgattc aacttaagtt
      301 taacttattc atacaacttc gtacaatatc tagatgaaca ttaattgtat ttctagaaat
      361 ctttttcaat tatatgtact aattatac
//
LOCUS       DAHHRR010000324          366 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.331, whole genome shotgun sequence.
ACCESSION   DAHHRR010000324 DAHHRR010000000
VERSION     DAHHRR010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.331"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>366
                     /locus_tag="NBI42_002804"
     rRNA            <1..>366
                     /locus_tag="NBI42_002804"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           99 a           66 c          112 g           89 t
ORIGIN      
        1 taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattt gatgcaaggt
       61 taagcagtaa atgtggagcc gtagcgaaag cgagtctgaa tagggcgttt agtatttggt
      121 cgtagacccg aaaccaggtg atctaccctt ggtcaggttg aagttcaggt aacactgaat
      181 ggaggaccga accgacttac gttgaaaagt gagcggatga actgagggta gcggagaaat
      241 tccaatcgaa cctggagata gctggttctc tccgaaatag ctttagggct agcctcaagt
      301 gatgattatt ggaggtagag cactgtttgg acgaggggcc cctctcgggt taccgaattc
      361 agacaa
//
LOCUS       DAHHRR010000325          361 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.333, whole genome shotgun sequence.
ACCESSION   DAHHRR010000325 DAHHRR010000000
VERSION     DAHHRR010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.333"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..258
                     /locus_tag="NBI42_002805"
     CDS             <1..258
                     /locus_tag="NBI42_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCD1779132.1"
                     /translation="KDNYKRITLIEESIKKYRYVKDQIKRFKNGLEESLLNATLSIEQ
                     NMLKVKIDYYKAYCFPREKTKHNNIEYNVSLFFKALDALKK"
BASE COUNT          160 a           34 c           51 g          116 t
ORIGIN      
        1 aaagataatt acaaaagaat tacactgata gaagaatcta ttaaaaagta tagatacgta
       61 aaagatcaga ttaaaagatt taaaaatgga ttagaagaat cgttattaaa tgcaacttta
      121 tctattgaac aaaatatgtt gaaagttaaa attgattatt ataaagccta ctgctttcct
      181 agagaaaaaa caaagcataa caatattgag tacaatgtat cgttgttttt taaagcatta
      241 gatgccttaa agaaatagtt tttcactgaa aatatttata gatataatta aaattaatta
      301 atattaagcc aggataatgt agtcttaatc gttctgaaat acgaaaaatg ttgtggaaat
      361 c
//
LOCUS       DAHHRR010000326          355 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.334, whole genome shotgun sequence.
ACCESSION   DAHHRR010000326 DAHHRR010000000
VERSION     DAHHRR010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.334"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>355
                     /locus_tag="NBI42_002806"
     rRNA            <1..>355
                     /locus_tag="NBI42_002806"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           95 a           82 c          105 g           73 t
ORIGIN      
        1 agacggtctt gctgtcactt atagatggat ccgcgctgca ttagctagtt ggtaaggtaa
       61 cggcttacca aggcaacgat gcatagccga cctgagaggg tgatcggcca cactggaact
      121 gagacacggt ccagactcct acgggaggca gcagtaggga atcttccgca atgggcgaaa
      181 gcctgacgga gcaacgccgc gtgagtgatg aaggtcttcg gatcgtaaaa ctctgttatt
      241 agggaagaac atatgtgtaa gtaactgtgc acatcttgac ggtacctaat cagaaagcca
      301 cggctaacta cgtgccagca gccgcggtaa tacgtaggtg gcaagcgtta tccgg
//
LOCUS       DAHHRR010000327          330 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.335, whole genome shotgun sequence.
ACCESSION   DAHHRR010000327 DAHHRR010000000
VERSION     DAHHRR010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.335"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..174
                     /locus_tag="NBI42_002807"
     CDS             <1..174
                     /locus_tag="NBI42_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1779133.1"
                     /translation="LLNDKSISNYRINQDTCVSYGGISELRSGKRKVENLTLETAEKL
                     YNYQKHLEIMIED"
BASE COUNT          152 a           25 c           49 g          104 t
ORIGIN      
        1 ttactaaatg acaaatcaat atctaattat agaattaatc aagatacttg tgtttcatat
       61 ggtggtataa gtgaattaag aagcgggaaa agaaaagtgg aaaatttaac tttagaaaca
      121 gcggaaaaac tttataatta ccaaaaacat ttagaaataa tgattgaaga ttaaaataag
      181 gacatatctt gtgatatata atggagtgag cttttaaatc atctaatttt aaagtatatt
      241 aaagatggat aactaaatct gaatataaat aaaaagtgat caaataatat aatatagaat
      301 agattgagac aagaaatttt atcattatat
//
LOCUS       DAHHRR010000328          326 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.337, whole genome shotgun sequence.
ACCESSION   DAHHRR010000328 DAHHRR010000000
VERSION     DAHHRR010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.337"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            73..155
                     /locus_tag="NBI42_002808"
     tRNA            73..155
                     /locus_tag="NBI42_002808"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:106..108,aa:Leu,seq:gag)
     gene            177..250
                     /locus_tag="NBI42_002809"
     tRNA            177..250
                     /locus_tag="NBI42_002809"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:209..211,aa:Gly,seq:tcc)
BASE COUNT          104 a           62 c           68 g           92 t
ORIGIN      
        1 tgacaaatat ttttaatcat aatataataa ataacgttct gaaaaacgga gatgttttaa
       61 atgcctatat ccgcggtcgt ggcggaatgg cagacgcgct aggttgaggg cctagtggga
      121 gaaatcccgt ggaggttcaa gtcctctcgg ccgcatcaaa attcttaatt aaataagcgg
      181 gtgtagttta atggcaaaac ctcagccttc caagctgatg ttgtgggttc gattcccatc
      241 acccgctcca tagataattt taatgaacat tgaaaactga atgacaatat gtcaacgtta
      301 attccaaaaa cgtaactatt agttac
//
LOCUS       DAHHRR010000329          316 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.338, whole genome shotgun sequence.
ACCESSION   DAHHRR010000329 DAHHRR010000000
VERSION     DAHHRR010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.338"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(55..>316)
                     /locus_tag="NBI42_002810"
     rRNA            complement(55..>316)
                     /locus_tag="NBI42_002810"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           78 a           83 c           71 g           84 t
ORIGIN      
        1 atatgtcacg ttattccgca tcttctgaag aagatgttcc gaatatatcc ttagaaagga
       61 ggtgatccag ccgcaccttc cgatacggct accttgttac gacttcaccc caatcatttg
      121 tcccaccttc gacggctagc tcctaaaagg ttactccacc ggcttcgggt gttacaaact
      181 ctcgtggtgt gacgggcggt gtgtacaaga cccgggaacg tattcaccgt agcatgctga
      241 tctacgatta ctagcgattc cagcttcatg tagtcgagtt gcagactaca atccgaactg
      301 agaacaactt tatggg
//
LOCUS       DAHHRR010000330          315 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.339, whole genome shotgun sequence.
ACCESSION   DAHHRR010000330 DAHHRR010000000
VERSION     DAHHRR010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.339"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
BASE COUNT          132 a           56 c           24 g          103 t
ORIGIN      
        1 attattaaca aatatctcca aaaacccatt aaaattataa gtcactttat gatttaaatc
       61 tttactagga aaatcactaa agtcctgtat attcatacca attatgtaaa atactattat
      121 tagaatcaaa caaataaaac aattgaattg cataaatttg ttactgcgtt tcaaaaatga
      181 agataaactc aagtcattca ttgattacgc ccccatctat aattactatt tcactaaata
      241 gatacgttca ctcgcaaacg atatcatcct taaaaatcga ttaaaaaaca gctatattaa
      301 aacctttaat ttacc
//
LOCUS       DAHHRR010000331          303 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.341, whole genome shotgun sequence.
ACCESSION   DAHHRR010000331 DAHHRR010000000
VERSION     DAHHRR010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.341"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>303
                     /locus_tag="NBI42_002811"
     CDS             <1..>303
                     /locus_tag="NBI42_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein"
                     /protein_id="HCD1779134.1"
                     /translation="PSEPETPTPPTPEVPSEPGEPTPPTPEVPSEPGTPVPPAKEEPK
                     KPSKPVEQGKVVTPVIEINEKVKAVAPTKQTQSKKSELPETGGEESTNKGMLFGGLF"
BASE COUNT          121 a           76 c           63 g           43 t
ORIGIN      
        1 ccaagtgagc cggaaacacc aacaccaccg acaccggaag taccgagtga gccaggcgaa
       61 ccaacgccac cgacaccaga agtaccaagt gagccaggca caccagtacc acccgcaaaa
      121 gaagaaccta aaaagccttc taaaccagtg gaacaaggta aagtagtaac acctgttatt
      181 gaaatcaatg aaaaggttaa agcagtggca ccaactaaac aaacacaatc taagaaatct
      241 gaactacctg aaacaggtgg agaagaatca acaaacaaag gtatgttgtt cggcggatta
      301 ttc
//
LOCUS       DAHHRR010000332          291 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.342, whole genome shotgun sequence.
ACCESSION   DAHHRR010000332 DAHHRR010000000
VERSION     DAHHRR010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.342"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..>291)
                     /locus_tag="NBI42_002812"
     CDS             complement(<1..>291)
                     /locus_tag="NBI42_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1779135.1"
                     /translation="LHDSIRMMWLAQDQTPSYKTINRFRVNPNTDALIESLFIQFHSQ
                     CLKQNLIDDKSIFIDGTKVEASANRYTFVWKKSIQNHESKLNENSKALYRDLV"
BASE COUNT           97 a           46 c           40 g          108 t
ORIGIN      
        1 actaagtcac gatataatgc ttttgaattt tcgttcaatt tcgattcgtg attttgaata
       61 cttttcttcc acacaaatgt atatctattg gcactagctt ctacttttgt accatcaata
      121 aaaattgatt tatcatcaat aagattttgc tttaaacatt gactatggaa ctgaataaat
      181 aaagattcaa ttagcgcatc agtattagga ttcactctaa aacgattaat agttttataa
      241 gaaggtgttt gatcttgagc taaccacatc attcgaatac tgtcatgaag t
//
LOCUS       DAHHRR010000333          289 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.343, whole genome shotgun sequence.
ACCESSION   DAHHRR010000333 DAHHRR010000000
VERSION     DAHHRR010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.343"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>289
                     /locus_tag="NBI42_002813"
     CDS             <1..>289
                     /locus_tag="NBI42_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1779136.1"
                     /translation="NEIVETIPDSEFDEFRHHRGATSYHPKMMLKIILYAYTQSVFSG
                     RRIEKLLHDSIRMMWLAQNQTPSYKTINRFRVNPNTDALIESLFIQFHSQC"
BASE COUNT          100 a           47 c           41 g          101 t
ORIGIN      
        1 ttaatgaaat tgttgaaacg atacctgata gcgaattcga tgaattcaga catcatcgtg
       61 gcgcaacatc ctatcatcca aaaatgatgt taaaaattat cttatatgca tacactcaat
      121 ctgtattttc tggtcgtaga atagagaaat tacttcatga cagtattcga atgatgtggt
      181 tagctcaaaa tcaaacacct tcttataaaa ctattaatcg ttttagagta aatcctaata
      241 ctgatgcatt aattgaatct ttatttatcc agtttcatag tcaatgttt
//
LOCUS       DAHHRR010000334          240 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.347, whole genome shotgun sequence.
ACCESSION   DAHHRR010000334 DAHHRR010000000
VERSION     DAHHRR010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.347"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(11..214)
                     /locus_tag="NBI42_002814"
     CDS             complement(11..214)
                     /locus_tag="NBI42_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCD1779137.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     ISNYTDHQLIETTNRAISLYMAN"
BASE COUNT           74 a           41 c           35 g           90 t
ORIGIN      
        1 actactcaaa ttaatttgcc atatataagc taatagctct atttgtagtt tcaattaatt
       61 gatgatctgt gtaattagat atcacatatg ctctaattac ttggtgtctt tcttctaatc
      121 gagggaatgc tttatcttca ctaacgtgtt cggccaatat accaaatgga gtgttatcat
      181 tttgaaaacc catgatgaaa ttgtaaaacg tcataagatt tgcccccttc atttattaag
//
LOCUS       DAHHRR010000335          222 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.349, whole genome shotgun sequence.
ACCESSION   DAHHRR010000335 DAHHRR010000000
VERSION     DAHHRR010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.349"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..>222)
                     /locus_tag="NBI42_002815"
     rRNA            complement(<1..>222)
                     /locus_tag="NBI42_002815"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           76 c           43 g           55 t
ORIGIN      
        1 gtggtacagg aatatcaacc tgttatccat cgcctacgcc tgtcggcctc agcttaggac
       61 ccgactaacc cagagcggac gagccttcct ctggaaacct tagtcaatcg gtggacggga
      121 ttctcacccg tctttcgcta ctcacaccgg cattctcact tctaagcgct ccacatgtcc
      181 ttacgatcat gcttcaacgc ccttagaacg ctctcctacc at
//
LOCUS       DAHHRR010000336          220 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.350, whole genome shotgun sequence.
ACCESSION   DAHHRR010000336 DAHHRR010000000
VERSION     DAHHRR010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.350"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..>220
                     /locus_tag="NBI42_002816"
     CDS             <1..>220
                     /locus_tag="NBI42_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCD1779138.1"
                     /translation="IKEDGDSDLTIEEIDLIGSHLDKEIEDLNHSIQNEDCTQIRKQT
                     RKKRTEIKKFKKKFDDYSERKSKYEEQKS"
BASE COUNT          109 a           20 c           38 g           53 t
ORIGIN      
        1 ataaaagaag atggagatag cgatttaaca atagaagaaa tagatttaat tggtagtcat
       61 ttagataaag aaatcgaaga tttaaatcat tctattcaga acgaagattg tactcaaatt
      121 agaaaacaga cccgtaaaaa aagaactgag attaagaagt tcaaaaagaa atttgatgat
      181 tattccgaaa gaaaaagtaa atatgaagaa caaaaatcga
//
LOCUS       DAHHRR010000337          219 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.denovo.351, whole genome shotgun sequence.
ACCESSION   DAHHRR010000337 DAHHRR010000000
VERSION     DAHHRR010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.denovo.351"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
BASE COUNT           78 a           32 c           27 g           82 t
ORIGIN      
        1 acgataaacg tacgggtcca ctactaaagg gagtcaaatt tttaccttcg tttgtatcat
       61 gcagcgtttt acacatactt ttaagagatg tttattcgtt atcgaaggta cacctttatt
      121 ataacttata tcatttttat taaaatacaa ttcaaaaaaa gttattgaca tattattgtc
      181 taaataatgt ataacctttg aacaacaata atgattatt
//
LOCUS       DAHHRR010000338         6151 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.guided.132, whole genome shotgun sequence.
ACCESSION   DAHHRR010000338 DAHHRR010000000
VERSION     DAHHRR010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.guided.132"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..1313
                     /locus_tag="NBI42_002817"
     CDS             <1..1313
                     /locus_tag="NBI42_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCD1779139.1"
                     /translation="LFNHQYKKNIVIRKAEAIQSPITFWYGKYIILIPSSYFKSVIDK
                     RLKYIILHEYAHAKNRDTLHLIIFNIFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLN
                     NINKNEFKTYAESIMDSVLKTSFFNKNILSHSFNGKKSLLKNRLINIKEADLKKQSKL
                     ILIFICIFTFFIMIIQSQFLMGQSLTDYNYKKPLQSDYQILDESKNFGSNSGSFVMYS
                     MKKDKYYIYNEKESRKRYSPDSTYKIYLALFGLDRHIISDKNSRMSWNHKHYLFESWN
                     KEQDLNTAMQNSVNWYFERISNQIPKNYTAAQLKQLNYGNENLGSYKSYWMEDSLKIS
                     NLEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWF
                     VGYVITNHDKYYFATHLSDGNPSGKNAELISEKILKGMGVLNGQ"
     gene            1303..1683
                     /gene="blaI"
                     /locus_tag="NBI42_002818"
     CDS             1303..1683
                     /gene="blaI"
                     /locus_tag="NBI42_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCD1779140.1"
                     /translation="MANKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            1947..2540
                     /locus_tag="NBI42_002819"
     CDS             1947..2540
                     /locus_tag="NBI42_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1779141.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
     gene            2512..3955
                     /locus_tag="NBI42_002820"
                     /pseudo
     CDS             2512..3955
                     /locus_tag="NBI42_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            3948..4763
                     /locus_tag="NBI42_002821"
     CDS             3948..4763
                     /locus_tag="NBI42_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1779142.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAII
                     DLGQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMI
                     FIGMPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDY
                     EAITTIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
     gene            complement(4883..5857)
                     /locus_tag="NBI42_002822"
     CDS             complement(4883..5857)
                     /locus_tag="NBI42_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCD1779143.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFN
                     SSSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFE
                     MNKASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENS
                     GWIRVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIE
                     DIENYLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYE
                     EDLKLLQKNYKTTLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
     gene            complement(6069..>6151)
                     /locus_tag="NBI42_002823"
     CDS             complement(6069..>6151)
                     /locus_tag="NBI42_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD1779144.1"
                     /translation="FVHIIAPVISGCAVAYYTYWLSKRNK"
BASE COUNT         2340 a          818 c          948 g         2045 t
ORIGIN      
        1 tacttttcaa ccatcaatat aaaaaaaata ttgtgattcg aaaagcagag gctattcaat
       61 ctccaataac tttttggtat gggaaatata ttattttgat tcctagttca tattttaaaa
      121 gtgtaattga taaaagacta aaatatataa tactgcatga atatgctcat gctaaaaata
      181 gagatacttt acatttaatt atctttaata tcttctctat tattatgagt tacaatccat
      241 tagtacatat tgtaaaaaga aagataattc atgacaatga agtagaagcc gatagatttg
      301 tccttaataa tattaacaaa aatgaattta aaacttatgc ggaatctatt atggactccg
      361 tattaaaaac ctcatttttc aacaaaaata tattaagcca ttcatttaat ggtaaaaaat
      421 cattactcaa aaatagatta attaatataa aagaagccga tctgaaaaag cagtcaaaat
      481 taattctaat ttttatttgt atttttactt tttttatcat gataattcaa agccagtttt
      541 taatggggca atccttaact gattataatt ataaaaagcc cttacaaagc gattaccaaa
      601 ttttagatga aagtaaaaat tttggttcaa atagtggatc tttcgttatg tacagcatga
      661 agaaagacaa atactatatt tataatgaaa aagaaagtag aaaaagatat tctcctgatt
      721 caacttacaa aatttattta gcactgtttg gacttgaccg tcatattata agtgataaaa
      781 attcacgtat gtcatggaat cataaacatt atcttttcga gtcttggaat aaggaacaag
      841 atttaaatac agcaatgcaa aattcagtta attggtactt cgaacgtatt agtaatcaaa
      901 taccaaagaa ctatactgcg gctcaactca agcaattaaa ttatggtaat gaaaatttgg
      961 gaagttataa aagctattgg atggaagata gtttgaaaat atctaatctt gaacaagtaa
     1021 tagtttttaa aaatatgatg gaacaaaata accattttag taaaaaagca aagaatcaat
     1081 tatcttcttc attattgatt aagaaaaatg aaaagtatga actgtatggg aaaacaggta
     1141 caggtatagt aaacgggaag tataataatg ggtggtttgt aggttacgta attacaaatc
     1201 atgataagta ttattttgct acacatttat cagatggaaa cccgtctggg aaaaatgctg
     1261 aattaataag tgaaaaaata ttaaaaggaa tgggtgtttt aaatggccaa taagcaagtt
     1321 gaaatatcta tggctgaatg ggatgttatg aatataatat gggataaaaa atcagtatca
     1381 gctaatgaaa ttgtagttga aattcaaaaa tataaagaag ttagtgataa aacgattaga
     1441 acattaatca caagactata taaaaaagag attataaaac gatacaaatc agaaaatatt
     1501 tatttttact catcaaatat taaagaagac gatattaaaa tgaaaactgc taaaaccttt
     1561 cttaataaac tgtatggagg ggatatgaaa agtttagtgt tgaattttgc gaaaaatgaa
     1621 gaattaaata acaaagaaat tgaagaattg cgagatattt taaatgatat tagtaaaaag
     1681 taattagagg tatgtttcaa acataataca tatgtaaact atgcttgact cttattaaat
     1741 tagttttgtt aatgacattt aaaatatatt attaaagatg agagtgtaca gaaaataacc
     1801 attttctgta cactcttctt ttatgtctaa ataagtaaga attacatagg attatgtgtt
     1861 caaaaaccca acaaaaaatc tatgttcata aaagaccttg taattaagtt atggtacatt
     1921 gaagatgtat ataggaggtt tgtattttga aaataggtta tgcaagagtt tcaactggat
     1981 tacaaaattt aaatttacag gaggatcgat taaatacata tggttgtgaa aagatattta
     2041 atgaccatat gagtggttca aaaagtaaaa gacctggttt agataaagca attgaatttg
     2101 cgaggtcagg agatacgatt gttgtttgga gattagatag attgggacgt aatatggagg
     2161 atttaatcac attagttaat gagcttaacg aacgaggtgt aagtttccat agtttagaag
     2221 aaaatataac aatggataaa tcaagttcaa ctggacaatt attatttcat ttattcgcag
     2281 catttgcaga atttgaacgt aatttaattt tagagcgttc ttcagctggc cgaattgcag
     2341 cacgtgctag aggccgatat ggaggcagac cagaaaaatt aaatcaaaaa gatttaaact
     2401 tacttaagac cctttatgat aatggcacgc caattaagac aattgcagaa cagtggcatg
     2461 tttcacgtac aacaatttat cgttacctca ataaattgga ggacaaggaa gatgaaaaac
     2521 aaggagaagt atctaactaa tttttctgaa gccaaacgta aaaaagctac tcaaaagtac
     2581 aatattataa aaccttttat cctaggcgaa caatctttat caagtatttc taaaagtaaa
     2641 gggattgcgt taagcaccct ttataggtgg aataagtcat ataaagaaca aggacttaaa
     2701 ggactaatct atgcgacaag agctgataaa ggaacacgta aaatagaacc aaagataatt
     2761 gatgaaattg aacgccttgc gcttatgaat aaaagaaata gtatagccac aattcatagg
     2821 aaaatcacta attactgtaa agaaaataat tttgatatac caagttataa acaaatttat
     2881 agtgttatta aagcgatgcc taagtctgtt attgatttct ctcatcaggg tgaaaaatac
     2941 tatcaaaata agtacgattt aatacaaatt agagaatcct cgagacccaa tgaaatatgg
     3001 caagcagacc atactttgct agatatcttt atattagacc aaaaaggtaa tatcaataga
     3061 ccatggctaa ctattattat ggatgactat agtcgtgcaa ttgcaggcta tttcttaagt
     3121 tttgatgccc cccaatgctc aaaacacagc attaacacta caccaagcaa tatggaataa
     3181 aaatgatacc aattggccag tgtgtggtat acccgaaaaa ttctacacag atcatggaag
     3241 tgactttact tcacatcata tggaacaagt cgctattgat ttgaaaatta acttgatgtt
     3301 ttcgaaagtt ggtgttcctc gtggtcgtgg aaaaatagaa cgattctttc agacagttaa
     3361 tcaaactttc ttagaacaac tgcctggata tataaacaat aatgatactc ctagtaatct
     3421 aataaatttt cacaattttg aagaaaaatt acgttacttt ttaattgaag attataatca
     3481 aaaagaacat agtactattc ataccacgcc tatcagtcat tggaatagta atcacttttt
     3541 ccctaatatg ccaagtagct tagaacaact tgatttatta ttgttggaaa tacctaaatc
     3601 tagaaaagtt cattcagatg gcattcattt tcaaggtttt cgatacagta atactaatct
     3661 agaagcttat gtaggtgaat atgtactgat tcgttataac ccaaatgata tggctgaaat
     3721 acgtgtgttt tatagagatg aatttctttg tactgctata tgtccagaaa tagctgatta
     3781 ctccatagat ataaaagaca tacagcacgc tcgtagtcag agaagaaaac acttaaaaca
     3841 aaatattgct agtccaagta ccacggaatt aattaaggaa gaaaaaaaat gtggttattc
     3901 acctcaagaa acgactaaaa acgtcaaaaa attaaagaga tatcgtaatg actaaaaatc
     3961 aaaatttcat tgaaacgaaa gagtataaac gctttgctga attttgtgat gcttgtatta
     4021 aatatcagta tattggtata tgttatggtc aacctggtgt cggaaaaact ttatcgtcta
     4081 ggtattacac aaattgggat actattgaaa aacaagttaa ccatagaggt tgggaagatt
     4141 tagctagtaa aacgaccgat gacatactat cggtggataa aatattttat aatgcaccag
     4201 cagaaaaaca aactaaatta aggaatgatt tatatagtat tactgcaatc attgatttgg
     4261 gtcaaaaatt acatgttgta aataagtatg gacatgaaca tagtaaacat tatagtgata
     4321 tgtttaaata tattgattta attatagtag atgagattga tcgccttaaa gtgcaacatt
     4381 tagagcaatt aagagctatt tatgatgaac ataatttagc aatgatattt attggtatgc
     4441 cgggtataga gaaaaaacta tctcgttatc ctcaactata ttcgcgtata ggttttgcgc
     4501 atgaatttga taatctaagt aaagatgaga cgcatcatat attagaatat aagtggcaag
     4561 acttaggatt tgatttaaaa ctcgaagact ttactgatta tgaggcaata acgacgatta
     4621 ttaaaattac aaaagggaat tttaggctga ttcatcgttt atttgcgcag atagacagaa
     4681 ttatggatat aaatggttta gataaaataa gtacagaagt tgtagaaaca gctagagata
     4741 gtttagttat aggtattcga taatagaaaa agcaacgcat tttaatatag tgcgttgctt
     4801 ttctcattta ttaggtaaag tcattctcat atatcgagta aagtgacaac aattatatat
     4861 gtatgattag ggtgtactta tatcattcat ataaatgttt atttataccc tcaataaaaa
     4921 tattatatgc ttttaagcac ctatcacatt ccttataaaa atgtttattc aatcctttaa
     4981 tatcgactag cgtagtctta taattttttt gtaacaattt taaatcctct tcgtaaaagc
     5041 ttaatttaaa atgaccggct tgagctaatg gcttttttcc ttgtttaatt aaaatcaata
     5101 tgttttttaa atgttttaaa aagtttttat aatcataaac ttttattttc atgaatgatt
     5161 tatgtggatt attttcataa tttagcaggt agttttctat atcttcaata taactatcat
     5221 caaatggtgt gtgctggaaa taaaaagttt ttacatacat gaatatttta taaagttctt
     5281 ctttcaaaaa atcatatgta tctaaatctc tttgtatagc atccaatttt tttaattttt
     5341 ttacattttt accacaatcc tgtactctta tccatccact attttcttta atttcactag
     5401 gtacactttg cattaaagtc tgagatactt tactttttaa ttccgaaata tcattaatat
     5461 ttggactaat tagtctattt tctttaactt ttgtcttaaa ctcatctaat tttttagact
     5521 tttccgcgga ttcggtagat gctttattca tctcaaaaac atatataggt ttacctaaag
     5581 atttagcaaa attgtactcc atttctgtat agctaatatt ttcttcacta tcaattgttc
     5641 cataactaga acctaacaat aatatcactg catctgattc attgatccat tgtgttatta
     5701 tttcccattg gcttttacta cttgagttaa aaagttccat acctgctgga atattattac
     5761 tgtttaaaac tgcttctact actgcttgac gctcttctat taaatctaaa taagttgatg
     5821 aaataaaaat ttgatattta attttagtat tactcatata tgctccttta ataaaaaatc
     5881 aatttacata aatatatcat atgataatat ccaaaaagac tacttaaaat attatctttt
     5941 tgcattcctc tactaaccag tgttataatc tagataacga aaatgattaa caattaatgc
     6001 accaatcccc acactactgc aatagtgtgg ggattttttc gatgctggct tataaagtcg
     6061 cctattaatt atttgttgcg tttactaagc caataagtat aatacgcaac agcacagcca
     6121 ctgatgactg gtgctatgat gtgaacgaaa a
//
LOCUS       DAHHRR010000339         4803 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.guided.152, whole genome shotgun sequence.
ACCESSION   DAHHRR010000339 DAHHRR010000000
VERSION     DAHHRR010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.guided.152"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            complement(<1..452)
                     /locus_tag="NBI42_002824"
     CDS             complement(<1..452)
                     /locus_tag="NBI42_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCD1779145.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTVL
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNIITTKPVQEFATDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTIL"
     gene            559..1404
                     /gene="blaZ"
                     /locus_tag="NBI42_002825"
     CDS             559..1404
                     /gene="blaZ"
                     /locus_tag="NBI42_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCD1779146.1"
                     /translation="MKKLIFLIVMALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            1773..2381
                     /locus_tag="NBI42_002826"
     CDS             1773..2381
                     /locus_tag="NBI42_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1779147.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRP
                     VLQEALNFLRMGDRFVVESIDCLGRNYDEVIHTVNYLKDKEVQLMITSLPMMNEVVGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRIIYHRVVEMLEEGQSISKIAKEVNITRQTIYRIKNDNDLI"
     gene            2795..3088
                     /locus_tag="NBI42_002827"
     CDS             2795..3088
                     /locus_tag="NBI42_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCD1779148.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK
                     HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
     gene            3163..>4803
                     /locus_tag="NBI42_002828"
     CDS             3163..>4803
                     /locus_tag="NBI42_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCD1779149.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNW
                     DKSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLT
                     PSDLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLV
                     PFIFLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFI
                     ANILIIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKG
                     YKFSIFMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNI
                     RNSEEKQILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNF
                     YKNINHDFKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSK
                     NVSVFSFDRTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLK
                     DNHLEEEISGITNYKDSVLNELNETKTKMIITIVTIIIN"
BASE COUNT         1844 a          583 c          720 g         1656 t
ORIGIN      
        1 aaaagtatcg tatctatttt atttttttca ttttcgttta gataaagtga ctgtttcttt
       61 aaatatttaa gatataataa ggctttcaaa aatttaaaac ttaaaataat aactaaaact
      121 atccaaataa ctgtgcagat attatcaatt gaatcccaat taaacttatg gatatctgtt
      181 gcgaactctt gaacaggttt ggtggtaatt atgttatggt tcaagtcgtg tgacttactt
      241 tcaactgtgg gcgcttgatt attcacatta ttaaatttta taaaagagaa tttaattggg
      301 ataaaaggaa ttaatccagc aagcacagtt agataccaaa ctttataatt taaactataa
      361 ttaaaatagc gttttaatat gtacctaaaa aatactaaca atagaaatat aaaacaaaag
      421 cttactacac tcattattag taatttagcc attttgacac cttctttcaa atatttataa
      481 taaacaattg acatcaatat tacaattgta atattattgt tttgtaaaaa ttacaactgt
      541 aatatcggag ggtttatttt gaaaaagtta atatttttaa ttgtaatggc tttagtttta
      601 agtgcatgta attcaaacag ttcacatgcc aaagagttaa atgatttaga aaaaaaatat
      661 aatgctcata ttggtgttta tgctttagat actaaaagtg gtaaggaagt aaaatttaat
      721 tcagataaga gatttgccta tgcttcgact tcaaaagcga taaatagtgc tattttgtta
      781 gaacaagtac cttataataa gttaaataaa aaagtacata ttaacaaaga tgatatagtt
      841 gcttattctc ctattttaga aaaatatgta ggaaaagaca tcactttaaa agaacttatt
      901 gaggcttcaa tgacatatag tgataataca gcaaacaata aaattataaa agaaatcggt
      961 ggaatcaaaa aagttaaaca acgtctaaaa gaactaggag ataaagtaac aaatccagtt
     1021 agatatgaga tagaattaaa ttactattcg ccaaagagca aaaaagatac ttcaacgcct
     1081 gctgctttcg gcaagacttt aaataaactt atcgcaaatg gaaaattaag caaaaaaaat
     1141 aaaaatttct tacttgattt aatgtttaat aataaaaacg gagacacttt aattaaagat
     1201 ggtgttccaa aagactataa ggttgctgat aaaagtggtc aagcaataac atatgcttct
     1261 agaaatgatg ttgcttttgt ttatcctaag ggccaatctg aacctattgt tttagtcatt
     1321 tttacgaata aagacaataa aagtgataag ccaaatgata agttgataag tgaaaccgcc
     1381 aagagtgtaa tgaaggaatt ttaatattct aaatgcataa taaatactga taacatctta
     1441 tagtttgtat tatattttct attatcgttg acatgtataa ttttgatatc aaaacctgat
     1501 tttcccttta ttattttcga gatttatttt cttaattctc tttaacaaac tagaaatatt
     1561 gtatatacaa aaaatcataa ataatagatg aatagtttaa ttataggtgt tcatcaatcg
     1621 aaaaagcaac gtatcttatt taaagtgcgt tgcttttttc tcatttataa ggttaaataa
     1681 ttctcatata tcaagcaaag tgacataatt gttgaattta tagtaactga aacgtatgat
     1741 tagggtgtat attaattatg agatggaaat caatgattgt tggctatgcg agagtatcat
     1801 cgaacgatca aaatttagaa agacaacttg aaaatttaaa gacgttttgt gctgaaaaaa
     1861 tatttacaga aaaacagtct gggaaatcta ttgaaaatag accagtactt caagaagcac
     1921 ttaacttttt gaggatggga gatcggtttg tagtagaatc cattgattgt ttaggtcgta
     1981 attatgatga agtgattcat accgttaatt atttaaaaga caaagaagta caattaatga
     2041 ttactagctt acctatgatg aatgaagtgg ttggcaatcc attactggat aagtttatga
     2101 aagatttaat tattcaaata ttagcaatgg tttcagaaca agagagaaat gaaagtaaac
     2161 gtcgacaagc gcaaggaatt caagttgcga aggaaaaagg cgtatataaa gggcgcccct
     2221 tactttattc accaaatgct aaagatccac agaaacgtat tatttatcat cgcgttgtag
     2281 aaatgttaga agaaggccaa tcaattagta aaattgcgaa agaagtaaat ataacaaggc
     2341 agacgattta cagaattaaa aacgataatg atttgattta ataaactttg tttcaatgca
     2401 actgagagct tctcacagcg tctcaaaaca gatgattata atctattatg agtttttaat
     2461 aataagacaa gaaaagagag cgtaaatatg ctctctcttt ttctcattaa cgaatgttta
     2521 taacgcaatc atgatgagag tattaataca gttaattatt tgaaatactt tagtttatat
     2581 aatgttgaat tataggtagt gaaattgtat ttattataca ttgtcatatc gttgtttaat
     2641 gatagttaat ttaaggataa tagaattaat attatatata gatgaacaaa acaagcaaca
     2701 gagcagaaaa aacactttta gattattttt actcattctc tattttattt atttttgcta
     2761 taataaataa agaatattaa atggaggtgt ttttatgaaa aagaaatttg tttctagttg
     2821 tattgctagt acaatcttat tcggcacttt attaggtgta acttataaag ctgaagctgc
     2881 aacagtacat gtagctggtg gtgtttggag ccatggtatt ggaaaacatt acgtatggtc
     2941 ttattatagt cacaataaaa gaaatcatgg ttcaacagca gtagggaaat attcatcttt
     3001 tagtggtgtt gctcgacctg gtgttcaatc taaggcatct gctccaaagg cttggggtgg
     3061 aaacaaaaca ttttatagtc ttcactaatt acatatatta attaaggaga atagacagaa
     3121 ttaagtaaac ttaattctgt ctattctctt gaaagaggca atatgaaaaa attcaaatta
     3181 tttttagatt ttagtacttt attattaatt tcagggctat tatttttatt tttcttcaaa
     3241 gaaaatgaag aaattatacc tgaaagttcc aatattttaa caatctctaa ttgggataaa
     3301 tctaacagta aatcaaaagt gttagatgtc attgaatcag gcgccaaaaa tcaaaatata
     3361 caaataatta aatccgttaa agattttgat aataaaaaag aattttttgt tttcaactca
     3421 aaacgaaata attctgactt tatacgtaat aaaacatcac ttttaactcc atcagattta
     3481 ttaaacagag agataaaagg aaagtattat attattggtg aacatttcaa tgtagaagag
     3541 cttacgagtg aattaaaaaa agctggttta acatctgaag ttgaatacat aaatagaaac
     3601 atattatttt ttgaattagt catagataat gatttattag tgcctttcat attcttaggc
     3661 gtattatatc ttttgtactt cttatatgac agaggatata atttgaaatt ttatgctatt
     3721 caaaagctac atggcttttc aactacacgc attatttttc ataatattca tataaaaatc
     3781 atttattggc tagtacttac cgctattttc tttattgcta atatattaat cattcatata
     3841 gccaatttag aattggattt cttaatgttt attcgtagat ttatcgtgtt gctttttgtt
     3901 tttgcttgta ttactacatt gctgtggctg atttcgtata gtcttctttt gaagttaagt
     3961 attccattaa tcttaaaagg gaaaaaagga tataagtttt caatatttat gggtaccttc
     4021 accaaatgta tcatggtgct aatactatct tttttactgg cacaaaattt aaatgcttat
     4081 acgaagttaa agaatatata tgactctgaa aaaatctggc aaaagttaga taattactat
     4141 acattagaga tttctcctaa tattagaaat tcagaagaaa aacaaatttt agaaactaat
     4201 atatacaatt taattaagaa aagcgaacaa cttggtggat tgcttttaaa gaataataat
     4261 atagccaatc ccgataaaaa caattacatt cctgaaaatg gcaatgtctt ttttatcaat
     4321 aacaattttc tgaatttcta taaaaatata aatcatgatt ttaaaatgat tgacaatcat
     4381 tcggataaaa taaacgtgtt tattcctcca aggttacaat atcaaaaatc tgaaattcaa
     4441 aaaaatcacc aaggatggat tgactttcaa aagcaacaaa acaaaaaagt agatgtgcaa
     4501 gtactgtcaa aaaacgtatc tgttttttct tttgatcgca caactaattt gaaatttggg
     4561 tatctagatt ctccaatagt aatgttgttg acacttgacg atttaacagg agatttttat
     4621 ttagctgctg tttctcaagg tggatatcta tttaaagatt tagatacact taaacagttg
     4681 cttaaagata atcacctaga agaagaaatt agcggtataa ctaattacaa ggattctgtg
     4741 ttaaatgaat taaatgaaac gaaaacaaaa atgataataa caatagttac tattattatt
     4801 aac
//
LOCUS       DAHHRR010000340         4542 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.guided.158, whole genome shotgun sequence.
ACCESSION   DAHHRR010000340 DAHHRR010000000
VERSION     DAHHRR010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.guided.158"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            <1..774
                     /locus_tag="NBI42_002829"
     CDS             <1..774
                     /locus_tag="NBI42_002829"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCD1779150.1"
                     /translation="TELLCFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGPDMT
                     DFGHRALSDPEFNEQYKEQMKQFKQRILEDNTFAKQFGDLGNVYGKQWRDWVDKDGNH
                     FDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQR
                     SADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARES
                     FNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            963..1442
                     /locus_tag="NBI42_002830"
     CDS             963..1442
                     /locus_tag="NBI42_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD1779151.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1457..2296
                     /gene="fakB2"
                     /locus_tag="NBI42_002831"
     CDS             1457..2296
                     /gene="fakB2"
                     /locus_tag="NBI42_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCD1779152.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGTSYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGAEIISIHLSSGLSGTFNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSVGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2382..2915
                     /gene="msrA"
                     /locus_tag="NBI42_002832"
     CDS             2382..2915
                     /gene="msrA"
                     /locus_tag="NBI42_002832"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD1779153.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            2908..3336
                     /gene="msrB"
                     /locus_tag="NBI42_002833"
     CDS             2908..3336
                     /gene="msrB"
                     /locus_tag="NBI42_002833"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCD1779154.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3348..3848
                     /locus_tag="NBI42_002834"
     CDS             3348..3848
                     /locus_tag="NBI42_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1779155.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            3848..4069
                     /locus_tag="NBI42_002835"
     CDS             3848..4069
                     /locus_tag="NBI42_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCD1779156.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4251..>4542
                     /locus_tag="NBI42_002836"
     CDS             4251..>4542
                     /locus_tag="NBI42_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCD1779157.1"
                     /translation="MDDKQHTSSSDDERAGIATSNQDQQTNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQSSD"
BASE COUNT         1674 a          661 c          813 g         1394 t
ORIGIN      
        1 accgaattat tatgcttcat taaaggagat acaaacatcc aatacttatt aaaatataat
       61 aataatattt ggaacgaatg ggcttttgaa aattatatca aatcagacga gtataaaggt
      121 ccagatatga ctgattttgg gcatcgtgca ttgagtgatc ctgaatttaa cgaacaatat
      181 aaagaacaaa tgaaacaatt taagcaacgt attcttgaag ataatacatt tgcgaagcaa
      241 ttcggtgatt taggaaatgt ttatggtaaa caatggcgag attgggttga taaagatggt
      301 aatcattttg atcaacttaa aacagtaatt gagcaaatta agcataatcc agattcaaga
      361 cgacacatcg tatctgcatg gaatccaaca gaaattgaca caatggcact tccgccttgt
      421 cataccatgt tccagtttta tgtccaagat ggtaagttaa gttgccagtt ataccaacgt
      481 agcgcagata tctttttagg tgtgccattt aatatcgcaa gctacgcttt attaacacac
      541 cttattgcca aagaatgtgg acttgaagta ggagaatttg tgcatacatt tggagatgca
      601 catatttatt caaatcatat tgatgcgatt caaacacaat tagcacgtga aagcttcaat
      661 cctccaacat taaaaattaa cagtgataag tctattttcg acataaatta tgaagatttg
      721 gaaattgttg actatgaatc acatccagca ataaaagctc caatagcagt gtagtcattg
      781 catagttagc taactatata gacatcaaaa tattttcaag tgcaaaaatg tacttttttg
      841 tgttaaacgt tttcataaat tatgcaaaat cattatttct atcacacttt atgataaaaa
      901 ttgtgttaaa ttaaagataa cttagtaata aaaaatgaaa tgatagaaga aggaggataa
      961 ttatgacttt atccattcta gttgcacatg acttgcaacg agtaattggt tttgaaaatc
     1021 aattaccttg gcacctacca aatgatttga agcatgttaa aaaattatca acaggtcata
     1081 ctttagtaat gggtcgtaag acatttgaat cgattggtaa accactaccg aatcgtcgaa
     1141 atgttgtact tacttcagat acaagtttca acgtagaggg cgttgatgta attcattcta
     1201 ttgaagatat ttaccaacta ccgggccatg ttttcatatt tggagggcaa acattatttg
     1261 aagaaatgat tgataaagtg gacgacatgt atattactgt tattgaaggt aaattccgtg
     1321 gtgatacgtt ctttccacct tatacatttg aagactggga agttgcctct tcagttgaag
     1381 gtaaactaga tgaaaaaaat acaattccac atacctttct acatttaatt cgtaaaaaat
     1441 aagggggaaa atgaccatga caaaacagat tatagtgaca gactcaacat ccgatttatc
     1501 taaagaatat ttagaagcaa acaacataca tgtaatccct ttaagtttaa ctattgaagg
     1561 aacttcatac gttgaccaag tagatattac atcagaagaa tttattaatc atattgaaaa
     1621 tgatgaagat gtaaagacaa gtcaaccagc cataggtgaa tttatatctg cttatgaaga
     1681 actaggaaaa gatggcgctg aaatcataag tattcatctt tcttcaggat taagtggtac
     1741 atttaacact gcttatcaag caagtcaaat ggtagatgct aatgtaactg ttattgattc
     1801 aaaatctatt tcttttggtt tagggtatca aatacaacac ctagtagagc ttgtaaaaga
     1861 aggtgtctca acttctgaaa tagttaaaaa gttaaatcat ttaagagaaa acattaaatt
     1921 atttgtagtt atagggcaat tgaatcaatt aattaaaggt ggcagaatta gtaaaacaaa
     1981 aggtttgatt ggtaatctta tgaaaatcaa accaattggt acactagatg atggtcgctt
     2041 agagcttgtg cacaatgcga gaactcaaaa ttctagtatc caatacttga aaaaggaaat
     2101 tgctgaattt ataggagatc atgaaatcaa atccgttggt gtcgcacatg ctaacgtcat
     2161 tgaatatgtt gataaattga agaaagtttt taatgaagct tttcatgtga ataattacga
     2221 tataaatgta actacaccag ttatttctgc acatactggt caaggtgcga ttggcctcgt
     2281 agtccttaag aagtaaattt aatcttttca gtgttaatta cttccatttc aatcctttat
     2341 agactaaatt tataattaga tagatagagg aggtaattca tatgacaaaa gaatatgcaa
     2401 cattagcagg aggatgtttc tggtgcatgg ttaaaccatt tacatcatat ccaggcatca
     2461 agtcagtcgt atctggttat agtggcggtc atgttgacaa cccaacttat gaacaggtat
     2521 gtacgaatca aaccggccat gtcgaagcag tacaaattac gtttgatcca gaggttactt
     2581 cctttgaaaa tatattagac atatatttca aaacatttga cccaactgat gatcaagggc
     2641 aatttttcga tagaggcgaa agctatcaac cagtcatttt ctatcatgat gagcatcaga
     2701 aaaaggctgc tgagtttaaa aagcaacaat taaatgaaca aggtattttc aagaaaccag
     2761 tgattacacc tattaaacca tataaaaatt tctatccagc tgaagactac catcaagatt
     2821 attataaaaa gaacccggta cattattacc aatatcaacg tggttcaggt agaaaagcgt
     2881 ttatagaatc acattggggg aatcaaaatg cttaaaaaag ataaaagtga actaacagac
     2941 atagaatata ttgttacaca agaaaatggc actgaaccac catttatgaa tgaatattgg
     3001 aatcattttg ctaaaggaat ttatgtagat aagatttctg gtaaaccttt atttacatct
     3061 gaagaaaagt ttcattctga atgtggatgg cctagctttt ccaaagcgct tgatgacgat
     3121 gaaattatag aattagtcga caaatcattt ggtatgttga gaactgaagt gcgttcagaa
     3181 gaatcaaata gtcatttagg acatgtcttt aatgatggac ctaaagaaag tggcggctta
     3241 agatactgta tcaattccgc tgcaattcaa tttattccat atgaaaaatt agaagaattg
     3301 ggttatggcg atttaatatc acattttgat aagtaggagt ggaaagtatg tttaaaaaat
     3361 tattcggaaa aggcaaagaa gttcagaaag atatcgcaat ttatgcacca ttaactggag
     3421 aatacgtgaa aattgaagat attccagatc ctgtattcgc acaaaaaatg atgggcgaag
     3481 ggtttggtat taatccaact gaaggagaag ttgtgtctcc aattgcagga cgtgttgata
     3541 atgtctttcc aactaagcat gcgattggtt taaaagcaga taatggatta gaattattag
     3601 ttcatatcgg tttagacaca gttcaattag acggtgaagg atttgaagtg ttagtgtcta
     3661 gtggtgacga agttaacgtc ggagatccat tagtaagatt caaccttgaa tatatcaata
     3721 ataacgctaa atctgttatt tcaccaatta ttattactaa cacagatcaa gcggcatcaa
     3781 ttaatattta tgatgaaaat gctgtgatta aaggtgaaac aaaagtgatt gatgtgacaa
     3841 tgaactaatg aaaaactatt cgttttatca atttgtcatg acagttcgtg ggcgacacga
     3901 tgataaaggt cgtctagcag aagagatatt tgacgatctt gctttcccaa aacacgatga
     3961 tgattttaac atactgtctg attatattga gacacatggt gatttcacat tgccaatgtc
     4021 tgtatttgat gatttatatg aagaatatac ggaatggcta aaattttaat ataataatag
     4081 ttagaaccag ggtgatgcaa gttgttatcc tggttttaat ttaaaataaa ctaagttgtg
     4141 actaaaaatt aatcaattat agtgaaatat ggtgcgctat cttgcataaa ttgatatgat
     4201 taactacaca gaatttaaaa gtacataata cataataagg aagtgataca atggatgata
     4261 agcaacacac atcttcatcc gatgatgaac gcgctggaat tgctacaagc aatcaagacc
     4321 aacaaactaa ttcgtcgaaa cgcgttcact taaaacgttg gcaattcata tcaattttaa
     4381 ttggtacaat cctaataaca gctgtcatca cagttgttgc atatattttt ataaatcaaa
     4441 aaataagtgg tttaaacaaa actgatcaag caaacttaaa taaaattgaa aatgtgtata
     4501 aaatcttaaa tagtgattat tataaaaaac agagctctga ca
//
LOCUS       DAHHRR010000341         4187 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.guided.163, whole genome shotgun sequence.
ACCESSION   DAHHRR010000341 DAHHRR010000000
VERSION     DAHHRR010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.guided.163"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            60..785
                     /gene="sasD"
                     /locus_tag="NBI42_002837"
     CDS             60..785
                     /gene="sasD"
                     /locus_tag="NBI42_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCD1779158.1"
                     /translation="MKKLATVGSLIVTSTFVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(976..1731)
                     /locus_tag="NBI42_002838"
     CDS             complement(976..1731)
                     /locus_tag="NBI42_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1779159.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            1967..2674
                     /gene="deoD"
                     /locus_tag="NBI42_002839"
     CDS             1967..2674
                     /gene="deoD"
                     /locus_tag="NBI42_002839"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD1779160.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            2681..4033
                     /gene="tet(38)"
                     /locus_tag="NBI42_002840"
     CDS             2681..4033
                     /gene="tet(38)"
                     /locus_tag="NBI42_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCD1779161.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDFNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
     gene            4114..>4187
                     /locus_tag="NBI42_002841"
     CDS             4114..>4187
                     /locus_tag="NBI42_002841"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HCD1779162.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQI"
BASE COUNT         1390 a          659 c          741 g         1397 t
ORIGIN      
        1 cttttgtaat catatgataa tgtaaatgta atcaaattgt aatataaagg gaaaagacaa
       61 tgaaaaaatt agcaacagta ggttctttaa ttgtaacaag cacttttgta ttctcaagta
      121 tgccttttca aaatgcgcat gctgacacaa cttcaatgaa tgtgtcgaat aaacaaagcc
      181 aaaatgtaca aaatcatcgc ccttatggcg gagtagtacc acaaggaatg acgcaagcac
      241 aatatactga attagagaaa gctttacccc aattaagcgc tggcagtaat atgcaagact
      301 ataatatgaa attgtatgat gcgacgcaaa atattgctga taaatacaat gtgataatta
      361 caactaatgt aggggtattt aaaccacatg ctgttagaga tatgaatgga catgcgttac
      421 ctttaacaaa agatggcaat ttttatcaaa cgaatgtaga tgcaaatggt gttaatcatg
      481 gtggtagtga aatggtgcaa aataaaacag gtcatatgag tcaacaaggt catatgaatc
      541 agaacacaca catgaaccaa cagccacaca tgcaacaagg tcatatgcaa tcatcaaacc
      601 atcaaatgat gagtccaaaa gcaaatatgc attcatcaaa tcatcaaatg aaccaaagta
      661 acaaaaaagt tttaccagct gctggtgaaa gtatgacatc aagtattctt actgcaagta
      721 ttgccgcact actattagta tctgggttat tcttagcatt tagacgacgt tcaacaaata
      781 aataaacata atacgattaa taatagaaaa atcgtgtgat tatctgagcg agcctaggac
      841 ataaatcaat gtcctaggct cgctaatgtt atattggcag tagttgactg aatgaaattg
      901 cgcttgtaac aagcttttcc atttcttgcc cacttcctaa ataatcatca caaaatttct
      961 tatatttcaa taattttaat aatccgattg tcttatacgt gtcagtgtta attcagagat
     1021 ttcctgtgga atataccact tattaatcat aattggataa ggtgtttgtg cgtacaatgt
     1081 ttcaataatc agccaacaat gtgtatcacc atcaaacacg tgactatgat ttttgaagtg
     1141 gggcgctttg gtaatagaca tttttaaatc tgattgatat gcattgctat aaatcgtttg
     1201 ctcaacgaac gtcttcatgt cgtcttcgtt ttgtgtattc actttaaatg tgtcaatgac
     1261 atttaacggt ataaaggtaa agcaaaatgc atcagcttgc ttagaatgat tgtccttttt
     1321 ttgataatag cgttccattg caatgacggc agaaggatgg tttgcaaaca aatgatttgt
     1381 atattcactt tctaaatcaa cacgataatt aattgatgac atagatacgc gagctagcaa
     1441 tatttgatca agtggatgct taaattgatc catacttgaa gcgtgttggg catttgtttg
     1501 tggaataaca aagtgtccct tccctcttgt actctctacg atgccatctt cggctaacaa
     1561 ttttatagct tggcgcaaag tcatacgact gacatcaaag cgcgcacaaa gttccttttc
     1621 agtaggtaat gcatggccac tgggatattt tcctatttga atttctttat ataacgtatt
     1681 ataaatcgtt aaaaattttg gttgtgtttg cgtcacgtag acaacctcca taaagttact
     1741 taatcactct catcatacaa taatttttac tcgaattgga aaaattataa aaattaaaca
     1801 tagatagact ttgaaaatta gttttataca aggttagtag ctataactgt aaaatgttct
     1861 taatattgtc aaaatgtaat gcttgaaagc gcttttaaaa aatattatta tatacatggt
     1921 tagacaaata gacaaatcac tatacaaata ttgggaggaa tattttatga aatcaacacc
     1981 acacattaaa ccaatgaatg acgtcgaaat tgcagaaacg gttctattgc caggagatcc
     2041 gttaagagct aagttcattg cagaaacata tttggacgat gtggaacagt tcaatacagt
     2101 gcgaaacatg tttggtttta ccggaacata taaaggtaaa aaagtttctg tcatgggttc
     2161 aggaatgggt atgccatcaa ttggcattta ctcttatgaa ttaattcata catttggttg
     2221 taaaaaatta attcgcgttg gttcttgtgg cgcgatgcaa gaaaacattg atttatatga
     2281 tgtgattatt gcacaaggtg cctctactga ttcaaattac gttcaacaat atcaattacc
     2341 aggtcatttt gcgccaattg cttcttatca gttattagaa aaagcagttg aaacagcacg
     2401 tgacaaaggt gtacgtcatc atgtaggtaa tgtgttatca agtgatattt tctataacgc
     2461 ggatacaaca gcgagtgaac gttggatgcg tatgggtatt ttaggtgtag aaatggaatc
     2521 agccgcatta tacatgaatg caatttacgc tggtgtcgaa gcattaggcg tgttcacagt
     2581 gagcgatcat ttaattcatg aaacgtcaac aacacctgag gaaagggaac gtgcatttac
     2641 agatatgatt gaaattgcac tgtcattggt gtagatgatt atgaatgttg aatattctaa
     2701 aataaagaaa gcagtaccta ttttattatt cttatttgta ttcagtttgg ttatagacaa
     2761 ctcatttaaa ttgatttctg tagccattgc tgatgactta aacatttctg taacgacagt
     2821 aagttggcaa gcgacattag ccggtttagt aattggtatt ggcgctgtag tatacgcttc
     2881 tttatctgat gccattagta tacgcacact atttatttat ggcgtgatat taatcattat
     2941 tggatcaatt attggttaca ttttccaaca tcaattcgca ttacttttag ttggacgtat
     3001 tattcaaact gccggtttag ctgctgcaga gacattatat gtgatatatg ttgcaaagta
     3061 tctttctaaa gaggaccaga agacttacct tggcttaagt acgagcagtt attccttgtc
     3121 attagttatc ggtacattat caggtggatt tatttctacg tatttacact ggacaaatat
     3181 gtttttaatt gcattaatcg tagtatttac gttgccattc ctatttaaat tattaccaaa
     3241 agaaaataat acaaataaag ctcatttaga ttttgttggc ttaattctag tggcaactat
     3301 cgctacaaca gtcatgctgt ttattacgaa ctttaattgg ttatatatga ttggtgcctt
     3361 aattgcgatt atcgtttttg cgctatatat taaaaatgct caacgtccat tagtaaacaa
     3421 atcatttttc caaaataaac gttatgcttc atttttattt atagtattcg taatgtatgc
     3481 tatccaattg ggttatattt ttacgttccc attcataatg gaacaaattt atcatcttca
     3541 gctagacaca acatcactgt tattagtacc gggttatatt gtagcagtca tcgttggtgc
     3601 actaagtggt aaaatcggcg aatatcttaa ttcaaaacaa gcgattatca cagcaattat
     3661 tttaatcgca ctgagcttga ttttacctgc atttgcagta ggtaatcaca tttcaatctt
     3721 cgtcatttct atgatattct ttgcaggtag ctttgcttta atgtatgcac ctttacttaa
     3781 cgaagccatt aaaacaatag attttaatat gacaggtgtg gctattggct tttataattt
     3841 aattattaat gtggcggtat ctgtaggtat tgcgattgct gcggctctaa tcgattttaa
     3901 agcattaaat ttcccaggca atgatgcatt aagttcacat ttcggtatta ttttaattat
     3961 tttaggttta atgagtattg tcggattagt tttattcgtc atcttaaatc gttggacaca
     4021 atctgaaaaa taaatagata taaattcgcg agatatattg gtatttatag taaaattaaa
     4081 taaagagatt atataacacg aggagtagta agtatgaaat ttgagaaata tatagatcac
     4141 actttattga agcctgagtc aacacgtacg caaatcgatc aaatcat
//
LOCUS       DAHHRR010000342         2104 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.guided.206, whole genome shotgun sequence.
ACCESSION   DAHHRR010000342 DAHHRR010000000
VERSION     DAHHRR010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.guided.206"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            20..2029
                     /gene="mecA"
                     /locus_tag="NBI42_002842"
     CDS             20..2029
                     /gene="mecA"
                     /locus_tag="NBI42_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCD1779163.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
BASE COUNT          884 a          285 c          360 g          575 t
ORIGIN      
        1 gtcttatata aggagtatat tgatgaaaaa gataaaaatt gttccactta ttttaatagt
       61 tgtagttgtc gggtttggta tatattttta tgcttcaaaa gataaagaaa ttaataatac
      121 tattgatgca attgaagata aaaatttcaa acaagtttat aaagatagca gttatatttc
      181 taaaagcgat aatggtgaag tagaaatgac tgaacgtccg ataaaaatat ataatagttt
      241 aggcgttaaa gatataaaca ttcaggatcg taaaataaaa aaagtatcta aaaataaaaa
      301 acgagtagat gctcaatata aaattaaaac aaactacggt aacattgatc gcaacgttca
      361 atttaatttt gttaaagaag atggtatgtg gaagttagat tgggatcata gcgtcattat
      421 tccaggaatg cagaaagacc aaagcataca tattgaaaat ttaaaatcag aacgtggtaa
      481 aattttagac cgaaacaatg tggaattggc caatacagga acagcatatg agataggcat
      541 cgttccaaag aatgtatcta aaaaagatta taaagcaatc gctaaagaac taagtatttc
      601 tgaagactat atcaaacaac aaatggatca aaattgggta caagatgata ccttcgttcc
      661 acttaaaacc gttaaaaaaa tggatgaata tttaagtgat ttcgcaaaaa aatttcatct
      721 tacaactaat gaaacagaaa gtcgtaacta tcctctagga aaagcgactt cacatctatt
      781 aggttatgtt ggtcccatta actctgaaga attaaaacaa aaagaatata aaggctataa
      841 agatgatgca gttattggta aaaagggact cgaaaaactt tacgataaaa agctccaaca
      901 tgaagatggc tatcgtgtca caatcgttga cgataatagc aatacaatcg cacatacatt
      961 aatagagaaa aagaaaaaag atggcaaaga tattcaacta actattgatg ctaaagttca
     1021 aaagagtatt tataacaaca tgaaaaatga ttatggctca ggtactgcta tccaccctca
     1081 aacaggtgaa ttattagcac ttgtaagcac accttcatat gacgtctatc catttatgta
     1141 tggcatgagt aacgaagaat ataataaatt aaccgaagat aaaaaagaac ctctgctcaa
     1201 caagttccag attacaactt caccaggttc aactcaaaaa atattaacag caatgattgg
     1261 gttaaataac aaaacattag acgataaaac aagttataaa atcgatggta aaggttggca
     1321 aaaagataaa tcttggggtg gttacaacgt tacaagatat gaagtggtaa atggtaatat
     1381 cgacttaaaa caagcaatag aatcatcaga taacattttc tttgctagag tagcactcga
     1441 attaggcagt aagaaatttg aaaaaggcat gaaaaaacta ggtgttggtg aagatatacc
     1501 aagtgattat ccattttata atgctcaaat ttcaaacaaa aatttagata atgaaatatt
     1561 attagctgat tcaggttacg gacaaggtga aatactgatt aacccagtac agatcctttc
     1621 aatctatagc gcattagaaa ataatggcaa tattaacgca cctcacttat taaaagacac
     1681 gaaaaacaaa gtttggaaga aaaatattat ttccaaagaa aatatcaatc tattaactga
     1741 tggtatgcaa caagtcgtaa ataaaacaca taaagaagat atttatagat cttatgcaaa
     1801 cttaattggc aaatccggta ctgcagaact caaaatgaaa caaggagaaa ctggcagaca
     1861 aattgggtgg tttatatcat atgataaaga taatccaaac atgatgatgg ctattaatgt
     1921 taaagatgta caagataaag gaatggctag ctacaatgcc aaaatctcag gtaaagtgta
     1981 tgatgagcta tatgagaacg gtaataaaaa atacgatata gatgaataac aaaacagtga
     2041 agcaatccgt aacgatggtt gcttcactgt tttattatga attattaata agtgctgtta
     2101 cttc
//
LOCUS       DAHHRR010000343         1553 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M97081
            SAMN28743350-rid14506313.guided.237, whole genome shotgun sequence.
ACCESSION   DAHHRR010000343 DAHHRR010000000
VERSION     DAHHRR010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28743350
            Sequence Read Archive: SRR19441898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001316885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001316885.1
            Genome Coverage       :: 94x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:50:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 51 of 78
            Pseudo Genes (incomplete)         :: 24 of 78
            Pseudo Genes (internal stop)      :: 25 of 78
            Pseudo Genes (multiple problems)  :: 19 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28743350-rid14506313.guided.237"
                     /isolate="M97081"
                     /isolation_source="Abscess Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-07-02"
     gene            91..1077
                     /locus_tag="NBI42_002843"
     CDS             91..1077
                     /locus_tag="NBI42_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCD1779164.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1049..1309
                     /locus_tag="NBI42_002844"
     CDS             1049..1309
                     /locus_tag="NBI42_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCD1779165.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(1300..>1553)
                     /locus_tag="NBI42_002845"
     CDS             complement(1300..>1553)
                     /locus_tag="NBI42_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCD1779166.1"
                     /translation="VFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAE
                     NNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC"
BASE COUNT          558 a          214 c          256 g          525 t
ORIGIN      
        1 ccttatataa gactacattt gtagtatatt acaaatgtag tatttatgtc aaaataatgt
       61 tataattttt gtgatatgga ggtgtagaag gtgttatcat cttttttaat gttaagtata
      121 atcagttcat tgctcacgat atgtgtaatt tttttagtga gaatgctcta tataaaatat
      181 actcaaaata ttatgtcaca taagatttgg ttattagtgc tcgtctccac gttaattcca
      241 ttaataccat tttacaaaat atcgaatttt acattttcaa aagatatgat gaatcgaaat
      301 gtatctgaca cgacttcttc ggttagtcat atgttagatg gtcaacaatc atctgttacg
      361 aaagacttag caattaatgt taatcagttt gagacctcaa atataacgta tatgattctt
      421 ttgatatggg tatttggtag tttgttgtgc ttattttata tgattaaggc attccgacaa
      481 attgatgtta ttaaaagttc gtcattggaa tcgtcatatc ttaatgaacg acttaaagta
      541 tgtcaaagta agatgcagtt ctacaaaaag catataacaa ttagttatag ttcaaacatt
      601 gataatccga tggtatttgg tttagtgaaa tcccaaattg tactaccaac tgtcgtagtc
      661 gaaaccatga atgacaaaga aattgaatat attattctac atgaactatc acatgtgaaa
      721 agtcatgact taatattcaa ccagctttat gttgttttta aaatgatatt ctggtttaat
      781 cctgcactat atataagtaa aacaatgatg gacaatgact gtgaaaaagt atgtgataga
      841 aacgttttaa aaattttgaa tcgccatgaa catatacgtt atggtgaatc gatattaaaa
      901 tgctctattt taaaatctca gcacataaat aatgtggcag cacaatattt actaggtttt
      961 aattcaaata ttaaagaacg tgttaagtat attgcacttt atgattcaat gcctaaacct
     1021 aatcgaaaca agcgtattgt tgcgtatatt gtatgtagta tatcgagctt cacatgaaac
     1081 agctaaagaa gctttgggcg ataaagagtt aagagccatt gcacatgagt taactaaaac
     1141 agttaaggat aacatgagtg ttgattggtc taaacgagac agtgctaaag ctaaaatgag
     1201 agttcaagtt agacgcctat taaagaaata tggctatcca ccagatcttc aaaaaatggc
     1261 tgtggaacaa gttgtagagc aagcagaatt aatggcaagt cagcaataaa aaaataaatc
     1321 ataatgagtc cgggacataa agttcttgga taagtgaaaa aagacaattt ctattgaaat
     1381 aatatagaaa ttgtcttttt tataaatttt ttgattattt tcagctcgtt gagctactac
     1441 ttttcttata ttaagtgcca ttaatacaaa accaagttct cttttgactt tattgagtcc
     1501 tcggacagac atccgagtga aacccaaaat agccttcata aatccaaaaa cag
//
